LOCUS       DAZOYL010000001        58899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZOYL010000001 DAZOYL010000000
VERSION     DAZOYL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.001"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(153..1100)
                     /gene="dusC"
                     /locus_tag="ACHUOY_000001"
     CDS             complement(153..1100)
                     /gene="dusC"
                     /locus_tag="ACHUOY_000001"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR0680908.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1339..1737
                     /locus_tag="ACHUOY_000002"
     CDS             1339..1737
                     /locus_tag="ACHUOY_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR0680909.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1734..2429
                     /locus_tag="ACHUOY_000003"
     CDS             1734..2429
                     /locus_tag="ACHUOY_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR0680910.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2559..3443
                     /gene="cdd"
                     /locus_tag="ACHUOY_000004"
     CDS             2559..3443
                     /gene="cdd"
                     /locus_tag="ACHUOY_000004"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR0680911.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3593..4312
                     /gene="sanA"
                     /locus_tag="ACHUOY_000005"
     CDS             3593..4312
                     /gene="sanA"
                     /locus_tag="ACHUOY_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR0680912.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4315..4554
                     /locus_tag="ACHUOY_000006"
     CDS             4315..4554
                     /locus_tag="ACHUOY_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR0680913.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4748..5986
                     /gene="preT"
                     /locus_tag="ACHUOY_000007"
                     /pseudo
     CDS             4748..5986
                     /gene="preT"
                     /locus_tag="ACHUOY_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            5980..7215
                     /gene="preA"
                     /locus_tag="ACHUOY_000008"
     CDS             5980..7215
                     /gene="preA"
                     /locus_tag="ACHUOY_000008"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR0680914.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7342..8352)
                     /gene="mglC"
                     /locus_tag="ACHUOY_000009"
     CDS             complement(7342..8352)
                     /gene="mglC"
                     /locus_tag="ACHUOY_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR0680915.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8368..9888)
                     /gene="mglA"
                     /locus_tag="ACHUOY_000010"
     CDS             complement(8368..9888)
                     /gene="mglA"
                     /locus_tag="ACHUOY_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR0680916.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9949..10947)
                     /gene="mglB"
                     /locus_tag="ACHUOY_000011"
     CDS             complement(9949..10947)
                     /gene="mglB"
                     /locus_tag="ACHUOY_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR0680917.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11227..12225)
                     /gene="galS"
                     /locus_tag="ACHUOY_000012"
     CDS             complement(11227..12225)
                     /gene="galS"
                     /locus_tag="ACHUOY_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR0680918.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            complement(12409..13566)
                     /gene="yeiB"
                     /locus_tag="ACHUOY_000013"
     CDS             complement(12409..13566)
                     /gene="yeiB"
                     /locus_tag="ACHUOY_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR0680919.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13583..14251)
                     /gene="folE"
                     /locus_tag="ACHUOY_000014"
     CDS             complement(13583..14251)
                     /gene="folE"
                     /locus_tag="ACHUOY_000014"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR0680920.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14509..15345
                     /gene="yeiG"
                     /locus_tag="ACHUOY_000015"
     CDS             14509..15345
                     /gene="yeiG"
                     /locus_tag="ACHUOY_000015"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0680921.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15377..17368)
                     /gene="cirA"
                     /locus_tag="ACHUOY_000016"
     CDS             complement(15377..17368)
                     /gene="cirA"
                     /locus_tag="ACHUOY_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR0680922.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17660..19129)
                     /gene="lysP"
                     /locus_tag="ACHUOY_000017"
     CDS             complement(17660..19129)
                     /gene="lysP"
                     /locus_tag="ACHUOY_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR0680923.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19334..20215)
                     /gene="yieE"
                     /locus_tag="ACHUOY_000018"
     CDS             complement(19334..20215)
                     /gene="yieE"
                     /locus_tag="ACHUOY_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR0680924.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20314..21363
                     /locus_tag="ACHUOY_000019"
     CDS             20314..21363
                     /locus_tag="ACHUOY_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR0680925.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21437..22294
                     /gene="nfo"
                     /locus_tag="ACHUOY_000020"
     CDS             21437..22294
                     /gene="nfo"
                     /locus_tag="ACHUOY_000020"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR0680926.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22298..23386
                     /locus_tag="ACHUOY_000021"
     CDS             22298..23386
                     /locus_tag="ACHUOY_000021"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0680927.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23442..24692)
                     /locus_tag="ACHUOY_000022"
     CDS             complement(23442..24692)
                     /locus_tag="ACHUOY_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0680928.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24792..25733)
                     /gene="rihB"
                     /locus_tag="ACHUOY_000023"
     CDS             complement(24792..25733)
                     /gene="rihB"
                     /locus_tag="ACHUOY_000023"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR0680929.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25863..26561
                     /locus_tag="ACHUOY_000024"
     CDS             25863..26561
                     /locus_tag="ACHUOY_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR0680930.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26628..27878)
                     /locus_tag="ACHUOY_000025"
     CDS             complement(26628..27878)
                     /locus_tag="ACHUOY_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0680931.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27972..28910)
                     /gene="psuG"
                     /locus_tag="ACHUOY_000026"
     CDS             complement(27972..28910)
                     /gene="psuG"
                     /locus_tag="ACHUOY_000026"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR0680932.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28898..29839)
                     /locus_tag="ACHUOY_000027"
     CDS             complement(28898..29839)
                     /locus_tag="ACHUOY_000027"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR0680933.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30297..31988)
                     /gene="fruA"
                     /locus_tag="ACHUOY_000028"
     CDS             complement(30297..31988)
                     /gene="fruA"
                     /locus_tag="ACHUOY_000028"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR0680934.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(32005..32943)
                     /gene="fruK"
                     /locus_tag="ACHUOY_000029"
     CDS             complement(32005..32943)
                     /gene="fruK"
                     /locus_tag="ACHUOY_000029"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR0680935.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32943..34073)
                     /gene="fruB"
                     /locus_tag="ACHUOY_000030"
     CDS             complement(32943..34073)
                     /gene="fruB"
                     /locus_tag="ACHUOY_000030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR0680936.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34441..35622
                     /gene="setB"
                     /locus_tag="ACHUOY_000031"
     CDS             34441..35622
                     /gene="setB"
                     /locus_tag="ACHUOY_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR0680937.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35619..35873)
                     /locus_tag="ACHUOY_000032"
     CDS             complement(35619..35873)
                     /locus_tag="ACHUOY_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR0680938.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36028..36600
                     /gene="yeiP"
                     /locus_tag="ACHUOY_000033"
     CDS             36028..36600
                     /gene="yeiP"
                     /locus_tag="ACHUOY_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR0680939.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36823..38289
                     /locus_tag="ACHUOY_000034"
     CDS             36823..38289
                     /locus_tag="ACHUOY_000034"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0680940.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38407..39393
                     /gene="yeiR"
                     /locus_tag="ACHUOY_000035"
     CDS             38407..39393
                     /gene="yeiR"
                     /locus_tag="ACHUOY_000035"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR0680941.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39432..40145
                     /gene="lpxT"
                     /locus_tag="ACHUOY_000036"
     CDS             39432..40145
                     /gene="lpxT"
                     /locus_tag="ACHUOY_000036"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR0680942.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40557..41123
                     /gene="mepS"
                     /locus_tag="ACHUOY_000037"
     CDS             40557..41123
                     /gene="mepS"
                     /locus_tag="ACHUOY_000037"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR0680943.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41304..42860
                     /locus_tag="ACHUOY_000038"
     CDS             41304..42860
                     /locus_tag="ACHUOY_000038"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR0680944.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42942..44756
                     /locus_tag="ACHUOY_000039"
     CDS             42942..44756
                     /locus_tag="ACHUOY_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR0680945.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44757..45851
                     /locus_tag="ACHUOY_000040"
     CDS             44757..45851
                     /locus_tag="ACHUOY_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR0680946.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45851..46876
                     /locus_tag="ACHUOY_000041"
     CDS             45851..46876
                     /locus_tag="ACHUOY_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0680947.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46878..48467
                     /gene="yejF"
                     /locus_tag="ACHUOY_000042"
     CDS             46878..48467
                     /gene="yejF"
                     /locus_tag="ACHUOY_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR0680948.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48471..48815)
                     /locus_tag="ACHUOY_000043"
     CDS             complement(48471..48815)
                     /locus_tag="ACHUOY_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR0680949.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49148..50205)
                     /locus_tag="ACHUOY_000044"
                     /pseudo
     CDS             complement(49148..50205)
                     /locus_tag="ACHUOY_000044"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(50296..50991)
                     /gene="rsuA"
                     /locus_tag="ACHUOY_000045"
     CDS             complement(50296..50991)
                     /gene="rsuA"
                     /locus_tag="ACHUOY_000045"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR0680950.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51141..52901
                     /locus_tag="ACHUOY_000046"
     CDS             51141..52901
                     /locus_tag="ACHUOY_000046"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0680951.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            53026..53310
                     /gene="rplY"
                     /locus_tag="ACHUOY_000047"
     CDS             53026..53310
                     /gene="rplY"
                     /locus_tag="ACHUOY_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR0680952.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53449..54456)
                     /gene="yejK"
                     /locus_tag="ACHUOY_000048"
     CDS             complement(53449..54456)
                     /gene="yejK"
                     /locus_tag="ACHUOY_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR0680953.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54638..54865
                     /locus_tag="ACHUOY_000049"
     CDS             54638..54865
                     /locus_tag="ACHUOY_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR0680954.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54885..56645
                     /gene="yejM"
                     /locus_tag="ACHUOY_000050"
     CDS             54885..56645
                     /gene="yejM"
                     /locus_tag="ACHUOY_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR0680955.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56720..56796
                     /locus_tag="ACHUOY_000051"
     tRNA            56720..56796
                     /locus_tag="ACHUOY_000051"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56754..56756,aa:Pro,seq:ggg)
     gene            complement(56899..58899)
                     /gene="yejO"
                     /locus_tag="ACHUOY_000052"
                     /pseudo
     CDS             complement(56899..58899)
                     /gene="yejO"
                     /locus_tag="ACHUOY_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        14504 a        15365 c        14377 g        14653 t
ORIGIN      
        1 ggtaacatcc ctgtaaaacg cgccctcagc ccacctccat gtgggctgcc ccggccttca
       61 gggaacgcct cggcaatttt gacgccacca aacaaccgtg cggcctattg ataaagagct
      121 aacacattgt caaaaaacat cactatggtt ttttagagtt tctcgatatc aattgcctga
      181 atagcccttg caatatcagg ggaattattc aacacccgaa catgctgaaa taattccgtt
      241 gcttcatcgt attctttacg caaataactc aaccactgtt taatccgcgc aacgtgatat
      301 aacccggtat cgccctgctt ttccagacgg gtatattttt gcagcaaagc aaccacctcc
      361 ggccacggca ttcgcggttc gttatatttt actacccggc tcaggttggg aatattgagc
      421 gccccgcgac caatcatcac tgcgtcgcag ccgctgatcg caatgcattg ttgcgcgctc
      481 tgccagtccc agatttcgcc gttggcaatc accggaatat tcagccgctg gcgaatctcg
      541 ccaatcgcct gccagtcaat atgctccgcg cggtaaccct gctcttttgt ccgtccatgc
      601 accaccagct ccgtagcacc agcctgttga accgcatcgg cgatttcaaa tttcttctca
      661 ccgctgtccc agcccagacg cactttcacg ctgacgggca aatgcgccgg tacagcttca
      721 cgcatcgctt ttgcaccctg gtagatgagt tcaggatctt tgagtaacgt cgccccgccg
      781 ccgctgccgt taaccgtttt cgacgggcag ccgcaattga gatccacgcc ccaggaacct
      841 aattccaccg cacgggcggc gttctctgcc agccattgtg ggaactgacc taacaactga
      901 acgcgcacca gcgtaccgga tggtgtccgg ctggcgtttt gtagctcagg gcaaatgcga
      961 tgaaagactt ttaccggcag cagttgatcc accacgcgga caaactcggt gatgcacaga
     1021 tcgtagtcgt taacttcggt cagcaattct cgcaccagag agtcaagcac accctccatc
     1081 ggagccagta acacacgcat atcttcaccc ggaaaaaaat gaggcgctat gttagcgcct
     1141 atggtcaacg gattaaaggt ctgcaaagag ggattgctgc aaactgtaat ccctctctat
     1201 gcttgatgta tgcctgagtt cgttgcgcat tgcgtaatgc cggggagcga aaaaataagc
     1261 gctgatttct taatgtgatc ggtagcacgt tttacgaatt aattgtatga tgaatccatc
     1321 tcatctgggg tgttgattat gagtaagaca ctgaacatta tctggcaata tttacgcgct
     1381 ttcgtcctga tttatgcctg cctgtatgca ggcattttca ttgcttccct gctaccggta
     1441 accattccgg gcagcatcat cgggatgctg atcctgtttg tcctgctggc cttgcaaatt
     1501 cttccggcaa aatgggtcaa tccggggtgc tacgtactga ttcgctatat ggcgctattg
     1561 tttgtgccga ttggcgtagg cgtcatgcaa tattttgatt tgctccgcgc acagtttggc
     1621 ccggtagtgg tttcctgtgc agtcagtacg ctggtggttt ttctggtggt gagctggagt
     1681 tcacaactgg tacacggtga acgtaaagtc gtaggtcaga aaggatcaga agaatgatgg
     1741 cgaatatctg gtggtcatta ccgctgacgt tgattgtctt ttttgccgcc cgcaaactgg
     1801 cggcacggta taagtttccc ctgcttaacc cgttgctggt agcaatggtg gtgatcattc
     1861 cttttttaat gctgaccggc atctcttacg acagctactt taaaggtagc gaagtgctaa
     1921 acgatctgct gcaaccggcg gtcgtcgcgc tggcctatcc tttatatgag cagctacacc
     1981 agatccgcgc gcgctggaaa tcgatcatca ccatctgttt tatcggcagc gtggttgcga
     2041 tggtgacggg gacttccgtg gcattgttga tgggcgcttc accggaaatc gccgcgtcaa
     2101 tcctgccgaa atcagtcacc acgcctattg caatggcggt tggcggcagt attggcggta
     2161 ttccggcaat cagcgcagtt tgcgtgattt tcgtcggcat cctcggcgcg gtatttggcc
     2221 atacattgct taatgcgatg cgtattcgta ccaaagctgc gcgcggtctg gcaatgggga
     2281 cagcctcgca cgccctcggt acggcgcgct gcgccgagct ggattatcag gaaggtgcat
     2341 ttagttcgct ggcgctggtg ttatgcggga taattacttc gctgatcgca ccgttccttt
     2401 tcccgattat tctggcagta atgggctaaa atttgcgatg cgtcgcgcat ttttgatgta
     2461 agtttcacgc gttgcataat taatgagatt cagatcacat ataaagccat aacgggttcg
     2521 taaactgtta tcccattaca tgattatgag gcaacgccat gcatccacgt tttcaaaccg
     2581 cttttgccca acttgcggat aacttgcaat ctgcactgga acctattctg gcagacaagt
     2641 acttccccgc tttgttgacc ggggagcaag tctcatcgct gaagagcgca acggggctgg
     2701 acgaagacgc gctggcattc gcactacttc cactggcggc ggcctgtgcg cgtacgccat
     2761 tgtcgaattt taatgttggc gcaattgcgc gcggtgtgag cggaacctgg tatttcggtg
     2821 ccaatatgga atttattggt gcaacaatgc agcaaaccgt tcatgccgaa caaagcgcga
     2881 tcagccacgc ctggttgagt ggtgaaaaag cgcttgcagc catcaccgtt aactatacgc
     2941 cttgtggtca ctgccgtcag tttatgaatg aactgaacag cggtctggat ctgcgtattc
     3001 atctgccggg ccgcgaggca cacgcgctgc gtgactatct gccggatgcc tttgggccga
     3061 aagatctgga gattaaaacg ctgctgatgg acgaacagga tcacggctat gcgctgacgg
     3121 gtgatgcgct ttctcaggca gcgattgcgg cggcaaaccg ttcgcacatg ccttacagta
     3181 agtcgccaag cggtgtcgcg ctggaatgta aagacggtcg tattttcagc ggcagctacg
     3241 ctgaaaacgc cgcattcaac ccgactctgc caccgttgca gggggcgtta attctgttga
     3301 atctcaaggg ttatgattac ccggatatcc agcgcgcggt tctggcagaa aaagccgatg
     3361 cgccgttgat tcagtgggat gccacctccg caacgctgaa agctctcggc tgtcacagta
     3421 tcgaccgcgt gcttctcgct taagcctggt gccggatgcg gcgtgaacgc cttgtccggc
     3481 ttgccagccc tctcttggtg tcgaaattcc cggcaaacag tttgccgttt cttgcgcaaa
     3541 accagcgggt aaagtagcct gatggaaatt ttccttagat cgagtctcct gcatgttaaa
     3601 gcgcgtgttc ctcagcctgt tagtcctgat cggcttgctg ctgttgactg tgctcggcct
     3661 cgatcgctgg atgagctgga aaaccgcgcc ttatatctac gacgaattgc aggatctccc
     3721 ctaccgccag gtcggcgtgg tgctcggcac agcaaaatat tatcgtactg gcgtaattaa
     3781 tcagtattat cgctaccgca ttcaaggagc gattaatgcc tataacagcg gtaaggtaaa
     3841 ttatctatta ctgagcggcg ataacgcatt gcaaagttat aatgagccga tgaccatgcg
     3901 caaagattta atcgctgctg gtgtcgaccc atcagatatt gttctcgatt acgcaggctt
     3961 tcgtacgctg gattccatcg tgcgtacacg caaagtgttc gatactaatg atttcattat
     4021 tatcacccaa cgtttccact gtgagcgggc attatttatt gcgctgcata tggggattca
     4081 ggctcagtgt tatgccgtac cgtcaccgaa agatatgctg tcagtacgta ttcgcgaatt
     4141 tgccgcccgt ttcggtgcgc tggctgacct ttatattttt agacgtgaac cgcgtttttt
     4201 agggccgctg gtccctattc cggctatgca ccaggtgccg gaagatgcgc aggggtatcc
     4261 cgccgtcaca cccgaacagt tacttgaatt acaaaagaaa caaggaaagt agttatggat
     4321 gtacagcagt tttttgtcgt tgccgttttt ttccttatcc cgatattttg tttccgcgaa
     4381 gcatggaaag gctggcgcgc aggcgcgatt gataaacggg ttaaaaatgc accggaaccg
     4441 gtgtatgtct ggcgagcaaa aaatcccgga ctctttttcg cttatatggt ggcatatatc
     4501 ggcttcggaa ttttatctat cggcatgatt gtttatctta ttttctatcg ttaatatttt
     4561 ctcttttctg ttaattattg agaataatta ttacttcact caataagctg cggatataac
     4621 attctgaacc gcagctattt gtgaatcctt tcacagttta aatttcccca cacgcttagc
     4681 cttaatatca gcacgcaata tttatttagc gttcgcctta attactcata gcattaagga
     4741 agatcacatg ccgcaacaaa attatctgga tgaactcact ccggctttta cgcctttact
     4801 ggcgattaaa gaagcctctc gctgtttatt atgtcatgac gctccctgta gtcaggcttg
     4861 cccggcacag accgatccgg ggaaatttat tcgctcaatc tactttcgta attttaaagg
     4921 cgctgccgag acaattcgcg aaaataatgc cctcggtgcc gtttgtgcca gagtgtgccc
     4981 gacggagaaa ttatgccaaa gcggttgtac ccgtgccggt gttgatgctc ccattgatat
     5041 cggccgctta cagcgttttg ttactgattt tgaacaacaa accgggatgg aaatttatca
     5101 gcccggtact aaaacgctcg gcaaagtcgc gattattggc gcaggtcctg ccggattgca
     5161 ggccagtgtg acactgacaa accagggtta tgacgtcacg atctatgaga aagaagcaca
     5221 acccggtggc tgattgcgta acggcattcc gcaattccgg ttaccgcaat cagtactgga
     5281 tgcagagatc gcccgtattg aaaaaatggg cgtgaccatt aagtgcaaca acgaagtcgg
     5341 taacacactc acccttgagc agctgaaagc agaaaaccgc gcggtactgg tcaccgtggg
     5401 gttatcaagc ggttccgggc taccgctgtt tgagcatagt gacgttgaga ttgccgtcga
     5461 cttcttgcaa cgtgcacgac aggcgcaagg cgatatcagc attccacaaa gcgcattaat
     5521 tatcggcggc ggtgatgtcg cgatggacgt agccagcacg ctgaaagttc tcggctgtca
     5581 ggcggtaact tgcgtagcgc gtgaagagtt agatgagttt ccggcaagcg aaaaagagtt
     5641 taccagcgcc cgggagctgg gcgtttcgat catcgatgga ttcacgccag tagccgtgga
     5701 aggcaataaa gtcacgttta agcatgtacg gttatcgggc gaactgacga tggcggcaga
     5761 taaaattatt ctcgccgtcg gtcagcatgc cagactagat gcctttgcgg agttagagcc
     5821 gcagcgtaac accatcaaaa cacaaaatta ccagacccgc gacccgcaag tctttgctgc
     5881 tggcgatatt gttgagggag acaaaaccgt ggtctatgcc gtgaaaaccg ggaaagaagc
     5941 cgccgaggcg attcatcact atttagaggg agcttgctca tgttaacgaa agatctttcg
     6001 attacttttt gcggcgtgaa gtttcccaac ccgttctgcc tctcttcttc gccggtaggc
     6061 aactgctatg agatgtgtgc caaagcctac gacacaggtt ggggcggtgt ggtgtttaaa
     6121 acgatcggct tttttatcgc caacgaagtc tcgccgcgtt ttgatcatct ggtgaaagaa
     6181 gataccggtt ttatcggctt caaaaatatg gagcagattg ccgagcatcc gctggaggag
     6241 aatctggccg ccctgcgtcg gctgaaagaa gattacccgg acaaagtatt gatcgcttcg
     6301 atcatggggg aaaatgagca gcaatgggag gagctggcgc gcctggtgca agaagctggc
     6361 gcggatatga tcgagtgtaa cttctcctgt ccgcaaatga cttctcatgc gatgggtagc
     6421 gatgtcgggc aaagcccgga gctggtagaa aaatattgtc gggcagtgaa acggggttcc
     6481 acgctgccaa tgctggcgaa gatgacgccg aatatcggtg atatgtgcga agtggcgctg
     6541 gcggcgaagc gcggcggcgc agatggcatt gcggcgatta acaccgttaa atccatcacc
     6601 aatatcgatc ttaatcagaa aatcggtatg ccgatcgtta acggaaaatc aagtatttcc
     6661 ggatattccg gtaaagcggt aaaaccgatc gccctgcgct tcattcagca aatgcgcacc
     6721 catccagaac tgcgcgattt cccaatcagc ggtatcggcg gcattgaaac ctgggaggat
     6781 gcggcggagt ttttattgct cggcgcagca acgttacagg tcaccaccgg cattatgcag
     6841 tacggctatc gcatcgtaga agatatggcg agcgggttat cgcattatct cgccgatcag
     6901 ggatttgatt cgttgcagga gatggtaggt ctggcgaata acaatattgt cccggcggaa
     6961 gatttagacc gcagttatat tgtctatccc cgtatcaatc ttgacaaatg tgttggctgt
     7021 ggacgctgtt atatttcctg ttacgacggc ggtcaccagg cgatggaatg gagcgagaaa
     7081 acccgcacac cgcattgtaa taccgagaaa tgtgtgggtt gtctgctttg tggtcacgtc
     7141 tgcccggtgg gttgtattga gctcggggaa gtgaagttta agaaaggcga gaaagaacac
     7201 ccggtaacgt tgtagaaccc gcgtcacatc cggcatttgg ttcctgttgc cggatgcggc
     7261 gtgaacgcct tatccggcct acaaaaccac acagatcatg atgttatgtg cgtttatgac
     7321 cgaatgcgga ccacattcac atcatttctt acgcgcgtat ttcagtgaat ccagcgctac
     7381 ggcgaagata ataatcgccc ctttgatgat gtactgccag tatgggttta cgccgatata
     7441 cgtcaggcca tagttgatga cggtaaaaat aatcaccccg gtcaccacgc caatcaccgt
     7501 ccccacaccg ccgctgaacg atacaccgcc taccacgcac gccgcgatag catccagctc
     7561 atacataaag ccgaggttgt tggtggcaga gccgatacgt ccggcttcta acatcccgcc
     7621 aaaggcatag aacacgccag acaacgcgta gatcatcagc aggttcaggc cgacgttgac
     7681 accagatact tttgccgctt ccgggttacc gccaatggca aaaatgttct taccgaagcg
     7741 ggttttgttc cacaacaccc agacgaacgc caccgcaatc aacgcgtaga aggtgatgta
     7801 agagagacgg aaactcccca gcgcgacaaa gccctgagca aaggtagaga agccactgtc
     7861 aaaaccagaa attggcgacg ccccgacaaa gtcgtaatag agcgagttga tgccatagac
     7921 gatgatcatc gtgccgagcg tggtaatgaa cggcgtcacg ttgagataag cgataatcag
     7981 accgttgatc aaaccgatca ccgcaccaat ggcacagaca atcagaataa ccagcgcaat
     8041 cggcatcgtc gccatttccg ggaacacttt gttggcgtta tccatggact gcaataatgt
     8101 cgcagccacc actgccgcca gccctacctg acgaccagca gaaagatcgg tcccctgggt
     8161 gacaattaac cctgccacac cgagcgcgat aataatacgc accgatgact gggtgagaat
     8221 attacttaag ttcaacagac ttaaaaatgt tgggtcctgg aaaataataa tcgccagcaa
     8281 aactaaaaga acgacgtaaa taccgccctc tttcaggtaa gtaagaaaac ttttcttatt
     8341 taacgcactc atgggaagcc cctaatctta aaggtgcaaa gacgcaagac gcagaatttc
     8401 gttttgcgtt gttgtttttg tatcgacaat tccggaaacg agaccattgc tcatgaccag
     8461 aatacggtct gttatcccta acaactcagg catttcagag gagataataa taatcccctt
     8521 gcctttcttc gccagttcgg caattaactg ataaatttca aacttcgccc cgacatcaat
     8581 accgcgcgtc ggttcatcga gcattaatat ttctggttgc gttagtagcc agcgaccaat
     8641 aatcaccttt tgctgattac caccggagag cgaaccaatt tgcgtccgat gacccggcgt
     8701 ttttacccgc atcgaatcaa tcacccactg ggtatcgctt ttcatccgcg agttatccag
     8761 taaaccaact ttatttttgt agttgcgaat attggaaatt aacgagttaa aaccaatatc
     8821 cagataggca taaattcccg ttgagcggcg ctcctcagtt accagtgcaa atccgtggtt
     8881 tatggcttcg ttggcattat ggttattgat ctgtttgccg tgcaacgtaa tggtgccagc
     8941 cgatttctcg cgaataccaa ataacgtctc aacaatatcg gtacgtttcg cccccaccag
     9001 cccggcaata ccgaggatct cccctttatg cagatcaaac gagacatcgc gaatcgacgg
     9061 ctggcgcagt gacgttaggt tacgcacctc gaggatgact tcgcccggct tgttttcttt
     9121 gtcaggaaaa cgctgattaa gagaacgccc aaccatcatg gcgatgatct tgtccatcgt
     9181 cagtcctgcc agcggttcgg tggcgatcca ctgaccgtcg cgcaatacgg taacttcatc
     9241 acataactgg aagatttctt ccattttatg cgagatataa acaataccgc agccgcgctc
     9301 ttttaattta cgaataatag tgaacagatg attgacctct ttttcggtta acgaagaagt
     9361 cggttcatcc ataatcacaa ttttcgcgtt ataggaaaac gctttggcga tttcgatcat
     9421 ctgcatttgc gaaacggata atgtgccgac gcgcgcacgc ggatcgatat caatatccag
     9481 ttcatcaaaa attgctttgg tttcgcggta cattttgtcc tgatcgacaa acatgccttt
     9541 ggtgggatat cgccccagcc acatgttatc catcaccgaa cgttgtaata ccaggtttaa
     9601 ctcctggtgt accatcgaaa taccattttc cagtgcctct ttggcggaat ggaaatcgat
     9661 ctctttaccc tggaataaaa tggtgccgga gtctttttga taaataccaa acaggcattt
     9721 taataatgtc gattttcctg caccgttttc ccccattaat gcatggatag aatgtggccg
     9781 gacttttaaa ttaacgttat caagtgcctt aacaccagga aaggacttgt tgataccgct
     9841 catttccaac aagtattccc cggaagacgg agtcgttgag ctgaccatat aattttacct
     9901 tgttggccat acaataaggg cgcagtaaac gactgcgccc aatcagtctt atttcttgct
     9961 gaattcagcc aggttgtctt tatctacgcc aacataaggt acgcggacca ctttgttgtc
    10021 gattttccag ttggtgccat cagccgcacc tttgccatcg gccaggtttt tcgccagatc
    10081 aaaggtcgct ttcgcctggt tgttagcatc gttcagtact gtgcccgcca gtgcaccgga
    10141 tttcaccagc gccagcgctt ctggcagcgc atcgacgcca aacaccggaa tgctggactt
    10201 gttgtgtgct ttcaatgctt ctaccgcgcc cattgccatc gcatcgttgt tggcgataac
    10261 cacttcgatt ttgttggcgt tcgggccaga cagccaggcg tccatcttat ctttcgcctg
    10321 agcggtgtcc cacattgcgg tatctaactg taactgttca gttttgatgc ctttatcgtt
    10381 caattcttta atcacgtaag tggtacgtgc ttctgcatcc ggatggcccg gttcaccttt
    10441 cagcagtacg aactgaatct gaccgtcttt gttcagatcc caaccctgat tcgccgccca
    10501 gtgtttagca atcaaatcgc cctgaataat gccggactct ttggagtcag tgccaacgta
    10561 gtaggctttg tcgtagctat ccagcgcctt acgagacggt tctttgttga agaaaaccac
    10621 cggcacgttt tgcccacgcg ctttctcaat caccgtaccc gcagctgccg ggtcaaccag
    10681 gttgattgcc agtgccttca cccctttcgc cagcaatacg tcgatctgat cgttctgctt
    10741 ggactggtca ttctgagaat cattcatcag cagctgaaca tctggcgcgg ctttcgcatc
    10801 ttgctcaata gccttgcgca ctacagacat aaagttatcg tcgtacttat agattgttac
    10861 accaatgcga gtatcagcag cgtgtgcagc ggcaccgaat aacatgctgg ccatcacagc
    10921 agacagggtt aacaccttct tattcatggt atctccggtt tttcttatgc agggtagtgc
    10981 ttgagataaa tgctcggcgg ggcagtagag ttaatgaagt gttactgaac gccgaagctc
    11041 acttttttaa attcgttctt ccatgcccgg taacgctcca gaaaacggct ttaattgttg
    11101 ttatgacgct attacctcgg tgaaagtgat tattcactgt tacatacggg ttacaacgtt
    11161 aaaacggtgc aatcatagct atcacattgt taagatactg tgaaatcact cacagattga
    11221 aagcggttac atcgcctgat ttgttgagtt agtgatcgcc gccgcattct ggcgcgttgc
    11281 gacagaatgg cgacgcacta acgtcggcat aaaacagtgg ctggcacgag gatcaatatt
    11341 gcctgctgcc ccctgcaagg ccagttcggt ggctaattta gccattgaag caatgggata
    11401 acgcacggtc gttaattgcg ggtcggtgta acgggcgatg ggaatatcat cgaaaccgat
    11461 gattgagaga tgtaacggaa tcgcaatgcc attatctttt aatgctgtca gtgcgccagc
    11521 ggccatattg tcgttataag caaatatagc ggtaagttgt agattgcgcc ccagcagttc
    11581 aaccatcgcc gcctcaccgc ccggcatgtc cggcgtacca gtgccaatcc agctttccgg
    11641 cggaataata tcctgctctt tcaacgcact catccagcct gctttacgca tggcgtcatc
    11701 ttcaatgccg tgactggaag aaagataacc aatacgttga tgaccgttat tcagcaacat
    11761 gcgcgtcgcc attcgggcac cgctgagatt atccaggcaa acgcaacgat gggcgtaccc
    11821 cggcacaacg cggttgatta acaccatacc gggaatgtta tccataaatt gcgccagttc
    11881 atcgtcactc aatgcttttg agtgaacaat caacgcatta caacgctggc gaattaacac
    11941 ctcaatggcg tgacgctctt tttccgcttc atgatagcta ttgccgatta gcacgtattt
    12001 ctgatgctgc tgagcgacta gatccaccgc ttttaccagc gcgccgaaaa acgcatcaga
    12061 aacgtccatc accaccacgc caatggtgtc gctaacctga gttgccagcg cctgggcatt
    12121 ggcgtttggc cgataatcca gctcactcac ggctttcatt actgcttcac gcgtgtcggc
    12181 actgaccagc gtgctgttat tgagcacccg ggaaaccgtt gccacagaga cgccagccta
    12241 acgcgctaca tcacgaatgg tgatcatatt cactacctgt tatgagattg cagtaaatga
    12301 ctgcttgctg gcggctattt tgtcagcact gaatacagga cttcgtgaat cgagtcacag
    12361 caatggaaac ggttacagcc gttttgttaa cgaatgtgat ccagatcgtt atcttgatgt
    12421 tttagatatt gccgttccgg cagcacgcaa agttaactga cgccagagcc attccaccgg
    12481 cccctggcgg aagtaacgca gccagataac agagaagagg atattcgcca gccataccgg
    12541 aataacaaac gccagcagct ccaggcggtc aaaatgcata aacaaaccga ggtggtaaaa
    12601 aagcatggta caaatcagcg tttgcaatag atagttggtc agcgccatcc gtccgacgca
    12661 ggcgatcgca agcaccagct taaagcggct caattgcggc cagaagccat aaaacagcga
    12721 cgcatagcca atcgcctgaa acggcgcact cagttcccgc ggcatttgaa gtaagaaggc
    12781 acaccagcga tacgcccagt ccagctgcca ttgcagggcg atggcaggaa ggttaatggt
    12841 caccccaatc gccaccagca caaaaccagt acgacgatag tgacgtaagc tgaactgccc
    12901 tttcagccag ccgctgcgca tcagcgccgc accaatgagc atcatccctg ccagttgcca
    12961 gccatactgt gcgcccagtg ccagtaaact gttgccaaca ccatcggcac gattactgat
    13021 cgcttcaacg ccgccgtgaa gcttccagta tttttcatac aaaatagccg atgcatccgg
    13081 cgtccaggtg cggctggtct ggctgtcgga aatcagcccc aataacagca aaacgccaag
    13141 ccccaccaga taaagcatga cgcctgtatt aaacaggctt tttaccgatg gcgcatcgcg
    13201 caccagccgc cagcagatta agcccaccag cccgtaagcc agcagaatat cgccgtccca
    13261 gaacaataaa ccgtgaataa aacccagcaa gactaacagc gttaaccgcg actggatcca
    13321 gcgtctgcca cggggcagca acatttgcag gcccgcacca aacagcagcg caaaaagcgt
    13381 gaggaatttc acctggccga tgagatcgag aaatgcccag gtccatgcat cctgcggcgt
    13441 aatagcgccg taccaggcgg gattgagata agccgccttt ggtagcccaa aggcgctgat
    13501 gtttaatagc aggatcccca gaatggcgac gccgcgaaca aaatcgagcg ttacgttgcg
    13561 ctccatggtt cctgcctttt aatcagttgt gatgacgcac agcgcgcaga aactcgtggc
    13621 gcgtattctg actggatttg aacaatccac caagagaggt cgttgttgtg gcactggttg
    13681 catcgcggat gccacgcgct ttcacgcagt aatgcaccgc atcaatggag acagccacgt
    13741 tattggtgcc cagcagcgtt tgtagcgcaa taagaatttg ctgcgtcaga cgttcctgca
    13801 cctgcggacg ctgggcaaag aactgcacga tacggttaat ttttgacaga ccgatcaccg
    13861 aatctttcgg gatataagcc accgtcgctt tgccatcgat ggtaacaaaa tggtgttcac
    13921 aggtactggt cagagtgata tcgcgcacgg tgaccatttc atcgaccttc attttgtttt
    13981 caatgagggt gattttcggg aagttggcgt aatccagacc ggagaaaatt tcatcgacat
    14041 acattttagc gatgcgatgc ggcgtttcca tcaaactgtc atcagccagg tcgagattca
    14101 gcagctgcat gatttcggtc atatgaccag caataaggct tttgcgcgtt tcgttatcca
    14161 tttcatgcac gggcgggcgc agcggtgttt ccagtcctcg cgcaactaac gcttcatgaa
    14221 ccagggccgc ttctttactg agtgatggca tttatgattt ctcctgcagg tgtgacgcct
    14281 ccgccctgcg tgggggcaaa gttattaagc tgatttacag cctgattatt gtgcgtgagg
    14341 cggcgcacat aatccagtat tcacagcgat aattattgta attgccgctg actttcatca
    14401 gcagatgtta aaacatcgtt atgcaaatac ggaagtgaaa gttactcaca gcacattgaa
    14461 taaacggtat gatgaagaaa ttgcaaacaa cacaacaagg agccacgcat ggaaatgctc
    14521 gaagagcacc gctgttttga aggctggcag caacgctggc gacacgactc cagtacctta
    14581 aactgcccga tgacgttcag tatctttctc ccaccccctc gtgatcacac cccgccacca
    14641 gtgctgtact ggctttccgg attaacctgc aatgacgaga acttcaccac caaggcgggt
    14701 gcccagcgcg tggcggcaga actggggatt gtactggtga tgccagacac cagcccgcgc
    14761 ggcgaaaagg ttgccaacga cgatggctac gatttaggcc agggcgcagg cttttatctt
    14821 aatgccacgc aaccgccgtg ggcgacgcat taccggatgt atgattatct gcgcgatgaa
    14881 ttaccggcgc tggttcagtc gcaatttaat gtcagcgacc gctgcgccat tagcggtcac
    14941 tcaatgggtg gtcacggtgc gctgattatg gcgctgaaaa atccgggtaa atacaccagc
    15001 gtttcggcct ttgcgccaat tgtgaatccg tgcagcgtcc cgtggggaat caaagcgttt
    15061 agcacctatt taggtgagga caaaaatgca tggctggaat gggatagttg cgcgctgatg
    15121 tatgccagta acgcgcagga tgcgatcccg acgcttatcg atcagggcga taatgatcag
    15181 tttcttgccg accagttgca acctgcggta ttggcagaag ccgcgcgcca gaaagcgtgg
    15241 ccgatgacgc tgcgtattca gccgggatat gatcacagtt actacttcat cgcctctttt
    15301 atagaggatc acctgcgctt ccatgcgcag tatttactga agtgaaagtc cgcccggttc
    15361 gccgggcatc ttctcatcag aagcgataat ccactgccat aaagtaacga cgtccgtctt
    15421 cgttatagct gtagtcgtca cgactgagat ccttgtcgcc aaggttcagc acgcctgcgc
    15481 gcagtttgac gtctttagtc acctgccagg ccgcgccggt attccagatg gtataaccgc
    15541 ccggtgtttt agccgtcgcg ctgtcggcgc gtttctgccc ggtatagtga ccagaaacat
    15601 agaatgacca gtcttccagc gccagcggtt tccagtccag cgtaccgtta gcagtatgga
    15661 acggcagatc ggatagcggt ttgttttcgc cgttgctgac atcacgacca tcgttgtagg
    15721 tgtagttgat cgacagtttc cattcatcgt tgaacggaat tttcagttcg gtttccacgc
    15781 cctgaatacg agctttgtta acgttgtagt agctaaatac cggtatgcgc cgtccgttag
    15841 cgcccgtctc aaaaccaaca aagttttggt agcccggtgc agcgttgacg tcagacgtac
    15901 ggctgatgct gatacgatct ttcacatcgt tacggaaaac ggtaacgctg gattcaaccc
    15961 cttccagcca gccttcttca cccatgtagt aaagccccag ctcccaactt tcgctggttt
    16021 ctggtttcag atccgggcta cccacaatct tacatgcgcc acggcaggaa ttgctcgtcc
    16081 agtcagggct aagttgcaac agagaaggtg ctttaaatgc cgtcgcccag ccccctttca
    16141 ccgttacggt gtcggtggcg ttataaacca ggtaggcacg cggactccag tgttcaccgt
    16201 aggtttcgtg atcgtccata cgcacgccgg tcgtcagcgc cagcggctcg aagatccgcc
    16261 attcatcttc cacaaacagc gcgtactggc tggcagacgt tttggagctg gttcccccgg
    16321 tcaggttcac cgcatcgcta agtttgtcgt gacgccattc accgccaacc gtgagaaact
    16381 gattaatcgc cgtcagcggc aacgtgtatt tgccgtcgac cgtattgctt tcggaagtta
    16441 tcgggctgct gttgccaggg tttttgttct cgactttctc accgtagtat ttcagttcgc
    16501 tggtgccgta atcccaacgc ccattatggc tgacggagta gttctggcgt tccaggcggt
    16561 ttttgtccag cgagtcggaa tcacgatcct gacggtcgaa accgtatccg gcagtaaaat
    16621 cgtgattttg attcggtgtc caggcaaatt cgacattgcc gtcgcggctg gagaatcctt
    16681 caatacgcgg cgtttctccg gtatcggtgg tcgttgagtt ttgcgggtca tccttttcac
    16741 gttttgccag gctgccgtaa gctttcattc ccagcacacc atcaattaat ggtccactgg
    16801 taaagaactg accgttatag gtatcaccgc gatcgcgatg ttcctgaatg gtggtatcga
    16861 cggtaacggt acccgaccat ttctgaccga tttttttggt gatgatattc actacaccgc
    16921 cgagcgcatc ggaaccgtac agcgacgaca tcgggccacg gaccacttca atacgttcga
    16981 tggaatcgac cgggatccag ttcagatcga aatcattgtg gcggaagacg gcattgcggg
    17041 agttcacgcg tttaccgtcg acgagaatca gggtatagct gctgtccaga ccacgaatac
    17101 taacgccctt acggttatcc ccttcgttcg tcagttgtac gccaggcact tctttgagga
    17161 catccttcag attctgtacc ggttttcgct gcagatcttc ctgggtaatg acgctgatac
    17221 tggcgggtgc atctttaagg ttttgttcaa cggaagatgc agtgacaacc atcgtttcgc
    17281 catcgtcatc gaccgctaac acaggccata cacaagaaat agcggacaaa cacagcccga
    17341 cccgtacgaa agggttcaac ctaaacattc catatctcca tgaggtaact acgaaaataa
    17401 aatgggtatc gctcacatct tctttacgtc cccttgcgta cggcagcatc gcggtggact
    17461 tatttgtatg caggtgatca tcccagaaag ccggaccagc ctctgatttg tcagcctttt
    17521 ttgattgtag cgtaacgata atgcaaacga taacaattat caatccaaat gttaaatttt
    17581 atatccgcat gatttgtcag ggaaatggaa ttgaaaaagc cctctcggtt gagagggctt
    17641 agcaaggaag ggaggaaact tatttcttat cgttctgcgg gaacttcatt tcgctgtagc
    17701 gtacgaagtg agttccttta atcagcttgt agccgaacca aataatcagg aacagcggga
    17761 taccaatata cgttgccgct acgccgcccc agtcaatggt atctttcagg aacgcttcgt
    17821 agttctggcc caaagtgata atcagacaca gaatgaatgc gaagatcggc cccagtggga
    17881 agaaacctga acggtacggc agatcgttaa tgtcgtgtcc ctgcaatacg taaccgcgac
    17941 ggaagcgata gtggctaatg gcaatcccca gccaggcgat aaaacccgtc atcccggagg
    18001 tgttcagcag ccacaggtat accgtctggt tgccaaacat ggaggtcagg aagcacaaac
    18061 cggcaatcac cgtcgtcgca tacagcgcat tacgcggcac gccaccacgc gacaatttag
    18121 cgaaaatgcg cggcgcttta ccgtcacacg ccagggtgta cagcatacga gtagacgcat
    18181 acataccgga gttacctgct gacagcaccg ccgtcagaat aactgcgttc atcaccgccg
    18241 ccgcagagag cagacccgcg tgctggaaca ccaaggtgaa cggactgacg ctgatgtctt
    18301 taacatcgtt acgcagcagg ctcggatcgg tatacggaat aatcaggctg ataatcagga
    18361 tcgcgaacac atagaacaac aggattcgcc agaatacctg acgtaccgcg cgtggaatgt
    18421 ttttcgccgg atcttcggac tcgcctgcag caataccgat cagctcggtt ccctggaaag
    18481 agaagccgac aatcatagct acgccgatca tcgccgcaaa accaccagca aacggcgctt
    18541 cgccgattgt ccagttgctc cagcccgcag gctgcgcgcc tttgaagata ccgataatca
    18601 tcagcacgcc aacgatgata aagacaataa ctgtcgtgac tttgatcagt gaaaaccagt
    18661 attccgcttc accaaagcca cgaactgaga tgtagttcag caggaagata acgccgagga
    18721 acaacgcact ccagatccag cccggtgtat ccgggaacca ccagctcatg accagctgtg
    18781 ctgcaaccaa gtcaacggca atagtcaccg cccagttgta ccagtagttc cagcccagcg
    18841 cgaagccaaa gccttcttca acatagttct gaccgtaagt ggcaaacgaa ccggaaaccg
    18901 gcatatatgc agccaattca ccgagactgg tcatcaggaa gtaaaccatc aggccaatca
    18961 gcatatacga gagcaatgcc ccgcccggac ctgcctgaga aatcgttgcg ccagaggcaa
    19021 caaaaagacc tgtaccgatg gaaccgccaa tggcaatcat cgtcaggtga cgcgccttta
    19081 attcacggcg taagcccggc gcttctgtag ttttagtttc ggaacccata caaaaatgct
    19141 atccatctta aaaatgaggc gcgattgtag cagacgattg gcaatccttc cggcagaaat
    19201 acacggttat aagacacctt catgatcgcc cagggattat aagtaaagca gccaatctct
    19261 tttctggcga atgcatgctg agggcgctgc gtcacgaaat cgacacagca ccagcatgtt
    19321 cttgtacagc accttaacgc ggtacattcg cgggatcgca atagtccaga aagcgccgta
    19381 gcgcgttgga aaggtgtttt tgccgatgat gtatacgcca caacgtacgc atcaggcgcg
    19441 ggagagggac cgcaacttca cttaatgtgc ctgcctgcaa ttgatcttca atcacacgtc
    19501 gcgacaggca actaattccc aacccatgac gcaccgcatg tttgattgcc tcggagttac
    19561 ctaattccat cgccatctca aacttcggta aatgtgacag caacagataa tcgacaatct
    19621 cccgcgtgcc ggaaccgcgt tcacgcagga tccacggcgc agcggccagc tgttctaagg
    19681 tgaccggacc acgagccaac ggcgaagtcg gcgcggcgaa aaccaccagc tcgtcttcca
    19741 gccacggttc agaaatgatt tcagtgctgt ggcacggtcc ttcaataaag ccaatatcaa
    19801 cgcggaaatc cagcaccgct tgcatcacgt cctggctgtt cccaacgcta agttcaatcg
    19861 gcaactgcgg ataatcatgg cgataacggg cgataactgc aggcagaatg tagttaccga
    19921 tggtactact ggcatagata cgaatcgcgc cgttgtcttc gcgaaacagt tgttcgattt
    19981 caaccgcctg ttcaagcaat gccaacgcac gcggatagag cagccgcccg tgttcattaa
    20041 caaccagtct tttccccacg cgatcaaaca gttgcacgcc aagctgccct tccaggtcgg
    20101 tcaaggctgc gctcactgct gattgcgaca acgccagcat caccgacgcc tgggtggttg
    20161 atccactttt caatacttct gcaaaaactt ccaactgccg gagggtgatg tgcatagtcg
    20221 cttaccactt ataaagatta attataaata tataatcaat tttattttta aaccagttag
    20281 tcgtaacctt atgcccggtt aaaggagagg gttatgacga atatcacctt acagaaacaa
    20341 catcgtacac tgtggcattt tattccgggg ttagccctga gtgcagttat caccggggtc
    20401 gccctgtggg gtggttccat tcccgcggtt gcgggtgccg ggtttagtgc cctcaccctc
    20461 gcaatcttgt tggggatggt tttaggcaac accatctatc cgcacatctg gaaaagctgt
    20521 gacggtggtg tgctgtttgc caagcaatat ttattacgtc tgggtatcat tctttatggc
    20581 ttccgtctga cgttctcgca aattgccgat gtcggtatca gtgggatcat cattgacgtg
    20641 ttgacgctgt ccagtacctt cctgctggct tgcttcctgg ggcagaaagt gtttggtctg
    20701 gataagcaca ccagctggtt gatcggtgcc ggtagcagta tctgtggtgc tgccgcggta
    20761 ctggcgactg agtcggtagt gaaagcggaa gccagtaaag taaccgtggc tgttgcaacc
    20821 gttgttatct tcgggaccgt cgcgattttc ctctacccgg cgatatatcc gctgatgtct
    20881 caatggttta gtccggaaac tttcggtatc tacattggtt ctactgtgca cgaagtggcg
    20941 caggtggtgg cggcaggtca tgccatcagc ccggatgcgg aaaacgcagc agttatttcc
    21001 aaaatgctgc gcgtgatgat gctggctccg ttcctcatcc tgctggcggc gcgtgttaaa
    21061 cagctgtctg gggcgaacag cggcgagaaa agcaaaatca ctattccgtg gtttgccatc
    21121 ttgttcatcg tcgttgccat ctttaactcg ttccacctgt taccgcagag cgtggtgaac
    21181 atgctggtaa cgctggatac cttcctgctg gcaatggcga tggcggcgct gggtctgacc
    21241 actcacgtca gcgcgctgaa aaaagctggg gcgaaacctc tgctgatggc actggtactg
    21301 tttgcctggc tgattgttgg tggtggtgcg attaactatg tgattcaaag cgtcatcgca
    21361 taaaccacta catcttgctc ctgttaaccc gctatcatta ccgttttcct ccagcgggtt
    21421 taacaggagt cctcgcatga aatacattgg agcgcacgtt agtgctgctg gcggtctggc
    21481 aaatgccgca attcgcgccg tcgaaatcga cgcaaccgcg tttgccttgt tcaccaaaaa
    21541 ccaacgtcag tggcgtgccg caccgctcac gacgcaaacc atcgatgaat tcaaagccgc
    21601 ctgtgaaaaa tatcactaca catcggcgca aattcttccc cacgacagtt atctgattaa
    21661 cctcggacat ccggtcactg aagctctgga aaaatcgcgc gatgccttta tagatgaaat
    21721 gcagcgttgc gaacaactgg ggctttcttt gctcaacttc caccctggca gccatctgat
    21781 gcagatttca gaagaggatt gccttgcgcg tattgccgaa tccatcaaca ttgcgctgga
    21841 taaaactcaa ggtgtaacag cggtgattga aaacaccgcc ggtcagggca gtaacttagg
    21901 gtttaaattc gaacatctcg cggcgattat cgacggcgtg gaagataaat tccgcgtcgg
    21961 cgtctgcatt gatacctgcc atgctttcgc tgccgggtat gatttgcgta ctccagccga
    22021 atgcgaaaaa acgttcgctg attttgcccg tattgtcggc tttaagtatc tgcgcgggat
    22081 gcaccttaac gatgcgaaaa gtacctttgg cagccgcgtt gaccgccatc atagcctcgg
    22141 tgaaggcaat atcggtcatg atgcgttccg ctggatcatg caagacgacc gtttcgacgg
    22201 cattccgctg atccttgaaa ccatcaaccc ggatatctgg gcagaagaga tcgcctggct
    22261 gaaagcgcaa caaactgaaa aagcggtggc ctgatcgatg aacgacaggg aaaagcagat
    22321 tcttaaaatt ttacggcgta acccgctgat tcaacagaat gaaatcgccg atattctgca
    22381 aatcagccgc tcacgtgttg ccgctcatat tatggatttg atgcgcaagg gtttgattaa
    22441 aggcaaagga tatatcctta ccgagcagga ttactgtgtg gttgtgggtg caatcaatat
    22501 ggatattcgc ggaatggcgg atatccgtta cccgcaagca gcctctcatc caggttctgt
    22561 tcactgttcc gcaggcggcg tcggacgtaa tatcgcgcat aacctggctc tgcttggtcg
    22621 ggatgtgcat ttaatatcag ctattggcaa cgatttttac ggcgagacat tactggaaga
    22681 aacgcgccgc gcgggcgtga acgtttcaaa ctgcattcgt ctgcatggac acagcactgc
    22741 aacttatctc gctatcgcta ataagcagga agaaactatc ctggcaatca atgacacaca
    22801 tattctgcaa cagctaacgc ctcaactgct caatacttca cgggacttga ttcgccatgc
    22861 tggtgtggtg ctggctgatt gtaatctcac acctgaagcc ctggagtggg tctttaccat
    22921 tgctgatgaa attccgatgt ttgtcgatac cgtttccgaa tttaaagcga acaaagtaaa
    22981 aaactggtac agccggatcc acactctgaa accaacacaa aatgaactgg aaattttgtg
    23041 ggggcaaccg attaaagacg acaacgatcg catcagagct gtaaattcgc tgcatcaaca
    23101 aggtgttaaa cgcatttttg tttatctgaa agatgaatct gtattttgca gcgataagga
    23161 tggagaacaa tttttgctga ccgcgccagc gcatacgaca gtagacagtt ttggtgctga
    23221 cgatggtttt atggcgggcc tggtatatag ctttcttgaa ggatacagtt tccgcgacag
    23281 cgcccgtttt gcggtagcct gcgcggcaat ttcgcgcgcc agcggcagct taaacaaccc
    23341 taccctgtct gccgataacg cgctttcatt agtgccaatg gtgtaacaat gttgccggat
    23401 tcgacgcttg accgcatctt atccggctca taaattgcgc actacgctaa accaataaag
    23461 aatcctgcaa tagtagcact catcaggtta gaaagtgtcg ccgccgccag cgcgcgtaaa
    23521 ccaagttggg cgatttccgg cgcacggtgt ggcgcaaccg cagaaaacgc ccccaccacc
    23581 accccgatag aaccaaagtt agcgaaaccg cacaacgcga aagaaataat cgccacggtt
    23641 ttagcatcca gagtgccacc cgtttgcaga tatggcgaga aattgagata agcgacaaat
    23701 tcattgatcg ccagcttctg cccaatcaaa ctcccggcaa gatttgcatc actccagtca
    23761 acccccatca cccacgccaa tggggccaac aggtaaccta aaatcgactc cagcgaagca
    23821 tgttcaaaac caaaccagcc gccaacaccg ccgataatac cgttaatcaa cgcaattatt
    23881 gcaacaaatg ccataacaac tgtcgctaca ccggcggcga ttttcagccc ggtcattgcc
    23941 cctgtcgcag cggcttcaat aatgcttttt ggcggtgttt cggtgaaaga gaggttatta
    24001 aaagaaacct gcgaagattc cgtagccggg cttaacaggc gggcaaacaa gatcccgcca
    24061 gggatcgcca ttaacgatgc cgccagcaaa tattccacag gtacgcccag tgcggcgtaa
    24121 ccaatcattg tcgaaccagc aatcgaggcc atgccactac aaatcgctgt aaataattca
    24181 ttgcgattca gacgatcgat aaagggtttg acgatcgccg gaatttcgtt ttgcccgagg
    24241 aaaatggtgg tgaccgcgac gaatgactcg atcttgctga tatttaaagc tttctggaag
    24301 ataccgccga gaatgcgaat taagatcccc atcacaccga tgtagtagag aatactcacc
    24361 agtgcagtga cgaagataat tgccggtaat accctgaaac caaagataaa tcctgcgcca
    24421 tcaaataagg tatccatttt cggtccgacc agagaaccga agataaatgc gctacctgcg
    24481 tcgctgtacg ccatcacttt gtggacgcca aaagcgactt tttctgcgac ccaacgccct
    24541 ggcggtaacc aaagcataat cccgccaatc acgacctgta acactaacgc cgcgccaacg
    24601 gtacgcaggc tgatcttctt cttgtttact gacagtaaaa acgcaattgc cagcagtacc
    24661 accattccca gaacacttct catgacatcc atagtgatta tctcttcatg tcattttggg
    24721 cgacggcttt ctgcccatca gaaatagccg ggcctgcatc ttgcgatact gtcccggctt
    24781 gttatcaaca cttaatgggt ttttatgtag ccgcgtacgc actcttcgac taatccccag
    24841 aaccagtcgg tatcaatagt gatgccgact ttagtattgg cgggcttgcc aagaacgccc
    24901 agctcgtcgc agacggtacg cccatagcaa gggccactgt tgacgtccac ttcgacgtac
    24961 atctcctggg ttttaatgcc atcagggtta atcagataac cgatgcaggt ggcgtcgtgc
    25021 accgggccgc cagccaggcc gtagttttcg aactgcgttt tgagagtgaa gttcatgatg
    25081 tcgctgaaca tctctccggc agggccgcct gccctttcca ttcgtgcaat tacgtccggg
    25141 gtgcaaacgg tctggttggt aagatcgagg cccatcatca ctaatggaac gccggaggtg
    25201 aacaccacgc gtgcggcttc cgggtcggca aagatattga actcggcaga tggggtgaag
    25261 ttgcctgtac cgtaagcgcc gcccattagc acaatttcac ggattttggg caggattgcg
    25321 ggttgcatac gcattgccac cgcgatattt gaaagcggac caaccggcac cagagtgata
    25381 tcgccatcgc ttgccataag ggtatcaatg atgtatttca ccgcgtgggt actttctgcc
    25441 tggcgggtca gcggctcgaa taccgggcca tccagtccgg tttcaccgtg aatattatcg
    25501 gcaacgattt gttgacgcat aatgggttgc ggcatccccg cataaaccgg aacattaatc
    25561 tccagtttct ggcaaacatt caggccatta attaatgttt tatcaagcgt ctgattaccc
    25621 gcaacaatgg tgatacctaa taaatcaatc gccggatgtt tcgccgccat cattatagca
    25681 atagcatcat catgacccgg atcacaatcc agaataattt ttctcttttc cattgtttat
    25741 ttcctctgtt tccagttgcg ttattttttc tacagcaacg aaatttcgat cgggccgatg
    25801 attgaatctt aacaacagcg tacgaatgct aaatatgaga aatctcatag cggataaaca
    25861 tcgtgaaaga aatccacaat aatgatctta agcagcaatt gatgagtgaa tccgcgttta
    25921 aggattgctt tttaacggat gtttcagccg atacgcggct gtttcatttt ttagcgcgtg
    25981 actacattgt gcaggaaggg caacagccgt cctggctgtt ttacctgacg cgaggccgcg
    26041 ccaggcttta cgccacgcta gctaatggtc gcgtgtcgct gatcgatttc tttgccgccc
    26101 cctgttttat tggcgagatt gagttaatcg acaaagacca tgaaccgcgt gcggtgcagg
    26161 ctattgaaga gtgttggtgc cttgcgctcc ctatgaaaca ttaccgcccg ctgttattaa
    26221 acgacacgct atttttacga aaactctgcg tcaccttaag tcataaaaat tatcgtaata
    26281 ttgtttcttt aactcagaat caatcatttc cgttagtgaa tcgcctggca gcatttatat
    26341 tactctcgca ggaaggtgat ctttatcacg aaaagcatac gcaagcggca gagtatttag
    26401 gcgtttctta tcgacatctt ttatatgttc tcgcgcaatt cattcacgac ggtttattaa
    26461 ttaaaagcaa gaaagggtat ctaattaaaa acagaaagca gttgtcagga ctggcgctgg
    26521 agatggaccc ggagaataaa ttctccggga tgatgcagta aaaattattg gcaatagcac
    26581 gattgccgga tgcaacgctt aataaggcgt acatccggca atgaaactca agctaaacca
    26641 ataaagaacc cggcaatggt cgcgctcatc aggttggaaa gcgtcgccgc cgccagcgcc
    26701 cgtaaaccaa gctgggcgat ttccggcgcg cgttttggcg aaatagccga aaacgctcca
    26761 acgacaacac cgatagaacc aaagtttgca aacccacaga gggcgaacga gataatggca
    26821 atggtcttga catccagcgt gccgctcgtt tgcagatagg gcgagaaatt gagataagca
    26881 acaaactcat taatcgccag tttctgccca atcaaacttc cggcaagatt tgcatcactc
    26941 cagtccaccc ccataatcca tgccaacggg gccagcacat agccgaaaat gctttccaga
    27001 gagacattgg caaaaccaaa ccagccgcca actccaccaa taatgccgtt gatcagcgca
    27061 atgatcgcta caaatgccat taccaccgtc gccacaccgg cggcgatttt cagcccggtc
    27121 attgcaccgt tcgccgcagc ttcaataatg ctttttggcg gtgtttcgct aaacgacaga
    27181 ttttcaaacg taacttgcga aggttccgtc gccggactaa gaatacgtgc aaacaggata
    27241 ccgccaggaa tagccatcag cgaagcggct aacaaatagt caatcggcac ccccattccg
    27301 gcatagccaa tcatcatcga acccgcaata gacgccatgc cgctacaaat agccgtaaat
    27361 agctcgttgc gattcatgcg atcaataaaa ggtttaacga tcgccgggat ctcattttgc
    27421 ccgaggaaaa tagtggtgac tgcgacaaaa gattcgattt tgctgatgtt gagggctttc
    27481 tgaaaaatac tgccaagaat acgaatcagc agccccatta cgccaatgta gtacagcaag
    27541 ctgataagcg cggtaacgaa aataatcgcc gggagtacgc gaaaggcgaa gataaagccc
    27601 gcaccgtcaa acagaacatc cattttggga ccgaccagcg aaccaaaaat aaaggcactt
    27661 cctgcgtcac tgtaggacat cactttatgg acgcctaatg ccgcctgctc tactgcccat
    27721 tttcccgggg gaaaatagag catgatgcca ccaatggcaa tttgcagcag cagcgcagcg
    27781 ccaaccgtgc gcaagctgat gctcttttta tttaccgaca acacaaaggc aattgccagc
    27841 aataccacca tccccgcaac acttctcatt atatccataa tgattttccc ttcatgccag
    27901 taaacccggc gtcagcgcca ggttttggta tgtttgatga gtacgggcga cggctttctg
    27961 cccgccagaa attaacccac gagacgctga tattctttgg caatttcgct cgccagaatg
    28021 gcgttattaa agaccagttg aatattggat ttcaggctat caccgccggt tagctccgca
    28081 acacgcgcca gcaggaacgg tgtactctct ttcccaataa caccctgttc ttcggcttca
    28141 gcaacggctt gatcaattgc tgcattgatg gtttcttctg gcatcgcaaa ctgttccggg
    28201 atcgggttcg ctaccaccag gccgccgttc aaaccggttt gccacttcac tgccatcgcg
    28261 cgagcaattt cgcgggcact gtcaagacga atgctgactt caaatgggct ggttcggcag
    28321 aaaaacgcag gcagcgcttt tgtctgataa ccaattaacg gtacaccgaa ggtttctaaa
    28381 tattctgttg ttaatcctaa atcgagaata gattttgccc ccgcacaaac aacagtgaca
    28441 ttggtatttg ccagttcctg taaatcggca gaaatatcga aggtatgttc cgctccgcga
    28501 tgcacaccac caatacctcc ggttgcaaaa actttaatcc cagccagtgc tgcaataatc
    28561 atggttgatg caacggtagt cgcgccattt ttccctgcag caacgacaaa aggtaaatca
    28621 cgacgactaa ctttcgtcac gttatgtcct tcacggccca gcaattcgat ttcttcttta
    28681 cttaagccca ctttcatcac accgccaata atggcgatag tggctggtac tgcgccctgt
    28741 ttacgaattg tttcttctac ttcaattgcg gtttgggcat tttgtgggaa tggcattccg
    28801 tgagaaatta tggtcgattc cagcgcaaca accggttttt tattttttaa agcatcctgt
    28861 acttcagcgg atatttgtaa taattcaggg gacagagtta attcagacat tctgtgttct
    28921 ccactaacga tctaacattg gcaattgata attcagggtt gttggtatat tcacaggcaa
    28981 gtgccattga cgaacatccc tgcgcaaatc gaacagaatc gataaacggc ataccgtcta
    29041 cccaacagga ggccagcccc gccatcatgg cgtcgcctgc cccagtaaca ttgatgacat
    29101 tcgttttaat aggggccgac cacccacttt caccgttgat atcactgtag taaacgccat
    29161 cccctcccat gctcagcacc aagcggttca ggccgtgttg atgaaaccag gcggcgacct
    29221 ttgcgacatc ttcacgtccg gaaagtgcaa ttccgctcaa cgtttcggct tcaagacggt
    29281 taggttttaa cgtatggatt ttgctcagat gatcgcggat tttgacgcat ttccatgccg
    29341 ataccggatc aataaataca ggcgtttccc cgctattttc cagaacccag accagcacct
    29401 cttcactaag gttgcaatcc gcgacaataa cctttgcacc gcgaataaac tcctgatgct
    29461 gggcaagaaa ttctgcagaa atacagtcac taatactcat atcatttatc gcgacaagca
    29521 tttctcctgt attatcgagc agtgataaat agctggatgt attctcaccc ggaacaatta
    29581 agcatttatc gacataaaca ccggattgat tagtttgcgc cagtaatgat tgcccataaa
    29641 aatcaccacc tactgcactt aaaagccagg catttttccc cagtaacgcc agattatgag
    29701 cgatattcct tcccacgcca cctggggtaa atttaatttt tccgggattc gaatccgcat
    29761 aattcaatgg atgatgtgaa tacccagcca catcaatatt cgccgaacca ataattacta
    29821 tgtagtcctt ttcgcgcata gcacgccccc tggcaataaa ttgccaacga agaaattggg
    29881 taaaatagcc attacagtca ataagactat tagagcacgt gttcagattt aaacacatgt
    29941 tcataataag catatttaac gtaatgtaaa tcgatcagaa aaaacgaaat cggccagata
    30001 tgaaggcggt cacaaagttt gtctatttat gcagcaacaa aagagaaagt tgtgatgtgt
    30061 aacacacaat atggcaggca agaaaaaggg cagagatgtt tctgccctcc agattgttga
    30121 caacgcgcgc tttgttcatc ctggatgtgg cgtaaacgcc ttatccgacc tacaaaagcg
    30181 caaaaaattc aatatattgc agggttcatg taggtctgat aagcatagcg catcaggcaa
    30241 ttttgcgttt gtcatcatcg gggcagagat gtctcatccc cgataatgaa gataaattat
    30301 gcgttttttt ctactatttg tgtttccggg cgtttcagga aggcataggc caaacccgcc
    30361 accagcgtac cggcaataat tgctaccagg taacccaata ccggcgtaat agcgccaggg
    30421 atcagcagaa caaacagacc accgtgtggt gccatcagtt tcgcaccaat cgccattgag
    30481 attgcgccag tcagcgcccc accaacgata cagcacggta gcacacgcat cggatcacga
    30541 gcagcaaacg gaattgcacc ttccgaaata aagcacagac caagtaccag agcggctttg
    30601 ccaccttcct gctgcgcttt gtcgaatttg cgacgcgcca ccattgttgc cagacccatt
    30661 gccagcggtg gcaccatacc tgccgccata atcgccgcca tcgggccata ggtttgagta
    30721 ctcagcagac ccacaccgaa tgcgtacgct gctttgttta ccggaccgcc catgtcagta
    30781 cacatcatgc caccgaggat cgcccccagc agaaccgcat tcgcagtccc catggtctgc
    30841 agccagtgag tcaacccatc gagaatgcca gcaaccggtt taccgattag gtagatcatc
    30901 gccagaccga ccaccagact ggaaattagc ggaatgatca ggatcggttt cagcgcctcc
    30961 atactctgtg gcagtttcaa ttgcgtactg attaacttcg caatgtaacc agccaggaag
    31021 ccagcaatta taccgccaat aaagccagaa ccggtgctga ccgccagcat accgccaatc
    31081 agacccggtg tgaggcccgg acgatcggca atggagaagg caatataacc tgccagtacc
    31141 ggcaccatca gcgcaaaggc tgaaccacca ccaatctgca tcagcgccgc tgccaacgta
    31201 cccggctctt taaacgcttc gataccaaaa gcaaaagaaa gcgcgataca cagaccacct
    31261 gcaacaacca tcggcagcat gtaagagacg cccgtcagca agtgacggta agcgcctgca
    31321 ctctctttct taccttcagt ggtcgctgtt tgagctttgc ccgctggttc atacggcgtt
    31381 gcttcagcaa ccgctttatc cagttcctgc gcagttttct tcagcgccag accggtagag
    31441 gtgcgataca tcggtttacc agcaaatttc gccagatcca cttcgatatc tgccgccaca
    31501 atcaccagat ccgctgctgc gacttcttca ggggtgattg cattgcccgc gccaacagaa
    31561 ccacgggttt caactttcac ccaccagcca cgttttttcg cttcggtttc aatggcttca
    31621 gccgccataa aggtgtgtgc tacgccagtc gggcaagcag tcaccgcaac tacgcgtttc
    31681 ggaccgctgg cagcaaccgg tgctgtcgca gcgaccggcg cagtgtaagg tttcgcatgg
    31741 cctttggctt cactcaggaa cagctcaggg tgcgcaactg cccgggaaat atcacccagc
    31801 cagacatttt taccgttcag cgcgctgtca ttcgggatgg aatcaccgag aacaatcgcc
    31861 atttctgcgt cgttcggatt gtcgatgatt tccagttttg cttttcgcgc cgccgcgccc
    31921 agcagggtct tcgccatata ggcgcgtgcc tgaccgagat tagcgtcaat aatcagcagc
    31981 gttttcatta tgcctctcct gctgtcagtt aaaaggttgt aagtcgacgc gcgccatcat
    32041 tgcggccaac tgcggacgat cggtaatacc cacattgctt tgacttaccg ccagggctgc
    32101 aacagctgtc gccagacgca gtgtgtgttc actggattca cgcatcagca agccataaat
    32161 caggccacca accatagaat cccctgcgcc aacggtgctt acgacatcga ctgacggtgg
    32221 tttggcgatc cattcgccgg aggcattaac ccaaagcgcg ccttcggcac ccagtgaaat
    32281 aacaacatgc gcaatacctt gttcacgtag cgcatgtgca gcttcaatca catctttcat
    32341 ttcaggcagt ttacggcctg cccagatttc caactcgcgg cggttaggtt tcaccagcca
    32401 cggtgccgct ttcaaacctg ctactaacgc ttcacggcta ctatcaaaga taatgcaagg
    32461 acactgacta cgcaggcgag tcatccagtc ggtgaacgct tccgggctga cgcctgacgg
    32521 taagcttccg ctgacacaga ccatatcgaa ctgaccgagc cagctcagag aatcagtcac
    32581 aaagcgttcc cagtcggcga gggtgacttc aaaacccgag aagttgaagt cggtcacttc
    32641 gccgtctttt tccgtcagct taacgttaat tcgggtgcgc ccctgtacaa cctggaaacg
    32701 gttggcaatg cccagctcgc tgaacagttg ctgaaaacca tcctgattgt ctttacccag
    32761 gaagccgcca acggtgacat caattcccag gtcttttaat actttggcca cgttgatgcc
    32821 tttacccgcc gcatgcagac cggtggtttt caccaggttc acttcgccgc gttcaatttc
    32881 cgggcagaaa ccaacaaggt cataagccgg attaagggtg atcgtagcaa cacgtctgct
    32941 cattatgcgc cctccccaag accagcagcg atagcgtcgc caatggcttt cagcgcctgt
    33001 tcagcatctg caccctgggc ggtaaagcgt aggcgatgac ctttcttaac gccaagtgcc
    33061 acaactttca tcagactacg tccgtttgcc ggtttgccgg tgccatcaag gtttgtcacg
    33121 gtaatatcac tgttaaattg tttaatggta ttgaccagca tggtacctgg acgagcatgc
    33181 aggccgtgtt cattgcgcac cacaaactcc gcgcttaaca cgtcgtcggt cggcgcatca
    33241 tcgctggtca gcagcgccag caacgttgcc gcatccgctt tcagcaagcg gtcagcttta
    33301 ttgtcgagca ataaatcagc gagacgctta agaaccgcga tgggctgatc gtcattcatc
    33361 gccacactca ccagcatggc tgccgtttcg ccgtccacat caaaagcatt tgccgcacgg
    33421 cttaccgcaa tcgcgctacg cagattgcct tcggcgctat cgctcagcca gataccctgt
    33481 ccgagattca gcggttgttc attgatggct ttggtgacga aagtggcgtc aactgccccc
    33541 acctctttca gacgcgcagc gttcagcgcc tgaagagtca gcagatcgct ggcgacgata
    33601 tccagcgtca gcatttcgtt gtcgagcttc agctgctcac tctgcttttc gcccatcagt
    33661 aatgcgcgaa gttcttctgc tgttgttgct gacttcagtt gttcagcaac ggaatcatcg
    33721 ctcagtacgt gggtcagctg gcgtagcagg cccagatgtt catccgagct ggcagcaata
    33781 ccgattgcca cgtacgctac ctgaccgtca ccccaggtga cgccttccgg gaactgaaat
    33841 acctgaacgc cggttttcag cacctgatcg cgggtgtcgg tagtgccgtg tggaatagca
    33901 ataccattgc cgaggaacgt tgaggtttgc tgttcgcgcg ccagcatgcc attgacgtag
    33961 ccttctgcta cattaccggc ctgcaccagc gccgcagcga cctggcgaat cgcctcttct
    34021 ttgtctccgg ccttttcgcc cggatggatg tcctgtacgg ataactggaa catagttctc
    34081 ctctcttgct gaattgaaac gattcagcct ctatgagaaa aaaagcgcca acctggctta
    34141 gggttaaaga caagatcgcg ctgaaacgtt tcaagaaagc ataatacttc tgtttcagca
    34201 cgcaaggaaa gccgtaaagt tatgagcaaa agtttgatgt gctgcacaat tttttgtcat
    34261 ttttcctaat tgctgatggg aaaatccgtt atcagcaatt tcatttcagc agcgtcagca
    34321 gccaatcagc aacagtgaaa tggcattttg attattcgtg gcgcttttga tttcgctgtc
    34381 tgtttatttt ctgacaagca gcgtaaactc cgcgtcttcc tcttccagtg atcgacctgc
    34441 atgcataact cccccgcagt ctccagcgcg aaatcgtttg acctgacctc gacggcgttt
    34501 ttaatcgttg cctttctcac cggtattgcg ggtgctctgc aaaccccgac actcagtatt
    34561 ttccttaccg atgaagtaca tgcccgtccg gcgatggtgg gattcttctt taccggcagc
    34621 gctgtcattg ggattctggt aagtcagttt ctcgctgggc gctctgataa gcgcggcgat
    34681 cgcaaatcgc tgattgtctt ttgctgcctg ttaggcgtgc tggcctgcac cctttttgcc
    34741 tggaatcgca actactttgt tttgctattc gttggcgtct ttcttagcag ctttggctcg
    34801 accgctaacc cgcaaatgtt tgcccttgcc cgtgaacatg ccgacaaaac cggacgtgag
    34861 gcggtgatgt tcagctcttt tttacgcgct caggtttcac tggcatgggt cattggccca
    34921 ccgctggctt atgcgttagc gatgggtttc agctttacgg taatgtatct gagcgcagcg
    34981 gtagcgttta ttgtttgcgg cgtgatggtg tggctgtttt taccgtcgat gcaaaaagag
    35041 cttccgctgg cgaccggcac ggttgaagcg ccgcgccgta accgtcgcga tacgctactg
    35101 ctgtttgtca tttgtacatt gatgtggggc tcgaacagcc tgtacatcat caacatgccg
    35161 ctatttatta tcaacgaact gcatcttccc gagaaactgg ccgatgtgat gatggggacc
    35221 gccgccgggc tggaaatccc gacgatgttg attgccggat atttcgccaa acgtctgggt
    35281 aagcgtttct taatgcgcgt tgctgccgtg ggtggcgtct gtttttacgc aggaatgctg
    35341 atggcgcatt cacctgtcat tctgttgggc ttgcagctgc taaatgctat ttttattggc
    35401 attctgggcg gcatcgggat gctctatttt caggatctga tgcccggtca ggcgggttca
    35461 gccaccacgc tctataccaa cacttcgcgc gtgggctgga tcatcgcagg atcagtggcg
    35521 ggcatcgtcg ccgaaatctg gaattatcac gctgtgttct ggtttgcgat ggtgatgatt
    35581 atcgccactc tgttttgctt actgcggatt aaagatgttt aaggcgcggt cagcatttcc
    35641 agatcgataa gccaggtcat ggcctgctga cgcgagttac cgcacatttc agcgctggct
    35701 tgtaagcctg cacagacttt cggtcgcagt ggcgaggtga aaattttaca gcgttgctgt
    35761 tcatcaagct gaatgcaggg cgtattggcg ggcttgccat ctggcatacc gggaataggg
    35821 ctggaaattg aaggggcagt gcaacacgcc ccgcaacccg gacggcattc catacgttgt
    35881 tctcttaagg ttaaccaccg gattgatgag cgcgcacagt accacctttt ttgcaccagc
    35941 aaaagtgcga ataccacttg cccgaaaggc ccgtcgcgag tactttgtcg cgatattttt
    36001 gacattttcg actacaggaa tttttcgatg ccaagagcga acgaaattaa aaaaggtatg
    36061 gtactgaatt acaacggcaa actgctgctg gttaaggata ttgatattca gtcgcccact
    36121 gcccgcggtg ccgctacgct gtacaaaatg cgtttttctg atgtccgtac cgggctgaaa
    36181 gtagaagagc gtttcaaagg tgatgatatc gttgacaccg tgacgctgac ccgccgttac
    36241 gttgacttct cctatgtcga tggcaacgaa tatgtcttta tggataaaga agactatacc
    36301 ccgtatacct tcaccaaaga tcagattgaa gaagagttgc tgtttatgcc agagggcggc
    36361 atgccggaca tgcaggtgct gacctgggac ggtcaactgc tggcgcttga gctgccgcag
    36421 accgttgatc tggaaatcgt tgaaaccgca ccaggcatca aaggggcatc tgccagcgcc
    36481 cgtaacaaac cggcgacatt gagcactggt ctggtgattc aggtaccaga atacttaagc
    36541 ccgggcgaaa aaattcgtat ccatatcgaa gaacgccgtt atatggggcg tgctgactaa
    36601 cttcagccgc atgcagaaaa gggatagctc aggctgtccc ttttttaatt tattatacca
    36661 atcttctatt ttgcgcttcc tgaacacctt acgccctcaa ttttcactcg ttgattgatc
    36721 gccctcacac ttcatcgcat taacaatcca gaccaatttc aattgctgtc atataacttt
    36781 acactgtcat tgttaattta tcgttactaa gacgtgactc ctatgaagac aattgcctcc
    36841 gttacgctcc cgcatcatgt acacgctcca cgctacgatc gccagcagtt gcaatcacgt
    36901 atcgttcatt ttggctttgg cgcctttcac cgcgctcatc aggcgttact gaccgatcgt
    36961 gtgctgaatg cccagggcgg cgactggggg atctgtgaaa tcagcttgtt cagcggtgat
    37021 caactgatga gccagctccg cgcacagaac catttatata ccgtgctgga gaaaggtgcg
    37081 gacggcaatc aggtgataat tgtcggtgcc gttcacgaat gccttaatgc aaaactggat
    37141 tccttagcgg caattattga gaaattttgc gagccacagg tggcaattgt ttccctgacg
    37201 attaccgaaa aaggctattg tattgacccg gccaccggtg cactcgacac cagtaatccg
    37261 cggattattc acgatctaca aacccctgaa gaacctcact ccgcaccggg tattctcgtc
    37321 gaagcactga aacgccgccg tgagcgcggc cttacaccgt ttaccgtgct ctcctgcgac
    37381 aatattcccg acaatggcca tgtggtgaaa aacgcggtgc tgggaatggc agaaaaatgt
    37441 tcgccagaac tcgccgggtg gataaaaaaa cacgtcagtt ttccgggaac catggtcgac
    37501 cgcattgttc cggctgcaac cgacgaatca ctggcagaaa tcagccagca tctgggggta
    37561 aatgatccct gcgcgattag ctgcgaaccg tttatccagt gggtggttga agataacttc
    37621 atcgctgggc gtcctgcctg ggaagtcgca ggtgtacaaa tggtgaatga tgtcctgcca
    37681 tgggaagaga tgaaactgcg tatgcttaat ggcagccact cttttctcgc ttatctgggt
    37741 tacctctcag gattcgtcca tatcagtgat tgcatgcagg atcgcgtatt tcgccatgct
    37801 gccagaacat taatgctgga tgagcaagcg ccgacactgc aaattaaaga tgtcgattta
    37861 acacaatatg cggataagtt aattgcacgt tttgctaatc cgtcgctgaa acataagacc
    37921 tggcaaatcg cgatggatgg cagccagaaa ttaccgcaac gtatgctggc aggtattcgc
    37981 atacatctgg ggcgtgaaac ggactggtcg ttgctggcat taggcgttgc tggctggatg
    38041 cgttacgtca gcggcgttga tgatgccgga aatgccattg atgttcgcga tccgcttagc
    38101 gataaaattc gcgaacttgt tgcgggcagc agcagtgaac aacgcgtaac cgccctgctt
    38161 tccctgcgtg aagttttcgg tgatgatctg ccagataacc cgcattttgt gcaggccatc
    38221 gaacaagcct ggcaacaaat cgtacaattc ggcgcacatc aggcgctatt aaacaccctc
    38281 aaaatttaac gatttctgcg gttaaagcgg atgaagctca ccttcgtccg ctctcccctt
    38341 ctcttttctg ccttttttag ccaggattaa cgctcagtta acttaccaga gtattgcgga
    38401 gccgttgtga ccaggaccaa cctcatcacc ggttttctcg gcagcgggaa aaccacgtcg
    38461 attcttcatc tgttagccca taaagatccc aacgaaaaat gggcggtact ggttaatgaa
    38521 tttggggaag tcggaattga tggtgctttg ctcgccgata gcggcgcatt gctgaaagag
    38581 atccccggcg gctgcatgtg ctgcgttaat ggtttaccca tgcaggtagg gttgaatacc
    38641 ttactgcgtc agggaaaacc agaccgcttg ttgatagagc cgaccgggct gggccatccg
    38701 aaacagatcc tcgatctgtt aaccgcacca gtctatgaac tgtggataga tctgcgcgcc
    38761 accttgtgca ttctcgatcc acgcctgctg ctggacgaaa aaagcgccag caatgaaaac
    38821 ttccgtgacc agctggctgc cgcagacatc attgtcgcca ataaatccga ccgtgcgacg
    38881 cccgaaagtg agcaagcgct acagcgttgg tggcagcaaa atggtggcga tcgacaatta
    38941 attcacagtg agcatgggaa agttgacggt catcttctgg atttgccgcg tcgcaattta
    39001 gccgagttgc ccgccagcgc cgcgcattct catcagcatg tcgtgaaaaa agggttagca
    39061 gcgttaagcc tgccagagca tcaacgctgg cgtcgcagtc tgaacagcgg gcaaggatat
    39121 caggcctgcg gctggatatt cgacgctgat acggtattcg acaccattgg cattctggaa
    39181 tgggcgcgac ttgcaccggt ggaacgcgtc aaaggcgtgc tgcgtattcc cgaagggctg
    39241 gtacgaatca accgtcaggg cgatgacctg cacattgaaa cgcaaaacgt tgcgccaccg
    39301 gacagccgta ttgagctgat ttccagcagc gaagctgact ggaatgcttt gcagagcgcg
    39361 ctgttgaagc ttcgtttagc gactaccgcg taaggttgcc tgcgtttttc agtaagataa
    39421 ttagagaaaa tatgattaaa aatttgccgc aaatagtgtt gttgaatatt gtcggcctcg
    39481 cgctgtttct ttcctggtat attcccgtta atcatggatt ctggttgccg attgatgcgg
    39541 atatttttta tttctttaat cagaaactgg tcgaaagtaa ggcctttttg tggctggttg
    39601 cattgaccaa caatcgcgct ttcgacggtt gttctctgct ggcgatggga atgttgatgc
    39661 tgagtttctg gctgaaagaa aacgcccctg gcagacgacg tatcgtgatt atgggtctgg
    39721 tcatgctatt aactgcagtg gtattaaacc agctgggtca ggcattaatt cctgtaaaac
    39781 gggccagccc aacattgact tttaccgata ttaaccgcgt cagcgaactg ctctctgttc
    39841 ccacgaaaga tgcctcacga gatagctttc ccggcgatca cggcatgatg ctgcttattt
    39901 tttcggcatt catgtggcgt tatttcggca aagttgcagg ccttatcgcc cttattattt
    39961 ttgtggtttt tgcatttccc agagtaatga ttggcgcaca ctggtttact gacatcattg
    40021 tcggttcgat gaccgtgata ttgatcggtt tgccctgggt gttgctgacg ccattaagtg
    40081 atcgattaat cacctttttt gacaaatcac taccgggaaa aaacaaacat ttccaaaaca
    40141 aataactcac agtaattaac atcatcaggc ttatttttat agtgaggata atcctgatga
    40201 tgcgcaccgt gctttcatcc atcgcgcgca aaaatcattc tctaagtaaa tgaatggatt
    40261 gcatgcgttt cactcaatcg tactttaatt gaccaatccc gcttattaac tttctgtatc
    40321 actttttctt ataaaaaatc atgtaaaacc gctcgccaag accgcaccaa tcgggtaatc
    40381 tagaactcgt tttgcctcgg cggtagatta tcctcacagc atataatttt gtgcgttagt
    40441 ccacagattt ggccttaagg aattgtttca acatgcccag gtaattagtc tcgtgtcgct
    40501 tggcattttt ttataacgat atttgtcgtt aaggacttca agggaaaaca aacaacatgg
    40561 tcaaatctca accgattttg agatatatct tgcgcgggat ccccgcgatt gcagtagcgg
    40621 ttctgctttc tgcatgtagt gcaaataaca ccgcaaagaa tatgcatcct gagacacgtg
    40681 cagtgggtag tgaaacatca tcactgcaag cttctcagga tgaatttgaa aacctggttc
    40741 gtaatgtcga cgtaaaatcg cgaattatgg atcagtatgc tgactggaaa ggcgtacgtt
    40801 atcgtctggg cggcagcact aaaaaaggta tcgattgttc tggttttgta caacgtacat
    40861 tccgtgagca atttggctta gaacttccgc gttcgactta cgaacagcag gaaatgggta
    40921 aatctgtttc ccgcagtaat ttgcgtacgg gtgatttagt tctgttccgt gccggttcaa
    40981 cgggacgcca tgtcggtatt tatatcggca acaatcagtt tgtccatgct tccaccagca
    41041 gtggtgttat tatttccagc atgaatgaac cgtactggaa gaagcgttac aacgaagcac
    41101 gccgggttct cagccgcagc taataaaccg tttggatgca atcccttggc tatcctgacg
    41161 agttaactga aagcactgct taggcagtgc ttttttgttt tcattcatca gagaaaatga
    41221 tgtttccgcg tcttgatcca ggctatagtc cggtcattgt tatcttttaa atgttgtcgt
    41281 aatttcagga aattaacgga atcatgttca tacgcgctcc caattctgga cgtaagctcc
    41341 tgcttacctg cattgtagca ggcgtgatga ttgcgatact ggttagctgc cttcagtttt
    41401 tagtggcctg gcataagcac gaagtcaaat acgacacact gattatcgac gtacaaaagt
    41461 atctcgatac ctattttgcc gacctgaaat ccactactga ccggcttcag ccgctgacct
    41521 tagatacctg ccagcaggct aaccccgaac tgaccgctcg cgcggcgttt agcatgaatg
    41581 tccgtacgtt tgtgctggtg aaagataaaa aaacattctg ttcatctgcg actggtgaga
    41641 tggacattcc actcaatgaa ttgattccgg cgctcaacat taataaaaac gtcgatatgg
    41701 cgatcttacc cggcacgccg atggtgccga acaaacccgc aatcgtcatc tggtatcgca
    41761 accctttgct gaaaaatagc ggtgtgtttg ccgctctgaa tctcaacctg acgccttctc
    41821 tcttttatag ttcacggcag gaagattacg atggcgtcgc cctcattatt ggcaatactg
    41881 cgctatctac cttttcttca cgtttgatga acgttaatga attaaccgac atgccggtcc
    41941 gtgaaactaa aattgcgggc attcctctga ccgttcggct ttatgcggat gactggacat
    42001 ggaacgatgt gtggtacgca tttttactgg gcggcatgag tggaactgtc gttggcctgc
    42061 tctgctatta cctgatgagc gtacgtatgc gccccggcag agaaatcatg accgccatca
    42121 agcgcgaaca attttacgtg gcgtatcaac cggtggtgga tacacaagct ttgcgagtaa
    42181 cgggcctgga agtactgcta cgctggcggc atcctgtcgc gggagaaatt cccccggatg
    42241 ccttcattaa ctttgccgaa tcgcaaaaga tgattgtgcc gctgactcag cacctgtttg
    42301 agttaattgc ccgcgatgcc gcagaattag aaaaagtgct gccagtaggc gtcaaatttg
    42361 gtattaacat tgcgccggac catctgcaca gcgaaagctt taaagcagat atccagaaac
    42421 tgctcacttc cctgcccgca caccatttcc agattgtgct ggaaattacc gagcgcgata
    42481 tgctgaaaga gcaagaagcc acacaactct tcgcctggct gcactcggtc ggcgtagaaa
    42541 ttgctattga tgacttcggc accgggcaca gcgcgcttat ctatcttgag cgttttacgc
    42601 tcgattatct gaaaattgac cgtggattta tcaacgccat cggtacggaa acgatcactt
    42661 cacccgtact tgacgcggtg ctgacgctgg cgaaacgcct caatatgctg acggttgctg
    42721 aaggggtcga aacgccggaa caggcgcgat ggctaagcga acgcggcgtt aatttcatgc
    42781 aaggctactg gattagtcgc cctttaccgc tggacgattt tgtccgctgg ctgaagaaac
    42841 cgtatacgcc gcagtggtaa ggtgtgctta cgtcccttat tattcatagt gaaagcatgc
    42901 cggattgcgg ctaatgatga gtaaaaggaa atccattgca gatgattgtg cgcatactgc
    42961 tgctgtttat cgctctgttc acctttggtg tgcaggcgca ggctatcaag gaaagctatg
    43021 cctttgccgt gctgggcgaa ccccggtacg cgtttaattt caaccatttt gattatgtga
    43081 accccgccgc gccaaaaggt gggcagataa cgttgtcagc cctcggcacc ttcgataatt
    43141 tcaaccgcta tgcactgcgc ggcaacccgg gcgcacgcac cgagcagctg tacgacacgc
    43201 tatttacgac ttccgatgac gaaccaggca gttattaccc gctgattgct gaaagcgcac
    43261 gctatgctga cgattattcc tgggtggagg tcgctattaa tccgcgcgcc cgttttcatg
    43321 atggttcgcc cattactgcc cgcgatgtag agtttacttt tcaaaaattt atgaccgaag
    43381 gcgtgccgca atttcgtctg gtctacaaag gcaccaccgt caaagccatt gcaccgttaa
    43441 ccgtgcgcat tgagttagct aaacccagca aagaagatat gctgagtctg ttttcgctgc
    43501 cgatatttcc agaaaagtac tggaaggatc acaaacttag cgacccgctc gccacgcctc
    43561 cgcttgccag tggtccgtac cgcgttacgt cctggaaaat ggggcaaaat attgtctatt
    43621 cccgcgtgaa agattactgg gcagcaaact taccggtaaa ccgtggacgc tggaatttcg
    43681 acaccattcg ctacgattat tacctcgatg ataatgtcgc ctttgaagcg tttaaagcag
    43741 gtgcctttga tttgcgtatg gaaaacgacg ctaaaaactg ggccacgcgt tataccggta
    43801 aaaatttcga taaaaaatac atcatcaaag atgagcaaaa gaacgaatca gcccaggata
    43861 cgcgctggct ggcgtttaat atccaacgtc cggtattcag cgatcgccgg gtccgggaag
    43921 caatcactct cgcctttgac tttgaatgga tgaacaaggc gttgttttac aacgcctgga
    43981 gtcgcacgaa cagttatttt cagaataccg aatacgcggc cagaaattac cccgacgccg
    44041 cggagctggt gcttctggca ccaatgaaaa aagatctacc gccagaagtc ttcacacaaa
    44101 tctaccagcc gccggtatct aaaggcgatg gctacgatcg tgacaacctg ttaaaagccg
    44161 acaaacttct caacgaaacg ggctgggtgc tgaagggtca gcaacgcgtt aatgccacaa
    44221 cgggtcagcc gctcagcttt gaattattgc ttcccgcaag cagcaatagt cagtgggtat
    44281 tgccgttcca gcacagcctg caacggctgg gtatcaatat ggacattcgc aaggtggata
    44341 actcgcaaat cactaaccgc atgcgcagtc gcgactatga catgatgccg cgcctatggc
    44401 gggcgatgcc gtggcccagt tccgatttac agatttcctg gtcatcggaa tatatcaatt
    44461 ccacttataa tgcccccggc gtgcaaagcc cggttatcga ctcgctgatc aaccaaatta
    44521 ttgccgcgca gggaaataaa gaaaaattac tgccgttggg gcgagcactg gatcgcgtat
    44581 taacgtggaa ttattacatg ctgccaatgt ggtacatggc ggaagaccgt ctcgcctggt
    44641 gggataaatt ctcccaaccc gctgtacgcc ctgtttacag cctgggtatc gatacctggt
    44701 ggtatgacgt taataaagcg gccaaactgc cgtcagccag acaacaggga gagtagatgg
    44761 gcgcttacct gattcgccgt ctgttgctgg tgatcccaac gttatgggct attatcacta
    44821 tcaacttttt catcgtgcaa attgcgcctg gcggtccggt cgatcaggcc atcgccgcca
    44881 ttgagtttgg taatgccgga gtattacccg gcgcaggcgg tgaaggtgtt cgtgccagcc
    44941 atgcgcaaac gggtgtcggc aatatcagcg acagtaatta ccgtggcgga cgcggattag
    45001 acccagaagt gatcgctgag atcactcatc gctacggttt cgataagccg atccacgaac
    45061 gttacttcaa aatgctctgg gactacatcc gctttgattt tggtgatagc ctgtttcgca
    45121 gcgcctcggt gctgacgctg attaaagaca gtctgccggt ttccatcacc ctcggattgt
    45181 ggagcacgct gattatctat ctggtgtcga ttccgttagg cattcgcaaa gctgtttata
    45241 atgggagtcg ctttgacgtc tggagtagcg catttatcat catcggctac gccattccgg
    45301 cctttttgtt tgccatcctg ctgattgtct tcttcgcggg cggcagctat ttcgacctgt
    45361 tccctctacg cggcctggtt tccgctaact ttgattcgct gccgtggtat cagaaaatca
    45421 ccgattatct gtggcatatc acgctaccgg tgctggcgac agtgattggt ggctttgcgg
    45481 cgctgaccat gctgaccaaa aactcattcc ttgatgaagt gcgcaagcaa tacgtggtga
    45541 ccgcgcgtgc gaaaggggta agtgagaaaa acattctctg gaaacatgtg ttccgcaacg
    45601 ccatgctgct ggtgattgcc ggttttccgg cgacgtttat cagcatgttt tttaccggct
    45661 cgctgctgat tgaggtgatg ttttcactca atggtcttgg cttactgggc tacgaagcga
    45721 ccgtctcgcg cgattatcct gtaatgtttg gtaccttgta tattttcacc ctgattggcc
    45781 tgctgctgaa tattgtcagt gatatcagct atacgctggt tgatccgcgt atagattttg
    45841 agggacgtta atgtcgcgac tcagccccgt caatcaggcc cgttgggcgc gttttcgtca
    45901 taaccgtcgc ggctactggt cgttatggat tttcctcgtt ttgtttggtt tgagtttgtg
    45961 ttctgaactt atcgctaacg ataaaccgtt gctggtgcgt tatgacggca gttggtattt
    46021 cccgttgttg aaaaactaca gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga
    46081 ttatcaggac ccgtggctga aacaacggct ggaaaataac ggctgggtac tgtgggcacc
    46141 gattcgcttt ggtgctacca gtatcaactt tgctaccgat aagcccttcc cttctccacc
    46201 ctcccggcaa aactggctgg gaacggatgc caacggcggc gatgtgctgg cacgcattct
    46261 ctatggcacg cggatctcgg ttctgtttgg cctgatgctg actctctgtt ccagcgtgat
    46321 gggcgtgctg gcgggggcgc tacaaggcta ttacggcggt aaagtcgatc tctggggaca
    46381 acgctttatt gaagtatggt cggggatgcc gacgctgttt ttgattattt tactttccag
    46441 cgtcgtacag cctaacttct ggtggctgct ggcaattact gtcttgtttg gctggatgag
    46501 tctggtcggc gtggtgcggg cggagttttt acgtactcgt aatttcgact acattcgtgc
    46561 ggcacaggcg cttggcgtca gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc
    46621 catggtcgcg accctcacct ttttaccgtt tattttatgt agttcgataa ccaccctgac
    46681 ctcgctcgat ttcctcggct tcggtctacc gctcggttca ccgtcactcg gtgaactgct
    46741 gttacaaggg aaaaataacc ttcaggcccc gtggcttggg atcaccgcct tcttgtcggt
    46801 ggcgatatta ttgtctttgc tgatctttat tggtgaagcc gtccgcgacg catttgatcc
    46861 taataaggcg gtgtagcatg acgcaaactc tgttagcgat tgaaaatttg tcggtgggtt
    46921 ttcgccatca gcaaaccgta cgtacagtag tcaatgatgt ttcactacag attgaggctg
    46981 gcgaaacgct ggcgctggtg ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa
    47041 ttttacgcct gctcccttcc ccgccggttg aatatctctc cggcgatatt cgttttcatg
    47101 gcgaatcgct gcttcatgcc agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg
    47161 ccatgatttt tcaggaaccg atggtgtcgt taaatccatt gcataccctg gaaaaacagc
    47221 tttatgaagt gctttcactc caccgcggga tgcgtcggga agcggctcgt ggcgaaattc
    47281 ttaactgcct tgatcgcgtc ggtatccgcc aggcggcaaa acggctgaca gattatccgc
    47341 atcagctctc cggcggcgaa cgccagcggg tgatgattgc gatggcgctg ttaacgcgac
    47401 cggaattatt aattgccgat gaaccgacca ccgcactgga cgtctctgtc caggcgcaga
    47461 ttttacagct gttgcgcgaa ctgcaaggcg agctgaatat gggcatgctg tttattactc
    47521 ataacctcag cattgtcaga aaactggccc accgcgtggc ggtaatgcaa aacggtcgct
    47581 gtgtcgagca aaataacgcc gctacgctat ttgcctcacc cactcatcct tacacacaaa
    47641 agctactcaa cagtgaaccg tcaggcgatc cggtaccgtt gccagaacct gcctcaacgt
    47701 tgctggatgt tgaacagctt caggttgcct tccccattcg caaagggatt ttgaagcgca
    47761 ttgtggatca taatgtggtg gtgaaaaaca tcagttttac gctacgggcg ggtgaaacac
    47821 tgggtttagt gggcgagtct ggttccggga aaagcacgac gggactggcg ctgctgcgac
    47881 tgattacctc tcagggcagc atcgtctttg acggtcagcc actgcaagat ttaaatcgcc
    47941 gccagctgtt acctactcgt catcgtattc aggtggtatt tcaggatcca aactcctcgc
    48001 tcaacccacg actcaacgtt ttgcagatta ttgaggaagg cttacgggtt caccagccga
    48061 cgctttctgc cgcacaacgc gaacaacaag tgatagccgt gatgcatgaa gtgggattag
    48121 atcctgaaac acgccaccgt tatccggcgg agttctctgg tggtcagcga caacgtattg
    48181 cgattgccag ggcattaatt cttaagccct cactgatcat acttgatgaa ccgacatcat
    48241 cactcgacaa aacggtacag gcgcaaatat tgacgctatt gaaatcattg caacaaaagc
    48301 atcaactggc ctatttgttt atcagccacg atttgcacgt tgtccgcgcg ttatgtcatc
    48361 aggttatcgt actgcgacaa ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg
    48421 ccgcaccgca gcaggagtat acgcgtcagc tactggcgtt gagctgacgc ttaaaaagga
    48481 ttgtagtttg aaaagggttc ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca
    48541 gcaaactcgt cctggcgatt tacaaacagg cacggctcac cttcacactc cagcaccgaa
    48601 catggaactt cgatgtcgct taacgcctgg cttagtttat acatcaccga ccatgcatta
    48661 tcaccatccg ggctaagcaa tttaagcgcc accaggctgt tgtcaccgca cggtccattt
    48721 tgcggaatcg gttcaacctt cgaacctgcg aaaccagcag accagcgaaa gttctgcggt
    48781 agacgatgga cgattgagag ttgtaatgaa gtcacttatt ttccccggaa gcacatttac
    48841 ttcacaattt atttacatca attttaacac atcatcaaca atccgtcttt atacagatcg
    48901 taaagattga tgcctcgcct gcatggcctc tatggctctg atttaagtaa tatctgcggg
    48961 cgtggtcacg tttgcggggc tatatgtacc cgtttctgcg atctaactca acctttttaa
    49021 ctacaatgat gtgacttttt acataaattg attttacata aaataaacat atatcggggg
    49081 aatgatagat ttgtggttga catgcatcaa caaaggaagc cttttagctt cctcgttgtg
    49141 caatagatca ccgttttttc ggtcgactgg cgtacagaca gaagagaatg gagctggttg
    49201 cgcagaatgc aattgaccaa atcatcggcc atgcagagtt aaaggtcgca agagaaagca
    49261 atgcgccaac aattgcccct atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc
    49321 ccgccatatg gggaaactca tcaagaatga ccgccatcgc attggatgac accatagaca
    49381 cgcagcccac aaacgccgca acgccaacca ccagcgacca gaatcccagc cccagcagcg
    49441 cactgatgac catccacgct gccataataa attgtatcca caaccccgag cggaacatat
    49501 ttaacgcgcc aatgcggcgg acgaagcggc tgttaaagat ggtcatcacg aacagaaaaa
    49561 caatgtttag cgcaaagtaa taaccaaagt tttccggtgc aacgtggttg atttcaatgt
    49621 agacaaacgg tccggcgctc aggaacgaga acattccggc aaagctgaaa ccgctggcca
    49681 gcatataact caggacacgc ttatgacgga acagcgccgc aaagttacca atagtggtac
    49741 gaatgtgaaa aggctggcga ggatccagaa gatgtaatgc cagctcagcc acaccagcac
    49801 ccagccgcca actatcggtg ccatcagcgg tgcaatggtc gtcaccagca tgacaaacga
    49861 catcatccgc gagaactctt ctttcgggta aatatcacgc atcaaagcgt tgataaccac
    49921 gctggccgca gccgcagcca gcccgtggaa gaaacgcatc acaatcagct gatcgatggt
    49981 ttgtgccaac gcacacgcca ccgcggcggc ggcaaacacc agcgtaccgc caagaaccac
    50041 cggcttacgc ccgaagctgt ctgccatcgg cccgtagatt aactgcccca acgcaaagcc
    50101 cagaatataa gtactcaggg tcatctgcgt actgcccgcc ggtacgccaa actgcgctga
    50161 aattaccggt agcgcgggca gatacatatc aatcgacagc ggcatcaaca tggccagcag
    50221 gccaaggata aaaacaatag caaacgacga atgctgtcgg gtggtcacaa cgggctcctg
    50281 aaagtcattg aaaagttaga cgacgctggc aatttcttct tcagttaacg gacgatattc
    50341 accgggggct aaatcagcat ccagcgtaat gccgccaata cgttcacgat gcagctcaac
    50401 cacatggtta cccacggcgg cgaacatgcg tttcacctga tgataacgcc cttcgctgat
    50461 ggtcagacga acctgcgttg gggtaatcac ttccagcacc gccggcttag tgagatcttt
    50521 ttcgttatgc agctgcacgc ctttagcaaa ttgttctgcc gtatcgtcag ctacgggtga
    50581 taccagcgtc accagatagg tcttctcgca atgatggcgc ggagaagtaa tgcggtgcga
    50641 ccactgacca tcatctgtca ttagtaccag accggtggta tcgatatcca gccgccctgc
    50701 ggcgtgcagt ttccacgcta ccggttcatc tagaaaatag agcaccgttg ggtgatcagg
    50761 gtcgtccgtg gagcaaacat aaccctgagg cttattgagc atgaagtaac gtggaccgtg
    50821 ttgctgcgcc agcgggttgc catcgtaagc gacatcatgt tcaggaagca gtttgaacgc
    50881 tgcattacgg acgatttcgc catcgacggt gacacgattg ccgcggattt cacgcccggc
    50941 aatagcacgg ctaacgccga gttgctgtgc gataaattta tcaagtcgca tgtgtgtgat
    51001 tttgcctgta aaaaacggag gtcgggtaat gacccgaata tctgaactgt tgtctgccca
    51061 gtatagcggt ctaataacat ccctcaaggg aaaaagatcc gtggcatact attagcagaa
    51121 taatctacct acgcgagacc atgattttta cacttcgccc atatcagcaa gaagccgtgg
    51181 atgccacgct caaccatttt cgtcgtcata aaacccctgc cgttatcgtg ctgcccaccg
    51241 gtgcaggtaa aagcctggtg atagcggaac tggcacggct ggctcgtggt cgcgtactgg
    51301 tgctggcaca cgttaaagaa ctggtggcgc aaaaccatgc aaagtatcag gcgctggggc
    51361 tggaagccga tatttttgcc gccgggctga agcgtaaaga gagccacggt aaagtggtat
    51421 ttggcagtgt gcagtcggtc gcccgtaata ttgatgcctt tcagggtgaa ttttcgctgt
    51481 tgattgtcga tgaatgtcac cgtattggtg acgatgaaga gagccagtat cagcaaatcc
    51541 tcactcacct gacaaaagtg aatccccact tacgcctgct ggggctgact gccacgcctt
    51601 ttcgattggg caaaggttgg atctaccagt tccattatca cggcatggta cgcagcgatg
    51661 agaaagccct tttccgtgac tgcatttatg agctgcccct gcgttatatg attaaacaca
    51721 gctatctgac gccgccagaa cgactggata tgccagtagt gcaatatgat ttcagccgct
    51781 tgcaggcaca gagcaacggg ctgttcagcg aagccgatct caaccgtgag ctgaaaaaac
    51841 agcaacgtat taccccgcac atcatcagcc agattatgga gttcgccgaa aagcgtaaag
    51901 gagtgatgat tttcgccgcg acggtcgaac acgcaaaaga gattgtggga ttactgcctg
    51961 ccgaagatgc agcactgatt actggcgaca cccccggcgc tgagcgcgat gtgttaattg
    52021 aaaattttaa agcccagcgt tttcgctatc tggtcaacgt cgcggtactg accaccggat
    52081 ttgacgcccc gcacgtcgat cttatcgcca ttctgcgccc taccgaatca gtgagtcttt
    52141 accaacaaat tgtcgggcgc ggtctgcgtc tcgctccggg caagactgat tgtttaattc
    52201 ttgattatgc gggtaatcct cacgatctct acgcgccgga agttggtaca ccgaaaggca
    52261 aaagtgacaa cgttccggta caggttttct gccctgcctg cggttttgcc aacacctttt
    52321 gggggaaaac gaccgccgac gggacattga ttgaacactt tggtcgccgc tgtcagggat
    52381 ggtttgaaga tgacgacggt catcgcgaac agtgtgactt ccgtttccgt tttaaaaatt
    52441 gcccgcaatg taacgcagaa aatgatattg ccgctcgccg ctgccgcgaa tgtgacacgg
    52501 tactggttga tccggatgat atgttaaaag cggcgctacg actgaaagat gcgctggtat
    52561 tacgctgtag cggcatgtct ttgcagcatg ggcacgacga gaaaggcgaa tggttgaaaa
    52621 tcacctatta cgatgaagac ggtgcggatg tgagtgagcg tttccgtctg caaacacctg
    52681 cccagcgtac cgccttcgag cagcttttta tccgcccgca tacgcgcaca ccgggcatcc
    52741 cgctgcgctg gatcaccgcc gccgatatcc tcgcccagca agccttattg cgacccccgg
    52801 attttgtcgt cgcccgcatg aaaggccagt actggcaggt gcgtgaaaaa gtgttcgatt
    52861 acgaaggtcg ttttcgtcgg gcgcacgaat tacgcggtta atggtagttt tgattgatgt
    52921 ataaggcgat tgagtataga atctcacccg cttttgcata cgcaaagcag atcacttacc
    52981 tgttgctggg tcgcctgtag caggattaat ttaagagaga aagaaatgtt tactatcaac
    53041 gcagaagtac gtaaagagca gggtaagggt gcgagccgcc gcctgcgtgc cgctaacaag
    53101 ttcccggcaa tcatctacgg tggcaaagaa gcgccgctgg ctatcgagct ggatcacgac
    53161 aaagtcatga acatgcaagc taaagctgaa ttctacagcg aagttctgac catcgttgtt
    53221 gacggtaaag aaatcaaagt taaagctcag gacgtacagc gtcacccgta caaaccgaag
    53281 ctgcagcaca tcgacttcgt tcgcgcttaa ttgctgaata agttgtaaaa aaccccgctc
    53341 ccgcggggtt ttttgtatct gcagattatg cctgattacg gtattgctat tttttgcagg
    53401 ccagataagg cgttcacgcc gcatctggca ttcagaacac aaaaccgatt aattgccgcc
    53461 agacgtccgg cgctgcaatt ggtcgcgcaa attcggcggc gtgcctttaa tggtcagggt
    53521 atcggtcgcc ggctcccaga agatgcgttc acctaacagc atcgcatcaa agttaatagt
    53581 caagccaccg ccgctaccgg caaacttcgt caactgacgc agtgtgctcc ggtctgccgg
    53641 gaagctctct tccagttcgt agcctttttc agccgcaaac tccgtgaagc tcacttcgct
    53701 tacgcctgcc agctctttcg acagcgattc cagttcgatc tcttcgcctg cctgcagctg
    53761 ctcattacag tagctataaa cctgctggcg cacgttctgt cgctctgctt tatccagttg
    53821 tgcttcggca gtgaaatcat caaccgcttg caacagccca cggttttgtg ctttcgtgtt
    53881 caatccttcg ctggccccga ggaaatccat aaagaaatcc gccactttgc gccctactcg
    53941 ccctttcagg aaagtgagat agcgggtgga ctctggattg gtttcccatt cggttaaatc
    54001 gatacgcgca acaatatccg catgattgat atcaagataa tgggttgggt tgatgtcgag
    54061 attttcgtta acacgcatac tgctcaggtt gctcagaact gccaccagca gatactccac
    54121 cgccagataa cggtagtggc aaaacagcac gaatccaccg tcagcaaaag gatatttcgc
    54181 taattcgtca cgcaggcgac cagttgccgc gcggctaaat gccagaaagt cctcttcgcc
    54241 ctgacgctgt aagcgcagcg tctgcgccag ttcactctct tcgctaaaca agccgtaagc
    54301 tttatttttg gcgctataga cccgatgcag ttctgccacc atctcaacga cggtttctgt
    54361 cggttccagc aatgaatcgc gcaacaccag ctcaaggttc tgctcatcac gcttgataag
    54421 ctggtgcaga gcaatctggt tgatatccag actcatgata aactctcctt taagaccggg
    54481 cggtattcaa ccaccgccgg ttaagcgacg caataaaaga tggcggtcat tttacagaag
    54541 gcagcgtaac gagaatgatt taacgcataa aaaagcagaa aaaaaaccgt tgctacggta
    54601 atatgttgcc ctttcatcaa ctaactgatt tcgatttatg ccacaaattt cccgctacag
    54661 tgatgaacag gttgaacaat tgctcgcaga gctgctcaac gtactggaaa aacataaggc
    54721 tccgaccgac ctttccctga tggtgttagg caacatggtc actaacctta tcaacaccag
    54781 cattgccccg gcccaacgcc aggcaattgc caactctttt gcccgcgcct tacagtcctc
    54841 tatcaacgaa gacaaagcgc actaagggaa acagataaca ggttatggta actcatcgtc
    54901 agcgctaccg tgaaaaagtc tcccagatgg tcagttgggg gcactggttt gcactgttca
    54961 atattctgct ttcgctcgtc attggcagcc gttacctgtt tatcgccgac tggccgacaa
    55021 cgcttgctgg tcgcatttat tcctacgtaa gcattatcgg ccatttcagc ttcctggtgt
    55081 tcgccaccta cttgctgatc ctcttcccgc tgacctttat cgtcggctcc cagaggctga
    55141 tgaggttttt gtccgtcatt ctggcaacgg cgggaatgac gctattactg atcgatagcg
    55201 aagtctttac tcgtttccat ctccatctta atcccatcgt ctggcagttg gttatcaacc
    55261 cagacgaaaa tgagatggcg cgcgactggc agctgatgtt catcagcgtg ccggttattt
    55321 tattgcttga actggtgttt gcgacgtgga gctggcaaaa gctgcgcagc ctgacgcgtc
    55381 gtcgacgctt cgcgcgcccg ctggccgcat tcttatttat cgcctttatc gcctcgcatg
    55441 tggtgtatat ctgggccgat gccaacttct atcgcccgat caccatgcag cgcgctaacc
    55501 tgccgctttc gtacccgatg acggcgcgac gttttcttga gaagcatggt ctgcttgatg
    55561 cgcaggagta tcaacgccgt cttattgagc aaggtaatcc agacgccgtt tccgttcagt
    55621 atccgttaag cgaactgcgc tatcgcgata tgggcaccgg gcagaatgtg ctgttgatta
    55681 ctgtcgatgg cctgaactac tcacgcttcg agaagcagat gcctgcgctg gcaggttttg
    55741 ctgagcaaaa tatttcgttc acgcgccata tgagctccgg caacactaca gacaacggca
    55801 tctttggcct gttctatggt atctcgccga gctatatgga cggcattctg tcgacccgta
    55861 cgcctgcggc attaattact gcgcttaatc agcaaggcta tcagctgggg ttattctcat
    55921 cagatggctt taccagcccg ctgtatcgcc aggcattgtt gtcagatttc tcgatgccga
    55981 gcgtacgcac ccaatccgac gagcagaccg ccacgcagtg gatcaactgg ctgggccact
    56041 acgcacaaga agataaccgc tggttctcgt gggtttcttt caatggcact aacattgacg
    56101 acagcaatca gcaggcattt gcacggaaat atagccgggc ggcaggcaat gtcgatgacc
    56161 agatcaaccg cgtgctcaat gcactgcgtg attctggcaa actggacaat acggttgtga
    56221 ttatcactgc cggtcggggt attccgctga gcgaagacga agaaaccttt gactggtccc
    56281 acggtcatct gcaggtgcca ttagtgattc actggccagg cacgccggcg cagcgtatta
    56341 atgcgctgac tgatcatacc gatctgatga cgacgctgat gcaacgcctg ctacatgtca
    56401 gcacacctgc cagcgaatat tcgcaaggtc aggatttgtt caaccctcaa cgccgtcatt
    56461 actgggttac tgcagcggat aacgatacgc tggcaattac caccccgaaa aagacgctgg
    56521 tgctgaacaa taacggtaaa taccgcactt acaacttacg tggtgaaaga gtgaaagatg
    56581 aaaaaccaca gttaagtttg ttattgcaag tgctgacaga cgagaagcgt tttatcgcta
    56641 actgattaat tataaatcag ttagcgaaat atcttacttg caatcggtgt ggaaaacggt
    56701 agtattagca gccacgagtc ggcacgtagc gcagcctggt agcgcaccgt catggggtgt
    56761 cgggggtcgg aggttcaaat cctctcgtgc cgaccaaaaa tcccaagaaa aaaccaacct
    56821 ttacggttgg tttttttata tctgcaatta atttgataaa cagaccgtga cacatcacag
    56881 cctgtttatt ttctgttatc agaacgtcca gaccactccc gcctgagtat tccacggcga
    56941 ttctacccct gcgccattgc catagctgac tgacaaatga ccgcttaacg tcggggtaaa
    57001 cgatgacctt atcccagcct gataaacgcc acgggttcca gccacatcat tattaaattt
    57061 gccatcgtca ttaactttca catggttaga atcggcgtat tcctgacgca cggcggcttt
    57121 cagccagggt tccaacgtcg taccgttttg caggtccatg tgatagctta ccgccgttcc
    57181 cgcttcagcg cgtaatatcc gggtatttcc cacatccgcg cgcatgccgt ttgataacgt
    57241 gtagtcctga ccatctgtgg taaagccggt aaaggcgaga tagggtctaa cactccacaa
    57301 tccgtcaacc caacggaacc cgctctcaac atgagcaccc gcgccgttac tattgtaatc
    57361 gccaaacgct gttgccccat tactcatctt gccatggatg gtgttggcaa aacggtcaac
    57421 tttcaccacc ccatcaacat aggcaccgtt ctgatgctcc caaccggcat aagcccccag
    57481 ggtgtagcta tcaatattgc ctttgccgcc gcgatcaaaa ccaatatcag aatgagagta
    57541 atcaaagaac aagccgcgaa ttgtgctgct ttcttcacgg gagaaacggc tatcgatacc
    57601 gagcgtcagg cccgtcaatg tttgctcaaa gccagctccc gcatcagtgg tcacgttgtt
    57661 gcgggtgtta attgccgaac tccacatcgc cgtatcgtaa ttaacgcctt ttacgctacc
    57721 aagacgctca cgcacggtgt ccagttctgc atcaaatacc agcggttgtg ctgctgccat
    57781 gttcagcaca tcagtggttg aaggggtaat ttgcgcgcga ttctctgcga ggctccagct
    57841 atggttgcca ttatccagca aggtatattc atacgtaccg atatcaacaa cgcctccggc
    57901 attgcccaac gtgaatgcag catcaccgcc gcccgtcgtt accagtgtaa ggctatctcc
    57961 tgctgccggg ctggcccccg tgtccgtcac gaatattttg aaatcacctg ttgcctgacc
    58021 gatgacgttg agctgatcgc tctgatgatt agccatatcg gtacgcatat aaaattgccg
    58081 tttcccgaca gggtattggt ggtcaacgta atgaaatttt ccgcacttgc cgtcacagat
    58141 gaacgagtca ctggctcagc catcataatc acatttgcgc cggacatcgc aaggttggct
    58201 aagttgaggc gagtgttttg gttcgtcgta gtgatatcgg caatgatggt ccatgcgcta
    58261 ttttcgagcg aaacgtccgc ttggctggta tccacggcac catgcgttct aaaagaagcg
    58321 ccctcagaaa ccactacctt acccaccaac atagcgggtg cataatttat tttcggcttc
    58381 atgacctcaa gaatgccatc attccctctg actgacacgt taaccattgt gccagcccag
    58441 acattagcgc ggccattgtt gataaccata ttttcagaga tggctttaga ggaatacgtg
    58501 atacttccat tctgatctga tcgaccgaga tcatatacag catttgcctg cactttcgta
    58561 ccagtatcct ttcccagcga ttgcatagtg gcatgctcat caaggatagt gtcaatggcc
    58621 tgagtgtcct ccataacaat gagcccggag ccatcatcca gttcataatt ttttgacaca
    58681 ccatccttta tactaaattg gcctttactg tttgtaccac tcacttccag cgcattcgtt
    58741 gagacaatta atttcccgcc tgctttttta tcaacgccag ttgcagtccc gccatccata
    58801 acctccagta atcctccctt ttcgatagcg gcaaagacgg caatcccatc tttttcaaca
    58861 attaagcgcc cgcctttaat cgtgggatca ttcgccgcg
//
LOCUS       DAZOYL010000002        44714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZOYL010000002 DAZOYL010000000
VERSION     DAZOYL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.002"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(308..850)
                     /gene="ycfP"
                     /locus_tag="ACHUOY_000053"
     CDS             complement(308..850)
                     /gene="ycfP"
                     /locus_tag="ACHUOY_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR0680956.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(873..1898)
                     /gene="nagZ"
                     /locus_tag="ACHUOY_000054"
     CDS             complement(873..1898)
                     /gene="nagZ"
                     /locus_tag="ACHUOY_000054"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR0680957.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1909..2733)
                     /gene="thiK"
                     /locus_tag="ACHUOY_000055"
     CDS             complement(1909..2733)
                     /gene="thiK"
                     /locus_tag="ACHUOY_000055"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR0680958.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2714..3355)
                     /gene="lpoB"
                     /locus_tag="ACHUOY_000056"
     CDS             complement(2714..3355)
                     /gene="lpoB"
                     /locus_tag="ACHUOY_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR0680959.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVKAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3369..3746)
                     /locus_tag="ACHUOY_000057"
     CDS             complement(3369..3746)
                     /locus_tag="ACHUOY_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR0680960.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3749..4108)
                     /gene="hinT"
                     /locus_tag="ACHUOY_000058"
     CDS             complement(3749..4108)
                     /gene="hinT"
                     /locus_tag="ACHUOY_000058"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR0680961.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4442..6631
                     /gene="fhuE"
                     /locus_tag="ACHUOY_000059"
     CDS             4442..6631
                     /gene="fhuE"
                     /locus_tag="ACHUOY_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR0680962.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(6691..8124)
                     /gene="ptsG"
                     /locus_tag="ACHUOY_000060"
     CDS             complement(6691..8124)
                     /gene="ptsG"
                     /locus_tag="ACHUOY_000060"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR0680963.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8419..9216)
                     /locus_tag="ACHUOY_000061"
     CDS             complement(8419..9216)
                     /locus_tag="ACHUOY_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0680964.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9227..10231)
                     /gene="holB"
                     /locus_tag="ACHUOY_000062"
     CDS             complement(9227..10231)
                     /gene="holB"
                     /locus_tag="ACHUOY_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR0680965.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10228..10869)
                     /gene="tmk"
                     /locus_tag="ACHUOY_000063"
     CDS             complement(10228..10869)
                     /gene="tmk"
                     /locus_tag="ACHUOY_000063"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0680966.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10859..11881)
                     /gene="yceG"
                     /locus_tag="ACHUOY_000064"
     CDS             complement(10859..11881)
                     /gene="yceG"
                     /locus_tag="ACHUOY_000064"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR0680967.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11884..12693)
                     /gene="pabC"
                     /locus_tag="ACHUOY_000065"
     CDS             complement(11884..12693)
                     /gene="pabC"
                     /locus_tag="ACHUOY_000065"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR0680968.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12813..14054)
                     /gene="fabF"
                     /locus_tag="ACHUOY_000066"
     CDS             complement(12813..14054)
                     /gene="fabF"
                     /locus_tag="ACHUOY_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR0680969.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14142..14378)
                     /gene="acpP"
                     /locus_tag="ACHUOY_000067"
     CDS             complement(14142..14378)
                     /gene="acpP"
                     /locus_tag="ACHUOY_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0680970.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14589..15323)
                     /gene="fabG"
                     /locus_tag="ACHUOY_000068"
     CDS             complement(14589..15323)
                     /gene="fabG"
                     /locus_tag="ACHUOY_000068"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR0680971.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15336..16265)
                     /gene="fabD"
                     /locus_tag="ACHUOY_000069"
     CDS             complement(15336..16265)
                     /gene="fabD"
                     /locus_tag="ACHUOY_000069"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR0680972.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16281..17234)
                     /gene="fabH"
                     /locus_tag="ACHUOY_000070"
     CDS             complement(16281..17234)
                     /gene="fabH"
                     /locus_tag="ACHUOY_000070"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR0680973.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17302..18372)
                     /gene="plsX"
                     /locus_tag="ACHUOY_000071"
     CDS             complement(17302..18372)
                     /gene="plsX"
                     /locus_tag="ACHUOY_000071"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR0680974.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18453..18626)
                     /gene="rpmF"
                     /locus_tag="ACHUOY_000072"
     CDS             complement(18453..18626)
                     /gene="rpmF"
                     /locus_tag="ACHUOY_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR0680975.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18678..19199)
                     /gene="yceD"
                     /locus_tag="ACHUOY_000073"
     CDS             complement(18678..19199)
                     /gene="yceD"
                     /locus_tag="ACHUOY_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR0680976.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            19398..19982
                     /gene="yceF"
                     /locus_tag="ACHUOY_000074"
     CDS             19398..19982
                     /gene="yceF"
                     /locus_tag="ACHUOY_000074"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR0680977.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20094..21053)
                     /gene="rluC"
                     /locus_tag="ACHUOY_000075"
     CDS             complement(20094..21053)
                     /gene="rluC"
                     /locus_tag="ACHUOY_000075"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR0680978.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21626..24811
                     /gene="rne"
                     /locus_tag="ACHUOY_000076"
     CDS             21626..24811
                     /gene="rne"
                     /locus_tag="ACHUOY_000076"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR0680979.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25006..25959)
                     /gene="flgL"
                     /locus_tag="ACHUOY_000077"
     CDS             complement(25006..25959)
                     /gene="flgL"
                     /locus_tag="ACHUOY_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR0680980.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25971..27614)
                     /gene="flgK"
                     /locus_tag="ACHUOY_000078"
     CDS             complement(25971..27614)
                     /gene="flgK"
                     /locus_tag="ACHUOY_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR0680981.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27680..28621)
                     /gene="flgJ"
                     /locus_tag="ACHUOY_000079"
     CDS             complement(27680..28621)
                     /gene="flgJ"
                     /locus_tag="ACHUOY_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR0680982.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28621..29718)
                     /gene="flgI"
                     /locus_tag="ACHUOY_000080"
     CDS             complement(28621..29718)
                     /gene="flgI"
                     /locus_tag="ACHUOY_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR0680983.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29730..30428)
                     /gene="flgH"
                     /locus_tag="ACHUOY_000081"
     CDS             complement(29730..30428)
                     /gene="flgH"
                     /locus_tag="ACHUOY_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR0680984.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30481..31253)
                     /gene="flgG"
                     /locus_tag="ACHUOY_000082"
                     /pseudo
     CDS             complement(30481..31253)
                     /gene="flgG"
                     /locus_tag="ACHUOY_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(31425..32180)
                     /gene="flgF"
                     /locus_tag="ACHUOY_000083"
     CDS             complement(31425..32180)
                     /gene="flgF"
                     /locus_tag="ACHUOY_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR0680985.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32200..33405)
                     /gene="flgE"
                     /locus_tag="ACHUOY_000084"
     CDS             complement(32200..33405)
                     /gene="flgE"
                     /locus_tag="ACHUOY_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR0680986.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33430..34125)
                     /gene="flgD"
                     /locus_tag="ACHUOY_000085"
     CDS             complement(33430..34125)
                     /gene="flgD"
                     /locus_tag="ACHUOY_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR0680987.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34137..34541)
                     /gene="flgC"
                     /locus_tag="ACHUOY_000086"
     CDS             complement(34137..34541)
                     /gene="flgC"
                     /locus_tag="ACHUOY_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR0680988.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34545..34961)
                     /gene="flgB"
                     /locus_tag="ACHUOY_000087"
     CDS             complement(34545..34961)
                     /gene="flgB"
                     /locus_tag="ACHUOY_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR0680989.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35116..35775
                     /gene="flgA"
                     /locus_tag="ACHUOY_000088"
     CDS             35116..35775
                     /gene="flgA"
                     /locus_tag="ACHUOY_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR0680990.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            35851..36144
                     /gene="flgM"
                     /locus_tag="ACHUOY_000089"
     CDS             35851..36144
                     /gene="flgM"
                     /locus_tag="ACHUOY_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR0680991.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            36149..36565
                     /gene="flgN"
                     /locus_tag="ACHUOY_000090"
     CDS             36149..36565
                     /gene="flgN"
                     /locus_tag="ACHUOY_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR0680992.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36605..38140)
                     /gene="murJ"
                     /locus_tag="ACHUOY_000091"
     CDS             complement(36605..38140)
                     /gene="murJ"
                     /locus_tag="ACHUOY_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR0680993.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38250..39173)
                     /locus_tag="ACHUOY_000092"
     CDS             complement(38250..39173)
                     /locus_tag="ACHUOY_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0680994.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39175..39822)
                     /locus_tag="ACHUOY_000093"
     CDS             complement(39175..39822)
                     /locus_tag="ACHUOY_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR0680995.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39833..40417)
                     /gene="rimJ"
                     /locus_tag="ACHUOY_000094"
     CDS             complement(39833..40417)
                     /gene="rimJ"
                     /locus_tag="ACHUOY_000094"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0680996.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40653..41861
                     /gene="mdtH"
                     /locus_tag="ACHUOY_000095"
     CDS             40653..41861
                     /gene="mdtH"
                     /locus_tag="ACHUOY_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR0680997.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            41925..42572
                     /gene="grxB"
                     /locus_tag="ACHUOY_000096"
     CDS             41925..42572
                     /gene="grxB"
                     /locus_tag="ACHUOY_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR0680998.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42706..43266
                     /locus_tag="ACHUOY_000097"
     CDS             42706..43266
                     /locus_tag="ACHUOY_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0680999.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43372..44418
                     /gene="pyrC"
                     /locus_tag="ACHUOY_000098"
     CDS             43372..44418
                     /gene="pyrC"
                     /locus_tag="ACHUOY_000098"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR0681000.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44492..>44714
                     /gene="dinI"
                     /locus_tag="ACHUOY_000099"
     CDS             44492..>44714
                     /gene="dinI"
                     /locus_tag="ACHUOY_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR0681001.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESA"
BASE COUNT        10819 a        12099 c        11139 g        10657 t
ORIGIN      
        1 gagaataaca tgaatggtgc attggtcata ccaaaattga tgttaatcaa gttttgttgc
       61 tgaaaagata ggcgacttta ccgggtgaga tcaaaaaaca gagtttattg tttattattt
      121 gagcaagtcc ggaagggtga tatagggtga cgcgtatatc gagggcgttt ggtcgtcctg
      181 ataagatgcg cagcatcgca tcaggcattg atgctcgact gtcggatgcg gcgtaaacgc
      241 cttatccgac ttacagaatt cggagcgcat caggtaccgg caaggttacc tgatgcgacg
      301 agggcattta cccgagggtt ttgaacgcct taatgcgctg caaatgcggg gagatattct
      361 tgaatttgtg cgtctgctct tcgtcccaga caatctcgta ataatgatgc aactcttcag
      421 atgtccgctg gctgttaagc gcttcatcat tacgcgacaa aatcaccagg caacgatcgc
      481 gattcttctc gcggaagttg gtcacacact tagtggcaat atcggcatac tcttccgggc
      541 gatcaatctt gccttccatg ttctcataag ggaacaaatt agggttgaag atcacctggc
      601 ggatatcgca aagaaaacca atccgttccg cccagtatcc gcccagacca acgccgcaaa
      661 tcagcggacg ctcgtcgacg ttcagttgca acattttgtc cacttctttg agcagatgct
      721 gcatatcatg tttcggatgc cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa
      781 actgcaattg taagactttc tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat
      841 agataatcat cgcttcctca cataagccag ggttagtgac ctgctttctc ttcctgccag
      901 cgttcatgta actgattcag acgggtgctg atcgccttcc agcgagccga gtccatcagt
      961 tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa cacgttctgc cttgatcggt
     1021 gacagattat ctaacacgct gacggcccct ttacgattat tgcagaccag gatcatatcg
     1081 caacctgcgt ccagtgatgc ctgcccgcgt tcggcataac tgcccataat cgcggcacct
     1141 tccatcgata aatcgtcaga gaaaattaca ccgtcaaaac acagttcctg acgcaaaacg
     1201 gttttcagcc agtaggaaga accgctcgcc ggacgcggat caacatcact gtagatcaca
     1261 tgcgcaggca taatggcgtc gagtttattt tcgcggatta aggaactgaa gaccgacata
     1321 tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg gtgtttcttt gtgtgagtct
     1381 gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc
     1441 ataccatcaa taaaccggct ggcaattgcc agggcttttt gtggatcggc atgataagaa
     1501 cgctcgccaa tcgccgcgct gatatgcccg acatccagca ctggcgcaaa gctgatatca
     1561 atatccatag cgatcatttc gctggccatc agccaacccg cctcttgcgc cagtttgccg
     1621 ccctcttcca ttcccaacag cgcagcaaag gattgtgctg ccggtaagcg ggtaaaacct
     1681 tcgcgaaaac gctgcacgcg tccaccttcc tgatctaccg ccaccaccag atgattgcgc
     1741 gatgctgcgc ggatctggcg caccagttca cgtaactggg caggatcatg atagttacgc
     1801 gtaaagagaa tcagccctcc caccagcgga tgcgccagta tttcacgctc ttccgcgtcc
     1861 agttcgtaac cttcgacatc caacattact ggacccacac tgctctcctt attgttttat
     1921 taatagctgc cgccaggtgt catcggccag cctgataaat tgttgatcgc cgatttgtcg
     1981 ccagcggtac tcaaaccacc ctgctttgag catcagcagc cagggaaacc atcgcctgac
     2041 ctgacgccat aattgcgccg gataaatctt cgcgcgagtg gcatagtcat tgaccaattg
     2101 ccggtgctgt tcagtatttt ccacccacac cgccgccagt tccagcgcga tatcaccatc
     2161 tccggcatac tcccagtcga tgagttttaa ccctgacgcg ctatgcacta aatttccggc
     2221 gtggacatcc atatgcaatg gacttaagcg taaaggccgt ggttcccgcg ctttgcgcag
     2281 acgttttaac attcgcagcc aacccacagt ccgccgcgcc ggatcgcttt gctgccagta
     2341 cagttccagt aagggcaaca gcgttattcg ccagccaaaa cgtggttgtt gatgaagata
     2401 atacagcaag cctgccagtt cgttggtatc cggcaaatac gtttttaccg cgccgggcag
     2461 atagtcgact accatccagt cacggagata taaatgcggc ttcggtgcaa tgcttgcggg
     2521 tagttgtgat aaagcccgat actggcgcaa gaacgcggac tgcggcgcat caggatcgtg
     2581 cggctgacgc acaacaaaac gctggccctg atgttcaatg agaaaactac cgccactaag
     2641 cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc
     2701 gcgcgtgatg ggattattgc tgcgaaacgg cacctttacc tgaccagata atttcgcccg
     2761 tctgcaccag catcagctgc atttgtaggg tcggagcctt aacgttgcca gaggcgctgg
     2821 agtacagcac gtaatgagcg ccgacattgc gggcaatgcc tatggcttta ctacgggtac
     2881 ctaaactgtc ctgcggcgac aaacctaact gttgcttagc catcgacagc tgctgggcgg
     2941 aaaccagggt aaatttcccg ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg
     3001 cggcattcag cgaaccgtta gtacggttgt taacgctatc aaccagcagg acgctacccg
     3061 cagtcacccc gtcagcccca agcatcttac tgaccatcgg ctgcattgcg ccattccagt
     3121 cataatggcg aatatgcggc gcaggcggtg ccgtttgatc ttcgtgctca attgggcctg
     3181 gctgctgcgg gatcgtcggc accgagggca ctgtggggac aggctgttgt ggctcggctg
     3241 gttgttccgg cgctggtttc acttcttcta ccggtgcagg ttcacgttgc cccacacacc
     3301 cggcgagaaa catcgccagc gcggtaatca aggcgtagcg actcattttt gtcatcaaga
     3361 ttcacccctt acaaatatag ataaagtctg actttgtgcg cccccagaaa attggcgctg
     3421 ccgtacagcg ttaccgccga atgtgcggga atgctaacgc tgcgtggcct ttccagagga
     3481 tgcatctcca gccctctggc gtcataccag taaaaacgat aatgaacggt aacgggttct
     3541 tgcctttcgt tataaagcgt tgaggatgct gaaggttgaa tatcagacgt cgaaaggacg
     3601 ggcttttctg cactgatgcc cgcagccaat aaagatgact ccatcaccag cgattgctca
     3661 tcattcaccg gaatttccgg atgtgaacga cagcccacca gcagtaacaa cgccagagac
     3721 accagcccaa agcatccttt tctcatcgtt acagaccttt atgcgccagc attggtccca
     3781 gcggacggcc acccaacaag tgcatatgga tgtggtaaac ctcttgtccg ccatggcggt
     3841 tggtattcat gatcagacga tagccatctt cggcaatacc ttcttgctca gcaatttttg
     3901 ccgctacggt gatcatgcgt cccagcgcct gctcatgctc agctgacacg tcgttcacag
     3961 tcggaatgag gatattcgga atgatcagaa tatgcgttgg cgcttgcggc gaaatatcgc
     4021 gaaacgccgt taccagatca tcctggtaga cgatatcgga ggggatctca cgacgaataa
     4081 ttttgctgaa tatagtttct tctgccacga cattttcctt tttcaaatag ccatgcgttg
     4141 cgccatgcta cgccgcgcca cactgcgagt atagagtatg gtcgagttaa acctgctctt
     4201 tcaaccttaa ctcacgattt cttaagcaaa aaatcgtttt gctgccgtat atatcgccat
     4261 tattcccatt tctgcctgcg aatgcgctga aaagacattc tctgtttggt ttacacgaca
     4321 aatatgaatg cgcatatttc tcatttgcat ttacaaacaa aattattcgc acactaaata
     4381 taaccgctca tcaccctcct ccggcatgag cctgacgaca acataaacca agagatttca
     4441 aatgctttca acacaattta acagggataa tcaatatcaa gccatcacca aaccgtcact
     4501 acttgccggt tgcatagcac tggcactatt accttctgcc gcttttgctg caccagccac
     4561 tgaagaaacg gtgattgttg agggttcagc cacagctcca gatgatggcg aaaatgatta
     4621 cagcgtaacg tctacctctg cgggtaccaa aatgcagatg actcaacttg atattcctca
     4681 gtcggtcact attgttagcc agcagcggat ggaagatcag cagttacaaa cgctgggcga
     4741 agtgatggaa aacacgctgg ggatcagcaa aagtcaggcg gattccgatc gtgctcttta
     4801 ttattcccgt ggattccaga tcgataacta tatggttgat ggtatcccca cctattttga
     4861 atcgcgctgg aatctgggcg acgcactttc tgatatggca ctctttgaac gcgtagaagt
     4921 agtgcgtggc gcgacaggac tcatgaccgg gacgggtaat ccatctgcgg caattaatat
     4981 ggttcgaaaa cacgcgacca gtcgtgaatt taaaggcgat gtctcggcgg aatacggtag
     5041 ctggaacaaa gaacggtatg tggcggattt acaaagccca ctcaccgaag acggtaaaat
     5101 ccgcgcgcga attgtcggcg gctaccagaa taacgactca tggctggacc gctacaacag
     5161 tgaaaagacc ttcttctcgg gcattgtcga tgctgattta ggcgatctta cgacgctttc
     5221 agccggttac gaatatcagc gcattgatgt taatagccca acctggggcg gtttaccgcg
     5281 ctggaatact gatggcagca gcaacagtta cgatcgtgca cgcagtaccg cacctgactg
     5341 ggcgtacaac gataaagaga tcaacaaggt ctttatgacc ctgaagcagc ggtttgctga
     5401 tacctggcaa gcgacactga atgccaccca ctctgaagtc gaatttgaca gcaaaatgat
     5461 gtatgtcgat gcctatgtaa acaaagcgga tggtatgctg gttgggccat acagtaatta
     5521 tggacctggc tttgattatg tcggcggcac cggttggaac agtggcaaac gtaaagttga
     5581 tgcgctggat ttgttcgctg acggtagtta tgaattgttt ggtcgtcagc acaatctaat
     5641 gtttggtggc agttacagca aacaaaacaa tcgttacttc agttcatggg ccaacatctt
     5701 cccggatgaa attggcagtt tctacaactt taatggcaat ttcccacaaa ccgactggtc
     5761 accacagagc ctggcacagg acgataccac acatatgaaa tcgttatatg ctgctactcg
     5821 agtcaccctt gccgatccgc tgcatctgat cctcggcgca cgttatacca actggcgggt
     5881 tgatacgctg acttacagca tggagaaaaa ccacaccacg ccttacgctg gtctggtgtt
     5941 tgacatcaat gacaactggt cgacctacgc cagctatacc tctattttcc agccgcaaaa
     6001 tgatcgtgac agttcaggca aatatctggc tccaatcacc ggtaacaact acgagctggg
     6061 tctgaaatcg gactggatga atagccgtct gaccaccacg ttagccatct tccgtattga
     6121 gcaggataat gtcgctcagt ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc
     6181 ctataaagcg gtggatggga cagtcagtaa aggggtggaa tttgaactca acggcgcaat
     6241 taccgacaac tggcagctga catttggcgc aacgcgctat attgcagagg ataacgaagg
     6301 aaacgccgtt aatcctaatc tgccacgcac cacggttaaa atgttcacca gctatcggtt
     6361 gcctgtcatg ccagagttga cagtcggcgg tggtgttaac tggcaaaatc gcgtgtatac
     6421 cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa ggtagctacg cgctggtgga
     6481 tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta caggggaacg tcaataacct
     6541 gttcgacaaa acctacgata ccaacatgga aggttctatc gtctacggcg caccgcgtaa
     6601 tttcagcatt accggcacgt atcaattctg aataacacct gtaaaaaagg cagccatctg
     6661 gcggccttag tctccccaac gtcttacgga ttagtggtta cggatgtact catccatctc
     6721 ggttttcagg ttatcggatt tagtaccgaa aatcgcctga acaccagaac cagcaaccac
     6781 tacgcccgct gcgcccagtt tcttcaggcc agcctgatcc actttagaca catcagcaac
     6841 gctgacgcgc agacgggtaa tacatgcgtc gaggttagta atgttttctt taccaccaaa
     6901 tgcagcaacc agagccggtg ccatttcgct ggtacctgtt gcttttgcat cttcagtcgc
     6961 gtcttcacga cccggcgttt tcagatccag tgctttaatc agcacgcgga agatggtgta
     7021 gtaaacaatc gcataaccga taccgacgat cgggaacagc cacagtttgc tgctgttacc
     7081 agacagaacg ataaagtcga tcagaccgtg cgagaacgac gtaccgtcac gcatccccag
     7141 aagaatacag attgggaatg ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg
     7201 cgcaacgaac atgaaggaga actcgatcgg ctcggtgata ccggtcagga acgaggtcag
     7261 cgccgcggag atcataatac cgcccacttt cgcgcggttt tctggtttag cagagtgcca
     7321 gatagcaatt gcggcagctg gcagaccgta cattttgaac aggaagccac cagacagttt
     7381 acccgcagtc gggtcacccg ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc
     7441 tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg ttccagatgt ggtgcagacc
     7501 aaacggcacc aggcaacgtt cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg
     7561 gtaagcagcc cactgagaga aggtctggat tgcagaacca atcggcggcc aaatgaagga
     7621 cagcacaacg ccagtaaaga tggcagccag gccagaaatg atcggcacaa agcgtttacc
     7681 ggcaaagaag ccaagatact caggcagctt aatacggtag aaacggttaa acatgtacgc
     7741 tgcgatcgca ccggagataa tccctccgag tacgccagta tccgccaggt gtttagaggc
     7801 gatttcttca gcaggtaaat gcagtaccag tggcgcaacc acggccatgg ttttaaccat
     7861 gatgccatag gcaacaactg cggccagcgc ggatacgcca tcgttattgg taaagccgag
     7921 ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc
     7981 cataacatgc gatacaacgg cgggcagcca gctgaaattc gcggaaccga cgcccagcag
     8041 aatacctgcg ataggcagta cggataccgg cagcatcagc gatttaccga ccttttgcag
     8101 gttagcaaat gcattcttaa acataattga gagtgctcct gagtatgggt gcttttttta
     8161 cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta cagggcatct aagcgccctt
     8221 tatttattac acagagtaaa ataattcagt gccgatttgt ttgacggcta tcacatttca
     8281 acttcagatc attggagaag ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac
     8341 gtggttattc accgcccaat tttcggcggt gaactttact cgttttagcc attaattacg
     8401 agctttaaaa aaactcattc aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag
     8461 acgggcgaag ttatcggtgg ttacctgcgc cagttcttca acggcaacac ctttcaacac
     8521 agccatgtat tctgcaacgt cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg
     8581 taccggcgca aggtaaggtg agtcagtttc caccagtaac cgatccaggg ggacataacg
     8641 cgcagcatct cgaagttgct ccgcattacg gaaggtcaca atgccggaaa aggagatgta
     8701 aaatccgaga tccagtaatt tacccgccgt ttctctgtcc tctgtaaaac agtgtagtac
     8761 gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc agcgtatcgg cgcgggcgtc
     8821 acgggtatgg acgataaccg gcttgttcag ttcacgacca atctggatat gatggatgaa
     8881 ggactcttgc tgacgaactt tagtttccgg tgtgtaataa taatccagcc cggtttcacc
     8941 cagcgctaca acaccctctt ctgccgccag acggcgtaaa tcttctacat cgtagggatc
     9001 attctggttg agtggatgca cgccacaaga aaatacgacg ttgtcacgtt cgcctaccag
     9061 atcccgcata tgtaaataac ccggtaatgt tgtggcgact gccagacaaa atttcacatc
     9121 gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta tgcaaagatt cataatccag
     9181 accatcgaga tggcagtgtg agtcgactaa aaacatgatg tctctcttaa agatgaggaa
     9241 ccggtagcac aacgcccggt tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga
     9301 gaagctcgcg gttgatgcct gtgacagaca ttaactgttc acgaatgtgg caaacatccc
     9361 ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc cagttcgacg acaaggcccg
     9421 gcacatcaac attggtcacc tgcgcagcac catgatggcg ttttagcgca tccatcagca
     9481 acgttgccag ccagtgtaaa cgcgccggag cttgttcatg attaagggcc gccagcagcg
     9541 aataccagtc gcccgatggc acgctatatg ccaatgcctg acacaatgtt tcacgagcct
     9601 gccagttatc tccctgaaac aacgccagtg ccgcgccagg cgaaccggcg cttaagcgca
     9661 atgcggcaag taatgcatcc tgtgacattg tcacttcgcg tgaaagccag gtcacggcgt
     9721 actgttccgg cggcggcgca aggtaatgta accgacaacg actacgtaat gttgccagta
     9781 aacgttcagg ctcgcgggta gccaggaaaa accaggtttc tgctggtggc tcttcaagcg
     9841 ttttcagcaa tgcgttagcc gcggcgtcgg tcagtaaggc agcatcggtt acccagacaa
     9901 ctttcgcacc acctaggcgt gcgtgctcat tcagcttttc ggtgacctca cgtactgcat
     9961 caatgcccag cgtatttttc cctttttcgg gagccagggt gtagtaatcg ggatgcgtgc
    10021 cagcctgcat caactgacat ccacgacagt gaccgcaact tttgtggccc tgcggttgtt
    10081 ggcagagtaa ataacggctc agggcgtaga ttaaagcatc atcgcccatg cccggtaacg
    10141 cctgaatgag tagcgcatgg tgacctcttc cggcctgata gctggctacc agtttttcga
    10201 aatcaggtcg taaccatgga taccatctca tgcgtccaac tccttcaccc agtgggtcac
    10261 ggtagtgcgg attgcatcca tcacggcctc cagcggctgg gtggcatcaa tggtatgaat
    10321 acttttatct tgtgcagcca gttccagata gcgggcgcgg gtgcgattaa agaaatcgaa
    10381 agactcttgc tcaatacgat ccagctcgcc gcgcgcacgc gcgcgtttta agccaacttc
    10441 cggggtaaca tcgagataga gcgttaagtc ggggcgaaaa tccccgagaa cagcatcacg
    10501 cagtgttgcc agcatatgtt ggtcaatacc acgtcctccg ccctgatacg cctgagtgga
    10561 gagatcgtgg cgatcgccaa tcacccaggt gccgttagcc agagctggtt tgatgaccgt
    10621 ttctaccagt tgaacgcgcg cggcataaaa catcagaact tcggctttat cggtaatgac
    10681 ttcatcgcct accgatttga tatccagcac caggcttctt aacttttcgg caagttgcgt
    10741 accgccaggt tcccgagtga aaaccatgtc gcggataccc agttgctcaa gcgtctcaac
    10801 caccacatta cgcgcggtag ttttgcctgc gccttccagc ccctcaatga cgatatactt
    10861 actgcgcatt tttttcctta agcactttca gataatcctg cacagacttg ttatggctgg
    10921 caagattggt attaaacgtg tgaccacctt taccatcggc cacaaaatag agatacggcg
    10981 tttttgccgg atgcgcagca gccttcagcg aatccgcccc cggcgtagct atcgcacccg
    11041 gcggcaaacc ggtaatggta taggtgttat acgctgtcgg cgtttccagg tctgcacgag
    11101 aaagtttgcc attataacgc tctcccatcc cgtaaatcac ggttgggtcg gtctgcaggc
    11161 gcataccaat gcgtaaacgg ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa
    11221 cggcggtttc tttttcgata attgatgcca tcgtcaccag ctgattttta tctttataag
    11281 gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgcg
    11341 ctcgcttgag taacgcgaca tcggtggtat tggcggtata catccaggtg tctggccaga
    11401 accaaccttc aatccactcc gggttttcca gttcaagtgc ctgcgctacg gtggcgtact
    11461 tatcgtcact cagcgtgtgc ttgatatacg gagcctcacg caattgcttg aggtagtcgc
    11521 tcagacgcat cccttctacc agtcgcagcg ggaactgtgc ttctttaccg ctttccagca
    11581 atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt
    11641 gagaaagatc cggttcgata cgcagcaacc attgaaaaac ccgtgggcga ttgatgatct
    11701 tatcggcata aagctgttca ccaagcgcca gacgtccggt ccctggcttc agggtaaata
    11761 tcgtctcttc tttgataagc aatttgctgt cggcaagatg gcgaaccttc cagacgccca
    11821 caccagcggc gatacccagt accaccagca ataacaagat aatcaataac acttttttca
    11881 tgactaattc gggcgctcac aaagtggggc taaatattca tataacgttg ctgacgaaaa
    11941 ggagacatcg ccacaggcac gtacgggcat cactggcatt aacgcattac aaataaccat
    12001 ctcatctgcc tgcaacgcct cttccagaga ggcttgcact tcgacaagct gataagagga
    12061 ttgtgccagc aaacggatac agaattgtcg cataatgccg ttaacacctg cctgatccag
    12121 acgcggcgta taaactacat tgcccttccg ccagaacaaa ttagccgcac agcattccgt
    12181 aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct caagatgaga
    12241 gcggatcaat acttgctcaa gccgattaag atgtttaata cctgcaagat gaggattgcg
    12301 ccccagccgc accgggctta gcgccaacgt catcccttcg ttacgcaaac ggtcgtaatg
    12361 tgcaggataa accgtaacgg agagaatccg cgttgccggt ccactgttca atgtgctgta
    12421 ccctcgcccg ccactaccgc gactgatcac gactttcagc acaccatttt gctgttctgc
    12481 tgccagcgtt ttcatctctt gttcaagctg aggccagaag tcacagaaaa tcatcaaccg
    12541 ctgacaagca tcctgtagtc gctggatatg cgccgataac aaactgactt taccgtcgat
    12601 aactctggcg gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc ttactgccag
    12661 cgattcctgc ttatgaccgt taattaagaa cataccggct ccttatcaga gaattgttag
    12721 tgtggcagca tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa
    12781 gcggaccttt tatatgggtg ggaaatgaca acttagatct ttttaaagat caaagaacca
    12841 ttagtgccac cgaagccgaa ggagttacac agcgtgtatt ccattccgct gacctgacgt
    12901 gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg ggttatccag gttgatggtt
    12961 ggcggaacag cctgatcgcg cagcgccagg atggagtaga tagattctac tgcacccgcc
    13021 gcacctaaca ggtgaccggt catagatttc gtggagctta ccaacacacg gcttgcagct
    13081 tcaccgaaga tggttttcac cgcctgcgct tcagctttat cgccagccgg cgtagaagta
    13141 ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc ctgcatcacg cagagcattt
    13201 gccatcgcca gagctgcgcc tgcgccattt tctggcggtg acgtcatatg ataagcatcg
    13261 ctgctcatac caaagccgac gagttcagcg taaattttcg caccgcgttt tttcgcgtgt
    13321 tcgtactctt caagtaccag cataccggca ccatcgccca gtacgaagcc atcacgctct
    13381 ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc
    13441 gcgccaaaac caccaacacc cagcggcgta ctggctttct ctgcgccacc tgcaaccatc
    13501 acgtcagcat cgccatacgc gataatacgc gcagcatggc caatgttgtg cacgccggaa
    13561 gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc catacatgat agtcagatga
    13621 cctgccacca tgttcacaat cgttgacgga acgaagaatg ggctgatctt acgtggacca
    13681 ccgttcatca gagatgtgtg gttttcttcg atcagtccga ggccgccaat cccggagcca
    13741 attgcggcac caatgcgggt tgcgttctct tccgttatat caaggccaga atcctgcatg
    13801 gcctgaacgc cagcgacaat tccatattga atgaaggcat ccatcttgcg ctgttctttg
    13861 cgcgagataa tgtcctcaca gttaaaatcc tttactaagc cagcaaattt cgttgcatag
    13921 gcgctagtat cgaaatggtc gattaggctg atgccactct gaccggcaag cagagctttc
    13981 caggtagact ctacggtatt gccgacagga gacaacatgc ccagtccggt cacaactaca
    14041 cgacgcttag acacgtttgt cctccaggga gggaaaaaat gattctagtg ggacaaaaag
    14101 ataaaactca ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg gtggccgttg
    14161 atgtaatcaa tggcagcctg aacggtggtg attttctcag cttcttcgtc cggaatctca
    14221 gtatcaaact cttcttccag agccattacc agctcaacgg tgtcaagaga atccgcgccc
    14281 aggtcttcaa cgaaagaagc attgttggta acttcttcct gcttaacgcc cagctgttcg
    14341 ccgataattt tcttaacgcg ttcttcgata gtgctcatac tcttaaattt cctatcaaaa
    14401 ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga aaaatttgca actaaatccc
    14461 ggcaggtctt accacgattt tacgttattt tgaggcctta ccccccaata acgcaaatca
    14521 ttttgcacta attattactc ataaccacgc agactgcgcg caacatgagc attttgtgca
    14581 aatcgcggtc agaccatgta catcccgccg ttcacatgca aagtttcacc cgtgatgtaa
    14641 gctgcttcgt cggatgccag gaatgcaacc gcgttggcga tttcctgtgc gccgccgagg
    14701 cgacccgcag gaacctgcgc caggataccc gcacgctggt catcgctcag cgcacgtgtc
    14761 atgtccgttt caataaagcc cggagcaaca acgtttacag taataccgcg tgacgcaact
    14821 tcgcgcgcca gtgatttact gaagccgatc aagcccgctt tcgccgcagc gtagttggcc
    14881 tgaccgccat ttcccatggt accaaccaca gaaccgatag tgataatacg accatgacgc
    14941 tttttcatca tagcgcgcat taccgctttt gacagacgga aaacagatga aaggttggtt
    15001 tcgataatat cgttccactc ttcatctttc attcgcatta acaggttatc acgagtgata
    15061 ccggcattat tgaccaggat atccacttca ccaaattctg cgcgaatttt ttccagaaca
    15121 gattcgatag atgccgggtc ggtcacattc aacatcagac ctttgccgtt ggcacctaaa
    15181 taatcgctga tcgcctgagc gccattttca ctggtcgcag tgccaataac tttcgcgcca
    15241 cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc ggttaccagt
    15301 gcgatttttc cttcaaaatt catgattttc ctcttttaaa gctcaagcgc cgctgccatc
    15361 gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt cgtcaggcca
    15421 gtaagcacct tgcccgggcc gacttcatag agatgttcta cgccttgcgc tgccatgtac
    15481 tcaacagact tcgtccactg aaccgggtta tacaactgac gtaccagtgc gtcacggatg
    15541 gcatcaccat tggtttcgca tttcacatca acgttattca caacagaaac tgttggtgcg
    15601 ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg ctgctggttt catcagcgca
    15661 cagtgagacg gtacgctcac tggtaacggc agagcacgtt ttgcgcccgc agctttacag
    15721 gcagcgccag cacgctcaac cgcttcttta tgaccggcaa taaccacctg tcccggagag
    15781 ttaaagttta ccggagaaac gacctgacct gctgcagctt cttcacacgc tttcgcaata
    15841 gacgcatcat ccagaccgat gattgccgcc atagcgcccg taccttccgg tacggcttct
    15901 tgcatgagct tgccgcgcat ctcaaccaga cgcaccgcat cagcgaaatc aatcacacca
    15961 gcgcaaacca gcgcggaata ttcccccagg ctgtgaccgg ccatcattgc cggtgcttta
    16021 ccgccctgct gctgccatac gcggtacagc gcaacagagg cagtcaacag cgctggctgg
    16081 gtttgccagg ttttattcag ttcttcagct ggcccctgct gggtcagcgc ccacaggtcg
    16141 tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga caattggata gctcgccgcc
    16201 atatcagcca gcattccaac ggtttgagaa ccctgtccag ggaacacaaa tgcaaattgc
    16261 gtcatgtttt aatccttatc ctagaaacga accagcgcgg agccccaggt gaatccaccg
    16321 ccaaaggctt caagcagaac caactgcccc ggcttaatgc gcccgtcgcg tacagcttca
    16381 tccagcgcgc acgggacaga ggccgcagag gtattaccgt ggcgatccag cgtcaccacg
    16441 acattgtcca tagacatacc gagttttttc gccgttgcac tgataatacg caggttagcc
    16501 tgatgcggaa ccagccagtc cagttgagaa cggtcaagat tatttgccgc cagcgtctca
    16561 tcaacgatgt gcgccagttc cgttaccgca accttgaaga cttcgttgcc cgccatcgtc
    16621 agatgaattg aattctctgg attcacacgg tcagcattag gcagcgtcag caactcacca
    16681 tagctaccgt cggcatgcag atgggtggag atgattcccg gctcttcaga ggcagccagc
    16741 accgcagcgc ccgcgccatc gccaaaaata ataatagtcc cacgatcggt tggatcgcag
    16801 gtgcgcgcca gtacatcgga accgacgacc agagcatact tcaccgcccc agatttcacg
    16861 tattgatcgg ctacgcttaa tgcataggtg aaacctgcgc aggctgctgc aacgtcaaat
    16921 gccgggcaac ctttaatgcc cagcatgctt tgaatctgac aagctgcgct cgggaaagcg
    16981 tgcgtagcag aagtcgttgc cacaacgatc aggccaatct ggtctttctc aatgcccgcc
    17041 atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc gtttggcgcg
    17101 gcaatgtggc gttcgcggat accggtacga gtgacaatcc actcgtcaga ggtgtccacc
    17161 attttttcca aatcggcgtt tgtccgcact tgttcgggca gatagctgcc agtaccaata
    17221 atcttcgtat acatgtacgc tcagtcactt ttcggttata taccgtcact tgcaaactgc
    17281 gagttcgctg gcagcgtcct gctaccgcag agttccgctt ttgccaccgt ccagcagctc
    17341 aaaaccagct gggtatacag attccaggcg agcggcaatt cgctgaggaa cttgtcgctg
    17401 caccgcctgc actgcctgtt caatcgcgac cgcaaaagct cgctgattgg ctgcaccatg
    17461 actttttatc accgtgccgc gcaatcctaa cagacaggcg ccgttatact ggtcggggtt
    17521 gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa cgctttaata acagtagcca
    17581 ccacgaccgt tttttccctt caccctgaga tttcagcaga gaaaggaaca tcctgacaac
    17641 accttccatc gtctttaatg tgacatttcc tgtaaagccg tcacaaacca gcacatctgt
    17701 cttgccagtt aacaactcat tggcttcaag atagccgata taattgatag aagggattgt
    17761 tttaagcacc gctgaggcat cccgaatact gtcgagaccc tttacttctt cttcaccaat
    17821 attgagcaac gccacgcgag gattgggaat ttccaccacc tcttcagcca gaactgagcc
    17881 cataatggca aattgcacca acattgtact gtcacaatcg acgttggccc ctaagtcaag
    17941 gaccaccgtt ttgccctttt gctgatgtgg taataccgtc accagcgccg gacgctcaat
    18001 cccctccagg ggcttgagta ataattttgc cagccccatc agtgccccgg tattaccggc
    18061 actgacacag gcttgcgctc gaccttcttt caccagctcc agggccacgc gcattgaact
    18121 cccacgactg gcgcggatag cttgcgaagg ccgggcatca ctggcgataa ctgactgcgc
    18181 aggaataatc tgcagacgcg aacgttgttc aaagtcagct ttagcaagta atggcgtgat
    18241 ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa ttagaattca gtgcctgcaa
    18301 tgctgcaggc actgtcacgg aagggccaaa atcccctccc atgacatcta acgccagggt
    18361 tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc ctggggaaaa
    18421 tcctcactaa gcttcatcac gcagacgcgt gattacttag cgatgacctt gcggccgcgg
    18481 tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt caccagaagt tttgtctaca
    18541 gacaggctgg tgactgcggt cagcgcgtca tgggaacgac gcatgccacg tttggaacgg
    18601 gttggtttat tctgttgtac ggccatggac cttactcctc aattacggtt tatccccgat
    18661 ccaacgggga gcaccaatta cttacgcttt aagctggcta atacggcaaa tgggtttggc
    18721 ttttgcactt cttcaggcag ttcaccaaag accatgtccg cttcggacac ttcacagtgt
    18781 tcagaatcat gcaccggaac taccggcaag gcgaggatga tttcatcttc aaccattgca
    18841 agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc cggcagtgct
    18901 tcagcctgtt cgtctgaacg cacaggacta aaacaatacg ttgtgtagac ctgatgagta
    18961 aacggcttcc cgcaacgctg acactcgagc gttaccgtca ccttcgcatc gccgtttaac
    19021 actgcgagac gttggttatc gatagcgaac gacatggagc attccacatc actgtccaca
    19081 ctgactacgg attcggcgac gcgctcaacc tgatcagggg tatagatacc ctggtaatca
    19141 aggcgttttt gagccgtacg aaccggatcg agagtcaggg gtaattttac cttttgcata
    19201 gggcgcgcat attaactttg taacgtcata gagtcaaaga aaaaggcagc ctgtggttgc
    19261 cttttgccaa taattcgcac acattgcggg ttactgcttt attttcgttc agtgctgata
    19321 acgaacatca acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc aatacgctat
    19381 atctgatgga aaaaaatatg cctaaactta ttttagcctc cacctcgcca tggcgtcgcg
    19441 ctcttctgga aaaactgcaa atctcttttg aatgtgcagc accagaggtc gacgaaaccc
    19501 cacgcagcga cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa aaagcgcaat
    19561 ctctggcgtc acgttatccg gatcatttaa ttattggttc agaccaggta tgtgttcttg
    19621 atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc ccgtctgcaa ttacgcaaag
    19681 ccagcggcaa tatcgtcacc ttctatactg gactggcgtt gtttaattcg gcgaatgggc
    19741 atctacaaac agaagtggag ccttttgacg tccatttccg tcacctgagc gaggcggaga
    19801 ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc gggtagcttt aagagtgaag
    19861 gatttggcat tacgctgttt gagcgtttag agggacgtga ccctaacacg ctggttggtt
    19921 taccgctaat cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg ctgatgggat
    19981 agttgtttgc gctggatgcg gttacgtcgc atccggcaat cggtgcaggc ggcctgatgc
    20041 ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc ttatatgctt gtattagcgc
    20101 gcgttacgca gcttttgcaa acaacgcttc aaaccttcat ccatcggcgc ttcgatacgc
    20161 atcacctcac cggtccccgg atgggtaaac ttcaacgctg cggcgtgcag gaacagacga
    20221 tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt cacggtcacc gtagcgatcg
    20281 tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac ggatctgatg agtacgtccg
    20341 gttaccgggc tacaacgcac cagggtggca aatgcatagc gttcttccac tttaaagcgt
    20401 gtttccgacg gtttgccttc ctgactgaca cgcacgatac gttcgccgct ttgcagaata
    20461 tttttcaata acggcgcttg aacgctcttc acatgcgact gccactgacc gcgcaccagc
    20521 gccaggtaat ctttttgcat tcctttttca cgtaattgct catgcagaga acgcaacgcc
    20581 gagcgttttt tcgctatcag caacacacct gaagtgtccc ggtcgagacg atgaaccagt
    20641 tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt cgataacgcc gaagcttaaa
    20701 ccactgccgc catgtaccgc cgtaccggaa ggtttattca gcaccaggat atgatcatct
    20761 tcatataaga tgacgtccgc cagcgccgcc accttttgca gatgtggcga aaccgcctct
    20821 tcttcccgct cagcaacgcg aaccggtggg atacgcacct catcacccgc ttcgagttta
    20881 tattcaggct taatacgttt tttgttcacc cgcacttcgc ctttacgcaa aatacggtaa
    20941 atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa agttatcgat acgttgcccc
    21001 gcttcgtcag cggtgatagc aacaattttt acggatggag tctctgtttt catggtgggc
    21061 gattctaatt agccaacagg attcgcgcca ctcatttttc tatgcttata tttagttttg
    21121 caccttatta cttcactacg tgatcacttt attgatggtt attaaaccaa tcaccagcaa
    21181 gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa aagtcatctt gctataacaa
    21241 ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc ttgttaaaac aagaaatttt
    21301 acggaataac ccattttgcc cgaccgatca tccacgcagc aatggcgtaa gacgtattga
    21361 tctttcaggc agttagcggg ctgcgggttg cagtccttac cggtagatgg aaatatttct
    21421 ggagagtaat acccagtctg tttctttgat aattgcgctg tttttccgca tgaaaaacgg
    21481 gcaaccgaca ctctgcgcct ctttgagctg acgataaccg tgaggttggc gacgcgacta
    21541 gacacgaggc catcggttca cacccggaaa ggcgttactt tgcccgcagc ttagtcgtca
    21601 atgtaagaat aatgagtaag ttacgatgaa aagaatgtta atcaacgcaa ctcagcagga
    21661 agagttgcgc gttgcccttg tagatgggca gcgtctttat gacctggata tcgaaagtcc
    21721 agggcacgag cagaaaaagg caaacatcta caaaggtaaa atcacccgca ttgaaccgag
    21781 tctggaagct gcttttgttg attacggcgc tgaacgtcac ggtttcctcc cactaaaaga
    21841 aattgcccgc gaatatttcc ctgctaacta cagtgctcat ggtcgtccca acattaaaga
    21901 tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat aaagaagagc gcggcaacaa
    21961 aggcgcggca ttaaccacct ttatcagtct ggcgggtagc tatctggttc tgatgccaaa
    22021 taacccgcgc gcaggtggca tttctcgccg tatcgaaggc gatgaccgta ccgaattaaa
    22081 agaagcactg gcaagccttg aactgccgga aggcatgggg cttatcgtgc gcaccgctgg
    22141 cgtaggcaaa tctgctgagg cgctgcaatg ggatttaagc ttccgcctga aacactggga
    22201 agccatcaaa aaagccgctg aaagccgccc ggctccgttc ctgattcatc aggagagcaa
    22261 cgtaatcgtt cgcgcattcc gcgattactt acgtcaggac atcggcgaaa tccttatcga
    22321 taacccgaaa gtgctcgaac tggcacgtca gcatatcgct gcattaggtc gcccggattt
    22381 cagcagcaaa atcaaactgt ataccggcga gatcccgctg ttcagccact accagattga
    22441 gtcgcagatc gagtccgcct tccagcgtga agtccgtctg ccgtccggtg gttcgattgt
    22501 tatcgacagc accgaagcgt taacggccat cgacatcaac tccgcacgcg cgacccgcgg
    22561 cggcgatatc gaagaaaccg cgtttaacac caacctcgaa gctgccgatg agattgctcg
    22621 tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc gacttcatcg acatgacgcc
    22681 tgtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa gcggtgcgtc aggaccgtgc
    22741 gcgtattcaa atcagccata tttctcgctt tggcctgctg gaaatgtccc gccagcgcct
    22801 gagcccatca ctgggtgaat ccagccatca cgtctgcccg cgctgctccg gtaccggtac
    22861 cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt ttgatcgaag aagaagcgct
    22921 gaaagagaac acccaggaag ttcacgccat tgttcctgtg ccaatcgctt cttatctgct
    22981 gaatgaaaaa cgttctgcgg tgaatgcgat tgaaacgcgt caggacggtg ttcgctgcgt
    23041 gattgtgcca aacgatcaga tggaaacccc gcactaccac gtgttgcgcg tgcgcaaagg
    23101 ggaagaaacg ccgaccttaa gctacatgct gccgaagctg catgaagaag cgatggcgct
    23161 gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa caacctgcac tggcaacctt
    23221 tgccatgccg gatgtgccgc cagcgccaac cccagctgaa cctgccgcga ctgtcgtagc
    23281 cccagcaccg aaagctgcaa cggcaacacc agcagctcct gcacaacctg ggctgttaag
    23341 ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt gaagaagcca aaccgaccga
    23401 gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc gcaaacctcg
    23461 tcagagcaac cgccgtgacc gtaatgagcg ccgcgacacc cgtagtgaac gtactgaagg
    23521 cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag gcacagcagc agactgccga
    23581 gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa gcacgtacca ccgacgagca
    23641 gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat gataaacgtc aggcgcaaca
    23701 agaagcgaag gcgctgaatg ttgaagagca atctgttcag gaaaccgaac aggaagaacg
    23761 tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc aatcagaaag tgcgttacga
    23821 gcaaagcgta gccgaagaag cggtagtcgc accggtggtt gaagaaactg ccgctgccga
    23881 accaattgtt caggaagctc cagctccacg cacagaactg gtgaaagtcc cgctgccagt
    23941 cgtagcgcaa actgcaccag aacagcaaga agagaacaat gctgataacc gtgacaacgg
    24001 tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg cgcgtgagtg gtcagcgtcg
    24061 tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca atgccgttga ccgtagcgtg
    24121 cgcgtctccg gaactggcct ctggcaaagt ctggatccgc tatccaattg tacgtccgca
    24181 agatgtacaa gttgaagagc agcgcgaaca ggaagaagta caagtgcagc cgatggtgac
    24241 tggggtccct gtcgccgccg ctgtcgaacc tgttgttagc gcaccggttg ttgaagaagt
    24301 ggctgaagtt gtagaagccc ccgttcagat tgccgaaccg caaccggaag tggttgaaac
    24361 gacgcatcct gaagtgattg ccgccgcagt aactgaacag ccgcaggtga ttaccgagtc
    24421 tgatgttgcc gtagcccagg aagttgcaga acacgcagaa ccggtggttg aaccgcagga
    24481 agagacggca gatattgaag aagttgccga aactgctgag gttgtggttg ctgaacctga
    24541 agttgttgct caacctgccg cgccagtcgt cgctgaagtc gcagcagaag ttgaaacggt
    24601 agctgcggtt gaacctgagg tcaccgttga gcataaccac gctacggtgc cgatgacgcg
    24661 cgctccggca ccggaatatg ttccggatgc accgcgtcac agtgactggc agcgccctac
    24721 ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg gcaacacatc atgcctctgc
    24781 cgctcctgcg cgtccgcaac ctgttgagta ataattagct caagtaatca agccctggta
    24841 actgccaggg cttttttatt tcatctttga atcaacatat taaaattcat cgaatggcat
    24901 ccttgctaac caacaatgca aaataggcaa aaaaatgccc gccccggcga tcccagagca
    24961 ggcagacaaa acatacccag tttcatgata tgttttaaag cgaaattatt tgttgagctg
    25021 gaagagcgac aatccctgca tatcggtaaa tgctttatac gatgcctgca atgccgtttg
    25081 ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat cgctcatctg
    25141 ctgcgtttgc cctaaagcgc gatcgctacc taatgaatcc agcgactcca gttcgttcag
    25201 ctgcgtgcct aattccgcgc gaacggtcag cacattgttc agcgagtttt tcagtccgcg
    25261 gttggtttta tctaacgccg cagcggcggt ttctttatcc gcttcgctat ccgcgaccgg
    25321 cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt cagaagcgct
    25381 accgtctggt tccgctaccg cgttactggt aatactgtcg aaaattttgt cgcccgtatg
    25441 tcctatcacc atcgaacgcg aagcatcgac ctgttgctta atactttctg ttccgccgac
    25501 ataatcaccg ttagcctcac taaacggcgc agtctctgtt ttataaccgg caaaaatgta
    25561 gcgcccatta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa gcccctgaat
    25621 atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg cgtagacaat
    25681 tttttcctga gcattctgga tagcagtggt gacctggcta agtacactct cttccagtga
    25741 cactttttga gtggcgaaag tacgcgccag cgtgtactgg ctgttttgcg cctgtgcctg
    25801 ggagagaaca accgcttgtg atgcagcaat gggatcatct gaagggttaa cgactcgctt
    25861 acccgtcgac atctgttcgc cgtacttcat ccattctgcc tgagaattgg tgataccacg
    25921 catgttttgc tggtacatca tctgggtact gaaacgcatt ttatctcccc ttagcgaatg
    25981 ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa
    26041 tactgctgaa aacgttgcag atttccgtac tcctcatcga gattgacacc ggaaatcgac
    26101 tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggtgctact ggttttcaac
    26161 gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt aaaggatttc
    26221 gcaccgccca ccgttttact attgctttgc agatccagca gggcctgacc gttgcggtta
    26281 tcgctatcac ccgcatcttt ttcgctcgcc atcgctattt tcgcttcgtc ggtgattaat
    26341 acatccatgt tgacgatggc gtcacttact ggtttcagcg tgaagctgtc gttaacggca
    26401 ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt ggcatccggc
    26461 gtcaccgtaa aagtggtatt gctggcaagg cgggtgacct gccactgatt attatcgaac
    26521 gagattttgt aatctgtcgc cagtaccacg gaggcatcag ttaccgtggc accgatcgca
    26581 acgtcacctt tgtttttcgt gttttgcaga accgcgggct taccgatagt aaagaaatct
    26641 tcaccagcat cgccgttagc atcaaatccg gctttgtgtt gggtgttgaa agcctcggca
    26701 aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagctcctg agaacggaat
    26761 gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc aatattgcct
    26821 gccgtcccat caacataagc gacagtcgta cgagaagggt cagcgctgga aggaactgcc
    26881 gccagttgcc gcgccgtact tccctgaacc agtgagtaac cattggccac cgtgatgtta
    26941 taagtgccgc catcctgaac gctgacttct acaccaacaa tctggtttaa ttcgctcacc
    27001 agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac gcctgtcagg
    27061 cgcgagattt gatcgttcag gctggcaatt tgtttagcgt aattgttgat ctgatcaacg
    27121 ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gcagatattg atcggtggtt
    27181 ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc tgccgggtct
    27241 tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg tgttgccagc
    27301 gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata gcgggcggtc
    27361 aggccgctac tttgcgtctg cgcagcacgt aactggttgg taataaacgc atcatactca
    27421 cgctgcacac cagaaacgta gacgccattg ccaacccagc cgccagcgcc caacgtgcta
    27481 ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata gctggagata
    27541 ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat ggcgttattg
    27601 atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta tcggcagcga
    27661 cccgccggac ttgagttatt cagaacagat tatcaatgtt catgctgtag gttttgctca
    27721 ccttgtcgct tatcgatttc atctgctgga tcatgttggt gagtttacgg gcatagtgag
    27781 gatcggtagc ataccccgcg tcctgtaggg cctgcgcccc ctgctccgca ctcgcggctg
    27841 tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc aacgtaatcc gacaatgctt
    27901 ccagatacga gctgtagacc cgaaatttcg cttttacttt cttcgcttcg ccattttcat
    27961 attcagtcgt ggtgatttca gtaactggac ctttccagtt gccagaggct ttgacgccaa
    28021 acaggttata gctcggctcg ccgttttcgc ggcggatttg ccgttgcccc caaccagact
    28081 ccagcgccgc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg cttgccagtt
    28141 gggcgggcag cgagagttgc gcgaggaatg ctttactgtc acccggcagc gaatcatcgt
    28201 agttacgtgg cacggccttt tgcaccagct gcgaaagcgc ctgattttga taacgcacca
    28261 cagtttcgag cgggaatttc atcggtgctg ctggcgtgga ctcctctggc aatggttgtt
    28321 ctggcgtcat ctgtttaacc atcatctctg caagccccag acctttgccc gccgtcatct
    28381 gttgggcaat ctgctggtca tacatactgg tatacaggcg agtgtgctcg ctgctgaaca
    28441 ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg aacatccctt
    28501 ccacctgacg ggccaccgga cggatatttg ccgccggatc ttcgcccgct ttcgccttta
    28561 gttcgttgag tgattgcgca tcccaggccg cacttgccag tagtttgctg tcgctgatca
    28621 tcagatgatt tccagttttg cccgcagaca tcccgcactt tgcattgatt gcagtatgga
    28681 catcagatcc atcggcgtag cgcccagcgc attgagcgcg cgcaccacgt tattgaggct
    28741 ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg cgtaaatcga tctgcgtttg
    28801 tggagtaacc acagtctgtc caccaccaaa cggtgtatct ggctggctga cattggcctg
    28861 acgattgact gttactgaga gatttccctg cgctaccgcg cagctgtcga gggtcacttc
    28921 gcgattcatc accaccgaac cggtgcgcga gttaatcact actttagcgt cctgcggggt
    28981 gacattaacc tgcatattct ggatatctgc aaggaaacgg acctgggaac tgttgccact
    29041 tggtacgcgc acctgaatag tccgcgcatc taacgcggtg gcgctgccat atccacgcac
    29101 gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa tcttcgtcgt taagttgcaa
    29161 attaagggta ttcccgacgc caaactggct gggcaattca cgttcaataa ccgcaccatt
    29221 ggtgatccgt ccaccgttca gttggttcac ctgaacactg ctaccgccag cggaggctcc
    29281 tgcgccgcca accagaatat tgccctgcgc cagcgcatac acctgactgt caacgccctt
    29341 aagcggcgtc atcaacaacg tacctccacg caagcttttg gcatttccca tggaagaaac
    29401 caccacatcg atggtttgcc cctgacgtcc aaacggagga agtgactctg tcaccattac
    29461 cgcagcgacg ttttttagct gcatattggt gcccgtcgga acggtaattc ccagctgtga
    29521 gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg gttgtctggt caccggtgcc
    29581 atccagcccc accaccagac catagccaat cagtgagttt tgccttaccc cctgaacact
    29641 ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta accagtagaa gaattaatgc
    29701 agagagaaat ttaatcacca cagcctcact tacattggcg acaggttaag gaagaaacgc
    29761 tgcaaccagc ccatattttg cgcttcgtta atgtagccat tgcctacgta ttcaatgcgc
    29821 gcatccgcca cctgagtaga cggtacggta ttgctgccgc tgatagtgcg tggattaacc
    29881 acgccagaga agcgaataaa ttcggtaccc tgattaatgg caatctgttt ttcacccacc
    29941 acatgcaggt tgccgttgac cagtacctgg tcaaccgtca ccgtcaacgt gccgctaaag
    30001 gtattgctgg cattggcccc gccctttccg ttgaacgtgt taccaccgga ggcttcgaca
    30061 tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc aaagccaaaa
    30121 ttagttttac cgtcacggct ggcattcgca gaggagcttt tgctggcgct gacgttctcc
    30181 tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc aaacagcggt
    30241 tgatagccat agttaatcgg ctgagcagac tggaaaatag aaccgttggc gacgggcgtc
    30301 ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca gcggcgtgga gggtatccag
    30361 gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg cataagtatg cgcagcgttt
    30421 ttttgcatgg ctatcatctt caaaaatcag taaaccggtg aacctgccac cggttaagcc
    30481 ttagagttgc gtcagttttt gcagcatctg atcggtggtg gacaccgctt tactgttgat
    30541 ttcgtaagcg cgttgcacct gaatcatatt gaccagttct tccgccacgt tgacgttaga
    30601 cgtttcaaca tacccttgat acagcagtcc cgcgccgttc aggcccggcg tgctttcgtt
    30661 cggtacacca gaggattgcg tttcggtgta gaggttttcg ccaatgctct ccagcccggt
    30721 gtcattcata aaggtggtga gattgagctg cccaacctga accggagctg cctggccttg
    30781 ttgggttacg ctgaccacgc catcacgacc gatggtgata cttaacgcat tcgccggaat
    30841 ggtgatcgct ggctgcacct gaaaaccacc agctgcccgt tctgatccac ctggaaagag
    30901 ccgtcacggg tataggctga tgaaccatct ggcaacatca cctggaaaaa gccctgccct
    30961 ttaatcgcga catctttgct gttgttggtc tgcgacaggt ttccctggct gtgtaagcgt
    31021 tcagtggcga ccgggcgtac ccccgtgccg atttgtaatc cggagggtaa ggtggtttgt
    31081 tcggaagact gtgcccccgg ctggcgaatg gtttgataaa gcagatcttc aaacaccgcg
    31141 cgctgacgct taaaaccgtt agtactgacg tttgccaggt tgttggcaat gacgtccata
    31201 ttggtttgct gggcgtcaag gcccgttttg gcgatccata atgaactgat cataaaatgt
    31261 cctgtagtgg attagtgaca aagtgcatat gtcttcttgc cggatgcggc gtaaacgcct
    31321 tatccggcct acaagtgctc gcaaaatcga taaattgcga ttgcccccat agacccgata
    31381 aacttgcgca tcgggcaact tatacttgtc atgtatcctt tcaattaact catcgacagc
    31441 agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca
    31501 aaacgccgcg cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct
    31561 tccagaaccc ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg ccctcgcgtg
    31621 gcctgggttt ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc
    31681 gtggctttca ccagtttcag acgccctact ggcgcaactg tatttgccgg atcgcccgga
    31741 ttgagcgccg agattgtgcc atcggcagca atagtgattt ccgctccttc cggcacagca
    31801 attggccccg cctcgcctat cacctgatgc ccctgaattg tcagttgccc ggtgggatca
    31861 acctgaatgc tgccattacg cgtataccct tcgctgccgt cagcggtctg cacggccagc
    31921 cagccatcct gctgcaacgc gacgtccagc gggcgcgagg tgtaatccat tttgccgggc
    31981 gtcatatctg cgcccggcgt tgacgccgtg accaacgtgc gcgtgggcag agaaagccct
    32041 tccactggca ccgcgcgtaa agcattcaac tgcgcgcgaa aaccgggcgt tgaggcattg
    32101 gccagattac tggcggttac cgcctgttga ttcagtgtct ggctggctgc tcccatcgcg
    32161 gtataaattg cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt taaccagcgt
    32221 gttgaggatc tggtcctgag ttttgatggt ctgggcgtta gactgatagt tacgctgggc
    32281 aacgatcata ttgaccagtt ctttactgag atcgacattg gacgcttcca acgcaccgtt
    32341 ggtcagggtg ccaaagttgc ccgtcccggc tgtccccaac agcgccacgc cagaagattg
    32401 cgtcgcagac cagacgttgt cgccttcgga tgccagacct tcgttgttgg caaagttcgc
    32461 cagtacaatc tgccccagca gttgtgtttg ttcgttggaa tagttgccga caaccgtacc
    32521 gtcatcattg atttgataac tcaccagatc gcccggtttg tagccattct gggtggttgc
    32581 cacaatattg ttagcgccgg tattttgctg catggagttg aggaagctca ggctaaacgt
    32641 ggcgggatct gcgccgttaa ttgcgccagt ggtaatattc tctgttggat ttgaggtcag
    32701 aacgccattg gcattaaaca ccagcttcat tgcaggctcg gctgtacctg ttggatcact
    32761 gctatcctgg gtgtagacct gccagttatt atccccggtc ttcacaaagt agacgctcat
    32821 gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc
    32881 cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga tcactggaat tcaggttgat
    32941 ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt
    33001 ggtcggattc gccccttgct gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag
    33061 ctgtaaacct tgcatattca ccaggttacg gttttcatcc agcttaaatt gtccgttacg
    33121 gctgtagaac accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc
    33181 aacgtccaga cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc
    33241 ggcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt
    33301 gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt
    33361 ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa
    33421 tgtgaaggct taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag
    33481 atccagcgta ttaccgctgt tgccgcggag cacaccctgc accagagcaa actgcagcgg
    33541 ctgggcaacc agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt
    33601 cggcgcagtg ctgccatcag tcaacgtacc gtcccaggtg aaactgtgga ctccggcggt
    33661 cagttcacca atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt
    33721 aactttgtct gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc
    33781 ttcttcactg ccggttcccg caagaacagt ggtgccgggg atcatcacgc cgtgaccgat
    33841 caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt
    33901 ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc
    33961 gttgttttcc attggattgg tcgggtcctg gtttttcagc tgcgccacca acaaagtcag
    34021 aaaactgctt tgtaagtctg cggcgttgct gcccgtgagc gaactactgc tggtggtact
    34081 gacgccggta tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat
    34141 tgaccgagcg taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg
    34201 gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttc
    34261 ggcattttta cgtagccctt tgcatccgcc agcggattac ccggttcata aaccagtttg
    34321 tccggggcct gactttctat aacatcggca acctttacgc cgcctgtcgc agcacctggt
    34381 gcagcgttaa cctggaatac cacctgtttt gcccgatatg gctgtccatc gggaccggtc
    34441 acgctatcag cattcgccag attactggcc gccacgttca agcgctggga ctgggcagtt
    34501 aacgccgacc cggcgatatc aaaaatattc agcagtgcca tccgttaatt tccgctctgt
    34561 aaaacgttca tcatgccttt gatttgcccg ctcaacgcgc taaggctcat ctggtattgc
    34621 aggctgttat cggcaaactg ggtgcgttcg cgatccatat cgacggtatt accgtcaagc
    34681 gaaggctggt ccggaatacg gtattgcagt tctgcggtag gaggcgtcag cgcctgcgcc
    34741 ggaatgtgtt gcgttgaggt catcgtcagt gcaaccacac tggttgcatc ccgtccacgt
    34801 tgcatgactt ttttaagttc actggcaaaa tcgatatcgc gcgcctgata accaggggta
    34861 tcggcattgg cgatgtttgc tgccagcact tcctgacgct gggcgcgcag attgagcgcc
    34921 tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca
    34981 aaattctgcc atcagcttaa atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa
    35041 tgggtcgcta tttatgccgt tgatgaccat tgcggacagg tacaattcac gttgtagaaa
    35101 tggctggggg cgaaaatgct ggcaataaaa cgtagcgtgg cgatcatctc gatactattc
    35161 actccgttaa gtgcggcgag taatctcaca tcgcaattgc aaaccttttt tagcgcccag
    35221 ctcgcggggg taagtgatga ggttcgtgtt tctattcgta cagcgcccaa tctactaccg
    35281 ccgtgcgagc agccattgct ttcgatgagc aataattccc gcctgtgggg caatgtgaat
    35341 gtgttggcac gctgcggtaa cgacaaacga tatttacagg ttaatgtaca ggccacagga
    35401 aattatgtgg ttgccgcgat gcccattgtg cggggaggaa agctggaagc tggcaatgtc
    35461 aaactaaaac gcggacggca ggataccctg ccaccgcgta cggtgctgga tatcaatcaa
    35521 cttgttgatg ccgttagcct gcgcgatcta tcacccgatc aacctatcca gttaacccag
    35581 tttcgccagg catggcgggt aaaagcggga caacgcgtca acgtgatcgc cagcggtgac
    35641 gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg cagccgtcac acagaatgcg
    35701 cgggtgcgca tggtatcggg actggtggtc agcggcgttg ttgatgcaga tgggaatatt
    35761 cttataaacc agtaagctgt taaagattac ccgtcccttg ccgataaata agcaacacat
    35821 gataaaagcg ccctcaatga ggaataaacc atgagtattg atcgcacttc gcctctgaag
    35881 cccgtaagca ccgttcaacc acgcgaaacc actgacgcgt cggtaacgaa cacccgggcg
    35941 gcaaaaacaa cagcctccac cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg
    36001 atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa actggcgatt
    36061 cgtaacggtg aactaaaaat ggacaccggc aaaattgccg atgcgctgct caacgaagcg
    36121 cagcaagact tgcagagtaa ctgatcgtat gacacgtctt gcagagatcc tcgaccagat
    36181 gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa gaacagcaac atctctctat
    36241 ggggcagatc aacggcagcc agttgcaatg gattacagaa caaaaaagct cactgctggc
    36301 gacgctggat tacctcgaac agttacgcag gaaagaaccc aatacagcaa atagcgttga
    36361 tattagtcaa cgctggcagg aaattactgt gaaaacgcag caactacgcc aaatgaatca
    36421 acataacggc tggttactgg aaggacagat tgagcgcaat caacaggcgc tggaaatgtt
    36481 gaaaccgcat caggaaccga cgctatatgg ggcgaacggt cagacctcaa caacccatcg
    36541 cggcggtaaa aagatttcga tctgaatatt atctccggcc tgcactgcag gccggaatgc
    36601 attgttacac cgtccggcgg gcaaattctt taactttgaa gcccagtacc gccagtgcag
    36661 cgaagtacgc ggcaatcccc gccagcacga ccgccattaa acgcagtaag cgccagggca
    36721 tggtacccaa tgaccactcc ggcatgatat gtaacatacc taaaagcacg ccagacatca
    36781 ccagtaccgc caccaccaga cgcaacagaa acgccatcca gccgggttgc ggagtaaaga
    36841 ttttctgctt acgcaactgc cagtaaagca gcgaagcatt cagacacgcc gccagaccaa
    36901 tagaaagtga cagcccagca tgtttcaacg gaccaataaa cgccaggttc atcaattgcg
    36961 ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg cgggaataaa
    37021 agccaggagc caacactttc actacaatca ggccaatcaa acccaccgag taggcaatta
    37081 acgcccgctg ggtcatcagc gcatcaaacg cggtaaattt accgtactgg aacagcgaaa
    37141 cggttaacgg acccgaaaga atgcccaacg caaccgcact cggcagcgcc aacaggaaac
    37201 aaagacgcaa cccccagtcc atcaaacggt tgtattcatc atgattgcca ctggcaaaac
    37261 ttttcgacag cgacggcagc aaaatggtgc caagcgccac gcccagcaca ccggagggaa
    37321 actccattaa gcggtcggcg taatacatcc aagacaccga accggaagca agaaacgagg
    37381 caaaaatggt gttgatgatt aaggagatct ggctcacaga gacgccaagg atcgccggtc
    37441 ccatctgttt caccacgcgc attgctccgg catcgtggaa gttaatgcgc ggcaggacca
    37501 gcatgccgat cttcttcagg tgcggtagct gatacaccag ctgcaggatg ccgccgaccg
    37561 taacagccca cgccagcgcc agcaccggtg ggttaaagta cggtgcggca aacagcgcga
    37621 aaccaatcat gctgatgtta agcagtgttg gcgcaaacgc cggaatcgag aagcggttcc
    37681 acgtattcag aatcgctccc accagcgacg ccagggagat cagcaagata tagggaaagg
    37741 taatctttag tagctggctg gtcagggcaa atttgtcagc tgtgtcagcg aagcctggcg
    37801 cggtcaccat gatcacccac ggtgcggcga gcatgccagc gaccgtcaca accgccagcg
    37861 caagtgtcag caggccagaa acataagaga caaagacccg cgtggcgtct tcaccctgct
    37921 tacttttata ttccgccaga atcggtacaa atgcctggga aaatgcccct tcggcaaaga
    37981 tacggcgtaa caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg
    38041 cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc aagcacacgc gaaaacatgg
    38101 tcatcgagct gacggcggcc agcgatttta ataaattcat cggtgttcta atccagaccc
    38161 acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga atagtctacc tggattatgg
    38221 tgaattgcta ccgccagatg ttacagggtt tattcactca tcgcatcgcg ccagatcttg
    38281 tcaacgatac gttgcgccag cactgcctgt tcgccggcgg tttgcagaac tgtctgattt
    38341 tgcacacatt caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc aagagtgctc
    38401 tgccaaccag gaatcggttt atgcactacg ccctgcccgc gctcctcacg ccattcgcgc
    38461 atatccgtaa tgtcgatgag cgcatcgtca gtcacggcct gcacggtttc acgctgactt
    38521 ccggcacggc gatgcataca ggtggtgatc tgcaaaggat cggccgaaaa atggtgctcg
    38581 gcaaacagca tttcgccagc gtcgttagtc agtagcgtac cgccatccag agaggctttg
    38641 ccgcccgaca accacagcgc ggtatccacc acatgcagat aatcatccag caacgtgaaa
    38701 taaagatcgt gtggcccgac actattgcta cgatgtttat ccattcttag cgaagctgcg
    38761 gtagcgagtt gcgtttttaa ctcaccgtag agtggtgcga aacgacggtt aaaaccgacc
    38821 atcaacgtca gttttttccg cgccgccagt tccaccagcc gttcagcatc gcgcagattt
    38881 tctgccagcg gtttatcgac acagacatgt acacccgcat tgagtaacgt actgaccacg
    38941 tcaaagtggc tggcggtgct ggaatgcaca aaaaccgcat cgcaactggc ggcaaggctg
    39001 gataacgaat cggcataagg aatgcgccag ctttcacaaa ttggcagggc tttcgcgcgc
    39061 gtaggcgacc aggctccttg taacatccag tcagacgctg ccgccagcac cggtaaccac
    39121 gctttttgcg caatgccgcc taatcccaca acgccgatac gtaatttttt cactttaatc
    39181 tcccagatgg gccagcaacg aatcaagacg ctgtttcagt tctgccactt cgatttccag
    39241 ggcttcgacg cgggcctgta aatcaccatc aaccgcgttc gacatatccg ttaccgccgg
    39301 ctgatcctct acctcaccac tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc
    39361 gcgggccaga cgcaccacaa aaggaccatc ttcgcgattt gccagttgtt ccagcgtcga
    39421 ctccacttcc gccatatcgc tgaattcata cattcgcgcg gcgcggctgc gcagttcacc
    39481 cggcgtctgg gcaccacgca ataacaacgt ggtgattaac gccacttccg ctgcgctcag
    39541 tttcagatcg ccaaattctg aattacaaaa acgttgttca tatttggtga cccgattacc
    39601 aaagccactc accgtgcgca gataatggcg tttgaccaga ttatccagtt gctcctgcac
    39661 ttcagattca ctcagattca tgaccggttc acggttcgtt ttctgattac aggccgtgac
    39721 tacaccattg actgagagcg ggtattgctc cggcgtggtc acctgctttt ccagcaagca
    39781 accaatcacg cgagcttcaa gggcagtaag ttgatatttc atgagattct ccttagcggc
    39841 cgggcgtcca gtctggggta gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa
    39901 tcaacagata gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac
    39961 cgctgcgttt attgtgcggc atataattag ccataatgcg atgaatatgt tgggtgcgct
    40021 gcatataacg aatggctgcg gtcagggctt caaacatgag tcctttgccc tgccattttt
    40081 gcccaatcga ataaccgaga tagcaggcat gaaaagagcc acgaacaaca ttggaaaaat
    40141 tggcaacgcc aataatctct ttttcgtccg ggtcgaataa gccaaagtag aaagctgaac
    40201 cttgtttatg aaattcgtta atcatcccca gcctggcctg ccagcctgat ggataacagt
    40261 ggctttcgtc gcgcactggc tcccagggct tgaggaaatg gcgattctct gcgtaataat
    40321 ccgcaagacg ccaggcatca cgatcatgca ccagacgcac gaccagtcgg tctgtggtta
    40381 agcgcacttt tggcacgtta ctgcgatagc caaacattct atacctactc cttcccgtaa
    40441 cgtctttaat acgctgtaag tattactata cctgcgcaac cagaccctgt gaaaagtcca
    40501 aaatgccatt ttctgcatta ttcacatatt tgataaaaac tctctatcaa agcgacgttt
    40561 tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct tagcggcaat gcagaataga
    40621 aagtgctcat cttttatttc ccgggagggg aaatgtcccg cgtatcgcag gcgaggaacc
    40681 tgggtaaata tttcctgctc atcgataata tgctggtcgt gctggggttc tttgttgtct
    40741 tcccgctgat ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc atggtcggta
    40801 ttgctctcgg tctacgccag tttattcagc aaggtctggg tattttcggc ggcgcaattg
    40861 ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat gctgatgcgc gccgccggat
    40921 tcgccacaat gggtatcgcc cacgaaccgt ggctattgtg gttttcatgc ctgctctcgg
    40981 gactcggcgg cacgttgttt gatccgccgc gttcggcgct ggtggtgaaa ctaatccgtc
    41041 cacaacaacg ttgtcgtttt ttctcgctgt tgatgatgca ggacagtgcc ggtgcggtca
    41101 ttggcgcatt gttggggagc tggctgttgc aatacgactt tcgcctggtc tgcgccacag
    41161 gggccgtttt gtttgtgctg tgtgcggcgt tcaatgcgtg gttgttaccg gcatggaaac
    41221 tctccaccgt acgcacgccc gttcgcgaag gcatgacccg cgtgatgcgt gacaagcgtt
    41281 ttgtcaccta tgtcctgacg ctggcgggtt actacatgct ggctgtacaa gtgatgctga
    41341 tgctgccaat tatggtcaac gacgtggctg gcgcgccctc tgccgttaaa tggatgtatg
    41401 ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc
    41461 attttcgtct ggaacaccgg ttgatggctg ggctgttgat aatgtcatta agcatgatgc
    41521 cggtgggcat ggtcagcggt ctgcaacaac ttttcaccct gatttgtctg ttttatatcg
    41581 ggtcgatcat tgccgagcct gcgcgtgaaa ccttaagtgc tttgctggcg gacgcaagag
    41641 ctcgcggcag ctatatgggg tttagccgtc tgggtctggc gattggcggc gctattggtt
    41701 atatcggtgg tggctggctg tttgacctgg gcaaatcggc gcaccagcca gagcttccgt
    41761 ggatgatgct gggaattatt ggcatcttca ctttccttgc gctgggttgg cagtttagtc
    41821 agaaacgcgc aacgcgtcgt ttgctggaac gcgacgcctg atttgatact cccccctgac
    41881 tcctccatac tgaaagtgaa ctgacagcca aatggaggag tcacgtgaag ctatacattt
    41941 acgatcactg cccttactgc ctcaaagccc gcatgatttt cggcctgaaa aatatccccg
    42001 tcgaattaca tgttctgctc aacgacgacg cagaaacacc cacccggatg gtcggtcaaa
    42061 aacaggttcc cattctgcaa aaagatgaca gccgctatat gccagaaagc atggatatcg
    42121 ttcactatgt cgataaactc gacggcaaac cgttactgac cggcaaacgt tcccctgcca
    42181 ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa actgctgttg ccgcgttttg
    42241 ccaaatcggc atttgatgag ttttctactc ccgccgcgcg caaatatttc gtcgacaaga
    42301 aagaggccag cgcgggtaat tttgccgacc tgctggccca ctctgacggt ctgattaaga
    42361 atatcagcga tgatttgcgt gcgctggaca aactgatcgt caaaccgaac gccgtgaatg
    42421 gcgaactttc ggaagatgat attcagctat ttccgctact gcgtaatctg acgctggtag
    42481 ccggaatcaa ctggccaagc cgcgttgctg attaccgcga taatatggcg aaacagacac
    42541 aaatcaattt gttatcatca atggcgattt aatcctgacc cgccgcggca gcctgtattc
    42601 gcgggttgcc gccgttttca gttttttctg ctggcgagtt tatcgctggt gacacatgct
    42661 gcgttgtgtc aggatattga cgcagatttc atgtcgagga ctaccatgaa caagttttta
    42721 tttgccgctg cattgatcgt cagtggcctg ctcgttggct gtaatcaact cacccaatac
    42781 accatcaccg aacaagaaat taaccagtcg cttgcgaaac ataataattt ctcaaaagat
    42841 atcggtttac ccggcgtggc tgacgcccat attgttctga caaacttgat cagccaaatt
    42901 ggtcgcgaag agccgaataa ggttacccta accggagacg ccaatctgga catgaactcc
    42961 ctgttcggta gtcagaaagc gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat
    43021 aaagaaaaag gtgcgatctt cctgaaagag atggaagtgg tcgatgcgac ggtacaaccg
    43081 gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt tgaaccaggc attacgcaat
    43141 tactttaacc agcaacctgc ttacgtcctg cgcgaagatg gcagtcaggg cgaagcaatg
    43201 gcgaaaaaac tggcgaaagg cattgaagtg aagccaggcg aaattgtcat tccgtttacg
    43261 gattaatgac gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc
    43321 ttatcctttg tgtccggcaa aaacattcct tcagccggag catagagatt aatgactgca
    43381 ccatcccagg tattaaagat ccgccgccca gacgactggc accttcacct ccgcgatggc
    43441 gacatgttaa aaactgtcgt gccgtatacc agcgaaattt atggacgggc tatcgtaatg
    43501 cccaatctgg ctccgcccgt gaccacagtt gaggctgccg tggcgtatcg ccaacgtatt
    43561 cttgacgccg tacctgccgg gcacgatttc accccattga tgacctgtta tttaacagat
    43621 tcgctggatc ctaatgagct ggagcgcgga tttaatgaag gcgtgttcac cgctgcaaaa
    43681 ctttacccgg caaacgcaac cactaactcc agccacggcg tgacgtcaat tgacgcaatc
    43741 atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgc tactggtgca tggtgaagtg
    43801 acacatgcag atatcgacat ttttgatcgt gaagcgcgct ttatagaaag cgtgatggaa
    43861 cctctgcgcc agcgcctgac tgcgctgaaa gtcgtttttg agcacatcac caccaaagat
    43921 gctgccgact atgtccgtga cggaaatgaa cggctggctg ccaccatcac tccgcagcat
    43981 ctgatgttta accgcaacca tatgctggtt ggtggcgtgc gtccgcacct gtattgttta
    44041 cccatcctca aacgcaatat tcaccaacag gcattgcgtg aactggtcgc cagcggtttt
    44101 aatcgagtat tcctcggtac ggattctgcg ccacatgcac gtcatcgcaa agagagcagt
    44161 tgcggctgcg cgggctgctt caacgcccca accgcgctgg gcagttacgc taccgtcttt
    44221 gaagagatga atgctctgca gcactttgaa gcattctgtt ctgtaaacgg cccgcagttc
    44281 tatgggttgc cggtcaacga cacattcatc gaactggtac gtgaagagca acaggttgct
    44341 gaaagcatcg cactgactga tgacaccctg gtgccattcc tcgccgggga aacggtacgc
    44401 tggtccgtta aacaataaaa atcgtagctt cctgttgtca ttaggttatt taacctgtat
    44461 aaataaccag tatattcaac agggggctat tatgcgaatt gaagtcacca tagcgaaaac
    44521 ttctccattg ccagctgggg ctattgacgc cctggctggc gaactttccc gccgtattca
    44581 gtatgcgttt cctgataatg aaggccacgt atcggtacgt tatgccgcag cgaataattt
    44641 atcggttatt ggcgcaacaa aagaagataa acagcgcatt agcgaaattc tccaggaaac
    44701 gtgggaaagc gccg
//
LOCUS       DAZOYL010000003        44712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZOYL010000003 DAZOYL010000000
VERSION     DAZOYL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44712
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.003"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(83..1489)
                     /gene="gndA"
                     /locus_tag="ACHUOY_000100"
     CDS             complement(83..1489)
                     /gene="gndA"
                     /locus_tag="ACHUOY_000100"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR0681002.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(1653..3023)
                     /gene="cpsG"
                     /locus_tag="ACHUOY_000101"
                     /gene_synonym="manB"
     CDS             complement(1653..3023)
                     /gene="cpsG"
                     /locus_tag="ACHUOY_000101"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR0681003.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(3128..4564)
                     /gene="cpsB"
                     /locus_tag="ACHUOY_000102"
     CDS             complement(3128..4564)
                     /gene="cpsB"
                     /locus_tag="ACHUOY_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR0681004.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(4567..5790)
                     /gene="wcaI"
                     /locus_tag="ACHUOY_000103"
     CDS             complement(4567..5790)
                     /gene="wcaI"
                     /locus_tag="ACHUOY_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR0681005.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(5787..6266)
                     /gene="gmm"
                     /locus_tag="ACHUOY_000104"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(5787..6266)
                     /gene="gmm"
                     /locus_tag="ACHUOY_000104"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR0681006.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(6269..7234)
                     /gene="fcl"
                     /locus_tag="ACHUOY_000105"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(6269..7234)
                     /gene="fcl"
                     /locus_tag="ACHUOY_000105"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR0681007.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(7237..8358)
                     /gene="gmd"
                     /locus_tag="ACHUOY_000106"
     CDS             complement(7237..8358)
                     /gene="gmd"
                     /locus_tag="ACHUOY_000106"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR0681008.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(8384..8932)
                     /gene="wcaF"
                     /locus_tag="ACHUOY_000107"
     CDS             complement(8384..8932)
                     /gene="wcaF"
                     /locus_tag="ACHUOY_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR0681009.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVKLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(8948..9694)
                     /gene="wcaE"
                     /locus_tag="ACHUOY_000108"
     CDS             complement(8948..9694)
                     /gene="wcaE"
                     /locus_tag="ACHUOY_000108"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR0681010.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(9705..10922)
                     /gene="wcaD"
                     /locus_tag="ACHUOY_000109"
     CDS             complement(9705..10922)
                     /gene="wcaD"
                     /locus_tag="ACHUOY_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR0681011.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(10897..12114)
                     /gene="wcaC"
                     /locus_tag="ACHUOY_000110"
     CDS             complement(10897..12114)
                     /gene="wcaC"
                     /locus_tag="ACHUOY_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR0681012.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(12111..12599)
                     /gene="wcaB"
                     /locus_tag="ACHUOY_000111"
     CDS             complement(12111..12599)
                     /gene="wcaB"
                     /locus_tag="ACHUOY_000111"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR0681013.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(12602..13441)
                     /gene="wcaA"
                     /locus_tag="ACHUOY_000112"
     CDS             complement(12602..13441)
                     /gene="wcaA"
                     /locus_tag="ACHUOY_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR0681014.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(13534..15696)
                     /gene="wzc"
                     /locus_tag="ACHUOY_000113"
     CDS             complement(13534..15696)
                     /gene="wzc"
                     /locus_tag="ACHUOY_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR0681015.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(15699..16142)
                     /gene="wzb"
                     /locus_tag="ACHUOY_000114"
     CDS             complement(15699..16142)
                     /gene="wzb"
                     /locus_tag="ACHUOY_000114"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR0681016.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(16148..17287)
                     /gene="wza"
                     /locus_tag="ACHUOY_000115"
     CDS             complement(16148..17287)
                     /gene="wza"
                     /locus_tag="ACHUOY_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR0681017.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            17596..17745
                     /locus_tag="ACHUOY_000116"
     CDS             17596..17745
                     /locus_tag="ACHUOY_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681018.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            17946..19529
                     /locus_tag="ACHUOY_000117"
     CDS             17946..19529
                     /locus_tag="ACHUOY_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0681019.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(19803..21656)
                     /gene="asmA"
                     /locus_tag="ACHUOY_000118"
     CDS             complement(19803..21656)
                     /gene="asmA"
                     /locus_tag="ACHUOY_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR0681020.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(21678..22259)
                     /gene="dcd"
                     /locus_tag="ACHUOY_000119"
     CDS             complement(21678..22259)
                     /gene="dcd"
                     /locus_tag="ACHUOY_000119"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR0681021.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(22351..22992)
                     /gene="udk"
                     /locus_tag="ACHUOY_000120"
     CDS             complement(22351..22992)
                     /gene="udk"
                     /locus_tag="ACHUOY_000120"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR0681022.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            23310..26627
                     /locus_tag="ACHUOY_000121"
     CDS             23310..26627
                     /locus_tag="ACHUOY_000121"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0681023.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(26737..27585)
                     /gene="alkA"
                     /locus_tag="ACHUOY_000122"
     CDS             complement(26737..27585)
                     /gene="alkA"
                     /locus_tag="ACHUOY_000122"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR0681024.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            27719..29071
                     /gene="yegD"
                     /locus_tag="ACHUOY_000123"
     CDS             27719..29071
                     /gene="yegD"
                     /locus_tag="ACHUOY_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0681025.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(29084..31024)
                     /gene="yegI"
                     /locus_tag="ACHUOY_000124"
     CDS             complement(29084..31024)
                     /gene="yegI"
                     /locus_tag="ACHUOY_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR0681026.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(31021..31782)
                     /gene="pphC"
                     /locus_tag="ACHUOY_000125"
     CDS             complement(31021..31782)
                     /gene="pphC"
                     /locus_tag="ACHUOY_000125"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR0681027.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(31779..32438)
                     /locus_tag="ACHUOY_000126"
     CDS             complement(31779..32438)
                     /locus_tag="ACHUOY_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0681028.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(32659..32718)
                     /gene="ibsA"
                     /locus_tag="ACHUOY_000127"
     CDS             complement(32659..32718)
                     /gene="ibsA"
                     /locus_tag="ACHUOY_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR0681029.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(32991..33047)
                     /locus_tag="ACHUOY_000128"
     CDS             complement(32991..33047)
                     /locus_tag="ACHUOY_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0681030.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(33318..33374)
                     /locus_tag="ACHUOY_000129"
     CDS             complement(33318..33374)
                     /locus_tag="ACHUOY_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0681031.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            33653..34894
                     /gene="mdtA"
                     /locus_tag="ACHUOY_000130"
     CDS             33653..34894
                     /gene="mdtA"
                     /locus_tag="ACHUOY_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR0681032.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            34894..38016
                     /gene="mdtB"
                     /locus_tag="ACHUOY_000131"
     CDS             34894..38016
                     /gene="mdtB"
                     /locus_tag="ACHUOY_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR0681033.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            38017..41094
                     /gene="mdtC"
                     /locus_tag="ACHUOY_000132"
     CDS             38017..41094
                     /gene="mdtC"
                     /locus_tag="ACHUOY_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR0681034.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            41095..42510
                     /locus_tag="ACHUOY_000133"
     CDS             41095..42510
                     /locus_tag="ACHUOY_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0681035.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            42507..43910
                     /gene="baeS"
                     /locus_tag="ACHUOY_000134"
     CDS             42507..43910
                     /gene="baeS"
                     /locus_tag="ACHUOY_000134"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR0681036.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            43907..44629
                     /gene="baeR"
                     /locus_tag="ACHUOY_000135"
     CDS             43907..44629
                     /gene="baeR"
                     /locus_tag="ACHUOY_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR0681037.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        10228 a        12017 c        11631 g        10836 t
ORIGIN      
        1 atttagtcta taagataaat ggcaaaaaaa aagcccggtg caatatacgc cgggcctcaa
       61 ttttactgtt ggttaaatca gattaatcca gccattcagt atggaacaca ccttctttat
      121 caatgcgctt ataagtatgt gcaccgaaat agtcacgctg tgcctggatc aggttcgcag
      181 gcagaacagc ggcacggtag ctatcgtaat aggcaaccgc agcggcgaag gtcggaaccg
      241 ggataccgtt ctgtactgca taagcaacga catcacgcag cgcctgctgg tagtcatcgg
      301 caatttgctt gaagtacgga gccagcagca ggttagcgat ctgcggattt tcggcatatg
      361 catcggtgat tttctgcagg aactgcgcac ggatgatgca gccagcacgg aaaatcttcg
      421 cgatttcgcc gtagttcaga tcccagttgt attcttcaga cgcagcacgc agctgagaga
      481 agccctgagc gtaagaaacg attttgccca gatacagcgc acggcgaact ttttcgatga
      541 actcagcctt gtcgcctgct gactgagctt gcggaccaga gagaacttta gatgcggcaa
      601 cgcgctgatc tttcagagaa gagatataac gtgcaaacac agactcggta atcagcgaca
      661 gcggttctcc gagatccagc gcgctctggc tggtccattt accggtacct ttgttagccg
      721 cttcatccag gatcacatca accaggtagt taccgtcttc atctttttta gtgaagatat
      781 ctttggtgat gtcgatcagg tagctgctca gttcaccgtt attccactcg gtaaaggtct
      841 gtgccagttc ttcgttagag agattcaggc cgcctttcag gagagaatag gcttcagcaa
      901 tcagttgcat atcaccgtat tcaataccgt tgtgaaccat cttcacataa tggcctgcgc
      961 catcagcacc gatataggta acgcacggtt cgccatcttc agcaacggcg gcgattttgg
     1021 tcaggatcgg cgcaaccagt tcataggctt ctttctggcc accaggcatg atggacggcc
     1081 ctttcagcgc cccctcttct ccgccggaaa caccggtacc gatgaagtta aagccttcgg
     1141 cagaaagctc acggttacga cgaatggtgt cctggaagaa ggtgttacca ccatcgatga
     1201 tgatgtcgcc tttatcgaga tatggtttga gcgaatcaat agcagcatcc gtgcctgcac
     1261 ctgctttcac cattaacagg atgcgacgag gcgtttccag agattcaaca aactctttca
     1321 ccgtatagta aggaaccagt ttcttgcctg gattttcggc aatcacttct tccgtctttt
     1381 cacgggaacg gttgaaaata gagacggtat aaccacggct ttcgatgttg agcgcaaggt
     1441 tgcgccccat cactgccata ccgactacgc cgatctgttg ctttgacatt gtttactcct
     1501 gtcagggggt ggtcaccgcc gagtgtgcgg cttaaatgtg gtatagatgt taactcactc
     1561 atagagatgg tgaatagcat tacctttgct ataccacact gaatatctca ccttttccgt
     1621 cttttaaaac ttatttatat gaaattactg aattacttgt tcagtaactc aagaataagt
     1681 tttgtctttt cttccatcag cggcacatca ccgcgagatt ccacattcaa ccgcaccacc
     1741 ggttcggtgt tggaggtgcg caggttaaag cgccagtcgg caaaggtcat gctgatgcca
     1801 tcggtgcgat ccaccgccag cgcctcgcgg ctaaaatgct gttccacgcg gttaatcgcc
     1861 tcaaagggtt gcgccagttt gctgttgatc tcaccgcttg ccggaaacgc tgccatccgg
     1921 tcgcgcacca gttcgcccag cgtttttcct ttcaggcaca ccagttcggc gaccagcagc
     1981 cacgggatca tgccgctgtc gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt
     2041 tcaccaccgt agatggcgtc ttccttgcgc atccgttctt taataaaggc gtgtccggtt
     2101 ttcgacatca ccggcgtgcc gcctgcggca gtcaccacat caacggtgtt ccaggagaga
     2161 cgcggatcgt ggatgatctt cgcgccggga tttttttcga ggaatgcttc tgccagcagg
     2221 ccgacaatgt agtagccctc aataaactgc cctttttcgt caaacaggaa gcagcggtca
     2281 aaatcgccat caaaagcaat gcccatatcc gcaccgtgtt tgatgaccgc attgcgggtg
     2341 tcgtcgcggc attccggcag tagcgggtta ggaataccgt tggggaaatt gccgtccggc
     2401 gtgttgtgca ctttgattaa ttccacgggt gcgccaaggg ctttaaagcg ggcttcgatg
     2461 gcgtccacca ccggacccgc tgcgccattc ccggagttaa tcaccagctt gagcggcgtg
     2521 aggtttttga cattgatata accgaacagg tgatcaacgt aagcgtcacg cagattgatt
     2581 tgctgatagc gaccgcgttt ggtttcatca acgggaggaa agtcgttggc ttctgccaga
     2641 cgctggacat cgcgcagtcc ggtatcaccg ctgatcgggc gagccccttc gcgcaccagt
     2701 ttcatgccgt tgtaatccat cgggttatgg ctggcggtaa cttcaatgcc gccatccacg
     2761 ccgagatgga acgtggcgaa atagatctct tcggtgccgg acatgccaat atccagcaca
     2821 tcgacgcccg catcctgtaa acctctcgcc agcgccagtt ttaaggtttc gctggtgagg
     2881 cggacatcac cgcctaacac aatggttttc ggtttgagaa attcgccata ggcgcgacca
     2941 atgcgccagg cgatatcttc attcagttct tcgcctaatt tcccgcgaat atcataggct
     3001 ttaaagcagg ttaatttttt catgtcgtta ccctttttca ggcaatagtt ggccctgacc
     3061 gaaacggcca attttgtttt tgtcgttatt tacatctcgg tttcgcattc attgcctgat
     3121 gcgacgttta cacccgtccg tagcgatccg cgaaacgcac cacatcatcc tcttcgagat
     3181 aagagccgga gcgcacttca attaaatcga gcggaatttt ccccgggttt tccaggcaat
     3241 gcatcgcccc cagcggaata taaatggact cgttttcacc aagcagtttg atatcaccat
     3301 caatggtgac ttttgccgtt cccgcgacaa ccacccagtg ttccgcgcgg tgatggtgca
     3361 tctgtaccga caagccctcg cccggtttca cggtgatgcg tttcacctgg tagcggtcgc
     3421 ccgcgtcgat agagtcatat ttgccccacg ggcgatagac ttcgcgatgc acccgatgct
     3481 catggcgacc atcggctttg atctgctcga ccactttttt cacatcctgc accgcgttac
     3541 ggtcggcaat cagcaccgca tctttggtct gcaccactac cagatctttc accccgacgg
     3601 tggttacgag gccagattcg gcgtacacat agctgttttc agttttgtga ttaatcacat
     3661 cgccgtggca aacgttgccc tcggcggtgt gggcgctaat ttcccataat gaggaccagg
     3721 agccgacatc gctccagccc gcatccatcg gcaccacaac ggcatctgcc gtacgttcca
     3781 tgaccgcgta atccaccgac tcttccgggc aggcgagaaa cgcttcttca tccacacgaa
     3841 taaaatcgag atccggatcg acggcgctca tcgctttttc acaggcatcg agaatatccg
     3901 gacgatattt tttcagttct tcgagatagc gtccggcgcg gaacaggaac atgccgctgt
     3961 tccagtaata ttcgccgctt gccacatagg cctgggcggt ttccagattc ggtttttcga
     4021 caaactgcgc cacttcaaag gccaccgtat cctgctcacc cgcagacact tcaccgcgac
     4081 gaatatagcc ataaccggtt tctggtagat ccggcacaat gccgaaggtc accagcttgc
     4141 ccgcttcggc atatggcatg gcattacgca cggcggcacg gaacgcgtct tcatcggcaa
     4201 tcacatgatc cgccgccaat accagcatta acgggtcgct ctccgggcta tgacgttttg
     4261 ccgccagcgc cgccagcgca atggcaggtg ccgtgtttcg ccctgccggt tcgagaataa
     4321 tgttctcggt aagtttgttc agttgacgca gctgttccgc gacaataaag cggtgctgct
     4381 cattgcaaat caccaccggg ctttcgcact ccacgccgtt caggcggcag atggtggttt
     4441 gcagcatggt gagatcgcct ttcaggcata aaaactgctt ggggtaaagt acgcgggaaa
     4501 gcggccataa gcggctaccg gagccacctg ccatcacaac tggatagagt ttcgactgcg
     4561 ccataattat ccccgaatat catttataaa ttgacgtaac acgttctctt tatcgagtgt
     4621 gcgttcggca tattcacgtg ccaccgtgtt gtgtttgggc agcaggagcg cctgacggat
     4681 ccccgccacc agcgcctcga ccgattccgg ttctacgcaa accgcaatgc ccggaaaggt
     4741 ttcgcaaagc tgccccagtt ctgtgtgggc ctctgcggta atcaccgcgt taccgcctac
     4801 cgccagaata ttggtcagtt tcgacggcaa tacggcatct gccgcgccgc gtttttgcac
     4861 caccagatgg caatcgccca tcttcagcag tgcgggtaaa gcgtcatacg attgcagcgg
     4921 gaaaaattgc atgttgcgca gtccacgctg ctgcgccatt ttttccagcc gcgctttgcc
     4981 gccgccctgc ccgacaatgg caaaaatcag cggttcatcg cgcagacgat cggcagcttc
     5041 aataacgttt tccagcccct gcttttcacc aatattgccg gagtaaagaa tgattttttt
     5101 gttatccggc aggtcaagct ggttacgaag ggcgtcaaca tcggcatctg caacatgctg
     5161 aaaacgggca atttccgacc agttggggaa gaagatgacg ttttccgccg ccacgccttt
     5221 ttcgatggct ttattcatca tcgaacgcga aatcgtggag acgttatcga cgttatgcag
     5281 tccgctacgt tcgaacgccg ttgccagctg tgccactttg ctgcctttgc cttttccggc
     5341 aaggcccagc cccagcatgg cgtccacttc gtaatcctga atatgcagca cggtacgcgc
     5401 accagaaagt ttcgccagca ggcgcattcc cggcgtgcaa aacagcgttg gcaccacgcc
     5461 gataatgcga tccggcttcc agcgacgttg cgccatcaga ggaaagaaac tgctgacggc
     5521 aaaactgccg agatgtaaca ggcgtttcag ggtgctcggc tgttttggca cgtacagcgg
     5581 gcagcgccac accgtggcgg ccccctcttc tcgtttgtac cgccaggcgg aatagttctc
     5641 gcccacctgc cactgcgggt agtaaggcgg tgcggtaata acccgcacct catgaccttg
     5701 tgccgccagc cattccacca tctcgccggt gtatttgccg ataccggtta actccggcga
     5761 gtagttaatg ccgtagacca gtattttcat aatccgggta ctccggcacg cttctcagcg
     5821 agaaaatagg cgcggctgtt agcgtgaaca tcgttactgg cgagcagcgc gtccggcgtc
     5881 agccagcggt aatcgtcatg ctgctcatcc ggcagtatca gctcttcttc agcgactctg
     5941 aagcgaaaac cgagcaccac atagtgagtg gtgaaatccg tgccggagaa gttatcgtca
     6001 taaaagtgct gccagacacc gtaaaactgg cctgctgtta tcggcagacg cagccccagt
     6061 tccgccatcg tcagccgctc gaatgcggct tccagcgttt cgtctttctg cacgcgccct
     6121 cccggcacaa accagtaacc ctgcgccggg cggttggttc ttttgccaag cagaaactca
     6181 ccgcgactgt tctcgacaat aaagtcgaga gagacaagcg gggtggagcg cactaccgtg
     6241 gcaaagtctt cctgacgtaa aaacatcatt acccccgaaa gcggtcttga ttctcaagga
     6301 accactggta agtgctggca agccccgctt ccagtgagat ttcgtgatac cagccaagct
     6361 gatgcaggcg cgtcacatcc agcagtttgc gcggcgtacc atccggtttg ctggcatcaa
     6421 aaaccacccg acctttgtaa cccaccactt tggcgatggt ttgcgccagc tcgcggatag
     6481 tgcagtcaac gcccgtgccg acgttaatgt gcgacagcat cggctgggtg ttctccagcc
     6541 agacttcgtg cgccagctcc atgacatgaa tgctcgccgc cgccatatca tcgacgtgca
     6601 ggaattcacg catcggtgta ccgctgcccc ataccaccac gtccggtgca ttctgtgccg
     6661 tcgcctcgtg gaagcgacgc agcaatgcag ggatcacatg cgaattactc gggtggaagt
     6721 tgtcatgcgg gccatacagg ttggtcggca tgactgagcg gtaatcgcgt ccgtactggc
     6781 ggttgtatga ttcgcacagt ttgatcccgg cgattttggc aatggcgtaa ggctcgttgg
     6841 tcggctccag cgtgccctgc aataactcgc tttctgccat cggctgtttt gccagtttcg
     6901 ggtagataca ggacgatcca agaaacagca gtttgttcac gtcgttctga tgcgcggcgt
     6961 gaatgatgtt gctctcaatc atcatgttct ggtagatgaa atccgccgga taggtgttgt
     7021 tggcaacaat gccgcccact ttcgccgccg ccagatagac ctggtcaatg cgttcgctgg
     7081 caaagaaatc atgcaccgcg cggctgtcca gcaggttcag ctcgtcgcgg gtgcgtaata
     7141 ccagttccac atcaccgcgc tgttcgagct gccgcctgat ggcagaaccg accatcccac
     7201 gatggccagc gataaaaatg cgttgtttac tcatgcttat gactccagcg cgatcgccac
     7261 gtcgtagccg tgagatttca gcagagagtg ttttttcgcc gcttcgaggt cattagccac
     7321 catttcagac accatctctc tgagggtgat ttccggtttc cagcccagtt tttcgtgcgc
     7381 tttggtcggg tcgccgagca gcgtttcaac ttcagccgga cggaagtaac gcggatcaac
     7441 ggcgataatc acatcacccg gtttaacgcc cggcgcgtca tgcccggtga cggaaaccac
     7501 aatgcccttc tcttcaacac ccgtgccttc aaagcgcagt ttgatgccca gctgtgccgc
     7561 tgccatttcc acgaactgac gcacggagta ctgaacgccg gtcgcgataa cgaaatcttc
     7621 cggctgttcc tgttgcagca tcatccactg catttttacg tagtctttgg cgtggcccca
     7681 gtcacgcagg gaatccatat tgccgaggta caggcacgac tccagcccct gggcgatgtt
     7741 ggcgattgcg cgggtgattt tgcgggtaac gaaggtttcg ccgcggcgcg gggattcatg
     7801 gttgaagaga attccgttac aagcgtacat gccgtaggat tcgcggtagt taacggtgat
     7861 ccagtaggcg tacagtttgg cgaccgcata cggagatcgc gggtagaacg gcgtggtttc
     7921 tttctgcgga atttcctgca ccagaccata cagttcagag gtggaagcct gatagaaacg
     7981 agttttcttt tccagaccga ggaagcggat cgcctccagc aggcgcagcg tacccatcgc
     8041 gtcgacgtca gcggtatatt ctggtgactc aaaagagacc gcaacgtggc tcattgcgcc
     8101 caggttgtac acttcatccg gctgtacttc acgcaaaatg cgcgtcaggt tagaggtatc
     8161 actcaggtcg ccataatgca gatggaattt cgggttgcag gtgtgcggat cctgataaat
     8221 gtgatccacg cgctcggtgt tgaatgacga tgcgcgacgc ttaataccat gcacctcgta
     8281 acctttttcc agcagaaact ctgccaggta agaaccgtct tgtccggtta caccggtgat
     8341 gagagcgact tttgacatgt cttattcctc tgtatttttg aatttattca gtttcaacgc
     8401 gttcgcgtat caccactgcg ggattccccc ggcaaaccac atttgccgga agcgatttaa
     8461 aaacactgct tcgtgcaccc acgacggtgc cgtcgccgat tgtgacgcct ggggcaacaa
     8521 agacatcggt tgccagccag catttctcgc caatcacaat aggcgtggcg ttaatggtga
     8581 aatgttgact tgcatggtcg tggctaccgg tgcataaata acttttttgc gatatcaccg
     8641 aatgtgcgcc aatggttatt tcaccgaggg tatataaatt gacgtcatcg ccgacccacg
     8701 cgtaatcacc taaggttaat ttccacggat aggtaatttt tactgacgga cgaataacta
     8761 cgttttttcc tatttttgct ccgaataaac gtaataaaaa agcccgccag cgatacaata
     8821 cttgtggcga ccaggcaaat attgttgcct gtactgccca ccataatttc actttaatag
     8881 cgttgccgcc ccggaaccct ttcggcaccg agaaaccgct taaatcttgc attggttttc
     8941 cttatattca gactttgtta tataaggctt tcgtctttga ggtagtacgt tggcgtaaat
     9001 gccaggataa ttcagcccaa aagccaggca catgtaatat ttgtcgttgg acttttttcg
     9061 cgtcagcaca caattccata ttattggtgg tagatacccc acccatggaa aattcagaca
     9121 ccaggccatt gagtttttta aatgcataac cggctttata cattttggct gccagcgcgt
     9181 agtcggaaga aactttatat tccaggtcat aacgccattt tttcaagccg gatacaggga
     9241 aaaatatcgc ctgatgactg gcgggcaggc tgtgataaat ataccagccc ggtttggcgc
     9301 tacgtttaat tttatgcccg tcgccaaaat ccagcagcgc atcgccggtg atcatcatgt
     9361 tatctttttg catttttaac ttacggacaa aatttgcggc atcctgatga aaaatatcgc
     9421 ccgaattgag aaacaacgcg aacttgcctt gtgccatcgt aatgcctttg ttcatggcgt
     9481 cgtagatacc gttatctggc tcgctgacaa agcgtaggtt atagatacca ttgagatttt
     9541 ccagatactc acgggtgccg tcattggaac cgccatcgac aacaatccat tcgaagctga
     9601 tatcttccgc ctgcgccaga tgcgccagcg aggcatgtgt tttgactatc ccttcgaggt
     9661 tacgaaacgc gacagtgatt atgctaagca acatgttctt attcttacct cgtaatattt
     9721 aacgcttttc gcaaaataaa cggacagacg attaaaaatg catattccgg gctaaatatc
     9781 gaaccggtaa aaaacagcga taccggagta aaaagatata attgcacgcg aaaattgcgg
     9841 ttatcaccaa aagcgttgat catcattttt atcactttcc ccatgtacca cagcgataaa
     9901 aacactgcga accaggaaaa ataaataatc agcagataca aaccattgtc tatggttttt
     9961 ccgacatccg caccgttaaa tattccgaat gatgcgacgt attcataaag tgagccaaat
    10021 ctgactacac cgtcaatatg ggtcaaagaa taaccgacca tcaccagcgg accgacaata
    10081 cgataatacg atgacgatcc ttccgtacct aaatcgccca gacgggtgga aatataagga
    10141 aacgcgatta ccacaccaac ccggaataca gccagagaaa tcaatgccaa cggtaacttt
    10201 ttcttaatcg cctctttatt cagatattga aatgcccact ccagcaaata aaacaggata
    10261 aaagtcataa cccctgaaaa cgatccggat aatattatcc ctgcgagaat catagcatcg
    10321 gttttaggcg ttttgatacc aaactgtttg atgctgagcc aaattgagat taatgccaga
    10381 gcgaaaaatg ccggttcgaa ataaagtgct gtcgtgcgct tgccgccgaa tttaatgaaa
    10441 ttcagtacat agctgttgct gtaaatcaga tatttcgaaa tcgactccat aatactgctg
    10501 ccaccggtga ggataatttg cgccatctcc accgccgcca gcgccaccac cagcccgatc
    10561 accagataaa agaaacgtaa tatcttgcga tggttgtgtg gcgaaatcgt tttaaagcga
    10621 atactccaca ccatgccaat aatgatcaca atatagacaa acagcatggt tgaagtgacg
    10681 tatttactgg catccagcga ctgaccaaac agatagttaa acgccgtgag tcccgcacca
    10741 atccctaagg caatcatcaa ttttttaacg ctgatgcgtt ctaaaaacag cagtaacaag
    10801 acaggtaaaa aagtgacgat ggtgatgggg aaactttcgc caagctgggc gattttgacg
    10861 ttaaccagca ggtagatcag cggcagcagc aggtagctac agattctgat agaagttgac
    10921 atactcctcc agcatctgtt gtccactgta ggtggcgcgg ctgcgttggc tgaactcagc
    10981 cagcgtggta ccaaatatcg cctgcgcgat ttccggtttg cttaactgca ccagttgcag
    11041 cacgtcttct tcgtcgacgg ttttaccgcc ggatttttgc aacacttccc gtgccgcgtc
    11101 gctatgggtg gcaatcaccg gcacgccaat tgataacgcc tcacacaaaa tcagcgggta
    11161 gttatcgacg cgagaactga ataccagcgc atccatctga ttgagcgcgc tcatcaactt
    11221 gcgcttgtca gtttcaaagc cgtgattaac cacgttgcca gcggtgaacg gcgagaactt
    11281 accaaaggta tgcagttcga ttttgtcgcc cagcgccatc atctcacgca ccagttgctg
    11341 gttagtcttg ccgtcgtaac gcaagtcatg cgccaccacc gcgattttcg gcttgccctg
    11401 ggtttcgcgc accggaggca agtccgccag aatcgcttcg gttgccatat caataccatt
    11461 attgataatc cggcaacgcc ctggaccgta caggctattg aaagcgtcag ccacatgctg
    11521 gctgggggaa ataaactgac agcccagcgc cagcatctca cggaataact ggcgtttgcc
    11581 cgccaccagc tggtgtgcgc gatcaatctt caccggcgga tagttattta aggtcgggca
    11641 tttctggcag cccgttttcc agccttcgca accgtcggta aaggcgcagc gtccggtaac
    11701 actccagtgg tcgtgcagcg tccagaccag agtgacgtcc ggtttatggt ttttcacttt
    11761 ttcgcaaaag cgcaccacgc tcttaagatt tagccagtag ctgtgcagca catgaaaatg
    11821 caggaccacc ggacccggtg tgcgagtaat ggtgcgatat aactcattga aattgccaaa
    11881 cagatcgcga ttaaacagac gaaacagggc aatattcgcc atcgcggtca tccgcggcgt
    11941 atgtttgatg acctgcggat agttctgatg gctgacgctc tctttgccgc ctttgccata
    12001 accgtagaca aaatgtgacg ccagcccctg ttgcagcgca cgctggtgga gatctaacgc
    12061 cacacctgct gccccgcctt ccgccagtcg cacattaaat tgcagaatat tcatttaatt
    12121 accttcactc gcgctttttc tcccaccacc agcgcgttgt ccgggacaga gtcgagcacc
    12181 acgctgcccg cacccacggt gacgttgtta ccaagcgtga tatcaccaag aataatgacg
    12241 ttggcaccga gttcgacgcc gttgccaatg tgtggacatg ccatgttatc ggcaccacga
    12301 ttgccgatag tgacgccgtg gcgaatggtg aaatcatccc ccgctaccac gtttttattg
    12361 atcactacgg cgtaaccgtg atggatagta aagcggcggc caatggtcgc ggcggcctgg
    12421 atttcataac cgaaaaagca ttcggtgata atgcgataca gcaccagcag cggggccgcc
    12481 cacagattgt tgaggacgtt ctttttgcgc cacaccgaac aaaaatgagc aacacgatag
    12541 gcaagaacca tgcagcacgg gcgtaaactc cagctgttgg cacgcagatc ttccagcatc
    12601 tttagcgccc ccgaatacca tcagccagac gtttgccgtt acgcaccgac agcagcgtca
    12661 gcaaagtgcg ccaggtcata cgtttattgc ggatctggta gagggtaaac agctgatatt
    12721 ttttgcttgc acggtcaaat ttatctttgt gctttcggta aaagtggaag tacccggaga
    12781 attttttcgg tgacgaggtg atctgcattt caccgtgatt gatatgcagg atctgcgtcg
    12841 cttcttccac tttccacggt tcgccgtact ccaccaccat ccgcaggaag atgtcgtaat
    12901 cctgtgctgc tttcagttcg gtatcgaaca aacactcttt gaaacgccat gcccaagtaa
    12961 agacctgatt gccaatgata ttgcgtttat agaacaggcg gcgtgagtac ggcgatttgg
    13021 gatacagtgg taagcttgcc ggttgggaat aaacttctcc ctggcatacg tagtcgttag
    13081 cgtacaaaaa ggcgtgagtg accagttgct gtttatgggc gaggaagacg ctcagacggt
    13141 tgggtgtcca ttcgtcatcg tcatcgatcc cggtaatgta ttccccttgc gccagcataa
    13201 tcgcctggtt acgtaccgcg cacgccccgc tgttaatgtc gttgtgaatg taggtgatac
    13261 gcggatcgtt gagggcagtg acgtactgtt gcagttgttc ccaggaggtg gagcaatcat
    13321 ccacgatgat catctcccag ttgctgtagt cctggcgcag gaccgatttt atcgcccgaa
    13381 tcgccagttg ttgacggttc caggtcggca tatagattga aatcagcgga ttctctttca
    13441 taggttgttc tcccggctta tgagctggtt tgcttgccgg atgcggcgtg agcgccttat
    13501 ccggcctacg gggcggtgcg aatgcaggcg tcgttatttc gcatccgact tatattcgta
    13561 ttcgtaatag ccataatcct gatacgcgct ggcgcggcgg aagatggagt tcagaatcac
    13621 ccctttcacc ggaataccgt tttgctcaaa gcggctcaga ctggtttcca cttctttcaa
    13681 tgtgttgacc gcatagcgcg ccaccattaa cgtggttcca gcgtgacgcc cgacaattgc
    13741 tgcatcggtc actgccagaa tcggcggcgt atcaatcaac accaggtcgt agtttttact
    13801 cgcccagctc accaattcgg caaagcgttc gctcatcaac agttcagaag gatttggcgg
    13861 cacctgaccg cgcgggatca ggtcaaattt ggcaatagag gtcggtttag ctgcggtagt
    13921 aatatcgccc tggccaatca gaatttccga caggccattg acgttattgg tacccaacag
    13981 ctcgtgggtg tagcctttgc gcatatcgca gtcgatcaac aacacccgtt tattggtctg
    14041 actgatcacc gccgccaggt tggcgcagac aaaggtttta ccgattgacg ggctcacccc
    14101 tgtcatcatc aacacattgt tctgcgcctg catcatcgcg aagtgcagac tggtacgcag
    14161 actgcggatg gcttcaatcg ccagatcggt tggattcccc accgccagta gctggctctg
    14221 tttatagcgt ttaatccctt tgatggtttt gacgctatcg cgcgctttct gccattccga
    14281 cagcgggatg ctggcataga cgctgatacc atgttcttcc agcacctgcg ggctttcgat
    14341 gccgcgatta aacaacgagc gcagcagcac acccacgata gagagcatca ggccgaggat
    14401 aattgcgccg aggataatca gtcctttctt cggtttcagc acgccaggct gggtgattgc
    14461 cgggtcaaca atgcgcacat cgccgacggt gctggcttcg gtgattttca gctcctgctc
    14521 tttattcagc agttgcatat agacctgctg accagactcg acatcgcggg tcaggcgcac
    14581 aatctcctgc tgggttttcg gcatcgccgt tacgcgaccg ttaagtttgg ctttctcttc
    14641 ttccagcgcc tgacgtttct ccagcagcgt gcggtacgcc gggtgaactt tggtgtacag
    14701 cttggagatt tccgcctctt taaaggtcag ttcgttcaac tgcgcgtcga tgttcaccat
    14761 cgaatcgagc accgctttcg cttccagcgg cagatcaaca gaatctttat cctgacggaa
    14821 ggcattcagt ttgttttcgg caacatcgag gcggctacgt acttccggta actgttgcgc
    14881 gaggaaagcg aggcttttcg acgcttccgc cgatttgcgc tcaatatttt gttcctgata
    14941 gttacgggcg atgctgttaa gaatgtcgcg gatctgttcg cgatcttcac cggtataagt
    15001 caggctcagt acgcctgcgt ctttgccgtt ctccgttacc gtcaggctgt tttgcagttg
    15061 gttgatcatc cccaacgtgg agtatttggt gacggtaaac tcactgcccg ggcgggcgtg
    15121 aatggcttca accatcagcg tgacgccttc ttttttcagc atctggcccg cttgcccacg
    15181 ggcgctaaag ccgccatcac tgctcagggt gtagttttta ttgtccagca cattaagcgt
    15241 aaacacctgg cccgccatct ctttcgggcg gttaaaggta gtcactttca ccgtctcgtt
    15301 ctggcgtccc atcagtcgct cccagcccgc cccgaaaatc gggaacgtgt ttttgctcac
    15361 tgcaatatcg aggtcgagat cgtccaccgt tttaccaagc accagacgcg aacgaatcaa
    15421 ctggatctcg gcgtccgatg caggcggttt gttggctaat gccgaaccaa tgtcctgcac
    15481 taacgaattg ccgctgtttt gctcgatttg taccagtgcg tcggcactat aaatcggcgt
    15541 tgcgaagaag gtgtaaacca cggcacagag ggcaaataca gcggtgatgc caatcaccca
    15601 ccagcgcgct tcaatgacgg tgccgaccag gcgaccaata tcgatttcat cactgcccgt
    15661 taccggagcg gcatgttgtt ttactttttc tgtcattctt atacctgctc tgcgttcaat
    15721 gcctgcgccc actggcgggc agaccgttca agtaatgtgt acaccgctgc aaacgtttcc
    15781 cggcttttgc gatacggatc ggggatttca cattcgttat cccagtgacc aaacagcatc
    15841 actttgccgc gcatctcagg tgccatctcg cataagcgtt cgatatggcg cttttccatg
    15901 gtcaaaatca ggtcatagtt gcgacacagg cggcggctga tttgtcgtgc acagtggcct
    15961 tccagagaca gttgatgttc tgcggcgacg ctgatagcgg taggatcagc gcccttaccg
    16021 accagcgcgc cgagtccagc ggactccact ttcagttccg ggtgataacg ttgcagtaag
    16081 cgttccgccg tcggggaacg gcaaatattg ccgacacaga caactaatat gttgttaaac
    16141 atgacgatta ccagttatga atgtcgctgg ctgtatccgt catgtaacgg acaccgctaa
    16201 tagttggcaa caactgattg atcagacggt tccagcggga aaccggggcg gtggtgacat
    16261 acaccacgtc ataaggttgc aggcggaatt ctgtcgccat caccagcgac gtggcatcgg
    16321 acatatccag ctggtagata ttggcaatct tgccattacg cccgccctcg cctttcagcg
    16381 gacgaatgac aaagatgccg ctggcgttgg aggtggtcat gtcgatacct tcagcattac
    16441 ccagggcttc agtcagggtc atgccgctaa agtccatttt cagggtgctc tgtttcttca
    16501 cttcacccat cacaaatact ttcagatcat cattacgcgg cacgtagaga atatcgccgg
    16561 gataaagcag tcggttctga ttgaggtcgc cgttttgcat cagcgcctgc aaagaaatgc
    16621 gttcttcacg accattgtgt gttagcacta cgttgcgcca gtcagcggtg tcggtcaggc
    16681 cacctgcggc gttgatggcg tcgagaatag tcagtggcac gttggtgatc gcctgttgac
    16741 cggatttatt cacctgaccg gagatatagg ccttttgtga gcggaaggcg gcgatattaa
    16801 cgtccacctg cgggtcagcg atgtacgtcg ctaagcgccc ggtaatatca ctgcggattt
    16861 cagcgagcgt tttcccgact acgtgaacct tgccgatata cgggtaaaac atagtgccgt
    16921 caggctgtac ccagttgccg gtgtcgctgg agctgcggta ctgaccggct ggcgtggtga
    16981 gttccgggtg atcccagacg gtgacattca gaacgtcgcc cggcccgacg cgatactggt
    17041 aattcgcgat ctcgctttcc agcgtcatat tggggcgcgc tacattcggg cgtgggcgta
    17101 attggtcaat caggcgcggg gtcagcggat aaacattcac cattttgtcg agatcgaaat
    17161 cagcgtcctg ctgtttgatg acgtctttgc ccatcgtcga catattgctg cccggaagta
    17221 ctgtgcaacc gcttatcaag gttactgaca ccaataatgg catcaatttc attttggatt
    17281 tcatcattgt ttatttatca ctttggcaga gtaattatcc tgtgcactat taatagcaat
    17341 atcgccatgc acatttacct tgcagttaat tgaataaaaa tttaactggc atcagtccta
    17401 aaaaaattga tttcatccgc aggctattga cagaataatt cagactggtc tttcaggcat
    17461 ccagacacgc taccgcccct ggctttttag ccaccaacac actgatttag cttaattttt
    17521 caaaccctct ctgcatacaa tcgttgatga gaaagggtta ttgcggaaat taacttccga
    17581 atataaggtg acattatggt aattgaatat tggctctcca ataatgcagg aggaagtgtt
    17641 acagctaacg gaatagcagg caaggtaaca attcggcaat tggctatttt taagaattat
    17701 attaagtgtc attcaatatg gtttttagga gtttctttag gttgacaata tttaatattg
    17761 cgcctccaca tgcgatattt cttaaataat gttttattat taccactttt aattcaggga
    17821 taatgtgagg ttattaccct caaataatat gagataaata gtgctgcaac attgcatttt
    17881 tgccccgatt tatccatgat cgaattgtga catttgtcat acaacgaata ggttttgtac
    17941 ttactatgga atggattgcc gatccgtcta tctgggccgg gttaatcacg ctgattgtga
    18001 tcgaactggt cctcggcatt gataacctgg tctttattgc catcctcgcc gaaaaactac
    18061 cgccgaggca gcgcgaccgc gcacgggtta ccgggctgct gctggcgatg ttaatgcgcc
    18121 tgttactgct ggcgtcaatc tcctggctgg tcaccctgac tcaaccgctg ttcagcttcc
    18181 gctcgtttac ctttagcgcc cgcgacttaa tcatgctgtt tggtggtttc ttcctgttgt
    18241 tcaaagccac gatggagctg aacgaacggc tggaagggaa agacagcaat aatcccacac
    18301 aacgcaaagg tgcgaagttc tggggcgtgg tgacgcaaat tgtggtactg gacgccattt
    18361 tctcactcga ctcagtgatt accgccgtcg ggatggtcga ccatttactg gtcatgatgg
    18421 ccgccgtggt tatcgctatc agcctgatgt tgatggccag caagccgtta acgcaatttg
    18481 ttaacagtca cccgacgatc gttattctct gcttaagctt cctgttgatg attggcttta
    18541 gcctggtggc agaaggtttc ggcttcgtca ttccgaaagg ctacctgtac gctgccattg
    18601 gtttctcggt gatgatcgag gcgctcaatc agttggctat ctttaaccgg cgacgttttc
    18661 tttccgctaa ccagacgctg cgccagcgga ctaccgaagc ggtaatgcgc ctgcttagcg
    18721 ggcaaaaaga agatgcggaa ctggacgccg aaaccgcgtc catgctggtg gatcatggta
    18781 accagcagat ctttaatccg caggaacggc ggatgattga gcgggtactt aatcttaacc
    18841 agcgtaccgt cagcagcatt atgacgtcgc gccacgatat tgagcatatc gatctcaacg
    18901 cgccggaaga agaaatccgc cagttgctgg agcgaaatca gcatacgcgg ctggtggtta
    18961 ccgacggtga tgacgcagaa gatttgctcg gtgttgttca cgttatcgac ctgctacaac
    19021 agtctctgcg cggcgaaccg ctcaacctgc gggtgttgat tcgccagccg ctggtgttcc
    19081 cggaaacctt gccgttgtta cctgccctgg agcagttccg taatgcccgc acgcactttg
    19141 cttttgtggt ggatgagttt ggctcggtgg aagggattgt gacattaagt gacgtcactg
    19201 aaaccattgt cggtaactta ccgaacgaag tggaagagat cgacgcccgc catgatattc
    19261 agaagaatgc ggacggttcc tggacggcga atggtcatat gccgctggaa gatctggtgc
    19321 aatatgtgcc gctgccgctg gatgaaaaac gtgaatatca caccattgcc gggctgttga
    19381 tggaatattt acagcgtatt ccaaaacctg gcgaagaagt tcaggtgggg gattatttgc
    19441 ttaaaacgtt gcaggtggaa agccatcgcg tgcagaaggt gcagattatt ccgctgcata
    19501 aggatggcga gttggagtac gaggtgtgat ggtatcggtc attttaccca atagccgaaa
    19561 ggaataagat gagggaaccg cgccagcggc gatttaccgc cgggagcccg ggttgccagg
    19621 gtggtggcga ttgagccacc ctggcacgtt caccggcttg agcgttccat agtagcgagg
    19681 aacatcaagt gaacggaaca gccttcgatc ttacatattc agaatataat gatgccgcca
    19741 cgaacgcctg atgcgacgct ttacgcgtct tatcaggcct acaggactgg tcacctggta
    19801 acctacatct tctccagcaa cttcttcaca tccttgccat tgcgggaatc tttattccgc
    19861 tcggcccagt cattcaggcg acgtttcgct tcgtcctgta gatgtttgcg cagtaactga
    19921 tccacttgca ggctgtaatt gagttgctgc cagttgccat aaacccgcag cggtactggc
    19981 gtttctttca ggaaatcaat cagtttactt tccccgttcc agccacccac gacccgaata
    20041 tcaaactggg tatcgcaggt ttgatctgcc agattcaaca tgccttcccc tgtcagcgcc
    20101 agcacaggcg attgaccttg catgtcgttt aacgtcacga cgccatcctt caacgtcaaa
    20161 tcggtggtaa agcggtcaag ccgcgtcacg ttatcaaagt tttcagcggc cttcacatca
    20221 ccgccattac gctctaccgc ttgctgaatc atctgctgga agttcatccc ttccatgcgc
    20281 gtgtcggtca tttcgacatg tgcctgtcct tgccagttgt ggcggaatgc gtcggcatct
    20341 atgtcagcac cggagaaatc accagccagt gacatttttc cggtcaacga aatcggatag
    20401 ttaaacgcct tcagaatggt accaatctca acgttttcca gccgtggctg gaagtttatc
    20461 cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg aaacctgtcc accgttaagt
    20521 ttgccctgca gttgagtaat ttccagcaaa cccgacttgt tggtcatttg cgtggcaaca
    20581 tctgtaaaat tcattccgcg ccagcgcacg ttactggcct gcaacaaaat atcagccgta
    20641 aagccttgca gtccctgata ggccggttca tcaatacgcg aagaaatgac cgggcgcggc
    20701 aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc ggtttcatta
    20761 agcgggatga ggttgtcaag attcagttgc gggaattgca gtctcagctg ccattccggt
    20821 ttctctgtca gcgtgacctg tgcttgcccg ctcagcgtac tgtcattggc ggtcaagctg
    20881 atttggttaa atgaaaggcg tttatgtgac tcctgccact gggcctggaa actcccctgc
    20941 ccctgaatac cttgttttgg taaatcggca ccctgcaact gccagttaat ttgttcaata
    21001 gccgccgtta aatcatgcgg ataatcagaa gcatccaccg taccgttaag ggatattgtg
    21061 agatcgcgct gatcgcgatt aacccgcccg gagaactcaa atgagccacg atgttgggga
    21121 tcttgttcca tttgcaggcg gatattgcgg attgtcacct gctcgtcatc ttcatgctgg
    21181 aacaccagca cgctgtccgc caccttaaga ctggatatat caaacgacca tccgcgatcg
    21241 tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc actgcgcacc
    21301 gcttccgtct gcggcgtcag ttggatcact gcccctttta gcatcacctg cttaacgctc
    21361 agttgatgac tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc ggcgcgaacc
    21421 agtggctggc ttgcgccctg ggcggtgaga gacattcgcc cggagaggat actaagctgc
    21481 ggccagacgt gccaacgcag tggcccgtcg agctgcaatt gataaccgct acgtgcagca
    21541 acttgcttga ccatatagtc gcggaaatca ttcggattca ccagcaacac taacacagat
    21601 aacccggcca ccagcacgac caggagtatc atcagcgtcg tcagaaatcg tctcatggta
    21661 tcctcaatgg gcctgaatta gtctttatcg attcggctgg ctaccgcgcc ctgctggttg
    21721 cgatatttcg catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga aagcggctca
    21781 aagctcagcg caccaattaa catgcccgga cgcagcgcca gcggcagctt accggagttg
    21841 tagaactcca gcacaatgca accagaccag cccggatcga tacggtgcgc ggtgacgtgc
    21901 accatcagcc ccagacgcgc caaagaggaa cgcccgtcca gccagcccac cagatcggct
    21961 ggcagcgtca ccgactccag cgtcaccgcc agcgccagct ctcctgggtg aagatagaac
    22021 gcctcgccct cgtcgagaac gatctcatcg ctcatcacgc ggtcaagcgc ggcgctcact
    22081 tcatctttgg gaccgctcag atcgataaac gctgccgtgt gaccacggaa ggtacgaaat
    22141 ttattgccca ggcgtacatc caccgtcgcg ccgttaatac gctccactgg cggacgtggg
    22201 ttgatcgaca aacggccttc atcaagccag gcttcaatat ctcggtcaca cagacgcatg
    22261 gcattttctc ctttcgcgca tcactccctt aacgccaatt acgtcaaggg cataccaggt
    22321 tatcactgaa cggtacacaa tttatcaagc ttattcaaag aactgactta ttttcgcttt
    22381 caatatatcg atcgcgatgc ggtttttccc gccgcgcggc acgataatgt ccgcatattg
    22441 tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg gttttttgat attgcgccat
    22501 cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc gcatcaggca
    22561 gatatccagc ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca aacgcgcatc
    22621 cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc ggctcaaccg tcaccgtttc
    22681 tttcatacgc gtatgttcaa catagctgta aaccggcagg tcaattgccg agccgcgttt
    22741 caacgcttgt aaatgctcaa gcagcaggct gtgatccatc gcgctgggat ggtcgtagtt
    22801 ggtcttaacg cgttcttcca tcgacagatg gctttgatct ttgtaatagc agtcttcggg
    22861 aattacgccg atgtgttcat caccgacttg ctcacgtaat tcacgataaa gggtactggc
    22921 aataagactc ttgccggaag ccgatgcgcc agcgataccg ataatgacgc actgatgaga
    22981 ctgatcagtc atatatttag cgacctgatt aacctggatg ttaggaaggg ggcgacgaag
    23041 cgccaaacgc ggcaattata gggatttcat ccgtctgata ccagtcgaat agcgttgccg
    23101 cgcgctcaga gttaattgtt gacaaagaat tcccggtggc aaattacgtt gatcagtttt
    23161 atgtaaggta aaaaatgtta tacgcagttg cgcaaattat ccgcctttac gtcactttat
    23221 gagcaattcg catataaaat gtaaaacttt tgtactagca taaacacaga aacgaatact
    23281 ggcgacctgg tcttgcggat aaagcggtaa tgagcaaaca atcacagcat gtattaattg
    23341 ccctgcccca cccgctgctt cacctggtca gtttaggttt agtctcgttt atctttaccc
    23401 ttttctcgct tgagctttcg cagtttggca cccaactcgc cccactgtgg ttcccgacgt
    23461 ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat gtggccggga attgcgctga
    23521 gctgttcgct gggaaatatc gccgcatcca tcctgctttt ttccaccagc tcgctgaaca
    23581 tgacctggac gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg ctacgtaaat
    23641 tgctgccgtg gtataacccc ttgcaaaatc tggctgactg gctgcgtctg gcactcggca
    23701 gcgccattgt tccgcctctg ttggggggtg ttctggttgt cctgctgacg cccggagacg
    23761 atcctctcag ggcatttttg atatgggtac tgtcagaatc catcggcgct ctggcactgg
    23821 tgccgctggg attgttattt aaaccacact atctgctgcg ccatcgcaac ccacggttgc
    23881 tttttgagtc gctgctcacg ttagccatca cactgacgtt aagctggctt tcgatgctgt
    23941 atctgccgtg gccttttact ttcattattg tgctgttgat gtggagcgcc gtgcgcctgc
    24001 cacgaatgga agcctttttg atcttcctta ccacggtgat gatggtgtcg ctgatgatgg
    24061 ccgcggatcc ctccctgctt gctacgccgc gtacgtacct gatgagccat atgccgtggc
    24121 taccgttttt gctgatcctg ctgcccgcca acatcatgac catggtgatg tatgcctttc
    24181 gtgcggaacg caaacacatt tccgaaagcg aaacccgctt tcggaacgcg atggaatatt
    24241 ccgctatcgg tatggcgtta gtgggcaccg agggacaatg gctgcaaacc aacaaagcgc
    24301 tctgccagtt tctcgggtac agtcaggaag agctgcgcgg actcaccttt cagcaactga
    24361 cctggccgga ggatctcaat aaagatctcc aacaggttga aaagctgata agcggtgaaa
    24421 taaacaccta ttcaatggaa aaacgctact acaaccgcaa tggcgatgtt gtctgggcgt
    24481 tgcttgccgt ctcactggtg cgccacacgg atggcacgcc gctctatttt atcgctcaga
    24541 ttgaagacat taacgagcta aaacgcaccg aacaggtcaa tcagcaactg atggagcgca
    24601 tcacgctggc taacgaagcg ggcgggattg gcatctggga gagggagttg aagccgaatg
    24661 tttttagctg ggataagcgg atgttcgagc tgtatgaaat tcctccacat atcaaaccga
    24721 actggcaggt gtggtacgag tgcgtgctgc cggaagatcg ccagcatgcc gaaaaagtga
    24781 ttcgtgattc gttgcaatca cgctcgccct ttaaactgga atttcgcatt accgtaaaag
    24841 acggtattcg ccatatccgc gccctcgcca accgggtact gaataaagaa ggcgaagtcg
    24901 aacgtctcct cggcatcaat atggatatga ccgaagtgaa acagcttaac gaggcattgt
    24961 ttcaggaaaa agagcgcctg cacattacgc ttgattccat cggtgaagcc gtggtctgta
    25021 ttgatatggc gatgaaaatt acctttatga atccagtggc ggagaagatg agcggctgga
    25081 cgcaggaaga agcgttaggt gttccgctcc tgacggtgtt gcatattact tttggcgaca
    25141 acggaccatt aatggagaac atttacagtg ccgacacctc acgttccgca atcgaacaag
    25201 atgtggtgtt gcactgtcgg agcggcggca gttacgacgt gcattacagt attacgccgt
    25261 taagtactct ggacggcagc aatattggtt cggttctggt gattcaggac gtcaccgaat
    25321 cacgcaaaat gctgcgccag ctgagctaca gcgcctccca tgatgcactg acgcatctcg
    25381 ccaaccgcgc cagttttgag aaacaactgc gtatcctgct gcaaacggta aacagtacgc
    25441 atcagcgaca tgccctggtg tttatcgatc ttgatcgctt taaagcggtg aatgacagcg
    25501 ccgggcatgc ggcgggcgac gctttgctgc gcgaactggc gtcgttgatg ctgagtatgc
    25561 tgcgctccag tgacgtgctg gcgcggctcg gcggtgatga atttggtctg ctgctgccag
    25621 actgcaatgt tgaaagcgct cgttttatcg ctacacgtat tatcagtgct gtgaatgact
    25681 atcactttat atgggaaggc cgggtacatc gggtaggtgc cagcgccggg attaccttga
    25741 tcgatgacaa caatcatcag gcggcagaag tgatgtcgca ggctgatatc gcctgttatg
    25801 cctccaaaaa tggtggacgg ggccgggtga cggtttacga accacagcaa gctgccgcac
    25861 atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg gatgattaaa gagaatcagt
    25921 tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc ggaagcgcgt aatttgtggc
    25981 tgatttcgct taagctctgg agttgcgaag gcgaaattat tgatgaacaa acatttcgtc
    26041 gtagcttcag cgatccggca cttagccatg ctcttgaccg acgggtattc cacgaatttt
    26101 tccagcaggc cgccaaagcg gttgccagta aaggcataag catcgccctc cccctttccg
    26161 ttgccggttt gagtagcgcc acgctggtga atgatctgct tgagcagctg gaaaatagcc
    26221 ctctaccacc acggttatta catctgatta ttccggctga agcgatttta gatcacgcag
    26281 aaagcgtgca aaaactgcag ctggcgggat gtcggatagt gctcagccag gtgggccgcg
    26341 atctgcaaat cttcaactcg ctgaaagcga atatggcaga ttacctgcta cttgatggtg
    26401 agttatgcgc caacgtgcag ggtaatttga tggatgagat gctgattacg attattcagg
    26461 ggcacgctca gcgactcggg atgaaaacca tcgccgggcc agtcgtttta cccttagtga
    26521 tggatacgct ttctggcatc ggcgtcgatc tgatttatgg tgatgtgatt gccgatgccc
    26581 aaccgctgga tttgctggtg aatagcagtt atttcgcgat taactgagag agcgtttgcc
    26641 cattgcctga tgcgacgcta acgcgtctta tcatgcctac aaatcgctca ttccccagac
    26701 cggataaggc gctcgcaccg catccggcga ccaacgtcat gcttcgtctg gttgccagcc
    26761 ttccgtatac cagatatgca acagcgcata agaacgccag ggcttccagc gctcggcata
    26821 acggcggatt tgtgccggtg tcattcccgg aaatcgccgt ttaatcagat aatcatccgg
    26881 cagaaaaaca tctttcgcct gccagccacg caaagcaaaa taattcgccg tccagcgccc
    26941 gatacccgga aaagtttgca gcgttttcat cgcctgctcc acatcgcccg gaattgtcat
    27001 tggtaagctg ccctccagcg ccgcatttgc cagatgaatc agtgcctctg cccgtttcaa
    27061 cggcatgcct aacgctttta atgcctgcgg gtcggctgct gccagccgct gaggcgtggg
    27121 gaagcagata tactccggaa aatcatccag ccgttcgcca taaagctgta ccactttggc
    27181 agtcaatttt gccgccatcg ccacgctcac cagttggcct aaaatcgccc gcacaccctg
    27241 ctcaaaagca tcaatacagc cgggtaaacg caatccgggc cgccccgcgc ctaactttcc
    27301 caacgcaccg ttaacaatct gcgggttaca ttgcagatca agcaggcggc tcattttcgc
    27361 cagacactct gcggcaacag gttctaaacc tgcacttaaa tttatgtgca gagtatggcg
    27421 ggctatatcc ggaatagcag tcactacgcc gcgatattcg cccaccgcca gactacgggc
    27481 ataataattg tccgcgaccg tttccacacc gctcaccgca cgggcggcga gaaatcccaa
    27541 catccacgac cagtcatacg gcggctgcca gttcagggta tacatcgcat ctccttgtta
    27601 atccgctttc agcataaacg ttattcagac gttacgcttt gctttcatat tccggttgtc
    27661 gcgacagcaa catttcgcta aagtcacgcc ccttcttcac cggcatgggg attaattcgt
    27721 gtttattggt tttgattacg gtacagcaaa ctgttcagtg gcggtcatgc gtgacggtaa
    27781 accgcaatta ctgaaaatgg aaaacgacag cacgctgctg ccttcaatgc tttgcgcgcc
    27841 aacgcgtgaa tcggtaagcg aatggctgta ccgccatcat gatgttccgg cagacgacga
    27901 tgaaacgcag gcgctgttac gtcgggcgat tcgttataac cgcgaagaag atatcgatgt
    27961 tacggcgaaa agcgtgcagt tcggtctttc ctcactggca cagtacattg atgatccaga
    28021 agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt gccagcggct taaaaccgca
    28081 gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg ttgcacattc gccagcaggc
    28141 gcaggcgcag ctgccagaag cgattactca ggcggtgatt ggtcgcccga tcaacttcca
    28201 ggggctgggc ggcgatgaag caaacgccca ggcgcaagag attctggaac gcgcggcgaa
    28261 gcgtgccgga ttcagggacg tggtattcca gtacgagccg atcgcggctg ggctggatta
    28321 cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg gatatcggcg gtggtacgac
    28381 tgactgttca ttgctgctga tggggccgca gtggcgttcg cgtctcgatc gtgaagccag
    28441 tctgctgggt cacagtggtt gtcgtattgg cggtaacgat ctggatatcg ccctggcgtt
    28501 taaaaacctg atgccactgc tgggtatggg tggcgaaacc gaaaaaggca tcgccctgcc
    28561 gatcctgccg tggtggaatg cggttgccat caacgacgta cctgcgcaga gtgatttcta
    28621 cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc gatgcccgcg aaccggaaaa
    28681 agtggccctg ttacagaaag tctggcgtca gcgtttaagc tatcgcctgg tgcgtagcgc
    28741 agaagagtgc aaaattgctc tttcaagcgt agcggaaacc cgcgcctcac tgccgtttat
    28801 cagcaatgaa ctggctacgc tgattagcca gcgagggctg gaaagcgccc tcagccagcc
    28861 actggcgcgg attctggaac aggtgcaact ggccctggat aacgcccagg aaaaaccgga
    28921 cgttatctac ctgaccggcg gtagcgcccg ttctccgcag attaaaaaag cgctggcaga
    28981 acagttgccg ggcattccga ttgcaggcgg cgatgacttt ggctccgtca ccgccgggct
    29041 ggcacgctgg gcggaagtgg tgtttcgtta attgaaaaat ggctcagcag cggcttaaat
    29101 ctgcctgcgc ctgagccagt ttttctgctg cctgacgtaa cggttccatc aacggcatgg
    29161 tccgtgctgg agcatgaagg cggaatcgct gcaactctgt cgcgccgact gtcaacgccg
    29221 attccagccg atgtcgtttg gcggtcattt tcgccattac cgcttgtctg tctgctggcg
    29281 ttatcgcttc attcggcctg aacacaaaac ggcgttcaca gttcgctttc cagtcgataa
    29341 ccgcctgggt cagatggtcg ccaaaccctt tcacttgctt aacgccacga cgggtaacat
    29401 ctgcggctgt ttcaatacca aaagaccgta acgccgcttt acgcgcaggg ccaacgccgg
    29461 gaatagaggc aacatcaata aaaaatccct ccagaaactt ctgcttctgc cgttcccttg
    29521 cagtgtcatg aagtgctgcc agagcgcgtt tctcttcttc cggtaacccg agaatttcgt
    29581 ccttcatttt ttcgagcatc gtccgtttgg cgataaaacc ttccaggccg cctacctgct
    29641 ggatctggcg gactaaatgg tcatagtcca ttttcgcacg attaaatgcc tcacggcgtt
    29701 gctgaacttc tgctttgtac gctttgcttg tcaaactgcc aataatccag atagccgcca
    29761 gcacaggaac caaaataata taacgcggtt ctgcctgaag gccgcaaagc agcagcgata
    29821 atgctgacag tgcgatctca agcagaatga tgtattcgcg ccgtaacagg cctaaaggaa
    29881 gcggcctgcc agtcggttgg aaatgatcgg gtaatggcag ttgcaatgct ggcggtgcta
    29941 ctgacgccat caccatcgcc cagacttttg ccagcacaaa atccccgccg gtggtaatga
    30001 cctcttcgcc gagatcaata aaatagataa caccttgatt atccagcgca caccatgggc
    30061 agtcagccaa atgaccggga taaacatgca ttgccgaaac ggtacatttc tttaactgtt
    30121 ggcgtagaga atccagtgct gatacccacg ctttagccgt cggacgcccg gttgccacgc
    30181 cactttccgt aaacgcctgc tgaaacatgg cttcaacatc actcggtaac atcgatagcg
    30241 gaatcgatcg tggcggcggt tttaaaccac gtcgctgatt atctgacgcg taggcataac
    30301 ggaaatgggt aatatccgtc tccagcgcat tacccgcatc agagataagc ggcacaccag
    30361 aataaggatg ccggccacca aacaagacgt gaaaaatcag caacgcaagg ccaaaattat
    30421 cgtgattttc tgtgcgttca aagccgacaa atgacgacaa ggtttgtagc tctggcggcg
    30481 taaaatgcga cacgccgact tcgcataaat gcagtgtgcc attggcgtta atctgaaagg
    30541 agtcgctatc aatcaacacc actttgctgt cgcgacctac cataaaactg ttctggttta
    30601 cgtcacccac gacgtgcccg tgctcgtgaa ccgtagcaaa agatgaagca atattgcgcg
    30661 caacatagag tagaaaatcc cacgcacaat gagggtaacg ttgacgacga tgtgccgggc
    30721 tatagatcat atgaatcggt tctttaccag aaacttttgg catcataaaa ccgataactt
    30781 ttccgcctcg tccaccgtga agcgttgcct gcggccaggc gacataattc aacaattgcg
    30841 cgtcagctgt cgcagccata aaggcaagtt tgtcctgttt taaggcgggt ggcggcgtgt
    30901 gataaatctt ggcgacgcta tcgacaaact cattgatatc ataaaccgcg ccttcaccgc
    30961 ctttgcccag ttcacggcct ggcgtaacgc actcaccagt agcagtatat aaagtgggtt
    31021 tcatggtaac cacagcgcca gtgccaacgt tttatcatca tccgtacgct cgtttaccgc
    31081 cgggctggac aaaaactgct ttaacaattc aggtaataaa tcgagttgtt cctgcgttgc
    31141 tgacgccagt ccattgaaaa acggggtaaa gaaaggcaca tgaggagaat tatccagcat
    31201 atttaacgcc agccgctgaa taccatccgt aaatgctgca actttatgcg cacgcccagt
    31261 gctggtgaaa gtttccagtc tggaaacggc atcttcgtcg gtaatgaagt gcgtcatgtt
    31321 ggcatattca cccaccatcg gcgtgagagg caattgcaga ccgtgaccca aatctaccac
    31381 cacgccgcca tcgccaatct gcataatcaa ggtgccgtca ggcgatgaaa tcaatcccag
    31441 aaacgtgcag gcaaaatcgc gaactgctaa tgctttggct tctgcctcgg caaaaagtcg
    31501 ttgacgaata gtcagtacca tatttgtggc gaggacatca ttaagcccca attcaccgtc
    31561 ctgtactttt tgcaccataa acgccatcgc ttcattgacg gcgagcgtcg cgccttctcc
    31621 tccctgcgaa acgctacctg cgccatcagc aaggaacatc accagtaatg gttgctggtc
    31681 gtttaaccag acaacctgca tctggcaggc atcctgacaa ggcaaatcag cactaatgtg
    31741 cgatgttcca acagcggatg cataaaccag acgccagctc acactgacgt ccagcctttt
    31801 ggcgcttcca gcacaacttc cgttcccggc gtggagcggg aaaccgaacg cagcgaactg
    31861 gataaccagc taaacagttc acggaattgt aatccctgca gtggcaaagg ctgacgaacg
    31921 ctgatttgtg ccagagtctt catatccgcc ccctgaacac cgatggaaaa gaaggcaaac
    31981 ttcttatctt cttccccctg aaacactttg ttggcggcag cctgccactc atcggttgga
    32041 gcgccatcgg tgatcaggaa aatccacggg cgataatacg aaataccatt tgcgcggtac
    32101 tcacgtttcc tctcttcaac catatccagg gctttggtaa tggcggcccc cataggcgta
    32161 tccccctggg caaacaggat gggcgggaaa aagttagcgg cgctggtaaa gggttgttcc
    32221 acatgcaccg ggccgaacgt cacaatgccg agttccactc tttttaaagc cagcggatca
    32281 gcaagcagtt catcacgaaa ggtaaccaat ccggcgttaa gttcattaat aggtctgccg
    32341 ctcatcgagc cagaaacatc cagcagcaaa atgcaggggc aacgtggttc cgggttactg
    32401 gcaaaatcgc tggtggcaaa cgtgatttgt tcgctcataa gaaatccttt cttagagaat
    32461 tatcgaggtc cggtatcata ccgattgcaa taatgaaata agagcaaacg aagaaacttt
    32521 cagaattagg cccgtcggcg cagaaaattt tgctattcgc ctcgcaagaa cggagagccg
    32581 tcgattgaca ctaagggcgg agtgacataa tttcaggagt gagggttagg gagaggtttc
    32641 cccctccccc tggtgttctt agtaagcctg gaagctaatc actaagagta tcaccagtat
    32701 gatgacgtgc ttcatcataa ccctttcctt attaaaagcc ctcttctccg ggagagggtt
    32761 tcccgtttca gcgtcccgct gaaatcgtcg gcttacctcc tttcgccctg aatgcagtct
    32821 atcgctaacg cgtgaacaaa atagtttcat ctgtgattat tgcgtaacga ctcgcaaaga
    32881 atatttgctg gcaggatcgc agactacaaa gcctgcggat tgacaatctt atcgtgaagg
    32941 cataatttca ggagtgaggg tagagcgggg tttcccccgc cctggtagtc ttagtaagcg
    33001 gggaagctta tgactaagag caccacgatg atgagtagct tcatcatgac cctttcctta
    33061 ttaaggcccc ttcctcggga ggggctttcc cgtttcagcg tcccgctgaa atcgtcggct
    33121 tacctccttt cgccctgtat gcagtctatc gctgacttgc ggataaaata gtttccagtg
    33181 tgattattgc gtaacgtctc gcaaagaata tttgctggca ggatcgcaga ctgcaaagcc
    33241 tgcagattga caatcttatc gtgaaggcat actttcagga gtgagggtag agcggggttt
    33301 cccccgccct ggtagcctta gtaagctggc cagctaatga ctaagagcac cacgatgatg
    33361 agtagcttca tcatgaccct ttccttattt atggcccctt cctcgggagg ggctttcccg
    33421 tttcagcgtc ccgctgaaat cggcggctta cctcctttcg ccatgcaagc agtctatcgc
    33481 taacgcgtag ataaaatagt ttcctgtgtt attactggat gcgtgctcgc aaatgtgccc
    33541 gtcattcaga cgattcccga cagtgtttca taattcctcc atttttctcc cttattggct
    33601 ggctacacta gtatcattcc gcgaaacgtt tcaggaagag aaactcttaa cgatgaaagg
    33661 cagttataaa tcccgttggg taatcgtggt ggttatcgcc gccatcgccg cattctggtt
    33721 ctggcaaggc cgcaatgact cccagagtgc agccccaggg gcgacgaaac aagcgcagca
    33781 atcgccagcg ggtggtcgcc gtggtatgcg ttccggccca ttagccccgg ttcaggcggc
    33841 gaccgccgta gaacaggcag ttccgcgtta cctcaccggg cttggcacca ttatcgccgc
    33901 taatactgtt acggtgcgca gccgcgtgga cggtcaactg atggcgttac atttccagga
    33961 aggccagcag gtcaaagcag gcgatttact ggcagaaatt gaccccagcc agttcaaagt
    34021 tgcattagca caaacccagg gccaactggc aaaagataaa gccacgcttg ctaacgcccg
    34081 ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc gtcaggagct
    34141 ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc attaaggctg atgaagcaag
    34201 cgtcgccagc gcgcagttgc aactcgactg gagccgcatc accgcaccag tcgatggtcg
    34261 cgttggtctc aagcaggttg atgttggtaa ccaaatctcc agtggtgata ccaccggaat
    34321 tgtggtgatc acccagacgc atcctatcga tttagtcttt accctgccgg aaagcgatat
    34381 cgctactgta gtgcaggcgc aaaaagccgg aaaaacgctg gtggtagaag cctgggatcg
    34441 caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc aaatcgatgc
    34501 cacaaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg cgctgtttcc
    34561 caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg tagtgatccc
    34621 aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga atagcgaaaa
    34681 caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag tggtgatccg
    34741 tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc attgatcgcc tgaccgaagg
    34801 ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga aagccaccag
    34861 ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc gagcagcaca
    34921 ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct gatggtggcg
    34981 atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct gccggaagtg
    35041 gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga tgtcatgacc
    35101 tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct gaaacagatg
    35161 tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct aacattaccg
    35221 ctcgatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa cttgttgccg
    35281 agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc gccgatcatg
    35341 acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaagatat ggtggaaacc
    35401 cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct ttccggcggt
    35461 cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct cggcctgacc
    35521 agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa aggtagcctc
    35581 gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc cgccgaagag
    35641 tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg cgatgtcgca
    35701 actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa agaacaggcc
    35761 attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc cgacagcatt
    35821 cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt gacagtgctt
    35881 tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga attgatgatg
    35941 gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc ggcgaccatc
    36001 attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat ggtgtttctc
    36061 gatttttcaa tcaataacct gacactgatg gcgttaacta tcgccaccgg attcgtggtc
    36121 gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg cgaaaaaccg
    36181 ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc gctgaccttc
    36241 tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg gcgactgttc
    36301 cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt gtcgctgacc
    36361 ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa acagaaccgc
    36421 ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg tcgtggactg
    36481 gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac gctgctgctt
    36541 agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga caatggcatt
    36601 attcagggca ctttgcaggc accgcaatcc agctccttta ccaatatggc ccagcgacaa
    36661 cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac ctcatttgtt
    36721 ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa cctcaaaccg
    36781 ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac ggcggtagat
    36841 aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg atctgactat tgatactcag
    36901 gtcagccgca cccagtacca gtttaccttg caggccacct cactggatgc gctcagtacc
    36961 tgggtgccac agttgatgga aaaactccag caactgccac agctttctga tgtctccagc
    37021 gactggcagg accaagggct ggtggcgtat gtcaatgttg atcgcgacag cgccagccgt
    37081 ctggggatca gcatggcgga tgtcgataac gccctgtaca acgcgtttgg tcagcgactg
    37141 atttccacta tttatactca ggctaaccag tatcgcgtag tgctggagca caacaccgaa
    37201 attaccccgg gcctcgcggc gctggatacc attcgcctga ccagtagcga cggcggcgtg
    37261 gtgccgttaa gctcaattgc caaagttgaa cagcgttttg cgccgctttc catcaaccat
    37321 ctggatcagt tcccggtaac gaccatctcc tttaacgtgc cggataacta ttcgctgggc
    37381 gatgcggtgc aggagattat ggacaccgaa aagacgctga atctgccggt ggatatcacc
    37441 acgcaattcc agggcagcac cctcgccttc cagtcggcgc tgggcagcac tgtctggctg
    37501 attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga gagctttatt
    37561 cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct gctggcatta
    37621 atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt gctgatcggt
    37681 attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga gcgcgagcaa
    37741 ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg tccgatcctg
    37801 atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac cggggtcggc
    37861 gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt cagccaggtg
    37921 ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc attgtggacc
    37981 aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc cctcttcatt
    38041 taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg catactgggc
    38101 ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat tatggtcagc
    38161 gcctcgctgc ccggtgcgtc accagaaaca atggcgtctt ccgttgccac gccgctggag
    38221 cgctcgcttg ggcgcattgc cggggtcagc gaaatgacct ccagcagttc gctcggcagc
    38281 acacgtatta ttttacagtt tgattttgac cgggacatca acggcgcagc gcgtgatgtg
    38341 caggcggcga tcaacgctgc acaaagtttg ctgcccagtg ggatgcccag ccgtccgacc
    38401 tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac atccgatact
    38461 tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac gatttcgcaa
    38521 atcgacggtg ttggtgatgt cgatgtcgga ggcagctcac tgcccgccgt acgcgtcggg
    38581 ctgaatccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg caccgccatc
    38641 agcaatgcca acgtgcgtaa accgcagggc gcgctggaag atggcactca ccgctggcag
    38701 atccagacca atgatgagct aaaaaccgcc gctgaatatc agccgttgat tattcactac
    38761 aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgaccgactc agtgcaggat
    38821 gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat ccgcaaactg
    38881 ccggaagcca atattatcca gacggttgac agcatccggg caaaattacc ggagttgcag
    38941 gaaaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc caccattcgc
    39001 gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt gattctggtg
    39061 gtgtttttat tcctgcgctc gggtcgcgcc actattattc ccgccgtttc ggtgccggtt
    39121 tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa taacctttcg
    39181 ttaatggcgc tcaccatcgc tactggtttc gtggtggatg acgccatcgt ggtgctggaa
    39241 aacattgcac gtcatctgga agcgggaatg aaaccgttgc aagccgcact gcaaggtact
    39301 cgcgaagtcg gttttacggt gctgtcgatg agtctgtcac tggtggcggt gttcctgccg
    39361 ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt gacgctttct
    39421 gtcgccattg gtatttcgtt gctggtttct ctgacattaa cgccaatgat gtgtggctgg
    39481 atgctgaaag ccagcaagcc gcgcgagcaa aagcgactgc gtggttttgg tcgcatgttg
    39541 gtagccctgc aacaaggcta cggcaagtca ctgaaatggg tgctcaatca tacccgtctg
    39601 gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct ggctgtatat ctcgatcccg
    39661 aaaaccttct tcccggagca ggacactggc gtgttgatgg gcgggattca ggcggatcag
    39721 agtatttcgt ttcaggcgat gcgcggtaag ttgcaggatt tcatgaaaat tatccgtgac
    39781 gatccggcag tggataatgt caccggcttt acaggcggtt cgcgagtgaa cagcgggatg
    39841 atgtttatca ccctcaagcc acgcgacgaa cgcagcgaaa cggcgcagca aattatcgac
    39901 cgtctgcgcg taaaactggc gaaagaaccg ggggcgaatc tgttcctgat ggcggtacag
    39961 gatattcgcg ttggtgggcg tcagtcgaac gccagctacc agtacacgtt gttatccgac
    40021 gacctggcgg cactgcgcga atgggagccg aaaatccgca aaaaactggc gacgttgccg
    40081 gaactggcgg acgtgaactc cgatcagcag gataacggcg cggagatgaa tctggtttac
    40141 gaccgcgaca ccatggcacg gctgggaatc gacgtgcagg ccgccaacag cctgttaaat
    40201 aacgccttcg gtcagcggca aatctcgacc atttaccagc cgatgaacca gtataaagtg
    40261 gtgatggaag tggatccgcg ctatacccag gacatcagtg cgctggaaaa aatgttcgtt
    40321 atcaataacg aaggcaaagc gatcccgctg tcgtatttcg ctaaatggca accggcgaac
    40381 gctccgctgt cggtgaatca tcagggatta tcggcggcct cgaccatctc gttcaacctg
    40441 ccgaccggaa aatcgctctc ggacgccagt gcggcgatcg atcgcgcaat gacccagctt
    40501 ggtgtgcctt cgacggtgcg cggcagtttt gccggaacag cgcaggtgtt ccaggagacg
    40561 atgaactcac aggtgatcct gattatcgcc gccatcgcca cggtgtatat cgtgctgggt
    40621 atcctttacg agagttacgt acacccactg acgattctct ccaccctgcc ctcggcgggc
    40681 gttggtgcgc tgctggcgct ggagctgttc aatgccccgt tcagcctaat agccctgata
    40741 gggatcatgc tattaatcgg catcgtaaag aaaaacgcca ttatgatggt cgattttgcg
    40801 ctcgaagccc aacggcacgg taacctgacg ccgcaggaag ctattttcca ggcctgtctg
    40861 ctgcgttttc gcccgattat gatgactacc ctggcggcgc tgtttggcgc gctgccgctg
    40921 gtgttgtcgg gcggtgacgg ctcggagctg cggcacctcc tggggatcac cattgtcggt
    40981 ggactggtaa tgagccagct tctgacgctg tataccacgc cggtggtgta tctctttttc
    41041 gaccgtctgc ggctgcgttt ttcgcgtaaa cctaaacaaa cggtaaccga gtaaatgaca
    41101 gaacttcccg acagcacccg ctggcaattg tggattgtgg ctttcggctt ctttatgcag
    41161 tcgctggaca ccaccatcgt taacaccgcc cttccctcaa tggcgcaaag cctcggggaa
    41221 agtccgttgc atatgcacat ggtcattgtc tcttatgtgc tgaccgtggc ggtgatgctg
    41281 cccgccagcg gctggctggc ggacaaagtc ggcgtgcgca atattttctt taccgccatc
    41341 gtgctgttta ctctcggttc actgttttgc gcgctttccg gcacgctgaa cgaactgttg
    41401 ctggcacgcg cgttacaggg cgttggcggt gcgatgatgg tgccggtcgg caggttgacg
    41461 gtgatgaaaa tcgtaccgcg cgagcaatat atggcggcga tgacctttgt cacgttaccc
    41521 ggtcaggtcg gtccgctgct cggtccggcg ctcggcggtc tgctggtgga gtacgcgtcg
    41581 tggcactgga tctttttgat caacattccg gtggggatta tcggtgcgat cgccacattg
    41641 ctgttaatgc cgaactacac catgcagacg cggcgctttg atctttccgg ctttttattg
    41701 ctggcggttg gcatggctgt attaaccctg gcgctggacg gcagtaaagg tacaggttta
    41761 tcgccgctgg cgattgctgg cctggtcgca gttggcgtgg tggcactggt gctttatctg
    41821 ctgcacgcca gaaataacaa ccgcgccctg ttcagtctga aactgttccg cactcgtacc
    41881 ttttcgctgg gcctggcggg gagctttgcc ggacgtattg gcagtggcat gttgcccttt
    41941 atgacaccgg ttttcctgca aattggcctc ggtttctcgc cgtttcatgc cggactgatg
    42001 atgatcccga tggtacttgg cagcatggga atgaagcgaa ttgtggttca ggtggtgaat
    42061 cgctttggtt atcgtcgggt actggtagcg accacgctgg gtctgtcgct gatcaccctg
    42121 ttgtttatga ctaccgctct gttgggctgg tactacgttt tgccgttcgt cctgttttta
    42181 caagggatgg tcaactcgac ccgtttctcc tccatgaaca ccctgacgct gaaagatctc
    42241 ccggacaatc tggcgagcag cggcaacagt ctgctgtcga tgattatgca attgtcgatg
    42301 agtatcggcg tcaccatcgc cgggctgttg ctgggacttt ttggttcgca gcatgtcagc
    42361 gtcgacagcg gcaccacaca aaccgtcttt atgtacacct ggcttagcat ggcgtttatc
    42421 atcgcccttc cggcgttcat ctttgccaga gtgccgaacg atacgcatca aaatgtagct
    42481 atttcgcggc gaaaaaggag cgcgcaatga agttctggcg acctggtatt accggcaaac
    42541 tgtttctggc gattttcgcc acctgcattg ttttgctgat cagtatgcac tgggcggtgc
    42601 gtatcagttt tgagcgtggc tttattgatt acatcaagca tggtaatgaa cagcgattac
    42661 aactgttaag tgatgcgctt ggcgagcagt atgcgcagca tggcaactgg cgcttcctgc
    42721 gcaacaatga tcgctttgtc tttcagatcc tgcgttcatt tgaacacgat aattcggaag
    42781 ataaacccgg cccgggtatg ccaccgcacg gctggcgtac ccagttctgg gtggttgatc
    42841 aaaacaacaa agtgctggtt ggtccgcgag cgccgattcc acctgacggt acacggcgac
    42901 ccattctggt caacggtgcg gaagttggcg cggtgatcgc ctcccccgtt gagcggttaa
    42961 cgcgcaatac tgatatcaat ttcgataaac aacagcggca aaccagctgg ttgattgtcg
    43021 ccctggcaac gttactcgcg gcacttgcca cttttctgct ggcgcgcggt ttactggcac
    43081 cggtaaaacg acttgtcgat ggcacgcaca aactggcggc gggcgatttc actacccgcg
    43141 taacgcccac cagtgaagat gaactgggca aactggcgca agacttcaac cagcttgcca
    43201 gcacactgga gaaaaaccag caaatgcggc gcgattttat ggccgatatt tctcacgaac
    43261 tgcgtacgcc attagcggtg ctgcgcggtg aactggaagc tattcaggat ggcgtgcgca
    43321 aattcacgcc ggagacggtg gcgtctttac aggcggaggt cggtacactg accaaactgg
    43381 ttgacgatct ccatcagttg tcgatgtctg atgaaggcgc cctcgcctat caaaaagcac
    43441 cggtagattt gatcccactg ctggaagtgg cgggcggcgc atttcgcgaa cgattcgcca
    43501 gccgtggcct gaaactgcaa ttttccctgc cagacagtat taccgtattt ggcgatcgcg
    43561 accgtttaat gcagttattc aataatttac ttgaaaacag cctgcgctac actgacagcg
    43621 gcggcagcct gaaaatctct gccgagcagc acgacaaaac ggtgcgcctg acctttgccg
    43681 acagtgcgcc aggtgtcagt gacgagcagc tacaaaaatt atttgaacgt ttttatcgca
    43741 ccgaaggttc ccgcaaccgc gccagcggtg gttccgggct ggggctggcg atttgcctga
    43801 acattgttga agcacataat ggccgcatta ttgctgccca ttcgcctttt ggcggggtaa
    43861 gcattacagt agagttaccg ctggaacggg atttacagag agaagtatga ccgagttacc
    43921 aatcgacgaa aacacaccgc gtattttgat cgtggaagat gaaccgaagc tggggcagtt
    43981 gctcattgat tatctgcgtg ctgcgagcta tgcgccgacg cttatcagcc acggcgatca
    44041 ggtactgccg tatgtgcgcc agacaccacc ggatctgatc ctgttggatc tgatgctccc
    44101 tggcaccgat ggcttgacgc tgtgccggga aattcgtcgt ttttctgaca ttcctatcgt
    44161 gatggtgacg gcaaaaatcg aagagatcga ccgcctgctc ggactggaga ttggcgcaga
    44221 tgactacatc tgcaagccgt acagcccacg ggaagtggta gcgcgcgtca aaaccatttt
    44281 gcgccgttgc aaaccgcagc gcgaattgca gcaacaggat gccgaaagcc cgttgattat
    44341 cgacgaaggt cgttttcagg cttcatggcg cggtaaaatg cttgacctga cgcctgcgga
    44401 atttcgtctg ttgaaaacgc tctctcatga accaggaaaa gtgttctccc gcgagcaatt
    44461 gctcaatcat ctttatgacg actaccgcgt agtaaccgac cgcaccattg acagccacat
    44521 taaaaacctg cgccgcaagc tggaatctct cgacgccgaa cagtcattta tccgcgccgt
    44581 ttacggcgtt ggttaccgct gggaagccga cgcctgccgc atcgtttagt tttagcgaca
    44641 tgactttgtt agccggagat ggcgttcaga ccagatccgg caacattatc ccacgcatgg
    44701 tcagcaaact ga
//
LOCUS       DAZOYL010000004        43274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZOYL010000004 DAZOYL010000000
VERSION     DAZOYL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.004"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            58..576
                     /locus_tag="ACHUOY_000136"
     CDS             58..576
                     /locus_tag="ACHUOY_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0681038.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            592..1131
                     /locus_tag="ACHUOY_000137"
     CDS             592..1131
                     /locus_tag="ACHUOY_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR0681039.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1226..2803)
                     /gene="guaA"
                     /locus_tag="ACHUOY_000138"
     CDS             complement(1226..2803)
                     /gene="guaA"
                     /locus_tag="ACHUOY_000138"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR0681040.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2872..4338)
                     /gene="guaB"
                     /locus_tag="ACHUOY_000139"
     CDS             complement(2872..4338)
                     /gene="guaB"
                     /locus_tag="ACHUOY_000139"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR0681041.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4500..5870
                     /gene="xseA"
                     /locus_tag="ACHUOY_000140"
     CDS             4500..5870
                     /gene="xseA"
                     /locus_tag="ACHUOY_000140"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR0681042.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5867..6082)
                     /locus_tag="ACHUOY_000141"
     CDS             complement(5867..6082)
                     /locus_tag="ACHUOY_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0681043.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6151..7623)
                     /gene="der"
                     /locus_tag="ACHUOY_000142"
     CDS             complement(6151..7623)
                     /gene="der"
                     /locus_tag="ACHUOY_000142"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR0681044.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7741..8919)
                     /gene="bamB"
                     /locus_tag="ACHUOY_000143"
     CDS             complement(7741..8919)
                     /gene="bamB"
                     /locus_tag="ACHUOY_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR0681045.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8930..9550)
                     /gene="yfgM"
                     /locus_tag="ACHUOY_000144"
     CDS             complement(8930..9550)
                     /gene="yfgM"
                     /locus_tag="ACHUOY_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR0681046.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9568..10842)
                     /gene="hisS"
                     /locus_tag="ACHUOY_000145"
     CDS             complement(9568..10842)
                     /gene="hisS"
                     /locus_tag="ACHUOY_000145"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR0681047.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10953..12071)
                     /gene="ispG"
                     /locus_tag="ACHUOY_000146"
                     /gene_synonym="gcpE"
     CDS             complement(10953..12071)
                     /gene="ispG"
                     /locus_tag="ACHUOY_000146"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR0681048.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12098..13111)
                     /gene="rodZ"
                     /locus_tag="ACHUOY_000147"
     CDS             complement(12098..13111)
                     /gene="rodZ"
                     /locus_tag="ACHUOY_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR0681049.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13396..14550)
                     /locus_tag="ACHUOY_000148"
     CDS             complement(13396..14550)
                     /locus_tag="ACHUOY_000148"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR0681050.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14700..15131)
                     /gene="ndk"
                     /locus_tag="ACHUOY_000149"
     CDS             complement(14700..15131)
                     /gene="ndk"
                     /locus_tag="ACHUOY_000149"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR0681051.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15280..17592)
                     /gene="pbpC"
                     /locus_tag="ACHUOY_000150"
     CDS             complement(15280..17592)
                     /gene="pbpC"
                     /locus_tag="ACHUOY_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR0681052.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17593..22554)
                     /gene="yfhM"
                     /locus_tag="ACHUOY_000151"
     CDS             complement(17593..22554)
                     /gene="yfhM"
                     /locus_tag="ACHUOY_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR0681053.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNCLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22761..23606
                     /gene="sseA"
                     /locus_tag="ACHUOY_000152"
     CDS             22761..23606
                     /gene="sseA"
                     /locus_tag="ACHUOY_000152"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR0681054.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23942..24067
                     /gene="timP"
                     /locus_tag="ACHUOY_000153"
     CDS             23942..24067
                     /gene="timP"
                     /locus_tag="ACHUOY_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR0681055.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24099..24875)
                     /gene="sseB"
                     /locus_tag="ACHUOY_000154"
     CDS             complement(24099..24875)
                     /gene="sseB"
                     /locus_tag="ACHUOY_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR0681056.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25017..26300)
                     /gene="pepB"
                     /locus_tag="ACHUOY_000155"
     CDS             complement(25017..26300)
                     /gene="pepB"
                     /locus_tag="ACHUOY_000155"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR0681057.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26478..26678)
                     /gene="iscX"
                     /locus_tag="ACHUOY_000156"
     CDS             complement(26478..26678)
                     /gene="iscX"
                     /locus_tag="ACHUOY_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR0681058.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26690..27025)
                     /gene="fdx"
                     /locus_tag="ACHUOY_000157"
     CDS             complement(26690..27025)
                     /gene="fdx"
                     /locus_tag="ACHUOY_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR0681059.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27027..28877)
                     /gene="hscA"
                     /locus_tag="ACHUOY_000158"
     CDS             complement(27027..28877)
                     /gene="hscA"
                     /locus_tag="ACHUOY_000158"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR0681060.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28894..29409)
                     /gene="hscB"
                     /locus_tag="ACHUOY_000159"
     CDS             complement(28894..29409)
                     /gene="hscB"
                     /locus_tag="ACHUOY_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR0681061.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29505..29828)
                     /gene="iscA"
                     /locus_tag="ACHUOY_000160"
     CDS             complement(29505..29828)
                     /gene="iscA"
                     /locus_tag="ACHUOY_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR0681062.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29845..30231)
                     /gene="iscU"
                     /locus_tag="ACHUOY_000161"
     CDS             complement(29845..30231)
                     /gene="iscU"
                     /locus_tag="ACHUOY_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR0681063.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30259..31473)
                     /gene="iscS"
                     /locus_tag="ACHUOY_000162"
     CDS             complement(30259..31473)
                     /gene="iscS"
                     /locus_tag="ACHUOY_000162"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR0681064.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31714..32202)
                     /gene="iscR"
                     /locus_tag="ACHUOY_000163"
     CDS             complement(31714..32202)
                     /gene="iscR"
                     /locus_tag="ACHUOY_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR0681065.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32503..33240)
                     /gene="trmJ"
                     /locus_tag="ACHUOY_000164"
     CDS             complement(32503..33240)
                     /gene="trmJ"
                     /locus_tag="ACHUOY_000164"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR0681066.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33359..34162
                     /gene="suhB"
                     /locus_tag="ACHUOY_000165"
     CDS             33359..34162
                     /gene="suhB"
                     /locus_tag="ACHUOY_000165"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR0681067.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34307..35161
                     /locus_tag="ACHUOY_000166"
     CDS             34307..35161
                     /locus_tag="ACHUOY_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0681068.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35352..36632
                     /gene="csiE"
                     /locus_tag="ACHUOY_000167"
     CDS             35352..36632
                     /gene="csiE"
                     /locus_tag="ACHUOY_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR0681069.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36624..37763)
                     /locus_tag="ACHUOY_000168"
     CDS             complement(36624..37763)
                     /locus_tag="ACHUOY_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR0681070.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37923..38813)
                     /gene="hcaR"
                     /locus_tag="ACHUOY_000169"
     CDS             complement(37923..38813)
                     /gene="hcaR"
                     /locus_tag="ACHUOY_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR0681071.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38949..40310
                     /gene="hcaE"
                     /locus_tag="ACHUOY_000170"
     CDS             38949..40310
                     /gene="hcaE"
                     /locus_tag="ACHUOY_000170"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR0681072.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40307..40825
                     /gene="hcaF"
                     /locus_tag="ACHUOY_000171"
     CDS             40307..40825
                     /gene="hcaF"
                     /locus_tag="ACHUOY_000171"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR0681073.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40825..41145
                     /locus_tag="ACHUOY_000172"
     CDS             40825..41145
                     /locus_tag="ACHUOY_000172"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR0681074.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41142..41954
                     /gene="hcaB"
                     /locus_tag="ACHUOY_000173"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41142..41954
                     /gene="hcaB"
                     /locus_tag="ACHUOY_000173"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR0681075.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41964..43166
                     /gene="hcaD"
                     /locus_tag="ACHUOY_000174"
     CDS             41964..43166
                     /gene="hcaD"
                     /locus_tag="ACHUOY_000174"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR0681076.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10266 a        11950 c        10859 g        10199 t
ORIGIN      
        1 ttcctctttt gatgatctta aatgtcttat ttttcgtaat gtgtataaca aggaatagtg
       61 atgaaattta aaaaatgtct tctgcctgtg gcaatgttag cgtcattcac tctggcagga
      121 tgccagtcaa atgctgacga tcatgccgcc gatgtttatc aaaccgatca actgaatacc
      181 aaacaagaaa ctaaaaccgt taatattatt tccattcttc ccgcaaaagt tgccgtagac
      241 aactcccaaa ataaacggaa cgcacaagcc ttcggcgcgc ttattggcgc agtcgctggc
      301 ggtgttatcg gccacaacgt cggttctggc agcaattccg gaacgacggc aggggcagtt
      361 ggcggcggag ctgtaggcgc ggcagcgggt tctatggtga atgataaaac cttagtggaa
      421 ggtgtttctt taacatataa ggaaggcacc aaagtgtata cctccaccca ggtgggtaaa
      481 gagtgccagt ttacgacagg tttagccgtt gttattacca caacgtataa cgaaacgcgt
      541 attcagccaa atactaaatg tcctgaaaag agctaataat caggaggagt catgaagaaa
      601 gtttttcttt gcgccatctt agcctcctta agctatccgg ctatcgcctc atcattgcag
      661 gatcaactct ctgctgtcgc agaagcggaa cagcaaggta aaaatgaaga gcaaaggcag
      721 catgacgaat gggtcgcgga gcgcaacagg gaaatccagc aagagaagca acgtcgcgca
      781 aatgcccagg ccgccgctaa caaaagagcg gcaacggcag cggcaaataa gaaagctcgt
      841 caggataaac tggacgccga agccactgcg gacaaaaaac gcgatcaaag ttatgaagat
      901 gagctacgca gcttagagat tcagaaacaa aaactgacgc tggcgaaaga agaagcccgc
      961 gttaagcgag aaaacgaatt tatcgatcag gaactgaagc acaaagctgc gcaaaccgat
     1021 gtggtgcaat ctgaagctga cgccaacaga aatatgactg aaggcggtcg cgatctgatg
     1081 aaaagcgtgg gcaaagcaga agagaacaaa tcggacagct ggtttaatta atcgatgtta
     1141 gtaacttcaa gcctatgatt cttgaagata aaaaaccctc tgtagtaaca gagggttttg
     1201 ttcattcata gtgcagggat taaaatcatt cccactcaat ggtagctggc ggcttgccgc
     1261 tgatgtcata caccacgcgg gaaataccgt tcacttcatt gataatgcgg ttggaaacgc
     1321 gaccgaggaa atcgtacggc agacgcgccc agtgtgcggt cataaagtcg atggtttcga
     1381 cagcacgcag agagacaacc cagtcatact tacgaccatc gcccattacg ccaacggaac
     1441 gtaccggcag gaacacagtg aacgcctggc tgactttgtc gtacaggtcc gctttacgca
     1501 gttcttcaat gaagatggcg tcagcacggc gcagcaggtc acagtactct ttcttcactt
     1561 cacccagcac acgaacgcca aggcctggtc ccgggaatgg gtgacggtac aacatgtcgt
     1621 acggcaggcc cagctccaga ccaatcttac gcacttcgtc tttgaacagc tctttcagcg
     1681 gttcaaccag gcccatcttc atctctttcg gcaggccgcc cacgttgtgg tgagatttga
     1741 tgacgtgtgc tttaccggtt gcagacgccg cagattcgat aacgtcaggg tagatggtgc
     1801 cctgcgccag ccacttcacg tcttccagtt tcagcgcttc ttcatcgaat acttcaacga
     1861 aaacgcgacc gatgatttta cgttttgctt ccggatcgtt ttcgccagcc agcgctgaca
     1921 ggaagcgatc ttctgccgga acgtgaacaa tgttcagacc aaagtgatcg ccaaacatat
     1981 ccagaacctg ctctgcttcg ttgagacgca gcaggccgtt gtcgacgaat acgcaagtca
     2041 ggtttttacc gatagcgcgg tgcagtagca ttgcggttac ggaggaatcc acgccaccag
     2101 agaggccgag gatgacttta tcgtcgccta cctgctcacg gatgcgggct acagcatcgt
     2161 cgataatttt cgctggcgtc cacagggctt cacactggca gatatcacgc acaaaacgct
     2221 ccagcatgcg cataccctgg cgggtgtgag tcacttccgg gtggaactgt acgccataga
     2281 agcgtttttc ttcgttagcc ataatggcaa acgggcagct ttcggtgctg gctacggtga
     2341 cgaagtcgga cgggatagcg gtaactttat cgccgtggct catccagaca tcgagcagcg
     2401 gtttaccgtc tgcggtcagc gcatcttcga taccgcgaac cagtgcactg tcgtttacga
     2461 cttcaacctg cgcgtagcca aattcacgtt cgttagaggc ttcaacgtga ccgcccaact
     2521 gcattgccat ggtctgcatg ccatagcaaa cgccgaatac cggtacgcct gcttcaaaga
     2581 catactgcgg cgcacgcggg ctgttttctt cagtagtact ttccgggccg ccggaaagaa
     2641 taatgccgct tggattgaag tcacgaattt gtgcttctgt cacatcccac gcccacagtt
     2701 cgcagtaaac acccagctca cgcacgcggc gcgcaaccag ttgagtgtac tgagaaccga
     2761 agtctagaat gaggatgcga tgcttatgaa tgttttccgt cattgacgct tattccgagg
     2821 caagtgaaac agataatata aatcgcccga catgaagtcg ggcgaagaga atcaggagcc
     2881 cagacggtag ttcggggact ctttagtaat ggtcacgtcg tgaacgtggc tttcctgaat
     2941 gcccgcaccg ctgatacgta caaactccgc tttagtacgc agttcgtcga tagtaccaca
     3001 gccggtcaga cccatacagg agcgcaggcc gcccatctgc tggtgaatga tctctttcag
     3061 gcgaccttta taagctacgc gaccttcgat accttccggc accagtttgt cggcagcgtt
     3121 atcgctctgg aaataacggt cagaggaacc tttggacatc gcgcccaggg aacccatacc
     3181 acggtaagat ttgtaagaac ggccctggta gagttcgatt tcacccggag attcttcagt
     3241 acccgccagc atggaaccta ccatcaccgc gcttgcgcca gcggcgatag ctttggcgat
     3301 gtcgccggag aagcgaatac caccatctgc gataaccgga ataccggtgc cttccagggc
     3361 ttcaactgcg tcagcaacgg cagtgatctg cggaacacct acgccagtaa cgatacgagt
     3421 cgtacagata gaaccagggc cgataccgac tttaaccgcg ctgcaaccag cttctgccaa
     3481 agcgcgtgca cctgcagctg ttgccacgtt gccgccgatg atttgcagat ccggatattt
     3541 agcacgggtt tcacggatac gctgcaaaac gccttctgag tgaccgtggg aggagtcgat
     3601 cagcagaacg tcaacgccag cggcaaccag cgcgtcaaca cgctcttcgt tacccgcacc
     3661 tgcgccaact gcagcaccaa cacgcagacg gccttgctcg tctttacagg cgttcggttt
     3721 acgttccgct ttctggaagt ctttcacggt gatcatgccg atcaggtgga attcgtcatc
     3781 aaccaccagc gctttttcaa cgcgtttttc gtgcattttt gccagcacca cttcacgggc
     3841 ttcaccttca cgcacggtga ccagacgctc tttcggcgtc atgtaaacgc taaccggctg
     3901 gttcaggtcg gtaacaaaac gcacgtcacg accggtgata atacccacca gttcgttttc
     3961 ttcggtaacg accggatagc ccgcaaaacc gttacgctcg gtcagttctt tcacttcgcg
     4021 cagcgtcgtg gttggcagaa cagtctgcgg atcagtcacc acaccagatt cgtgtttttt
     4081 cacacggcga acttcttctg cctggcgttc aatggacatg tttttgtgga taaagccgat
     4141 accgccttcc tgagccagag caatagccag gcgcgcttcc gttacggtat ccattgctgc
     4201 ggaaagcata gggatattca gacgaatagt tttcgtcagc tgggtgctga ggtcagcagt
     4261 attcggcaga acggtagaat gagcaggaac gaggagaacg tcgtcaaacg tcagagcttc
     4321 tttagcgata cgtagcatgg gcaatatctc gaccagagtg gttaataaat attgccgcgg
     4381 cattatacag agcgtaaccg attgcatcta cccctttttg caaaaaatgc ttgctatccc
     4441 cgaagggcgg gttactatcg actgaataac ctgctgattt agaatttgat ctcgctcaca
     4501 tgttaccttc tcaatcccct gcaattttta ccgttagtcg cctgaatcaa acggttcgtc
     4561 tgctgcttga gcatgagatg ggacaggttt ggatcagcgg cgaaatctct aatttcacac
     4621 aaccggcttc cggtcactgg tactttacac tcaaagacga caccgcccag gtacgctgcg
     4681 cgatgttccg caacagcaac cgccgggtga ccttccgtcc acagcatgga caacaagttt
     4741 tagttcgcgc caatattacg ctctacgagc cgcgcggtga ctaccagata atcgttgaga
     4801 gtatgcagcc ggccggtgaa gggctgctgc aacagaagta cgaacagctc aaagcgaagt
     4861 tgcaggctga aggtttgttc gatcagcaat acaaaaaacc acttccctcc cctgcgcatt
     4921 gcgttggtgt gatcacctca aaaaccggtg ctgcgctaca tgatattttg catgtgttaa
     4981 aacgtcgcga tccttctcta ccggtgatca tctaccccac cgccgttcag ggcgatgacg
     5041 caccggggca aattgttcgc gccattgaac tggcgaatca gcgcaatgag tgcgacgtgt
     5101 tgatcgttgg gcgcggcggc ggttcgctgg aagatttatg gagttttaac gacgaacgcg
     5161 tagcgcgggc gatttttgcc agccgcattc cggtcgtcag cgccgtcggg catgagacgg
     5221 atgtgaccat tgccgatttt gttgccgatc tgcgtgcgcc aacgccgtct gccgccgctg
     5281 aagtagtgag ccgtaatcag caagagttac tgcgccaggt gcaatcgacc cgtcaacggc
     5341 tggagatggc gatggattat tatctcgcca accgcacgcg tcgctttacg cagatccatc
     5401 accgattaca gcaacagcat ccgcagctcc ggctggcacg ccagcaaacc atgcttgagc
     5461 gcctgcaaaa gcggatgagc tttgcgctgg aaaatcaact taagcgtacc gggcaacagc
     5521 agcagcggtt aacacagcgg ctgaatcagc aaaatccaca gccgaagatt catcgcgcgc
     5581 aaacgcgcat tcagcaactg gaatatcgtt tagcagaaac cctgcgcgta cagcttagcg
     5641 ccacgcgtga acgtttcggt aatgcagtaa cgcacctcga agccgtaagc ccactgtcaa
     5701 cgctggcgcg tggatacagc gttactactg ctactgacgg caatgtactg aaaaaagtga
     5761 agcaagttaa agcgggtgaa atgctaacca cacgtctgga agacggctgg atagaaagtg
     5821 aagttaaaaa catccagcca gtaaaaaaat cgcgtaaaaa ggtgcattaa gccagcacga
     5881 attcaacgcg ttttttcgat atcaagccat gcccgtgctg acagaagtaa tctactgcac
     5941 cgcaggcttt taacacctgc aacggttgat ggcagtctgg acaaagagct ttcatttcaa
     6001 taatttcccc gcaggatggg caacgcgcat ggccgttatc ctgatcaagc acatgctgac
     6061 attgtggaca atgcagttcc atatgaattc ctccggtagc catccctcta cattcataga
     6121 gggaatggca gataaaatac ttacggataa ttatttattt ttcttgatgt gcttcatcag
     6181 acgcttacgt ttacgcatct gggttggcgt cagggtgtta cgcttgttcg catacgggtt
     6241 ttccccttct ttgaactgaa tacgaatcgg cgatcccatt acgtccagcg atttgcggaa
     6301 gtagttcatc aagtagcgct tgtaggaatc aggcaggtct ttcacctgat taccgtgaat
     6361 caccacaatc ggcgggttat aaccaccagc gtgggcatat ttcagcttca cacgacgacc
     6421 gcgtaccagc ggcggttggt gatcttcaac agccatcgtc atgatgcgcg tcagcataga
     6481 ggtccccaca cgacgggtgg agctgtcata cgcttcacgt actgattcaa acaagttacc
     6541 aacaccactg ccgtgcaagg cagagataaa gtgcacacga gcaaaatcga taaagcccag
     6601 acggaagtcc agcgtttctt tcacctgctc tttcacttcc tggctcaggc catcccactt
     6661 attcaccaca atgacaagtg agcgcccact attgagaata aagcccagca gcgagagatc
     6721 ctgatcggaa ataccttcgc gcgcatcaat cactaacatc accacgttgg tatcttcaat
     6781 tgcctgcaac gttttgatta cggagaattt ctctacagca tcggtgattt tgccgcgttt
     6841 acgtacgcca gcggtgtcaa tgagcacata ctcacgtcca tcgcgttcca ttggaatgta
     6901 gatactgtca cgcgtcgtgc caggcatgtc ataaacaaca acgcgctctt caccaagaat
     6961 acggttagtg agtgtagact tacctacgtt cggacgaccc acaatcgcca gtttgatcgg
     7021 cagactttgc gggtcgaagt cgtcttcctc ttcttcttcg ccgttctctt ccgcttcaaa
     7081 ttgcgcccag tattcagcgt cttcgtcgac ttcctcttgc ggtgcgagat cttccatcca
     7141 cggcagcagc acacgctcca gcagacttaa tacgccacga ccgtgagatg cggcgatcgg
     7201 gtagatttca cctaaaccaa gcgagtagaa atcaaccact gcctgatcgg gatccagacc
     7261 gtcagttttg tttgccacca ggaaggtcgg tttttcacga gagcgcagat gtttggcaat
     7321 cgcttcatct gccggcatca agcccgcgcg cgcatccacc ataaacagta cgacgtccgc
     7381 ttcttcaatc gccagcagcg actgttccgc catgcgggtt tctacaccgt cttctgtgcc
     7441 atcaatcccg ccggtatcaa tacagataaa ctcacggcct tcaatttccg cacgaccgta
     7501 cttacggtca cgagtcagac ccgggaaatc cgcaaccagc gcatctcggg tgcgagttag
     7561 acggttaaat aacgtggatt ttcctacgtt agggcgcccg acaagcgcga ccacaggtac
     7621 catgtttaaa gcctcatttt tataaatcat cagacaacgc acgctatatt cgcgtcgttg
     7681 ttaaaaacag gaaaacggcc cctgtccagg agccgttttc aaagtgaacg acagagacga
     7741 ttaacgtgta atagagtaca cggttccgtc ttttgcctgg atcagcagtt tgccgtcagc
     7801 ggcaaccggt tcagtctgga aaccggaact atcaactttt tgctgggcaa cgaaacgacc
     7861 atcttcgacg ttaatccagt gcagataacc ttcactgtca cctaccacca ggttgccatt
     7921 atacagcacc ggcgaagtca gcaggcgatg cagcagatcg ctttgtgtcc acagcgtaac
     7981 gccgccatca atggtcaacg ccatcacccg gtcattttga tcgaccagat agatgcgatt
     8041 gccgtcgacg atgaaatcat tcaccgaacc cagttcgcgt ttccacataa tctgaccact
     8101 gcgcagatca agcgccgtca ggttaccatt ataggccaga gcgaaaacaa cgccgttaac
     8161 aacgacggga gtcgtgtcaa catcgctcag acggtcaatt tcggtagaac cggtcgcctg
     8221 ggaaatacgc tgctgccaaa tcatctggcc ctgttccatc agcactgcgc tgacgcgacc
     8281 attatcgccc cccacgacgg ccgcaccaaa agccgttgcc ggcgcagact cgccacgcaa
     8341 agagagcgaa ggcatatcga ggttaactgt ccatttgaca gcgccgtcag cttcgttcag
     8401 cgcttgtaac tgaccgttac tagtgtggat taacaccaga ccgtcgctga ccaccgggcg
     8461 agaaagtgct tcacccgcga ctttagtttg ccatgccaca gtaccatcgc tggtattcag
     8521 cgcgtaaacc tgcgcctttt cgctgccaat gtagacatgc ccaccagaca cggtcacacc
     8581 gccagaaagt aatgcaggct ctttagagaa ccagccatct ttctcggcca ggctgacaga
     8641 ccagatttct ttgccatcat ccgcattcag cgcttttacc aaaccagcgc ggtccgctgc
     8701 atagacaacg ttgtccgcca gtgccggatg aagattggaa tagaagttgc caatgccgct
     8761 accaacggaa gtgctccacg ccgtggtcgg cgtaaactgg ttttcaacgg ttggcaatgg
     8821 ggacatcttt accacatctt cttcgctgtt aaacagcgaa cagccgctta aaagggtaac
     8881 ggaaagcagt cctggcagca gtaatttacg caattgcatc gggtccctct cagatggaca
     8941 aattattaat tttcatctgc atcatttcgc tcagtgccgg agtaacatcg cttttcacgc
     9001 ctgcttccca tgcactacgc gcaccttgct tatcaccttt gctcaacaat gctttaccac
     9061 gcaggtcggc aacaatggca gcccaccctt cacctttgat ggtatcaagg gttttcagcg
     9121 cggcatcagc ctgcttgagc tgtacctgaa cgcgagcaag acgcagattt atcacggctt
     9181 tgagattttc atcactcgtg tctgccagcc cctgttgtaa ctgggcggca gctttctcta
     9241 gttcattttt gtcaacaaat tgctgcgcaa gttccaaaga agccagcgca ccataagtat
     9301 ttttattttc agcagcaaat ttttccgccg ccgggatgct atccggtttg ccttcgctca
     9361 ctgcggtaac cgcattttga taggcaagag aagcggagcg tgcagaatca acctgatggc
     9421 tgttccagta gcgccagcca atcagtgcgc caacgcccaa aatcacccca acagccagtg
     9481 ctttgccatt ttcagcaaaa aagcgtttaa ccgcttctac ctggtcgttt tcgttctcgt
     9541 aaatttccac gctgtccttc tccttcctta acccagtaac gtgcgcaaat gcgcggctac
     9601 gctatcctgc gcaactgccg tttgctcacc agagcgcaaa tccttcacta ctgctgtgcc
     9661 gttagccact tcagactcac ccagcaccac agcaacgcgg gcaccccatt tatcagcacg
     9721 ggcaaactgt ttcttaaagt tgccgccgcc gtggttggtc atcaatttca cgcccggtaa
     9781 ttcatcacgc agacgctcag ctaatgccat agccgcagat tgtgtatcag cacctgaagc
     9841 caccaggtat atatcgacaa caggatcggc tttaaattcc ggattaacgg cctgtactaa
     9901 caatacaaga cgttcgaggc ccatcgcaaa accgactgcc ggtgttgcac gaccgcccag
     9961 ttgttccacc agaccgtcat aacgaccgcc tgcacacacg gtgccctggg agccgaggct
    10021 gttagtcacc cactcgaaaa cggtacggtt atagtaatcc agaccacgca ccagacgctg
    10081 gtttacggtg taagcgatcc ccgcgctctc aagcagtttg cacagaccgg caaaatgctc
    10141 acgagattcc tcgtccagat aatcacctaa tgccggagcg tcgttgagaa gcgcctgcac
    10201 ttccggattt tttgaatcca gcacgcgcag cgggttagtg tacatgcggc gtttgcagtc
    10261 ttcgtccagc ttttctttat gctgctcaag gaatgccacc agcgcatcgc ggtaattggc
    10321 gcgtgcttcc agcgaaccaa tagagttcag ctcaagagtt acgtgttcga agatacccag
    10381 cgcacgccac cagcgggcag tgagcataat cagttcagcg tcgatatccg gaccttgcag
    10441 accgaaaact tcgcagccca actgatggaa ctgacgataa cgccctttct gcggacgctc
    10501 gtgacggaac atcggcccga tataccacag acgctgttcc tgattgtaca gaagaccatg
    10561 ctcgatgccg gcgcgtacac agcccgccgt cccttcaggg cgcagagtca ggctgtcgcc
    10621 attgcgatcc tcaaaggtgt acatctcttt ttcaaccacg tcggtgactt caccaatcgc
    10681 acgtttgaat agcggggtct gctctacaat cggcaagcgg atttcactgt aaccgtagct
    10741 gccgagcacg tttttcagtg tgccttcaat gcgctgccag atggccgttt cgccaggcag
    10801 gtaatcgttc atgccgcgaa tggcttgaat gttttttgcc acgtttattc tctttctgaa
    10861 tataaaaatg aaccctcaac gcttccctca atgtttcggg agccatgcgg gttcaatcat
    10921 acacgggaag cgaggcgctt cccatcacgt tattattttt caacctgctg aacgtcaatt
    10981 cgacgcgctt cgtccagctg actggctttc gcacgaatgc gtgcttccag ctggtcgatc
    11041 atatcgttgt tgtccagacg gtctttgcgc acgccatctt catagaggcc gcttttcttg
    11101 ttgccgccgg tgacgccgag tgtagaaacc agcgcctcac ctgggccatt caccacgcag
    11161 ccgataatcg aaacgtccat cggagtgatg atatcttcca ggcgttgctc cagcgcgtta
    11221 accgtaccga taacatcaaa ttcctgacgc gaacaggtcg ggcaggcgat gaagttaatc
    11281 cctcgcgaac ggatacgcag cgatttcaaa atatcgaaac cgactttgat ctcttcgacc
    11341 ggatcggccg ccagcgatac gcgcagcgtg tcgccgatgc cttccgacag cagcagacct
    11401 aaaccaatgg cggattttac tgccccgctg cgtgcaccac cggcttcggt gatccccaga
    11461 tgcagcggct gatcgatctg ttttgccagc aaacgataag actcaacagc gaggaagacg
    11521 tcagacgctt tcacgctgac tttgaactga tcgaagttca ggcgatcgag atgatcaaca
    11581 tgacgcatgg cggattccag caacgcctgc ggcgtcggtt cgccatactt ttcttgcaga
    11641 tctttttcca gcgatccggc gttaacgcca atacggatcg gaatgttttt atcgcgcgca
    11701 cagtcaacca ccatgcgaat acgctcttca ttaccgatat tgccagggtt aatacgcaga
    11761 caatcgacgc cgtattccgc tactttcagc gcaatgcgat agtcgaagtg gatgtcagcc
    11821 accagcggca cgttaacccg ctgtttgatg agtttgaacg cttctgccgc gtccatcgtc
    11881 ggtacggaga cacggacgat atcagcgcca acgcgttcca gcgccttgat ttgattgacc
    11941 gttgcttcga catctgtcgt acgcgtattg gtcatggact gtacggcgat gggagcacca
    12001 tcgccaatcg gcacattccc aacgtaaata cgtgttgatt ttctacgttg aattggagcc
    12061 tggttatgca tgaaaaatct cccgcgttac ccgtctgtta ctgcgccggt gattgttcgg
    12121 cattgagggt cagacgcgca acctggttag ttctgataaa acgactcaga tcgacaggtt
    12181 tcccttgata ctggatctgt actgcggctg gcgcaccaat tttcagttta tacggtgcct
    12241 ggccggttaa attcaaatta ccgtctttac gctgcatccc gctaaacaat tttttaccgg
    12301 tagcatcagt gacctccagc cagcaatcgg cagtaaagtt catcaccagc gcattcggat
    12361 cagccgccgg cgtggtcacg ccagcctgat cggttggcaa cggcgcagca ccatctggcg
    12421 ttgttgctgc cgttggtacc ggggtcgcgg cggtatcaac atttgcctgc gaaggcgaaa
    12481 caaccgcatt ctgttgcgga tcaacagctg gtgctggcgc agttacggca ggtgtttgcg
    12541 tgttggttgc ggtagtatcc acagacgctg gcggcgtgga cgttgtagcc gggtctgtag
    12601 ttgtcgacgt atttaacggg acactctgcc cctgctcgct attactgctc agttctgccg
    12661 aagattgatc ggccatagtg gtgatctctt cctgctgagc tttgtggtct tgccaccacc
    12721 aggcaccgct caggccgata accacaaaca acaccagcca ggtgaaggtc atcagccagc
    12781 catcacgttt tttgcggcgt ttaccgaggg aaaaactctg cattggcgca acttttgcag
    12841 cccgtagtgg agcctgcttt tccagccctg gcagcagttc ttcttctgga atatgtacca
    12901 gacgcgcata agagcggata tatccgcgca ggaatgttga agcaagatcg gcgggtgcct
    12961 tatcttcttc aatgtcgcgt accgtggaaa ccttcaggca aagtcgctcg gcaacggcct
    13021 gctgactaag tcctagttgt tcgcgagcat tacgcaggcg agcgccggta gtaagtgctt
    13081 cattttggtc gtgcgtggct tcagtattca ttcgctgcag gtacgtttaa atgagaattg
    13141 agatgccggt gaaccatcat gctcacccac accgcgaaac atccgttaag ttaaccgtta
    13201 tcatacagta taagaccgtc tgtgtgctcg tgacaaagcc cacattcact aaggctaact
    13261 tactgttgca tcgttacata ctgccttaaa gtcagcaaaa acgcaccgtt aatattgacc
    13321 agacaattgc aacttaatac ttcattcata aaacattacg ccacggtaca taaagtaacc
    13381 gtggcgtaat ggctatcaga ccgctttaat gtcgatggct tcaccctgca tccgtttacg
    13441 cagggtacgt ttcgtacggt cgataacatc gcccgccaac tgaccacagg cagcatcgat
    13501 atcatcacca cgagttttac gcacaatagt ggtgaaaccg tagctcatca gcacttttga
    13561 gaaacggtcg atacggctgt tcgagctgcg tccatacggc gcacccggga acgggttcca
    13621 cgggatcagg ttgatcttac acggcgtatc tttcagcagt tccgccagtt ggtgcgcgtg
    13681 ttcagtgccg tcgttaacgt ggtcaagcat cacgtattca atagtgactc ggccctgatt
    13741 ggcgttggat ttctccagat aacggcgcac cgcagcaagg aacgtttcga tattgtactt
    13801 tttgttgatc ggcacaattt cgtcacgaat ttcgtcgttc ggcgcgtgca gggaaattgc
    13861 cagtgcaacg tcgatcatat cgcccagttt atccagcgcc ggaactacac cggaagtaga
    13921 aagcgtgacg cgacgtttag acaggccaaa accgaaatca tcaagcatga tttccatcgc
    13981 cggaacgacg ttgttcaggt tgagcagcgg ctcgcccatg cccatcatca ctacgttagt
    14041 gattggccgc tgaccggtga cttttgctgc gccgacgatt ttcgccgccc gccacacctg
    14101 gccgataatt tccgacaccc gcaggttgcg gttaaagccc tgctgggcgg tggaacagaa
    14161 tttacactcc agcgcacacc ccacctgcga agagacgcag agcgtggcac ggtcgtcttc
    14221 cgggatatac accgtttcga cgcgctgatc gccaacggcg atcgcccatt taatggtgcc
    14281 gtcggatgaa cgctgttctt caaccacttc cggtgcgcgg atctccgcca cctctttcag
    14341 tttgccgcgc aacactttgt tgatgtcggt catctcatca aagttgtcgc agcaatagtg
    14401 atacatccac ttcatcacct gatcggcgcg gaagggtttt tcacctaaat ctttaaaaaa
    14461 ctcccgcatc tgctgacggt tgagatccag caggttgatt tttccatctt tcgtggtgac
    14521 gttttcaggt gtgactaatt gttcagacat atgctattcc ggcctcgtta ttacacgtta
    14581 tggcccctgg agggttgaaa aaagaaacgc cccggtgagc ggctgctcgt ccgggggcgc
    14641 tgcattgtac aaattctggc gcacggatgc cacgtttgca cgcggcattt acgaaattat
    14701 taacgggtgc gcgggcacac ttcgccttcg ccgaagaaat aagcgatttc gcgagcggca
    14761 gattcgacgg aatcagaacc gtgggtaccg ttttcggtca ggctgtcagc gtaatcagcg
    14821 cgcagagtac cggccagtgc gtttgccgga ttggtcgcgc ccagcagatc gcggtgacgc
    14881 tgaacggcgt tttcaccttc cagcacggaa accacgatcg ggccagaggt catgaattca
    14941 accagaccat caaagaacgg ttttccatcg tgttcagcat aaaagccacg agcctgttca
    15001 acggtcaggt gcagcatttt ggtgccgaca attttgaacc ctgcagcttc aaagcgcgca
    15061 aagatattac caatgacgtt ttttgctacc gcgttcggtt tgatgatgga aaaagtacgt
    15121 tcaatagcca tttttacctc tgtaaattgt tctgttgttg tctgtaccag cgtacgagat
    15181 ggcgcggatt ataatgagca acagggccgt tgactattga tgaaggtaac atttttttaa
    15241 aataaaatga gttttagcaa caatcaggga caaaacagac tattgcatga caaatttcac
    15301 tgtcgcgatt tgccccacgt catccatcac cagcaattga taatcacctt tatccgtcaa
    15361 atgcagggtc acgttgcgcc cgcgttcagt taacggttcg ccattcagaa accaccagcg
    15421 ttcacctgcc cctccacttg attgcaacgg caaagtcgct tccgctgcgc ccggtaaacg
    15481 tttaataatc gcgccatcgc gaacgcctgt cagctgcaga gggagctggg catcgtgacc
    15541 gtatggcggg caaattgtcg aggccggtgg taagcgcaca gcgcgacgtt ctgatgcggg
    15601 cagccagggt tccagcggta gcggccagac atttaccatt tcttgcttcg cctgcgggca
    15661 gtcagcggcg acgcgctgcc ctttgtcatc cagccagatg gggaagcgaa tgccattaat
    15721 cccttcctgc tctgacagta acagcgttgg cggctggctg ccatccagca gccaggttgc
    15781 caggcggcgg cgacagttac cgtcaccttc cggcaaagac tgtccgcccg gccagcagat
    15841 aacgccacga gtgactgagt tcgggcgcgg gtcttccggc agattcgcac tgcgcgacag
    15901 taagatatta ttgacctgat tcaacaatgg tacggcactg gcaaagccaa actgaccaac
    15961 aacgggcgtg ccgtcaggtc tgccagtcca gatcccaatg acatagcgag cgttaacccc
    16021 aatcgcccag gcgtcacgat agccatagct ggtgcccgtt ttccatgcca gtggggcgac
    16081 gcgcggcaag gcactatccg gcaagggttg cgcttcatca gccataatcc ggcgaatgat
    16141 ccacgccgcc cccgacgaca ttaaaggccg ttcaagcagc ggatcgtcag gctgtaagcg
    16201 caatttgcct gccttgccgt ggcgagcaaa cgcggtatac gctgccgcca tatcttccag
    16261 ttttgcacca gcgccgccga gaatgagtga aagattcggc gcagcgccgt tgggcaaata
    16321 taacggcaat ccaacattgc gtaactttgc cgcaaaccgt ttcggtccat aggcttccag
    16381 cacctgcaca gcaggtaagt tcagcgagcg caccagcgcc tcgctcatgc tgatcgggcc
    16441 atgaaaaccg ctatcaaagt tacctggtcg ataatcaccg gtgcgccggg ggacgtcttg
    16501 cagcagtgat gccgggtgga tcaagccttc atccagcgcc agaccataaa caaacggttt
    16561 gagcaccgat cctggcgatc ggatcgcatt gaccatatca acatgaccaa agcgtgaatc
    16621 atcgttgaga tcaaccgatc ccacccagcc gcgaacacgc atatcggtat gatcaaccac
    16681 gatcatcgcc agtgagctgc gcggtggcaa ccgccctttc cagttttgcg ccagttcttc
    16741 cagacgtcgt tgaagaccgg catccagcgt agtaacgatt ttgtcgcttt tgcttttacc
    16801 gagcatcatg cgcgaaaaca gcggtgccag ttgcggcatt tgtcggggtg ccagccagat
    16861 gggttcttcc cttgactctt ttacctgctc acgggaccac acaccttgcg cagccatccg
    16921 ttcgagcact ttattacgcg cggcttcggc acgctccggc caacgatccg ggcgaagacg
    16981 gctgggcgct tgcggcaaaa ccgccagcat tgccgcctcg gaatagctta aattcgcagg
    17041 cgactttccg agataagccc aacttgccgc accgatcccc tgcaacgtac cgccaaacgg
    17101 agcgcggtta agatacaagg tcagaatttc acgcttagac agatgccatt ccagttgcaa
    17161 cgcgcgccag agctggcgaa ttttgccgcc aaatgttttg gggtgaggat caagcagacg
    17221 agcaacctgc atagtgagcg tgctgccacc ggaaataacc cgtcccgaag tgagatcttg
    17281 ccatgctgcg cgcgccaccg agaatggatt cacccccgga tgcttccaga accagcgatc
    17341 ttcataattg atcagcgctt caaggtaacg tggagaaaca tcttcgattg ttaccggata
    17401 acgccagatg ccgtcagcat cggcgaagcg ccagagcggc gtaccatcct gcgccacgac
    17461 cactcgtgcg ggattgactt catgcagcgg tagaggccag agtttatctg ctccccacgc
    17521 tgcaagaaaa aggagaaatg gcgcagctgc cagcgttatc cagcagccgc gtttggttaa
    17581 caagcgaggc atttacggtc ggacaatcag cagatcttca gccgcgccgg tcgcccgcca
    17641 ttggggaaca tacattgatt ccaccatcgg ttgcggtacc tgatacgttc ccggcgtcac
    17701 cgcccgcgcc agatacacca gcgttaccgg ttggtattca tcaacggcaa ccgccgccac
    17761 aaagcgatcg tcacggaact caatgtgctt aatactcgcc tgctgcatct ggttcagtaa
    17821 gttctgcact tcgccaccac tttgctccag gctggcgcta ccgttcgcca gattctggtt
    17881 ttccagttcc agacccacag gcagcagatc cacgactaac gcatccggca cgctgttact
    17941 ggcttttacc tgcaaccaca ccagcaccag atcgccgcta cgtaacgagt ccagcgattt
    18001 gctcttacca tcagtaccaa gaatatgacg ctcgatttgc agcacattgt tcgccggtaa
    18061 aggtgcggat tgcggataac cgctggcatc catacgcaac cataacggct gatcgccact
    18121 gttgctcacc tgcaaggtga caagttgatc gctattcaga ttgctgtttt gcgctttctc
    18181 gcctgtcagc ggctcagctg agaaagaggt ttgcgcctgc catttacccg gtaaatcctg
    18241 aatcgtacgg gcagccagga acaacgcgtt actttcctgc gtcgatagcc agcgttcacc
    18301 aaacgcctgc tgcgaaagtg tgttcagcaa agtgtactgc tcatcgggta gcagtttatt
    18361 ttcttccagc aaggagagca ttaacgcgtt gtcgcgcagt ggactaccgt aatcacccag
    18421 ccatatccgc tcatcactat ttctcggcgt tttcagcgcc agcgcaatcg cttcttcacc
    18481 acgcgtcgca tcacccatgg ttttcagcgc aatgccaagt tgcagcagcg gtaaaccaga
    18541 agcggcatct gcacgatgct cccagatttc acgcagcgca cccagtggtg ctttttgctg
    18601 acgggccagc accagcgcag cgtaagactg aacggcgaat ttactggctt tgagattatc
    18661 cgcgtacggg atcgacatca tgcccggatc ttgtaaatag cgcagcagac gctcattacc
    18721 ccggttaatg gcgtctgtcg gcacgctgta accctgttcg cctgcgcgga ccaggaaatc
    18781 catcacgtaa gccgtcagcc agtactcttc gtcaccgttt ttatcccaca gcgcaaagcc
    18841 gccgttatca cgttgcattt gcagcaaacg ggaaatgccg atatcgaccg atgcacggcg
    18901 tttctcatca ctgtcgcctt tgatgcccaa cgcctgcagt tgggctgcgt tggtataaag
    18961 tgacgggaac aggccgctgg cggtttgctc aagacagccg tacggatacg cttttaactc
    19021 tttgatataa cgtgcaatgt tcagcggtgg tttgccgctc aacaacaatt gcccttccag
    19081 cgtaacaggc gagaagtttt gcaatccatc cgccggaatc gcccatgtct caccaggctg
    19141 taacgccgta ccgtaattaa ccgtttgtgc cgggaacgcc ggacggacgc cgattttcca
    19201 ctgcttatgc tgatcggcaa cggtttcacc cggtaacgct aacccgctaa tggtggcctg
    19261 aatttctcca tcgccataac ccggcaatgc tcgcaccggg ataaacagcg tagtacgcac
    19321 acctggcgct aattcaacgg ctgcgggtga atcgctgacc agttcaagca aaccactggc
    19381 ggtcagggca acgttcagtt tttgcggttt atcggtaaga ttagtgatat ccagcgtcag
    19441 acgcgaggta tcgccactcg ccataaagcg cggcatgttc agttcagcaa tcaccggtgc
    19501 ggcaactatc actttgcttt cgttgctacc gaaatcatct gccgtccagg cttgcgccat
    19561 gacgcgcaat tcaccgttaa aatcgccaat cggcagtgta accgagcctt cgccctgttc
    19621 gttgagcgtt accgacagcg cctgctgcgc gacaatattg acgtgattga ccggcggttt
    19681 accaccacgt ttcagctcat caccatcgcc accgaaacgc agagctgcca gacgcccctg
    19741 accttcaata acctgaccgt aaatatcgta aatgtctgcg ccatagcgtt tctgaccaaa
    19801 gaacgcctgc cacggatctg gcgtaacgta gtcagtaata ttcagcacac cactatcaac
    19861 ggcagacacc agcacattca cctgtttagg cttctcgcca tttttagtgc tggctttaat
    19921 tttcacggtt aatggttgat tgggacgcat ttttgctggt gtttccagcg ccagatcgag
    19981 gcgacggttt tcatcaccaa gcggcagatg caacacacca accgcgcgtt ttggcgtcgc
    20041 ggagcgagat ttatcgccag gacgcaccac cagcgtactc aaataaagat catgacgatt
    20101 ccaggtttta tcgaccggaa tcgtcagatc cagcccttga gccggaacat caatctcttg
    20161 ccaccacagc ggcccttcac tggactcgac catcgcataa cctttacccg ccgttggcgc
    20221 ggcaatatgc aacttaatgg tatcgccggg gcgataactg gctttatcta gtttcagcgt
    20281 gacacggtcg ggtcgcactg cgccgctccc gtcgctgttg tcctgccagc tatagccagc
    20341 ccaaaaacgc acgctactga ccgcttcatt cggcgctttg acttccagac gataagcgcc
    20401 ccactctacc gggaaactga ccttaccggt ttcatccgcc tgaagatcca gagtttgttc
    20461 attttcgatc agatcttttt gatcaaactg tgactgccag ccttcatctt ctgaccagtt
    20521 ccagtagtaa tcgcggcgtt cgcgaatcag gcgcacctgc aagcccgaca cggctttttt
    20581 cacgccttgc gcatcgctat aaacgatgtc aaaagcggcg ttactgcctt catcaacaat
    20641 gggctgtttc accgtgctgt cagtgcgata atcgtaaacc gatttcaagg cgaactgcgg
    20701 acggagcccc ggcaatgcat cggcaggcca gatagcctgc tcagcgcggc gcgtcaccgg
    20761 gcgaccgccc gattccagca aactgccctg gaaaataacc tgcaatgggg aatgcgtttc
    20821 cttccactgg ctttctgtag aaacttcgcc gcgcccttta tcatccagcg tcaactgaac
    20881 ttcatccagc gtgcgggaaa ggttttcggc agctatatcg ccgaattcaa aaccaggtaa
    20941 ggctgacaca gcctcacgca gtgggcgcag gaaaagttgc ccttgcaaag tattaccatt
    21001 agcaggcgca ccgtacaggt agtatcccac cacggagaat ttcacttcat cattcggcgt
    21061 aagcggggtt ttctcaccgg tcagattcag cgccatacgc tctggcataa aatcttcgac
    21121 gtggaaatcc cacatccggt actggttatc gcccgtgttg gcgcgaatat gccacatacc
    21181 ggttgccgca ttgctatcga gtggccaggt aaagtggtag aggccattct ccggctgact
    21241 aacgacgttc ctgagtacct gcccgtcagg tttaatcacg tctaacttga tgggttgatc
    21301 gggcaatgct ttaccgtctg catcgcgcag taaaccattg aggattaccg tttcacctgg
    21361 gcgataaaga tcgcgtgggc caaacatgaa aaactgtttg ctatagcctg gcgcgccagc
    21421 aatgttaaat tctgctaagt ccagcgccgg aagttttaaa tcgagtagcg ttgtctgacc
    21481 gtctttacgt gccagcagta atgccgcatt tttatcattt tccagctgca catgcccctg
    21541 agcgtcactg gttgcctgag tcagagtctg tcctttctca tttaataaag agacttcaat
    21601 tccttgctgg gccgcgccgt tttccagact ttgggtaaag atatccagac aattgtgata
    21661 acggtgagct gaaacgccga tatcacttaa cgtaaacagc gtcgcgggat tactgtaatc
    21721 gtaacgtcca gcctgattca tcacagccag atacacgccc gcctgctgaa gcggtttgat
    21781 atcgcccagc ggcagcaata atttttcacg ggtgttacgc gcaggattga gatcaaaccg
    21841 tccggtgtag accagatccg ccatctgcag cagtttgtct gactgccagt tcgccagcga
    21901 attgcggtat tcccattggc taatgaatgc tggcagagat tctggcttaa cgcggaagaa
    21961 gttaacatcg acattattaa cgttgagcgc cattaccggc agcccttcaa cgactttgcc
    22021 aggcagcagc gaaccacggc tggcaaaacc gacgctgggt tggatgtcgc gggtagttat
    22081 agttttttcg taatctttac tgaaggttgc gttgttgagt gctttgacct ccttgccaat
    22141 agtaacgatc aaatcacgtt tcggttcgag gtggcgtaaa cgtagctctt taagattatc
    22201 tgacagctcc caggcaccat ccactttgcc gcttttttta tcgacgacat gaataacgcg
    22261 tgagaaatcc tgatccgggt cgagagggat ggagaacgtc agcaccagcg tggctgcacc
    22321 atcaagttgg acttcagaga gatccagcaa tgtcagcacc ttaccggcac tctgttgggc
    22381 cagtttttgt ctctccggca cggagagttt tgctgaactc gcgttttctg aagaggccgc
    22441 tttagtaact tcagaaggcg catctttttt caccgctgtt ggcgcgttat cgttgttgtc
    22501 gcaccctgcc agcgccagca ttagcatgca ggcggctacg cgtaactttt tcatttttca
    22561 tccctgccac aatggcccgt tagcaacgtc gaataattat tatgcgtgag aatttacgtt
    22621 atgtaattca gtatcacgcc catacaatcc gaattttagt gagaattgtg gctcagacag
    22681 ccaaatcagc ctctttcgct tgtcgctttt cactaaacat ccgacaattt agcctaaccc
    22741 cggcaaaaat ggagatgcct atgtccacga catggtttgt aggagccgac tggctcgccg
    22801 aacatattga tgacccggaa attcagatta tcgatgcccg catggcgtcg cctggacagg
    22861 aagatcgtaa cgttgctcag gagtatctga atggacatat tcccggcgca gtgttttttg
    22921 atatcgaagc gctttctgat cacacttccc cgcttccgca catgctgccg cgcccggaaa
    22981 cgttcgccgt ggcgatgcgt gaattaggcg ttaaccagga taagcacctg attgtctatg
    23041 acgaaggtaa tcttttctca gccccacgag catggtggat gctgcgcacc tttggtgtag
    23101 agaaagtgtc gattctgggg ggtggacttg caggctggca gcgcgatgat ctgctgttag
    23161 aagaaggtgc agtagagctg ccggaaggag agtttaacgc cgcgtttaat cctgaagccg
    23221 tggtgaaagt aaccgatgta ttattggcaa gccatgaaaa tacggcgcaa attattgatg
    23281 cccgcccggc tgcacgtttt aacgcagaag ttgatgaacc tcgcccaggt ttacgtcgcg
    23341 gacatattcc cggagcactg aatgttccgt ggacggaact ggtgcgcgaa ggcgaactaa
    23401 aaacgaccga tgaactggat gcgatatttt ttggtcgcgg cgtcagctac gacaaaccaa
    23461 ttatcgtcag ctgcggctct ggtgtaacgg cagccgtggt tttgttagca ctcgcgacgc
    23521 tggatgtgcc aaacgtgaaa ctgtacgacg gcgcatggag tgaatggggc gcgcgggcag
    23581 atttaccggt tgagccagtg aaataagtat tttacaggca ataaaaaacc gccgaatttg
    23641 gcggtttttt attgctggtc tggttcgcgg cctttccagc aggttgtatt accgtagtaa
    23701 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
    23761 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
    23821 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
    23881 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
    23941 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
    24001 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
    24061 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
    24121 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcacaatatg ttcggtaata
    24181 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
    24241 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
    24301 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
    24361 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
    24421 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
    24481 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
    24541 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
    24601 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct
    24661 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
    24721 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
    24781 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
    24841 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
    24901 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
    24961 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
    25021 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
    25081 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
    25141 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
    25201 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
    25261 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagcaa acgaccggca
    25321 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
    25381 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
    25441 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
    25501 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
    25561 cagcagagga acagcttcac gcgcttgttc agtccgcgcg taatggcaaa tgccagcgcc
    25621 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
    25681 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacact
    25741 ggcgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
    25801 gaaccgcgtc cgacggtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
    25861 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
    25921 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
    25981 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
    26041 actttacgcg tgcctttcgg ggctttgtaa ccttgccaga atgcccagca gcgatccgca
    26101 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgcgcc
    26161 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
    26221 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
    26281 atcttcatcg cttctgtcat tttagttatc cttctttgta aaagggccgc cagttggcag
    26341 ccccaggatt tttccgctaa atgattcgcg ttgcagaaag gcggcaagtg agtgaagccc
    26401 caggagcata gataactatg tgactggggt gaacgagcgc agccaacgca tctgcggcgt
    26461 gaagcatgac gcggaaatta ttcggcctcg tccagccaga ctaacaaaat cgcttcgaga
    26521 attttctcgt tggatgcctg cgggtcgtcg tcgaaatctt ccagatcgca aatccactga
    26581 tgcatatcgg tgaatcgaac cgttttcgga tcaagatcag gatacgcatc gtacagtgct
    26641 tcgccaattt cgcggctatc ggtccactta agtcccatac taacctctgt taatgctcac
    26701 gcgcatggtt gatagtgtaa cgcgggattt cgactactaa atcttcgtcg gtaacacgtg
    26761 cctggcagct taaacggctt tcgggctcca gtccccaggc tttgtccagc atgtcgtctt
    26821 cctgctctga gctttctggc agcgagtcaa aaccttcacg aacgatgcag tggcaggtgg
    26881 tgcaagcaca ggatttttca caggcgtgtt caatctcgat accgttacgc agagctgcgt
    26941 cgagaatggt ttcaccgcta ttagcttcca gaacagcgcc atcaggacag agatcctgat
    27001 gaggcaaaat aacaatcttt ggcatattaa acctcgtcca cggaatggcc tttcagcgca
    27061 cgacgaaccg actggtccat gcggcgagcg gcgaaatcct gggtttgttt gtctacgttt
    27121 ttaatcgctt gttcgatggc gtcaacgtca tcgccctgcg ccacttcact caggtgagcg
    27181 gcagcatcgt caatgacctg acgttctgcg gcgcttaaca gcgcggcatc agcagccagc
    27241 gcgccgtgca gactttccag cacacgcgcc gcttctactt tttgttctgc cagcattcgg
    27301 gcttttacgt cctgctcggc atagctcatt gagtctttga tcatcgaagc gatttcgcta
    27361 tcggtcagac cgtaagacgg tttgacctga atagacgcct caacgccggt ggatttctcc
    27421 atcgccgtca cgctcaaaag accgtcggca tcgacctgga acgtcacgcg aatatgcgca
    27481 ccaccagccg gtagcgccgg aataccacgc agcgcaaaac gcgccagtga gcggcagtcc
    27541 tgcaccagtt cgcgctcacc ctgcattaca tggatagaca tcgccgtctg accatcttta
    27601 aaggtggtga aatcctgagc gcgggccacc ggaatagtgg tattacgcgg aatcactttc
    27661 tccaccaggc cgcccatcgt ttcgaggccc agcgacagtg ggatcacatc aagcagcagc
    27721 atttcgctgt ctggcttgtt acccaccaga atatccgcct gaatcgccgc gccaatagcg
    27781 acgactttat ccgggtcgat ggaagtcagc ggtggacgac cgaaaaattc gcctacccgt
    27841 tcacgcacca gcggcacgcg agtagaaccg cccaccatca ccacttccag cacttcgtca
    27901 gcttctacac ccgcgtcttt aagcgcgcga cgacaagcca gtaaggttcg ttttaccagt
    27961 ggcgcgatca gttcattgaa ttgttcacgg ctgatttcgc cctgccagcc cgcaacgtta
    28021 acggtcacgg agtccgcatc gctcagcgcg attttggctg caatggcggc atccagcagt
    28081 tcacgctgaa cgcggttatc gctacgatca ggaatgcccg cctgctcgcg aatgtaatcc
    28141 gccagcagat ggtcgaaatc atcgccgccg agcgcggaat caccgccggt tgccagcact
    28201 tcaaacacgc cgcgacttaa gcgcagaatg gaaatatcaa acgtcccgcc accgaggtca
    28261 taaacagcga tcacgccttc ctgaccggaa tccagcccgt aggcgatagc cgcagcggtc
    28321 ggttcgttaa gtaagcgcag aacatgcaac cccgccagac gcgccgcgtc tttggtgccc
    28381 tgacgctggg catcgtcaaa gtacgccgga acggtgataa ctacaccatc cagctcgcct
    28441 gccagggctt cagttgcccg cgccgccagt gctttgagga tgtccgcaga aacgcgcacc
    28501 gggttcagca gccccgccgc cgtttcaatc atcggcaggc cgttttcgct ggcctggaat
    28561 tgataaggca gatgcggata gcgttgctgg atatcagcca gcgagcgccc catcaggcgt
    28621 ttaacagaac taatggtgtt ggcggtgtca agcgctgcgt tagtacgcgc gtcataaccc
    28681 accgaatgcc cttgctgttg atagtgaaca acagatggca gcaggtgacg gccttcatgg
    28741 tcggctaacg tttcggcctg accgctgcgc actgtcgcca ccagcgagtt ggttgtgccc
    28801 aggtcaatac cagccgccag acgacgctga tgcggcgcag cactcaaacc aggttcacta
    28861 atttgtaata aggccatgtt tagcttccag aaattaaaaa tcgagcagtt tttcttcgag
    28921 ttgttcggca ctgctccgca gtttatcgag aaaacgcagc ttacgcacgg tatccgccgc
    28981 cgcgtcccac gcctcgttgt ctaactgttc aaccatcaac tgatggcggg tatcaaacat
    29041 ctttttcaca cgtttgataa agctttccag ccgcgcttca tctttcgcct gttcgatctc
    29101 gtccagctct tcgcgcagct ccaactgttc catcaggaac gcggtgtcgc gcacagtatg
    29161 ctgctcgctg gcgagatcaa aaccgtgtaa agaaagcaaa tattccgcgc gcattaacgg
    29221 atgacgcagc gtttgccagg cctggttaat ggttgcagat tgctgtacgg cggcgagttg
    29281 ttccgcctgg cttccgctgg cgaatttatc aggatgatac tgacgttgta gatcctgaaa
    29341 acgcaggctc agcgcctggg tatcgagttg ataacgggca ggcaagccaa agaaggtgaa
    29401 gtaatccata acattctcag ggctgcgatc cgccgcgata acggcgaatc aggtaaaaca
    29461 aaccccacgc gcaggcgacc acggtggggt tatcggtatg cgcatcaaac gtggaagctt
    29521 tcgccgcaac cacactcatc tttgacgttc gggttggtga atttaaaccc ttcgttcagg
    29581 ccttctttta cgaagtccag ctgcgtaccg tccagaaatt gcaggctttt gccatcgacc
    29641 acgactttca cgcctttgtc ttcaaacacg atgtcttccg gcgtcggttc gtcaacaaat
    29701 tccagtacat aagccatacc tgaacatccg gaggttctca cgcccagacg caggccaaac
    29761 cctttgccgc ggttagccag aaaggtattt actcgcgctg ctgcactgtc gctcagtgta
    29821 atcgacataa ccaaacctca actcttattt tgcttcacgt ttgcttttat agtccgcaat
    29881 ggcggctttg atcgcgtctt ctgccagaat agaacagtga attttcaccg gcggcagttc
    29941 aagttcttca gcaatatcgg tatttttgat cgcctgcgct tcgtcgagag acttcccttt
    30001 cacccattcg gtgaccaggg agctggaagc gatagcagaa ccgcagccgt aagttttaaa
    30061 acgcgcgtct tcaatgatac cttcatcgtt gactttaatc tgcaacttca tcacgtcgcc
    30121 acaggccggt gcccccacca tgccgctgcc gacgttctcg tcgttgttgt caaaggaacc
    30181 cacgttacgc ggattctcgt aatggtcgat aactttttcg ctgtaagcca ttataaattc
    30241 tcctgattcc gataccgatt aatgatgagc ccattcgatg ctgttcagat ccacgccctg
    30301 cttgtacatt tcccacagcg gagaaaggtc acgcagacga ccgatggatt tacgaactaa
    30361 ctcgatggtg tagtcgatct cttcttcagt agtaaaacga cctaaagaga aacggataga
    30421 gctatgtgcc agctcgtcgt tcagccccag cgcgcgcagc acgtaggacg gttcgaggct
    30481 tgctgacgta caggcggaac ctgaagaaac tgcgaggtct ttcagcgcca taatcagcga
    30541 ctcaccttca acgtagttga agctgacgtt gagaatgttc ggcgcaccgt gttccaggtc
    30601 accgttcagg taaacttctt cgatatcttt gatgccgttc cacagacggt tacgcaggcc
    30661 gcgcagacgt tccatctcgg tcgccatctc ttcttttgcg atgcgatagg cctcgcccat
    30721 tccgacgatc tggtgaacag gcagagtgcc ggaacgcata ccgcgctcgt gaccgccgcc
    30781 gtgcatttgc gcttcgatgc gtacgcgcgg tttacgacgt acatacagcg caccgatacc
    30841 tttcgggcca tagattttgt gaccggagaa agacatcagg tcaactttca actggctcag
    30901 gtcgataggc agtttgccca cgctctgggt tgcatcaacg tgatagataa tgccacgagc
    30961 acggcacatt tcgccgatag ccgcgatatc ctgcaccacg ccgatttcgt tatttacgtg
    31021 catgatggaa acaaggatgg tgtcatcacg catcgctgct tcaagttctt tcagatcgat
    31081 aatgccgtta cgctgcggtg ccaggtaggt gacttcaaaa ccttcgcgct ccagctgacg
    31141 gcaggtatcc agtaccgctt tgtgttcggt tttgctggtg atgatgtgct tgcctttttt
    31201 ctgataaaag ttggctgcac ctttgatcgc caggttgtca gattcggttg caccagaggt
    31261 aaagacgatt tcacgcggat cagcgccgac cagatcggca atctgattac gggcgatatc
    31321 taccgcttct tcagcctgcc agccgaaacg gtgagaacgg gaggccgggt taccaaaggt
    31381 tccgtccatc gtcataaact gcatcatttt ctcggcaaca cgcgggtcca ccggcgtggt
    31441 tgcggagtag tcgagataaa tcggtaattt cattgctcta taaactccgt acatcactca
    31501 atgcaaggaa ccaggctacc ggctggatgt acgaccgtgt ttacggagcg cgatacgccc
    31561 cggcctgatt ctaatatctt gttatcttta acctaactgt ttcgggtttc agcaagttga
    31621 caaacttacg aatctccggg agcttacata agtaagtgac cggagtgagt atgtgcagtc
    31681 aacgcagatg caacacgaaa caggacggtt aaattaagcg cgtaacttaa cgtcgatcgc
    31741 gtcttgtatg cgggtgcgtg gcgcgtcgtg agtatgctga cgaccagaca catccagcac
    31801 ttcctggtta ttaaccagtt cgcctaaagt aatgttgttg agaaaaccgg tgagacggtc
    31861 gctcaaatca cgccacagcg cgtgggtcag gcatttatcg ccgccctggc aaccgccttt
    31921 accctgacaa cgggtagcat ctacagattc gtcaacggcg ctaatcactt cgccaacggc
    31981 gatgctgctg gcatctttgc ctaacagata accaccgcct ggtccacgta cgctggaaac
    32041 cagaccattt ttacgcagac gggaaaacag ttgttccaga taagaaaggg aaattccctg
    32101 acgttcggaa atatcagcca acggtaccgg gcccgcttca gagttgagcg caacgtcaag
    32161 cattgcggtc acggcatagc gccctttaga tgtcagtctc atgtcttact tcacctcaaa
    32221 ctcgcccctg cccggggttt tttattgtaa aagtgggggt attgcatagc agggtcaagt
    32281 ctgacattcc cgagtaaatt ggtcaactat ttacttgact gatttagtcg ggtatttaac
    32341 cttcagtgcc atttttttat cgtggcgttt gtacctgttg tccttccaga ccatgggcgg
    32401 gttgagggca attattcgca ttagcgctgg cctcttcccc tcaccctaac cctctccccg
    32461 aaggggcgag gggaccgtcc gtgcatttat ctggcttagc cctcaggcct tattaccttt
    32521 attctgctgc tctatagaag ccagaatccc gcgcaggata ttcaattcct ggctttccgg
    32581 gcgcgcacgg gtaaacagac ggcgcaattt attcatcacc tgccccggat ggttttcacg
    32641 gataaaaccg gttgccagca gcgtttgttc cagatgacca taaaaacgct ccagatcatc
    32701 gaccagcgga tacggcgtct cttcatgctc gacctgctcg ccattttcct gagttgccag
    32761 ccaggccata cgcacttcat aggcaataac ctgaaccgcc attgccaagt tcagcgaact
    32821 gtattccggg tttgccgcaa tcgcgacatg ataatggcat ttctgcaact cttcattggt
    32881 caagccgacg cgctcacgac caaacaccag cgccaccggg gtatttgccg cttcagcgac
    32941 gcttttcaga ccgcattcgc gcgggtcgag catcggccac ggcagcgtgc gggagcgtgc
    33001 gctggtgccc accaccaggc tacaaccggc taacgcttcg tcgagcgtat cgacgatgtg
    33061 agcattacca atcacatcgc tggcccctgc tgccagggca atcgcctggg agtcgggttt
    33121 caccagtgga ttaaccagcc acagattggt taatcccatt gttttcatgg cacgggcaac
    33181 agaacccata ttgccggtgt gtgacgtctc caccagcaca attcgaatat tttgcagcat
    33241 tgtctttctt catctaaaga ttattcacgc atcttatcat aaaacgaaga cagatgccga
    33301 tctcgctgct atactctgcg ccgttttccc gttctttaac atccagtgag agagaccgat
    33361 gcatccgatg ctgaacatcg ccgtgcgcgc agcgcgcaag gcgggtaatt taattgccaa
    33421 aaactatgaa accccggacg ctgtagaagc gagccagaaa ggcagtaacg atttcgtgac
    33481 caacgtagat aaagctgccg aagcggtgat tatcgacacg attcgtaaat cttactcaca
    33541 gcacaccatc atcaccgaag aaagcggtga acttgaaggt actgatcagg atgttcaatg
    33601 ggttatcgat ccactggatg gcactaccaa ctttatcaaa cgtctgccgc acttcgcggt
    33661 atctatcgcc gttcgtatca aaggccgcac cgaagttgct gtggtatacg atcctatgcg
    33721 taacgaactg ttcaccgcca ctcgcggtca gggcgcacag ctgaacggct accgtctgcg
    33781 cggtagcacc gctcgcgatc tcgacggtac cattctggcg accggcttcc cgttcaaagc
    33841 aaaacagtac gccactacct acatcaacat cgtcggcaaa ctgttcaacg aatgtgcaga
    33901 cttccgtcgt accggttctg cggcgctgga tctggcttac gtcgctgcgg gtcgtgttga
    33961 cggtttcttt gaaatcggtc tgcgtccgtg ggatttcgcg gcaggcgagc tgctggttcg
    34021 tgaagcaggc ggcatcgtca gcgacttcac cggtggtcat aactacatgc tgaccggtaa
    34081 catcgttgct ggtaacccgc gcgttgtgaa agccatgctg gcgaacatgc gtgacgagtt
    34141 aagcgacgct ctgaagcgtt aatgactcag gcgggtgatg tcactcaccc gccctcgcct
    34201 ttcaggcgct attctgaaat acttcctcac cgctttactt tctttcgtca ctctcccacc
    34261 atctttcccc gattaatgga tgataaagaa gtcacaagga aaggttatgg cgctgccagt
    34321 gaacaaacgc gttctcaaaa ttctgtttat tctctttgtt gttgccttct gcgtttattt
    34381 agtgccgcgc gttgccatca acttcttcta ttatcctgac gataaaattt acggtcccga
    34441 cccctggtcg gcggaatccg tcgaatttac ggctaaggac ggtactcgtc tgcaaggctg
    34501 gtttattcct tcttcgacgg gccctgctga caatgccatc gcaaccatca ttcatgctca
    34561 cggcaatgcc ggaaatatgt ccgcccactg gccgctggtc agttggttac ccgagcgtaa
    34621 tttcaacgtt tttatgtttg attatcgcgg gtttggtaaa tcaaaaggca cgccgtccca
    34681 ggccggattg ctggacgata cgcaaagtgc catcaatgtg gtgcgccatc gcagtgatgt
    34741 aaacccacaa cggctggtgc tgttcgggca gagtattggc ggggcgaata ttcttgctgt
    34801 tattggtcag gatgatcgtg aaggcatacg tgcggtgatc ctcgactcca catttgcctc
    34861 ttatgcaacc atcgccaatc agatgatccc cggcagtggc tacttacttg atgagagtta
    34921 cagcggcgaa aattatatcg ccagcgtcag cccgatcccg cttttactca ttcacggtaa
    34981 agctgatcac gttatcccat ggcagcacag cgaaaagttg tatagcctgg caaaagagac
    35041 aaaacggttg atcctaatcc cggatggcga acacattgat gctttttccg atcgtcacgg
    35101 cgatgtttat cgcgaacaga tggtggactt tatccttagc gcgttgaatc cgcagaacta
    35161 acccatgatc gctagcacga taatcattca caaaaccacc ttaagacatg ctaatccact
    35221 ggtcagaaca gtttaagatg agaaaaattc tgtgacgctt gccaacattt ctgatgatta
    35281 gcattccctt cgccatttcc ctgagcaaac tttagctatt cttatcaatt atgcttatgg
    35341 gagatctaca gatgatgcct acgcttgctc caccatctgt cctttcggct ccccagcgcc
    35401 gctgccagat cttgctgacg ctctttcagc cggggttgac cgccaccatg gcaaccttca
    35461 gcgagcttaa tggtgtggat gatgatattg ccagtcttga tatcagcgaa acaggacggg
    35521 agatcctgcg ctatcatcaa ctcacactga cgactggtta tgacggtagc taccgggttg
    35581 aaggtacagt gcttaaccaa cgtttgtgtt tatttcactg gctacgacgt ggtttccgtc
    35641 tgtgtccgtc atttattacc agccaattca cccccgccct gaagagtgaa ctgaagcggc
    35701 gcggaattgc gcgtaacttt tacgacgata ccaatctaca agcgttagtg aatctctgct
    35761 cccgacggct gcaaaaacgc tttgaaacgc gcgatattca tttcctgtgt ctgtatctgc
    35821 aatattgttt gctgcaacac cacgctggaa ttacgcctca gtttaatccg ctccaacgtc
    35881 gctgggcgga atcctgcctt gaatttcagg tagcgcagga aattggacgc cactggcagc
    35941 gtcgggcgct ccagcctgta ccgcctgatg agccactgtt tatggcatta cttttttcca
    36001 tgttgcgggt tcccgatcca ttgcgtgatg cgcatcagcg agacagacaa ttgcgtcagt
    36061 ctatcaaacg tctggtaaac cattttcgtg agctgggaaa tgttcgtttt tatgatgaac
    36121 aggggttatg cgatcagctt tatacccacc tcgcccaggc gttaaatcgc agtttgtttg
    36181 ccatcggtat tgataatacc ctgccggaag agttcgccag actgtaccca cgcctggtgc
    36241 gcaccacccg cgcggcgctg gccggatttg aaagtgaata cggcgtccat ctttctgatg
    36301 aggaaagcgg tctggtcgcg gtgattttcg gtgcctggct aatgcaggaa aacgatctgc
    36361 atgaaaaaca gattattcta ctgaccggga atgatagcga gcgagaagcg cagattgagc
    36421 agcagttacg cgaactaacg ttactgccgc tcaacattaa gcatatgtcg gtaaaggcat
    36481 ttttgcagac aggcgctccg cgtggcgcgg cactgattat tgcgccttat accatgccat
    36541 taccgctctt ttcaccaccg ctgatctata cggacctgac gctgacaaca catcaacagg
    36601 agcagatccg caaaatgctc gaatcagcat gagggaacaa ctttcgggcg caaaaacatt
    36661 gccggaagcg ccaccagcgc cattacccag aacacgccgt ggcccagata ttgatacagg
    36721 aaaccggcga aaacggtcat gatagcgata ctgccgccca tcgcgacggc agagtaaacc
    36781 gcctgtaaac ggatgacttc gctaccctgg cgaacagcaa tatagcgcat ggcggccagg
    36841 tggcagaccg tgaaggtgcc gcaatgcaaa atttgcacca ctatcaacca cggcaacgcc
    36901 gtagttgctc ccataatgcc ccagcgcact acgccgcaaa tcgccgagat caacagcata
    36961 tcgcgagcac tacaacggcg gaaaagttta ttactcagcg caaagataat gacttccgcc
    37021 accacgccca gcgaccacaa ataccccacc gccgaggccg agtagccagc tgcctgccag
    37081 taaatggcgc taaaaccgta ataggccgca tgtgccccct gcaataaaca aacgcaggcc
    37141 agaaagcgcc agttctggcg aaccagcgcc aaccacgcag accaaccggt gctctcctgc
    37201 tggcgacttg ccccttgtgg ctgaatcgtc ggacggatga gaaagccgag cagcatggat
    37261 gccacgccca acgtcaacag cgcgaggatc acccgataat caaacatagt gaccagtttg
    37321 cccgtcagcg ccgagccaat gacaaacgcc accgagcccc acagtcgcac tttgccgtaa
    37381 tcaagcggga actgcttttg ccacgtattc gccagtgcat cggtcaacgg taccagcggt
    37441 gagaaaaaga ggttaaagcc aatcatcacc agcatcagcc acgctacgtg cgcccccgcc
    37501 cagaaggcga cagcaaagag aagtgtcagc agtgccagca cgcgcaaggc ggaaatcagg
    37561 cgggaaggat cgctgacgcg gggcgcgatg agcaaactcc cgagaaaacg ggcaaccaga
    37621 cctgccccca ataacaggcc gatggtttct ggcgttaaac caatcccttt aagccagacg
    37681 ctccagaaag gtagaaaaat gccgtaacta aaaaagtatg tgaaatagcc gagcgccaac
    37741 cagcgcgtgg attgcaaaac catgagtccc tcccgtcagg aggcgttagt ctggggttaa
    37801 tcgaatgttg gtgcaagttg aaaaataaga atattcgagc ctgcttcccg atgtctgtaa
    37861 cgccagttca caagcactaa aaaactgcga tccgcgacgc atgttgcaaa aataatgcgt
    37921 aattatgccg ttacgcttgc caaacgttcc tgcacaatgg cgatgaaatc gcgtaatgcc
    37981 ggcttcatct cccctttctt ccacgccatc agtaaagcaa tggaaggcac atttccggca
    38041 atgggacgaa aaacaacctg cccggtatta aaattattca tataaccggg tatcaaagtg
    38101 acgcccagcc ccatgcccac cagattcatg gtcaccagaa tattcgttgc cacctggacg
    38161 atatttggct ggctgttttc ttgcgcaaac cacgctttaa cgatcggcgc aagcgaaccg
    38221 gaatacgccg gatcggtact gacgaaattc acgccatcca gttgcgcggc ggtgatctct
    38281 ttttcatgtg ctaaagggtg atcaaccggt aacaccacca ctaatggttc gtcaaaaagc
    38341 tccagataat caatctccgg gctataaaca ggatggcgca ttaagccgac gtcgagttca
    38401 ccacggcgaa ttttttcctc ctgttgcgtg gtgattaaac tcaccagctc aatcaaggtg
    38461 tctggctgtc tgagacgaaa cattggtaat acttttggca ataaattcac ttccgccgat
    38521 ggcacaaagc caatggttaa ttgtctgtct tcctgaacaa ttttccgcgc ccgtaatttg
    38581 gcattttccg cttgttcgag gattgccagc gcgtcctgga gaaaacattc tcccgccgcc
    38641 gtcaacgcga ctttgcgctt atccctcacc agtaacggaa caccgacaca gttttcaaga
    38701 tcgcggatct ggctgcttaa cgaaggctgt gaggtatgca gtttttccgc cgcacgggta
    38761 aagttcagtg cctgcgccac tgcgacgaaa tagcgtaaat gccgtagttc catcaccttc
    38821 cccttgttat cgaaaaaacg tctcagctgg tagaaattaa ctatttcaca tattagcaac
    38881 caaccaacaa catccttatg acacaaaaat agaaggtcaa tacatcttat ctttcaggat
    38941 taaaaaatat gaccacaccc tcagatttaa acatttacca actgattgat acccaaaatg
    39001 gtcgggtcac tccgcgtatt tataccgacc cagatattta ccaactggag cttgaacgta
    39061 ttttcggccg ttgctggtta tttctcgccc acgaaagcca gatcccaaaa cctggtgatt
    39121 tctttaacac ctacatgggg gaagatgcgg ttgtcgtagt gcgtcagaaa gacggcagca
    39181 ttaaggcgtt tctcaaccaa tgccgccacc gggccatgcg tgtgagttat gcagattgcg
    39241 gcaacactcg cgcctttacc tgtccgtatc acggctggtc ttatggcatt aacggcgagt
    39301 tgatcgatgt accgctggaa cctcgcgcct acccacaagg gttgtgtaaa tcccactggg
    39361 gactaaacga agttccttgt gtggagagtt ataaagggct gatttttggc aactgggata
    39421 ccagcgcacc gggcctgcgt gattacctgg gtgacattgc ctggtatctg gatggcatgc
    39481 tggatcgtcg cgaaggcggc accgaaattg tcggcggcgt acaaaagtgg gtgatcaact
    39541 gtaactggaa attcccggca gagcagttcg ccagtgacca gtatcatgct ctgttcagcc
    39601 atgcttctgc cgttcaggta ttaggggcga aagatgatgg cagcgataag cgcctcggtg
    39661 atggacaaac cgcccgcccg gtgtgggaaa ccgccaaaga tgcgctgcaa tttggtcagg
    39721 acggtcacgg tagcggtttc ttctttactg aaaaaccgga tgctaatgtc tgggtcgatg
    39781 gcgcagtttc aagctattac cgcgaaacct atgccgaagc agaacaacgt ttaggtgaag
    39841 ttcgcgccct gcgcctggcg ggtcataaca atattttccc cacgctttca tggctcaacg
    39901 gcactgccac gctccgcgtc tggcatccgc gcggccctga tcaagttgaa gtgtgggcgt
    39961 tctgtattac tgacaaagcc gcctccgatg aagttaaagc cgcttttgaa aacagcgcca
    40021 ctcgtgcttt tggtcctgct ggttttctcg agcaggatga ctcggagaac tggtgtgaaa
    40081 tccagaaatt gcttaaaggc caccgcgccc gcaacagcaa actgtgtctg gaaatggggc
    40141 ttggtcagga aaagcgccgc gacgacggca ttcctggcat tactaactat atcttttcag
    40201 aaactgccgc tcgtggaatg taccaacgct gggccgatct tctgagtagc gaaagctggc
    40261 aggaagtgct cgataaaacc gccgcttacc agcaggaggt gatgaaatga gtgcgcaagt
    40321 ttcactagag ttacatcacc gcattagcca gtttctcttt cacgaagcca gcttactgga
    40381 cgactggaaa tttcgtgact ggctggagca gctcgacaaa gagattcgtt acaccatgcg
    40441 caccacagtt aacgcgcaaa cacgcgaccg ccgcaaaggc gtccagccac cgacaacctg
    40501 gatttttaat gacaccaaag accagctgga gcggcgaatc gcccgtctgg aaacgggcat
    40561 ggcctgggca gaagagccgc cgtcacgcac ccgtcactta atcagcaact gccagataag
    40621 cgaaaccgac atcccaaacg tatttgctgt gcgggtaaat tatctgcttt atcgggcaca
    40681 aaaagagcgc gatgaaacat tctatgttgg aacgcgtttc gacaaagttc gccgtctgga
    40741 agatgacaac tggcgcttgc tggaacggga tatcgtcctg gatcaagcgg taatcacttc
    40801 ccataacctg agtgtactgt tctgatgaat cgaatttatg cgtgtcccgt tgcggatgtg
    40861 ccggagggtg aggctctccg gatcgatacc tcgcccgtca tcgccctgtt caacgttggc
    40921 ggcgagtttt atgccattaa cgatcgttgc agccatggta atgcgtcgat gtcagaaggg
    40981 tatctggaag atgacgccac ggtggagtgc ccgctacacg ccgccagttt ttgcctgaaa
    41041 acggggaaag cgttatgcct gcccgccacc gatccgctca ccacttatcc agtacacgtt
    41101 gaaggtggtg acattttcat cgacttaccg gaggcgcagc catgagcgat ctgcataacg
    41161 agtccatttt tattaccggc ggcggatcgg gattagggct ggcgctggtc gagcgattta
    41221 tcgaagaagg cgcgcaagtt gccacgctgg aactgtcggc ggcaaaagtc gccagtctgc
    41281 gtcagcgatt tggcgaacat attctggcgg tggaaggtaa cgtgacctgt tatgccgatt
    41341 atcaacgcgc ggtcgatcag atcctgactc gttccggcaa gctggattgt tttatcggca
    41401 atgcaggcat ctgggatcac aatgcctcac tggttaatac tcccgcagag acgctcgaaa
    41461 ccggcttcca cgagctgttt aacgtcaacg tactcggtta cctgctgggc gcaaaagcct
    41521 gcgctccggc gttaatcgcc agtgaaggca gcatgatttt cacactgtca aatgccgcca
    41581 ggtatcctgg cggcggtggc ccgctgtaca ccgccagtaa acatgccgca accggactta
    41641 ttcgccaact ggcttatgaa ctggcaccga aagtgcgggt gaatggcgtc ggcccgtgtg
    41701 gtatggccag cgacctgcgc ggcccacagg cgctcgggca aagtgaaacc tcgataatgc
    41761 agtctctgac gccggagaaa attgccgcca ttttaccgct gcaatttttc ccgcaaccgg
    41821 cggattttac ggggccgtat gtgatgttgg catcgcggcg caataatcgc gcattaagcg
    41881 gtgtgatgat caacgctgat gcgggtttag cgattcgcgg cattcgccac gtagcggctg
    41941 ggctggatct ttaaggaagc acgatgaaag aaaaaacgat cattattgtc ggtggcgggc
    42001 aagcggcggc aatggctgcg gcctcgctac gccagcaagg gttcaccggt gagctgcatc
    42061 tgttttccga tgagcgacat cttccttatg aacgacctcc actctcgaaa tccatgttgc
    42121 tggaagattc cccgcagtta cagcaggtgt tacccgctaa ctggtggcag gaaaacaatg
    42181 ttcatctgca ttccggtgta accatcaaaa cgctgggtcg cgacacacga gagttagtgt
    42241 taaccaacgg cgaaagctgg cactgggatc agctttttat agcaatcggc gcggcagctc
    42301 gaccgctgcc gttgcttgat gcactgggag aacgctgctt taccctacgc catgccggtg
    42361 atgccgccag actgcgagaa gttctgcagc ccgaacggtc agtcgtgatt gtcggtgccg
    42421 gaactattgg tctggaactg gctgccagcg ccacgcagcg cagatgtaag gtgacagtga
    42481 ttgaactggc ggcaaccgtc atgggccgta atgcaccacc gcccgtgcaa cgctatcttt
    42541 tacagcgcca ccagcaggct ggtgtgcgca ttctgctcaa taatgccatt gaacatgtgg
    42601 tcgatggtga aaaagtagaa ctgacgctgc aaagtgggga aacgcttcag gctgatgtgg
    42661 tgatttacgg tattggtatc agcgccaacg agcaactggc tcgcgaggcc aaccttgata
    42721 ctgccaatgg cattgtcatt gatgaggctt gccgcacctg cgatcccgcg atctttgccg
    42781 gtggcgatgt ggcaatcact cgtcttgata atggtgcact acaccgctgc gaaagctggg
    42841 aaaacgccaa taaccaggcg caaattgccg ctgccgcaat gttagggcta ccgctaccgc
    42901 tactgccgcc gccgtggttc tggagcgatc agtacagtga taacttacag tttattggcg
    42961 atatgcgtgg cgatgactgg ctttgtcgtg gcaacccgga aactcagaag gcgatttggt
    43021 ttaatctgca aaacggcgtg cttatcggtg cggtcacgct gaatcagggg cgtgagattc
    43081 gcccaattcg caaatggatc cagagcggca aaacgtttga tgcgaaactg ctgatagatg
    43141 agaacatcgc gcttaaatca ctgtaaccag gataattagc gaatatctca acgcctgggg
    43201 cgtggcgagg tgcaagagtg tgtattacgt ttaaatcaca ttatcttgca aagggattgg
    43261 ttatgaacac acta
//
LOCUS       DAZOYL010000005        38336 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZOYL010000005 DAZOYL010000000
VERSION     DAZOYL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.005"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHUOY_000175"
     CDS             59..1423
                     /locus_tag="ACHUOY_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0681077.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUOY_000176"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUOY_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR0681078.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHUOY_000177"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHUOY_000177"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR0681079.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3673
                     /gene="yajL"
                     /locus_tag="ACHUOY_000178"
                     /pseudo
     CDS             3084..3673
                     /gene="yajL"
                     /locus_tag="ACHUOY_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
     gene            complement(3727..5175)
                     /gene="thiI"
                     /locus_tag="ACHUOY_000179"
     CDS             complement(3727..5175)
                     /gene="thiI"
                     /locus_tag="ACHUOY_000179"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR0681080.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5381..5623
                     /gene="xseB"
                     /locus_tag="ACHUOY_000180"
     CDS             5381..5623
                     /gene="xseB"
                     /locus_tag="ACHUOY_000180"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR0681081.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5623..6522
                     /gene="ispA"
                     /locus_tag="ACHUOY_000181"
     CDS             5623..6522
                     /gene="ispA"
                     /locus_tag="ACHUOY_000181"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR0681082.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6547..8409
                     /gene="dxs"
                     /locus_tag="ACHUOY_000182"
     CDS             6547..8409
                     /gene="dxs"
                     /locus_tag="ACHUOY_000182"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR0681083.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8464..9438
                     /gene="yajO"
                     /locus_tag="ACHUOY_000183"
     CDS             8464..9438
                     /gene="yajO"
                     /locus_tag="ACHUOY_000183"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR0681084.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9492..10010)
                     /gene="pgpA"
                     /locus_tag="ACHUOY_000184"
     CDS             complement(9492..10010)
                     /gene="pgpA"
                     /locus_tag="ACHUOY_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR0681085.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9988..10965)
                     /gene="thiL"
                     /locus_tag="ACHUOY_000185"
     CDS             complement(9988..10965)
                     /gene="thiL"
                     /locus_tag="ACHUOY_000185"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR0681086.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11043..11462)
                     /gene="nusB"
                     /locus_tag="ACHUOY_000186"
     CDS             complement(11043..11462)
                     /gene="nusB"
                     /locus_tag="ACHUOY_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR0681087.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11482..11952)
                     /gene="ribE"
                     /locus_tag="ACHUOY_000187"
     CDS             complement(11482..11952)
                     /gene="ribE"
                     /locus_tag="ACHUOY_000187"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR0681088.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12041..13144)
                     /gene="ribD"
                     /locus_tag="ACHUOY_000188"
     CDS             complement(12041..13144)
                     /gene="ribD"
                     /locus_tag="ACHUOY_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR0681089.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13148..13597)
                     /gene="nrdR"
                     /locus_tag="ACHUOY_000189"
     CDS             complement(13148..13597)
                     /gene="nrdR"
                     /locus_tag="ACHUOY_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR0681090.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13748..14287
                     /locus_tag="ACHUOY_000190"
     CDS             13748..14287
                     /locus_tag="ACHUOY_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0681091.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14586..15470
                     /gene="tsx"
                     /locus_tag="ACHUOY_000191"
     CDS             14586..15470
                     /gene="tsx"
                     /locus_tag="ACHUOY_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR0681092.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15647..15994)
                     /gene="yajD"
                     /locus_tag="ACHUOY_000192"
     CDS             complement(15647..15994)
                     /gene="yajD"
                     /locus_tag="ACHUOY_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR0681093.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16123..17094)
                     /gene="secF"
                     /locus_tag="ACHUOY_000193"
     CDS             complement(16123..17094)
                     /gene="secF"
                     /locus_tag="ACHUOY_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR0681094.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17105..18952)
                     /gene="secD"
                     /locus_tag="ACHUOY_000194"
     CDS             complement(17105..18952)
                     /gene="secD"
                     /locus_tag="ACHUOY_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR0681095.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18980..19312)
                     /gene="yajC"
                     /locus_tag="ACHUOY_000195"
     CDS             complement(18980..19312)
                     /gene="yajC"
                     /locus_tag="ACHUOY_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR0681096.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19335..20462)
                     /gene="tgt"
                     /locus_tag="ACHUOY_000196"
     CDS             complement(19335..20462)
                     /gene="tgt"
                     /locus_tag="ACHUOY_000196"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR0681097.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20518..21588)
                     /gene="queA"
                     /locus_tag="ACHUOY_000197"
     CDS             complement(20518..21588)
                     /gene="queA"
                     /locus_tag="ACHUOY_000197"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR0681098.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21681..22262
                     /gene="acpH"
                     /locus_tag="ACHUOY_000198"
     CDS             21681..22262
                     /gene="acpH"
                     /locus_tag="ACHUOY_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR0681099.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22267..24084)
                     /gene="malZ"
                     /locus_tag="ACHUOY_000199"
     CDS             complement(22267..24084)
                     /gene="malZ"
                     /locus_tag="ACHUOY_000199"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR0681100.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24240..25613)
                     /gene="proY"
                     /locus_tag="ACHUOY_000200"
     CDS             complement(24240..25613)
                     /gene="proY"
                     /locus_tag="ACHUOY_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR0681101.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25689..27008)
                     /gene="brnQ"
                     /locus_tag="ACHUOY_000201"
     CDS             complement(25689..27008)
                     /gene="brnQ"
                     /locus_tag="ACHUOY_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR0681102.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27415..28710)
                     /gene="phoR"
                     /locus_tag="ACHUOY_000202"
     CDS             complement(27415..28710)
                     /gene="phoR"
                     /locus_tag="ACHUOY_000202"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR0681103.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28768..29457)
                     /gene="phoB"
                     /locus_tag="ACHUOY_000203"
     CDS             complement(28768..29457)
                     /gene="phoB"
                     /locus_tag="ACHUOY_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR0681104.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29647..30849
                     /gene="sbcD"
                     /locus_tag="ACHUOY_000204"
     CDS             29647..30849
                     /gene="sbcD"
                     /locus_tag="ACHUOY_000204"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR0681105.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30846..33989
                     /gene="sbcC"
                     /locus_tag="ACHUOY_000205"
     CDS             30846..33989
                     /gene="sbcC"
                     /locus_tag="ACHUOY_000205"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR0681106.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34115..35299
                     /gene="araJ"
                     /locus_tag="ACHUOY_000206"
     CDS             34115..35299
                     /gene="araJ"
                     /locus_tag="ACHUOY_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR0681107.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35442..36350)
                     /gene="mak"
                     /locus_tag="ACHUOY_000207"
     CDS             complement(35442..36350)
                     /gene="mak"
                     /locus_tag="ACHUOY_000207"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR0681108.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36475..37386
                     /gene="rdgC"
                     /locus_tag="ACHUOY_000208"
     CDS             36475..37386
                     /gene="rdgC"
                     /locus_tag="ACHUOY_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR0681109.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37464..37919)
                     /locus_tag="ACHUOY_000209"
     CDS             complement(37464..37919)
                     /locus_tag="ACHUOY_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681110.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37916..38251)
                     /locus_tag="ACHUOY_000210"
                     /pseudo
     CDS             complement(37916..38251)
                     /locus_tag="ACHUOY_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9471 a        10314 c         9728 g         8823 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gcgaggtggc tgatggcgaa tatgacgtga tcgtactgcc
     3301 tggtggcatt aaaggcgcag agtgttttcg cgatagtacc ctgctggttg aaaccgttaa
     3361 acagttccac cgttccgggc gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct
     3421 ggtaccgcac gatatcttcc cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa
     3481 aattcccgcc gaacaatggc aggacaagcg cgtcgtctgg gatgcacggg taaaattgct
     3541 gaccagccag gggccgggta cggctattga ttttggtctg aaaattatcg acctgctggt
     3601 tgggcgcgag aaaacccatg aagtggcatc acaactggtg atggcggcag ggatttataa
     3661 ttattacgag tagtgtcgga tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac
     3721 cacaaattat gggcgataca ccttcacatt gttaaagccc tgctcgcgca gatagagcgc
     3781 ctgcagacgg ctcatcaccc cgcgctcaca ccacagtagc caggttctgt tctggtcgag
     3841 atcgccaaat ttggtgctca gtttatagaa cggcagagaa accacgtcga taccttcaac
     3901 cttcagcggc ttatcttcct gctcatcgac agaacggata tcgaggatca cgtcgttcgg
     3961 gccaaagcca ttgacggttt ccacttccac cacttcctgc tcggtctgct gggcgatttc
     4021 gcggatatca acgttattcg cttcctcaac cactttatcg agaatactga agtcgaactt
     4081 ctcttcttcc gcttcaatct tcgatttaac cgctttcacc gtcgggcttt tggagatcac
     4141 gccacaatat tctggcatcg tgcgagcaaa gtcttcagtg ccaatctggc gggccaggtt
     4201 gatgatgtgc tctttgtcgt aagaaatcag cggacgcagg atcagcgtat cagagacgtt
     4261 atcaatcagg cgcaggttgg tcagcgtctg actggacacc tggccgagcg cttcgccggt
     4321 gaccagcgcc tgtacgccgt aacgttcagc cactttagac gcggcacgca ccatcatacg
     4381 tttgaggata acgcccatct gaccgtcgtc gattttctcg agaatttccc cgacgaccgg
     4441 ttcgaaatta atggcgacaa aacgcacgcg gtgggagctg ccaaagcggt tccacagata
     4501 atgcgccacc tgacgcacgc caatttcatg cgctgcgccg ccgaggttaa agaagcagta
     4561 atgcacgcgg cagccgcgac gcatcaacat ataactggaa acaccggagt cgaaaccacc
     4621 ggaaatgagc gacagcacat cttcctgggt gccaatcggg aaaccgccaa taccttcgta
     4681 gcggccttta atcagcagaa gacgatcgtc ttccacttcc agatggacag ttacatccgg
     4741 gttagtcagc ttcacgcgcg cggattcaat atgctgattt aaaccgccgc cgacgtaacg
     4801 ttccacatca atcgagctaa aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt
     4861 tttgccttcc agctgatcgc gatattgaac caacgctttc tcgaaaatat cgtgcatgtc
     4921 ggtaaacggc acgtcttcga cttcgagaat atggtggata cccggaatac gggtcagggc
     4981 gtcgcgaata gccagacgct gtttttcatc ttttgcgcga acttcgatgt tatcccagtg
     5041 gcggacgaca gcgagcgtct catcatagtg ctttaaaacg ttacgaatgt tcccggtaag
     5101 gatttttata aagcgcaagc gcacagattg gcttttgatg gtgatttccg ggaacaattt
     5161 aatgataaac ttcatggcgg caatggttcg ttggcaagcc ttaagcgact tgtataggga
     5221 aaaatacagc agcccacacc tgcggctgca tccaggcgcg gaagtatacc actaacatcg
     5281 ctttgctgcg cacatcacct taccattgcg cgttatttgc tatttgccct gagtccgtta
     5341 ccatgacggg gcgaaaaata ttgagagtca gacattcatt atgccgaaga aaaatgaggc
     5401 gcccgccagc tttgaaaagg cgctgagcga gctggaacag attgtaaccc gtctggaaag
     5461 tggcgacctg ccgctggaag aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg
     5521 tcaggggcag gccaaattac aacaagccga acagcgcgta caaattctgc tgtctgacaa
     5581 tgaagacacc tctctacccc cttttacacc ggacaatgag taatggactt tccgcagcaa
     5641 cttgaagcct gcgttaagca ggccaaccag gcgctgagcc gttttatcgc cccactgccc
     5701 tttcagaaca ctcccgtggt cgaaaccatg cagtatggcg cattattagg tggtaagcgc
     5761 ctgcgacctt ttctggttta tgccaccggt catatgttcg gcgttagcac aaacacgctg
     5821 gacgcacccg ctgccgccgt tgagtgtatc cacgcttact cattaattca tgatgattta
     5881 ccggcgatgg atgatgacga tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc
     5941 gaagcaaacg cgattctcgc tggcgacgct ttacaaacgc tggcgttctc gattttaagc
     6001 gatgccgata tgccggaagt gtcggaccgc gacagaattt cgatgatttc agaactggca
     6061 agcgccagcg gtattgccgg aatgtgcggt ggtcaggcat tagatttaga tgcggaaggc
     6121 aaacacgtac ctctggacgc actcgagcgt attcatcgtc ataaaaccgg cgcattgatt
     6181 cgcgccgccg ttcgccttgg tgcattaagc gccggagata aagggcgtcg tgctctgcca
     6241 gtactcgaca agtacgcaga gagcatcggc cttgccttcc aggttcagga tgacatcctg
     6301 gatgtggtgg gagatactgc aacgttggga aaacgccagg gtgccgacca gcaacttggt
     6361 aaaagtacct accctgcact tctgggtctt gagcaagccc ggaagaaagc ccgggatctg
     6421 atcgacgatg cccgccagtc gctgaaacaa ctggctgaac aatcactcga tacctcggca
     6481 ctggaagcgc tagcggacta catcatccag cgtaataaat aaacaataag tattaatagg
     6541 cccctgatga gttttgatat tgccaaatac ccgaccctgg cactggtcga ctccacccag
     6601 gagttacgac tgttgccgaa agagagttta ccgaaactct gcgacgaact gcgccgctat
     6661 ttactcgaca gcgtgagccg ttccagcggg cacttcgcct ccgggctggg cacggtcgaa
     6721 ctgaccgtgg cgctgcacta tgtctacaac accccgtttg accaattgat ttgggatgtg
     6781 gggcatcagg cttatccgca taaaattttg accggacgcc gcgacaaaat cggcaccatc
     6841 cgtcagaaag gcggtctgca tccgttcccg tggcgcggcg aaagcgaata tgacgtatta
     6901 agcgtcgggc attcatcaac ctccatcagt gccggaattg gtattgcggt tactgccgag
     6961 aaagaaggca aaaatcgccg caccgtctgt gtcattggcg atggcgcgat taccgctggc
     7021 atggcgtttg aagcgatgaa tcacgcgggc gatatccgtc ctgatatgct ggtggtcctc
     7081 aacgacaatg aaatgtcgat ttccgaaaat gtcggcgcgc tcaataacca tctggcacag
     7141 ctgctttccg gtaagcttta ctcttcgctg cgcgaaggcg ggaaaaaagt tttctctggc
     7201 gttccgccaa ttaaagagct gctcaaacgt accgaagaac atattaaagg catggtagtg
     7261 cctggcacgt tgtttgaaga gctgggcttt aactacatcg gcccggttga cggtcacgat
     7321 gtgctggggc ttatcaccac gctgaagaac atgcgcgacc tgaaaggccc gcagttcctg
     7381 catatcatga ccaaaaaagg tcgtggttat gaaccggcag aaaaagaccc catcactttc
     7441 cacgccgtgc ctaaatttga tccctccagc ggttgtttgc cgaaaagtag cggcggtttg
     7501 ccgagctatt caaaaatctt tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag
     7561 ctgatggcga ttactccggc gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa
     7621 ttcccggatc gttacttcga cgtggcaatc gccgagcaac acgcggtgac ctttgccgcc
     7681 ggtctggcga ttggtgggta caaacccatt gtcgcgattt actccacttt cctgcaacgc
     7741 gcctatgatc aggtgctgca tgacgtggcg attcaaaagc tcccggtcct gttcgccatc
     7801 gaccgcgcgg gcattgttgg tgctgacggt caaacccatc agggcgcttt tgacctctct
     7861 tacctgcgct gcataccgga aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc
     7921 cagatgctct ataccggcta tcactataac gacggcccgt ccgcggtgcg ctacccgcgc
     7981 ggtaacgcgg ttggcgtgga actgacgccg ctggaaaaac tgccaattgg caaaggcatt
     8041 gtgaagcgtc gtggcgagaa actggcgatc cttaactttg gtacgctgat gccagacgcg
     8101 gcgaaagtcg ctgaatcgct gaacgctacg ctggtcgata tgcgttttgt gaaaccgctt
     8161 gatgaagcgt taattctgga aatggccgcc agccatgaag cgctggtcac cgtagaagaa
     8221 aacgccatta tgggcggcgc aggcagcggc gtgaacgaag tgctaatggc ccatcgtaaa
     8281 ccagtacccg tgctgaacat tggcctgcct gacttcttta ttccacaagg aactcaggaa
     8341 gaaatgcgcg ccgaactcgg cctcgatgcc gccggtatgg aagccaaaat caaggcctgg
     8401 ctggcataat ccctactcca ctcctgctat gcttaagaaa ttattcatgg caggagtggc
     8461 agcatgcaat acaacccctt aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc
     8521 tgtatgacct ttggcgagcc agatcgtggt aatcacgcat ggacactacc ggaagagagt
     8581 agccgtccca tcattaaacg cgcgctggaa ggcggcataa atttctttga taccgccaat
     8641 agctattccg atggcagcag cgaagagatc gttggtcgcg cactgcggga tttcgcccgt
     8701 cgtgaagacg tggtcgttgc gacgaaagtg ttccatcgcg ttggtgattt accggaagga
     8761 ttatcccgtg cacaaatttt gcgctctatc aacgacagcc tgcgccgtct cggcatggat
     8821 tatgtcgata tcctgcaaat tcatcgctgg gattacaaca cgccgatcga agagacgctg
     8881 gaagccctga acgacgtggt aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg
     8941 cacgcttcgc agtttgctca ggccctgaag ctacaaaaac agcacggctg ggcgcagttt
     9001 atcagtatgc aggatcacta caatctgatt tatcgcgaag aagagcgcga gatgctgcct
     9061 ctgtgttatc aggagggcgt ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg
     9121 acgcgtccgt ggggagaaac taccgcacga ctggtgtctg atgaggtggg gaaaaatctc
     9181 tataaagaaa gcgatgaaaa tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa
     9241 gaactcggtg caacacgagc acaagttgcg ctggcctggt tattgagtaa accgggcatt
     9301 gccgcaccga ttatcggtac atcgcgggaa gaacagcttg atgagctgct gaacgcggtg
     9361 gatatcactt tgaagccaga acagattgcc gaactggaaa cgccgtataa accgcatgcg
     9421 gtcgtaggat ttaaataaga gtccaggcct gatgagacgt gacaagcgtc acatcaggca
     9481 tcggtgcaca actacgacag aatacccagc ggccagtgat gaccgataaa gtacaggatg
     9541 cctgcggaaa tcaccccggc gacaatatcg tcgatcatga tccccatgcc gccatgtaca
     9601 ttgcgatcaa accagcggat cggccacggc ttccacatat ccagaatacg gaaaatcaca
     9661 aacccggcgg caacccactg ccagtcattg gtcggcagcg ccatgagcgt gatccacata
     9721 ccaataaatt cgtcccagac aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt
     9781 tgatgacaaa gatagacgcc gatacagatc cccaacatca ccaccagcga gtagagctgc
     9841 cagggtaaaa aggtcatcag ataccagaac ggaatcgctg ccagcgatcc catcgtgcca
     9901 ggaacgattg ggcttaatcc actcccgaat ccgacagcaa gcagatgcca cggattactc
     9961 atcttcaggc gacttttcgc gacatcttta tggcgtggca aaatggtcat atcctttcca
    10021 gtcaaacgtg acaggttcgc cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat
    10081 ttgcccgata caggtaaacg gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg
    10141 gttcagttcc ggcaccgtga aacacaactc gtaatcttca ccgccagaga gcgcccagcg
    10201 cagcgcctgt tccggttcaa catggcgaga aagcgcatca gaaaacggca gcaatgccag
    10261 gtcaatacgt gcgccgcagt cgctggcttt cacgatatgc ccgagatcgg aaatcagacc
    10321 gtcagagaga tcgatagctg aatttgccag atcgcgcagt gcctgcccct gtaaaatacg
    10381 cggcgatgga cggagatgac gtttgatcaa gtagtcagca tctttagcat cggcaacctg
    10441 caaacggttt tgcaaaatcg ccagcccggc ggcgctatcg cccggtgtac cggtcacata
    10501 gatccagtca cccggtttcg ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc
    10561 gtggataccc aacgtcattg ataatggccc acgcgtggta tcgccgccaa tgagttgcat
    10621 atcgtaataa ttgagaagat caaacaaact gtcgctgaag gactcaagcc acgcttcgtc
    10681 tacgtccggt aaggttaatg ccagcgtcag ccaggccgga tcggccccca tcgctgccag
    10741 atcgcttagg ttcaccgcca gtgctttata agccagatca gcaggatcga tatcagggag
    10801 gaaatggtta cccgccacca gcgtatcagt gctgatcgcc agggtctgtt tctcggggat
    10861 attgagaagt gcgcaatcgt cgccgatgcc cagttcgaca tcaagacgag aacttcttac
    10921 acggtcaaaa taacgggcaa tcagggagaa ctcgccacat gccatacgtt atgcctcagc
    10981 aggtaaaaaa gaaaaggccg acgatcatgg aacggtcttc cgtgaatcta ccggcctgga
    11041 tatcactttt tgttagggcg aatcacaggt gctgctttat cgagtacgcc gttgacgaac
    11101 ttatggctgt cttctgcgcc gaacgatttc gccagttcga tcgcttcgtt aatggccact
    11161 ttgtatggca catcgctacg tttagacagt tcgtacagcg caatgcgcag tactgctttt
    11221 tctacctgtc ccagttcttc cagcaggcgg gacaggtatg gcttcatcag tccgtcgagg
    11281 tatgcggtat tagtcgccac cccggccagc agctcacgga agtacaggac gtcaacgtct
    11341 tttacatcct gttcagccag gaactggtat tcaacatcag cgatgtcgtt ctgggacaac
    11401 tgccaggagt agagcgcctg gacggcacac tcacgagcgc ggcgacgagc agcaggtttc
    11461 acggatttcc ccttactaat ttcaggcctt gatggctttc aatacattaa tcatttcaag
    11521 cgcggtcagt gcagcttctg cacctttgtt gccagctttg gtgccagcac gttcgatcgc
    11581 ttgttcaatg ctttcagtgg tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc
    11641 aacatgcgcc aggccgttgc ttgcaccacc agcgacatat tcaaagtggg cagtgccacc
    11701 acgaataacc gtacccagcg caatcaccgc gtcgtattta ccggttttag ccagtgcacc
    11761 cgccgccagc ggcagctcat aggcaccagg cacccaaaca acggtaatgt tttcatcttt
    11821 tacctgaccg atacgtttca gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa
    11881 gttgttgaaa cgcgcgatgg tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc
    11941 aataatgttc attttcttcc ttcgggttcg agtatggccc cgcagggggg cggattttag
    12001 cataatattt cgtgcgctgc ttccctttcg agccgggaga tcatgcaccc actaaatgca
    12061 ggcaaacatc cgggcctaca tgacgtatct ctttgaattt aaattggggg gcgtcggcta
    12121 atttctcaag ccctggcagc gtgcataatc cgcgggcgtc gctgcctaat agtttaggtg
    12181 cgatatagac aatcagctca tcgactaaac ccgcctgcag caatgcgcca gcgagcgttg
    12241 gccccgcttc cacccagatg ctgttaattt gctgtttacc cagttgcatc atcagtacaa
    12301 ccagatccag atgaccttta tgctctggaa tcagcaaggt acgcaccgtt tccggccact
    12361 cactagaatc ttcctgcgta cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat
    12421 gttccggcgt cacgcgattt tggctatcaa tcacaatacg tactggctgg cggagatttt
    12481 gttgcgggta gagcgcctga gtttgttcat ccagttcaga ccaacgcact gttaaggctg
    12541 gatcatccgc cagcaccgtg gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc
    12601 gttgtacgtc gcgccgcgcc tgaggcgaag tgatccactg gctttcgccg ctcgccatcg
    12661 ccgtgcggcc atcaagcgat gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca
    12721 tccgcttgag aaagccttta ttcaattgct cggcttcact catcatcagc ccgtggctga
    12781 cgtcaatgcc agcctgttgc agacggtaaa gtccacgccc agcgacctgc gggttaggat
    12841 cctgcatcgc tgcgaccacg cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg
    12901 gcgtacggcc atgatggcta cagggttcga gtgtcacata cgccgttgcg cctttggctt
    12961 tttcacccgc catacgcaac gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt
    13021 aaccttcacc gacaatttcg ccatctttga caatgacgcc cccgacattc gggttggggt
    13081 gggtggtaaa acgtcctcgt tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct
    13141 gcacggctta gtcctccagg cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac
    13201 tgcggtagac agaggcaaaa cggatatagg cgactttatc gagctttttc aattgctcca
    13261 tcaccagatt gccaatcatc ttgctcggta cttcgcgctc accggtggcg cgcagctgcg
    13321 atttaatatg attgattgcc atttcaacgt catcggaact caccggacgt ttttccagcg
    13381 cccgcagcat tccgctacgc aatttctctt cattaaacgg ttcacgcacg tcgttgcttt
    13441 ttacaacacg cggcataacc agctccgcca cttcaaaggt ggtgaaacgt tcattacaca
    13501 ccagacactg ccgacggcgg cgtacggatg aaccctcgcc cacgagacga gagtcaatta
    13561 ccttagtgtc cacggcgaaa cagaatgggc aatgcatacg gcgtcctgac caggtggtta
    13621 acggaaatct attttaccct gaactgacga tacaacaaag gcgcaacgct tttgagataa
    13681 aggatcgatg ctttcgctgc ttttaccctc taccattagg ctaattgcga cgagaaaggg
    13741 acgacctatg aatactaacg tttttcgact gctcctgctg ggaagcctgt tcagccttag
    13801 cgcctgtgtg cagcaaagtg aagtgcgaca gatgaaacac agcgtcagca cgctgaacca
    13861 ggagatgacg caactcaatc aagaaacagt caaaatcact cagcaaaaca ggctgaatgc
    13921 aaaatccagc agtggggttt accttctgcc tggagcgaaa acaccggcaa gactggaaag
    13981 ccagatcggt actttacgta tgtcgctggt gaatattacg cctgatacag atggcaccac
    14041 ccttacacta cgtattcagg gtgagtcgaa tgatcccttg cccgcattca gcggaactgt
    14101 tgaatatggg caaattcagg gaacaataga caactttcag gaaatcaatg tgcagaatca
    14161 attgattaat gcccctgcca gcgtcctcgc ccccagcgat gttgatattc cgttacaatt
    14221 aaaggggatt tctgtggatc agttaggctt tgtacgtatc cacgacattc aacctgttat
    14281 gcagtaaacg tatttcggga cgattttgtg cgtcccgcaa catctttccc cgtcattttg
    14341 ttactctgct tacatcacct ggattgatag taaaagtttg caacaagggc gaaagtcagt
    14401 acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact
    14461 gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc tgtatacgaa
    14521 atatttagaa acgcaatttg cgcctttttc actcccgcaa gggattttca aacagtggca
    14581 tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct tcgtctttta
    14641 ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg caccagagcg
    14701 ttaacgttgt cggaagctat cacacccgtt tcggaccgca gatccgcaac gatacctacc
    14761 ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat gcggatgcgc
    14821 cggtattctt cggcggtaac tccgatgcta aaggtatctg gaaccacggt tctccgctgt
    14881 ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac cttagcttcg
    14941 gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt cgtaataaag
    15001 atggtcgcca gagcacctgg tacatgggtc tgggtaccga catcgatact ggtctgccga
    15061 tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatgac gcagcgaacg
    15121 aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt
    15181 ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc gatttagggg
    15241 atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct atcgcttcca
    15301 gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct cgttactggc
    15361 acgacggtgg tcagtggaac gacgatgcag aactgaactt cggcaacggc aacttcaacg
    15421 ttcgctctac cggctggggt ggttacctgg tagtaggtta caacttctga ttatgaaaat
    15481 gccgggattt attcccggca tttctgattg ttaacaacgt gtgctttgtt catgccggat
    15541 gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa ttcaatatat tgctgaaata
    15601 acgtaggcct gataagcgta gcgcatcagg caattttacg ttttaatcac ttcttcttgt
    15661 tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg
    15721 catcttcccc tgcgataacg gtcgtaccat actgatccgc ttcggtatat ttcgaatgct
    15781 catgatcgtg gcaatagaga caaagcaatt cccagttact gccatcttcc gggttattgg
    15841 tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag gttggaataa acaaactcgc
    15901 gggaacagcg accgcagacc cacggataga tttttaatgc tttttcgcga tagccgcttt
    15961 ctaaccgcgc atagtttttt gggatgatag ccatgaaaag ttacctggat gaaatgtagg
    16021 gttacgcgca gtttcccaag atgcggggat tttcgcaatc tccatacatc aaaaaaaatc
    16081 ccgatcttct gaccgggatt ttcaacatca acgggaactt gattacggca gaattgacgg
    16141 ctgatccgcc ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag
    16201 tttcagagcc aacgcagatg ctacgtagat ggaagatgca gtgccgatgg aaacaccgat
    16261 aagcatggtc agcgagaagc cttccagtac cggaccaccg aagagataca gcatcaggat
    16321 aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac
    16381 gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac gaattcggtc
    16441 cgataccacg atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat
    16501 ggtcaggtca atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg
    16561 cgccagcgca ataaccaccc ctgccgccag tcgccactca aagcggaaac ctacgtacac
    16621 gaggatagac agcagcgctg ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc
    16681 cacgctcgga ccgacgaact caatacgctt cactgctgca ttctgattgg tggattcgtt
    16741 aatcaccttc agaacctggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg
    16801 catacggacc atgatgtcgt ggctgctacc aaagttttgc agcattggct cttcaaaacc
    16861 ggctttttgc aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc
    16921 gataaccgta ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac
    16981 gatagcagcg attaacagca gaccagagat gccgaaagcc cagtagtccc agcgcataaa
    17041 gtcatagact ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca
    17101 ctcctcagat tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac
    17161 gcgtaccgac aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga
    17221 accctttaat tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga
    17281 tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa
    17341 cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga
    17401 ccgctaccgc aagggttaag acgatacccg cgatacctgg catactcagt gttgcgcctg
    17461 gcaacagcga cataataccg acgatcaaga tcaagttggc aatcagagca ctggtcgcaa
    17521 tcagaccaaa cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa
    17581 ggcaagcctc cagcccctgc tcaatgttct gcatacccag ggtcgggcca attgtacgtt
    17641 cttcaacaat ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gacagctgac
    17701 gggcttcgtt cgggttgttg atgccggtga tacggaagct gttacccaga cgagactgga
    17761 tgttggcgat gttaatcacc tcttcctgtt tcaccagaac cgcacgacca tttgcatctt
    17821 tcttaccgct gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct
    17881 tggtgaagtt agacataatg ttgccaccag cgctatcgag cgagatgtta acctgcggct
    17941 ggttgtattc gtcctggctg gaagtggagt cggtgatatg gtcaccggtc agaattacgc
    18001 gtttgtacag cacaaccggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta
    18061 cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt taccagacgg aattccagcg
    18121 ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc ctgaatacca ggcagttcaa
    18181 caacgatacg gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta
    18241 cacggttacg caggatatta atgttctgct gcaccgcata ttcacgcgct tcactcagac
    18301 gagcatcgct cattaccgca cgcagctggt tgctgccctg gctgctaatc accaggtccg
    18361 gatggcgctt gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtga
    18421 tgctcaggcc gtagttgttt tctttacgaa cagtggtata cgggatgccc ttttcgcgca
    18481 ggtcactgcg taggctatcg atattttgtt cctggagttt gccaagcgcg gtgtccatat
    18541 ccacttccat caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag
    18601 catgaatagc tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt
    18661 tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga
    18721 agcgcgcaag aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt
    18781 gtaacgtttt ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag
    18841 tgatctgaac agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga
    18901 caatcaccac gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc
    18961 ccttagggaa aaattttaat tacagcgcct tcatggtgcc tttcggcagg acggcagcta
    19021 cgaagtcacg tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt
    19081 tttccgctac tttggttacg cgaccaacca ggccaccgtt cgtcagaact tcatcacctt
    19141 tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga
    19201 tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac
    19261 cttgcgccgg tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc
    19321 cctcattatt aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa
    19381 aaatcagtta cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc
    19441 aaacgctggt agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg
    19501 caacggtcaa gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac
    19561 tcaggatcaa gtgggccagt atcgctctta tacttcgcat tgcggatttt caccacgcca
    19621 tcggtcacga acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata
    19681 tcgataccac gacgtacgcc ttcaaccagg tcttctggtt taccaacgcc catcaggtaa
    19741 cgcggtttgt ctgccggaat ttgcgggcat acatgctcca gaatgcggtg catatctgct
    19801 ttcggctcac ccacagccag accgccgaca gcgtagccat caaaaccgat atctaccaga
    19861 cctttaacag aaatatcacg taaatcttcg taaacgctgc cctgaatgat accaaacagc
    19921 gcatttttgt ttccgagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac
    19981 atctccatgg agcgttttgc gtaatcccag tcagcaggat acggcgtaca ctcatcaaag
    20041 atcatgacga tatccgaacc aagatcgtac tgaatctcca ttgatttttc aggatcgagg
    20101 aaaatcggat cgccgttgat cgggttacgg aagtgcacgc cctgttcggt gattttacga
    20161 atatcgccaa ggctgaagac ctggaagccg ccggagtcgg tgaggatcgg ccccttccac
    20221 tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg gcgcagccac
    20281 aggtggaagg tgttgccgag gataatttgc gcgccagtgg cttcaacttc ttccggcgtc
    20341 atccctttta cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc cactacgcca
    20401 cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc
    20461 attttttctc cactacgtca gaaaaacagt ccaacgtttt aaaccagcgc cacggaatta
    20521 ctccccgacg cgctcattaa ttgcctgcgg attgtacgtg ataaacatcg catcaccgta
    20581 actaaaaaag cgatatttct cttctaccgc tgctttatag gcgttcatgg tgtgttgata
    20641 accggcaaag gccgaaacca gcataatcag cgtcgattcc ggcaggtgga agtttgtcac
    20701 cagcgcatcg accactttgt actggaagcc cggatagata aagatttggg tatcgtcaaa
    20761 gaacggttcg ataagatcgt ttttcgccgc ctgagccgcg ctttccagcg aacgtactga
    20821 ggtggtgcca accgcaatca cccggttacc gcgcgctttc gccgccagta ccgcgtctac
    20881 cacatcctgc ggcacttcag cgtattccga gtgcatgatg tgatcttcaa tggtgtcgac
    20941 gcgcaccggc tggaaggtgc ccgcaccaac gtgcaacgtc acaaacgcca tctccacgcc
    21001 tttggcgcgt aatttttcca gcaaaggttc gtcaaaatgc agacctgcgg tcggggctgc
    21061 aaccgcgccc ggtttttcgc tataaacggt ttgataaagt tcgcggtcag cgtcttcgtc
    21121 cggacggtcg atatacggcg gcagcggcat atggccgatg ctgttgagaa tatccagcac
    21181 cgagcgttca tcattaaatt cgacttcaaa cagtgcgccg tggcgcgcgg tcattgttgc
    21241 gttaatactt tcgtcatcgc ccagcagcag ttctgcgcca ggttttggcg ctttcgaggc
    21301 gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgt tcaaccagca cttcaatctt
    21361 gccgccgctg gctttacgcc caaacaggcg cgccgggatc acgcgggtat tattaaaaac
    21421 cagaagatcg ccggggttga gcttatcaag taaatcggtg aaagtaccgt gcgtcagcgc
    21481 gcccgtcggc ccgtccagcg acagtaaacg acagctactg cgttcaggca tgggatagtg
    21541 ggcaatcagg gattcgggca attcaaagga gaaatcggta acgcgcatga cactgactca
    21601 gactaaaata agaggcgggt agtctagtgc cggggcgctc tccctgcaac ctttaccccc
    21661 ttttggatag aatataatcg atgaattttt tagctcacct gcatttagcc catctcgcgg
    21721 aaagctcgct ttccggcaat ttactggctg atttcgtacg cggaaatcct gaagaaagtt
    21781 ttccgcccga cgtcgtggct ggcattcata tgcatcgacg tatcgacgta ttaaccgaca
    21841 atctgccgga agtccgcgaa gcacgggaat ggtttcgtag tgaaactcgc cgcgttgcgc
    21901 ctattacgct ggatgtcatg tgggatcact ttctttcccg ccactggtcg cagctgtcgc
    21961 cggactttcc gctacaggaa tttgtctgtt atgcccgcga gcaagtgatg acgattttgc
    22021 cggactcacc gccacgtttt atcaatctga acaattactt gtggtcagag cagtggctgg
    22081 tgcgctatcg cgatatggat ttcatccaga acgtgttaaa cggcatggca agccgccgcc
    22141 cacgtctgga tgccctgcgt gactcctggt acgatttaga cgctcattat gacgccctcg
    22201 aaacccgctt ctggcagttt tatccgcgga tgatggcgca ggcgtcacac aaggcgttat
    22261 aaaacgttag ttcatccata ccgtagccga aatggcaggc aaagccagaa tcccgtcagt
    22321 cagttgccca tgcctttctt tgcattgcca ttgcacggca ttgagcaacg gtgacgcggg
    22381 tagcacgact tcacaagctt cgccacggtt gattgccacc agtacacgct gctgattcag
    22441 cacgcggaca aataccacca cgttatcttc cgcatacagc acctgacagc cgccatgacg
    22501 tagcgcctga cttttcttac gcagcgcaat cattcgctgg tacagcgcga ataacgccgt
    22561 atcctgcttt tccacctgcc aggggaacgg tttacggcaa aacggatcgt ttttgccatc
    22621 cagtcctact tcatcaccgt aataaatgca cggtacacca ggccaggtga acagccagac
    22681 caccgccagc ggcaggcgcg caatatcccg accgagcagc gttttaaatc gcgcagtatc
    22741 gtggctgtcg agctgattaa acatacgtaa ttgttgttga tgagaaagcc ctgcgcggta
    22801 attatccatc caggccatac aggtttgggc atcaatttgc tgcggatcgt aagagatatc
    22861 ggtattggca agaaatcccc acaacgggaa tgtgaagcca cgatagttca tggcggcatc
    22921 ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa tgttcgccga caatatacgc
    22981 ttccggctgg gtttctttcg ccgcttcggt gctcccagca acgtgctgca tattattgcg
    23041 cgccccaccc gcctccccta gcatatgcac cacatccagc cgccagccgt ccatactcca
    23101 cggcgctttc agccaatggc ggacaatact gtcttccccg cgataaattt cattcaccag
    23161 actttccgac tgataatcca gcttcggcaa gctggcatag ccaagccagt cgagcgccgt
    23221 gccatcatca ctaaacgagt accagtcgcg ccagggcgat tcagggttgt gacaagctcc
    23281 ccccgtgcca cgattgtgcc tatcaaacca ggcatgggaa tcgccactgt ggttaaacac
    23341 gccatccagc cccagccgca ttcccagctg ctgcgtattg tggcgtaagc gcagcaacgc
    23401 cccatcaccg ccaaactgcg gatcgacatg gcgataatcc tcggtatcgt atttatgtac
    23461 gctgggagct ttaaacaccg gattgagata cagcgccgtc acgccaagct ttttcagata
    23521 cggcagtttt tcgcttatcc cgtccagatc gccgccatag aacgttgatc cgcccgcctg
    23581 cgccgtgacc ggttcatccc agtcacgcaa gatgatctct tgtccggctg catgatggta
    23641 atagacatga tcctgttcag cttcacgagg aagactacgc gcaaaacgat cagggaagat
    23701 ctgataaaaa atctgatccg cagcccattg tgggccgata tccggtacat cgacggcaaa
    23761 ctgctccagt cgtgccggcg gcattcggct gaagccctgc ggtgtaaacc aacgctggcg
    23821 atcgtgccac agcagtttga aactgtaacg ccgccggggt tgtccgctgg agagatcaat
    23881 cgccgcacgc catgcggtga cgccaggctg ctgctgactg cgatgcttat gcatcgatac
    23941 tgacatttct tcgttatcgt gttctgtacg cagcataatg cgctgcggtg ggtcttcgcc
    24001 cgtcagccac agtgtaatga gcagttgatc tttgctttgt ttaacaaatg ggggcaccgg
    24061 caggtgccat gcatttaaca tcataattcc cctgtgatga aaatctgatc accttgccat
    24121 agcctaatgc aggatcactc atcatctgaa ggatatttgg gggaggagga cgggggcgga
    24181 taactttcgg aatagccgga tcaggatttc ctcctgaacc ggcttacaga acgcaaggat
    24241 tactggtttt cagccagctg acgatcgtgg cgacgcttaa acatccagcc aatcaacagc
    24301 acaacaatcc acgcgaaacc gacatacagc gagatacgcg tatccgggtg ataaccaatc
    24361 aacccgataa taaagagcag gaaaatcagc ccgccgatgg tcgttgctac cccacccggc
    24421 actttaaatt tcagcgcctt aacttcttct ggcggcaaac agcgacggaa ggcaatttgc
    24481 gacagcagga tcataatcca cacccacacc gtggcgaagg ttgccagcga agcgatcacc
    24541 aggaagacgt tttccggcat gatgtagttc agatacaccg caaacagcag cgcggtagtc
    24601 atcaccagca ccgtaaccca cggaataccg cgacgcgacg ttttgctgaa aattttcggc
    24661 gcgctgccct gctctgccat accgtggagc atacggccta cgccaaagac gtcactgtta
    24721 attgccgata gcgaagcggt cagcacaaca aagttaagaa tgctagcggc aaaggtaatg
    24781 cccatatgct ggaacgtcag cacgaacggg ctaccggcag tgccaacctg attccacggg
    24841 tagatagaca taatgacgaa cagcgtaccg acgtagaaca ccagaatacg catcggcacg
    24901 gagttaatcg cacgcggtat cgatttctca ggatctttcg cttcaccggc ggtaatcccg
    24961 ataatttcga tcccaccgta agcaaacatc accatttgca acgacatcac catcccaagc
    25021 cagccgttac tgaagaagcc gccgttgctc cacagattat gaataccggt cggttgcccg
    25081 ccgttgccaa tcccccagat gatgatgccg aaaccagcga caatcatgat gatgatggtg
    25141 gcgactttaa agaacgagaa ccagaattcc agctccccga agaccttcac gctcatcagg
    25201 tttacggcgc aaatgatcag caccacgctc agtacccaaa tccagtgcgg caccgtcggg
    25261 aaccagacac ccatatagat accaaaagcg gtcacatcgg cgatggcgac aataaggatt
    25321 tcaaagcagt aggtccagcc ggtaatgtaa cctgccagcg ggccgaggtt ttcctgcgca
    25381 taacgcgaga aagagctggc ggccgggtta tgtaccgaca tttcccccag cgcacgcata
    25441 atgatatacg ccgcgatacc accgataata taggccaaca acacgctcgg accggccatt
    25501 ttgatggcgt ctgccgaacc gtaaaacagc ccggtaccaa ttgctgaacc cagtgccata
    25561 aagcgtatgt ggcgggtact tagcccacgc tttagcttgt tcttactttc catcaattcc
    25621 taacccatca acacaataaa aaaccacggg gcatcgcagc cccgtggtta aaacaaatgt
    25681 tcagtgattt agtgagcgct ggaggtcacc tgacgacctg ccgcgcgatc ccagataatg
    25741 gccagaacca ccatcaccac tgttggcatt aaccacgcca gaccttgttc ggccagcggt
    25801 aaacgctgcg cccaggacgg taagatatcg ctgaatgcag atgctttgat cccgtcgaga
    25861 ataccaaaaa gcaggctgat aaacatcggc ggagcaatca cgcgggacga attatgccac
    25921 catgagcgtg taaaacttaa tacaaccagt gcgatacacg gcggataaat agcggtcagc
    25981 accggtacgg agatctggat cagctggctt aagccgaggt tagaaaccac catcgagaag
    26041 ccgccgagga taaacaccag cgtacgataa gagagcggta cgtactgggc aaagaattct
    26101 gcacaagcac aggtcaggcc aactgccgtt accaagcagg cgatgaagat taacgccgcc
    26161 agcaggaagc taccgccgcc gccaaaggtg tgctgaacgt aagcatgcag aatagcagcg
    26221 ccgtttgcag actgatcgac cagcgacgcg ctgtctgaac ccagacggaa cagcgccagg
    26281 tacagcagag tcagaccaac acccgccatc aggccagccc agacggtata acgggtcagc
    26341 agacgcgctt cggtaacgcc acgagaacgc gccgcgttaa caataacgat accaaacacc
    26401 attgcgccca gcgtatccat ggtcagatag ccgttaacga agccgttaga aaacgcagcg
    26461 ttttgataag cctcagtcgc cgtgctgata gaacccgccg gccagacaat cgcggcaaca
    26521 gacaggatga ccagcgcgat aattttcagc ggcgcaagga agttgcccac ggtatccagc
    26581 agcttgcccg gatagagcga aaccagaata acgatagcga aatagaccag gctgtagata
    26641 aacagcggca gcgcggaatc accagtcagc ggcgcaatcc ccacttcaaa ggaaacggta
    26701 gctgtacgcg gcgtagcgaa aagcggcccc accgccaggt aacaaaccgt tgccagcagc
    26761 acgccagcga ctttaccgat tggcgtgctg aggctgtcaa caccgccgcc aacttttgcc
    26821 agcgccacta ccgttaatac cggcaggcca acggcagtaa tgaggaagcc gaatgccgca
    26881 gtccagacgt gttcgccagc ctgcaagccg accattggag ggaaaataat gttacctgcg
    26941 ccgacgaaca acgcaaatgt cataaagccc agagcgatga tatcgcgcga tcttaattga
    27001 tgggtcataa atcatactgc ctgtggatgt ggtgttgtaa cgttaaattt ttgtgccaat
    27061 ccctggggat gatacagcgg tatatttaaa caattttagc gggaaatact cccaaccagg
    27121 cgcggcgaag aatagttttt cgatttaccg cgttaatgca gtcagtatga cagcacctga
    27181 agcgcaattt aaacgcttat aacgttttaa agcaaggagg gatctaaaaa ccagaggtat
    27241 atagcgataa gaaatcacca accagtataa aacactattt ccgtgaaaaa gacatggcta
    27301 atcatgcgaa caaaaaatca ccaaccgttg taaaatctaa cgcccgtatt ttgactgcaa
    27361 agaaaataag ccagcatcga ctggcttatg gcaataaaag atgacaaagg cggattaatc
    27421 gctgtttttg gcaattaaac gttccgggat aacaaaactg aaacgtgttc cttttcctac
    27481 tgtactctca atattcaggc gactttcgtg atgattcaca gcatgtttca cgatcgctaa
    27541 ccctaatccg ctaccgccgg tttgccggga acgcgcttta tcaacgcgat aaaaacgctc
    27601 ggtcaggcgc ggaatatgct ccggtgcaat gcccggtccg ttatcttcaa cgctaaattc
    27661 ggcaccgtgc ggcactcgct gccagcgtac ggtgatatgc gtgccttctg gagtatgatt
    27721 cacggcgtta tagaccaggt tcgaaatcgc actgcgtagc tgatcttcgt tgccagacac
    27781 tttgaggccg ttatctatct caaaggtaaa tgtctgtttt ttctgactca gagtctgagc
    27841 ctcgcgctca acaacgcgca gcatcatcgg cacatcaacc ttttcattga gcaattgcgt
    27901 cggcgcggct tcaattttcg acagcgttag caactgcttc accagccctt ccatccgctg
    27961 ggtctgctcg cgcatggtgt gcaacgcttt ttcgcgtacc gcgccttcca gcggctgctc
    28021 atccatcatc tccaggtaac cctgtaacac ggtcaatggc gtacgtaact catggctcac
    28081 gttggcaaaa aagttacgcc gcgccccttc cagttgatgc atttgcgtga catcacgcgc
    28141 caccatcagc aactgtttgt gggtataagg catgacgcga atttccagat gccgcccggt
    28201 gttgagcacc agattgagcg ggcgagaaaa atcacgcgtt ttcagatatt gcgtaaactc
    28261 cgggtaacgc agtaggttaa ggatgttctg cccgttatct tccggccagc gcaaaccaag
    28321 aatttgttgc gccagaccgt tacaccagaa aataccgctc tcttccgtgg tcagcaccac
    28381 cgcgtcgggc agcgactccg cgccgctacg aaagcgttta atcagattgc ccagttcacg
    28441 gcggcgtttt ttatttcgca gctgcatctg gtgtaagccg tatagtagcg gttcccagct
    28501 accacgcccc ggtggcgggg tcatactgcg atccacccac agccaccatg aaaggcgcaa
    28561 taaattccag aaatgccaga taagcagtcc tgttaccgat gccagcaaaa accagggcag
    28621 gtaaccaaaa aatgcaccca ggatgaaagc cgggaggcag caaagtagca gctccagcac
    28681 cagccttttc cacgacagcc gttccagcac gtaagatact cccgttaaga aatcataagc
    28741 cctgctctgc gtccgatgag caaggcgtta aaagcgggtt gaaaaacgat atcctgtacc
    28801 gcgcacggtc tgcaccatgc ggtcatgccc gccgggctcc agtgctttac gcaggcgacg
    28861 aatgtggaca tcgaccgtgc ggtcttccac atacacgtta gttccccaga cgtggtttaa
    28921 cagctgctcg cggctgtaca cgcgctcagg atgcgtcata aaaaagtgca gcagtttaaa
    28981 ttctgtcggc cccatctcca gcggctcttc gcccgccatc actcggtgag atgtcgggtc
    29041 gagacttaat ccctgcatct caatcacctc ttccaccgcc attggcgaaa tacggcgcat
    29101 taccgctttg attcgcgcca ccagctcctt cggcgaaaac ggcttggtga tatagtcatc
    29161 cgcgccggtt tcaaggccgc gcacgcgatc ttcttcttcc cctctggcgg tcaacatcac
    29221 cactggaata tcccgggtca tcgactcgcg cttaaggtgt ttgatgaact ggataccgga
    29281 gccgccaggt aacatccagt caaggagaat taaatccggc cagggttcat tcagttgatt
    29341 cacagcactg tcataatctt ccgcttcgac cggctgaaag ccattttgtt cgagcacgaa
    29401 gcagaccatt tcacgaattg gagcttcatc ttctacgacc agaatacgtc tcgccatgat
    29461 ttgccctgtt gtaataaata ggttgcgatc attaatgcga cgtcattatg cgtcagattt
    29521 atgacagatt tatgaaaagc tcgtcgcaca tatcttcagg ttattgattt ccgtggcgca
    29581 gaaaaaagca aatggcacat ctgtttgggt ataatcgcgc ccatgctttt tcgcctggga
    29641 accgttatgc gcatccttca cacctcagac tggcatctcg gccagaactt ctacagtaaa
    29701 agccgcgaag ctgaacatca ggcttttctt gactggctgc tggagacaac acaaacccat
    29761 caggtggatg cgattattgt tgccggtgat gttttcgata ccggctcgcc gcccagttac
    29821 gcccgcacgt tatacaaccg ttttgttgtc aatttacagc aaactggctg tcatctggtg
    29881 gtactggcag gaaaccatga ctcggtcgcc acgctgaatg aatcgcgcga tatcatggcg
    29941 ttcctcaata ctaccgtggt cgccagcgcc ggacatgcgc cgcaaatctt gcctcgtcgc
    30001 gacgggacgc caggcgcagt gctgtgcccc attccgtttt tacgtccgcg tgacattatt
    30061 accagccagg cggggcttaa cggtattgaa aaacagcagc atttactggc agcgattacc
    30121 gattattacc aacaacacta tgccgatgcc tgcaaactgc gcggcgatca gcctctgccc
    30181 atcatcgcca cgggacattt aacgaccgtg ggggccagta aaagtgacgc tgtgcgtgac
    30241 atttatattg gcacgctgga cgcgtttccg gcacaaaact ttccaccagc cgactacatc
    30301 gcgctcgggc atattcaccg cgcacagatt attggcggca tggaacatgt tcgctattgc
    30361 ggctccccca ttccactgag ttttgatgaa tgcggtaaga gtaaatatgt ccatctggtg
    30421 acattttcaa acggcaaatt agagagcgtg gaaaacctga acgtaccggt aacgcaaccc
    30481 atggcagtgc tgaaaggcga tctggcgtcg attaccgcac agctggaaca gtggcgcgat
    30541 gtatcgcagg agccaccagt ctggctggat atcgaaatca ctactgatga gtatctgcat
    30601 gatattcagc gcaaaatcca ggcattaacc gaatcattgc ctgtcgaagt attgctggta
    30661 cgtcggagtc gtgaacagcg cgagcgtgtg ttagccagcc aacagcgtga aaccctcagc
    30721 gaactcagcg tcgaagaggt gttcaatcgc cgtctggcac tggaagaact ggatgaatcg
    30781 cagcagcaac gtctgcagca tcttttcacc acgacgttgc ataccctcgc cggagaacac
    30841 gaagcatgaa aattctcagc ctgcgcctga aaaacctgaa ctcattaaaa ggcgaatgga
    30901 agattgattt cacccgcgag ccgttcgcca gcaacgggct gtttgctatt accggcccaa
    30961 caggtgcggg gaaaaccacc ctgctggacg ccatttgtct ggcgctgtat cacgaaactc
    31021 cgcgtctctc taacgtttca caatcgcaaa atgatctcat gacccgcgat accgccgaat
    31081 gtctggcgga ggtggagttt gaagtgaaag gtgaagcgta ccgtgcattc tggagccaga
    31141 atcgggcgcg taaccaaccc gacggtaatt tgcaggtgcc acgcgtagag ctggcgcgct
    31201 gcgccgacgg caaaattctc gccgacaaag tgaaagataa gctggaactg acagcgacgt
    31261 taaccgggct ggattacggg cgcttcactc gttcgatgct gctttcgcag gggcaatttg
    31321 ctgccttcct gaatgccaaa cccaaagaac gcgcggaatt gctcgaggag ttaaccggca
    31381 ctgaaatcta cgggcaaatc tcggcgatgg tttttgagca gcacaaatcg gcccgcacag
    31441 agctggagaa gctgcaagcg caggccagcg gcgtcacgtt gctcacgccg gaacaagtgc
    31501 aatcgctgac agcgagtttg caggtactta ctgacgaaga aaaacagtta attaccgcgc
    31561 agcagcaaga acaacaatcg ctaaactggt taacgcgtca ggacgaattg cagcaagaag
    31621 ccagccgccg tcagcaggcc ttgcaacagg cgttagccga agaagaaaaa gcgcaacctc
    31681 aactggcggc gcttagtctg gcacaaccgg cacgaaatct tcgtccacac tgggaacgca
    31741 tcgcagaaca cagcgcggcg ctggcgcata ttcgccagca gattgaagaa gtaaatactc
    31801 gcttacagag cacaatggcg cttcgcgcga gcattcgcca ccacgcggcg aagcagtcag
    31861 cagaattaca gcagcagcaa caaagcctga atacctggtt acaggaacac gaccgcttcc
    31921 gtcagtggaa caacgaactg gcgggttggc gtgcgcagtt ctcccaacaa accagcgatc
    31981 gcgagcatct gcggcaatgg cagcaacagt taacccatgc tgagcaaaaa cttaatgcgc
    32041 ttgcggcgat cacgttgacg ttaaccgccg atgaagttgc tagcgccctg gcgcaacatt
    32101 ccgagcaacg cccactgcgt cagcgcctgg tcgcgctgca tgggcagatt gttccccaac
    32161 aaaaacgtct ggcgcagtta atggtcacta tccagaatgt cactctggaa cagacgcaac
    32221 gtaatgtcgc acttaacgaa atgcgccagc gttataaaga aaagacgcag caacttgccg
    32281 atgtgaaaac catttgcgag caggaagcgc gcatcaaaac gctggaagct caacgtgcac
    32341 agttacaggc gggtcaacct tgtccacttt gtggttccac cagccacccg gcggtcgagg
    32401 cgtatcaggc gctggagcct ggtgttaatc agtctcgatt actggcgctg gaaaacgaag
    32461 ttaaaaagct cggcgaagaa ggtgcggcgc tgcgcgggca actggatgca ttaacgaagc
    32521 agcttcagcg cgatgaaaac gaagcgcaaa gcctccgaca agatgagcaa gcacttactc
    32581 aacaatggca agccgtcacg accagcctca atatcacctt gcagccacag gacgatattc
    32641 aaccgtggct ggatgcacaa gatgagcacg aacgccagct gcggttactc agccaacgac
    32701 atgaattaca agggcagatt gccgcgcata atcagcaaat tatccagtat caacagcaaa
    32761 ttgaacaacg ccagcagcag ctcttaacgg cattgacggg ttatgcactg acattgccac
    32821 aggaagatga agaagagagc tggttggcga cacgtcagca agaagcgcag agctggcagc
    32881 aacgccagaa cgaattaacc gcgctgcaaa accgtattca gcagctgacg ccgattctgg
    32941 aaacgttgcc gcaaagtgat gatctcccgc acagcgaaga aactgtggcg ctggataact
    33001 ggcggcaggt tcatgaacaa tgtctcgcat tacacagcca gcagcagacg ttacagcaac
    33061 aggatgttct ggcggcgcaa agtctgcaaa aagcccaggc gcagtttgac accgcgctac
    33121 aggccagcgt ctttgacgat cagcaggcgt tccttgcggc gctaatggat gaacaaacac
    33181 taacgcagct ggaacagctc aagcagaatc tggaaaacca gcgccgtcag gcgcaaacgc
    33241 tggtcacaca gacagcagaa acgctggcac agcatcaaca acaccgacct gacgggttgg
    33301 ctctcactgt gacggtggag cagattcagc aagagttagc gcaaactcac caaaagttgc
    33361 gtgaaaacac cacgagtcaa ggcgagattc gccagcagct gaagcaggat gcagataacc
    33421 gtcagcaaca acaaacctta ctgcagcaaa ttgctcaaat gacgcagcag gttgaggact
    33481 ggggatatct gaattcgcta ataggttcca aagagggcga taaattccgc aagtttgccc
    33541 aggggctgac gctggataat ttagtccatc tcgctaatca gcaacttacc cggctgcacg
    33601 ggcgctatct gttacagcgc aaagccagcg aggcgctgga agtcgaggtt gttgatacct
    33661 ggcaggcaga tgcagtacgc gatacccgta ccctttccgg cggcgaaagt ttcctcgtta
    33721 gtctggcgct ggcgctggcg ctttcggatc tggtcagcca taaaacacgt attgactcgc
    33781 tgttccttga tgaaggtttt ggcacgctgg atagcgaaac gctggatacc gcccttgatg
    33841 cgctggatgc cctgaacgcc agtggcaaaa ccatcggtgt gattagccac gttgaagcga
    33901 tgaaagagcg tattcctgtg cagatcaaag tgaagaagat caacggcctg ggctacagca
    33961 aactggaaag tacgtttgca gtgaaataac tattcagcag gataatgaat acagaggggc
    34021 gaattatctc ttggccttgc tggtcgttat cctgcaagct atcactttat tggctacggt
    34081 gattggtagc cgttctggtg gttgtgatgg tggtatgaaa aaagtcattt tatctttggc
    34141 tctgggcacg tttggtttgg ggatggccga atttggcatt atgggcgtgc tcacggagct
    34201 ggcgcataac gtaggaattt cgattcctgc tgccgggcat atgatctcgt attatgcact
    34261 gggggtggtg gtcggtgcgc caatcatcgc actcttttcc agccgctact cactcaaaca
    34321 tatcttgttg tttctggtgg cgttgtgcgt cattggcaac gccatgttca cgctctcttc
    34381 gtcttacctg atgcttgcca ttggtcgcct ggtttccggc tttccgcatg gcgcattttt
    34441 tggcgtcggc gcgatcgtgt tatcaaaaat tatcaaaccc ggaaaagtca ccgccgccgt
    34501 ggcggggatg gtttccggga tgacagtcgc caatttgctg ggcattccgc tgggaacgta
    34561 tttaagtcag gaatttagct ggcgttacac ctttttattg atcgctgttt ttaatattgc
    34621 ggtgatggca tcggtctatt tttgggtgcc agatattcgc gacgaggcga aaggaaatct
    34681 gcgcgaacaa tttcactttt tgcgcagccc ggccccgtgg ttaattttcg ccgccacgat
    34741 gtttggcaac gcaggtgtct ttgcctggtt cagctacgta aagccataca tgatgtttat
    34801 ttccgttttt tcggaaacgg cgatgacctt tattatgatg ttagttgggc tagggatggt
    34861 gctgggaaat atgctaagtg gcaggatttc aggacgttat tcaccactgc gcattgcagc
    34921 agtgactgac tttatcattg tactggcact gctgatgctc tttttctgcg gcggcatgaa
    34981 aacaacgtcg cttatttttg cttttatttg ttgcgcggga ttatttgccc tttcagcacc
    35041 tctgcaaata ttgttactgc aaaacgccaa aggcggagag ttattaggtg ccgcaggtgg
    35101 gcaaatagcg tttaacctcg gtagcgccgt cggcgcatat tgcggaggta tgatgctgac
    35161 gctggggctg gcatataatt acgtggcgct gcctgccgcc ctgctttcgt ttgctgcgat
    35221 gtcatcgttg ctgctgtatg gtcgctataa gcgccagcaa gcggcggata gtccggtgct
    35281 ggcgaaacca ctggggtaga ttactgtcac ggtgcgcgtt gcttattgcc ggatgcggcg
    35341 tgaacgcctt atccgcccta cgcggttctg gcacattttg taggcctgat aagacgcggc
    35401 aagcgtcgca tcaggcatga tgcgccaatt gcctacgttt tttactcttg tggccataac
    35461 cacgcagcgc cgcgtacgcc gctggaatca ccgtgcttcg ccttacgcac cggcgtttca
    35521 cattcgccgc cgaagacaaa ttgtttaatc aactgcccaa ccgtttgata taaacggtct
    35581 acattgctca tcccgccccc caggacaatc acatccggat cgagaatatt cacgacatgt
    35641 gccagcgatt ttgccagccg cagctcgtag cgacgcaatg ccagttccgc taccggatcg
    35701 ctttcttcaa ccaggcggat aatttcactg cctttcagcg catgtccgct caaacgacga
    35761 taatccgtcg cgaatcccgt gcccgaaata aaggtttcaa tacaaccttg tttaccgcaa
    35821 taacaaggga cttcctcgcg ataacgcagc tcgtcttcat ccatccacgg tagcggattg
    35881 tgtccccact cgcctgccgt gccattgccg ccgatatgcg cccgcccatt taatgccacg
    35941 cccgcgccgc atcccgtgcc gataatcacg gcaaataccg tctgcgctcc cgctgccgcg
    36001 ccatctactg cttctgaaac cgccagacag ttggcatcat ttgccagccg cacttcccgc
    36061 tgcaacctcg cgcttaagtc tttatcgaat ggctgaccgt tgagccaggt tgaattggca
    36121 ttcttcacca caccggtgta gggcgaaatc gaacctggaa tgcccatacc taccgttccg
    36181 cgctgccctg tcgcctgctc cgccatatca accaacgtgg cgatcgtttc aatagtctgc
    36241 cggtaatcat cacgcggcgt gggcagacga tggcggtaca actgctcccc tgcatcgccc
    36301 agtgcaatca cttcagtttt ggtgccgcct aaatcgatac ctatacgcac tgtactctcc
    36361 ttattttttt caatatcaat agcgtagaga cggacaaccg gattggcaat gcaaggccgc
    36421 cgacaattcg ttatcatgcc cgctaaattt aacgacaagg ccgtggaaat tatcatgctg
    36481 tggttcaaaa atttaatggt ttaccgtctt agccgcgaga tttcgctgcg tgcagaagag
    36541 atggaaaaac agctagcctc gatggcattt accccatgcg gcagccagga catggcgaag
    36601 atgggctggg ttcctccgat gggatcgcac agcgatgcgt taacgcacgt tgccaatggt
    36661 caaattgtta tctgcgcgcg caaagaagaa aaaatcctcc cgtctccggt gattaaacag
    36721 gcgctggaag cgaaaatcgc caaactggaa gcggaacagg cgcgtaagct gaagaaaacc
    36781 gaaaaagatt cgctgaaaga cgaagtgctg cactctctgc tgccgcgtgc tttcagccgt
    36841 tttagccaga caatgatgtg gatcgacacg gttaacggtt tgattatggt ggactgcgcc
    36901 agtgccaaaa aagcggaaga tacgctggca ttactgcgta aaagcctggg gtcgttaccg
    36961 gttgtaccat tgagcatgga aaacccgatt gaactgacgc tgaccgaatg ggttcgctcc
    37021 ggtagtgcgg cacagggctt ccagctgctt gatgaggccg agctgaaatc gttgctggaa
    37081 gatggcggcg tgatccgcgc gaagaaacaa gatctgacca gcgaagagat caccaatcac
    37141 attgaagccg gaaaagtggt gactaaactg gcgctcgact ggcagcagcg cattcagttt
    37201 gtgatgtgcg acgatggttc gctcaagcgt cttaagttct gcgacgagct gcgcgatcaa
    37261 aacgaagata tcgaccgtga agatttcgcc ctgcgttttg atgccgattt catcctgatg
    37321 actggtgaac tggcagcgtt aattcaaaac ctgattgaag gattaggtgg cgaagcacaa
    37381 cgttaattgc tgattttcct ttaatgccgg atgcgacgcc tgccgcgtct tatccggcgt
    37441 acgaagccac accaggcata taattattcg ctacggcgag caataatttt tagcgcagca
    37501 atattatgcg ttttacgccg taactgatct aatcgcctca tccctattcg cctgtcagca
    37561 gcacgcagaa tattcactaa cccccattta tcgctggtaa attgttttgt taacagctcc
    37621 gcttttgggg tatcaatata ttcacgcaat aaatcagaaa ttgaacaaat atccgtcagg
    37681 taaggatacc agcaagtttc accgtgaaaa ttacgcactc cagcgttacc tgcaataaag
    37741 tctttcgggt aatcccagat cacgttttta tccaacgtga tccaataacg cggtaaatcg
    37801 gtacttccac cattttggct gcgcatgaga taacgggtac agtgaatttg caggttgatc
    37861 gttggcgtca gtaagtcata gatctcccgc tgtaacttgc tccatggatg ttgtgtcatt
    37921 gtttttcctc aagccggtga cgcgcttcac tgcgaatttt ccgcgttgcc ccggttttca
    37981 ctaaatcacg taactgcgaa agatcgggtt gttcgacaaa taagagtaat gatagatcct
    38041 cttttaacca cgccgttttc acatcggcag ccagctgtgg attattaata gccagcgacc
    38101 gatcctgaga ctcagtcaat gtcgttaaca acgacgccgg ggtatgtgcg ttgcgtaatg
    38161 ccgtttggcg caaatgatca ttgcgacttt gcagcaatgc acgccaccat tcatcatctg
    38221 atttatcttt tatcgtagcc agttccgcaa aaaatacctg gagatcttca ctgatattta
    38281 tatcaacctt gatgaattat gaatatatcg gaaattgaaa aaatcgcagc aaagga
//
LOCUS       DAZOYL010000006        38226 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZOYL010000006 DAZOYL010000000
VERSION     DAZOYL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38226
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.006"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            101..175
                     /locus_tag="ACHUOY_000211"
     tRNA            101..175
                     /locus_tag="ACHUOY_000211"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:133..135,aa:Gln,seq:ctg)
     gene            complement(329..1504)
                     /gene="ubiF"
                     /locus_tag="ACHUOY_000212"
     CDS             complement(329..1504)
                     /gene="ubiF"
                     /locus_tag="ACHUOY_000212"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR0681111.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1650..3074
                     /gene="miaB"
                     /locus_tag="ACHUOY_000213"
     CDS             1650..3074
                     /gene="miaB"
                     /locus_tag="ACHUOY_000213"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR0681112.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3227..4267
                     /locus_tag="ACHUOY_000214"
     CDS             3227..4267
                     /locus_tag="ACHUOY_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR0681113.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4264..4731
                     /gene="ybeY"
                     /locus_tag="ACHUOY_000215"
     CDS             4264..4731
                     /gene="ybeY"
                     /locus_tag="ACHUOY_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR0681114.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4821..5699
                     /gene="corC"
                     /locus_tag="ACHUOY_000216"
     CDS             4821..5699
                     /gene="corC"
                     /locus_tag="ACHUOY_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR0681115.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5724..7262
                     /gene="lnt"
                     /locus_tag="ACHUOY_000217"
     CDS             5724..7262
                     /gene="lnt"
                     /locus_tag="ACHUOY_000217"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR0681116.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7340..9217)
                     /locus_tag="ACHUOY_000218"
     CDS             complement(7340..9217)
                     /locus_tag="ACHUOY_000218"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR0681117.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            9672..10580
                     /gene="gltI"
                     /locus_tag="ACHUOY_000219"
     CDS             9672..10580
                     /gene="gltI"
                     /locus_tag="ACHUOY_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR0681118.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10750..11490
                     /gene="gltJ"
                     /locus_tag="ACHUOY_000220"
     CDS             10750..11490
                     /gene="gltJ"
                     /locus_tag="ACHUOY_000220"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR0681119.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            11490..12164
                     /gene="gltK"
                     /locus_tag="ACHUOY_000221"
     CDS             11490..12164
                     /gene="gltK"
                     /locus_tag="ACHUOY_000221"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR0681120.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12164..12889
                     /gene="gltL"
                     /locus_tag="ACHUOY_000222"
     CDS             12164..12889
                     /gene="gltL"
                     /locus_tag="ACHUOY_000222"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR0681121.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            13007..13942
                     /gene="rihA"
                     /locus_tag="ACHUOY_000223"
     CDS             13007..13942
                     /gene="rihA"
                     /locus_tag="ACHUOY_000223"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR0681122.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            14034..15704
                     /gene="hscC"
                     /locus_tag="ACHUOY_000224"
     CDS             14034..15704
                     /gene="hscC"
                     /locus_tag="ACHUOY_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR0681123.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(15764..17218)
                     /gene="djlC"
                     /locus_tag="ACHUOY_000225"
     CDS             complement(15764..17218)
                     /gene="djlC"
                     /locus_tag="ACHUOY_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR0681124.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDDEEEREAFLEA
                     IQAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAK
                     LHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLC
                     AYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTR
                     LLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVV
                     LEAFPKQEMLARLNTRLEGGDTHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKN
                     DEDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(17215..17922)
                     /locus_tag="ACHUOY_000226"
     CDS             complement(17215..17922)
                     /locus_tag="ACHUOY_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0681125.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            18024..18578
                     /locus_tag="ACHUOY_000227"
     CDS             18024..18578
                     /locus_tag="ACHUOY_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0681126.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(18588..20015)
                     /locus_tag="ACHUOY_000228"
     CDS             complement(18588..20015)
                     /locus_tag="ACHUOY_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR0681127.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(20012..20719)
                     /locus_tag="ACHUOY_000229"
     CDS             complement(20012..20719)
                     /locus_tag="ACHUOY_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0681128.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            20883..21859
                     /gene="ybeQ"
                     /locus_tag="ACHUOY_000230"
                     /pseudo
     CDS             20883..21859
                     /gene="ybeQ"
                     /locus_tag="ACHUOY_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(21929..22411)
                     /locus_tag="ACHUOY_000231"
     CDS             complement(21929..22411)
                     /locus_tag="ACHUOY_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR0681129.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            22646..25228
                     /gene="leuS"
                     /locus_tag="ACHUOY_000232"
     CDS             22646..25228
                     /gene="leuS"
                     /locus_tag="ACHUOY_000232"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR0681130.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            25243..25824
                     /gene="lptE"
                     /locus_tag="ACHUOY_000233"
     CDS             25243..25824
                     /gene="lptE"
                     /locus_tag="ACHUOY_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR0681131.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            25824..26855
                     /gene="holA"
                     /locus_tag="ACHUOY_000234"
     CDS             25824..26855
                     /gene="holA"
                     /locus_tag="ACHUOY_000234"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR0681132.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            26857..27498
                     /gene="nadD"
                     /locus_tag="ACHUOY_000235"
     CDS             26857..27498
                     /gene="nadD"
                     /locus_tag="ACHUOY_000235"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR0681133.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            27522..28133
                     /locus_tag="ACHUOY_000236"
                     /pseudo
     CDS             27522..28133
                     /locus_tag="ACHUOY_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            28393..28710
                     /gene="rsfS"
                     /locus_tag="ACHUOY_000237"
     CDS             28393..28710
                     /gene="rsfS"
                     /locus_tag="ACHUOY_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR0681134.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            28714..29181
                     /gene="rlmH"
                     /locus_tag="ACHUOY_000238"
     CDS             28714..29181
                     /gene="rlmH"
                     /locus_tag="ACHUOY_000238"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR0681135.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            29212..31113
                     /gene="mrdA"
                     /locus_tag="ACHUOY_000239"
     CDS             29212..31113
                     /gene="mrdA"
                     /locus_tag="ACHUOY_000239"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR0681136.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            31116..32228
                     /gene="mrdB"
                     /locus_tag="ACHUOY_000240"
                     /gene_synonym="rodA"
     CDS             31116..32228
                     /gene="mrdB"
                     /locus_tag="ACHUOY_000240"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR0681137.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            32239..33327
                     /gene="rlpA"
                     /locus_tag="ACHUOY_000241"
     CDS             32239..33327
                     /gene="rlpA"
                     /locus_tag="ACHUOY_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR0681138.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            33466..34677
                     /gene="dacA"
                     /locus_tag="ACHUOY_000242"
     CDS             33466..34677
                     /gene="dacA"
                     /locus_tag="ACHUOY_000242"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR0681139.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            34787..35050
                     /gene="ybeD"
                     /locus_tag="ACHUOY_000243"
     CDS             34787..35050
                     /gene="ybeD"
                     /locus_tag="ACHUOY_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR0681140.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            35151..35792
                     /gene="lipB"
                     /locus_tag="ACHUOY_000244"
     CDS             35151..35792
                     /gene="lipB"
                     /locus_tag="ACHUOY_000244"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR0681141.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            36052..37005
                     /locus_tag="ACHUOY_000245"
     CDS             36052..37005
                     /locus_tag="ACHUOY_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR0681142.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            37214..38179
                     /gene="lipA"
                     /locus_tag="ACHUOY_000246"
     CDS             37214..38179
                     /gene="lipA"
                     /locus_tag="ACHUOY_000246"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR0681143.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         9584 a         9754 c         9587 g         9301 t
ORIGIN      
        1 cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac caaattctga
       61 atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg ccaagcggta
      121 aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccacatta
      181 aaaaagctcg cttcggcgag ctttttgctt ttctgcgttc attcaatgtc gaatgcgatg
      241 ttgacacgtc ttatccttca atgtcggatg cgacgctgcc gcgtcttatc cgacctacgg
      301 ttggcacgca tccggcaatg ttgtaaggct acaaccctaa cgcatatttc agcgcctgac
      361 gtttcaacac gccagcacgc tctgccgcca ttaacccgag attacgcaca aaacgcagcg
      421 gcggcagatt attactgaat ccggcataaa acagatccat accactttgc ataatgaagt
      481 tatccgccat gcgccgcatc tggtaacgct tgaggacagg ataactggcc cacgcttcac
      541 cgtagctgcg ggcattcacc agaacatcaa tcagggcatc gacatcacga taaccaagat
      601 tcaccccctg ccccgccagc ggatggatgg tatgcgcggc atcgcccacc agcgcaagcc
      661 ccggctgcac atactgcaac gcatgtcggc gcgtcagcgg aaacgcacca gcggcaagcg
      721 gtgtaacgta acccagacgc gacgggaaat gcttcgcgat ttccgcctgg aattgcgcca
      781 tattcatatt ctgcaactgg cgaatacgcg ccggagagtc ataccacacc agcgatgccc
      841 agttatcaaa caacggcaga aacgcacgcg gtccgtccgg ggtaaattgc tgccaggtgc
      901 tgtcgccggg atcgttctca cactgtacgc taatcaacat gcacgactgc gcatactgcc
      961 aggcatgaac gccaattccc gccatctgcc gcacctgaga atttgcgccg tcggcaccaa
     1021 tcaccagttt cgcgcggatc gtttcaccgc ccttcagttc cagctcctga agatcattat
     1081 ctcgatgtaa tgcaatcagc gagcctggca cacgtaacgt tactttcgga tgcgcttcca
     1141 gcgcctgcca cagcgcctgt tgcaggacgg tgttttccac catatagcca agcaacggta
     1201 gcttaagttc agcggcgtca aacaccacat gcgccgtttc ccactcccac gtttccagtc
     1261 tgcggtaagg atggcaacgc atagcctgta ctgcatccca gacccctaac cctttaagca
     1321 atgataccga tgcggcgctg atcgccgaga tccgcacgtc cggttggcta tcagcgacaa
     1381 acggtgcagg ttctgcgtgc tcgatcaccg ttaccgaaaa tccgtgctgt gccagcccca
     1441 gcgccagcgc gccgccgacc attcctccgc cgacaatggc aatttccgtt ggttgatttg
     1501 tcatggtcaa tcatcctgtt agcaatatgc tttaagttta caggattttt acccccaagg
     1561 gctgataacg ctcatactgg tcacaacggc agcaaagcat tacactatac gccctgcatt
     1621 cctggctact atttcgcaag agcaagtcga tgaccaaaaa actccatatt aaaacctggg
     1681 gctgtcagat gaacgagtac gattcatcga agatggccga tctgctggat gccacccacg
     1741 gctatcaact gaccgacgtg gcggaagaag cggatgtgct gctgctgaac acctgctcaa
     1801 tccgcgagaa ggctcaggaa aaagtcttcc atcagttggg tcgctggaaa ctgttaaaag
     1861 agaagaatcc agacctgatt atcggcgtcg gtggctgcgt ggcatcgcaa gaaggcgagc
     1921 acattcgcca gcgcgcccac tatgtcgata ttatttttgg gccgcaaacg ctgcaccgtc
     1981 tgccggagat gatcaactcc gtgcgcggcg accgcagccc ggttgtagat atcagcttcc
     2041 cggaaatcga gaagtttgac cgtctaccgg aaccgcgcgc cgaagggccg accgcgtttg
     2101 tctccatcat ggaaggctgc aataaatatt gcacctactg cgtggtgcct tacacccgtg
     2161 gtgaagaggt aagccgtccg tctgacgata ttctgtttga gattgcccag cttgcggctc
     2221 agggcgtgcg tgaagtcaac ctgctcggtc agaacgtgaa cgcctggcgt ggtgaaaact
     2281 acgacggctc caccggatcg tttgccgatc tgctgcgtct ggttgctgcg atcgacggga
     2341 tcgatcgtat tcgctttacc accagccatc cgatcgaatt caccgacgat atcatcgaag
     2401 tgtatcgcga cacgccggag ctggtgagct tcctgcatct gccagtacaa agcggttccg
     2461 atcgcattct gaacctgatg ggccgtactc acacggcgct ggagtacaaa gcgatcatcc
     2521 gtaaactgcg tgcggcgcgt ccggatattc agatcagttc tgacttcatt gttggcttcc
     2581 ctggcgaaac caccgaagac ttcgagaaaa cgatgaagct gattgccgac gtcaatttcg
     2641 acatgagcta cagctttatc ttctctgcac gtccgggtac accagccgcc gatatggttg
     2701 atgatgttcc ggaagaagag aagaagcaac gtctgtatat tctgcaagaa cgtatcaatc
     2761 agcaagcgat ggcatggagc cgccggatgc tcggcaccac ccagcgtatt ctggtagaag
     2821 ggacttcgcg taagagcatc atggagcttt ccgggcgtac cgaaaataac cgcgtggtca
     2881 acttcgaggg cacaccggat atgatcggta aattcgtcga tgtagaaatt accgacgtct
     2941 acccgaactc tctgcgcggt aaagtggtgc gtactgaaga tgaaatgggt ctgcgcgtgg
     3001 cagaaacacc ggaatcggtg attgcccgta cccgcaaaga aaacgacctt ggcgtgggtt
     3061 attatcagcc gtaattctca ggcccgccgt tccggtgggc cttgtttttt ctctctttat
     3121 ccccatcttt atcgcaatgc ttgcgccttt ttcctgtggc gtgaataatt tccttatagg
     3181 ccggtgaatg ttcagcttcc ggcggcatac agataagagg aacggtttga acatagacac
     3241 tcgcgaaatc accctggagc cagcagacaa cgcgcgtctg ttgagcctgt gcggcccgtt
     3301 tgatgacaac atcaagcagc tcgaacgccg tctcggcatc gagatcaatc gccgcgacaa
     3361 ccactttaaa ctgaccggcc gtccgatttg cgtcaccgct gcggcagaca ttctgcgtag
     3421 cctgtatgtc gatacagccc cgatgcgcgg tcagattcag gatatcgaac cggaacagat
     3481 ccaccttgcg attaaagaag cgcgagtact ggagcaaagt gcggagagcg tgccggagta
     3541 cggcaaagcg gtcaatatca aaaccaaacg cggcgtaatt aagccgcgca cgccaaacca
     3601 ggcgcagtac atcgccaata ttctcgacca tgacattacc ttcggcgttg gcccggcggg
     3661 tacgggtaaa acctacctgg cagtggctgc ggcagttgat gccctggagc gtcaggagat
     3721 tcgccgtatt ctgctgactc gtccggcggt cgaagccggt gagaaactgg gcttcctgcc
     3781 tggcgattta agccagaaag tagacccgta tctgcgccca ctgtacgacg cgctgtttga
     3841 aatgctgggc tttgagaaag ttgagaaact gattgagcgc aacgttattg aagtcgcgcc
     3901 gctggcctat atgcgtggtc gtacgctgaa cgacgcgttt atcattctcg atgagagcca
     3961 gaacaccacc atcgaacaga tgaagatgtt cctgacccgt atcggtttta actcaaaagc
     4021 ggttatcacc ggcgacgtca cacagatcga cctgccgcgt aatactaaat caggcttacg
     4081 tcacgccatc gaagtgctgg ccgatgtcga agagatcagc tttaacttct tccacagcga
     4141 agacgtggtt cgtcaccctg tggtggcgcg tatcgttaac gcctatgaag cctgggaaga
     4201 agccgaacaa aaacgaaaag cggcgctggc ggcagaacgc aagcgcgaag aacaggaaca
     4261 aaaatgagtc aggtgatcct cgatttacaa ctggcatgtg aagataattc cgggttaccg
     4321 gaagagagcc agtttcaaac atggctgaat gcggtgatcc cgcagtttca ggaagaatcg
     4381 gaagtgacta ttcgcgtggt cgataccgcc gaaagccaca gtctgaatct gacctatcgc
     4441 ggtaaggata agccgaccaa cgtgctctcc ttcccgtttg aagtgccgcc tggcatggaa
     4501 atgtcgctac tgggcgatct ggttatctgc cgtcaggtgg ttgagaagga agctcaggag
     4561 caaggcaaac cactggaggc gcactgggcg catatggtgg tgcacggcag tctgcatttg
     4621 ttaggttacg atcacatcga agatgacgaa gcagaagaaa tggaagccct cgaaacagag
     4681 attatgcttg ctctgggcta tgaggatccg tacattgccg agaaagaata atttgccagg
     4741 catttgactg gcagacgtcc ccgccgttgg tgattaacga cgacgttttg attaactaac
     4801 atgagaaccc atacgacgcc atgagcgacg acaattcaca cagtagtgac acgataagca
     4861 acaagaaggg atttttctcc ctgttactca gccaactttt ccacggtgaa ccgaaaaacc
     4921 gtgacgaact gctggcgctg atccgtgatt ccgggcagaa cgaccttatc gacgaagata
     4981 cgcgcgatat gctcgaaggg gtgatggaca tcgcagacca acgcgttcgc gacatcatga
     5041 tcccccgctc ccagatgatt accctgaaac gcaaccagac gctggacgaa tgccttgatg
     5101 tcatcatcga gtccgcccac tcacgtttcc cggtgattag cgaagacaaa gatcacattg
     5161 aagggattct gatggcgaaa gacttgctgc cgtttatgcg cagcgatgct gaagccttca
     5221 gcatggacaa agtgttacgt caggcggttg tcgttcctga aagtaagcgc gtagaccgga
     5281 tgctgaaaga gtttcgctct cagcgttacc acatggcgat cgtcattgac gaattcggtg
     5341 gggtttccgg tctggtgacc attgaagaca tcctggaact gattgttggt gagattgaag
     5401 acgagtatga cgaagaagat gatatcgact tccgtcagct gagtcgtcat acctggaccg
     5461 tgcgtgcact ggcttccatt gaagacttca acgaagcgtt cggcacccac tttagcgatg
     5521 aagaggttga cactatcggt ggtctggtga tgcaggcatt tgggcatctt ccggcgcgtg
     5581 gcgaaactat cgacatcgac ggttaccagt tcaaagtggc gatggccgac agtcggcgta
     5641 ttattcaggt tcatgtcaaa atcccggatg actcacccca gccgaagctg gatgaataaa
     5701 accgaaactg gatagataac tacatggctt ttgcctcatt aattgaacgc cagcgcattc
     5761 gcctgctgct ggcgttatta ttcggtgcct gcggaacgct ggccttctct ccttacgacg
     5821 tctggcctgc ggcgattatt tcgttgatgg ggcttcaggc gttgaccttt aaccgccgtc
     5881 cactccagtc tgccgctatt ggcttttgct ggggatttgg cctctttggc agcggtatta
     5941 actgggtcta tgtcagcatc gcgacctttg gcggaatgcc tggcccggtt aacatcttcc
     6001 tggtggtgct gctggcggcg tatttgtcgc tgtataccgg actgtttgct ggcgtgctgt
     6061 cgcgtctgtg gccgaaaacc acctggctgc gcgtggcgat tgccgcccct gctctctggc
     6121 aagtgaccga gtttctgcgc ggttgggtac tgacaggctt cccgtggtta cagttcggct
     6181 atagccagat tgatggcccg ttaaaagggc tggcaccgat aatgggcgtg gaagccatta
     6241 acttcctgct gatgatggtt agtggcctgc tggcactggc gttggtcaaa cgcaactggc
     6301 gtccgctggt ggtggccgtc gtgctgtttg ccctaccctt cccgctgcgt tacatccaat
     6361 ggtttacccc gcaaccggag aaaaccattc aggtttcgat ggttcagggc gatattccgc
     6421 aatcactgaa atgggatgga gatcagcttc ttaatacgct gaagatttac tacaacgcaa
     6481 cggctccgct gatgggcaaa tcatcgttga ttatctggcc ggagtcggcg ataaccgatc
     6541 tggaaattaa tcagcaaccg ttcctcaaag cactggacgg tgagttgcgc gataaaggta
     6601 gctcgctggt aaccgggatt gtcgacgcgc gtctcaataa gcagaaccgc tacgatacct
     6661 acaacaccat catcacactg ggtaaaggtg caccatacag ctacgaatca gccgatcgct
     6721 ataacaaaaa ccatctagtg ccgtttggcg agtttgtccc gctggagtcg attctgcgtc
     6781 cgttagcacc gttctttgat ctgccgatgt cgtcgttcag ccgtgggcca tatatccagc
     6841 cgccgctgtc ggtaaatggt attgagctta ctgcggctat ttgctacgag atcattctcg
     6901 gcgagcaagt gcgcgataac ttccgcccgg ataccgacta tctgctgact atctccaacg
     6961 atgcgtggtt tggtaagtct attggtccat ggcagcactt ccagatggcg cgaatgcgtg
     7021 cgctggagct ggcgcgccca ctgttgcgca gcaccaacaa cggcattacg gcggtgattg
     7081 gcccgcaggg ggaggttcag gcgatgatcc cgcagttcac ccgcgaggtg ttgaccacta
     7141 atgtgacacc gaccaccgga ctcacaccgt atgcacgtac cggcaactgg ccactgtggg
     7201 tgctgacggc gctgtttggt tttgccgctg tgttgatgag tctgcgagca cgtaaacgtt
     7261 gatgtgcctg atgtatcctg catgagcaaa acgtactttc tcattgagct ttggggcatg
     7321 gttatcatgc ccctttttct tacggttcag ggatacaagc cacattgcca tattggggat
     7381 gacgaataat tgcctgcgga tcaagcgagc gcgtcctgtc catttggatc aacccgttct
     7441 tcgccatgac aaatattttt ctaccatccg ctgaaaaacg aaggtgatta acgtagctgg
     7501 caggataaac gccaataatc tgcccggcat taatattgag caggatgaca taaccttgca
     7561 ggccatatga caccaacgct aaatattgac cgtccgggct aataccctca acggaataaa
     7621 cgctcctgct acaccattgc gtcataatgc tggcttgttg atcccctacg cttatacgtg
     7681 aaatcccctg actatcggca aaaataacct cgccgttttc aggtcgaaaa cgcatcgccg
     7741 atatcgttcc acgcgcatca cctgcgggaa agtcttcttt ctgatgcgtg atggtattga
     7801 tccgcacgat gttacctctg cctgctcccg gaaccagtaa tgttctttca tccggggaaa
     7861 gtgccataac ccaggcgcct ggtttaccgc tcgtccccgt cccgtcgtat ttctccagcg
     7921 ggatatccgc cacatgctga taagtcacca gatcaatcgc tggcacacta ttactggtgc
     7981 gattcatcac ccaggctttt gtgccatcac gcgagaggat aaggctgtca ggatgtgcac
     8041 cggtggtaat cgactgaata atctcccctg tcgccacgtt aatgaccgat aacgcattcg
     8101 cttcaaatga agtcgcataa atcaactttt catccgggct taatgccagc gatctgacgc
     8161 cctgatcttt acagccacca caatgtttga ctgacttttc tttcgctatg ggcgctgtaa
     8221 aggtatgcag gattttcttt tgtccgaggt cgaaaacaac aatgctgttc gtgttttcaa
     8281 ttgcggcata caacatatta ccatcccgac tgatcgccat atcatggata tcggcaatct
     8341 cagcgagcaa atcaccactg gcctgttcac gacttaccgg agcggataat gtttccctga
     8401 gggcgttgcg ttcctccgct aactgctgct ttttttgccg tcgctcttgt tcaatctcct
     8461 gtggataaaa cgcttcacgc aggctttgcc tgacctgtaa aactgactca tccatctgct
     8521 gctgcgcaag ccagatagcc ccggtcagaa gaagactgcc cgcaacaata atcccgcctt
     8581 ccgtaacgag tcggttttgc ccagataaac gcgcgctcag ccaacccaac gcgccacccg
     8641 caatcaggcc gccaatgtgg caagcgttat ctattcctgt ttgccgggca ccgtacaaca
     8701 gtgtcagcgc caccataccc aacagaggaa atactcgccg ctggttttta tggtatgtgc
     8761 ccgtacctga agcaaactgt gttgctatcg ctgcaccagc tattcccaga atcgccccgg
     8821 atgccccaac gctgatgact atctgctcac tttcgcgtaa cgcataacag gcgctgatga
     8881 gcccaccaaa gacgccggag aataaccagg taatcaggag tcgccatttc cccagaatct
     8941 gttccgccac gcctcccacg accaggaatg caaacatatt catcagcaga tgagaaacac
     9001 tactgtgcag aaagatactg ctcaataagc gccagggttc acccgacaaa gtaagaggcg
     9061 caacgtttgc gccgagatgg ataagtgcgg attgatcgac ctcttcataa ctcatccatt
     9121 gtgcgagata acaaaatatc ccaatacaga gtgcaaaaag gataagggtt attgcgggta
     9181 attgaacatt taacggctta accgaagatg cagacataag gtgtttcttc cttgaaaaga
     9241 caggtaaaaa gcatttttcc cgtgtgcagt tatcatcccg gcaaacgtcc catgccgaaa
     9301 agataattct gtccgaaata tgactttttc agcctaaaat tacaatcgta attaattcgc
     9361 ccctaagtat tttttggcat gtaagttgct ttatttactg tgtaagcgat gaaatcagcc
     9421 gcgacgcaac caggttgcac caccacaaat cagcggtagc accaaaaagg tgcggtttaa
     9481 gaattttgct tcggaatggt gcggcgcatc tcgcaaaaac aaacaataaa acagcaatat
     9541 ctttacattt agccaaacta aatgttacct aacaggcaca acactgcaca ataaagttgc
     9601 agacgataac aacacaaaca ctcacaacgg gtatccatgc gttcttaacg cagaagataa
     9661 aggagttgga tatgcaatta cgtaaacctg ccacagcaat cctcgccctg gcgctttccg
     9721 caggactggc acaggcagat gacgccgccc cggcagcagg cagtacgctg gacaaaatcg
     9781 ccaaaaacgg tgtgattgtc gtcggtcacc gtgaatcttc agtacctttc tcttattacg
     9841 acaatcagca aaaagtggtg ggttactcgc aggattactc caacgccatt gttgaagcag
     9901 tgaaaaagaa actcaacaaa ccggacttgc aggtaaaact gattccgatt acctcacaaa
     9961 accgtattcc actgctgcaa aacggcactt tcgattttga atgtggttct accaccaaca
    10021 acgtcgaacg ccaaaaacag gcggctttct ctgatactat tttcgtggtc ggtacgcgcc
    10081 tgctgaccaa aaagggcggc gatatcaaag attttgccga cctgaaagac aaagccgtag
    10141 tcgtcacttc cggcactacc tctgaagttt tgctcaacaa actgaatgaa gagcaaaaaa
    10201 tgaatatgcg catcatcagc gccaaagatc acagtgactc tttccgcacc ctggaaagcg
    10261 gtcgtgccgt tgcctttatg atggatgatg ccctgctggc cggtgaacgt gcgaaagcga
    10321 agaaaccaga caactgggaa atcgtcggca agccgcagtc tcaggaagcc tacggttgca
    10381 tgttgcgtaa agacgatccg cagttcaaaa agctgatgga tgacaccacc gcgcaggtgc
    10441 agacctccgg tgaagcggaa aaatggtttg ataaatggtt caaaaatccc attccgccga
    10501 aaaacctgaa catgaatttc gaactgtcag acgaaatgaa agcactgttc aaagaaccga
    10561 atgacaaggc actgaactaa ttacaagaac caggggcgga aattccagcc ctctcgattg
    10621 ttacgtagca cggacagact atacgcctga tggtcgttcc ccatcgggcc tgaaaaccgc
    10681 aatacgctgg gtaacaatct tcgagggtag cagttaacgc tgctaccctc ttttttctgg
    10741 agtagattta tgtctataga ctggaactgg ggtatttttt tacaacaagc cccgttcggc
    10801 aacaccacct atcttggttg gatctggagt ggttttcagg tgacgatcgc tttgtcgatc
    10861 tgcgcctgga ttatcgcttt cctcgtcggt tcatttttcg gcattttacg taccgttcca
    10921 aaccgttttc tctccggtct gggtacgttg tatgtcgaac tgttccgcaa cgtgccgttg
    10981 attgtgcaat tctttacctg gtatctggtg atcccggagc tgttgccgga gaaaatcggc
    11041 atgtggttta aggccgagct ggatcccaat attcagtttt tcctttcatc catgctctgc
    11101 ctggggctgt ttaccgccgc ccgtgtttgc gaacaggtac gcgccgccat tcagtcgttg
    11161 ccgcgtgggc aaaaaaatgc cgcgctggcg atgggcctga cgctaccgca ggcttatcga
    11221 tacgtactgc tgcctaatgc ttatcgcgtt atcgtcccgc cgatgacttc cgagatgatg
    11281 aacctggtga aaaactccgc catcgcctcg actattggtc tggtggatat ggcggcgcag
    11341 gcgggtaaat tgcttgatta ctcagcccac gcctgggagt cgtttaccgc tattacgctg
    11401 gcgtatgtgt tgattaacgc ggttatcatg ctggtaatga cgctggttga acgtaaagtt
    11461 cgcctgcctg gcaacatggg gggcaaataa tgtacgagtt tgactggagt tccattgtcc
    11521 cttccctgcc atatctgctc gacgggctgg tgatcacgct gaaaatcacc gtcacggcag
    11581 tcgtgattgg tattttgtgg ggcaccatgc tggcggtgat gcgcttgtcc agctttgcgc
    11641 ccgtagcgtg gtttgccaaa gcctacgtta acgtattccg ctcgattcct ttagttatgg
    11701 ttttgctgtg gttttacctg atcgtgccag gttttctgca aaacgtgctg ggattatcac
    11761 caaaaaatga tatccgttta atttcggcga tggtggcgtt ttcgatgttt gaagcggcct
    11821 actattcaga gattatccgt gctggtattc aaagtatttc tcgtggtcag tcgagcgccg
    11881 cgctggcgtt gggaatgact cactggcagt cgatgaaact gattattctg ccgcaggcgt
    11941 tccgcgcgat ggtgccgctt ctgctcactc agggcatcgt actgttccag gatacctcac
    12001 tggtgtatgt gttatctctg gccgatttct tccgtaccgc ctcaaccatt ggtgagcgtg
    12061 atggtacgca ggttgagatg atcctgtttg ccggatttgt ttatttcgtt attagtctta
    12121 gcgcgtcgtt gttggtcagc tacttgaaaa gaaggacagc ataatgatta ccctgaaaaa
    12181 tgtttcaaaa tggtatggtc actttcaggt gctgaccgac tgctcaaccg aagtgaaaaa
    12241 aggcgaagtg gtggtggttt gcggcccgtc tggttccggc aaatcaacgc tgattaaaac
    12301 cgtcaacggc ctcgaaccag tgcagcaagg tgaaatcacc gtcgatggta tcgtggttaa
    12361 tgacaagaaa accgatctgg caaagctgcg ttcccgcgtc gggatggtat tccagcattt
    12421 cgagctgttc cctcatctgt cgattatcga aaacctgacc ctggcgcagg tgaaagtgct
    12481 taaacgcgat aaagcgcccg ctcgtgaaaa agcattgaaa ttgctggagc gcgtgggact
    12541 ttccgcacat gccaataaat ttccggcgca gctttccggc ggtcagcagc agcgtgtggc
    12601 aatcgctcgc gcattgtgta tggatcctat tgcgatgctg ttcgacgaac cgacatcggc
    12661 gctggatccg gagatgatca acgaagtgct ggacgtgatg gtggaactgg cgaacgaagg
    12721 aatgaccatg atggtggtga cccacgaaat gggctttgcc cgcaaagtgg cgaatcgggt
    12781 gatctttatg gacgagggta aaattgtcga agactcgccg aaagacgctt tcttcgatga
    12841 tccgaaatcg gaccgcgcga aagacttcct cgcgaaaatc ctgcattaat cactctggcg
    12901 cgcataccca agtgcgcgcc atctcacgct ttatcgtttc gcccctcgta tctgttacat
    12961 cctggcgtta cccttgtcgc aaagaagcac acaacaagga gcaacaatgg cactgccaat
    13021 tctgttagat tgcgacccag gtcatgacga cgctatcgca atagttctcg ccctcgcctc
    13081 accagagctt gatgtcaaag caattacgtc ttccgccgga aaccagacac cagaaaaaac
    13141 cttacgcaat gttctgcgta tgctgacctt gcttaatcgc accgatattc cggtagcagg
    13201 cggcgcggta aaaccgttaa tgcgtgagtt gattatcgcg gacaatgtgc acggcgaaag
    13261 cggtctggat ggcccggcat taccggaacc gacgttcgca ccgcaaaact gtacggcggt
    13321 agagctgatg gcgaaaacgc tgcgcgaaag tgcggaacct gtcacgattg tgtctaccgg
    13381 accgcaaact aacgttgcct tgctgctcaa tagccatccg gaactgcata gcaaaattgc
    13441 ccgtatcgtg attatgggcg gcgcaatggg gcttggtaac tggacgcctg cggctgaatt
    13501 taatatttac gttgacccgg aagcggcaga aattgtcttc cagtcaggga tcccagtggt
    13561 gatggccggt ctggatgtta ctcacaaagc acaaatccac gttgaagaca ccgagcgttt
    13621 ccgcgcgatt ggcaaccctg tttcaaccat tgttgccgaa ctgctggatt tcttcctcga
    13681 atatcataaa gacgaaaaat ggggctttgt cggtgcaccg ctgcatgacc catgcaccat
    13741 cgcctggctg ttgaaacccg agttgtttac cactgttgag cgctgggttg gcgtggaaac
    13801 acaggggaaa tatagccagg gtatgacggt tgttgattat tattatctga ctggcaataa
    13861 accgaatgcc actgtaatgg tcgatgttga tcgtcagggc tttgttgatt tactggctga
    13921 tcgtctgaaa ttttacgctt aacacacgtc attgattaag atcaccacgc gttacctggg
    13981 tggtcttaat ctgtttatct gtttatctct ttttctttca agatataaat gcaatggata
    14041 atgcagaact cgccattggt atcgatctcg gcactaccaa tagtttaatt gccgtctgga
    14101 aagacggtgc cgcgcaatta attccaaata agttcggtga atatttaaca ccatccataa
    14161 ttagcatgga tgaaaataat catattttag tcggaaaacc ggctgtatca cggcgtactt
    14221 cgcatccgga taaaacggca gcgttattta aacgtgcaat gggcagtaat accaactggc
    14281 ggttaggcag cgacacattt aacgcgccag aactgtcctc tttggtatta cgctcattaa
    14341 aagaagatgc cgaagaattt ctgcaacgtc cgattaaaga tgtggtgatc tccgttccgg
    14401 cttatttcag cgatgaacaa cgcaagcata cccgtttagc agcggagtta gccgggttaa
    14461 atgcggtacg cttaattaat gaacccacag cagctgcgat ggcatatggc ctgcatacgc
    14521 aacaaaatac ccgctcgctg gtttttgatc tcggtggcgg cacgtttgac gtcacggtgc
    14581 tcgaatacgc cacgccggtg attgaagttc acgcctccgc tggcgataac ttcctcggtg
    14641 gcgaagattt tacccatatg ttggtcgatg acgttttaaa gcgcgcggat gtcgccagga
    14701 ccacgcttaa cgagagtgaa ctggcagcct tgtacgcctg tgtggaagcg gcaaaatgta
    14761 gcaatcaatc gccattgcac attcgctggc agtaccagga tgaaacgcgg gagtgcgaat
    14821 tttacgagaa cgaactggaa gatttgtggt tgccgctgct caatcgcttg cgagtgccga
    14881 ttgaacaggc gttgcgcgat gcacgtctga agccgagtca aatcgacagt ctggtgctgg
    14941 ttggcggcgc gtcacaaatg ccgctggtgc agcgaatcgc cgtgcgtctg tttggcaaat
    15001 taccgtatca aagttacgac ccgagcacca ttgtcgcgct gggcgcagca atccaggccg
    15061 cctgccgctt acgcagtgaa gatattgaag aggtgatcct cactgatatt tgcccttact
    15121 cgttgggcgt tgaagttaac cgccagggcg tttccggcat tttctcgcca attattgaac
    15181 gaaacaccac agtacccgtg tcgcgtgtag aaacttactc gaccatgcac ccggaacagg
    15241 attctattac ggttaacgtc tatcagggag aaaaccacaa agttaaaaat aacattctgg
    15301 ttgaatcctt cgatgtgccg ttgaagaaaa ccggggctta tcagtcgatt gatattcgct
    15361 ttagttatga tatcaacggc ttgcttgaag ttgacgtgct tctggaagat ggcagcgtta
    15421 agtccagagt cattaatcat agcccggtaa cattgagcgc gcagcagatt gaagagagtc
    15481 ggacgcggtt atccgcattg aaaatttatc cgcgcgatat gctaatcaac cgcaccttta
    15541 aggccaaact ggaagagttg tgggcgcggg cgctgggtga cgagcgagaa gagattggcc
    15601 gggtgatcac cgattttgat gcggcgttgc agtcaaacga tatggcccgc gtcgatgaag
    15661 ttcgtcgacg ggcgagcgat tatttggcta ttgagatccc ttaaacacgg ctgccggatg
    15721 cggcataaac gctgcatccg gcatcggcgc aaaaaacaca tcctcagcta aacaacatcg
    15781 cgatttttcc gagcgcataa cctatcatca gcaccgttaa gctgattttg ataatactgg
    15841 taatgctctg tggtttttca tcctcatcat ttttttcttc atggttttca ggtgttggct
    15901 catcgacatc ttcatcctcc ggctcgagtt tcactttcgt caacagcatc tgggtgataa
    15961 tggcctctaa tggatgagta tctccacctt ccagccttgt attaagacgg gccagcatct
    16021 cttgtttcgg gaacgcttcc agcacaacct gttccgctac ccgatacaaa tccagtaacg
    16081 gtgaatcttg ctctgtctcg ctaccgactc tcgatttcca ccacgcccat gctttgcgct
    16141 tgaagaataa cgactcaata aacgattgac ctaacggcga taattttccg ctatgtaaaa
    16201 tctccgccca taacagccgc gtccgggcgc tctcaccagg aacatatttc aacggtttac
    16261 cttgcgcatc taccatgctt cgcgcttcga taaccatcac taataacgcg aaataatccg
    16321 ggcaatgacg cttgctccac tgcaacaaca aattatcagc ttgtgtagat gggtaacttt
    16381 cccagtacgc gcagagatcg gcaagcaggc tttccccctc gccgcagtac aaacgttgtg
    16441 cacactggta ataccgggca tcttcgcttt ctggttcttc cgcttgccac tgtttggcta
    16501 caggaatgag ttccgccata cctgtttgca ccgagctgta ccagcgcaac agtttgcgat
    16561 gtaactttgc atcatcagga atgaaccacg gtccttccat cgccaaaaac gcagtgacat
    16621 agtcaggatg gtaacggcag caacgttcca gcgcgaagta atattcaaca gtctggttct
    16681 gtaacgcgac gggatattcc agaaggctaa ggaaatcgaa acaatcacca gcctgaatgg
    16741 cctcaagaaa agcctccctt tcttcctcat catccattcc ttcgctgtca ttaaacgaat
    16801 gccagttcaa acgctctgcc aacaaagaag cacaatttaa tgaaagatat cgcgcttcta
    16861 tagcgattgc acacagcggc cagcgtaatt gatcgacatc ctccatgttc caggtattta
    16921 attgctgaat aaatttctgc caggcggagg gctgaaaacg atcactttct gaatccagta
    16981 atgtcctgaa tgcacgtagt atttcatgtt cagcggtggc atctttttct tcatcatcgg
    17041 cttcctcgac aggatttacc gccagtcgca gcgcctcttc ataggcctgg cgtaatgctt
    17101 taaacccctg cggatcggtt tcgggatgac acaacggtaa gcgagcaaga taagcctggc
    17161 ggataatgtc tatttgcgtc gtgctttcaa tttcgaggat ttgccaacat gttttcatag
    17221 gtcgctcaca tccttaagcg ttaccgggca ttcaggctgg acagttaatg gttgccaggg
    17281 aagcgtggca tagaaacggt cgcttcccgc tgccagctct tcgaacatcc gactcccggc
    17341 gctatcgtat ttgtacaggg ttagcgcctc gcgtagcgaa gcgttgtctt cgcactgaga
    17401 agattgccag tagagacgcc ctagtgaata ggcagaaaaa tagtgcatcc agctgtgata
    17461 gtaatggtga gctcgtgcat agacgcgcga ttgcagccat aaactctctt cttccgtcag
    17521 ccagttgttg agcacaccga cccgtgacag aaaccccatt cgaacataat cccaggcttt
    17581 gattccccct tctccacagc acatagcggt ttgggcaacg aattgggtat aaatcaaccc
    17641 tctctccgag ccaccagcaa ttaatgcttt cctctcttca ggggaagagc gttgccatgt
    17701 cagatataag ccagctaaat cattggcatg accatcatca gtcatctgat aaatagtctg
    17761 aattaaatca tcacgattat ttattcccca ggatgatgag agatcgaatt cttttatccc
    17821 agtatacatt tttggcgagt catgcgcggg tttgcaactc tgattaagta tttccatggg
    17881 cgcagaaagc gcaaaaagaa gatattgttc ttccttattc attttttaat aatcccttaa
    17941 gtgcattaac tgactacttt tttattattg atgagtataa acttgacgct ttcaaaataa
    18001 aaagaaaatc gaagcattca cacatgaata aaaaatcaat gtatatattc gcaattttta
    18061 tagttgcagc aattacctgt attagccaac ccaagaaaac gacgttgcgt gataaagcca
    18121 tggtgaatta tgcctttgat tatttaagat caccaggcag tctaccgtta accacggcag
    18181 ccacggagct ttccgcgatt catggtcact caacatcgca atatcgcctt ggcgaatttt
    18241 atcttcatgg tagcgacggt aaaccactgg attatacaca ggcgagatac tggtatgagc
    18301 aatcagcaga acaggaaaat ccgcgcgcac aaagcaaact ggggtggatc tacctgaaag
    18361 gcctgggagt caaacccgat acccgtaaag caattctctg gtataaggaa gcagctgaac
    18421 aagggtatgc tcatgctcaa tatactttgg gtttgatcta cagaaatggc actggtatta
    18481 atgttaacca ttatgaatct caaaaatggt taaaactggc cgccaaacaa cattacaaaa
    18541 atgcggaaag attacttgcc ggacttcccg cacattaacg tcgactgtca cccgaagaga
    18601 tggagtattt ttcctatcaa accggcaaag ataaaaatgt agaaaataat tttgatgacg
    18661 cccagacagc ccagcccgcc atcgtcaacg ggcattggct gcggttgcgg ctccggctcg
    18721 acggtttcct gaccggtaaa attcgccgcc tgaattaata atcgacgaat aattaatgct
    18781 tccagtggac aacgatcttc tgcatcaagg cggctcacaa tcggcattaa tgattcttct
    18841 gtcggttggc tggcctgcac taattgtttg gcaactaaat ataatggcca taagggatgt
    18901 tctgtatcac gcgtgagtga gatccgcggc ttacgtcctt tgtgcaaaac catctcgacc
    18961 agtgaacgcc ccagcgcaga gagtttttca tcgctgagta tttcgtataa atgaaaacgc
    19021 gtacgggcgc tgtcgccggg gacataaagt agctgtttac cctgatcgtt aaccagttca
    19081 cgagcttcaa tcatcatcac cactaacggg taataatcga cccgatgctg acggcaccat
    19141 tgcaatatta aagcatccgc ctgggtggag ggatactcgc gccagtagtc acaaagttct
    19201 gccagcaagc tttcgccttc tccacaatag acgcgttgcg cgtattcata ataacgcgca
    19261 tcttcattat caggttcagc ttcccgccac gaccgggcgt aatccagaag ttcggggata
    19321 tccaggcgac ctgcgctata ccagcggagt aaatttctgt gcagttttgc atcatcgggg
    19381 atgatcactg cgcgatgttg cgctaaatac aatgtcagcc attctggatg cgatgaccaa
    19441 atccgagcga acatttggta aaaatcgatg gtgtcatttt gcacggcaac gggcagatgc
    19501 agaatggtct ggaagttaaa aacattcccc ttcgcaatgg catataaaaa ggattccagt
    19561 tcttcttcgt ctatttcccc gacgttttcc tcctgcaacc accgcaatct ttctgctaat
    19621 aacaccacgc actcgaagga taaatgggca gtgttcatgg cgattgtgca cagcgaccag
    19681 cgtaattcat caatctcttc catcgagcaa taatttaatt gctgaatgaa tcgctgccag
    19741 gcggagggca gaaaacgttc gctatcagag gcaagtaacg cgcgaaaggc gagcagaatt
    19801 tcatgttctg ctacctcata ttcttctggt tgccaaacag atttagcagg cgactgcgca
    19861 atccggagcg cctcctcata cgcctgacga agttgtttaa aaccctgcgg atcggtttct
    19921 ggatgaaagg acagtaacag cgccagataa gcgcggcgga taatatcgac gtcagtcgtt
    19981 tcctctattt cgaggatctt ccagcaattt ttcataggtc gctcatatcc ttaagcgttt
    20041 ccgggcatgt ggggtagtca gccagtggtt gccaggacaa ggcagggtaa tacgcatcat
    20101 cactggcaaa taattgataa aacatatcat tgccggaatc gtcgtattct ttacgagcta
    20161 agaattcgcg taataaaggc agatgatcat cttcgggaga ctgccaatat tgccgaccaa
    20221 aggtataacc ggcaaagtat tgccgccagt tactataata acgtagagcc cgtagatgaa
    20281 tacgcgactg gatccataga ctttcttctt cactcaacca gttattaagt acgcccattc
    20341 gacttaaaaa acccatccgg acataatccc atgctttaat tcctccctca ccacagcatt
    20401 cggctgtgct ggcgacaaat tgcgcataag cctgaccttg ctcatttagt tcagcaagat
    20461 agtcacgcca ttcacatggc gaataacgaa accagcggtg ataaaaccct gccaaccgag
    20521 cggcatgacc gttatcgatc attcggtgaa ttgtgcgcag caggtcatca cggtcatcaa
    20581 ttccccatga ggaagaaagg tcaaaaaagc atgtacctaa atacattttg ggggatgaat
    20641 gcgaaggtaa acagcattca ttgcgtattt ccatcggagt ggaaagtgca aacaatattt
    20701 tttgcgaccc catgtccata taatttatcc ttaaacattc atttaatgaa tgtaaactac
    20761 atttcgagac tcagttattt atacttttct gacgcaacat aatacgcata aaataaggct
    20821 tacgcaaaaa tggaacacat aaaaatataa tatgttttat ctacaaccat aaggaaatga
    20881 taatggtttt tacgtcaagt tgctgcgata atttatcaat agatgagatt atcgaacgtg
    20941 ctgaaaaagg cgattgcgag gctcaatata ttgttgggtt ttattataat cgcgatagcg
    21001 caattgactc tccggacgac gaaaaggcct tttactggct gaagctggcc gctgagcaag
    21061 gtcattgtga agcacagtat tccttagggc aaaagtatac cgaggataaa agccgtcata
    21121 aagataatga gcaagccatc ttctggctga aaaaagctgc cctacaaggc catactttcg
    21181 cttccaacgc ccttggctgg acactggatc gtggagaagc ccccaattat aaagaagcgg
    21241 ttgtctggta tcagatagcc gcggagagcg gaatgtctta tgcgcaaaat aatcttgggt
    21301 ggatgtacag aaatggcaac ggagtcgcaa aagactatgc gctggcattt ttctggtaca
    21361 aacaagctgc attacaaggc catagtgacg cgcaaaacaa tctggccgat ctttatgaag
    21421 acggaaaagg cgttgctcaa aacaagacac tcgccgcatt ctggtatttg aaaagcgcac
    21481 agcagggtaa tcggcacgcc cagtttcaaa ttgcatggga ttataacgct ggcgaagggg
    21541 tggaccagga ctataagcaa gcgatgtact ggtatctgaa ggctgccgct caggaaagcg
    21601 tcggcgctta cgtcaacatc ggttatatgt ataaacacgg acaaggcgtt gagaaagatt
    21661 atcaggctgc ctttgaatgg tttacgaaag ccgctgaatg caatgacgcc actgcctggt
    21721 ataacctggc cattattatc actacggaga aggaagacct gtcgatctcc gacaggctct
    21781 cgacctgtat cgtaaagttc agtcatccgg aaccagagat gtcagtcagg aaattcgtga
    21841 gactgaagat ttactgtaga gataaaaaca agggccgggg aatttcattc ggcccatatt
    21901 ctctttattg ctgtgcgaaa tcagactatt acggctcaaa cgggcgtctc tggaactggt
    21961 catgaccaca tttcgggcat agcgtcagca cttccggtgt gtagatcggg agatggaagt
    22021 gacatttctc gcagaccaga tttcccagcc cgaccacttc tccgctgtga taaaccccat
    22081 gatgattgag gtcctggaaa acttcgcgcc attcaagctg cgttttatcg gtgatatctg
    22141 ccagctcctg ccataagctt tctttaatca cccgcataaa gacgctgtca gattcttctt
    22201 tcaggctctc ttcatagctc atggcgaact cttccaggtc acgtcttaca gctcgcgtca
    22261 gctcatcgac ctcggttcgc gttaactccc ctgtttttat tacgcgctcg cgcgcctgtt
    22321 ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg ttcgctcagt gacgcgacca
    22381 gttcacggta atattgagca accttgttca tcgtttcatc tcccgggtcg ttaactgact
    22441 ataataatag acgttctttg ttccttgctt tgctaatacg cccacaaact ctgtaaatta
    22501 atgtatgaat aatccgggca tcatttcaac gtcgagtgcg cgaaaggctg ttttgacgcg
    22561 gacgtttggg ctatgctatg cggatctgaa aaaccacctc aacgctacat ttgtagccgt
    22621 attgaaaaca ggaccactgg ctgccatgca agagcaatac cgcccggaag agatagaatc
    22681 caaagtacag cttcattggg atgagaagcg cacatttgaa gtaaccgaag acgagagcaa
    22741 agagaagtat tactgcctgt ctatgcttcc ctatccttct ggtcgactac acatgggcca
    22801 cgtacgtaac tacaccatcg gtgacgtgat cgcccgctac cagcgtatgc tgggcaaaaa
    22861 cgtcctgcag ccgatcggct gggacgcgtt tggtctgcct gcggaaggcg cggcggtgaa
    22921 aaacaacacc gctccggcac cgtggacgta cgacaacatc gcgtatatga aaaaccagct
    22981 caaaatgctg ggctttggtt atgactggag ccgcgagctg gcaacctgta cgccggaata
    23041 ctaccgttgg gaacagaaat tcttcaccga gctgtataaa aaaggcctgg tatataagaa
    23101 gacttctgcg gtcaactggt gcccgaacga ccagaccgta ctggcgaacg aacaagttat
    23161 cgacggctgc tgctggcgct gcgataccaa agttgaacgt aaagagatcc cgcagtggtt
    23221 tatcaaaatc actgcttacg ctgacgagct gctcaacgat ctggataaac tggatcactg
    23281 gccagacacc gttaaaacca tgcagcgtaa ctggatcggt cgttccgaag gcgtggagat
    23341 caccttcaac gttaacgact atgacaacat gctgaccgtt tacactaccc gcccggacac
    23401 ctttatgggt tgtacctacc tggcggtagc tgcgggtcat ccgctggcgc agaaagcggc
    23461 ggaaaataat cctgaactgg cggcctttat tgacgaatgc cgtaacacca aagttgccga
    23521 agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact ggctttaaag ctgttcaccc
    23581 attaacgggc gaagaaattc ccgtttgggc agcaaacttc gtattgatgg agtacggcac
    23641 gggcgcagtt atggcggttc cgggtcacga ccagcgcgac tacgagtttg cctctaaata
    23701 cggcctgaac atcaaaccag ttatcctggc tgctgacggc tctgagccag atctttctca
    23761 gcaagccctg actgaaaaag gcgtgctgtt caactctggc gaattcaacg gtcttgacca
    23821 tgaagcggcc ttcaacgcca tcgccgataa actgactgcg atgggcgttg gcgagcgtaa
    23881 agtgaactac cgcctgcgcg actggggtgt ttctcgtcag cgttactggg gcgcgccgat
    23941 tccgatggtg acgctggaag acggtaccgt aatgccgacc ccggacgacc agctgccggt
    24001 gatcctgccg gaagatgtgg tgatggacgg cattaccagc ccgattaaag cagatccgga
    24061 gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt gaaaccgaca ctttcgacac
    24121 ctttatggag tcctcctggt actatgcgcg ctacacttgc ccagagtaca aagaaggtat
    24181 gctggattcc gaagcggcta actactggct gccggtggat atctacattg gtggtatcga
    24241 acacgccatt atgcacctgc tctacttccg cttcttccac aaactgatgc gtgatgcagg
    24301 catggtgaac tctgacgaac cagcgaaaca gttgctgtgt cagggtatgg tgctggcaga
    24361 tgccttctac tatgttggcg aaaacggcga acgtaactgg gtttccccgg ttgatgctat
    24421 cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa gatgcggcag gccatgaact
    24481 ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac aacggtatcg acccgcaggt
    24541 gatggttgaa cgttacggcg cggacaccgt tcgtctgttt atgatgtttg cttctccggc
    24601 tgatatgact ctcgaatggc aggaatccgg tgtggaaggg gctaaccgct tcctgaaacg
    24661 tgtctggaaa ctggtttacg agcacacagc aaaaggtgat gttgcggcac tgaacgttga
    24721 tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg cataaaacga tcgctaaagt
    24781 gaccgatgat atcggccgtc gtcagacctt caacaccgca attgcggcga ttatggagct
    24841 gatgaacaaa ctggcgaaag caccaaccga tggcgagcag gatcgcgctc tgatgcagga
    24901 agcgctgctg gccgttgtcc gtatgcttaa cccgttcacc ccgcacatct gcttcacgct
    24961 gtggcaggaa ctgaaaggcg aaggcgatat cgacaacgcg ccgtggccgg ttgctgacga
    25021 aaaagcgatg gtggaagact ccacgctggt cgtggtgcag gttaacggta aagtccgtgc
    25081 caaaatcacc gttctggtgg acgcaacgga agaacaggtt cgcgaacgtg ctggccagga
    25141 acatctggta gcaaaatatc ttgatggcgt taccgtgcgt aaagtgattt acgtaccagg
    25201 taaactcctc aatctggtcg ttggctaagc gcgggaggaa gcgtgcgata tctggcaaca
    25261 ttgttgttat ctctggcggt gttaatcacc gccgggtgtg gctggcatct gcgtgatacc
    25321 acgcaggttc cttccactat gaaggtcatg atcctggact caggcgatcc gaacgggcca
    25381 ttaagccgtg cagtgcgtaa ccagttacgt ctgaatggtg tcgagttgct tgataaagaa
    25441 accacgcgta aggacgttcc atccctgcgt ttgggtaaag tgagcatcgc gaaagatacc
    25501 gcatcggtat tccgtaacgg tcaaacagca gagtatcaga tgatcatgac ggtgaatgcg
    25561 accgtgttga tccccggccg tgatatctac ccgattagcg ccaaagtctt ccgttcgttc
    25621 tttgataacc cgcaaatggc gttagcgaaa gataacgaac aagacatgat cgtaaaagag
    25681 atgtacgacc gtgctgccga acagctgatt cgtaagctgc caagcatccg tgctgcggat
    25741 attcgttccg acgaagaaca gacgtcgacc acaacggata ctccggcaac gcctgcacgc
    25801 gtctccacca cgctgggtaa ctgatgattc ggttgtaccc ggaacaactc cgcgcgcagc
    25861 tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa cgatcctctg ttattgcagg
    25921 aaagccagga cgctgttcgt caggtagctg cggcacaagg attcgaagaa caccacactt
    25981 tttccattga tcccaacact gactggaatg cgatcttttc gttatgccag gctatgagtc
    26041 tgtttgccag tcgacaaacg ctattgctgt tgttaccaga aaacggaccg aatgcggcga
    26101 tcaatgaaca acttctcaca ctcaccggac ttctgcatga cgacctgctg ttgatcgtcc
    26161 gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg gtttactgct cttgcgaatc
    26221 gcagcgtgca ggtgacctgt cagacaccgg agcaggctca gcttccccgc tgggttactg
    26281 cgcgcgcaaa acagctcaac ttagaactgg atgacgcggc aaatcaggtg ctctgctact
    26341 gttatgaagg taacctgctg gcgctggctc aggcactgga gcgtttatcg ctgctctggc
    26401 cagacggcaa attgacatta ccgcgcgttg aacaggcggt gaatgatgcc gcgcatttca
    26461 ccccttttca ttgggttgat gctttgttga tgggaaaaag taagcgcgca ttgcatattc
    26521 ttcagcaact gcgtctggaa ggcagcgaac cggttatttt gttgcgcaca ttacaacgtg
    26581 aactgttgtt actggttaac ctgaaacgcc agtctgccca tacgccactg cgtgcgttgt
    26641 ttgataagca tcgggtatgg cagaaccgcc ggggcatgat gggcgaggcg ttaaatcgct
    26701 taagtcagac gcagttacgt caggccgtgc aactcctgac acgaacggaa ctcaccctca
    26761 aacaagatta cggtcagtca gtgtgggcag agctgaaagg gttatctctt ctgttgtgcc
    26821 ataaacccct ggcggacgta tttatcgacg gttgatatga aatctttaca ggctctgttt
    26881 ggcggcacct ttgatccggt gcactatggt catctaaaac ccgtggaaac gctggcgaat
    26941 ttgattggtc tgacgcgggt cacaatcatc cctaataatg ttcctccgca tcgtccccag
    27001 ccggaagcga acagcgtgca gcgtaaacac atgcttgaac tggcgattgc cgacaagcca
    27061 ttatttactc ttgatgaacg cgagctaaag cgcaatgccc cctcttacac tgcgcaaaca
    27121 ctgaaagagt ggcggcagga acaaggaccg gacgtgccgc tggcgtttat tattggtcag
    27181 gattcactgc tgacctttcc gacctggtac gaatacgaaa cgatactcga caatgcacat
    27241 ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg cgcaaccgca ataccagcaa
    27301 tggctggaag atcatttgac acataacccg gaagatcttc accttcagcc tgccggtaaa
    27361 atttatctgg ctgaaacgcc gtggtttaac atctcggcga ccatcatccg cgaacgtttg
    27421 caaaacggtg aatcgtgtga ggatttattg ccggaaccgg tactgactta cattaaccaa
    27481 caaggcttgt atcgctgata cctgtcgtta cggagggcat gatgcgactg tggttaattc
    27541 gtcatggtga aacgcaagcg aatatcgatg gtctttacag cggtcatgcg cccacccccc
    27601 tgaccgcgcg cggtattgag caagcgcaaa atctgcatac gctgctacat ggtgtttcct
    27661 ttgatctggt tttatgcagt gaactggaac gggcacagca taccgcacga ctggttctca
    27721 gtgaccgcca gctccccgtg caaatcatcc ctgaactcaa cgaaatgttt tttggcgact
    27781 gggagatgcg acatcatcgc gacctcatgc aagaagatgc cgaaaactat agcgcgtggt
    27841 gcaatgactg gcagcatgca atccccacga acggtgaagg atttcaggca ttttcgcaac
    27901 gtgtggaacg ctttatcgca aggcttagtg aatttcagca ctatcagaat attttagtcg
    27961 tcagccatca gggtgtactg agtctgttaa tcgcccgttt aattggcatg cctgccgagg
    28021 ctatgtggca ttttcgcgtt gactaggggt gttggagcgc cattgatatc aaccaaaaat
    28081 tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt cgagaatgca tgacttttct
    28141 gtttttttac gggtaagccg caacgaccat tgacagaccc gggcaggctg atattctccg
    28201 cagccagact tttttcgcca gacacgactt tgtagaaatt gttttacaaa aatggcgatg
    28261 caatctgcgg cgcggggtgg gatgatagcc cactttcgaa agccgattcg gcgacaattg
    28321 tcccgaaatc gcctctggtt caggtatact gacagaccat ttttatctat ttgattcacc
    28381 cagggggaaa acttgcaggg taaagcactc caggattttg ttatcgacaa aattgatgac
    28441 ctcaaaggtc aggacatcat cgccttagac gttcaaggca aatccagcat caccgactgc
    28501 atgatcatct gtacgggtac gtccagccgt catgttatgt ccattgctga ccacgttgtg
    28561 caggagtctc gcgcagcggg cctgttaccg ctcggcgtag aaggtgaaaa cagcgccgac
    28621 tggattgtcg tggatttggg cgatgtgatt gtccatgtca tgcaggaaga gagccgtcgc
    28681 ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc tgcaacttgt cgccgtggga
    28741 acgaaaatgc cggactgggt acaaaccggt tttaccgagt acctgcgtcg ttttccgaaa
    28801 gatatgccct tcgagctgat tgaaattccg gccggaaaac gcggcaagaa tgcggacatc
    28861 aagcgcatac tcgacaaaga gggtgagcag atgttggcgg ccgcaggcaa aaaccgcatt
    28921 gtcaccctcg atattccagg caagccctgg gatacgccgc agttagccgc tgagctggaa
    28981 cgctggaagc tggatggtcg cgacgtcagt ctactgattg gcgggcctga agggttgtcg
    29041 cctgcctgta aagcggcggc ggagcagagc tggtcgctgt cggcgcttac cctcccccat
    29101 ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg cgtggagcat caccaccaac
    29161 catccttatc accgtgagtg ataagggagc tttgagtaga aaacgcagcg gatgaaacta
    29221 cagaactctt ttcgcgacta tacggcagag tccgcgctgt ttgtgcgccg ggcgctggtc
    29281 gcctttttgg ggattttgct gctgaccggc gtgcttatcg ccaacctgta taatctgcaa
    29341 attgttcgct ttaccgacta ccagactcgc tctaatgaaa accgcattaa gctggtgcct
    29401 atcgcgccca gccgcggcat tatctacgac cgtaatggta tccctctggc cctcaaccgc
    29461 actatctacc agatagaaat gatgccagag aaagtcgata acgtgcagca aacgctggac
    29521 gctttgcgca gcgtggttga tctgaccgat gacgatattg ctgcattccg aaaagagcgc
    29581 gcacgttcac accgtttcac ctctattccg gtgaaaacta acctgaccga agtacaagta
    29641 gctcgctttg ccgtcaatca gtaccgtttt ccgggtgtcg aagttaaagg ctataaacgt
    29701 cgttactatc cttacggttc ggcgttgacc cacgtcatcg gctatgtgtc gaaaatcaac
    29761 gataaagacg tcgaacgcct gaataatgac ggcaaactgg ccaactatgc ggcaacgcat
    29821 gatatcggta agctgggcat tgagcgttac tatgaagacg tgctgcacgg tcagaccggt
    29881 tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc gccagttaaa agaagtacca
    29941 ccgcaagccg gacacgatat ttacctgacg ctggatctca aactccagca atatattgaa
    30001 acgctgctgg cgggtagccg cgcagctgtg gttgtcaccg atccgcgtac aggtggggtg
    30061 ctggcgctgg tttccacgcc tagttatgac ccaaacctgt ttgttgacgg tatctccagc
    30121 aaagattatt ccgccttgtt gaacgccccg aatacaccgc tggtgaaccg cgccacacag
    30181 ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg cggtttcggc attgagcgcc
    30241 ggggtgatca cgcgcaatac gacgctgttt gacccaggct ggtggcaact gccaggttcg
    30301 gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc gtctgaatgt cacaagatcg
    30361 ctggaagaat ctgcggatac cttcttctat caggtggcct acgatatggg gatcgatcgc
    30421 ctctccgaat ggatgggtaa attcggttat ggtcattaca ccggtatcga cctggcggaa
    30481 gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga aacgctttaa aaaaccgtgg
    30541 tatcagggtg acaccattcc ggttggtatc ggtcagggtt actggacagc gaccccaatc
    30601 cagatgagta aggcactgat gatcctgatt aatgacggta tcgtgaaggt tcctcatttg
    30661 ctgatgagca ccgccgaaga cggcaaacag gtgccatggg tacagccgca tgaaccgccc
    30721 gtcggcgata ttcattccgg ttactgggag ctggcgaaag acggtatgta cggtgttgct
    30781 aaccgcccta acggtacggc gcataaatac tttgccagcg caccgtacaa aattgcggcg
    30841 aaatccggta ccgctcaggt cttcggtctg aaagcgaacg aaacctataa tgcgcacaaa
    30901 attgccgagc gtttacgtga ccacaaactg atgaccgcct ttgcgccata caacaatccg
    30961 caagtggctg tcgccatgat tctggagaac ggtggtgcgg gtccggcggt tggtacactg
    31021 atgcgccaga tcctcgacca cattatgctg ggtgataaca acaccgatct gcctgcggaa
    31081 aatccagcgg ttgccgcagc ggaggaccat taatcatgac ggataatccg aataaaaaaa
    31141 cattctggga taaagtccat ctcgatccca caatgctgct gatcttactg gcattgctgg
    31201 tttacagcgc cctggttatc tggagcgcca gcggtcagga tattggcatg atggagcgta
    31261 aaatcggcca aatcgcgatg ggtctagtca tcatggtggt gatggcgcaa attcctccac
    31321 gcgtttatga aggctgggcc ccctatctct atatcatctg tattattttg ctggtggcgg
    31381 tagatgcttt cggtgccatc tctaaaggtg ctcaacgctg gctggacctc ggtattgttc
    31441 gttttcagcc gtcggaaatt gccaaaatag ccgtaccact gatggttgcg cgctttatca
    31501 accgcgacgt ttgcccgcca tcgttgaaga acactggcat cgcgctggtg ctgatattta
    31561 tgcccacgct gctggtggct gcacagcctg acctgggaac atcaatcctc gttgcgcttt
    31621 ccggtctgtt tgtactgttc ctctctggcc ttagctggcg tctgattggc gtcgcagtag
    31681 tgctggtggc ggcgttcatt ccgattctgt ggttcttcct gatgcatgat taccagcgcc
    31741 agcgcgtaat gatgctccta gacccggaat cagacccact cggcgcgggc tatcacatta
    31801 ttcagtctaa aattgctatt ggctccggcg gattacgcgg caaaggctgg ctgcacggca
    31861 ctcagtcaca gcttgaattt ctccccgaac gccataccga ctttatcttc gcggtactgg
    31921 cggaagagct gggattagtg ggcattctga ttctgctcgc tctctacatt ctgctgatca
    31981 tgcgcgggct gtggatagcc gccagagcgc aaaccacctt tggtcgcgtc atggctggtg
    32041 gcttaatgct gatattattc gtttatgtct tcgtaaatat tggtatggta agcggtattc
    32101 tgccggttgt aggggttccg ctcccactgg tcagttatgg aggatcggcg ctaattgtgc
    32161 tgatggctgg gttcgggatt gtaatgtcaa tccacaccca caggaaaatg ttgtcgaaaa
    32221 gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga tctgcatcgc ggcaggaatg
    32281 ctcgcggcat gtacaagcga tgatggtcag caacagacgg taagtgtacc gcagcctgcg
    32341 gtatgtaacg gccccatagt tgaaattagc ggggcggacc cgcgtttcga accactgaac
    32401 gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct acaaaatcgt gcaggatccg
    32461 tctcgattta gccaggcggg actggcggca atctatgatg ccgaacccgg cagtaacctg
    32521 acggcctctg gcgaagcttt cgatccgaca cagctgacgg cggcccatcc aacgcttccg
    32581 atccccagct acgccagaat cactaacctg gctaacgggc gaatgatcgt ggtgcgcatt
    32641 aatgatcgcg gcccttacgg caacgaccgc gttatttcac tttctcgcgc ggcagctgac
    32701 cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc cgattattgt cgcccaggat
    32761 ggttcgcttt ctggtcctgg tatggcttgt accacagtcg ccaaacagac ttacgccctg
    32821 ccagcacctc ccgatttaag cggtggcgcg ggaacaagtt cagtgtctgg cccgcagggt
    32881 gacattcttc cggtcagtaa ttcgacgcta aaaagcgaag atccgaccgg cgcgccggta
    32941 accagtagcg gtttcctcgg cgctccaacg accttagcgc ctggtgtact ggaaggcagc
    33001 gaaccgacgc ctgcgccaca gcccgttgtt acagctccgt cgacaacgcc tgcaacctcg
    33061 cctgcaatgg tgacaccgca agccgcctcg caaagcgcca gcggcaactt tatggtgcaa
    33121 gtcggggccg taagcgatca ggctcgtgcg caacagtacc aacagcaact gggacagaag
    33181 ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct ggcggatcca gcttggccca
    33241 ttcgccagca aagccgaagc cagtaccttg cagcaacgtt tgcaaaccga agcccaatta
    33301 cagtcattta ttactaccgc gcagtagcgt aaagcaggca tctgatgtgt caatttctgt
    33361 aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg ccggtagtgc atttgctata
    33421 gtagggcact tttttaattc catcacggat gtcgtagttc agaccatgaa taccattttt
    33481 tccgctcgta tcatgaagcg cctggcgctc accacggctc tttgcacagc ctttatctct
    33541 gctgcacatg ccgatgacct gaatatcaaa actatgatcc cgggtgtacc gcagatcgat
    33601 gcggagtcct acatcctgat tgactataac tccggcaaag tgctcgccga acagaacgca
    33661 gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga ccagttacgt tatcggccag
    33721 gcaatgaaag ccggtaaatt taaagaaact gatttagtca ctatcggcaa cgacgcatgg
    33781 gccaccggta acccggtgtt taaaggttct tcgctgatgt tcctcaaacc gggcatgcag
    33841 gttccggttt ctcagctgat ccgcggtatt aacctgcaat cgggtaacga tgcttgtgtc
    33901 gccatggccg attttgccgc tggtagccag gacgcttttg ttggcttgat gaacagctac
    33961 gttaacgcac tgggcctgaa aaatacccac ttccagacgg tacatggtct ggatgctgat
    34021 ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc aggcattgat ccgtgacgta
    34081 ccgaatgaat actcgatcta taaagaaaaa gaatttacgt ttaacggtat tcgccagctg
    34141 aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg acggcatcaa aaccggacac
    34201 actgacaaag caggttacaa ccttgttgct tctgcgactg aaggccagat gcgcttgatt
    34261 tctgcggtga tgggcggacg tacttttaaa ggccgtgaag ccgaaagtaa aaaactgcta
    34321 acctggggct tccgtttctt cgaaaccgtt aacccactga aagtaggtaa agagttcgcc
    34381 tctgaaccgg tttggtttgg tgattctgat cgcgcttcgt taggggttga taaagacgtg
    34441 tacctgacca ttccacgtgg tcgcatgaaa gatctgaaag ccagctatgt gctgaacagc
    34501 agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg gaactatcaa cttccagctt
    34561 gatggcaaaa cgatcgagca acgcccgctg gttgtgttgc aagaaatccc ggaaggtaac
    34621 ttcttcggca aaatcattga ttacattaaa ttaatgttcc atcactggtt tggttaaaaa
    34681 ttgaacactt gaaagtgtaa tttccgtccc catatactaa gcatcagtaa aaaactcccg
    34741 ccttctggcg ggagttgcta tttaattacg ttacgccgga gctgacatga aaaccaaact
    34801 taacgaactg cttgaattcc ctactccttt tacttacaaa gttatggggc aggcgttacc
    34861 tgagctggtt gatcaggtgg ttgaagtggt acagcgccat gcgccaggtg actacacccc
    34921 aacggtaaaa ccaagcagca aaggcaacta ccactcggta tctatcacta tcaacgccac
    34981 tcatatcgag caggttgaaa cactgtatga agaactaggc aaaatcgata ttgtccgcat
    35041 ggttctgtaa ctcgcttctc cgttacccgg cccctggtcg ggtaactccc ctctcctcgc
    35101 tgtgatatac ttttccccca cttttactca ttctccacgg agatgcagtt ttgtatcagg
    35161 ataaaattct tgtccgccag ctcggtcttc agccttacga gccaatctcc caggctatgc
    35221 atgaattcac cgatacccgc gatgatagta cccttgatga aatctggctg gtcgagcact
    35281 atccggtatt cacccaaggt caggcaggaa aagcggagca cattttaatg ccgggtgata
    35341 ttccggtgat ccagagcgat cgcggtgggc aggtgactta tcacgggccg gggcaacagg
    35401 tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa ctggtgacct
    35461 tgcttgagca aacagtggtg aataccctgg ctgaactggg tatagaagcg catcctcggg
    35521 ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg ctcactgggt ttacgtattc
    35581 gacgcggttg ttcattccac ggtctggcat taaacgtcaa tatggatctt tcaccatttt
    35641 tacgtattaa tccttgtggg tatgccggaa tggaaatggc taaaatatca caatggaaac
    35701 ccgaagcgac gactaataat attgcaccac gtttactgga aaatatttta gcgctactaa
    35761 acaatcctga cttcgaatat attaccgctt aattgcatac ctcaatggcc caatttacac
    35821 tgggtcatta ctcataatac ctgctaatgc tttacatctc cttcgcgttt gtatactttc
    35881 tttatcactt gcagaaatgc ggtgctacgc cttgctccgt aactcacccc attgattaac
    35941 ctattgaaat agttgattat ggtgagcaaa agcgttccgt tccaattaga cataagatta
    36001 ttgtaatttt tttaaatatt atttttccat gaataaaatt ggagtaagtg cgtggatagt
    36061 aataatcaaa ttgaaccctg tttaagcaga aagtcgtcag aaggtaaacc acaaatattt
    36121 acaaccttac gaaatattga tcttaacctt ctgactattt ttgaagctgt atatgtacat
    36181 aaagggatcg ttaatgcagc gaaagtgctt aatctgaccc cctcggcaat cagtcagtct
    36241 attcagaaac tgcgcgttat attccctgac ccattgttta ttcgcaaagg ccagggtgtc
    36301 actcctaccg catttgcgat gcatctacat gagtatatca gtcagggcct tgagtccatt
    36361 cttggcgcgc tggatatcga aggaagctat gataagcaac gaacgataac tattgcgacc
    36421 actccctcgg tcggagccct ggtccttcct gtcatctatc gggcgattaa aactcactat
    36481 ccgcagcttt tactgcgcaa cccgcccatc agcgacgcgg aaaaccaact cagtcagttt
    36541 caaaccgatc tcatcatcga taacatgttt tgcaccaatc gtacggtgca acatcatgtt
    36601 ctgttcaccg acaatatggt gttaatttgc cgcgagggaa atccactact ctctttagaa
    36661 gatgacagag agactatcga caacgctgcg catgtactcc tgttaccgga agggcaaaat
    36721 ttcagcggtc tgcggcagag agttcaagag atgtttccgg accggcaaat caatttcacc
    36781 agctacaaca ttttgacaat cgctgcactg gttgccaaca gtgacatgtt agcgattatt
    36841 cccagccgtt tttataacct gtttagtcgt tgctggccgc tggaaaaact gccttttccg
    36901 tccttaaatg aggagcaaat agatttctct atccactaca acaaattcag cctgcgtgat
    36961 cccatcctgc acggggtaat agatgttatc cgtaatgcat tttgaaaaag tattgttaca
    37021 gccagatcac agcgcagaac gataaaatgg cacaatccaa caacaatttt acattttcgc
    37081 gaccgctttg gctgctttca ggtccgtttc aatgatatac tgccagtcgt taattcaaaa
    37141 atagttgata attacaacaa tctattgaat tgaaacgctt tccttcgtaa ttcgcaactg
    37201 gaacacgcac gctatgagta aacccattgt gatggaacgc ggtgttaaat accgcgatgc
    37261 cgataagatg gcccttatcc cggttaaaaa cgtggcaaca gagcgcgaag ccctgctgcg
    37321 caagccggaa tggatgaaaa tcaagcttcc agcggactct acacgtatcc agggcatcaa
    37381 agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag gaagcctcct gccctaacct
    37441 ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc ctcggcgcta tttgtacccg
    37501 ccgttgtccg ttctgtgatg ttgcccacgg tcgcccggta gctcctgatg ccaatgaacc
    37561 agtgaaactg gcgcagacca ttgccgatat ggcgctgcgt tatgtggtta tcacctccgt
    37621 tgaccgtgat gacctgcgcg atggcggtgc ccagcacttt gcggattgca ttactgccat
    37681 tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg ccggatttcc gtggtcgtat
    37741 ggatcgtgct ctggatattc tgactgcaac gccaccagat gtgttcaacc ataacctgga
    37801 aaacgtaccg cgtatttacc gtcaggtacg gcctggtgca gattacaact ggtcgctgaa
    37861 gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg accaagtctg gtctgatggt
    37921 gggactgggt gaaaccaatg aagaaattat tgaggtaatg cgcgacctgc gccgtcatgg
    37981 tgtgacgatg ttaacgctgg ggcaatattt gcagccaagc cgccatcacc tgccggttca
    38041 acgttacgtt agcccggatg agttcgacga aatgaaagcc gaagcgctgg cgatgggctt
    38101 tacccatgct gcatgcggtc cgtttgtccg ctcttcttac cacgccgatt tgcaggcgaa
    38161 agggatggaa gttaagtaat cctgtaataa ttacttacat ctgtctgata aaaaacccgc
    38221 tttttg
//
LOCUS       DAZOYL010000007        36714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZOYL010000007 DAZOYL010000000
VERSION     DAZOYL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.007"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUOY_000247"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUOY_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0681144.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHUOY_000248"
     CDS             complement(280..873)
                     /locus_tag="ACHUOY_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0681145.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUOY_000249"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUOY_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0681146.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHUOY_000250"
     CDS             1788..2183
                     /locus_tag="ACHUOY_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0681147.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUOY_000251"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUOY_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR0681148.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUOY_000252"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUOY_000252"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR0681149.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUOY_000253"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUOY_000253"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR0681150.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUOY_000254"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUOY_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR0681151.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUOY_000255"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUOY_000255"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR0681152.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUOY_000256"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUOY_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR0681153.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHUOY_000257"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHUOY_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR0681154.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHUOY_000258"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHUOY_000258"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR0681155.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHUOY_000259"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHUOY_000259"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR0681156.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUOY_000260"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUOY_000260"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR0681157.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUOY_000261"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUOY_000261"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR0681158.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUOY_000262"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUOY_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR0681159.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUOY_000263"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUOY_000263"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR0681160.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUOY_000264"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUOY_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR0681161.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUOY_000265"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUOY_000265"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR0681162.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUOY_000266"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUOY_000266"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR0681163.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUOY_000267"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUOY_000267"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR0681164.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUOY_000268"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUOY_000268"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR0681165.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUOY_000269"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUOY_000269"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR0681166.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUOY_000270"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUOY_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR0681167.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUOY_000271"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUOY_000271"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR0681168.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHUOY_000272"
     CDS             complement(26207..26548)
                     /locus_tag="ACHUOY_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR0681169.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUOY_000273"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUOY_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR0681170.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUOY_000274"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUOY_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR0681171.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUOY_000275"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUOY_000275"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR0681172.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHUOY_000276"
     CDS             33227..34570
                     /locus_tag="ACHUOY_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR0681173.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="ACHUOY_000277"
     CDS             complement(34650..34856)
                     /locus_tag="ACHUOY_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR0681174.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="ACHUOY_000278"
     CDS             35181..35735
                     /locus_tag="ACHUOY_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0681175.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..>36714)
                     /gene="ytfI"
                     /locus_tag="ACHUOY_000279"
     CDS             complement(35825..>36714)
                     /gene="ytfI"
                     /locus_tag="ACHUOY_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR0681176.1"
                     /translation="NFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWISNYDSLLT
                     LREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLRGSGFEYEE
                     IPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQAIGDVSLG
                     YALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLGFALKIYGG
                     DERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKIC
                     KKYKSTIEAFKKEQLALPV"
BASE COUNT         8893 a         9840 c         9128 g         8853 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttat
//
LOCUS       DAZOYL010000008        34982 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZOYL010000008 DAZOYL010000000
VERSION     DAZOYL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34982
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.008"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHUOY_000280"
     CDS             complement(<1..815)
                     /locus_tag="ACHUOY_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0681177.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHUOY_000281"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHUOY_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHUOY_000282"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHUOY_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1397..1873)
                     /locus_tag="ACHUOY_000283"
     CDS             complement(1397..1873)
                     /locus_tag="ACHUOY_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR0681178.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHUOY_000284"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHUOY_000284"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR0681179.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="ACHUOY_000285"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="ACHUOY_000285"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR0681180.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="ACHUOY_000286"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="ACHUOY_000286"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR0681181.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="ACHUOY_000287"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="ACHUOY_000287"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR0681182.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="ACHUOY_000288"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="ACHUOY_000288"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0681183.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="ACHUOY_000289"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="ACHUOY_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR0681184.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="ACHUOY_000290"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="ACHUOY_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR0681185.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="ACHUOY_000291"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="ACHUOY_000291"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR0681186.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="ACHUOY_000292"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="ACHUOY_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR0681187.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="ACHUOY_000293"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="ACHUOY_000293"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR0681188.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="ACHUOY_000294"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="ACHUOY_000294"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR0681189.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="ACHUOY_000295"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="ACHUOY_000295"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR0681190.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="ACHUOY_000296"
     CDS             13848..14552
                     /locus_tag="ACHUOY_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0681191.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14823..15470
                     /locus_tag="ACHUOY_000297"
     CDS             14823..15470
                     /locus_tag="ACHUOY_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0681192.1"
                     /translation="MWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLC
                     MYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAG
                     LLFNVEPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNK
                     LLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(15535..16246)
                     /locus_tag="ACHUOY_000298"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="ACHUOY_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="ACHUOY_000299"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="ACHUOY_000299"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR0681193.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="ACHUOY_000300"
     CDS             complement(17484..18245)
                     /locus_tag="ACHUOY_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0681194.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="ACHUOY_000301"
     CDS             18378..18788
                     /locus_tag="ACHUOY_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR0681195.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="ACHUOY_000302"
     CDS             complement(18750..19856)
                     /locus_tag="ACHUOY_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0681196.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="ACHUOY_000303"
     CDS             complement(19867..21000)
                     /locus_tag="ACHUOY_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0681197.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="ACHUOY_000304"
     CDS             complement(20993..22729)
                     /locus_tag="ACHUOY_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0681198.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHUOY_000305"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHUOY_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR0681199.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHUOY_000306"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHUOY_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR0681200.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHUOY_000307"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHUOY_000307"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR0681201.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHUOY_000308"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHUOY_000308"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR0681202.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="ACHUOY_000309"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="ACHUOY_000309"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR0681203.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHUOY_000310"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHUOY_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR0681204.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHUOY_000311"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHUOY_000311"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR0681205.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHUOY_000312"
     CDS             31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHUOY_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR0681206.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHUOY_000313"
     CDS             complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHUOY_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR0681207.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHUOY_000314"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHUOY_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR0681208.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHUOY_000315"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHUOY_000315"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR0681209.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34982)
                     /locus_tag="ACHUOY_000316"
                     /pseudo
     CDS             complement(32997..34982)
                     /locus_tag="ACHUOY_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8273 a         8977 c         9488 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 cc
//
LOCUS       DAZOYL010000009        34948 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZOYL010000009 DAZOYL010000000
VERSION     DAZOYL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.009"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUOY_000317"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUOY_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR0681210.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUOY_000318"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUOY_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR0681211.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUOY_000319"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUOY_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR0681212.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUOY_000320"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUOY_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR0681213.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUOY_000321"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUOY_000321"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR0681214.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUOY_000322"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUOY_000322"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR0681215.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHUOY_000323"
     CDS             complement(6574..7338)
                     /locus_tag="ACHUOY_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0681216.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUOY_000324"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUOY_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR0681217.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHUOY_000325"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHUOY_000325"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR0681218.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHUOY_000326"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHUOY_000326"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR0681219.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="ACHUOY_000327"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="ACHUOY_000327"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR0681220.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="ACHUOY_000328"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="ACHUOY_000328"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR0681221.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUOY_000329"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUOY_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR0681222.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUOY_000330"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUOY_000330"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR0681223.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUOY_000331"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUOY_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR0681224.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUOY_000332"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUOY_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR0681225.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="ACHUOY_000333"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="ACHUOY_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR0681226.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUOY_000334"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUOY_000334"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR0681227.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALREYVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUOY_000335"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUOY_000335"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR0681228.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUOY_000336"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUOY_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR0681229.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUOY_000337"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUOY_000337"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR0681230.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUOY_000338"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUOY_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR0681231.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHUOY_000339"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHUOY_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR0681232.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHUOY_000340"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHUOY_000340"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR0681233.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="ACHUOY_000341"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="ACHUOY_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHUOY_000342"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHUOY_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR0681234.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="ACHUOY_000343"
     CDS             complement(31717..33003)
                     /locus_tag="ACHUOY_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0681235.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="ACHUOY_000344"
     CDS             complement(33054..33995)
                     /locus_tag="ACHUOY_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0681236.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="ACHUOY_000345"
     CDS             complement(34010..34780)
                     /locus_tag="ACHUOY_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR0681237.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         8339 a         9711 c         9160 g         7738 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tatgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcgaatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaaca
//
LOCUS       DAZOYL010000010        32737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZOYL010000010 DAZOYL010000000
VERSION     DAZOYL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.010"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            370..1743
                     /gene="aroP"
                     /locus_tag="ACHUOY_000346"
     CDS             370..1743
                     /gene="aroP"
                     /locus_tag="ACHUOY_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR0681238.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1786..2640)
                     /gene="ampE"
                     /locus_tag="ACHUOY_000347"
     CDS             complement(1786..2640)
                     /gene="ampE"
                     /locus_tag="ACHUOY_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR0681239.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2637..3188)
                     /gene="ampD"
                     /locus_tag="ACHUOY_000348"
     CDS             complement(2637..3188)
                     /gene="ampD"
                     /locus_tag="ACHUOY_000348"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR0681240.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3276..4169
                     /gene="nadC"
                     /locus_tag="ACHUOY_000349"
     CDS             3276..4169
                     /gene="nadC"
                     /locus_tag="ACHUOY_000349"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR0681241.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4372..4812
                     /gene="ppdD"
                     /locus_tag="ACHUOY_000350"
     CDS             4372..4812
                     /gene="ppdD"
                     /locus_tag="ACHUOY_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR0681242.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4822..6208
                     /gene="gspE"
                     /locus_tag="ACHUOY_000351"
                     /pseudo
     CDS             4822..6208
                     /gene="gspE"
                     /locus_tag="ACHUOY_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            6198..7400
                     /gene="hofC"
                     /locus_tag="ACHUOY_000352"
                     /pseudo
     CDS             6198..7400
                     /gene="hofC"
                     /locus_tag="ACHUOY_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(7435..8478)
                     /gene="guaC"
                     /locus_tag="ACHUOY_000353"
     CDS             complement(7435..8478)
                     /gene="guaC"
                     /locus_tag="ACHUOY_000353"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR0681243.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8634..8678)
                     /locus_tag="ACHUOY_000354"
     CDS             complement(8634..8678)
                     /locus_tag="ACHUOY_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681244.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8703..9323
                     /gene="coaE"
                     /locus_tag="ACHUOY_000355"
     CDS             8703..9323
                     /gene="coaE"
                     /locus_tag="ACHUOY_000355"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR0681245.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9323..10066
                     /gene="zapD"
                     /locus_tag="ACHUOY_000356"
     CDS             9323..10066
                     /gene="zapD"
                     /locus_tag="ACHUOY_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR0681246.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            10076..10273
                     /gene="yacG"
                     /locus_tag="ACHUOY_000357"
     CDS             10076..10273
                     /gene="yacG"
                     /locus_tag="ACHUOY_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR0681247.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10373..10762)
                     /gene="mutT"
                     /locus_tag="ACHUOY_000358"
     CDS             complement(10373..10762)
                     /gene="mutT"
                     /locus_tag="ACHUOY_000358"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR0681248.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10822..13527)
                     /gene="secA"
                     /locus_tag="ACHUOY_000359"
     CDS             complement(10822..13527)
                     /gene="secA"
                     /locus_tag="ACHUOY_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR0681249.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13589..14101)
                     /gene="secM"
                     /locus_tag="ACHUOY_000360"
     CDS             complement(13589..14101)
                     /gene="secM"
                     /locus_tag="ACHUOY_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR0681250.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14332..15249)
                     /gene="lpxC"
                     /locus_tag="ACHUOY_000361"
     CDS             complement(14332..15249)
                     /gene="lpxC"
                     /locus_tag="ACHUOY_000361"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR0681251.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15350..16501)
                     /gene="ftsZ"
                     /locus_tag="ACHUOY_000362"
     CDS             complement(15350..16501)
                     /gene="ftsZ"
                     /locus_tag="ACHUOY_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0681252.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16562..17824)
                     /gene="ftsA"
                     /locus_tag="ACHUOY_000363"
     CDS             complement(16562..17824)
                     /gene="ftsA"
                     /locus_tag="ACHUOY_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR0681253.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17821..18651)
                     /gene="ftsQ"
                     /locus_tag="ACHUOY_000364"
     CDS             complement(17821..18651)
                     /gene="ftsQ"
                     /locus_tag="ACHUOY_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR0681254.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18653..19573)
                     /gene="ddlB"
                     /locus_tag="ACHUOY_000365"
     CDS             complement(18653..19573)
                     /gene="ddlB"
                     /locus_tag="ACHUOY_000365"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0681255.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19566..21041)
                     /gene="murC"
                     /locus_tag="ACHUOY_000366"
     CDS             complement(19566..21041)
                     /gene="murC"
                     /locus_tag="ACHUOY_000366"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR0681256.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21095..22162)
                     /gene="murG"
                     /locus_tag="ACHUOY_000367"
     CDS             complement(21095..22162)
                     /gene="murG"
                     /locus_tag="ACHUOY_000367"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR0681257.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22159..23403)
                     /gene="ftsW"
                     /locus_tag="ACHUOY_000368"
     CDS             complement(22159..23403)
                     /gene="ftsW"
                     /locus_tag="ACHUOY_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR0681258.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23403..24719)
                     /gene="murD"
                     /locus_tag="ACHUOY_000369"
     CDS             complement(23403..24719)
                     /gene="murD"
                     /locus_tag="ACHUOY_000369"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR0681259.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24722..25804)
                     /gene="mraY"
                     /locus_tag="ACHUOY_000370"
     CDS             complement(24722..25804)
                     /gene="mraY"
                     /locus_tag="ACHUOY_000370"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR0681260.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25798..27156)
                     /gene="murF"
                     /locus_tag="ACHUOY_000371"
     CDS             complement(25798..27156)
                     /gene="murF"
                     /locus_tag="ACHUOY_000371"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR0681261.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27153..28646)
                     /gene="murE"
                     /locus_tag="ACHUOY_000372"
     CDS             complement(27153..28646)
                     /gene="murE"
                     /locus_tag="ACHUOY_000372"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR0681262.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28633..30399)
                     /gene="ftsI"
                     /locus_tag="ACHUOY_000373"
     CDS             complement(28633..30399)
                     /gene="ftsI"
                     /locus_tag="ACHUOY_000373"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR0681263.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30415..30780)
                     /gene="ftsL"
                     /locus_tag="ACHUOY_000374"
     CDS             complement(30415..30780)
                     /gene="ftsL"
                     /locus_tag="ACHUOY_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR0681264.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30777..31718)
                     /gene="rsmH"
                     /locus_tag="ACHUOY_000375"
     CDS             complement(30777..31718)
                     /gene="rsmH"
                     /locus_tag="ACHUOY_000375"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR0681265.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31720..32178)
                     /gene="mraZ"
                     /locus_tag="ACHUOY_000376"
     CDS             complement(31720..32178)
                     /gene="mraZ"
                     /locus_tag="ACHUOY_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR0681266.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7793 a         9176 c         8061 g         7707 t
ORIGIN      
        1 gcacaaatgt agactttacg taggaaaggg attttttaac gattattaaa ctataaaaat
       61 ccgaattgaa ccgattcact taccaatttt ttgattttta agtcaataaa aacgaattta
      121 aattcattct gcatattgag aggggttgag gctgagcttt acaaacggtt tctttttaag
      181 caactcatct tcaaccatgc ataaagcggg tgcattcgct gccgcatacc attattcttg
      241 atctgacgga agtctttttg taacaattca aacttctttg atgtaaaaaa attaatacaa
      301 caaacggaat tgcaaactta cacacgcatc actgcgtaga tcaaaaaaac aatcaccgca
      361 cgaggtttca tgatggaagg tcaacagcac ggcgagcagc taaagcgcgg ccttaaaaac
      421 cgccatattc agcttatcgc gctgggtggc gcgataggga cagggctatt cctgggtagc
      481 gcctccgtaa tacagtccgc agggccaggg attatcctgg gttacgccat tgctggtttt
      541 atcgcctttc tgatcatgcg tcagctgggt gaaatggtgg tcgaagaacc tgtcgcaggc
      601 tcctttagcc actttgctta taaatactgg ggcagttttg ccggtttcgc ctctggctgg
      661 aactactggg tactgtacgt tttagttgcc atggcagagc tgactgccgt gggtaaatac
      721 attcagttct ggtatccgga aatcccaacc tgggtttctg ccgccgtatt ctttgtggtg
      781 attaacgcca tcaacctgac caacgttaaa gtgtttggcg agatggagtt ctggtttgcc
      841 attatcaaag ttattgcgat agtagcgatg atcatcttcg gcggctggct gctgttcagt
      901 ggcaacggcg gcccgcaggc gagcgttagc aacctgtggg atcaaggtgg tttcctgccg
      961 cacggcttca ccggactggt gatgatgatg gcgattatca tgttctcgtt cggtggtctg
     1021 gaactggtgg ggatcaccgc agcagaagct gataacccgg agcaaagtat cccgaaagcg
     1081 actaaccagg ttatctaccg catcctgatt ttctatattg gttcgttagc cgttctgctc
     1141 tcactgatgc cgtggacccg cgttaccgcc gataccagtc cgtttgtgct gatcttccac
     1201 gagttaggcg atacctttgt ggcgaatgcg ctgaacatcg tggtactgac tgcggcgctc
     1261 tccgtgtaca acagctgcgt atattgcaac agccgtatgc tgtttggtct ggcacaacag
     1321 ggtaatgcgc caaaagcgct ggcgtctgtc gataaacgcg gcgtaccggt taacaccatt
     1381 ctggtgtctg cgctggttac agcattgtgc gtattgatta actatcttgc tccggaatcc
     1441 gcatttggcc tgttaatggc actggtggta tccgcactgg tgatcaactg ggcgatgatc
     1501 agtctggcgc atatgaagtt ccgtcgcgcc aagcaggaac aaggcgtggt aactcgcttc
     1561 cctgctctgc tttatccgct gggtaactgg atctgcctgc tgtttatggc ggcggtactg
     1621 gtgattatgc tgatgacccc aggaatggcg atttcggtat acctgatccc ggtatggctg
     1681 atcgtgttag gtatcggcta tctgtttaaa gagaaaaccg ccaaagccgt aaaagcgcat
     1741 taatctctct acgccctcac ccatgcaggg tgagggcaat aatctttaca ccaacgctcc
     1801 gtaaatcgtc agtagcgcaa tcaccaccac gaccacgaac gaggttttct tcgccattga
     1861 aaccgctgcc ttcggcgtct ccaccttatc gacatgcggt tcacgcgcca gagagaactg
     1921 cgccagacgc gttaacacct gatactgcga agtatggaag tcacccagcg aagcaaacca
     1981 ggccggtaac gctttctccc catgaccgat caaggcatat accacacccg caagacgaac
     2041 cggcacccaa tccagtacat gaagcacggc atcaatgccg gactgtaaac gatgatgcgg
     2101 cgtctggtat cgcgccagcc agtattgcca tgcacgcaaa aacgcatacc ccatcagcgt
     2161 aacgggtccc caggttcccc ccacaatcaa ccagaacagc ggtgcaagat aaaaacgaaa
     2221 gttaatccac agcaatgcat tttgcagctc acgcaaatac tcacgttcgt cgcagcctgc
     2281 cgggacgccg tgaatcatgg tgagttcgcc agccatcgtg gcacgggcat ggctatcatt
     2341 acgtgaagca gctgtcagat aagcatgata atgaagacga actttacctg cgccaataca
     2401 cagcaaacca atcagcagcc acaccagcag cgtgggaacg ttgaacaata ctccctgcaa
     2461 tgcgcgtaac agtaaaaaag tcacgcccat cgcaataatg gtcatgccta acgtgcgccc
     2521 gagagaaaaa tgtttcaccc gccgaaagaa cgcttcaaga cgatgatcaa gctgccagtg
     2581 ctcgcccaac ttaaacaggc gctcgaaaat taacaccagt aaggttgtaa atagcgtcat
     2641 gttgtctcct tgctgaccag cgcacgaaac cgtgcccaat caaatgcagg accgggatcg
     2701 gttttccgat ccggcgcaat atcacaatgg cccgtcatgt tattagcgat atcgggatag
     2761 cgatctatca gtgcccgcgt aaccgccgca agctgttgat actgcgcatc ggtatacgcc
     2821 agagtatcgg tgccttcaag ctcaatccca atagaaaaat cattgcagcg ttcacgcccc
     2881 tgatactgag agactcccgc atgccaggca cgtttatcga aaggaacata ctggactatt
     2941 tcaccatcac ggcgaatcaa acagtgagcg gagacgcgca aatgggcgat ctcagcaaag
     3001 aaaggatgtg cctgcggatc aatagttcca gtgaataatg cgtcgatcca cggaccgcca
     3061 aactcgcctg gcggcaggct aatattgtgc accaccagca gggtgggtgt ttcgtcatcc
     3121 gggcggcaat cgtaatgtgg tgagggaacg cggcgcgcgc caaccagcca cccctgttct
     3181 aacaacatgc aggatctcct tatatgtggt gctaataccc ggttcagagt agcatgtttc
     3241 taccttatga ttcgttagct atctggagtt ttaacatgcc gcctcgccgc tataaccctg
     3301 acacccgacg tgacgagctg ctggaacgca ttaatctcga tatccccggc gcggtggccc
     3361 aggcgctgcg ggaagattta ggcggaacag tcgatgccaa caatgacatt acggcaaaac
     3421 ttttaccgga aaactctcgc tctcatgcca cggtgagcac ccgcgagaat ggcgtatttt
     3481 gcggcaaacg ctgggttgaa gaggtgttta ttcaactggc aggcgacgat gtcaccataa
     3541 tctggcatgt ggatgacggc gatgtcatca acgccaatca acccttgttc gaacttgaag
     3601 gcccatcccg cgtgctgtta acgggcgaac gcactgcgct taattttgtg caaacccttt
     3661 caggagttgc cagtaaggta cgccactatg tcgaattact ggaaggcacc aacacgcagt
     3721 tgttggatac gcgcaaaacc ttacctggcc tgcgttcagc tctgaaatac gcggtacttt
     3781 gcggcggcgg agcgaatcac cgtctggggc tttctgatgc cttcctgatc aaagaaaatc
     3841 atattattgc ctccggctca gtgcgccagg ccgtcgaaaa agcgtcctgg ctgcacccgg
     3901 atgcgccagt agaagtcgaa gtagagaatc tggaagaact tgatgaagcc ctgaaagcag
     3961 gagccgatat catcatgctg gataacttcg aaacagaaca gatgcgcgaa gccgtcaaac
     4021 gcaccaacgg caaggcgcta ctggaagtgt ctggcaacgt cactgacaaa acactgcgcg
     4081 aatttgccga aacgggcgtg gactttatct ccgtcggtgc gctaactaaa cacgtacaag
     4141 cactcgacct ttcaatgcgt tttcgctaat tgctgacaga tttatccgct ccatcgcggg
     4201 cggataatct ccccttcccc aactttcttc gtaacgcctc gcaaatttct tgtcttttgc
     4261 tgcaacgaaa taaaaaacct cggaagctgc tttcccgttt cgttttttgc ccctcgcttg
     4321 tgctgatggc gtttgccaaa gtagcgccaa ccaaatcaag gagcgaaaca gatggacaag
     4381 caacgcggtt ttacacttat cgaactgatg gtggttattg gcatcattgc cattttaagc
     4441 gccattggta ttcccgctta tcaaaactac ctgcgcaaag ctgcactcac cgacatgcta
     4501 caaacctttg tgccttaccg tacagccgta gagttgtgcg cgctggaaca tggtggatta
     4561 gatacctgcg acggtggcag caatggcatt ccctcgccta ccaccacccg ctatgtttca
     4621 gccatgagtg tggcaaaagg cgtggtgtcg ctgaccgggc aagaaagtct caatggacta
     4681 agcgtcgtca tgacaccggg ttgggataac gcaaacggcg tcaccggctg ggcgcgcaac
     4741 tgcaatattc aaagtgacag cgcattgcag caagcctgcg aagatgtctt ccgctttgat
     4801 gacgccaact aaggagcggc aatgaatatt ccacagctca ccgccctgtg tctgcgttat
     4861 cagggagtct tgctggatgc cagcgaagag gtggttcatg ttgcggtagt cgatgcacct
     4921 tcgcatgagt tgctggacgc attgcatttc gctaccacca aacgtattga gatcacctgc
     4981 tggacgcgcc aacaaatgga aggccacgcc agtcgcacac aacagacatt gcccgtagct
     5041 gttcaggaga agcatcagcc caaagcagag ttgctgactc gaacgttaca atctgcgctg
     5101 gaacaacgtg cgtctgatat tcatatcgaa ccagcggaca atgcctaccg catccgcttg
     5161 cgtatcgacg gcgtattgca tcctttaccg gatgtttcac cggatgccgg agtcgcatta
     5221 accgccagat taaaagtgct gggaaacctg gatattgcgg aacatcgcct gccgcaggac
     5281 gggcaattca ctgtcgaact ggcaggaaac gccgtctcat ttcgtattgc gaccttacca
     5341 tgtcggggtg gtgaaaaggt ggtattaagg ttgttacagc aggtgaacca ggcactggat
     5401 gttaacacgc tgggaatgca gccgttacaa ctggcggact ttgctcatgc cttgcaacaa
     5461 ccacagggac tggtgctggt aactggccct acaggcagcg gcaaaacggt cacgctttat
     5521 agtgccctgc aaaagctgaa taccgctgac attaatattt gtagcgtcga agatccggtt
     5581 gagatcccca tagccggact aaaccagacg caaatccatc cgcgtgccgg gctcaccttt
     5641 cagggcgttt tgcgtgcgtt attgcgccag gatcctgacg tcatcatgat cggagagatc
     5701 cgctatggct ttaacggcag aaattgccat taaagccgcg caaaccggtc acctggtgtt
     5761 gtctacccta cacactaatt ccacctgcga aacgctggta cgtttacagc aaatgggagt
     5821 cgcccgctgg atgctctcat cagcgcttac gctggtaata gcccagcgtc tggtacgtaa
     5881 actttgccca cattgtcgcc agcagcaagg ggagcccatc catattccag tcaatgtatg
     5941 gccgtcgccg ctgccccact ggcaggcacc cggttgtgta cattgctacc acggttttta
     6001 tggtcgtacg gccttatttg aagttctgcc cataacgccg gtcattcgtc agcttatttc
     6061 cgctaatacc gacgttgaat cgctggaaac gcacgcacga caggcgggta tgcgtacgct
     6121 ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc acctttgaag agttaatccg
     6181 cgtactgggg atgccgcatg gcgagtaagc aactctggcg ctggcatggc ataaccggcg
     6241 acggcaatgc gcaagatggg atgctatgag cagagagccg tgctttgctg ctcatggcac
     6301 tacagcaaca gatggttacc ccacttagcc tgaagcgaat cgccatcaat tctgcgcagt
     6361 ggcgaggaga taaaagcgcg gaagtcattc atcaactggc gacgctactc aaagccgggt
     6421 taacgctttc tgaagggctg gcactgctgg cggaacagca tcccagtaag caatggcaag
     6481 cgttgctgca atcgctggcg cacgatctcg aacagggcat tgctttttcc aatgccttat
     6541 taccctggtc agaggtattt ccgccactct atcaggcgat gatccgcacg ggtgaactga
     6601 ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca gcaaaaagcc cagcgtcagt
     6661 tgaccgacaa agtgaaatca gcgttacgtt atcccatcat cattttagcg atggcaatca
     6721 tggtggttgt ggcaatgctg cattttgttc tgccggagtt tgccgctatc tataagacct
     6781 tcaacacccc actaccggca ctaacgcagg ggatcatgac gctggcagac tttagtggcg
     6841 aatggagctg gctgctggtg ttgttcggct ttctgctggc gatagccaat aagttgctga
     6901 tgcgccgacc gacctggctt atagtgcggc agaaattgct gttacgcatc ccgattatgg
     6961 gttcactgat gcggggacaa aaactcacgc agatctttac gattctggcg ctgacacaaa
     7021 gtgcaggcat tacttttttg cagggcgtag agagcgtcag agaaacaatg cgctgcccgt
     7081 actgggtgca acttctgaca caaatccagc acgatatcag taacggtcat cccatctggc
     7141 tggcgctaaa aaataccggg gagtttagcc cgctctgttt gcaattagtg agaacaggag
     7201 aggcatccgg ctcgctggac ctcatgttag acaacctcgc ccatcatcat cgggataaca
     7261 caatggcgct ggcggataac ctcgcagcct tactggaacc gacgttgctg atcataacgg
     7321 gaggaattat cggtacgctg gtggtggcga tgtatctgcc aattttccat ttaggcgatg
     7381 cgatgagtgg gatgggataa cgctggcgtg ttgctccaca ccagcgttgg aagattacag
     7441 gttgttgaag atgcggtttt cttgttcctg cacacggata aatgtggtgc gcttggtcag
     7501 ttctttcagg cgtgaagccc caacgtatgt acaagctgaa cgcaggccgc ccaaaatatc
     7561 acgcgcggta ttttcaaccg ggcctcgcag cggcagctta acggttttac cttctgctgc
     7621 gcgatattcc gcaacgccgc caacgtgacg tttcatcgca gactcggagc tcatgccgta
     7681 gaacagcata aatttctcgc cgttctcctc aacgatgcga ccgccgctct cttcgtggcc
     7741 cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg ccaaaggctt ttgcgacatc
     7801 gcccggcgtg gtgcagccac cgtcgctgac gatcattccg cccagaccgt gcgccgcatc
     7861 ggcacattcg attaccgcag aaagctgcgg ataaccgacg cctgttttga cgcgagttgt
     7921 acaaacagaa cctgggccaa tgccaacttt aacgatatca gcaccggaga ggataagctc
     7981 ctcacacatt tcaccagtca ctacgttgcc agcacaaatg gttttggtcg gccacgcttc
     8041 acgcgctttc gcaacgaact gcacgaagtg ttcggaataa ccattcgcca catcaataca
     8101 aacgaagttt aatgccgggt tcaggtcgag aatctgttta gttttttcga aatccgcatc
     8161 agacgtaccg gtagaaacca tcacatgttt cagcacatca gcggaagaat tgttgataaa
     8221 cgcttgccac tcttcgacag aatagtgttt atgcacagca gtcaaaatat caaaagaagc
     8281 cagcgcagag gccatagaaa atgtacctac ggtgtccata tttgcggcga taatcggcac
     8341 gccggaccag ctctgacctg aatgtttgaa ggtgaattga cgttccagtt caacatcgga
     8401 acggctttta agagtggagc gtttagggcg gatgagaacg tctttaaaac ctaacttcag
     8461 atcttcttca atacgcatgt gcggattcct ggggttaatg gctataaggg taagagcgca
     8521 actccagtga cgctatcata cgcactaatc aatgcggcgc aagactgcga aattgtcttt
     8581 ttttacgcta caatcccata aatttacctg gtaaatagtg agcgaacgtt tcatcaattt
     8641 cgctttcgtg cactcatttt taacgttttg ataatcatga tgaattcccg ggaatataaa
     8701 ttatgaggta tatagttgcc ttaacgggag gcattggcag tggcaagagt accgttgcca
     8761 atgcgtttgc tgatctcgga attaacgtca ttgatgccga tattattgcg cgtcaggtgg
     8821 ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca ctttggcgct aacatgattg
     8881 ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg gatcttcgcc aacccggaag
     8941 agaaaaactg gcttaacgcc ctgctgcatc cgctgattca gcaagagacg caacaccaga
     9001 tccagcaagc tacttccccc tatgtactgt gggttgtgcc attgctggta gaaaactcac
     9061 tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag cccagaaacg caacttaagc
     9121 gcaccatgca gcgcgatgat gtaactcgcg agcatgtcga acaaatcctt gctgctcagg
     9181 caacgcgcga agcccgcctt gccgtggcag atgacgtcat tgataataac ggcgcaccgg
     9241 atgctatcgc atcggatgtt gcccgcctgc acgcacacta tttgcagctt gcgtcgcagt
     9301 ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt tttgaacatc cactaaatga
     9361 aaaaatgcgt acatggctgc gcattgagtt tttgattcag caactcaccg ttaatttacc
     9421 catcgttgac cacgctggcg cgctgcattt cttccgtaat gtcagtgaat tactggatgt
     9481 tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa cttgaccggc agcaacgtaa
     9541 actccagacc tggattggcg tgcctggcgt ggaccagagc cgtattgaag cattaattca
     9601 gcagttaaaa gcggcgggga gcgtattaat ttccgcgccg cgtatcgggc aatttctgcg
     9661 tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc atcccaggcg gctgttgcag
     9721 ctttgattta cctacattgc acatttggct gcatctaccc caggcgcagc gcgacagcca
     9781 ggtagaaacc tggattgcca gcctgaaccc gctcacccag gcacttacca tggtgctgga
     9841 tttaattcgc cagtcggccc ccttccgtaa acaaaccagt ctgaatggtt tttatcagga
     9901 taacggtggc gatgccgact tgctgcgcct gaatctgtcg ctcgattcac agctttatcc
     9961 gcaaatttcc ggacataaga gccgttttgc cattcgtttt atgccgctgg acactgaaaa
    10021 cggacaggta ccggaacgtc tggatttcga actggcctgt tgctaaggag taaaaatgtc
    10081 agaaactatt acggtgaatt gcccaacctg cgggaaaacg gtggtgtggg gtgaaatcag
    10141 cccgtttcgg ccattttgct ccaaacgttg tcagctgatc gacctcggag aatgggctgc
    10201 tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa agcgatgact ggagcgaaga
    10261 gccaaagcag tgacatttgt ctgatgccgc acgtaggcct gataagacgc ggacagcgtc
    10321 gcatcaggca tcttgtgcaa atgtcggatg cggcgtgaac gccttatccg acctacagac
    10381 gcttaagctt cgcaattacc ggttcattgg ctggcggaaa atcatcggca ttaagaccga
    10441 ctagcgacat ccactcaccg ggttgccctt ctttacccca cggctcccct tcccagcttt
    10501 cgaccagcca gaaccacaga gttatatgcc tgtccgggaa ttcatattcc agtttttcaa
    10561 atagcgaaaa atgttgtggg gtaatcccga cttcttcctg aagttcacgc actaccgcct
    10621 gttccggcgt ttcgcccatt tcaattttac cgccgggaaa ctccagttta ttcgccatgt
    10681 gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt ctcgttgcga ataataccta
    10741 ccgcaatttg cagctttttc attgtcttaa acctataaaa aaggcgcaga atcctgcgcc
    10801 ttttacttca actgttagct tttattgcag gcgaccatgg cactgcttgt attttttacc
    10861 agaaccgcac gggcaaggat cgttacgtcc tactttgcgc tcaccggttt gcgccgccag
    10921 tgcagctgcg gctgcagagt cgtcatcctg atggctaagc tgctgcattt gcgctaaacg
    10981 ctcggcttcc atacgacgct gttgttccag ctcctcaacc tcttcaggca tacgtacctg
    11041 gactttgctc agcgtgctga taacttcata ttttaacgac tccagcatcg ctgcaaacat
    11101 ggagaacgat tcacgtttgt attcctgctt cggatctttc tgtgcatagc cacgcaggtg
    11161 gataccctga cgcagatagt ccatcgccgc taggtgctct ttccacagag agtcgagagt
    11221 ttgcagcatg acgccttttt cgaagtgacg catcatctca gcaccaacca cttcttcttt
    11281 acgctgatac acttcgatgg actgcgccag aattcgctca cgcagcgtct cttcatgcag
    11341 ttctggttct ttatccagcc actcggcaat tggcaaatcg aggtcgaaat cgttcttcag
    11401 acgttcctgc agccccggaa tatcccacat ttcttccagt gactgtggcg gaatgtaggc
    11461 atcaatggtc gctttgaaca catcttcacg aatgctgttg atggtttcgc tcacatcgct
    11521 gacatccagc agttcgttac gctgggagta aatggcgcga cgctgatcgt tagccacgtc
    11581 atcatattcc agcagttgct tacgaatgtc gaagttacgg ctttcaactt tacgctgggc
    11641 gttggcaatc gctttagtca cccacgggtg ttcaatggct tcgcctggct tcatacccag
    11701 tttacgcatc atgccggata ctcggtcgga agcaaaaata cgcatcagcg catcttccat
    11761 cgacaggtag aaacgggaag aaccagcatc cccctgacga ccagaacgac cgcgcaactg
    11821 gttatcgata cgacgggatt cgtgacgctc ggtaccgatg atatgcaggc cacctgcttc
    11881 cagtaccgca tcgtgacgta cctgccagtc ggctttaatt ttttcaattt gctctgcggt
    11941 cggattttcc agcgcggcaa cttctgcctg ccagctacca ccgagcacaa tatctgtacc
    12001 acgacccgcc atattggtcg cgatagtcac cgcagccgga taacctgcct gagcaacaat
    12061 cgccgcttcg ttggcgtgga atttggcgtt caggacgttg tgcttaatac cggctttggt
    12121 cagttcgttt gacaccagct ccgatttttc gatggagata gtacccacca gcaccggctg
    12181 gcctttcgca gtacgttctt tgatatcttc aatgatcgcc tgaatttttt ccgcttcagt
    12241 catgtagacc aggtccggca gatctttacg aatcattgga cggttggtcg gaacaacgac
    12301 ggtatccagc ttgtagatgg agctaaattc gaaagcttcg gtatcagcag taccggtcat
    12361 ccccgccagt ttttcataca gacggaagta gttctggaag gtgatcgaag ccagcgtttg
    12421 gttttcgttc tggatctgca caccttcttt cgcttccaca gcctggtgca gaccatcgga
    12481 ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca acgatgataa cttcaccatc
    12541 tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga gcgcgcagcg ccgccgttac
    12601 gtggtgcatc agcatgatgt tggccggaga gtacagagac tccccttcat ccatgatgcc
    12661 ctctttcacc agcagttctt caatcagcac cagaccacgt tcggtcaggt tcacctggcg
    12721 agatttttcg tccaccgaga agtggccttc gccctggaag gtttcggagt cttctttttc
    12781 ctgacggatc aggtgcggaa taattttatt cacgcgttta tacatttccg agctgtcttc
    12841 tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg atcaggatgg agtccacttc
    12901 gtccaccagc gcatagtgca gtttacgctg tacacgttct tcagggctga acgccatatt
    12961 gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg taagtgatgt cagctgcgta
    13021 agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg ataccgacag tcaggccaag
    13081 gaattcaaac agcggacggt tgttttcggc gtcacgttgc gccaggtagt cgttgacggt
    13141 aactacgtgc acgcctttac cggttagtgc gttcaggtaa gcaggcagcg ttgcggtcag
    13201 ggttttacct tcaccggtac gcatttcggc gatgcagcgt tcgttaagaa ccataccgcc
    13261 gagtaactga acgtcgaagt gacgcatacc aaagacgcgc ttacttgcct cacgtaccac
    13321 ggcgaaagct tccgggatca gattttccag cacttcgcct ttttccagac gtgcacgaaa
    13381 ctctgcggtt ttccctttca gttcttcgtc ggagagtttt tccatctccg gttccatggc
    13441 attgatgatg ttgaccactt tgcgcatccg gcgcagggtg cgatcgttac gactaccgaa
    13501 aactttagtt aacaatttga ttagcataat aaaatctcaa acgccccgcg ttgcggagtt
    13561 aataaaatga agtaaaggtt tattgttgtt aggtgaggcg ttgagggcca gcacggatgc
    13621 cttgcgcctg gcttatccag acgggcgtgc tgaattttgc ttgtggggta aaatgcgcat
    13681 aatcaatgcg ataacccttt tcagacggcg tgccttcctg ggtcagcagc gcgctgagcg
    13741 tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga ctcttcagca acgggcagtg
    13801 tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt gcgaatggca tgttgatgcc
    13861 agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt cgcttccagc aaggccaatt
    13921 gaccaaagtt aactttggct gaaggctcgt ggttgcgggt tgtcgctttt gcgggcgcgt
    13981 ttggttcggc ggcgttgctg agcgcaggca aacctaaact cgccgcaacc atccctaata
    14041 agagatgcgg ccagaagtag cgtttaccaa actgtcgcca gcgcgtcagt attccactca
    14101 cgttattgcc atcccgatcg tattcaaacg tatttagcgc acaaatctta tcattaaagc
    14161 ctgcagtcca cagcacgaat gaagataagt tgtgcggaaa agtgcttaag aaaacagcgt
    14221 tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa atgactggtt cacctggccg
    14281 gagagagtgc cagatttgcc agtcgaattt tatacgacag tataaatgtc gttatgccag
    14341 tacagctgaa ggcgctttga aggccaacgg cagttctgcg tcgtcctgga aggtcacata
    14401 ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt ttgttattca gtgcatgacc
    14461 ggatttataa gcggtaaatg caccaataat attgtgacca cacatgaaca agtcaccgat
    14521 cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa cgcaggccgt cttcgttcag
    14581 tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg ccgcccaggc acaaaccacg
    14641 ggactgcaga tattcgatat cacgcatgaa accgaacgta cgcgcacggc tgatctggcg
    14701 cataaacgca tcagcggaga agttcatcgc atagcgctgg ttgctggaat caatagccgg
    14761 atggttaaaa tcgatggtga aatccagcga aaaaccattg tacggcttaa attcagccca
    14821 cttatcgcca tcttcgacac gaacagtctc tttgatgcga acaaattttt tggcgcagtt
    14881 caactcgtcg ataccggcgt caagcagcag gtatacaaac ggagcggcgc tgccgtccat
    14941 gatcgggatt tccggcgcgt taacttcgat aacaatgtta tcgatgccca ggcccgcgag
    15001 agcagcattg aggtgctcta cggttgaaat ccgtacatca tgctcgttga ccagacacgt
    15061 acagagcatg gtatcacgca cagatttggc atcggccggg aaatctaccg gtggattcaa
    15121 gtcggtgcga cgatagatga ccccggtgtt ggccggcgca gggcgtaacg tcagagtgac
    15181 tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg atacgtttaa gtgtcctttg
    15241 tttgatcatc gtattatctc gccaaattac ctatccaacc gaagtgtact atacattcgg
    15301 cgggccagtt tagcacaaag agcctcgaaa cccaaattcc agtcaattct taatcagctt
    15361 gcttacgcag gaatgctggg atatccagat aatccggctc tttcgcagtt tgcggcgcat
    15421 tgtcattcac gactttagca accggcttct gctcctgggt cagcggagcc atcccatgct
    15481 gctggtagcg atccatcact ggctgctgaa cctgcttatt ggtcaccaga gtgatttcag
    15541 gacgtttgtc catgccgata cctgtcgcaa caacggttac gcgcagctcg tcattcatat
    15601 ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc ggaagcaaat gcacggatgg
    15661 tgttacctac cgtttcgaac tcatccagac gcaggtcgaa gcccgccgtg atgttaacca
    15721 gcacgccgcg cgcgccagac aggtcgatat cttccagcag cggagaagag atagccattt
    15781 cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc agaacccatc attgcgtagc
    15841 ccatctcaga cattacggtg cgtacgtctg caaagtccac gttcatcaaa cccggacgag
    15901 taatcagttc agcgatacct tgcacagcgc ctttcagtac atcgttcgct gcgccaaacg
    15961 catccagcag ggagataccg cggcccagaa ctttcagcag tttgtcgttc gggatagtga
    16021 tcagagagtc cacatgcttg gacagttcag tgatcccctg ctccgcgaat gccatacgct
    16081 tcttgccttc aaagttgaaa ggcttagtga cgacagcaac ggtcaggata cccaaatctt
    16141 ttgccacttc agcgacgact ggtgctgcac ctgtaccggt accaccaccc atacccgcag
    16201 caataaagac catgtctgca ccttccagcg ccgcacgcaa tgcatcgcga tcctcatcag
    16261 ccgcattgcg accaacttct ggattagcgc cagcgcccag tcctttggtg ataccgctac
    16321 cgatttgaat cgtctgtcca accgctgttt tacgcagcgc ttgtgcatcg gtatttaccg
    16381 cgaagaattc aacaccttca atacgctcgc gcaccatgtg ttcaacagca ttaccgccgc
    16441 cgccgccgac gccgatgact ttaatcaccg cgtcattggt aagttccatt ggttcaaaca
    16501 tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa tcatcgtcgg cctcataaaa
    16561 attaaaactc ttttcgcagc caactattga gtcgcttgat ccacgagcca actgatgctg
    16621 taacacgttt ttctacttca gcttcaccgt taagatgtga ctctttccca tagtgaagca
    16681 atcccaccgc cgtcgaataa tacggctcct gagcataatc cgttaaaccg gtaatgttca
    16741 gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg agcacaggct gcaagacctt
    16801 cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc caggtgatgt ttaacccctt
    16861 gttggcgaag cttttcctgc aactgcaata tctcttcgtt gaccaggttg agcagctcgg
    16921 tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg cagactccgt ggcggacgac
    16981 cgcctacgct cggcacttcc acgctctcat cttttccaac gatggaaccc agcgcacaac
    17041 cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt gccaaaggcg taagcgatat
    17101 cactggtcac gacattgcca gcataaggaa ttaccttagt gtggcgcaat gccccaccgg
    17161 tataaacggc gatatccatt gtaccaccac cgatatcgac gacgcagaca cccagttcac
    17221 gttcatcttc cgtcaatacc gaataacttg atgccagtcc ggcaaatatc agttggtcaa
    17281 ctttcagccc acaacgttca accgctttga cgatgttttt cgccatatcg ttgtgacatg
    17341 tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag tcctaccgga ttcttgatcc
    17401 cttcctgata gtcaatcgca tactcttgcg ggatcacatg cagcacacga tgttcatcgc
    17461 gcacacgcac cgatttcgcg gtatggacga cgttttccac atcttcttgc gtcacttctt
    17521 cttcagaaat aggcaccata ccaatttcat tctggcagct gatgtgctta ccagaaagcg
    17581 ccagatatac cgaagagatc tgacaatctg ccatcaattc tgcctggtca atggcgcgtt
    17641 gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc tttatccata ccacgtgacg
    17701 ggcagctgcc cacgccaatg atattgacca taccgtcggg cagaacttcc cctactaaag
    17761 cggcaacctt cgcggtacca atctccagtc ctactaccag ttttctgtcc gtcgccttga
    17821 tcattgttgt tctgcctgtg cctgattttg ttgctgagta gattcctctg gcggcaaggg
    17881 cgcccagcct actgccgctc cagagtcata acgcaaatca acgtagctaa tccgtttgcc
    17941 atcggtttgc gcctgctgct gtaaaaccgg ataaagttct acaaagcgag ccaaacgttt
    18001 catcgtatcg ccccggccaa gattgagctt aatatcgtta ttcagcgtca actgccagga
    18061 acgccgcgcg gtcatcgccg cttccttcag agtaaatctg tcctttgcca gcatctgccc
    18121 catttcgcga tagccctgca acacttcatt ggcgctgcct tccgggccat acagcattgg
    18181 aagcacctgc ttgctggtgc gatctggcgg cacgctgaag gtgtttcctt ccgcgtctac
    18241 catatgttga tcattccacc gcgcaatcgg cacatattca accagatgaa tcttcaattc
    18301 atcaggccac tgctttctga cgctcacctg cttaatccac ggcaggcgtt gttctatttg
    18361 cgtctggatg atgttgacat cctgggtcat aaaggtaccc ggctcaccca atgccaggat
    18421 cgactgccgg atatcgtcat tacgcgtgta atggcgttca ccggtcaaca ccagctttga
    18481 gagcggcagg cgttgcgcat cttccatcca gcccaacacg acccagccgc tcaccaacac
    18541 tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga cgcgttccat tattgcggcg
    18601 agaagaaacc tcttcttcgc tatttcgcgt gttcagagca gcctgcgaca tattagtccg
    18661 ccagttccag aattcgtact accaactgcg agaagctcat acctgcctga cgtgccgcca
    18721 tcggcaccag gctgtggctg gtcatacccg gtgaggtatt ggcttccagc agataaaact
    18781 gtccatcgct gtccagcata acgtcaatac gtccccatcc tttgcaacct aacgtcgtcc
    18841 atgctttcag cactaatgcc tgcaaattgg cctcttgtga cgcttccaga cctgcggggc
    18901 agaaatactg tgtctcatca gagagatact tcgcctcata atcatagaag gttccggacg
    18961 gttgaatacg tattgacggt aaaatttctt caccgagtat cgcaaccgtg aactccggcc
    19021 cacttagcca tttttcaatc aatacttctt catcgtgctg aaatgccaat cttaatgcat
    19081 cttgtagagc attttctgct actacttttg acattcccac actggaacct tcgcggctcg
    19141 gcttaacgat aaccggcaaa cccagagcag aaatttctgc taactgctta tcgctcaggc
    19201 ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc gaccggtaaa ccggcacctt
    19261 gccacagcag tttgctgcgt agtttatcca ttgaaagcgc agatgccatc actccgctgc
    19321 cggtataagg caagcccatc agctcaagca tgccctgcag cgtaccatct tcaccgccgc
    19381 gaccgtgtag cgcgataaac actttctgaa aacccatcga cttcagttgc gtcacgtcga
    19441 cttctttcgg gtcgacagga tacgcatcaa taccgccttc acgcagcccg gctaacaccg
    19501 ctgcgccaga attcagagaa acttcccgct cagcggaagt cccgcccaac aggaccgcaa
    19561 ttttatcagt catgttgttc ttcctccgga gtttgcggct tcagtttgat ttcagctaaa
    19621 gaacgggcaa tttttccgat attaccagcc ccctgaacga gaatcaggtc gttaccgatt
    19681 aataccggtg ccagcatctc ggctacctgc gcagggtccg gcaccagaat gggatcaatt
    19741 ttcccacgtc cacgaattgt gcgacacagc gaacggctgt ccgctcccgg aattggcgct
    19801 tcgccagccg gatacacttc cagcatcaac agggtatcaa cctgcgtcag cacattggcg
    19861 aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg gctgaaacag cattactagg
    19921 tttttatccg gccagcctgc gcgcgccgct ttaatggtgg cgtcaacttc cgtcgggtgg
    19981 tggccgtagt catcgaccag cattgccgta ccgcttttac cattcactgg ctccagcggg
    20041 aattcaccga ggaaatcaaa acggcgacca gtcccctgga agctctccag cgcacgcaaa
    20101 atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa ccgcagctgc ggcgttcagc
    20161 gcgttatgac ggcctggcgc attcagggtg acgcgcatcg gctctttgtc ctggcgcagc
    20221 agcgtaaagt gcccctgcgg gccaatctgc tgataatctt ctacacgcac gtcggcatct
    20281 tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg gtaacaattc gcggatcacc
    20341 ggatcatcaa cacacatcac cgcacgaccg taaaacggca ggttgtgcag aaaattaata
    20401 aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat ccatgtggtc ggcttcgata
    20461 ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg atgcatcact ctcatctgct
    20521 tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa cccccgccgc ttttaccagc
    20581 ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt agatgctgga aaccatcgcg
    20641 gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc catgacgaaa acgcattaac
    20701 tcagccagca tttcggcacg acggatcacc ggaatacgcg cttcatgagc tgcgacaatt
    20761 tccgggttat cggcagaaat ggcgctggaa acaacgacca cgctggcatc acgtacgttt
    20821 tccgggcgat ggttgaaata aatcgtcgct cccagattca ttaactgctg cgtgaccgga
    20881 tttggcgcta aatcggaacc actgatctga taaccttcat tggccagaac ttcggcaata
    20941 ccgcccatac cggcaccacc gatgccgaca aaatgtatgt gccgaacgcg acgcatttcg
    21001 ggcacgatgg aacgcagttt tgccaattgt tgtgtattca ttctttacgc cattaacttc
    21061 ttaaattcat acgatgcaaa aggcatcgct acaattacgc ccgggcagcc cggctcactt
    21121 catttgccac tcgctcggtg gcatccggaa tggatgcagc gcgggcgcgt tctgccatgg
    21181 ttaataaggt ttctcgcgac cacccggcca gggtgttggc gacagcatcc acgctaagct
    21241 gtggctgctc gataattttg gctgcgcccg ctttttccag cggtagcgca ttccagtatt
    21301 gctggcggtc tttatgttga aacggcacaa acaacgccgg tagtcctgcc gcggcgattt
    21361 cactcaccgt taaagcaccg gagcggcaaa cgacgacatc cgcccacgca tacgccgccg
    21421 ccatatcatc aataaattcc gtcactttat gctgcggttg ccccgcttcg gcatacgcct
    21481 gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca gatagtgact gaatcaccca
    21541 gtttcgcagc aacctgcggc attgtctggt taagaatgcg tgcgccctgg gaaccaccca
    21601 ctaccagcac acgaaccgga ccttcacgtc cagccaaacg ttgctgcggc aacggcagcg
    21661 ccagcacatc ggtacgcacc gggttaccca ccacttccgc attagggaaa gcgcctggaa
    21721 acgcctgcat cactttggtg gcaatcttcg ccagccattt attggttaag cccgcaatac
    21781 cgttttgttc atgaagtaca accggaatgc ctaacgacca cgcggccaga ccacctggac
    21841 ctgatacata gccgcccata ccgagtacca cgtcaggttt gtacgctttc ataatcgccc
    21901 gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc tatcagtgct tttatacctt
    21961 ttccacgcag accagagata cgaatgaaat caatttcgat gccatgtttt ggcactaagt
    22021 ccgcttccat acggtcggca gtccccagcc agcgaacttg ccaaccctga gccattagat
    22081 ggtgcgcaac cgccagtccc gggaatacat gtccaccggt tccgcctgcc atcaccatta
    22141 atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac gcctgcgctt tctccagacg
    22201 cgtttcataa tcaatacgca acagcatcat gatggctgtc gacataatca gtaagctcga
    22261 accaccgtaa ctgatcagcg gcaatgtcag acctttggtc ggtaacatcc ccgccgccgc
    22321 gcctacgtta accagcgcct ggaagctaaa ccagatgcca atagaacagg cgagaaaacc
    22381 ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc gacatcgcgc gaaaagcgac
    22441 gaagaatacc attaaaagtg ccagcaccac accgacatac cccagttctt cgccgataat
    22501 ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc agtttttgta ccgagttacc
    22561 taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc agcgattgcg ttaactgata
    22621 gccgctgcca aagggatctt cccacgggtt ccagaatgcg gtaacacggc ggatacggta
    22681 cggttcggcg agtatcagca acacaaccgc tgaaatgccc ataccgataa tggcaatgaa
    22741 ctgccacaat ttcgctcccg ccaggaacaa catcgccagc gtagtcacaa acaacaccac
    22801 caccgtacca aggtctggct gtgccagcag taacactgcc aacaccagaa tcacgcccat
    22861 cggtttcagg aagccgcgca ggttattacg tacttcgtcg cctttacgca ccagatagtt
    22921 ggcgatataa caaaacagcg acagttttgt cagctccgca ggctggatac gcagcaaacc
    22981 gagatcgatc caacgcgatg cccctttaac cgagctaccc actaccagca cgatcatcag
    23041 caggatgata gatccgagca gcatcgtggc actgtagcgt tgccagaact ccatcggcag
    23101 acgcagcgta atgatcgcca gaatgaatgc caggatcaga tagacgccat cacgcttcgc
    23161 gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc attgacgccg aggtcaccat
    23221 gataaagcca atcgccgcaa ggccgaaggt cagccacagt aaggtgcgat cgtacatgat
    23281 caggctgtcg gtatcttttt cccgcgagcc catcacccag ccttttagcg ccgtggagat
    23341 ccagaccagg atactgaatc ctggcaggcg cggcattttc aggcgaggga gagataaacg
    23401 catcaaccta actccttcgc cagacgggca aactcattgc ctcgttgttc aaagttcttg
    23461 aactgatcaa ggctggcaca ggccggggag agcagaacca tatcgcccag ctgaacacgc
    23521 ggagccagca agcgcatcgc ttgttccatg gtttcggttt gttctgccac ttccgggcgt
    23581 agcgccgcca gctgcgcacc gtcacgacca aaacagtaca aacgcacgtt gtcgccattc
    23641 agatagcgcg ccagtggact gaagtccgcc gatttaccat cgccgcccag caacaaatgc
    23701 agcgtgccgt ctacctgcaa gccattcaac gccgcttccg tactgccgac gttagtggct
    23761 ttcgaatcgt taatccagcg tacgccattg tgctccagca ccacttcaaa gcgatgcggc
    23821 agaccggtga aggtggttaa cgctttcagg ctgctggcgc gcggcaaacc agctgcgtct
    23881 gccagtgcca gtgctgccag cgcattggtg tagttatgct gcccggaaag tttcatctct
    23941 ttcacattca gcactttctc gcctttaacc cgcagccagg tttcgccctg ctgatgattc
    24001 aggtgatagt cacccatgtt gacgccaaag ctgacgcagc gttcatcagc accgcgaatc
    24061 ggcattgtta aggcatcatc agcattgacg acacaaactt tcgcattttc gtaaatacgc
    24121 aattttgccg cacggtattg ctgcaaacca aacggatagc ggtccatatg atcttcagtc
    24181 acgttcagaa tggtcgccgc caccgcctgt aagctggaag tggtttccag ctggaagctc
    24241 gacagttcca gcacgtacag ttcgcactca gcatcaagca gcatcaacgc tggcaggcca
    24301 atattgccac ccacaccaac gttaaccccc gccgctttcg ccatttcacc cactagcgtg
    24361 gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa tcggtgcttg tgcttcgcga
    24421 cagaacagct cgatatcgcc aacgatttcg attccggcat cagcggcagc gcttaaggat
    24481 ggatgcgcct gtgcaatacc gggactggcg acaatcagat ctgccgccat cagccattca
    24541 tcattcagac tgcccgtgtg gcgttctacg gcttcgggta atttatccag gccaggcggt
    24601 gtcatacgcg tatccataac gcgcggcgtc acaccgcgag cgaggaaaaa gtccacgcag
    24661 gaaagcccgg tgaggcccag gccgataatg acgacatttt taccctgata atcagccatg
    24721 attaacgtac cttcagcgtt gccagaccaa tcagaaccag catcagcgaa ataatccaga
    24781 aacgcacaat gacgcgcggt tccggccagc ctttcagttc atagtggtga tgaatcggtg
    24841 ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc gacctgcagg atgacagaaa
    24901 gcgtttctac cacgaacacg ccccccataa tcaccagcag gaattcctga cgtagcagta
    24961 cggcgataat gcctaacgca ccacctaacg ccagcgaacc tacatcgccc ataaagacct
    25021 gcgccggata ggtgttaaac cacaggaagc ccagtcctgc cccgactatc gccgtgcaga
    25081 caatgaccag ttcccctgcg tgtcgcagat acggtatatg caagtagctg gcaaagttca
    25141 tattgccggt cgcccacgcc accagcgcaa aaccaccggc gacaaatacg gtcggcataa
    25201 ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt gccagtaccc acaatgacga
    25261 agtaagccag cagaatgtag aacagcccca gctgcggcat cacatcttta aagaatggga
    25321 ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta cagggcgaag gcgacaccca
    25381 gcgcaatgac cgacatccag aaatacttcc aacgagcgat caaccctttg gtgtctttac
    25441 gcaccacttt gcgataatca tcaacaaagc caataacacc gtaacctacc agcaccacca
    25501 acacgcacca gacgtacgga ttggacgggt aagcccacag cagtacggag atcacaatcg
    25561 ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg cttgctgaag tgtgattcag
    25621 gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg caaatgagct atcatacgcg
    25681 ggcccatcca caatgagatg aacagcgcgg tcagcaggct gacgatggcg cgaaacgtca
    25741 gataggaaaa gacgttaaag ccggaataat atttgaccaa atgttcggcc agccaaacta
    25801 acatgtccca ttctcctgta aagcgcgtac tacctcttcc atggcggcac tacgtgaacc
    25861 cttaactaaa atcgtaatta cctgttgctc agcaatcagt gatttaagac gcgtaattaa
    25921 cgcagtttta tcagcaaaat gttcgccaac gccgctggcg gtgctgatag catggctttg
    25981 tttacccacg cttaacacgc ggtcaatacc agcagctttt gccgcctcgc ccacctgtac
    26041 atggcaggct tcgctttcag cgcccagttc cgccatatcg cccaccacca gcacgcggta
    26101 gcccggcatt tcagccagta cctgaacagc ggcggtcatt gaaccgacat tggcgttgta
    26161 ggagtcgtcg agcagcaact ggttttctgc cagttggatg gggaacagac ggcctggaac
    26221 agctttcaga tttgccagcc ccgctttgat agcatcaagc gttgcgccca cggacatgga
    26281 gagcgcagcg gctgccagcg cattcgcaat attgtgacgc cccggcaacg gcagcagaac
    26341 atcgacgcta ccggttgggg tttgtagggt aaattccgta ccgtgcgagg tcacatggat
    26401 attggtggcg gtgaaatcgc tgttggcggc attgggtgag aaacgccaca ctttgcgtga
    26461 gccaattacg ctctgccagt tcagccagtc gttgttgtcg gcgttcataa tggcgatacc
    26521 gttttccggc aggccgctaa agatttcacc tttcgctttc gcgacacccg caagcgagcc
    26581 aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc gcagcttccg ggcgagtcag
    26641 actcacagtc caggctattt cgccctgatg gttcgcgcca agttcaataa ctgcgtaatc
    26701 gtattccggc gtcaagcgca acaacgtcat cggtacaccg atgtcgttgt tgagattgcc
    26761 tgccgtataa agcgtgttgc cgcactggct taaaatcgcc gccgtcatct ctttaacgga
    26821 ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc ggaacttgct ggcgaaccca
    26881 tgcagccagt tcaccaaacg ccagacgcgt atccttgacg attaactgcg gcaggtcgat
    26941 atccagcgga cggctgacca gtagtgcgcc cgcgccgcca gctttcgcct ggtcggcaaa
    27001 atcatgagca tcgaaacgtt cgcctttcag ggcaacaaac aggcagcccg gcgtcagttt
    27061 tcgggtatca gtggttacag catcaagggt gatatctgca ccttgcagtt caccgttgag
    27121 aatgtcggta agttggctaa gggttacgct aatcatgcaa tcacccccag cagacgcgcc
    27181 accgtgacgc gatcggagta gtccagacgc tgattgccaa caatctggta atcttcatgg
    27241 cctttgcccg cgaccagtac cacatcattc tctttagcct gcataacggc gcaagtcacc
    27301 gcttcagcac ggccttccat cactttggca tgtccggcat ctaacattcc cgccagaata
    27361 tcgttgatga tggcacgcgg ttcttcggta cgcgggttat cgtccgtcac caccgccacg
    27421 tcagcaaact cttcggcaat tgcgcccatc agtggacgct tacctttatc gcgatcgcca
    27481 ccacagccaa agacgcacca cagcttgccc gcacagtgca ggcgcgccgc ctgtaaggct
    27541 ttttccagtg catccggcgt atgcgcgtaa tccaccacca ccgtcggttt gcctggcgca
    27601 gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg cggcggtttt cagcagatca
    27661 gccagtggat agccgagtgc cagcagtgtc gccagcgcga gcagcaggtt gctgacgtta
    27721 aaagcgccca tcagatggct ttcaatttcg ccatcgcccc aacttgagct aaagcgaatc
    27781 gtcgcaccgc tgtcgtgata gttcacgtcg atcgctttta accagcgtcc gtgacagttc
    27841 ggattaatat gatcttccat tgataccgca accgcgtccg gcagttttgc cagccagcgg
    27901 cggcccactt catcgtcggc gttaataatc gcctgaccgc aatgatgttc agaataaagc
    27961 agccatttcg cagcttcgta gtgttccata tcaccatgat aatcaaggtg atcgcggctt
    28021 aagttggtaa agaccgacgc cgcaaatttc aatgccgcca cacggtgctg taccagcccg
    28081 tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct ccaccagccc cgccagctca
    28141 tgctgaacat cgactgcgga accggtagta ttttctgtcg ggatcacttt cccaagcaga
    28201 ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa gcagttggct ccactgcgcc
    28261 agcagctggg tagtcgtggt tttgccgttg gtgcccgtta cgcctacgag acgtaaattg
    28321 tcagagggtt catggtaaaa gcggcccgcc agtgcagata aacgctcatt gagctggctg
    28381 agatagatga ccggtacgcc gtgcatttca cggatttcac cgtcggtcgc ctcatctttc
    28441 gctttcgcct ctgcaataat ggcagccaca ccttgcgcta tcgcctgcgg gatatatcga
    28501 cgcccgtccg cctgatgacc tactacagct acaaagagat cgcccgccgc agccacacgg
    28561 ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt ctggcaccca cggagcaaga
    28621 aggtcgcgca aattacgatc tgccacctgt cccctcgcct tgattaatca caaattcatt
    28681 tttatcgccc gttgtcagcg catccggctc gatgttcatg gtacgcaata cgccgcccat
    28741 gatggcacca aagaccggcg cggaaacggc gccgccgtag tatttacccg cctgcggatc
    28801 gttgataaca acaaccagcg cgaagcgcgg ctgactcgca ggcgcaacgc ctgcggtata
    28861 agcaatatat ttattgatgt agcgaccgtc cggcccgacc tttttcgcgg taccggtttt
    28921 aatggcgata cgatagcctt taatcgccgc cttcacgccg ccgccgcctg gtagcgccac
    28981 gctttccatc atatgcacca cagtgcggac aatggatccc gggaagacac gttcaccggg
    29041 aaccgggggg tcaactttgg taatcgacag tgggcgataa atgccgtagc tgccgatagt
    29101 tgcgtagact cgcgctaact gtaacggtgt taccattagc ccgtagccga aagagaaggt
    29161 ggccctctct atgtcagacc accgttgttt ttgaggatat aagccactgc gttctccgac
    29221 caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag taagtatcta ctaacgctga
    29281 ggacggcatc gctaacgcca gcttggaaac accgacgtta ctcgacttct gtaatacccc
    29341 ggtcagggtt aattcgctgt agcgtgccac gtctttgatt tcgtggccgt taattcgata
    29401 aggaatggta ttgagtaccg agttttcccg caccacgcca cgttgcaacg cggtcattac
    29461 caccatcggt ttaaccgttg agcccggttc aaacacgtcg gtgatggtac ggttacgcat
    29521 cgcctctttc ggcgtgccgc tcagattgtt agggttgtat gacgggctgt tagccatcgc
    29581 cagcacttca ccggtgttga catccaccag cacggcgcta ccagattcag ccttgttaaa
    29641 ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc aggcgttcat caatactcag
    29701 cgccaggttg tgcgctgcct ggctgtcagt agaagaaata tcttcaatta cgcgaccata
    29761 gcggtcttta cgcacaatgc gctcacccgg ctgcccggta agccatttat cgaaactctt
    29821 ctcaacgccc tcaatccctt gactatcgac gttagtaaag ccgatgaggt gagcagtcac
    29881 ttcgccggac ggatagtaac ggcgagactc ttcacgcaga tgaatccccg gcagtttcag
    29941 ttttttgatg tagtccgcca tgtcagggtt cacctgacgc gccagataaa taaaacgccc
    30001 tttcgggttg gcgttaatgc gggctgaaag ctgatccagc ggaatattga gcgcgttagc
    30061 cagcgccttc cagcggtcac cgacgctgat accgccagcg tcatgcactt ctttcgggtc
    30121 agcccaaatc gcttttaccg gcacgctcac cgctaacggg cgaccagaac ggtcagtaat
    30181 catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa cgcatgtcgc cctctttcac
    30241 cagcatatcc ggggagataa cttgtaacca cgctacgcgt ccgagcagaa aagccagcgc
    30301 caggagaata cagccgcata acaacgcaaa acgccaactg ataaagttgg catgttcttc
    30361 ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg cgtcgcgttt atccttattt
    30421 ttgcactacg atattttctt gtgacggatc aacatgctgc atttgcagct tttccgtggc
    30481 gatcctttcc acccggctat ggtcgccgag cgcattctct tcaaggatca ggttgcgcca
    30541 ttcaatgtct aaagcatctc gctccagcac cagttgttcg cgctgagcgg tcagtaaacg
    30601 ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa ataatgcaaa tgaacaggca
    30661 gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata acaccaggca atgcatggcg
    30721 ctcgtggctt cccatcgatc ctttaacttt gcttagagct tctgtcactc tgctgatcat
    30781 gcattcgtcc tctctgcaat acgcagaact gaactacggg cacgagggtt ctcagccacc
    30841 tcttcttcgc ccggcattaa cttgcctagt gctcgcagct gacggccacc cagttttttg
    30901 agctgctctt cagtcatcgg taaccctgcc ggaacttgcg gaccgcggct gttttcacgc
    30961 ataaaacgtt tcacaatacg gtcttccagc gagtggaagc tgatgatcga aagccgccca
    31021 cccggggcca gcacgttgag cgagcttttt agcgcctgct ctatctcctc cagttcactg
    31081 tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg gatgtttaaa cttatctttc
    31141 accggcgttg caacagccac gacttccgcc agttctttgg tgcgggtcat cggctgttcg
    31201 cggttacgct cgacaatggc gcgggcaatg cgtttggcaa aacgctcttc accataggtt
    31261 ttcaataccc aagcgatatc ggcttcttct gcggtttgta gccattcagc ggctgactgc
    31321 ccacgggttg ggtccatacg catgtccagc ggaccatcgc gcataaagga aaagccgcgc
    31381 tcagcatcat caagttgcgg tgaagagacg ccaagatcga ggagaatgcc gtcgatcttg
    31441 ccgataagat cgcgctcggc aacgtattcg cccagcgcgg agaaaggtcc gtggatgatg
    31501 gagaagcgcg gatcatcaat agtcttcgca acggcgatag cctgcgggtc gcgatcgatc
    31561 gccagcaaac gcccctcttc gccaagctgc gagaggatca gacgtgagtg accaccgcga
    31621 ccaaaagtcc catcaatgta gatgccatca ggacggatat tgaggccatt aacggcttca
    31681 tccagcagca ccgtagtatg tttatagttt tccatcattt tatagagaca agtcctgcag
    31741 tcgctccgat aagtctccgg tagccaactg ctctgcgtcg atatcttcct tgacctgttg
    31801 atgccaggtt gtttcatccc acagctcaaa cttgttgaac tgtccaacca gcatcacttc
    31861 ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt aacaatcgac ctgcgccatc
    31921 catctgacat tcgctggcat gacctaacag taggcgctgc acacggcgct caaccgggtt
    31981 catgctcgac agacgcgata atttttgctc gataatttcc cattcaggca gggggtaaag
    32041 cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt tgaccgacag cgttctcaag
    32101 cagctgttcc cggtaacggg taggcactga taagcgccct ttgctgtcga gattgactaa
    32161 cgttgctccc cggaacatgc cagcctcacc ccttattccc actttgcccc acaatttccc
    32221 actgaaagga gtttacggag cggaggaaaa gcttgtcaag ccagtcacaa ggcataccga
    32281 ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt tattttacgt ccaacgtgcg
    32341 cagcaaatta acgttatgaa tagctgtaag aaaaaatccc tcatcgtcat caccgtttta
    32401 aaatgactca ctattttcca cagcaaatat aaagtgtcag tttgcgacgc gagcggcatt
    32461 ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc tgtgtggcgc ttgatgccag
    32521 cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg gcaattcatc tctgcatgta
    32581 acaaaataat gcaaaaacgc gctgtcgata tcacttaatt attgtgaatt tcagctggtg
    32641 agttaattaa cgggcgggtt taaaagaact tatcaataat agtcaggaat agtcttattt
    32701 actttaagga tattgatgtc cattcccgta ctctacg
//
LOCUS       DAZOYL010000011        32233 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZOYL010000011 DAZOYL010000000
VERSION     DAZOYL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.011"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..555
                     /locus_tag="ACHUOY_000377"
     CDS             <1..555
                     /locus_tag="ACHUOY_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0681267.1"
                     /translation="FVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIVP
                     DDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGN
                     YYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKAW
                     LVGAADAPDLGNFEANTTFQITYL"
     gene            637..3279
                     /gene="yfcU"
                     /locus_tag="ACHUOY_000378"
     CDS             637..3279
                     /gene="yfcU"
                     /locus_tag="ACHUOY_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFR0681268.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3299..4050
                     /locus_tag="ACHUOY_000379"
                     /pseudo
     CDS             3299..4050
                     /locus_tag="ACHUOY_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            4025..4573
                     /locus_tag="ACHUOY_000380"
     CDS             4025..4573
                     /locus_tag="ACHUOY_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0681269.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4570..5040
                     /locus_tag="ACHUOY_000381"
     CDS             4570..5040
                     /locus_tag="ACHUOY_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0681270.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5037..5561
                     /locus_tag="ACHUOY_000382"
     CDS             5037..5561
                     /locus_tag="ACHUOY_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0681271.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5548..6420
                     /locus_tag="ACHUOY_000383"
     CDS             5548..6420
                     /locus_tag="ACHUOY_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR0681272.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6542..7093)
                     /gene="smrB"
                     /locus_tag="ACHUOY_000384"
     CDS             complement(6542..7093)
                     /gene="smrB"
                     /locus_tag="ACHUOY_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR0681273.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7259..8191
                     /gene="prmB"
                     /locus_tag="ACHUOY_000385"
     CDS             7259..8191
                     /gene="prmB"
                     /locus_tag="ACHUOY_000385"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0681274.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            8226..9311
                     /gene="aroC"
                     /locus_tag="ACHUOY_000386"
     CDS             8226..9311
                     /gene="aroC"
                     /locus_tag="ACHUOY_000386"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR0681275.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9315..10139
                     /gene="mepA"
                     /locus_tag="ACHUOY_000387"
     CDS             9315..10139
                     /gene="mepA"
                     /locus_tag="ACHUOY_000387"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR0681276.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10139..10948
                     /locus_tag="ACHUOY_000388"
     CDS             10139..10948
                     /locus_tag="ACHUOY_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR0681277.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            10948..11496
                     /gene="epmC"
                     /locus_tag="ACHUOY_000389"
     CDS             10948..11496
                     /gene="epmC"
                     /locus_tag="ACHUOY_000389"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR0681278.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11530..11808
                     /locus_tag="ACHUOY_000390"
     CDS             11530..11808
                     /locus_tag="ACHUOY_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR0681279.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(11929..13935)
                     /gene="mnmC"
                     /locus_tag="ACHUOY_000391"
     CDS             complement(11929..13935)
                     /gene="mnmC"
                     /locus_tag="ACHUOY_000391"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR0681280.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            14094..15314
                     /gene="fabB"
                     /locus_tag="ACHUOY_000392"
     CDS             14094..15314
                     /gene="fabB"
                     /locus_tag="ACHUOY_000392"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR0681281.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15589..16767
                     /locus_tag="ACHUOY_000393"
     CDS             15589..16767
                     /locus_tag="ACHUOY_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0681282.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16764..17759)
                     /gene="flk"
                     /locus_tag="ACHUOY_000394"
     CDS             complement(16764..17759)
                     /gene="flk"
                     /locus_tag="ACHUOY_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR0681283.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            17858..18994
                     /gene="pdxB"
                     /locus_tag="ACHUOY_000395"
     CDS             17858..18994
                     /gene="pdxB"
                     /locus_tag="ACHUOY_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR0681284.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            19060..20073
                     /locus_tag="ACHUOY_000396"
     CDS             19060..20073
                     /locus_tag="ACHUOY_000396"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0681285.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            20073..20885
                     /gene="truA"
                     /locus_tag="ACHUOY_000397"
     CDS             20073..20885
                     /gene="truA"
                     /locus_tag="ACHUOY_000397"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR0681286.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            20968..21627
                     /locus_tag="ACHUOY_000398"
     CDS             20968..21627
                     /locus_tag="ACHUOY_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0681287.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            21783..22697
                     /gene="accD"
                     /locus_tag="ACHUOY_000399"
     CDS             21783..22697
                     /gene="accD"
                     /locus_tag="ACHUOY_000399"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR0681288.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            22767..24035
                     /gene="folC"
                     /locus_tag="ACHUOY_000400"
     CDS             22767..24035
                     /gene="folC"
                     /locus_tag="ACHUOY_000400"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR0681289.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            24025..24687
                     /gene="dedD"
                     /locus_tag="ACHUOY_000401"
     CDS             24025..24687
                     /gene="dedD"
                     /locus_tag="ACHUOY_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR0681290.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            24946..25434
                     /gene="cvpA"
                     /locus_tag="ACHUOY_000402"
     CDS             24946..25434
                     /gene="cvpA"
                     /locus_tag="ACHUOY_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR0681291.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            25471..26988
                     /gene="purF"
                     /locus_tag="ACHUOY_000403"
     CDS             25471..26988
                     /gene="purF"
                     /locus_tag="ACHUOY_000403"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR0681292.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            27083..27652
                     /gene="ubiX"
                     /locus_tag="ACHUOY_000404"
     CDS             27083..27652
                     /gene="ubiX"
                     /locus_tag="ACHUOY_000404"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR0681293.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            27918..28700
                     /gene="argT"
                     /locus_tag="ACHUOY_000405"
     CDS             27918..28700
                     /gene="argT"
                     /locus_tag="ACHUOY_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR0681294.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            28921..29703
                     /gene="hisJ"
                     /locus_tag="ACHUOY_000406"
     CDS             28921..29703
                     /gene="hisJ"
                     /locus_tag="ACHUOY_000406"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR0681295.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            29793..30479
                     /gene="hisQ"
                     /locus_tag="ACHUOY_000407"
     CDS             29793..30479
                     /gene="hisQ"
                     /locus_tag="ACHUOY_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR0681296.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            30476..31192
                     /locus_tag="ACHUOY_000408"
     CDS             30476..31192
                     /locus_tag="ACHUOY_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0681297.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            31200..31973
                     /gene="hisP"
                     /locus_tag="ACHUOY_000409"
     CDS             31200..31973
                     /gene="hisP"
                     /locus_tag="ACHUOY_000409"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR0681298.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            32170..>32233
                     /locus_tag="ACHUOY_000410"
     CDS             32170..>32233
                     /locus_tag="ACHUOY_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681299.1"
                     /translation="MTISTTSTPHDAVFKSFLRHP"
BASE COUNT         7548 a         7991 c         8722 g         7972 t
ORIGIN      
        1 tttgttaaaa cagctattgc tgcggcaatg gtgatgggcg tgttcacctc tacggcaaca
       61 atcgctgcgg gtaacaacgg tactgcacgt ttttacggca ccattgaaga ctcagtgtgt
      121 tctatcgttc cggacgatca caaactggaa gttgatatgg gtgatatcgg tgccgaaaaa
      181 ctgaaaaata acggcaccac cacgccgaaa agtttccaga ttcgtctgca agattgcgta
      241 tttgatactc aggaaacaat gaccactacc tttactggta ccgtttcttc tgcaaatagc
      301 ggcaattatt acaccatttt caataccgat acctgtgcgg catttaacaa tgttagcctg
      361 gcgatcggtg actctctggg tacctcttac aaaagcggca tgggtattga ccagaaaata
      421 gtgaaagaca cttctaccaa taaaggcaaa gcgaagcaga cactgaactt taaagcctgg
      481 ctggtcggcg cagctgatgc gccagatctg ggtaatttcg aagcaaatac caccttccag
      541 attacttacc tgtaaggcat acccccatag cgacactgct atgggggatt taaatggatt
      601 ttgaccgaac aagaataaaa acggatagaa acgtgtatgc ctgaccattc tctttttcga
      661 ttacggattc ttccgtggtg cattgcgctg gcaatgtcag ggagttatag cagtgtctgg
      721 gctgaagacg acattcagtt tgattcccgt tttctggaat taaaaggcga cacaaaaatt
      781 gatctgaagc gtttttccag ccagggatat gttgagcccg gaaaatacaa tttacaggtt
      841 caactaaata aacagccatt ggcggaagag tacgatattt actggtatgc tggtgaagat
      901 gacgcgagca aaagctatgc ttgtctgaca ccggaactgg tagcgcagtt tggtttaaaa
      961 gaagacgtgg cgaaaaatct gcaatggagc cacgatgcta aatgcctgaa atccggtcaa
     1021 ctggaaggca tggaaattaa ggctgattta agccagtccg cattagtcat ttcactgcca
     1081 caggcttacc tcgaatatac ttatcccgac tgggatccgc cttcacgttg ggatgacggc
     1141 atctccggga tcgtcgcgga ctacagcatc aacgcacaaa cccggcacga agaaaatggc
     1201 ggtgatgata gtaacgagat cagcggcaac gggacggtcg gggttaacct ggggccgtgg
     1261 cgtatgcgtg ctgactggca gactaactat caacatactc gcagtaatga tgacgatgaa
     1321 ttcagcggcg atgaaactca aaaaaaatgg gagtggagtc gctactatgc ctggcgggcg
     1381 ttaccatcat taaaagccaa actggcgctg ggcgaggatt acctcagatc cgatattttt
     1441 gatggtttta actatgttgg tggcagtgtc agtactgacg atcaaatgtt gcctcccaat
     1501 ctgcgcggct acgcgccaga catttccggc gtggcacaca ccacagcaaa agtgaccgtc
     1561 agccagatgg ggcgtgtgat ttacgaaacg caggttccgg ctggaccgtt tcgtattcag
     1621 gatcttggtg attctgtctc cggtacgttg catattcgca ttgaagaaca gaacggccag
     1681 gtgcaggaat atgacatcag caccgcctcg atgccatacc tcactcgtcc aggtcaggtt
     1741 cgctataaga tcatgatggg ccgtccgcaa gagtggggac accatgtcga gggtgaattt
     1801 ttttctggtg ctgaagcttc ctgggggatc gctaacggct ggtcgttata tggcggcgca
     1861 ctgggagatg aaaactatca gtctgcggcg cttggcgtcg gtcgcgattt gtctacattc
     1921 ggcgcggtcg cgtttgatgt tactcactcg cacaccaaac tggataaaga caccgcttat
     1981 ggcaaaggtt cgctggacgg taactccttc cgtgtgagtt attccaaaga ctttgaccag
     2041 ctcaacagcc gcgttacttt tgctggatat cgcttctcgg aagagaactt tatgaccatg
     2101 agcgagtatc tggatgccag tgacagcgga atggtacgca cgggcaacga caaagagatg
     2161 tacactgcca cttataacca gaacttccgc gatgcgggtg tttcggttta tctcaactat
     2221 acccgccata cctactggga tcgcgaggag cagacaaact acaacatcat gctctcgcac
     2281 tatttcaata taggcagtat tcgtaatgtc agcatctcga tgactggcta ccgttacgag
     2341 tatgacaacc aggccgacaa aggcatgtac atttcgctca gtatgccgtg gggtgacaac
     2401 agtaccgtta gctataacgg taactatggc agtgggacgg acagcagtca ggtcggttat
     2461 ttcagccgtg tcgatgacgc gactcactat cagttgaacg tcggcaccag tgacaaacac
     2521 accagcgttg acggctatta cagccatgat ggttcgctgg cgcaggttga cctcagtgcg
     2581 aactaccatg aagggcaata cacctctgcg ggcttgtcgt tacagggcgg cgcaacgctt
     2641 actgcccacg gtggcgcact tcaccgtacc cagaatatgg gcgggacacg cttgttgatt
     2701 gatgccgatg gcgttgccga tgttccggta gaaggtaacg gtgctgctgt ttataccaat
     2761 atgtttggta aagccgtcgt ttctgacgtc aataactatt accgcaatca ggcgtatatc
     2821 gacctcaaca aactgccgga aaacgccgaa gcaacccagt cggtggtaca agccacgcta
     2881 actgaagggg cgattggcta ccgcaaattt gccgtcatca gtggtcaaaa agcgatggcg
     2941 gtgctgcgtt tacaagacgg cagccatcct ccgtttggcg cagaagtaaa aaatgataac
     3001 gagcagacag tgggccttgt cgatgatgac ggcaatgttt atctggctgg ggtgaaacct
     3061 ggcgaacaca tgagtgtgtt ctggagtggt gttgcgcatt gcgatatcaa cctgccggac
     3121 ccgctgcctg ccgatctgtt taacggcttg ttactgccat gccagcataa aggcaacgtc
     3181 gcgccggttg ttcctgatga cataaagcct gtcattcagg agcagacgca acaggtgaca
     3241 cccaccaatc cccctgtttc tgtttcagct aaccaataac gtgattaagg aatgatccat
     3301 gtctgattta ctttgctctg ccaaattggg tgctacgacg ctagcgttac tgcttagcgc
     3361 tgccagtttg tcgcccaggc ttctgtaacg ccagatcgta cccgtttgat atttaatgaa
     3421 agcgacaaat caatcagcgt gacgttgcgc aataacgatc cgaaactgcc ttatctggcc
     3481 caaagctgga tcgaagatga aaagggtaac aagatcagtt caccgttaac tgttctgcca
     3541 ccggtgcaac gcatcgattc aatgatgaac gggcaggtaa aagtgcaggg aatgccggat
     3601 atcaacaaat tgcctgccga tcgtgaaagc ctgttctact tcaacgtgcg tgagatcccg
     3661 ccaaaatcaa acaaagccaa cacgctgcaa atagcactac aaacgcgtat taagttgttc
     3721 tggcgaccga aggcgctgga gaacgtcagc atgaaaaacc cctggcaata taaggttact
     3781 ttaacccgca acggtcagga atttaccgtc aataacccga cgccgtatta cgtcattttc
     3841 agtaatgcca gcacacagaa aaatggcaat cctgcagccg gtttctcgcc aatagtgatg
     3901 tcgccgaaaa cctctgcacc gctgaatgtg aagatgggta gcgtgccggt actgacctat
     3961 gtcaacgact atggcgctcg gatgccgctc tttttcactt gcaacggtaa cagttgccag
     4021 gtcgatgaag agcagagccg caaaggctga ggccgggaga gccagtatga aaggatcttg
     4081 ttctgtctgg gcggtaactg ggttactgat atgttgtgtt ggtagcggct ttgcagttgc
     4141 agcgagtaag tcagtaagta tgaatttacg ggtgcttgtc gatgcccctc ctccttgtac
     4201 ggtaaatggt gcggcagttg agtttggtaa tgtattcatt aataagatca atggggttga
     4261 ttataaacgt cccattgact actcgcttgt ttgtaacaat ctggcaatgg atgacctgcg
     4321 tctacagatg caggcgacaa cggtcgtgat taacggtgaa acagttatca gcacgggtat
     4381 tcctgggttt ggtattcggg ttcagaaaag tagtgatcac acaatactcg acttaacctc
     4441 tggctcatgg ttgccattta atttttcctc gggagttccc gttctggaag ctgtgccggt
     4501 gaaacagagt ggtacaacgt tagcggcagc tgaatttaac gccagtgcca caattgtggt
     4561 ggattaccaa tgaaacggat ctcgcttatt ttgctgtggg gcttttgctc gatggcactc
     4621 tcaaacgtct cttttcacgg ttatttagtg cagccgccta actgcacgat tagcaacgct
     4681 caaaccattg aaataacttt tcaggatgtg cttattgatg acattaatgg cagcaattat
     4741 gagcaaaccg tgccgtattc cattacctgc gatacggcgg ttcgcgaccc attaatggaa
     4801 atgaccttga gctggagtgg cacactaagc gattttgata atgctgcagt aagcagcaat
     4861 atcactggtt taggcattca gctcaaacag gcagggcaga gttttacgat caatacgcca
     4921 cttatggtga atgaaaccga tttaccggtg ttaacggctg tgccagtaaa aaagagtggt
     4981 gtcgttctgc cagaggcaga ctttgaggcc tgggccacgt tacaggtaga ttaccaatga
     5041 agaaaaagcg tacgttgttt tttattagta gcctgatgtt gctgggcagt ggaaccacaa
     5101 tagcaggaga taatttgcac tttaccggca atttaatcag taaatcgtgt acgccagtga
     5161 ttaatggtag ccagttggca gaagtacatt ttcctgccat tgccgccagc gatttaatga
     5221 accttgggca gtcagagcga gtgccattgg tgtttcaatt gaaagattgt catagttcta
     5281 cattgtttaa tgtcaaagta acgttgacag gaacagaaga tagtgctcta cccggctttc
     5341 tggcctttga ctcctcgtca tcagcatccg gcgctgggat tggtattgaa actgcggcag
     5401 gcacatcggt gccaatcaac aatacaacag gcgtcacgct cccgctgaat cagggaaata
     5461 acagcctaaa ttttaatacc tggttacagg caaagagcgg gcgagatgta acgtccggag
     5521 actttagtgc cacagtgacg gcaacctttg agtatttctg aaatgaaaaa atggaccata
     5581 attttcgcga gcctaatgct gctcgttctg agtgttgtcg gcgcatctaa gagttatgct
     5641 ggagataaac ttatgagtgc gagttttgac agtgtgagag tttattatgc catggacaag
     5701 gtaaccggtg ctgtagcgag cagtgttttc aatgttacgg tgataacgcc aaaggaggtt
     5761 acatatggga aatatgattc ttttgcgtat aaaggcgata cgttgcgggt tatttcctgg
     5821 tctgggtcgg gttcagctcc aactttggta ctgactgact ttgatacaat taataactca
     5881 aattgccctg gcatagatac gaaaattttt cgttgtgcat atatgacgtt taaaattacc
     5941 gttgcatctg atgattatgg ttgcccttgg atcgcatcct tttattccta tacagattta
     6001 cccggatttg gaagctatac ggcccctaca gtgcataaca caatatgccc aacgataccg
     6061 gtcacttcat acgatatctc ctggagtgaa aattatgtca gccataataa ggcgttacga
     6121 attcagtcca cggggtcaac agtgaccacc acgctttcga cctacctgat ggaaggcggc
     6181 agactctgtg atggcagtaa tttttctgat aatgatggca gaggagcata ctgccgcgcg
     6241 gtatcagaat tgttaacctt tacctcttac ggttgtgata aatcaacggt aacggttacg
     6301 ccaactcgtc atccggtaac ggataaagta ctacacgata ttgtggtgaa tgtgaatacc
     6361 agtagtgggc aaccgattga ttcgacgtgt cgttttcagt atgtattgaa tgaactgtag
     6421 ttcgtagtat tgctctgatg cgtcttatca ggcctacgaa gtgaattgca atttattgaa
     6481 ttatcacata tttgtaggcc ggataaggcg tttacgctgc tccggcattt gctgccagca
     6541 ttcagggcaa ctccggcggc aaccactctt ccacttcaat caacaccaac aaagccgcat
     6601 caccgccgta ctctttcggt gcctggtgaa atgccatcac atgcggatgt tgtgccagcc
     6661 acagcggtgt ttgttgcttc aaaatatgct tcccgtgccc atgcatcacg caggcacaaa
     6721 acacatgttc gcggcgacag gcggcaatca acgcgcccag ttcctgcttg gcctgcagct
     6781 gcgtcagacc gtgtaaatcc aaaaacaatt ccggtgaata atcgccgcgg cgcagtttct
     6841 tcgcctcaaa atggctgaca tccgggcgaa catatttcac cggaccttcg gtatttaata
     6901 acggctgaaa ctcatcggag aaataatggc tggcatcagc ctgctccggg atcaagcgtt
     6961 tcaccggcac ttcgctgatt tttttacgct gcggtcggtg gacaatcgtg tcctgcttaa
     7021 tcttgcgagt ccccgccatc aactggcgaa acagagcctg gtcctcctcg ctaagtgttg
     7081 ttttcttttt catttatctg tctctatttc taattttcct acagtttacc cgactcgtag
     7141 cacaatcgat cgttttaaac gtatttttcc tcactcaccg cgcgtgaatg ctgatttatc
     7201 gtcatcttca tggcaaacta gccgccgaaa ttattgcgat catgccctgg aggaatacgt
     7261 ggataaaatt ttcgttgatg aagcagtaaa tgagctgcaa accattcagg acatgttgcg
     7321 ctggtcggta agccgcttca gcgcggcaaa tatctggtac ggtcacggca ccgataaccc
     7381 gtgggatgaa gccgtacagc tggtgttacc atcgctctac ctgccgctgg atatcccgga
     7441 agatatgcgc accgcgcgtc tgacctccag cgaaaaacac cgtattgttg aacgcgtgat
     7501 ccgccgcgtc aacgagcgca ttccggtggc ttacctgacc aacaaagcct ggttctgcgg
     7561 tcatgaattt tacgtcgatg aacgcgtgct ggtgccgcgc tcgccgattg gtgaactgat
     7621 caacaataaa tttgccggac tcattagcaa gcaaccgcag catattttag atatgtgtac
     7681 cggtagcggc tgcatcgcca ttgcctgtgc ttatgccttc ccggaagcgg aagtcgacgc
     7741 ggtagatatc tctcccgacg cgctggcggt tgccgaacag aacatcgaag aacacggtct
     7801 gatccacaac gtcattccga ttcgttccga tctgttccgt gatttgccga aagtgcagta
     7861 cgatctgatt gtcactaacc cgccgtatgt cgacgcggaa gatatgtccg acctgccaaa
     7921 cgaataccgt cacgagccgg aactgggcct ggcatctggc accgacggcc ttaaactgac
     7981 gcgtcgcatt cttggaaacg cggcagatta ccttgctgat gatggcgtgt tgatttgtga
     8041 agtcggcaac agcatggtac atcttatgga acaatatccg gatgttccgt tcacctggct
     8101 ggagtttgat aacggcggcg atggtgtgtt tatgctcacc aaagagcagc tgctggcggc
     8161 ccgtgaatat ttcgcgattt ataaagatta agtaaacacg caaacacaac aataacggag
     8221 ccgtgatggc tggaaacaca attggacaac tctttcgcgt aaccaccttc ggcgaatcgc
     8281 atgggctggc gctcggctgc atcgtcgatg gtgttccgcc aggcattccg ctgacggaag
     8341 cggacctgca acatgacctc gaccgtcgtc gccctgggac atcgcgctat accacccagc
     8401 gccgcgagcc ggatcaggtc aaaattctct ccggtgtttt tgaaggcgtt actactggca
     8461 ccagcattgg cttgttgatc gaaaacactg accagcgctc tcaggattac agcgcaatta
     8521 aagacgtttt ccgcccaggc catgctgatt acacctacga acaaaaatac ggtctgcgcg
     8581 attatcgcgg cggtggacgt tcttccgccc gcgaaaccgc catgcgcgtg gcggcagggg
     8641 cgattgccaa aaaatatctc gccgagaaat ttggtattga aatccgcggc tgcttgaccc
     8701 agatgggcga cattccgctg gaaatcaaag actggtcgct ggttgaacaa aatccgttct
     8761 tctgcccgga cccggacaaa atcgacgcgt tagatgaact gatgcgcgcg ctgaaaaaag
     8821 agggcgactc catcggcgcg aaagtcaccg ttgttgccag tggtgtcccc gccggacttg
     8881 gcgagccggt ctttgaccgc ctggatgccg acatcgccca tgcgctgatg agcatcaacg
     8941 cggtgaaagg cgtggaaatt ggcgacggtt ttgacgtagt ggcgctgcgc ggcagccaga
     9001 accgcgacga aatcaccaaa gacggtttcc agagcaacca tgcgggcggc attctcggcg
     9061 gtatcagcag cgggcagcaa atcattgctc atatggcgct gaaaccgacc tccagcatta
     9121 ccgtgccggg gcgcactatt aaccgctttg gcgaagaagt tgagatgatc accaaaggcc
     9181 gtcacgatcc ctgtgtcggg atccgtgcag tgccgatcgc tgaagcgatg ctggcgatcg
     9241 ttttaatgga tcacctgtta cggcagcggg cgcaaaatgc cgatgtgaag actgatattc
     9301 cacgctggta aaaaatgaac aaaaccgcga ttgcgctgct ggctctgctt gccagtagcg
     9361 ctagcctggc agcgacgccg tggcaaaaaa taacccaacc tgtgccgggt agcgcacaat
     9421 cgataggcag tttttctaat ggctgtattg tcggcgctga cacgttgccg atacagtccg
     9481 aacattatca ggtcatgcgt accgatcagc gtcgctattt cggtcacccg gatctggtga
     9541 tgtttatcca gcgtctgagt agccaggtga gcaatctggg catgggtacg gtgctgattg
     9601 gcgatatggg gatgcccgct ggtgggcgtt tcaacggcgg tcatgccagc caccagaccg
     9661 gactggatgt cgatatcttt ctgcaactgc cgaaaactcg ctggacctcc gcgcagctct
     9721 tgcgcccgca agcactggac ttagtttccc gcgacggtaa acacgttgtc tccacgctgt
     9781 ggaagccaga aattttcagc ttgatcaaac tcgctgccca ggacaaagac gtcacgcgca
     9841 tttttgttaa tccggcgatt aaacaacaac tttgccttga tgcgggcacc gatcgcgact
     9901 ggttgcgcaa agtgcgaccc tggttccagc atcgcgcgca tatgcatgta cgattacgtt
     9961 gccctgccga tagtctggag tgtgaagatc aacctttacc gccatcaggc gatggttgcg
    10021 gggcagaact gcaaagctgg tttgcacctc taaaaccggg aacaacaaag cctgagaaga
    10081 agacaccgcc tccgttgccg ccttcctgcc aggcgctact ggatgagcac gtgatctaat
    10141 ggaaacgttt aatagcctgt ttatggtttc cccgctgttg ctgggagttc tcttttttgt
    10201 cgccatgctg gcgggattta tcgactcgat tgccggtggc ggtgggttac tcaccattcc
    10261 ggcattgatg gctgcgggaa tgtctcccgc taacgcgctg gcaaccaata aactgcaagc
    10321 ctgcggcggc tctatttccg ccactatcta ctttattcgc cgcaaagtgg ttagtttaag
    10381 cgatcagaaa ctcaatattg ccatgacctt tgtcggctca atgagcggcg cactgttggt
    10441 acagtacgtt caggctgatg tcttacggca gattttgccc attctggtga tttgtatcgg
    10501 cctctatttc ttgctgatgc caaagctggg tgaagaagat cgccagcgcc ggatgtacgg
    10561 tttacccttc gcgttaattg ccggtggctg cgtcggtttt tatgacggat tctttggccc
    10621 ggctgccgga tcgttttacg cgctggcctt cgttacgctg tgcggattca acctcgccaa
    10681 agccacggct cacgccaaat tactcaacgc aacgtcaaac attggcggtc tgctgctgtt
    10741 tattctcggc ggcaaagtga tttgggcgac gggctttgtc atgctggtgg ggcagttcct
    10801 cggcgcgcgc atgggttcac ggctggtgtt gagcaaagga caaaagctga tccgcccgat
    10861 gattgttatc gtctcggcgg tgatgagtgc caaattactt tacgatagcc acggacagga
    10921 gatcctccac tggttgggga tgaactaatg aacagtacac accactatga gcagttgatt
    10981 gaaattttta atagctgctt tgccgatgaa tttaataccc gtctgattaa aggcgacgac
    11041 gaaccgatct atcttcctgc tgatgcggaa gtaccgtata accgaattgt ctttgcccac
    11101 ggcttttatg ccagcgcaat tcatgagatt tcgcactggt gtattgccgg aaaagcgcgc
    11161 cgtgagctgg ttgatttcgg ctactggtat tgcccggatg ggcgtgatgc ccaaacgcaa
    11221 agccagtttg aagatgttga agtgaagccg caggcactcg actggttgtt ctgtgtggcg
    11281 gcgggatatc cgtttaacgt cagttgcgac aatctggaag gcgacttcga accggatcgt
    11341 gttgtgtttc aacggcgggt acatgcgcag gtgatggatt atctgacaaa tggcattccc
    11401 gagcgtccgg cacgcttcat taaagcacta caaaattatt atcacacgcc ggaacttacg
    11461 gcggaacagt tcccgtggcc ggaagcgctc aactgaagcc ggtgccacgt tttacaatga
    11521 ggaaagaaga tgatcgcgga gtttgaatca cgcattctgg cattaatcga cggcatggtt
    11581 gaccatgcca gtgatgatga gttgtttgcc agtgggtatt tgcgtggcca cctgacatta
    11641 gccatcgcag aactggaaag tggtgatgac cactccgctc aggcggtgca tacgaccgtt
    11701 agccagagtc tggaaaaagc cattggtgcc ggtgaattgt cgccgcgtga ccaggcgctg
    11761 gtgaccgata tgtgggaaaa cctgtttcag caggcgtcac agcagtaagt tgcggcgtct
    11821 ggagcaccat gcctgatgcg acgcttatcg cgtcttatca ggcctacacg tcacacgtag
    11881 gtcggataag gcgttcacgc cgcatccggc aaacactgcg agagcagatt accccgcctt
    11941 aaccgcttta cccttcaaca atttccgcac ccataaccga ttcgggttta acgccgtcag
    12001 cgtactggca tccatcggaa tcggttcgtc gctcatctgc gccgccagaa tctcggcaca
    12061 cagcggggca gaacacaaac cgcgagaacc taaagccgca aacataaaga gatcgtcaaa
    12121 aaccggcgcg cttaccgcct catctttctg ctccgccaac gacgcatatt ccacgagtgt
    12181 tgcctcataa tcgggaacat tgcctaccat tggcagatga tcgcgggtgg cacaacgcac
    12241 accgcagcgc gcctctttat cactgacatc aacctctttt gcccactgtg cctgcgggaa
    12301 acaatcaatc aaccgctggc gattctgctg ctgatcgtcc tcactgtacg ccgtatcttc
    12361 gctgccgcga tgataactgg caccaataca atgatgttga ttcgccggat tttgtggcgt
    12421 gagataaccg tcatagcaca gcacctgctt cagctctgcc agttccggcg ttgtcggaat
    12481 atggctgacc tgcccggcaa ccgaatacac cgggagagtc gacgtttggc tgaaacggct
    12541 gatttgatgc ccgttcgcca gtaccactac gctgtgtgtt gcttgctgat ctcctgcaaa
    12601 attcaacaac caacagtcat ccttacggga taaattctgt aactgatatt gataataaat
    12661 ctgcaaaccc tgctgttgcg ccagttccag cacattacgg gtcagttctg ctgggcacag
    12721 ccaaccacct tgcggataag taatgccgct gcaatttgtc gcaacgcccg taatttgttc
    12781 aaccgcattt gcctcaacgg ctacagccag ttctgcgggt aaatccattg acaacatctg
    12841 tgcgatttta tgctggcttt tctcatccca gcctaactgc gtgacgccgc accagtcatg
    12901 atcaaattta acgggtaatt ggtcgtaaaa ccgacgagca aaagtaaacg cattagagaa
    12961 aaagcggttt agcgcctcat cgtgtttgct taataacgga tacagcgccc cctggcgatt
    13021 gccggaagca cccagcgcgg gggcttcatc cgcgcaataa agcgctacct gccagccgcg
    13081 ccgtaataac gccagcgaca acaacgcgct ggcaatacca ccgccgataa tcgctacttc
    13141 ccgtttgctg ctgcccgtac gattaaacca cggcgtggag caggggagtg gtaatgtctg
    13201 ttccatcacc ccacaaagca tttcccgttt gcgcccaaag cccttacgtt tttgcatcgt
    13261 gaatccggct tcctgcaaac cgcggcggac aaaaccggca gacgtaaatg tcgccagcgt
    13321 gccgcctgga cgcgccaacc ttgccatggc gttaaacaga ttttgcgtcc acatatccgg
    13381 gtttttagct ggcgcaaagc cgtccagaaa ccaggcatct actttttgat ttagcgaatc
    13441 gtccagttgg ctggtcagtt cgttaatatc gccaaaccat aaatccagcg tcacgcggcc
    13501 ttcattgagc aataaacgat ggcaaccggg caagggcatt ggccactgcg cctgaagttg
    13561 ttctgcccac ggagccagtt ccggccagtg ttgatgcgct aaggctaaat ccgcacgggt
    13621 gaggggaaat ttctcaaaac taatgaaatg taagcgttgt aattgcgctt gcggatgcgc
    13681 ttcgcgaaac tgatcaaatg cctgccatag cgtcaggaag tttaatccgg tgccgaagcc
    13741 gctctctgct accacaaaca gaggatgtgg atgctcagga aagcgtacct ctaattggtt
    13801 gcctcccaga aaaacataac gcgtctcttc cagcccgtta tcgttggaaa aatagacatc
    13861 gtcaaaatct cgggaaacag gtgtaccctc agcattaaat tcgaggttgg caggttgtat
    13921 ggagtagtgt ttcacgtaag ttactcgtct tacaggcggt ggctcgatct tagcgatgtg
    13981 tgtaaggctg cgcaaatttc tctattaaat ggctgatcgg acttgttcgg cgtacaagtg
    14041 tacgctattg tgcattcgaa acttactcta tgtgcgactt acagaggtat tgaatgaaac
    14101 gtgcagtgat tactggcctg ggcattgttt ccagcatcgg taataaccag caggaagtcc
    14161 tggcatctct gcgtgaagga cgttcaggga tcactttctc tcaggagctg aaggattccg
    14221 gcatgcgtag ccacgtctgg ggcaacgtaa aactggatac cactggcctc attgaccgca
    14281 aagttgtgcg ctttatgagc gacgcatcca tttatgcatt cctttctatg gagcaggcaa
    14341 tcgcagatgc gggcctttct ccggaagctt accagaataa cccgcgcgtt ggcctgattg
    14401 caggttctgg cggcggttcc ccgcgtttcc aggtattcgg cgctgacgca atgcgcggcc
    14461 cgcgcggcct gaaagcggtt ggcccgtatg tggtcaccaa agcgatggca tccggcgttt
    14521 ctgcctgcct cgccacgccg tttaaaattc atggcgttaa ctactccatc agctccgcgt
    14581 gtgcgacttc cgcacactgt atcggtaacg cagtagagca gatccaactg ggcaaacagg
    14641 acatcgtgtt tgctggcggc ggcgaagagc tgtgctggga aatggcctgc gaattcgacg
    14701 cgatgggtgc gctgtctact aaatacaacg acaccccgga aaaagcctcc cgtacttacg
    14761 acgctcaccg tgacggtttc gttatcgctg gcggcggcgg tatggtagtg gttgaagagc
    14821 tggaacacgc gctggcgcgt ggtgctcaca tctatgctga aatcgttggc tacggcgcaa
    14881 cctctgatgg tgcagacatg gttgctccgt ctggcgaagg cgcagtacgt tgtatgcaga
    14941 tggcgatgca tggcgttgat accccaatcg attacctgaa ctctcacggt acttcgactc
    15001 cggttggcga cgtgaaagag ctggcagcta tccgtgaagt gttcggcgat aagagcccgg
    15061 cgatttctgc aaccaaagcc atgaccggtc actcactggg cgctgcgggt gtgcaggaag
    15121 ctatctactc tctgctgatg ctggaacacg gctttatcgc cccgagcatc aacattgaag
    15181 agctggacga gcaggctgcg ggtctgaaca tcgtgaccga aacgaccgat cgcgaactga
    15241 ccactgttat gtctaacagc ttcggcttcg gcggcaccaa cgccacgctg gtaatgcgca
    15301 agctgaaaga ttaattcgca gtaggccgga gtaggtcgga taagacgcgc cagcgtcgca
    15361 tccgacgtta cgcgccaatg cggcctccgg cactaacgca aaagggaacc tgatggttcc
    15421 ctttttcaca tcattgacaa tcgccaccag ttccaggcaa acttcccgct ttgtcgattt
    15481 ccttctgaaa agacgtacgc gttaaatcct gccaacgcac cgtaaccctg aaaccagaga
    15541 gatgagacgg ggatactcct cgccttgcgc tgcattctgg agtaatgcat gactgctgta
    15601 agccaaaccg aaacacgatc ttctgccaat ttttcgctct tccgcatcgc ttttgcggtt
    15661 tttctcacct acatgaccgt agggctgccg ttgccggtta tcccgctgtt tgttcatcat
    15721 gaactgggct atggcaatac catggtcggc attgccgtcg ggattcagtt tctggctacg
    15781 gtgctgacgc gtggctatgc cgggcgactg gccgatcaat atggcgcaaa acgttcggcg
    15841 cttcagggga tgttagcttg tggtctggct ggcggcgcgt tgctgctggc ggcgattttg
    15901 cctgtctccg caccgttcaa atttgccctg ttggtcatcg ggcgtttgat tcttgggttt
    15961 ggtgaaagcc agttactgac aggcgctctg acctgggggt taggcatcgt agggccaaaa
    16021 cactctggca aagtgatgtc atggaatgga atggcgattt acggtgccct cgctgttggt
    16081 gctccgcttg gcctgttgat tcatagccat tacggttttg ccgcactggc gatcaccaca
    16141 atggtattac ccttactggc gtgggcctgt aacggcacag tgcgcaaagt accggccctg
    16201 gcgggagaac gtccatcgct gtggagcgtt gtcgggttta tctggaaacc agggttaggg
    16261 ctggcactac aaggcgttgg ttttgcggtt atcgggactt tcgtttcgct ctactttgcc
    16321 agcaaaggat gggcgatggc gggctttact cttaccgagt ttggcggcgc atttgtcgtg
    16381 atgcgcgtca tgtttggctg gatgccggac cgttttggcg gcgtgaaagt ggcgattgtc
    16441 tctctgcttg tagaaacggt gggcttgttg ctgctctggc aagccccagg tgcgtgggtc
    16501 gcattagcgg gcgcggcgtt aaccggagcc ggatgttcgc ttatctttcc tgcgctgggc
    16561 gtggaggtgg ttaaacgcgt cccctcacaa gttcgcggca ccgcactggg cggttacgcc
    16621 gcgtttcagg atatcgccct cggcgtctcc gggccgctgg cgggaatgct ggcgaccacg
    16681 tttggttact cttcggtatt tcttgccggg gcgatctctg cggtgctggg aattattgtc
    16741 acgatactgt catttcgtcg gggctaacga accagccaga ccagcatcag gataatcgcg
    16801 actaacagta tccacagcgc gggacgcgtt gccatatttt gcaacgtgtc catcatcggt
    16861 ggaaaaggat taagcagcgg ttgcatatca cgcggatcaa tccctttaac ccgccgttga
    16921 taaagctgat taagaatatc ctcggcctgg gcagaagaaa gcgatgattg cgcagaaaga
    16981 ccatattttt gctggatata cgccgataac gctgccagtt cactggcatc taaaggttgt
    17041 tttagcgcca tctgtagtga ttccagcgtc ggcgtatttt gctggcttaa cgtctggcgc
    17101 gcctgcaacc aggtcaccag atggttaaac agtttcgctg gaattaactc gccatcgttc
    17161 accccggaaa gttccagcat cgattgccag atctgtttgc tgggttcccc cgttgccgcc
    17221 gcaagtttgg tcaccagctg ttttagcgca ttgtgctccg ccggtaataa aggacggtcg
    17281 gtcgcctcgc gctgctgtgg ttgcggaata accatcttcc cttcctgcaa cagggtgaga
    17341 atggttttta attgctccgg tgagagctga ttcagcggcg tctgaccaaa gttatgacgg
    17401 atataatccg tgaccgcctg acgattattc cccagacgta aatactcccc taattgcgct
    17461 aaaagctggc gggcagaatg gcttttttgc gcggccagca gacgttgcgc cagattatgc
    17521 tcagcggcag ggaagtgacg cgaaagcagc ggtgaatctc ccgacagacc aatatcgtgc
    17581 ctgatacccg cccacagttc tgctctttgt tgttgcgtca gtgaggtcac tttcgtcatt
    17641 aagctttcca gcgaagtacg ttgctgactg gataaaggct gattgcccgc gccagacggc
    17701 aggttatctc cctgacctgg tggttgccca ggaggagggc cggaaatagg ttgtatcatt
    17761 acgtatcctt atacctgaaa tcttcgcaag tatgcctggc cgcgagatta tggcacactt
    17821 gtccggttaa ctctcgtctc atacaggtaa cacaaacgtg aaaatccttg ttgatgaaaa
    17881 tatgccttat gcccgcgact tatttagccg tttgggtgag gtgaccgcgg ttcccgggcg
    17941 tccaatcccc gtcgctcaac tggcagacgc ggatgcgctg atggtgcgtt cggtcacgaa
    18001 agtgaatgaa tctttgctgg caggaaaacc cattaaattt gttggcactg ccactgcggg
    18061 gaccgaccat gtcgatgaag catggttgaa gcaggcggga attggttttt ccgctgcacc
    18121 tggctgtaat gcgattgcgg tggtggaata tgttttctcc tcactactga tgcttgccga
    18181 acgcgatgga ttttcactgc acgaccgtac ggtggggatc gtgggcgttg gtaacgttgg
    18241 acgtcgattg caggcgcgac tggaagcgtt agggattaaa acattgcttt gcgatccgcc
    18301 tcgcgctgac cgtggggatg agggtgattt ccgctcgctg gatgaattag ttcagcatgc
    18361 ggatattctg actttccata cgccactctt caaagatggg ccgtacaaaa cgctgcatct
    18421 ggcggatgaa aaactgatcc gtagcctgaa gccgggagcg attctgatta acgcctgccg
    18481 tggcgcagtc gtcgataata ctgcgttgct gacctgcctg aatgaaggcc agaagttaag
    18541 cgtagtgctg gatgtctggg aaggcgaacc ggaacttaac gtggagttgc tgacaaaagt
    18601 ggatatcggc acgccgcata tcgcaggtta taccctggaa ggtaaagcac gcggtactac
    18661 gcaagtgttt gaagcctata gcaagtttat tgggcatgaa cagcacgttg cgctggatac
    18721 attactgccc gcgccagagt ttggtcgcat tacgctgcat ggcccgctcg atcaaccgac
    18781 gctgaaaagg ctggtgcatt tggtgtatga tgtgcgccgc gatgacgcac cgctgcgtaa
    18841 agtcgccggg ataccgggtg agttcgataa actgcgcaaa aactatcttg agcgccgtga
    18901 atggtcatct ctgtatgtaa tttgtgatga cgccagtgcg gcatcattgc tgtgtaaact
    18961 gggttttaac gccgttcatc atccggcacg ttaatctctt cttcatgctc tctgctgtaa
    19021 cattggcagg gagctttgct atttctggag taaaccacca tgtctgaagg ctggaacatt
    19081 gccgtcctgg gcgcaactgg cgctgtgggc gaagccctgc ttgaaacgct ggctgaacgt
    19141 cagttcccgg ttggggaaat ttatgcactg gcacgtaacg aaagcgcagg cgaacaactg
    19201 cgctttggtg gtaaaacaat cactgtgcag gatgccgctg aattcgactg gacgcaggcg
    19261 cagctggcat tttttgtcgc aggcaaagaa gctaccgctg cctgggttga agaagcgacc
    19321 aactcaggtt gcctggtgat cgacagcagc ggactgtttg ctctcgaacc cgacgtaccg
    19381 ctggtggtgc cggaagtaaa cccgtttgta ctgaccgatt accgcaaccg gaatgtcatc
    19441 gccgtaccag acagtctgac cagccagctg ctggcggcac tgaaaccgtt aatcgatcag
    19501 ggcggtttgt cgcgtatcag tgttaccagc ctgatttcag cctccgccca gggcaaaaaa
    19561 gcggtcgatg cgttagcggg gcagagtgcg aaattgctca acggcattcc gattgacgaa
    19621 gaagatttct tcggtcgtca actggcgttc aatatgctgc cgttgttgcc ggatagcgaa
    19681 ggtagcgtgc gtgaagaacg tcgtatcgtt gacgaagtac gcaaaatcct gcaggacgaa
    19741 gggctgatga tttcggcaag cgtcgtccag gcaccggtat tctacggtca tgcccagatg
    19801 gtcaactttg aagcactgcg tccgctggcg gcagaagaag cgcgtgatgc gtttgctcag
    19861 ggcgaagata ttgtgctctc tgaagagaac gaattcccga ctcaggtagg ggatgcttcg
    19921 ggtacgccgc atctttctgt tggctgcgtg cgtaatgact acggtatgcc ggagcaagtc
    19981 cagttctggt cggtggccga taacgttcgc tttggcggcg cgctgatggc agtaaaaatc
    20041 gccgagaaac tggtgcagga gtatctgtac taatgtccga ccagcaacaa ctgccagttt
    20101 ataaaattgc gctgggcatt gagtacgacg gcagtaagta ttacggctgg caacggcaga
    20161 atgaagtccg cagtgtgcag gagaagctgg aaaaggcgct ctcccaggtg gcgaacgaac
    20221 ccatcaccgt cttctgcgcc gggcgtactg acgcaggggt acacggtacc gggcaggtgg
    20281 tgcatttcga aacaaccgcg cagcgcaaag acgcggcgtg gaccttgggc gtaaatgcga
    20341 atttacctgg tgacatcgcc gtgcgttggg ttaaagctgt acctgatgat tttcatgccc
    20401 gatttagcgc cacggctcgc cgttatcgct acatcatcta caatcatcgg ctgcgcccgg
    20461 cggtattgag taaaggggta acccattttt acgaaccgct ggacgctgaa cggatgcatc
    20521 gggctgcgca atgcttgctg ggcgagaatg atttcacctc gttccgtgcg gtgcagtgcc
    20581 agtcccgaac cccgtggcgc aacgttatgc atattaatgt cacgcgtcac ggtccttatg
    20641 tggtggtaga tatcaaagcg aatgcctttg tacatcatat ggtcaggaat attgtcggca
    20701 gcctgatgga agtcggtgcc cacaaccagc cggagagatg gatagcagag ttgctggcgg
    20761 caaaggacag aacgctggcg gcagcaacgg caaaagcgga agggctgtat ctggtcgcgg
    20821 tggattaccc tgaccggtat gatcttccaa aaccgccaat gggcccgcta tttctggcgg
    20881 actaacgaga ataatgctcg gaacatttca ggctgtagcc aacgtagtta cagcctgaaa
    20941 gatgacgagt acaaggcata ggcaaatatg gacctgattt atttcctcat cgattttatc
    21001 ctgcacattg acgtgcatct ggcagagctg gtcgcggagt acggcgtctg ggtttatgcc
    21061 attctgtttt tgattctgtt ctgcgaaacg gggctggtgg taacgccatt cctgcctggt
    21121 gattcgctgc tgtttgttgc tggtgcattg gcgtcgctgg aaactaacga tctcaatgtc
    21181 catatgatgg tggtgcttat gttgattgcc gctattgtcg gtgatgcagt gaactatacc
    21241 attggacggt tatttggtga caaactgttc agtaacccgg attcgaaaat ctttcgtcgc
    21301 agttatcttg ataagactca tcagttttac gagagacatg gcggcaaaac gattattctc
    21361 gcccgttttg taccgatcgt cagaacgttc gcgccgtttg ttgcgggaat gggccacatg
    21421 tcgtaccgtc atttcgccgc ttataacgtg atcggcgcac tgttgtgggt actgattttt
    21481 acctacgcgg gttatttctt cggcacgctc cccttcattc agagcaacct taagctgatg
    21541 attgttggca ttatttttgt ttcaatattg cctggggttt tcgaatttat tcgtcacaaa
    21601 cgcgctgcgg cacgcgccgc aaaataaaaa gtaactccgc ggttcgacca cttttttatc
    21661 caaagtttcg ggctgttatg ttttaatgtg caacattcat ggtctgttgg gggcaaaaat
    21721 ggcattatgc gtccccaaag ataaaactgg catcgaacca ggttcagaca gaaaggtccc
    21781 taatgagctg gattgaacga attaaaagca acattactcc cacccgcaag gcgagcattc
    21841 ctgaaggggt gtggactaag tgtgatagct gcggtcaggt tttataccgc gctgagctgg
    21901 aacgtaatct tgaggtctgt ccgaagtgtg accatcacat gcgtatgaca gcgcgtaatc
    21961 gcctgcatag cctgttagat gaaggaagcc ttgtggagct gggtagcgag cttgagccga
    22021 aagatgtgct gaagtttcgt gactccaaga agtataaaga ccgtctggct tctgcgcaga
    22081 aagaaaccgg cgaaaaagat gcgctggtgg tgatgaaagg cactctgtat ggaatgccgg
    22141 ttgtcgctgc ggcattcgag ttcgccttta tgggcggttc aatggggtct gttgtgggtg
    22201 cacgtttcgt gcgtgccgtt gagcaggcgc tggaagataa ctgcccgctg atctgcttct
    22261 ccgcctctgg tggcgcacgt atgcaggaag cactgatgtc gctgatgcag atggcgaaaa
    22321 cctctgcggc actggcaaaa atgcaggagc gcggcttgcc gtacatctcc gtgctgaccg
    22381 acccgacgat gggcggtgtt tctgcaagtt tcgccatgct gggcgatctc aacatcgctg
    22441 aaccgaaagc gttaattggc tttgccggtc cgcgtgttat cgaacagacc gttcgcgaaa
    22501 aactgccgcc aggattccag cgcagtgaat tcctgatcga gaaaggcgcc atcgacatga
    22561 tcgtccatcg tccggaaatg cgcctgaaac tggcgagcat tctggcgaag ttgatgaatc
    22621 tgccagcgcc gaatcctgaa gcgccgcgtg aaggcgtagt ggtacccccg gtaccggatc
    22681 aggaacctga ggcctgataa ctgataaggg cagggccact ggctctgccc ttttgctatt
    22741 ctcaccgtaa cgaatcagcg gataccatga ttatcaaacg cactcctcaa gccgcgtcgc
    22801 ctctggcttc gtggctttct tatctggaaa acctgcacag taaaactatc gatctcggcc
    22861 ttgagcgcgt gagccaggtc gcggcgcgtc ttggcgtcct gaaaccagcg ccatttgtgt
    22921 ttaccgttgc gggtacgaat ggcaaaggca ccacctgccg tacgctggag tcgattctga
    22981 tggcggcagg gtacaaagtg ggcgtctaca gttcaccgca tctggtgcgt tataccgagc
    23041 gcgtacgtgt gcaggggcag gaattgccgg aatcggccca caccgcctct tttgcggaga
    23101 ttgaatcggc acgcggtgat atttccctga cctatttcga gtacggtacg ctgtcggcgt
    23161 tgtggctgtt caaacaggca caacttgacg tagtgattct ggaagtaggg ctgggcggtc
    23221 gtctggacgc aaccaatatt gtcgatgccg atgtggctgt agtaaccagc attgcgctgg
    23281 atcataccga ctggctggga ccagatcgcg aaagtattgg tcgcgagaaa gcaggcatct
    23341 ttcgcagcgc aaaaccggca attgtcggtg agccggaaat gccttctacc attgctgatg
    23401 tggcgcagga aaaaggtgca ctgttacaac gtcggggcgt tgagtggaac tattccgtca
    23461 ccgatcatga ctgggcgttt agcgatgctc acggcacgct ggaaaatctg ccgttgccgc
    23521 ttgtcccgca accgaatgcc gcaacagcgc tggcggcact gcgtgccagc gggctggaag
    23581 tcagtgaaaa tgccattcgc gacgggattg ccagcgcaat tttgccggga cgtttccaga
    23641 ttgtgagcga gtcgccacgc gttatttttg atgtcgcgca taatccacat gcggcggaat
    23701 atctcaccgg gcgtatgaaa gcgctaccga aaaacgggcg cgtgctggcg gttatcggta
    23761 tgctacatga taaagatatt gccggaactc tggcctggtt gaaaagcgtg gttgatgact
    23821 ggtattgtgc gccactggaa gggccgcgcg gtgccacggc agaacaactg cttgagcatt
    23881 tgggtaacgg caaatcattt gatagcgttg cgcaggcatg ggatgccgca atggcggacg
    23941 ctaaagcgga agacaccgtg ctggtgtgtg gttctttcca cacggtcgca catgtcatgg
    24001 aagtgattga cgcgaggaga agcggtggca agtaagtttc agaatcggtt agtgggcacg
    24061 atcgtgctgg tggcgctggg ggtgattgta cttccagggc tgctggacgg gcagaaaaaa
    24121 cattatcagg atgagtttgc ggctatcccg ctggttccaa aagcgggcga tcgcgatgag
    24181 cctgatatga tgccagccgc cacccaggcg ttgccgacgc agccgccgga aggcgcagcg
    24241 gaagaggtgc gggcaggtga tgccgcagca ccgtcgctcg atccggccac tattgcagcc
    24301 aataacaccg agtttgaacc ggaacctgca ccggtcgccc caccgaagcc gaaaccggtg
    24361 gagccgccta aaccgaaggt tgaagcacca cctgcgccga agccagaacc gaagccggtc
    24421 gtggaagaaa aagcagcgcc aacgggtaaa gcctatgttg tgcaactggg tgcgctgaaa
    24481 aatgccgata aagtgaatga gattgtcggt aagctgcgcg gtgccggtta tcgggtttat
    24541 acgtcgccat ccacgccagt gcagggtaaa attacccgta ttctggttgg gccggatgcc
    24601 tcgaaagata agctgaaagg ttcgctgggt gagttgaagc aactttctgg cttaagtggc
    24661 gtggtaatgg gctatacgcc gaattaatac agtcttgcct gatgcgacgc tggcgcgtct
    24721 tatcaggcct gggcagtgtt agaaccgtag gtcggataag gcgtttacgc cgcatccgac
    24781 acgcattgca cgatgccgca aaggcataaa aagtcgatgg cgttgaatat tttttcagcg
    24841 ccatttttat tgatgcgtgg gaaggaaatc cctacgcaaa cgttttcttt ttctgttaga
    24901 atgcgccccg aacaggatga cagggcgtaa aatcgtggga cacatatggt ctggattgat
    24961 tacgccataa tcgcggtgat tgctttttcc tctctggtta gcctgatccg cggctttgtt
    25021 cgtgaagcgt tatcgctggt gacatggggt tgtgctttct ttgttgccag tcattactac
    25081 acttacctgt cagtctggtt tacgggcttt gaagacgaac tggttcgaaa tggaattgcc
    25141 atcgcggtac tgtttatcgc taccctgatt gttggtgcta tcgtgaactt cgtgataggc
    25201 cagttggtgg agaaaacggg gttgtcaggc accgatcggg tgctgggcgt ctgtttcggt
    25261 gcgttgcgcg gtgtgttgat tgttgccgcc attctcttct ttctcgactc ctttaccggg
    25321 gtgtcgaaaa gcgaagactg gagcaaatca cagctgatcc cgcagttcag ttttatcatc
    25381 agatggtttt ttgattatct gcaaagctcg tcaagtttct tgcccagagc gtaagtgctc
    25441 tgagatgtgg cttaacgagg aaaaagacgt atgtgcggta ttgtcggtat cgccggtgtt
    25501 atgccggtta accagtcgat ttatgatgcc ttaacggtgc ttcagcatcg cggtcaggat
    25561 gccgccggca tcatcaccat agatgccaat aactgcttcc gtttgcgtaa agcgaacggg
    25621 ctggtgagcg atgtatttga agctcgccat atgcagcgtt tgcagggcaa tatgggcatt
    25681 ggtcatgtgc gttaccctac ggctggcagc tccagcgcct ctgaagcgca gccgttttac
    25741 gttaactccc cgtatggcat tacgcttgcc cacaacggca atctgaccaa cgctcacgag
    25801 ttgcgtaaaa aactgtttga agaaaaacgc cgccacatca acaccacttc cgactcggaa
    25861 attctgctta atatcttcgc tagcgagctg gacaacttcc gccactaccc gctggaagcc
    25921 gacaatattt tcgctgccat tgctgccaca aaccgcttaa tccgcggcgc atatgcctgt
    25981 gtggcgatga ttatcggcca cggtatggtt gctttccgcg atccaaacgg gattcgtccg
    26041 ctggtactgg gaaaacgtga tattgacgag aaccgtacag aatatatggt cgcttccgaa
    26101 agcgtagcgc tcgatacgct gggctttgat ttcctgcgtg acgtcgcgcc gggcgaagcg
    26161 atttacatca ctgaagaagg gcagttgttt acccgtcaat gtgctgacaa tccggtcagc
    26221 aatccgtgcc tgtttgagta tgtatacttt gctcgcccgg actcgttcat cgacaaaatt
    26281 tccgtttaca gcgcgcgtgt gaatatgggc acgaaactgg gcgagaaaat tgcccgcgaa
    26341 tgggaagatc tggatatcga cgtggtgatc ccgatcccag aaacctcgtg tgatatcgcg
    26401 ctggaaattg ctcgtattct gggcaaaccg taccgccagg gcttcgttaa aaaccgctat
    26461 gttggccgca cctttatcat gccgggccag cagctgcgtc gtaagtccgt gcgccgtaaa
    26521 ctgaatgcca accgcgccga gttccgcgat aaaaacgtcc tgctggtcga cgactccatc
    26581 gtccgtggca ccacttctga gcagattatc gagatggcac gcgaagccgg agcgaagaaa
    26641 gtgtacctcg cttctgcggc accggaaatt cgcttcccga acgtttatgg tattgatatg
    26701 ccgagcgcca cggaactgat cgctcacggt cgcgaagttg atgaaattcg ccagatcatc
    26761 ggtgctgacg ggttgatttt ccaggatctg aacgatctga tcgacgccgt tcgcgctgaa
    26821 aatccggata tccagcagtt tgaatgctcg gtgttcaacg gcgtctacgt caccaaagat
    26881 gttgatcagg gctacctcga tttcctcgat acgttacgta atgatgacgc caaagcagtg
    26941 caacgtcaga acgaagtgga aaatctcgaa atgcataacg aaggatgatg ccttcgctga
    27001 gggtgccggt ctggcaccct gacttgcaac tcccgccgaa atactgcaaa gtctgcctgc
    27061 aagtctgaca gggcaactat ttatgaaacg actcattgta ggcatcagcg gtgccagcgg
    27121 cgcgatttat ggcgtgcgct tattacaggt tctgcgcgat gtcacagata tcgaaacgca
    27181 tctggtgatg agccaggcgg cgcgccagac cttatccctc gaaacggatt tttccctgcg
    27241 cgaagtgcag gcattagctg atgtcacgca cgatgcgcgc gatatttccg ccagcatctc
    27301 ttccggttct ttccagacgc tggggatggt gattttaccc tgttcaatca aaaccctttc
    27361 cggcattgtc catagctata ctgatggctt actgacccgt gcggcagatg tggtgctgaa
    27421 agagcgtcgc ccgttggtgc tctgcgtgcg tgaaacacca ttgcacttag gccatctgcg
    27481 tttaatgact caggcggcag aaatcggtgc ggtgattatg cctcccgttc cggcgtttta
    27541 tcatcgcccg caatcccttg atgatgtgat aaatcagacg gttaatcgtg ttcttgacca
    27601 gtttgcaata acccttcctg aagatctctt tgcccgctgg cagggcgcat aactccgctg
    27661 ttgccctgtt tcagggcaat tttgcaaccg cgatcaaatc ctcgacattt tgtttctgcc
    27721 attcaatcga aacgctgcga ttcaaccgct atacctgcta tcttcaactt caggacaata
    27781 atgcaacgtc ttattaacat atttaacgtt gaatgttact gttgtcgtca agatggcata
    27841 agacctgcat gaaagagcct gcaaacacac aacacaatac acaacataaa aaagccattt
    27901 tcacttgagg gttatgtatg aagaagtcga ttctcgctct gtctttgtta gtcggtctct
    27961 ccgcagcggc ttccagctat gcggcgctac cggagacggt acgtatcgga accgatacca
    28021 cctatgcgcc gttctcgtcg aaagatgcta aaggtgattt tgttggcttt gatatcgatc
    28081 tcggtaacga gatgtgcaaa cggatgcagg tgaaatgtac ctgggttgcc agtgactttg
    28141 acgcgctgat cccctcactg aaagcgaaaa aaatcgacgc cattatttcg tcgctttcca
    28201 ttaccgataa acgtcagcag gagattgcct tctccgacaa gctgtacgcc gcagattctc
    28261 gtttgattgc tgccaaaggt tcaccgattc agccaacgct ggattcactg aaaggtaaac
    28321 atgttggtgt gctgcaggga tcaacccagg aagcttacgc taacgagacc tggcgtagca
    28381 aaggcgtgga tgtggtggcc tatgccaacc aggatttggt ctattccgat ctggctgcag
    28441 gacgtctgga tgctgcgtta caagatgaag ttgctgccag cgaaggattc ctcaagcaac
    28501 ctgctggtaa agctttcgcc tttgctggct catcagtaaa agacaaaaaa tacttcggtg
    28561 atggcaccgg tgtagggcta cgtaaagatg atgctgaact gacggctgcc ttcaataagg
    28621 cgcttggcga gctgcgtcag gacggcacct acgacaagat ggcgaaaaag tatttcgact
    28681 ttaatgtcta cggtgactga tacgtcgctg ggaagctgta cctgatggaa tgatcatcat
    28741 ggtgcacgcc aggtttgttg cactatcgtg gtgcattgaa atgcatactt aagcattttt
    28801 aatgaaaaat aatacgtcta acggggcgga atattttgcc ttgatggtca attttatggc
    28861 acgataagtg taacaaacct gtaaatattc cctataaaaa gactgtcagt tgaggacatt
    28921 atgaaaaaac tggtgctatc gctctctctg gttctggcct tctccagcgc aacagcggca
    28981 tttgctgcga ttccgcaaaa catccgcatc ggtaccgacc cgacctatgc gccatttgaa
    29041 tcaaaaaatt cacaaggcga actggttggc ttcgatatcg atctggcaaa ggaattatgc
    29101 aaacgcatca atacgcaatg tacgtttgtc gaaaatccgc tggatgcgtt aatcccgtcc
    29161 ttaaaagcga agaagattga cgccatcatg tcatcgcttt ccattacgga aaaacgtcag
    29221 caagaaatag ccttcaccga caaactgtac gctgccgatt ctcgtttggt ggtggcgaaa
    29281 aattctgaca ttcagccgac agtcgagtcg ctgaaaggca aacgggtagg cgtattgcag
    29341 ggcaccaccc aggagacgtt cggtaatgaa cattgggcac caaaaggcat tgaaatcgtc
    29401 tcgtatcagg ggcaggacaa catttattct gacctgactg ccggacgtat tgatgccgcg
    29461 ttccaggatg aggtcgctgc cagcgaaggt ttcctcaaac aacctgtcgg taaagattac
    29521 aaattcggtg gcccgtctgt taaagatgaa aaactgtttg gcgtagggac cggcatgggc
    29581 ctgcgtaaag aagataacga actgcgcgaa gcactgaaca aagcctttgc cgaaatgcgc
    29641 gctgacggta cttacgagaa attagcgaaa aagtacttcg attttgatgt ttatggtggc
    29701 taatcgccat cggtgaaaga aagcccgctc ccttcgggta accggaggga gacaagaggc
    29761 ggtaattcac cacacacgac aggacaggca gcatgttgta tgggttttca ggtgttattt
    29821 tacagggtgc gctcgtcacg ctggagctgg ctatcagctc tgtagtgctc gctgtaatca
    29881 tcggtttaat tggcgctggc ggtaagctct cgcaaaatcg gctttcgggg ctgattttcg
    29941 aagggtacac cacgctgatt cgtggcgtgc cggacttagt gttgatgctg ctgattttct
    30001 acggtttgca gattgcgcta aacacggtga cggaggcgat gggcgtcggg cagattgata
    30061 tcgatccgat ggtcgctggt attatcactc tcggttttat ctacggtgct tattttaccg
    30121 aaacgttccg tggtgctttt atggcagtgc cgaaaggaca tatagaggcg gcgacggcgt
    30181 tcggttttac tcgtgggcaa gtgtttcggc ggatcatgtt tccggcgatg atgcgttacg
    30241 ccctgccagg cattggcaac aactggcagg tgatcctcaa atctaccgca ctggtttcgt
    30301 tactcggcct ggaagatgtg gtcaaagcca cccaactggc aggcaaaagt acctgggaac
    30361 cgttctattt cgccatcgtc tgtggcgtga tttacctggt tttcaccact gtttccaatg
    30421 gtgtgctgct gttccttgag cgccgctact ccgtgggtgt gaagagggct gacctgtgat
    30481 cgaaatctta catgaatact ggaaaccgct gctgtggacc gacggttatc gctttactgg
    30541 tgtggcgatc actctgtggc tgcttatttt gtcggtagtg ataggcggag tcctggcgct
    30601 gtttctggcg attggtcgtg tctccagtaa taaatacatc cagtttccaa tctggttatt
    30661 tacctatatt tttcgcggta cgccgctgta tgttcagttg ctggtgttct attccggcat
    30721 gtacacgctt gagattgtta agggaaccga attccttaac gctttcttcc gcagtggcct
    30781 gaactgtacc gtgctggcgc tgacgcttaa cacctgcgct tacactaccg agatttttgc
    30841 tggggcaatc cgttcggttc cgcatgggga aattgaagcc gccagagcct atggcttctc
    30901 gacttttaaa atgtatcgtt gcattatttt gccttctgcg ctgcgtattg cgttaccggc
    30961 atacagcaac gaagtgatcc tgatgctgca ctctactgcg ctggcgttta cggccactgt
    31021 gccggatctg ctgaaaatag cccgcgatat taacgccgcc acgtatcaac cttttaccgc
    31081 cttcggcatt gccgcggtgc tctatttaat catctcttat gtcctgatca gcctctttcg
    31141 cagagcggaa aaacgctggt tgcagcatgt gaaaccttct tcaacgcact gagaatacga
    31201 tgtccgagaa taaattaaac gttatcgatt tgcacaaacg ctacggcgaa catgaagtgc
    31261 tgaaaggggt ctcactgcaa gcgaatgccg gagatgtaat aagcatcatc ggatcgtcgg
    31321 gatcggggaa aagtaccttt ctgcgctgca ttaacttcct cgaaaaaccg agtgaagggt
    31381 cgatcgtggt caatggccag acgatcaatc tggtgcgcga caaagacggt caactcaaag
    31441 tcgccgataa aaatcaactg cgcttactgc gcacacgcct gacgatggta ttccagcact
    31501 tcaatctctg gagccatatg acggtgctgg aaaacgtcat ggaagcgccg attcaggtgt
    31561 tgggcctgag caagcaggaa gcgcgcgagc gggcggtgaa gtatctggcg aaagtcggga
    31621 tagacgaacg tgcgcagggg aaatatccgg tgcatctttc cggcggtcag caacagcgtg
    31681 tttctatcgc gcgggcgctg gcgatggaac cggaagtttt actgtttgat gaacctacct
    31741 cggcgctcga tcctgaactg gtaggcgaag tgttacgtat tatgcagcaa ctggcagaag
    31801 aggggaaaac catggtggta gtgactcacg aaatgggctt tgctcgccat gtttctactc
    31861 acgtcatttt cctccatcag gggaaaatag aagaagaggg cgcgccggag cagttatttg
    31921 gcaacccgca aagccctcgt ctgcaacagt tccttaaggg atcgctgaaa taatttcctc
    31981 tccggtggct aacgctgccg gagtattttc atggggaatt tgcgagtcag cttccgctga
    32041 aatgtaaaag ttgcagtccg ctaaaaatac ttctgaattc gctgtccaaa gataaaaact
    32101 gcctgctgca ttgggtgtta atcgtggtta ttgtcgcgtc atcttaaatt tgaggggtga
    32161 cggaacgcca tgacaatatc gacaacttcc acgccgcatg atgcggtatt taaatctttt
    32221 ttacgccatc cag
//
LOCUS       DAZOYL010000012        31627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZOYL010000012 DAZOYL010000000
VERSION     DAZOYL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.012"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            156..491
                     /gene="eutS"
                     /locus_tag="ACHUOY_000411"
     CDS             156..491
                     /gene="eutS"
                     /locus_tag="ACHUOY_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR0681300.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            504..983
                     /gene="eutP"
                     /locus_tag="ACHUOY_000412"
     CDS             504..983
                     /gene="eutP"
                     /locus_tag="ACHUOY_000412"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR0681301.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            958..1659
                     /gene="eutQ"
                     /locus_tag="ACHUOY_000413"
     CDS             958..1659
                     /gene="eutQ"
                     /locus_tag="ACHUOY_000413"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR0681302.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1656..2459
                     /gene="eutT"
                     /locus_tag="ACHUOY_000414"
     CDS             1656..2459
                     /gene="eutT"
                     /locus_tag="ACHUOY_000414"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR0681303.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            2456..3472
                     /gene="pta"
                     /locus_tag="ACHUOY_000415"
     CDS             2456..3472
                     /gene="pta"
                     /locus_tag="ACHUOY_000415"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0681304.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3511..3804
                     /gene="eutM"
                     /locus_tag="ACHUOY_000416"
     CDS             3511..3804
                     /gene="eutM"
                     /locus_tag="ACHUOY_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR0681305.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3911..4198
                     /gene="eutN"
                     /locus_tag="ACHUOY_000417"
     CDS             3911..4198
                     /gene="eutN"
                     /locus_tag="ACHUOY_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR0681306.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4210..5613
                     /locus_tag="ACHUOY_000418"
     CDS             4210..5613
                     /locus_tag="ACHUOY_000418"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR0681307.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            5624..6460
                     /gene="eutJ"
                     /locus_tag="ACHUOY_000419"
     CDS             5624..6460
                     /gene="eutJ"
                     /locus_tag="ACHUOY_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR0681308.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6450..7637
                     /gene="eutG"
                     /locus_tag="ACHUOY_000420"
     CDS             6450..7637
                     /gene="eutG"
                     /locus_tag="ACHUOY_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR0681309.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7954..9180
                     /gene="eutH"
                     /locus_tag="ACHUOY_000421"
     CDS             7954..9180
                     /gene="eutH"
                     /locus_tag="ACHUOY_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR0681310.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            9177..10580
                     /gene="eutA"
                     /locus_tag="ACHUOY_000422"
     CDS             9177..10580
                     /gene="eutA"
                     /locus_tag="ACHUOY_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR0681311.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10592..11953
                     /gene="eutB"
                     /locus_tag="ACHUOY_000423"
                     /pseudo
     CDS             10592..11953
                     /gene="eutB"
                     /locus_tag="ACHUOY_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            11974..12861
                     /gene="eutC"
                     /locus_tag="ACHUOY_000424"
     CDS             11974..12861
                     /gene="eutC"
                     /locus_tag="ACHUOY_000424"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR0681312.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12871..13530
                     /gene="eutL"
                     /locus_tag="ACHUOY_000425"
     CDS             12871..13530
                     /gene="eutL"
                     /locus_tag="ACHUOY_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR0681313.1"
                     /translation="MPALDLIRPSVTAMRVIASVNSEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13543..14043
                     /gene="eutK"
                     /locus_tag="ACHUOY_000426"
     CDS             13543..14043
                     /gene="eutK"
                     /locus_tag="ACHUOY_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR0681314.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            14089..15141
                     /gene="eutR"
                     /locus_tag="ACHUOY_000427"
     CDS             14089..15141
                     /gene="eutR"
                     /locus_tag="ACHUOY_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR0681315.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(15147..16046)
                     /gene="hemF"
                     /locus_tag="ACHUOY_000428"
     CDS             complement(15147..16046)
                     /gene="hemF"
                     /locus_tag="ACHUOY_000428"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR0681316.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16050..16919)
                     /gene="amiA"
                     /locus_tag="ACHUOY_000429"
     CDS             complement(16050..16919)
                     /gene="amiA"
                     /locus_tag="ACHUOY_000429"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR0681317.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTVAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            17133..17558
                     /locus_tag="ACHUOY_000430"
     CDS             17133..17558
                     /locus_tag="ACHUOY_000430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR0681318.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17545..17994
                     /locus_tag="ACHUOY_000431"
     CDS             17545..17994
                     /locus_tag="ACHUOY_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR0681319.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            18055..18630
                     /locus_tag="ACHUOY_000432"
     CDS             18055..18630
                     /locus_tag="ACHUOY_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR0681320.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18726..19625
                     /gene="yfeX"
                     /locus_tag="ACHUOY_000433"
     CDS             18726..19625
                     /gene="yfeX"
                     /locus_tag="ACHUOY_000433"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR0681321.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19683..20987)
                     /gene="pbp4b"
                     /locus_tag="ACHUOY_000434"
     CDS             complement(19683..20987)
                     /gene="pbp4b"
                     /locus_tag="ACHUOY_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR0681322.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20992..22416)
                     /gene="murP"
                     /locus_tag="ACHUOY_000435"
                     /pseudo
     CDS             complement(20992..22416)
                     /gene="murP"
                     /locus_tag="ACHUOY_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(22420..23316)
                     /gene="murQ"
                     /locus_tag="ACHUOY_000436"
     CDS             complement(22420..23316)
                     /gene="murQ"
                     /locus_tag="ACHUOY_000436"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR0681323.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23480..24337
                     /gene="murR"
                     /locus_tag="ACHUOY_000437"
     CDS             23480..24337
                     /gene="murR"
                     /locus_tag="ACHUOY_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR0681324.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24466..25257
                     /gene="ucpA"
                     /locus_tag="ACHUOY_000438"
     CDS             24466..25257
                     /gene="ucpA"
                     /locus_tag="ACHUOY_000438"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR0681325.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25428..26444
                     /gene="cysP"
                     /locus_tag="ACHUOY_000439"
     CDS             25428..26444
                     /gene="cysP"
                     /locus_tag="ACHUOY_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR0681326.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26444..27277
                     /gene="cysT"
                     /locus_tag="ACHUOY_000440"
     CDS             26444..27277
                     /gene="cysT"
                     /locus_tag="ACHUOY_000440"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR0681327.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27277..28152
                     /gene="cysW"
                     /locus_tag="ACHUOY_000441"
     CDS             27277..28152
                     /gene="cysW"
                     /locus_tag="ACHUOY_000441"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR0681328.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            28142..29239
                     /gene="cysA"
                     /locus_tag="ACHUOY_000442"
     CDS             28142..29239
                     /gene="cysA"
                     /locus_tag="ACHUOY_000442"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR0681329.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29373..30284
                     /gene="cysM"
                     /locus_tag="ACHUOY_000443"
     CDS             29373..30284
                     /gene="cysM"
                     /locus_tag="ACHUOY_000443"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR0681330.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30287..30655)
                     /locus_tag="ACHUOY_000444"
     CDS             complement(30287..30655)
                     /locus_tag="ACHUOY_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR0681331.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30760..31611
                     /gene="pdxK"
                     /locus_tag="ACHUOY_000445"
                     /gene_synonym="thiJ"
     CDS             30760..31611
                     /gene="pdxK"
                     /locus_tag="ACHUOY_000445"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR0681332.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         7161 a         8171 c         9149 g         7146 t
ORIGIN      
        1 attcatgcgt tgcatatgaa agtttatgca ccacagcgaa tatctctcca ttcttagtga
       61 tctacctcac cttttaaacg cgcttgccga attttgttat ttactctgac gaaaaattgt
      121 cacgatacac gaaagttttt cacaggcggc gactcatgga taaagaacgc atcattcagg
      181 aatttgtgcc cggcaaacag gtcacactgg cgcatctcat cgcgcatccc ggtgaggaac
      241 tggcgaagaa aatcggcgtt ccggatgcgg gcgtaattgg cattatgacg ctaactcccg
      301 gcgaaacggc gatgatcgtc ggtgatttag cgctgaaagc cgctgatgtg catatcggtt
      361 ttctcgaccg cttcagcggt gcattggtga tttatggctc ggtgggcgca gtagaagaag
      421 cgttatcaca aaccgtcagc ggtttaggtc gcttattaaa ttatacgctt tgtgaaatga
      481 ccaaaagtta atacgaggca ttcatgaaac gaattgcgtt tgtgggtagc gtcggggcag
      541 gaaaaacaac gttatttaat gcgttgcagg ggaattatac cctcgccaga aaaacacagg
      601 ccgtggaatt taatgataag ggcgatattg atacgccggg tgaatatttt agccatcccc
      661 gctggtatca cgccttaatt accacgctac aggatgtaga catgctgatc tatgtccatg
      721 gcgcaaatga cccggaaagt cggttgcctg ccgggttgct ggatattggc gtcagcaaac
      781 ggcaaatcgc cgtcatcagt aaaacggaca tgccagatgc tgacgtcgcc gcaacgcgaa
      841 agttattgct tgagacgggt ttcgaagagc cgattttcga gctaaacagc cacgacccgc
      901 aaagcgtgca gcaactggtg gattatctgg catctctcac caaacaggag gaagcaggtg
      961 aaaaaactca tcacagcgaa tgatattcgt gaagcacacg cgcgcggcga acaggcaatg
     1021 tccgtggttc tgcgcgccag cattattacc ccggaagccc gtaaagtggc ggatctactg
     1081 ggttttacca tcaccgaatg tgatgaatcg atcccggtaa cggcgtccgt gcccgccagc
     1141 gtgccagccg acaaaaccga aagccagcgc atccgcgaaa ccatcattgc ccagctgccg
     1201 gaaggccagt ttaccgaaag cctggtcgca cagctgatgg aaaaagtgat gaaggaaaaa
     1261 cagtcgctgg agcagggcgc aatgcagccg agcttcaaat cggtgaccgg caaaggcggc
     1321 atcaaagtga ttgatggcag cagcgtcaag tttggtcgtt ttgatggcgc acaaccgcat
     1381 tgcgtgggct tgactgatct tgtgaccgga gacgacggca gcagcatggc cgccggtttt
     1441 atgcagtggg agaacgcatt cttcccgtgg accctgaact acgacgaaat cgacatggtg
     1501 ctggaagggg agctccatgt tcgccacgaa ggcgaaacca tgatcgccaa agcaggcgac
     1561 gtgatgttta tcccgaaagg ctccagcatc gaatttggta cgacatccag cgtgaaattc
     1621 ctgtatgtcg cctggccggc taactggcaa tccctatgaa agatttcatc accgaagcat
     1681 ggctaagagc gaaccatacg ctcagcgaag gcgcagagat ccatctccct gcggacagtc
     1741 gcctgacgcc ctctgcccgg gagttgctgg aaagccgcca tctgcgcatc aagtttattg
     1801 acgagcaggg ccgcctgttt gttgacgatg aacagcagca gccgcagccc gttcatgggc
     1861 tgaccagtag cgatgaacat ccgcaggcgt gctgcgaact gtgccgccag ccggtggcaa
     1921 aaaagccgga tacgctgact cacctgtcgg cggaaaaaat ggtcgccaaa agcgatccgc
     1981 gtctgggttt tcgcgcggtt ctcgacagca ccattgcgct ggcagtgtgg ctgcaaattg
     2041 aactggcgga accgtggcag ccgtggctgg cggatatccg ttcgcgtctt ggcaacatta
     2101 tgcgcgccga tgcgctgggg gaaccgctgg gctgccaggc gattgtcggg ctttctgacg
     2161 aagatctgca tcgactttct caccagccgc tgcgctatct cgaccacgat catctggtgc
     2221 cggaagccag ccacgggcgc gatgcggcgt tgctcaatct gctacgtacc aaagtgcggg
     2281 aaaccgaaac cgtagcggcg caggtgttta tcacccgcag ctttgaagta ttacgcccgg
     2341 acatcttgca ggcgctgaac cgcctttcga gcacggtcta cgtaatgatg atcctgagcg
     2401 taacgaagcc gccgctaacg gtgaagcaga tccaacaacg actgggggaa acgcaatgat
     2461 tattgaacgt tgtcgtgaac tggcgttgcg agcgcccgcc agagtggttt ttccggatgc
     2521 gttagatcaa cgtgtgctga aagctgcgca atatttacat caacaaggtc tggcaacgcc
     2581 cattctggtc gctaatccgt ttgaacttcg tcagtttgcg ctcagtcacg gcgtggcgat
     2641 ggacgggcta caggtgattg atccgcatgg caacctcgca atgcgggaag aatttgctca
     2701 tcgctggctg gcccgcgcgg gcgaaaaaac gccgccggat gcgctggaaa aacttaccga
     2761 tccgctgatg ttcgccgcag caatggtcag cgccggtaaa gcggatgtct gtatcgcggg
     2821 caacctctct tccacggcga atgtgctgcg tgccggatta cgtattatcg gcttgcagcc
     2881 aggctgtaaa acgctctcat ccattttcct gatgctgcca cagtacagcg gtccggcgtt
     2941 gggctttgcc gattgcagcg tggtgccaca gccgacggcg gcgcaactgg cggatatcgc
     3001 gcttgccagt gccgaaacct ggcgcgccct caccggagaa gagccgcgcg tggcgatgct
     3061 gtcgttttcc agtaacggta gcgcccgtca cccctgcgtt gccaacgtcc agcaggcgac
     3121 agaaatcgtc cgtgagcgcg caccaaagct ggtggttgat ggcgagttgc agtttgacgc
     3181 cgccttcgtg ccggaagtgg cggcgcaaaa agcgcctgcc agcccgctac agggcaaagc
     3241 caatgtgatg gtttttccgt cgctggaagc cggaaatatt ggctacaaaa tcgcacaacg
     3301 actcggcgga tatcgtgccg tcgggccatt gatacaagga cttgccgcgc cgatgcacga
     3361 tctctctcgt ggttgtagcg tgcaggaaat tatcgagctg gcgctggtgg cagctgtgcc
     3421 gcgtcagaca gaagtgaacc gcgaaagcag tttacaaaca ctggttgaat gaacggtccc
     3481 gttctggacc ctgataaaga ggaaagcaca atggaagcat taggaatgat cgaaacccgg
     3541 ggcctggttg cactgattga ggcttctgac gcgatggtta aagctgcgcg cgtcaagctg
     3601 gttggtgtta agcagattgg cggcggcctg tgtactgcca tggttcgtgg tgatgttgca
     3661 gcctgtaaag ccgcgaccga tgcaggtgcc gcagcggcac agcgtatcgg cgagctggta
     3721 tctgtacacg ttatcccgcg tccgcacggc gacctggaag aagtcttccc gatcggcctg
     3781 aaaggcgata gcagcaacct gtaatcaccc tgtgtgacgt cgattcgggc gtcacgctct
     3841 ctcctttttc attttacctt ctgcggaggg tagggggagc ttttacctga aatacgccac
     3901 ggaggcgggt atgaaactgg cagtcgtcac tggacaaatt gtttgtaccg tacgccatca
     3961 cggactggcg catgacaaat tgctgatggt ggaaatgatt gatccacaag gtaatcctga
     4021 cgggcaatgc gccgtcgcca tcgacaatat tggcgcggga accggggagt gggtgttact
     4081 ggtgagtggc agttccgccc gccaggcgca taaaagcgaa acgtcaccgg tcgatctgtg
     4141 cgtgattggc attgtcgatg aggtggtgtc tggcggtcag gtaattttcc acaaataagg
     4201 cagaacatca tgaatcaaca ggatattgaa caggtggtga aagcggtact gctgaaaatg
     4261 caaagcagtg acacgccgcc cgccgccgtt catgagatgg gcgttttcgc gtccctggat
     4321 gacgccgttg cggcagccaa agtcgcccag caagggttaa aaagcgtggc aatgcgccag
     4381 ttagccattg ctgccattcg tgaggcaggc gaaaaacacg ccagagattt agcggaactt
     4441 gccgtcagtg aaaccggcat gggccgcgtt gaagataaat ttgccaaaaa cgtcgctcag
     4501 gcgcgcggca cgccaggcgt tgagtgtctc tctccgcagg tgctgaccgg cgacaacggc
     4561 ctgactttaa ttgaaaacgc gccctggggc gtggtggctt cggtaactcc gtccactaac
     4621 ccggcggcaa ccgtaattaa taacgccatc agcctgattg ccgcgggcaa cagcgtcatt
     4681 tttgccccgc atccggcggc gaaaaaagtc tcccagcggg cgatcacgct gctcaaccag
     4741 gcgattgttg ccgcaggcgg gccggaaaac ttactggtta ctgtggcaaa tccggatatc
     4801 gaaaccgcgc agcgtttgtt caagtttccg ggcatcggcc ttctggtggt gaccggcggc
     4861 gaagcggtag tagaagcggc gcgtaaacac accaataaac gtctgattgc cgcaggcgct
     4921 ggcaacccgc cggtagtggt ggatgaaacc gccgacctcg cccgtgccgc tcagtccatc
     4981 gtcaaaggcg cttctttcga taacaacatc atttgtgccg acgaaaaggt actgattgtt
     5041 gttgatagcg tagccgatga actgatgcgt ctgatggaag gccagcacgc ggtgaaactg
     5101 accgcagaac aggcgcagca gctgcaaccg gtgttgctga aaaatatcga cgagcgcgga
     5161 aaaggcaccg tcagccgtga ctgggttggt cgcgatgcgg cgaaaatcgc ggcggcaatc
     5221 ggcctgaacg tgccgcaaga aacgcgtttg ctgtttgtgg aaaccactgc agaacatccg
     5281 tttgccgtga ccgaactgat gatgccggtg ctgcccgtcg tgcgcgtcgc caacgtggcg
     5341 gatgccattg cgctggcggt gaaactggaa ggcggttgcc accacacggc ggcaatgcac
     5401 tcgcgcaaca tcgaaaacat gaaccagatg gcgaatgcta ttgataccag cattttcgtt
     5461 aagaacggac cgcgcattgc cgggctgggg ctgggcgggg aaggctggac caccatgacc
     5521 atcaccacgc caaccggtga aggggtaact agcgtgcgta cgtttgtccg tctgcgtcgc
     5581 tgtgtattag tcgatgcgtt tcgcattgtt taaggagtaa cagatggcgc acgacgaaca
     5641 atggctcacc ccacgtctgc aaacggcggc aacgctgtgt aaccagacgc ccgccgcgac
     5701 ggaatctccg ctgtggctgg gggtggatct gggcacctgc gatgtggtgt cgatggttgt
     5761 cgaccgcgac ggtcagtcgg tggcggtatg cctcgactgg gccgatgtgg tccgcgacgg
     5821 catcgtctgg gatttcttcg gcgctgtcac cattgttcgt cgccatctcg acacgctcga
     5881 gcagcaattc ggtcgccgtt ttagccatgc ggcgacttca tttcctccag gtacggaccc
     5941 gcgtatttcc atcaacgtgc tggagtctgc tggactggaa gtgagccatg tgctggatga
     6001 gccaaccgca gttgcggatc tgctgcaact ggacaacgcc ggtgtagtgg atatcggcgg
     6061 cggcactacc ggcattgcca tcgtgaaaaa gggcaaggtg acgtactcgg cggatgaagc
     6121 aaccggcgga catcacatct ctctgactct cgccggaaat cgccgtattt cgctggaaga
     6181 ggcggagcag tacaagcgcg gtcacggtga agagatttgg cctacggtga aaccggtgta
     6241 cgaaaaaatg gcggacattg tcgcccggca tattgaaggg cagggcatta ctgatttatg
     6301 gctggcgggc ggctcttgta tgcaaccggg cgtggcggaa ctgtttcgca aacaattccc
     6361 ggcgttacag gtgcatttac cgcagcacag tttgtttatg accccgctgg cgatcgccag
     6421 tagtgggcga gagaaagcgg aggggctata tgcaaaatga attgcagacc gcgctctttc
     6481 aggcgttcga taccctgaat ctgcaacggg taaaaacatt tagcgttcca ccggtgacgc
     6541 tttgcggtcc gggctcggtg agcagttgcg gacagcaagc gcaaacgcgt gggctgaaac
     6601 atctgttcgt gatggcagac agctttttgc atcaggcagg gatgaccgcc gggctgacgc
     6661 gcagcctggc tgttaaaggc atcgccatga cgctctggcc atgtccggtg ggcgaaccgt
     6721 gcatcaccga cgtgtgtgca gccgtggcgc agttgcgtga gtcaggctgt gatggggtga
     6781 tcgcatttgg cggcggctcg gtgctggatg cggcgaaagc cgtggcgttg ctggtgacga
     6841 acccggatag cacgctggca gagatgtcag aaaccagcgt tctgcaaccg cgcttgccgc
     6901 tgattgccat tccaacgacc gccggaaccg gctctgaaac caccaatgta acggtgatta
     6961 tcgacgcggt gagcgggcgc aagcaggtgt tagcccatgc ctcgctgatg ccggatgtgg
     7021 cgattctcga cgccgcgttg accgaaggtg tgccgtcgca tgtcacggcg atgaccggca
     7081 ttgatgcgtt aacccatgcc attgaagcat acagcgccct gaacgctaca ccgtttaccg
     7141 acagtctggc gattggtgcc attgcgatga ttggcaaatc gctgccgaaa gcggtgggct
     7201 acggtcacga ccttgccgcg cgcgagagca tgttgctggc ttcatgtatg gcgggaatgg
     7261 cgttttccag tgcgggtctt gggttgtgcc acgcgatggc gcatcagccg ggcgcggcgc
     7321 tgcatattcc acacggtctg gcgaacgcca tgttgctgcc aacggtgatg gaatttaacc
     7381 ggatggtttg tcgtgaacgc tttagtcaga ttggtcgggc actgcgaact aaaaaatccg
     7441 acgatcgtga cgctattaac gcggtaagtg agctgattgc ggaagttggg attggtaaac
     7501 gactgggcga tgttggtgcg acatctgcgc attacggcgc atgggcgcag gccgcgctgg
     7561 aagatatttg tctgcgcagt aacccgcgta ccgccagcct ggaacagatt gtcggactgt
     7621 acgcggcggc gcaataaatg ccggatgcga cgcttgccgc gtcttatcca gcctacggga
     7681 ttgcacatgt agggcggata aggcgttcac gccgcatccg ccagtggcgc ggtgcaattg
     7741 ccggatgcga tgcttgccgc gtcttatccg gcctacggga ttgcacatgt agggcggata
     7801 aggcgttcac gccgcatccg ccagtggcgc ggtgcaattg ccggatgcga cgcttgccgc
     7861 gtctgatccg gccttcggga ttgcacatgt agggcggata aggcgtttac gccgcatccg
     7921 ccaataaata acaactaaca gggagtaaag gcgatgggaa ttaacgaaat catcatgtac
     7981 atcatgatgt tctttatgct gatagctgcc gtagacagga tcctgtcgca gttcggcggt
     8041 tctgctcgtt tcctcggtaa gttcggtaaa agtatcgaag gatcaggcgg tcagttcgaa
     8101 gaaggcttta tggcaatggg cgctctgggt ctggcgatgg tcggtatgac cgcgctggca
     8161 ccggtactgg ctcacgtact cgggccggta attatcccgg tttacgaaat gctcggcgca
     8221 aacccgtcaa tgttcgccgg aacgctgctg gcgtgcgata tgggcggctt cttcctcgcc
     8281 aaagagctgg cgggcggcga cgtagcagcg tggctatact ctgggttaat tcttgggtcg
     8341 atgatggggc caacgattgt gttttccatt ccggtggcgc tcggcattat cgaaccttct
     8401 gaccgtcgtt atctggcgct cggcgtgctg gcgggcattg tgaccattcc gattggctgt
     8461 attgctggtg gtctggttgc tatgtactcc ggtgtgcaga tcaacggtca gccggtggaa
     8521 ttcactttcg ccctgatcct gatgaacatg atcccggtga tcattgttgc gattctggtg
     8581 gcgctggggc tgaaattcat cccggaaaaa atgatcaacg gcttccagat cttcgccaaa
     8641 ttcctcgttg cattgatcac cctcggtctt gctgccgcgg tagttaagtt tctgcttggc
     8701 tgggaactga tccccggtct ggaccctatc tttatggccc ctggcgataa acccggtgaa
     8761 gtgatgcgcg ccattgaagt tatcggttct atctcctgcg ttctgttagg ggcgtatcca
     8821 atggtgctgc tgctgacccg ctggtttgaa aaaccgctga tgagcgtcgg taaagtgctg
     8881 aatatgaaca acatcgcggc agccggcatg gtggcaacgc ttgccaacaa catcccgatg
     8941 ttcggcatga tgaagcagat ggatacccgc ggcaaagtca tcaactgcgc cttcgccgtt
     9001 tccgctgctt tcgccctggg cgaccactta ggcttcgccg ctgccaacat gaacgccatg
     9061 atcttcccga tgattgtcgg caagttgatc ggcggcgtaa cggcgattgg cgtggcgatg
     9121 atgctggtgc caaaagaaga cgcgaccacg gctaaaaccg aagcggaggc acaatcgtga
     9181 acactcgcca gctattgagc gtcggtatcg atatcggcac caccaccacc caggtgattt
     9241 tctcccacct ggagctggtt aaccgtgcgg cggtgtcgca ggtgccgcgc tacgaattca
     9301 ttaaacgcga aattagctgg caaagcccgg tgttctttac ccctgtcgat aaacagggtg
     9361 gtttaaaaga agcggaactg aaaaccttaa tcctcgagca atatcaggct gcgggcattg
     9421 cgccggaaag cgttgattct ggtgccatca tcatcaccgg tgaaagcgcg aaaacccgca
     9481 atgctcgtcc ggcggtgatg gtgctctccc agtcgctggg cgattttgtc gttgccagcg
     9541 ccgggccgca cctcgaatcc gtcattgccg gtcacggtgc cggggcacaa accctttctg
     9601 aacaacggct gtgtcgggta ctgaatattg acatcggcgg tggcactgcg aactacgccc
     9661 tgttcgatgc cggaaaaatc agcggcactg cctgcctcaa cgtcggtggt cgcctgctgg
     9721 aaaccgacag ccaggggcgc gtggtttacg ctcataaacc ggggcagatg attgtggatg
     9781 agtgcttcgg cgcaggcacc gacgcccgtt cgctgaccgg cgcgcagctg gtgcaggtta
     9841 cccggcggat ggcagaactg attgtcgaag tgattgacgg aacgctctcg ccgctcgcgc
     9901 aggcattgat gcaaaccggt ttgctgcccg caggtgttac gcccgaaatc attacgcttt
     9961 ctggcggcgt gggcgaatgt tatcgccacc agcccgccga cccgttctgt tttgccgata
    10021 ttggcccgct gctggcaacg gcgctgcatg accatccgcg cctgcgtgag atgaacgtac
    10081 agtttccggc gcaaaccgtg cgcgccacgg tgattggcgc gggcgcgcat accctttcgc
    10141 tctctggcag cacaatctgg cttgagggcg tacaactgcc gctgcgcaat ttgccggtgg
    10201 cgatcccgat tgatgaaacg gatctggtga gtgcctggca acaggcgctg cttcagctgg
    10261 atcttgatcc caaaactgac gcgtacgtgc tggcgcttcc cgcctcgctg cctgtgcgtt
    10321 acgccgcggt actgacggtc atcaacgcgc tggtcgattt cgtcgcgcgt tttccaaatc
    10381 cgcatcccct gctggtggtg gccgggcagg actttggtaa agctctgggc atgttgttgc
    10441 gcccacagct acaacaactc ccgttggcag tcattgacga agtgattgtc cgcgcggggg
    10501 actatatcga cattggtacg cctctttttg gcggatcggt tgtgccggtg acggtgaaat
    10561 cactcgcatt tccttcctga gggaacgact tatgaaacta aagaccacat tgttcggcaa
    10621 tgtatatcag tttaaggatg taaaagaggt gctggctaaa gccaacgaac tgcgttcggg
    10681 ggatgtgctg gcgggcgttg cagcggcaag ctcacaggag cgcgtggcgg caaagcaggt
    10741 gttgtcggaa atgaccgtag cggacatccg caataatccg gtgattgcct atgaagatga
    10801 ctgcgtgacg cggctgattc aggacgatgt taacgaaacg gcctacaacc agattaaaaa
    10861 caggagcatc agcgaactgc gtgagtatgt gctgagcgat gaaaccagcg tggacgacat
    10921 tgcctttacc cgcaaagggc tgacctcgga agtggtcgcg gcggtagcga agatttgctc
    10981 caacgcggac ctgatctacg gcgcgaagaa aatgccggta atcaaaaagg ccaataccac
    11041 catcggtatt ccgggcacct tcagcgcccg tttgcagcca aatgacaccc gtgacgacgt
    11101 gcaaagtatc gctgcacaaa tctacgaagg gctttccttc ggggtgggcg atgcggtgat
    11161 cggcgttaac ccggtaactg acgacgtgga aaacttaagc cgcgtgttgg acaccattta
    11221 cggcgtgatc gacaaattca acatcccaac tcagggctgc gtactggcgc acgtcaccac
    11281 ccagatcgaa gcgattcgtc gcggcgcgcc tggcggactg attttccaga gtatctgtgg
    11341 cagcgaaaaa gggctgaaag agtttggcgt ggaactggcg atgctcgacg aagcgcgcgc
    11401 agtgggtgct gagttcaacc gtatcgccgg ggaaaactgc ctctacttcg aaaccgggca
    11461 aggttcggcg ctgtctgctg gcgctaactt cggcgcagac taggtgacga tggaagcacg
    11521 taactatggg ctggcgcgtc attacgatcc gtttatcgtc aacaccgtgg tcggctttat
    11581 tgggccggag tatctctaca acgaccgcca gattatccgt gctggcttag aagatcactt
    11641 tatgggcaag ctgagcggca tctctatggg ctgtgactgc tgttacacca accacgctga
    11701 tgctgaccag aacctcaacg aaaacctgat gatcctgctc gccaccgcag gctgcaacta
    11761 catcatgggg atgccgctgg gcgatgacat catgctcaac tatcagacca ccgcattcca
    11821 cgacactgcc actgtgcgtc agttactcaa cctgcgcccg tcaccggagt ttgaacgctg
    11881 gctggaaagc atgggcatta tggcaaacgg tcgcctgacc aaacgggcgg gcgatccgtc
    11941 actgttcttc tgatgacgcg gagataacac atcatggatc aaaaacagat tgaagaaatt
    12001 gtacgcagcg tgatggcgtc aatgggacaa gcggcccccg cgccgtcaga agcaaagtgc
    12061 gccaccacta actgtgcggc accggtgacc tcggaaagct gcgcgctgga tttaggttct
    12121 gcggaagcaa aagcgtggat tggtgttgaa aacccgcatc gcgcagacgt attaacagaa
    12181 ctgcgccgca gcaccgtggc ccgcgtttgt accggtcgta ccggtccgcg tccacgtacg
    12241 caggcgctgc tgcgtttcct ggccgatcac tctcgttcga aagataccgt actgaaagaa
    12301 gtgccggaag agtgggtgaa agcgcagggc ttgctggaag tacgctccga aattagcgac
    12361 aaaaatctct acctgactcg cccggatatg ggccgccgcc tgtgtgcaga agctgttgaa
    12421 gcgctgaaag cacagtgtgt tgctaatccg gacgtacagg tcgttatttc tgatggcctg
    12481 tcaactgatg ccatcaccgt gaactacgaa gagatcctgc cgccgctgat ggcgggcctg
    12541 aaacaggccg ggctgaaagt cggcacgcca ttctttgttc gttatggtcg cgtgaagatt
    12601 gaagatcaga tcggtgagat cctcggcgcg aaagtggtga tcctgctggt gggggaacgt
    12661 ccggggttgg gtcagtcaga aagcctctcc tgttacgccg tttactcgcc gcgtatggcg
    12721 accaccgttg aggccgatcg cacctgcatc tctaacattc accagggcgg cacgccgccg
    12781 gttgaagccg cagccgtcat tgtggatttg gcgaaacgta tgctggagca gaaagcatcc
    12841 ggcatcaaca tgacccgata aggaggcatc atgccagctt tagatttgat tcgaccgtcg
    12901 gtaaccgcta tgcgggtgat tgcctccgtt aactctgaat ttgcgcgtga actaaaattg
    12961 ccgccgcata ttcgtagcct cgggctgatt tctgctgatt ccgatgacgt cacctacatt
    13021 gcggctgacg aagcgaccaa gcaggcgatg gtcgaagtgg tatatggccg ctcgctgtat
    13081 gcgggagcag cacacggacc gtcaccgact gccggtgaag tgctgattat gcttggtggg
    13141 ccaaacccgg cggaagtgcg tgctggtctg gatgcgatgg ttgcgcatat tgaaaatggc
    13201 gctgctttcc agtgggctaa tgacgcagaa aacaccgcat tcctggcaca tgtagtttcg
    13261 cgtaccggtt cttatctttc atcaaccgcc gggatcacgc ttggcgatcc gatggcgtat
    13321 ctggtggcac cgccgctgga agcgacctac ggcattgatg cagcgttgaa atctgccgac
    13381 gtacagctgg tgacctatgt cccgccaccg tctgaaacca actactcggc agcattttta
    13441 accggtagcc aggccgcgtg taaagcagcc tgtaacgcct ttaccgatgc agtgctggaa
    13501 atcgcgcgta atccaatcca gcgtgcgtaa cggaggttgc cgatgatcaa tgcactggga
    13561 ttgctggaag tggacggcat ggtcgccgcg atagatgcag cggatgccat gctcaaagca
    13621 gctaacgttc gtctgctcag tcacgaagtg cttgaccctg gtcgcttaac gctggtggtg
    13681 gaaggcgatc tggcggcgtg tcgtgcggcg ctggacgcag gttgtgctgc cgcgatgcgt
    13741 accgggcgtg tcatcagccg caaggagatc ggtcggccag acgatgacac ccagtggctg
    13801 gttactggct ttaaccgcca gccgaagcaa cccgtaaagg aacccgacgc gccagttatc
    13861 gtcgcggaat ctgctgacga gttgttggcg ctgttaacat cagtacgtca gggaatgacg
    13921 gcaggagaag tggctgccca ctttggctgg ccgctggaaa aagccagaaa tgcgctcgaa
    13981 cagctctttt ctgccgggac gttacgtaaa cgcagtagtc gttatcgtct caagccccat
    14041 taacctgtcg gaggtgccgg gtgtcctaca acacccggca ttaacatcat gaaaaagacc
    14101 cgtacagcca atttgcacca tctttatcat gaacccttac ccgaaaacct gaagctcacg
    14161 ccgaaggtcg aagtggataa tgttcatcaa cgacagacaa cggatgtcta tgaacatgct
    14221 ttgacgatta ccgcctggca gcagatttac gatcagctgc atccgggcaa gtttcatggt
    14281 gaatttacgg aaattctgct cgatgatatt caggtttttc gtgaatacac cggtctggcg
    14341 ctgcgtcagt cgtgcctggt ctggccgaac tcgttctggt ttggcattcc ggcgacgcgc
    14401 ggtgagcagg gatttatcgg ttcgcaatgt ctgggaagcg cagaaatagc gacgcgcccc
    14461 ggtggaaccg agtttgaatt aagtacgccg gacgattata cgatcctggg cgtagtgctg
    14521 tctgaagatg tcatcacccg tcaggctaac tttttgcaca atccggatcg ggtattacat
    14581 atgctgcgta gccagtcggc gctggaagtg aaagagcagc ataaagccgc gctgtggggc
    14641 tttgtccaac aggcgctggc gacgttttgc gagaacccgg aaaatctcca tcagcctgca
    14701 gtgcgaaaag tgctggggga taatttgcta atggcgatgg gggcgatgct ggaagacgcg
    14761 caaccaatgg tgacggcgga aagcatcagt catcagagtt accgtagatt actttcccgc
    14821 gcccgtgaat atgtgctgga aaatatgtct gagctggtga cggtgctgga cttgtgtaat
    14881 caactgcatg tcagtcgccg cacgctacaa aacgcgtttc acgctatttt aggcattggc
    14941 ccaaacgcgt ggctgaaacg cattcgcctg aacgccgtac gccgcgaact gataagcccg
    15001 tggtcgcaaa gcacaacggt aaaagacgcc gccatgcagt ggggattctg gcatctgggg
    15061 caatttgcca cggattacca gcagctgttt gccgagaagc cgtcgttgac gttgcatcag
    15121 cggatgcggg aatgggggtg agggagttac acccaatccc tgaccttaat aaactcactt
    15181 aacgccgctt ctgggctgcc atcttttggc tgataatcat actcccagcg taccagtggc
    15241 ggcattgaca tcaggatcga ctcggtgcgc ccgccagttt gcaggccaaa cagagtgccg
    15301 cgatcccaga ccagattgaa ctcgacataa cgaccgcgac ggtagagctg aaaattgcgc
    15361 tcgcgctcgc cgtaggccat cgctttacgt cgctctacaa ttggtaaata agcgtcggtg
    15421 tagcctttgc ctaccgcctg cataaaggca aaacagtggt cgaaatctgg cgtgttcaga
    15481 tcatcaaaga acagcccgcc aataccgcgc tgttcgttgc gatgtttgag gtagaagtaa
    15541 tcgtcgcacc actttttgta acggggataa acgtcttcac caaatggcag gcacaggtca
    15601 cgggcggtgc ggtgccagtg aatggcgtct tcttcaaaac catagaaagg ggttaaatcg
    15661 aagccgccgc caaaccacca gacgggctct gcacccggtt tttcggcaat aaaaaaacgc
    15721 acattcgcgt ggctggtggg aacatacggg ttatgcggat gcactaccag tgaaacgccc
    15781 atcgcctcga aactgcgccc ggcaagttcc gggcgatgag cggtggcgga agcaggcatc
    15841 gcctcgccgt ggacgtgaga aaagttgacg cctgcctgtt cgaaaacacc accattacgc
    15901 aacacccgac tacgcccgcc gccgccagct tcgcgctgcc aactatcttc gacaaattct
    15961 gcgccatcga cggcggtcag ctgctgacaa atcgtatcct gaaggttgag cagaaactgt
    16021 ttaacctggt gtgcgtcggg tttcataact tatcgctttt tcgaatgtgc tttctggttg
    16081 tcgaaccagt ggaaataact gatcacgcct tcagcaatcg ctgtggcgat tttctgacga
    16141 aacgccaccg tgcctaacag ccgctcttct tccgggttgg tgataaacga ggtttccacc
    16201 agcaccgaag gaaccgacgg cgatttcaac accacaaacg cggcttgttc ggtgttgcgg
    16261 ctgtgcagtt tatgcaccgg cttaatcttt ttcagaatat gcgagccaag cgtcaggctg
    16321 tttttaatag tatcggtttg caccagatca aacagcactt gctgcaatag gtgatccttg
    16381 tcagtcgcct ttttaccggc aacttcatcg gcgcggtttt cgcgttcaga caggtatttc
    16441 gccattgcgc tacttgcccc acggttagag agggcaaata ccgaagcacc ggcagctttc
    16501 gggttggtaa agccatcggc atgaattgac ataaacagat ctgcgccatg tttatgggcg
    16561 atttcaacgc gatcgtaaag tgggataaac gtatcgccag aacgcgttaa acgcgcatca
    16621 atcccatgat tacgcaaaat ggaacggacg tttttagcaa tcgccagcac cacatgtttt
    16681 tctttcgaac cgttgcgtcc gatcgctccg gtatcaattc cgccgtggcc tggatcgaga
    16741 acaacgacac gtttgccgcc agattttttg gctttcggct tgctgtgtcc gttgctggtt
    16801 tttaaaggtt cgtctttggc gatggcttgc gacattcctg acaacgtcag ggcagccaaa
    16861 ccggctttca gcacctggcg gcgcgaagtg agtgttttta gtggtttaaa agtgctcata
    16921 cggcctgagt tgtaataata aagttccaga tgttatatcg tatcgcgtaa tccgttacga
    16981 cggtttgatg ttgagaattg ttttactttt catttcaata gttgacaact ccccattatg
    17041 ccactttttc aaccgatttt cgtcagatat tggctaattc gaccgttcac gccataatca
    17101 gccttttaac ccgcaaaaag gtaacgaata ccatggagat acgcgtattt cgccaggaag
    17161 atttcgaaga ggtcatcacc ctttgggagc gttgcgactt gctgcgtccg tggaacgatc
    17221 cggaaatgga catcgagcgt aagatgaacc atgacgtcag tttgtttttg gtcgctgagg
    17281 taaacggtga agtggtcgga acggtgatgg gcggttatga cgggcatcgc gggtctgctt
    17341 attatcttgg cgtgcatcca gagtttcgtg ggcgtgggat tgccaatgcg ttgcttaatc
    17401 ggctggagaa aaagctgatt gctcgtggct gcccgaaaat tcagatcaat gtgccggaag
    17461 ataacgacat ggtgctcgga atgtatgaac gcctgggata tgaacacgcc gacgtgctga
    17521 gtctgggtaa gcgtttgatt gaagatgaag agtactgagt tccatcctgt ccattatgat
    17581 gcgcacggac gcctgcgttt acccttgctc ttctggcttg tgctattgct tcaggcgcga
    17641 acctgggtgc tgtttgtcat tgccggtgcg tcgcgcgagc agggtactgc gctgctaaat
    17701 ctgttttatc ccgatcacga taatttctgg ctggggctga ttccaggcat tccggcggtg
    17761 ctggcgtttt tgttgagcgg tcggcgggct acatttcctc gcacttggca tgtgctctat
    17821 tttctgttgc tgttggcgca ggtggtttta ctttgctggc aaccgtggct gtggctgaac
    17881 ggtgaatccg ttagcggtat cggtctggcg ctggtagtgg cggatattgt ggcgttaatc
    17941 tggctgctga ccaatcgacg tttacgcgct tgttttaatg aagaaaaaga ataacggcac
    18001 tttttggtga atttgcactc caagcaacgt tattgaataa ccaaaggaag tgacatgaaa
    18061 tcgctgcgtt taatgttatg cgctatgccg ttgatgctga ccggctgttc cacgatgtcg
    18121 tcagttaact ggtcggccgc taacccgtgg aactggtttg gatcgtccac caaagtgagc
    18181 gagcagggcg tgggtgaatt aacggcgtcc acaccactgc aagaacaagc cattgctgat
    18241 gcgcttgatg gcgattatcg cctgcgcagc ggaatgaaaa ccgcgaacgg caacgtggtg
    18301 cgcttttttg aagtgatgaa aggcgacaac gtggcgatgg tgattaacgg cgatcaaggc
    18361 acgatcagtc gcattgacgt gctggatagc gatattcctg ctgacaccgg tgttaaaatc
    18421 ggtacaccgt ttagcgacct ttacagcaaa gcatttggca attgccaaaa agctgatggt
    18481 gatgataatc gtgccgtcga atgtaaagcc gaaggcagtc aacatattag ctaccagttc
    18541 agcggggaat ggcgtggtcc tgaagggtta atgccttcgg acgataccct gaaaaactgg
    18601 aaagtcagta aaattatctg gcggcgttaa tttgcgtctg aacaaaccgc cgccgcgaaa
    18661 aaacacgtaa aatagcgctc aacaatgcca cggattgcgt ggcattctta ttttcaggag
    18721 gaacaatgtc tcaggttcag agtggcattt tgccagaaca ttgccgcgcg gcgatttgga
    18781 tcgaagccaa cgtgaaaggg gaagttgacg ccctgcgtgc ggccagtaaa acatttgccg
    18841 acaaactggc aacttttgaa gcgaaattcc cggacgcgca tcttggtgcg gtggttgcct
    18901 ttggtaacaa cacctggcgc gctctgagcg gcggcgttgg ggcagaagag ctgaaagatt
    18961 ttccgggcta cggtaaaggc cttgcgccga ccacccagtt cgatgtgttg atccacattc
    19021 tttctctgcg tcacgacgta aacttctctg tcgcccaggc ggcgatggaa gcctttggtg
    19081 actgcattga agtgaaagaa gagatccacg gcttccgttg ggtggaagag cgtgacctga
    19141 gcggctttgt tgacggtaca gaaaacccgg cgggtgaaga gacgcgtcgc gaagtggcgg
    19201 ttatcaaaga cggcgtggat gccggcggca gctatgtgtt tgtgcagcgt tgggagcaca
    19261 acctgaagca gctcaaccgg atgagcgttc acgatcagga gatgatgatc gggcgcacca
    19321 aagaggccaa cgaagagatt gacggcgacg aacgtccgga aacctctcac ctcacccgcg
    19381 ttgatctgaa agaagatggc aaagggctga agattgttcg ccagagcctg ccgtacggca
    19441 ccgccagtgg cactcacggt ctgtacttct gcgcctactg cgcgcgtctg cataacattg
    19501 agcagcaact gctgagcatg tttggcgata ccgatggtaa gcgtgatgcg atgttgcgtt
    19561 tcaccaaacc ggtaaccggc ggctattact tcgcgccgtc gctggacaag ttaatggcgt
    19621 tgtaagcgtt tctgattcga tgattaaagg ccagccagta acaaaactga ctggcctttt
    19681 tattactttt gcttcaacgc cgcatacacc tgatcaacta cccaaccata agttgcaatc
    19741 ggtaactgac cgctttcgaa catattggga tttttttgtg gatcggcaac tggcgaatgg
    19801 ggcttgttgc ttaacatcac aatcgccata tgattgaccg gatcgataac ggttactgtc
    19861 ccggtccagc cagtgtgacc gtaggtttgc gggcttgcca gcgtgccaaa cgtcggcgtc
    19921 atggtggcat taccattcac gcgccagccg aggccaaaag tggcatcttc cttagagctg
    19981 gtggtgaaca tcttcaccgt ttccgcactg aacagctgca catcgccata gcccccgccg
    20041 ttcagcatcg tttgcattaa caccgcaata tcgccggtat tggaaaacaa acctgcgtgc
    20101 ccggaaacgc cgcccatcga ataaaaggct ttttcatcgt gcacctgacc ccagagagtg
    20161 gaggtgcgga tattcgggaa atggatcacg ccatcgcggg tattgccgtt caattccgtg
    20221 gcggcaattt gttgcggttt aaagcctttc agcagcgggt taaataccgt atgtgtcagg
    20281 ccgagcgggc gataaatcga ctcttcaaca tagcggtcaa gcggctgacc ggtaaccgac
    20341 tcgacgataa atccaagcag catataatcg acatcgctgt agatatgttt gctgccgggc
    20401 tgatattcca gcggcgtgcg cttgatcatc tccagcgtct ggcctttatc ctgggaatat
    20461 aacgcgcccg cgaccgcttt attcgggtat tgcggatctg ccgggaaacc gccgctgtga
    20521 tgcagcaggt cagaaatccg cagggtgttt ttgcctttaa tggtgtcatt tggactatcg
    20581 gcaaaccccg gaatatattt cgcaatccga tcgtcaggat gcagtttgcc ttcgctcatc
    20641 agcttttgca gggcgaagtt cgtggcgtac attttggtgt ttgaggccag gtcatacagc
    20701 gtcccggtgg tggctttgac aggctgttcc atcaacacgc tgccatcgta ctttttcgcc
    20761 gctccccagg ctttgcgata cacaatctga ttatctttaa tgatcagcag gtttacgccg
    20821 ggataaccga catcaatttg ctggctaatc cagcgatcca tctggttaag ccgttcgacg
    20881 ttaaaccctg ctttctctgg cgagctttct gtcagaacag ggtattttgc ggcgctgacg
    20941 ctacaactaa cagccagcag agaaagatag agcattgtcc gtttcatgat atcagtccag
    21001 attgacgtta cggcagccaa agagcgtggt gaaaataaac ccgcaaaccc atgccaccag
    21061 aatcccgcca gcataaaccg ccatggccgg aaggatgcct tgtgcggaag tcatcagcgg
    21121 cagcgctacc agaccagacg gcccaaaggc gctgtttaag cccatcggca gaccccatca
    21181 ggctatcagg ccgataaaca aaccgcccgc cgcgccgcct aaacaggcgg taacaaacgg
    21241 cttcatgcgg ggcagggtga caccgtaaat cagcggttca ccaacgccca gcaggccggg
    21301 aataatcgcc ccgcgtacct gactgcgtag cgcactgtgc ggttgcgccc gccagtagag
    21361 tgccagcgcc gcgcccacct ggcccgcgcc tgccattgaa aggatgggaa ataagctgtt
    21421 gaatccctag ctgtccatta acgcgaggta aacaggaata aagccctgat gcacgccaaa
    21481 taccacggcg atcaggaaca gcccggctaa aaccgcacaa ccgaacggat tactgttcag
    21541 gtgcataaac agccatgaca taccttcgaa taaccagcca cccagcggca taatgatcag
    21601 gtacgcaagc gtagcggtga tcagcagggt gattaacgag gtcagcagca tgtcgagatc
    21661 gtccgacata aagcggcgca ccatgccttc aatgcgggcg caggcccacg cggcaatcag
    21721 cacgccgata atattgccgc gcggatcgat gggcagacca aagaaatcgt gaaaaccggc
    21781 gtagtcaccg gtggttgcag cggggttata accgagcaaa aagagtgcgg caataattgc
    21841 gccattgacg cccgtgccgc caaatgcctg ggcggcgtta tagcccacca gaatcaccag
    21901 gaaagtgaac aaacctttgc tgaacacctt cataaaattc agcgcgtcgg gtagcgtgcc
    21961 ctgagcatct gccggaacgt gcatcaccgt ggcaattaac gtcgctatcc ccagcagcag
    22021 accggcggca ataaaaccgg ggatcagcgg cgtgaagatg gtggcgaatt tggcgagaaa
    22081 ttgttgcacg ccggaggttt gtttggcttt taactgacgt ttgttctggg cggcgatttc
    22141 tgcggcatcc tgtaccggtg cctcgcccag cagctcgctc atcgcttttg cggcacgatg
    22201 cgctttacca ggcccaaaaa cgacctgcac ctggtcgctg gtcaaaatga cgcccttcac
    22261 gccttccagc gttttgatat tgggatcaac cagtgaactg tcatgtacac ccagacgcag
    22321 gtgcgtcata cagttaccac aactggcgat atttcccagt ccgccgacac gggtaagaat
    22381 ggtgttcaga agttcactgc tgatctcttt ggccatgggt tattccttgt ctaaaacctg
    22441 acgaataaag ccgccgtgtt gatccaggcg ttttttagct tctgcggcat cgagattttt
    22501 cagcaccatc acaatggccg ttttacagtt gcgctcgcag gcaattaacg ccgcttccgc
    22561 ttgttcggca ttacatccgg tggcgttttt aacgatattg acctggcgca catgcagttt
    22621 ttcgttggtg gcgaccacat cgaccatcag gttgccgaac actttgccgg atttaatcat
    22681 cagcccggtg gaaagcatat tgagcaccag tttctgcgct gtgcctgctt tcattcgcga
    22741 agaaccagtg accacttcgg cacctacaat cggtgtaatg gcaaactcgg cggtggtgga
    22801 aacggcactc cccggattac aggaaattcc cactgtgcgg caaccgagct ggcgggcgta
    22861 ttccagtccg gcaatcacat acggcgtacg accgctggcg gcaataccga ccaccacatc
    22921 ctgtgccgtt aaatttatat ttttcagatc gttaatgccg ccttctcggc tatcttccgt
    22981 gccttccacc gcgtgctgaa tggcatattc gccgccagca atcaaaccaa ccaccagacc
    23041 cggtttcacg ccgtaggtgg gcggacattc gctggcatcc agaatcccca gacgaccgga
    23101 tgttcccgca ccgagataaa tcagacgacc gccgccgctt acctgggcgt ggataacatc
    23161 gatcgccgcg gcgatatccg gcaggacgcg ctcaacggca agcggtacgg ttttatcttc
    23221 atcgttgata atccggcaca tttccagcgt cgatacgcgg tcaatttcag ccgaggcggt
    23281 gttcgagcct tcagtaatca tcttttcaag ttgcatcggt ggtgacctta ctatttacga
    23341 tcaaataatt gcagatgtat ggaatcatat atcccgaatt gacagactta aaggaatcat
    23401 tgagtctaca cttgcagagt gtgaagagcg tcataagaac ttaagcggaa tttttgctgc
    23461 cagcccggga gagtggaaga tgttgtacct gacgaaaata cgcaatgcgg aaagtgaatt
    23521 taccgagaac gagcaaaaaa ttgctgactt tctgcgggcc aacgtgagtg agctgaaatc
    23581 cgtctcttcg cgcaagatgg cgaaacagct cggcatcagt cagtcgagca ttgtgaaatt
    23641 tgcccagaag ctcggcgcgc aaggatttac tgaattgcgt atggcgttaa ttggcgaata
    23701 cagtgccagc cgggagaaaa cgaatgccac ggcgctgcac ctgcacagtt cgattaccag
    23761 tgatgattcg ctggaagtga ttgcccgcaa actgaatcgc gaaaaagagc tggcgctgga
    23821 gcaaacctgt gcgttgttcg actacgctcg gctgcaaaaa atcattgagg tcatcagtaa
    23881 agcaccgttt atccagataa cgggcctggg cgggtcggcg ctggtggggc gcgatctgtc
    23941 gttcaaactg atgaaaattg gctatcgcgt ggcctatgaa gccgacacac acgtacaagc
    24001 caccgtttcc caggcgttga aaaaaggcga tgtacaaatc gctatctctt acagcggcag
    24061 taaaaaagag attgtcttgt gcgcagaagc ggcacgaaag cagggggcaa cggtgattgc
    24121 cattacttct ctggctgact ctccgctgcg gcggctggcg catttcacgc tcgataccgt
    24181 ttccggtgaa accgaatggc gtagctcgtc gatgtctacc cgcacggcgc aaaattctgt
    24241 taccgattta ctgtttgttg gtcttgtgca actcaatgat gtggagtcat tgaaaatgat
    24301 tcagcgcagc agtgagctaa cacaacgcct gaaatgatgc ataaagcagc gactggattg
    24361 agattttcct gaattagtga gctgatccgc agcaatattt tgtttatcct gtattttcag
    24421 agggaatgga gtgtaacgct ctgtattaac aaggagagca ttaaaatggg taaactcacg
    24481 ggcaagacag cactgattac gggcgcattg cagggaattg gcgaaggaat tgccagaact
    24541 tttgcgcgtc atggcgcaaa cctaatcttg ctggatatct cccctgagat cgaaaagctg
    24601 gcggacgaac tgtgtggtcg tggtcatcgc tgtacggcgg ttgtcgccga tgtgcgtgac
    24661 ccggcgtcgg tagccgcagc tatcaaacgc gcgaaggaaa aagaagggcg cattgatatc
    24721 ctggtgaata acgcaggcgt ttgtcgtctg ggcagtttcc tcgatatgag cgatgacgat
    24781 cgcgatttcc atattgacat caatattaaa ggcgtatgga acgtcacgaa ggcggtgctg
    24841 ccggagatga ttgcccgcaa agatggtcgc attgtgatga tgtcttcagt cactggtgat
    24901 atggtggccg atcctggcga aacggcgtac gccttaacga aagcggcgat tgttggcctg
    24961 acaaaatcgc tggcggtgga gtacgcgcag tctggtattc gcgttaacgc catttgcccg
    25021 ggatacgtgc gcacaccaat ggcggaaagc attgcccgcc agtcgaaccc ggaagatcca
    25081 gagtcggtgc tgactgaaat ggcgaaagca atcccgctgc gtcgcctcgc cgatccgctg
    25141 gaagtcggcg aactggcggc cttcctcgca tcggatgaat ccagctattt aaccggtaca
    25201 cagaatgtga ttgatggcgg cagcacactg ccggagacgg ttagcgtcgg tatctgattc
    25261 acctctgttt cctccctgca tttgtgggga ggatttcgtt ttaaacccgg ttcttattcc
    25321 cttttcaact tccaaatcac caaacggtat ataaaaccgt tactcctttc acgtccgtta
    25381 taaatatgat ggctattaga aagtcattaa atttataagg gtgcgcaatg gccgttaact
    25441 tactgaaaaa gaactcactc gcgctggtcg cttctctgct gctggcgggc catgtacagg
    25501 caacggaact gctgaacagt tcttatgacg tctcccgcga gctgtttgcc gccctgaacc
    25561 cgccgtttga gcaacaatgg accaaagaaa acggcggcga caaactgaca ataaaacaat
    25621 ctcatgccgg gtcatcaaaa caggcgctgg caattttgca gggcttaaaa gcagacgttg
    25681 tcacttataa ccaggtgact gacgtacaaa tcctgcatga caaaggcaag ctgatcccgg
    25741 cggactggca aacgcgcctg ccaaataata gctcgccatt ctactccacc atgggcttcc
    25801 tggtgcgtaa gggcaacccg aagaatatcc acgactggaa cgacctggtg cgctccgatg
    25861 tgaagctgat tttcccgaac ccgaaaacgt cgggtaacgc gcgttacacc tatctggcgg
    25921 catggggcgc agcggataaa gctgacggtg gcgataaagc caaaaccgaa cagtttatga
    25981 cccagttcct gaaaaacgtt gaagtgttcg atactggcgg tcgtggcgcg accaccactt
    26041 ttgccgagcg cggcctgggc gatgtgctga tcagcttcga gtcggaagtg aacaacatcc
    26101 gtaaacagta tgaagcgcag ggttttgaag tggtgattcc gaaaaccaac attctggcgg
    26161 aattcccggt ggcgtgggtt gataaaaacg tgcaggccaa cggtacggaa aaagccgcca
    26221 aagcctacct taactggctc tacagcccgc aggcgcaaac catcatcacc gactattact
    26281 accgcgtgaa taacccggaa gtcatggata aactgaaaga caaattcccg cagaccgagc
    26341 tgttccgcgt ggaagacaaa tttggctcct ggccggaagt gatgaaaacc cacttcacca
    26401 gcggcggcga gttagacaag ctgttagcgg cggggcgtaa ttaatgtttg cagtttcatc
    26461 aagacgcgtg ctgccgggct ttaccttaag cctcggcacc agtctgctgt ttgtgtgcct
    26521 gattttgctg ctgccgctct ccgcgctggt gatgcagctg gctcagatga gctgggcgca
    26581 gtactgggag gtgatcacca acccgcaggt agtagcggcc tacaaagtaa cgctgctgtc
    26641 agcgtttgtg gcatcgattt ttaacggcgt ttttggcctg ctgatggcgt ggatcttaac
    26701 ccgctatcgc tttccgggcc gcacgctgct tgatgcgctg atggatttac cctttgcgct
    26761 gccaacggca gtcgccggtt taacgctagc ctcgctcttt tctgtaaacg gtttttacgg
    26821 tgaatggctg gcgaagtttg atatcaaagt cacctatacc tggctgggga ttgcggtggc
    26881 gatggccttt accagcattc cgtttgtggt acgtaccgtg cagccggtgc tggaagagtt
    26941 aggcccggaa tatgaagaag cggcagaaac gcttggcgca acgcgctggc agagtttctg
    27001 caaagtggtg ctgccggaac tttctccggc gctggtggcg ggcgtggcgc tgtcgtttac
    27061 ccgtagtctt ggtgaatttg gcgcggtgat ttttatcgcc ggaaatatcg cgtggaagac
    27121 ggaagtgacg tcgctgatga tttttgtgcg cttacaggag tttgattacc cggcagcgag
    27181 cgcgattgct tcggtgatcc tcgcggcatc tctgctgctg ctgttctcaa ttaacactct
    27241 gcaaagtcgc tttggtcggc gtgtggtagg tcattaatgg cggaagttac ccaattgaag
    27301 cgttatgacg cgcgcccgat taactggggc aaatggtttc tgattggcat cgggatgctg
    27361 gtatcggcgt tcatcctgct ggtgccgatg atttatatct tcgtacaggc gttcagcaaa
    27421 gggctgatgc cggttttaca gaatctggcc gatccggaca tgctgcacgc catctggctg
    27481 acggtgatga tcgcgctgat tgccgtaccg gtaaacctgg tgttcggcat tctgctggcc
    27541 tggctggtga cgcgctttaa cttccctgga cgccagttac tgctgacgct actggacatt
    27601 ccgtttgccg tatcgccggt ggttgccggt ctggtgtatc tgctgttcta cggctctaac
    27661 ggcccgctgg gcggctggct ggatgaacat aatctgcaaa ttatgttctc ctggcccgga
    27721 atggtgctgg tcaccatctt tgtgacgtgt ccgtttgtgg tgcgcgagct ggtgccggtg
    27781 atgttaagcc agggcagcca ggaagacgaa gcggcgattt tgcttggcgc gtctggctgg
    27841 cagatgtttc gccgcgtcac attgccgaac attcgctggg cgctgcttta tggcgtggtg
    27901 ttgaccaacg cccgcgcaat tggcgagttt ggcgcggtgt cggtggtttc cggctcgatt
    27961 cgcggcgaaa ccctgtcgct gccgttacag attgaattgc tggagcagga ctacaacacc
    28021 gtcggctcct ttaccgctgc ggcactgtta acgctgatgg cgattatcac cctgttttta
    28081 aaaagtatgt tgcagtggcg cctggagaat caggaaaaac gcgcacagca ggaggaacat
    28141 catgagcatt gagattgcca atattaagaa gtcgtttggt cgcacccagg tgctgaacga
    28201 tatctcactg gatattcctt caggtcagat ggtcgcgttg ctggggccgt ccggttccgg
    28261 gaaaaccacg ctgctgcgca ttatcgccgg gctggagcat caaaccagcg ggcatattcg
    28321 cttccacggc accgacgtca gccgcctgca cgcgcgcgat cgcaaggtcg gtttcgtgtt
    28381 ccagcattac gcgctgttcc gccatatgac ggtattcgac aatatcgctt ttggcctgac
    28441 ggtgctgccg cgtcgcgatc gcccgaatgc cgcagccatc aaagcgaaag tgacaaaatt
    28501 gctggagatg gtgcagcttg ctcatctggc ggatcgttat ccggcacagc tttccggcgg
    28561 ccagaaacag cgcgtggcgc tggcgcgcgc gctggctgta gaaccgcaaa ttctgctgct
    28621 tgatgaaccg tttggcgcgc tggatgcgca ggtgcgtaaa gagctgcgtc gctggctgcg
    28681 tcaactgcat gaagagctga aattcaccag cgtgttcgtg acccacgacc aggaagaagc
    28741 gacggaagta gctgatcgtg tagtggtgat gagccagggc aatattgaac aggctgatgc
    28801 gccggagcag gtatggcgcg aaccagcgac ccgttttgtg ctcgaattta tgggcgaagt
    28861 gaaccgcctg cagggaacca ttcgcggcgg gcagttccat gttggcgcac atcgctggcc
    28921 gctgggctac acacctgctt atcaggggcc ggtggatctc ttcctgcgcc cgtgggaagt
    28981 ggatatcagc cgccgtacca gtctcgattc gccgctgccg gtacaggtac tggaagccag
    29041 cccgaaaggt cactacaccc aattagtggt gcagccgctg gggtggtaca acgaaccgct
    29101 gacggtcgtg atgcatggcg acgatgcccc gcagcgtggc gagcgtttat tcgttggtct
    29161 gcaacatgcg cggctgtata acggcgacga gcgtatcgaa acccgcgatg aggaacttgc
    29221 tctcgcacaa agcgcctgat aggttgagtg aatgttaaac gcccggaggc gcttcccgcg
    29281 atccgggctt tttaatggca aggtttgtaa cctgtagacc tgataagacg cgcaagcgtc
    29341 gcatcaggca acaccacgta tggatagaga tcgtgagtac attagaacaa acaataggca
    29401 atacgcctct ggtgaagttg cagcgaatgg ggccggataa cggcagtgaa gtgtggttaa
    29461 aactggaagg caataacccg gcaggttcgg tgaaagatcg tgcggcactt tcgatgatcg
    29521 tcgaggcgga aaagcgcggg gaaattaaac cgggtgatgt cttaatcgaa gccaccagtg
    29581 gtaacaccgg cattgcgctg gcaatgattg ccgcgctgaa aggctatcgc atgaaattgc
    29641 tgatgcccga caacatgagc caggaacgcc gtgcggcgat gcgtgcttat ggtgcggaac
    29701 tgattcttgt caccaaagag cagggcatgg aaggtgcgcg cgatctggcg ctggagatgg
    29761 cgaatcgtgg cgaaggaaag ctgctcgatc agttcaataa tcccgataac ccttacgccc
    29821 attacaccac taccgggccg gaaatctggc agcaaaccgg cgggcgcatt acccattttg
    29881 tctccagcat gggaacaacc ggcactatca ccggcgtttc acgctttatg cgcgaacaat
    29941 ccaaaccagt aacgattgtt ggcctgcagc cggaagaagg cagcagtatt cccggcattc
    30001 gccgctggcc tgcggaatat ctgccgggga ttttcaacgc ttctctggtg gatgaggtgc
    30061 tggatattca tcagcgcgat gcagaaaaca ccatgcgcga actggcagtg cgggaaggaa
    30121 tattctgtgg cgtcagctcc ggcggcgcgg ttgccggagc actgcgggtg gcaaaagcta
    30181 accctggtgc ggtggtggta gcgatcatct gcgatcgtgg cgatcgctac ctttccaccg
    30241 gggtgtttgg ggaagagcat tttagtcagg gggcggggat ttaagattat tgcgcaggca
    30301 ccgttttatc cgtctgcgca attaaggcat gtaaaaaagg ctgtgcgttc tcatcgctct
    30361 taccggggat cttcacaata tacggcttcc cggtaatcga tgacgacgaa gcaaccttat
    30421 caataaactg ctcggcagtg tcaatgcggt tacgggtatt gccgagcttc agacgcagat
    30481 gagaaaccgc ttcatcgcag gtatgttcat caccgttgcg cacaaatatc aaatcctttt
    30541 tctgtgctaa tccctccagc atggcgttga tgcgggcttc ttcatgaaca gttaacttcg
    30601 cgtaagcggg gagtgtcatt agtagtgtaa tgaccagaca aatgattttc ttcattgaat
    30661 taccttatcc ttgttttgtg atagccgatt tgactgccag aatcaggtaa agtttcggtt
    30721 ggaatacgat gaggatcatc ggcaaggaga aaaaattaaa tgagtagttt gttgttgttt
    30781 aacgataaga gtagggcgct gcaggcggat atcgtcgccg tgcagtcgca ggtagtgtac
    30841 ggcagcgtag gcaacagcat tgcggtgcct gctataaaac agaacggctt gaatgtcttt
    30901 gccgtgccga cggtattgct gagcaatacg ccgcattatg acactttcta cggtggtgcg
    30961 attccggacg aatggtttag cggctatttg cgtgcgcttc aggagcgtga tgcgctgcgc
    31021 caacttcgtg ctgtaaccac gggctatatg ggaacggcat cgcaaatcaa aatccttgcc
    31081 gagtggctga ctgcgctacg caaagaccat cctgacctat tgatcatggt cgatccggtg
    31141 attggcgata ttgatagcgg aatttatgtc aaacctgacc ttcccgaggc gtatcgacaa
    31201 tatttactgc cgctggcgca aggaattacc cccaatatct ttgagctgga aatcctgacc
    31261 ggtaaaaatt gccgcgatct cgacagtgcc attgctgccg caaaaagtct gctttcagac
    31321 acattaaaat gggtggtgat taccagcgcc tccggtaatg aagaaaatca ggagatgcag
    31381 gtggtggtgg tcagtgccga cagcgtgaat gtcatttccc attcacgggt aaaaaccgac
    31441 ctgaaaggga ctggcgacct gttttgtgct cagcttatca gtggtttgct gaaagggaag
    31501 gcgttaaacg atgcggtgca ccgtgcgggg ttgcgcgtac tggaagtgat gcgctatacc
    31561 cagcagcatg agagcgatga attgattttg ccgccgctgg cggaagcata aaaaatggcg
    31621 ccgatgg
//
LOCUS       DAZOYL010000013        30870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZOYL010000013 DAZOYL010000000
VERSION     DAZOYL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.013"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..948
                     /gene="tuf"
                     /locus_tag="ACHUOY_000446"
     CDS             <1..948
                     /gene="tuf"
                     /locus_tag="ACHUOY_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR0681333.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGD
                     NIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            1178..1561
                     /gene="secE"
                     /locus_tag="ACHUOY_000447"
     CDS             1178..1561
                     /gene="secE"
                     /locus_tag="ACHUOY_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR0681334.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1563..2108
                     /gene="nusG"
                     /locus_tag="ACHUOY_000448"
     CDS             1563..2108
                     /gene="nusG"
                     /locus_tag="ACHUOY_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR0681335.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2267..2695
                     /gene="rplK"
                     /locus_tag="ACHUOY_000449"
     CDS             2267..2695
                     /gene="rplK"
                     /locus_tag="ACHUOY_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR0681336.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2699..3403
                     /gene="rplA"
                     /locus_tag="ACHUOY_000450"
     CDS             2699..3403
                     /gene="rplA"
                     /locus_tag="ACHUOY_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR0681337.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3816..4313
                     /gene="rplJ"
                     /locus_tag="ACHUOY_000451"
     CDS             3816..4313
                     /gene="rplJ"
                     /locus_tag="ACHUOY_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR0681338.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4380..4745
                     /gene="rplL"
                     /locus_tag="ACHUOY_000452"
     CDS             4380..4745
                     /gene="rplL"
                     /locus_tag="ACHUOY_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR0681339.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            5065..9093
                     /gene="rpoB"
                     /locus_tag="ACHUOY_000453"
     CDS             5065..9093
                     /gene="rpoB"
                     /locus_tag="ACHUOY_000453"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR0681340.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            9170..13393
                     /gene="rpoC"
                     /locus_tag="ACHUOY_000454"
     CDS             9170..13393
                     /gene="rpoC"
                     /locus_tag="ACHUOY_000454"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR0681341.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13635..14768)
                     /gene="thiH"
                     /locus_tag="ACHUOY_000455"
     CDS             complement(13635..14768)
                     /gene="thiH"
                     /locus_tag="ACHUOY_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR0681342.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14765..15535)
                     /gene="thiG"
                     /locus_tag="ACHUOY_000456"
     CDS             complement(14765..15535)
                     /gene="thiG"
                     /locus_tag="ACHUOY_000456"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR0681343.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15537..15737)
                     /gene="thiS"
                     /locus_tag="ACHUOY_000457"
     CDS             complement(15537..15737)
                     /gene="thiS"
                     /locus_tag="ACHUOY_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR0681344.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15721..16476)
                     /gene="thiF"
                     /locus_tag="ACHUOY_000458"
     CDS             complement(15721..16476)
                     /gene="thiF"
                     /locus_tag="ACHUOY_000458"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR0681345.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16469..17104)
                     /gene="thiE"
                     /locus_tag="ACHUOY_000459"
     CDS             complement(16469..17104)
                     /gene="thiE"
                     /locus_tag="ACHUOY_000459"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR0681346.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17104..18999)
                     /gene="thiC"
                     /locus_tag="ACHUOY_000460"
     CDS             complement(17104..18999)
                     /gene="thiC"
                     /locus_tag="ACHUOY_000460"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR0681347.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19080..19180)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19233..19709)
                     /gene="rsd"
                     /locus_tag="ACHUOY_000461"
     CDS             complement(19233..19709)
                     /gene="rsd"
                     /locus_tag="ACHUOY_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR0681348.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19804..20577
                     /gene="nudC"
                     /locus_tag="ACHUOY_000462"
     CDS             19804..20577
                     /gene="nudC"
                     /locus_tag="ACHUOY_000462"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR0681349.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20617..21681
                     /gene="hemE"
                     /locus_tag="ACHUOY_000463"
     CDS             20617..21681
                     /gene="hemE"
                     /locus_tag="ACHUOY_000463"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR0681350.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21691..22362
                     /gene="nfi"
                     /locus_tag="ACHUOY_000464"
     CDS             21691..22362
                     /gene="nfi"
                     /locus_tag="ACHUOY_000464"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR0681351.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22405..22995
                     /locus_tag="ACHUOY_000465"
     CDS             22405..22995
                     /locus_tag="ACHUOY_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR0681352.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23182..23454
                     /gene="hupA"
                     /locus_tag="ACHUOY_000466"
     CDS             23182..23454
                     /gene="hupA"
                     /locus_tag="ACHUOY_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR0681353.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23467..24162
                     /locus_tag="ACHUOY_000467"
     CDS             23467..24162
                     /locus_tag="ACHUOY_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR0681354.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24164..24589)
                     /gene="zraP"
                     /locus_tag="ACHUOY_000468"
     CDS             complement(24164..24589)
                     /gene="zraP"
                     /locus_tag="ACHUOY_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR0681355.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24827..26203
                     /gene="zraS"
                     /locus_tag="ACHUOY_000469"
     CDS             24827..26203
                     /gene="zraS"
                     /locus_tag="ACHUOY_000469"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR0681356.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            26200..27525
                     /gene="zraR"
                     /locus_tag="ACHUOY_000470"
     CDS             26200..27525
                     /gene="zraR"
                     /locus_tag="ACHUOY_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR0681357.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27522..28811)
                     /gene="purD"
                     /locus_tag="ACHUOY_000471"
     CDS             complement(27522..28811)
                     /gene="purD"
                     /locus_tag="ACHUOY_000471"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR0681358.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28823..30412)
                     /gene="purH"
                     /locus_tag="ACHUOY_000472"
     CDS             complement(28823..30412)
                     /gene="purH"
                     /locus_tag="ACHUOY_000472"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR0681359.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7226 a         8035 c         8518 g         7091 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      721 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      781 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      841 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      901 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      961 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
     1021 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
     1081 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
     1141 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
     1201 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggt
     1261 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
     1321 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1381 cgttgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1441 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1501 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1561 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1621 gccgcgtagc aacgtcgcta cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1681 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1741 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1801 ggcacctggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1861 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1921 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1981 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     2041 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     2101 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     2161 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     2221 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     2281 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     2341 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2401 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2461 ttctttcact ttcgttacca agactccgcc ggcagcagtt ctgctgaaaa aagcggctgg
     2521 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2581 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2641 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2701 ggctaaactg accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa ccaaacagta
     2761 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2821 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2881 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2941 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     3001 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     3061 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgc ggcctgatgc caaacccgaa
     3121 agtgggtact gtaacgccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     3181 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     3241 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     3301 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3361 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3421 tgattttgtc tacaatctta cccccacata taatgcttaa tgcagacgta tatccgagat
     3481 attcgggttg tggcaaggcg gcaactgagt gagtcgccag gagcatagct aactatgtga
     3541 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3601 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3661 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3721 tgtttctgct caccgtaatt aagacgctct ctccgcttgg aggagtgaag tgagttccag
     3781 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3841 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3901 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3961 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     4021 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     4081 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     4141 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     4201 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     4261 gtaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     4321 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4381 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4441 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4501 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4561 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4621 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4681 aagacgacgc agaagcactg aaaaaatccc tggaagaagc tggcgctgaa gttgaagtta
     4741 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgactttttg
     4801 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4861 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4921 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4981 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     5041 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     5101 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     5161 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     5221 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     5281 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     5341 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5401 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5461 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5521 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5581 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5641 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5701 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5761 cgtgataaca agctgcagat ggagctggtg ccggaacgcc tgcgtggtga aactgcatct
     5821 ttcgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5881 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5941 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     6001 gcgaacatgg agctgagcct ggatctgttg gctaagctga gccagtctgg tcacaagcgt
     6061 attgaaacgc tgttcaccaa cgacctggat cacggcccgt atatctctga aaccttacgt
     6121 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     6181 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     6241 gaccgttatg acctgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     6301 gaaatcgaag gttccggtat cctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6361 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6421 atccgttctg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6481 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggacatg
     6541 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6601 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6661 ctcggcccgg gtggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6721 actcattacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6781 aactctttgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6841 aaggtgaccg acggtgttgt aactgacgaa attcactacc tttctgctat cgaagaaggc
     6901 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6961 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgtgacca ggttgactac
     7021 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     7081 cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact
     7141 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     7201 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     7261 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     7321 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7381 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7441 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7501 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7561 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg ggccggaaga gatcaccgct
     7621 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7681 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7741 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7801 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7861 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7921 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtttgttc
     7981 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     8041 ccgcgcgatc gctggctgga gctgggcctg accgacgaag agaaacaaaa tcagctggaa
     8101 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     8161 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggtg
     8221 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     8281 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     8341 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8401 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8461 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8521 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8581 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8641 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8701 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8761 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8821 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8881 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcacc
     8941 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     9001 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     9061 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     9121 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     9181 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     9241 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     9301 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9361 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9421 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9481 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9541 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9601 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9661 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9721 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9781 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9841 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9901 actgccgcca gatctgcgtc cactggttcc gctggatggt ggtcgtttcg cgacttctga
     9961 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
    10021 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
    10081 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
    10141 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
    10201 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
    10261 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
    10321 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10381 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10441 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10501 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10561 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10621 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10681 ggacgttgtt ctgggtctgt actatatgac ccgtgactgt gttaacgcca aaggcgaagg
    10741 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10801 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgcaaacg gtgaattagt
    10861 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10921 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10981 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    11041 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggta tcgatgacat
    11101 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    11161 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    11221 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    11281 aaccgtgatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    11341 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11401 tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11461 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11521 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11581 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11641 ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11701 tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct
    11761 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11821 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11881 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11941 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    12001 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    12061 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    12121 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    12181 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    12241 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    12301 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12361 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12421 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12481 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc tggcgcgtat
    12541 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12601 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12661 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12721 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12781 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12841 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12901 gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12961 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    13021 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    13081 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    13141 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    13201 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    13261 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    13321 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13381 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13441 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13501 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13561 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13621 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13681 cataccggct gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg
    13741 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13801 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13861 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13921 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13981 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    14041 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    14101 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    14161 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    14221 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    14281 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    14341 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14401 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14461 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14521 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14581 cgctgcgccc gttgggccag tggttccaga tagccactgg cggctggcga taacagcgcc
    14641 atcatatcgt cgcgggtgag ttgcgaggca tttagcgccc gttctacgtc agcagccgtt
    14701 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag
    14761 gttttcatgc cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac
    14821 tgcggctgcc cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg
    14881 ccttcgccat gttgacggga tcgtccgcta cggcaatcgc cgtattcact aacaccgcgt
    14941 cggcccccat ttccagggcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa
    15001 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    15061 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    15121 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    15181 gcatttcggc ggctttcaga gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    15241 gaatctctaa ttttaaccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    15301 tggcttcttc cgctgttttc gccccggaag tatttggcag cagggtcaca cccgccgcga
    15361 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15421 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15481 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15541 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15601 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15661 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15721 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15781 caggctgcgc cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc
    15841 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac
    15901 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag
    15961 gcagcggtaa cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc
    16021 gaccgcgctg gcggtgataa gcggcgtgtt gagtaccacg caggcggcat taatctcctg
    16081 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt
    16141 taacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatatcgg gattcaactg
    16201 tgtcagtcgc tgatggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag
    16261 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    16321 gacgccagcc cccgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16381 cagcacctgg ctgtcgagca gtttttgctg cccgtccaga gcgatatcgt cgagcagaat
    16441 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16501 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16561 tgccgacacc cgttgctatc accgcaggcg cgcgcgccag actgataccg ccaatcgcca
    16621 cggtgggata atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg
    16681 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16741 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16801 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16861 catacgcctg atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16921 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca ttgcgccgat
    16981 ctttgatgcg tagctggagg gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    17041 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    17101 acatcacgct tcctccttac gcaggtagat ttctccgcct ctggcgcgga agttctccga
    17161 catatccgcc attcccactt caatggtttg cgcggcggcg taatcacgta cttcctggct
    17221 gattttcatc gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttacctga
    17281 ctcttgcggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    17341 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17401 cgcgcctgga tgccctttcg ccaggtcagc ggcgtggacg gcaatcttat aggtgataag
    17461 cccctgctta acatcttctt tattaggcag acccagatgc tcttttggcg ttacgtaaca
    17521 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17581 atagcccggc gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17641 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc
    17701 ttcaatcatc acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg
    17761 cagctcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17821 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17881 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17941 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    18001 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    18061 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    18121 tgtaccgatc ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    18181 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    18241 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    18301 caggaaattg cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18361 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18421 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18481 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18541 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18601 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18661 gcgcacggta agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc
    18721 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18781 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18841 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18901 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18961 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagtcaag
    19021 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    19081 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    19141 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    19201 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    19261 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    19321 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19381 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19441 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19501 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19561 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19621 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19681 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19741 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19801 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19861 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19921 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt agtacaacag
    19981 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    20041 caactggccg gacgaggcgt acaactggcg gagttttacc gatcgcataa atactgtggt
    20101 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg cagccattgc
    20161 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    20221 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    20281 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    20341 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20401 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20461 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20521 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20581 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20641 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20701 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20761 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20821 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20881 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20941 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    21001 cgtaccattc gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    21061 tggacgctgg cgacctacat ggtggaaggc ggcagcagca aagcgttcac cgtgatcaaa
    21121 aaaatgatgt atgccgatcc gcaggcgctg cacgctctac tcgataaact ggcgaaaagc
    21181 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    21241 acctggggcg gtgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    21301 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21361 accaaaggcg gcggacagtg gctggaagcg atggcagaaa ccggttgcga tgcgttgggc
    21421 ctcgactgga caacggatat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21481 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21541 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21601 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21661 tctgaacaat atcaccgcta aggagtgatt atggatctcg cgtcattacg cgctcaacaa
    21721 atcgaactgg cttcttctgt gatccgcgag gatcgactcg ataaagatcc accggatctg
    21781 atcgccggag tcgatgtcgg gtttgagcag ggcggagaag tgacgcgagc ggcgatggtg
    21841 ctgctgaaat atccctcgct tgagctggtg gagtataaag ttgcccgcat cgccaccacc
    21901 atgccttaca ttccaggttt tctttccttc cgcgaatatc ctgcgctgct ggcagcgtgg
    21961 gagatgctgt cgcaaaagcc ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct
    22021 cgccgtcttg gcgtcgctag ccattttggt ttgatggtgg atgtgccaac gatcggcgtg
    22081 gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc
    22141 ccactgatgg ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac
    22201 ccgttgttta tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa
    22261 cgctgcatga aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg
    22321 gaacgtccgg cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat
    22381 aatttccgta tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc
    22441 ctggaaagct ggcagcatgt cactttcatg gcttgcttat gcgaacgcat gtaccccaat
    22501 tacgccatgt tctgccagca aaccggtttt ggtgatgggc aaatttaccg tcgtattctc
    22561 gatctcatct gggaaacgct gaccgttaaa gacgcaaaag taaatttcga cagccaactg
    22621 gagaaatttg aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg
    22681 gcaatcgatg cctgcgtggc gttaagtgaa ctggtccatt cgcgtttgag tggtgaaacg
    22741 ctcgaacacg cggtggaagt gagtaagacc tccatcacga ccgttgcgat gctggaaatg
    22801 actcaggctg gtcgcgaaat gagcgatgaa gagcttaaag aaaacccagc tgtagagcaa
    22861 gaatgggaca ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc
    22921 gagctgataa aaggccttag ggcagacctg cgtgaggcgg gtgagagcaa tattggtata
    22981 atttttcagc aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc
    23041 ttccctttcg ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc
    23101 gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata
    23161 aacacattgt aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa
    23221 gcagaactgt ccaaaactca ggctaaagct gctctggagt ccactctggc tgcaattact
    23281 gagtctctga aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac
    23341 caccgcgctg agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca
    23401 gctaacgtac cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc
    23461 gtggcagtga acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga
    23521 tcattgatgc tggctggcgc tctgcttctc actgcctgta gtcataactc ttcacttcct
    23581 ccctttaccg ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac
    23641 agcggcgata atgtgcatct gcttgccgtg tttagcccgt ggcgcagtgg cgataccacg
    23701 acgcgagagt atcgctggca gggcgataac ctcacgctca tcaatatcaa tgtttacagc
    23761 aaaccgccgg tgaatattcg cgcgcgtttt gacgatcgcg gtgatctgag ctttatgcaa
    23821 cgtgaatccg atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat
    23881 cgtgctgatc agatccgcca gattagcgat gccttacgtc aggggagagt cgtgctgcgc
    23941 caggggcgct ggcatgcgat ggaacagacc gtgaccacct gcgaagggca aaccattaaa
    24001 cctgatttag attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt
    24061 gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc aattactgtt agtggcaaac
    24121 tctgatttct gtcgctggca acccaacgag aaaacgttct gatttaccag tggcccatac
    24181 ccatatgacc gccaccaccg cagccgccgt agcccatgcc cattccggct ccgcgcggaa
    24241 tacccgcttc agccatcgcg atatctcgtt tcacccgtaa ctcatctaac gactgacgca
    24301 aattctccat ctctttggcg accgcgttaa ttttgctgct atccggtggg ttcgcggcta
    24361 acagggcatt gtattcataa cgcttcgtca ccagttgctg ttgcagtgcg ctgctttgag
    24421 cgtaaaagtc attatggatt ttctgccacg ctgtctgttg ttcgctggtc aaaggcgcgg
    24481 cattttgctg ccacataccg tgtccgccgt gagcaaatgc agatgtcgat cccatcgcca
    24541 ttgctgaaag cgccatcatt accagggcaa ttttcgtgtt ccgtttcatg gttaatcctc
    24601 cagtggttgt cttacttcgg gtattgcatc ttccgtgcca acgatgaaac gctgatatga
    24661 cgggtaatct ggcatgataa acgagtaaaa atgactcgcc tgctgcgggt agcgagtcat
    24721 ttttactcat tgaaacttaa gcctttgtgt tacagcgcag ggtaagcgct gataaaagat
    24781 ggcatgattt ctgctgtcag aaagggatga gcaggcaaag aagaagatgc gttttatgca
    24841 acgttctaaa gactccttag ctaaatggtt aagcgcgatc ctccccgtgg tcattgttgg
    24901 gctggtaggg ttgtttgcgg tgacggtgat tcgcgattat gggcgcgaga ctgccgccgc
    24961 cagacaaacg ctgctggaaa aaggcagtgt acttattcgc gctcttgaat ccggctcgcg
    25021 cgtcggcatg gggatgcgca tgcatcatgc gcagcagcag gccttactgg aagaaatggc
    25081 cgggcagcct ggagtacgtt ggtttgcggt cacggatgaa caaggaacaa tcgtgatgca
    25141 tagcaactcc ggcatggtgg gaaaacagct ttattccccg caggaaatgc agcagttaca
    25201 tccgggagat gaagaagcgt ggcggcggat cgatagcgca gacggcgagc ctgttctgga
    25261 aatttatcgc cagtttcaac cgatgtttgc tgctggaatg caccggatgc gccatatgca
    25321 gcagtatgcc gcgacaccac aagcaatttt catcgctttc gacgccagta atattgtgag
    25381 tgccgaagat cgtgagcaga gaaacaccct gattatcctc ttcgccctgg cgacggtctt
    25441 gctggcaagc gtattgtcat tcttctggta tcgccgctat ttgcgctcgc gccagcttct
    25501 acaagatgaa atgaagcgca aagagaagct ggtggcgctg gggcatcttg cggcaggcgt
    25561 tgcccacgaa atccgtaacc cactttcctc gattaaagga ctggcgaaat actttgccga
    25621 gcgcgcgcct gcagggggag aagcgcatca actggcgcag gtgatggcga aagaggccga
    25681 ccgtttaaac cgcgtggtaa gcgagctgct ggaactggtt aagccaacgc atctggcttt
    25741 gcaggcggtg gatctcaaca cgctgattaa ccactcatta cagctggtaa gccaggatgc
    25801 gaacagccgg gagatccagt tacgctttac cgccaacgac acattaccgg aaattcaggc
    25861 cgatccggac aggctgactc aggtcctgtt gaatctctat ctcaatgcta ttcaggcgat
    25921 tggtcagcat ggcgtgatta gcgtgacggc cagcgaaagc ggcgcgggcg tgaaaatcag
    25981 cgttaccgac agcggtaagg gaattgcggc agatcagctt gatgccatct tcactccgta
    26041 cttcaccact aaagccgaag gcaccggact ggggctggcg gtcgtgcata atattgttga
    26101 acaacacggt ggtacaattc aggtcgcaag ccaggaggga aaaggcgcaa cgttcaccct
    26161 ctggcttccg gtcactatta cgcgtaaggg cccacaagga tgacgcacga taatatcgat
    26221 attctggtgg tggatgatga cattagccac tgcactattt tgcaggcttt actgcgcggc
    26281 tggggctata acgtcgcgct ggcgaacagc gggcgacagg cgctggagca ggtgcgggaa
    26341 caggtttttg atcttgtgct ttgcgatgtg cgaatggcgg agatggacgg cattgccacg
    26401 ctaaaagaga tcaaagcgtt aaatccggcg attccggtgc tgattatgac cgcatactcc
    26461 agcgtcgaga cggcggtaga agcgttgaaa accggggcgc tggattatct catcaagccg
    26521 ctggatttcg ataacctgca ggcgacgctg gaaaaggcgc tcgcgcatac gcacagtatt
    26581 gacgctgaaa cgcctgcggt gtctgccagc cagttcggta tggtcggtaa aagcccggcg
    26641 atgcaacacc tgctcagtga aatcgccctc gtcgcgccat cggaagccac ggtgctgatc
    26701 cacggcgatt ccggcaccgg taaagagctg gtcgccaggg cgattcacgc cagtagcgca
    26761 cgtagcgaaa aaccgctggt aacgctcaac tgtgcggcac tcaacgaatc cttgctggaa
    26821 tctgaattgt tcggtcacga aaaaggggcg ttcactggag ccgatagacg ccgggaaggg
    26881 cgctttgttg aggcggacgg cggcacgctg tttctcgatg aaattggcga tatctcgccg
    26941 atgatgcagg tgcgtctgct gcgtgcgatt caggagcgcg aagttcagcg tgtcggtagc
    27001 aaccagacta tctcggttga tgtccggttg attgcggcga cccatcgcga tcttgccgca
    27061 gaggtgaatg ccgggcgttt tcgccaggat ctctactatc gcctgaatgt ggtggcgatt
    27121 gaagtaccat cgctgcggca acggcgggaa gatatccctc tgctggctgt ccattttctt
    27181 cagcgctttg ccgagcgtaa tcgcaaggcg gtaaaaggct ttacgccaca ggcgatggat
    27241 ttgctgatcc actacgactg gccgggaaat attcgtgaac tggaaaacgc ggtggaacgg
    27301 gcggtggtgc tgctgaccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc
    27361 ggtacgccga tcccactggg gcaaagtcag gatattcagc cgctggtgga agtcgaaaaa
    27421 gaagtgattc tggcggcact ggagaaaacg ggcggcaaca aaaccgaagc cgcccgtcag
    27481 ttagggatca cgcgcaaaac gctattggca aaactgtcgc gttagttctg ctcgcgttcg
    27541 atagcgcgcc agccgatatc tttccggcag aagcagtcgt cccagtgaat atcggtcatt
    27601 aaggcataag cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt
    27661 acgcgcccgc cgctggtcac tacctgctcg ttatccgcca gtttcgtgcc cgcgtggaac
    27721 actttgccgc ctgccacttc ttccagcggc aggccgtgga tcacatcgcc ggtgcggtaa
    27781 tcgcccggat atccacccgc agccatcacc acgccgagag aagcacgctc atcccactct
    27841 gacgttttct cgtccagttt gccttcacag gccgccaggc agagctcaac cagatcggac
    27901 ttcatgcgca gcataatcgg ctgggtttct ggatcgccaa agcggcagtt aaattcgata
    27961 accttcggat tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg
    28021 ttgccttccg ccgccatgcc tttcacggtt ggccagatga tacgttccat ggtgcgctga
    28081 tgaacttcat cggttactac cggcgcgggg gagtaagcgc ccatcccgcc cgtgtttggt
    28141 ccggtatctt tatcgcctac gcgtttgtga tcctggctgg tggccatcgg cagcacatgc
    28201 tcgccgtcta ccatcacgat aaagctcgct tcttcgccgt cgaggaactc ttcgataacg
    28261 atgcgatgac ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcg
    28321 gcttcttcca gcgtcatcgc cacgataacg cctttcccgg ctgccagacc gtccgcttta
    28381 atgacgatcg gcgcaccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag
    28441 ttctggtatt ccgctgtagg gatcttatgg cgggccagga aatctttggt aaacgctttc
    28501 gaaccttcca gttgcgcggc acctgcggtt gggccgaaga ttttcatccc ggcggcgcgg
    28561 aaggtatcga ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc
    28621 ttttcgtttt gtgcgaaatc caacagcgcc gggatatcgg tcacgccgat agcaacgttt
    28681 tgcagcgcgg gttccagcgc agtgcctgca ttacccggag caacaaaaac agtttcaacc
    28741 agcggcgact gcgccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact
    28801 aatactttca tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg
    28861 caataccgtg ctcgtcggcg gcggcaatca cttcgtcatc acggatagaa ccgccaggct
    28921 ggattacgca ggtcacgcct gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga
    28981 acgcgtcaga agccatcgag gaacctttca cttccaagcc ttcatcggca gctttaatac
    29041 cggcgatttt cgcggagtac acgcggctca tctggcccgc gccaatcccg atggtcatat
    29101 tgtttttggc atagacgata gcgttggatt tcacgaactt cgccactttc cagcagaaca
    29161 gcgcatcacg cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg
    29221 caccgaccat gcccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat
    29281 caagacccgg aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg
    29341 cggcggtgat tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact
    29401 gacgagaaat gatggcctgt gcggtttccg catccagctc gcggttaaag gcaatgatgc
    29461 cgccgaatgc ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat
    29521 tgccgatagc cacgccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga
    29581 actctttcac gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt
    29641 taccctgaac ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga
    29701 aggcagcctg ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca
    29761 ggttcagcgt gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg taagccggaa
    29821 ccatgctgcc gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt
    29881 tgatggcgag gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt
    29941 taataatggc gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag
    30001 cggcggagcg caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg
    30061 agcagccttc acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga
    30121 taggctggat ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc
    30181 caccatgtac tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg
    30241 tgtaatcgga aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc
    30301 ctgtagacag cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg
    30361 ctttgtcaga aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc
    30421 ccctggattt gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac
    30481 gcccctaacg gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc
    30541 tcgcgaattt ttctctttca atggtgatca caattttgac agtggttacc gtgggaaaaa
    30601 tacagaaatt acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt
    30661 tttgctgggg aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga
    30721 gaaatcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca cacaaacagc
    30781 cggttcggtt gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta
    30841 atatacgcca cctcgcgaca gtgcgctaaa
//
LOCUS       DAZOYL010000014        30105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZOYL010000014 DAZOYL010000000
VERSION     DAZOYL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.014"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            39..944
                     /gene="yijE"
                     /locus_tag="ACHUOY_000473"
     CDS             39..944
                     /gene="yijE"
                     /locus_tag="ACHUOY_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR0681360.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(971..1588)
                     /locus_tag="ACHUOY_000474"
     CDS             complement(971..1588)
                     /locus_tag="ACHUOY_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR0681361.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(1862..2965)
                     /gene="gldA"
                     /locus_tag="ACHUOY_000475"
     CDS             complement(1862..2965)
                     /gene="gldA"
                     /locus_tag="ACHUOY_000475"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR0681362.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(2976..3638)
                     /gene="fsa"
                     /locus_tag="ACHUOY_000476"
     CDS             complement(2976..3638)
                     /gene="fsa"
                     /locus_tag="ACHUOY_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0681363.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(3650..6151)
                     /gene="ptsP"
                     /locus_tag="ACHUOY_000477"
     CDS             complement(3650..6151)
                     /gene="ptsP"
                     /locus_tag="ACHUOY_000477"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0681364.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            6460..7539
                     /locus_tag="ACHUOY_000478"
     CDS             6460..7539
                     /locus_tag="ACHUOY_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR0681365.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTGGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            7554..7874
                     /locus_tag="ACHUOY_000479"
                     /pseudo
     CDS             7554..7874
                     /locus_tag="ACHUOY_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            7925..10221
                     /locus_tag="ACHUOY_000480"
                     /pseudo
     CDS             7925..10221
                     /locus_tag="ACHUOY_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            10187..11065
                     /locus_tag="ACHUOY_000481"
     CDS             10187..11065
                     /locus_tag="ACHUOY_000481"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR0681366.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            11067..11408
                     /locus_tag="ACHUOY_000482"
     CDS             11067..11408
                     /locus_tag="ACHUOY_000482"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0681367.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(11395..12246)
                     /locus_tag="ACHUOY_000483"
     CDS             complement(11395..12246)
                     /locus_tag="ACHUOY_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0681368.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(12461..14194)
                     /locus_tag="ACHUOY_000484"
     CDS             complement(12461..14194)
                     /locus_tag="ACHUOY_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR0681369.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(14377..17028)
                     /gene="ppc"
                     /locus_tag="ACHUOY_000485"
     CDS             complement(14377..17028)
                     /gene="ppc"
                     /locus_tag="ACHUOY_000485"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR0681370.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(17380..18531)
                     /gene="argE"
                     /locus_tag="ACHUOY_000486"
     CDS             complement(17380..18531)
                     /gene="argE"
                     /locus_tag="ACHUOY_000486"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR0681371.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            18685..19689
                     /gene="argC"
                     /locus_tag="ACHUOY_000487"
     CDS             18685..19689
                     /gene="argC"
                     /locus_tag="ACHUOY_000487"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR0681372.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            19697..20473
                     /gene="argB"
                     /locus_tag="ACHUOY_000488"
     CDS             19697..20473
                     /gene="argB"
                     /locus_tag="ACHUOY_000488"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR0681373.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            20534..21907
                     /gene="argH"
                     /locus_tag="ACHUOY_000489"
     CDS             20534..21907
                     /gene="argH"
                     /locus_tag="ACHUOY_000489"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR0681374.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            22174..23091
                     /gene="oxyR"
                     /locus_tag="ACHUOY_000490"
     CDS             22174..23091
                     /gene="oxyR"
                     /locus_tag="ACHUOY_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR0681375.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPIADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(23074..24474)
                     /gene="sthA"
                     /locus_tag="ACHUOY_000491"
     CDS             complement(23074..24474)
                     /gene="sthA"
                     /locus_tag="ACHUOY_000491"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR0681376.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            24795..25445
                     /gene="fabR"
                     /locus_tag="ACHUOY_000492"
     CDS             24795..25445
                     /gene="fabR"
                     /locus_tag="ACHUOY_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR0681377.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            25445..25804
                     /locus_tag="ACHUOY_000493"
     CDS             25445..25804
                     /locus_tag="ACHUOY_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR0681378.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(25844..26944)
                     /gene="trmA"
                     /locus_tag="ACHUOY_000494"
     CDS             complement(25844..26944)
                     /gene="trmA"
                     /locus_tag="ACHUOY_000494"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR0681379.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      27058..27248
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            27313..29157
                     /gene="btuB"
                     /locus_tag="ACHUOY_000495"
     CDS             27313..29157
                     /gene="btuB"
                     /locus_tag="ACHUOY_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR0681380.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            29102..29959
                     /gene="murI"
                     /locus_tag="ACHUOY_000496"
     CDS             29102..29959
                     /gene="murI"
                     /locus_tag="ACHUOY_000496"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR0681381.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6852 a         7565 c         8293 g         7395 t
ORIGIN      
        1 gtatagtgtc cacaggaaaa ctacacactg gatctctcat gtctgccgca ggaaagagca
       61 acccactggc aatcagtggc ctggttgtgc tcacacttat ctggagttat agctggattt
      121 tcatgaagca agtcaccagc tacatcggtg ccttcgactt taccgcctta cgctgcattt
      181 tcggcgctct cgttttattc atcgtccttt tattacgtgg tcgcggaatg cgcccgacac
      241 cgtttaaata caccttagcc attgccctgt tacaaacctg cgggatggtt ggcctggcgc
      301 agtgggcgct ggtcagcggc ggagcgggga aggtggcgat cctaagctat accatgccgt
      361 tctgggtggt gattttcgcc gcgttgtttc tcggtgaacg cctgcgacgt gggcaatatt
      421 tcgcgatact gattgccgct ttcggcttat ttttggtgtt gcagccgtgg caactcgatt
      481 tctcttcgat gaaaagtgcc atgctggcaa tcctctccgg cgtcagttgg ggggcgagcg
      541 cgattgttgc taaacgtctg tatgcccgtc atccgcgcgt ggatttattg tcgttaacat
      601 cctggcagat gctctatgcg gcgctggtga tgagtgtggt cgctttactg gtgccgcaac
      661 gtgaaattga ctggcagccc accgtgttct gggcgctggc ctacagtgcg attctggcga
      721 cggcactggc gtggagctta tggttgtttg tattgaaaaa cttgcctgcc agtattgcca
      781 gcttaagcac actggccgtt cccgtttgcg gcgtactctt ttcctggtgg ctgctcggcg
      841 agaatccggg ggccgttgaa ggtagcggta ttgtgctgat tgtgatggca ctggcgctgg
      901 tgagccgtaa gaaaaaagaa gccgttagtg taaaaaggat ctgaattttt tcttcatgtg
      961 gggcgatcgc ttatttaaca aaataacgat aatgccccac catgcgccag ctaaacagca
     1021 catcttcttc ctgcgcacct gcgccaatgt tatgtatcac cagcggcgta ccgtcgcggg
     1081 cgaagccatc tgaaaccacc ccaatatgtg ccagcccgtt atctaatcgc caggagacaa
     1141 tatcgcccgc ttgatagtca ctggggttct tgctgatggg gcgtgtttta tcgtggtggg
     1201 taaaccaggt ttccagatta ggcacccgac ggtgatcgat gttgctgtcc gggcgcttta
     1261 actgccattt ttgcgggtac tcggcaaaat tcttcgccat atcttcgtgc accagtttct
     1321 gcaaatcgac cttctggctg cgcaatgcac ggatcaccac atcggaacat accccgcgtt
     1381 tttgcgggac atcaccgcca ggataggtaa gctgcatata cgccggatcg taaaatagcg
     1441 tgctgccgat ttgctgtctg gcaccgtctg cgatggcaag gttggtattg gcctggattt
     1501 gtaccacggt tggtggaacg gcgggagatt ttaaggagtg gctggtaaat gccgttagca
     1561 ggctgagcag cgccagtgaa gctttcatct ggttcgtccg tgaaaataag agtcacagat
     1621 tcgaccttcc gggataacga ttgttctgta gagaaaatgt ccagaagcgg ataaaaaact
     1681 cctgaacaac gtgggagttt cagattactg gcacagagtg ccggatgcgg cgtgaacgcc
     1741 ttatctgtcc taaaaaaaaa gaaaattcaa tatattgcag agattgcgta ggcctgataa
     1801 gcgtagcgca tcaggcaatt ttgcgttcaa actcccggac aagccgggag tttggagtag
     1861 gttattccca ctcttgcagg aaacgctgac cgtactggtc ggctaccagc agagcggcgt
     1921 aaacctgatc tggcgtcgcg ccgccaggca tgttgtggat ggtttcacat tctgcacatg
     1981 ccgcttcagc cacaattcgc attttcgccg ggacatcttc tttaatatcc agttgagcga
     2041 gagttattgg caaacctacc gcatggctaa gtgcagctac ggtttcgatt tcctcaaccg
     2101 gtgcgttttc cagaaccagc tgcgtcagcg taccaaatgc cactttttca ccgtgataat
     2161 agtgatgcgc gtccgggata gcggtcaggc cgttatgcac tgcgtgcgcc gcagccagac
     2221 caccactttc aaaaccaaca ccgctcaaat aggtgttcgc ttcaatcacg cgctccagcg
     2281 ccggagtcac tacatgctgt tcggcagcaa gcatcgcttt ttcgccttct tccagcaggg
     2341 tgttgtagca cagttcagcc agtgccagcg cagcctgggt gcacttgccg cccgccatgg
     2401 tggtcgcgcc gctacgagag caggcacgcg cttcaaacca ggttgccagc gcatcgccga
     2461 tacccgccgc taacagacgt gcaggtgcgc cagcgacgat tttggtgtcg acaatgacca
     2521 tattcgggtt atttggcaac agcagatagc ggtcaaactc accctcatcg gtgtagataa
     2581 cagacaatgc gctgcacggt gcatcggtag aggcgatagt cggtgcgatc gctaccggaa
     2641 cacccatgaa atgtgccagt gctttggctg tatcgagggt ttttccgcca ccgataccga
     2701 gaattgcgcc acactgcgca gtctccgcga tgccacgcag acggtcgatc tcattttgcg
     2761 aacatccacc gccaaacggc gcaatttcta ctaccagtcc agcatcttta aagcttttct
     2821 cgacagtgga ttgagcaaaa cctaaaacaa atttgtcacc caccactaac cagcgttctg
     2881 ccagcggctt caggtattcg cccagacgat taatcacatc agcgccctgg atgtatttac
     2941 ccggtgattg aataatgcgg tccataattg ctcctttaga gatgagtagt gccaaatgcg
     3001 gcattccagt cgtgttcgaa cttctctata gctgactcta ccgcaggggt gttgagcatt
     3061 tgttgcgcta catctaaggg cagggtgatg gattcacatc ctgccagtaa acagtccagc
     3121 gcctgacgcg gcgttttaaa gctggctgcc agcaccatgc tttctggcgc gtgcatttct
     3181 aacagcgctt gcagctcctg aaccgtacga atgccgtctc cgccctgggc atctacgcgg
     3241 ttaacatacg gagcaacgta ttttgcccct gccagcgcgg ccagtaaccc ttgtgcagca
     3301 ctgtacactg cggtgccaag cgtagtaata ccctcttttt tcagtatttt aattgctgcc
     3361 agaccttcgg aagtcaccgg gattttcacc acaatacccg gaatagcgtc gcgcaggcgc
     3421 ttcgcttctt ccaccatccc ctgcgcgtcg cggctcatgg tctgagcaaa cagaatgccc
     3481 tcatcaccaa tcgctttttg cagacgcggc agcacttccc atatggactc cttgctggca
     3541 gcgataatgc tcgggttagt tgtcaccccg gcaatgggga atatgcgtgc cagacgttcg
     3601 acttctgcga cgttagcggt gtccagatac agttccatga tgtttccttt tacagttcca
     3661 gttcatgttg cagcaggctg gcgatagcgt cggcagaggc ggcgttaacc agcgcgttac
     3721 ggaattcttc gtgcatgatg cggcgagcga ggcgcgagaa aatgcgcatg tgctgatcgc
     3781 ccgcagcgtg tttgttcagg gttaacatga tgatgaattg cgcttcatca tcaccccagc
     3841 gcaccggcgc ttgcagacgc gcaacgctga tggtggattg ctcaatgtgt tctgatttgc
     3901 tgtgtggaat ggcaaaacta aagcccagac cggtagagaa aacggcctcg cgcgcccaca
     3961 agtcggcttc cagtttacgc ggatagcggc agcgccctgc cagcagcagg ttatcggtca
     4021 tgcctttgag cacttcttct ttgctgcgcc agtcgctttc cagtgtgatg cactcggcgg
     4081 tgaccagcgg tgcgtcttgt tgggtcatgc ggaattgcgc cagcaggtgc tccacttcca
     4141 gcgaagtacg gcaggccatt gcctggttga gcaacttgcg gcactcacgg ctatcaagtt
     4201 gcgccatccg cgctttcgcc gccggaattg atggtgcgct catgctgagt tcatccagcc
     4261 ctaagccgac cagcaacggc agcacggaac ctttcgctcc cagctcaccg cacagaccaa
     4321 tccatttacc ctggcgatgc accgcctgca cggcgtaatc gagcgcccgc aagaatgccg
     4381 gattcaggct gttgtagtga cgagtaacct tagcgttatc gcgatccact gccagcagat
     4441 attgcgtcag gtcattacta ccaatactaa agaaatcaat ctcttcgcag cattgatcga
     4501 tgatgaacat caccgacggc acttccagca taatgccgag ctggatcttc tcatcaaacg
     4561 gaatgtgttc gttacgtagt tgctgtttgg cttctgccag tttttctttc acccataaga
     4621 tctcttccat tgaggagatc atcgggatca tgattttcag gctgccgtga gcggaggcgc
     4681 gcaggatcga gcgtagttgt gtggtaaata acgacgcgta ttcttcataa atacgcacgg
     4741 cgcgataacc gaggaacggg tttgcctctg cgggaatgtt tagataatca acgggtttgt
     4801 cgccgccaat gtccatagtg cgcacaataa tgctgcgtcc gttggcagac tccagcgcct
     4861 gacaaaaaat gttgtacagc tcgctttcgc ccggtgcact ggtgcgatcc atatagagca
     4921 tttcagtgcg gaacaaacca acgccttccg caccattgcc gaatgcggcc tgcgcttcca
     4981 cggagtgagc gatgttagcg gcaatttcaa tgcggatacc gtcagcggta cgggcttgtt
     5041 gggtcagcca gacacgctgt tgctcacgca gggcgtcctg tacgcgggct tcttgctgat
     5101 aataacgagc tacggcttct cctggctcaa ccacaatcgc cccggcgttg ccgtcgatat
     5161 aaatcgtttg atgctgccac ggagtgaggg catcaatatc cacaccaacc agcgtcggaa
     5221 tgttgaacga acgggcaagg atcaccgtat gtgaggtggt gccgccactt ttcagcaaca
     5281 gacctttgag gtgattttta tccagttcga ggaactggct gggggtcagt tcatcagcca
     5341 tacaaatggc ggactgcgtc agtttgcccg gtgccgggaa gcgttgctca ccgtagattt
     5401 gctggagtaa ctggaagcag acgtcgcgta cgtccagggc acgttcttgc aggtagctgc
     5461 tgctggaacg ggcgaactct tcgcaaaagt gattcgcact ggcgacaatc gcttcggcgc
     5521 agcttaatcc ctcgctgacg cctgccagta agtgctgacg taaagatgta tcgccagcca
     5581 gggatcggtg agcttccagt atcgcgctgg tagcaccgtc gctatccagc agacgaaact
     5641 caatgttttt cagtaccagc gtcaggccgt tttccagtgt ggattgctcg gcgtcaacgt
     5701 ctttggctgc gggaagatta ctcagcgcat tgagatctaa agaagagatc ggcgtcagaa
     5761 tgccgcctgc actaccgctg cacacggtgc gggcgcggat aatttgcgga ttcagattgg
     5821 tcagtgaaac cggcagtggt tccagttcgt cagatttaac ttccgccagc ggcgcatcgc
     5881 agtgggggaa ttcatcgcgc agccattggc ttaaacgctg gtgcgcttcc tgttcgtcgg
     5941 ccccggaaat cagtaactgg cagttatcgc ccgccagcgt atcggtgcca atcagcgcaa
     6001 gggcgctttt ggcgttgccc ttgcggtcag tgtgcaggtt atgccactca atttgtgatg
     6061 aaaaagtatt acacagcgtt tcaacgtggc ttgccggacg cgcatgtacg ccgttaggta
     6121 gctcacaaat aaattccaca atcagggcca ttgcctctct cccggtacgg ttttctgttt
     6181 acaggataag agcgcacggc aacggcctgc catgtgacaa atctgccaaa agctggacaa
     6241 atgtaatgta accgtcaatt tgcgacgcgt ctcacaagac gccgttttgc ggcatgcttc
     6301 cggtttatcg caagttatga ggcggatcgc atttttgtac tgatattaca aaagtccagt
     6361 aaatggcctt tttatccact gtttgctccg cctgcgattg cctattgttt cctccatcaa
     6421 aaatatggac atgggctttc gcaagcccgg gagcagctta tgaatgagtt ggtgcagatc
     6481 ctgaaaaata cccgtcagca tttaatgacg ggcgtttcac acatgattcc cttcgtggta
     6541 tcgggcggta ttttgctggc ggtttccgtc atgttgtatg gcaaaggcgc agtgccggat
     6601 gccgtagccg atccaaatct gaaaaaactg tttgatatcg gcgttgcggg cttgacgctg
     6661 atggtgcctt tcctcgccgc ttacattggt tattccattg cagaacgttc tgcgctggct
     6721 ccatgcgcta tcggtacctg ggttggtaac agctttggtg cgggcttctt tggtgcactg
     6781 atcgccggga ttatcggcgg catcgtggtg cattacctga agaaaattcc ggtgcataaa
     6841 gttctgcgct cggtgatgcc tatcttcatt attcctatcg tcggcacact gattaccgca
     6901 ggcatcatga tgtggggctt gggcgagcct gtaggggcgt tgaccaacag cctgactcag
     6961 tggcttcagg ggatgcagca gggcagcatt gttatgctgg cggtgatcat gggtctgatg
     7021 ctggcgttcg atatgggcgg tccggttaac aaagtggcct atgccttcat gctgatttgc
     7081 gttgctcagg gtgtttatac cgtggtggcc atcgctgccg tgggtatttg tatcccgccg
     7141 ctggggatgg ggctggcgac gctgattggt cgtaaaaatt tctccgcaga agagcgcgaa
     7201 accggcaaag cggcactggt gatgggctgt gttggcgtta ctggaggggc gattcctttc
     7261 gccgctgccg atccgctgcg tgtcattcct tccatcatgg tcggttcagt ttgtggtgca
     7321 gtaactgcgg cgctggtcgg tgcgcagtgc tatgcaggct ggggtggtct gattgtgctg
     7381 ccggtggttg aaggcaagct gggttatatc gcagcagtgg ctgtcggagc agtggtgacg
     7441 gctgtttgtg tgaacgtgct gaaaagtctg gcgcgtaaaa atgggtcttc gactgatgaa
     7501 aaagaagacg acctggattt ggattttgaa attaattaat tgaggaacga gccatgacga
     7561 aaattattgc agtaaccgca tgtccttcag gtgttgccca tacctatatg gcagcagagg
     7621 cgctggaaag tgccgcgaaa gcgaaaggct gggaagtgaa agtagaaacc taggggtcaa
     7681 ttggtctgga aaatgaactg actgcggaag atgtggcgag cgctgatatg gtgattctga
     7741 ccaaagatat cggcatcaag tttgaagagc gttttgctgg caagaccatc gtgcgcgtca
     7801 acatcagcga tgcagtaaaa cgtgctgacg ccattatgag caaaattgaa gctcacctgg
     7861 cgcaaaccgc ttaagttctt ctcccgctcg caagggcggg ttcgctttcc cacaggagtt
     7921 cctcatgacg aatcgtatct ctcgcctcaa aactgcactg tttgccaata cccgtgaaat
     7981 ctcgctggag cgggcgctgc tttataccgc cagccatcgg caaaccgaag gcgaaccggt
     8041 gatattgcgc cgggcgaaag caacagcgta tatccttgaa catgttgaaa tttcgattcg
     8101 tgatgaagaa ctgattgccg gtaaccgcac cgtaaaaccg cgcgccggga ttatgtcgcc
     8161 ggaaatggac ccttactggc tgctgaaaga gctggatcaa ttcccgacgc gtccgcagga
     8221 ccgctttgct atcagcgaag aagataaacg tatctaccgt gaagagttgt tcccgtactg
     8281 ggaaaaacgt tcgatgaaag atttcatcaa cgggcagatg acagatgaag taaaagccgc
     8341 gaccaacacg cagattttca gcatcaacca gacggataaa ggccaggggc acattattat
     8401 tgattaccca cgcctgttga atcacggtct gggtgagctg gtggcacaga tgcagcaaca
     8461 ttgtcagcaa cagccggaga atcactttta tcaggccgcg ttgttactgc tggaagcctc
     8521 gcagaaacac attttgcgtt acgccgaact ggcggaaacg atggcggcaa actgcacaga
     8581 tgcacagcgt cgcgaagaac tgctgactat tgcggagatc tctcgccata acgccgaaca
     8641 taagccgcag acgttctggc aggcgtgcca gttattctgg tacatgaaca tcattctgca
     8701 atacgaatcc aacgccagtt cgctgtcttt agggcgcttc gaccagtata tgttgccgtt
     8761 ctatcaggca tctttaaccc agggcgaaga tccggcgttc ctgaaagaac tgctcgaatc
     8821 tttatgggtg aaatgcaacg acatcgtgct gttgcgctcc accagcagcg cgcgttattt
     8881 cgcaggtttc ccgaccggct ataccgcact gctcggcggg ttaaccgaga acggacgtag
     8941 cgcggtgaac gtgctttcgt tcctttgcct tgacgcctat caaagcgtgc aattaccgca
     9001 accgaacctc ggcgtgcgca ctaacgcctt gatcgacacg ccgttcctga tgaaaaccgc
     9061 cgaaaccatt cgcctcggca ccggtattcc gcaaatcttt aacgatgaag tggtggtacc
     9121 agcgttcctc aaccgtggcg tttcgctgga agatgcgcgc gactattccg tagtgggctg
     9181 tgtggaatta tctattcccg gcagaaccta cggcttgcat gacatcgcga tgtttaatct
     9241 gctgaaagtg atggaaatct gcctgcatga aaatgaaggc aatgctgcgc tgacttatga
     9301 aggtttactg gagcagatcc gcgccaagat cagccactac atcaccctga tggttgaggg
     9361 cagcaatatt tgtgatatcg gccatcgcga ctgggcacct gtaccgctgc tctcgtcttt
     9421 tatcagcgat tgtctggaaa aaggtcgcga tattaccgat ggcggcgcgc gttataactt
     9481 ctccggcgta caggggatcg gtatcgccaa cctgagcgat tctctccatg cgttgaaagg
     9541 gatggttttt gagcaacagc gtttaagttt tgacgaattg ctgtcggtat taaaagccaa
     9601 ctttgcaacg ccagaaggcg aaaaagtccg tgctcgctta attaaccgct tcgagaaata
     9661 cggtaacgat atcgacgagg tggataacat cagcgccgaa ctgttacgcc actactgcaa
     9721 agaagtggaa aaataccaga acccgcgcgc ggttacttca cgccgggatc gtataccgtt
     9781 tctgctcacg tcccgttggg atcggtggtt ggcgcgacgc cagacggtcg ttttgccggg
     9841 gaacaactgg cagacggcgg cttgtcacct atgctgggcc aggacgcaca agggccaaca
     9901 gcggtactga agtcagtcag taagctcgat aatacgctgc tgtctaacgg tacgttgctg
     9961 aacgtgaaat tcactccggc gaccctggaa ggtgaagcgg gattacgcaa actggccgac
    10021 ttcttacggg cgtttaccca gcttaagttg cagcatattc agtttaacgt ggtgaacgcc
    10081 gacacgttgc gggaagcgca acagcgccca caagattatg ccgggctggt ggtgcgcgtt
    10141 gccggataca gcgccttctt tgtcgaactg tcgaaggaga tccaggatga catcatccgc
    10201 cggacagcgc atcagctgta acgttgtgga aacgcgccgc gatgatgtgg cgcgcatttt
    10261 caacattcag cgttattcac tgaatgacgg cgagggcatt cgtacggtgg tcttttttaa
    10321 aggctgtccg catctttgcc cgtggtgtgc taatccggag tcgatctccg gcaaaatcca
    10381 gacggtacgc agagaggcga aatgtctgca ctgtgcgaaa tgtttgcgtg atgcggatga
    10441 atgcccctcc ggggcgtttg aacggattgg tcgcgatatc agccttgacg ctctggaacg
    10501 ggaagtgatg aaagatgaca ttttctttcg cacgtccggc ggcggcgtca cgctttctgg
    10561 cggcgaagtg ttaatgcagg cggagtttgc tacccgtttt ttacagcgac tgcggctgtg
    10621 gggtgtgtca tgcgccattg aaactgccgg agacgcgcca gcaagcaagc tgttaccgct
    10681 ggcgaaattg tgcgatgaag tgctgttcga tttaaaaatt atggacgcga ctcaggcgcg
    10741 ggatgtggtg aagatgaacc tgccacgcgt gctggagaat ctgcgtttgc tggtgagtga
    10801 gggtgtcaac gtgatcccgc gtttaccgtt gatccctggt ttcacgctca gcagggagaa
    10861 tatgcagcag gcgctggatg tactgatccc gctgaaaatc aggcagatcc atctgttacc
    10921 gtttcatcag tacggcgaac cgaaataccg cctgctgggg aaaacatggt cgatgaaaga
    10981 ggtgcctgcg ccgtcgtcag ccgatgtgac aacgatgcgc gaaatggcag aacgggccgg
    11041 atttcaggtt accgtgggag gttaaaatgg catacctggt ggcagtaacc gcctgcgtaa
    11101 gcggcgtggc gcatacttat atggcggcgg aacggctgga aaagttgtgc cagttagaga
    11161 agtggggcgt cagcattgaa actcagggcg cgctgggaac ggagaatcgt ttagcggacg
    11221 aggatatccg tcgggcggat gttgctctgt tgattacgga tatcgagctt gccggtgccg
    11281 agcgatttga acattgccgc tatgtgcaat gcagcatcta cgcattcctg cgtgagccgc
    11341 agcgggtaat gagcgcggtg cgcaaagtgc tttctgcgcc gcagcaaacc catcttattc
    11401 tggagtagtc ggtttttctg tcagttggct gtggtactgc cggcgatatt ccgacggcga
    11461 gcgttcggtg tttttacgaa acagacggca gaagtagttg ctgtcgacaa agccgcaggc
    11521 gtgcgccact tcttttacct tcaggtcata gcctttcaat aacgtcttag cgtgctccag
    11581 tcgcgtgtga ttcaggtatt cgttaaaacc aatggccccc gttttttgaa acaggtgcga
    11641 gagatagttt ggtgaaatat agaatgcctg tgcgacagat tcgcgggtaa gcgcggaggc
    11701 atagcgttcg tcgatataat cgcgaatagc ttcaaatagt gcctggctgc gtgaggcggt
    11761 ctggatttgg ctgccgagca aatcgcggca gtggctaagc aggctggcta cgataagcct
    11821 tgccgtttgc tgctcctgcg gctgcatttg catttcattg agcgtttgta gcagaaaaga
    11881 acctatacgt gggccgcgtc gggcgacgtg ttgcttcgcc agattttgat attgtttgcc
    11941 atcccattgc acgacgctga agccgagttg ctgtttgcca aacagcacgc taaaggtggt
    12001 agcgggtgtt ttccattgtg ggaaattcca gccaccagcg gcaacgtaca gcacatcgcc
    12061 gggaactaac gttgctccag cgccggtatc aacaaactca ccttccagca caatttccag
    12121 ccgtggaaaa tcgacctgat acgcaagatc gggcaccggg ccgttactgc tggcaaagta
    12181 aatctggcgc agggacagcg ggccgttgat caggcgggag agcagatagc tgacgccgtg
    12241 atacatgtta ttcccttagg tatgtagacg ggaaaacagt aactggcatt attaaaaaag
    12301 gccacctgaa aagtgtggcc tgaggggagt tcgatgtaaa cattgcggca gttgtattgc
    12361 cggatgtggc gtaaacgcct tatccagcct accgccgcga tctgtaggcc ggataagacg
    12421 cgtcaagcgt cgcatccggc aaatagcgcc tggctgataa ttactgatta cctacctggt
    12481 cgccatacgg cagagtgtcg taatcgatca gcgcgttttt cttgtacggg ttaccaatcc
    12541 agcgggtagt ttctttgaac tgcggattca ccactgaacg ggttggatcg taaccgtcgt
    12601 aagataagcc cgccaaatct gaccaggtgt ggatcagttc cgccaggctg tatttacgat
    12661 cgacatcctg tgagaaatca cggggatgag tcgcttgcca tttttccgac gtccacagca
    12721 ggaacggaat ggtgtacatg tggcgcgtcg ggttgtcttc gttacgcccc tgagttttgt
    12781 gcggcggcgt gtcgtaaacc tcttcaccgt ggtcagagaa gtaaaccagg aatccgttcg
    12841 ggtctgccgc tttaaagtct ttaatcaggc tggcaaccac atgatcgtta tacaggttag
    12901 cgttgtcata atcgttatat gattccagct cttctgcgct taatcctggc ggaacatgat
    12961 cggtattgcc atcaaacttg ccctgatttt ccgggtagcg gtatttgtat ttgatatgcg
    13021 tacccagcag atgaacgatg atcagtttct tcggcgcagg gtcattcagc acatcctgga
    13081 acggcttcag cacgttggtg tcgtattcac gcgcactctg cgtacgttgc tggttcatgt
    13141 agtactgctt gtcggtctgg cgcgaaaata ccgtcagcat ggtattgcgg gcggtcatcg
    13201 tctgctggtt ggtgatccag aaggttttat aacccgcctg tttcatcatg ttcatcagcg
    13261 acggctgcgt cagatacaga tccgggttct tttcattggc aaaggtcagc gcctgttgca
    13321 ggatttcaat ggtgtacgga cgagacgtaa ctacgttatt aaacacggtc agattcggat
    13381 cggttttatg cagcgcatcc agctccggcg tggtttcacg cggataaccg tacagactca
    13441 tgcgtccgcg ctgggtcgac tcgccaatca ccagcactaa agtgcgcggt tcgttacccg
    13501 attcatcttt gaaattagcc agtggcggca aggcattatt ttcattcagt aactttgtta
    13561 gcgagtttag ttgctgacga tactgataat agccggtcag gaattgccac ggtgcggcag
    13621 gctccatacg cgaggccagg ttatccaacg ttttctcaaa cggcttgttt ttgataaacg
    13681 tattcatggc gatcggatgc agaatcaagc cataaagcag ggcaaaagag acaacataac
    13741 gccacggctt tggaatatag accgggcgca ggcgtgtcca cagcagaact gccaccgccg
    13801 tataggccag cgcgataagc acaattttca ggctgaaata ctggcttaaa tactcgctgg
    13861 cttcgttggt gttggtttcg aacatcacaa acagaacgct ctgcgagaac tcctgcccgt
    13921 agatgacgta gtagcatagc gccgccagag aggccgccca tagcaccacg ccgattactg
    13981 cggcaataat tttaatccgc ttcggaaaga ggaataccgg gatcaaccac agcgagctga
    14041 ataacagcga gtcgcgaatg ccgttggtgc cactataacc actgatgtaa ataatggcct
    14101 gtagcagagt agagaaaaac caaaagtaga gcagtgccca acccagggct ttccagctaa
    14161 aaaggggttt agcctggact tctgtggaat gcatagtaag aacctgtctt gaaaaaaata
    14221 tcgccgaatg taacgacaat tccttaagga tatctgaagg tatattcaga atttgaataa
    14281 aatgcagaca gaaatatatt gaaaacgagg gtgttagaac agaagtattt cagaaaaccc
    14341 tcgcgcaaaa gcacgagggt ttgcagaaga ggaagattag ccggtattac gcatacctgc
    14401 cgcaatcccg gcaatagtga ccattaacgc ttgttcgacg cgaggatccg gttcctggcc
    14461 ttctttttct gcctggcggg agcggtgcag caactcggcc tgcaatacgt tcagcgggtc
    14521 ggtgtaaata ttccgtagct gaatagactc tgcaatccac ggcagatcgg ccatcagatg
    14581 ggaatcgttg gcaatcgcca gcaccacttt gatgtcttct tcttgcaggt tgcgtaactc
    14641 tttacctaac ggccacagtg ctttgtctac cagacgttga tcatagtatt ccgccaacca
    14701 caggtctgct ttggcgaaga ccatctccag catgccgaga cgcgtcgaga agaatggcca
    14761 atcgcggcac atggcttcca gctcgctctg tttgccatct tcgaccactt tttgcagcgc
    14821 cgtacctgca cccagccagg cggggagcat caggcggttt tgcgtccagg caaaaatcca
    14881 cggtatggcg cgcagcgact cgacgccgcc ggttgggcga cgtttcgccg gacgtgaacc
    14941 caacggcagt ttgcccaatt cttgttccgg cgtagcggag cggaagtaag gcacaaaatc
    15001 tttgttttca cgtacgtagc cacggtagag atcgcaggag atgactgaca gttcatccat
    15061 aatgcgacgc cagctctctt tcggctcagg tggtggcagc aggttggctt ccagaatcgc
    15121 cccggtgtaa agcgacaggc tgctgacggt gatttctggc agaccatatt taaagcggat
    15181 catctcgccc tgttcagtca cgcgcaggcc acctttcagg cttcctggcg gttgtgacag
    15241 cagcgccgca tgggcaggtg cgccgccgcg accaatggaa ccgccgcgac cgtggaacaa
    15301 cgtcagctca atacccgctt tttcgcaggt tttgattaat gcatcctgtg cctgatattg
    15361 cgcccaggaa gctgccatca cgcccgcatc ttttgctgag tcggaatagc caatcatcac
    15421 catctgtttg ccctgaatca ggccgcgata ccagtcgata ttgagcagct gggtcatgac
    15481 atcgttggcg ttgttcaggt catcgagggt ttcaaacagc ggagcaaccg gcatcgcaaa
    15541 cccgataccc gcttctttca gcagcaggtg gacagccagt acgtcggacg gcgttttcgc
    15601 catcgagatc acgtaggcgg caatggagcc ttgcggcgct tcggcaatca cctggcaggt
    15661 atcgagcact tcgcgcgttt cggcgcttgg ttgccagttg cgcggcagaa gcggacgttt
    15721 ggagttcagt tcgcggatca ggaacgcctg tttgtcggcc tctgaccagc tttcgtagtc
    15781 gccgataccg aggtagcggg tcagctcgcc cagcgcttcg gtatggcggg tgctctcctg
    15841 acggatatca atacggacca gcggtacgcc gaaacatttc acgcggcgca gggtgtcgag
    15901 cagatcgccg ttggcgataa tacccatgcc acacgcctga agtgactggt agcaagcgta
    15961 gagcggttcc cacagttctt cgttttgtgt cagcagccct tctggttttg gcagttcttc
    16021 gcctttcagg cgcgcttcca gccatgcctg tgtcgccatc aggcgagaac gcaggttttt
    16081 catcagatag cgatacggtt ctgcggcacc ttcttcgcca accagcgcca gcagttcagg
    16141 ggtcgcttca accatcgaca gttcagaaac cagaacctga atatctttca ggaacagatc
    16201 ggtggctttc cagcgactga gtagcaggac gtggcgggtg atatcggcag tgacgttcgg
    16261 gttgccgtcg cggtcaccgc ccatccacga agtaaaacgg accggaacaa attcgacggg
    16321 cagtttgtag ccgaggttct cttccagttg ttcgttcagt tcgcgcaagt aattcggtac
    16381 gccttgccac aggctgtttt ctactacggc aaagccccat ttggcttcat ctaccgggct
    16441 tggacgcagc ttacggattt catcagtatg ccatgactgg gcgattaact ggcgcaggcg
    16501 acgcatcagc tggttgtgtt cgtagtcagc gatatctttg ttatcgagct gttttaagca
    16561 ggcgttcact tccaccattt tgtggatcag tgtacgacgg gtaatttcgg ttgggtgagc
    16621 cgtgaggacc agttccagcg acagcgattc cactgctttt ttgatggtgt cttcgctcag
    16681 ttccggctgg tttttcagtt tgcgcagggt gcgggcgatc acttccgggt tgctggcagc
    16741 ttcgcctttc ggcgaaatgc tgtggtattg ctcggcggtg ttggccaggt tcaggaactg
    16801 actaaacgca cgcgcaacgg gcagcagctc gtcgttcgac aaattttgta aggtggtgag
    16861 caactcctgg cggttagcat cattgccagc gcgtgaagat ttcgacaact tacggatagt
    16921 ttctacgcgt tcaagaatgt gttctcccaa cgcatccttg atggtttctc ccagcacttt
    16981 gccgagcata ctgacattac tacgcaatgc ggaatattgt tcgttcatat taccccagac
    17041 accccatctt atcgtttgat agccctgtat ccttcacgtc gcattggcgc gaatatgctc
    17101 gggctttgct tttcgtcgtc ttttataaag ccacgtaaaa gcggtgacgt caaatgctgc
    17161 gaaatcgctt cagcaaacga ataaatagca ggaatttacg ccattaaatt cacgacgctt
    17221 taaataagcg taacttatgg aaatgttaaa aaatcgccat acataacacc aaaggtgtag
    17281 gtcggataag atgcgcaagc atcgcatccg acattattgc ggcaccggag tttggcaacg
    17341 gtgccggatg cggcgcgagc gccttatccg gcctacgttt taatgccagc aaaaatggtg
    17401 aattacctgg ataatcaact cacgggtggg tttaataaac cgcgtctcca gatattcatc
    17461 aggctgatga gcttgattga tggaacccgg accgagcacc agcgtcgggc ataacgtttg
    17521 aataaacggc gcttcggtac agtaattcac cacttcggtt tttgctccga gcaatttctc
    17581 aaccacttca accagttgat gattcggtgg gcattcatag ccagggatcg gcggatgcag
    17641 ctcgtcgacc gtcagacgac ccggccagcg ttcgctcacc ggagccaatg catcgttgag
    17701 caaaccatta agttcattga gtgtcatgct aggcagcgga cgaatatcca tatgcaactc
    17761 acagcaagcg caaatacggt tagaagcgtc gccaccgtga atatgcccga ggttgagcgt
    17821 agggtatggc acggtaaatg cttcgtagtg ataacgttct ttcaggttat cgcgcaattg
    17881 caaaatatgc ccgatggcgt cgtgcattag ttcgatagcg ttaactccgc gtgctggatc
    17941 gctggagtgc cccgactggc cctgaatacg gatggcgtta gagatatgac ctttatgtgc
    18001 gcgtaccggt tgtagtgacg tcggttcgcc aatgatggcg caatccgggc gcagggcggt
    18061 agtttcggca aaataacgcg ctccggccat actggtttct tcatcagcag tcgccagaat
    18121 gtagagcggt tttttcagtt tcgtgacgtc gacatcgcgt agcgtatcaa ggataaacgc
    18181 aaaaaagcct ttcatgtcgg cggtgcctaa gccgtaaagc ttgccgtcat gctccgtcag
    18241 tgtaaacgga tcgcgcgtcc agcgaccgtc atcaaatggc accgtatcgg tatgccccgc
    18301 cagcaacaag ccgccagccc cctgtccgat acttgccagc atattgaatt tgttgcgagt
    18361 tcctggcaca ggctgcactt ccacattgaa gcctaaatct ttaaaccagt ccgccagcag
    18421 agtgattaaa tctgcattgc tttgatcgag tgcctcttcc gtggcgctta ttgaaggtgt
    18481 ggcaatcaga gcacggtaaa tctcgataaa tggcggtaat ttgtttttca ttgttgacac
    18541 acctctggtc atgatagtat caatattcat gcagtattta tgaataaaaa tacactaacg
    18601 ttgagcgtaa taaaacccac cagccgtaag gtgaatgttt tacgtttaac ctggcaacca
    18661 gacataagaa ggtgaatagc cccgatgttg aatacgctga ttgtgggtgc cagcggctac
    18721 gctggcgcag agctagtgac ctatgtaaat cgccatccgc atatgaacat aaccgctttg
    18781 actgtttcag cgcaaagcaa tgatgcggga aagttaatct ccgatttgca tccgcagcta
    18841 aaaggcatcg ttgagctgcc gttgcagccg atgtcggata tcagcgagtt tagcccaggg
    18901 gtggacgtag tgtttctcgc caccgcccat gaagttagcc acgatttagc gccgcagttt
    18961 cttgaagcgg gctgcgtggt gttcgacctt tccggtgcgt ttcgtgttaa cgacgccacc
    19021 ttctatgaaa aatattacgg ctttacccat caatacccgg aactgctgga acaggcagcc
    19081 tacggtctgg cggagtggtg cggtaataaa ttaaaagaag cgaatttgat tgcggtgcca
    19141 ggctgttatc cgacggcggc acagctggcg ctgaaaccgt tgattgatgc cgatcttctt
    19201 gacctcaatc agtggccggt gatcaacgcc accagcggcg tgagcggtgc aggacgtaag
    19261 gcagcgattt caaatagttt ttgtgaagtt agcctgcaac cgtatggcgt ctttactcat
    19321 cgccatcaac cagagatcgc cacacacctc ggtgctgacg ttatcttcac cccacatctg
    19381 ggcaatttcc cgcgcggcat tctcgaaacc attacctgcc gcctgaaatc gggtgtgacc
    19441 caggcgcaag tcgcgcaagt gttacagcag gcgtatgccc ataaaccgct ggtgcggctg
    19501 tatgacaaag gcgttccggc gctgaaaaat gtcgttgggc tgccattttg cgatatcggg
    19561 tttgccgttc agggcgagca tctgattatt gtggcgaccg aagacaactt actgaaaggc
    19621 gcggcggcac aagcggtaca gtgcgccaat attcgtttcg gctatgcgga aacgcagtct
    19681 cttatttaag ggtgcaatga tgaatccatt aattatcaaa ctgggcggcg tactgctgga
    19741 tagtgaagag gcgctggaac gtctgtttag cacactggtg aattatcgtg agtcacatca
    19801 gcgtccgctg gtgattgtgc acggcggcgg ttgcgtggtg gatgagctga tgaaagggct
    19861 gaatctgccg gtgaaaaaga aaaacggcct gcgggtgacg cctgctgatc agatagacat
    19921 tatcaccgga gcactggcgg gaacggcaaa taaaaccctg ttggcatggg cgaagaaaca
    19981 tcagattgcg gccgtaggtt tgtttctcgg tgacggcgac agcgtcaaag tgacccagct
    20041 tgatgaagag ttaggtcatg ttggactggc gcagccaggt tcgcctaagc ttatcaactc
    20101 cttgctggag aacggttatc tgccggtggt cagctccatt ggcgtaacag acgaagggca
    20161 actgatgaac gtcaatgccg accaggcggc aacggcgctg gcggcaacgc tgggcgcgga
    20221 tctgattttg ctctccgacg tcagcggcat tctcgacggc aaagggcaac gcattgccga
    20281 aatgaccgcc gcgaaagcag aacaactgat tgagcagggc attattactg acggcatgat
    20341 agtgaaagtg aacgcggcgc tggatgcggc ccgcacgctg ggccgtccgg tagatatcgc
    20401 ctcctggcgt catgcggagc agcttccggc actgtttaac ggtatgccga tgggtacgcg
    20461 gattttagct taagttttgt tggccggagg cgcagctttc cggcattgaa tttcaaaata
    20521 aggaaacaga gttatggcac tttggggcgg gcgttttacc caggcagcag atcaacggtt
    20581 caaacaattc aacgactcac tgcgctttga ttaccgtctg gcggagcagg atattgttgg
    20641 ctctgtggcc tggtccaaag ccctggtcac ggtaggcgtg ttaaccgcag aagagcaggc
    20701 gcaactggaa gaggcgctga gcgtgctgct ggaagatgtt cgcgccaggc cacaacaaat
    20761 ccttgaaagc gacgccgaag atatccatag ctgggtggaa ggcaaactga tcgacaaagt
    20821 gggccagtta ggcaaaaagc tgcataccgg gcgtagtcgt aacgatcagg tagcgactga
    20881 cctgaaactg tggtgcaaag ataccgttag cgagttactg acggctaacc gacagctgca
    20941 atcggcgctg gtggaaaccg cacaaaacaa tcaggacgcg gtcatgccag gttacactca
    21001 cctgcaacgc gcccagccgg tgacgttcgc gcactggtgc ctggcctatg ttgagatgct
    21061 ggcgcgtgat gaaagccgtt tgcaggatgc gcttaagcgt ctggatgtca gcccattagg
    21121 ctgtggcgcg ctggcgggaa cggcctatga aatcgaccgt gaacagttag caggctggct
    21181 gggctttgct tcggcgaccc gtaacagtct ggacagcgtt tctgaccgtg accatgtgtt
    21241 ggaactgctg tcggctgccg ctatcggcat ggtgcatctg tcgcgttttg ctgaagatct
    21301 gattttcttt aacaccggcg aagcggggtt tgtggagctt tctgaccgcg tgacttccgg
    21361 ttcatcatta atgccgcaga agaaaaaccc ggatgcgctg gagctgattc gcggtaaatg
    21421 cggtcgggtg cagggtgcgt taaccggcat gatgatgacg ctgaaaggtt tgccgctggc
    21481 ttacaacaaa gatatgcagg aagacaaaga aggtctgttc gacgcgctcg atacctggct
    21541 ggactgcctg catatggcgg cgctggtgct ggacggcatt caggtgaaac gtccacgttg
    21601 ccaggaagcg gctcagcagg gttacgccaa cgccaccgaa ctggcggatt acctggtggc
    21661 gaaaggcgta cctttccgcg aggcgcacca tatcgtgggt gaagcggtgg tggaagccat
    21721 tcgtcagggc aaaccgctgg aagatctgcc gctcgacgag ttgcagaaat tcagcccggt
    21781 gattggcgaa gatgtctatc cgattttgtc gctgcaatcg tgcctcgaca agcgtgcggc
    21841 aaaaggcggc gtctcaccgc agcaggtggc ccaagcgatt gcttttgcgc aggcgcggtt
    21901 ggggtaagaa catttatatg tacaaatttg agcctggctt atcgccgggc ttttttatgg
    21961 caaaaaaaag cggatcctgg agatccgcaa aagttcacgt tggctttagt tattcgagtt
    22021 gagaaactct cgaaacgggc agtgacttca agggttaaaa gaggtgccgc tccgtttctg
    22081 tgagcaatta tcagtcagaa tgcttgatag ggataatcgt tcattgctat tctacctatc
    22141 gccatgaact atcgtggcga tggaggatgg ataatgaata ttcgtgatct tgagtacctg
    22201 gtggcattgg ctgaacaccg ccattttcgg cgtgcggcag attcctgcca cgttagccag
    22261 ccgacgctta gcgggcaaat tcgtaagctg gaagatgagc tgggcgtgat gttgctggag
    22321 cggaccagcc gtaaagtgtt gttcacccag gcgggaatgc tgctggtgga tcaggcgcgt
    22381 accgtgctgc gtgaggtgaa agtccttaaa gagatggcaa gtcagcaggg cgagacgatg
    22441 tccggaccgc tgcatattgg tttgattccc acggttggac cgtacctgct accgcatatt
    22501 atcccgatgc tgcaccagac ctttccaaag ctggaaatgt atctgcatga agcacagacc
    22561 caccagttac tggcgcaact ggacagtggc aaactcgatt gcgtgatcct cgcgctggtg
    22621 aaagagagcg aagcattcat tgaagtgccg ttgtttgatg agccaatgtt gctggctatc
    22681 tatgaagatc acccgtgggc gaaccgcgaa tgcgtaccga tagccgatct ggcaggggaa
    22741 aaactgctga tgctggaaga tggtcactgt ttgcgcgatc aggcaatggg tttctgtttt
    22801 gaagccgggg cggatgaaga tacacacttc cgcgcgacca gcctggagac gctgcgcaac
    22861 atggtggcgg caggtagcgg gatcacttta ctgccagcgc tggctgtgcc gccggagcgc
    22921 aaacgcgatg gggttgttta tctgccgtgc attaagccgg aaccacgccg cactattggc
    22981 ctggtttatc gtcctggctc accgctgcgc agccgctatg agcagctggc agaggccatc
    23041 cgagcaagaa tggatggcta tttcgataaa gtgttaaaac aggcggttta aaccgtttaa
    23101 cgcagctacc cgataggctt ccgccatggt cgggtagtta aaggtggtgt tgacgaagta
    23161 ctcaatagtg ttgccgccac ctttctgttc cataatcgcc tgaccgatat gaataatttc
    23221 ggcagcgcgc tcgccaaagc agtgaatacc cagaatctct tttgtttccc gatggaacaa
    23281 aattttcagc gtgcccacgt tcatgccgac gatttgtgcg cgtgccagat gtttaaactg
    23341 ggcgcggccc acttcatatg gcactttcat cgcggtcagc tgctgttcgg ttttgcccac
    23401 agagctgatt tccgggatgg tgtaaatgcc ggtagggata tcttcaatca gatgcgcggt
    23461 ggcttcgcct tttaccagcg cctgcgcggc aatgcgcccc tggtcatagg ccgctgatgc
    23521 caggctcgga taaccaatca cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata
    23581 catgctgttg accttcagct gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc
    23641 cagcgaatcg gtattaccgg tacgaccgtt ggcatagagc aggcagtcag ctttcagttt
    23701 tttacccgac ttcagatgca tgatcacacc gtcgtcacag ccttcgatct tctcgtactc
    23761 ttcgttgtga cgaatcacta cgccactgtt ccagaagtga taggagagag aatctgacat
    23821 ctcttgatcg agaaatgcca gcaggcgatc gcgggtgttg atcagatcca cttttacatc
    23881 cataccgcgg aagatcgacg catattcaca gccgatcact ccagcaccat agataagtac
    23941 atggcgcggt tcgtggtgca tgctaagaat tgagtcgctg tcgtaaatgc gtggatgggt
    24001 gaaatcaaca tctgttggat gatatggacg agagccgcag gcaataacaa atttttcagc
    24061 ggttagtgtt tcgacgctgc cgtccgggca atccagcgcc aacgtatgct cgtcaacaaa
    24121 gcgagcgttc ccctgcaata tttcacagtg attacgttcg taaaatccct gacgcatgcg
    24181 cgtttgttga ttaatcacgt tatcggcatg gttaaggata tcggcaaaag aagagcggag
    24241 cagtcgcgaa tggtcgctgt atagtgggtt ttgattgaat tctataatgc ggctgacggc
    24301 gtgacggaga gctttcgacg ggatggtgcc ccagtgggtg caaccgccgc caacattttg
    24361 ataacgctcg ataactgcga cgcgcacacc ttgcttaacc aggcccattg cagcgccttc
    24421 gccgccgggg ccggaaccta ttactatggc atcgtaatcg taggaatgtg gcatggtagg
    24481 gcttacctgt tcttatacat aaaagcaaca gaatggtaac attctatcgc gggtaagcca
    24541 attgatcccc gtcatttatc tggctatatc ctgagcggcc tttgctttgt ctgtttctta
    24601 cttttgccct gacgttttat tggattttta tcgacgatac tctccgttta agcggcaggt
    24661 ttccgctgta cgtaaaagaa ccggccagta aatatgtttt attgcgttac cgttcattca
    24721 caatactgga gcaatccagt atgttcattc tctggtatag tgccagcagt acttttggca
    24781 aggattcaga catcgtgatg ggcataagag cgcaacaaaa agaaaaaacc cgccgttcgc
    24841 tggtggaagc cgcatttagc caattaagtg ctgaacgcag cttcgccagc ctgagtttgc
    24901 gtgaagtggc gcgtgaagcg ggcattgctc ccacctcttt ttatcggcat ttccgcgacg
    24961 tagacgaact gggtctgacc atggttgatg agagcggttt aatgctacgc caactcatgc
    25021 gccaggcgcg tcagcgtatc gccaaaggcg ggagtgtgat ccgcacctcg gtctccacat
    25081 ttatggagtt catcggtaat aatcctaacg ccttccggtt attattgcgg gaacgctccg
    25141 gcacctccgc tgcgtttcgt gccgccgttg cgcgtgaaat tcagcacttc attgcggaac
    25201 ttgcggacta tctggaactc gaaaaccata tgccgcgtgc gtttactgaa gcgcaagccg
    25261 aagcaatggt gacaattgtc ttcagtgcgg gtgccgaggc gttggacgtc ggcgtcgaac
    25321 aacgtcggca attagaagag cgactggtac tgcaactgcg aatgatttcg aaaggggctt
    25381 attactggta tcgccgtgaa caagagaaaa ccgcaattat tccgggaaat gtgaaggacg
    25441 agtaatgaaa caagcaaatc aagatagagg tacgctgctg ctggcgttag ttgctggctt
    25501 atcgattaat ggtactttcg cagcgctgtt tagctccatt gtgccatttt ctgtattccc
    25561 gattatttcc ctggtgctga cggtttactg cctgcatcaa cgttatctta atcgcactat
    25621 gccggtaggc ttgccgggtc tggcagctgc ctgttttatt ctcggcgtac tgctgtacag
    25681 cacggtagtt cgtgcggaat atccggatat cggctctaac ttcttcccgg cagtactctc
    25741 cgtcattatg gtgttctgga ttggcgcgaa gatgcgtaac cgtaagcagg aagttgctga
    25801 gtaatcggta ttatgccgga tgctggcgca tccggcatgg gttttacttc gcggtcagta
    25861 atacgccgca ctccatatgg tgcgtgtagg ggaactgatc aaacagagcc agacgttcga
    25921 ccttgtgcgt ctggcttaat gtttccagat tcttgcataa cgtttccggg ttacaggaga
    25981 tgtacaaaat acacggatac gcctgcacca ttttctcggt ttcactgtcc agaccgctgc
    26041 gcggtgggtc gacaaaaatg gtttcgcact gataactctt taagtcgatc ccttgcaggc
    26101 ggttaaactc gcgcacacca ttcatcgcct gagtaaattc ttctgccgcc atacgaataa
    26161 tttgtacgtt atcaatatgg ttagctgcga tgttgtattg cgcagcagca accgacggct
    26221 tagcgatttc ggtggctaat acccgatcaa aattgcgcgc cagcgctaat gaaaagttac
    26281 cgttgccgca gtacagctcc agtaaatcgc cttttgagcc tttggttacg tccagcgccc
    26341 attccagcat ctgaatattc atcgccgcgt tcggctgggt aaagctgttt tctacctgac
    26401 ggtagatcat ctctttccct gcgaccggca gacgctcgtc gatgtaatct tgatccagct
    26461 cgattttggt tttcgttgcc cgaccaatca gatgcacatt cagattctgc gcgcgcagtg
    26521 catcgcgcag ggcctccgct tcctgacgcc actcatcatc cagcttctta tggtatagca
    26581 gggaaaccac cgcctgatta ctcagcgtgg tgaggtaatc aatctggaac aacttgtggc
    26641 gcagaacggg attattacgc acacccgcaa tcatcgccgt catcaactgg ttgataagtt
    26701 cactggcggc ggggaagcta tccacgcgga tacggctttt ggtttgttga tcgaaaatga
    26761 tgtgatacag gtcatcgcca tcgtgccaga tgcggaactc cgcacgcatc cggtaatgac
    26821 tgaccggcga gcgaaacact tccggaacca ggtcagaaaa cggtgccatc atactttgca
    26881 aacgtaccac tttttcggct aactgcgctt catactgttc tgttggaagg tgttcggggg
    26941 tcatgatgta tcctgaaaaa ttaaagtacg cggcgattgt agggattgct catcagatgt
    27001 ccagatcttg atgaattcct atttgtgagc tacgtctgga cagtaacttg ttacaacctg
    27061 tagcatccac ttgtcggtcc tgtgagttaa tagggaatcc agtgcgaatc tggagctgac
    27121 gcgcagcggt aaggaaaggt gcgatgattg cgttatgcgg acactgccat ccggtgggaa
    27181 gtcatcatct cttagtatct tagatacccc tccaagcccg aagacctgcc ggccaacgtc
    27241 gcatctggtt ctcatcatcg cgtaatattg atgaaacctg cggcatcctt cttctattgt
    27301 ggatgcttta caatgattaa aaaagcttcg ctgctgacgg cgtgttctgt cacagccttt
    27361 tccgcttggg cacaggatac cagcccggat actctcgtcg ttactgctaa ccgttttgaa
    27421 cagccgcgca gcactgtgct tgcaccaacc accgttgtga cccgtcagga tatcgaccgc
    27481 tggcagtcga cctcggtcaa tgatgtgctg cgccgtcttc cgggcgtcga tatcacccaa
    27541 aacggcggtt caggtcagct ctcatctatt tttattcgcg gtacaaatgc cagtcatgtg
    27601 ttggtgttaa ttgatggcgt acgcctgaat ctggcggggg tgagtggttc tgccgacctt
    27661 agccagttcc ctattgcgct tgtccagcgt gttgaatata tccgtgggcc gcgctccgct
    27721 gtttatggtt ccgatgcaat aggcggggtg gtgaatatca tcacgacgcg cgatgaaccc
    27781 ggaacggaaa tttcagcagg gtggggaagc aatagttatc agaactatga tgtctctacg
    27841 cagcaacaac tgggggataa gacacgggga acgctgttgg gcgattatgc ccatactcat
    27901 ggttatgatg ttgttgccta tggtaataca ggaacgcaag cgcagacaga taacgatggt
    27961 tttttaagta aaacgcttta tggcgcgctg gagcataact ttactgatgc ctggagcggc
    28021 tttgtgcgcg gctatggcta tgataaccgt accaattatg acgcgtatta ttctcccggt
    28081 tcaccgttgc tcgatacccg taaactctat agccaaagtt gggacgccgg gctgcgctat
    28141 aacggcgaac tgattaaatc acaactcatt accagctata gccatagcaa agattacaac
    28201 tacgatcccc attatggtcg ttatgattcg tcggcgacgc tcgatgagat gaagcaatac
    28261 accgtccagt gggcaaacaa tgtcatcgtt ggtcacggta gtattggtgc gggtgtcgac
    28321 tggcagaaac agactacgac gccgggtaca ggttatgttg aggatggata tgatcaacgt
    28381 aataccggca tctatctgac cgggctgcaa caagtcggcg attttacctt tgaaggcgca
    28441 gcacgcagtg acgataactc acagtttggt cgtcatggaa cctggcaaac cagcgccggt
    28501 tgggaattca tcgaaggtta tcgcttcatt gcttcctacg ggacatctta taaggcacca
    28561 aatctggggc aactgtatgg cttctacgga aatccgaatc tggacccgga gaaaagcaaa
    28621 cagtgggaag gcgcgtttga aggcttaacc gctggggtga actggcgtat ttccggatat
    28681 cgtaacgatg tcagtgactt gatcgattat gatgatcaca ccctgaaata ttacaacgaa
    28741 gggaaagcgc ggattaaggg cgtcgaggcg accgccaatt ttgataccgg accactgacg
    28801 catactgtga gttatgatta tgtcgatgcg cgcaatgcga ttaccgacac gccgttgtta
    28861 cgccgtgcta aacagcaggt gaaataccag ctcgactggc agttgtatga cttcgactgg
    28921 ggtattactt atcagtattt aggcactcgc tatgataagg attactcatc ttatccttat
    28981 caaaccgtta aaatgggcgg tgtgagcttg tgggatcttg cggttgcgta tccggtcacc
    29041 tctcacctga cagttcgtgg taaaatagcc aacctgttcg acaaagatta tgagacagtc
    29101 tatggctacc aaactgcagg acgggaatac accttgtctg gcagctacac cttctgaacc
    29161 acgtcccacc gtgctggtgt ttgactccgg cgtcggtggg ttgtcggtct atgacgagat
    29221 ccggcatctc ttaccggatc tccattacat ttatgctttc gataacgtcg ctttcccgta
    29281 tggcgaaaaa agcgaagcgt ttattgttga gcgagtggtg gcaattgtca ccgcggtgca
    29341 agaacgttat ccccttgcgc tggctgtggt cgcttgcaac actgccagta ccgtttcact
    29401 tcctgcatta cgcgaaaagt tcgacttccc ggttgttggt gtcgtgccgg cgattaaacc
    29461 tgctgcacgt ctgacggcaa atggcattgt cggattactg gcaacccgcg gaacagttaa
    29521 acgttcttat actcatgagc tgatcgcgcg tttcgctaat gaatgccaga tagaaatgct
    29581 gggctcggca gagatggttg agttggctga agcgaagcta catggcgaag atgtttctct
    29641 ggatgcacta aaacgtatcc tacgcccgtg gttaagaatg aaagagccgc cagataccgt
    29701 tgtattgggt tgcacccatt tccctctact acaagaagaa ctgttacaag tgctgccaga
    29761 gggaacccgg ctggtggatt ctggcgcagc gattgctcgc cgaacggcct ggttgttaga
    29821 acatgaagcc ccggatgcaa aatctgccga tgcgaatatt gccttttgta tggcaatgac
    29881 gccagaagct gaacaattat tgcccgtttt acagcgttac ggcttcgaaa cgctcgaaaa
    29941 actggcagtt ttaggctgat ttggttgaat gttgcgcggt cagaaaatta ttttaaattt
    30001 cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg aacaacggca
    30061 aacacgccgc cgggtcagcg gggttctcct gagaatctca acaga
//
LOCUS       DAZOYL010000015        29548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZOYL010000015 DAZOYL010000000
VERSION     DAZOYL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.015"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHUOY_000497"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHUOY_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR0681382.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHUOY_000498"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHUOY_000498"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR0681383.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUOY_000499"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUOY_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR0681384.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHUOY_000500"
     CDS             complement(3644..4030)
                     /locus_tag="ACHUOY_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR0681385.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUOY_000501"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUOY_000501"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR0681386.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUOY_000502"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUOY_000502"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR0681387.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUOY_000503"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUOY_000503"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR0681388.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUOY_000504"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUOY_000504"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR0681389.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUOY_000505"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUOY_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR0681390.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUOY_000506"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUOY_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR0681391.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUOY_000507"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUOY_000507"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR0681392.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHUOY_000508"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHUOY_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR0681393.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUOY_000509"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUOY_000509"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR0681394.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUOY_000510"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUOY_000510"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR0681395.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUOY_000511"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUOY_000511"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR0681396.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUOY_000512"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUOY_000512"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR0681397.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUOY_000513"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUOY_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR0681398.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHUOY_000514"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHUOY_000514"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR0681399.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUOY_000515"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUOY_000515"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR0681400.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUOY_000516"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUOY_000516"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR0681401.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUOY_000517"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUOY_000517"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR0681402.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUOY_000518"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUOY_000518"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR0681403.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUOY_000519"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUOY_000519"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR0681404.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUOY_000520"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUOY_000520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0681405.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHUOY_000521"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHUOY_000521"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR0681406.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7120 c         8160 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZOYL010000016        28517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZOYL010000016 DAZOYL010000000
VERSION     DAZOYL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.016"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(285..512)
                     /locus_tag="ACHUOY_000522"
     CDS             complement(285..512)
                     /locus_tag="ACHUOY_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR0681407.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            704..1909
                     /gene="csdA"
                     /locus_tag="ACHUOY_000523"
     CDS             704..1909
                     /gene="csdA"
                     /locus_tag="ACHUOY_000523"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR0681408.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            1909..2352
                     /gene="csdE"
                     /locus_tag="ACHUOY_000524"
     CDS             1909..2352
                     /gene="csdE"
                     /locus_tag="ACHUOY_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR0681409.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2403..3209)
                     /gene="tcdA"
                     /locus_tag="ACHUOY_000525"
     CDS             complement(2403..3209)
                     /gene="tcdA"
                     /locus_tag="ACHUOY_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR0681410.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3286..4383)
                     /gene="mltA"
                     /locus_tag="ACHUOY_000526"
     CDS             complement(3286..4383)
                     /gene="mltA"
                     /locus_tag="ACHUOY_000526"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR0681411.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            4592..4668
                     /locus_tag="ACHUOY_000527"
     tRNA            4592..4668
                     /locus_tag="ACHUOY_000527"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4626..4628,aa:Met,seq:cat)
     gene            complement(4743..5996)
                     /gene="amiC"
                     /locus_tag="ACHUOY_000528"
     CDS             complement(4743..5996)
                     /gene="amiC"
                     /locus_tag="ACHUOY_000528"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR0681412.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            6228..7559
                     /gene="argA"
                     /locus_tag="ACHUOY_000529"
     CDS             6228..7559
                     /gene="argA"
                     /locus_tag="ACHUOY_000529"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR0681413.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7621..9447)
                     /gene="recD"
                     /locus_tag="ACHUOY_000530"
     CDS             complement(7621..9447)
                     /gene="recD"
                     /locus_tag="ACHUOY_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR0681414.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKRRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(9447..12989)
                     /gene="recB"
                     /locus_tag="ACHUOY_000531"
     CDS             complement(9447..12989)
                     /gene="recB"
                     /locus_tag="ACHUOY_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR0681415.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12982..15870)
                     /gene="ptrA"
                     /locus_tag="ACHUOY_000532"
     CDS             complement(12982..15870)
                     /gene="ptrA"
                     /locus_tag="ACHUOY_000532"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR0681416.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(16046..19414)
                     /gene="recC"
                     /locus_tag="ACHUOY_000533"
     CDS             complement(16046..19414)
                     /gene="recC"
                     /locus_tag="ACHUOY_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR0681417.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(19427..19750)
                     /locus_tag="ACHUOY_000534"
     CDS             complement(19427..19750)
                     /locus_tag="ACHUOY_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR0681418.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19735..20142)
                     /locus_tag="ACHUOY_000535"
     CDS             complement(19735..20142)
                     /locus_tag="ACHUOY_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR0681419.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(20139..20702)
                     /locus_tag="ACHUOY_000536"
     CDS             complement(20139..20702)
                     /locus_tag="ACHUOY_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0681420.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(20693..21163)
                     /locus_tag="ACHUOY_000537"
     CDS             complement(20693..21163)
                     /locus_tag="ACHUOY_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0681421.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(21348..22142)
                     /gene="thyA"
                     /locus_tag="ACHUOY_000538"
     CDS             complement(21348..22142)
                     /gene="thyA"
                     /locus_tag="ACHUOY_000538"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0681422.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(22149..23024)
                     /gene="lgt"
                     /locus_tag="ACHUOY_000539"
     CDS             complement(22149..23024)
                     /gene="lgt"
                     /locus_tag="ACHUOY_000539"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR0681423.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(23175..25421)
                     /gene="ptsP"
                     /locus_tag="ACHUOY_000540"
     CDS             complement(23175..25421)
                     /gene="ptsP"
                     /locus_tag="ACHUOY_000540"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0681424.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(25434..25964)
                     /gene="rppH"
                     /locus_tag="ACHUOY_000541"
     CDS             complement(25434..25964)
                     /gene="rppH"
                     /locus_tag="ACHUOY_000541"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR0681425.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(26317..26463)
                     /gene="ygdT"
                     /locus_tag="ACHUOY_000542"
     CDS             complement(26317..26463)
                     /gene="ygdT"
                     /locus_tag="ACHUOY_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR0681426.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            26649..27338
                     /gene="mutH"
                     /locus_tag="ACHUOY_000543"
     CDS             26649..27338
                     /gene="mutH"
                     /locus_tag="ACHUOY_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR0681427.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            27407..28120
                     /locus_tag="ACHUOY_000544"
     CDS             27407..28120
                     /locus_tag="ACHUOY_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0681428.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            28258..28476
                     /gene="ygdR"
                     /locus_tag="ACHUOY_000545"
     CDS             28258..28476
                     /gene="ygdR"
                     /locus_tag="ACHUOY_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR0681429.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         6811 a         7789 c         7143 g         6774 t
ORIGIN      
        1 ttaatctgag ccattataaa ttgtccgttg agcttctacc agcaaatacc tatagtggcg
       61 gcacttcctg agccggaacg aaaagtttta tcggaatgcg tgttctggtg aacttttggc
      121 ttacggttgt gatgttgtgt tgttgtgttt gcaattggtc tgcgattcag accatggtag
      181 caaagctacc ttttttcact tcctgtacat ttaccctgtc tgtccatagt gattaatgta
      241 gcaccgccta attgcggtgc ttttttttac cttgcgatcg cgaattactg atccagttcg
      301 accatctctt tcacgtcagt acggttgatc tgctgtttgt tgccattagc gtctttatac
      361 gaaatcatac cggtatcgtt atcagtctgt ggtttgccgt cagagacgat ggtacgtccg
      421 tcattggtgt gcatcacata gttcgaaccg gaacaggcgc tcagggcaaa agtcagcata
      481 caggcagaaa taattgcggc agtctttttc atcatcttct ccttaaagcg attaataaat
      541 cttaatagta ttgattaaca ggctaaaatt aacgcctaac actattcagc atatgttact
      601 tacgcggctt cgccaggata tccagataat tctgatggtt agcactctcc ttgtatcaaa
      661 gtgaattttg cgtcacgatc ggtgcatcaa gccgaggagt accatgaacg tttttaatcc
      721 cgcgcagttt cgcgcccagt ttcccgcact acaggatgcg ggcgtctatc tcgatagcgc
      781 cgcgaccgcg cttaaacctg aagccgtggt tgaagccact cgacagtttt acagcctgag
      841 cgccggaaac gtccatcgca gccagtttgc cgaagcccaa cgcctgaccg cgcgttatga
      901 agctgcacga gagaaagtgg cgcaattact gaatgcaccg gatgataaaa ctatcgtctg
      961 gacgcgcggc accactgaat ccatcaacat ggtggcacaa tgctatgcgc gtccgcgtct
     1021 gcaaccgggc gatgagatta ttgtcagcgt ggcagaacac cacgctaacc tcgtcccctg
     1081 gctgatggtc gcccaacaaa ctggagccaa agtggtgaaa ttgccgctta atgcgcagcg
     1141 actgccggat gtcgatttgt tgccagaact gattactccc cgtagtcgga ttctggcgtt
     1201 gggtcagatg tcgaacgtta ctggcggttg cccggatctg gcgcgagcga ttacctttgc
     1261 tcattcagcc gggatggtgg tgatggttga tggtgctcag ggggcggtgc atttccccgc
     1321 agatgttcag caactagata tcgatttcta tgctttctct ggtcacaaac tgtatggccc
     1381 gacgggtatc ggcgtgctgt atggcaaatc agaactgctg gaagcgatgt cgccctggct
     1441 gggtggcggc aaaatgattc acgaagtgag ttttgacggc ttcacgactc aatctgcgcc
     1501 gtggaaactg gaagcaggaa cgccaaatgt ggctggtgtc ataggattaa gcgcggcgct
     1561 ggaatggctg gcagattacg atatcaacca ggcagaaagt tggagtcgca gcctggcgac
     1621 gctggcggaa gatgcgctgg caaaacgtcc tggcttccgc tcattccgct gccaggattc
     1681 cagcctgctg gcctttgatt ttgctggcgt tcatcacagc gatatggtga cgctgctggc
     1741 ggagtacggt attgccctgc gggccgggca acattgcgct cagccgctac tggcagaatt
     1801 aggcgtaacc ggcacactgc gcgcctcttt tgcgccatat aatacaaaga gtgatgtgga
     1861 tgcgctggtg aatgccgttg accgcgcgct ggaattattg gtggattaat gacgaacccg
     1921 caattcgccg gacatccgtt cggcacaacc gtaaccgcag aaacgttacg caataccttc
     1981 gcaccgttgt cgcaatggga agataaatat cgccagttga tcatgctggg gaaacagctt
     2041 ccggctttgc cagacgagtt aaaagcgcag gctaaagaga ttgccggatg cgaaaaccgc
     2101 gtctggctgg gatataccgt gactgaaaac ggtaaaatgc acttctttgg cgatagcgaa
     2161 gggcgcattg tgcgcggcct gctggcggtg ctgttgactg ccgttgaggg gaaaaccgcc
     2221 gccgaattgc aggcacagtc gccgctggca ttgtttgatg agctgggatt gcgtgcgcaa
     2281 cttagcgcct cacgcagcca ggggttaaat gcgttaagcg aggcgattat cgctgcgacg
     2341 aagcaggttt aagcatagaa ccgggtcgga taagacgttc gcgtctcatc cgacctgatt
     2401 gtttaaccct gacgcgccgc tttcgccatc atcttcttca gcgcatgaga aaccgcaaca
     2461 aacccaaagg tggcggtcac catcgttgcc gcgccaaatc cagatgcaca atccatccgc
     2521 ttcggccctt ctgccgtggc tttcatcgca cacacagtac cgtctgactg cgggtacacc
     2581 agcgcttcag tagaaaacac gcaatcgacg ccgagcttac ctttactgtt tttcactacg
     2641 ccaaaatcgc ttttcaggcg ctcgcgcaac ttcgccgcca gcggatcctg aatcgttttc
     2701 gccagatcgg taacctgaat ctgcgtcggg tcaatctgcc cacccgcgcc accggtcgtg
     2761 accagcggga ttttattgcg ccgacaataa gcaatcagcg ccgctttggg ccgtacacta
     2821 tcaatggcat caatcacgta cgaataaccc acgctcatat actgcgctac gttatccggc
     2881 gtcacgaaat catccaccac cgttacacgg cactccgggt taatctggcg aatacgctcc
     2941 gccatgactt ccgcttttgc cagcccgacg ttatcgcgca gagcgtgaat ttgccgattg
     3001 gtattggtga cgcacacatc atccatatcg ataagcgtga ttgcgccaat ccccgtgcgc
     3061 gccagcgctt ccgccgccca ggaaccgaca ccaccgatac caaccacaca aatatgcgcg
     3121 tcagcaaaca gttgcaacgc tttttcacca tacagacgcg ctgtgccacc aaaacgctga
     3181 cgccatgcat cactaattac cacagacata gcacctcaga attaaaaagg gtgaggttat
     3241 ccctcaccct gtcgtatccg taaaaacggt atacagaata acacatcagc cgctaaagac
     3301 gttacctgcg cccggggcgg ttttcagcac ccagacacgt ccatagtggt tgtaccaacc
     3361 tgcgcggtgt ccggcttccg gcccgatccc ttgatagata tcgaagtgtt ggcctttgat
     3421 tgcaccaccg acatccagcg ccaccatcag acgcagttcg tactgaccat taaatttgcc
     3481 gttattatcc agcaacggca cttctgccag caaggtagta cctggcggaa taatggaacg
     3541 atcagaggca actgacgcgc gaccaaccag cggcaccgca cttgcccctt tcaccggtgc
     3601 aaaagattgc ggtttaaaga agacgaaaga cgggttctgt tccagcagct cgcgaacctc
     3661 ggcttcactg tgtgtttcgc cccagtgacg aatcgcctgc atcgacatat cttctttttt
     3721 cacttcgcca cggtcgatca gcaccttacc aatgctgcga taggcatgac cgtttttccc
     3781 tgcatagctg aaaaagttaa gcggactgcc atcaccaaag tcgatatacc cactaccctg
     3841 cacatccata atgaagttat ccatcaggga gttactgtaa gcgagaatat atttatcact
     3901 caatgccccc gcgtagatct ccgcacgaga cggcagacga ccacgttttg gcggcatacg
     3961 gtaaatagga tactggaact cgccctggcg ggtatggcgc gcctgaatta ccggcgtgta
     4021 atagcctgta aactgcacgt taccatagtt gtcggcacct tccatctgcc aggcatcaat
     4081 gccgaactgg cgcatattgc gggtatcacc gcctgcgcgc agccactctt gcaccgcgtt
     4141 ataaacatta ctctggttgc catacagacg cggtgacgaa ttacggatat ggttaatttg
     4201 ctcggcaaaa tcaccggcgt taatcggcgc gccaacggca tctggctggt tcaccagaga
     4261 gaaaggctgg gtaaatttcc cgtctttata ttgctgtccg cgatcggttg gtttggaaga
     4321 gcaggcggca agcattgcca caaccgtgcc cataagaagg tactttaccc aacgtccttt
     4381 cattgttctc ttcttcaggt taaaaaataa ggcgcaacga agataacaaa ccgccgcgtt
     4441 taatgaaatg agcagcctct ccctgacgca aattttgcac aaaaaatagg ctttagtgat
     4501 ttgtttttgt tcaaaatcat gccagatccg tgatcggggt aaaaaaaagg ttgcatgaaa
     4561 acgcgagcgg agtatagtgc gcatccacgg aggcggggtg gagcagcctg gtagctcgtc
     4621 gggctcataa cccgaaggtc gtcggttcaa atccggcccc cgcaaccaat tattgaacac
     4681 cctaacgggt gtttttttgt ttctactctc ccataaaaaa agcgccattc agcgcctttt
     4741 tatcatcccc ttctcgccag cgttgcccca tcggcaaaat acgctttaat ccccgcaaga
     4801 atagactccg caacttcctg ctggaaagtc gccgttttca gtttacgctc ttcctcaacg
     4861 ttactgataa acgccgtttc gaccagaatg gagggaatat ctggtgcctt tagtacggca
     4921 aacccggcct gttcaacttg atttttatgc agcttgttga ttttaccgag cttattcagc
     4981 actgctttac caaacttaag gctgtcagca atggtcagcg actgcaccat atcgaacatg
     5041 gtgtggtcga catagcggtc accgcttttg ctcacgccac caatcaagtc cgaggcgttc
     5101 tgggtttgtg ccagatattt tgccgcagta ctggttgcac ctttggttga gagcgcaaac
     5161 accgaggaac cgctcggctg gcgactggta aaggcgtcgg catggataga gacaaacagg
     5221 tcagcacgct gtttctgggc ttttgctacg cgcacttgca acggaatgaa gatgtcttca
     5281 ttgcgcgtca tgtacacctt catattgccc tctttctcga tcagagagcg caaacggcga
     5341 gctatttgca ataccacatc tttttcgcgc gttttgtatt tccccaccgc accggagtct
     5401 tcgccaccgt ggccagggtc aagcataatg acaatcggac gatcgcgccc tgctttacct
     5461 ggttgtggac cactttgcgc tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc
     5521 tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat tggccggata gaggtccatc
     5581 accagacgct ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt
     5641 tgctttaatt caaaaaccat ccgtacggtt tgcgggtcaa attgcccgac gcgcgccgac
     5701 ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac cgagttcagg
     5761 tttacatctt cgatatccac caccacgcgt tcagggttac tcaacgcgaa ctgcttatat
     5821 ttcagctgac gatttgattc taccgtcacg cgggtgtagc tggacgcagg ccagacgcgc
     5881 accgccacga cctggctaac cgcagccagg ctgacctgac ttacgctcaa tagccacata
     5941 gcgcccgcgc cttgcagtaa acgacgacgg ctgattgcag tgttggatcc tgacatgcct
     6001 ctcccgagca aaagaaatct aagctgtgta acaagtaaac gactaatttg accggaaact
     6061 ttagcgaaag acgcataatc tgtcatctaa taaacggtaa acattctttt tatattcacg
     6121 gcattactga taaaaaagtc gctctcgcat aaaatttaca cttgcaccct gcgaaaaaac
     6181 agaataaaaa tacaataatt tcgaataatc atgcaaaggg gtgtgccgtg gtaaaggaac
     6241 gtaaaaccga gctggtcgag ggattccgcc attcggttcc ctatatcaat acccaccggg
     6301 gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga gcatgagaat ttctccagta
     6361 tcgttaatga tatcgggttg ttgcacagcc tcggcatccg tctggtggtg gtctatggcg
     6421 cacgtccgca gatcgacgca aatctggctg cgcatcacca cgaaccgctg tatcacaaga
     6481 atatacgtgt gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg ggaacattgc
     6541 aactggatat tactgctcgc ctgtcgatga gtctcaataa cacgccgctg cagggcgcgc
     6601 atatcaacgt cgtcagtggc aattttatta ttgcccagcc gctgggcgtc gatgacggcg
     6661 tggattactg ccatagcggg cgtatccggc ggattgatga agacgcgatc cgtcgtcaac
     6721 tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact ggcgagagct
     6781 ttaatctgac ctcggaagag attgccactc aactggccat caaactgaaa gctgagaaga
     6841 tgattggttt ttgctcttca cagggcgtca ctaatgacga cggtgatatt gtctcagaac
     6901 ttttccctaa cgaagcgcaa gcgcgggtag aagcccagga agagaaaggc gattacaact
     6961 ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc gtgcgtcgct
     7021 gtcatttaat cagttatcag gaagatggcg cgctgttgca agagttgttc tcacgcgacg
     7081 gtatcggtac gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca acaatcaacg
     7141 atattggcgg tattctggag ttgattcgcc cgctggagca gcaaggtatt ctggtgcgcc
     7201 gttctcgcga gcagctggag atggaaatcg acaaattcac cattattcag ctcgataaca
     7261 cgactattgc ctgcgccgcg ctctatccgt tcccggaaga gaagattggg gaaatggcct
     7321 gtgtggcagt tcacccggat taccgcagtt catcacgggg cgaggttctg ctggaacgca
     7381 ttgccgctca ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc acgcgcagta
     7441 ttcactggtt ccaggaacgt ggatttaccc cggtggatat tgatttactg cccgagagca
     7501 aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt gatggcggat ttagggtaag
     7561 cgatgaaaat cgccggatgc ggcaggcgta acactcgtgc gtcgcatccg gcaattacgt
     7621 ttatccccgc gaactaaaca acgccgccag accactgcgc cgctcagtac gagtggcgat
     7681 tgccgcactt aatatacgct catcggcata cagcgacaga cggcgacgcg cgcgggtcac
     7741 cgcggtgtat accagctctc gcgttactat cggcgtgcgt tggctcggta aaatcaacgc
     7801 cgcatggtcg aactccgatc cctgcgattt atgtaccgtc atcgcccacg tagtttcgtg
     7861 ctctggcaag cgactcggtt gcacagactt aatattgccg tccggcatcg caaaccagac
     7921 gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg ccattaaaca acccaagcgc
     7981 gctgtcatta cgggcaatca tcaccggtcg accttcgtac caacgagagt gcgaatgacg
     8041 atgaattttg cgcttctgtt gcataaactg ctcaattcgc tcattcagtc cagccacgcc
     8101 aaatggccct tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct gaatgattaa
     8161 atccggctcg gcacgcgctt gcagcagatc cagataacgt ccataacccg caagagcttc
     8221 ctcaagcatc gcaatataat cttcgccgct ctgtaaaagc cgtttttcaa tatcagtaaa
     8281 atcctgctga aaaacggttt tcactgccgt tttatcacca cggttgatcg ccgcagctaa
     8341 ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc agagactgtc
     8401 gcgcaaagat gccgcttctg tgccggttcc tgccggaacg tgagttcccg tcaggcggct
     8461 tagctgcccg gcacgctcgg cggtaaagcc ggcattggca taagcgcaga tatcgcccag
     8521 cacagcccca gcctcaaccg aggccagttg atcacgatcg ccgagaaaga tcactcgcgc
     8581 atgatcgggc aaggcgtcga tcagtctcga catcataggc agatcgatca ttgacgcttc
     8641 atctaccacc agcacatcaa gatgcagcgg gttaccggca tgatgacgta aacgctggct
     8701 acccggctgc gcgcccagca atcggtgcaa agtgctggca tcttccggaa tgcgtctctt
     8761 ttgttcatcg gtcagcggta actgtcgcaa agccttgccg agagattcgg ttaagcgcgc
     8821 ggcagcttta cccgttggtg cagccagacg gatacggcag cgttcgccgt cggccatttg
     8881 aattaacgct gccagcaact tcgccacggt ggtcgtttta ccggtgcctg ggccgccgga
     8941 aatcaccgag atccgccgcg tcagcgccac tgctgccgcc actttttgcc agttaatttc
     9001 atcgcttact ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat caacctcaat
     9061 ggcatgattc acctcattga agaagcgcgc taccgtccgc tcgttacacc acatgcgatt
     9121 caaataaaga cgatcgccac agaggatcat cggcgtgggt tcatctcccc tgctgaccgc
     9181 ttgagaagcc agcaaggatt cttcccaatt ttgtagctca ccgatttcac tgacacaggt
     9241 cgccaacagc ggatgcgacg cctcgttatt ttccagtcgt gaaagcggca aacagacgtg
     9301 tccctctccg gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag gatgttcatc
     9361 tcccgccacg gtcagggcaa attgcacatc cagcgggcgt agctgtttgt gctccacagc
     9421 ttccagtaat tgcttttgca atttcattac gcctcctcca gggtcatacc ggcaaacatc
     9481 tcatccatca gggcaatcaa cccggcgttg ggtcgggttg tgtaaatccc ttgttgcgga
     9541 tgttctttat caacgccacg caggaacaga tagataacgc cgccaaagtg acgatcatag
     9601 tcgtaatcag caatgcgatg gcgcagatag cgatgcagcg ccagggtata aagctgatat
     9661 tgcagatcat agcggtgtgc ctgcatcgct gctgccatag cctgttgggt gtaagccgaa
     9721 ctgtcttcac ccagccagtt ggatttatag tcgagcaggt aataacgccc ttcgtggcgg
     9781 aataccaggt cgataaagcc ttttaacatg ccgcgtacct gcatgaactc cagcggcggg
     9841 cagccagcgg atagcgggtc aaactggcga attagtgcat caagctggct ggcgatgagc
     9901 ggttcgctaa tcggcaggta aaactccatc tccacctgtt tatcgcggtc ggaaagctga
     9961 ctcaggctaa cacccgtttc attgagaggt gcctggagga cagccgtgat ccactcggtc
    10021 aacaccggtt cccactgcga ttcaaagccg ccgagttcca gtttttcctg tacccagttc
    10081 gggtcaaccg gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa tgtccccggt
    10141 gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac gctgaccacg
    10201 cctgcggcat caacatccag ccgtggcatc agatcctgag cgataccgtg accacgctgc
    10261 tgcaagccgg agtagctggt gacacgccag ttatcgccgg gcaatcgttg tagtgtcctc
    10321 gcattcagtt ctgcagttaa agcatcatta acctgccagg gctggttatc accagtttgt
    10381 gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc
    10441 gcatcttgcg gttccccttt ttgcagcaaa cgcccgagcg cactttggtg gacgtcggtg
    10501 tcaccttttt tatcgccacg acggcgcacc agcggtgcaa cgccgagact gcaatgccaa
    10561 accgaacgcg tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg ttcgacctcc
    10621 gcgaggtcga cgctttctgg cgcagcatta agatccaaaa ctgcctcaaa cgagtggcga
    10681 tcgtgataaa acgcctgatc ctggacgcgg aaattggtaa taaatggcaa ccagaccagc
    10741 ggatattcca gccctttcga tttatggatc gtgacaatct gcaccagatg tttatcactt
    10801 tcgaggcgca tttgttggct ggaggcatta ctgtctggct cgaggatatg ttgcgataac
    10861 cagcgtacca gcgcatgttc actttccagc tgcgttccgg cttcttgaag cagttcgctg
    10921 atatgcaaga tatcggtaag acgccgctca ccgcctgccg ttgccagcaa gttttctgca
    10981 atgttacgcg ccgacatcag cgcccgcagc atcggcataa cgccacgttt gcgccagatt
    11041 tgccgataac catcgaactc ttcgactacc gcatcccacg catgttcgtc attattcagc
    11101 gtttcaatat ccagcgcatt cagccccatc attgacgttg ccagcgcact gcgcagggtg
    11161 ttctcacgtt cgggcgtcat caccgcctgc aacagccaaa gcatttcctg cgcttccaga
    11221 gtttcaaaaa cactatcgcg gttcgaaagg taaacggaag ggatttccag caacgttaag
    11281 gcatcgcgca cctgggcggc ctcctggcgg ctgcgcacca gcacgctgat gtccgaagca
    11341 cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg tccggcttgt
    11401 agccagtcgc ggatttgcgc agcacatacc tgcgccatgg tactttgata gtcgccaacg
    11461 ccgcagcttt cgccttccat cagccacatt ttcatcgcag gctgcgtttc acctttaaag
    11521 acaaaacgta acgcctgatt tttcccggct gatttcactg gaataaacgg tatttcgcga
    11581 aacatgaacg cgtcatcagt ctggctgaaa agcttgttca cactgttcac cattccaggt
    11641 gcggagcgcc agttggtgtc taaagtgtag tgggcgtgaa cttcactacg cgccttcata
    11701 taagtgaaga tatccgcacc ccggaatgca tatatggcct gcttcgggtc gccaatcagc
    11761 aacaatgcgg tttctggctg atggtgccag atacggcgaa aaattcggta ctgctggggg
    11821 tcggtatcct gaaattcatc gatcattgcc accgggaatc gcgtacggat cgccgctgcc
    11881 aacacctcgc cgctttcgct acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa
    11941 cccaattcgc cacggcggcg tttttcacgc gctactgttt cgcggatctc agccaatgcg
    12001 cgggtgatca ccagatcacg gatcgacaat ggttctgcaa gcagttgatc gatcgcctca
    12061 aacagtggat gtcgcggggt ttcccccccg gctttcgtgc gatcttctaa gaaacgctgg
    12121 gagaattttt ccagcgactc cggcaactga taactgtttg tctcttcttc tgcccaggcg
    12181 ctgatcttgt cgatccattt agcctgattg ctacggttaa acttgcgtcg atcaatacca
    12241 gaagattcga tcagcgcatc cagttcaccc actgcgtcgc gccactgctg ttttaccgta
    12301 tcaatacgcg ccacaatttg cgcgtgacgg gaagctagcg tttcatcatc gggcggcggt
    12361 gctttgataa ccggcgcttc gccttgcaga taacgattaa tatcgcgcag caacgcctgc
    12421 ggccctttcc aggtttcaaa gacgacctgg gctatttcac gcggcagcgg gtagcagtgg
    12481 cgacgccaga aatcggcgca ggcctggtag cgtagcagag actcatcttc aatcagctgc
    12541 tgctcaaaca gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg gcaaaagccg
    12601 tgaatagtaa agactgccgc ttcatccatc tgccgttcgg ctaacaacaa ccactgcgcg
    12661 gcttgcgctt tatcgtcgac ctcttccagc aggtgttcgt acaatggatt gtcggtggtt
    12721 tcacgcagac aggcgatgcg caactcgtgg atattgctac ggatacgacc gcgcaattct
    12781 gccgtggcag cctcggtaaa ggtgaccacc agcagttctt caacggtcag cgggcgggga
    12841 aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat aaagcgccgc aatcgtaaag
    12901 gttttgcctg tgccggcaga ggcttcaatc aggcgctcgc cctgtaaggg caagcgcaaa
    12961 ggatctagtg tctcggcgac atcactcatt cttttcactc atcaggggca ttgtttgctg
    13021 caacgcgctg acgttctccc acactttcca gccttcagga tgcacatatt cggctttccc
    13081 gttttggctg ccggaaatct gcgacaggat agccatgcct tgcggctcga ctaccgcctg
    13141 atggaagaaa tcggccagtt tttgcggcgt cagcagttta atctgggcca cgattttatc
    13201 acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta cttaacttcg atgcttcttc
    13261 gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga tttgcgcaaa
    13321 ctcatctggc ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg ccttgtaacg
    13381 ctcccacaag aatgaaggct gtttatcatt gctttgcaaa aggaagccca tgccccactg
    13441 acgccccacg ctcattggaa acgcaaacac ggcatagccc aattgttctt cggtacgcaa
    13501 ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg ctgagctggt
    13561 gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg tgctgttacc
    13621 ggctttttca aagatgacgg attgtttttt atcgaccact acatctttgt ttcgacacca
    13681 ctctgaacca tcagcgccca actgtttttg cacatcgcgt gccagcgttg ttgcctgggc
    13741 ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cctgatttta aggcgtcgcg
    13801 ataagccagc acctctttca acgtaatgga gggcaaaatt ttacgccgtt catctcgcga
    13861 gaagtacggc acttgcgaga gcatctgcgc gggcataatc gcctgctcaa acgctttacc
    13921 cttttctgcg gaatccatca tctggttata ccaggacttc gcctgctcaa gctgatcttc
    13981 cgtagcggta tagctaaagt acccctcgag caatgcctgg aacagctgcg gcagacgctg
    14041 ggtgtaacca ttagcattaa ccataaggcc gttgttagcg ttggtggaaa aacttatgcc
    14101 accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat aatcattgag
    14161 cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca aaatcaggct
    14221 gacatcagct ttgggttcgc tggaaaaata acggcttggc gcatacacca cgcgcagatt
    14281 cgactcatca acaatcagct ctggatggtc atatttcttc tctgacttaa tcagcgagaa
    14341 atcgtcagga atataggggt taagctccgg taaggagagc gcaatattgg cagctttttg
    14401 ctgccagtca gcgaaagttt gttcgctgat tttatcgacc tgatacggcg catcgacaaa
    14461 ataagccgtt ttattgtgcg gctctttcgg gctgatatac cagatacgcg cattctgcgg
    14521 cgtcatcatc gccagacgtt cttttactgc tttagcatcg taccgatcgg caatattgac
    14581 tgcatccagc gtatgctcaa ccggaacgcg aatcatggta tctgccagcc attcgacgta
    14641 atccatatca cgggtgatcg acggataacg gaagtcgata tccagcacat tcgccagttc
    14701 atcgaagtat tgtttatcaa tgcctttttc acgtaacaga ttgagatagc taaaaattgc
    14761 cgccacaacc tgatcgcgat tagccaggcc tttatcggtt aaagacgcag agatcgctaa
    14821 tacgccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct caactaatcc
    14881 ctgcttttgc agccagtcgg aaagtgtacc tgggctgcga ttaccaatca gataggtaat
    14941 caattcatcc gttttactac ggaacttcgc tgagttgtta tcgatgcgaa actcaacgcg
    15001 cagaacttta cgcggcattg ccgggacgta atgaatgata atgccctttt gcgcgtcggt
    15061 gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc gaccaaaggt
    15121 gtccgccgcc atttttgcca actccggcag cggtttattg ctgtaaataa ccgccttcat
    15181 cagattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca ccggattacc
    15241 aggtttatcg cttaaagttt cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg
    15301 gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac gcgccatggt
    15361 taattcagcg ttcaccgcat tacgctcacg ttcggcatat ttcttgtcga gcaaaggttc
    15421 agcaatagca tcggccaggc ggtctaccgc accaggcaag gcgtcgttct caacttccag
    15481 atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt gcattttgag
    15541 atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac tcatatgttc
    15601 aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa cgggcaccac
    15661 cagcgccgag agcgatttaa ctgcctgcgg atcagaaacc agcaagacca ccataccgtt
    15721 atccagacgt atagcctgat actggcggtt atctttatca cttttacgga tggtttcctg
    15781 aatcggctgc catcccgttt ctgcctgact taagggtgcc caaagggcaa ctaacaacaa
    15841 taatgctttg aaccaggtgc tgcggggcat tctcggacct cataatcaac ttaattttct
    15901 gtccagattc aacacgttaa cgcaaatcat ctgccagaat ttaatcttgt gctgcacgag
    15961 tcagcctatg tttatataac catcagtccg tgactggtgc gcatcataaa gtaagcggat
    16021 agattgcgca atttttatac agcactcatg actgattaaa gcgaaacagc ggtaacagga
    16081 aacgttgcga ctgttcaacg atggcctcca ttgtctctgg tgttaattgc cgccagagcc
    16141 tttgatacca gatatcatca ccttcgccac gcaccatcat gttaccttcg taagcctgaa
    16201 ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca tcgttttgcg
    16261 cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc aatggcgcgg
    16321 acatcccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg
    16381 caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt tcaccattac
    16441 caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgc gccacactta
    16501 ataaagaggg acgccagcgc aacaggccat ccggctgcac ctgcggcaac cagccagtta
    16561 tctgcacacc gttgcaggcg agatcaattt ccatactctg ccccggctgg cgacaggcaa
    16621 tgactctgtc ggcaagctgc tgcatctcct ggcactgagt ttcccagaaa atttcaccaa
    16681 aagcgccata cggtaaatcc cctgccgccc ggaagcgacg gaacaagcgt tcggcatcat
    16741 cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta agcccttcca
    16801 gaataaatgg ctcggtgtcg gggatttcgc tgtcttcagt acggaagttc acctgcaaac
    16861 gcatctggaa gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt tccagcggca
    16921 cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct ttaccagcct
    16981 ggctggccgc aggtagccat tcacgagcat agctttgtcg ttcgcctggc tgatagtttt
    17041 gtggatcaaa cggcattcgg gtatggaggc aagtaagatg cgcttttacc cttgcctcgc
    17101 tttcatcaca gttgagcgct tcatcgcccg gtagataatg actttgcccg atgtagtcga
    17161 tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg gaacgcccga
    17221 tatagctgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac agatagcggt
    17281 cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg tcaaagccca
    17341 atggcgcaag ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa accactttga
    17401 acggaattga acgcattggc atcagagtac aaatgttaac cggtccggcg agaaaacgct
    17461 ggctgatacg ttcttgatcc agacgctgtg ccagttcatc acgcaatagt gacagcggca
    17521 ccgcgtcgcc atactgcgca cctaaacctt cggcgataat cgcctgccat tgttgttcga
    17581 tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gaaggcgctg agcatatcgc
    17641 gacaaaccgg caaccactct tctagcggac gctcctgtgc cagcccgcga cgccagatgt
    17701 ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag ccgctcgatt
    17761 catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg tagcccagca
    17821 acatacgcgt caggccaaat cgccaggtgt gttgtccggt ggcggggagt tccagctcgc
    17881 gaacgttgtc gtcatctatc ccccaacgaa tgccggattc gttgacccac tggcgtaaat
    17941 aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca tccagtaatg
    18001 ccagcacgtc ttccgacaca aagcggctgt ctggcaacga taacaggctg ataaacgctt
    18061 ccagtaccgg atgcgactgc cgcgcccgac ggtcggaaat ggcgtaaggc aggtaacgat
    18121 ccgcaggtgc gctaccaaac acagcccgaa taaacggact gtagctgtcg atatcagcca
    18181 ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc ttccagcatc gccagcaggc
    18241 gatcgtgcaa aacttcaact tcacgctgcg ggctatggca aacgtggaag gtgatactgc
    18301 tatccagtgg atcaagcggg cgtttgttat cgctacggga aaactcttcg atgttcacac
    18361 cagcaacggc acggttttcc agttccagaa tgtcagactg aatgttatgc agcaggttat
    18421 ctggcgtgac atcgacaaag gcgtccagct cctggctgct ctccaggtca gaaaggagat
    18481 aaatgtagtc gcgcccaagc ttaccccatg aagccagcag cgggttgccg acatcctgtt
    18541 caccatcgct gttaaagagc tgcccggcat tttcgctatc acgaaatagc ggcaattcgc
    18601 gatcttcaaa actgtgtcga cgctgacggg tcagcagttt cgccagataa gcaggatctt
    18661 taatatcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt tcaatatgtt
    18721 tacccagcgc ctgtagcgcc tggagataaa caggcggtaa cgcggaaata ccgcatataa
    18781 agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc
    18841 gctgatagag attggcgcgg tgccagcgcg gttgcccgag ttgatgggta tattccacca
    18901 gcgccttcca caacggggct tgccaggcct gtgcttctcc cagcccttca accaaatgtc
    18961 ctgtttccca ctgtgccagc cagtccggac gatagaccag atactggtca aacaggtccg
    19021 ccgcttttga ggaaagctgg aacagttttc gcttgtcgct atcgtcagtc agataatgcc
    19081 gcaacagggt aaagtcttcg cgctccagca attgcggcag cagagtcatc agtttccagc
    19141 tcatgctctg tttgttaaag gcgctctctt tggggatttc cggtaacacc cggacgaaca
    19201 tatcccagat aaagctcgct ggcagcggaa aatcaatgtt tgccgcaata ctaaactttt
    19261 gcgacagggt catttgcagc cactgcgcca taccggtact ttgcaccaga atcatctctg
    19321 gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac gcttccagca
    19381 cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac tgacgattcg
    19441 ggcaatgcag gcgggtcatc tcgccttctc tgcccccggg tgaaactagc gtaacgctga
    19501 tgctgacaca tcccgcctgc gatgtctgca ttcggttgac ctgccagttg gcaggtggcg
    19561 aaatcgcgcg cagttgcgtt tgctgccaac catgccgcca gagctgttgg tactggtttc
    19621 tgctggcgag actgttcatt aatgttcgct ggatacccga taacgcagtg acaatcatca
    19681 ccatcagcac catcgcaacc attacctccg gcaggctaaa gccttgttga ttcttcaggg
    19741 aagctgacat aacgccctct ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa
    19801 aacaatgttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt cataaccggc
    19861 aaccaataag gcttcattat ctgccagtaa acgcaaacaa acccgggcat cggttccagc
    19921 gtactgcgag cactgaactg cgggctgcgt ctgccaggag tgcattttcc cccacgccag
    19981 cgccgactga acgatggcct ggcggcgcaa tgactgactt tctatgctca cgcgagaggc
    20041 aaaactgcga tcctgctgac tcattccttg caatagcaag ctacccaaaa ccagcagcat
    20101 caggaccaga gccagtgacg aaacaccttt ttcgcggttc acaggttgaa tcctgtcacg
    20161 ctatagctgg catccaccac ggtttgcggt tcagacttac tggcagcacg catattaacc
    20221 gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt gtcgataatg
    20281 atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt cgcaccgcgt
    20341 agcgtttcca gcacatgctc cttcagacga aatccaatct ggtcagactc ttttaccggt
    20401 tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca gtcaccctgt
    20461 ccgacaattt ccagcccttc gccggtacag ctgccatgac aataacccgc cctctggaga
    20521 tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagcgccag cttacgggtg
    20581 ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg cagcccccag caacaatacg
    20641 ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc ttttacaggc
    20701 atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa accaccagcc
    20761 accactcgcc cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc
    20821 caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa
    20881 agaccaatgg tgaactgcca tgacaggtat tggccccagc agcggaactc acaaggcacc
    20941 ataacgtccc ctccctgata acgctgatac tgtggtcgcg gttatgccag ttggcatctt
    21001 cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccatagc cgttgcgact
    21061 gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa atcagcatcg
    21121 cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca gtatggagcg
    21181 aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg gactatttac
    21241 gccgttgcag gacgttgcaa aatttcggga aggcgtctcg aagaatttaa cggagggtaa
    21301 aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta gatagccacc
    21361 ggcgctttaa tgcccggatg tggatcgtag ccttcaatct caaagtcttc gaaacggtag
    21421 tcgaagatgg attcgggttt acgtttgata atcaacttcg gcagcggacg cggttcgcgg
    21481 cttaattgca gatgagtttg atccatatga ttgctgtaca ggtgcgtatc gccaccggtc
    21541 cagacaaaat cacccacttc cagatcgcac tgctgcgcca tcatatgcac cagtaacgcg
    21601 tagctggcaa tgttgaacgg caggccgagg aagacgtcac aggagcgctg ataaagctgg
    21661 caagagagtt tgccgtctgc cacatagaac tggaagaatg catggcacgg tgccagcgcc
    21721 attttatcca gttcgcctac gttccacgct gaaacaataa tgcggcggga atccgggtcg
    21781 tttttcagct ggttcagtac cgtagtgatc tggtcaatat gacgaccatc tggcgttggc
    21841 caggcgcgcc actgtttacc atacactggt ccgaggtcgc cgttttcatc ggcccattcg
    21901 tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc ctgcagaaac
    21961 cacagcagtt cgtggatgat ggaacgcagg tggcaacgtt tagttgtcac cagcgggaat
    22021 ccatcctgca ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc ggttccggta
    22081 cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa atactgtttc
    22141 atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac catcatgatc
    22201 acacccgcga caatcatcgg gatggaaaga atttgcccca tgctgatgta ctgcacccag
    22261 gcaccggtaa actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg
    22321 tagccaatca ggaacaagcc agagacagct cccattgggc gcggtttacg aatatacagg
    22381 ttgaggataa taaacagcac cacaccttcc agcagcagct cgtaaagctg tgatgggtgg
    22441 cgcggcagca caccgtaagt gtcgaaaatg gattgccact gcgggttggt ttgcagcagc
    22501 aaaatatctt ctgtacggga gcctgggaac agcatggcaa acgggaagtt cgggtcaacg
    22561 cggccccaca attcaccgtt aataaagttg cccagacgcc cggcaccaag accaaacgga
    22621 atgagtggtg cgataaaatc agaaacctgg aagaaggaac gtttagtacg gcgggcaaag
    22681 ataatcatca ccacgataac gccaatcagg ccgccgtgga aagacatgcc gccgtcccag
    22741 acacggaaca gatacagcgg atcggccata aactgcggga aattgtagaa cagaacataa
    22801 ccaatacgtc ccccgaggaa gacgccgagg aagcccgcat agagtaagtt ttcaacttca
    22861 tttttggtcc agccgctgcc cggacgattc gcccgtcgtg ttgccagcca cattgcaaaa
    22921 atgaaaccca ccagatacat caggccgtac cagtgaagcg ccacgggtcc tattgagaaa
    22981 atgaccggat caaactccgg aaaatgcaga tagctactgg tcatctgtca ccacaagttc
    23041 ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt caggcgctcc
    23101 aaaggtgcga ataatagcac aaggggacct ggctggttgc cggataccgt taaaagatat
    23161 gtatatgatc cgcgctataa ccctccgcga atcagcccgc ccatgccgcg acgctccata
    23221 aaggctgcaa cctgatggcg aacttcggtc gccagttgcg cttccagact acgctgcgca
    23281 agattttctg cttcggcata atcaatgcgc cgcagcaggt attttacccg cgctacagaa
    23341 cgtccgttca tagacagatg gcgataccca agcccaatga ggattgccac gcacatggga
    23401 tcgcccgcca tttcaccgca caaacggaga tcgattccat gtatttccgc ttcccgggcg
    23461 atcatcgcca gagctcgtaa cattgcagga tgaagactgt cataaatgtt cgccacccgg
    23521 gtattgttgc gatcaacggc cagaatgtat tgagtcagat cgttggtgcc aacagagatg
    23581 aaatcgaccc gctttgccag atgcggcagc ataaatacca ttgacggcac ttccagcatg
    23641 atgccgatac gtggtttggg aatttcgtaa ccgatcatct cctcgacttc acgtccggca
    23701 cgttcaatca ggcggcgtgc ttcatcaact tcatcgaggc ttgtgaccat cggcaacaga
    23761 atattcaggt tgcccgtagc ggcattagca cgcagcatcg cccgcacctg gatcaagaag
    23821 atctccggct gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg attctcttcg
    23881 ctgatcggca tgtaaggcag ctgcttatct gctccgacat ccagcgtacg caaggtgacg
    23941 ggtttatcat tgaacatttg cagcatcccc tgatactgcg ccacctgttc ttcttccgac
    24001 gggaaaccac tttgcagcat gaatgggatt tcagtgcgat aaagaccgat gccatcaata
    24061 cggctgccca gtttttcttc atgttccggg cttaaaccag cattgagcat gacttttata
    24121 cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat cttccgccag acggctaagc
    24181 tcaatctctt cactaattag ccgctgatat tcttgcagca gtaccggctc cggatcgacc
    24241 agcaattcac cgcgatagcc atcaacgatc agcgtcctac gatgcagcac cgaaggctga
    24301 atatccgcgc ccatcacggt agggatcccc agcgcacgta ccatgatcgc agcatgggag
    24361 ttggctgcgc catctcgcac gacaacaccg actaagcgat cctggggcag ctcagcaaga
    24421 gttgtcgctg acagttcatc tgccaccaga atgaaacgtt ccggccaggc gttcggccct
    24481 tgattagcgt catcaagatg aaacagcaat cgctgaccca gcgcacgtaa atcgccagcc
    24541 cgctctttga gatagttatc gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc
    24601 gtttttaccg cccactctgc caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc
    24661 agccgggtgt ccgaaagtag gtgcgagtaa agatcgaaaa tagccgccgt ttctttttgt
    24721 gctccggcgg caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc ttccagcgcc
    24781 ccggtcagtc gttcgcgttc cagagccgga tccagcgttg atgcctgata cacctgttcc
    24841 attaaaggta acgtggcatc ctgccagcct tcggcaatcg ccacaccagg tgctgccggt
    24901 aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaagg cagtcaactg cgactgagaa
    24961 agaatagctg ccatctgggt ggcaagcgtc accaggaagg attcttcact ttcgtcatac
    25021 tggcgcaact ctcgttgctg taccaccagt acaccaagca actggcgacg ttgaataatt
    25081 ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg aggggatgta tttgaagctg
    25141 gggtgctttt gcgcatctgc aaggtttatc ggttccgcca gcctgccaac caggccgacg
    25201 atcccttcat caaacgcgag cgttacagtg cgaccgcgtg gttttttcag cccccgggtc
    25261 gccatcaggt agtaacaacg tcgatcatga tcggccaggt agaccgaaca gacctcggta
    25321 tccatcgcaa gacagatgtc ggtaaccaga atatttaacg cctcattcag gcgtggtgcg
    25381 ctggctacct tttcgactat ttcgcgcagg cgagtgagca taattggcgt gacttaacct
    25441 cttttacgtc gataagcaga tgtgttttgt ggttttggcg tattttcctg cagtgacatc
    25501 accacactcg cgaactcttt cattacccta cggtagacat cacgtttaaa tgacaccacc
    25561 tgtctgaccg gataccagta acttacccat cgccagccgt caaactctgg tgtactgctg
    25621 gtttgcatat tgatttctgc atcgccgctc accagctgca agagaaacca tttttgtttt
    25681 tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc
    25741 aaccagttac gcgttgaagc aaggattcga acgtctttgc ggcttaatcc tacttcttca
    25801 aacaattcac ggtacatcgc ctgctctgcg gattctccgg ggttgattcc gccttgcgga
    25861 aattgccagg agtgctgacc aaatcgccgg gcccacatta cctgcccctg gcgattacaa
    25921 atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact acctcaaaat
    25981 aaagctttat atacgaatga ttgtttcata ctccaagaag acggtaaacc actctctgca
    26041 gggcattaca cactaataac aattgaataa ctcacagtta tgtgcagagt tataaacaga
    26101 ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt gaagaactac
    26161 ggaattacta cgggaaaacc cggagcattc tgaataagcc cgatatagac attttaaaat
    26221 attcattatt agtcaatgca ttacatgtaa tttgagtaat gaaaaagagt aattcgtgac
    26281 ccaggtcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt atttatccac
    26341 agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt cgcacgtcaa
    26401 ggctgtaaat ggaaacagta gtggaggttt ttcacagtta tcccagcttt ctgtggataa
    26461 catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacctat tgcagtgttg
    26521 cgcaactcga ttaccggcag cctaaaaagc aatataaatc agagaattga acaacacatg
    26581 tggaaaaagt tacactgcga atattcggca cataattgct gtttgttttt taaccaaggt
    26641 atcatgacat gtcccaacct cgcccactcc tctctcctcc cgaaactgaa gaacagttgt
    26701 tagcgcaagc acagcaactt tctggttata cattgggaga actggcggca cttgccgggc
    26761 tggttacgcc agagaattta aaacgcgata agggctggat tggcgtgtta ctggagatct
    26821 ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgctgctctg ggcgtggaac
    26881 ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc gtttgtgttg
    26941 ccccgttaac gggtaatagc ggggtgacct gggaaaccag ccatgtgcgc cacaagctca
    27001 aacgcgtact gtggataccg gttgaaggtg agcgcagcat cccgctggcg cagcgtcgcg
    27061 taggatcacc gttgctgtgg agcccgaatg aagaggaaga ccgacagcta cgcgaagact
    27121 gggaagaatt aatggatatg attgttctcg gtcaggttga gcggatcacc gctcgtcacg
    27181 gggagtattt acagatacga ccgaaagcag cgaatgcgaa agcgcttacc gaagccattg
    27241 gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt ttatttgaag aagaatttca
    27301 ccagtgcact actggcccgt cattttctga tccagtagcc atcgctttga cctgccgctt
    27361 tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt tatttgcatg
    27421 gataaccgat cctaacgcct ggcttgcgct cggtacgctg acgctgctgg agatcgttct
    27481 tgggatcgac aatattattt tcctttctct ggtggtggca aagctcccca cagcacaacg
    27541 tgctcatgcg cgccgtctgg ggttggcggg agccatggtt atgcgtctgg cgctgctggc
    27601 atcaatcgcc tgggttacgc gcctgacaaa tccgcttttt acaatattca gtcaggaaat
    27661 ttccgcccgt gatttgattc tgcttctggg tggcttgttc cttatctgga aagccagcaa
    27721 ggaaatccac gaatccattg aaggtgaaga agaagggctg aaaacacgcg tttcatcatt
    27781 cctcggcgct atcgtgcaga ttatgctgct ggatattatc ttcagcctcg actcggtgat
    27841 taccgctgtg ggtctgtcag atcacctgtt tattatgatg gccgccgtgg tgattgccgt
    27901 aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc atccttcggt
    27961 aaaaatgctg gcgctctctt tcctgattct ggtcggcttt accctgattc tggaaagttt
    28021 cgacatccac gtaccgaaag gttacatcta cttcgcgatg ttcttctcta ttgcggttga
    28081 aagcctcaac ctgattcgca acaaaaagaa cccgctctga tactccgtac gctctcctgc
    28141 gggagggcgt actcactcct gttaccctca cagtttaaga ttttcctgct ttcaagatat
    28201 ataacgtcgg tttataaaca gactattatc ataggtgggc caaatgagga taaccggatg
    28261 aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc cgggtgttcc
    28321 agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg aaaacctgaa
    28381 attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc gatgcaaatt
    28441 aaccgtgatg atgtttcgca aattattgaa cgttaacaaa taaggtcagc atccggctgt
    28501 aatgccgatc agttaag
//
LOCUS       DAZOYL010000017        27737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZOYL010000017 DAZOYL010000000
VERSION     DAZOYL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.017"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(156..1067)
                     /gene="gsiD"
                     /locus_tag="ACHUOY_000546"
     CDS             complement(156..1067)
                     /gene="gsiD"
                     /locus_tag="ACHUOY_000546"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR0681430.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1070..1990)
                     /gene="gsiC"
                     /locus_tag="ACHUOY_000547"
     CDS             complement(1070..1990)
                     /gene="gsiC"
                     /locus_tag="ACHUOY_000547"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR0681431.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2008..3546)
                     /gene="gsiB"
                     /locus_tag="ACHUOY_000548"
     CDS             complement(2008..3546)
                     /gene="gsiB"
                     /locus_tag="ACHUOY_000548"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR0681432.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3566..5437)
                     /gene="gsiA"
                     /locus_tag="ACHUOY_000549"
     CDS             complement(3566..5437)
                     /gene="gsiA"
                     /locus_tag="ACHUOY_000549"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR0681433.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5424..6389)
                     /gene="iaaA"
                     /locus_tag="ACHUOY_000550"
     CDS             complement(5424..6389)
                     /gene="iaaA"
                     /locus_tag="ACHUOY_000550"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0681434.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6593..7828
                     /gene="moeA"
                     /locus_tag="ACHUOY_000551"
     CDS             6593..7828
                     /gene="moeA"
                     /locus_tag="ACHUOY_000551"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR0681435.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7828..8577
                     /gene="moeB"
                     /locus_tag="ACHUOY_000552"
     CDS             7828..8577
                     /gene="moeB"
                     /locus_tag="ACHUOY_000552"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR0681436.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8756..9418)
                     /gene="fsa"
                     /locus_tag="ACHUOY_000553"
     CDS             complement(8756..9418)
                     /gene="fsa"
                     /locus_tag="ACHUOY_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0681437.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9549..10448
                     /locus_tag="ACHUOY_000554"
     CDS             9549..10448
                     /locus_tag="ACHUOY_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR0681438.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10454..12886
                     /locus_tag="ACHUOY_000555"
     CDS             10454..12886
                     /locus_tag="ACHUOY_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR0681439.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13032..13847
                     /gene="ybiV"
                     /locus_tag="ACHUOY_000556"
     CDS             13032..13847
                     /gene="ybiV"
                     /locus_tag="ACHUOY_000556"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR0681440.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13999..15264
                     /locus_tag="ACHUOY_000557"
     CDS             13999..15264
                     /locus_tag="ACHUOY_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR0681441.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15231..16391)
                     /locus_tag="ACHUOY_000558"
     CDS             complement(15231..16391)
                     /locus_tag="ACHUOY_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0681442.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            16629..17930
                     /locus_tag="ACHUOY_000559"
     CDS             16629..17930
                     /locus_tag="ACHUOY_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0681443.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            17945..20308
                     /locus_tag="ACHUOY_000560"
     CDS             17945..20308
                     /locus_tag="ACHUOY_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR0681444.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20488..22083)
                     /locus_tag="ACHUOY_000561"
     CDS             complement(20488..22083)
                     /locus_tag="ACHUOY_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR0681445.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22302..23222
                     /gene="ldtB"
                     /locus_tag="ACHUOY_000562"
     CDS             22302..23222
                     /gene="ldtB"
                     /locus_tag="ACHUOY_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0681446.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23281..24399)
                     /locus_tag="ACHUOY_000563"
     CDS             complement(23281..24399)
                     /locus_tag="ACHUOY_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR0681447.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24396..24863)
                     /gene="mntR"
                     /locus_tag="ACHUOY_000564"
     CDS             complement(24396..24863)
                     /gene="mntR"
                     /locus_tag="ACHUOY_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR0681448.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25049..25177
                     /gene="mntS"
                     /locus_tag="ACHUOY_000565"
     CDS             25049..25177
                     /gene="mntS"
                     /locus_tag="ACHUOY_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR0681449.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25449..27032
                     /gene="opgE"
                     /locus_tag="ACHUOY_000566"
     CDS             25449..27032
                     /gene="opgE"
                     /locus_tag="ACHUOY_000566"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR0681450.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27081..27596)
                     /gene="ompX"
                     /locus_tag="ACHUOY_000567"
     CDS             complement(27081..27596)
                     /gene="ompX"
                     /locus_tag="ACHUOY_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR0681451.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27649..27714)
                     /locus_tag="ACHUOY_000568"
     CDS             complement(27649..27714)
                     /locus_tag="ACHUOY_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681452.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
BASE COUNT         6720 a         7673 c         6990 g         6354 t
ORIGIN      
        1 ctatctgaac gctgtgccgc tgcaaaaata gtcccttgct gcggcttttt aaccaaatta
       61 cccgattcta caaaatcagc agaaagttac aacacttgct tagggcaata atttcctgtg
      121 aggtgattac cctttcaagc aatattcaaa cgtaactatc ctttaatttt cggatccagc
      181 gcatcgcgta aaccatcgcc caacaaattg aacgccagta cggtcagaaa aatagccagg
      241 gccggaaaaa cagcgacatg cggcgcgata accatatccg ctcgtgcctc attgagcatt
      301 gctccccact ctggtgtcgg cggctgcgca ccgaggccga gaaatgacag gctggcggca
      361 gagataatcg aggtgccaat gcgcatggtg aaaaacacca cgatagacga gacggtccca
      421 ggcaagatat gacgcaataa aatggtcata tcgctggcac caatactgcg tgctgactca
      481 ataaaggttt gctgtttcaa caccagcgtg ttgccgcgca ccagtcgggc aaacgcgggg
      541 atggaaaaaa tggcgactgc gataatcacg ttagcaatgc cgcttcccaa caccgcaaca
      601 acagcgatcg ccagtaaaat acccgggaag gcaaacagca catcgcaaat gcgcatgatc
      661 agccgatccc accagccttc ataatatcca gccaacaagc ccagcaacgt cccgatcgcc
      721 gcaccgataa acacggcaaa cacgcccgcc gccagcgaga tttgcgcacc aaccaggaca
      781 cggctgaaaa tgtcgcgccc cagtgaatcg acgccaaacc agtgctgcaa agaaggtccg
      841 ttattcagat tgtcataatc aaaataattt tcggcgtcat agggagcgat ccagcgtgca
      901 aaaatggcca ccacaatcaa taaaataacg aataatgcgg cggtcatcgc catatgctga
      961 cggcgaaatc gtcgccagaa ttcatgccac ggtgtacgca cctggtcagg tttgaccagt
     1021 ggcatggcgt ttaacaccgc ctgacgtcgc cagttaaata gtcgcatcct tacttgtacc
     1081 tgatagccgg gttaatggcg gcgtaaagca catccaccac taagttgata agaataaatt
     1141 ccagcgagaa aagcagaatt tccgcctgaa tcaccgggta atcgcgcatt tctacggagt
     1201 caacgagtaa gcgtccaagt ccgggccagt tgaaaacttt ctccaccacg atagaaccac
     1261 cgagcaaaaa gccaaactgt aagcccatca tggtcactac cgggatcatc gcgttacgta
     1321 gcccgtgttt gaggacaacc caggtttcgc tcaccccttt cgccctcgcg gtacgcatat
     1381 aatcttcgct taaaacatcg acaaacgacg cgcgggtaaa gcgcgccatc acggctgcca
     1441 ctgccgcgcc gagcgtcagg gagggtaaaa tgtagtgctg ccaactgtct gctcccacgg
     1501 taggcagcca gcccagttca acggagaata cctgaattaa aagcatcccc agcgcaaatg
     1561 ccggaaacga gatccccgac accgcaatgg tcatactcaa tcgatccggc caacgattac
     1621 gccagacggc ggcgataatt cccgccgcca taccaaatat caccgcccag accatactgg
     1681 ttatggtcag ccacagcgtt ggcataaagc ggctggcaat ctcatcggca accggacgac
     1741 gcgacaccat cgacaggcca aaatccccct gcacagcatt gctgatatag tgccagaact
     1801 ggtgatacag cggctgatcc aaccccagct gctgacgcac cagttctata acctgcgcat
     1861 cagcttcgag cccggcaatc aatcgcgccg gatcgccggg cagcatatgc acaaataaaa
     1921 acaccagcac cgagacgata aacagcgtcg gaatcaaccc cagtaagcgt ttgataacgt
     1981 aattaagcat tccactccct gcgttgctta ttgcaaatcc gcgtcttcaa agctgaagcc
     2041 ggtgtctggc atgatccaaa aaccggtcag gtttttactg tgtgccgaca ccagtttttc
     2101 taccaccagc gggatccacg gcgactcttg ccagatgata tcctgcgccg ccttatacaa
     2161 gcgggtcttt tccgccggat cattagtttt cagtgcctga gccaggaagt cgtccacctg
     2221 tttattgctg taaaatgcgg tattaaacag cgtcggtggc cagttttgcg aggcgaacag
     2281 tggcgatagc gcccagtccg cttcgccggt tgaagccgac cagccagtgt agaacatccg
     2341 cacgccgctc tctttttgcc ctttaccttc aacttctgcc gcccgctgtc cggcatccat
     2401 cgcggtcacc tgggctttaa tcccgacctg cgctaactgc tgctgggtaa attgcagcac
     2461 tttctgcgcg gtgctgtggt tatgtgacga ccacagcgtg gtactgaaac cgttgggata
     2521 tcccgcctct ttcagtaatt cgcgcgcttt cactggatcg taaggccacg gtttataact
     2581 ttgcgcgtag gcgatacttg gcggtaccac accagtagct ggcgttgcat agcccgcaaa
     2641 ggcaactttc accagcgccg gacggttaat ggcgtaattc agcgcctcac ggaccttcgg
     2701 gttatcgaac ggcttttgcg tcacgttcat actgatataa cgctgcataa ttgacggact
     2761 ggccatcaac tcgatatttt tgtttttctc cagcagtgtg gcctgctcgt agggaatggg
     2821 gaaagcaaac tgcgcttcac cggtttgcag cattgccgcg cgggtgttgt tatccgccac
     2881 cggacgccag gttatgctgt ccagtttggg caatcctggc tgccagtaac ccgcgaattt
     2941 tttcaccttc acaaaatcgg tctgattcca ggtatccagt tcatacggtc cggttcccac
     3001 cggatgaaaa ccaatctcct tgccatattt ttccagcgct gccggtgaaa tcatcgcggt
     3061 cgccggatgg gcaagaatat taataaacgc tgagaacggc tgtttgaggg taatctttac
     3121 cgttgtcgga tcgatcgcct ccgttttggc aatattctta tacaggttat agcgtttaag
     3181 atgattcgcc ggatcgctgg cccggtccag attcgctttt accgccactg cgttgaaatc
     3241 ggtgccatcc tggaatttaa tgccttcccg caatttcacg gtgtaagtaa tgccgtcatc
     3301 ggaaacggta taactctccg ccagcacgtt tttcagtttc atctctttat ccagaccgaa
     3361 cagcccctgg taaaacgatt tcgctacggc ctgagataac gtatcattcg cgtcatacgg
     3421 atcgagcgtg gtgaaattcg atcctaccgc caccaccaca tctttggcag cgaatgcaca
     3481 agatgccatc aacgctgtcg caatgcccag cgccactaac ccactacggt gtacagctct
     3541 tgccatttta ttctccgcct gaatgttatc tacgcatgaa tgcgtattct gattgtggtt
     3601 gtgcgacgta atgccccggc ccgacgcatt gcaacgagac ggctgccacc tcttcgccac
     3661 gcagatgaat attgctggga agatcgtccg acagcagtac acgctgcggt cgttgtcggg
     3721 acggttcagc gaccggaact gccgccagta atttacgcgt ataaggatgc tgcgggtttt
     3781 cgaagaccgc gcgccgtgga ccaatttcaa caatttgccc gagatacatc accgccacac
     3841 gatgactaat ccgctctacc acggccatat cgtgggagat aaacagatac gcaatgccga
     3901 aatcacgctg gagatcgagc aacaagttga taatctgccc gcgaatagaa acatccagcg
     3961 ccgaaacggc ttcgtcggca atgatcactt ttggattcaa tgccaacgcg cgagcaatgc
     4021 agatgcgctg gcgctgaccg ccggaaaact catgcgggta acgccaggca tgttcaggta
     4081 acaggcccac gcgctccagc aaccacgcaa cgcgtgcagc cgcgtcttta cctggcaata
     4141 aaccgtgtac acgcagcggt tcgataatcg aatcaccgat ggtctgacgt gggtccagcg
     4201 aagcgtaagg gtcctgaaaa ataaactgaa tatcccggcg caatgcctga agtttgccgg
     4261 gtgacaaggt atcgattctc tgaccgttaa agataatttc gccgccctgc gattcgacca
     4321 ggcgcagcaa cgcccgcccg gtagtggatt taccgctgcc agactcgccc accagcgata
     4381 gcgtttcgcc aggccagaga tcaaaactga ctttctcaac ggcatgcact tcccgcgtta
     4441 cgcgattcaa caaaccgctg cgcaaaggga aacgggtgac aagattacgc actcgtaaaa
     4501 caggttcgcc atccaccacc gttttctgct cgatgggggg ggcctgtttc gctggatgtt
     4561 caagcgatat caacgggaaa cgtcggggat aatccaaccc tttcatcgca ccaagttgcg
     4621 gaacagcagc taacagcgca cgggtgtaag gatgttgcgg tgcatgaaaa atctgttcga
     4681 cggtacccgt ttccaccgcc tcgccctgat acatcaccag tacccgatcg gcaatctctg
     4741 ccaccacgcc catatcgtga gtgataaaga taacgcccat cgacatctct ttttgcaata
     4801 ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc cagcgcggtg gttggctcat
     4861 cggcaatcag caccgccggg cggcatgaca gcgccatcgc aatcatcact cgctggcgca
     4921 tcccgccaga gagttgatgc ggataacgtg aaagaatggt ttgtgcctca ggaatgcgta
     4981 cctgatccag catccgcttc gcctcgacca tcgcttcttc acgactggcg ttctgatgca
     5041 gacgaattga ttcggcaatc tgttcaccca cagtaaatac cgggttcagc gatgtcatcg
     5101 gctcctgaaa tatcatcgcc atatccgcac cgcgaacatg gcgcatttgt gcagcgctct
     5161 gctcgctaag ttcaatcact tcgcgactgc gccgctgcaa cagcatttta tcgcactgta
     5221 ctaaaccgcc cgcctgttcc aacaggcgca tcaatgccaa cgcagtcact gacttaccgg
     5281 agccggattc gccaacaatt gccagcgtct caccgcgttg cagactaaaa gagagattgc
     5341 ggaccgcagc tattttctgc tggtcctgca taaaggcaat attcagattt tcaaccgcca
     5401 gcacattacc ggcatcaagt tcatcactgt gtggcaacgg tgtccccttt ttcacggtag
     5461 ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc gatacattcc ttcggtgtta
     5521 aacggtagcg cgacattccc ttcatggtcg atagcgatta agccaccgct accgccaagc
     5581 gcagggagtt tttccattac tacccgctcg caggcttccg cgagacttaa tccgccgtaa
     5641 tccattaacg cggcgatgtc atatgccgcc agcgcgcgga tgaagacttc gcccgtgccg
     5701 atacaagaaa ccgccacact ggcgttattg gcgtagcatc cggcacccac taagggacta
     5761 tcgccaactc gtccgggtaa tttattggtc attccgcctg tggacgtggc tgccgccaaa
     5821 ttgccgtcta aatccaacgc cacggccccc acggtgccca ttttctgttt ttcatccagt
     5881 ggcgcaccgc tatggtcgag gacggttgcc ccttccttgc gcgctgccag tagttgttca
     5941 taacgcaaag gcgtggagaa aatctccggc gagacgcgct ccatgccacg agcaaacgca
     6001 aaattttctg ccccttcgcc aatcatcatc acatgcgggc tttgctccat caccagccgg
     6061 gcggcaagaa ccggattacg cagatgacta acgcccgcca ccgcaccggc tttcagggtg
     6121 ttaccatcca tcacacaggc gtccagttca tgggtttcat cacgcgtaaa gacagcgcca
     6181 attccggcgt taaacagtgg acactcttcc agcagacgca ccgcttccgt caccacatcc
     6241 agcgcacttt cgcccgcttc cagcattttc tgcccggttt caacaatggc agacaacgcc
     6301 tcaatgtagc gtaattcctg ttgcagactc atctgcgcgc ggctaattgc acctgcgcca
     6361 ccatgaattg caatgactgc tttgcccata gtattcgtcc attaagggtg gctcgccact
     6421 tttgctataa atatcagaat ttttatgaat tactcattca atttttgaat atagaaagat
     6481 ggatatggca tgtaaaggca ggagccaacg ctacgcccga ggtggcatac agtctgtggc
     6541 gtttttctga cataataggc aaattcgatt ttgcctccgc aggagtgttt tcatggaatt
     6601 taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag atgctttctc gcgtcacccc
     6661 actgaccgcc caggaaacgc tgccactggt acagtgtttt ggtcgtattc tggcgagcga
     6721 tgtcgtttcg ccgcttgatg ttccggggtt tgataactcc gcaatggacg gctacgcggt
     6781 gcgtttagcc gatattgcct ccgggcaacc gctgcccgtt gccggtaaat cctttgccgg
     6841 tcagccgtat catggtgaat ggcctgcggg cacctgcatt cgtattatga ccggtgcgcc
     6901 ggtgccggaa ggctgcgaag cggtggtgat gcaggagcag actgaacaaa cggacaatgg
     6961 cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt cgccgtcgcg gtgaagatat
     7021 ctctgcaggt gcggttgttt tcccggcggg aactcgcctg actaccgcag agctgccagt
     7081 aattgcttca ctggggattg ccgaagttcc ggtgattcgt aaagtgcgtg tagcgctttt
     7141 ttctaccggt gatgaactcc agttgcccgg tcagccgctg ggcgacggcc aaatctacga
     7201 caccaaccgt ctcgccgtac acctgatgtt agaacagctg ggttgcgagg taatcaactt
     7261 agggattatc cgcgacgatc cccatgccct gcgcgccgca tttattgaag ccgacagcca
     7321 ggcggatgta gtgatcagtt ccggcggtgt ttcagtgggt gaagcggatt acaccaaaac
     7381 gattcttgaa gagctggggg agatcgcctt ctggaagctg gcgattaaac caggtaaacc
     7441 gttcgcgttc ggtaaactca gcaatagctg gttctgcggc ctgccgggca acccggtttc
     7501 agcgacgctg accttctatc aactggtaca gcctttgctg gcaaaactaa gcggcaacac
     7561 cgccagcggc ctgcccgcgc gccagcgcgt acgcacagcg tcccgcctga aaaaaacgcc
     7621 aggacgtctt gatttccagc gcggcgtgct gcaacgcaac gccgacggtg aacttgaagt
     7681 gacgaccacc ggacatcagg gttcacatat atttagctcc tttagtctcg gcaactgctt
     7741 tatcgtgctg gaacgcgatc gcggcaatgt ggacgtgggc gaatgggtgg aagtagaacc
     7801 gtttaacgcg ttgttcggag gcctgtaatg gcggaactca gcgatcagga gatgctgcgc
     7861 tacaaccggc aaatcattct gcgcggtttt gattttgacg gtcaggaggc gctaaaagat
     7921 tctcgtgtgc tgatcgtcgg cctcggtgga ctcggctgcg cagcttcgct gtatctggca
     7981 agcgccggtg tcggcaacct gacgctgctc gacttcgaca cggtttcgct ctcgaatctg
     8041 caacgccaga cactgcacag tgatgccacg gtcgggcaac cgaaggtgga atccgcccgt
     8101 gacgccctga cgcggatcaa cccacatatc gcgattacgc cagtcaatgc actgctagat
     8161 gacgcagaac ttgcagcatt gattgctgaa cacgatctgg tgctcgactg tacggataac
     8221 gttgcggtac gtaatcaact gaacgcaggc tgttttgccg cgaaggtgcc gctggtttcc
     8281 ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca cttatcagga cggcgaaccg
     8341 tgctatcgct gccttagccg tttgtttggc gagaacgcat taacctgcgt agaagcaggc
     8401 gtgatggcac cgttgatcgg cgtaattggt tcgttgcagg caatggaagc gatcaaactg
     8461 ctggcaggtt atggcaaacc gaccagcggg aaaatcgtca tgtacgatgc gatgacctgt
     8521 cagtttcgcg aaatgaaact gatgcgcaat ccggggtgtg aggtgtgcgg gcagtaattg
     8581 ccacctccag gcctgataag acgcgacaag cgtcgcatca ggcatttgct ccagacgcca
     8641 gatgcggcgt gaacgcctta tctggcctac ggttcgggca cagatttcca agcgcggcag
     8701 gcatcggtgc actccgccac gtcatcagca atttatgagg atgcagaacg tccggttaaa
     8761 tcgacgttct gccaaacgct ccctgccagt cctgctcaaa cttcgccaca gcggcttcca
     8821 ccgccggata gctaatcatc tgttgtgcca catccagtgg cagagtaatt gattcacatc
     8881 ctgccagtaa gcagtccagc gcctgacgcg gggttttgaa actcgctgcc agcactttcg
     8941 cctgcggcgc atgcattttc aataactggt gtaagtcggt cacagtctga atgccgctac
     9001 cgccctgagc atcaatacga ttaacgtaag gcgcaacata ttccgcacct gccagcgccg
     9061 acagcaaccc ttgtgctgcg ccatataccg cggttcccag cgttggaatc ccttccgctt
     9121 ttaacatctt aatagctgcc agcccctcgg cggtcaccgg aactttcacc acgatatccg
     9181 caataataga acgcaattta cgtgcgtcat tcaccatccc ttcagcagtg gtagccatta
     9241 cctgggcaaa cagacgcccc tgaccgccca tcgcttcatg aagttgcgga agcacaacat
     9301 ccagcggttt tttacccgcg gcgataatgc ttgggttagt ggtcacaccc gccagcggaa
     9361 aaatacgtga cagcgccttc accgcaacaa cgtctgaagt atccagatac agttccataa
     9421 ccatcctcaa aatgtacttt ttgtctgaat gtagactatc acgggaaaac tctctgaaca
     9481 gttgacgcac atcaatctaa ctttcattcg aaagtatttt aatgtttgaa cgaaacagag
     9541 aggcgactat gattttcaat attcagcgct actcgaccca tgatggcccc ggtatccgca
     9601 cggtcgtatt tcttaaaggc tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc
     9661 gcgcccgcac gcaggatctg ctgtatgacg cacgactgtg tctggaaggc tgcgagctgt
     9721 gcgctaaggc cgcgccggaa gtgattgagc gcgcgctgaa tggtttgctt attcatcggg
     9781 aaaagttaac cccggagcat ctgacggcgt taaccgactg ctgtccgaca caggcattaa
     9841 ccgtgtgtgg tgaagtgaaa agcgttgagg agatcatgac gaccgttctg cgcgataaac
     9901 cgttttacga tcgcagcggc ggcggtttaa cgctttcggg tggtgagccc tttatgcagc
     9961 cggaaatggc gatggcgcta ctgcaagcca gccacgaggc aggcattcat actgcggtag
    10021 aaacctgtct gcatgtgccg tggaaatata tcgccccttc tctgccctat atcgatctgt
    10081 ttcttgccga tttaaaacac gttgccgacg cgccgtttaa acagtggacc gacggtaacg
    10141 ccgccagagt gctggataac ctgaaaaaac tcgccgcagc gggcaaaaaa atcattatcc
    10201 gcgtgccgct gattcagggc tttaatgccg acgaaacctc tgtaaaagct attaccgatt
    10261 ttgccgccga cgagctgcac gttggcgaaa ttcatttcct gccctaccac acgctaggca
    10321 tcaacaaata tcacttactt aatctgccct atgacgcccc ggaaaaaccg cttgatgcgc
    10381 cagaactgct cgactttgcc cagcagtatg cctgccagaa agggttaacc gcgaccttac
    10441 gaggataaca accatgacca cactgaaact ggacacgctc agcgaccgca ttaaagcgca
    10501 caaaaatgcg ctggtgcata ttgtgaaacc gccagtctgt accgagcgcg cgcagcacta
    10561 taccgagatg tatcaacaac atctcgataa gccgatcccg gtacgtcgcg cgctggcact
    10621 ggcgcatcac ctggcgaatc gcaccatctg gatcaaacac gatgagttga tcattggcaa
    10681 ccaggcaagc gaagttcgcg ccgcgccgat cttcccggaa tatactgtct cgtggatcga
    10741 aaaagagatt gatgatctgg cagatcgtcc cggtgctggc tttgcggtga gcgaagagaa
    10801 caaacgcgtt ctgcatgaag tgtgcccgtg gtggcgcggt cagaccgtac aggatcgctg
    10861 ctacggcatg tttaccgatg agcaaaaagg tctgctggcg accggaatca ttaaagcgga
    10921 aggcaatatg acctccggcg atgcgcacct ggcggtgaat ttcccgctgc tgctggaaaa
    10981 aggtcttgat ggtctgcgcg agaaagtagc ggaacgtcgc tcgcgcatca atctgacggt
    11041 actggaagat ttgcacggcg aacagttcct gaaagcgatt gatatcgtgc tggtggcagt
    11101 cagcgaacac attgaacgtt ttgcagccct ggcgcgtgaa atggccgcga ccgaaacccg
    11161 cgaaagccgt cgcgatgaac tgctggcgat agcagaaaac tgcgatctta tcgcccacca
    11221 gccgccgcag actttctggc aggcgctgca actgtgttac ttcatccaat tgatattgca
    11281 gatcgaatct aacggtcact cggtatcgtt tggtcgtatg gaccagtatc tctacccgta
    11341 ctatcgccgc gacgttgaac tcaaccagac actggatcgc gaacacgcca tcgaattgct
    11401 gcatagctgc tggctgaaac tgctggaagt gaacaagatc cgctccggct cacactcaaa
    11461 agcctctgcg ggaagtcctc tgtatcagaa cgtcaccatt ggcggacaaa ctctggttga
    11521 tggtcaacca atggacgcgg tgaatccact ctcttacgct atcctcgaat cctgcggtcg
    11581 cctgcgttcc actcagccta acctcagcgt gcgttaccat gcaggaatga gtaatgattt
    11641 cctcgacgcc tgcgtacagg tgatccgttg cggcttcggg atgccggcgt tcaacaacga
    11701 cgaaatcgtg atcccggaat ttattaaact cggtattgaa ccgcaggacg cttacgacta
    11761 cgcagcgatt ggttgtatcg aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg
    11821 catgagcttt atcaacttcg cccgcgtgat gctggcggcg ctggaaggcg ggcgtgatgc
    11881 caccagcggc aaagtgttcc tgccacaaga aaaagcgttg tcggcaggca actttaacaa
    11941 tttcgatgaa gtaatggacg cgtgggatac gcaaatccgt tattacacgc gtaaatcaat
    12001 cgaaatcgaa tatgtcgtcg acaccatgct ggaagagaac gtgcacgata ttctctgctc
    12061 ggcgctggtg gatgactgta ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata
    12121 tgactgggtt tctggcctgc aggttggcat tgccaacctc ggcaacagcc tggcggcagt
    12181 gaagaaactg gtatttgaac agggcgcgat tggtcagcaa cagctggctg ccgcactggc
    12241 ggatgacttc gatggcctga ctcatgagca actgcgtcag cggctgatta acggtgcgcc
    12301 gaagtacggc aacgacgatg ataccgtcga tacgctgctg gctcgcgctt atcagaccta
    12361 tatcgacgaa ctgaaacagt accataaccc gcgctacggt cgtggtccgg ttggcggcaa
    12421 ctattacgcg ggtacctcgt ctatctccgc taacgtaccg tttggcgcgc aaactatggc
    12481 aacaccggac gggcgtaaag cccacacccc gctggcagaa ggcgcaagcc cggcctccgg
    12541 tactgaccat cttggcccta ctgcggtcat tggctcagtg ggtaaactgc ctacggcagc
    12601 gattctcggc ggcgtgttgc tcaaccagaa actgaatccg gcaacgctgg agaacgaatc
    12661 tgacaagcag aaactgatga tcctgctgcg taccttcttt gaagtgcata aaggctggca
    12721 tattcagtac aacatcgttt cccgcgaaac gctgctggag gcgaaaaaac atccggatca
    12781 gtaccgcgat ctggtagtgc gtgtcgcggg ctattccgcc ttcttcaccg cgctctctcc
    12841 agacgcacag gacgatatca tcgcccgtac tgaacatatg ctgtaatctc ctcggcccgg
    12901 cggcgtcgtc gccgggctaa atcattcaca tcatcaattt catccttact ttcattcgaa
    12961 atataatttg tgctctgcgt cacattgtta ttacactgtt acggtttcat tttcgagcca
    13021 ggagcacacc tatgagcgta aaagttatcg tcacagacat ggacggtact tttcttaacg
    13081 acgccaaaac gtacaaccaa ccacgtttta tggcgcaata tcaggaactg aaaaagcgcg
    13141 gcattaagtt cgttgtcgcc agcggtaatc agtattacca gcttatttcg ttctttcctg
    13201 agctaaagga tgagatctct tttgtcgcgg aaaacggcgc actggtttac gaacatggca
    13261 agcaactgtt ccacggcgaa ctgacccgac atgaatcgcg gattgttatt ggcgagttgc
    13321 taaaagataa gcaactcaat tttgtcgcct gcggtctgca aagtgcatat gtcagcgaaa
    13381 atgcccccga agcatttgtc gcactgatgg caaaacacta ccatcgcctg aaagctgtaa
    13441 aagattatca ggagattgac gacgtactgt tcaagttttc gctcaacctg ccagatgaac
    13501 aaatcccgtt agtgatcgac aaactgcaca tagcgctcga tggcattatg aaacccgtca
    13561 ccagtggttt tggctttatc gacctgatta ttcccggtct acataaagca aacggtattt
    13621 cgcggttact gaaacgctgg gatctgtcac cgcaaaatgt ggtagcgatt ggcgacagcg
    13681 gtaacgatgc ggagatgctg aaaatggcgc gttattcctt tgcgatgggc aatgctgcgg
    13741 aaaacattaa acaaatcgcc cgttacgcta ccgatgataa taatcatgaa ggcgcgctga
    13801 atgtgattca ggcggtgctg gataacacat ccccttttaa cagctgacct ctctaccgga
    13861 gccgtgcgct ccggttttcc cttcttttta catcctgtac atcacatcaa atcattaaac
    13921 ttgcattaac ttatttataa cttatattgt cgcatgcaaa tcagaatggt ttcgaatgaa
    13981 agttaatgag aggtggttat ggcttctact tttaccagcg acacattgcc tgccgatcac
    14041 aaagcagcta tccgtcagat gaagcacgcg ctgcgggcgc agcttggcga cgtccagcag
    14101 atctttaatc agctaagcga tgacattgcc acgcgagtgg ctgaaatcaa cgcactcaaa
    14161 gcacagggcg atgccgtctg gccggtgctg tcttatgccg atatcaaagc aggtcatgtt
    14221 actgcagagc agcgcgaaca gattaaacgt cgcggttgtg cggtgataaa aggccatttc
    14281 ccccgcgaac aagcgctagg ctgggatcag tcgatgctgg actatctgga ccgcaaccgc
    14341 tttgacgagg tctacaaagg ccccggcgat aatttcttcg ggacgctcag cgcttcacgt
    14401 cccgagattt accccatcta ctggtcgcag gcgcaaatgc aggcccgcca gagtgaagaa
    14461 atggcgaatg cgcagtcgtt tctcaatcgt ctgtggacat ttgaaagtga tggaaagcaa
    14521 tggtttaacc cggatgtgag cgtcatctac cctgaccgta tccgccgccg tccgcccgga
    14581 acgacctcca aaggtcttgg agcgcatacc gactccgggg cgctggaacg ctggctgctt
    14641 ccagcgtatc agcgcgtttt cgccaacgtc tttaatggca atctggcgca atatgatccc
    14701 tggcatgcgg cacatcgtac ggaagttgaa gagtacacgg tggacaacac caccaaatgt
    14761 tccgtgtttc ggacattcca aggctggaca gcgctctcta atatgctgcc tggtcagggg
    14821 ctgctgcacg ttgtgcccat tcctgaagcc atggcgtacg tactgttacg tccgctgctt
    14881 gatgatgtgc cggaggatga actgtgcggc gtagcgcccg gaagagtgtt gccggtatca
    14941 gagcaatggc atccactgtt aattgaggcg ttaaccagca ttccaaaact cgaggccgga
    15001 gactccgtct ggtggcactg cgacgtcatc cattccgttg cccccgttga aaatcaacag
    15061 ggttggggca acgtgatgta cattcctgcg gcaccaatgt gcgagaaaaa tcttgcctac
    15121 gcgcacaagg tgaaggccgc actggaaaaa ggcgcatcgc cgggcgactt cccgcgcgag
    15181 gactatgaaa caaactggga aggacgcttt acgcttgccg acctcaacat tcacggtaag
    15241 cgagcgttgg gaatcgacag ctgagtgtcg tacagccctt cccgttccac ggcggtgttc
    15301 ccttttcctg ggcgaacccg ccgcactgac tcccgcaccg ccaatcgtcc gtgcagcagc
    15361 agcgagccca ccggagcatc cgggcgaatc agccgctgtt gcaacatttg caccgcttca
    15421 ttgcctagct catcgcgtgg cacatgcaca gccgtcaggg gaacatcctc gattgccgca
    15481 aggttaaagc cgtcgatact catcaccgag acatcctgtg gtacgcgcag accacgacga
    15541 tgtagcgcat ttaccgttcc cgccgccata aaatccccgc cgaccagaaa cgccgtcggc
    15601 aacgtcttgc cattggcggc atccagccac gctccgacct tttcttccgc ttctttagcg
    15661 ctaaaactgc tgatattcag caaatgacgg tcatggtgaa aggccagatt atgctgacgc
    15721 catgattctt taatccctgc aaaccgctgc tccatagtgt agcggcgcag gcacatcaca
    15781 ttcatcacct cccggtgacc catttcaaac agatagcggg cggcgaacgc gccaattaac
    15841 cgatgatccg gcgcaatcgc cgggaggcgc attcgttcat cacggcagtt gatcagcaca
    15901 caaggcttag cgagatcggc ggccagatca tggatgtgcg gatcgtcaat ccccagcagg
    15961 atcgctgcct gcgtatctgc ttcattcatc cgcgagagaa agagcgcggg atcgctgtca
    16021 aactcctcca gcgcgcagta acgcagccgc acttcatgcg gcgataacgc tttgctgatg
    16081 ctctggatca cacggtaata aaagatatca gatcgttggt caaaagcgcg ttgcggcgcg
    16141 aaaataatca ggttgttcag caacatacga cctgctgcca tgccatccat cacgcccaac
    16201 tcacgcgcgc aggccagcac ctgttcgcga gcgcgtgcac tggtattcgc tttgccagcc
    16261 agcacccgcg aaaccgtact cggcgatagc ccggtacgtg cggcgatatc ggcgattttc
    16321 agcttcttgt ccattttgtg atctgcctct aattcggaaa tgacagaatt ttcatgagca
    16381 aaaaagccca ttctgtctgg cattaaatcc atttcagtaa tgtatatcag tcactcatgg
    16441 aaaaacgtgc acaaaacccg ctttctttcg cttttagggt ctcttactgt gaattggtag
    16501 aacaacttcc atacaaaaat aatggaaaaa cgaacaataa agactttatt tatcaaaaag
    16561 ttaaaagtgg tgtaacaact ccattctgac gttgccgcac aaaccctata ccattctgtg
    16621 gagtgcatat gagtcagggt atcaataacg atatgactgc cagtaaaccg cgccatatcg
    16681 tcaaaaatct gcgttggtgg atactggtcc tttttttgct cggtgttact gtcaactaca
    16741 tcacgcgtaa ctcgctgggt attctcgccc ccgaactgaa aagcagccta gggattacca
    16801 ccgaacaata ttcctggatt gtaggtgctt tccagctcgc ctatacccta ttccaaccgt
    16861 tgtgcggatg gctgattgac gtcattggcc tcaaactggg ctttatgatc tgcgcgggac
    16921 tgtgggcact ggcctgtctg atgcatgcgg gggcgggaaa ctggggacat ctggcgctgt
    16981 tgcgcttctt tatgggcggt gcggaagccg cggcaacccc agcaaacgcc aaaaccatcg
    17041 gtgaatggtt tccaaaatca gaacgcccga tcgccgcagg ctgggcaggc gtcggttttt
    17101 ccatcggtgc catgctggcc ccaccgatta tctattttgc ccacgcttca ttcggctggc
    17161 agggtgcatt tatgttcact ggcatcctgg cgatgctgtg ggtggtgctg tggtgggcgt
    17221 tctatcacag cccggataag catcctaacc tcagcaaagc cgagctggac tttattcgcc
    17281 aggataacga agcgcccccg gtcaagttac cctttctgac cgcactgaaa accgtgggca
    17341 aaaataaacg cttttacggt atcgctatcc cggcctttat ggcagaaccg gcgtgggcgg
    17401 tgatgagttt ctgggtgcct ctatacctgt ccaaaaccta tggcatggac ctgaagcaga
    17461 tcgcattatt cgcctggttg ccgttcctcg ccgctgatct tggcagtatt gccagcggct
    17521 atctgaccaa actttacacc cgcctgtttg ggctgagtcg tgtgaactcc gttgtcgcat
    17581 catcggtcag tggcgcattc ttgatggttt cgctggcggt gatggccttt accaataacc
    17641 cgtatatcgc catcgtcctt atctccatcg gcggcttcgg tcaccagatc atctcctgca
    17701 tgctgagcgc actggtggtg gaatccttcg acaaaggtca gatggccact gttaacggca
    17761 tgcgcggttc tgccgcgtgg atcgcaagtt tcctgttctc cctgattatc ggggttaccg
    17821 ctgacaaaat cggctttaac cccctgttta tcgccatggg cttctttgac ctgattggcg
    17881 ctttcttcct ggtcaccttt attgctgaac gtcgcgcaaa acgcgcctga gagtggatgt
    17941 aaacatgaaa acgctgaaaa actggaccct gcacaaacag tctgcccacc acgttgaact
    18001 gctggtcgat ggccagcata tcctgtgcct gtacgtgctg gaagaaaatc tgttccgcgt
    18061 gctgttaaaa cgcaaaggcg agctggcgct agatcgcacc tggagcattg ctccggcgca
    18121 ggatgtgcca tgggaagggc gcagccgcga agatctcagc ggctttagcc tgcccgcgtg
    18181 gacagtgatc cagcacgacg acaccgtggt tatcgccacc gagtcactgc gtgcaaccgt
    18241 tcaccagccg ctgtggctgg agtggcacta tcgtaacaac gaaggcgaat ggctgccgtt
    18301 ggtgaatgac cgcccgacca gcgcttatct gctgaatgcc cacggcgatg gcgtggcaca
    18361 ctatcagagc cgtcgtaaag atgaacgctt ttatggcctc ggagataaag ccggcgatct
    18421 gcaacgcaac ggtaaacgtt acgagatgcg taacctcgac gcgatgggct acaacgccgt
    18481 tagtactgac ccgctgtaca aacacattcc attcaccatc acccgccgcg atgacattag
    18541 cttcggcctg ttttatgaca acctcagcag ctgctggctg gatctgggca acgagatcga
    18601 caactaccac accgcctatc gccgctggca ggcggaagcg ggtgatatcg actactacct
    18661 gtttaccggc gaacgcgtac ttgatatcac caaagccttc gtacgcctga ccggaaaaac
    18721 cctgttcgga ccgaaatgga gcctgggcta cagcggttca actatgcact acaccgatgc
    18781 gccggacgca caaaatcagt tgatgaactt tatccgcctg tgtgaagaac atgcaattcc
    18841 ctgcgattcg ttccagctct cttccggtta tacctcaatt aacggcaaac gctatgtgtt
    18901 caactggaac tacgacaaag tgccgcagcc gaaagtgatg agccaggcat ttcacaatgc
    18961 tggtttgcac ctggccgcca atatcaagcc gtgcctgttg caggatcacc cacgctataa
    19021 cgaagtggca gaaagaggac tgttcatccg tgattcagaa acagatgaac cagaacgttc
    19081 cagcttctgg gatgacgaag gttctaacct cgagtttact aatccgcaga ccatccagtg
    19141 gtggcaggaa ggcgtgacca cacagcttct ggagatgggt atcgactcca cctggaacga
    19201 caacaacgaa ttcgaagtat gggacggtga agcacgctgt cacggctttg gtcagacgat
    19261 cgcgatcaaa catattcgcc cagtgatgcc actgctgatg atgcgagcct cgatggaagc
    19321 ccagcagcgt ttcgctccgg aaaaacgtcc gtacttgatc tcccgctccg gctgcgcggg
    19381 aatgcagcgc tacgtgcaga cctggagtgg cgataaccgc actagctggg acactcttcg
    19441 ctacaacacc cgcatgggtc tggggatgag cctctccggg ctgtataacc tcggtcacga
    19501 cgtcggcggt ttctccggtg ataagccgga cccggaactg tttgtacgct gggtacaaaa
    19561 cggcgtgatg catccgcgct ttacgattca ctcatggaac gatgaccata ccgtaaacga
    19621 accgtggatg tatccgggcg tcactccggc gatccgcagc gctattgagt tgcgctatcg
    19681 cctgctgccc tacttttata ccctgatgtg gcaggcatac gccgatgatg aaccaatgct
    19741 acgtccgact ttccttgacc acgagcacga cgttcagact tttgaagagt gtgatgactt
    19801 cctgcttggt cgcgatattt tggttgccag cgtcgtagag caaggcgagc gccagcgccg
    19861 tgtatggctg ccagataacg aaactggctg gtacgatttc tacaacggcg aatggttcag
    19921 tggcggtcag tggatcaccc tcgacgcacc gctggagaaa ctgccgctga tggttcgtgc
    19981 cggggctggt ctaccgctca gcaaacgcat cacctatgtg agcgcagaac aagacgatac
    20041 ccgcgaactg aaactgttcc cgctgaaagg tgtgggcaca acgtccggcc tgctgtttga
    20101 agacgatggc gaaagctggg gctaccaaac tggtaatgcg ctttgggtgg aatgggaaat
    20161 ggtttgcgat ggggcgacca tcaacctgaa agtcaacgcg cgcggggatt atcgtcctgc
    20221 atggagtgca ctgaaagtgt cattaccatt ggaagaaaaa cgcacgctgc tggtcaatgg
    20281 tgttgaaggg agtgaatgga tgcgttaaat cattaccggg gccgggaggc gactgcacct
    20341 tacccggtcc agccatcgaa tcctttacct cagtatttca cgctgcagga aggcagcaaa
    20401 cctgtaaacc ccgggagctt aaataagtaa gtgaccgggg tgaacaggtg aagccaatgc
    20461 atctgccgca tgaaagacgg cggggaatta agcgtcgatc cctttactac gcaggtaatc
    20521 ttcgtaatta ccgctaaagt cgatcgcgcg ttccggggtg atttccagaa tgcgggtcgc
    20581 cagggagctt acgaactcac ggtcgtgtga aacaaagatc agcgtgccct gatacagttc
    20641 cagcgccatg ttcagtgact cgatggattc catatccagg tggttggtcg gttcgtccat
    20701 gatcagaatg ttcggcttct gcatcattaa cttaccaaac agcatccgcc ctttttcccc
    20761 accggaaagc actttagctg gctttttgat gtcgtcctgg ctgaacagca aacgaccgag
    20821 aatgctgcgt accgcctgct cgtcatcgcc ttcctgcttc cactgactca tccattcgaa
    20881 cacggtcaga tcattttcaa actcatattc gtggtcctgg gcgtagtaac caatgcgcgc
    20941 gttttcagac cattttacgg tgccgctgtc cggttgcaga tcgcccacca gcgttttcag
    21001 cagcgttgat ttaccgacgc cgttggtacc cagtaccgca agtttttcac cgacctccag
    21061 cagcaggttg agatttttaa acagtggacc gttatcaaac cctttggtca gaccttccac
    21121 ttccagcgcg ttacggaaca gtttcttatc ctgttcaaaa cggatgaacg ggttctgacg
    21181 gctggaggct ttcacctctt ccagtttgat tttatcaatc tggcgcgcgc gcgaagttgc
    21241 ctggcgagat ttcgaggcgt tggcgctaaa gcggctaacg aaagattgca actcagcaat
    21301 ctgcgctttc ttcttggcgt tatcggccag cagacgttca cgcgcctggg tcgccgccgt
    21361 catgtactca tcgtagttac ccggataaac gcgcagctcg ccgtaatcca gatccgccat
    21421 gtgggtacag accatgttaa ggaagtgacg gtcgtgcgag atgatgatca tggtgctgtc
    21481 acgctcgttc agcacctgtt ccagccagcg aatggtgtcg atgtcgaggt tgttggtcgg
    21541 ttcgtcgagc aggagaatct ccggatcagc aaacagcgcc tgcgccagaa gcacacgcag
    21601 tttccagcca ggagcaactt cactcatcgg gccgtagtgc tgttccactg gaattcccac
    21661 gccaagcaac agttcaccgg cgcgagcttc cgcagagtaa ccgtccattt cgccgtattt
    21721 aacttccaga tcggccactt tatagccgtc ttcctcactc atttccggca aagcatagat
    21781 gcggtcacgc tcctgcttca cttcccacaa ctctttatgc cccatgatca ccgtatccag
    21841 cacagtgaac tcttcaaagg caaactgatc ctgacgcagc ttaccaatgc gctcgttggg
    21901 atcgagggaa acgttaccca aagtcggctc taagtcgccg ccgaggatct tcataaaggt
    21961 ggatttacca ctaccgttcg cgccaatcag gccgtaacgg ttgccgccgc cgaatttgac
    22021 ggaaatgttt tcaaacaacg gcttactgcc gaactgcatg gtgacgttac tggaaactaa
    22081 cacaggcgta tcctgaaaag agatatgaca aaccgcgcat tatgccataa gtctgaaaga
    22141 agatcctgct cctcgcttaa ggtctacact caagccaaag gcaaaaaatg tgatttcgta
    22201 cacatctgat ttcactgtga gctggaatga acttataatg cgcttccaat actctaatat
    22261 tctcaaccca atggcctgcc aggcacaaaa tctcgcttaa catgaatatg aaattgaaaa
    22321 cattattcgc agcggccttc gctgttgtcg gcttttgcag taccgcctct gcggtaactt
    22381 atcctctgcc aaccgacggg agtcgcctgg ttggtcagaa tcaggtgatc accattcctg
    22441 aaggtaacac tcagccgctg gagtattttg ccgcggagta ccagatgggg ctttccaata
    22501 tgatggaagc gaacccgggt gtggatacct tcctgccgaa aggcggtact gtactgaaca
    22561 ttccgcagca gctgatcctg ccggataccg ttcatgaagg catcgtcatt aacagtgcag
    22621 agatgcgtct gtattactat ccgaaaggga ccaacaccgt tatcgtgctg ccgatcggca
    22681 ttggtcagtt aggcaaagat acgcctatca actggaccac caaagttgag cgtaagaaag
    22741 caggcccgac ctggacgccg accgccaaaa tgcacgcaga gtaccgcgct gcgggcgaac
    22801 cgcttccggc tgtcgttccg gcaggtccgg ataacccgat ggggctgtat gcactctaca
    22861 tcggtcgcct gtacgctatc catggcacca acgccaactt cggtatcggc ctgcgtgtaa
    22921 gtcatggttg tgtgcgtctg cgtaacgaag acatcaaatt cctgttcgag aaagtaccgg
    22981 tcggtacccg cgtacagttt attgatgagc cggtaaaagc gaccaccgag ccagacggca
    23041 gccgttatat tgaagtccat aacccgctgt ctaccaccga agcccagttt gaaggtcagg
    23101 aaattgtgcc aattaccctg acgaagagcg tgcaaacagt gaccggtcag ccagatgttg
    23161 accaggttgt tcttgatgaa gcgattaaaa accgctccgg gatgccggtt cgtctgaatt
    23221 aatcttcaaa acttaaagca aaaggcggac tcataatccg ccttttttat ttgccagacc
    23281 ctagttggcc gggagtataa ctaacaaaac atatcccacc aacgccgccc acaacagcat
    23341 cggtattgaa tagagatgga aacgccacca gatgcggcga tcgttcgcca tacgtagcgc
    23401 aatcaaattt gccagcgatc cgggtaataa cccaaagcca cctacgttta ccgcccatac
    23461 cagtaataaa gacggcggca catagttcag caacaatatg gtactcggca cgttactgat
    23521 cacctgcgat aaaccgattg ccgttaacca taacccgggt tcagatagat gactcacgtt
    23581 acccaacacg ccttgcaacg ctggaagctg ggtcagtaaa tggacgtcga taaacatcgc
    23641 cataaacacc agcagcagcg tccagtccac actgagcacc acgcgacgtg ccagcagcgc
    23701 aaagcctgcc gccacaatca ccagtcccca cagctcttgt ttgaactcca gcgccgtcag
    23761 aaagacgata tacagcccca gacaactcca caccagccgc ggtttccact ccggtgtttg
    23821 cacccccgta tggtattgca gcgcctttcc agggaaacaa caccagcaca ggagcaggag
    23881 cgtcagcatc attgcgccag ccagcggtgc catttgggca ataaatccgg caaacgaaag
    23941 accagaacgt ccccagataa gaatattttg cgggttgcca attggcgtca gtagcgaacc
    24001 agcgttgact gccagcgcct caaaaataat cagccgatta accgggatct cacacagtct
    24061 ttttagcgtg atagtcagcg gaacaacaat aaacagcgcg acatcgttgg tcagaaaggt
    24121 agaaagcagc gccgccgcca gcaccataaa catcgccagc cgacgctccg tagcaaagcg
    24181 gcgcaccatt ttgcgcccca gcacatcaaa ataaccgctt aactccacac ctttggtcag
    24241 cagcatcagg ccgcttaagg tgatgatggt gtgccagtcg atagcagcag gccaggattt
    24301 cggtgcaaag ggcacgaaaa agcttaatcc gataccaaca agaattaata actgaaaaaa
    24361 acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg tttctgggta
    24421 aacaaacgaa aagcgtcgag cgtctcttca ctaacatggt gctccatgcc ttccgcgtcg
    24481 cgacgggcga tttccggact gacgcccaac accaacaaga aattttcgac tatctgatgt
    24541 cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac gccacgccag
    24601 gggatcattt caatcagccc catggttgcc agccgcttaa gcattttagc caccgtcggt
    24661 tgcgaaactc ccagacgagc agccatgtcc acctgacgag cttcccccac ttccctgatc
    24721 aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc ctctctgacc
    24781 tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt ttttgctgtt
    24841 ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata catagcgttc
    24901 tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta acgttttagc
    24961 aatagctaca taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc cctcaaggat
    25021 cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaagtgta tgcgcgtgtt
    25081 tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg atatggtcaa
    25141 caacaaaccg cagcaagata aaccttccga taaatagcgg cgtcgcggta cgccgcttca
    25201 ctcctgcttt tatgcaggca taacgcgttt tggtctgaaa aaccccactt tttgtcggat
    25261 ttgcaatccc cttcgcaaaa gatttgttcg tcagtagttg acctgaacgg cggctcgctc
    25321 tatcttcttg cagccctacg tatattgcgg ctcgcggatg cggacccctt tcccctcttc
    25381 acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt ttgttacgat
    25441 agtggcatat gaatttaacc ctcaaagaat cgcttgttac ccgtagccgg gtatttagcc
    25501 cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta ggttacccct
    25561 tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg cgaacattgc
    25621 ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt tatttccctt
    25681 ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac tccaccaata
    25741 tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg gtcggcttat
    25801 ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa aaagcgcgct
    25861 ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttt gttgctcccg
    25921 tgcaaaacct ggcctggggt ggcgtattta aactgaaaga tactggctat ccggtatttc
    25981 gttttgctaa ggatgtcatc gtcaataata acgaggtgat tgaagagcaa gaacggatgg
    26041 caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg aagtatcaga
    26101 cctatgtggt ggtgatcggc gaaagcgcgc gtcgcgatgc cctcggtgcc tttggcggtc
    26161 actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct gactacattg
    26221 ccgccagtgg ctccacgcag aaatcacttg gcttaacgct caatcgcgta gtcgatggca
    26281 aaccacagtt tcaggataac tttgtcaccc tggcaaatcg cgcgggcttc cagacctggt
    26341 ggttttccaa ccagggccaa atcggcgaat acgatactgc tatcgccagc atcgccaaac
    26401 gtgcagatga agtgtacttc ctgaaagaag gtaatttcga agcagataaa aacacgaaag
    26461 acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg caaccgcagc
    26521 tgattgttct gcatctgatg ggctcgcatc cgcaggcctg cgacaggaca caaggcaaat
    26581 acgaaacctt tgtgcaatcg aaagaaacgt cgtgctatct ctataccatg acgcaaacgg
    26641 acgatttact gcgcaagctg tacgatcagt tacgcaacag cggcagcagc ttctctctgg
    26701 tttacttttc tgaccacggt ctggccttta aagagcgcgg caaagacgtg caataccttg
    26761 cccatgatga taaatatcag caaaatttcc aggtgccttt tatggtcatt tccagcgacg
    26821 ataaagcgca tcgggtgatt aaagcccgcc gctcagccaa tgacttctta ggctttttct
    26881 cgcagtggac gggaattaaa gcgaaggaaa taaatatcaa atacccgttt atatctgaga
    26941 agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtggat tacaaccatc
    27001 tcggaaccga tattttcgac ccgaaacctt aaaacaaaaa atccgccccg agaggcggat
    27061 tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca ggtgcctacg
    27121 tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt ttccatcggg
    27181 ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg tttgtaggtc
    27241 gggtattcag tggtctggaa tttaccataa cccaccccca ctacaccgta gatgcttgcc
    27301 cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt tttgttgtag
    27361 tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat cacacccagc
    27421 gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt gttcatttgg
    27481 ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc tacggaagta
    27541 cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt tttcataacc
    27601 acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt aatgggaaac
    27661 tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt ccatacaccg
    27721 ctatccatct aaatttt
//
LOCUS       DAZOYL010000018        27688 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZOYL010000018 DAZOYL010000000
VERSION     DAZOYL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27688
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.018"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..317
                     /gene="tnpA"
                     /locus_tag="ACHUOY_000569"
                     /pseudo
     CDS             1..317
                     /gene="tnpA"
                     /locus_tag="ACHUOY_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            377..451
                     /locus_tag="ACHUOY_000570"
                     /pseudo
     CDS             377..451
                     /locus_tag="ACHUOY_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(448..1771)
                     /locus_tag="ACHUOY_000571"
                     /pseudo
     CDS             complement(448..1771)
                     /locus_tag="ACHUOY_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1771..3387)
                     /locus_tag="ACHUOY_000572"
     CDS             complement(1771..3387)
                     /locus_tag="ACHUOY_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0681453.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3683..4399
                     /locus_tag="ACHUOY_000573"
     CDS             3683..4399
                     /locus_tag="ACHUOY_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0681454.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4396..6057)
                     /locus_tag="ACHUOY_000574"
     CDS             complement(4396..6057)
                     /locus_tag="ACHUOY_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR0681455.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6253..6681)
                     /gene="ibpB"
                     /locus_tag="ACHUOY_000575"
     CDS             complement(6253..6681)
                     /gene="ibpB"
                     /locus_tag="ACHUOY_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR0681456.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6793..7206)
                     /gene="ibpA"
                     /locus_tag="ACHUOY_000576"
     CDS             complement(6793..7206)
                     /gene="ibpA"
                     /locus_tag="ACHUOY_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR0681457.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7200..7272)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7512..7844
                     /locus_tag="ACHUOY_000577"
     CDS             7512..7844
                     /locus_tag="ACHUOY_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0681458.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7846..9060)
                     /locus_tag="ACHUOY_000578"
     CDS             complement(7846..9060)
                     /locus_tag="ACHUOY_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR0681459.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9161..10225
                     /gene="cbrA"
                     /locus_tag="ACHUOY_000579"
     CDS             9161..10225
                     /gene="cbrA"
                     /locus_tag="ACHUOY_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR0681460.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10222..11559)
                     /locus_tag="ACHUOY_000580"
     CDS             complement(10222..11559)
                     /locus_tag="ACHUOY_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0681461.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11634..12782)
                     /gene="dgoD"
                     /locus_tag="ACHUOY_000581"
                     /pseudo
     CDS             complement(11634..12782)
                     /gene="dgoD"
                     /locus_tag="ACHUOY_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12779..13396)
                     /gene="dgoA"
                     /locus_tag="ACHUOY_000582"
     CDS             complement(12779..13396)
                     /gene="dgoA"
                     /locus_tag="ACHUOY_000582"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR0681462.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13380..14258)
                     /gene="dgoK"
                     /locus_tag="ACHUOY_000583"
     CDS             complement(13380..14258)
                     /gene="dgoK"
                     /locus_tag="ACHUOY_000583"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR0681463.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14255..14944)
                     /gene="dgoR"
                     /locus_tag="ACHUOY_000584"
     CDS             complement(14255..14944)
                     /gene="dgoR"
                     /locus_tag="ACHUOY_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR0681464.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14913..15545
                     /locus_tag="ACHUOY_000585"
                     /pseudo
     CDS             14913..15545
                     /locus_tag="ACHUOY_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341111.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15591..16403)
                     /gene="yidA"
                     /locus_tag="ACHUOY_000586"
     CDS             complement(15591..16403)
                     /gene="yidA"
                     /locus_tag="ACHUOY_000586"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR0681465.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16518..16916)
                     /locus_tag="ACHUOY_000587"
     CDS             complement(16518..16916)
                     /locus_tag="ACHUOY_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR0681466.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17156..19570)
                     /gene="gyrB"
                     /locus_tag="ACHUOY_000588"
     CDS             complement(17156..19570)
                     /gene="gyrB"
                     /locus_tag="ACHUOY_000588"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR0681467.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19599..20672)
                     /gene="recF"
                     /locus_tag="ACHUOY_000589"
     CDS             complement(19599..20672)
                     /gene="recF"
                     /locus_tag="ACHUOY_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR0681468.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20672..21772)
                     /gene="dnaN"
                     /locus_tag="ACHUOY_000590"
     CDS             complement(20672..21772)
                     /gene="dnaN"
                     /locus_tag="ACHUOY_000590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR0681469.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21777..23180)
                     /gene="dnaA"
                     /locus_tag="ACHUOY_000591"
     CDS             complement(21777..23180)
                     /gene="dnaA"
                     /locus_tag="ACHUOY_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR0681470.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23477..23557
                     /locus_tag="ACHUOY_000592"
     CDS             23477..23557
                     /locus_tag="ACHUOY_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681471.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            23787..23927
                     /gene="rpmH"
                     /locus_tag="ACHUOY_000593"
     CDS             23787..23927
                     /gene="rpmH"
                     /locus_tag="ACHUOY_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR0681472.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23944..24303
                     /gene="rnpA"
                     /locus_tag="ACHUOY_000594"
     CDS             23944..24303
                     /gene="rnpA"
                     /locus_tag="ACHUOY_000594"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR0681473.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24267..24524
                     /gene="yidD"
                     /locus_tag="ACHUOY_000595"
     CDS             24267..24524
                     /gene="yidD"
                     /locus_tag="ACHUOY_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR0681474.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24527..26173
                     /gene="yidC"
                     /locus_tag="ACHUOY_000596"
     CDS             24527..26173
                     /gene="yidC"
                     /locus_tag="ACHUOY_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR0681475.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26279..27643
                     /gene="mnmE"
                     /locus_tag="ACHUOY_000597"
     CDS             26279..27643
                     /gene="mnmE"
                     /locus_tag="ACHUOY_000597"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR0681476.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6654 a         7500 c         7084 g         6450 t
ORIGIN      
        1 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
       61 aactggttta aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      121 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg ttactgcgac
      181 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtcctggct
      241 attttgccaa tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      301 agcaaacacc tggttaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggacggggtt
      361 ttacggcgca ccggataaat ttggttatgc gccgtcagcc gtgctaaaga atacggacca
      421 gcacccaacg gatgccagtc cacacaatta acgtaactct ggccagtacg ctttgttggc
      481 ctccagcaac tcatccagaa tagctttggc gaccgaggcg ctcggtaccg ttttcgataa
      541 cgtaatcgcc tgccacaggt gctgatatga acgctgttcc caggcgtcca cgaccagttt
      601 ttccaccgcc acttgctgac tcattaaccc tttctgaaac tgcgggatat cgccgaccac
      661 cagtggttct ggtccgttgt gcccgaccag acacgggatc tcgaccatcg cttcatcatc
      721 aaagttatgg attgccccgt tgttaggcac aatcagcaac atccgctcct gagtgttgaa
      781 ggcaattgcc gccgccagat cgacgatgta tgacgcatgt tcgtcaatct ccagcttggc
      841 cgccgctgaa tttcctgccg ctgtaatggc gcggcaagca tcgaaaacct gtttctcgcg
      901 atgttccatt cacctcattc gcgcgggtat gttcagggtt ggaatgctga acaacataat
      961 ccgggaagag ataatatttc agataggtgt tcggcaatgt atccgggtcc agtgcctgga
     1021 catcccgcgc ttttgcatag gtgtcgttcc agctcgcttc agtatgtgta tcttgctgcg
     1081 gtggaacata accgtgcttc gagacatatt gccgcagctg gggcatcagg tcgttgccct
     1141 gcaaatcttc aattgatgtc caccagccaa agtggttcag gccgtagtag cgcacgcgca
     1201 tctgtttgcg atcttgcagc ccaacaattt gcgccatccg gctttcaata ccgattggca
     1261 tgtcacagat gttgaggatt ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa
     1321 tggctgccgg gttggagtag ttgagcatcc aggcatttgg tgaatatttt tccatataat
     1381 ccaccagttc caggacgccg ccaatggaac gcatgccgta cgctattccg ccgggtccgc
     1441 aagtttcctg accaacaaca ccgtggcgca gtgggatttt ttcatccagt tcgcgcatcg
     1501 ggtacttgcc gacgcggatg tgcgccatga caaaatcaac gtcgctgaac gccacttcag
     1561 gatcggtggt gtaactaaac gcaatgtccg gcgctttctc tttaaggatg actttacagg
     1621 cttcggcaat cacttcctgc cgcgcgccat cgttatcata aaatttcagt gcacgaagcg
     1681 ggaaacgctc ctgattcgcc aggagcatca acacgatgcc tggcgtaaag gtgcttccac
     1741 cgcctgcgac aaccactgag aatttggtca ttatactgcc tccgtaatgg caacattttc
     1801 tgctgattga tgagaattaa ttaagctatc gagctgttca cgcagctggg atacatgcag
     1861 accaatgatc acctgaatgg catcgccact acggaagacg ccgtgcgctc ccagcttttt
     1921 aaagacttcg tcatccagcg tttgtgacat gtcatgcagt gcaatgcgta gacgcgtcgc
     1981 acaattgtta atgctggaga tattgccgac cccgcccagg gcttgcagga taccggcagc
     2041 ctgatccagc tctttttttg gctctgccgc ggtggtttgg cctcgcgagg ctttgtattc
     2101 ggcttttgag tagagtttca cttccgcatc ttcacgtccc ggcgtgcaca tgttgaactg
     2161 cagaatcagg gtgcggaaaa ccacgaagta cagcagggta aagcacaacc caatggcgat
     2221 ttgggtcagc atcatatccg cgtggttgct gaacatcggg atccagtttt gcggtaaaac
     2281 ctggtcaatc agacctccgc ccatgttgcc caccacacca aagagataca tcacggtcga
     2341 cattgaggcc gcaagcacag cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt
     2401 aaattccagc ggttcggtaa ttcccaccag catggcggtt aaggttgcgg gaatcagcaa
     2461 gcccgccact tttacccgat tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc
     2521 cacggcacca aagatttttg agttaccgtg cagggcaaaa ccgccttccg ggaacaacga
     2581 tttcagcggc tcggcgctca aactgaactc ttgcagatgc tgcgcccagt acatctgaat
     2641 gccgccttca acagctgccg gacccaagat aaacggtccg tagatgaagt ggtgtaaacc
     2701 ggttgggatc agaatacgtt cgaggaaggt gtaaacccag accccaagcg cacccgccga
     2761 acgcaggaac gcttgcagag attcaatccc cgtttgtact tttggccagc cgagcaatgt
     2821 cagccaggca caggggatca tcaccaggaa ggcgataatc accacataag acgtcccctg
     2881 gaaaatgccg agaaaaaccg gcagtttttt atcgaacaga cggttatgca gcgccgtcac
     2941 aatgccggaa atgataattg cgccgataat gctggtatcg agggttttaa tcccggccat
     3001 cattgtcaga ccgctacctg ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc
     3061 ccaggtcatt cccatcgcgt tgatgaaata gttccaggtc aggaaactca ccatcaccgc
     3121 cagacaagca cgtccctgcg cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat
     3181 cagcggcata ttacggaata ccgtccaacc gccctcttca ataatgtgta cgatttgcgc
     3241 gaataaactg ttcggatcgg tcagtgattc cccgacaaac atcgggtttt gcagcaagat
     3301 ggcaagaccc accacaatcc cggcgaaggg aaacagcagc actggcgtaa acatcgcgcc
     3361 gccaaagcgt tgaatttgac tgagcatttc cacatcctct tttctcaatt ctgtaagtag
     3421 gaagcctgat tggttatgcg gcaggcctgg agcaaggata ggaagatgcg atcggcagaa
     3481 catgacgacc caatgggatt cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct
     3541 acgttttcgc gaaaaagtag acatctgtga gcacaggtac acagcatcga acacatcttt
     3601 aaaaaaaaga tgttttttca atcgattaag cagaacttgt gtgcgcatta cccgggcttg
     3661 caggcaaaaa agagatctag agatgatcta caaaagcatt gcggagcggt taagaattcg
     3721 acttaactcc gcagatttca cgctaaacag ccttcttccg ggtgaaaaaa agctggcgga
     3781 agagtttgcg gtatcgcgga tgaccatccg taaagccatt gacctgctgg tagcgtgggg
     3841 gctggtggtc cgccgccacg gtagtggcac ttacctggtg cgcaaagatg tactgcatca
     3901 aaccgccagt ctgaccgggc tggtggaggt gttaaaacgg cagggaaaaa ctgtcaccag
     3961 ccaggtgctg atttttgaaa tcatgcctgc gcctccggcc attgccagcc agttacggat
     4021 tcaaatcaac gagcagatct acttctcccg tcgcgttcgt tttgtggaag ggaaaccgct
     4081 gatgctggaa gacagctata tgccggtaaa actgttccgt aatctttcgc tgcaacatct
     4141 ggaagggtcg aagtttgaat atattgaaca agagtgcggg attttgattg gcggtaatta
     4201 tgaaagcctg acgccggtgc tcgccgatag actgctggcg cggcaaatga aggtagcgga
     4261 acacacgcca ctgctgcgga tcacctcgtt gtcatatagc gagagcgggg agtttttgaa
     4321 ttattcagtg atgttcagaa atgccagcga ataccaggtg gagtaccatt tacggcgact
     4381 ccacccggaa aagagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat
     4441 aatgcgcagg aacatcacta acggatagac agtggcgtaa gagagcgccg ccgcaccgct
     4501 agttggatga agattattag caaacgccag cgccggagga tcggtcatgg aacctgccag
     4561 catcccgcac atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt
     4621 aatcagcgga acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatt
     4681 gaccagggta ttcacaaaat ccccaccaga tttcagacca acgaccgaga ggaacagcac
     4741 gatccccagc tcccgcagcg cgaggttggc acttggcggc ataaaccagt acagcttgcc
     4801 gatactgccg atacgcccga ggatcaacgc cataatcagc agaccgcccg ccagccccag
     4861 tttcaacgcg gccgggaatc ccggcacaaa gacgggaata gaacctaaca atacgccaag
     4921 cccgatgcca ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc
     4981 cagcacattg gcaacggcat caattgcgga cggacgcccc accaggttga ggatatcgcc
     5041 gaactgcagg ctgatatcgc cactggcgac cagttcgacc ccggcacggt tcaggcgcga
     5101 gataacaacg tcatagcgtt ctttaaagtg caggtcgcga atacgttttc cgagcacgtt
     5161 ttcattggtg accaccacac gctcgacgcg caaatcagtg tctttcgtgg acagcgaagt
     5221 atcgacctcc tgaccaatca ccagttgcgc attatgtaaa tccgctggct gacccaccag
     5281 atgcagcaaa tcgcccagtt ggataatggt atctggcgaa ggaactttta gggtttcttc
     5341 gcgtttcaga cgcgagcaga taattttgtc gccgttgaga atcggtacat ctttaatggc
     5401 taaatcatgc aggttagggt tctcaacgcg aatattgata gtcttgatca gcgcgccgcc
     5461 attggtgcgt gaagactcgt gctgctgagc ttctgtctcg acattgacgc ggaaaataac
     5521 ccgcaacatc cacatggtga acaaaatccc gcaaatgccg aatggatacg ccatcgcgta
     5581 actcatcccc atctgatcga ccatttccat tggtgtaccc aggtcgcgca aaatctgctg
     5641 ccctgccccc agcgctggcg tattggtaac cgcaccggag aaaatcccca gcactaccgg
     5701 cagtggaata tcaaacagtt tatgcaggat ggcggtaacc agaccaccga tgatgacgat
     5761 cagaacagca aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg
     5821 ccctacctga atcccgatgg tataaacgaa caggatcagg ccaaattcct gaataacatg
     5881 cagcatatcg ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc
     5941 accaaacagc acgccgccaa tacctaatcc tatgccgcga aatttgacgt tgccgataaa
     6001 caaaccgacg actgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt
     6061 ccatttctct tgagataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga
     6121 tttgccttat agcgcacatt attacgggaa agaacgtgcc gaaatatctt aaacagtcga
     6181 aatttaacgc attgttgaag aagaaagaca aaaaaagccc caccagaatg gcggggcaaa
     6241 gagaatagct agttagctat ttaacgcggg acgttcgctg atagcgatac gctgcgctgc
     6301 gatgggttca ggctcattac gaattaaatc aatatgcagt aaaccgttta cgaaagttgc
     6361 gccagagact tccatatttt cagccagcgt aaagctcagg ctaaatggct gattcataag
     6421 cccttgatgc agccattttt tctcttcttt tggctgctcc ggcgtgcctt ttacgctcag
     6481 gcgcgtacct tccagttgaa tctctaaatc ttcctgacgg aaacctgcca gcgcaagggt
     6541 aatgcggtag tggttatcgt cgcttttctc aatgttgtac ggcgggaagc tctgactttc
     6601 accggcgttt tgcagtgcgt tggccagttt gtcaaaaccg atccattgac gcatcagtgg
     6661 ggataaatcg aagttacgca tagtcatttc tccttctaag aagcgagtaa gtacctgcaa
     6721 atccgaagat tcgcatatgc tccctgacgg cgagcatgga gatgtcaggc cgcgccaggc
     6781 ggccttaagg aattagttga tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg
     6841 ttcgagatcg atatacagca aaccatttac caggttagcg ccacgaacat gaatgttctc
     6901 agctaactgg aatttgcgtt caaagttgcg ttcagcgatg ccctggtaca gataggtgcg
     6961 ctctttttgt tcgtcggcgt gagcaccttt caccaccagc agattatcct gggcggtaat
     7021 ttccagttcg ctctcagcaa aaccagccac agcgatagca atgcggtaat ggttttcgtc
     7081 taccagttca acgttatacg gagggtagcc gccattactc tggctctggt tgttttctaa
     7141 gtggttaaac aaacggtcaa atccaatagc agaacggtaa agcggggata aatcaaagtt
     7201 acgcataatc aatagctcct gaaatcagcg agaatgtaag accttccaca atggacaggt
     7261 caggtagcca gaacacccat caggcgcgtc ctcatcgact acgatgtaaa aatgggtctg
     7321 gaaatgaact tttcaagcct tatcagcgga cttttttttg cagtttatgg tctattgcat
     7381 agactgaggg ggcagcacga aacgttaaag tgtgatagcc gccacagagc gacgtaatga
     7441 tgatgttatt ttcagcaagg gatcgctata actcatcagg ccaacaccca cgatcaacag
     7501 atgaatttat tatgataaga aatgtgttgt tagcgttcat gatatgcagc ggaatggcat
     7561 tactcggagg atgctccagc gtgatgtccc ataccggagg gaaagaagga acgtatcccg
     7621 gcacccgcgc cagcgccacc atgattggcg atgatgaaac caactggggc actaagtcgc
     7681 tggcgattct cgatatgccg tttaccgctg tgatggacac gcttttatta ccgtgggatg
     7741 tgttccgcaa agacagctcg gtgcgctcac gcgttgaaaa aagcgaagcc aacgcgcaag
     7801 ccaccaatgc ggttattcca ccggccagaa tgcctgataa ctgatttagc gatcagtttc
     7861 ggtgatccac agctggccac cttccatcca cgccagccat tgaccatccg gcgagaagac
     7921 cacggcgtcc gcagaaggtg gattggcgtg gttttcggtt aaatactcaa cctcgccgct
     7981 ttgcgcatgg gcacaagcaa ttcgattatc cagcacaaag cccaaccatt ctcctgacgg
     8041 atgccagtta aatgcagact gaatatccgt tttgttatgg gttaactggc gcggctcgcc
     8101 gccctgtggc gagataagcc acagttgcac aatgccgtta tcatcacgca ttaaaaacgc
     8161 gatttgcgta ccctgcggat tacagcgcac ccagtggcgc gggacgttga ctaaccccgg
     8221 ataagcccga tggtgggtaa aggttaaacg tcgctgcacg acgccacgcg gtggcgcggg
     8281 cagggtggtt tccgttccac ttaacggcgc atcacctgcc gctttccagc cagcttcatc
     8341 ttgcggcaac tcaacgataa acagctccgg cactttctca ccctttggcg aaagtgtgtc
     8401 gccaataaac gccagcgcgt gatttcctac ccatccttct tcataagcac gattgatttc
     8461 atcactgcca ggctgcggcg tgggcgtggt tttactcacc agcacacacc agtggctgcc
     8521 gctgtattca cgcggatgct gcttttgtac gttgaccgga ccaaacggcg cagcaacacc
     8581 gacgtttcgc aaatccagcg ccggatcgag ttcatgcatt acatggtcgt tataggtaaa
     8641 gctcaccctt tcaccgttcg ggctaaagac atgcacatgg ctgccgccgc gcagcgctcc
     8701 tggcgtatac ggcgcggtaa tatccattgc atcgagattg ctcaccttgc cgccttcagc
     8761 aatgactccg cgccgatgat ggaagtcgta atgccatgtt tcatcaggat tttccgggcc
     8821 gtgaataaag acatatttct ctgacttcgg atgaacggtc accacgccga cgtgtgcgcc
     8881 ctgtgacgca cagtagataa cctcgacctc accggtatgg atattcacac gctcaatggt
     8941 ctcgccggta aacgacgccc cagaaggacg cacgtcaaat accagccact ggctgtcggg
     9001 cgtccaggta ttggtattgg tgagcaggtg attacgggga gcaaaggtta tctgtttcat
     9061 ggcgcggtcc tgatacaaaa cgggtccagc catcatactt cacaaggact tcactttcag
     9121 acaatagtct tttgccatcg tgatgaatgt gagtgaaaag atggaacatt tcgacgtggc
     9181 gattattggc ctcggcccgg cagggtcggc gttggcacga aagttagccg gcaaaatgca
     9241 ggtgatcgcg ctggataaaa agcaccagca tggtactgaa ggtttcagca aaccttgtgg
     9301 cggtctgctg gcaccggacg cgcagcgatc ttttattcgc gatgggctga cgcttcctgt
     9361 cgatgtgatc gctaatccgc agattttcag cgtcaaaacc gtcgacgtcg ccgcatcgct
     9421 cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg acttgtggat
     9481 gaaatcgctg atccccgcca gcgttgaggt ttatcacgac agcctgtgtc gaaaaatctg
     9541 gcgtgaggat gataaatggc atgtcatttt tcgtgcagac ggctgggagc agcatattac
     9601 cgcccgctat ctggtcggtg ccgatggtgc caactcgatg gtgcggcgac atctctaccc
     9661 ggatcatcaa atccgtaaat atgtcgcaat ccagcagtgg ttcgcagaga aacatccggt
     9721 gccgttctac tcctgcatct ttgataatgc gataactgac tgttattcat ggagtatcag
     9781 caaagacggt tattttatct ttggcggtgc ctatccaatg aaagacggtc agacgcgttt
     9841 cacgactctg aaagagaaaa tgagcgcctt tcagttccag tttggcaaag cggtgaaaag
     9901 cgaaaaatgc acggtgctgt ttccctcacg ctggcaggat tttgtctgcg gtaaggacaa
     9961 cgcctttctg attggtgaag cggcgggatt tatcagcgcc agctcgctgg aggggattag
    10021 ctatgcgctg gatagcgcag acattctgcg ttcggtgtta ctgaagcagc cagagaagct
    10081 caataccgct tactggcgcg ccacccgcaa actgcgttta aaactcttcg gcaagatagt
    10141 aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca gtggtgtggc
    10201 acatattcca cagttgaagg attagccaac gcgcttcaca tcgcccacca gcaggatata
    10261 agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggtg cgaaaccgta
    10321 accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac caccggcgaa
    10381 gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag aaaccagcga
    10441 ccaggtaatt gaagcaaaac cgttaccgaa gaatgccagc gccatcaggc acataatcat
    10501 catcggatcg ttagtgtagt tagcgcccat aatgcaggtg gagatcagca agccgcagat
    10561 aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca gatccgctac
    10621 ccagccagag agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg tcataaagcc
    10681 tgctttcagc gccgtgattc ctttttcctg ggttaaatag ttcgggaacc aggttaagaa
    10741 aaaccacagt gtagaagcca ccgcaaattg cccaagatag acgccgatca gtttacgatg
    10801 gaacaccagt ttccagtctt tggctgttaa cggctgacgt gcctctttct tcactggcgc
    10861 atcaccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt tgctgatgcc
    10921 tttggtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga taatgccgat
    10981 accaccagtc acaatgaaca cccagtgcca gctcaacatc tcctgaatcc agatcagcag
    11041 cggagtcaaa aacgccagac cgacaaactg accagacgta taaaaaccaa cggcggaggc
    11101 gcgttcatgt tccgggaacc agctggtcac catccggtta ttggtcggga aagcgggcgc
    11161 ttcgaaaata ccggttatcg cacgcagacc aattaatgac attaatcccg tggcaaagcc
    11221 ctggaataaa gtcgccactg accagccaaa tatcgcaata aaataagtca cgcgagaacc
    11281 tacgcgatct aaaaaccaac cgccggggat ctggcatagc gtataaagcc aggcgaaggc
    11341 cgaaaataca tagcccattt ccgctttcgt aatgccgaac tcttcctgaa tatgggcgga
    11401 agccacggcc aggttagcgc ggtcaacata acaaatgacc accgtaataa agatcatcac
    11461 cagcgtcaga taacgccgac gccccggctt tgctgcatta acgggaatat ccatagcgag
    11521 ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt tacagagggt
    11581 tatatttttg tattgctgtt ttagtgcccg atgaggggct tacgtggcag gaattaccac
    11641 tctgctacgc tgttatcttc atgacgccag agtggattac gccagtccgg ggcattttta
    11701 ctgaactcaa taactttagc ttcgtcgatt tccacgccta agcccggttt cgttagcggt
    11761 ttaaagaagc cgccaaccat gctgaagtct tctttgtttt tcacaaagtc gagtaactcc
    11821 gcgcctttgt tgtaatgaat tcccatactt tgttcctgaa gtaccgcgtt ataggaaaca
    11881 aagtcgatat gcagacaagc cgccagtgca atcggtccga gcggacagtg cggcgcaagg
    11941 gtcacgtcat aggcttctgc cattccggca attttgtagc attcggtaat accgcctgca
    12001 tgggagagat ccggttgtag aatcgaaata ccgcctgcct ccagcacgcg tttaaaatcg
    12061 aaacgtgaga acatgcgctc acccgccgcc agtggaatat gcgtttgtgc cgccaatttc
    12121 gggtagtatt cggcttgttc cgccagcacc ggctcttcaa taaacagcgg gcgatacggc
    12181 tccagttctt taatcagcac tttcgccatt ggcgcgctga cgcgaccgtg gaaatcaaga
    12241 ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt gactgccgca
    12301 tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag tttgaaggta
    12361 tcgaagccga tttcgcgcag ggttttaata ccgtcgatca catccgccgg acgatcgccg
    12421 ccgacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag ttgccagacc
    12481 gacgcattca gcactttacc tttgatatcc cataacgcct ggtcgatccc ggcaatggcg
    12541 ctcatcagga ttggaccgcc acgataaaat ccggcgcgat acatcacttg ccataagtca
    12601 ttgatgcgct atggatcctg accaatcaaa tagtcaccca gctcgtgaac tgccgcttcc
    12661 accgtgcggg cgcggccttc aatcactggc tcgccccagc cgacgacgcc ttcatcggtt
    12721 tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt ggtgattttc
    12781 attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg gtgcgttcta
    12841 cggactgccc ggcgcgatag agatcgctgc ctaagcccgc cccagcacaa cctgcgtcta
    12901 tccactgcgc caggttttct ggcgtcacgc cgccaacggc aaagactgcg atgtccgatg
    12961 gcaataccgc ttttaacgct ttgatgtatt gcggaccaaa agccgatgac ggaaatattt
    13021 tcagcgcctg cgcgcccgct tcgagcgcgg taaaggcttc ggtcgccgtc gcgcagccgg
    13081 ggcagacggt catgccgtag cccaccgcac ggcggatcac ttcactatgg atattgggcg
    13141 taacgatgag ctgacagccc atcctggcga gcgcatcgac ctgttcaggt ttcagtaccg
    13201 tacctgcgcc aatcaacgcc ttgtcgccat acgcatcaac gatggcggga atgctttgct
    13261 cccattgtgg ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca atcaccgcgc
    13321 caacatgcgc cagcgcctcg tcgggcttaa tgccgcgcaa aatggcgatc agcggaagtt
    13381 tagtttgcca ctgcatgagc gatgctcctt ataccagcct gaaatgccgt gtcgcccgcc
    13441 accgccgtca cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc ggtcaacgat
    13501 gttccggcga caagggtgat ggcgtgttga tgggccacat agtcgcgcat actggcgacc
    13561 tctgcgccaa tcaacaaacc agagagaaat tcactgacct gttcacgggg aagtgttccc
    13621 agcacatgcg aggcacgaac ttcaaaaagc tgcggcaata tggcgggcgt attaagacca
    13681 cgctcaaggc cagccgtgaa ggcatcggca gagttttcct gcggcggcaa acctgcacca
    13741 atcaatgagt gatttaacag taaatgatgt aattcaccgg tcatcacggt gcgaaaatcg
    13801 ttgatttgct ggctatcggc ctgcacccat ttgcaatgcg taccgggcat gacataaaga
    13861 gaggaaggag ccagagctcg cgcgccgatc aattgagttt cttcgccgcg catcacattg
    13921 tggttatcat catgagagac gcataatccg ggaataatcc agatattgtc gccaacagac
    13981 gttaattgtt cgccaataga cgaaaaatgg gcaggaacag ataaatacgg agcaactttc
    14041 cagccgacat tgctgccgac cattcctgcc attaccactg gcgtattctc ttcacgccag
    14101 tcggtcgtga cttctgctaa caccgcagcc ggagattttc cgttcaggcg cgtgacgcct
    14161 gcttctgatt gcctgctctc caggcagtgg tcgccctgat aaagccaggc gcgcagattg
    14221 gtcgatcccc agtcaattgc gatgtagcga gctgtcatgt gatttccttt aaccttcgtg
    14281 tcgagctggc gatcatggta agcgccgcct gctctgccgc atcgccgtcc tgatgccgta
    14341 tcgcatcgaa cagcgcctta tgttcctgga gcgtttgcgg catgttggcc tcatcgccca
    14401 tccaggttcg ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta agttgctgta
    14461 acaccgggtt atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa
    14521 acgcttcgcg gtcctgattg ttggcaatca tctcgttcag cgccgattca atctgcgcca
    14581 gatcgctgga ggtcgcgcgc tctgctgccc aacgggcaat cgccggttcc accagatttc
    14641 gcacttcgct catggcactg ataagccgtg ggtcgtagtc gttttccagc acccattgca
    14701 gtacgtcagt gtcgaggtaa ttccactggt tacgcggtgc cacaaacgcg ccgcgataac
    14761 gtttcatttc aatcagccgt ttcgccatca acgaacggaa cacctcacgg atgatgttgc
    14821 gcgaggttgc aaactcctca cagagttccg cctcagccgg aagcggcgag cctggtacgt
    14881 atttgccgtg aacgatctgt ttacccagcg taatgacgat gcgatcggtt ttattgagag
    14941 tcatggagag tccttgtgct ctgtatgttc ttctctactt taccccgatc gatgcataat
    15001 caggaaagta tcaatcgcag tcagacacca tcacggggct atcgcaggca aaggatagta
    15061 atggaacaaa aggctatgtt tttgtagggg aatcgctgga ttaccttatc acagatggtg
    15121 ccgatgacat cgttaagatg ctcaaggatc cagctcttaa ccggcacaat attcaggttg
    15181 ctgatgacgc aagatttgtt ttaaatgcgg ggaaaaagaa atttaccggc acaatatcac
    15241 tctattacaa ctggagcaca gaagaagaaa aagcacgagt gacacaatat cgttttgctt
    15301 gtggtgttca gcactgtacc cggacagtag aaaacctgga aggtactatc catgagaaaa
    15361 ataaaaacat ggattactca aacgtaatgg ctttctacca tccatttaaa gtgcgatttt
    15421 atgaatacca ttcacccaga ggcattccgg atggtgtttc cgcagcatta ctgccagtga
    15481 ctgttacgct ggacatcatt actgcaccgc tgcaatttct ggttgtatat gcagtaaacc
    15541 aataatcagt aagcgggcaa acgcgtttat gctgtttgcc cgcccacaga ttaattcagc
    15601 acatacttct caatagcaaa cgccacgcca tcttcaaggt tagatttggt gacaaagttc
    15661 gccacttctt tcaccgaagg aatagcgtta tccatcgcca caccgacgcc tgcatattca
    15721 atcattgcga tgtcgttttc ctgatcgcca atcgccatga tttcttccgg tttaatacct
    15781 aacacgtcgg ccagtgattt cacacccgta cctttgttaa cgcgtttatc gaggatttcg
    15841 aggaagtacg gcgcactttt cagcacggta tatttctctt tcacttcctg cggaatacgc
    15901 gcgatagcct ggtcgaggat ggcgggttca tcaatcatca tcactttcag gaactgggta
    15961 ttggggtcca ttttctccgc ttcgcagaac accagcggaa tagtggcaac gaaagattca
    16021 tgcactgtgt agtagctgat atcgcggttg gcggtgtaca gcgtggtgcg gtccagggcg
    16081 tggaaatgag aaccgacttc gcgagagagt ttttccagga aacgatagtc gtcatagctg
    16141 agagcagttt gcgccacggt gctaccatca gctgccttct gtactagcgc gccgttataa
    16201 gtaatgcagt agtcgcccgg ctgttccata tgcagctctt tcaggtagtt gtgcacacct
    16261 gcatacgggc gacccgtcgt tagcacgaca ttcacgccac gggcgcgagc tgcggcaatc
    16321 gcatttttaa cggcgggtga aatggtgtga tcgggcagca gaagggtgcc atccatatcg
    16381 atagcaatga gtttgatagc catgagttcc ccaggtagat tggttcctga cccatgctaa
    16441 cgcgattccg ctcaaaaatc agtacaacac ccgagggaaa agggggatgc aacgcgggtt
    16501 gcatgctccc catttgctta gcggaagagt ttccctttca gcagttccat acctgcggaa
    16561 agcagatcgt tattggcttg tggtgacact tcaccttgcg gtgagagcgc atcaataatc
    16621 ttcggcaatt gctctgccag taaactggaa gctgtactgg tatccacgcc aagtttttgc
    16681 ccgagatcgg acaccgcatt tgtgccgagc gccgattcca gttgctcgcc actaaccgat
    16741 tgattgccct gttgattact cagccaggtt gagagaatgg cccctaagcc gccactttgc
    16801 agtttttcca gcagcacctg aatgccgccc tgctcctcaa cccaacttaa aatagcctga
    16861 tatttccccg catcgccttt cagaaaggca ccgacaactt catcaaaaag ccccatgata
    16921 atcacctgta aagcgttacg tgttgaccca aaaagtatag atttgcggga tgataattgc
    16981 ggattgcaga aataaaaagg gcggagatga tctccgccct tttcttatag cttcttgccg
    17041 gatgcggcgt gaacgcctta tccggcctac aaaatcatga aaattcaata cattgcaaga
    17101 ttttcgtagg cctgataagc gtagcgcatc aggcacgctc gcatggttag cgccattaaa
    17161 tatcgatatt cgccgctttc agggcgttct cttcaataaa cgcacggcgc ggttcaacgg
    17221 cgtcgcccat cagcgtggtg aacaactggt cggcagcaat cgcatcttta acggtaacgc
    17281 gcagcatacg acggctttcc gggtccatgg tggtttccca cagctgttcc gggttcatct
    17341 cgcccagacc tttataacgc tggatggaga ggccgcgacg ggactctttc accagccagt
    17401 ccagcgcctg ctcgaagctg gctaccggct gacgacgctc gccacgttcg ataaacgcat
    17461 cttcttccag caagccacgc agtttctcac ccagcgtgca gatacgacga tattcgccac
    17521 cggtgataaa ctcgtgatcc agcggatagt cagtatccac accgtgggta cgcacgcgaa
    17581 caatcggttc aaacaggttt tgttcggcat tggtgtggac atcaaacttc cactggctgc
    17641 cgtgctgttc tttgtcgttc agttcgctga ccagcgcgtt cacccagcgg gtaacggtct
    17701 gctcatcaga gaggtcggct tccgtcagcg tcggctgata gataagctct ttcagcattg
    17761 ctttcggata acgacgctcc atgcggttga tcattttctg cgtcgcgttg tactcagaca
    17821 ccaatttctc taacgcttcg ccagccaatg ccggtgcgct ggcgttggtg tgcagcgttg
    17881 cgccatccag cgcgatagag atctggtact gatccatcgc ttcgtcgtct ttaatgtact
    17941 gttcttgctt gcctttcttc actttgtaca gcggcggctg agcgatgtag acgtgaccgc
    18001 gttcaacgat ttccggcatc tgacgataga aaaaggtcaa cagcagcgta cgaatgtgcg
    18061 agccgtcgac gtccgcatcg gtcatgatga tgatgctgtg ataacgcagt ttgtccgggt
    18121 tgtactcgtc acgaccgata ccacagccaa gcgcggtgat aagcgtcgcc acttcctgag
    18181 aagagagcat cttatcgaag cgcgctttct caacgttgag gattttaccc ttcagcggca
    18241 gaatcgcctg gttcttgcgg ttacgcccct gcttcgcaga gccgcccgcg gagtcccctt
    18301 ccaccaggta cagttcggaa agcgccggat cgcgttcctg gcagtctgcc agtttgcccg
    18361 gcaggcccgc taagtcgagc gcacctttac ggcgggtcat ttcacgcgcg cgacgcgccg
    18421 cttcacgggc acgggcagca tcgataattt tgccaaccac gattttcgcg tcggttgggt
    18481 tttccagcag gtattctgcc agcagttcgt tcatctgctg ttcaaccgct gatttcacct
    18541 cagaagaaac cagtttgtct ttggtctggg aggagaattt cgggtccggc actttcacgg
    18601 aaacgaccgc aatcaggcct tcacgcgcat cgtcaccggt ggcgctaact ttggcttttt
    18661 tgctgtagcc ttctttgtcc atgtaggcgt tcagggtacg ggtcatcgcc gcacggaagc
    18721 ctgccaggtg agtaccgccg tcacgctgcg gaatgttgtt ggtaaagcag tagatgtttt
    18781 cctggaagcc atcgttccac tgcaacgcca cttcgacgcc aataccgtct ttttcagtgg
    18841 agaagtagaa gatattcggg tggatcggcg ttttgttctt gttcagatat tcaacgaacg
    18901 ccttgatgcc gccttcatag tggaagtggt cttctttgcc gtcgcgcttg tcgcgcagac
    18961 gaatggaaac gccggagttg aggaacgaca actcacgcag acgtttcgcc agaatttcat
    19021 attcgaactc ggtcacattg gtgaaggttt cgaggctggg ccagaaacgc accatggtgc
    19081 cggttttttc agtctcgccg gtaaccgcca acggtgcctg cggtacaccg tgttcgtaga
    19141 tctgacggtg aattttaccc tcgcgctgga taaccagctc cagtttttgc gacagggcgt
    19201 ttactaccga aacaccaacg ccgtgcagac cgccggacac tttataggag ttatcgtcaa
    19261 atttaccgcc tgcgtgcaga acggtcatga tcacttccgc cgccgatacg ccctcttccg
    19321 ggtgaatacc ggtcggaatg ccgcgcccgt catcctgtac agagacagag ttatcggcgt
    19381 gaatggtgac gataatttct ttacagtgac ccgcgagcgc ttcgtcgata gcgttatcta
    19441 ccacctcgaa taccatgtgg tgcagaccgg tgccgtcatc cgtgtcgccg atatacatac
    19501 ccgggcgctt acgcaccgca tccagccctt tcaggacttt gatactggag gagtcataag
    19561 aattcgacat caacgtttct cgctcattta tacttgggtt aatccgttat tttacccttt
    19621 tccacggtaa acatcttcga attttcgtcc gacatgtcta taacgtgttc agcactgatc
    19681 gcgctgacaa agacctgtga ttgcgtcgct tttaagcggc tggcaagcag cccgcgacgc
    19741 tcatcatcaa gctcagaggc aaaatcatct atcaggtaga gacaccgccg cccgctttca
    19801 cgggtgagga actctccttg cgccagacgt aaggcgcaca tcaacagctt aagctgccca
    19861 cgcgataagg tatcttccac cggcgcaccg tcggcgcgaa tgcgtaagtc cgctttatgc
    19921 gggccatgcg cggtgtaggt tagctggcga tcgcgttcaa aattacgttc cagcacctca
    19981 gcatattctg tctctttctc ccagccgcgc tggaaagaga aagtcagaga aaactcaggg
    20041 agaaattgct tacaagtatc ggccatatca gccgcgatac cggcgctata ctccgcgcgc
    20101 caggtgctga tttgctccgc cagcgggatc agctctttat cccacgggcg tagctgttcg
    20161 taacgtgtca cctggcgcag cgccgcattg cgctgcttga gcaatcgctt gagattgctc
    20221 caggcggtga aaaatccggg ttcgttgtga aagcatcccc agtcgaggaa tgctcttctg
    20281 tatttggggc cgccgttgag taaagtaaac ccttctggcg ttatcaactg cattggcatc
    20341 aggtgcgcca gttccgcgac cttatgcccg tctgtaccgt cgatgcggac tttgctgtcg
    20401 ccctgtttgt ctttggttaa gccaatcgct gtctcgcgct cttcgccctg taatcgcccg
    20461 tggagaacaa acgcctcctg ctcatggcga atgacgcgac caatctgcaa actgcgaaac
    20521 gcccgaccat ggccgagcgt atagatggct tccagcacgc tggttttgcc actgccgttg
    20581 gcacctacca gaaagttaaa gccgggagat aaagcgagat ccgcggtttc aatgttgcgg
    20641 aaatcgcgga tcaacaagcg ggtgagggac attacagtct cattggcatg acaacatagg
    20701 cagccgactg tgatgcggca tcttcaatct gcacgctgga aaccgaatcg gtcagcatca
    20761 tgcggacgtt ttcacatttc agcgcgttca gaacatccag cacgtagctg acgttgaagc
    20821 cgatttccat ctccgcaccg ctataggtaa cgtcgaggat ctcttccgct tcttcctgtt
    20881 ccgggttgtt ggcggtgatt ttcagctggt tttcgctgac ataaagacgt acgccgcgga
    20941 atttctcgtt agagagaatc gccgcgcgag caaacgcctg cttgagcaga tcgcagccag
    21001 cttccagatg tttgtccggg ttcttcggca gaacgcggcg ataatccggg aagcgaccat
    21061 ccaccagttt ggaggtgaag ataaagtcgc caacgtgggc gcgaatgttg ttgctgccaa
    21121 tctgtacgcg cagcggattg tcgccgccgt cgagcatacg catcagttca atcacgcctt
    21181 tacgcggcac gatcaccgaa tggcttggca aagattgacc aattggcatt gaacagactg
    21241 ccagacggtg gccgtcggtc gccacggtgc gcagttcttc gccttcggtt tcaaacagca
    21301 taccgtttaa gtaatagcga acgtcctgat gcgccataga aaactgggtc gcttcaatca
    21361 gacgcttcat cgtcgcctgc ggcagggtaa attcgacttc gctctgccag tcatcgaggt
    21421 tcgggaaatc cgccgctggc agggtagaca gcgaaaaacg gctacgcccg gagcgcacca
    21481 gcatccgttc accttccagc tgtacggcaa tttccgcgcc ttcaggcaga ccacggcaga
    21541 tatcaaagaa tttgcgcgcc ggaacggtcg tcgctcctgg ctcgtgtggc tgaaccagcg
    21601 caacgcgtgc caccatttcc atctcgagat cagtaccggt cagcgacaac gtaccgtcag
    21661 caacctgtaa cagcagatta ccgagaatcg gtagcgtagg acgaccacct aacggcccgc
    21721 tcacctgttg tagcggtttt aataaatgct cacgttctac ggtaaatttc ataggtttac
    21781 gatgacaatg ttctgattaa atttgaaaaa tcttctttga tatcgtggct ctcttcacgc
    21841 aactgctcga tcttacggca ggcatgaagc accgtcgtgt ggtcacgacc accaaacgca
    21901 tcgccaatct ccggcagact gtggttagtc agctctttcg ccagcgccat cgccatctgg
    21961 cgcggacgcg ccaccgagcg ggatcgacgc ttggaaagga gatccgcgac tttgatcttg
    22021 tagtactccg ccaccgtctt ctgaatattg tcgatggtga ccagtttttc ctgcaatgcc
    22081 agcaagtcgc gcagtgcctc acgcacgaag tcgatggtga tcgcccgtcc ggtaaagttg
    22141 gcgttagcaa taacgcggtt cagcgcccct tccagctcac gtacgttaga tcgtagacgc
    22201 ttggcgataa agaacgccac ttcgcctggc aaacgaatgt cattttcgtc ggcctttttc
    22261 atcaggatcg ccacgcgggt ttccagctct ggcggttcga tcgccacagt cagtccccaa
    22321 ccgaagcggg atttcaaacg atcctcaacg ccgttgatct ctttcggata gcgatccgag
    22381 gtgagaatga tctgttgatt accttccagc agggcgttga aggtgtggaa aaactcttcc
    22441 tgagatcgtt ctttattagc aaaaaactga atatcgtcga tcagcagtgc atctacggaa
    22501 cggtagtagc gtttaaactc ttcgatcgcg ttgttttgca gggctttaac catgtcctga
    22561 acaaagcgct cggagtgcat ataaaccact ttggcattcg gcttgcgcgc cataatgccg
    22621 ttacccaccg catgcagcag gtgagtttta cccagacccg tgccgccata aaggaacaat
    22681 gggttatagg caccgccagg gttatccgcc acctggcgag ccgccgcgcg cgccagttgg
    22741 ttagatttac cttcaacgaa gttatcaaac gtgtgtttga cgtttacgtt agaacgatag
    22801 gtcggttctg ccggggccgg gacgttatcc caacctgagc gcgtagaagg cgcagcacgt
    22861 tgcggctgcg tttgcgccac ctgtgcaggg gccgcgacgt tgctcgtcac tgccgcttgt
    22921 ggcgtttgcg tcaccggttt ggtgccgact tcaaaacgca gctgtggggc atccgctccg
    22981 cagaaactgg ttagcagtcc attgatatta ttaaggtact tgtcccgtac ccaatcgagg
    23041 acaaaacggt ttggcgcgta cagggccagc gtgttatcgc tcagttccgc ctgcaatggg
    23101 cgtatccaca tactgaattc tgtggctggt aactcatcct gcaatcgggc aagacactgc
    23161 tgccaaagcg aaagtgacac ggcggactcc actcgaacaa aagtcgataa tgactaaggc
    23221 tgaaacattc atgattgttg acgtacgtcg aaaagaccct gcttgagggt gacgcacgaa
    23281 ccgctgtctg cggttatatg cccgatcaag atcctgcaaa acgatcggga ccgcggatca
    23341 tagcctaaac tgcgcaagag atcttctgtt tctcacagat tttcccgatt tatccacagg
    23401 actttccaga cctcgctaag tgtaaacgat cctgccgcga ggcgggcacg atttacgccg
    23461 catattggaa aatttaatga ccatagacaa aaattggctt aatcgatcta ataaagatct
    23521 aggacgatcc ttgcgcttta cccatcagcc cgtataatcc tccacccggc gcgccatgct
    23581 ggttttcact ggtgtgaggt cgtacatttt ccctgcgaaa aggtgcggaa aagcgcggta
    23641 aataaggaaa gagaattgac tccggagtgt acaattatta caatccggcc tctttaatca
    23701 cccatggctt cggtgtccat cgtttcattt ttcggcggat atccaataaa gccattgaat
    23761 ttattcaagt ttaggtagaa atcgccatga aacgcacttt tcaaccgtct gtactgaagc
    23821 gcaaccgttc tcacggcttc cgtgctcgta tggctactaa aaatggtcgt caggttctgg
    23881 cacgtcgtcg tgctaaaggc cgcgctcgtc tgaccgtttc taagtaataa agctaacccc
    23941 tgagtggtta agctcgcatt tcccagggag ttacgcttgt taactcccag tcaattcaca
    24001 ttcgtcttcc agcagccaca acgggctggc acgccgcaaa ttaccattct cggccgcctg
    24061 aattcgctgg ggcatccccg tatcggtctt acagtcgcca agaaaaacgt tcgacgcgcc
    24121 catgaacgca atcggattaa acgtctgacg cgtgaaagct tccgtctgcg ccaacatgaa
    24181 ctcccggcta tggatttcgt ggtggtggcg aaaaaagggg ttgccgacct cgataaccgt
    24241 gctctctcgg aagcgttgga aaaattatgg cgccgccact gtcgcctggc tcgcgggtcc
    24301 tgatagccct cattcgggtc tatcaacgcc tgattagtcc gctactcggg ccgcattgtc
    24361 gtttcactcc aacctgttca agctacggaa ttgaggcatt gcgcaggttt ggagtgataa
    24421 aaggcagttg gttgacggtg aaacgcgtat taaaatgcca ccctttacac cctggtggtg
    24481 acgatcccgt cccgcccgga ccatttgata ccagagaaca ctaacgatgg attcgcaacg
    24541 caatctttta gtcatcgctt tgctgttcgt gtctttcatg atctggcaag cctgggagca
    24601 ggataaaaac ccgcaacctc aggcccaaca gaccacgcag acaacgacca ccgcagcggg
    24661 tagcgccgcc gaccagggcg taccggccag tggccagggg aaactgatct cggttaagac
    24721 cgacgtgctt gatctgacca tcaacacccg tggtggtgat gttgagcaag ctctgctgcc
    24781 tgcttacccg aaagagctga actctaccca gccgttccag ctgctggaaa cttcaccgca
    24841 gtttatttat caggcacaga gcggtctgac cggtcgtgat ggcccggata acccggctaa
    24901 cggcccgcgt ccgctgtata acgttgaaaa agacgcttat gtgctggctg aaggtcaaaa
    24961 cgaactgcag gtgccgatga cgtataccga cgcggcaggc aacacgttta ccaaaacgtt
    25021 tgtcctgaaa cgtggtgatt acgctgtcaa cgtcaactac aacgtgcaga acgctggcga
    25081 gaaaccgctg gaaatctcca cctttggtca gttgaagcaa tccatcactc tgccaccgca
    25141 tctcgatacc ggaagcagca acttcgcact gcacaccttc cgcggcgcgg cgtactccac
    25201 gcctgacgag aagtacgaga aatacaagtt cgataccatt gccgataacg aaaacctgaa
    25261 catctcttcg aaaggtggtt gggtggcaat gctgcaacag tatttcgcga cggcgtggat
    25321 cccgcataac gacggtacca acaacttcta taccgctaat ctgggtaacg gcatcgccgc
    25381 tatcggctat aaatctcagc cggtactggt tcagcctggt cagactggcg cgatgaacag
    25441 caccctgtgg gttggcccgg aaatccagga caaaatggca gctgttgctc cgcacctgga
    25501 tctgaccgtt gattacggtt ggttgtggtt catctctcag ccgctgttca aactgctgaa
    25561 atggatccat agctttgtgg gtaactgggg cttctccatt atcatcatca cctttatcgt
    25621 tcgtggcatc atgtacccgc tgaccaaagc gcagtacacc tccatggcga agatgcgtat
    25681 gctgcagccg aagattcagg caatgcgtga gcgtctgggc gatgacaaac agcgtatcag
    25741 ccaggaaatg atggcgctgt acaaagctga gaaggttaac ccgctgggcg gctgcttccc
    25801 gctgctgatc cagatgccaa tcttcctggc gttgtactac atgctgatgg gttccgttga
    25861 actgcgtcag gcaccgtttg cactgtggat ccacgacctg tcggcacagg acccgtacta
    25921 catcctgccg atcctgatgg gcgtaacgat gttcttcatt cagaagatgt cgccgaccac
    25981 agtgaccgac ccgatgcagc agaagatcat gacctttatg ccggtcatct tcaccgtgtt
    26041 cttcctgtgg ttcccgtcag gtctggtgct gtactatatc gtcagcaacc tggtaaccat
    26101 tattcagcag cagctgattt accgtggtct ggaaaaacgt ggcctgcata gccgcgagaa
    26161 gaaaaaatcc tgattcggtg agttttcgct aaaataaggg cggtcagttg accgcctttt
    26221 ttcttttcgt agggtggata agcaccgcgc atccgccaca caaagcaaca ggaacatcat
    26281 gagcgataat gacactatcg tagcccaggc cacgcctccg ggacgtggcg gcgttggcat
    26341 cctgcgcatc tccggcctaa aagcccgtga agttgccgaa accgtgctgg gtaaactgcc
    26401 taagccgcgc tacgccgatt atcttccgtt taaagacgcc gacggcagcg tgctcgatca
    26461 ggggattgcg ctatggttcc ctggcccgaa ctcgttcacc ggcgaagatg tgctggaact
    26521 gcagggtcat ggcggtccgg tgatcctcga cctgctgtta aaacgcattc tgaccattcc
    26581 cggcctgcgg attgctcgcc ctggtgagtt ttccgaacgc gcgtttctta acgataaact
    26641 cgacttagcc caggccgagg cgattgccga tcttatcgac gccagttcgg aacaggcggc
    26701 ccgttcggca cttaactcgc tgcaaggcgc attctccgca cgggtcaacc atctggtgga
    26761 agccctcacc cacttgcgca tttacgtcga agcggcaatt gatttccctg atgaagagat
    26821 cgatttcctc tccgacggca aaattgaagc ccagcttaat gacgtgattg ccgatctcga
    26881 tgcagtgcgt gctgaagcac gtcagggtag tttgttgcgc gaagggatga aggtggtgat
    26941 tgccggacgt cctaacgccg gtaaatcgag cctgttaaac gcgctggcgg gccgtgaagc
    27001 agcaatcgta actgatatcg ccggaaccac acgtgacgtg ctgcgtgagc atatccacat
    27061 tgacggaatg ccgctgcata tcatcgatac cgctgggcta cgtgaagcca gtgacgaagt
    27121 ggaacgtatt ggtatcgagc gcgcgtggca ggaaattgaa caggccgacc gcgtgctgtt
    27181 tatggtcgat ggcaccacaa cagacgccgt ggatccggca gagatctggc cggaatttat
    27241 cgcccgtctg ccagcgaaac taccgatcac cgtggtgcgc aataaagccg atatcaccgg
    27301 cgaaacgctg ggaatgagtg aagtgaacgg tcacgcgtta attcgtctct cggcaaggac
    27361 aggtgaaggc gtggaggtgc tgcgtaacca tctcaaacag agcatgggct ttgacaccaa
    27421 catggaaggc ggcttcctgg cgcgtcgtcg ccacctacag gcgctggaac aggcagcgga
    27481 gcatttgcag caaggtaaag cgcaactgtt aggtgcctgg gcgggtgaac tgctggcgga
    27541 agagttgcgc ctggcacagc agaacttaag cgaaatcacc ggggaattta cctctgatga
    27601 tttgctgggg cggattttct ccagcttctg tattggtaag taaccgcgct tacgaagccg
    27661 tattctgact gtcagatgcg gcttcgct
//
LOCUS       DAZOYL010000019        27459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZOYL010000019 DAZOYL010000000
VERSION     DAZOYL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.019"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..256)
                     /locus_tag="ACHUOY_000598"
     CDS             complement(<1..256)
                     /locus_tag="ACHUOY_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0681477.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVS"
     gene            747..1304
                     /gene="pinE"
                     /locus_tag="ACHUOY_000599"
                     /pseudo
     CDS             747..1304
                     /gene="pinE"
                     /locus_tag="ACHUOY_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(1643..2491)
                     /locus_tag="ACHUOY_000600"
     CDS             complement(1643..2491)
                     /locus_tag="ACHUOY_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR0681478.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            2700..3335
                     /gene="yfhb"
                     /locus_tag="ACHUOY_000601"
     CDS             2700..3335
                     /gene="yfhb"
                     /locus_tag="ACHUOY_000601"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR0681479.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            3393..3896
                     /gene="tadA"
                     /locus_tag="ACHUOY_000602"
     CDS             3393..3896
                     /gene="tadA"
                     /locus_tag="ACHUOY_000602"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR0681480.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(3893..5449)
                     /gene="mltF"
                     /locus_tag="ACHUOY_000603"
     CDS             complement(3893..5449)
                     /gene="mltF"
                     /locus_tag="ACHUOY_000603"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR0681481.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            5707..9594
                     /gene="purL"
                     /locus_tag="ACHUOY_000604"
                     /gene_synonym="purI"
     CDS             5707..9594
                     /gene="purL"
                     /locus_tag="ACHUOY_000604"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR0681482.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10152..11579
                     /gene="qseE"
                     /locus_tag="ACHUOY_000605"
     CDS             10152..11579
                     /gene="qseE"
                     /locus_tag="ACHUOY_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR0681483.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            11744..12457
                     /gene="qseG"
                     /locus_tag="ACHUOY_000606"
     CDS             11744..12457
                     /gene="qseG"
                     /locus_tag="ACHUOY_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR0681484.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            12447..13781
                     /gene="glrR"
                     /locus_tag="ACHUOY_000607"
     CDS             12447..13781
                     /gene="glrR"
                     /locus_tag="ACHUOY_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR0681485.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            13842..14180
                     /gene="glnB"
                     /locus_tag="ACHUOY_000608"
     CDS             13842..14180
                     /gene="glnB"
                     /locus_tag="ACHUOY_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR0681486.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(14225..15415)
                     /gene="hmpA"
                     /locus_tag="ACHUOY_000609"
     CDS             complement(14225..15415)
                     /gene="hmpA"
                     /locus_tag="ACHUOY_000609"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR0681487.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            15743..16996
                     /gene="glyA"
                     /locus_tag="ACHUOY_000610"
     CDS             15743..16996
                     /gene="glyA"
                     /locus_tag="ACHUOY_000610"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR0681488.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17194..18387)
                     /locus_tag="ACHUOY_000611"
                     /pseudo
     CDS             complement(17194..18387)
                     /locus_tag="ACHUOY_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            18505..21782
                     /locus_tag="ACHUOY_000612"
                     /pseudo
     CDS             18505..21782
                     /locus_tag="ACHUOY_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            21879..22862
                     /locus_tag="ACHUOY_000613"
     CDS             21879..22862
                     /locus_tag="ACHUOY_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR0681489.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            22885..24396
                     /locus_tag="ACHUOY_000614"
     CDS             22885..24396
                     /locus_tag="ACHUOY_000614"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR0681490.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWYTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            24421..25419
                     /locus_tag="ACHUOY_000615"
     CDS             24421..25419
                     /locus_tag="ACHUOY_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0681491.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            25452..26546
                     /locus_tag="ACHUOY_000616"
     CDS             25452..26546
                     /locus_tag="ACHUOY_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0681492.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            26558..27430
                     /locus_tag="ACHUOY_000617"
     CDS             26558..27430
                     /locus_tag="ACHUOY_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0681493.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
BASE COUNT         6445 a         6850 c         7512 g         6652 t
ORIGIN      
        1 agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa agcgccaagg
       61 ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt cttaccgcct
      121 cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg gacaaataat
      181 ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata tttctgagca
      241 tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat ttaatgtatc
      301 tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg ttatatcatg
      361 ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct ttttttgatg
      421 ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg agtaagatac
      481 tacaacatct ccaactattt tagccagcct gactacttga cttggtaata tttgttgcta
      541 ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat ttagtaatga
      601 tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat tctactttat
      661 aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac ttttgacgca
      721 gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta tcaacaaatg
      781 accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag ctgatttttg
      841 aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg ctcaggacat
      901 tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc agtatgcggc
      961 atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt agtctgactg
     1021 attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg ggtgccctgg
     1081 ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa gctgctcgcg
     1141 catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg gcgcaggccg
     1201 ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat gatgttggcg
     1261 aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc agacacggcg
     1321 tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca ggccttttgc
     1381 gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg ttctttgtgt
     1441 tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc aataacacat
     1501 tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat cacaattgat
     1561 gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca aaccgggcgg
     1621 gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca ctatgacgaa
     1681 tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc aaatccgtta
     1741 acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt tcttcggcaa
     1801 tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag ccggtaatcg
     1861 ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac tcgcgtcgta
     1921 caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt actgttgcga
     1981 gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc agcttccagg
     2041 caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc cgccgcgcag
     2101 agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt agcgttgcgt
     2161 acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac ggatcatcac
     2221 cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc agcgcttcac
     2281 tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg aacttcacga
     2341 cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa tggcgcgccg
     2401 tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca agcccctggt
     2461 aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa tagtggcgac
     2521 aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgtcgct aattgccatg
     2581 aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt gatgatctat
     2641 ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag gagtatggtt
     2701 tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg catcagcagg
     2761 atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat gcgttacttg
     2821 tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt gcggcacgct
     2881 ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca cgtttacaga
     2941 cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt ccgctggttc
     3001 aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg attaccggct
     3061 ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg cgggttaatc
     3121 ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg cgttgtctgg
     3181 gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg ctgtacagtg
     3241 gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat cgttggcgag
     3301 taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt ccgtgtataa
     3361 tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt agccacgaat
     3421 actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg gaagtgccgg
     3481 tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac cgcccgattg
     3541 gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt ggtctggtga
     3601 tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca tgtgtaatgt
     3661 gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg cgtgacgcga
     3721 aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg aatcaccgag
     3781 tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt gacttctttc
     3841 gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg gattaatttt
     3901 gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc aaatagtttg
     3961 atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac tccgtaggcg
     4021 ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc ttctctttct
     4081 cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca taagcgtagg
     4141 cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc tggctaagta
     4201 aaggcagacg ctgttttacg tcagcccaac tgtcaggatt ccctttggtt tttgccgtca
     4261 gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg gcaaaccaga
     4321 tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc aaataacgca
     4381 cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg ctttgcgcgg
     4441 tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc tgtgcatccc
     4501 agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct tcggcgtatt
     4561 tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa aatgtgcgcg
     4621 tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg cgtgccagcg
     4681 taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg gtattatcgc
     4741 catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc gctacggcga
     4801 gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg gtgtaatcga
     4861 gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg tctaccttcc
     4921 agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc accacgtgac
     4981 ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg cgatactgac
     5041 tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc tgataatttt
     5101 ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta ccattatcga
     5161 ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt acaccgaggt
     5221 aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg ttgatttcgt
     5281 tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct tgaatggcgg
     5341 cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg accgcgagca
     5401 gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat tagttaattc
     5461 tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt ttactcataa
     5521 atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc attggcggca
     5581 cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt cagcgcatca
     5641 gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt agaagacgag
     5701 agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg aatcaacaaa
     5761 ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc cgagtatgtc
     5821 cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact tgaacgcctg
     5881 ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct gctggtgacc
     5941 ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc ccataactgc
     6001 gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga agccggtacg
     6061 ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat gatggaaacg
     6121 gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc gactccggtt
     6181 accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa cctgcgtctt
     6241 ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac aaagcttggt
     6301 cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga gcactgccgc
     6361 cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa atcgctgttc
     6421 aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc ttataaagat
     6481 aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca cgaaacgggc
     6541 cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac tcacaaccac
     6601 ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga aattcgtgat
     6661 gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt ctccgtttcc
     6721 aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa gcctgagcgc
     6781 attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc gtttaacaac
     6841 gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa agtgaacagc
     6901 cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg cgggatcggc
     6961 aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa gctggtcgtt
     7021 ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc catggcgtca
     7081 ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc ggagatggag
     7141 cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa cccaatcctg
     7201 tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact ggtgagcgac
     7261 ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc gggcatgagc
     7321 ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt tgctgccgat
     7381 caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc ggtgattggt
     7441 gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa tcagccgatc
     7501 gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga tgtacaaacg
     7561 ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga cgcggtgaaa
     7621 cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat cggcgaccgc
     7681 agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt gccggtcgct
     7741 aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat ggcgattggc
     7801 gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc ggtcggtgaa
     7861 gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa actttccgcc
     7921 aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga agccgttaaa
     7981 gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg taaagactct
     8041 atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac gtcgccgctg
     8101 tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat cacgccacag
     8161 ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa taacgcgctg
     8221 ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc agatgtacgc
     8281 gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc acagcgtaag
     8341 ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc ggaaatggcc
     8401 tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga tcgcctggcg
     8461 gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga ccgtgaagcg
     8521 gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt agggcaggcg
     8581 gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag cgaaagccgc
     8641 accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct gcgtgacaac
     8701 ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc gggcctgaat
     8761 gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc cactggcgca
     8821 cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga aatggcggca
     8881 gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct gctgaccgga
     8941 cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc ctacggtgat
     9001 gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg tgtacgcgat
     9061 gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg taacggttgc
     9121 cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc acgttttgtg
     9181 cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac ccaaagcccg
     9241 tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc tcatggtgaa
     9301 gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa agggctggtg
     9361 gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc taacccgaac
     9421 ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac cattatgatg
     9481 ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga aaactggggc
     9541 gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg gtaagtcatc
     9601 agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc ataagctccc
     9661 aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac cgtgtcgctt
     9721 tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct taagttgtct
     9781 ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat aaatccctca
     9841 aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag ttactgcata
     9901 atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt gctacataaa
     9961 attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc gtagctatat
    10021 caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa gccgggtaat
    10081 tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc gcgtctgacg
    10141 agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa ctggtaatgc
    10201 tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc tggcaaagcc
    10261 tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt gatgcccggc
    10321 gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt cagtattgcg
    10381 tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt tacagcgaaa
    10441 tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca ttgcgtcagg
    10501 atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct gccgccgccg
    10561 cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg cgcacagtgg
    10621 tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa tattttggtt
    10681 ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc acgcggatga
    10741 ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa gggcgttctc
    10801 tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg caacgtattc
    10861 tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt ttaagacatt
    10921 tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa ttactggctg
    10981 accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt cttgatagca
    11041 gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt aaacaggcgg
    11101 acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca gtggtttctg
    11161 ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc aaagcaacag
    11221 cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac tccaatgcgg
    11281 tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat ggtgcgcggg
    11341 tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc gccatgatct
    11401 tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc agcggtctgg
    11461 gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat ctggtcgacg
    11521 agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac acgaaataat
    11581 gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg cagtgctcga
    11641 aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc cagactgtct
    11701 gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac acatttttca
    11761 acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat tgctgggttg
    11821 cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa ttccggttta
    11881 tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc aaggtaaatc
    11941 aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc gtttaatgcc
    12001 tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa atacctttaa
    12061 acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc agctggtcgc
    12121 gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt atcaactctg
    12181 gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt acagtaaact
    12241 ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg ttctacagca
    12301 gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac gccagctctc
    12361 gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg aaaagcccgc
    12421 gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta ttattggtcg
    12481 atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa ggctacagtg
    12541 tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa aaagtagatt
    12601 tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt gctgaaatcc
    12661 agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct atccccgatg
    12721 ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc gataaagatg
    12781 cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc gatgaacgct
    12841 ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa caggcgcggc
    12901 tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc gggaaagaga
    12961 ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt attgctatta
    13021 actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat gcgcgtggcg
    13081 cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa ggcggaacgc
    13141 tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg ctgcgcgtgt
    13201 tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc aatgtgcgga
    13261 ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa ttccgcgaag
    13321 atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg gagcgcacag
    13381 aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga cataaaccgt
    13441 ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc tggccgggta
    13501 atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca tctccggtga
    13561 ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg ccaacctttg
    13621 ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa atcaccaaag
    13681 gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt tataaactgc
    13741 tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt atgtgttacg
    13801 tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag attgatgcga
    13861 ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc ggtattaccg
    13921 gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc gagctgtacc
    13981 gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt gtcgtaccgg
    14041 acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc ggcaaaatcg
    14101 gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt accggtgaag
    14161 aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat ccggcaacat
    14221 caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc tgcttcacgc
    14281 ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg catagataga
    14341 actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc agaccttcgc
    14401 tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag gtgtgcgcgg
    14461 taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg tgaacatcgc
    14521 cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt gccagcgtgt
    14581 cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc actggtgtgt
    14641 catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg acatcgccaa
    14701 cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt ttcaccgcaa
    14761 tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt tcctgatgcg
    14821 ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat tctgccactg
    14881 cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc ggtgttttag
    14941 ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg ttttcgttat
    15001 agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct ttaccccacg
    15061 cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac aggtgttcac
    15121 cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc gcgatttttt
    15181 ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg gcaatagcgt
    15241 taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa atttctttga
    15301 gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt ggccccgttt
    15361 ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca agcatatggt
    15421 cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa aatagccctg
    15481 caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa aaatttgcat
    15541 cacaaacctg aaaagaaatc cgtttccggt tgcaaactct ttattctcca aagccttgcg
    15601 tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc gcacaatgat
    15661 tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa aattttattg
    15721 ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg attatgatgc
    15781 cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca tcgaactgat
    15841 cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc agctgaccaa
    15901 caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt atgttgatat
    15961 cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact acgctaacgt
    16021 ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc tggaaccagg
    16081 tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg gttctccggt
    16141 taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta ccggtcatat
    16201 cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga ttatcggtgg
    16261 tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg ctgacagcat
    16321 cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg ctggcgtcta
    16381 cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa ccctggcggg
    16441 tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca aaaaactgaa
    16501 ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg ccggtaaagc
    16561 ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc aggtcgcgaa
    16621 aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg tttccggcgg
    16681 cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg gtaaagaagc
    16741 agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac cgaacgatcc
    16801 gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta cgcgtcgcgg
    16861 cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc tggacagcat
    16921 caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct gcgcacgttt
    16981 cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg ttcatgccgg
    17041 atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat attgcaatat
    17101 ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat catcaaggct
    17161 tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact gcgcctcaac
    17221 atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa agccaatttg
    17281 cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg tctggcgaat
    17341 aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc aaatctgatt
    17401 aatattgagg atgttgagga agttatacag actcaaacca atggcattgg cactgcgatc
    17461 aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact gcgctgtagt
    17521 cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata cccgcgcctg
    17581 tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc cacagtcgca
    17641 gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat acaaactgcc
    17701 gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa cgttgatcac
    17761 gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca gcatcacgca
    17821 gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact tcacctcaac
    17881 cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg gatcaacctg
    17941 accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac ggtggcgatg
    18001 ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa tctgtaaata
    18061 ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa tactggtggg
    18121 cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg taccgctact
    18181 atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc tttccagttc
    18241 ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca gcgttgattt
    18301 attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat ggtggcgaat
    18361 ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta attgcgtgat
    18421 tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt taattcagta
    18481 tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga gcgcgttgag
    18541 atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga aaatcgcgtt
    18601 tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct gagcgagcag
    18661 aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa agtgatgatc
    18721 ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca gcgcgatttt
    18781 gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc gtggatttcc
    18841 ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat gcccgttgat
    18901 ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga aacggagccg
    18961 atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg ctggaaatcg
    19021 ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg gccaacccgg
    19081 cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact gcggtgtttg
    19141 atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac tacaaagtgg
    19201 actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca acctcatata
    19261 tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa gatggcggtt
    19321 tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt tggggacaca
    19381 gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg tatatcgaac
    19441 tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat gcttacgaag
    19501 agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg caaaatgcct
    19561 cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg gggctgtatg
    19621 ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa tgcaacgcgt
    19681 tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg ttgcacggta
    19741 tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt gtactgagtt
    19801 atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg ccactggcag
    19861 cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg gagcaatatc
    19921 atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg gacccgctgg
    19981 attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat ttcccgcaag
    20041 cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa aacccgcagt
    20101 gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa tatcaacaag
    20161 ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt ggctattatg
    20221 gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat ttttgccaac
    20281 aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat ctgctgctgg
    20341 tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg cgtgattatc
    20401 cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa tcagccctcg
    20461 cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc gccgggcaac
    20521 tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg gactgtcagc
    20581 gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc ggcgaactgg
    20641 tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg ctggaagaag
    20701 tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg gcctgtttct
    20761 actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc gtagagatgt
    20821 cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat aagcaacacg
    20881 attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg caggatgcac
    20941 gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg gagaaacgac
    21001 tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg accgcagaac
    21061 tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt ctcgccacgc
    21121 gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt atcctcaacc
    21181 agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc tgcgaactgc
    21241 ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg gggcaaactg
    21301 ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat gaaactgaag
    21361 cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc cacagttatt
    21421 acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga ctgctgggcg
    21481 aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa gagatggcga
    21541 aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg tcgctgtatg
    21601 gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg gcgtccgcgg
    21661 gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg attaatccgg
    21721 cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac tacgttcact
    21781 aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca aaataattag
    21841 aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa atgagaacaa
    21901 cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc agggctgcgg
    21961 ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac gctggagtgc
    22021 gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta attgaaacta
    22081 acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg gcgcgtaatg
    22141 tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc gttcgtcgcg
    22201 ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa aagggtgtcg
    22261 ataaatatgt ctcggcgtat ctggtcggcg atccactgga atttggtaaa aaactgggta
    22321 acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt gccgtcatca
    22381 attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa gtattaaaat
    22441 cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta aataaagcga
    22501 tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt atgggggagt
    22561 caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag gccggaaaaa
    22621 ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa aacaatcagg
    22681 tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt ttcgcgcaaa
    22741 cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg ccaatcgatc
    22801 tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat ggtctgccct
    22861 aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc ggtagcaaaa
    22921 gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt taacttcacg
    22981 ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa atcgactctc
    23041 attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat tggcgagacg
    23101 cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg ggttcgtgcg
    23161 gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct ctgccttggt
    23221 caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga tgcccaacgt
    23281 tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac gctaagcccg
    23341 gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg cgtggtcatt
    23401 cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat cagtgcggtg
    23461 aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat ggaagaaatc
    23521 cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg cgatgtgatg
    23581 ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga tcacgttgat
    23641 attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt ccgtgcgtta
    23701 cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga agtgcttggc
    23761 attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt tgggctggag
    23821 acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc cgattacggc
    23881 gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc ggggatcatt
    23941 ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat cagcgccaac
    24001 ggagtgctgc aatggtacac catccgccgc ctgaccgaag aggtgatgca gcggatgacg
    24061 gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa tcagcaaaaa
    24121 gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga cgagccaacg
    24181 cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga gctggctgcc
    24241 gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct ggtgtgtgac
    24301 cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc ggtcaatgtg
    24361 gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag gaacctgaag
    24421 atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa acaatttgtc
    24481 agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta tctggtcttc
    24541 tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct gcgcgatgcc
    24601 gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg tgaaattgat
    24661 gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt gctgcaattt
    24721 gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct gatgggaacg
    24781 ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac actggggctg
    24841 tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt gccgattgac
    24901 gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt atccgcgctg
    24961 atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc cttcgggcgc
    25021 tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat taacgttcgt
    25081 cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac cggcattttg
    25141 ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga gtttgacgtc
    25201 atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc cctgttcggt
    25261 acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct gctcggtatt
    25321 aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt gctggcgaat
    25381 atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc cctggcgcgg
    25441 gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa acgatgctgg
    25501 cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg ccgacgccgg
    25561 ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc gatgtccact
    25621 atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta taccagggat
    25681 ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc tgccgccatt
    25741 ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt tgcccgaact
    25801 gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac ggctggcagc
    25861 gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc ctcctgccgg
    25921 atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg gcctatgaag
    25981 ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt ctggggccgg
    26041 tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc atcggcgttg
    26101 atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat cacggctatt
    26161 tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt ggcgcggatg
    26221 ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa tccaccgctg
    26281 actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag gtcagcgccg
    26341 atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg ttccatatgg
    26401 aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc attacccatc
    26461 gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc aaatgcggga
    26521 aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata ccttatccca
    26581 cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct tctggcgcga
    26641 tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg cctcatgttt
    26701 tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc aggggcgtga
    26761 gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg atggctggct
    26821 gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt atgaacatcg
    26881 cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg atacgctgac
    26941 ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta ccggctggca
    27001 tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg gttactggct
    27061 ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg aactggattt
    27121 taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg gatggaatgg
    27181 tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa cgacgccacc
    27241 tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat atgcgtttga
    27301 tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc cggaaggggg
    27361 tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt tgcgggtaga
    27421 gtggctgtaa tttattaaat gccggataag gcgttcacg
//
LOCUS       DAZOYL010000020        27021 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZOYL010000020 DAZOYL010000000
VERSION     DAZOYL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27021
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.020"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..1380)
                     /locus_tag="ACHUOY_000618"
                     /pseudo
     CDS             complement(1..1380)
                     /locus_tag="ACHUOY_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(1592..1906)
                     /gene="pspE"
                     /locus_tag="ACHUOY_000619"
     CDS             complement(1592..1906)
                     /gene="pspE"
                     /locus_tag="ACHUOY_000619"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR0681494.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1981..2202)
                     /gene="pspD"
                     /locus_tag="ACHUOY_000620"
     CDS             complement(1981..2202)
                     /gene="pspD"
                     /locus_tag="ACHUOY_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR0681495.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(2211..2570)
                     /gene="pspC"
                     /locus_tag="ACHUOY_000621"
     CDS             complement(2211..2570)
                     /gene="pspC"
                     /locus_tag="ACHUOY_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR0681496.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMLDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2570..2794)
                     /gene="pspB"
                     /locus_tag="ACHUOY_000622"
     CDS             complement(2570..2794)
                     /gene="pspB"
                     /locus_tag="ACHUOY_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR0681497.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2848..3516)
                     /gene="pspA"
                     /locus_tag="ACHUOY_000623"
     CDS             complement(2848..3516)
                     /gene="pspA"
                     /locus_tag="ACHUOY_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR0681498.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3683..4660
                     /gene="pspF"
                     /locus_tag="ACHUOY_000624"
     CDS             3683..4660
                     /gene="pspF"
                     /locus_tag="ACHUOY_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR0681499.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4778..6043)
                     /gene="puuE"
                     /locus_tag="ACHUOY_000625"
                     /pseudo
     CDS             complement(4778..6043)
                     /gene="puuE"
                     /locus_tag="ACHUOY_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(6081..7361)
                     /gene="puuB"
                     /locus_tag="ACHUOY_000626"
     CDS             complement(6081..7361)
                     /gene="puuB"
                     /locus_tag="ACHUOY_000626"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR0681500.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(7363..8853)
                     /gene="puuC"
                     /locus_tag="ACHUOY_000627"
                     /pseudo
     CDS             complement(7363..8853)
                     /gene="puuC"
                     /locus_tag="ACHUOY_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(9003..9560)
                     /gene="puuR"
                     /locus_tag="ACHUOY_000628"
     CDS             complement(9003..9560)
                     /gene="puuR"
                     /locus_tag="ACHUOY_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR0681501.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9587..10351)
                     /gene="puuD"
                     /locus_tag="ACHUOY_000629"
     CDS             complement(9587..10351)
                     /gene="puuD"
                     /locus_tag="ACHUOY_000629"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR0681502.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10563..11924
                     /gene="puuA"
                     /locus_tag="ACHUOY_000630"
     CDS             10563..11924
                     /gene="puuA"
                     /locus_tag="ACHUOY_000630"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR0681503.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            12073..12237
                     /locus_tag="ACHUOY_000631"
     CDS             12073..12237
                     /locus_tag="ACHUOY_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681504.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12227..13612
                     /gene="puuP"
                     /locus_tag="ACHUOY_000632"
     CDS             12227..13612
                     /gene="puuP"
                     /locus_tag="ACHUOY_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR0681505.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13746..13991
                     /locus_tag="ACHUOY_000633"
     CDS             13746..13991
                     /locus_tag="ACHUOY_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR0681506.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14304..15947
                     /gene="sapA"
                     /locus_tag="ACHUOY_000634"
     CDS             14304..15947
                     /gene="sapA"
                     /locus_tag="ACHUOY_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR0681507.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15944..16909
                     /gene="sapB"
                     /locus_tag="ACHUOY_000635"
     CDS             15944..16909
                     /gene="sapB"
                     /locus_tag="ACHUOY_000635"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR0681508.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16896..17786
                     /gene="sapC"
                     /locus_tag="ACHUOY_000636"
     CDS             16896..17786
                     /gene="sapC"
                     /locus_tag="ACHUOY_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR0681509.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17786..18778
                     /gene="sapD"
                     /locus_tag="ACHUOY_000637"
     CDS             17786..18778
                     /gene="sapD"
                     /locus_tag="ACHUOY_000637"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR0681510.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18780..19586
                     /gene="sapF"
                     /locus_tag="ACHUOY_000638"
     CDS             18780..19586
                     /gene="sapF"
                     /locus_tag="ACHUOY_000638"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR0681511.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19654..20007
                     /locus_tag="ACHUOY_000639"
     CDS             19654..20007
                     /locus_tag="ACHUOY_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR0681512.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            20375..21163
                     /gene="fabI"
                     /locus_tag="ACHUOY_000640"
     CDS             20375..21163
                     /gene="fabI"
                     /locus_tag="ACHUOY_000640"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR0681513.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21308..22434
                     /locus_tag="ACHUOY_000641"
                     /pseudo
     CDS             21308..22434
                     /locus_tag="ACHUOY_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            22502..24436
                     /gene="rnb"
                     /locus_tag="ACHUOY_000642"
     CDS             22502..24436
                     /gene="rnb"
                     /locus_tag="ACHUOY_000642"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR0681514.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(24514..24617)
                     /locus_tag="ACHUOY_000643"
                     /pseudo
     CDS             complement(24514..24617)
                     /locus_tag="ACHUOY_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24671..26661
                     /gene="pdeR"
                     /locus_tag="ACHUOY_000644"
                     /pseudo
     CDS             24671..26661
                     /gene="pdeR"
                     /locus_tag="ACHUOY_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26643..26771)
                     /gene="ymiD"
                     /locus_tag="ACHUOY_000645"
     CDS             complement(26643..26771)
                     /gene="ymiD"
                     /locus_tag="ACHUOY_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR0681515.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26808..26981
                     /gene="yciZ"
                     /locus_tag="ACHUOY_000646"
     CDS             26808..26981
                     /gene="yciZ"
                     /locus_tag="ACHUOY_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR0681516.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
BASE COUNT         6228 a         7009 c         6975 g         6809 t
ORIGIN      
        1 cagcgttact tctttgaata cgcattacgg atgaattaat cgtatcgatg gtaaaattat
       61 tatccggatg aaactcgtta tggctgcgac gaagtgtaat taaacgcgac aactcatgat
      121 ataccgcatg ccttaatgta gcttcatcgt tcagttctcg ggttatctct ttactgtgat
      181 attttttacg gttaatcgca cggttatatc cgagtttttc gacacctgcg taatcattac
      241 gcgagccaag aatactttga atatatatcg ctggcacacc ggggaaactt aacaaaatcg
      301 catgggcgag aataaacctg gcgcaacgtt cttcatcgct actctcacgg cggcttaacg
      361 catccatatg tgagaggcga ggaagttaaa ccaggtggtg ttgctggaag gtagtgtcag
      421 gttttgcgcc cacgcacaaa gcgcctcaac gttctgtttt tgcaccgcat gcagcaccag
      481 tggcggcagc gagaactggt acaccatatg tgcttcgtca tcgcctgcgc caaagtaagc
      541 aatgttgtct ttatgcggaa cattggtctc ggtaatgatc actgtacctg gcgcaacgtt
      601 atcaataatc gaccgtaaca gtttgataat cagatgtgtt ttttccagat ggatgcagct
      661 tgttcccggc tctttccaca taaagccaac ggcatccagg cggacatact cagcaccttt
      721 cgcaaggtaa cagagtaaaa catccaccat cgccagcaac acttcagggc tacggtaatt
      781 caggtcaatt tgatcgtcac taaaggtggt ccataaatgg cgcgttgaat gatcgcgcat
      841 ctggaatggc gttaataacg gtaacgcacg cgggcgagtg acggcgctga gatcggtttg
      901 cggatcaacc gcaataaaaa aatcttcaaa acctggctgc tgttgtaaat agtttttaaa
      961 ccattcactt tttgccgaca tatggttgca gacaaaatca aacattaaat gactgcattc
     1021 accgagttgc tgaatatcct gccactcccc cgcttcactg gcgacctgat gataatcaat
     1081 taccgaaaac ccatcatcag atgaacatgg ataaaaaggt aataaatgaa catgtgaaaa
     1141 aatgctttgc agccattgat ggtaaaactg attaaatgtg ggtaatggtt ttaaatcatt
     1201 gctgtgaaat tgatcggcat aggtaattaa cacgacatca ctttcatccc agtgtttttt
     1261 acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc gttacaagtt tctgtaaatg
     1321 agttgcggta aatgttccac cgtagatttc gtccaggtaa tccgtaattt tctgtttcat
     1381 gttgcctttt tgtggtagcg gtcccatgaa acgagagact actccgcaga aaataaactg
     1441 tcaattgagc acaagggttt tagtcacgca ggaaatcatt ttttaccagg gaaaaagcgt
     1501 atttgtgagc tgacgcaaat aatctatggg gatgtgaata aggtgtgaca gaaaaaaaac
     1561 ggcgcataag cgccgctcat ggtgaattct tttaaccttt gaccttcggc attgcgatgt
     1621 ctttcaggcc accggcgttc tcaacgtggg tatatcccat ctcgctaagg atctcttttg
     1681 cttgccctga ctggcgtccg gcattgcaat acactttcac ggtgtcgttt ttatccggaa
     1741 cggcggtggc aatgcgctct ttcacttcct tcaggggaat attgatggcc ccctgaacgt
     1801 gctcttgctg atactgctct ggaacacgaa catcgatcca gtgttcagcg gcgaaaacgg
     1861 gcagtgaaaa caccagtgcc agagcaagta agcctttttt aaacatgtaa gcgtccttct
     1921 atggatgaaa attgccatcc attatacgcc tgagatttaa caatttttgc gaaactcccc
     1981 tcacctttta taacgctgtg ccagtttatt agcagcccga cttaacagcg gttccagcgc
     2041 cacagccagc aacattttca gcggtcggcg agcaactgat tttatcgccc agcccgctac
     2101 acccgccggg ccatagcgca gtgcggtaag aagtaccagc ttgcctgcta atttgaaacc
     2161 aggctttacc ttttgcccgg cctgttgcca gcgagtattc ataactttcc tcacagttga
     2221 cggaaacggc tacgtaacgt gaaagtatcg gaagtgacat aacgttccat ctcgcgtaaa
     2281 cgcgtttcac ttgccgccag ttcacggtcg acttcatcca gcaattcgct gctggaaggt
     2341 agctgctcac caaaggccat gttgtccagc attggatcaa gcgcaaatga caaaatgatg
     2401 taagcaacca gggtaaacag cgccagaccg aagaaaatcg acagcaccac caggatacgc
     2461 accagtttta ccggtacatc aaaatagttg gcaatcccgg cgcagacgcc gcggaccatg
     2521 ccctgctgtg gaatacgcca taatttttta ttgagattaa tgcccgccat tagcgatccc
     2581 tccagttcgg atgttctgca tcaagaatag attccagcgc ctgaatacgt tcgcgcatcc
     2641 gttttgcttc atcagccagt tgcgctaatc gctgctgctc actttgcgac aattcatcgc
     2701 gaccagaacg attgctgtaa tgcagccata accagatcgg taaaacaaac agcacaaaaa
     2761 tggttaacgg aatagccaga aatagcgcgc tcataagtac tccttagacg atgagcggcg
     2821 gcgcgttcgg acgccgcctg gatgttatta ttgattgtct tgcttcattt tggcttttaa
     2881 ttgtgccagt tgttcgctga ttgcatcatc ggctttcagt tcggcaaact gatcgtccag
     2941 cgatttttgt ttaccgaagc tgtggctttc tgcttccgct tccatctggt caattcgacg
     3001 ttcgaaagat tcgaaacgag ccattgcttc atccagtttg ccactgtcca gctgacgacg
     3061 cacatcgcgc gacgagtttg ccgcctgatg acgtaacatc aatgcctgct ggcgagcgcg
     3121 tgtttcgctc aatttgtttt ccagctcgcc aatctctttc ttcatgcgtg ccagcgtatc
     3181 gttcaccagc gtcacttcat gttccaggga cttaatcaga tcggtcagtt tctgtttttc
     3241 aattaacgct gcacgtgcca gatcctctct ctctttcagc agcgccagtt cggctttttc
     3301 ctgccattca acttcacgcg ccgacgcttg ttcaatacgg cgagtcagct gtttcttttc
     3361 tgccaacgca cgcgccgaag tagaacgtac ttcaaccagt gtatcttcca tctcctggat
     3421 catcagacgc accagtttct gtggatcttc cgctttctct aacagagcgt tgatgttggc
     3481 attcacgatg tcggcaaagc gagaaaaaat acccataatg ttgtcctctt gatttctgcg
     3541 ttcaggattg tcctgctgaa ctgttaatac aatttgcgtg ccaatttttt atctttttga
     3601 tttataaaga tctgattgaa gaatcaacag caacatgcca ggatgaatta gcgaattaca
     3661 ctaacaagtg gcgaatttca tcatggccga atacaaagat aatttacttg gtgaggcgaa
     3721 cagctttctc gaagtgctgg aacaggtttc gcatctcgca ccgctggaca aaccggtgct
     3781 catcatcggc gaacgcggca ccggtaaaga gctgattgcc agccgcctgc attatctctc
     3841 ctcccgttgg caagggccgt ttatttccct taactgcgcg gcgttaaatg aaaatctgct
     3901 ggattccgaa ctgtttggtc acgaagcggg ggcgtttacc ggtgcgcaaa aacgtcatcc
     3961 agggagattt gaacgtgccg acggcggtac gctatttctt gatgaactcg ctacggcacc
     4021 gatgatggtg caggagaaat tattgcgcgt gattgagtac ggtgaactgg agcgcgttgg
     4081 cggcagccag ccattgcagg tgaatgtgcg gttggtatgc gcgacgaatg ccgatctccc
     4141 ggcgatggtc aatgaaggca cttttcgcgc tgacctgctc gaccgactgg cttttgatgt
     4201 tgtacaactg ccaccactgc gcgagcgcga aagcgacata atgttgatgg cagaacactt
     4261 tgccatccag atgtgtcggg aaatcaagct gcctctgttc ccggggttta cggagcgcgc
     4321 cagagaaaca ttgctgaatt atcgttggcc gggaaatatt cgtgaattga aaaacgcggt
     4381 ggaacgttca gtgtatcgcc acggcaccag cgattatccg cttgatgaca tcattattga
     4441 tccctttaaa cggcgtccgc ctgaagacgc tatcgccgtt tcagaaacca cctcgcttcc
     4501 gacgctgccg ctggatttac gtgagtttca aatgcagcag gaaaaagagt tgctgcaact
     4561 cagtttgcaa aaggggaaat ataaccagaa gcgcgcagct gaattgctgg ggttaaccta
     4621 ccatcagttc cgcgcgttgt tgaaaaaaca ccagatttag cgaagttggg caatgcgatt
     4681 cgccggatgc ggcgtgaacg ctttatccgg catacataat caacgtcgtt tgcaggcctg
     4741 ataagcgcag cgcatcagac attattgcgt tgggctatta atcgctcagc gcatcctgca
     4801 aaattttcat tgccgcatcg aattgcgcat ccgggatggt cagcggatac aggaagcgaa
     4861 tcacgttgcc gtatgcgcca caggtcagca ggagcagccc ctgcgccagc gcgcgttgct
     4921 ggattttctg tgcaatcgcc gctgacggct cgcccgtttg cggatcgtta aactctgccg
     4981 caatcatcga ccccaggccg cgtaccgcag caatggccgg aacgctttct ttggcatcaa
     5041 tcaacgtgtt tttgagacgc tggcccagtt gattcgcgcg ttcgcagagt gattctttgt
     5101 tgataatatt gagcaccgcg tgcgcggcag ccaccgccag cgggttcccg gcgtaggtgc
     5161 cacccaaccc gcccggcgcg ggcgcgtcca taatattcgc gttaccgacc acgcccgaaa
     5221 gcggcatccc gcccgcgagg cttttcgcca tcgtcattaa gtccggctta tcggcgtaat
     5281 gatccatggc aaacagttta ccggtacgcg caaagccgct ttgcacttca tcggcaatca
     5341 tcacaatacc gtgctcgtcg cacaggcggc gaatggcggc aaccagctct tttggcgcaa
     5401 cgttgaaacc gccctcgccc tgcaccggtt cgaaaataat ggccgcgacc tgcttcgctt
     5461 cgatgtctga tttaaacaag cgttcgatgg cgtcgaggga gtcctatgtt gaaatgccgt
     5521 gtaaatctga cggataaggt acgtgataca ccgaaccagg gaacgggccg aagccgattt
     5581 tgtacggcgc aacttttccg gtcagcgcca tggtcatata tgtacgaccg tggaagccac
     5641 cgctaaacgc aatcacgcca gggcgtccgg tatgggcgcg agcaattttc accgcgtttt
     5701 ccaccgcttc cgcaccggta gtgaagaacg cggttttggc ctgcccgctc accggggcaa
     5761 gggcgttgat tttctccgcc agggtgacgt agctttcata cggcacaatc tgatacgcgg
     5821 tgtgggtaaa ctgttgcagt tgctgctcca ccgccgcgac cagatcaggg tggcgatgtc
     5881 cggtattcag caccgcaatg cctgcggcga aatcgatgta ctcgttgccc tcaacatcct
     5941 tcagagtagc gttttcagcc gactgggcga agaagttaca catcacgcca accccgcgtg
     6001 gggtagcaga aagacgacgc tgatggaatt cattgttgct catgtttcac accttttgac
     6061 tcttcactgt taatcggaaa ttaaacgccc agacgatcgc gcaggctgta ccaggctgcg
     6121 cccatcgcgg taaacggtac tcgcagcgtg cgcccgccgg ggaacgggta atacggcaga
     6181 ttggcgaagg catcgaaacg ttcggcgtcg ccgcgcagca gttcggcaat caaacgtccg
     6241 gcgagatgag tacaggtgac gccgtggccg ctgtaaccct gcatgtaata gatgttggta
     6301 tcaaggcgac caaactgcgg catgcgcgac agggtcagca ggaagttgcc cgtccagcgg
     6361 tagtcgattt tgacgccctt cagttgcggg aaggttttca gcagtttcgg cacaacaaga
     6421 cgctcaacgt catccgggtc gcgcgcgccg tagaccacgc cgccgccgta cagcaaacgg
     6481 ttgtcggcgg taagacggta gtaatcaagc agataattac agtcttccac acagtagttt
     6541 ttcgggatca gcgaacgggc taaatcttcc gacagccgtt cagtggtgat cacctgcgtg
     6601 ccgcacggca tgctgcgttt cgccagttcc ggctctactt tatcgcccag atacgcattc
     6661 ccggcgacaa tcacatactt cgccgtcacc tgacctttgg cagtgcgcac caccgctggc
     6721 gtggtgtgct ggatctgcgt cacggcagaa agctcataca cgcgcccgcc gttcaggcgg
     6781 atggcatcgg cttcaccaat ggcgaggttt agcgggtgaa tatgcccacc gctgtgatcc
     6841 aacagtgcgc cagtgtagcg atcgctggcg acctcgcggc gaatggcgtt agcgtccagc
     6901 agctccagct gtttattgcc gtagcgttcc cagttctctt tctgctcttc gagtgtggcg
     6961 agctgtttat cattcatcgc cacaaacagg ccgccggggc ggtagtcgca gtcaatctga
     7021 tagcgtttga tacgttcgcg gatgatctcg ccaccctcga acatcatgct gccgagcata
     7081 cgggcggtgt ccatgccgta gcttttttcg atcacgtcga tgtcgcggct gtaggagttc
     7141 acaagctgcc cgccattgcg cccgcttgcg ccaaagccga tgcgtgaggc ttcgagaacc
     7201 actacgtcaa agcccgcttc cgccagatgc agcgcggagg agagcccggt atagccgccg
     7261 ccaaccacgc aaacgtcgca ggtgattgac tcattcagtg tgtcgaatgg tgcatattta
     7321 ttcgcactgg cggcgtagta actgctggta tgttcggtca tttcaggcct ccaggcttat
     7381 ccagatggtt ttcagttcag tgaatttttc aagggcatgc agggatttgt cgcgaccgtt
     7441 gccgctctgc ttatagccgc caaacggcac ggtcatatcg ccgtcgttgt agttattgac
     7501 gaagacggaa ccggctttca ggcggcggct catacggtgc gcgcgggaga ggtcgcgcgt
     7561 ccataccgcc gcgccaaggc cgtactggct gtcgttggca agctgtagcg cctggtcttc
     7621 tgatgtgaaa cgcgtgacca ccagcaccgg accgaaaatc tcttcgcggc ttaaggacgc
     7681 attcgggtca acatccacaa agatggtcgg gccgatggcg gcagccagct cagcgttacg
     7741 accatccagc agcagttgcc ctttgctttc gccttcacga ataaagctat ggaccgagtc
     7801 ggcgtgggcg caatcgatta aggtgcccat ggtggttacg gtatcaagtg gatggcccgg
     7861 ctgccagttt tgcgcctgct gttttaacag ggctaagaat tcatcggcga tgctctcttc
     7921 cagtaacaaa cgcgttccgg cgatgcacac ctggccctgg ttgtagaaga tgcctgcggc
     7981 ggtggcgctt gccgcttttt gcagatccgg acaatcggcg aaaacgatgt tggcgctttt
     8041 gccgcccgct tccagccaga cgcgtttcat gttgctatca cccgcgtctt tcagcagttg
     8101 tttacccgta cgcgtggagc cggtaaaggc gatggcatcg atatcgttat gacgcgaaag
     8161 cgcctgcccg gcttcatgac cataaccgct cactacgttc agcacgccat ccggcagccc
     8221 cgcttcttct tttgccagcc cggcgagacg aatcgcactg agtggtgatt tttcagacgg
     8281 ttttagaatc acgctgtttc ccgccgccag cgccgggccg agtttccagc aagtcagcag
     8341 cagcgggaag ttccacggca cgatggcggc aatcacgccg accggttcac gcacgatcat
     8401 cgccagctca tggctactgg tggtcgccac ttcgccatac actttgtcga tcgcttcggc
     8461 gtaccagcga atggcgcgcg ccgcgccggg aatatcatca cgcagactgt gacgaatcgg
     8521 tttgccggtg tcgagagttt ccagcagtgc cagctcttcg gcgtgggctt ccattaaatc
     8581 ggcgagttta ttcagtaccg ctttacgttt agccggagaa gagagtgacc agtcgccgcg
     8641 ttcaaatacg ccgcgtgctg cgctcaccgc atggtcgata tcgacgctct tgccgcgggc
     8701 aattttcgcc agcggtgcct aggtgaccgg atcaacggtt tcaaaggttt cattttccgc
     8761 cgcagcagta tattcaccgt taataaataa gcggttttca atggcgagac ttaacgcttt
     8821 atcctgccag taagccagat gatgaaaatt cattatgact cctgtttcac gtctatcaga
     8881 tatatgcagt ttgtgaacgt cagggcgcag agatccccgg gccgcatgcc cgtttatttc
     8941 tggcgacatt ctttatggat tgccgggcgc attgcgctta cccggcttac agaacaaaaa
     9001 gattaaaacg tggtgggcgt atgggcgctg ataattcggc aaatacctgc cgacgtatta
     9061 ctgaaactgt gcgggatgcc ggtattaatg gcataacttt gccccgcgac gaggtggtaa
     9121 tcctgaccat taatcgtcag aacaatttca ccttccagta ctgtgcctat ttcctcaccc
     9181 tgatgcttaa ttctttcccc agtggttgtg cccggctggt acgtttcaaa gatcatcgcc
     9241 agcgtgcgat tcgggttacc gttatgaacc agcttcattg acacaccctg actgcccatc
     9301 tcaattaagt cgtcctgatt aatgacgacc tgcggctcat caggtttttc cggctcggaa
     9361 aagaattccg agagtgacag accatacacc ttcagcagct tttgcagcgt actgatggca
     9421 gggctgactt tatcttgttc tatcgtactg atagcactgt gagtcagccc ggagagttcg
     9481 gcggcacgac gttgtgaaag cccctgctgc tggcggattt ccgacaagcg ttttcctggc
     9541 gccagtccct catcactcat atttgcattt ccttaaactg tagtggtcag agtcgctgtt
     9601 tttcagcgat atggtgctga caagcggtga taaagccctc gaacaatata cgcgaaagcg
     9661 cgtactcgct actgttccat tccgggtgcc actgtacgcc cagcgcaaaa ggatgattga
     9721 tgacgctaac cgcctcaact aagccatccg gcgagcgagc ttcaacacgc aaccgtgggc
     9781 taacgacctt cgccccttgt ccatgtagag agtttaccca aaagttgcta cattcaggta
     9841 acaacgcaga cagtaatccc ccctcttcaa cctgaacttc atgcgacggt gcatattgct
     9901 gttccaccgg cagttcggga tcttcccgat gttccagcaa ttcaggctgt tcgcacagct
     9961 tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg taaaccccgg cagatggcga
    10021 aaatggggat gcgcctttcg agtgcggcat taattatcgc catgctcaga agatcacgcc
    10081 cgggatcggc gtcaggctca tcgccgtttt caccatatag gtgcggctgc acattgctgg
    10141 gactaccagg aagataaatg ccatcgagtt tcggcaaaag ttgttcaagt aacgacggtt
    10201 ccgccagcgc atgtggtagc gcaattggca agccgcctgc gtggatgatg gcattcaggt
    10261 acttttcttg cagagtctgg gtcgcatgac ccttaagcct gttcctgcac attacgacac
    10321 cgataaccgg attgttcatt atattttcca tgctcaatct cacaaagtgg actaaattat
    10381 cgccattact gcgctttaaa tgacctttat gttcaatatt ttttcaatct agcagtgggt
    10441 tttcattttt gcaaactcaa tttaacattt gacaaacatt tagtttgcat acagattcga
    10501 atggtggtca ttatatttta cgctttgata acgagcggaa aacaaaccaa aggcgaagaa
    10561 tcatggaaac caatatcgtt gaagtagaga actttgttca gcagtcagaa gagaggcggg
    10621 gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg ctacccgaat acgcaatatg
    10681 ttgatgtttt acttactgat ttgaatggtt gcttccgtgg aaaacgtatc cctgtttcaa
    10741 gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc ggtatttgca atggatattc
    10801 tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat gggcgagccg gatcgtacct
    10861 gtgttcctgt tctcggttcc ttaactcctt ctgccgccga tccagagttt atcggtcaga
    10921 tgctcctgac catggtcgat gaagatggcg ctccctttga cgttgagccg cggaacgttc
    10981 ttaaccgcct gtggcagcag ctgcgccagc gcgacgctga agggaatctg caaccgccct
    11041 gcgcgcctgg caccgatgac cgcaatacac aaagccaggt ttactccgtt gataacctca
    11101 accacttcgc cgacgtgctc aatgatattg atgaactggc gcagttgcag ctgattccgg
    11161 cagatggcgc ggtcgctgag gcctcgccgg gtcagtttga aatcaacctt taccatactg
    11221 ataacgtgct ggaagcctgc gatgatgcgc tggcactaaa acgcctggtg cgtctgatgg
    11281 caggaaagca taagatgcac gccactttta tggcgaagcc gtatgaagag cacgcgggca
    11341 gcgggatgca tatccatatc agtatgcaaa ataatcgtgg cgagaatgtg ctttctgaca
    11401 cagaaggcga agattcgccg ctgctgaaaa agatgctcgc cgggatgatt gacctgatgc
    11461 cgtcgtcgat ggcgttgctg gcaccaaacg tgaactcgta tcgccgcttc cagccgggaa
    11521 tgtatgtgcc gacgcaggcg tcgtggggcc ataacaaccg caccgtcgcc ctgcgtattc
    11581 cgtgcggcga ccgtcataat caccgcgtgg aatatcgcgt ggcgggtgcc gatgccaacc
    11641 catatctggt gatggcagcg atttttgccg gtattttgca tggccttgat aacgagctgc
    11701 cgttgcagga agaagtcgaa ggcaacgggc tggaacagga aggcttaccc ttcccgattc
    11761 gccagagcga tgccctgggt gagtttatcg agaatgatca cctgcgccgc tatttaggca
    11821 aacggttttg ccatgtgtat cacgcctgta aaaatgatga gctgttgcag tttgagcgcc
    11881 tcatcacaga aacagaaatt gagtggatgt tgaaaaacgc gtaattcccc ttgcggggtt
    11941 gtttcatcgg cctgagcgga gccaggcctg acatttgctg gtaatccaga catttcccgg
    12001 atgtcgcgtt atggagcgca ggcggcaacg ggcttccgtt aacgattaag actgtgacga
    12061 ggaaatgaga tgatgccgat gattaaatcc ccgcatggcg agggaggctg cgtatgcgcc
    12121 cctcctgcta ctgactggac tccgccccct ttgcttccct tgctaaacag gtttgatttc
    12181 aggtcgacac gaccgcaaac cttattacgc aggggaggca gcaattatgg ctattaattc
    12241 accactgaat attgctgcgc aacccggcaa aacccgtctg cgaaaatcac tgaaattgtg
    12301 gcaggtggta atgatgggtc tggcctatct cacgccgatg accgtatttg atacttttgg
    12361 tattgtctcc ggcattagcg acggtcacgt tccggcgtcc tatttgctgg cgctggcggg
    12421 cgtgctgttt accgctatca gctacggcaa actggttcgc cagtttccgg aggccggttc
    12481 ggcctatacc tacgcgcaaa agtcgattaa cccgcacgtc ggatttatgg tcggctggtc
    12541 atcactgctg gattatctct ttttgccaat gatcaacgtt ttgttggcga aaatctatct
    12601 ctccgccctc ttcccggaag tgccgccgtg ggtgtgggtg gtaaccttcg tcgccatttt
    12661 aaccgccgcg aatctgaaga gcgtcaacct ggtcgctaac ttcaataccc tgtttgtact
    12721 ggtgcaaatc tccatcatgg tggtgtttat cttcctggtg gttcagggac tgcataaagg
    12781 agaaggcgtt ggcaccgtct ggtcacttca gccgtttatc agcgagaacg cgcacctgat
    12841 cccgattatt accggtgcga cgattgtctg tttctcgttc ctcggtttcg atgcggtgac
    12901 cacgctttcg gaagagacgc cggacgccgc acgcgtgatc ccgaaagcca tcttcctgac
    12961 ggcggtatac ggcggcgtta tctttatcgc ggcgtcgttc tttatgcagc tgttctttcc
    13021 cgatatcagc cgctttaaag acccggacgc cgcactgcct gaaattgcgc tgtacgtcgg
    13081 cggcaagctc tttcagtcga ttttcctctg caccacgttt gtgaacacat tagcgtctgg
    13141 cctggcttca catgccagcg tgtcgcgtct gctgtatgtg atggggcgtg acaatgtgtt
    13201 tccggagcgc gtgtttggct atgtgcaccc aaaatggcgg actccggcac tcaacgtcat
    13261 tatggtcggg attgtcgcgc tgtcggcgct gttctttgat ttagtcaccg cgacagcact
    13321 gattaacttc ggtgctctgg tggcgtttac cttcgtgaat ctgtcggtgt ttaatcattt
    13381 ctggcggcgt aaaggaatga ataaaagctg gaaggatcac ttccactatt tgctgatgcc
    13441 gctggttggc gcgctgacgg tgggtgtgct gtgggttaac ctagagtcaa cgtcactgac
    13501 actcggtctg gtatgggctt cgttaggcgg cgcatatttg tggtatctga tccgccgcta
    13561 tcgcaaagtg ccgctgtacg aaggtgacag aacgccggtg agcgaaacgt aatagccgga
    13621 tgcgcggcgc attatcctgc ctgagggtaa cgcgccgcgc cattaactat aaaaagatgt
    13681 ataacccgga tcacgagtgc tatcatccgc ccttatgctt agcgcattaa ccccaggggc
    13741 aaataatgaa ccacgatatc ccgctaaaat attttgatat tgccgatgag tacgcgaccg
    13801 aatgcgcaga gcctgtcgca gaggctgaac gcacaccgct ggcccactac ttccagttac
    13861 tgctcacccg tttgatgaac aatgaagaga tcagcgaaga agcccaacat gaaatggctg
    13921 ctgaagcggg gattaatccc gtacgcattg atgagatcgc agagtttttg aatcaatggg
    13981 gtaatgagta attatttaat tgcagacgat cggtcagtat cgttgtccgg ataaagcgtt
    14041 cacgccgcat ccggctaaca atgacacgca ctgcagcgtg ctttaatggg tttctcacga
    14101 tcgtaccgct ctgctcctcg ctaagttgtt ctgtgagtta tagttataga gaaatcacgg
    14161 gtacgaatgt tgaacagatg gcgacgccac ctgttcaacg accaaccaca cggccctcat
    14221 tacgacccct gaacacaaac ggtaatggca aatgtcatta aatattagta agatagcctc
    14281 tttacataaa atcccttaat attatgcgcc aggtattatc gtctcttttg gtgattgctg
    14341 gacttgtgag tggtcaggca atcgccgcgc ctgaatcccc cccgcatgct gatatccgcg
    14401 acagcggttt tgtctattgc gtcagcgggc aagtcaacac ctttaaccca tccaaagcga
    14461 gcagtgggtt aattgtcgat acccttgccg cccagtttta tgatcgactg ctggatgtcg
    14521 atccctatac ctatcgcctg atgccggaac ttgccgaaag ctgggaagta ctcgacaacg
    14581 gcgcgaccta tcgcttccac ctgcgtcgcg atgttccgtt tcaaaaaacc gactggttta
    14641 ctcccactcg taaaatgaat gccgacgatg tggtgtttac cttccagcga atttttgacc
    14701 gcaacaaccc gtggcataac gtcaacggca gcaacttccc ctacttcgac agcctgcaat
    14761 ttgccgataa cgtgaaaagc gtccgcaaac tggataatca taccgttgag ttccgactcg
    14821 cccagccgga tgcttctttt ttgtggcacc tcgcaaccca ttatgcttcg gtcatgtcgg
    14881 cagaatatgc ccggaagtta gagaaagaag atcgccagga gcaactcgac cgtcaaccgg
    14941 tcggcaccgg tccgtatcag ttgtcggaat accgcgccgg gcaatttatt cgcctacaac
    15001 gtcatgatga cttctggcgc ggtaaaccgt taatgccgca ggtagtggtg gatttaggct
    15061 ccggcggcac cggacgtctg tcgaaactcc tgaccgggga atgcgacgtt ctggcctggc
    15121 ctgctgccag ccagctatcc attttgcgtg acgacccgcg cttgcgttta acgctgcgtc
    15181 ctgggatgaa cgtcgcctat ctggcattta acaccgccaa accgccgcta aataatcccg
    15241 ctgtccgcca tgcactggca ctggcgatta ataaccagcg cctgatgcaa tccatctatt
    15301 atggtacggc tgaaacggcg gcctctattt taccgcgcgc ctcgtgggcc tatgacaacg
    15361 aggctaaaat tactgaatac aatccggcga aatcgcgcga acagttgaag gcgttggggc
    15421 tggaaaactt aacgctgaaa ctgtgggtgc ccacacgttc gcaggcgtgg aaccccagtc
    15481 cactgaaaac tgccgaactg attcaggcgg atatggcgca ggttggcgta aaagtggtga
    15541 ttgtgccggt agaaggtcgc tttcaggagg cgcggttgat ggatatgagc catgatctga
    15601 cattatccgg ttgggcgacg gacagtaacg acccggacag tttcttccgt cctttactga
    15661 gctgcgcggc aattcattca cagaccaacc tcgcccactg gtgcgatccg aaattcgaca
    15721 gcgtgttgcg taaggcgctc tcctcgcagc agctggcggc gcgtattgaa gcctatgacg
    15781 aagcgcagag tattctggcg caggaattac ctattctgcc gctggcgtcg tcattgcgtt
    15841 tgcaggccta tcggtacgat atcaaaggtc tggtacttag cccgtttggt aacgcctcct
    15901 ttgctggggt gtatcgcgag aaacaggatg aggtgaaaaa accatgatta tcttcacctt
    15961 acgccgcatt ttgttattga ttgtcacctt gttcctgctg accttcgttg gcttcagctt
    16021 gagctatttc acccctcatg cgccgttaca aggcgcgtca ctgtggaatg cctgggtgtt
    16081 ctggtttaac ggcctgatcc actgggattt tggtgtttcc agcattaacg gtcaaccgat
    16141 tgccgaacag ttaaaagagg tcttcccggc gacgatggaa ctgtgcatcc tcgccttcgg
    16201 ttttgcgctg attgttggca tccccgtggg gatgattgcc gggattacgc gccataagtg
    16261 gcaggataac ttgatcaacg ccatcgcctt actgggcttt tcaatcccgg tgttctggct
    16321 ggcgcttctg ttgacgctgt tttgttcact cacgctaggt tggttgcccg tttcagggcg
    16381 tttcgatctg ctctacgaag tgaaaccgat taccggtttt gcgttgattg atgcctggct
    16441 ttcggactca ccatggcggg atgaaatgat catgagcgca atccgccata tgatattgcc
    16501 cgtgatcacc ctgtcggttg cgccaacaac tgaagtgatc cgcctgatgc gtatcagcac
    16561 tatcgaagtg tatgaccaga actatgtaaa agcggcggcg acccgcggtt tgtcgcgctt
    16621 tactattttg cgtcgccacg ttctgcataa cgcgttaccg ccggttattc ctcgcctggg
    16681 cttacagttt tccaccatgc tgacgctggc gatgatcacc gaaatggttt ttagctggcc
    16741 gggtttaggg cgctggttaa ttaacgccat ccgccagcag gattacgcag ccatttccgc
    16801 cggagtgatg gtgtgtggct cactggtcat tattgttaac gtgatttctg atattttggg
    16861 tgccatggct aaccctctga aacataagga atggtatgcc ttacgatagc gtatacagcg
    16921 aaaagcgccc gccgggcacg ctgcgtaccg cctggcgcaa attttatagt gacgcctctg
    16981 cgatggtcgg cctgtacggc tgcgcggggc tggctgtact gtgtattttt ggcggctggt
    17041 ttgcgcctta cggcatcgac cagcaatttc tcggttatca attattgccg ccatcatggt
    17101 cgcgctatgg cgaagtttct ttcttcctgg ggactgacga cctcggacgc gatgttttaa
    17161 gccgtttact gagcggagct gcgccaaccg tgggtggcgc atttgtggtg acgcttgccg
    17221 cgacgatctg tggcctggta ctgggcacct tcgccggggc gacacacggc ctccgctcag
    17281 cggtgcttaa ccatattctg gataccttac tggcgatccc ttcgctgctg ttggcaatta
    17341 tcgttgtagc gtttgccgga ccgagtttgt cccacgctat gtttgctgtc tggctggcat
    17401 tgctgccgcg tatggtacgt tcgatttaca gcatggtgca tgacgaactg gaaaaagagt
    17461 acgttatcgc cgcccgtctg gatggcgcat caacgctgaa tattctctgg tttgctgtga
    17521 tgccaaacat caccgccggg ctggtcaccg agatcacccg cgcactgtcg atggcaattc
    17581 tcgatatcgc cgcgctgggc tttctcgatc tcggcgcaca actcccctcg cctgaatggg
    17641 gagcgatgct cggtgatgcg ctggaactga tttatgtcgc cccgtggact gtcatgctgc
    17701 caggtgcggc aattatgatt agcgtgttgt tggttaacct ccttggcgat ggcgttcgtc
    17761 gtgcgattat tgcgggggtg gaataatgcc attactcgat attcgtaacc tgaccattga
    17821 atttaaaacc ggtgatgagt gggttaaagc cgtcgaccgg gtaagtatga ccttaaccga
    17881 aggtgaaatc cgcggtcttg ttggtgaatc cggttccggc aagagtttga ttgcgaaagc
    17941 gatttgtggg gtgaataaag ataactggcg tgttactgct gaccgtatgc gttttgatga
    18001 tatcgatttg ctgcgtctct ccgcacgcga acggcgcaaa ctggttggcc ataacgtgtc
    18061 gatgattttc caggaaccgc agtcgtgtct tgacccttca gaacgtgtgg gtcgccagtt
    18121 gatgcaaaac atcccagcct ggacctataa aggccgttgg tggcagcgtt ttggctggcg
    18181 caaacgccgt gccattgaac tgctgcaccg cgtggggatt aaagatcaca aagatgcgat
    18241 gcgcagtttt ccctatgagt tgaccgaagg tgaatgtcag aaagtgatga tagccattgc
    18301 actggcgaat caaccgcgtc tgctgattgc tgacgaaccg accaactcaa tggagccaac
    18361 aacccaggcg caaatctttc gcctgctgac gcgtctcaac caaaacagca ataccactat
    18421 tttgcttatc agccatgact tacaaatgct tagccaatgg gcggataaaa ttaacgtgct
    18481 ttactgcggt caaacagtgg aaaccgcgcc gagtaaggag ttggtgacga tgccacatca
    18541 tccttatacc caggcgctga tccgcgcgat accagacttc ggcagcgcga tgccgcataa
    18601 aagtcgcctc aatacgctgc ccggcgctat cccactgctg gaacagttac cgattggttg
    18661 tcgtctgggg ccacgttgcc cgtatgcaca acgagaatgc attgtgacgc cacgtttgac
    18721 gggggcgaaa aatcatctct atgcctgtca tttcccgctg aacatggaga aagagtgaga
    18781 tgatcgaaac gctgcttgaa gtgcgtaatt taagtaaaac gttccgctac cggaccggct
    18841 ggtttcgtcg tcagaccgta gaagcggtaa aacccttgag ctttacgcta cgtgaaggcc
    18901 agacactggc gattattggc gagaatggtt cgggtaaatc cacgctggca aaaatgctgg
    18961 cgggaatgat agagcctacc agcggcgagt tattgattga cgatcatcca ctgcattttg
    19021 gcgattattc cttccgcagt cagcgtattc gcatgatttt tcaggatcct tcgacctcgt
    19081 tgaatccccg tcaacgtatc tcgcaaattc tggattttcc actgcgcctg aacaccgacc
    19141 tggaaccgga acagcgtcgc aaacagatta ttgaaacgat gcgtatggtt gggctgctac
    19201 cggatcacgt cagttactat ccgcatatgt tggcacccgg acaaaaacag cgtctgggtc
    19261 tggcgcgcgc gctaatattg cgcccaaaag tcattattgc cgatgaagcc ctcgcttcac
    19321 tggatatgtc gatgcgttcg cagttgatta atctgatgct ggagttacag gaaaaacagg
    19381 gcatttcgta tatttatgtt acccagcata tcggaatgat gaagcacatt agcgaccagg
    19441 tgctggtgat gcatcagggc gaggttgtcg agcgaggcag caccgcggat gtgctggcat
    19501 cgccgctgca tgaactcacc aaacggctga ttgccgggca ctttggcgaa gcattgacag
    19561 cggatgcgtg gcgtaaagat cgctaatttt ctgaatcctc cttccagatt atccacctca
    19621 ttctgacaag agattaaatc ttgtcagaat gaggtgatgg ataaaataaa atcaaatgcg
    19681 cgtgatttaa gacgcaatct cactttgcag gaacgaaagc tctggcgata tcttcgcagc
    19741 cgacgttttg gtgatttcaa atttcgccgt caacatccgg tggggagcta cattctcgat
    19801 tttgcttgct gctcggcgcg cgtagtcgtt gagctggatg gtgggcagca tgatttggca
    19861 gttgcctatg ataccaggcg cactagctgg cttgagtcgc agggctggac cgtgctgcgt
    19921 ttctggaata acgagattga ttgtaatgag gaggcggtgc cggagattat tctgcaggaa
    19981 ctgaaccgcc gatcaccctc accctgaaag ggcgaggggg ccgaccgagc cgaatagctg
    20041 ttgtggtgaa aacatggaga cagtgctgga gaatattcgg caaggtctga accatcccag
    20101 ccatcgccat aaaagggtta ggggctgtat gagcctgttt gttgctgggg taacaatatt
    20161 tgcacaatat ggtcccctcg cccctctggg gagagggtta gggtaagggg aaaagcgccc
    20221 accctgccgc agcctgctcc ggtcggacct ggcaactata gctactcaca gccaggttga
    20281 ttataataac cgtttatctg ttcgtactgt ttactaaaac gacgaatcgc ctgattttca
    20341 ggcacaacaa gcatcaacaa taaggattaa agctatgggt tttctttccg gtaagcgcat
    20401 tctggtaacc ggtgttgcca gcaaactatc catcgcctac ggtatcgctc aggcgatgca
    20461 ccgcgaagga gctgaactgg cattcaccta ccagaacgac aaactgaaag gccgcgtaga
    20521 agaatttgcc gctcaattgg gttctgacat cgttctgcag tgcgatgttg cagaagatgc
    20581 cagcatcgac accatgttcg ctgaactggg gaaagtttgg ccgaaatttg acggtttcgt
    20641 acactctatt ggttttgcac ctggcgatca gctggatggt gactatgtta acgccgttac
    20701 ccgtgaaggc ttcaaaattg cccacgacat cagctcctac agcttcgttg caatggcaaa
    20761 agcttgccgc tccatgctga atccgggttc tgccctgctg accctttcct accttggcgc
    20821 tgagcgcgct atcccgaact acaacgttat gggtctggca aaagcgtctc tggaagcgaa
    20881 cgtgcgctat atggcgaacg cgatgggtcc ggaaggtgtg cgtgttaacg ccatctctgc
    20941 tggtccgatc cgtactctgg cggcttccgg tatcaaagac ttccgcaaaa tgctggctca
    21001 ttgcgaagcc gttaccccga ttcgccgtac cgttactatt gaagatgtgg gtaactccgc
    21061 ggcattcctg tgctccgatc tctctgccgg tatctccggt gaagtggtcc acgttgacgg
    21121 cggtttcagc atcgctgcaa tgaacgaact cgaattgaaa taatcattct gttggtaaat
    21181 attgggcggc gttctgccgc ccgttatctc tgttatacct ttctgatatt tgttatcgcc
    21241 gatccgtctt tctccccttc ccgccttgcg tcaggataac gatttccttt acgaccaagg
    21301 agcgcccatg gaacaacgcc acatcaccgg caaaagccac tggtatcatg aaacgcaatc
    21361 cagtactgcg gagtatgacg ttctgcctct ggtcccggaa gccgcaaagg tcagcgatcc
    21421 ctttctgctc gacgtgatcc ttgatgaaga aacgctggct cccttccttt catggctggt
    21481 ccctgcgcgc gttcttgcag tggaattgtt ccctgaccag cttaccgtga cccgttcaca
    21541 gaccttcacc gcttatgaac gcttgtctac ggccctgacg gttgctcagg tttgcggcgt
    21601 ccagcggtta tgtaactact attcggcgcg acttacgccg ctccccgggc ctgattcctc
    21661 cagggaaagc aatcataggt tggcacaaat cacgcaatat gcccgccaac tggctagctc
    21721 gccttctatt atcgacaacc gatcgcgcca gcatctgaat gacgtcggtc ttactgcctg
    21781 ggactgtgtg atcattaacc aaatcattgg ttttattggc tttcaggcgc ggaccattgc
    21841 gacatttcag gcttatctcg ggcacccggt acgctggtta cccggtctgg aaatacaaaa
    21901 ctacgccgac gcgtcactgt ttgctgatga atcaatacgc tggcgaagca gctatgaggt
    21961 ggaaaaacta cctgaagagt acacaaaaag ttcaacagca gaactttgcc aactggccga
    22021 aacactctct ctccacccta tttcactttc ccttctcgaa aggttgttaa acagtacacg
    22081 ggttaataca cagccggata ataagcttgc ggcgttgtta tgcgcgcgga taaatggcag
    22141 tcctgcttgt tttgccgcct gtatggattc atcaaatgaa tataaaaaat cagccccctt
    22201 ctgcgcaagg gcgaaaatga aattaaccaa tgggctgacc gtcattctgt tgagcacgct
    22261 accgttcagg cgatacaatg gctgacccga gcacccgatc gctttagcgc cgcccagttc
    22321 agccctttac tcggacacga aaaatcatca acgcagatta ttaatctgct ggtatggagc
    22381 gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg gtgagacata ttaaccttgc
    22441 cgcgtcagac agattcgcgt aaaactgtca gccgctctaa tggccaccaa aatagacaat
    22501 tatgtttcag gacaacccgc tgctagcgca gcttaaacag caactgcatt cccagacgcc
    22561 acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt ggcttcctgg aagtcgacgc
    22621 gaaaaaaagt tatttcattc cgccgccgca gatgaaaaaa gtcatgcatg gcgaccgaat
    22681 tatcgcggtg atccacagtg aaaaagaacg tgaatccgca gagccagaag aactggttga
    22741 accgttcctg actcgtttcg tgggtaaagt tcagggcaaa aatgaccgtc tggccatcgt
    22801 tcctgatcat ccactcttaa aagacgccat tccttgccgc gcagcccgtg gcctgaacca
    22861 cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc cgtcatccgc tgaaaggcga
    22921 tcgttctttc tatgctgaac tgacacaata catcactttt ggtgacgacc actttgtacc
    22981 gtggtgggtt acccttgcgc gccataatct ggaaaaagaa gcaccagacg gcgtcgctac
    23041 cgaaatgctc gatgaaggtc tggttcgtga agatctgacc gcgctggatt ttgtcaccat
    23101 cgacagtgcc agcacagaag atatggatga cgcccttttc gctaaggcgt tgccggatga
    23161 caaacttcag ctgattgtgg cgattgccga tccaaccgcg tggattgctg aaggcagcaa
    23221 gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat ctgcctggct tcaacatccc
    23281 tatgcttcct cgcgagcttt ctgacgatct ctgctcactg cgcgccaatg aagtccgccc
    23341 ggtactggca tgccgcatga cgctctccgc tgatggcacc attgaagata atatcgaatt
    23401 ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat gaccaggttt ctgactggct
    23461 ggaaaatacc ggtgactggc agcctgaaag tgaagcaatt gccgaacaag tccgtttgct
    23521 agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac cacgcactgg tgtttaaaga
    23581 tcgcccggat taccgcttta ttctcggtga aaaaggtgaa gtgctggata tcgtcgccga
    23641 gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg attgccgcta acatttgtgc
    23701 ggcccgcgtt ctgcgcgata agctcggttt tggcatctat aacgtgcata tgggctttga
    23761 tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg cacggtctgc atgtcgatgc
    23821 cgaagaagtg ctcacgctgg acggtttctg caaactgcgt cgtgaactgg atgcgcaacc
    23881 aactggtttc ctcgacagcc gcattcgtcg cttccagtca tttgctgaaa ttagcactga
    23941 acccggtcct cactttggcc tcggtctgga agcatacgcc acctggactt cgccgatccg
    24001 taaatatggc gacatgatca accaccgtct gctgaaagcg gttatcaaag gcgaaactgc
    24061 gacgcgtccg caggatgaaa tcactgtcca aatggccgaa cgtcgccgtc tcaaccggat
    24121 ggcagaacgt gatgttggcg actggttata cgcacgcttc ctgaaagata aagccgggac
    24181 cggcacccgt ttcgcggcgg aaattgtcga tatcagccgt ggcggcatgc gtgtgcgttt
    24241 ggttgataac ggcgctatcg cctttattcc ggcacctttc ttacacgctg tgcgcgatga
    24301 actggtttgc agccaggaaa acggcactgt acaaattaaa ggtgaaacgg tttacaaagt
    24361 aactgacgtt attgacgtca ccattgccga agtccgcatg gaaacccgcg gcattattgc
    24421 gcgcccggtc gcgtaatctc ctttcacggc ccattcctca tggatgggcc gtttatttcc
    24481 ccgctctacc ttcgttatat ccaggcagat tttttaactg actttcgttt gaaaactggc
    24541 gtttttccaa aactggatta caaataaata tataaaaaaa caatgcgttt agccgccgtt
    24601 aatttcatgt aagtcatgtt gttcctttta tttaaccgcg gactccgcag ttaaccggag
    24661 gatatgcatc atgaaaaccg ttagggagtc cacaacgttg tacaactttc tcggatcgca
    24721 caatccatac tggcggttga cggaaagcag cgatgttttg cgctttttta ccaccgaaac
    24781 cacagaacct gatcgtactt tgcagttatc tgccgaacag gctgctcgca tcagggaaat
    24841 gacggtcatc acctccagcc tgatgatgag tctgaccgtc gatgagagcg atctttctgt
    24901 gcatctggta ggacgaaaaa tcaataaacg ggaatgggct ggcaacgcgt ctgcatggca
    24961 tgacacaccg gcagttgctc gtgatttatc acacgggctt tcctttgctg agcaggtagt
    25021 ttctgaagca cattccgcaa tagtgattct cgacagccgg gggaatatcc aacgcttcaa
    25081 tcggttatgt gaagattaca ctgggttgaa agaacacgac gtcattggcc aaagcgtgtt
    25141 taaactgttt atgagccgtc gtgaagctgc ggcatccagg cgcaataacc gtgtattttt
    25201 tcgaagcggc aatgcatatg aagtcgaact gtggatacca acacgtaaag gccagcggct
    25261 gtttctgttt cgcaataaaa taaatttgtc cacagcggca gtggcaaaaa cgagattttt
    25321 ttaatctgtt ccggtaccga cattaccgaa gagcgccgcg ctcaggagcg actgcgtatt
    25381 ctggcaaata ccgacagtat caccggactg ccgaatcgta acgcaatgca ggagttaatc
    25441 gatcacgcta ttaatcaggc tgataacaat aaagttgggg ttgtgtatct tgatttggat
    25501 aattttaaaa aggtcaacga cgcctatggg catttgtttg gtgaccagtt attacgcgac
    25561 gtgtcattgg ctattttaag ctgtctcgaa catgaccagg tgttggcgcg tccaggtggc
    25621 gatgagtttc tggtactggc atccaatacc tcacaaagcg cgctggaagc aatggcatca
    25681 cgaattttga cccgcttacg cctccccttt cgtattggtt taattgaagt ttataccagc
    25741 tgttcagtag gtatttcgct ctctcccgaa catggttcgg acagcgcgac tattattcgt
    25801 cacgccgaca cagcaatgta cacagcgaag gaaggcggac gaggacaatt ttgcgttttt
    25861 actccagaaa tgaatcagcg ggtatttgaa tatctctggc tggataccaa cttgcgtaaa
    25921 gcactggaaa acgatcagtt ggttattcac tatcaaccga aaatcacctg gcgtggcgaa
    25981 gtgcgcagtc tggaagcact agtacgttgg cagtcacctg aacgtgggtt gattccaccg
    26041 ttggacttca tttcctacgc cgaagagtca gggctaattg tgcctttagg ccgttgggtg
    26101 attctcgatg tcgtacgcca ggtggcaaag tggcgggata aaggcataaa cctgcgagtg
    26161 gcggtaaata tttctgcacg tcagctcgcc gatcaaacca ttttcaccgc cctgaaacag
    26221 gttctccagg aactcaattt tgaatactgc cctatagatg ttgaactgac agagagttgt
    26281 ctgattgaga atgatgaact ggcactgtct gttattcaac aatttagcca actaggtgcg
    26341 caagtgcatc tggatgattt tggtaccggc tactcttcac tttcgcaact ggcgcgcttt
    26401 ccgatcgatg ccatcaaact tgaccaggtt tttgttcgag atattcacaa acaacctgtc
    26461 tcgcagtcac tggtccgggc gatcgtcgct gtggcccagg cattgaatct tcaggtgatc
    26521 gccgaaggtg tagagagtgc aaaggaagat gcttttttaa ccaagaacgg gatcaatgag
    26581 cggcaaggat ttttgtttgc caaaccgatg cccgccgtcg ccttcgaacg ctggtataaa
    26641 cgctatctga agcgcgcata agtagatgaa tatatatact tagcgctata tcccgtcatt
    26701 atatatcgta ttcctggatg ataattagcc gttgctgtta aacacttctc ttttagcggc
    26761 aatttttgca ttattcattt tccacttttg ctataaggat gtccttgatg tctgaatttg
    26821 atgcccagcg cgttgccgaa cgtattgata ttgtgttaga tatccttgtg gcaggcgatt
    26881 accactctgc gatccataat ctcgagatac ttaaagcaga actcttgcgc caggttgctg
    26941 aatctacgcc agacatcccg aaggcaccgt gggagattta acaatcgtgc cttcaggttt
    27001 tcctatttga ggatgcccgg c
//
LOCUS       DAZOYL010000021        26060 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZOYL010000021 DAZOYL010000000
VERSION     DAZOYL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26060
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.021"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1170
                     /gene="proV"
                     /locus_tag="ACHUOY_000647"
     CDS             <1..1170
                     /gene="proV"
                     /locus_tag="ACHUOY_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR0681517.1"
                     /translation="KIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGE
                     IFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLREVRRKKIAMVFQ
                     SFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQ
                     RVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAM
                     RIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGL
                     IRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALID
                     APLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1163..2227
                     /gene="proW"
                     /locus_tag="ACHUOY_000648"
     CDS             1163..2227
                     /gene="proW"
                     /locus_tag="ACHUOY_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR0681518.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2285..3277
                     /gene="proX"
                     /locus_tag="ACHUOY_000649"
     CDS             2285..3277
                     /gene="proX"
                     /locus_tag="ACHUOY_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR0681519.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3568..4752
                     /locus_tag="ACHUOY_000650"
     CDS             3568..4752
                     /locus_tag="ACHUOY_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0681520.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4876..5613
                     /gene="ygaZ"
                     /locus_tag="ACHUOY_000651"
     CDS             4876..5613
                     /gene="ygaZ"
                     /locus_tag="ACHUOY_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR0681521.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5603..5938
                     /gene="ygaH"
                     /locus_tag="ACHUOY_000652"
     CDS             5603..5938
                     /gene="ygaH"
                     /locus_tag="ACHUOY_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR0681522.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6029..6559
                     /gene="emrR"
                     /locus_tag="ACHUOY_000653"
     CDS             6029..6559
                     /gene="emrR"
                     /locus_tag="ACHUOY_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR0681523.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6686..7858
                     /gene="emrA"
                     /locus_tag="ACHUOY_000654"
     CDS             6686..7858
                     /gene="emrA"
                     /locus_tag="ACHUOY_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR0681524.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7875..9413
                     /gene="emrB"
                     /locus_tag="ACHUOY_000655"
     CDS             7875..9413
                     /gene="emrB"
                     /locus_tag="ACHUOY_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR0681525.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9477..9992)
                     /gene="luxS"
                     /locus_tag="ACHUOY_000656"
     CDS             complement(9477..9992)
                     /gene="luxS"
                     /locus_tag="ACHUOY_000656"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR0681526.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10142..11698)
                     /gene="gshA"
                     /locus_tag="ACHUOY_000657"
     CDS             complement(10142..11698)
                     /gene="gshA"
                     /locus_tag="ACHUOY_000657"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR0681527.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(11771..12199)
                     /locus_tag="ACHUOY_000658"
     CDS             complement(11771..12199)
                     /locus_tag="ACHUOY_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR0681528.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12196..12762)
                     /gene="yqaB"
                     /locus_tag="ACHUOY_000659"
     CDS             complement(12196..12762)
                     /gene="yqaB"
                     /locus_tag="ACHUOY_000659"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR0681529.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13043..13119)
                     /locus_tag="ACHUOY_000660"
     tRNA            complement(13043..13119)
                     /locus_tag="ACHUOY_000660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13083..13085),aa:Arg,seq:acg)
     gene            complement(13318..13394)
                     /locus_tag="ACHUOY_000661"
     tRNA            complement(13318..13394)
                     /locus_tag="ACHUOY_000661"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13358..13360),aa:Arg,seq:acg)
     gene            complement(13458..13534)
                     /locus_tag="ACHUOY_000662"
     tRNA            complement(13458..13534)
                     /locus_tag="ACHUOY_000662"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13498..13500),aa:Arg,seq:acg)
     gene            complement(13538..13630)
                     /locus_tag="ACHUOY_000663"
     tRNA            complement(13538..13630)
                     /locus_tag="ACHUOY_000663"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13594..13596),aa:Ser,seq:gct)
     gene            complement(13946..14131)
                     /gene="csrA"
                     /locus_tag="ACHUOY_000664"
     CDS             complement(13946..14131)
                     /gene="csrA"
                     /locus_tag="ACHUOY_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR0681530.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14366..16996)
                     /gene="alaS"
                     /locus_tag="ACHUOY_000665"
     CDS             complement(14366..16996)
                     /gene="alaS"
                     /locus_tag="ACHUOY_000665"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR0681531.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17124..17624)
                     /gene="recX"
                     /locus_tag="ACHUOY_000666"
     CDS             complement(17124..17624)
                     /gene="recX"
                     /locus_tag="ACHUOY_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR0681532.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            complement(17852..18913)
                     /gene="recA"
                     /locus_tag="ACHUOY_000667"
     CDS             complement(17852..18913)
                     /gene="recA"
                     /locus_tag="ACHUOY_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0681533.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(18993..19490)
                     /gene="pncC"
                     /locus_tag="ACHUOY_000668"
     CDS             complement(18993..19490)
                     /gene="pncC"
                     /locus_tag="ACHUOY_000668"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR0681534.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19635..20720)
                     /gene="mltB"
                     /locus_tag="ACHUOY_000669"
     CDS             complement(19635..20720)
                     /gene="mltB"
                     /locus_tag="ACHUOY_000669"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR0681535.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            20976..21539
                     /gene="srlA"
                     /locus_tag="ACHUOY_000670"
                     /gene_synonym="gutA"
     CDS             20976..21539
                     /gene="srlA"
                     /locus_tag="ACHUOY_000670"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR0681536.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21536..22495
                     /gene="srlE"
                     /locus_tag="ACHUOY_000671"
                     /gene_synonym="gutE"
     CDS             21536..22495
                     /gene="srlE"
                     /locus_tag="ACHUOY_000671"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR0681537.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            22506..22877
                     /gene="srlB"
                     /locus_tag="ACHUOY_000672"
                     /gene_synonym="gutB"
     CDS             22506..22877
                     /gene="srlB"
                     /locus_tag="ACHUOY_000672"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR0681538.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            22881..23649
                     /gene="srlD"
                     /locus_tag="ACHUOY_000673"
                     /pseudo
     CDS             22881..23649
                     /gene="srlD"
                     /locus_tag="ACHUOY_000673"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            23755..24114
                     /gene="gutM"
                     /locus_tag="ACHUOY_000674"
     CDS             23755..24114
                     /gene="gutM"
                     /locus_tag="ACHUOY_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR0681539.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            24181..24954
                     /gene="srlR"
                     /locus_tag="ACHUOY_000675"
                     /gene_synonym="gutR"
     CDS             24181..24954
                     /gene="srlR"
                     /locus_tag="ACHUOY_000675"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR0681540.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            24947..25912
                     /gene="gutQ"
                     /locus_tag="ACHUOY_000676"
     CDS             24947..25912
                     /gene="gutQ"
                     /locus_tag="ACHUOY_000676"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR0681541.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(25909..>26060)
                     /locus_tag="ACHUOY_000677"
     CDS             complement(25909..>26060)
                     /locus_tag="ACHUOY_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681542.1"
                     /translation="LREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKR
                     LGMKD"
BASE COUNT         6039 a         6975 c         6635 g         6411 t
ORIGIN      
        1 aaaatatttg gtgagcatcc acagcgagca ttcaaatata tcgaacaagg actttcaaaa
       61 gaacaaattc tggaaaaaac tgggctatcg cttggcgtaa aagacgccag tctggccatt
      121 gaagaaggcg agatatttgt catcatggga ttatccggct cgggtaaatc cacaatggta
      181 cgccttctca atcgcctgat tgaacccacc cgcgggcaag tgctgattga tggtgtggat
      241 attgccaaaa tatctgatgc caaactccgt gaggtgcgca gaaaaaagat tgcgatggtc
      301 ttccagtcct ttgccttaat gccgcatatg accgtgctgg acaatactgc gttcggtatg
      361 gaattggccg gaattaatgc cgaagaacgc cgggaaaaag cccttgatgc actgcgtcag
      421 gtcgggctgg aaaattatgc ccacagctac ccggatgaac tctctggcgg gatgcgtcaa
      481 cgtgtgggat tagcccgcgc gttagcgatt aatccggata ttttattaat ggacgaagcc
      541 ttctcggcgc tcgatccatt aattcgcacc gagatgcagg atgagctggt aaaattacag
      601 gcgaaacatc agcgcaccat tgtctttatt tcccacgatc tcgatgaagc catgcgtatt
      661 ggcgaccgaa ttgccattat gcaaaatggt gaagtggtac aggtcggcac accggatgaa
      721 attctcaata atccggcgaa tgattatgtc cgtaccttct tccgtggcgt tgatattagt
      781 caggtattca gtgcgaaaga tattgcccgc cggacaccga atggcttaat tcgtaaaacc
      841 cctggcttcg gcccacgttc ggcactgaaa ttattgcagg atgaagatcg cgaatatggc
      901 tacgttatcg aacgcggtaa taagtttgtc ggcgcagtct ccatcgattc gcttaaaacc
      961 gcgttaacgc agcagcaagg tcttgatgcg gcgctgattg atgcgccgtt agcagtcgat
     1021 gcacaaacgc ctcttagcga gttgctctct catgtcggac aggcaccctg tgcggtgccc
     1081 gtggtcgacg aggaccaaca gtatgtcggc atcatttcga aaggaatgct gctgcgcgct
     1141 ttagatcgtg agggggtaaa taatggctga tcaaaataat ccgtgggata ccacgccagc
     1201 ggcggacagt gccgcgcaat ccgcagacgc ctggggtaca ccgacgactg caccgactga
     1261 cggcggtggt gctgactggc tgaccagtac gcctgcgcca aacgtcgagc attttaatat
     1321 tctcgatccg ttccataaaa cgctgatccc gctcgacagt tgggtcactg aagggatcga
     1381 ctgggttgtt acccatttcc gtcccgtctt ccagggcgtg cgcgttccgg ttgattatat
     1441 cctcaacagt ttccagcaat tgctgctggg tatgccagcg ccagtggcga ttatcgtttt
     1501 cgctctcatc gcctggcaga tttccggggt cggaatgggc gtggcgacgc tggtttcgct
     1561 gattgccatc ggcgcaatcg gtgcctggtc gcaggcaatg gtgactctgg cgctggtgtt
     1621 aaccgccctg ctgttctgta tcgtcatcgg tttgccgttg gggatatggc tggcgagaag
     1681 tccgcgagcg gcgaaaatta ttcgtccact gcttgatgcc atgcagacca cgccagcgtt
     1741 tgtttatctg gtaccaatcg tcatgctgtt tggtatcggt aacgtgccgg gcgtagtggt
     1801 gacgatcatc tttgctctgc cgccgattat ccgtctgacg attctgggga ttaaccaggt
     1861 tccggcggat ctgattgaag cctcgcgctc attcggtgcc agcccgcgcc agatgctgtt
     1921 caaagttcag ttaccgctgg cgatgccgac cattatggcg ggcgttaacc agacgctgat
     1981 gctggctctt tctatggtgg tcatcgcctc gatgattgcc gtcggcgggc tgggtcagat
     2041 ggtacttcgc ggtatcggtc gtctggatat gggccttgcc accgttggcg gcgtcgggat
     2101 tgtgatcctc gccattatcc tcgaccgcct gacgcaggcc gttgggcgcg actcacgcag
     2161 tcgcggcaac cgtcgctggt acaccactgg ccctgtcggt ctgctgaccc gcccattcat
     2221 taagtaactc tgcacttgcc cggtaacgcc gggcattatc accctgccaa aaaaaggaat
     2281 aacaatgcga catagcgtac tttttgcgac agcgtttgcc acgcttatct ctacacaaac
     2341 ttttgcagcc gatctgccgg gcaaaggcat tactgttaat ccagttcaga gcaccatcac
     2401 tgaagaaacc ttccagacgc tgctggtcag tcgtgcactg gagaaattag gttataccgt
     2461 caataaaccc agcgaagtgg attacaacgt tggctacacc tcacttgctt ccggcgatgc
     2521 aaccttcacc gccgtgaact ggacgccgct gcatgacaac atgtacgaag ctgccggtgg
     2581 cgataagaaa ttttattgtg aagggatatt cgttaacggc gcggcacagg gttacctgat
     2641 cgataagaaa accgccgacc agtacaaaat caccaacatc gcacaactga aagatccgaa
     2701 gatcgccaaa ctgttcgata ccaacggcga cggtaaagcg gatttaaccg gctgtaaccc
     2761 aggctggggc tgcgaaggtg cgatcaacca ccagcttgcc gcgtatgaac tgaccaacac
     2821 cgtgacgcat aatcagggga actacgcagc gatgatggcc gacaccatca gtcgctacaa
     2881 agagggcaaa ccggtgtttt attacacctg gacgccgtac tgggtgagta atgagctgaa
     2941 gccagggaaa gatgtggtct ggttgcaggt gccgttctcc gcactgccgg gcgataaaaa
     3001 cgccgatacc aaactgccga atggtgcgaa ttatggcttc ccggtcagca ccatgcatat
     3061 cgttgccaac aaagcctggg ccgagaaaaa cccggcagca gcgaaacttt ttgccattat
     3121 gcagttgcca gtggcagata ttaacgccca gaacgccatt atgcatgacg gcaaagcctc
     3181 agaaggcgat attcagggcc atgttgatgg ttggatcaaa gcccaccagc agcagttcga
     3241 tggctgggtg aatgaggcgc tggcagcgca gaagtaattt ttattcgggc ggataagtcg
     3301 tttttgcctc atccgccatt ctgtgcacaa tgcctgatgc gacgctggcg cgtcttatca
     3361 tgcctacaaa atacactcat tccgtatggc ggataaggcg ttttcgccgc atccgccgtt
     3421 ctgtgcacaa tgcctgatgc gacgctggcg cgtcttatca tgcctacaaa ccacatcgca
     3481 catttcaaca aactatcttt cgcccccatt catcaacatc cgctattatt gatttccagc
     3541 ttaatcatca cctgatgaac aaaaataatg actaaaccta atcatgagct tagcccggcg
     3601 ctgatcgtgc tgatgtctat cgccaccggt caggcggtag ccagtaacta ttacgcccag
     3661 ccattgctcg acaccatcgc gcgtaacttt tccctttccg ccagttcggc aggctttatt
     3721 gttaccgccg cgcagttggg ctatgcagca ggtctgctgt ttcttgttcc cctcggtgat
     3781 atgtttgaac gccgccgcct gattgtctcg atgaccttac tggcggcagg cggcatgtta
     3841 atcactgcca gcagtcagtc gctggcgatg atgatcctcg gtacggcatt aaccggttta
     3901 ttctcagtcg tggcacaaat tctggttccg ctggcagcga cgctggcttc accggataaa
     3961 cgcggcaaag tggttggcac cattatgagc ggtctgctgt tggggatctt gctggcacga
     4021 accgttgccg ggttactggc aaacctcggc ggctggcgca ccgtcttttg ggttgcctcg
     4081 gtgttaatgg cactgatggc gctagcatta tggcgtggtc tgccacaaat gaaatcagaa
     4141 acccacctca actacccaca gttgttaggt tccgttttca gcatgtttat cagcgataag
     4201 atcctccgca cccgcgcatt gctgggctgc ctgacctttg ccaacttcag cattctctgg
     4261 acctcaatgg cctttttgct tgccgctcca ccttttaact acagcgatgg tgtcattggt
     4321 ctgtttggac ttgcgggagc tgccggagcg ttgggcgctc gtccggcggg cggttttgcc
     4381 gataagggta aatcgcacca caccacaact ttcggtctgc tgctgctatt actttcatgg
     4441 ctggcgatct ggtttggtca cacttccgta ctggcgctga ttatcggcat cctggtgctg
     4501 gatctcaccg tacagggcgt gcatatcact aaccagacgg taatttatcg aatacaccct
     4561 gatgcgcgta atcgcctgac cgcaggttac atgaccagct actttattgg cggtgccgcc
     4621 ggttcgctaa tttcagcctc agcctggcaa catggcggtt gggctggcgt ttgtctggct
     4681 ggcgcgacga ttgccctagt taacttactg gtctggtggc gaggttttca tcgtcaggaa
     4741 gccgcaaatt aagcaatcag caaccgtttt cgtggcttta cacttataag ggtgttaaga
     4801 agcccatcag tctgataagg ttaagatatt cattcagtct atttataata ttaacaatcg
     4861 ttaagcgtac actctatgga aagccctact ccacagcctg ctcctggttc ggcgaccttc
     4921 atggaaggat gcaaagacag tttaccgatt gttattagtt atattccggt ggcctttgcg
     4981 ttcggtctga atgcgacccg tctgggattc tctcctctcg aaagcgtttt tttctcctgc
     5041 atcatttatg caggcgcgag ccagttcgtc attaccgcga tgctggcagc cgggagtagt
     5101 ttgtgggttg ctgcactgac cgtcatggca atggatgttc gccatgtgtt gtatggcccg
     5161 tcactgcgta gccgtattat tcagcgtctg caaaaatcga aaaccgccct gtgggcgttt
     5221 ggcctgacgg atgaggtttt tgccgccgca acagcaaaac tggtacgcaa taatcgccgc
     5281 tggagcgaga actggatgat cggcattgcc ttcagttcat ggtcatcgtg ggtatttggt
     5341 acggtaatag gggcattctc tggcagcggc ttgctgcaag gttatcccgc cgttgaagca
     5401 gcattaggtt ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc tttccagcgc
     5461 aaacaatctc tttgcgttac cgcagcgtta gttggtgccc ttgcaggcgt aacgctattt
     5521 tctattcccg tcgccattct ggcaggcatt gtctgtggct gcctcactgc gttaatccaa
     5581 gcattctggc aaggagcgcc cgatgagcta tgaggttctg ctgcctgggt tactggttgg
     5641 cgcggcgaat tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta atgcccgccc
     5701 aaccaaacgt ggcgcggtag gtattttgct cgacaccatt ggcatcgcct cgatatgcgc
     5761 tctgctggtt gtctctaccg caccagaagt gatgcacgat acacgccgtt tcgtgcccac
     5821 gctggtcggc ttcgcggtac tgggtgccag tttctataaa acacgcagca ttatcatccc
     5881 aacactgctt agtgcgctgg cctatgggct cgcctggaaa gtgatggcga ttatataacc
     5941 cacaagaatc atttttctaa aacaatacat ttactttatt tgtcactgtc gttactatat
     6001 cggctgaaat taatgaggtc atacccaaat ggatagttcg tttacgccca ttgaacaaat
     6061 gctaaaattt cgcgccagcc gccacgaaga ttttccttat caggagatcc ttctgactcg
     6121 tctttgcatg cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc tgaaggctca
     6181 ggggattaac gagacgttgt ttatggcgtt gattacgctg gagtctcagg aaaaccacag
     6241 tattcagcct tctgaattaa gttgtgctct tggatcatcc cgtaccaacg cgacgcgtat
     6301 tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt gaaagcgata acgatcgccg
     6361 ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg ttttaccacc
     6421 gcagcataac tgcctgcatc aactctggtc cgcgctcagc acaacagaaa aagatcagct
     6481 cgagcaaatc acccgcaaat tgctctcccg tctcgaccag atggaacaag acggtgtggt
     6541 tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat ccagatttat aaaagaaaaa
     6601 atgaccggcc agcatcgcaa catgctggcc tttttggcaa gcaggtcggc tcagccgatg
     6661 agttaagaag atcgtggaga acaatatgag cgcaaatgcg gagactcaaa ccccgcagca
     6721 accggtaaag aagagcggca aacgtaagcg tctgctcctc cttctcacct tgctctttat
     6781 aattattgcc gtagcgatag ggatttattg gtttttggta ctgcgtcatt tcgaagaaac
     6841 cgatgacgca tacgtggcag ggaatcagat tcaaattatg tctcaggtgt ctggcagcgt
     6901 gacgaaagtc tgggccgata acaccgattt tgtaaaagaa ggcgacgtgc tggtcactct
     6961 cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa actgcactgg cttccagcgt
     7021 tcgccaaacc caccagctga tgattaacag caaacagttg caggcgaata ttgaggtgca
     7081 gaaaatcgcc ctcgcaaaag cacaaagcga ctacaaccgc cgtgtgccgc tgggcaatgc
     7141 caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca gtgcccaggc
     7201 gcaactggac gtcgctattc aacaatacaa tgccaatcag gcgatgattc tggggactaa
     7261 actggaagat cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg cctggctggc
     7321 gctggagcgt acccgtattg tcagtccgat gaccggttat gtctcccgcc gtgcggtaca
     7381 gcctggggcg caaattagcc caacgacgcc gctgatggcg gtcgttccag ccaccaatat
     7441 gtgggtggat gccaacttta aagagacgca gattgccaat atgcgtatcg gtcagccggt
     7501 cactatcacc acggatattt acggcgatga tgtgaaatac accggtaaag tggttggtct
     7561 ggatatgggc acaggtagcg cattctcact gcttccggcg caaaatgcga ccggtaactg
     7621 gatcaaagtc gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac agctggagca
     7681 atacccgctg cgcattggtt tgtccacact ggtgagcgtc aataccacta accgtgacgg
     7741 tcaggtactg gcaaataaag tacgttcaac accggtcgcg gtaagtaccg cgcgtgaaat
     7801 cagcctggca cctgtcaata aactgatcga cgatatcgta aaagctaacg ctggctaatc
     7861 cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg gtcattatga
     7921 cgattgcgct gtcactggcg acattcatgc aggtgctgga ctccaccatt gctaacgtgg
     7981 cgatccccac tatcgccggg aatctgggct catcgctcag ccagggaacg tgggtaatca
     8041 cttctttcgg ggtggcgaat gccatctcga tcccgcttac cggctggctg gcaaagcgcg
     8101 tcggggaagt gaaactgttc ctttggtcca ccatcgcctt tgctattgcg tcgtgggcgt
     8161 gtggtgtctc cagcagcctg aatatgctga tcttcttccg cgtgattcag gggattgtcg
     8221 ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa taactacccg ccagccaaac
     8281 gctcgatcgc gctggcgttg tggtcgatga cggtgattgt cgcgccaatt tgcggcccga
     8341 tcctcggcgg ttatatcagc gataattacc actggggctg gatattcttc atcaacgtgc
     8401 cgattggcgt ggcggtggtg ttgatgacac tgcaaactct gcgcggacgt gaaacccgca
     8461 ccaaacggcg gcggattgat gccgtggggc tggcactgct ggttattggt atcggcagcc
     8521 tgcagattat gctcgaccgc ggtaaagagc tggactggtt ttcatcacag gaaattatca
     8581 tccttaccgt ggtggcggtg gtggctatct gcttcctgat tgtctgggag ctgaccgacg
     8641 ataacccgat agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc ggctgcttgt
     8701 gtatcagcct cgcgtatatg ctctacttcg gcgctattgt tctgctgccg cagttgttgc
     8761 aggaggtcta cggttacacg gcgacctggg caggtttggc ctctgcgccg gtagggatta
     8821 ttccggtgat cctgtcgccg attatcggcc gcttcgcgca taaactggat atgcggcggc
     8881 tggtaacctt cagctttatt atgtatgccg tctgcttcta ctggcgtgcc tatacctttg
     8941 aaccaggtat ggattttggc gcgtcggcct ggccgcagtt tatccagggg tttgcggtgg
     9001 cctgcttctt tatgccgctg accaccatta cgctgtctgg tttgccaccg gaacgactgg
     9061 cggcggcatc gagcctctct aactttacgc gaacgctggc ggggtctatc ggcacgtcga
     9121 taaccacgac catgtggacc aaccgcgagt cgatgcacca tgcgcagttg actgagtcgg
     9181 taaacccgtt caacccgaat gcccaggcga tgtacagtca actggaaggg cttgggatga
     9241 cgcaacagca ggcatcaggc tggattgccc agcagatcac caatcagggg ctgattattt
     9301 ccgccaatga gatcttctgg atgtcagccg ggatattcct cgtactgctg gggctggtgt
     9361 ggtttgctaa accgccattt ggcgcaggtg gcggtggcgg cggtgcgcac taagtacaac
     9421 taagccagtt catttgaact ggcttttttc aattaattgt gaagatagtt tactgactag
     9481 atgtgcagtt cctgcaactt ctctttcggc agtgccagtt cttcgttgct gttgatgcgt
     9541 acgtcacgtt ccagaatgct acgcgcaata tcctgcgctt cctgcaacga gtgcatctgg
     9601 taagtgccac actggtagac gttcagttcc gggatctgat tctgatcctg cactttcagc
     9661 acgtcttcca ttgccgcttt ccaggcatca gcaacacgct gctcatctgg cgtaccaatc
     9721 agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat ctctacacca
     9781 ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt atggatccct
     9841 ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac ggtgattgcg
     9901 tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc catccgggta
     9961 tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat ttttttaaaa
    10021 attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg catttgttat
    10081 catcatccct gaattcagag atgaaatttt ggccactcac gagtggcctt tttcttttct
    10141 gtcaggtgtg tttttccagc cacaccgcaa acggttcggt atcagcggtt tccatttcct
    10201 gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc agaatttcca
    10261 gcggctcttc acgcagcaga ttacggtagg cttctgcaaa cgctttgcct gttccgccaa
    10321 taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg ggattatcga
    10381 agcaggcaac cagttcatca cacactttct gatacgcgtc gccgccgtta atgctatcca
    10441 gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc agtaacggga
    10501 actgcgcggt ttcgcagccg atacccagcg tcaggcccgg tttgcgacct tcaaggatca
    10561 cccggttcca gttaacgcgc gtacaggcaa gttcgctact gctcatttcc ggtgcatcag
    10621 ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgctgttca tctacaccaa
    10681 tcggcgagaa cgggttgatg tccagcgaac gcacttcaat atattcaatg ccgccacgta
    10741 acagcgcatc agaaggtgac tcgccgctgc gggtaacgcg ttttggacga atcggcgcat
    10801 acagttcgtt ttcaatctgc agcacgttgc tattgatttg cagcctctta ccgtctttct
    10861 caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta aggcccgcta
    10921 cgtactcgta aagatcgttg aaggtaatac caagattgct ttgcgattta ttggtatagc
    10981 cgagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg cactcggttt
    11041 tctcaaacgg cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc gccggagatg
    11101 caccaaacag ataaggaatg acccaaccga aacgatagta attgcggata acgcggaaat
    11161 agcccgcaga aattttctct ttggcatcag cgcccgagat atcaccgcac ttcgcttgcc
    11221 agaatgccat tggcaaagag aaattgtagt gcacgccgga aatggtttgc atcagcgcgc
    11281 cgtagcgatt tttcagccct tcacgataca gcgttttaaa gcgtccggtg ttagaagtgc
    11341 cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta gcatggcata cttaacggcc
    11401 acatccgctc atcgcccata ttgcgcgccg tataacgatg cagatcgcgc ataaaggtca
    11461 gcatatgttc aatatcacca tccactggtg taatgaattc cagcaatgct tccgcaaaat
    11521 cggtagtaat ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga cctgttgttg
    11581 ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc tgtatcccct
    11641 ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc gggatcaaat
    11701 tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga ccatatgtgc
    11761 aggcctacaa ttagtgccac cacatcatgc cctgaacggt cgctgctgca actgcaacat
    11821 agcgtaacgc tttaccaagg cacaaaaaaa agattaccgg tccccacgag atgcgcatcc
    11881 atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat aatagcgtga
    11941 ctgcaccata gcgtttcagc cagccgatag ctttctcttg ccagcgcgat gttttacgca
    12001 atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta cccattgttg
    12061 ctgttaaaac taaaacccag ggatgactga tcccggaaag caacattgcc accagcacga
    12121 cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac aacgagaaaa
    12181 gcgataacgc ttcactcaca gcaagcgaac gtccacagcg tccatacctg cggcacgggc
    12241 cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt gcacgcccat
    12301 acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga cgtgatcggc
    12361 agcgacgacg gcgtcaaaat aatggcgtaa tcccaggtgc gccagcaatg cctcagcgat
    12421 ggcgctttca ctccccgttc ctacagccat tgggcgacga ccatgccaac ttttcaccac
    12481 atcaacaaga ggaagcggtt cgacgctatc cagcagcata cttcttactg cttctgtttt
    12541 ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa ttgcctgagc
    12601 aatacgccag gtgggcgatc cattaagcgc aatcatcgcc tgaatatcgt actgaagacc
    12661 gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggctccgtat ccaggattgt
    12721 gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct cgcaagtcga
    12781 aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggcg aaggagaaga
    12841 aaattcagga aatggataaa ggagttattt agaaatgaga tatttttgaa ggaaattttt
    12901 tggagaagat ggtgcatccg ggaggatgac tcactgacgt tcgcccttcg ggccgttgct
    12961 ggcgcaacgt tatccttcct ggtgcctgcg gttaataccg ctatctcttc gataccttca
    13021 ttgctgaagg ttacggagaa tatggtgcat ccgggaggat tcgaacctcc gaccgctcgg
    13081 ttcgtagccg agtactctat ccagctgagc tacggatgca tcgggaaaac ttattctact
    13141 gctgatattg ataccgctac taaagccata tcaattaaga gatggtgcat ccgggaggat
    13201 tactcgcctt gcggctcgcc cttcgggccg ttgctaaagc aacgttatca tccctggtgc
    13261 ctgcggttaa ttccgctacc tcttcgatac cttcattgct gaagatttcg gagaatatgg
    13321 tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc
    13381 tgagctacgg atgcatcggg aaaatttatt ttactgctga tattgatacc gctactaaag
    13441 ccatatcaag taagagatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    13501 agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg gggattcgaa
    13561 ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc agcctctcgg
    13621 ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc tcatcgtcgc
    13681 tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc ccacactttt
    13741 atcgtttgca cacttcacgc tcaattagtc tgtaaaaacg tcaaaaagag tgttttatct
    13801 acagaaaaac ggaggtctgg cagatagcag taatgcaaaa aaatggagac ttaagttgaa
    13861 tgaacgggag taaagcgaaa agactataga gtgaaggaga aattttgagg gtgcgtctca
    13921 ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt tcagcctgga
    13981 tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt acgccaatac
    14041 gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca atcatgaggg
    14101 tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat taaaagagtc
    14161 gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag caatgacaaa
    14221 cacattacat gtaagcagtc atggcattac attctgttaa acctaagttt agccgatata
    14281 cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc gtgaagcgag
    14341 tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag cctttcacac
    14401 tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc atgtcaggac
    14461 gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc cctgctttca
    14521 cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct tcggctaccg
    14581 ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc atggtacgca
    14641 acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca ttaacatcaa
    14701 ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta agctgttgta
    14761 gttctttttc taactgacgc gtacgttcca gtactgagcg cactttatcc gccagattat
    14821 tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct gcatgaacgg
    14881 tggtgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca gcagtacccg
    14941 attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga gtaccgccac
    15001 acagctcggt ggagaaatcg cccatgctca gcacgcgtac acgctcatca tacttctcgc
    15061 cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg ttggtttcga
    15121 tcggcaaatt gcgacgaatc tgtgcgttca ccaggtcttc gaccgcacga atctcttccg
    15181 gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac cttgtcgtta accagtgaac
    15241 ctttctgcga tacatgagtc cccagaacct ggcgcagcgc agcgtgcatc aggtgcgttg
    15301 cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc tgcacagcgt
    15361 cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc tggccgtatt
    15421 tctgcgtatc ttccaccgcg aaggagaagt tagcgccttt cagttcgcct ttatcaccaa
    15481 cctgaccgcc ggattccgca tagaatggcg tttgatccag cacgaccaca gcttcctggc
    15541 ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact ttgccgttca
    15601 gttccagatg gtcatagcct ttaaattcag atgcactatc aacacggatc attgcgttgt
    15661 aatcggcacc aaagccgctg gcttcgcgcg cgcgacgacg ctgctcttcc attgctgctt
    15721 caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc gtcaggtcaa
    15781 ccgggaagcc ataggtgtcg tacagacgga aagccgtttc accatccagc gtatcaccag
    15841 aaagttttgc cagctcttca tccagcaacg ccagaccgcg ctccagagta cgggcaaact
    15901 gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc aggtcttcac
    15961 ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag gtttctttcg
    16021 cgccgagcat attgccgtga cgcactgcgc gacgaatgat acgacgcagt acataaccac
    16081 ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa cgaatgtggt
    16141 cagcgattac gcgcagcgat ttattgctca gatcggtcgc gccagtgact ttcgctaccg
    16201 cctggatcaa cgtgcggaac aggtcgatgt catagttaga gttaacgtgt tgcagcaccg
    16261 cagcaatacg ctccagaccc ataccggtat ctacagacgg cttcggcagc ggttccatcg
    16321 tgccatctgc ctggcggttg aactgcatga agacgatgtt ccagatctca atgtagcggt
    16381 cgccgtcttc ttccggactt cccggagggc caccccaaat gtggtcgccg tgatcgtaga
    16441 agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag ttgtcagatg
    16501 cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg atccctactt
    16561 ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc cacagacgct
    16621 ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac tgaatggcat
    16681 cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag gtatggtgac
    16741 gcgcggtgta accgacattt tccaggtcgt tgtgtttacc acccgcacgc acgcagcgtt
    16801 gggaagtggt agcgcgggaa taattacgct tgtcgagtcc aaggaacaca tccttgaact
    16861 ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc agggagctgc
    16921 tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga cggatctcag
    16981 cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgcc accagctcca
    17041 gcgtgtctta accgccgggc tggtaactga aaagtgggaa taagataagt tttcttgagt
    17101 gggaagtaaa ataccgtatg cgttcagtcg gcaaaattta gccaaatatc ctggatatct
    17161 tccatcagat agccacgata gagcagaaaa cgctggatct taactttttc tgaaaagaca
    17221 gttggcaaag gttcaccata ttttcgcgtc gcctgatcgc gcaccagtgc gcaccagtcg
    17281 atgtcacatt cacgcatcgc tttttctgtc gcttcgcggg aaataccttt ctgattcagt
    17341 tcctgacgaa tacgtgcagg tccataacct ttgcggctac ggctggcgat aaagcgcgca
    17401 acaaatcggc tgtcatcgag atagccatgt tcatggcacc aggcaataac gcgctcgtaa
    17461 tcttctgccg tagcatcaat ctcttctggg ccatttttgc ccataatcgg tgccgcgagt
    17521 ttacgtcgca gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac cgcacgatcc
    17581 aacaggcgag catatgccgg gcgacgggat gttgattctg tcatggcata tccttacaac
    17641 ttaaaaaagc aaaagggccg cagatgcgac ccaggttttg ctgataaaca gatttaatag
    17701 tcaatgagtg tgcagccaat tcgttagagg atcgaggtga gagtaatttt tcacgccagt
    17761 gttggcctgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgtccgtgca
    17821 cacattatct attatcatca ataacgaacg attaaaaatc ttcgttagtt tctgctacgc
    17881 cttcgctgtc atctacagag aaatccggcg ttgagttcgg gttgctcagc agcaactcac
    17941 gtactttctt ctcgatctct ttcgcggttt ccgggttatc tttcagccag gcagttgcat
    18001 tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct gctttctcga
    18061 tcagcttctc ttttacgccc aggtcaacca gttcgccgta gaagttgata ccttcgccgt
    18121 agaggatctg aaattcagcc tgtttaaacg gtgcagcgat tttgttcttc accactttca
    18181 cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata cgacggatgt
    18241 cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtggtt tccgggttac
    18301 cgaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc gtgttggact
    18361 gcttcaggtt acccgccagc ttacgcatcg cctggctcat catacgtgcc gcaaggccca
    18421 tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc gccacggagt
    18481 caacgacgat aacgtctact gcgccagaac gcgccagggc gtcacagatt tccagtgcct
    18541 gctcgccggt gtccggctgg gagcacagca ggttatcgat atcgacgccc agtttacgtg
    18601 cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta ccttcacgct
    18661 gcgctgcggc gatcacctgc agcgtcagcg tggttttacc ggaagattcc ggtccgtaga
    18721 tttcgacgat acggcccatc ggcagaccac ctgccccaag cgcgatatcc agtgaaagcg
    18781 aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc atgatggagc
    18841 ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc tgtttgtttt
    18901 cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca atatgttctg
    18961 ttgaagcaat tatactgtat actcatacag tatcaagtgt tttgtagaaa ttgttgccac
    19021 aaggtctgca atgcatacgc agtagcctga cgacgcaccg catcacggtc gccgctgaag
    19081 cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca gacggtgccg
    19141 acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga cacggcataa
    19201 tcagcacgag ccgctttcag tgcacctatc gccatttcca ccacgacggg ttcactcacc
    19261 gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt ggcttcgtta
    19321 ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc ggtaatcact
    19381 ttcgctaccc aaccaccggt acaagactcg gcagttgtta cggttgcgcc acgggctttc
    19441 agcgcctgcc caacctgttc gcttaactgc atcagttcac tgtcagtcat cacaacctca
    19501 gtcagtcaaa aatttatgcc ggacaagata gcactttcgt caggaagatg gtgttggcgt
    19561 tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag ctcacgcccc
    19621 cctcttgcaa atagttactg cacgcgcgcc agcgccacgg cttgtcctaa ctgccagacc
    19681 gccattgcgt aatgggtgct gtggttataa cgggtaatgg tgtagaagtt cggcagaccg
    19741 taccagtact ggtaaccggt accgacatcc agacgcagca ggctggcttg ttgatggttg
    19801 cccagcggct gctgtggcgt taaacctgcc gtggcaagct gcgaaatgct gtacctggtt
    19861 ttgaagccat ttggcaagcc tggagcctga ccgtttgcca ttaccgcgac ctgatcgcct
    19921 ttcacccagc cgtgcgcttt gaaatagttc gccacactac cgatcgcatc aaccggatcc
    19981 cacaggttga tatgcccgtc gccgctgaaa tctaccgcat attgtttgta agacgacggc
    20041 ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag cggatcgtcc
    20101 tgctcgtcgc gcgccatcaa caggaaggtt tccagctcgc cagagaaata ctccgcgcgg
    20161 cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt ccccatcacg
    20221 cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg tacgccatac
    20281 acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac accgttctgc
    20341 acgttgtccg gcgtaataaa ttttttgcga taacgcagcc atgcgccgtt cggacctgat
    20401 ggcggtttca ccgatgtggt tggtgcctgg ttatccatca gccgcagtac cgaatccaga
    20461 cgcttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg tttgttcacc
    20521 attctgtcga tgaactgctg ggcattcggg ttattagcga aatcgccgcc catctgcatc
    20581 acattgtgct gaggctcaag caggaagccg ccagacggcg ttccggtggt cgtttcagtc
    20641 tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg aagcaatgtt
    20701 acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg gcaagtatgg
    20761 taaagcatca cgccccgcac aaggaagcgg tagtcactgc ccgatacgga ctttacataa
    20821 ctcaactcat tcccctcgct atccttttat tcaaactttc aaattaaaat atttatcttt
    20881 cattttgcga tcaaaataac actttcaaat ctttcaatct gattagatta ggttgccgtt
    20941 tggtaataaa acaataaatc ctgaaggaga gaacaatgat agaaaccatt actcatggtg
    21001 cagagtggtt tatcgggctg ttccaaaagg gcggagaggt gtttaccggg atggtgaccg
    21061 gcattcttcc gctgttgatt agcctgctgg ttatcatgaa cgcactgatt aattttatcg
    21121 gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg taaccctgtt tcccgttacc
    21181 tgctgctacc gtgcattggc acgtttgtct tttgcaatcc gatgacctta agcctgggtc
    21241 gctttatgcc ggaaaagtac aaacccagct actacgcggc agcctcttat agctgccact
    21301 caatgaatgg cctctttccc catatcaacc ctggcgaact gtttgtttat cttggcattg
    21361 ccagcggtct gacaacgctg aacctgccac ttggcccact ggcggtgagc tatctgctgg
    21421 ttggtctggt caccaacttc tttcgcggct gggtgaccga tctgaccacc gccatttttg
    21481 agaaaaagat gggcattcaa cttgaacaaa aagttcacct ggcaggagca acatcatgac
    21541 gcgtattcgg atcgaaaaag gaacgggtgg ctggggcggc ccgcttgagc tggaagccac
    21601 gccgggcaaa aaaatcgtct atatcaccgc cggtacccgg cctgcgattg ttgacaaact
    21661 ggcacaactt actggctggc aggctattga cggatttaaa gaaggtgaac ccgcggaggc
    21721 ggaaattggt gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca tctatccgaa
    21781 acgacgtatt cccaccatta atatccactc gacgggcaag tccagtccgc tggcgcagta
    21841 cattgtggaa gatatttatg tctctggcgt aaaagaagaa aacatcactg tagtgggtga
    21901 tgcgacacca caaccctctt ccgtgggccg tgactatgac accagcaaga aaatcaccga
    21961 acaaagcgat ggtttactgg cgaaggtggg aatgggtatg ggttctgccg ttgccgtgtt
    22021 gtttcaatct ggtcgtgaca ccatcgacac tgtattaaaa accattctgc cgtttatggc
    22081 gttcgtctcg gcgctcattg gcatcattat ggcttctggc cttggtgact ggattgccca
    22141 cggtcttgct ccgctggcga gccatccact aggtctggtc atgctggcgc tcatctgctc
    22201 cttcccgctg ctttcacctt tcctcggccc aggcgcagtt atcgcacagg ttatcggcgt
    22261 attgattggc gtgcagattg gtctcggcaa tattccgccg catctggctt taccggcact
    22321 gtttgccatc aacgcgcagg cggcctgcga cttcatcccg gtcggtttgt cgctggcgga
    22381 agcccgtcag gacacggttc gcgtcggtgt cccttctgta ctggtgagcc actttttaac
    22441 cggcgcaccg actgtactga tcgcctggtt tgtctccggt tttatctatc aatagaggct
    22501 gaaacatgac cgttatttat cagaccacca tcacccgtat cggcgcgagt gccactgacg
    22561 ccctcagcga ccagatgctc atcacctttc gtgaaggcgc gccagcggac ctcgaagagt
    22621 attgcttcat tcattgccac ggcgagttga aaggtgcact ccatcccggt ttgcaattta
    22681 cgctcgggca gcatcgctat ccggtgaccg ctgttggcag cgtggcggaa gacaaccttc
    22741 gcgaactggg tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa tttccgggca
    22801 ctgtccatgt ggcaggccct gtacccgacg atatcgcgcc gggatcggtt ttgaagtttg
    22861 aatctgttaa ggagtaaaaa atgaatcagg ttgccgttgt catcggtggt gggcaaacct
    22921 taggcgcgtt cctgtgccac ggtctggctg ccgaggggta tcgcgtcgcg gttgtcgata
    22981 ttcagagcga caaagccgca aatgtggcac aagaaattaa cgccgaatat ggtgaaggaa
    23041 cagcgtatag ttttggtgct gacgccacca gcgagcaaag cgtcctggcg ctctctcgtg
    23101 gggtagatga aatctttggg cgcgtggatt tgctggtcta cagcgccgga atagcaaaag
    23161 cagcctttat cagcgacttc cagctcggcg attttgaccg tgaatctggt gggttatttc
    23221 ctgtgtgccc gtgaattttc tcgtttgatg atccgcgacg ggattcaggg tcgcattatt
    23281 cagatcaact cgaaatccgg caaagtgggc agcaaacaca actctggcta cagcgcagcg
    23341 aaatttggtg gcgtcgggct gactcaatca ctggcgctgg atctggcgga gtacggcatt
    23401 acggtgcatt cactgatgct cggtaacctg ctgaaatcgc ctatgttcca gtcactgtta
    23461 ccccaatacg cgaccaagct gggtatcaaa ccggatcaag tcgagcagta ttacatcgac
    23521 aaagtaccgc tcaaacgcgg ctgcgattat caggatgtgc tgaatatgct gctgctctat
    23581 gccagtccta aggcgtcgta ctgcaccgga cagtcgatca atgtcaccgg cggtcaggtg
    23641 atgttgtaat caacagcgga gatccgttaa ggatctccgt gagactaata gaatgcctga
    23701 tgcgctacgc tcatcaggca tacaggactt ccgccactac attaaggaaa agttatggta
    23761 tccgcactca tcaccgtcgc cgttatcgcc tggtgtgcgc aactggcctt aggcggctgg
    23821 caaatttctc gttttaaccg tgccttcgac acactatgcc agcaagggcg ggttggcgtg
    23881 ggccgttcca gcgggcgctt taaaccacgg gtcgtggtcg ccatcgccct ggacgatcag
    23941 cagcgcatcg tcgacacctt gtttatgaaa ggactgaccg tcttcgcacg accgcaaaaa
    24001 attcccgcaa ttaccggtat gcatgcgggt gatttacagc ccgatgtgat ctttccccat
    24061 gatccactat cacagaatgc tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt
    24121 tgtgaatgtt acttgcttgc gaagttatca ttttgaaacc taaatcaggt aatcacgccc
    24181 atgaaacctc gtcagcgtca ggccgccatt ctggagtatc tgcaaaagca gggtaaatgc
    24241 tcggttgaag aattggcgca atactttgac accacaggca caaccattcg caaagatctg
    24301 gtcattctgg aacatgcagg aaccgtcatt cgtacttatg gcggagtggt gttgaataaa
    24361 gaggaatccg atccgcctat cgatcataaa acactcatca acacccacaa gaaagagctg
    24421 atagcagaag ctgccgttag ttttattcat gatggcgatt cgatcattct tgatgctggc
    24481 agtaccgttt tgcagatggt tcccctgctc tcgcgcttta ataacatcac ggtgatgacc
    24541 aacagtctgc atatcgtcaa tgcgctatct gaactggata acgaacaaac tatcctgatg
    24601 ccaggcggaa cgtttcgtaa aaaatcggcc tcatttcacg gacagctggc agagaatgcc
    24661 ttcgaacatt tcagtttcga taaattgttt atgggcaccg acggcatcga tctcaatgcg
    24721 ggcgtaacca cctttaacga ggtttatacc gtcagtaagg caatgtgcaa tgccgcgcgc
    24781 gaagtgattt tgatggcgga ctcatcaaag tttggccgta aaagccccaa cgtagtttgc
    24841 agtcttgaaa gcgtcgataa gctgattacc gacgcaggta tcgatccggc gtttcgtcag
    24901 gcgctggaag agaaagggat cgatgtgatc ataaccggag agagcaatga gtgaagcact
    24961 actgaacgcg ggacgtcaga cgttaatgct ggagttgcag gaagcaagcc gtttaccgga
    25021 acgtctgggc gatgattttg ttcgcgccgc caatatcatc ctgcactgcg aaggcaaagt
    25081 ggtggtttcg ggaattggca aatcgggcca cattggtaag aaaatcgccg caacacttgc
    25141 cagtaccggc acatcggctt tttttgtcca tccggcagaa gcactgcacg gcgatctggg
    25201 gatgattgaa agccgcgatg tgatgctgtt tatctcttac tccggtggcg cgaaggaact
    25261 ggatctgatt attccgcgtc tggaagataa atctatcgcg ctgctggcga tgaccggcaa
    25321 accgacgtca ccgctgggcc tggcggcaaa agcggtgctg gatatctccg tagaacgcga
    25381 agcctgcccg atgcaccttg cgccgacctc cagcaccgtc aataccctga tgatgggtga
    25441 cgcgctggcg atggcggtca tgcaggcgcg cggatttaat gaagaagatt ttgcccgctc
    25501 ccacccagcc ggggcactgg gcgctcgctt gctgaataaa gtgcatcatc tgatgcgccg
    25561 tgacgatgcc atcccacagg tggcgttaac cgccagcgtg atggatgcga tgctggaact
    25621 cagccgcacc ggtctggggc tggtggcggt atgtgacgct caacaacagg tacaaggcgt
    25681 ctttaccgac ggcgatttac gtcgctggct ggtaggcggc ggcgcactca ccacgccagt
    25741 taatgaagcg atgacgacag gcggcaccac gttacaggcg caaagtcgcg ccatcgacgc
    25801 caaagagatc ctgatgaagc gcaaaatcac tgccgcaccg gtggtggatg aaaacggcaa
    25861 actcaccggc gcaattaacc tgcaggattt ctatcaggcc gggattattt aatccttcat
    25921 tcccagacgt ttcgccagcc gatgcaggtt ggcgacgtcg gtttccagca tccgcgcgca
    25981 ggcagcccag ttatgatgat tttgtgccag tgcctggcga atagtttcac gctggaacgc
    26041 ttctgtcgct tcacgcaggt
//
LOCUS       DAZOYL010000022        25625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZOYL010000022 DAZOYL010000000
VERSION     DAZOYL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.022"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            3..167
                     /gene="yqiD"
                     /locus_tag="ACHUOY_000678"
     CDS             3..167
                     /gene="yqiD"
                     /locus_tag="ACHUOY_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR0681543.1"
                     /translation="MAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYLNS
                     TREETAEKVE"
     gene            complement(225..998)
                     /gene="zupT"
                     /locus_tag="ACHUOY_000679"
     CDS             complement(225..998)
                     /gene="zupT"
                     /locus_tag="ACHUOY_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR0681544.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            1141..1929
                     /gene="ygiD"
                     /locus_tag="ACHUOY_000680"
     CDS             1141..1929
                     /gene="ygiD"
                     /locus_tag="ACHUOY_000680"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR0681545.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(1967..3127)
                     /locus_tag="ACHUOY_000681"
     CDS             complement(1967..3127)
                     /locus_tag="ACHUOY_000681"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0681546.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(3133..3804)
                     /locus_tag="ACHUOY_000682"
     CDS             complement(3133..3804)
                     /locus_tag="ACHUOY_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR0681547.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(3952..5433)
                     /gene="tolC"
                     /locus_tag="ACHUOY_000683"
     CDS             complement(3952..5433)
                     /gene="tolC"
                     /locus_tag="ACHUOY_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR0681548.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            5638..6267
                     /gene="nudF"
                     /locus_tag="ACHUOY_000684"
     CDS             5638..6267
                     /gene="nudF"
                     /locus_tag="ACHUOY_000684"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR0681549.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6268..6690
                     /locus_tag="ACHUOY_000685"
     CDS             6268..6690
                     /locus_tag="ACHUOY_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR0681550.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            6715..7542
                     /gene="cpdA"
                     /locus_tag="ACHUOY_000686"
     CDS             6715..7542
                     /gene="cpdA"
                     /locus_tag="ACHUOY_000686"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR0681551.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            7542..8123
                     /gene="yqiA"
                     /locus_tag="ACHUOY_000687"
     CDS             7542..8123
                     /gene="yqiA"
                     /locus_tag="ACHUOY_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR0681552.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            8152..10044
                     /gene="parE"
                     /locus_tag="ACHUOY_000688"
     CDS             8152..10044
                     /gene="parE"
                     /locus_tag="ACHUOY_000688"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR0681553.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(10092..10406)
                     /locus_tag="ACHUOY_000689"
     CDS             complement(10092..10406)
                     /locus_tag="ACHUOY_000689"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR0681554.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(10437..11018)
                     /gene="mdaB"
                     /locus_tag="ACHUOY_000690"
     CDS             complement(10437..11018)
                     /gene="mdaB"
                     /locus_tag="ACHUOY_000690"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR0681555.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            complement(11128..12477)
                     /gene="qseC"
                     /locus_tag="ACHUOY_000691"
     CDS             complement(11128..12477)
                     /gene="qseC"
                     /locus_tag="ACHUOY_000691"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR0681556.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(12474..13133)
                     /gene="qseB"
                     /locus_tag="ACHUOY_000692"
     CDS             complement(12474..13133)
                     /gene="qseB"
                     /locus_tag="ACHUOY_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR0681557.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            13285..13677
                     /gene="ygiW"
                     /locus_tag="ACHUOY_000693"
     CDS             13285..13677
                     /gene="ygiW"
                     /locus_tag="ACHUOY_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR0681558.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            13730..14212
                     /locus_tag="ACHUOY_000694"
     CDS             13730..14212
                     /locus_tag="ACHUOY_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR0681559.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            14758..17016
                     /gene="parC"
                     /locus_tag="ACHUOY_000695"
     CDS             14758..17016
                     /gene="parC"
                     /locus_tag="ACHUOY_000695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR0681560.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            17249..17986
                     /gene="plsC"
                     /locus_tag="ACHUOY_000696"
                     /gene_synonym="parF"
     CDS             17249..17986
                     /gene="plsC"
                     /locus_tag="ACHUOY_000696"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR0681561.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            18061..19473
                     /gene="ftsP"
                     /locus_tag="ACHUOY_000697"
     CDS             18061..19473
                     /gene="ftsP"
                     /locus_tag="ACHUOY_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR0681562.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            19584..21803
                     /locus_tag="ACHUOY_000698"
     CDS             19584..21803
                     /locus_tag="ACHUOY_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR0681563.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21846..22103)
                     /gene="yqhH"
                     /locus_tag="ACHUOY_000699"
     CDS             complement(21846..22103)
                     /gene="yqhH"
                     /locus_tag="ACHUOY_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR0681564.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22154..23080)
                     /locus_tag="ACHUOY_000700"
     CDS             complement(22154..23080)
                     /locus_tag="ACHUOY_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR0681565.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            complement(23280..24107)
                     /gene="dkgA"
                     /locus_tag="ACHUOY_000701"
     CDS             complement(23280..24107)
                     /gene="dkgA"
                     /locus_tag="ACHUOY_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR0681566.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            complement(24212..25375)
                     /gene="yqhD"
                     /locus_tag="ACHUOY_000702"
     CDS             complement(24212..25375)
                     /gene="yqhD"
                     /locus_tag="ACHUOY_000702"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR0681567.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
BASE COUNT         5935 a         6498 c         6881 g         6311 t
ORIGIN      
        1 ttatcgcctg gtactggatt gtattgattg ctctggttgt ggtgggttat ttcctgcatt
       61 tgaaacgtta ttgtcgggcg tttcgccagg acagagacgc actgcttgaa gcgcggaaca
      121 aatacttaaa cagtacgaga gaagagacag ccgaaaaggt agaataaggc tttcgtgaag
      181 ctggcagaag ttgtccatgt gctggacaac ttctgatgtt gtttttaacc aattcccgcc
      241 gtttgtagca gcactaaact gaatcccatc actgacattc cacatagtac gccatagctg
      301 gggttattat tagggtcaat ctctttggcg agcggcatta attcatcaac cgagagcgcc
      361 accataattc ctgcaaccgc cgccatgatt gccgccatga ccaccgggga aatcatgcta
      421 ccgaggatta accacgccag cacaccacca agaatttctg ctagtccaga aatccccgcc
      481 cacagaatcg cggtgcgttt agaccccgtt gccgcataaa ccgggcctgc cactgccaga
      541 ccttcaggga tattgtgcaa cgcgacggcc agtgcgatgc caaatcccag ctccaggttg
      601 ctgctcgccg tgacaaaggt ggcaatccct tccgggaagt tatgcagact gatgccgaga
      661 gtgagcagaa tggctgtgcg cttgatcgat tttggcaacg gctgcaccga tttttgcatt
      721 aaatcctgcg gatgcgcatg tggcaacatg cggtccaggc caaaatagcc aagcagaccg
      781 aagataaaca ttccataacc caacacaggc gacattcctt cagctgctag tgcggcagga
      841 agcatttcca ttaatgagat gagcaacatg atccccgccg caaatcctag cgaaaacgcc
      901 agtaagcggt tcgagggttt ttgcccgaga acgccgagaa acgcgccaat aaacgtggct
      961 gcccccgcca gtatggtcag aatgagaggt actgacatcg acaactcctt ataaatcttc
     1021 accttcaggc agatcgtcaa aataactgat gatcatcatc attgttatcc gggttcttac
     1081 tccgccgaac aagcgtattg tgaggatatc aaaatgacac ctttagttaa ggatatcatc
     1141 atgtcttcaa cacgtatgcc agcattgttt ttaggtcacg gtagtccgat gaacgtgctg
     1201 gaagataatt tgtatacccg cagctggcag aagttgggga tgacattgcc acgcccgcaa
     1261 gcgattgtgg tggtttcggc tcactggttt acccgtggaa caggagtgac cgcgatggag
     1321 acgccgccca cgattcatga ctttggtggc ttcccgcagg cgctgtacga tacgcattat
     1381 cctgctccgg gttcgcctgt gctggcacag catctggttg agctgttagc gtcggtcccg
     1441 gtgacgctgg ataaagaagc ctggggcttt gaccacggtt cctggggcgt gctgattaag
     1501 atgtatcctg acgccgatat cccgatggtg cagttgagta tcgacagtag caaacctgcc
     1561 gcctggcatt tcgaaatggg gcgcaaactg gcagcgctgc gagatgaagg aataatgttg
     1621 gtcgccagtg gcaacgtggt gcataacctg cgtacagtga agtggcacgg tgatagttca
     1681 ccatatcctt gggcgatgtc gtttaatgag tatgtgaaag cgaatctgac gtggcaaggg
     1741 gcggtagagc agcatcctct ggtgaattac cttgaccatg aaggtggcgc gttatcgaac
     1801 ccaacgccgg agcactatct gccgttgttg tatgtcttag gtgcgtggga tgggcaggag
     1861 ccaattacca ttccggtcga tggtatagaa atgggcagcc tgagtatgct gtcggtgcag
     1921 ataggttaac aattaccgga tggcgaatgc catccggcat cattgattac tcaataaaaa
     1981 tatgtggata aaaccgagac atatcctggg tgatcaacgc acggtcttca cgaatgccaa
     2041 ttccggcagg ttgatcgttc accagccagc taccaatcag catatagctg tcgccgaatt
     2101 tcggtaacgg gtggaattgc tgaacaatca tcccttcttc gccatacgga ccttccgctg
     2161 cttcaatggt tttgccgttc tcaatgatcg acacgtttgc gccttcacgg gagaagatcg
     2221 gtttaaccac atatttttcc atttgcggat gatcatcttc cgcaaaataa gcgggcagca
     2281 ggttcgggtg attcgggaac atctcccata gtagcggtag aagcgctttg ttggagataa
     2341 tgctcttcca cgccggttcc agccagcgta cgcctgcatc ctccagcttg gtggagaaca
     2401 tctcacgcaa cataaattcc catggataca gcttgaacag gttggaaatt acctgatcct
     2461 gtaaatccgt gaactgacct ttttcaccta acccgatatc gtcaatgtag aggaactcgg
     2521 tagcgatttc agcttccgtc gcgcagtcct gcaaatactg aatggttccg cgatcttcca
     2581 ccgtgtcgcg acagcaggtg agatgcagca actggaagcc atactgttca cgcaactcga
     2641 caaagcgatc gattagcttt tcttgcagac tgttaaactg gtcgctgccc tccggcaagt
     2701 taccggcgtt aagctgatct tccagccaga tccactgaaa gaacgccgcc tcgtatagtg
     2761 acgttggtgt atcggcgtta ttttccagca gtttaggttc gccagtgcca tcccatgcca
     2821 gatcaagacg cgaataaagc gatggctggt gagtcagcca tgactgacgc acaaaactcc
     2881 aggtgcgttt tggaatgcgg aatttggtca tcagctcatc gctggcgatc actttttcca
     2941 ccactttcag gcacatctgg tgcagttcgg cggtgacttc ttccagcttt tcaacctggg
     3001 cgagggtcaa cttgtagtaa gcatcttcac accagtacgg ctcgccgtac atggtgtgaa
     3061 aattgaaacc gtattcgtgg gctttttcgc gccagtccgg gcgctcggta atactgactc
     3121 tttccatcgg tatcagccac ccattgaacg agaagaggtg ccggttgcgc tacgctgcat
     3181 agtgctttgt ttggcaacag attcaccaaa accgccacgg gtaacggtag tagtggtcgc
     3241 cggttttggt gccatggctg tcttcggtac ggtcatggtg cggcctggct gggctgcgcc
     3301 atagttttta cccgtcgcgt cggtatattt accgtaagcc gggctggctg ggtttttcga
     3361 ggagaacagc ggctgctgtg caaatcccgc gccgccgccc atcagacgcc ccatcatgta
     3421 accggccatc agcggcatcc agaaactccc gctggattgc tgggcctgcg cctggttttc
     3481 tggtgccatg ccagcctggg ctggtgcctg ctggcactga ccttcaccaa attcagcaac
     3541 acagtcttca cgggtggcgt atttcggcgc agtacgttcg gcttctttca gcgcattgtt
     3601 gtacgcggtg gtacattcgg cgcttttgcc tgggtttgca gctgaacagt cgtcagcatt
     3661 ttgatagaga gacactgttt catcactctt ttcacagcca gccagcataa aaacagtggc
     3721 aaccgcgaga gcgactggtg tcagatggcg tgcgctccag tttttgcgga acgatgcgtg
     3781 gcgtatggat tttgtccgtt tcatttttgt cttccgggac cagtggtaaa tacccatcag
     3841 aatagaggat ggctggtcga aattgaagcg agaaggggga agaatgcggc agataacccg
     3901 tatctttacg ttgccttacg ttcagacggg gccgaagccc cgtcgtcgtc gtcagttacg
     3961 gaaagggtta tgaccgttac tggtggtagt gcgtgcggat gtttgctgaa cgactggtgc
     4021 cgggctatca ggcgcataac catcagcaat agcattctgt tccggcgttt gcggtgcaac
     4081 gttttccgga ttagtggaaa ccggtttgct cagcgcattg ttcagtgcca gcagatcctg
     4141 ctcgttcaac gtacccagag ctgacttaat attcaactgg ttaatcaggt agttataacg
     4201 cgcatttgcc agctcttgct tagcgttgta cagcgtggtg gttgcatcca acacatcaac
     4261 aatggtacgc gtaccgaccg agtagcccgc ttccatcgcg tctaatgagc tttgagcgga
     4321 aactacggct tgtttgtagg cgttaatgct actgatagat gcattaatgt tgttgaagga
     4381 ggaacgtacg gtttgcacga cgctacgatg cgcgctttcc agttgctcgc tggcaccaac
     4441 aaagttgtac tgggcctgtt tcacctgcga gttaaccatt ccgccctgat aaatcggcag
     4501 cgagaagctc aggcccactt tgttctggcc catattgctg tcgtcatact gggtaccagc
     4561 ggcaccacgg gttttcgaac cgctataaga ggtgtcagaa atcccggtag aagccgttaa
     4621 atccagcgtc ggtaagtgac catcctgcgc ctggcgaatt tgctcgcgcg ccaggtcctg
     4681 gctcaagcgt gcctgtaaca gcgacaggtt gcgtttttcg gcttctttca gcagcgcgtt
     4741 aaccggctgt ggtttgtcgg ttttaaagtt ttcgacattc agcgccgcca gttccggata
     4801 gtagttaccg gtgatctggc gcagctgctc taccgcgtta tcaaggttat tacgtgcggt
     4861 cacttcgttc gccagcacgg tatcgtactg cgcgcgggcg ttctgcacgt cggtgatcgc
     4921 taccaggccc acgttaaaac gttgggtggt ttgatctaat tgacggtaga tcgcttcttt
     4981 ttgtgcctgt gtataggaaa gaacgtcaat agcattcaac acgttgaaat aagcggtcgc
     5041 ggtgttgagg atcaaggttt gctgatcggt ctgatatgtg acgtcctgaa tccctgctgc
     5101 tttttcctgc agcgttaacg cacgccattt cgacatatca aaaatggatt gagttaactg
     5161 cagggacgca ctggtcgcgt tagagttgat gccgttcgcg tcgcggtagc cgttgctata
     5221 ggtgtaatct gcacctaaac ctagctgtgg cagtaatgga ctgcgcgctt cattaatttt
     5281 ttcaaaggca gcatcacgat cggcggcaga cttacgcaat tccgggttac taaggcgtgc
     5341 ttgctgataa acttgcatca ggttctcggc ctggctcaac gaactgaacc cagaaaggct
     5401 caggccgata agaatgggga gcaatttctt catttgcatt ccttgtggtg aagcagtatt
     5461 tagcgcgatc aaactgtaaa attattgcgg attctagcag aagccgctac cgcaaaaggt
     5521 tggcgttacg tgccataggg cgttaatgtg cctcaattta acacatgact gctcaaacgg
     5581 cagtcaaacg tcgctgaaat tcacatttaa ttcactatta gtgccaggac attaacaatg
     5641 cttaagccag acaacctgcc cgttacattt ggcaaaaacg atgtagaaat tattgcacga
     5701 gaaacacttt atcgcggctt tttttcatta gatctttata gatttcgtca tcgtctattc
     5761 aacgggcaaa tgagtcatga ggtacggcgg gaaatttttg agcgcggtca cgccgcagtc
     5821 ttgctaccct ttgacccagt gcgcgatgaa gttgtgctga ttgagcagat tcggattgcc
     5881 gcatacgaca ccagtgaaac cccctggcta ctggagatgg ttgccgggat gatagaagag
     5941 ggtgaaagtg tggaagatgt tgcccgtcgc gaagcgattg aagaggcggg actgatagtc
     6001 aaacggacca aaccggtgtt aagtttcctg gcaagcccgg ggggcaccag tgagcgttcg
     6061 tcaattatgg tgggcgaagt ggacgccacg accgcaagcg gtattcatgg tctggctgat
     6121 gaaaacgaag atattcgcgt tcatgtggta agccgggaac aggcatacca gtgggtagaa
     6181 gaggggaaaa tcgacaacgc agcgtcggtc atcgctttgc aatggctgca gctgcatcat
     6241 caagcgttaa aaaatgagtg ggcataaatg aagcgttaca cacctgactt tcctgaaatg
     6301 atgcgcctgt gcgagatgaa cttttcacaa ttgcgccgtt tgttaccgcg caatgacgca
     6361 cccggcgaaa ctgtaagcta tcaggtggca aacgcacaat atcggctgac gattgtggaa
     6421 tcgacccgat acactaccct ggtgacaata gaacagactg cgcccgcgat cagttactgg
     6481 agccttccgt caatgacggt gcgtctgtat catgacgcga tggtggctga agtgtgttca
     6541 agccagcaga tttttcgctt caaagcgcgg tatgattatc ctaataaaaa gttgcatcaa
     6601 cgcgacgaaa agcatcaaat taatcagttt ttagcggact ggttgcgata ctgtttagca
     6661 catggagcga tggcgattcc ggtttactag catcgtgaaa cctaaggaca ccatttggaa
     6721 agcctgttaa cccttcctct ggctggtgag gccagagtca ggattttaca aattaccgac
     6781 actcacctgt ttgcacaaaa gcacgaagct ctgttagggg taaacacctg ggaaagctac
     6841 caggcggtgc tggaggcgat tcgtccacat cagcacgaat tcgacctgat tgtcgcgaca
     6901 ggtgatttag cgcaggatca atcctctgcg gcctatcagc atttcgctga aggcatcgca
     6961 agttttcgtg cgccctgcgt ctggctgccg ggcaaccacg atttccagcc cgcgatgtac
     7021 agcgcgttac aggatgcggg tatctccccg gcgaagcggg tgtttattgg tgagcaatgg
     7081 caaatcctgt tgctggatag ccaggtgttt ggcgtgccgc acggtgagct gagcgagttt
     7141 cagcttgagt ggctggaacg taaactggcc gatgcgccag aacgccatac gttgctgctg
     7201 ctgcatcatc atccgctacc tgcgggttgt agttggctcg atcaacacag tctgcgtaac
     7261 gcgggcgaac tggataccgt gctggcgaag tttccgcacg tcaaatactt gctgtgcggt
     7321 catattcatc aggagctgga tctcgactgg aacggtcgcc gcctgctggc aacgccgtcg
     7381 acctgtgtgc agtttaagcc gcactgttcc aactttacgc tggatactat cgcgcccggc
     7441 tggcgtactc tcgagttaca tgctgatggc acgctgacca ccgaggtgca tcgcctggcg
     7501 gacacacgtt tccaacctga taccgcttca gaaggctact gatgtctacg cttctttatt
     7561 tacacggttt caacagctcg ccgcgctctg caaaagcgag cttgttaaaa aactggctgg
     7621 cggaacatca ccctgacgtt gagatgatca ttccgcagtt gccgccgtat ccttccgacg
     7681 cggcagaact gctggaatcc attgtcctgg aacatggcgg cgattcgctg ggtattgtcg
     7741 gttcgtcact ggggggatat tacgccacct ggttgtcaca atgttttatg ctgcccgcag
     7801 tggtggtaaa cccggcggtg cgcccgtttg aactgctgac ggactatctc ggtcagaacg
     7861 agaacccata caccgggcag caatatgtgc tagagtcacg ccatatttac gatctcaaag
     7921 tcatgcagat tgacccgctg gaagcgccgg atttgatctg gctgctgcaa cagacgggag
     7981 atgaagtgct ggattaccgc caggcggtgg cgtactacgc ttcctgccgc cagactgtca
     8041 tagaaggcgg caaccacgca ttcacgggct tcgaagatta tttcaacccg atcgtcgatt
     8101 ttcttggtct gcaccatctc tgacgatcaa ctcgaattgc taacttaaac catgacgcaa
     8161 acttataacg ctgatgccat tgaggtactc accgggcttg agccggttcg ccgccgtccg
     8221 gggatgtata ccgataccac tcgccctaac catttggggc aagaagttat tgataacagt
     8281 gtcgatgaag cactggcggg tcacgcaaaa cgcgtggatg taatcttaca tgctgaccag
     8341 tcgttagaag ttattgacga tgggcgcggg atgccggtgg atattcaccc ggaagagggt
     8401 gtcccggcgg ttgaattgat tctttgccgt ctgcatgcag gcggtaaatt ctctaacaaa
     8461 aattaccagt tctctggcgg cctgcatggc gtggggattt cggtggttaa cgccctgtcg
     8521 aagcgcgttg aagttaacgt gcgccgcgat ggtcaggttt ataacatcgc ctttgaaaat
     8581 ggcgaaaagg tgcaggattt acaggttgtc ggcacttgcg gtaaacgcaa taccggtacc
     8641 agcgtgcact tctggccgga tgaaaccttc tttgacagcc cgcgattttc tgtttcacgc
     8701 ctgacgcatg tgctgaaagc caaagcggta ctgtgccccg gcgttgagat tacctttaaa
     8761 gatgagatca acaacaccga acagcgctgg tgctatcagg acggtctgaa tgattacctg
     8821 gcggaagcgg taaacggttt accgacgctg ccagaaaaac cgtttatcgg taatttcgct
     8881 ggcgatactg aagctgtgga ctgggcgcta ctgtggctgc cggaaggcgg tgaactgctg
     8941 accgaaagct acgtcaacct gatcccaacg atgcagggcg gtacccatgt taatggcctg
     9001 cgtcagggcc tgttggacgc gatgcgtgag ttctgtgaat atcgcaacat tctgccgcgc
     9061 ggcgtgaagc tgtcggcgga agatatctgg gatcgctgcg cctatgttct gtcggtgaaa
     9121 atgcaggatc cgcagtttgc cgggcagacg aaagagcgtc tctcttcgcg tcaatgcgcg
     9181 gcattcgttt ctggcgtggt gaaagatgcc tttatcctgt ggctgaacca gaacgttcag
     9241 gcggcggagt tgctggcaga gatggcgatt tccagcgccc agcgtcgtat gcgtgcggct
     9301 aaaaaagtgg tgcgtaaaaa gctgaccagc ggcccggcgc tgcctggcaa actggctgat
     9361 tgtaccgcgc aggaccttaa ccgtaccgaa ctgttcctcg tggaaggtga ctccgcaggc
     9421 ggatctgcca agcaggcgcg cgatcgtgaa tatcaggcga tcatgccgct gaaaggtaag
     9481 atccttaaca cctgggaagt ttcttccgac gaagtgctgg cctcgcagga agtgcatgat
     9541 atttcggtag cgatcggtat cgatcctaac agcgacgatc tgagccagct tcgttatggc
     9601 aaaatctgta tcctcgcgga tgcggactct gatggtctgc acattgccac gctgctctgc
     9661 gctttgttcg taaaacactt ccgcgcgttg gtgaaacacg gtcacgttta cgtcgcactg
     9721 ccaccgctct accgtattga tctcgggaaa gaggtttatt acgcgctgac ggaagaagag
     9781 aaagagggcg tacttgagca attaaaacgc aagaaaggca agccgaacgt ccagcgtttt
     9841 aaaggtctgg gggaaatgaa cccgatgcaa ttgcgtgaaa ccacgcttga tccgaacact
     9901 cgccgtctgg tgcagttgac tatcgatgat gaagacgatc agcgtactga cgcgatgatg
     9961 gatatgctgc tggcgaagaa acgctcggaa gatcgccgca actggttgca agagaaaggc
    10021 gacatggcgg agattgaggt ttaaaggaaa gaacattgcc cggtcaggat ggccgggcaa
    10081 acaaggcagg attaaatccc tggctgcaga atacgaatat tcatctccag cacgtcgcct
    10141 tttacggctt cgctgtacgc cttcatgtgc ggggtttgca gatgcgcttc aaggtgcgcg
    10201 atgctttccc actgctcaat catcacgata gaatccggtg ccatagactg gaaactcacg
    10261 ccagcagcgc aatccaccat tggcgcatag ccgtggcaac cttcttcttt cagtacggtt
    10321 ggaacgattt tagcaaactg atccaatacc gcctgacggt gatgttgacc aggacgagta
    10381 cggatttctg cgattacggt aagcatggtt aactccttct aaagcctgag ctctagttaa
    10441 ccaaaaattt ccacaagatg cttgcgatat tcttcagtat agcggggaac atcaggcatt
    10501 tttatcacgt cattagcgat aaatgtcggc agcggttcca tgccaaggaa ttggtttgct
    10561 ttatggaacg gcagatacac accgtcgacg ccaacgccgt ggaagaattg atctttttcg
    10621 gtgaaggctt ccattggtgc gttccaggtc agagaaagca tatatttttt gccctgtacc
    10681 aggccgccgg aaccgtattt ttttgacgga tctttgcggg tacggccatc gctggcatac
    10741 agcgtcccgt gaccttcggt gaatacatca tcaatgtatt ttttcactgt ccacggcgcg
    10801 cccatccacc agcctggcat ctgccagatc accacatcag cccagagaaa gttttgtact
    10861 tccgctttga catcgtagtc gctgtcggcg cgaacgatgc gaacatcatg cccgaggtcg
    10921 cgcagtgtgc catccgcgac ttcggtcagg gtgtcgttca gttgaccatt ggagtgggcg
    10981 aattttttcg cgccgttgat aatcagggtg ttgctcattt tttaacctca aggtgagacg
    11041 tttgtcgact atagtacgtc acagagcggt acggtaaaat tagcaaaatg tgcaaagtct
    11101 tttgcgaatt tagcaaaagt cttaccctta ccagctcacc ttcgcctcaa atccaccttg
    11161 ttccgcattc ccaaattcaa cattcatgcc atgcaatttg gcgattcgct ggacaatcga
    11221 tagcccaagt ccactgccgg tagcggtttg tccggggggg cgatagaagc gttcgccaat
    11281 tcgcgccagt gcctctggtg tcacaccggg gccgttatcc ctcacgatga aattatcggc
    11341 attcagcgtg acgtccacca cgctgccctg tggactgtag cgcactgcgt tatccagaag
    11401 gtttcgcacc aacaaactta gcaatagcgg ttgtccagtg cgtttgatgc tgtgggcatt
    11461 gagtgtcagt cgcacgtcaa ttttcgcctg ctgcgccgtg tggtaaatat ccatcaccga
    11521 cgattgcagg agatcttcaa gcgggatctc cgcgacgtcc tgaaggttat ccagtgagtc
    11581 cagccgcgat agcgtgagca gttgatcaac cagacgagta gcgcgatcga tcccggaatg
    11641 taattggagc agtgcttttt tccgcgcctg cggatcatcg tcagagagct gcgcaacttc
    11701 ggtttgcact ttcagcgctg ttaacgggct acgtagttcg tgagctgcat cggaggtaaa
    11761 gcgtcgttct cgaaccatca tcgcatgtgt gcgggcgaac agttgattta gcgactcaac
    11821 cagtggtcgc acttcgctgg gtacgccagt cgcgtttagt ggtttttccg agtcagggtc
    11881 acgcatacgt agtgccagcg ccagtttgtt cagcggcgcg agttcacgac ccagtagtac
    11941 catcatgatg attaacataa tcggcagtgc gaccagccac gggatcaatt gcccggcaac
    12001 aatcgccagc gccatgtctt cacggtattc ccattcctgg ccaacaacga tgcgatattt
    12061 gccatcaggt gaggtcatcc agacaaaacg ccaaggatcg tcttcaccga ccagttgccc
    12121 gtcagcaaaa ccttcccgtt gatagctata gggaatatct tctccgttat cgccatcatt
    12181 aaggaccatt ctgccgtcgt gggtaaagat ggcaaaggtc agcgcatcgt catcaacatg
    12241 accgtgtttt aatttatttg gcgtctgtgc catgcgatcc gccgcgttga tttcgttgag
    12301 atcgagcgta cttaaccgct tggcaaacag catcagttgg gtgtcgaaca attcatcgac
    12361 gttatccgtt gtttgtttcc aggcgacaaa gctggaaagc agccaggtca ccgaggccag
    12421 aattaaaaag attagcgtca gcctgacgcg cagactaaga cgttgggtaa atttcatttc
    12481 tcacctaatg tgtagccaat accatgcacg gtacgaataa aatcactacc gagtttgcgt
    12541 cgcagatgat gcacatgcac ttcaacggca ttactggtga cctcttcgtc ccaggtatac
    12601 agtttctctt caatcagttt gcgcggcagt acccgaccag cgttacgcat cagtaattcc
    12661 agcagggcaa attcttttgg tttcagtgtt aagggttcgc cagccagcgt ggcgatacgt
    12721 ttgccggggt cgagcatgac gttaccgtgg cgcagctcgt tgctggcctg gccgttggtt
    12781 cggcgcatca gagcctccag cctggcggcg acttctatca acgcaaaagg tttacacaga
    12841 taatcgtcag ctcccagacg cagcccttct acacgttccg ctaacgcatc acgcgcggtc
    12901 aggatcagta ccggctcacg ctgacctttc tctcgccatt cgcgcaaaat atcgcgacca
    12961 tccatgcctg gtaaggttaa atccaggatc accgcatcat aaggtgcgct ataaagcgcc
    13021 tcttttccct gacgaccttg tgtaaaccag tcgacactaa aacccatttt actaaggcca
    13081 gttttgatgc cgtcgccaat cagcatgtca tcttctatca gtaaaattcg cattttttca
    13141 tccctgcgat aaccgtattt gctgccagta aaccgtgttg taacgcgctc tgtacagcat
    13201 taaattttat ttttttccct taagaagttg ttaaggacta tcttgttaaa tgctcggaaa
    13261 cagacattaa agggagtaat aaacatgaaa aaattcgcag cagtaatcgc agtaatggcc
    13321 ctgtgcagtg caccggtgat ggcagcagag cagggcggtt tttctggccc atcggcaacg
    13381 caaagtcagg ccggaggatt ccaggggccg aacggcagcg taacgactgt agaaagcgca
    13441 aaatccctgc gtgacgacac ctgggtaacc ctgcgcggca atatcgttga acgcatctct
    13501 gacgatctct acgtgtttaa agatgccagc ggtactatca atgttgatat cgaccacaaa
    13561 cgctggaacg gtgtgacggt gacgccgaaa gatacggttg agattcaggg tgaagtcgat
    13621 aaagactgga attctgttga aattgacgtc aaacagatcc gcaaagtaaa tccgtaattg
    13681 ttaccgctcc cgggacgcgt tcccgggaat aatttcgcag ggaggcaaaa tgacaaacct
    13741 gacactggat gtaaacatta tcgatttccc atcaatccct gtggcgatgt tgccgcaccg
    13801 ctgtagccct gagttgctca actacagtgt ggcgaaattt atcatgtggc gtaaagagac
    13861 ggggctttct cctgttaacc aaagccagac ttttggcgtc gcctgggacg accctgccac
    13921 caccgcaccg gaagcgtttc gctttgatat ctgcggcagc gttagcgaac cgattcccga
    13981 taatcgttat ggtgtgagca atggtgaact taccggtgga cgttatgccg tggcccgcca
    14041 cgttggcgag ctggacgata tttcacacac gatatggggc attattcgcc actggctgcc
    14101 tgcaagcggc gagaaaatgc gtaaagcacc gattctgttt cactacacca atcttgccga
    14161 aggggtgacg gagcagcgac tggaaacgga tgtttatgtg ccgttagcgt gatggggaat
    14221 ctttaccgga gtgtgagcca aatcactcgc gataagtttg agcgaacaat gccacaggcg
    14281 tattgttgtt gggcttataa tttctccggt aaggaatacc atgtatacgc gaaatttatt
    14341 atccggtgcg ccgtaagccg tctcgggggt acccatgagc aagccgcact tatggcagga
    14401 ggcgcggggc actggtggag acacagctaa actgggctga accatatact tacagtcgac
    14461 tactttgatg ctttaggctt ccctggactc gcataacctc aacttctcga atcgcccgga
    14521 gcggaccccc atgccgagtg gtgtggcatg ggcgcaacct tgtgctgccc tctatgccga
    14581 tcaagtctac agcaaagcgt tgtatatcaa ggcacattga tgcctttatc gccgtcggag
    14641 cacgtccggc ggcgctattc acgctgatct cctgtgactc gacgcggcag ataatgtagt
    14701 atctccggca atattgcccc tttgaaggct ggcgaatcag ttgaggaatc agaattaatg
    14761 agcgatatgg cagagcgcct tgcgctacat gaatttacgg aaaacgccta cttaaactac
    14821 tccatgtacg tgatcatgga ccgtgcgttg ccgtttattg gtgatggtct gaaacctgtt
    14881 cagcgccgca ttgtgtatgc gatgtctgaa ctgggcctga atgccagcgc caaatttaaa
    14941 aaatcggccc gtaccgtcgg tgacgtactg ggtaaatacc atccgcacgg cgatagcgcc
    15001 tgttatgaag cgatggtcct gatggcgcag ccgttctctt accgttatcc gctggttgat
    15061 ggtcagggga actggggcgc gccggacgat ccgaaatcgt tcgcggcaat gcgttacacc
    15121 gaatcccggt tgtcgaaata ttccgagctg ctattgagcg agctggggca ggggacggct
    15181 gactgggtgc caaacttcga cggcactttg caggagccga aaatgctgcc tgctcgtctg
    15241 ccaaacattt tgcttaacgg caccaccggt attgccgtcg gcatggcgac cgatattcca
    15301 ccgcataacc tgcgtgaagt agctcaggcg gcaatcgcat taatcgacca gccgaaaacc
    15361 acgctcgatc agctgctgga tatcgtgcag gggccggatt atccgactga agcggaaatt
    15421 atcacttcgc gcgccgagat ccgtaaaatc tacgagaacg gacgtggttc agtacgtatg
    15481 cgcgcggtgt ggaagaaaga agatggcgcg gtggttatca gcgcattacc gcatcaggtt
    15541 tcaggtgcgc gtgtactgga gcaaattgct gcgcaaatgc gcaacaaaaa gctgccgatg
    15601 gttgacgatc tgcgcgatga atctgaccac gagaacccga cccgtctggt gattgtgccg
    15661 cgttccaacc gcgtggatat ggatcaggtg atgaaccacc tcttcgctac caccgatctg
    15721 gaaaagagct atcgcattaa cctcaatatg atcggtctgg atggtcgtcc ggcggtgaaa
    15781 aacctgctgg aaatcctctc cgaatggctg gtgttccgtc gcgataccgt gcgccgccga
    15841 ctgaactatc gtctggagaa agtcctcaag cgcctgcata tcctggaagg tttgctggtg
    15901 gcgtttctca acatcgacga agtgattgag atcattcgta acgaagatga accgaaaccg
    15961 gcgctgatgt cgcggtttgg ccttacggaa acccaggcgg aagcgatcct cgaactgaaa
    16021 ctgcgtcatc ttgccaaact ggaagagatg aagattcgcg gtgagcagag tgagctggaa
    16081 aaagagcgcg accagttgca gggcattttg gcttccgagc gtaaaatgaa taacctgctg
    16141 aagaaagaac tgcaggcaga cgcgcaagcc tacggtgacg atcgtcgttc gccgttgcag
    16201 gaacgcgaag aagcgaaagc gatgagcgag cacgacatgc tgccgtctga acctgtcacc
    16261 attgtgctgt cgcagatggg ctgggtacgc agcgctaaag gccatgatat cgacgcgccg
    16321 ggcctgaatt ataaagcggg tgatagcttc aaagtggcgg tgaaaggtaa gagcaaccaa
    16381 tcggtagtgt ttgttgattc caccggtcgt agctatgcca tcgacccgat tacgctgccg
    16441 tcggcgcgtg gtcagggcga accactcacc ggcaaattaa cgttgccgcc tggggcgacc
    16501 gttgaccata tgctgatgga aagcgacgat cagaaactgc tgatggcttc cgatgcgggt
    16561 tacggtttcg tctgcacctt taacgatctg gtggcgcgta accgtgcagg taaggctttg
    16621 atcaccttac cggaaaatgc ccatgttatg ccgccggtgg tgattgaaga tgcttccgat
    16681 atgctgctgg caatcactca ggcaggccgt atgttgatgt tcccggtaag cgatctgccg
    16741 cagctgtcga agggcaaagg caacaagatt atcaacattc catcggcaga agccgcgcgt
    16801 ggagaagatg gtctggcgca attgtacgtt ctgccgccgc aaagcacgct gaccattcat
    16861 gttgggaaac gcaaaattaa actgcgcccg gaagagttac agaaagtcac tggtgaacgt
    16921 ggacgccgcg gtacgttgat gcgcggtttg cagcgtatcg atcgtgttga gatcgactct
    16981 cctcgccgtg ccagcaacgg tgatagcgaa gagtaatatg ccggggaggg cctgtttcct
    17041 cccgcttgca aggaacgccg gatgaaacgt taagtcgtca tccggcagcg tattcaaccc
    17101 ccggaaacga taaaaattcc cttgaaaccg ttgtttattc atgcgttgcg attaacaata
    17161 cgcttttcca gagagcggct tttaacaatg ccctaacctg atttcaggtg acgtacaatg
    17221 ccagtctctc agaccttcag agggtgttat gctatatatc tttcgtctta ttattaccgt
    17281 gatttacagc atcttagtct gtgtattcgg ctccatttac tgccttttca gcccgcgtaa
    17341 cccgaaacat gtggccacct ttgggcatat gtttggccgt cttgcgccgc tgttcggcct
    17401 gaaagttgag tgccgcaaac ctgcagatgc tgaaagctac ggcaatgcta tctatatcgc
    17461 taaccaccag aacaactatg acatggtgac agcatcgaac attgtgcaac cgccgacggt
    17521 gacggtaggt aaaaagagct tgctgtggat ccccttcttc gggcagttgt actggttaac
    17581 cggcaactta ttgatcgaca gaaacaatcg cactaaagct cacggcacca ttgcggaagt
    17641 cgtgaatcat ttcaaaaaac gccgtatttc catctggatg ttcccggaag gaacccgcag
    17701 ccgtggtcgc ggcctgctac cgttcaagac tggagcattt cacgcggcaa ttgcggcggg
    17761 cgtcccgatt attcccgtgt gcgtctctac aacttcgaat aagattaatc ttaatcgact
    17821 gcacaacggt ctggtgattg tcgaaatgct gccgccaatt gacgtcagtc agtatggcaa
    17881 agatcaggtt cgtgagctgg ctgcccattg tcgttcgata atggaacaaa aaatcgccga
    17941 gctcgataaa gaagtcgcag aacgcgaagc cgccggaaaa gtttaagtcg gcaatctgta
    18001 tttttgcggg gaacactttc ctgcacggta ttactttagc cagttttaca tggagcaaat
    18061 atgtcactca gtcggcgtca gttcattcag gcatcgggga ttgcactttg tgcaggcgct
    18121 gttcccctga aggccagcgc agccgggcaa cagcaaccgc tacccgttcc gccgctactt
    18181 gaatctcgcc gtgggcaacc gctgtttatg actgtacaac gtgcgcactg gtcatttacg
    18241 ccagggacac gcgcgtcggt ctggggaatc aatggtcgtt acctggggcc gactatccgc
    18301 gtctggaagg gcgacgatgt taagcttatt tacagcaacc gcctgacaga aaatgtctca
    18361 atgacggtgg ccgggctaca ggtacctggc ccgctgatgg gcggtccggc acggatgatg
    18421 tcgccaaacg ctgactgggc acccgtactg cctattcgcc agaacgcagc tactctgtgg
    18481 tatcacgcca atactcccaa ccgcacggct cagcaggtct ataacggcct tgccggaatg
    18541 tggctggtgg aagatgaagt cagcaagtcg ctgcctatcc ccaaccatta tgatgtggat
    18601 gattttccgg tcattatcca ggataaacgg ctggataact ttggtacgcc agaatacaac
    18661 gaaccgggaa gcggcggctt tgttggtgat acgctgctgg ttaacggtgt acaaagcccg
    18721 tacgttgaag tctcgcgtgg ctgggtgcgc ttgcgactgc tgaacgcgtc gaactctcgt
    18781 cgctatcaac tacagatgag cgatggtcgc ccgttacatg tgatttctgg cgatcaggga
    18841 ttcctgcctg ctcctgtatc ggtgaagcaa ctttcgctgg caccgggcga gcgccgcgag
    18901 attctggtgg atatgagcaa cggcgatgaa gtgtcgatca cctgtggcga agcggcgagc
    18961 attgttgatc gtattcgcgg cttctttgag ccatccagta ttctggtttc taccctggtg
    19021 ctaacgctgc gcccaaccgg ccttctgccg ctggtcacag acagtcttcc gatgcgcttg
    19081 ctgccaactg aaatcatggc cggttcgccg attcgcagtc gcgatatcag tctgggtgat
    19141 gacccgggta ttaatggaca gctgtgggac gtcaaccgta ttgatgtcac cgcgcagcaa
    19201 ggaacgtggg aacgctggac ggtacgcgcg gacgagccgc aagcgttcca tattgaaggc
    19261 gtgatgttcc agatccgtaa cgtgaatggt gcgatgccgt tcccggaaga cagaggctgg
    19321 aaagataccg tttgggttga cggacaagtg gagctgcttg tttatttcgg tcagccttcc
    19381 tgggcgcact tcccgttcta cttcaacagt cagacgctgg aaatggcgga ccgtggctcg
    19441 attgggcaac tgttagtcaa tccggtaccg taatcttcat actcgcccga ggagggcgag
    19501 tatggaatac ttttcgattt ctctttcgtt ccggcttata atccccggcc tttgattatt
    19561 tttttatcat ttctggaagc aaaatgagct ctatctccct gatccaaccg gatcgcgacc
    19621 tgttctcctg gccgcagtac tgggccgcct gttttggacc ggcaccgttt ttgccgatgt
    19681 ctcgtgaaga gatggatcaa cttggctggg atagctgcga catcattttg gttactggcg
    19741 acgcgtatgt cgatcaccca agcttcggga tggcgatttg cggtcgtatg ctggaagcac
    19801 agggctttcg cgtcgggatc atcgcccagc cggactggag cagcaaagac gactttatgc
    19861 gtctgggtaa accgaatctg tttttcggtg ttactgctgg caacatggat tcgatgatca
    19921 accgttatac cgccgatcgc cgtttacgtc atgacgatgc ctacacgccg gataacgtcg
    19981 cgggtaagcg cccggatcgc gccacactgg tttataccca gcgttgtaaa gaggcgtgga
    20041 aagatgtacc ggtgatcctc ggcggtattg aggctagtct gcgccgtacc gcacattatg
    20101 attactggtc cgataccgtg cgccgttccg tgctggtgga ttcgaaagcc gacatgctga
    20161 tgtttggtaa cggtgagcgt ccgctggtgg aggtggcgca ccgtctggcg atgggcgagc
    20221 cgattagtga aatccgcgat gtgcgtaata ccgcgattat cgtgaaagag gcgctgcctg
    20281 gctggagcgg cgtggattcc ccccgtcttg atacccctgg aaaaatcgac ccaatcccgc
    20341 atccgtatgg tgaagatttg ccgtgcgcgg ataacaaacc ggtggcaccg aaaaagcagg
    20401 aagccaaagc cgtaaccgtg cagccaccgc gcccgaaacc gtgggaaaaa acctacgtgt
    20461 tgctgccttc tttcgagaaa gtgaagggcg ataaagtgct gtacgcccat gcttcgcgta
    20521 ttctgcacca cgaaaccaac ccaggctgcg tccgcgcatt gatgcaaaaa cacggtgacc
    20581 gctatgtgtg gatcaacccg cctgctatcc cgctttctac cgaagagatg gacagcgttt
    20641 ttgcgctgcc gtacaagcgc gtgccacatc cggcctatgg caatgcccgt attccggctt
    20701 acgaaatgat ccgtttttcg gtcaacatta tgcgcggctg ctttggcggc tgttctttct
    20761 gttctattac cgagcacgaa gggcgcatta ttcagagccg ttcggaagat tcgatcatta
    20821 atgagatcga agcgatccgc gacaccgttc caggttttac gggcgtgatt tccgatctcg
    20881 gtgggccaac tgccaacatg tatatgttgc actgcaaatc gccacgcgct gaacaaactt
    20941 gtcgccgttt gtcgtgcgtt tatccggata tttgtccgca catggacact aaccacgaac
    21001 cgacgatcaa cctctatcgc cgtgcgcgtg atctgaaagg cattaaaaaa atcctcatcg
    21061 cctcaggcgt acgttatgac atcgccgtgg aagatccgcg ctatatcaaa gagttggcga
    21121 cccatcacgt cggcggttat ctgaagattg ctccggaaca taccgaagaa gggccgttgt
    21181 cgaagatgat gaagccgggc atgggcagct atgaccgctt taaagagctg ttcgatacct
    21241 actcgaaaca ggcaggtaaa gagcagtatc tgatcccgta tttcatctct gcgcaccccg
    21301 gtacgcgtga tgaagatatg gtgaatttgg cgctgtggct gaaaaagcat cgtttccgtc
    21361 tcgaccaggt acagaacttc tacccatcgc cgctggcgaa ctcaaccacc atgtattaca
    21421 ccgggaaaaa accgctggcg aagattggtt ataagagcga agacgtcttc gtaccgaagg
    21481 gcgacaaaca gcgtcgtttg cataaagcgt tgttgcgtta ccacgatccg gcaaactggc
    21541 cgttaatccg cccggcgctg gaagcgatgg gcaaaaagca tctgattggc agccgtcgcg
    21601 attgcttagt gcctgcgcca accattgaag agatgcgtga agctcgtcgc cagaaccgca
    21661 atacccgtcc ggcgttgact aaacatacgc cgatggcgac ccagcgtcag acgcctgcta
    21721 cggcaaaaaa agcgtcgtct acgcaatctc gtccggtgaa tgctggtgcg aagaaacgcc
    21781 ctaaagcggc ggttggacgt taaaaaaaat ccccggcaac tgacatgctg ccggggattt
    21841 ttttatcatt ctgcgtacat acgcaagcag cgcaggcaat caaaatagtc ctgagcatca
    21901 agacgcgtat tggctctgtt agcttcggat ttggcagcat agatttgtgg gcgtagtgct
    21961 tttatatcct gctcaagccg ggcgattttg gcatttaatg tttgtacatt gctcgccagt
    22021 tgattgacct tttgctcatt gacgcagcca ctgagcaagc aggttgccag aacaacagct
    22081 cccacggtga aaatcgtttt catatagtgt tcccttttat ttattgttcc ggctaattgt
    22141 tatttaattc agctcattgc tttaatctgg cggcggtttt tgcctcaatc tgcttaagta
    22201 ggctgccgct gttcgggcgg ataaaatcag cgatttccca cttgccttcg cgccgctgta
    22261 aaaccagttc ttgctcttcg ttgttgccat tggtcagcgt aaaacgaaca tgaatgcggg
    22321 cggaatcggc ggttttctcc aggactcgaa tttcatcaac gcgtgaaaat agcgactctt
    22381 cgccaaaaca gatcggattg tcgtaaataa attgccgttc tatttgcata tcttcatcgg
    22441 gattattggc cgtcttgaag gtggttttat agaaggcatt aaccgcctgg cggtaagagt
    22501 cggaaaccac ccacgtccac ggccagctat aatcagcaat atgttcaaag agttcggcaa
    22561 caaaggcttc cggctgttct ttttgcaacg aagctaaagc ggccagcgtt ttttcattct
    22621 cgctattaac tgcttgtaag acgctgccat gattgctgac aatatcgtca atgacccaac
    22681 gaccattttc cgccaccatt ttcagtgtct gcgtggtctt ttctttatca tctttaaaga
    22741 tacgaaagcg cacaacggca tcggcatgat cggcgtcagt ttgggttatc gcaacgcttt
    22801 ctagcaccag atcgccaaaa tcctgacaat cacaaaccgg atcataatcc agccagccaa
    22861 tattgcccgg cagcgtaaga ttgtcgttca gggtaagcgc ctgttgaata cgcgctgaag
    22921 ttatcgcccg ctctccggtt tcaccgaaat aaggtgtact ggcatctgat ttatagttct
    22981 gataaatctg ccggactgtt tgttctacgg tctgtgaagg aggctgcgcg tgtacggtaa
    23041 aacttgccag ggctgctaaa aacagaagta ttattttcat ctgtttttca cctctccgtt
    23101 gcgtttatcc cggatataga tgagttgttc ttttaagagc ttccggctct gcatgatgat
    23161 gtccttatat ttggcattcc tgaacaagga agagtaacaa cgggcgggac gcgaggggaa
    23221 taaatgattt ctgaaaagtc cggtagcgga acattaccgt caccgggaga atttgcatgt
    23281 tagccgccga actggtcagg atcgggaccg agacgcttgc cctgatcgag ttttgcaatt
    23341 tcgccgagtt cgtctttgtc gagacggaaa tcccagacat caaagttttc ggcaatacgt
    23401 gaaggtgtga ccgatttcgg gatcaccacc aggccgctat ccagatgcca gcggataaca
    23461 atctgcgccg gggctttgcc gtatttatct gccagatcgc gaatgacttt ctgatcgaaa
    23521 acgcctttcc ctccttgcgc taatgggctc caggattcgg tctggatttt gtgtgtcgcg
    23581 ttccaggcgt gtagctggcg ttgttgcatc agcggatgaa gttcgatctg gtttatcaca
    23641 ggcgtcacgc cagtttcatc aatcaggcgt tgcaggtgat ggatctggaa gttgcacacg
    23701 ccgatgcttt tgattaatcc ctctttttgc aattcgatca tgcctttcca tgcttcgaca
    23761 taatggtcga tagcgggaac gggccagtgc attaagtaga ggtcgatata atcaagctgg
    23821 agttttttca ggctgtcgag cagggcttcg cgggggcgct tgtggtcgtc gttccacagc
    23881 ttagtggtga tgaacagttc ttctctgttg actgaggcat ttttcagggc tttgccgaca
    23941 ccttcttcgt tcttgtaggc cgcggcggta tcaatcgagc gataacccac ttctaacgct
    24001 ttttgaatgg cggtgattac ttcctcatta cttgcttgcc agacgcccag tcccagctgg
    24061 ggcatgacat tgccatcctg tagcttaata acggttggat tagccatacg ttcctccttt
    24121 atatgaactc accggagcag gctccggtga ggtgttgtga acttaagtct ggacgaaatg
    24181 cccgaaaacg aaagtttgag gcgtaaaaag cttagcgggc ggcttcgtat atacggcggc
    24241 tgacatccaa cgtaatgtca tgattttcgc ccagttgggt catgccgtgc tcttccagtt
    24301 ttttcagcaa agccgggatg gagctgccgt ccagaccgta gtcggagagg tgggtcggca
    24361 cgcctaattg ctcaaagaaa ttgcgggttg cggcaatcgc ggcgtcaata cgctcatcgt
    24421 cggaaccttc agtgatgttc cagacgcgtt cagcatattg cagcagctta gcgcgcttgg
    24481 tctcgcgttt ttcattccac agtgcaggca ggacgatagc cagtgtttgc gcgtgatcca
    24541 gaccgtgcat cgcagtcagt tcgtggccca gcatatgcgt tgcccagtcc tgcggtacgc
    24601 cagcgccaat caaaccgttc agcgcctgag tcgccgccca catgacgttg gcgcgcacat
    24661 cgtagttttc tggctctttc agggctttcg gaccatcttc gattagcgtc agcaaaatgc
    24721 cttctgcgaa acggtcctga attttggcat caaccggttt ggtaacatac tgttccacgg
    24781 tgtgtacaaa ggcgtccact acgtcgttag ccacctgacg cggcggcagg gtgtaggtat
    24841 aaaccggatc gagcacggca aataccggct gaacatgggc agaatggaac gcctgcttgt
    24901 cgcctgtggt tttacgggag atcaccgcgc ctgcgttgga ttctgaaccg gttgctggca
    24961 gcgtcagcac acagcccatc gggatggcgc ttttaatctc tttaccgccc gtttgcagaa
    25021 tgtgccacgg atcgatattt tccggatagt tagccgctgc ggcgataaat ttggtgccgt
    25081 ccagtacaga accgccgcca accgccagca ggaaagtcac tttctgttcg cgaaccagtt
    25141 tcacggcgtt catcagcgtt tcataagccg ggtttggctc aataccgcca aattccagca
    25201 cgtccatgcc tttcagggca tccagaactt gatcgagaac gccgattttt ttcacgctgc
    25261 cgccgccgta ggtaatcaat acgcgagcat cgtgaggaat ttgttcgcgt aaaccagcga
    25321 ttgcgccttt accaaacaga atgcgggttg gggtgtgcag attaaagttg ttcattactt
    25381 gctccctttg ctgggccaat atgagggcag agaacgatct gcctgatgtt tttcattgtg
    25441 atcgccagcg ccctggctct caatgctcat ttctgccaat gtcttgccta tttctccaga
    25501 gtgctggaga aatgctacaa aattgcgcac aatcaaattg ccgcattatt cctaagaaat
    25561 tacgcgatat gaaacgtgaa gagatttgcc gcttgctggc ggataaagtt aataaactga
    25621 aaaat
//
LOCUS       DAZOYL010000023        25494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZOYL010000023 DAZOYL010000000
VERSION     DAZOYL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.023"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..485
                     /gene="gspA"
                     /locus_tag="ACHUOY_000703"
     CDS             <1..485
                     /gene="gspA"
                     /locus_tag="ACHUOY_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR0681568.1"
                     /translation="SLQTLVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTW
                     TLTHKWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAE
                     WRPLLVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK
                     "
     gene            487..906
                     /gene="gspB"
                     /locus_tag="ACHUOY_000704"
     CDS             487..906
                     /gene="gspB"
                     /locus_tag="ACHUOY_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFR0681569.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1144..1455
                     /gene="rpsJ"
                     /locus_tag="ACHUOY_000705"
     CDS             1144..1455
                     /gene="rpsJ"
                     /locus_tag="ACHUOY_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR0681570.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1488..2117
                     /gene="rplC"
                     /locus_tag="ACHUOY_000706"
     CDS             1488..2117
                     /gene="rplC"
                     /locus_tag="ACHUOY_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR0681571.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2128..2733
                     /gene="rplD"
                     /locus_tag="ACHUOY_000707"
     CDS             2128..2733
                     /gene="rplD"
                     /locus_tag="ACHUOY_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR0681572.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2730..3032
                     /gene="rplW"
                     /locus_tag="ACHUOY_000708"
     CDS             2730..3032
                     /gene="rplW"
                     /locus_tag="ACHUOY_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR0681573.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3050..3871
                     /gene="rplB"
                     /locus_tag="ACHUOY_000709"
     CDS             3050..3871
                     /gene="rplB"
                     /locus_tag="ACHUOY_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR0681574.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3888..4166
                     /gene="rpsS"
                     /locus_tag="ACHUOY_000710"
     CDS             3888..4166
                     /gene="rpsS"
                     /locus_tag="ACHUOY_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR0681575.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4181..4513
                     /gene="rplV"
                     /locus_tag="ACHUOY_000711"
     CDS             4181..4513
                     /gene="rplV"
                     /locus_tag="ACHUOY_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR0681576.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4531..5232
                     /gene="rpsC"
                     /locus_tag="ACHUOY_000712"
     CDS             4531..5232
                     /gene="rpsC"
                     /locus_tag="ACHUOY_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR0681577.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5245..5655
                     /gene="rplP"
                     /locus_tag="ACHUOY_000713"
     CDS             5245..5655
                     /gene="rplP"
                     /locus_tag="ACHUOY_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR0681578.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5655..5846
                     /gene="rpmC"
                     /locus_tag="ACHUOY_000714"
     CDS             5655..5846
                     /gene="rpmC"
                     /locus_tag="ACHUOY_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR0681579.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5846..6100
                     /gene="rpsQ"
                     /locus_tag="ACHUOY_000715"
     CDS             5846..6100
                     /gene="rpsQ"
                     /locus_tag="ACHUOY_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR0681580.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6265..6636
                     /gene="rplN"
                     /locus_tag="ACHUOY_000716"
     CDS             6265..6636
                     /gene="rplN"
                     /locus_tag="ACHUOY_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR0681581.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6647..6961
                     /gene="rplX"
                     /locus_tag="ACHUOY_000717"
     CDS             6647..6961
                     /gene="rplX"
                     /locus_tag="ACHUOY_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR0681582.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6976..7515
                     /gene="rplE"
                     /locus_tag="ACHUOY_000718"
     CDS             6976..7515
                     /gene="rplE"
                     /locus_tag="ACHUOY_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR0681583.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7530..7835
                     /gene="rpsN"
                     /locus_tag="ACHUOY_000719"
     CDS             7530..7835
                     /gene="rpsN"
                     /locus_tag="ACHUOY_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR0681584.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7869..8261
                     /gene="rpsH"
                     /locus_tag="ACHUOY_000720"
     CDS             7869..8261
                     /gene="rpsH"
                     /locus_tag="ACHUOY_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR0681585.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8274..8807
                     /gene="rplF"
                     /locus_tag="ACHUOY_000721"
     CDS             8274..8807
                     /gene="rplF"
                     /locus_tag="ACHUOY_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR0681586.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8817..9170
                     /gene="rplR"
                     /locus_tag="ACHUOY_000722"
     CDS             8817..9170
                     /gene="rplR"
                     /locus_tag="ACHUOY_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR0681587.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9185..9688
                     /gene="rpsE"
                     /locus_tag="ACHUOY_000723"
     CDS             9185..9688
                     /gene="rpsE"
                     /locus_tag="ACHUOY_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR0681588.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9692..9871
                     /gene="rpmD"
                     /locus_tag="ACHUOY_000724"
     CDS             9692..9871
                     /gene="rpmD"
                     /locus_tag="ACHUOY_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR0681589.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9875..10309
                     /gene="rplO"
                     /locus_tag="ACHUOY_000725"
     CDS             9875..10309
                     /gene="rplO"
                     /locus_tag="ACHUOY_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR0681590.1"
                     /translation="MRLNTLSPAEGSKKTGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10317..11648
                     /gene="secY"
                     /locus_tag="ACHUOY_000726"
     CDS             10317..11648
                     /gene="secY"
                     /locus_tag="ACHUOY_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR0681591.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11680..11796
                     /gene="rpmJ"
                     /locus_tag="ACHUOY_000727"
     CDS             11680..11796
                     /gene="rpmJ"
                     /locus_tag="ACHUOY_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR0681592.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11943..12299
                     /gene="rpsM"
                     /locus_tag="ACHUOY_000728"
     CDS             11943..12299
                     /gene="rpsM"
                     /locus_tag="ACHUOY_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR0681593.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12316..12705
                     /gene="rpsK"
                     /locus_tag="ACHUOY_000729"
     CDS             12316..12705
                     /gene="rpsK"
                     /locus_tag="ACHUOY_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR0681594.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12739..13359
                     /gene="rpsD"
                     /locus_tag="ACHUOY_000730"
     CDS             12739..13359
                     /gene="rpsD"
                     /locus_tag="ACHUOY_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR0681595.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13385..14374
                     /gene="rpoA"
                     /locus_tag="ACHUOY_000731"
     CDS             13385..14374
                     /gene="rpoA"
                     /locus_tag="ACHUOY_000731"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR0681596.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14415..14798
                     /gene="rplQ"
                     /locus_tag="ACHUOY_000732"
     CDS             14415..14798
                     /gene="rplQ"
                     /locus_tag="ACHUOY_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR0681597.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14905..15273
                     /locus_tag="ACHUOY_000733"
     CDS             14905..15273
                     /locus_tag="ACHUOY_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR0681598.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15284..15709
                     /gene="zntR"
                     /locus_tag="ACHUOY_000734"
     CDS             15284..15709
                     /gene="zntR"
                     /locus_tag="ACHUOY_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR0681599.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15765..15983
                     /gene="arfA"
                     /locus_tag="ACHUOY_000735"
     CDS             15765..15983
                     /gene="arfA"
                     /locus_tag="ACHUOY_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR0681600.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="ACHUOY_000736"
     CDS             complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="ACHUOY_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR0681601.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="ACHUOY_000737"
     CDS             complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="ACHUOY_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR0681602.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="ACHUOY_000738"
     CDS             complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="ACHUOY_000738"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR0681603.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="ACHUOY_000739"
     CDS             complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="ACHUOY_000739"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR0681604.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20215..20724)
                     /gene="def"
                     /locus_tag="ACHUOY_000740"
     CDS             complement(20215..20724)
                     /gene="def"
                     /locus_tag="ACHUOY_000740"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR0681605.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20854..21978
                     /gene="dprA"
                     /locus_tag="ACHUOY_000741"
     CDS             20854..21978
                     /gene="dprA"
                     /locus_tag="ACHUOY_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR0681606.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21950..22423
                     /gene="smg"
                     /locus_tag="ACHUOY_000742"
     CDS             21950..22423
                     /gene="smg"
                     /locus_tag="ACHUOY_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR0681607.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22452..22994
                     /locus_tag="ACHUOY_000743"
     CDS             22452..22994
                     /locus_tag="ACHUOY_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR0681608.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22999..23571
                     /gene="tsaC"
                     /locus_tag="ACHUOY_000744"
     CDS             22999..23571
                     /gene="tsaC"
                     /locus_tag="ACHUOY_000744"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR0681609.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23576..24394
                     /gene="aroE"
                     /locus_tag="ACHUOY_000745"
     CDS             23576..24394
                     /gene="aroE"
                     /locus_tag="ACHUOY_000745"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR0681610.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24391..24648
                     /locus_tag="ACHUOY_000746"
     CDS             24391..24648
                     /locus_tag="ACHUOY_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR0681611.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24624..25178)
                     /locus_tag="ACHUOY_000747"
     CDS             complement(24624..25178)
                     /locus_tag="ACHUOY_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR0681612.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6493 a         6023 c         6678 g         6300 t
ORIGIN      
        1 cttctttaca aacattggta gatcagaatt tgccctggag cgcatcgctt aaagtcgggg
       61 acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag
      121 gtcagcaaac gtggacgctg acacataaat ggtttgagtc ggtatggacg ggtgattatc
      181 ttctgttgtg gaaaatgtcg ccggaagggg agagtaccat tacgcgtgat agcagcgagg
      241 aagagattct ctggctggaa acgatgttaa accgtgcatt gcatatttcg acagaacctt
      301 cggcggagtg gcgtcccttg ttggtagaaa aaataaagca atttcaaaaa agccaccact
      361 tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca tctctggcag gtggcagggg
      421 agagtgccta cttatatcgg gatgaagcaa atatttcccc ggaaacaaca gtgaagggga
      481 aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt
      541 atcttctccc gacaaaaaca cagcaccata atatatgtca tttgtttatt gctgatttgc
      601 ctgtggtttg caggaatggt gttggttggt gggtatgcca ggcagctatg ggcgctttgg
      661 atagtaaaag cagaagtcac tgtagaggct gaaacgcccg cttttaaaca atcaacacaa
      721 cactatttct tcaaaaaaca gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac
      781 ccagacgtcg cggttgagaa tgctccctct tcttctgaag acgaagaaaa tactgtaaaa
      841 gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa atgcgctgaa cgaacttgag
      901 agataacccg aaggctgttt actttactag caatacgctt gcgttcggtg gttaagtatg
      961 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1021 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1081 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1141 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1201 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1261 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1321 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     1381 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     1441 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     1501 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     1561 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     1621 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     1681 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     1741 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgaggtta
     1801 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     1861 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     1921 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     1981 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2041 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2101 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2161 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2221 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2281 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     2341 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     2401 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     2461 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     2521 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     2581 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat
     2641 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     2701 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     2761 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     2821 tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     2881 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     2941 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3001 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3061 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3121 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3181 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3241 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3301 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     3361 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     3421 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     3481 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     3541 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     3601 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     3661 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     3721 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     3781 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     3841 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     3901 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     3961 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4021 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtgaccgacg
     4081 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4141 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca
     4201 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4261 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt
     4321 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     4381 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     4441 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     4501 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     4561 cgcttgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct
     4621 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     4681 tctgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     4741 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     4801 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     4861 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     4921 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     4981 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5041 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5101 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5161 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5221 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5281 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     5341 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     5401 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     5461 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     5521 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     5581 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     5641 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca
     5701 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     5761 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     5821 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     5881 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     5941 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6001 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6061 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6121 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6181 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6241 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6301 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     6361 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     6421 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     6481 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     6541 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     6601 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     6661 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     6721 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     6781 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     6841 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     6901 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     6961 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7021 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7081 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7141 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7201 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7261 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtattcg tgacttccgt
     7321 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     7381 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     7441 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     7501 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     7561 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     7621 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     7681 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgcccgc
     7741 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     7801 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     7861 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     7921 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     7981 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8041 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8101 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8161 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8221 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8281 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     8341 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     8401 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     8461 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     8521 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     8581 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     8641 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgt tgataagcag gtgatcggcc
     8701 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc
     8761 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     8821 ataagaaatt tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     8881 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     8941 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9001 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9061 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9121 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9181 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9241 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9301 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
     9361 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
     9421 cactctgcaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
     9481 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
     9541 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
     9601 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
     9661 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
     9721 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg
     9781 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
     9841 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
     9901 cgaaggctcc aaaaagacgg gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa
     9961 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10021 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10081 taaagcagcg attacagccg aagttcgtct gtctgacctg gctaaagtag aaggcggtgt
    10141 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10201 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10261 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10321 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    10381 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    10441 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    10501 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    10561 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    10621 tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgct
    10681 acggtactct ggtgctggca atattccagt cgatcggtat tgctactggt ctgccgaata
    10741 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    10801 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatt actgaacgag
    10861 gtatcggcaa cggtatttca atcattatct tcgtcggtat tgtcgcggga ctcccgccag
    10921 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    10981 tggttgcagt attagtattt gcagtgacgt tctttgttgt atttgttgag cgtggtcaac
    11041 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtttat gctgcacaga
    11101 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11161 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11221 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11281 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    11341 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    11401 cggcgaagta tatcgataaa gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta
    11461 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    11521 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    11581 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    11641 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    11701 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    11761 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    11821 agttgggttg agctggctag attagtcagc caatcttttg tatgtctgtg cgtttccatt
    11881 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    11941 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12001 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12061 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12121 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12181 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12241 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12301 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    12361 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    12421 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    12481 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    12541 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    12601 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    12661 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    12721 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    12781 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    12841 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    12901 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    12961 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13021 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13081 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13141 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13201 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacctggct ggaagttgat
    13261 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13321 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    13381 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    13441 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    13501 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    13561 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    13621 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    13681 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    13741 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    13801 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    13861 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    13921 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    13981 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14041 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14101 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14161 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14221 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14281 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcctgga
    14341 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    14401 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    14461 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    14521 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    14581 agactgatag cgttgctaat cgtcgtctgg catttgcccg tactcgtgat aacgagatcg
    14641 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    14701 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    14761 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc
    14821 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    14881 ttttgttcat cccctggagt atttatgtgg ttacttgacc agtgggcaga gcgccatata
    14941 gcagaagcgc aagcgaaagg tgagtttgat aacctggcag gtagcggcga accattgata
    15001 ctggatgatg attctcacgt gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat
    15061 gccggttgct taccgccaga acttgagcaa cggagagaag caattcagct tctggatatt
    15121 ctcaaaggta tccgtcacga tgatccgcaa tatcaagagg ttagccgtcg attgtcatta
    15181 ctggaattga agctgcgaca agctggattg agtaccgatt ttttacgcgg cgattatgct
    15241 gacaagttgt tggacaaaat caacgataac tagtggagta tgtatgtatc gcattggtga
    15301 gctggcaaaa atggcggaag taacacctga cacgattcgt tattacgaaa aacagcagat
    15361 gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg tataccgaaa gcgatctcca
    15421 gcgattgaaa tttatccgcc atgccagaca actaggtttc agtctggagt cgatccgcga
    15481 gttgctgtcg atccgcatcg atcctgaaca ccatacctgt caggagtcaa aaggcattgt
    15541 gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag ttgcagagta tgcagcgttc
    15601 cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat agcagtgttt attgctcgat
    15661 tcttgaagct cttgaacaag gggcgagtgg cgttaagagt ggctgttgat tttttgcact
    15721 gacaggatta cactcgcgcc gttaaataac caactggagt ttttatgagc cgatatcagc
    15781 atactaaagg gcagataaag gataatgcga tagaagcatt actacatgat cccttattcc
    15841 gacagcgagt agagaaaaat aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg
    15901 gcaatcgggg gaactgggag gccagtggca agaaagtaaa tcactttttt accactggtc
    15961 ttctgctttc aggcgcttgt taagagcggt tattctgctc tttcagcaaa tcacgaattt
    16021 ctgccagtaa tacttcttct ttagttggtg caggtgcggc agctggttct tcttttttcc
    16081 gattcagttt gttgattagc ttaatcgcca taaagatggc aaaggccaca atcagaaaat
    16141 caaagacgtt ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg
    16201 catcgcgtag cgtgacagca aactgtttaa agtcgatccc gccaattaat aagcccagag
    16261 gtggcatgat gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa
    16321 tgacacccac cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa
    16381 taatgctcat gttattctcc ctatgaagcc gacaaccata agtctaacaa atgttaagcc
    16441 attttcctgc caggaatgaa tttaattaat ccttgataat caaagggaaa taataaggcg
    16501 tcattagacg ccttattaat tacaagaaga aagggcttgg ctggaagagt ctttcgacgt
    16561 cggtaataaa ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca
    16621 gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac cgctccaata atcgttcctg
    16681 gcggtagctt gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt
    16741 gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat
    16801 ctgcttttcg cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaattgcaa
    16861 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
    16921 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
    16981 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
    17041 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
    17101 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
    17161 ctgcaccgat attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct
    17221 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
    17281 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
    17341 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
    17401 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
    17461 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
    17521 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
    17581 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
    17641 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
    17701 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
    17761 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
    17821 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
    17881 gaatgataat tttcatcagt ctcgacccgt tatctcatca ctttttgatt agcttagcgt
    17941 aaaagaagcc gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg
    18001 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaagcttta atctgcaggc
    18061 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
    18121 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
    18181 cgcgacgtaa ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg
    18241 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
    18301 caccttgttt cacggtcgcc ttcataccaa ggcgttttaa attgtcgtga acgcgagaga
    18361 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
    18421 gcgttgtttt accgccgggg gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg
    18481 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
    18541 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
    18601 catgcgggaa acctttcatt cctgcttcat ccaacaatgc aagccagctg tcgcgggaat
    18661 gatgcgtacg attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg
    18721 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
    18781 aacgtgcatc actggtatta aactcggcta ataactcttc ttgctgacgc tggaactggc
    18841 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
    18901 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
    18961 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
    19021 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
    19081 gtgctttgtc tttatcagaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
    19141 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
    19201 ttttcataag tataaaaatg cccggtaaga ccgggcttag aagagtggac tatcaggcca
    19261 gacggttgcc cggaacaaac cattcccgac gagaattcag gaggtcttgc gcgctcatcg
    19321 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
    19381 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
    19441 tgaccgacgc tttccagact ttaaccggtt gtccttcaat ttccagccag ctcattggcc
    19501 atgggttgaa agcgcgaatg cagcgttcaa gctgcgctgc cgaaagcgac cagtcaatac
    19561 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
    19621 gtttcgccgt gccgtctgct agttgtttca acgtggtgat tagcccttgt gggccaagct
    19681 ctgccagctt gtcgtacagc gtaccactgg tatcttctgc agtaatcggg caggagagct
    19741 tatagagcat atcaccggtg tctaaaccga catccatttg cataatggtc acaccagttt
    19801 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
    19861 gtgaaccatg aacgttgata cagccaagac gcggcatctc cagcactgct ttaggcagaa
    19921 ttaagccata ggcgacgacg accataacat cagcctgcag atcggcgacc agttgctggc
    19981 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag acctttttcc tcagccagaa
    20041 ctttaaccgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
    20101 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
    20161 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
    20221 ccgggctttc agacgatcta gtttttcaac tttctgacga atacgttgtt gtttcagcgg
    20281 tgacagataa tccataaaca gtttgccgac caggtgatcc atctcatgct gaatacagat
    20341 ggctaacagc ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
    20401 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
    20461 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
    20521 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
    20581 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
    20641 tatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
    20701 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggggt tagaaagatt
    20761 atttcttcta ttctagacaa atccccctct gattgacagc atcactgacc aatcgcaaag
    20821 attgctaagg ctgcttatgg cagggagata aggatggtcg atacagaaat ttggctgcgt
    20881 ttaatgagta tcagcagctt gtacggcgat gatatggtcc gtatagctca ctggctggca
    20941 aaacagtcgc aaattgatgc ggttggattg cagcaaacag ggcttacatt gcggcaggca
    21001 caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg gttggagcaa
    21061 cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact tctggtgacg
    21121 acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag
    21181 cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg acggttattt
    21241 tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg tggaatcgat
    21301 ggtgtggcgc ataaagctgc cttacaggta aatggcgtca gcattgctgt attggggaat
    21361 ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct gcttgaacat
    21421 ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca
    21481 cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg
    21541 cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc agaggcgtga agtttttgcc
    21601 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    21661 gcgattcttg tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg
    21721 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    21781 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    21841 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    21901 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    21961 actaatgtat ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga
    22021 caaacttgaa caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct
    22081 gctatggctg gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc
    22141 ctcggatcct ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag
    22201 ctgccgtggg tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga
    22261 aatggtgata gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa
    22321 atgggtgatc ctgatggtgt tgttcaacat cccggggtgc gaaaatgcgt accagcaaat
    22381 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    22441 cataagttgt tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    22501 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    22561 agtatccggc gtgtgactac gtccgtcttc tgaaatcttc agcggatgga catattgtca
    22621 aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa tctggtatta cgccagggac
    22681 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    22741 ataaaccgga cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc
    22801 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    22861 ccattaactt caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg
    22921 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    22981 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23041 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23101 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    23161 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    23221 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    23281 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    23341 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    23401 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    23461 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    23521 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    23581 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    23641 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    23701 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    23761 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    23821 tggtgcagtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    23881 tggtgtaggc ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat
    23941 tctgcttatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctaga
    24001 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24061 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24121 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    24181 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    24241 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    24301 ggcacaggcg gctcatgcct tccttctttg gcatggcgtt ctgcctgatg tagaaccggt
    24361 catcaaacta ttgcaacagg aattgtcagc gtgaatcagg ccatccagtt tccggatagg
    24421 gaagagtggg acgagaataa aagatgtgta tgttttcctg ctctcgtgaa tggtatgcaa
    24481 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct ttactggagg tacgccagaa
    24541 cagtggttag cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta
    24601 attcaggaac aaagtgaaga tgatcaaggt tgggtctggt taccttgatc cagatattcg
    24661 tccttccatt tcacgtagtt atttgcggaa tagcgtaacc cagccttctc ttcatcactt
    24721 aacgggcgga tctgtttgac gggactaccg agatacagat atccgctctc cagccgttta
    24781 ttttgtggga ccaggctacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    24841 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    24901 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    24961 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25021 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25081 agacgaacgt caccaatcac gacgctgcta tcgtcgatca ttacgcgctg gccgatttgt
    25141 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac tcacctcaac aataaatgat
    25201 ttcctaatga ctttgggtgc attattagcc ttgtgcaagt cttttagtat gcaaaaaagc
    25261 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    25321 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    25381 gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa aaattctgaa attcagggtt
    25441 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaag
//
LOCUS       DAZOYL010000024        25156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZOYL010000024 DAZOYL010000000
VERSION     DAZOYL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25156
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.024"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(133..273)
                     /gene="yjjY"
                     /locus_tag="ACHUOY_000748"
     CDS             complement(133..273)
                     /gene="yjjY"
                     /locus_tag="ACHUOY_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR0681613.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            369..1085
                     /gene="arcA"
                     /locus_tag="ACHUOY_000749"
     CDS             369..1085
                     /gene="arcA"
                     /locus_tag="ACHUOY_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR0681614.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1145..2497)
                     /gene="creD"
                     /locus_tag="ACHUOY_000750"
     CDS             complement(1145..2497)
                     /gene="creD"
                     /locus_tag="ACHUOY_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR0681615.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2555..3979)
                     /gene="creC"
                     /locus_tag="ACHUOY_000751"
     CDS             complement(2555..3979)
                     /gene="creC"
                     /locus_tag="ACHUOY_000751"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR0681616.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3979..4668)
                     /gene="creB"
                     /locus_tag="ACHUOY_000752"
     CDS             complement(3979..4668)
                     /gene="creB"
                     /locus_tag="ACHUOY_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR0681617.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4681..5154)
                     /gene="creA"
                     /locus_tag="ACHUOY_000753"
     CDS             complement(4681..5154)
                     /gene="creA"
                     /locus_tag="ACHUOY_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR0681618.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5365..6234
                     /gene="robA"
                     /locus_tag="ACHUOY_000754"
     CDS             5365..6234
                     /gene="robA"
                     /locus_tag="ACHUOY_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR0681619.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6231..6878)
                     /gene="gpmB"
                     /locus_tag="ACHUOY_000755"
     CDS             complement(6231..6878)
                     /gene="gpmB"
                     /locus_tag="ACHUOY_000755"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR0681620.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6930..7451
                     /gene="yjjX"
                     /locus_tag="ACHUOY_000756"
     CDS             6930..7451
                     /gene="yjjX"
                     /locus_tag="ACHUOY_000756"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR0681621.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(7536..7862)
                     /gene="trpR"
                     /locus_tag="ACHUOY_000757"
     CDS             complement(7536..7862)
                     /gene="trpR"
                     /locus_tag="ACHUOY_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR0681622.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7952..9889)
                     /gene="sltY"
                     /locus_tag="ACHUOY_000758"
     CDS             complement(7952..9889)
                     /gene="sltY"
                     /locus_tag="ACHUOY_000758"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR0681623.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10100..11767
                     /gene="ettA"
                     /locus_tag="ACHUOY_000759"
     CDS             10100..11767
                     /gene="ettA"
                     /locus_tag="ACHUOY_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR0681624.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11928..12035)
                     /gene="nadS"
                     /locus_tag="ACHUOY_000760"
                     /pseudo
     CDS             complement(11928..12035)
                     /gene="nadS"
                     /locus_tag="ACHUOY_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(12074..13306)
                     /gene="nadR"
                     /locus_tag="ACHUOY_000761"
     CDS             complement(12074..13306)
                     /gene="nadR"
                     /locus_tag="ACHUOY_000761"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR0681625.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13327..14709)
                     /gene="radA"
                     /locus_tag="ACHUOY_000762"
     CDS             complement(13327..14709)
                     /gene="radA"
                     /locus_tag="ACHUOY_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR0681626.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14758..15726)
                     /gene="serB"
                     /locus_tag="ACHUOY_000763"
     CDS             complement(14758..15726)
                     /gene="serB"
                     /locus_tag="ACHUOY_000763"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR0681627.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15832..16476
                     /locus_tag="ACHUOY_000764"
     CDS             15832..16476
                     /locus_tag="ACHUOY_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR0681628.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16504..17520
                     /gene="lplA"
                     /locus_tag="ACHUOY_000765"
     CDS             16504..17520
                     /gene="lplA"
                     /locus_tag="ACHUOY_000765"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR0681629.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17552..17815
                     /locus_tag="ACHUOY_000766"
                     /pseudo
     CDS             17552..17815
                     /locus_tag="ACHUOY_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(17976..18695)
                     /gene="deoD"
                     /locus_tag="ACHUOY_000767"
     CDS             complement(17976..18695)
                     /gene="deoD"
                     /locus_tag="ACHUOY_000767"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR0681630.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18775..19998)
                     /gene="deoB"
                     /locus_tag="ACHUOY_000768"
     CDS             complement(18775..19998)
                     /gene="deoB"
                     /locus_tag="ACHUOY_000768"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0681631.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20050..21372)
                     /gene="deoA"
                     /locus_tag="ACHUOY_000769"
     CDS             complement(20050..21372)
                     /gene="deoA"
                     /locus_tag="ACHUOY_000769"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR0681632.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21499..22278)
                     /gene="deoC"
                     /locus_tag="ACHUOY_000770"
     CDS             complement(21499..22278)
                     /gene="deoC"
                     /locus_tag="ACHUOY_000770"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR0681633.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22536..24086
                     /locus_tag="ACHUOY_000771"
     CDS             22536..24086
                     /locus_tag="ACHUOY_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR0681634.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24058..24921
                     /locus_tag="ACHUOY_000772"
     CDS             24058..24921
                     /locus_tag="ACHUOY_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR0681635.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         5961 a         6947 c         6404 g         5844 t
ORIGIN      
        1 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
       61 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc
      121 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
      181 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
      241 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
      301 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
      361 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
      421 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
      481 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
      541 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
      601 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
      661 atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc
      721 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
      781 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
      841 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
      901 aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccacacg
      961 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
     1021 cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt gatctggaag
     1081 attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc
     1141 aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg gctttcattt
     1201 tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg ccggccagcg
     1261 ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg agcagtcccc
     1321 acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg cgccagcctt
     1381 tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg gcgattatcc
     1441 atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa aacatcacca
     1501 atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa
     1561 aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt
     1621 ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg aggaagtttt
     1681 cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc
     1741 tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc aaaaaatcac
     1801 ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag
     1861 gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc ttcaggttct
     1921 gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc
     1981 cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc accacaccaa
     2041 taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg atatttggcg
     2101 cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc
     2161 agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg ccatcaacca
     2221 tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct
     2281 cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca acaagttttt
     2341 gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa tcggcacgtt
     2401 cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca
     2461 gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt gcaatggcag
     2521 caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa
     2581 gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac
     2641 gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg
     2701 gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata ccactaccgg
     2761 tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc gttatacgac
     2821 cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc tgatccagta
     2881 acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg atgttttttt
     2941 ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc acatcaacag
     3001 cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttccacca
     3061 gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc actacttcag
     3121 gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta
     3181 gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc
     3241 gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga tcgggaagag
     3301 gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca atggagcggt
     3361 tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa atggcgctgg
     3421 cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc
     3481 ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca
     3541 ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac tgaccacgca
     3601 acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta tttgccgaat
     3661 cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg cgcactttgt
     3721 taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgagcca
     3781 gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagcaacg
     3841 ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga
     3901 caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca
     3961 accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc gatgagtatt
     4021 aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt taatgtgggt
     4081 atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca ttagttgctg
     4141 gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg
     4201 cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa
     4261 atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac gcagtaaggt
     4321 gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc
     4381 aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg
     4441 taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga tatcaggcaa
     4501 accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa
     4561 gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc
     4621 ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg
     4681 ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac
     4741 aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac
     4801 gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc
     4861 tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatagc
     4921 cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg ttttcgcccg
     4981 gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt
     5041 gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc
     5101 caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt
     5161 accattttta tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag
     5221 aacaaaatct caatactttt atttccgtca agccctaaaa catactctac taagaaaaaa
     5281 acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg
     5341 ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc
     5401 tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt
     5461 tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg
     5521 tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg
     5581 attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc
     5641 aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt
     5701 ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc
     5761 ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc
     5821 tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg
     5881 accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac
     5941 gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg
     6001 acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg
     6061 ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc aatgctcaac
     6121 ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga cgccaaagcg
     6181 ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc
     6241 agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacgacccag
     6301 ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga
     6361 cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt
     6421 gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc
     6481 agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg
     6541 tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg
     6601 atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg
     6661 tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta
     6721 ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc
     6781 tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac
     6841 tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg
     6901 taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg accaccaatc
     6961 ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc
     7021 atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc agtgaggaaa
     7081 cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt
     7141 tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg gtggtgattg
     7201 aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct gcggtgattc
     7261 tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat accggtattg
     7321 atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg
     7381 ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc
     7441 aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg
     7501 atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag caacacctct
     7561 tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc
     7621 gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc gccacgcaac
     7681 agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc
     7741 aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa
     7801 cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc
     7861 ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg tactcgctaa
     7921 agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc
     7981 tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa gccagcacgt
     8041 tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc acagcgtcga
     8101 tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg ttataagctg
     8161 ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg taactggtgc
     8221 caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa ccaggaatag
     8281 agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg ccgctggccc
     8341 ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat
     8401 agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg taagccagcg
     8461 ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga acgctaagat
     8521 cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt tttgacttgc
     8581 tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag tacatcaact
     8641 cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc tgcggcgctt
     8701 tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc gggtagaaac
     8761 cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca
     8821 gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc atcggcagac
     8881 gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt cgcacacggc
     8941 gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat ttcgcctgct
     9001 cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct
     9061 gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt gcattttcag
     9121 catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta aaatcggtcg
     9181 ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg
     9241 ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggccag
     9301 tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga tcttgtttac
     9361 ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta
     9421 gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat
     9481 agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc
     9541 cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg
     9601 gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca
     9661 gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc ttcagtccag
     9721 gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg atttgcgcgt
     9781 aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac
     9841 agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct
     9901 ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc taaccagtca
     9961 ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt gatcgaccaa
    10021 ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca tcacgataaa
    10081 catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt
    10141 tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa
    10201 aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca ttatggcggg
    10261 cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttacct
    10321 gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg aagaagcggt
    10381 ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc
    10441 ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga tcattcaggc
    10501 tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc tacgtctgcc
    10561 ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tagcgttgtg
    10621 ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga
    10681 tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca ccgtggtggc
    10741 gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg aacttgaccg
    10801 cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga aagatcagcg
    10861 cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga
    10921 gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc tggcacgctt
    10981 tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac tgtttattcc
    11041 acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg
    11101 tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa tcgtcgggat
    11161 catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg gtcaggaaca
    11221 gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcgtcgg ttgatcagtt
    11281 ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat
    11341 tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa
    11401 aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg gtcgtctgca
    11461 tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga
    11521 cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc
    11581 gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca tcctggacta
    11641 ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg aagagtacaa
    11701 gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca agcgtattgc
    11761 gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta caaaccaaga
    11821 aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc gcatcaggca
    11881 ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac agccttagta
    11941 tccgcaattt cgtagcgggt cacccgtgca ggttttttca aaccttgttt aatctcgaca
    12001 gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc gcctttgagc
    12061 cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc acgcagcgca
    12121 ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca
    12181 gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg aggctgcgta
    12241 aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg aaacggtatt
    12301 catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg cagaacgcct
    12361 gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc actgcaaaat
    12421 caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc aatgcgatct
    12481 catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca ctggtggtat
    12541 tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg ccaccaagga
    12601 tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc cagtagcgga
    12661 acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc ggatcgacca
    12721 gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct tccgaggtgt
    12781 agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg ttgctccaca
    12841 catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga atgcgaatat
    12901 ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc gtgggctgct
    12961 gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa cccataatga
    13021 tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt ccggtatgca
    13081 gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga aattcaagcc
    13141 ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt ttggcattca
    13201 gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc tgtagcgtgc
    13261 agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct ccctccgtat
    13321 ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa cgccaaaaat
    13381 ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa
    13441 accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc ggatctcccc
    13501 tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt cacgcaggct
    13561 ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct tcacgccgcc
    13621 gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac ggtgcaagac
    13681 agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc gcggattcgc
    13741 catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg ttccttccca
    13801 caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga aaattgccga
    13861 agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca gctcattcac
    13921 cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg catcaccatc
    13981 cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca gcgagccatc
    14041 tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag
    14101 ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta catccgccat
    14161 atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt cggcaatcag
    14221 gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg gcaggccaag
    14281 gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat acagcgtttt
    14341 catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc ccgcacctgg
    14401 gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta cgcggtcgaa
    14461 ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg agagtttctg
    14521 gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac gcgccaccgt
    14581 tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg cactgcactg
    14641 cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc gttttggagc
    14701 ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg cgagcaatta
    14761 cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag cgtgacggat
    14821 ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc ccagccctgc
    14881 tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct gcgccagcgg
    14941 gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg cgtctacgat
    15001 gtcgccaatc acattgccgg taaatttacc gtccatgatc tccagttcat tggctaccac
    15061 ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc cgccggaggc
    15121 aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta agcctggcat
    15181 cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc
    15241 cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc gttcggttac
    15301 ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac attcaatctg
    15361 gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt gcgggatttt
    15421 ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc gtgcggtgag
    15481 tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa caatcaccat
    15541 cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc cacgaccata
    15601 cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat caccacttaa
    15661 tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc aggtaatgtt
    15721 aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg aggctacctt
    15781 gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa gatggctcgc
    15841 acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct tgccttgtta
    15901 gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca gcgtaatcca
    15961 cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt tgcaccgctg
    16021 atgcgtaccg actcaccaga tgaaaaacgc attcaggcga tcctcgatca gttaacggat
    16081 gaaagccgca tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat cgcccgttca
    16141 ggcgaaagcg tcgaagtgcg cgaccggcta gcgctcgacg gtaaaaaagc aggcggctat
    16201 tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg ctatctgcgc
    16261 ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa caccactaac
    16321 attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac ccgcacgctg
    16381 ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg
    16441 gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc
    16501 gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt taacctggcg
    16561 gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt tctctggcgc
    16621 aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg
    16681 cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg cgcggtgttc
    16741 cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta cgataaaacc
    16801 atctctacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc cgaagcgtcc
    16861 ggacgtaacg atctggtggt gaaaacagtc gaaggcgacc gcaaagtctc aggctcggcc
    16921 tatcgcgaaa ccaaagatcg tggcttccac cacggcactt tgctgctcaa tgccgacctt
    16981 agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa aggcattacc
    17041 tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac ccatgagcag
    17101 gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt agaagcggaa
    17161 atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt tgcccgtcag
    17221 agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct ggatgaacgc
    17281 tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat cacccgcgcc
    17341 caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa
    17401 ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc
    17461 ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa
    17521 ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc aggagcggga
    17581 gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc gtatcaggca
    17641 taaattatca cttgctgaag taagtaaaat gttaggtatc tcgattaatt ctctaaaata
    17701 gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatgggag aaatttatgg
    17761 ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata aatgaataga
    17821 ttcgtgaata gatacttgaa tgtattttat tttttatcca tatgaaaaat aatagtaaat
    17881 ctataaatca gttgctattg atgaatagat actaaaaagc cggagcagtc ccccactccg
    17941 gcttaacacg acaatcgcct ttcagcgaaa cacaattact ctttatcgcc cagcagaacg
    18001 gattccagtg cgattttgat catgtcgttg aaggtggtct gacgctcagc ggcagtggtc
    18061 tgctcgtgag tgcggatgtg gtcagacaca gtgcagatgg tcagggcttt cgcgccaaat
    18121 tctgcagcga cgccgtagat accagccgct tccatttcca cgccgaggat gccgtatttt
    18181 tccatcacgt cgaacatttc gccgtccgga gagtagaaca ggtcagcgga gaacaggtta
    18241 ccaacgcgag catcaatgcc cagtgcttta gccgcgtcta ccgcgttacg caccatgtca
    18301 aagtcagcga tagcggcaaa gtcatgatct ttaaaacgga tgcggttaac tttggaatcg
    18361 gtgcaggcac ccataccgat aacgacgtcg cgcagtttta cgtgcggcag aactgcgcca
    18421 caggaaccca cgcggataat tttcttcacg ccgaaatcgg tgatcagttc tttggtgtag
    18481 atggagcagg acgggatacc cataccgtga cccattacgg aaattttgcg gcctttgtaa
    18541 gtaccggtga agcccagcat accgcgaacg ttgttcactt cacgggcatc ttcaaggaaa
    18601 gtttcagcaa tatacttcgc acgcagcggg tcgcctggca tcaaaactac gtcagcgaaa
    18661 tcgcccattt ctgcattaat gtgtggggta gccattgttt tatccttatc aacatgtttt
    18721 caccctctcc ctgagggaga gggccggggt gagggcatca agccgcaccc attatcagaa
    18781 catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag tctggccgat
    18841 atccgcgaag gtttcacggt gacccagtga gcccggtttt actttcgggc cgtaaaccag
    18901 taccggaatg tgttcacgcg tgtggtcagt accggtccag gtcggatcac aaccgtggtc
    18961 agcagtgagg atcaggatgt cgtcatcgcg cagcagagac atcagctccg gcagacggcg
    19021 gtcgaacagc tccagaccca cagcataacc ggcgacgtcg cgacggtggc cccaggaaga
    19081 gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt tcatctcttt
    19141 gatggtggcg tcaaacagcg cgtccaggcc agttgctttc actttcttgg tgataccgca
    19201 gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tgaccgtgtt tttcatcaac
    19261 cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aggtcgtgac ggttaccggt
    19321 acgctggaag ttaccggctt tgtcaccgat aaacggacga gcgataacac gaccgatgtt
    19381 gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga gtttatccag
    19441 gccaaaggtt tcttcgtggc aggcaatctg gaacacagag tcagcggagg tatagaaaat
    19501 cggcttgccg gttttcatgt gctcttcgcc cagctgatcc agaatgaccg taccggaaga
    19561 gtggcagtta ccgaggtaac ccggcagatt agcgcgttca accagtttat ccagcagctc
    19621 ttgcgggaag ctgttttcgt gatcggagaa atatccccac tcaaatagaa ccgggacacc
    19681 ggctatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt gcgcccatgc
    19741 gtacgcgccg ataacttcag cgttgccgtc cattcccgcc ggaatgaaac cggtagaacc
    19801 ttcgtgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg gacctttacg
    19861 accgttatca gcttcgcctt tggcacaagc ttctgcgata tgacccaggg tgtcagcccc
    19921 gacgtcacca aagcgttctg catcttctgt agcgccgatg ccgaatgagt ccagcaccat
    19981 aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa ttatcagatc
    20041 agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg gtgctttatc
    20101 ggcaagttta attgccgctt tcaccgcttt cgccgcttcc tgccagctgt tttcgtcttt
    20161 cgcgtggata accgccagcg gacgctgacc gtctacctgg tcgcccaaac gcgccatatc
    20221 agtaaagccg acgctgtaat caatggtgtc agatgcctga cgacgaccgc cgcccattgc
    20281 aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt cggtatcagc
    20341 atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct caacgaagtc
    20401 ggttgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt taccgttgtc
    20461 cagcactgct tgcaatttcg cgcgcgcttc ggcgtcatct ttcgccagtt tgccggagat
    20521 aagcatctcc acgcacagcg ccatcgtgac atcaaacaga cgcgggttac gatactcacc
    20581 cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg aggccagtac
    20641 ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag ccacgccaac
    20701 aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc cgctacccac
    20761 tttcacgtcc atcaccagcg cgtccagacc ttccgcaagt ttcttcgcca gaatagaggc
    20821 ggtgatcagc gggatggagt ccacggttgc ggtaatatca cgggtcgcgt agaaacgttt
    20881 atccgccgga gccagtgagc tggtctgacc gataatcgcc acgccgacgt ctttaataat
    20941 ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt ccagtttgtc
    21001 gagcgtaccg ccagtatgac cgaggccgcg accagagatc atcggaatat agccgccgca
    21061 ggctgcgacc atcggcccca acatcagcga agtcacatcg ccgacgccac cagtggaatg
    21121 tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg ttcctgaatc
    21181 tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga agaaaatggt
    21241 catcgcgagg gcggcaatct gcccttcgga gatagtgttg tcgcgaatgc cgttgataaa
    21301 gaaacgaatt tcttcatcgc tcagtgcatg accatcacgt tttttacgaa taatttcttg
    21361 tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg ccggatgcgt
    21421 ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc gcagcgcatc
    21481 aggcgtaaag catcttactt agtagctgct ggcgctctta ccgtcaccgt ggcccagcgc
    21541 tttcaacagg cttgccagca ggctggaagc accaaagcgg tagtgacgcg catctgccca
    21601 gtcagcaccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt ccgcagtacg
    21661 cacgccgccc gctggtttga aaccaacggt tttttctacg cccatatcac ggatcacttc
    21721 catcatgatg cgcgcgcttt ccggcgtcgc gttcacagcc actttaccgg tagaggtttt
    21781 gatgaagtcc gcacccgctt tgatggagat ttcagacgct ttacggatca gcgcttcgtc
    21841 tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg cgcaagcctc
    21901 tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cccgccatca gcgcgcggta
    21961 cgggaacaca acgtcaactt catcagcacc gtaggcgatt gccgcacggg tttctgccag
    22021 cgcgatttcg atgtcatcgt taccgtgtgg gaagttggtt accgtagcga ttcggatttc
    22081 cggggtgccc tgctctttca gcgttttgcg agcaatcggg ataaagcgag gatagataca
    22141 gatagcggcg gtattgccga ccggggtttt ggcctgatga cacagagcaa ttactttctc
    22201 gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac gcaggctgct
    22261 tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc accttgcgag
    22321 tttgttagta ttctaacatc tactccgcaa cacacttcga tatacatcac aattaaggaa
    22381 atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc actttcaaat
    22441 gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa acttcttaca
    22501 atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat gaaaatgcac
    22561 tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag cagaagcgcc
    22621 attttctggc actggaagca gaaaacaatc tgccttatcc gcagcttcct gccgaagccc
    22681 gccgcgcgct ggatgagggt gtaatctgcg atatgtttga aggtcatgcg ccgtacaaac
    22741 cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa cggttccgaa tggctggagc
    22801 tggaaggcgc gaaagatctt gatgacgcac tctctctgct gaccattctt taccaccacg
    22861 taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg ttgcaaccat
    22921 atgttagaat tctaacacaa gacgagatcg atgttcgaat aaaacgtttc tggcgttacc
    22981 tcgacagaac cctgccagac gcctttatgc acgccaatat cggcccgtct gattcgccca
    23041 ttacccgcgc gatcttacgt gcagacgcag agttgaagca ggtttcaccg aacctgacct
    23101 ttatctacga tcctgaaatc acccctgatg acctgctgct ggaagtggcg aagaacatct
    23161 gtgaatgtag caaaccgcac atcgccaacg gtccggtgca tgataaaatt ttcacaaaag
    23221 ggggctacgg gattgtgagc tgttacaact cactgccgct ggcgggtggt ggcagcacgc
    23281 tggtacgcct taacctgaaa gccattgccg agcgcagcga atcgctggat gacttcttta
    23341 cgcgcactct accgcactac tgccagcagc agatcgccat catcgatgcg cggtgtgaat
    23401 tcctctatca acaatcacac ttctttgaga atagcttcct ggtgaaagaa gggctgatta
    23461 accctgaacg ttttgtgcca atgtttggca tgtatgggct ggcggaagcg gttaacttgc
    23521 tgtgtaagaa agaagggatt gccgcgcgct acggtaaaga agccgccgca aatgaagtgg
    23581 gttatcgcat cagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg aaatatggct
    23641 ggcaaaaacg cgccatgtta cacgcacagt cggggagcag ttccgatatc ggcaccacgc
    23701 cgggcgcgcg tttgccgtat ggtgatgagc cagatccgat cacccatctg caaactgtcg
    23761 ccccgcatca tgcttattat tattccggca tcagcgacat tctgacgctc gacgaaacca
    23821 tcaaacgtaa tccgcaggcg ctggtacagc tttgcctcgg tgcctttaaa gccggaatgc
    23881 gtgaatttac tgccaatgtc agcggtaacg atctggttcg cgttaccggt tatatggtgc
    23941 gtttgtcgga tttagaaaaa tatcgcgccg aaggttcacg caccaacacc acctggctgg
    24001 gcgaagaagc cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg ataagccatg
    24061 aacagcagat gcgctttagt cagtaagatt atccccttct cctgcgttga cgggccaggc
    24121 agtcgtctgg ctctgttttt gcagggctgc aatctgcgct gcaaaaactg tcacaatccg
    24181 tggacgatgg gacgttgcaa tgactgtggg gagtgcgtgc cacagtgtcc gcatcaggcg
    24241 ttgcagattg ttgacggcaa agtggtgtgg aacgctgtgg tttgcgagca gtgtgatacc
    24301 tgcctgaaga ggtgtccgca acatgccacg ccaatggcgc aatccatgag cgtggacgaa
    24361 gtgcttagcc atgtccgcaa agcggtgctg tttattgaag ggataacggt aagcggcggc
    24421 gaagccacga cccaactgcc gtttgtagtg gcgctgttta ctgctatcaa aaacgatccg
    24481 caactgcgcc acctcacctg cctggtggac agtaacggca tgttgagcga aaccggctgg
    24541 gaaaaattac tcccggtgtg tgacggcgca atgctcgatc tcaaagcgtg ggggagcgaa
    24601 tgtcatcaac acttaaccgg acgcgataat cagcagatta agcgcagcat ctgtttgctt
    24661 gcagagcgcg gcaagctggc ggaactgcgt ttgctggtaa ttccagacca ggtggattat
    24721 ttgcatcaca tcgatgagct ggcgacgttt atcaagagac ttggcgatgt tccggttcgc
    24781 ctgaatgcgt ttcatgccca cggcgtgtat ggcgaggcgc aaagctgggc gagcgccacg
    24841 ccggaagacg ttgagccgtt ggctgatgcg ttaaaggtgc gcggggtgag ccggttgata
    24901 tttccggcgc tctatttgtg acatgccgga tacggcctac ggttcggcac agacttgtag
    24961 gcatgataag acgcgacaag cgtcgcatca ggcattatgc gccgactgcc ggatgcggcg
    25021 tgaacgcctt atccggccta cggttcggca cagacttgta ggcatgataa gacgcgtcag
    25081 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    25141 cggttcggca cagact
//
LOCUS       DAZOYL010000025        24660 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZOYL010000025 DAZOYL010000000
VERSION     DAZOYL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24660
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.025"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..702
                     /gene="bcsQ"
                     /locus_tag="ACHUOY_000773"
     CDS             <1..702
                     /gene="bcsQ"
                     /locus_tag="ACHUOY_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR0681636.1"
                     /translation="ITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARA
                     MLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRYQ
                     WILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIGS
                     QVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANWC
                     LLNYSGLKTPVGSAS"
     gene            699..863
                     /locus_tag="ACHUOY_000774"
     CDS             699..863
                     /locus_tag="ACHUOY_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR0681637.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            870..3317
                     /gene="bcsA"
                     /locus_tag="ACHUOY_000775"
                     /pseudo
     CDS             870..3317
                     /gene="bcsA"
                     /locus_tag="ACHUOY_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3328..5667
                     /gene="bcsB"
                     /locus_tag="ACHUOY_000776"
     CDS             3328..5667
                     /gene="bcsB"
                     /locus_tag="ACHUOY_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR0681638.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            5674..6780
                     /gene="bcsZ"
                     /locus_tag="ACHUOY_000777"
     CDS             5674..6780
                     /gene="bcsZ"
                     /locus_tag="ACHUOY_000777"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR0681639.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6762..10254
                     /gene="bcsC"
                     /locus_tag="ACHUOY_000778"
                     /pseudo
     CDS             6762..10254
                     /gene="bcsC"
                     /locus_tag="ACHUOY_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10336..12324
                     /gene="hmsP"
                     /locus_tag="ACHUOY_000779"
     CDS             10336..12324
                     /gene="hmsP"
                     /locus_tag="ACHUOY_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR0681640.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12507..13793
                     /gene="dctA"
                     /locus_tag="ACHUOY_000780"
     CDS             12507..13793
                     /gene="dctA"
                     /locus_tag="ACHUOY_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR0681641.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14013..15509
                     /locus_tag="ACHUOY_000781"
     CDS             14013..15509
                     /locus_tag="ACHUOY_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0681642.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(15605..16534)
                     /gene="kdgK"
                     /locus_tag="ACHUOY_000782"
     CDS             complement(15605..16534)
                     /gene="kdgK"
                     /locus_tag="ACHUOY_000782"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR0681643.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16766..17533
                     /gene="pdeH"
                     /locus_tag="ACHUOY_000783"
     CDS             16766..17533
                     /gene="pdeH"
                     /locus_tag="ACHUOY_000783"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR0681644.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17603..19663
                     /locus_tag="ACHUOY_000784"
     CDS             17603..19663
                     /locus_tag="ACHUOY_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0681645.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19845..21167)
                     /locus_tag="ACHUOY_000785"
     CDS             complement(19845..21167)
                     /locus_tag="ACHUOY_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0681646.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21578..22591)
                     /gene="yhjD"
                     /locus_tag="ACHUOY_000786"
     CDS             complement(21578..22591)
                     /gene="yhjD"
                     /locus_tag="ACHUOY_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR0681647.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22640..23539)
                     /locus_tag="ACHUOY_000787"
     CDS             complement(22640..23539)
                     /locus_tag="ACHUOY_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0681648.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24059..>24660
                     /locus_tag="ACHUOY_000788"
     CDS             24059..>24660
                     /locus_tag="ACHUOY_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR0681649.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5771 a         6279 c         6876 g         5734 t
ORIGIN      
        1 atcaccgccg cattagcctg gtcattacaa atgttgggag aaaatgtcct ggtggtcgat
       61 gcctgcccgg acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc
      121 tgggccagag cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg
      181 cagctcgatt tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac
      241 tggcaaaccc ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt
      301 taccagtgga ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg
      361 agtttgtgcg atcactcgct ggcaatcgtc aatgtggatg ccaactgcca tatccgactg
      421 catcagcaag cgctgccgga tggcgcacat attttgatta atgacttccg tattggcagt
      481 caggttcagg acgatattta ccagctttgg ttgcaaagcc agcgccgatt actgccgatg
      541 ctcattcatc gtgatgaagc gatggctgaa tgcctggcgg ctaagcaacc agtaggtgaa
      601 tatcgcagtg atgcgctggc ggctgaagag atactgacgc tggcgaactg gtgcctgttg
      661 aactactccg ggctgaaaac gccagtcggg agtgcatcat gagtatcctg acccggtggt
      721 tgcttatccc gccggtcaac gcgcggctta tcgggcgtta tcgcgattat cgtcgtcacg
      781 gtgcgtcggc tttcagcgcg acgctcggct gtttctggat gatcctggcc tggattttta
      841 ttccgctgga gcacccgcgc tagcagcgta ttcgcgcaga acataaaaac ctgtatccgc
      901 atatcaacgc ctcgcgtccg cgtccgctgg acccggtccg ttatctcatt caaacatgct
      961 ggttactgat cggtacatcg cgcaaagaaa cgccgaaacc gcgcaggcgg gcattttcag
     1021 gtctgcagaa tattcgtgga cgttaccatc aatagatgaa cgagctgcct gagcgcgtta
     1081 gccataaaac acagcatctt gatgagaaaa aagagctcgg tcatttgagt gccggggcgc
     1141 ggcggttgat cctcggtatc atcgtcacct tctcgctgat tctggcgtta atctgcgtta
     1201 ctcaaccgtt taacccgctg gcgcagttta tcttcctgat gctgctgtgg ggtgtagcgc
     1261 tgatcgtacg gcggatgccg gggcgcttct cggcgctaat gttgattgtg ctgtcgctga
     1321 ccgtttcttg ccgttatatc tggtggcgtt acacctctac gctgaactgg gacgatccgg
     1381 tcagcctggt gtgcgggctt attctgctct tcgctgaaac gtacgcgtgg attgtgctgg
     1441 tgctcggcta cttccaggta gtatggccgc tgaatcgtca gccggtgcca ttgccgaagg
     1501 atatgtcgct gtggccgtca gtggatatct ttgtcccgac ttacaacgaa aatctcaatg
     1561 tggtgaaaaa taccatttac gcctcgctgg gtatcgactg gccgaaagac aaactgaaca
     1621 tctggatcct cgatgacggc ggcagggaag agtttcgcca gtttgcgcaa aacgtggggg
     1681 tgaaatatat cgcccgcacc actcatgaac atgcgaaagc gggcaacatc aacaatgcgc
     1741 tgaaatatgc caaaggcgag ttcgtgtcga ttttcgactg cgaccacgta ccaacgcgat
     1801 cgttcctgca aatgaccatg ggctggttcc tgaaagagaa acagctggcg atgatgcaga
     1861 ctccacacca cttcttctcg ccggacccgt ttgaacgcaa cctgggacgt tttcgtaaaa
     1921 cgccgaacga aggcacgctg ttctatggtc tggtgcagga tggcaacgat atgtgggacg
     1981 ccactttctt ctgcggttcc tatgcggtga ttcgtcgtaa gccgctggat gaaattggcg
     2041 gcattgctgt cgaaaccgtg actgaagatg cgcatacttc tctgcgtttg caccgtcgtg
     2101 gctatacctc cgcgtatatg cgtattccgc aggcggcggg gctggcgacc gaaagtctgt
     2161 cggcgcatat cggtcagcgt attcgctggg cgcgcgggat ggtacaaatc ttccgtctcg
     2221 ataacccgct caccggtaaa ggactgaagt ttgctcagcg gctgtgctac gtcaacgcca
     2281 tgttccactt cttgtcgggc attccacggc tgatcttcct gactgcgccg ctggcgttcc
     2341 tgctgcttca tgcctacatc atctatgcgc cagcgttgat gatcgcccta ttcgtgctgc
     2401 cgcatatgat ccatgccagc ctgaccaact cgaagatcca gggcaaatat cgccactctt
     2461 tttggagtga aatctacgaa acggtgctgg cgtggtatat cgcaccaccg acgctggtgg
     2521 cgctgattaa cccgcacaaa ggcaaattta acgtcaccgc caaaggtgga ctggtggaag
     2581 aagagtacgt cgactgggtg atctcgcggc cctacatctt ccttgttcta ctcaacctgg
     2641 tgggcgttgc ggtaggcatc tggcgctact tctatggccc gccaaccgag atgctcaccg
     2701 tggtcgtcag tatggtgtgg gtgttctaca acctgattgt tcttggcggc gcagttgcgg
     2761 tatcggtaga aagcaaacag gtacgccgat cgcaccgcgt ggagatgacg atgcccgcgg
     2821 caattgcccg cgaagatggt cacctcttct cgtgtaccgt tcaggatttc tccgacggtg
     2881 gtttggggat caagatcaac ggtcaggcgc agattctgga agggcagaaa gtgaatctgt
     2941 tgcttaaacg cggtcagcag gaatacgtct tcccgaccca ggtggcgcgc gtgatgggta
     3001 atgaagttgg gctgaaatta atgccgctca ccacccagca acatatcgat tttgtgcagt
     3061 gtacgtttgc ccgtgcggat acatgggcgc tctggcagga cagctacccg gaagataagc
     3121 cgctggaaag tctgctggat attctgaagc tcggcttccg tggctaccgc catctggcgg
     3181 agtttgcgcc ttcttcggtg aagggcatat tccgtgtgct gacttctctg gtttcctggg
     3241 ttgtatcgtt tattccgcgc cacccggagc ggagcgaaac ggcacaacca tcggatcagg
     3301 ctttggctca acaatgatga taacgcgatg aaaagaaaac tattctggat ttgtgcagtg
     3361 gctatgagga tgagtgcgtt cccctctttc atgacgcagg cgacgccagc aacgcaacca
     3421 ctgatcaatg ctgagccagc tgtagccgcc cagacggaac aaaatccgca ggtggggcaa
     3481 gtgatgccgg gcgtgcaggg cgctgatgcg ccagtcgtgg cgcagaacgg tccttcgcgt
     3541 gatgtgaagc tgacctttgc gcaaattgcg ccgccgccgg gcagcatggt gctacgtggc
     3601 attaacccga acggcagcat tgagtttggt atgcgcagcg atgaagtggt gacgaaggcg
     3661 atgctcaacc tcgaatacac gccatcgcca tcgttactgc ctgtccagtc gcagttaaag
     3721 gtttatctca atgatgagct gatgggcgtg ctgccagtga ccaaagaaca gttgggtaaa
     3781 aaaacgctgg cgcaaatgcc cattaaccca ctgtttatta ccgacttcaa ccgtgtgcgg
     3841 ctggagtttg tcggccatta tcaggacgtg tgcgaaaacc cggccagcac cacgctttgg
     3901 ctggatgttg ggcggagcag tggactggat ctgacctatc agaccctgaa tgtgaagagt
     3961 gacctgtcac acttcccggt gccattcttt gacccgcgcg ataaccgtac taacaccttg
     4021 ccgatggtct ttgcgggtgc gccggatgtt gggctgcaac aagcatctgc cattgtcgcc
     4081 tcgtggtttg gttcgcgttc tggctggcgt gggcagaact tcccggtgct ctataaccaa
     4141 ttgccggatc gcaatgccat tgtctttgca accaacgaca aacggccgga cttcctgcgc
     4201 gatcatccgg cggtaaaagc cccggtgatt gagatgatta accatccgca gaatccttac
     4261 gtcaaactgc tggtggtgtt tggtcgtgac gacaaagacc tgttgcaggc agcgaaaggt
     4321 atcgctcagg gtaacattct gttccgtggt gaaagcgtgg tagtgaatga agtgaaaccg
     4381 ctgctaccgc gtaagccgta cgatgcgccg aactgggtac gtaccgatcg tccggtcaca
     4441 tttggcgaac tgaaaaccta tgaagaacag ttacaatcca gcggtcttga gccagcagcg
     4501 attaacgttt cgctaaacct gccgccggat ctctacctga tgcgcagtac cggcattgat
     4561 atggatatta attaccgcta caccatgccg ccggtgaaag acagttcgcg gatggatatc
     4621 agcctgaata accagttcct gcaatccttc aacctgagca gtaaacagga ggcgaaccgc
     4681 ctgctgctgc ggattccggt attacaaggt ttgctggatg gcaaaacgga tgtctctatt
     4741 ccggcgctga aactgggcgc gaccaaccag ctgcgcttcg actttgagta tatgaacccg
     4801 atgccgggcg gttcggtgga taactgtatt accttccagc cggtgcagaa tcatgtggtg
     4861 attggtgacg attccaccat cgacttctcg aagtattacc acttcatccc gatgccggat
     4921 ctacgcgcct ttgctaacgc gggcttcccg ttcagccgga tggcggatct gtcgcaaacc
     4981 atcaccgtga tgccgaaaac gcctaacgaa gcacagatgg aaacgttgct gaatactgtt
     5041 ggttttatcg gcgcacagac gggcttcccg gcgattaatc tgacggtgac cgatgatggc
     5101 agcaccattc agggcaaaga tgccgacatc atgatcatcg gtggtatccc ggacaaatta
     5161 aaagacgata agcagatcga cctgttggtg caggcgaccg aaagctgggt gaaaacaccg
     5221 atgcgccaga ccccgttccc cggcattgtg ccggacgaga gcgatcgtgc ggcagaaacc
     5281 cagtcaacgc tgacctcttc cggtgcgatg gcggcggtga ttggcttcca gtcgccgtat
     5341 aacgaccagc gcagcgtgat tgcgctgctg gcagatagcc cacgcggtta tgaaatgctt
     5401 aacgatgcgg tgaacgatag cggcaaacgc gccaccatgt tcggttcggt cgcggtgatc
     5461 cgcgagtccg gtatcaacag cctacgtgtt ggcgacgttt attacgtagg tcatctgccg
     5521 tggttcgagc gcttgtggta tgcgctggca aaccatccga ttctgctggc ggtgctggcg
     5581 gcaatcagtg tgatattgct ggcatgggta ctgtggcgtc tgctgcgaat tattagtcgt
     5641 cgtcgtctta acccggataa cgagtaattg aagatgaatg tgttgcgtag tggaatcgtg
     5701 acgatgctgc tgttggctgc ctttagtgtt caggcagcct gtacctggcc tgcctgggag
     5761 cagtttaaaa aggattacat cagtcaggaa gggcgcgtca tcgcccccag cgacgcgcgc
     5821 aaaatcacca cctccgaagg gcaaagttac ggtatgttct ttgccctggc ggctaacgac
     5881 cgtgtagctt tcgataatat tctcgactgg acgcagaaca atctcgctca gggctcttta
     5941 aaagaacgtt tgcccgcctg gctgtggggc aagaaagaga acagtaagtg ggaagtgctg
     6001 gacagcaatt cggcctccga tggtgatgtc tggatggcct ggtcgttgct ggaggcgggg
     6061 cgtttgtgga aagagcagcg ttataccgac atcggcagcg cgttgctaaa acgtatcgcg
     6121 cgggaggaag tggtgacggt gcctgggctg ggttccatgt tgttaccggg caaagtgggt
     6181 tttgctgagg ataacagctg gcgttttaac cccagctacc tgccgccgac gctggcgcag
     6241 tatttcaccc gctttggcgc gccgtggacc acgctgcgcg aaaccaatca acgtttattg
     6301 ctggaaaccg ccccgaaagg tttttcgcca gactgggtgc gctatgagaa agacaaaggc
     6361 tggcagctaa aagccgaaaa aacattgatc agcagctacg acgctatccg cgtttacatg
     6421 tgggtaggca tgatgcctga cagcgatccg caaaaagcgc ggatgctcaa ccggtttaaa
     6481 ccgatggcga cattcactga gaaaaacggt tatccgccgg aaaaagtgga tgtggctacg
     6541 gggaaagcgc agggtaaagg accgatcggt ttttctgccg ccatgctgcc ctttttacaa
     6601 aaccgcgatg cgcaggccgt tcagcgccag cgcgtggccg ataactttcc cggcagcgat
     6661 gcctattaca actatgtgct gaccctgttt ggacaaggct gggatcaaca ccgtttccgc
     6721 ttctcgacaa aaggtgagtt attacctgac tggggccagg aatgcgcaaa ttcacactaa
     6781 acatattcac gctttccctc ggtctggccg tcatgccgat ggtcgaggca gcaccaaccg
     6841 ctcagcaaca gttgctggag caagttcggt taggcgaagc gacccatcgt gaagatctgg
     6901 tgcaacagtc gttatatcgg ctggaactta ttgatccgaa taacccggac gtcgttgccg
     6961 cccgtttccg ttctttgtta cgtcagggcg atattgatgg cgcgcaaaaa cagctcgatc
     7021 ggctgtcgca gttagcgccg agttcaaatg cgtataaatc gtcgcggact acgatgctac
     7081 tttccacgcc ggatggtcgt caggcactgc aacaggcacg attgcaggcg acgaccggtc
     7141 atgcagaaga agctgtggcg agttacaaca aactattcaa cggtgcgccg ccggaaggtg
     7201 acattgctgt cgagtactgg agtacggtgg cgaaaattcc ggctcgccgt ggcgaagcga
     7261 ttaatcagtt aaaacgcatc aatgcggatg caccgggcaa tacgggcctg caaaacaatc
     7321 tggcgctatt gctgtttagt agcgatcgcc gtgacgaagg ttttgccgtc ctggaacaga
     7381 tggcaaaatc gaacgccggg cgcgaagggg cctctaaaat ctggtacggg cagattaaag
     7441 acatgcccgt cagtgatgcc agtgtgtcgg cgctgaaaaa atatctctcg atctttagtg
     7501 atggcgatag cgtggcggct gcgcaatcgc aactggcaga acagcaaaaa cagctggccg
     7561 atcctgcttt ccgcgctcgt gcgcaaggtt tagcggcggt ggactctggt atggcgggta
     7621 aagccattcc cgaactacaa caggcggtgc gggcgaaccc gaaagacagt gaagctctgg
     7681 gggcgctggg ccaggcgtat tctcagaaag gcgatcgcgc caatgcagtg gcgaatctgg
     7741 aaaaagccct cgcactggac ccgcacagca gcaacaacga caaatggaac agtctgctga
     7801 aagtaaaccg ctactggctg gcgatccagc agggcgatgc tgcgctgaaa gccaataatc
     7861 ctgaccgggc agaacgcctg ttccagcagg cgcgtaatgt cgataacacc gacagttatg
     7921 cagtgctggg gctgggcgat gtggcgatgg cgcgaaaaga ttatcccgcc gccgaacgtt
     7981 attatcagca gaccttgcgt atggacagcg gcaacactaa cgccgtgcgc gggctggcaa
     8041 atatttaccg ccagcaatcg ccagaaaaag ctgaagcgtt tatcgcctcg ctctctgcca
     8101 gtcagcggcg tagcattgat gatatcgaac gcagcctgca aaacgaccgt ctggcacagc
     8161 aggcagaggc actggaaaac cagggcaaat gggcgcaggc ggcagcactt cagcggcaac
     8221 gactggcgct ggaccccggc agcgtatgga ttacttaccg actttcgcag gatctctggc
     8281 aggccggaca acgcagccag gccgatacgt taatgcgcaa tctggcgcag cagaagtcga
     8341 acgacccaga gcaggtttac gcttacgggc tgtacctacg cttacgggct gtacctctct
     8401 ggtcatgacc aggacagagc ggcgctggcg catatcaaca gcctgccgcg tgcgcagtgg
     8461 aacagcaata ttcaggagct ggttaatcga ctgcaaagcg atcaggtgct ggaaaccgct
     8521 aaccgcctgc gagaaagcgg caaagaggca gaagcggaag cgatgctgcg ccagcaacca
     8581 ccttccacgc gtattgacct cacgctggct gactgggcgc aacaacgacg tgattacacc
     8641 gccgcccgcg ctgcatatca gaatgtcctg acgcgggagc cagctaacgc cgacgccatt
     8701 cttggtctga cggaagtgga tattgctgcc ggtgacaaag cggcggcacg tagccagctg
     8761 gcgaaactgc ccgctaccga taacgcctcg ctgaacacac agcggcgcgt ggcgctggca
     8821 caggcgcagc ttggcgatac cgcagcagcg cagcggacgt ttaataagtt gatcccgcag
     8881 gcaaaatctc agccaccgtc gatggaaagc gcgatggtgc tgcgtgatgg tgcgaagttt
     8941 gaagcgcagg cgggcgatcc aacgcaggcg ctggaaacct acaaagacgc catggtcgca
     9001 tccggtgtga ctacgacgcg tccgcaggat aacgacacct ttacccgact gacccgtaac
     9061 gacgagaaag atgactggct gaaacgtggc gtgcgcagcg atgcggcgga cctctatcgc
     9121 cagcaggatc ttaacgtcac ccttgagcac gattactggg gttcgagcgg caccggtggt
     9181 tactccgatc tgaaagcgca cactaccatg ttgcaggtgg atgcgccgta ttctgacggg
     9241 cggatgttct ttcgcagtga tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc
     9301 gatggcaaat gggatgacaa ctggggcacc tgtacattac aggactgtag cggcaaccgc
     9361 agccagtcgg attccggtgc cagcgtggcg gtcggctggc gaaatgacgt ctggagctgg
     9421 gatatcggta ccacgccgat gggcttcaac gtggtggatg tggtcggcgg catcagttac
     9481 agcgatgata tcgggccgct gggttacacc gttaacgccc accgtcggcc catctccagt
     9541 tctttgctgg cctttggtgg gcaaaaagac tccccgagca ataccgggaa aaaatggggt
     9601 ggcgtacgtg ccgacggtgt ggggctaagt ctgagctacg ataaaggtga agcaaacggc
     9661 gtctgggcat cgcttagtgg cgaccagtta accggtaaaa atgtcgaaga taactggcgc
     9721 gtgcgctgga tgacgggcta ttactataag gtcattaacc agaacaatcg ccgcgtcaca
     9781 atcggcctga acaacatgat ctggcattac gacaaagatc tgagtggcta ctcactcggt
     9841 cagggcggtt actacagtcc gcaggaatac ctgtcgtttg ccataccggt gatgtggcgg
     9901 gagcgcacgg aaaactggtc gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc
     9961 accaaaacca tgccgcgtta tccgctgatg aatctgatcc cgaccgactg gcaggaagaa
    10021 gctgcgcggc aatccaacga tggcggcagc agtcagggct tcggctacac ggcgcgggca
    10081 ttacttgaac gacgtgttac ttccaactgg tttgttggca cggcaattga tatccagcag
    10141 gcgaaagatt acgcacccag ccatttcctg ctctacgtac gttattccgc cgccggatgg
    10201 cagggtgaca tggatttacc gccgcagccg ctgatacctt acgccgactg gtaagttttc
    10261 agatagcgcc tctcttaatg ccgctgcgat cgggtatact cgggcggcaa tctgggattt
    10321 ccggggggag acaatttgcg cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg
    10381 gcagccgttg tcctggtgtt cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc
    10441 cagcagaatc gctataacac ggctacgcaa ctggaaagca ttgctcgctc tgtccgcgaa
    10501 cccttatctt cagctatttt gaaaggcgat attcccgaag cggaagctat tcttgccagc
    10561 attaaaccgg caggcgtggt cagccgtgcc gatgtagtgc tgcctaacca gttccaggcg
    10621 ctgcgtaaaa gttttattcc agagcgcccg gtgccgataa tggttactcg cctgtttgag
    10681 ctaccggttc aaatctcgct gggcgtttac tcgctcgaac gtccggcaaa cccgcagcca
    10741 attgcctatc tggtactaca ggcggattcc ttccgtatgt ataagttcgt gatgagcacc
    10801 ctctcaacgt tagtgaccat ttacttactt ttgtcgctta tcctgaccgt cgccatcagc
    10861 tggtgcatta accgcctgat tttgcatccg ttacgcaata ttgctcgcga acttaacgcc
    10921 atcccagccc aggagcttgt tggtcaccaa ctggcattac cgcgtctgca tcaggacgat
    10981 gaaatcggta tgttggtgcg cagttacaac ctcaaccagc aattgctgca gcgccattat
    11041 gaagaacaga acgaaaatgc gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg
    11101 ctgatggaga tgctggagca ggttgtcgcg cgtaaacaaa ccaccgcgct gatgatcatc
    11161 acctgtgaaa ccctgcgtga tactgcgggc gtgctgaaag aggcgcaacg agaaattctg
    11221 ctgctgacgc tggtggaaaa actcaaatcg gtactgtcgc cacgtatgat cctcgcgcag
    11281 attagcggtt atgactttgc tgtcattgcc aacggtgtac aggaaccgtg gcacgcaatc
    11341 accttaggtc agcaagtgct caccatcatg agcgagcgcc tgccgattga acgtattcaa
    11401 ctccgtccgc actgtagcat tggcgtggcg atgttctacg gcgatctcac cgccgaacag
    11461 ctttacagtc gcgctatttc tgcggcattt accgctcgcc ataaaggcaa gaatcagatt
    11521 cagttctttg atccgcagca gatggaagcc gcccagaagc ggttgacgga agagagcgat
    11581 atccttaatg cactggaaaa tcatcagttt gctatttggt tacagccaca ggtcgagatg
    11641 accagcggta aactggtcag tgcggaagtg ttactgcgta tccagcaacc ggatggcagt
    11701 tgggacctgc cggatggctt aatcgatcgc attgagtgct gtgggctgat ggttaccgtc
    11761 ggtcactggg tgctggaaga gtcctgtcga ttgcttgcag cctggcaaga gcgcggcatt
    11821 atgctgccct tgtcggtaaa cctctctgcg ctgcaactga tgcacccaaa tatggtggcg
    11881 gatatgctgg aactgttaac ccgctatcgc attcagccgg gaacactgat tctggaagtg
    11941 acagaaagcc gacgtattga cgaccctcat gctgcggtgg caatcctccg tccgctgcgc
    12001 aatgccggag ttcgggtggc gctggatgat ttcggcatgg gctacgcagg gctgcgtcag
    12061 ctgcagcata tgaaatcgtt gccaatcgac gtactgaaaa tcgacaaaat gtttgttgaa
    12121 ggcttgccgg aagatagcag catgattgct gcaattatca tgctggcgca gagcctgaac
    12181 ttacaaatga ttgccgaagg cgtggagact gaagcacaac gcgactggct ggcaaaagcg
    12241 ggcgttggta ttgcccaggg cttccttttt gctcacccac tccctattga aatcttcgaa
    12301 gagagttacc tggaagaaaa gtagctaccc caaactgatt acaaaacttt aaaaagtgct
    12361 ggtttgtgcg agccagctca aactttttaa cctttttgtt tcaattatga tccaggtaca
    12421 tttctgtgat gttgtctggg tgttatttta aggccgcagg taccccataa ccttacaaga
    12481 cctgtggttt tactaaagga caccctatga aaacctctct gtttaaaagc ctttactttc
    12541 aggtcctgac agcgatagcc attggtattc tccttggcca tttctatcct gaaataggcg
    12601 agcaaatgaa accgcttggc gacggcttcg ttaagctcat taagatgatc atcgctcctg
    12661 tcatcttttg taccgtcgta acgggcattg cgggcatgga aagcatgaag gcggtcggtc
    12721 gtaccggcgc agtcgcactg ctttactttg aaattgtcag taccatcgcg ctgattattg
    12781 gtcttatcat cgttaacgtc gtgcagcctg gtgccggaat gaacgtcgat ccggcaacgc
    12841 ttgatgcgaa agcggtagcg gtttacgccg atcaggcgaa agaccagggc attgtcgcct
    12901 tcattatgga tgtcatcccg gcgagcgtca ttggcgcatt tgccagcggt aacattctgc
    12961 aggtgctgct gtttgccgta ctgtttggtt ttgcgctcca ccgtctgggc agcaaaggcc
    13021 aactgatttt taacgtcatc gaaagtttct cgcaggtcat cttcggcatc atcaatatga
    13081 tcatgcgtct ggcacctatt ggtgcgttcg gggcaatggc gtttaccatc ggtaaatacg
    13141 gcgtcggcac actggtgcaa ctggggcagc tgattatctg tttctatatt acctgtatcc
    13201 tgtttgtggt gctggtattg ggttcaatcg ctaaagcgac tggtttcagt atcttcaaat
    13261 ttatccgcta catccgtgaa gaactgctga ttgtactggg gacttcatct tccgagtcgg
    13321 cgctgccgcg tatgctcgac aagatggaga aactcggctg ccgcaaatcg gtggtggggc
    13381 tggtcatccc gacaggctac tcgtttaacc ttgatggcac atcgatatac ctgacaatgg
    13441 cggcggtgtt tatcgcccag gccactaaca gtcagatgga tatcgtccac caaatcacgc
    13501 tgttaatcgt gttgctgctt tcttctaaag gggcggcagg ggtaacgggt agtggcttta
    13561 tcgtgctggc ggcgacgctc tctgcggtgg gccatttgcc ggtagcgggt ctggcgctga
    13621 tcctcggtat cgaccgcttt atgtcagaag ctcgtgcgct gactaacctg gtcggtaacg
    13681 gcgtagcgac cattgtcgtt gctaagtggg tgaaagaact ggaccacaaa aaactggacg
    13741 atgtgctgaa taatcgtgcg ccggatggca aaacgcacga attatcctct taatctcacc
    13801 acttatgccc gcactccctt ccccgagtgc gggtgtttgc gcataattga cccctgtgcc
    13861 tgaacttacc acctgggtga gttcaggttt atttcacttc ttccgtgaca ttttcatgtt
    13921 cttgcggtct aacacgaagt gtttttaacg tcatttagac tgactgacaa cgggcagttt
    13981 tttattacca ggatagttga tcaggggttc acatgcaggg cacaaaaatt cgacttttag
    14041 cgggcggttt gctgatgatg gccactgctg gctatgtgca ggcagatgcg ctccagcctg
    14101 atccagcatg gcaacagggg acgctttcca acggtttaca gtggcaagtg ctgaccaccc
    14161 cccagcgtcc cagcgatcgt gttgaaattc gcctgctggt taatatcggt tcgctcgccg
    14221 aaagtacaca acagagcggt tacagtcacg ccatccctcg tattgcgcta acgcaaagcg
    14281 gtggccttga cgcagcacag gcgcgttcat tgtggcagca ggggatcgac cctaaacgcc
    14341 cgatgccgcc ggtaattgtc tcttatgaca ccacgctgtt taatctgagt ttgcccaata
    14401 accgtaacga tttgctgaaa gaagcgctct cttatctggc aaatgccact ggcaaattga
    14461 ccatcacacc agaaaccatc aaccacgcgc tgcaaagtca ggacatggtg gcaacctggc
    14521 ctgccgatac taaagagggc tggtggcgct atcgtctgaa agggtcaacc ttgttaggtc
    14581 acgatcctgc cgatccgctg aaacaacccg ttgaagcgga aaaaattaaa gatttctatc
    14641 agaaatggta caccccggat gcaatgacgc tactggtggt gggaaacgtg gatgcgcgct
    14701 cggttgtcga ccaaatcaac aaaacgtttg gcgaactgaa aggcaaacgt gaaacgccag
    14761 ctccggtgcc gacgctttct ccgctgcgtg cggaagcggt gagtattatg actgacgcgg
    14821 tgcgtcagga ccggttatct atcatgtggg atacgccgtg gcagccgatt cgtgaatcag
    14881 ccgcactgct gcgctactgg cgtgcggacc tggcccgcga ggcgctgttc tggcatgttc
    14941 agcaagcgtt aagcgccagt aacagcaaag acatcggtct tggatttgac tgccgtgtgc
    15001 tgtatctgcg tgcgcagtgt gccatcaaca tcgaatcacc aaacgacaag ctgaacagca
    15061 accttaatct ggtggcgcgt gaactggcga aggttcgcga taaaggtctg ccggaagaag
    15121 agttcaatgc gttagtggcg caaaagaaac tggagctgca gaaactgttt gccgcctatg
    15181 cgcgggctga taccgatatt ctgatgggtc agcggatgcg ttcgctgcaa aatcaggtcg
    15241 tcgatatcgc gccggagcag tatcagaaac tgcggcagga tttccttaac agcctgacgg
    15301 tggagatgtt aaatcaggat ctgcgtcagc agttgtcgaa tgatatggcg ttaatactgc
    15361 tgcagccgaa aggcgagccg gaatttaaca tgaaagcgtt gcaggcggcc tgggatcaaa
    15421 tcatggcccc atcgactgct gctgccgcca cctctgtcgc cacggatgac gtacatcctg
    15481 aagtgacgga tattccacct gcacagtaat gcctgatggt gatgagttag cacaaccatc
    15541 aggtatttat ggatgagctg gatagagggg taacacttta tccagccttt tgcatatgct
    15601 gcgtttacgc tggcatcgcc tcacgcggga taatcgcgcc gcgatactga ataacggtac
    15661 ttgcggtcag gtgcccgcgt ttcgccgcgt tttccgcact gccgcccgtc aaacataccg
    15721 ccagataacc ggcgctgaaa gagtcgccag ctgcggtggt atcgatcact ttttctttcg
    15781 gcagtttcac cgccggaaca tccactaacc cttcgccagc aatggacacc aggcaagaat
    15841 ccgccccgcg tttcaccacc acttctttca cgcccgcgtt atgggtgcgc gcaatgacgt
    15901 cttccaccgg ctgttgaccc cacagcgcgt cttcatcgtc cagcgtcagg aaggcgatat
    15961 ccgtgcattc cagcatttgt tggtacacct gctgtgtctc ttctttgctg gcccacaggc
    16021 gcggacgata gttattgtcg aaaatcactt ttccgccgtt ggcgcggcat tcgcgcagca
    16081 gggaaagcag cttttcgcgg ctggtcgggc ttaagatcgc caggctaatc ccgctcaggt
    16141 agagataatc gaaattcgcc agctcttcgc aaatcgccgc agactgctca ctctccagcc
    16201 agaatttggc ggcggcttcg ttccgccagt agtagaacgt acgctcgccg gtgctgtcgg
    16261 tttcaatgta gtaaaggccc ggcagacggt tttccatccg ttgggtcagg gaagtatcaa
    16321 cgttctcgcc gtgccaggcg tccagcattt gctggctaaa actgtccgtt cccagcgccg
    16381 ttacgtaatg aacggttaat gccgcaggat cgacctgacg ggcgatatag acggaagtgt
    16441 tcagggtatc gccgccgaaa ccgcgcttaa cgtccgcgcc tttctcggaa agctcaatca
    16501 tgcattcgcc aatcacggca atctttttgg acatagtcgt gaacctgatc tgtaaaaaat
    16561 tagcgttagt ttgcgctgtg gtggtttgct ggtcaaccat attaaaacag tgttccatta
    16621 tttttttgag ccagaacgta ttcctgaaag attccgttgt ggagtgagga aatttacccc
    16681 ggccaggcgg taaagttctg cccttgcgcg ccgataatct ttgtcgagtc cgggcagcat
    16741 cacttttaaa cacaggacat ctttgatgat aaggcaggtt atccagcgaa taagcaaccc
    16801 tgaagcaagc atcgagagct tgcaggaacg gcgtttttgg ttgcagtgtg agcgtgctta
    16861 cacctggcag ccgatctatc aaacatgcgg acggttaatg gccgtggagc tattaacggt
    16921 ggtcacgcat cccttgaacc cttcgcaacg cctgccgccg gatcgctatt ttactgaaat
    16981 caccgtcagc catcggatgg aggttgtgaa agagcagatt gatttgctgg cgcaaaaagc
    17041 cgacttcttt gtagagcacg acctgctggc atcggtcaat attgatggcc ctacgctcat
    17101 cgccctgcgt cagcaaccaa aaatcctgcg ccaaattgag cgtcttccct ggctgcgttt
    17161 cgaactggtg gaacacatcc gcctgccgaa agattcaacc tttgcctcga tgtgtgaatt
    17221 tggcccgctg tggctggatg attttggcac cgggatggca aatttctctg cgctaagtga
    17281 agtgcgttat gactacatca aaatcgcgcg agagctgttc gtgatgctgc gtcagtcgcc
    17341 ggaaggccgc acactctttt ctcagctttt acatctaatg aatcgctatt gtcgcggggt
    17401 gattgtcgag ggcgtagaaa caccggaaga gtggcgtgat gttcagaact cgcccgcatt
    17461 cgccgcacaa ggctggtttc tttcacgccc ggcaccgatg gaaacgctga atacggcggt
    17521 tctggcgcta taagctgcct cattttccgc tgggctggac tatctttagg actggcacag
    17581 gaagaatatg aggaagcgaa cgatgagcaa ggcaggcaaa ataaccgctg cgatttcagg
    17641 ggctttcttg ttgttgattg tcgtggcgat cattttgatt gcaacttttg actggaatcg
    17701 actcaaaccg accatcaacc agaaagtctc tgcggagttg aatcgtccgt tcgctatccg
    17761 tggcgatctg ggcgtggtgt gggagcggca aaaacaagaa actggctggc gcagctgggt
    17821 gccgtggcct catgtacatg cagaagacat cattcttggt aatccaccgg atattcccga
    17881 agtcacgatg gtgcatttgc cgcgagtaga ggcaacgctg gcaccgctgg cgctgctgac
    17941 caaaacggtc tggctgccgt ggttcaagct cgaaaagccc gacgcgcgcc tgattcgcct
    18001 ctccgaaaag aacaataact ggacgtttaa tcttgccaac gatgacaaca aagacgcgaa
    18061 tgcaaagccg tcggcatggt cgtttcggct ggataatatt cttttcgatc aagggcggat
    18121 cgccattgat gacaaagtaa gcaaagcgga tctggaaatt tttgttgatc cgttaggcaa
    18181 gccgctgccg ttcagcgaag ttactggatc gaaaggtaaa gcggataaag aaaaggtggg
    18241 cgattacgtt tttggcctga aggcgcaggg acgttataac ggtgaaccgc tcacgggtac
    18301 gggaaaaata ggcggtatgc tggcgctgcg tggcgaaggg acgccgtttc cggtacaggc
    18361 tgatttccgt tcaggtaata cccgtgttgc ttttgatggc gtcgtgaatg acccaatgaa
    18421 gatgggcggt gtcgatttac ggcttaaatt ttctggcgat tcactgggtg atctctatga
    18481 actgacgggc gttctgctgc ccgatacccc gccgtttgaa acggatggtc ggctggtagc
    18541 gaaaatcgac actgaaaaat cgtcggtctt tgattatcgc ggttttaatg ggcgaattgg
    18601 cgatagcgat atccacggtt ctctggtcta caccaccgga aagccacgac caaaactgga
    18661 aggtgatgtc gagtcgcggc aattgcggct ggcggacctg ggaccgttga ttggcgttga
    18721 ttccgggaaa ggggcagaaa agtcgaaacg gtctgaacag aagaagggcg aaaaaagcgt
    18781 tcagcctgcg ggcaaagtgc tgccttatga ccgcttcgaa accgataaat gggacgttat
    18841 ggatgccgat gttcgcttca aagggcggcg cattgagcat ggcagtagcc tgccgattag
    18901 cgatctttct actcatatca tcctcaaaaa tgctgacctg cgcctgcaac cgctgaaatt
    18961 tggcatggcg ggcggcagca ttgcgacgaa tattcatctg gaaggcgata aaaagccgat
    19021 gcaggggcgg gcagatattc aggctcgtcg actgaaactg aaagaactga tgcccgatgt
    19081 ggaactgatg cagaagacgc tgggggaaat gaacggtgac gcggaactac gcggtagcgg
    19141 taactcggtg gcggcgcttt taggcaacag taacggcaac ctgaaactgt tgatgaatga
    19201 cgggctggtg agccgcaacc tgatggagat tgttgggctg aatgtcggca actacattgt
    19261 cggtgcgata tttggtgacg atgaggtgcg ggtgaactgc gcggcggcga atctgaatat
    19321 tgccaacggc gtggcgcgcc cgcagatttt tgctttcgat actgagaacg cgttgattaa
    19381 tgttaccggc acggcaagtt ttgcttcaga acagctggat ttgactattg atccggagag
    19441 taaagggatt cggattatca cactgcgttc gccgctgtat gtgcgtggga cgtttaaaaa
    19501 tccgcaggct ggggtgaaag ccggaccgct gattgcccgt ggtgctgttg ctgcggcact
    19561 ggcaacgctg gtaacaccga cggcggcgtt actggcactg atctcacctt ccgaagggga
    19621 ggctaatcag tgtcggacga tattgtcgca gatgaagaag tgatgataat tgccggatgt
    19681 ctgacatccg gcaattaaac agatttagat ttttggcaaa gtgtgctttg ttcatgccgg
    19741 atgcggcgtg aacgccttat ccggcctaca aatccttgtg aattcaatat attgtatgga
    19801 aattcagtcc tgataagcgt agcgcatcag gcttttcaca cttattacaa cgactgatgt
    19861 cgcgtctcat gggtcagcag cagggcgatt aacgtcaagc cagccatcgc cgccagatat
    19921 aaccccaccg cacctaaccc gtagttagtc tgcaaccagg ctgcgatata cggtgcaacg
    19981 gaagccccga gaatcgacgc tacgttgtaa gagaatgatg ctccggtgta acgcacttct
    20041 gttgggaaca gctctggtaa cagcgcgccc atcggcccga aggtcagccc catcagactt
    20101 aaccccagca gcaggaaggc aaaaaccaga atcgggttgc cagaaccgag cagcgggtta
    20161 aaggcgaaca gcgcgaacag gatgatcagc gtggtgataa ttaccatgct tttacggcga
    20221 ccaaaggcat cagccagtaa tccggcgact ggcaccatca cgccaaaacc aataactgcc
    20281 atcatcagca tccacaacac ttcgttacgc ggcaggccaa gcccaactgg cgcggcggcg
    20341 gtactgaagg tcatagagta gaccgtcatg atgtaaaaca gcgtataggt tgccagcatg
    20401 atgaacgtac ccagtacggt tacgcgaaca tgtttggtca gcagcgtacc cagcgggatc
    20461 ttcacctgtt ttttcgcttt agcgactttc tcaaacaccg gcgactcatg cagcgacacg
    20521 cgaacataca ggccgataat gaccagtacc gccgagaaga taaatggcac acgccagccc
    20581 cagctcataa actgctcgtc ggtcagtagc caggaaagca gcaagaaagt gccgttggca
    20641 aagaagaagc caatcggtgc gcccagctgt gggaaggagc catacagtgc acgtttgcgc
    20701 ggtggggcgt tttcagtcgc cagcagcgcc gcgccgcccc attcaccgcc taagcccaga
    20761 ccctgaccaa atcgagccag cgccagcagc agcggggcga aaataccaat cgtggcatag
    20821 cccggcagca gaccaatcac cacggttgaa atccccatcg ttagcaacga ggcgaccagc
    20881 gtcgctttac gcccaacgcg atcgccaaaa tgaccaaaaa cggcagagcc aatggggcgc
    20941 gcgacgaagg cgatggcgaa ggtggcgagc gactgtagcg ttgctgccgt tggatcgccc
    21001 tgcgggaaga agatatgcgg aaacacaata acggccgcag tggcgtaaat gtaaaagtcg
    21061 aagaactcaa tggccgtgcc aatgagagag gcgacaagga ctttattacg cgagttgatc
    21121 ggcgtgtatt cttgctcgtg gtcgagtgtt gtggctgttg cttgcataat cttttcttat
    21181 tttttggcta acgaatagcc atattacgca cagcaaaagt gatatttcaa tgtgtaacaa
    21241 atcgcggaaa tggtcaaaaa ccaggcaaaa aggggataat gtttaggcca gtgatttttc
    21301 ttctatgaaa tgcttcacat aattttataa atagtggata aaactggttt caggttaaat
    21361 aaaagttacc aactggattt atatgctggg gttatgagtt ggaatgaaat tgtttgctcc
    21421 caaatagatg gctgaagagg ctctggggaa gagtttcaag aggttatgtg cgcattatag
    21481 ccggatgcga tgctggcgca tcttatccgg cctacgggga gccaggccga ataaaatcgt
    21541 atgccggata aggcgtttac gccgcatccg gcctatttta aggctgcgtt ttccctggca
    21601 ttcgcgggtc gtctttatat tcggcggtgg caatccacgc cgcgcaaaac agcgtcaaac
    21661 gggcgaagaa gtagaaaaat gccatcagcc ccagcacgga accaaatgct gcgccagagg
    21721 gggatttcat cagcgatggc agggtgtagg tcatcacgat tttaatcact tcaaaaccaa
    21781 tagcagccag aaatgttccg cgaatcaggg cttttttgcg cggacggtgg cgcggtaacc
    21841 gccagaagat ccagaagaaa agcagatagt tggcgaagat ggaaatcgcc aggccaatca
    21901 accgccacgt cggtttcagc cactcaatgc tgttcaggtg cagggcgcta ataatcattt
    21961 gctgcgccga accggcaacc gaggtgatcg aaagcgtcac aatcagcgca atcaacaaac
    22021 caatcagcga aataaaatca cgtagatatt ttacccagaa cttctcctga tcttgcggcg
    22081 agcgttccca gacatcgcgc gactgggcgc gaatcgcttc acgcaggtta cccatccagt
    22141 tgatgccgga ataaagcgcc acagccaggc cgacaagccc tacagtcgta cgctgctgaa
    22201 cggcggtgtt gatggtgttt ttcaacgtgg cggctagcgt cggatcgctg atgttttgca
    22261 gaattttgtc gaagatatcc tgtagcaaca tcggatggga ggccagcaca aagccccctg
    22321 cggcaaacga caccatcaaa atcgggatca tcgacaaaaa tgagaaatag gtgatagccg
    22381 caccaaactg gttacccagc cgatcgttaa agcgttctgt cgcgcgaatc aggtgcgcaa
    22441 taaccggttg gcgttggact ttttcggcag tggtggtgac ggtttccagc gcctggctaa
    22501 ctttgccgcc tttctcgcta ttatccaact gcttaatcgg ctcgtgctcc agatcctggg
    22561 tgggacgttt gatctcgttt tcctgcgtca tccgctcttt tgtccttctg ttgtggtcat
    22621 tcttaaaaag tatagtcggt cagtccaggt actctttcat tacgccagcc agccactgca
    22681 taaacaggtt tacacgccgg gaaagctccc gacgctgcgg ataaaccagc gaaagggaga
    22741 gcggctcggc acggtagcct ggcaacactt caataagctg cccggcacgc agggcttcgc
    22801 gcacggcaat gcgcgggatc tgaataatcc ccagaccggc aagaccggcg gcgagatagt
    22861 tttcgctact gtttaccgtc aacatgccgc cagacttaaa ccactggacg ccattaacgc
    22921 tggcaacctc aaaacctaac ggatgtacac ccaggtgcgg tgtataacgc accattgcgt
    22981 gtgaagtcag atcatcgggg ctttgcggat aaccaaagcg cgtcagatag tgcggactgg
    23041 cacagttgac catggtcagt ttgccgaggg gacgggcgat aacgccatct tccggtaacg
    23101 cgccagtgcg tatcacgcaa tcaaaaccat catgaagaat atctaccgga cggttatggc
    23161 tactcagttc cagctcaatt cccggatgct gatagagaaa ttccgacaga cgcggcagta
    23221 acaggttttt cgcgattcct gacgggatgt cgatgcgtaa tttaccgctg atactggtgg
    23281 catcctgttg aaacagaccg tccagttcgc tgagattact caacacatct tttgcccgtt
    23341 gataataggt catgccttct ggcgtgagtt tgacccgtcg cgtagtgcgc tggagaagct
    23401 gggtgccaag ttgatgttcc agtgcctgta tctggcgcga aacacttccc tttggcaaag
    23461 caaagaaatc cgccgcgcgg gaaaaacttt ccagctccgc gactttgatg aacaactgca
    23521 ttgcgtgaat tttatccatt tctttgaccg attgttgttc acagcgaaac agtaacgcgc
    23581 atatcgcatt gtttattgtt ttttctaaca tctaatgccg ggtttcacaa tgttgtcatg
    23641 acgaatgtgg cgatttctca gcaggaaaag ggcggaatag ctttatgaga aaggtgaatc
    23701 cccttaaaca gagcgtattt ttcagtgatt ccctttaaag tgaatagagg acgtattggc
    23761 tatttgtgct tatttttgtc tcattgccat ttgttatttt ggcttgtagt cgcggaattt
    23821 catttttaat tgcatgcgta ctaaccacca atataaggaa atcctcaaaa ttgttttaaa
    23881 tagtgccctc gacatgtaat ttcaggcgtt tagctgtaac tatttatatc ggtaactatt
    23941 ttctacgcgt catctctgta gaatggccac cgccaaatgg cgaattctta ttttttctcg
    24001 cgggtaatac ccttccacta cttaagtctt tttaacgtat tcaggtggag attcagttat
    24061 gcaaatagtc atgtttgaca ggcagtcaat atttattcat ggaatgaaaa tcagtttaca
    24121 gcagcgtatt ccaggagtga gtattcaggg ggccagtcag gcagacgagt tatggcaaaa
    24181 gctggaaagt taccctgaag ccttagttat gctcgatggc gatcaggatg gtgagttttg
    24241 ctactggttg ctgcaaaaaa ccgtggtgca gtttcctgag gttaaggtgt taatcacggc
    24301 gacggattgc aataaacggt ggttacagga agttattcat tttaatgtgc tggccattgt
    24361 gcctcgtgat tcaaccgtcg agacatttgc gttggcggta aatagtgcgg cgatggggat
    24421 gatgtttctg cctggagact ggcggacgac gccggaaaag gacattaaag atctcaaatc
    24481 gttaagcgcc cgccagcggg agattttaac catgttagcg gcgggcgaat caaataagga
    24541 gatcggcaga gcgctgaata tcagtaccgg aacggtaaaa gcgcatctgg aatccctgta
    24601 tcgccgtctg gaagtgaaaa atcgcaccca ggcggcaatg atgttaaata tctcctcctg
//
LOCUS       DAZOYL010000026        24494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZOYL010000026 DAZOYL010000000
VERSION     DAZOYL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.026"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..429)
                     /locus_tag="ACHUOY_000789"
     CDS             complement(<1..429)
                     /locus_tag="ACHUOY_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0681650.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNK"
     gene            530..1129
                     /locus_tag="ACHUOY_000790"
     CDS             530..1129
                     /locus_tag="ACHUOY_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR0681651.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            1237..2004
                     /gene="tpiA"
                     /locus_tag="ACHUOY_000791"
     CDS             1237..2004
                     /gene="tpiA"
                     /locus_tag="ACHUOY_000791"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR0681652.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(2059..2814)
                     /gene="cdh"
                     /locus_tag="ACHUOY_000792"
     CDS             complement(2059..2814)
                     /gene="cdh"
                     /locus_tag="ACHUOY_000792"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR0681653.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(2921..3910)
                     /gene="sbp"
                     /locus_tag="ACHUOY_000793"
     CDS             complement(2921..3910)
                     /gene="sbp"
                     /locus_tag="ACHUOY_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR0681654.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(4230..5192)
                     /gene="pfkA"
                     /locus_tag="ACHUOY_000794"
     CDS             complement(4230..5192)
                     /gene="pfkA"
                     /locus_tag="ACHUOY_000794"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR0681655.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5373..6275)
                     /gene="fieF"
                     /locus_tag="ACHUOY_000795"
     CDS             complement(5373..6275)
                     /gene="fieF"
                     /locus_tag="ACHUOY_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR0681656.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6423..6923)
                     /gene="cpxP"
                     /locus_tag="ACHUOY_000796"
     CDS             complement(6423..6923)
                     /gene="cpxP"
                     /locus_tag="ACHUOY_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR0681657.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7073..7771
                     /gene="cpxR"
                     /locus_tag="ACHUOY_000797"
     CDS             7073..7771
                     /gene="cpxR"
                     /locus_tag="ACHUOY_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR0681658.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7768..9141
                     /gene="cpxA"
                     /locus_tag="ACHUOY_000798"
     CDS             7768..9141
                     /gene="cpxA"
                     /locus_tag="ACHUOY_000798"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR0681659.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9247..9921)
                     /gene="yiiM"
                     /locus_tag="ACHUOY_000799"
     CDS             complement(9247..9921)
                     /gene="yiiM"
                     /locus_tag="ACHUOY_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR0681660.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10070..11053)
                     /gene="kdgT"
                     /locus_tag="ACHUOY_000800"
     CDS             complement(10070..11053)
                     /gene="kdgT"
                     /locus_tag="ACHUOY_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR0681661.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11313..11933)
                     /gene="sodA"
                     /locus_tag="ACHUOY_000801"
     CDS             complement(11313..11933)
                     /gene="sodA"
                     /locus_tag="ACHUOY_000801"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR0681662.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12218..13252
                     /gene="rhaT"
                     /locus_tag="ACHUOY_000802"
     CDS             12218..13252
                     /gene="rhaT"
                     /locus_tag="ACHUOY_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR0681663.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13249..14097)
                     /gene="rhaR"
                     /locus_tag="ACHUOY_000803"
                     /pseudo
     CDS             complement(13249..14097)
                     /gene="rhaR"
                     /locus_tag="ACHUOY_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(14171..15007)
                     /gene="rhaS"
                     /locus_tag="ACHUOY_000804"
     CDS             complement(14171..15007)
                     /gene="rhaS"
                     /locus_tag="ACHUOY_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR0681664.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15295..16764
                     /gene="rhaB"
                     /locus_tag="ACHUOY_000805"
     CDS             15295..16764
                     /gene="rhaB"
                     /locus_tag="ACHUOY_000805"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR0681665.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            16761..18020
                     /gene="rhaA"
                     /locus_tag="ACHUOY_000806"
     CDS             16761..18020
                     /gene="rhaA"
                     /locus_tag="ACHUOY_000806"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR0681666.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            18262..19086
                     /gene="rhaD"
                     /locus_tag="ACHUOY_000807"
     CDS             18262..19086
                     /gene="rhaD"
                     /locus_tag="ACHUOY_000807"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR0681667.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            19096..19410
                     /gene="rhaM"
                     /locus_tag="ACHUOY_000808"
     CDS             19096..19410
                     /gene="rhaM"
                     /locus_tag="ACHUOY_000808"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR0681668.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            19711..20157
                     /locus_tag="ACHUOY_000809"
     CDS             19711..20157
                     /locus_tag="ACHUOY_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR0681669.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            20168..21697
                     /locus_tag="ACHUOY_000810"
     CDS             20168..21697
                     /locus_tag="ACHUOY_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFR0681670.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVFLVCYLANDKALLGTQSGAGFLGAVVLGLAIGYFVFWFRKV
                     RLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAA
                     FLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPK
                     LFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAFGIKR
                     LAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHE
                     H"
     gene            21687..22757
                     /locus_tag="ACHUOY_000811"
     CDS             21687..22757
                     /locus_tag="ACHUOY_000811"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0681671.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            22757..>24494
                     /locus_tag="ACHUOY_000812"
     CDS             22757..>24494
                     /locus_tag="ACHUOY_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR0681672.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELL"
BASE COUNT         5973 a         6414 c         6249 g         5858 t
ORIGIN      
        1 tttattgtcg gctttatact tcacatcctg agtatcttta ccatatttat tttcgccttg
       61 tgtgccaaca aacgcaccaa gatcgataag catcatcacc agaatcaacg tcgggacaaa
      121 acgccccacc gcccattgcc agacacccgg taaaatcgcc cagttacccg ccagcagcat
      181 ccacgccaca atcatcagaa atgcccatgc gccggaacgc ccgcgatcat gcaagcgctt
      241 aacagttact gccgctgttg gccaaagcaa gcacacaagg caaaacgccg cggtctgaat
      301 atcgagtaaa tttttacccg ccagtgagaa aagcaccagc atgcctgcga accacaggcc
      361 tatccaaatc cagaaatcac ggcgtccaat acgcccttta aatgagaata accattgctg
      421 tatggtcatg taagttcctt gatggttgtc ttttccagga ttctaccctt ttgacaaggg
      481 cgacagatat cgttttaatc ggagccagtc ataacaaaag gtactgtcaa tgaagccagg
      541 gtgtacgctg ttttttctct tatgttctgc attaaccgtt acaacaacgg cgcatgcaca
      601 aacaccagat acggcaacga ccgcgcctta tctgctggct ggagccccta ctttcgatct
      661 ctccatcagc cagtttcgag aagactttaa cagccagaat cctagcctgc cactgaacga
      721 atttcgtgcc atcgacagca gtcccgacaa agccaatctc actcgtgctg caagtaaaat
      781 taacgagaac ttgtatgctt ctacagcgct ggagcgcggt accttaaaaa tcaaaagcat
      841 tcaaatgacc tggctaccca tccaggggcc agagcaaaaa gcagcgaaag cgaaagctca
      901 ggaatacatg gcagcagtga tccgcacact caccccatta atgaccaaaa cacaaagcca
      961 gaaaaaactg cagtcgctac taacggcggg gaaaaacaaa cgttattaca ccgagacaga
     1021 aggtgcactg cgttatgttg tcgcggacaa cagcgaaaag gggctgacct tcgctgttga
     1081 accgattaag ctggcgctat ctgaatcgct tgaaggtttg aataaatgac aaaaagcaaa
     1141 gcctttgtgc cgatgaatct ctatactgtt tcacagacct gctgccctgc ggggcggcca
     1201 tcttccttta ttcgcttata agcgtggaga attaaaatgc gacatccttt agtgatgggt
     1261 aactggaaac tgaacggcag ccgccacatg gttcacgagc tggtttctaa cctgcgtaaa
     1321 gagctggcag gtgttgctgg ctgtgcggtt gcaatcgcac caccggaaat gtacatcgat
     1381 atggcgaagc gcgaagctga aggcagccac atcatgctgg gtgcgcaaaa cgtggacctg
     1441 aacctgtccg gcgcattcac cggtgaaact tctgccgcta tgctgaaaga catcggcgct
     1501 cagtacatca tcatcggcca ctctgaacgt cgtacttacc acaaagagtc tgacgaactg
     1561 atcgcgaaaa aattcgcggt gctgaaagag cagggcctga ctccggttct gtgcatcggt
     1621 gaaaccgaag ctgaaaacga agcgggcaaa actgaagaag tttgcgcacg tcagatcgac
     1681 gcggtactga aaactcaggg tgctgcggca ttcgaaggtg cggttatcgc ttacgaacct
     1741 gtatgggcaa tcggtactgg caaatctgca actccggctc aggcacaggc tgttcacaaa
     1801 ttcatccgtg accacatcgc taaagttgac gctaacatcg ctgaacaagt gatcattcag
     1861 tacggcggct ctgtaaacgc gtctaacgct gcagaactgt ttgctcagcc agatatcgac
     1921 ggcgcgctgg ttggcggtgc ttctctgaaa gctgacgctt tcgcagtgat cgttaaagct
     1981 gcagaagcgg ctaaacaggc ttaagtctga caggtgccgg atttcatatc cggcacttac
     2041 tttccttaac tcttcgcctt aacgcaaaat ctcacactga tgatcctgaa tttcctcggc
     2101 tgaagcacgg ttaagcgtca gtagatttcg ttgtgtcgcc agcaatacaa atgagttatc
     2161 actctgccgt accatcgcca gcccgtagct tcccatatgt tcccgcgcct caggtacttc
     2221 ttctgccagc atcataaatg ggctgcgttg taccagttcg ctttccgtta cccgacgcgc
     2281 caggtattca tgcccacgca aaccacctgg cagtggcaac cagcggctgc tgatgttcgc
     2341 cagattgtta tccagttgtt cgcgcacatc aggacgaata caagagatat gaatatgaaa
     2401 atggttttgc gtacgcccgg tgcgggagtt gatcgccaaa gaaaccgcgc gatcgggaac
     2461 cggctggccg tattttttgc tcataaaatc acgcgcctgc caggccaacc aaaagaagtt
     2521 cggcgttgaa ggatcggtca acaaaggact ttcagtaccg ttaatacgat acgttggcat
     2581 caacagatat tgcagtggac cattaagatc ttttaaaacc acgtatccgg cattgggttt
     2641 gacttccgca catggcgaag gattttgatt ttgctgctga ttgggcaaac attcctcaag
     2701 gacaatctta cgtaatgtat ccgactcttc accggttaat ttccagtaac caataccggc
     2761 agccacaacg gcgataacta tcatcaccaa aaaaagaaga cccgcttttt tcatcttttt
     2821 ttccctgtac ctcaaagaga tgcaagggta acgcaaaatc gtgacaaata aaaaacccgg
     2881 tcgcgagaaa ccaccgggtt tgataattat cctggggaaa tcagcgtttg ctgatctgat
     2941 cgaacgtacc gccgttagca aaatgctctt tttgcgcttt cgtccagccg ccgaactctt
     3001 catcaatggt gaataacttc agctttggaa acgcattttc gtactttttc gctacttcag
     3061 cgtcgcgcgg acggtagtag tttttcgcgg caatttcctg accttctggc gagtagagat
     3121 atttcaggta ggcttccgcc acctctttgg tgcctttttt ctcgaccact ttatcgacca
     3181 ccgacacggt tggctctgcg aggatagact cactcggcgt gacgatttcg aatttatctt
     3241 tccccagttc attcgctgcc agcagagctt cgttttccca ggcgatcagt acatcgccaa
     3301 tcccgcgctc gacaaaggtg ttagtggagc cgcgcgcgcc agaatccaga acttcgacgt
     3361 ttttatacag tgcccgaaca aaatcctgtg cttttgcctg atcgttgttg ttgtgatgca
     3421 gcgcgtagcc ccaggcagct aggtagttcc agcgtgcgcc accagagctt ttcggattag
     3481 gcgtgatcac cgaaacaccc ggtttaatca gatcgttcca gtcatggatc tgcttcggat
     3541 tgcccttacg caccaggaaa acaatggtgg aagtgtacgg tgcggagtta tccggcagac
     3601 gtttgatcca ctctttatca atccgcccac gttccgcaat cgcgtccacg tcataggcca
     3661 gagccagcgt gacaacatca gcttcaatac cgttgattac cgacgtcgct tgtttacctg
     3721 aaccaccgtg tgactgacga atcaccacgt tatcaccagt ttgctgtttc cagtgggcgc
     3781 tgaatgcctt gttgtactgt tcgtacaatt cgcgcgttgg atcatatgaa acgttaagaa
     3841 gctgaatatc ctttgccata acgctggttg ccgccagcaa aaatgttaac cctacgcccc
     3901 acttgttcat cgcccgactc tcttatgttg tgttgtgatg agcaaagcgt gccagaaggt
     3961 taaccaaaca ttaaagaata aaaaaagatt ggctataact tgcgggtata tgttgaggga
     4021 ttaaaaaggc ggaagaaatc cgcctcatat tgctgacaaa gtgcgctttg tccatgccgg
     4081 atgcggcgcg aacgccttat ccggcctaca aaagtttgca aattcaataa attgcagaat
     4141 tcatgtaggc ctgataagcg aagcgcatca ggcatttttg cttctgtcat cggtttcagg
     4201 ctaaaggaat ctgccttttt ccgaaatcat taatacagtt ttttcgcgca gtccagccag
     4261 tcacctttga acggacgctt catgttttcg atagcgtcga tgatgtcgtg gtgaaccagc
     4321 tgttcgttct ggatacctac acaacgaccg ccgtaacctg ccagcagcag atcgatagcg
     4381 taagcgccca tacgggaagc cagaatacgg tcgtaaggca ccggagaacc accgcgctgg
     4441 atgtggccca gcacagttgc gcgggtttca cgaccggttt ctttctcgat gaaatgcgcc
     4501 agttcgtcaa catcacacat atgttcggta atcgccacga tcgcgtgttt tttacctttc
     4561 gcgatacccg ctttgatttc gtttaccagg tcttcacggc tgaattcaac ttccggaacc
     4621 acaacgaatt cacagccacc ggcaatggcc gcagccaacg tcagatcgcc acaataacgg
     4681 cccatcactt ccaccacgga aatacgctgg tgagaagaag aggtgtcacg cagacggtcg
     4741 atcgcttcta caacggtgct cagcgcagtg aagaaaccga tagtgtagtc agtgcctttg
     4801 atgtcgttgt cgatagtgcc cggcagaccg atgcacggga agcccatttc ggtcagacgc
     4861 attgcaccca tgtaggaacc gtcaccgccg ataaccacca gcgcgtcgat accacgtttt
     4921 ttcaggtttt cgatagccac ggcgcggatg ttctcgtcgc ggaattccgg gaagcgcgca
     4981 gaaccgagga acgtaccgcc acggttgatc atgtcagaaa cgctgtaacg gtctagctgt
     5041 accatacggt cttcatacag acccagatag ccgtcataaa tgcccattac ttccagacct
     5101 tctgtcagcg cagaacgaac aaccccgcga attgcggcgt tcatgcctgg cgcatcaccg
     5161 ccgcttgtca acacaccgat tttcttaatc atgactacct ctgaactttg gaatgcaaaa
     5221 taaaatctgt tgccggaagt cttcttgcac atcgaagtga tccaacgaat gtgcaaatag
     5281 tataacaatc acttcctgct gaattgattc aggtcaggcc aaatggcggt attttataca
     5341 caaaatgcgg gtctggctct cttttatact gattatgaaa gcatagaccg tttaccctcc
     5401 ctgggtacga cggaacaggg gtcctgatgg ataattacat ccgatcccgg aaaacgccgt
     5461 aaaatagcct gctctacctg atccgccacc atatgtgcct gaaccaaagg cagagagtct
     5521 tccatttcca aatgaatctg aataaagcgg gtcggccctg actgccgcgt gcgaagatcg
     5581 tgagcgccgc taacacccgg ccaggaagtc acgatatcaa taatttcttg ccgttcctca
     5641 tcaggcaatg cgcgatccag taatgactgt accgcctcat atcccatgcg taacgcgcta
     5701 tataaaatat agatgccgat tcccaatgca aacagagcat cggcgcgatg ccagccgtac
     5761 caggacaacc ccagcgccag cagaattgcg ccgttcatca taacatcaga ctggtaatgt
     5821 agcatatcag cccgcaccgc ctggctttgc gtccggcgca ccacccaccg ctgaaacgag
     5881 acaaggataa tcgtacaaat tagcgccaca attgtcacga taaccccgac gcctggatct
     5941 gtcatcggtg ttggagatac cagatgttga atacccgtca aaaacaggaa tagtgccgaa
     6001 ccggagataa acatactttg cgccagcgcc gcgagggact cagctttacc gtgaccaaac
     6061 gagtgattat cgtcggcagg ttgcagggaa tatcgcacca ccagtaaatt cgtcaacgac
     6121 gcgccgatat ccaccagcga atccaccagc gcggcgagaa tactcaccga cccggtatac
     6181 caccatgcaa aaattttaat cagcaatagc agcgaagcca tcgccgtcgc agcaatcgcc
     6241 gcccgactga ccagccgtcc ataagattga ttcataaata ctcccactat caactgacgc
     6301 tagtataacg gaagcaaatc atctgcaatg cattaagcag caggcaaatt gaggataaaa
     6361 aaaaccccac atcatgtggg ggaagacagg gatggtgtct atggcaagga aaacagggtt
     6421 tactactggg aacgtgagtt gctactactc aatagcttca acgatgaact tttttgccat
     6481 tgcgtcacgt cacgcaactg ctccattcgt tgttgatgtt tctcgtttaa aaccgcttgc
     6541 tgctccggcg ttaacaggcg atacatttgg ttgcggactt ttgccatctc aacctgacga
     6601 gcaatttgct cattcgccat tttttctgcc tgtgcgcgca cagcgttttc atcaaaattt
     6661 tctgcggtga caaggcgatg cattgtctcc agttcgctaa cattaacagg aggctgttcg
     6721 tgccgggcct gttgcataag atctcgcatc tgctgacgct gatgttcggt taaacttatg
     6781 ccgtcgaaca tatggctctg cgtactgcgc tgcgtaagtt cttcacccgg atgccagtta
     6841 tcgcctgaac cgacttcagc agcgtggctt aatgaactga ctgccagcgt tgaggccatg
     6901 acggcagcgg taactatgcg catcatttgc tcccaaaatc tttctgtcgc gattcaacga
     6961 tagagagttt acgattcagg ctgcaaacat gcgtcagggg gtgtaaaaca acgtaaagtc
     7021 atggattagc gacgtctgat gacgtaattt ctgcctcgga ggtatttaaa caatgaataa
     7081 aatcctgtta gttgatgatg accgagagct gacttcccta ttaaaggagc tgctcgagat
     7141 ggaaggcttc aacgtgattg ttgcccacga tggggaacag gcgcttgatc ttctggacga
     7201 cagcattgat ttacttttgc ttgacgtaat gatgccgaag aaaaatggta tcgacacatt
     7261 aaaagcactt cgccagacac accagacgcc tgtcattatg ttgacggcgc gcggcagtga
     7321 acttgatcgc gttctcggcc ttgagctggg cgcagatgac tatctcccga aaccgtttaa
     7381 tgatcgtgag ctggtggcac gtattcgcgc gatcctgcgc cgttcgcact ggagcgagca
     7441 acagcaaaac aacgacaacg gttcaccgac actggaagtt gatgccttag tgctgaatcc
     7501 aggccgtcag gaagccagct tcgacgggca aacgctggag ttaaccggta ctgagtttac
     7561 cctgctctat ttgctggcac agcatctggg tcaggtggtt tcccgtgaac atttaagcca
     7621 ggaagtgttg ggcaaacgcc tgacgccttt cgaccgcgct attgatatgc acatttccaa
     7681 cctgcgtcgt aaactgccgg atcgtaaaga tggtcacccg tggtttaaaa ccttgcgtgg
     7741 tcgcggctat ctgatggttt ctgcttcatg ataggcagct taaccgcgcg catcttcgcc
     7801 atcttctggc tgacgctggc gctggtgttg atgttggttt tgatgttacc caagctcgat
     7861 tcacgccaga tgaccgagct tctggatagc gaacagcgtc aggggctgat gattgagcag
     7921 catgtcgaag cggagctggc gaacgatccg cccaacgatt taatgtggtg gcggcgtctg
     7981 ttccgggcga ttgataagtg ggcaccgcca ggacagcgtt tgttattggt gaccaccgaa
     8041 ggccgcgtga tcggcgctga acgcagcgaa atgcagatca ttcgtaactt tattggtcag
     8101 gccgataacg ccgatcatcc gcagaagaaa aagtatggcc gcgtggaact ggtcggtccg
     8161 ttctccgtgc gtgatggcga agataattac caactttatc tgattcgtcc ggccagcagt
     8221 tctcaatccg atttcattaa cttactgttt gaccgcccgc tattactgct gattgtcacc
     8281 atgttggtca gtacgccgct gctgttgtgg ttggcctgga gtctggcaaa accggcgcgt
     8341 aagctgaaaa acgctgccga tgaagttgcc cagggaaact tacgccagca cccggaactg
     8401 gaagcggggc cacaggaatt ccttgccgca ggtgccagtt ttaaccagat ggtcaccgcg
     8461 ctggagcgca tgatgacctc tcagcagcgt ctgctttctg atatctctca cgagctgcgc
     8521 accccgctga cgcgtctgca actgggtacg gcgttactgc gccgtcgtag cggtgaaagc
     8581 aaggaactcg agcgtattga aaccgaagcg caacgtctgg acagcatgat caacgatctg
     8641 ttggtgatgt cacgtaatca gcaaaaaaac gcgctggtta gcgaaaccat caaagccaac
     8701 cagttgtgga gtgaagtgct ggataacgcg gcgttcgaag ccgagcaaat gggcaagtcg
     8761 ttgacagtta acttcccgcc tgggccgtgg ccgctgtacg gcaatccgaa cgccctggaa
     8821 agtgcgctgg aaaacattgt tcgtaatgct ctgcgttatt cccatacgaa gattgaagtg
     8881 ggctttgcgg tagataaaga cggtatcacc attacggtgg acgacgatgg tcctggcgtt
     8941 agcccggaag atcgcgaaca gattttccgt ccgttctatc ggaccgatga agcacgcgat
     9001 cgtgaatctg gcggtacagg attggggctg gcgattgttg aaaccgccat tcagcagcat
     9061 cgtggctggg tgaaggcaga agacagcccg ctgggcggtt tacggctggt gatttggttg
     9121 ccgctgtata agcggagtta aactccgcat ttgtaggcag gataaggcgt ttacgccgca
     9181 tccggcattt gagcaggatg cctgatgcga cgctgatagc gtcttatcag gcctacactc
     9241 cgggggttat tttccccaca accggcggga aaaatcttcg atcttgccgc tcagtcggcg
     9301 cttttgcatc gtccgcgtcc agcttttcga taacccggcg gcggagagta aacggtgata
     9361 ctgatcgtca tcaaacggca tatgccatgc gatggcggca gcctcctgca cggtcacatc
     9421 gctgacacgg gaaaccaact ccagcggcgc gtccgcagac actttccctg gcgctatcac
     9481 gctatacaac caccccacct tacctgtgtt ttgcatcaac tgcgctatat cgctgatatc
     9541 aaaatggtaa ttgagtttat agcaaggcga gcgcggctgg ctgacctgaa ttaatgcctc
     9601 tccccagcgg aaaatatcgc ccatatgaac attactttcc gtgagaccgt cggttgagag
     9661 attttcgcca aacgcaggcg caacaaacaa ctccgcctgt tccggaaatt cccgcaccca
     9721 gtagagatag tgctcacgag gataatgaca cagcgctctg tctggcccgc cgtgaacctt
     9781 tttctccgcc tgctcgtcac cttccagccc cagctccgtc agcatcaact caccatcaac
     9841 ctggattttg gcaattgcgc tgggtttgcc ttcgggataa gcctgaatct tgcctgtgta
     9901 tacatcaacc ggatatcgca tctgttccct ctccacgaga aatttcccca ttagaccata
     9961 tttattttat gtcaggcgtg ctgttttcgc ggtaagtatc caggcaggaa ctcaatagca
    10021 aaccggacga acgttagttc atccggctgt ggggattaag gaagcaggtt tatttcaccg
    10081 tacctaaaat ttcgattttc gctgctctgg ctttgacttt acgtgaccag atagaggtaa
    10141 gaattggcac cagaatcgaa gtcacaatga ccgccgtcgc taccagcgaa gttgctgccg
    10201 gagccatcgg tttaaacgca ggcaccattt ctgcaatcag cacaggtgtc gctaccgcgg
    10261 cccctgcgga actggaagcg gcaattccgg cagtgccatc gccaccgcca atcaatttat
    10321 cggcgataat caacggaata ccggtcacga taattactgc cacacccaac aggatcccca
    10381 gcaaaccagt ctgggcaatc acagtcaaat caatggtatt gcccagcgcg aaggcaaaga
    10441 atggaatcag cgtttgcact gctttgctga aaaattctcg taattcaggg tcaagattcc
    10501 ccagggcaaa gcccaccagg aacggtaata ctgcgccgac gaaaacatgc ggttcaaacg
    10561 aggcaatccc ggcagtgccc agaataatca tcgtcatgag cggcccggac tccaacgaca
    10621 tcaacacaaa tgccccagct tcttctttcg taccgtactg ctgcatgatg gaagcgtaaa
    10681 gtccgccgtt ggtcatatcc atcgccgcca ccagcgccag cgttgaaagt ccggcaaaga
    10741 atccaacttc aacaccatgt tccggaataa tgcgcgaggc aattgccgca accacccacg
    10801 cgacggcaat tttagttacc accagcgtac cggatttacg cagtaccgtt cccgtcgcgc
    10861 ttaattttat tgacgccccc atgcaaaaaa accacaccgc cagaatgggc accgtaccgg
    10921 taatcatccc gttggtgaat gatccaaaat atttccccgc ccccggcgag aaggtgtggc
    10981 acagtgcgcc aaggaatagc gggacgagca tcatcccccc cgggattttc tcaatcgagc
    11041 gttttatctg catttccatc acctgtcggc atatttttaa gggctgatgc gtaattcctc
    11101 aatggagcat cagtgttgat ggaaacgaag atagacattc atttaatcaa taaaagtgat
    11161 caaaaccaca tcaattgaaa cgctgtttta tttttatcgg atcattgttt ttattttgaa
    11221 attgatcaca aaaaaaccac cgccgttggc gatggttcat tttttaagct gatatgcggc
    11281 ctatacgcct cattgcagca ggcggcaaat gattattttt tcgccgcaaa acgtgccgct
    11341 gcttcgtccc agttcaccac gttccagaac tctttaatgt agtccggacg gcggttctgg
    11401 aatttcaggt agtaagcatg ttcccacaca tccaggccca gaatcgggaa gccggaagcg
    11461 ccagaaatag cttcacccat cagcggagaa tcctggttag cagtagaaac caccgccagt
    11521 ttatcgcctt tcagcaccag ccatgcccag ccggaaccaa agcgggaagc tgccgctttt
    11581 tcaaattctg ctttgaagtt atcaacggag ccgaagtcac gttcgatagc cgctttcagg
    11641 tcaccctgca gggtggtgcc ttttttcaga cctttccaga acaggctgtg gttagcgtga
    11701 ccgccagcgt tgttgcgcag tacggttttc ttgtctgctg gcagctggtc cagtttggtg
    11761 atcagctctt caaccggcag gttggcaaat tctggcaggc tttccagcgc cgcgttggcg
    11821 ttgtttacgt aggtctgatg gtgtttggtg tggtggattt ccatggtctg cttatcgaag
    11881 tgcggttcca gggcatcgta agcatacggc agggatggca gggtatagct catattcatc
    11941 tccagtattg tcgggcggcc gattgttaat gccgcgtaag cagttggttc attatagtta
    12001 attaaatgat attgaaaatg attatcaatg ccgtactttt cgtaagggta tggttttgca
    12061 ggaaaatgcc cgagatgtga agcaaatcac ccacttaatg ccgtgattgc cagtaaatcg
    12121 acaacggcgg caacaggcga aaggttaatc gacagcacga tttttacact catctcgtcg
    12181 gagatgtgaa gcgacgaaaa atgatgagga taagaagatg agtaacgcga ttacgatggg
    12241 gatattttgg catttgatcg gcgcggccag tgcagcctgt ttttacgctc cgttcaaaaa
    12301 agtaaaaaaa tggtcatggg aaaccatgtg gtcagtcggt gggattgttt cgtggattat
    12361 tctgccgtgg accatcagcg ccctgttact accggatttc tgggcgtatt acagctcgtt
    12421 tagtctctct acgctgctgc ctgtttttct gttcggcgct atgtggggga tcggtaatat
    12481 caactatggc ctgaccatgc gttatctcgg catgtcgatg ggaattggca tcgccattgg
    12541 cattacgttg attgtcggta cgctgatgac gccaattatc aacggcaatt tcgatgtgct
    12601 gattaacacc gaaggcggac gcatgacgtt gctcggcgtt ctggtggcgc tgattggcgt
    12661 agggattgta actcgagccg ggcagttgaa agagcgcaag atgggcatta aagccgaaga
    12721 gttcaatctg aaaaaagggc tggtgctggc ggtgatgtgc ggcattttct ctgccgggat
    12781 gtcctttgcg atgaacgccg caaaaccgat gcatgaagcc gctgccgcac ttggcgtcga
    12841 tccactgtat gtcgctctgc caagctatgt tgtcatcatg ggcggcggcg cgatcattaa
    12901 cctcggtttc tgttttattc gtctggcaaa agtgaaggat ttgtcgctaa aagccgactt
    12961 ctcgctggca aaaccgctga tcattcacaa tgtgttactc tcggcactgg gcgggttgat
    13021 gtggtatctg caattctttt tctatgcctg gggccacgcc cgcattccgg cgcagtatga
    13081 ctacatcagt tggatgctgc atatgagttt ctatgtattg tgcggcggta tcgtcgggct
    13141 ggtgctgaaa gagtggaaca atacaggacg ccgtccggta acggtgttga gcctcggttg
    13201 tgtggtgatt attgtcgccg ctaacatcgt cggcatcggc atggcgaatt aatctttctg
    13261 cgaattgaga tgacgccact ggctgggcgt catcccggtt tcccgggtaa acaccaccga
    13321 aaaatagtta ctatcttcaa agccacattc ggtcgaaata tcactgatta acaggcggct
    13381 atgctggaga agatattgcg catgacacac tctgacctgt cgcagatatt gattgatggt
    13441 cattccagtc tgctggcgaa attgctgacg caaaacgcgc tcactgcacg atgcctcatc
    13501 acaaaattta tccagcgcaa agggactttt caggctagcc gccaaccggg taatcagctt
    13561 atccagcaac gtttcgctgg atgttggcgg caacgaatca ctggtgtaac gatggcgatt
    13621 cagcaacatc accaactgcc cgaacagcaa ctcagccatt tcgttagcaa acgacacatg
    13681 ctgactactt tcatgctcaa gctgaccgat aacctgccgc gcctgcgcca tccccacgct
    13741 acctaagcgc cagtgtggtt gccctgcgct ggcgctaaat cccggaatcg ctccctgtca
    13801 gtcaagattc agcttcagcc gctccgggca ataaataata ttctgcaaaa ccagatcgtt
    13861 aacggaagcg taggagtgtt tatcgtcagc atgaatataa aagagatcgc cacgggtaat
    13921 gcgataagga cgatcgttga gtacatgcag accattaccg cgccagacaa tcaccagctc
    13981 acaaaaatca tgtgtatgtt cggcaaagac atcttgcgga taacggtcag ccacagcgac
    14041 tgcctgctgg tcgctggcaa aaaaatcatc tttgagaagt tttaactgat gcgccaccgt
    14101 ggctacctcg gccagagaac gaagttgatt attcgcaata tggcgtacaa atacgttgag
    14161 aagattcgcg ttattgcaga aagccatccc gtccctggcg aatatcacgc ggtgaccagt
    14221 taaactctcg gcgaaaaagc gtcgaaaagt ggttactgtc gctgaatcca cagcgatagg
    14281 cgatgtcagt aacgctggcc tcgctgtggc gtagcagatg tcgggctttc atcagtcgca
    14341 ggcggttcag gtatcgctga ggcgtcagtc ccgtttgctg cttaagctgc cgatgtagcg
    14401 tacgcagtga aagagaaaat tgatccgcca cggcatccca attcacctca tcggcaaaat
    14461 ggtcctccag ccaggccaga agcaagttga gacgtgatgc gctgttttcc aggttctcct
    14521 gcaaactgct tttacgcagc aagagcagta attgcataaa caagatctcg cgactggcgg
    14581 tcgagggtaa atcattttcc ccttcctgct gttccatctg tgcaaccagc tgtcgcacct
    14641 gctgcaatac gctgtggtta acgcgccagt gagacggata ctgcccatcc agctcttgtg
    14701 gcagcaactg attcagcccg gcgagaaact gaaatcgatc cggcgagcga tacagcacat
    14761 tggtcagaca cagattatcg gtatgttcat acagatgccg atcatgagcg cgtacgaaac
    14821 agaccgtgcc accggtgatg gtatagggct gcccattaaa cacatgaata cccgtgccat
    14881 gttcgacaat cacaatttca tgaaaatcat gatgatgttc aggaaaatcc gcctgcggga
    14941 gccggggttc tatcgccacg gacgcgttac cagacggaaa aaaatccaca ctatgtaata
    15001 cggtcatact ggcctcctga tgtcgtcaac acggcgaaat agtaatcacg aggtcaggtt
    15061 cttaccttaa attttcgacg gaaaaccacg taaaaaacgt cgatttttca agatacagcg
    15121 tgaattttca ggaaatgcgg tgagcatcac atcaccacaa tgaagctaat tgtgaacatc
    15181 atcacgttca tctttccctg gttgccaatg gcccattttc ctgtcagtaa cgagaaggtc
    15241 acgaattcag gcgcttttta gactggtcgt aatgaaattc agcaggatca cattatgacc
    15301 tttcgcaatt gtgtcgccgt cgatctcggc gcatccagtg ggcgcgtgat gctggcgcgt
    15361 tacgagcgtg aatgccgcag cctgacgctg cgcgaaatcc atcgttttaa caatggcctg
    15421 catagccaga acggttatgt cacctggaat gtggatagcc tggaaagtgc cattcgcctt
    15481 ggattaaaca aggtgtgcga ggaagggatt cgtatcgata gcattgggat tgatacctgg
    15541 ggcgtggact ttgtgctgct cgaccaacag ggtcagcgtg tgggcctgcc cgttgcttat
    15601 cgcgatagcc gctccaatgg cctaatggcg caggcacagc aacaactcgg caaacgcgat
    15661 atttatcaac gtagcggcat ccagtttctg cccttcaata cgctttatca actgcgtgcg
    15721 ctgacggagc aacaacctga acttattcca cacattgctc acgctctgct gatgccggat
    15781 tacttcagct atcgcctgac cggcaagatg aactgggagt acaccaacgc cacgaccacg
    15841 caactggtca atatcaatag cgacgactgg gacgagtcgc tactggcgtg gagcggggct
    15901 aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg tcattgggca ctggatttgc
    15961 ccgcagggta atgagattcc ggtggtcgcc gttgccagcc atgataccgc cagcgcggtt
    16021 atcgcctcgc cgttaaacgg ttcacgcgct gcttatctct cttctggcac ctggtcattg
    16081 atgggcttcg aaagccagac gccatttacc aatgacacgg cgctggcagc caacataacc
    16141 aatgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa atattatggg cttatggctg
    16201 cttcagcgag tgcttcagga gcggcaaatc aacgatctcc cggcgcttat cgccgcgaca
    16261 caggcacttc cggcctgccg cttcatcatc aatcccaatg acgatcgctt tattaatcct
    16321 gaggcgatgt gcagcgaaat tcaggctgcg tgtcgggaaa tggcgcaacc gctcccggaa
    16381 agtgatgctg aactggcgcg ctgcattttc gacagtctgg cgctgctgta tgccgatgtg
    16441 ttgcatgagc tggcgcagct acgcggtgaa gatttctcgc aactgcatat tgtcggcggc
    16501 ggctgccaga acacgctgct caaccagcta tgtgccgatg cctgcggtat tcgggtgatc
    16561 gccgggcctg ttgaagcctc gacgctcggc aatatcggca tccagttaat gacgctggat
    16621 gaactcaaca atgtggatga tttccgtcag gtcgtcagca ccaccgcgaa tctgaccacc
    16681 tttaccccta atcctgacag tgaaattgcc cactatgtgg cgcagcttca ctcgacacga
    16741 cagacaaagg agctttgcgc atgaccactc aactggaaca ggcctgggag ctggcgaaac
    16801 agcgtttcgc ggcggtgggg attgatgtcg aggaggcgct gcgccaactt gatcgtttac
    16861 ccgtttcaat gcactgctgg cagggcgatg atgtttccgg ttttgaaaac ccggaaggtt
    16921 cgctgaccgg tgggattcag gctattggta attatccggg caaagcgcgt aatgccagtg
    16981 agctacgagc cgatctggaa caggctatgc ggctgattcc ggggccgaaa cggcttaatt
    17041 tacatgccat ctatctggaa tcagacacgc cagtctcgcg cgaccagatc aaacctgagc
    17101 acttcaaaaa ctgggttgaa tgggcgaaag ccaatcagct cggtctggat tttaacccct
    17161 cctgtttctc gcatccgcta agcgccgatg gctttacgct ctcgcatccc gacgacagca
    17221 ttcgccagtt ctggattgat cactgcaaag ccagccgtcg cgtttcggcc tattttggcg
    17281 agcaactcgg cacaccatcg gtgatgaaca tctggatccc ggatggtatg aaagatatca
    17341 ccgttgaccg tctcgccccg cgtcagcgtc tgctggcagc actggatgag gtgatcagcg
    17401 agaagctgga tccggcgcac catatcgacg ccgttgagag caaattgttt ggcattggcg
    17461 cagagagcta cacggttggc tccaatgagt tttacatggg gtatgccacc agccgccaga
    17521 ctgcgctgtg cctggacgcc gggcatttcc acccgactga agtgatttcc gacaagattt
    17581 ccgccgccat gctgtatgta ccgcagttgc tgctgcacgt cagccgtccg gttcgctggg
    17641 acagcgatca cgtggtgctg ctggatgatg aaacccaggc gattgccagt gagattgttc
    17701 gtcacgatct gttcgaccgg gtgcatatcg gcctcgactt ctttgatgcc tctatcaacc
    17761 gcattgccgc gtgggtcatt ggtacacgca atatgaaaaa agccctgctg cgtgcgttgc
    17821 tggaacctac cgctgagctg cgcaagctgg aagcggcggg cgattacact gcgcgtctgg
    17881 cgctactgga agaacagaaa tcgttgccgt ggcaggcggt ctgggaaatg tattgccaac
    17941 gtcacgatac gccagcaggt agcgaatggc tggagagcgt gcgggcttat gagaaagcga
    18001 ttttgagcca gcgtgggtaa acactgccgg atgcggcgcg agcgccttat ccggcctaca
    18061 ggtcgaatga ccatgcgtgc tgccaccctc accccggccc tctccctgaa agggcgaggg
    18121 agaaaaacat agcctcgtgc ggcaacggag ctgaacaccg ctccggacag tcccctcgcc
    18181 cctacgggga gagggttagg gtgaggggaa caagccgcac catgtctaaa caaggcaacc
    18241 taatttacag gaacacagaa catgcaaaac attacccagt cctggtttgt ccagggaatg
    18301 atcaaagcca ccaccgatgc ctggctgaaa ggctgggacg agcgcaatgg tggtaacctg
    18361 acgctgcgcc tggatgacgc cgatatcgcg ccttatcacg gcaactttca tgcacaaccg
    18421 cgctatattc ccctcagcca acccatgcct ttactggcaa atacgccgtt tattgtcacc
    18481 ggctccggca aattcttccg taacgtccag cttgatcctg cagctaactt aggcgtcgta
    18541 aaagtcgaca gcgacggcgc gggctaccat attctctggg ggttaaccaa cgaagccgtt
    18601 ccaacttccg aacttccggc tcacttcctt tcccactgcg agcgcattaa agccaccaac
    18661 ggtaaagatc gggtgatcat gcactgccac gccaccaacc tgatcgccct cacctatgta
    18721 cttgaaaacg acaccgcggt ctttactcgc caactgtggg aaggcagcac cgagtgtctg
    18781 gtggtattcc cggatggcgt tggcattctg ccgtggatgg tgcccggcac ggacgaaatc
    18841 ggccaggcga ccgcacaaga gatgcaaaaa cattcgctgg tgttgtggcc cttccacggc
    18901 gtcttcggca gcgggtcgac gttggatgaa acattcggtt taatcgacac cgcagaaaaa
    18961 tcagcacaag tattagtgaa ggtttattcg atgggcggca tgaaacagac catcagccgt
    19021 gaagagttga tagcgctcgg caagcgcttc ggcgttacac cactagctag tgcgctggcg
    19081 ctgtaaggag caaacatgat ccgcaaagcc tttgtcatgc aggttaaccc cgacgcccac
    19141 gaagagtatc agcgtcggca taaccccatc tggccggaac tggaagcagt gttgaaatct
    19201 cacggtgcgc ataactacgc catttacctc gacaaagcgc gtaatctgct gtttgccacg
    19261 gtagagattg aatctgaaga acggtggaat gcggtagcca gcactgatgt ttgccaacgt
    19321 tggtggaaat atatgaccga tgttatgccc gctaacccgg ataacagccc ggtgagtagc
    19381 gagctgcaag aagtgtttta cctgccgtaa ttgcccaatg tgccagagat gcctccggca
    19441 caatatttta aatttcgatt tgaaaaatta aacatattac atctcccggt aagttatatt
    19501 tccctgatac attgtgagta aatcacaaaa ataatgaata acccattaat gattcatgtg
    19561 gtttatttaa ataaccatta tgtgcattac tccgcaaatc tgacctttca ctctgtctca
    19621 aattgtcatt gtttaccgac atatcgcctc tcatcactta atcgctccac cattacaatt
    19681 gattaaaata tattttatgg agtccgatta atggcagctc ttactgcaag ctgtattgac
    19741 ctgaatattc agggcaatgg cgcttattcc gttctgaagc agttggcgac aatagcgtta
    19801 caaaacggtt ttatcaccga ctcacaccag ttcctgcaaa ccctgctgct gcgcgaaaaa
    19861 atgcactcta ctggatttgg ttccggtgtc gccgtgccgc acggtaaaag cgcctgcgtt
    19921 aaacaaccgt ttgtattatt cgcccgcaaa gcgcaggcta ttgactggaa agccagcgat
    19981 ggcgaagacg tcaattgctg gatctgcctc ggcgtgccgc aaagcggcga agaggatcag
    20041 gtcaaaatca tcggcacact gtgtcgcaaa attatccacc aggattttat tcatcaactg
    20101 caacagggcg acgccgacca ggtgcttgcc ttgttaaatc aaaccctcag ctcataagga
    20161 agtggcgatg gagtcatcct tatgtattgt cgcgatcacc aactgccccg ccgggatcgc
    20221 tcacacctac atggtggcgg aagccctgga acagaaagcc cgttctctcg gccataccat
    20281 aaaagtggaa actcaagggt ccagtggcgt tgaaaaccgc ttatccagcg aagagattgc
    20341 cgctgccgat tacgtcattc tcgctaccgg gcgtggcctg agcggtgatg atcgcgcgcg
    20401 gtttgccggg aagaaagttt atgagattgc catctcccag gcgttgaaaa atatcgacca
    20461 gattttcagc gaattaccga caaactcgca gctttttgcc gcagatagcg gcgtgaagct
    20521 tggcaaacag gaagtgcaaa gcggcagcgt gatgagccac ctgatggcgg gcgtctctgc
    20581 ggcgctgccg tttgtcatcg gcggtggtat cctggtggcg cttgccaaca tgctggtgca
    20641 gttcggtttg ccgtataccg atatgtcgaa aggcgcgccg tcgtttacct gggtagttga
    20701 atccatcggt taccttggct tcacctttat gatccccatc atgggcgctt atattgcctc
    20761 gtcgattgcc gataaaccgg ctttcgctcc ggcgtttctg gtttgctatc tggcgaacga
    20821 caaagcgctg ctcggcaccc agtcgggtgc gggattcctc ggcgcggtgg tgtttctggt
    20881 ttgctatctg gcgaacgaca aagcgctgct cggcacccag tcgggtgcgg gattcctcgg
    20941 cgcggtggtg ctggggctgg cgattggcta tttcgtcttc tggttccgca aagtgcgcct
    21001 tggcaaagca ttacaaccgc tgctcggttc aatgctgatc ccgttcgtta ctctgctggt
    21061 tttcggcgtg ctcacttact acgttatcgg gccggtgatg tccgacctca tgggcgggct
    21121 gctccacttc ctgaatacca ttcctccatc aatgaagttt gcggcagcgt ttctggtcgg
    21181 tgcgatgctg gcgttcgata tgggcggccc catcaataaa accgcctggt tcttctgctt
    21241 ctcactgctg gaaaaacaca tttatgactg gtacgccatc gtcggcgttg tcgcgttgat
    21301 gccgcccgtt gcggcgggtc ttgcgacgtt cattgcgccc aaactgttta cccgacagga
    21361 aaaagaggct gccagtagtg ccattgtggt cggtgccaca gtcgcaaccg aaccggctat
    21421 accttacgct ctggcagctc cgttgccgat gattaccgcc aatacgctgg cgggtggcat
    21481 taccggcgtg ctggtgatcg ctttcggaat caaacgtctg gcacctggtt tgggtatctt
    21541 tgacccgctg atcggcctga tgtcgccggt gggttcgttt tatctggtgc tagcgattgg
    21601 cctcgcgctg aacatttcat tcattatcgt cctgaaagga ttgtggttac gccgcaaagc
    21661 gaaagccgcg caacaggagc ttgtccatga acattgagtt actgcaacag ttgtgcgaag
    21721 ccagcgccgt cagcggcgat gaacaggaag ttcgcgacat tctgataaac acgctggaac
    21781 cttgcgttaa tgagatcacc tttgatggtc tgggcagctt tgttgcccgt aaggggaata
    21841 aaggtccaaa agttgccgtt gtcaggcata tggatgaagt cggctttatg gtcacccaca
    21901 tcgacgagag cggttttctg cgctttacca ccattggcgg ctggtggaat cagtcaatgc
    21961 tcaaccaccg ggtaaccata cgcacacaca agggatttaa aatccctggt gtgattggtt
    22021 ccgtcgcacc tcatgcgtta acggaaaagc aaaagcaaca gccgctgtca tttgatgaga
    22081 tgttcattga tattggcgcg aacagtcgcg atgaggtgga aaaacgcggt gtggcgatgg
    22141 gcgattttat tagcccggaa gccaattttg cctgctgggg cgaagataaa gtggtcggca
    22201 aggcgctgga taaccgcatc ggctgcgcaa tgatggctga actattgcag acggtgaata
    22261 atcccgaaat tacgctgtat ggcgtcggca gcgtggaaga agaagttggg ctacgcgggg
    22321 cacaaacctc ggcggaacac attaaaccgg atgtggtgat tgtgctggat accgctgtcg
    22381 caggtgatgt tccgggcatt gataacatta aatacccgct gaaactgggc caggggccgg
    22441 ggctgatgct gtttgacaag cgctacttcc ccaaccagaa actggtggcg gcgttaaaaa
    22501 gctgtgccgc acataacgat ttaccgctgc aattttccac catgaaaacc ggtgcgacgg
    22561 atggcgggcg ctacaacgtg atgggcggcg ggcgtccggt tgttgcgctg tgcctgccaa
    22621 cccgctatct gcacgctaac agcggcatga tttcaaaagc cgattacgat gctctgctca
    22681 cgttgatacg ggattttctg accaccttaa ccgcggagaa agtcaacgcg tttagccagt
    22741 tccgtcaggt ggattaatgc taaacgaacg ccagttaaag attgtcgatc tgctggagca
    22801 acagccgcgc acgcctggtg agctggcgca acagactggc gtttcaggca ggaccatcct
    22861 gcgtgatatt gactatctca actttaccct taacggcaaa gcccgcattt tcgccagtgg
    22921 cagtgcgggc tatcagctgg aaatcttcga gcgccgcagc ttttttcagt tgctgcaaaa
    22981 gcacgataac gacgatcggc tgctggcgct gttattactg aataccttta ctccccgtgc
    23041 gcaactcgcc tcggcgctta atttgccaga aacctgggta gcagagcgtc tgccccggtt
    23101 aaaacagcgt tatgaacgca cttgttgcct ggccagtcgc cccggtttgg gccatttcat
    23161 tgatgagaca gaagagaaac gcgttatctt gctggcgaac ttgctgcgca aagatccgtt
    23221 tttaattccg ctggcgggcg taacacgaga caattttcag catttatcca cggcctgcga
    23281 caaccaacac cgctggccgc tcatgcaggg tgattatctc tccagcctga ttctggcgat
    23341 ttacgccctg cgtaatcaac tgaccgatga gtggccgcaa tatcccggta acgagataaa
    23401 acaaatcgtt gaacagagcg gcatgtttct tggtgataac gctgtaagaa ccctgacagg
    23461 tttgatagag aaacagcatc agcaagcgca ggtaatttca gccgatcatg tgctggggtt
    23521 gctgcaaagg gtgccaggca tcgcgtcatt gaatattatt gatacgcagc tggttgagaa
    23581 tattaccggg catttattac gttgccttgc cgcaccggtg tggattgccg agcaccgcca
    23641 aagcagcatg aataacctga aagccgcctg gcctgcggcc tttgatatga gtctgcactt
    23701 tattacgcta ctgcgtgaac agctcgatat tccccttttc gatagcgatc tgatcggttt
    23761 gtattttgcc tgtgcgctgg aacggcatca aaacgaacgc cagccgatca ttttgctctc
    23821 ggaccagaac gcgattgcca ctattaatca gctcgccatt gagcgcgatg ttttacattg
    23881 tcgggtgatt attgcccgta gcttaagcga gcttattgcc attcgcgaag agattgagcc
    23941 gttattgatc attaacaaca gccattattt actggatgac gcggtaaata attacatcac
    24001 cgtaaaaaat atcattacgg ctgccgatat cgaacaaata aaacactttc tggcgacggc
    24061 atttattcgc caacagccgg aacgcttttt ctctgccccc ggaagttttc attattcgaa
    24121 tgtacgcggt gaaagctggc aacatattac ccggcaaatt tgtgcgcaat tagtggcaca
    24181 acaccatatt accgccgatg aagcgcaacg gatcatcgcc cgcgaaggcg aaggtgaaaa
    24241 cctgattgtt aatcgcctcg ccatcccaca ttgctggagc gaacaggagc gacgttttcg
    24301 tggatttttt attaccctcg cccaaccagt tgaggtgaat aacgaagtca ttaaccatgt
    24361 cttgatcgcc tgcgccgccg ccgatgcgcg tcatgagcta aaaatattta gctatctggc
    24421 aagcgtattg tgtcagcatc cggcagaagt tattgccggg ttaacaggat atgaggcatt
    24481 tatggagtta cttc
//
LOCUS       DAZOYL010000027        24372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZOYL010000027 DAZOYL010000000
VERSION     DAZOYL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.027"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            259..396
                     /locus_tag="ACHUOY_000813"
                     /pseudo
     CDS             259..396
                     /locus_tag="ACHUOY_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(504..1739)
                     /locus_tag="ACHUOY_000814"
     CDS             complement(504..1739)
                     /locus_tag="ACHUOY_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0681673.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1886..1962)
                     /locus_tag="ACHUOY_000815"
     tRNA            complement(1886..1962)
                     /locus_tag="ACHUOY_000815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1926..1928),aa:Pro,seq:tgg)
     gene            complement(2005..2091)
                     /locus_tag="ACHUOY_000816"
     tRNA            complement(2005..2091)
                     /locus_tag="ACHUOY_000816"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2055..2057),aa:Leu,seq:cag)
     gene            complement(2112..2187)
                     /locus_tag="ACHUOY_000817"
     tRNA            complement(2112..2187)
                     /locus_tag="ACHUOY_000817"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:His,seq:gtg)
     gene            complement(2246..2322)
                     /locus_tag="ACHUOY_000818"
     tRNA            complement(2246..2322)
                     /locus_tag="ACHUOY_000818"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2286..2288),aa:Arg,seq:ccg)
     gene            complement(2423..3808)
                     /locus_tag="ACHUOY_000819"
     CDS             complement(2423..3808)
                     /locus_tag="ACHUOY_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0681674.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3999..4739)
                     /gene="wecG"
                     /locus_tag="ACHUOY_000820"
     CDS             complement(3999..4739)
                     /gene="wecG"
                     /locus_tag="ACHUOY_000820"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR0681675.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4742..6094)
                     /gene="wzyE"
                     /locus_tag="ACHUOY_000821"
     CDS             complement(4742..6094)
                     /gene="wzyE"
                     /locus_tag="ACHUOY_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR0681676.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6091..7170)
                     /gene="rffT"
                     /locus_tag="ACHUOY_000822"
                     /gene_synonym="wecF"
     CDS             complement(6091..7170)
                     /gene="rffT"
                     /locus_tag="ACHUOY_000822"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR0681677.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7167..8417)
                     /gene="wzxE"
                     /locus_tag="ACHUOY_000823"
     CDS             complement(7167..8417)
                     /gene="wzxE"
                     /locus_tag="ACHUOY_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR0681678.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8419..9549)
                     /gene="rffA"
                     /locus_tag="ACHUOY_000824"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8419..9549)
                     /gene="rffA"
                     /locus_tag="ACHUOY_000824"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR0681679.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9554..10228)
                     /gene="rffC"
                     /locus_tag="ACHUOY_000825"
                     /gene_synonym="wecD"
     CDS             complement(9554..10228)
                     /gene="rffC"
                     /locus_tag="ACHUOY_000825"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR0681680.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10206..11087)
                     /gene="rfbA"
                     /locus_tag="ACHUOY_000826"
     CDS             complement(10206..11087)
                     /gene="rfbA"
                     /locus_tag="ACHUOY_000826"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0681681.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11106..12173)
                     /gene="rffG"
                     /locus_tag="ACHUOY_000827"
     CDS             complement(11106..12173)
                     /gene="rffG"
                     /locus_tag="ACHUOY_000827"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0681682.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12173..13435)
                     /gene="wecC"
                     /locus_tag="ACHUOY_000828"
     CDS             complement(12173..13435)
                     /gene="wecC"
                     /locus_tag="ACHUOY_000828"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR0681683.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13432..14562)
                     /gene="wecB"
                     /locus_tag="ACHUOY_000829"
     CDS             complement(13432..14562)
                     /gene="wecB"
                     /locus_tag="ACHUOY_000829"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR0681684.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14618..15664)
                     /gene="wzzE"
                     /locus_tag="ACHUOY_000830"
     CDS             complement(14618..15664)
                     /gene="wzzE"
                     /locus_tag="ACHUOY_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR0681685.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15676..16779)
                     /gene="wecA"
                     /locus_tag="ACHUOY_000831"
     CDS             complement(15676..16779)
                     /gene="wecA"
                     /locus_tag="ACHUOY_000831"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR0681686.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(17019..18278)
                     /gene="rho"
                     /locus_tag="ACHUOY_000832"
     CDS             complement(17019..18278)
                     /gene="rho"
                     /locus_tag="ACHUOY_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR0681687.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18363..18464)
                     /gene="rhoL"
                     /locus_tag="ACHUOY_000833"
     CDS             complement(18363..18464)
                     /gene="rhoL"
                     /locus_tag="ACHUOY_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR0681688.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18605..18934)
                     /gene="trxA"
                     /locus_tag="ACHUOY_000834"
     CDS             complement(18605..18934)
                     /gene="trxA"
                     /locus_tag="ACHUOY_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR0681689.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19065..20330
                     /gene="rhlB"
                     /locus_tag="ACHUOY_000835"
     CDS             19065..20330
                     /gene="rhlB"
                     /locus_tag="ACHUOY_000835"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR0681690.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20464..21948
                     /gene="gppA"
                     /locus_tag="ACHUOY_000836"
     CDS             20464..21948
                     /gene="gppA"
                     /locus_tag="ACHUOY_000836"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR0681691.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21995..24016)
                     /gene="rep"
                     /locus_tag="ACHUOY_000837"
     CDS             complement(21995..24016)
                     /gene="rep"
                     /locus_tag="ACHUOY_000837"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR0681692.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24233..>24372
                     /locus_tag="ACHUOY_000838"
     CDS             24233..>24372
                     /locus_tag="ACHUOY_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681693.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         6142 a         6671 c         6008 g         5551 t
ORIGIN      
        1 ataacacata tactaattgc cgttaaaact aaaaaagcat taataatcaa cgcaatataa
       61 taaacccgcc ttacatatca actgcgccag aggtaggatt gaaaacgctc tcctgatttt
      121 ccaattcatt ttctggataa ataaataatt tatttttgtc actattattt atgtaatcat
      181 cctgtcaggg agagggatct caattatcaa tgcttaatta cgtcatcatt ttgatgacat
      241 gaaaccggag cattttgcat ggaattttcg ttttcaccca aacttcttgt tgttgctgtc
      301 gccgccgctc ttcctctcat ggccagcgca gcagataccc cgtcaactgc caccgcacgc
      361 aaaggctttg ccggatacga tcacccaaac cagtatccac agaacgctgc aatttattga
      421 atttgcacga tcatgtaggc cggataaggc gtttacgccg catccggcaa tcaaccgcag
      481 gcggccacaa aatggccgct tttttactta ttcaccacca gcaaatgtgc ctgcataatg
      541 tcactggccg ggcgaccgtg cgccagcaaa tcagccattg ctttaagata tggcggtaga
      601 tggcggaaat aacgctgata cccggcacac aaataattca gccccggttt gccgctggca
      661 tcgagcatga agcggtgttt cgggcagcct ccccagcacg cttttaacac gttacaactg
      721 cgacactgcg ccggtaattg cttaaattta tcttcaccaa acacctgctg ttgcggggaa
      781 tcgatcattt ctgcaattgt ttgctgatgc atattcccca gccgatattg cggataaaca
      841 tagtgatcgc aggcgtaaac gtcgccgttg tgctcaacaa tcaccgagcg cccacaggtt
      901 ggctgatgat ggcaaaccgc acccggcgca ccgacaaaat tggcaaacgc ccattcgata
      961 ttcatcacga aaatcttgcc gacgtcgcgt ttgatccagt ggtcgaatat cgccaccaga
     1021 aattcaccaa attcatcggg gcgcaccgac cattccgtca gttccccctg aatatcacca
     1081 ggcgcatgca atttcagtcc atcgcgggca gctgtttcat cagccagacg ctcgaccacc
     1141 ggaataaact ggatgaattc gactcccgca tcgcacaaaa aatcataaac ctgcaacggt
     1201 tgctgcgcgc tggtgcggtt gacgcagacc agcacgttat agtcgacatg atgtttttgc
     1261 agcagcgtca gggcacgcat caccagctta tgcgttggtc tgccaccttt ggtcacgcga
     1321 tattgattgt ggatctcagc cggaccgtcc agcgataacc caacaagaaa atgattttct
     1381 gccagaaatg cacaccattt atcatcgagc agcacgccgt tagtctggaa gctgttactt
     1441 atcttcctgc cagcaccgta tttcgcctgt aactttacgg cacggcggta aaaatccagc
     1501 ccgagtaacg ttggttcgcc gccctgccag gtaaaagcca cttcgttttg cggttcgctg
     1561 gcagcgatat agtgacggac atacgcttcc agcgtgtcat cgtccatatg cgtgactggc
     1621 ttttcgcggt aaagggattg tttttcgaga taaaaacagt agtcacagtt cagattacaa
     1681 tcggaaccac tcggtttcgc catcacatga aaagcacgcg ttggaacctg ttgcagcatg
     1741 gtcactcctt gcctgaagtc atccttcaat acgttaaggg cgaaatcata ccgtggggca
     1801 atgagttatt acgggcgttt attgcgaagg gagggtgacg aaatgcacag aaaacaaaaa
     1861 accccgccga agcggggttc aaaattggtc ggcgagagag gattcgaacc tccgacccac
     1921 tggtcccaaa ccagttgcgc taccaagctg cgctactcgc cgaaatactg ctttttgaat
     1981 ttttagttca attctttaaa gtcgtggtgc gagggggggg acttgaaccc ccacgtccgt
     2041 aaggacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg cattgtcaca
     2101 acttctaata atggggtggc taatgggatt cgaacccacg acaactggaa tcacaatcca
     2161 gggctctacc aactgagcta tagccaccac tacaaatctt gttacgcggt attactacca
     2221 ccgcagctca agcgccggga ctaaatggcg cgcccgacag gattcgaacc tgagacctct
     2281 gcctccggag ggcagcgctc tatccagctg agctacgggc gcttagcgcc gttgcggggg
     2341 gataatacgg acttcccacc cctctgtcta gtcccttttt aaataaattg cgcgtttggt
     2401 tacggtttgt gcgttttgct gcttattcct ccagtttatg cgctttcccg tggcgattaa
     2461 ggccaaaaac tttataaatc gccgtcaccg ccagcataaa gatgatgcca acaaacagcg
     2521 acatacgcgt atcttcatta aagtacatgc cgatcaaaac gcaaatcagg aatgccattg
     2581 ttacgtagtt ggcccacggg aacaggattg agcggaacgg atggctggca atcgccgctt
     2641 tatgtgcacg ccgaaaacgc agctggctta tcaatatcac aaaccatggc accatccccg
     2701 gaagcacgct ggcactgtag acgtagacaa acacacgctg cggattggga atgatgtagt
     2761 tcaggcatga gccaattagc agaatagcaa tagatactgc cacacccgca actggtacgc
     2821 cgtgacggga aactttcgcc atcgccgccg gtaactgacg gtttttcgcc agtgcgtaga
     2881 gcatacgtcc gcaactgtac atgccgctgt tacagccaga gagcgcagcc gtcagcacca
     2941 caaagttgat aatgcccgcc gctgcggtaa taccgatttt ggcaaaagtc agtacgaacg
     3001 ggctgccgtt gctgccaatt tcattccacg ggaagatggt gacgataacg aaaatcgcgc
     3061 ctacgtagaa aatcaggatc cgccacagca ctttgcctac agcactgcgc agcgtcacct
     3121 gcggattctt cgcttcaccg gcagtaatgc caatcagctc cacgccctgg taggacgcca
     3181 ccacaataca cagcgcggtc aggaaccctt tccagccacc cgcaaagaaa ccgccatgct
     3241 ctgtgagatt gctaaaacca atcgactgcc cgccattgcc aaagccaaag aaaatcacgc
     3301 ccaggccaat gacaatcatc acgataatcg tggtgacttt gatcatcgcg aaccagaact
     3361 cgatttcgcc gtacaaccgc accgccgcca gattcgccaa cgccaccagc gccaccgcga
     3421 tcaatgcagg tatccactgc gccatctccg ggaaccagaa ctggacataa acgccaatgg
     3481 cggtgatttc tgagatcccc accgccatcc acataaacca gtaagaccag gcggtgagat
     3541 aaccaaaaaa cgggctcata taacgatgcg cataaacggc gaacgaaccg gtaacaggtt
     3601 caaggaacaa catttcgccc attgaacgca tgatgaaaaa gacgaacagc ccggcgatga
     3661 tataggccaa caatacggat ggcccggccc atttcagggt actggcggcc cccataaaca
     3721 ggccgacgcc aatagtgccc cccagggcga tgagttcgat atgtcgagct tccagcccac
     3781 gctgtagctc tggtttgtta tctgccataa atcctcgtgt tgtgtttgca tgctttccgg
     3841 tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa tgttttcgta attcaggaga
     3901 aatggcaaat aaagcattaa aaatttgaat gctttgtgta ataaaaaagc agacaggcga
     3961 cggagtgacc actccgtcgc tttacaaaga gaggaaaatc agaggttgcc ggtgtagtgc
     4021 cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc ggctcggctg cgaaagcagg
     4081 cggtagagcc actccagtcc cagcgtttgc cagattttcg gtgcgcgttt tacgtgaccg
     4141 gtgaaaacat cgtaagtccc gccaacgccc atatacagcg catctggatg caccagacgg
     4201 cagtcgcgca tgaagatctc ctgctttggc gatcccatcg caacggtgac gatttgcgct
     4261 ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct cgggtttaaa ataaccatcc
     4321 tgactgccaa cgatattcac attccattgg ttgcgcagtt tagcttcagt ttgcgccagc
     4381 acttcaggtt taccgcccac aagaaatacc ggcgtccctt ctttgcctgc gcgcgccatc
     4441 agctcttccc agagatcggc accggcaacg cgggaaacct gcgcctgcgg gtacttttta
     4501 cgtactgaac gtacaacgct gatgccatcc gcatatttaa attcggcagc gttaattaac
     4561 tccctgacct cggcgttatc ttcaatggtc agcatttttt cagcattaat ggcaaccagc
     4621 gttccctgct taagctgccc gtcagcaaac agataatcga gggcgtgctg catatcacgc
     4681 caaccaatca actgtaagcc acgcagcgta taggtcggtg ccgtggtgtt gttattcatt
     4741 gttatccttc aacctgcgtc cggagcgatg attttgtacg tttatgaatg agtccggcgc
     4801 tttcaaaaag ccagtacagc agttttgcga tcatcagaca tgcgccgaag accacgataa
     4861 aaaagactac gcgtgagaca aacgaatcca gcccttcacg cgccagcacg atcatattga
     4921 agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt atagcgatta ggctcgcggt
     4981 tgcccagctc atacagccag tcgaaccatt tgatgatcag accaaccacg atcgccccga
     5041 gcgggatgaa caacgcgccg cccatcacca ccagtgagcc tataagcgta ggcgagatcg
     5101 ccagtccgga gtggttattc agcacttccc aggtaaaata gttggctgag ttcagcacca
     5161 tactcgggcg acccggccac agccaggaag ggataaagac atagaaatcg cggacaattg
     5221 gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa cagcaacgcc agattctccc
     5281 acggggagaa ggtgtcgcga gtgagataga gaaacgtata gaacgcttca tcgccgctca
     5341 cattcattcc atagcgtttt agcgccagcc agaacatgcc aacaatcccc agcacgcccg
     5401 ccgccgccag catccacaac gaaatccagc cgcgaataat gccaataaac aggaagatag
     5461 cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat catataagtc agcaagccaa
     5521 aggcgaccgt gctgacgagg aaaaacagcc acgctttgct gtcctggcgc agaaagtaga
     5581 ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt taacgccacg ccggagactt
     5641 cactggagaa gatctgactg taggagttaa gccggaacag caaaaagccg ttgtgcatga
     5701 agaagatgcc gacgcttacc agcgcgatac ccatcaggat cacccacgta agattggtct
     5761 ccacgcggtt catggtaaac agcggacggc gcggtacatc agcaacgcgt ttgcgtaggc
     5821 gggttttgta ggtgacatag taaaccgcgt agaagcagcc cgcagaaagc aacgcctgca
     5881 acaagatttc tggcggcgcg acaccaacat caaagcgaaa taccagcacg ctggtcagcg
     5941 ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa gaagacattg aagttaaagc
     6001 gcacacggcg aaactcaaac caggtcagcg tggcaataaa cagcgtgcag agcagccaga
     6061 ccacaaacag gccactgaat tgcagcagac tcatgcgacc tccccggcgg caatagccaa
     6121 cgcccgctgc cagccttgta gatagttagg gctaaagaag gcaatggtgt ttttatccac
     6181 cgacgccaac tggcgctgcg cttcacgcac aatatcctcg ttgagatcgt cggtagtaaa
     6241 cagcaccggc aaatgctgtt ccgtcatatc ctgccagaac ggattttccc ggttaagcac
     6301 acaaggaatg cccgcctgaa tcagtaagca aagcgtacca atgccctgct ggcgggcaaa
     6361 aataaagtaa ccaagatcgc actgacgaag tagcgccaga taggcgtcaa attccagttt
     6421 ttcgctcaaa atttgcagat tttcttcgct gaataactcc agccccgcct gacgaacttc
     6481 ctcaatgtac gcttcgttat taggcggata tcccatcggc accaccactt ttaccgtatc
     6541 gccaaattgc tgatgaacgg cgcgcaaggc agcaatatgg tcattgctgc ggtcaccgga
     6601 gttccccacc agaatggtca ttttcccttc acgttgccga tcattcgcca tcgtattgag
     6661 agaagggtcc atccgcgtcg ggaagtacag cagttcgccc cgcacctttg gatgcgtttt
     6721 ggcaaaaaag ctcaaatcac cgcgggtggc aaatacacag ccgactcgct tttgcgccag
     6781 gcgacgtagc gggtaaaaaa gcttatatct caagccactg gaaagctcat acaggtctgc
     6841 cccccagata tgccagaaaa actggctggg cttaatgcca ccactcagca gagccagcca
     6901 cagtgtggga ttgaactgac cgtggaaaaa aaatcgctgc tgacggttag cttttgcttt
     6961 cgcgatgacc gcttccgcca gcgatttttt cccagggaaa aattgcacag aaagcgccgg
     7021 acagctatca cttaagccgt cgtccttgcc aacaaccata aactcgcgcg cgtgctcgct
     7081 cgtcgcggcc agcgcgtcat tgaaaaaccg caaaacggtt cggttatggt gagggatatc
     7141 cgatcccagt acgtgaatca gtacagtcat gcccgcctac gccagagtaa aaacacgcca
     7201 caacaaagag aaaaatagac gatataagtt gccatatatg cctgcgccgc gcccagtgca
     7261 ccatgcgcag ggattagcca gtgggcaaat accatcaata aagtgaactg gctgacttcc
     7321 gccagaatat aaaaccgcag tgacgctttg gcgatcacca gataaccaaa gacataagcg
     7381 cccactttta acacatcacc cactaactgc caggcaaaga gatcgcgcat agcggtaaat
     7441 ttattcgaca acagcaacca gatagcaaaa tcacgcagca accagacggt aaaactcgcc
     7501 gccgccactg ccggtaagac gaatttcagc gatttaacca cttcccgggt gatatcgcgc
     7561 ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc tgaacgatgc cgtaataaat
     7621 tgcaggtagg catcggaaat actgctcacc ccttgccaga tccccacttc atcccagcta
     7681 tactgcgccg ccagcagttt acgcatcatg atgtaagcaa caggcaaggt caccgacgta
     7741 atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca gaccgttatc ccagctgggt
     7801 ttcagatagc ttaacgggat gacaccacgt ttgatcaaca tgatggcggc aggaatcacc
     7861 accagcgcgg gaatcagcgc cagacccagc aacgcccctt cataaccgcc caaacggtaa
     7921 ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa tcagcgataa cgcattacct
     7981 gcggcatcgc gaaagccttt catcagcgcc agtaacaggt tgccccaggc gatccccatt
     8041 tgcaccagcg ccactaaacg caccagcccc tgatagtcgg tattaccaaa cagtccctgg
     8101 ctgattggcg cagctgccag cacaaaaacc agcgccatca gcgtagagaa accaagtacc
     8161 atcgctgatg aagtgccgac cacgcggcgc agctgttgcg gattatcatg gtactgggca
     8221 acgtatttgg ttacaccgtt aaagatgcca gctccggcaa gcacgccgag cacggtaatc
     8281 aactggcgga aatttgccgc cagcccaagc cccgccggac caaatgacac agccagcaac
     8341 ttaccgacca gtaacccggc accaatcttg accagtgtac tggccgccgt ccacaaggac
     8401 gcttttgcca acgacatatc aggagaagta gttcaacaaa gtcgcaatta ccgtacgctg
     8461 attgacgggc gacaggttgt agaacagcgg caggcgcagc aggcgctcgc tctctttggt
     8521 ggtgtagcga tcttcaccgt ggaactcacc aaagcgttcc cccgcagggc aaccgtgcag
     8581 cggaatgtaa tggaacaccg ccatgatttc cgcttctttc agaaagttaa tcaacgcgct
     8641 ccggtcatca atatcccgca gtttaatgta gaacatatgc gcgttctgca cgcagccatc
     8701 gggaatcgac ggcagctcga tacgcccggc tttcgccaga ggcgctaacg catcgtagta
     8761 gttttgccac agcgccagac gttgctggtt gatacgatcc gctgcttcca gttgcgccca
     8821 caggtacgca gcttgcagat cggacatcaa atagctggag ccaatatcgc gccaggtata
     8881 tttatcgacc tgaccacgga agaactggct gcggttagtg cccttttcac ggatgatctc
     8941 ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg ccgccttcac cacccgccgt
     9001 atagtttttg gtttcatgga agctaaagca gccaatatga ccaatggttc ccagtgcacg
     9061 ccctttgtaa gtggacatca cgccctgagc ggcatcttct accacaaaca gattatgctt
     9121 tttcgccaac gccataatgg tgtccatttc gcaggccaca cccgcgtaat ggaccggcac
     9181 gataacgcgc gttttgtcgg tgatcgccgc ttcaatcagc gtttcgtcga tgttcatggt
     9241 gtccgggcga acatccacaa aaacgatttt tgcgccacgc agcacaaagg cattggcggt
     9301 ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca ggctggatat cgagcagcag
     9361 cgccgccatc tccagcgaag cggtgcagga cggcgtcagt aacactttgg cgctgccaaa
     9421 acgttgctcc agccactgct ggcagcgacg ggtaaaaccg ccatcgccac acagtttgcc
     9481 gctacccatt gccgactgca tatagtcgag ttcggttccc accaccggcg gtgcgttaaa
     9541 tggaatcatg tgatcacctg tataaccagt acgcggtgct ttctacattc gcaccacttt
     9601 gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt cgccacccgc aaagttgttt
     9661 taccgcgagc atacgcccag tttagcgccg tttgcatcag ctcagcacct gcaccgcgtc
     9721 cagccagcag gccaattcgc gcatctgtcg cattgagttc ccgtaaagag acataaccgc
     9781 gaataccgcc ggacgccgca cgtaaaatca gacattgatg atcaaaggtg ccgcgcacgg
     9841 cattttcaat ccactgtgca taaaagcgtc cgctggcgtc aggcgcatac cacggcgcac
     9901 gaaaacggct ttgcgcaaat gcggcgctgg ctaactgacg taatgcggga atatcggtct
     9961 cttgtgccac tacagcacca ctatcactga cattgttcac gggtagtgcc aaatcaactt
    10021 caccttctac cagggagaat cccagctgtt gcagggcatc cagttcaccc gtatttgatg
    10081 ccgcaatttt ggcctgcacc cgtgaccacg gcgctaacac gtctggcgtc aggagctgtg
    10141 cttcagacgt aatgcgcacg atggcgctgt taacaccaaa gaaggcgttt tcccaggtta
    10201 gtggctcaat actggcgcgg acgggcacga agtaactcca gcagatattg gccgtagcca
    10261 gttttcgcta atgaactggc agcacgcttc acaccctcgt catcgagcca gccgttacgc
    10321 caggcgatct cttccaggca ggcaatctta aagccctggc gtttttctac tgtctgcaca
    10381 aaggtactgg cttcaatcag gctgtcgtga gtgccagtgt ccagccaggc aaatccgcgc
    10441 ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc
    10501 tccagttcac cacgctccga cggcttcacc tgctttgcgt actccacgac tttactgtcg
    10561 tagaaataaa gcccggtcac cgcccagttt gacttcggct gttttggctt ttcttccagc
    10621 gagatagcgc ggaaattgtc gtcaaactcc accacgccaa agcgttccgg gtccatcacc
    10681 tgatagccaa ataccgtcgc cccttccgtg cgcgccgcaa catgacgcag cttcggactg
    10741 aagccctgac cgaagaagat gttatcgccc agcaccagac aagaaggttc accgttgagg
    10801 aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg ggctgggctg ttcggcgtat
    10861 tccagctgga taccgaactc actaccatcg cccagcaggc gctggaaata acctttatct
    10921 tccggcgtag tgatgatgag aatttcgcgg atatcggcca acatcagcac cgacagcgga
    10981 tagtaaatca ttggcttatc gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc
    11041 ggatgcaatc gggtgccgga accgcccgcc aggataatac ctttcatgtt tgcctccgct
    11101 gaaaattagc ctttcagacc taaacgctcg ccctgatagc tgccgtcctg cacctgcttc
    11161 caccagcttt cattagccag ataccactgc accgttttac gcattccact ttcaaaggtt
    11221 tcctgtggca gccagccaag ttcacgggca attttcgaag catcaatggc ataacgcaga
    11281 tcatgccccg gacggtcagc gacaaaggtg atcaagtcac gatagtgcac cacgccgtgc
    11341 ggcttgttcg gagccagttc ttccagcagc tcgcaaatgg tttccacaac atcgagattc
    11401 ttacgctcgt tgtgaccacc aatattatag gtttcaccga ctttcccggt ggtcgccacg
    11461 caatacagcg cgcgggcgtg atcttccaca tacagccagt cacggatttg ctgcccgttg
    11521 ccatataccg gcagcggttt acccgccagc gcgttgagga tcatcagcgg gatcagtttt
    11581 tccggaaagt ggtaaggacc gtagttattc gagcagttgg tgataagcgt cggcagaccg
    11641 taggtccgca gccaggcgcg caccagatgg tcgctgctgg cttttgacgc ggaataaggg
    11701 ctgctcggcg catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca
    11761 tatacttcgt cggtggagat atgatgaaaa cggaacgctg attttttatc ttccgtcagc
    11821 gtattccagt aagcccgcgc cgcttcaagc aatgtataag tcccgacaat gttggtttca
    11881 ataaacgctg ccgggccgtc aatagaacgg tcaacatggc tttcggctgc cagatgcatg
    11941 acacagtctg gctgatgctc agtgaatacg cgtgccagtt ctgcccgatc gcagatatca
    12001 actttctcaa aggcaaagcg ctcgctttgc gcgaccggtg ccagcgacat caggtttccg
    12061 gcgtaggtca gcttatcgac cactaccacc gcgtcgctcg tttcgttgat gatataacgc
    12121 accagcgccg agccaataaa cccggcacca cctgttatca gaatttttct catcagcgcc
    12181 agactccttt ggcatcgacg acatactgct gatggacatt gtcgccattg ataactttaa
    12241 actgactatg atcgaccagc atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct
    12301 gcgccagagt acaaagcccg gtcagttttt tcggcaactg gtggatgtta ggctcaacaa
    12361 ccagagtttc gccgctatgc cactgggcga tcagttcagc gatttccatc gccgggcttt
    12421 cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac
    12481 tggcgcgttt atcggtagcc gccaggcaat cagccaccgc cgctttcacc tgatcgataa
    12541 cccagaacgg tttgtgatcg ttcacttcgc gcgcggtacg gataagccgc gcctgctggg
    12601 ggttctgtgc cacgataaac cacggatcaa cagcaatgca gtgaccgccc acgccagggc
    12661 caggctgaag aatattaacg cgagggtgac gattcgccag gcgaatcagt tcccagacgt
    12721 taatcccctg atcggcacaa atcagcgaca attcattagc aaaagcgata ttcacatcgc
    12781 ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg cgagttagtg acgacacact
    12841 caccttcgag gaaaattttg tacagttcgc tggcgcgggt cgaacaaacc ggcgtcatac
    12901 caccaatcac gcgatcgttt ttaatcagct cgaccataac ctggcccggt aacacgcgtt
    12961 ccgggcagta agcaatgttg acgtccgcct gctcgcccac ctgctgtggg aaagtgagat
    13021 ccggacgcat ctctgctaac cattctgcca tcttctcggt tgaccccacc ggcgaggtgg
    13081 attcaaggat caccagcgcg ccttttttca gcactggcgc aatggagcga gcagccgatt
    13141 caacgtaggt catatctggc tcatgatcgc ctttaaacgg cgtgggtaca gcaatcagcc
    13201 aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt
    13261 ttactacgct cgccaaatca ggctcgacga tatggatttc gccacgattg atggtatcaa
    13321 ccgcgtgttg gttgatatcg acaccaatca cctgtttttg ccgtgaggca aacgctgctg
    13381 ctgtaggcag cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa ctcatagtga
    13441 tatccgatta ttttttaacg cttccagaat gcgagagcat gcctgaccat caccatacgg
    13501 gttatgggcg cggctcatag tttgatattc gttttcgtct tttaaaagac gcgtcacttc
    13561 ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc
    13621 cggacgctca gtggtatcgc gcatcaccag caccggtttc cccagcgaag gcgcttcttc
    13681 ctgaatgccg cctgagtcgg tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg
    13741 taaatactcc tggggatcga tcagaatgac atttttcaca tgccccagaa tgcgattgac
    13801 cggctctctg acgttcgggt tgagatgcac cggatagaca atctggatgt cctggtgcgt
    13861 ggtggcaatg tctgccaacg cgtggcagat ttcttcaaag ccacgaccga aactttcacg
    13921 cctgtgaccg gtcaccagaa tcatcttttt atcggggtcg ataaacgggt aatttgccgc
    13981 cagttctgaa cgcagcgtat cgctgctcat cacctggtca cgcacccata acagtgcatc
    14041 aataactgta ttaccagtaa tgaagattcg gctatccgca acgttttcac gcagcaagtt
    14101 ttgccgggaa gtttcggttg gagagaagtg atacatcgcc agatgcccgg tcaatgtacg
    14161 gttagcctct tccggccacg gcgaataaag atcgcccgtg cgcagaccag cctcaacgtg
    14221 accaacagga atacgctgat aaaacgccgc caggctggtt gccagcgtcg tcgttgtgtc
    14281 gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga ataggtttta gcccttccag
    14341 aatccgacag gttatctctg tcaggccctg tcctggctgc attatgttga gatcgtagtc
    14401 aggtacaatg gaaaagagtt tcagcacctg atcgagcatc tcccgatgct gcgcagtgac
    14461 gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg gcgccatctt
    14521 gatggcttcc gggcgcgtac caaatacagt cagtactttc acatcgattc tcttcgaata
    14581 agcggcgagc gcctttgcgc tcaccgcagc agtgttgcta tttcgagcaa cggcgggtta
    14641 atgcgacacc agccccgatc agccccccga caatgcccca cataatcatc aggaaggcac
    14701 gacgtgggct atcgcgtttt accggttctt ccggcgtacg caaatagcga taggtctgaa
    14761 aacgcggatc cagggttgga ccaacattca gggtgtttaa catggcccga ttctgatcat
    14821 agtcgagatc aaaggccgga ccgacggcct gtaaattttc cagtcgagcc tggagcattg
    14881 gacgcccaag caggaacatt tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac
    14941 tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg ttcaatactg ttcatccggc
    15001 ggtcgtagat ggctttcgcc acctcttcct gacgcttcac ctgtgctttc atctggatgg
    15061 tacgcgccgc ccatgcgcct ttcagctcat cattcagatg gctggctgca cgctggctgg
    15121 caaaagcaac atactgacgt aacaggttat tagcgtcagg cgcggtttca gcaataagct
    15181 tcacgctgtc attgaccgcg cgggtaaagt ctccggggat aaactggatg ttgttaatca
    15241 tttcatccag caacgccgca tcggctttgc tgttgcccac catccgctgt ttgtaatagt
    15301 cggtttgcag ccagaactct ctgcgggtat cccacgaggc cagttgcata acaaactctt
    15361 tataggcttc gtccatgacc gatggttggt cggcagaagc catgtttgaa cggacatcca
    15421 ggttacgcaa aaattgctgc tgcgagtaat atccccccag catattcacc gttggacgat
    15481 cggtaatcgc cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc agcgcgatta
    15541 acgcaaatgc cagccccatg ccaataatcc atagcttccc agcccacaag gtacgaaaca
    15601 acccacgaat atccagttca ttttcagcgt cttcggccgg tttcccaggc attggttgtg
    15661 tcatcacatc ctcatttatt tggttaaatt ggggctgcca ccacgatttc tacgcagtct
    15721 gcgttttacg cgcttaataa agcgagcaac tttccaggca cgcttaatgc aatatccata
    15781 gaggaagaat gctagcaaaa agagcaccag catgacccac tccgggacaa aatgagaata
    15841 ttctgccagc acgccaatgg aagcgagcag tgccgcagca agggtaatca gcacaaacgc
    15901 ctgacgggaa gtaaacccgg cacgcatgat caaatggtga atatgctgac ggtcaggaga
    15961 gaatgggctc atgcctttac gcaggcgacg gtacataatc gccaccatat ccattagcgg
    16021 aatggcgatt atccacaaag cggtaaccgg gctgatggga tgggttttgc cctgggtcgt
    16081 ttcgagcagg atccagataa cggtaaaacc aatcagcgta ctgcccgcat cacccataaa
    16141 gactttgtag cggcgaccca ggataccaag gttaagcatg atgtatggca ggatggcggc
    16201 gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc
    16261 gattgctgca aacgagacgc aggacaaccc gcccagcaag ccatcaatgc catcaaccat
    16321 gttgaacgca ttaatggccg cccagacggc aaatagcgtc aggaagtaac caaacggtcc
    16381 gagcaccatc tcccaggagc caaagatata acccaggcta ctgagataaa gtttgccgaa
    16441 caccatcata acaatgccaa cagcggcctg tatggtggca cggattttta cgctgatatc
    16501 aaaacggtca tccagcgcgc caatgaaaac aagcacaccg gcacaagcga gatagagaga
    16561 tgcatgcgga atatagtaat cgacaattcc gaacgtgaag caaatccctg cgtaaaccga
    16621 aatcccccca acgagaggta tcaatccctg gtgacgtttg cggaagtttg gtttatccac
    16681 taaaccgact ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa
    16741 aatactgatg agatcagtac tcactgtcag taaattcaca atgcatgctc tcagagaaaa
    16801 ttattagcag aagtataacc acgaagacct ttattcagaa gggaaagtcc gttccgaacc
    16861 caccagtcct gtgcaaaaat taaccatttg atatgcaaat tgcataacac gccagcaaat
    16921 tccaggaatt ttcctaacat cgccagcgcg gcattaagat tacagatata aaagcaaaac
    16981 gccacgtaaa cacgtggcgt ttttggcata agacaaattt atgagcgttt catcatttcg
    17041 aagaaatcgt cattggtctt ggtcatggcc agtttattaa tgaggaattc cattgcatcg
    17101 atttcgccca tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga
    17161 gtagtgagca gttcttcttt acgggtaccg gaacggttgt agtcgatagc cgggaagacg
    17221 cgtttttcag cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct
    17281 tcgtagataa cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc gataatagtc
    17341 aggctgccgc cctcttccac gttacgcgcc gcaccaaaga agcgtttcgg acgatgcagg
    17401 gcgttggcat ccacaccacc ggtcaacact ttacctgacg ccggaacaac ggtgttgtaa
    17461 gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa cgtctttctt gtgctcaacc
    17521 aggcgtttgg ccttctcgat caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg
    17581 tcaaaggtag aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc
    17641 ggacgttcgt cgatcagcag aaccatcagc acacaatccg ggtggttgta agcaatgctc
    17701 tgggcaatgt tctgcagcag catggtttta ccggctttcg gcggtgccac aatcagacca
    17761 cgctgaccac gaccgatagg tgatgccaga tccagtacgc gagcagttaa atcttcagta
    17821 gaaccgttac cacgttccat acgcagacga gaatttgcgt gcagcggggt taagttctca
    17881 aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc
    17941 agcgcaaaat agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca
    18001 gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg
    18061 aggtaggagc tgtctgcgga acggaggaaa ccaaatccat cctgcaatat ctccagtacg
    18121 ccatcaccaa agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg
    18181 tcctgcttac gcatgcgagc caggttttcc agccccatat tttcgccgag agtgatcagc
    18241 tcagaaaccg gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt
    18301 ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac
    18361 ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga
    18421 caagacggat tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga
    18481 agcgttggaa attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta
    18541 agttagcatg actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat
    18601 tcccttacgc caggttagcg tcgaggaact ctttcaactg acctttagac agtgcgccca
    18661 ctttggttgc cgccacttca ccgtttttga acagcagcag agtcgggata ccacggatgc
    18721 catatttcgg cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc
    18781 cctgatattc gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc
    18841 actctgccca gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac
    18901 tgtcgtcagt caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg
    18961 cgtgttggtt tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg
    19021 atacgctttc gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt
    19081 taacagaaca gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa
    19141 aaaaagggtt tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg
    19201 gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt
    19261 caacgtttca ttatcttctc tctcatcctg cgattgccga tcgcaaggtg aatcaaccgc
    19321 gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca gatccatgcc gacgcagaac
    19381 cgctggcgga agctactggc ctgaagctgg gtctggctta cggtggtgat ggctacgaca
    19441 aacagctgaa agtgctggaa agcggcgttg acattctgat tggcaccacg ggtcgtttaa
    19501 ttgactacgc caagcagaac cacattaacc tcggtgccat tcaggtagtg gttctggatg
    19561 aagccgatcg catgtacgat ctgggcttta ttaaagatat ccgctggctg ttccgccgta
    19621 tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac
    19681 gtgaactggc gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga
    19741 aaacgggcca ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt
    19801 tgctgcaaac gctgatcgaa gaagagtggc cagacagagc gattattttc gccaatacca
    19861 aacaccgttg tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt
    19921 tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg
    19981 atctggatat tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga
    20041 cgcacgtctt taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc
    20101 gtacaggtcg cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa gagtatgcat
    20161 tgaatttgcc tgctattgaa acctatattg gtcactcaat tccggtaagc aaatacaatc
    20221 cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct cacgcgccca cgtacaggca
    20281 atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc
    20341 atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg atgagcatac
    20401 tgtagtaagt gattcaggtg aaagaatgca gccaacacag agacagattg aaggatgaag
    20461 agtatgggtt ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat
    20521 atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat aaaacgcaaa
    20581 gtgcgtctgg ctgctggcct gaatagcgaa aatgccctgt ctaatgaagc aatggagcgc
    20641 ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt
    20701 cgcgttgtcg ctacggcgac attacgtctt gccgtcaatg cgggtgattt tattgccaat
    20761 gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg gtgaagagga agcacgtctg
    20821 atttatcagg gcgttgctca cacaactggc ggtgccgatc agcgcctggt ggtggatata
    20881 ggcggtgcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc gttgttcagc
    20941 ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa tctggggcag
    21001 gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa
    21061 ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag
    21121 gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaattg
    21181 aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg gctgacgctg
    21241 gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga tcgccatttt taccgaactg
    21301 aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg
    21361 ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc
    21421 tttatgatcg atattgatca ggcgcagcgc gtagccaaag ttgcggctaa cttcttcgat
    21481 caggtggaaa aggaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt
    21541 cagcttcatg aaatcggcct gagcgttgac tttaaacaag cgccgcaaca cgctgcttat
    21601 ctggtgcgta atctggatct tcccggtttt acccccgcac agaaaaaatt gctggcgacg
    21661 ctactgctca accagactaa tccggtcgat ctctcatcgc tgcatcagca aaatgccgta
    21721 ccgccgcgcg tcgcagaaca actctgccgt ttactccgcc tggccatcat ttttgccagc
    21781 cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc
    21841 ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgctcag
    21901 gaaagccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc
    21961 ggatgcgatg ctgacgcatc ttttccggcc ttgattattt ccctcgtttt gccgccatca
    22021 tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcgctgacca
    22081 ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa aagcggctcg
    22141 gctccgggcg caccagctcg ccgtactgac ggcgttcttt acacagcgta aaggtcaatt
    22201 ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg atattatctt
    22261 catcgatgct gctctggtgc ggcaaaaacc cttcttccat accgaccatg tagacataag
    22321 gaaactccag cccttttgac gcgtgcagag tcatcagttg cacctgatcc agctcttctt
    22381 cactctcacc acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc tgggtgagcg
    22441 tcatcggctc atccagttcg ctgccttcca gcatctccgt catccagcta aacagttggt
    22501 tgacgttctt catgcgcatt tcggcggctt tcgggctggg cgatgtttcg tacagccagg
    22561 attcataatc catgccgtgg atcagatcgc gcaccgcggc aatcggctcc cgctccgcca
    22621 gacgctggat ttctgccaac cagtgagtaa agcgggtcaa tgcttcataa ccacgtccgc
    22681 taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca
    22741 tcgcccactc acccagcttt ttcagcgtcg ccgggccaat ctctctcttc ggcgtgttaa
    22801 cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga taagccagca
    22861 agtccttgat ttcaggacga gagaaaaacg acgtaccacc agaaattttg tacgggatgc
    22921 ggttttgcat caggaatttt tcaaacactc gcgactgatg gttaccgcga taaagaatag
    22981 cgtaatcttt gtactgcgtt ttattgacga agtgatgcgc aatcagttca ccagtgacgc
    23041 gctcggcttc atgttcttcg ttattcgcgc ttaaaacttt aagctctgtg ccgtaaccca
    23101 gttcggagaa cagacgcttc tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg
    23161 ctttcaaaat acgtccggaa gagcggtagt tctgctcaag cttaatcacc ttcagcgccg
    23221 gaaaatcctg actcagcagc accaggtttt gtggacgtgc gccgcgccag gagtagatcg
    23281 actggtcgtc atcgcccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca
    23341 gttcatactg gctggtgttg gtgtcctgat actcatccac cagcagatag cgaattttgt
    23401 tctgccagcg ctcgcggact tcttcattgc gttgcagcag caacgtcggc aataaaatca
    23461 gatcatcgaa gtcgagaaca ttgcacgctt tcaggtgtgc atcatacagc ccataacaat
    23521 gggcaaaaat acggtcccgc tcgccaatcg cactggctgc cgcctgggac ggtgttttga
    23581 gatcgttctt ccagttagag atggtcgaaa tcaactgttg caggagaact ttgtcatctt
    23641 caatcagccc ttcggtcaac tctttgagca acgccagctg atcggtatcg tcaaacagcg
    23701 aaaagttcgc cttcatcccc agcgccgcat actcacgctt gatgatatcc agccccagcg
    23761 tgtggaaggt tgagatcatc agcccacgcg cctctttgcg acccagcgtc tgtccgacac
    23821 gctctttcat ctcgcgcgcc gccttattgg taaaggtcac cgccgcgata tgtcgcgcct
    23881 gataaccgca accgcggatc agatgggcga ttttattggt gatcacacga gttttaccgg
    23941 aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc
    24001 cggggtttag acgcataggt gtattgctca atcttcgaac ggggggagga ttgtagcaga
    24061 atcgcatttg aatgtgaccg cgtgtaagcc gctaaccgat tggctgcgga tgtttaaatc
    24121 agagagggaa gaccgaaacc ggagttcatc gtgaatccat tagacttaga aaatatcggg
    24181 tcgtaacgtt gtgttattac tttttcagag tttactaagg agaggtgtac tcgtggcgat
    24241 taatttcagt cctaaggttg gtgaaatact ggattgcaac tttggtaact atcctgttag
    24301 ccagaatgga cagttcagca ccacgtatta cgatggccgc attcctcctg aaatgataaa
    24361 aaacaggtta gt
//
LOCUS       DAZOYL010000028        24309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZOYL010000028 DAZOYL010000000
VERSION     DAZOYL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.028"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..413)
                     /locus_tag="ACHUOY_000839"
                     /pseudo
     CDS             complement(1..413)
                     /locus_tag="ACHUOY_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(519..3080)
                     /gene="mutS"
                     /locus_tag="ACHUOY_000840"
     CDS             complement(519..3080)
                     /gene="mutS"
                     /locus_tag="ACHUOY_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR0681694.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3156..3290
                     /locus_tag="ACHUOY_000841"
     CDS             3156..3290
                     /locus_tag="ACHUOY_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681695.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3367..3720
                     /locus_tag="ACHUOY_000842"
     CDS             3367..3720
                     /locus_tag="ACHUOY_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR0681696.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="ACHUOY_000843"
                     /pseudo
     CDS             complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="ACHUOY_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="ACHUOY_000844"
     CDS             complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="ACHUOY_000844"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR0681697.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="ACHUOY_000845"
     CDS             complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="ACHUOY_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR0681698.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="ACHUOY_000846"
     CDS             complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="ACHUOY_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR0681699.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="ACHUOY_000847"
     CDS             complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="ACHUOY_000847"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR0681700.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="ACHUOY_000848"
     CDS             complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="ACHUOY_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0681701.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9737..10198
                     /gene="hycA"
                     /locus_tag="ACHUOY_000849"
     CDS             9737..10198
                     /gene="hycA"
                     /locus_tag="ACHUOY_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR0681702.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10323..10934
                     /gene="hycB"
                     /locus_tag="ACHUOY_000850"
     CDS             10323..10934
                     /gene="hycB"
                     /locus_tag="ACHUOY_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR0681703.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10931..12757
                     /gene="hycC"
                     /locus_tag="ACHUOY_000851"
     CDS             10931..12757
                     /gene="hycC"
                     /locus_tag="ACHUOY_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR0681704.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCDYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12760..13683
                     /gene="hycD"
                     /locus_tag="ACHUOY_000852"
     CDS             12760..13683
                     /gene="hycD"
                     /locus_tag="ACHUOY_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR0681705.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13701..15398
                     /gene="hycE"
                     /locus_tag="ACHUOY_000853"
     CDS             13701..15398
                     /gene="hycE"
                     /locus_tag="ACHUOY_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR0681706.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15408..15950
                     /gene="hycF"
                     /locus_tag="ACHUOY_000854"
     CDS             15408..15950
                     /gene="hycF"
                     /locus_tag="ACHUOY_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR0681707.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15950..16717
                     /gene="hycG"
                     /locus_tag="ACHUOY_000855"
     CDS             15950..16717
                     /gene="hycG"
                     /locus_tag="ACHUOY_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR0681708.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16714..17124
                     /gene="hycH"
                     /locus_tag="ACHUOY_000856"
     CDS             16714..17124
                     /gene="hycH"
                     /locus_tag="ACHUOY_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR0681709.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17117..17587
                     /gene="hycI"
                     /locus_tag="ACHUOY_000857"
     CDS             17117..17587
                     /gene="hycI"
                     /locus_tag="ACHUOY_000857"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR0681710.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17612..18385
                     /locus_tag="ACHUOY_000858"
     CDS             17612..18385
                     /locus_tag="ACHUOY_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681711.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18547..18858
                     /locus_tag="ACHUOY_000859"
     CDS             18547..18858
                     /locus_tag="ACHUOY_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR0681712.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18855..19274
                     /locus_tag="ACHUOY_000860"
     CDS             18855..19274
                     /locus_tag="ACHUOY_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0681713.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="ACHUOY_000861"
     CDS             complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="ACHUOY_000861"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR0681714.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="ACHUOY_000862"
     CDS             complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="ACHUOY_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR0681715.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22538..23548
                     /gene="ascG"
                     /locus_tag="ACHUOY_000863"
     CDS             22538..23548
                     /gene="ascG"
                     /locus_tag="ACHUOY_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR0681716.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23696..24223
                     /gene="hydN"
                     /locus_tag="ACHUOY_000864"
     CDS             23696..24223
                     /gene="hydN"
                     /locus_tag="ACHUOY_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR0681717.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT         5493 a         6426 c         6606 g         5784 t
ORIGIN      
        1 atggcatatt ttatgttgtc attaatgatt tcaataatat gtggaaggtg atggaatttc
       61 agcaacagat ctattgcctc ttgttgctct gaatcattta aatcgaaaaa ccagtcacca
      121 ccgctggcaa gccacatatt tccatcgcca gtttcgaatg catccaacgc catcgcttcg
      181 tggttgcctt taaccgacgt aaaccagggt tggtttagca ggcgcagcac gttaagactc
      241 ttcggcccac gatcaatatt atcgcctgtg gaaataagta agtcggtttc agggtaaaaa
      301 gagagttgat gttaacggga ttgtaataac tgatattcac catgaatatc accaacgacc
      361 catatatggc gatagtgatg ggcattgatt ttttgatagc gtgtagatgg catggtttta
      421 ccctgtaaaa taagctttcc tattatacag ggtattttta tttgattcgt cagttgtcgt
      481 taatattccc gatagcaaaa gactatcggg aattgttatt acaccagact cttcaagcga
      541 taaatccact ccagcgcctg acgcggagtc agtgaatccg ggtcgagatt ttccagtgct
      601 tcgaccgcag gcgaagtttc ttctggcact gacagcaaag acatctgcgt accatccact
      661 tgcgtcgcgg cggcgttcgg cgaaatgctt tccagctcac gcagtttttg ccgtgcgcgc
      721 ttaataacct ctttcggtac gcccgccaga gcagcaaccg ccagaccgta gcttttgctc
      781 gccgcgccgt cctgcacgct gtgcataaag gcgatagtgt cgccgtgctc cagcgcatcg
      841 agatgtacgt tggcgacgcc ttccattttc tccggtagct gggtcagctc gaaatagtgg
      901 gtggcaaaca gcgtcaatgc cttaatctta ttcgccagat tttctgcgca cgcccacgcc
      961 agcgacagac catcgtaggt ggacgttcca cgcccgatct catccattaa caccagactg
     1021 tattcggtgg cgttatgtaa aatattggcg gtttcagtca tctccaccat aaaggttgag
     1081 cgcccggaag ccaggtcatc tgccgcgcct acgcgggtaa agatgcgatc gataggtcca
     1141 atctcgactt tttgtgccgg tacatagctg ccgatgtagg ccatcagcgc aatcagtgcg
     1201 gtctggcgca tataggtact tttaccgccc atgttcggac cggtgatgat caacatacgg
     1261 cgctgcggcg acagattcag cgggttagcg ataaatggct cattcagtac ttgttcaact
     1321 accggatggc gaccttcggt aatgcgaatg cccggtttat caatgaaggt cgggcaggtg
     1381 tagttcaggg tataggcccg ttccgccagg ttcaccagca cgtcgagttc cgccagcgcg
     1441 ctcgcgctct gttgcaacgc ttccagatgc ggcaacagca ggtcgaacaa ctcttcataa
     1501 agctgttttt ccagtgccag tgctttgcct tttgaggtga gaactttatc ttcgtactct
     1561 tttagctctg gaatgatgta gcgctcggcg tttttcagcg tctggcgacg catgtagttg
     1621 atgggtgcca gatggctttg cccacggctg atttgaatgt agtagccgtg caccgcatta
     1681 aagccaactt tcagcgtgtc caggccggta cgttcacgct cgcggacttc cagacgctcc
     1741 agataatcgg tcgcgccgtc agccagcgcg cgccactcat ccagctcttc gttatagccc
     1801 gatgcgataa caccaccgtc gcgtaccagc accggcggtg tgtcgatgat tgctcgctcc
     1861 agcagatcgc gcagctcggc aaactcgccc atcttctcac gtagcgcctg taccggtgca
     1921 ctatcgacag tttctaactg cgcacgcagc tccggcagtt gctggaaagc gtggcgcata
     1981 cgggccagat cgcgtgggcg agcagttcgt aaagccagac gtgccagaat acgttccagg
     2041 tcgccgacct gacgcagtac cggctgcaac tcggcggtga aatcctgcaa tgcgccaata
     2101 gtttgctggc gctcaagcaa cacgcgggta tcgcgcactg gcatatgcag ccagcgtttc
     2161 agcatacgac tacccatcgg cgtgacggtg cagtcgagca cagaagccag cgtattttcc
     2221 gcaccgcccg ccaggttctg agtaatttcc aggttacgac gtgtcgcggc atccataatg
     2281 atgctgtcct gctcacgttc catggtgata gaacgaatat gcggcagggt cgtgcgttgg
     2341 gtatctttcg catactgcaa cagacaaccg gcagcacaaa gtccgcgcgg cgcgttctcg
     2401 acgccaaaac cgaccagatc gcgggtgcca aattgcagat tcaactgctg gcgcgcggtg
     2461 tcgatttcaa actcccacag cgggcgacgg cgcaggccgc gacggccttc aatcagcgac
     2521 atctcggcga aatcttctgc atacagcagt tccgccggat tagtgcgttg cagctctgcc
     2581 gccatcgttt cgcggtcggc cggttcgctc aggcgaaaac gcccggagct gatatccagc
     2641 gtcgcgtagc cgaaaccttt gctgtcctgc cagatagccg ccagcaggtt gtcctgacgc
     2701 tcctgtaaca gggcttcatc gctgatggtg cctggcgtaa cgatacgcac aactttgcgc
     2761 tcaaccggac ctttgctggt cgccggatcg ccaatttgtt cgcagatggc aacggactct
     2821 ccctgattca ccagtttggc gagatagttt tccaccgcat ggtagggaat ccccgccatc
     2881 gggatcggct ctcccgccga agcaccgcgt ttggtcagtg aaatatccag cagttgcgac
     2941 gcgcgttttg cgtcgtcata aaacagttca taaaaatcac ccatccggta aaacagcagg
     3001 atctcgggat gctgggcttt cagcctgaga tactgctgca tcatgggcgt atgggcgtcg
     3061 aaattttcta ttgcactcat ggggttatgt ccggttccct gatattaaat ggggtgtgat
     3121 ggtttttatt ttcgttgtaa tcacataagg cgcacatgat accggagtta atcatttcag
     3181 gggaagaagc gaacggcgag ttcttgatgc tggttccggg aaagatgctg ttttgcagct
     3241 tttgcgtgat gcttatgaag caagtacgca aaattgcgtc ggttgaataa acctcctcct
     3301 tcagagccat agtccccttt caaattaagt tgtaatataa atacacctta agagtatgga
     3361 ggcagcatgt ccggcaagcg tatctctcgt gaaaaactga cgattaaaaa aatgatcgat
     3421 ctttatcaag cgaaatgccc gcaggcgtca gcggagccgg agcattacga ggcgttgttt
     3481 gtttacgcgc aaaagcggct ggataaatgt gtgttcggcg aagagaaacc agcctgtaag
     3541 cagtgtccgg tgcactgtta ccagcctgca aagcgtgaag agatgaaaca gattatgcgt
     3601 tgggccgggc cgagaatgct gtggcgtcat ccgatcttaa ccgtgcgtca tctcatcgat
     3661 gacaagcgtc cggtaccaga actgccggaa aaatatcgtc cgaaaaaacc tcgtgaatag
     3721 tatgccggac aggcagatct gtccggcaat ttgcagttaa atcaatgccg atttatcaat
     3781 tcccagccgc ttcatccgtg acagcagggt cgtgcgtttc agccccagac gttgcgcagc
     3841 gcctttaggc ccggcaacca cgccgttggt ttctttcagc acgcgcacaa tcaactgata
     3901 ttcatcttcg ccctcctggg cgacaaccgt tgcggcaggc ggcgtttcag gttccggtaa
     3961 aacaatatct ggcaatgaca gctgcagcac gttaccgcgt gttagcaata ccgcgcgctc
     4021 aatgacgttt tccagttcgc gtacgttacc cggccactcc atgttgctca aggtgcgcag
     4081 cgtctcggca ggaatgctgt cgatattgcg ccccagacga cgggcaattt tgaaggtaaa
     4141 ggctttcgcc agcagcggaa tatcttccgg acgctcgcgt agtggcggca ggtgaatcgg
     4201 gaatacgttc aggcggtaat agagatcgct acggaactct cggtcggcga ccattttttt
     4261 cagatcgcgg ttagtcgcgg caattaaacg tacgtccgtc tgaatgattt tgttgctgcc
     4321 gagacgttca aactcctgtt cctgcaatac acgcagcaac ttcggctgta actccagtgg
     4381 catatcgccc acttcgtcga ggaacagaga gcttttatcc gccagttcaa aacgaccaat
     4441 gcgctgggcg ctggcaccgg taaaagcccc acgctcatga ccaaacagat cgctttccag
     4501 caatccggca ggcatcgccg cgcagttcat tttgaccatg cggcgattat tacgcccact
     4561 gagattatgg atcgcgcggg caatcagctc tttacccgtg ccagtttcac cgaggatcag
     4621 cacggtactg tcactttgcg ccaccatttc aacttgttta agcacgctgt acatggcttc
     4681 gctgcggcca ataatctcgc caaattcact atcaacattg ttgagctgct cggtcagggc
     4741 gaggttttca tcaaccagcc gttctttcag acgatggatt tcctgatagg cgagggcgtt
     4801 atcgacagcg attgccacac gttcggcaat ctggcgcagc aaattcagat tggtagtggt
     4861 aaacactttc tcttcgcatt gcgccagttt cagcacgccc agcatggtgt cgccagacat
     4921 cagcggtaac aggcacaagg tttgaatctg gttgccctag gtgtcgaaca acatgcgttc
     4981 atagggggct aaatcgtccc gctcgtggag attgatcagc agcatctctt tacttttgaa
     5041 cacgcgttcg gtgagggttc cggcttcatc gacttcgctc tgttcgtggg cgggatgttg
     5101 tttatcaaga tagtgagtgg agtagatgtt gagtttgttt ttacggtggc tgcgtaagac
     5161 gatactgata tcgtcaatgt cgaaatagta atggatttct ttggcgactt cgctgaccag
     5221 ttcgtccata tccaggcggg aaagcaccgc gttggtaatg gcaaccagga tgcggaagtt
     5281 atcgcgctcc cggcataaca actcatagtc gacattgttg acgacgcggc tctggatttg
     5341 ttcggtgacg acagaaacga tctgcgtaaa tgtttgcaga cgattgaact ctttttcgct
     5401 ccagggacga tcgtcataac gaataaattc acagccacca aaaatatgcc cttccgccgc
     5461 cagtggcatc aggcaatagt gaccaaattt ggggtatagc ccacctgcga ccagctgcgg
     5521 ccaggtttca caaaattctt cgtaactgca atgcagcgta tcagggcgcg acaaaatgcg
     5581 gcgtaccgga ccgtgtgcca gaacagtttc gtcttcatat ttaatggggt gtctttttca
     5641 cgcgatgcgt aataagacgc acgttgagtc tgcgcttgcc acaacacaat agccgcgttg
     5701 tcggcgagcg cagaacgctt taccagttgc gaaagagcct cacacagcga ggccagatcg
     5761 ggctgctgca atagtgtccg agtgatgtcg aacaaccctt gctgtccgag atcactcatc
     5821 ggtgtatatg acattttgtt cttccaggaa agcaccgcaa agcggtgcaa aaggtcattg
     5881 actaactata ggagatttag aattttatta gcatatacgc ggaagaggtt cggcgtgtgg
     5941 taaatcgagg gttcgtttca caccatacag accggcaaga cgaacacctt tacgttccac
     6001 cacttcacca attagcgccg cgtctttccc cagtggatgg gaatgtaacg ctgccagcac
     6061 ttgctctgcc gcgttgcgtt caacggcgat caccagtttg ccttcgttgg caaagttaag
     6121 ggcgtccagt cccagcagtt cgcaaacgcc gcgcacggca ggtttaaccg gcagtgccac
     6181 ttctgaaatt tcaataccac aaccgcaggc tgccgcgaac tcatgaacta ccgcgttcac
     6241 accaccacgg gtggcatcac gcagcgcttt cacgccggga atgtcacgca gcgtctgaat
     6301 aagcggcgtc agtaccgcgc agtcgctgac cagttcgcca tccagcccca gctgctcacg
     6361 caggttaagg atagtcgccc cgtggtcccc gagcgtgccg ctaaccagta atacatcgcc
     6421 tgcggttagc gtctgtgcgc cccagtgaat attcgccgga attgcgccca tgccagcggt
     6481 gttgataaac agtttatctg ccgcgccgcg ctgcaccact ttagtatcgc cagtaacgat
     6541 ggcaatgcct gccgcgcgcg cggtttctgc catgctggtc actacggctt tcagtgtctc
     6601 catcggcaat ccttcttcga ggataaagcc acaggagaga tagcgcggaa tagcgccact
     6661 gaccgcaacg tcattggctg tgccgcaaat cgccagcttg ccgatattac cgccaggaaa
     6721 gaacagcggg tcaatgacgt aactgtcggt ggagaacgcc agacggtcgc cttctgctac
     6781 cagctgcgcc agatcaagac gtgcctggtc ttcctgctct gccagccacg ggttggcaaa
     6841 ggcttccata aacaggctgt tgattaattg ctgcatcgcc tggccgccgc tgccgtgggc
     6901 gagttggata ttattcacgc ttcattctcc tgctgacgat actgatacca cgcggcgcac
     6961 gctccttcgg aggagaccat cagtgcacca aatgcggttt gaggattaca ggtgttacca
     7021 aacagcgggc attgatgcgg cttacatttg cccgttaata cctcaccaca acgcgcgcgc
     7081 gggtcatcgc agacctgctg cggtgctggg cggaaatgtg cttcggcatc gaatcgttga
     7141 taatccggcg tcaggtgcac gccagaagat tcaatcacgc ctaagccgcg ccattcgctg
     7201 tcgccgttga cacagaacac atcagcaatt gcctgttgcg ccagcaggtt accggcatcc
     7261 ggtaccaccc gacgatactg attctctacc ttgctgtggg ccgctatttt ctgctccacc
     7321 agcatgacca cgccttgcag tagatcaagg ggttcgaaac cagcgaccac cagcggacga
     7381 tgaaaatcgc tggcgataaa attataggca tcagtgccga taaccatact aacgtggccc
     7441 ggcgcgagga acgcgtcgat accgttatcc ggctgttcca gcaaactgcg cagcgtcggg
     7501 ataagcgtaa tatgctggca gaagaagtaa aaattctgca catcgcgggc tttcgcctgt
     7561 tgcagggtga tggcggtggt cggcatggtg gtttcaaaac ctaagccgaa gaatacgact
     7621 ttacgggttg gattctcctg cgccagtttc aacgcatcca tcggcgagta aacgatgcgc
     7681 acatcggcac cgcgtgcttt tgcctgcaac agcgatccct gtttccccgg cacgcgcatg
     7741 gcgtcgccaa aggtacagaa gatgacttcc ggatggctgg caatctccac gcaggtgtcg
     7801 attctaccca tcggcagtac gcacaccggg caacccggac cgtggataaa ctcaacgttt
     7861 tcaggcagta actggtcgag gccgaattta aaaatggcgt gggtatgacc accgcacact
     7921 tccataatcc gcagagggcg ttcggcggtg taagagagat gtgaagcacg ttcgcgcaga
     7981 tgctcaatta actgcatcac ctgttccggc gcgcgatatt catcaacaaa acgcattatt
     8041 tttcctcgcc atacaacagc gcgccgacat ccggctcaac gtcaaacatg ttttgtaagg
     8101 cgtcgagagt gtcgcgtgct tcggcttcat taattacgct catggcaaag ccaacgtgta
     8161 ccagtaccca ctggcccacg cgcggctgac cgttttcatc gcagctgccg actaacgtta
     8221 aatcgacatc gcgctgaatg ccgcagacgt cgacttttgc ctggttgccg tcaatggtgc
     8281 ggatctggcc gggaacgcct atgcacatcg ctgtgtctcc agccagttca gccactggtc
     8341 catcccttcg ccgctggtgg cggaaataag gatgatttca atttctggat tgacttcgcg
     8401 ggcgcaggcg atgcacttct caacgtcaaa gttgagatac ggcaacaggt caactttgtt
     8461 gagcagcatc agcgaggcgg cggcaaacat atgcggatat ttcagcggtt tgtcttcacc
     8521 ttcggtaacg gaaagcaccg ccactttgtg tttttcaccg agatcgaagc tggccgggca
     8581 tacgaggttg ccaacgtttt cgataaacag aataccgtta tcgtccagtg gcagacgcgg
     8641 tgcggcgtcg gcaatcatct gtgcgtcaag atggcagcct ttaccggtgt tcacctgaat
     8701 cgctggtgtg ccggtagcgc gaatgcgtgc ggcatcgttc acggtttgct ggtcaccttc
     8761 aataaccgcg cacggaacgc tgtctttcag gcgcattagg gtttccgtca gcagggtggt
     8821 tttaccggaa ccagggctgg aaaccaggtt gagcaccagt tgcttgcggg cagcaaagcg
     8881 cgcgcggttg cgttcagcca gacggttatt tttgtccagc acgtcaattt cgacttccag
     8941 catccgacgc tggctcatgc ccggcgcgtg agtgcccgct tcgccatgac cgtaatgcag
     9001 gtcgccttct tcagtctggc tgggggtaaa ttcaggcgct ttaatgccgg tgattttcat
     9061 cttcgggcgt gccgccgggg caaatggcgc gctacgaaac gcggaatgag ggttatgttc
     9121 atcaccctcg atatacaggt tgccttcacc gcaaccgcat gttgtacaca tcgctcactc
     9181 ctggtctatt tctatccgcc gaatctgtaa accgtcgtct gccacaatct gtagcatgtc
     9241 accatgacac tgtggacagc ggcggacgcg ctgggtcagt agcgtcacat actgttggca
     9301 tgtttcacac cagcattcgg cctcttgttc ttcgaggtgc agtttacaac cttccgccac
     9361 gctgccgcgg caaaccagat caaaacaaaa ggcaagagag ctggtttcga cacaagaaaa
     9421 tgcgccaatt ttgagccaga ccccagttac gcgttttgcg ccgtgttttg cggcctgctg
     9481 ttcgatcaat tccagtgccc gttggcagag ggttatttcg tgcatatcgc ctcccattaa
     9541 ctattgccag ctacaagcaa taattgtgcc agtgttgatt atccctgcgg tgaataatgt
     9601 cgatgatgtc gaaatgacac gtcgacacgg cgacgaaatt catctttagc ttaaaaatct
     9661 atttaataac aataaattaa aagttggcat agaaaatgct taaagctggc atctctgtta
     9721 aacgggtaac ctgacaatga ctatttggga aataagcgag aaagccgatt acatcgcaca
     9781 gcggcattgt cgcctacagg accagtggca catctactgc aattcgctgg ttcaggggat
     9841 cacgttatcg aaagcgcgcc tgcatcacgc catgagctgc gcgccggaca aagaactctg
     9901 tttcgtcctt tttgaacatt ttcgtattta cgtcaccctg gcggatggct ttaacagcca
     9961 caccatcgag tattacgtcg aaacaaaaga tggcgaagac aaacagcgga ttgcgcaggc
    10021 gcaactgagc attgacggca tgattgatgg caaggtcaac atccgcgatc gcgaacaggt
    10081 tctggaacac tatctcgaaa aaatcgctgg cgtttacgac agcttataca ccgctattga
    10141 aaacaatgtg ccggtgaatt taagccaact ggtaaaggga caaagcccgg cagcatgagc
    10201 tgaggctttg cccgttttgc aggcgttacg cctgtttggg gatgggcgtg tcgatgagtg
    10261 tcgaaaatga catttcatcg gcatgttttc gtcaaaaatg acaatcacct gaggaatgcc
    10321 tggtgaatcg ttttgtaatt gttgactcca cgctctgtat cggctgccac acttgtgagg
    10381 ccgcctgttc agagacgcat cgccagcacg gcctgcaatc aatgccgcgc ctgagagtga
    10441 tgctgaatga aaaagaatct gcgccgcagc tctgtcacca ctgtgaagat gcaccctgtg
    10501 cggtggtttg cccggttaac gccatcaccc gcattgatgg ggccgtgcag ttgaatgaaa
    10561 gtctgtgcgt aagctgcaaa ctgtgcggca tcgcctgccc gtttggcgca attgaatttt
    10621 ccggcagccg tccgctggat attccggcaa acgccaatac cccgaaagcg ccaccggcac
    10681 cgcctgctcc ggcgcgtatc agtacgctgc ttgactgggt gccgggtatt cgcgcgatcg
    10741 ccgtcaaatg tgacctttgc agttttgatg aacaaggtcc ggcctgcgtg cggatgtgcc
    10801 cgactaaagc cctgcatctg gtggataaca ccgatatcgc ccgcgtcagc aaacgtaagc
    10861 gtgagctgac ctttaacacg gactttggcg atctcacctt gtttcagcag gctcaaagtg
    10921 gagaggctaa atgagcgcaa tttccctgat caatagcggc gtggcatggt ttgtcgccgc
    10981 cgctgttctg gcatttctct tttcttttca aaaagcgtta agtggctgga tagctggaat
    11041 tggcggcgcg gttggtagtc tgtatacggc agccgcgggc ttcactgtac tgactggcgc
    11101 ggttggcgtg agcggtgcgc tgtcgctggt aagctacgat gtgcaaatct ctccgcttaa
    11161 cgcgatttgg ctgattacgc tcggtctgtg cggtctgttt gtcagcctct acaacattga
    11221 ctggcatcgc cacgcgcagg tgaagtgcaa cggcttgcag atcaatatgt tgatggctgc
    11281 cgccgtctgc gccgtcatcg ccagcaacct cggcatgttc gtggtaatgg ccgaagtcat
    11341 ggccttgtgt gcggtgttcc tcaccagcaa cagcaaagag ggcaaactgt ggtttgcgct
    11401 ggggcgtctt ggcactctgc tgctggtgat tgcttgctgg ctgctgtggc agcgttatgg
    11461 cacgctggat ctgtgcctac tggatatgcg tatgcaacag ctgccgctcg gttccgatat
    11521 ctggctgctc ggtgtgattg gctttggcct gctggccggg attattccgc tgcacggctg
    11581 ggtgccgcag gcacatgcta acgcctctgc gccagctgcc gcgttgtttt ctacggtagt
    11641 catgaaaatt ggcctgctgg gcatgttaac cctgtcactg ctgggcggta atgcaccgct
    11701 gtggtggggg atcgcgctgc tggtgctcgg catgatcacc gcgttcgtcg gtggtctgta
    11761 tgcgttgatg gaacacaaca tccagcgcct gctggcttac cacaccctgg aaaatatcgg
    11821 cattatcctg ctggggctgg gcgctggcgt aacgggtatc gcgctcgaac aaccggcgct
    11881 gattgctctt ggcctggtcg gtggtctgta ccatctgctt aaccatagcc tgttcaaaag
    11941 cgtactgttc ctcggggcgg ggagcgtctg gttccgtacc ggtcatcgcg atatcgaaaa
    12001 actcggtggt attggcaaga aaatgccggt tatctccatc gccatgttag tcgggttgat
    12061 ggcaatggct gcgctgccgc cactgaacgg gtttgccggg gaatgggtta tctatcaatc
    12121 cttcttcaaa ctgagcaata gcggcgcgtt tgttgcccgt ctgctggggc cgctgctcgc
    12181 tgtgggtctg gcaattaccg gtgcgctggc ggtgatgtgt atggcgaaag tctatggcgt
    12241 cacgttcctc ggcgcgccgc gtaccaaaga agccgaaaac gccacctgtg cgccgctcct
    12301 gatgagcgta agcgtagtgg cactggcgat ttgctgcgta attggcggtg ttgctgcgcc
    12361 gtggctgctg ccgatgctct ctgctgctgt acctctgcct ctggagcctg ctaacaccac
    12421 cgtttctcaa ccaatgatca cattgctgct gattgcctgc ccgctgctgc cattcatcat
    12481 tatggcgatt tgcaaaggcg accgcctgcc gtcacgttcc cgcggtgcgg cctgggtgtg
    12541 cgactacgac catgaaaaat caatggtgat taccgctcac ggttttgcca tgccggttaa
    12601 acaggcgttt gcgccagtgc tgaaactgcg caaatggctg aatccggtgt cgctggtgcc
    12661 gggctggcag tgcgaaggta gtgcgttgct gttccgccgg atggcgctgg ttgaactggc
    12721 ggtactggtg gtgattattg tttcacgagg agcctgagaa tgagtgtttt atatccgtta
    12781 attcaggcgc tggtgttatt tgccgttgcg ccgctgctct ccggtattac ccgcgtggcg
    12841 cgcgcccgtc tgcataaccg tcgtgggccg ggtgtgttgc aggagtatcg tgacattttc
    12901 aaactgctgg gccgccagag cgttggcccg gatgcctccg gctgggtgtt ccgcctgacg
    12961 ccgtatgtga tggtgggcgt catgctgact atcgctactg cgctgccggt ggtgaccgtc
    13021 ggttctccgc tgccgcaact gggtgattta atcaccttac tgtatctctt tgccatcgcg
    13081 cgtttcttct ttgccatttc tggtctggat accggtagcc cgtttaccgc tatcggcgcg
    13141 agccgtgaag cgatgcttgg cgtgctggtc gaaccgatgc tgctgcttgg tctgtgggtt
    13201 gccgcacagg ttgccggttc caccaacatc agcaacatca ccgacaccgt ttatcactgg
    13261 ccgctgagcc agagcatccc gctggtactg gcgctttgtg cctgtgcgtt cgccaccttt
    13321 atcgaaatgg gcaaactgcc gttcgacctg gcggaagccg agcaggagct gcaggaaggc
    13381 ccgctctctg aatacagcgg cagcggcttt ggcgtcatga aatggggtat cagcctgaaa
    13441 cagctggtgg tgctgcagat gttcgtcggc gtatttatcc cgtggggaca gatggaaacc
    13501 ttcaccgccg gtgggctgct gctggcgctg gtgattgcca tcgtcaaact ggtcgtcggc
    13561 gtcctggtta tcgcgctgtt cgaaaacagc atggcccgtc tgcgtcttga tattactccg
    13621 cgcattacct ggactgggtt tggctttgca tttttagcgt tcgtctcctt gctggcggcg
    13681 tgattaaaga gagtttgagc atgtctgaag aaaaattagg tcaacattat ctcgccgcgc
    13741 tgaatgaggc atttccgggc gtcgtgctgg accacgcctg gcagaccaaa gatcagctga
    13801 ctgtcaccgt aaaggtgaac tatctgccgg aagtggtgga gtttctctac tacaagcagg
    13861 ggggctggct gtcggtgctt tttggtaacg acgaacgcaa actgaatggt cattacgccg
    13921 tttattacgt gctgtcgatg gagaagggga ctaagtgctg ggtaacggtt cgcgtcgaag
    13981 ttgatgccaa caaaccggag tatccgtccg tgacgccgcg cgttccggcg gcggtgtggg
    14041 gcgagcgcga agtgcgcgat atgtacggtt tgattccggt tggtctgccg gatgaacgcc
    14101 gtctggtgct gccggatgaa ctttatccgc tgcgtaaaga cagcatggat tatcgtcagc
    14161 gtccggcacc gaccaccgat gctgaaacct acgagttcat caacgaactg ggcgacaaga
    14221 aaaacaacgt cgtgccgatt ggtccgctgc acgtcacttc tgacgaaccg ggccacttcc
    14281 gtctgttcgt cgatggcgaa aacattatcg acgccgacta ccgtctgttc tacgttcatc
    14341 gcggcatgga aaaactggcg gaaacccgta tgggttacaa cgaagtgacc ttcctctctg
    14401 accgtgtgtg cgggatctgc ggctttgccc acagcaccgc ctacaccacg tcggtagaaa
    14461 acgcgatggg tattcaggtg ccagaacgtg cgcagatgat ccgcgccatt ctgctggagg
    14521 tggaacgcct gcactcgcat ctgctcaacc tcggtctcgc ctgccacttt accggctttg
    14581 actccggctt tatgcagttc ttccgcgtgc gtgaaacctc catgaaaatg gcagagatcc
    14641 tcaccggtgc gcgtaaaacc tacggcctga acctgatcgg cgggattcgt cgcgatctgt
    14701 tgaaagatga catgatccag acccgccagc tggcgcaaca gatgcgtcgt gaagtgcagg
    14761 agctggtgga tgtgctgctg agtacgccga acatggaaca gcgcaccgtc ggcattggtc
    14821 gtctggaccc ggaaatggcc cgcgatttca gtaacgttgg cccgatggtt cgcgccagcg
    14881 gtcacgcccg cgatactcgc gccgatcacc cgtttgtcgg ttatggcctg ctgccaatgg
    14941 aagtccacag cgagcagggc tgcgacgtta tttcccgtct gaaagtgcgt atcaacgaag
    15001 tttataccgc gctgaacatg atcgactacg gtctggataa cctgccgggc ggtccgctga
    15061 tggtggaagg ctttacctac attccgcacc gtttcgcgct gggctttgcc gaagcgccgc
    15121 gcggcgacga tatccactgg agcatgaccg gcgacaacca gaagctgtac cgctggcgct
    15181 gccgtgccgc gacctacgcg aactggccga ccctgcgcta catgctgcgc ggtaacaccg
    15241 tttccgatgc gccgctgatt atcggtagct tggacccttg ctactcctgt accgaccgca
    15301 tgaccgtggt cgatgtgcgt aagaaaaaga gcaaagtggt gccgtacaaa gaactcgagc
    15361 gttacagcat tgagcgtaaa aactcgccgc tgaaataagg aatcgccatg tttaccttta
    15421 tcaaaaaagt catcaaaacc ggcacggcga cctcgtctta tccgctggag ccgattgcgg
    15481 ttgataaaaa cttccgtggt aagccggagc agaacccgca gcagtgcatc ggctgtgcgg
    15541 cctgcgtcaa tgcctgcccg tcaaacgcct taacggttga aactgacctc gccaccggtg
    15601 agctggcgtg ggagtttaat ctcgggcgct gcatcttctg tggacgctgc gaagaagttt
    15661 gcccgacggc ggcgatcaaa ctgtcgcaag agtacgaact ggcagtgtgg aagaaagaag
    15721 acttcctgca acagtcccgc ttcgcgctgt gcaactgccg cgtctgcaat cgtcctttcg
    15781 ccgtccagaa agagatcgac tacgccattg cgctgcttaa gcacaacggc gacagccgcg
    15841 cggaaaacca ccgcgaaagc tttgagactt gcccggaatg taagcgccag aaatgcctgg
    15901 tgccgtccga ccgtattgaa ctgactcgcc atatgaaaga ggccatctga tgagcaattt
    15961 attaggcccc cgtgacgcca acggcattcc ggtcccgatg acggtggatg aatccatcgc
    16021 cagcatgaag gcgtcgttac tgaaaaaaat caaacgttct gcctacgttt atcgcgtgga
    16081 ctgcggcggc tgcaacggct gcgaaatcga aattttcgcc acgctttcgc cgctgtttga
    16141 tgcagaacgc ttcggcatta aagtcgttcc ttcaccgcgt catgcggata ttttactgtt
    16201 taccggcgcg gtcacccgtg caatgcgatc ccctgcgctg cgtgcgtggc agtccgcgcc
    16261 ggacccgaaa atttgtatct cctacggtgc ctgcggtaac agcggtggta ttttccacga
    16321 tctctactgc gtgtggggcg gtacggataa aatcgtcccg gtagatgtct acattcctgg
    16381 ttgcccgcca acgcctgccg ccacgctgta cggctttgca atggcgctcg gcctgctgga
    16441 gcagaaaatt cacgcccgtg gcccgggtga gcaggatgaa caacaggcgg aaatcctgca
    16501 tggcgatatg gtgcagccgc tgcgtgtgaa agtggatcgc gaagcacgcc gcctggcggg
    16561 ttatcgttac ggtcgtcaga ttgccgatga cttcctgaca cagttagggc agggcgaaga
    16621 acaggttgca cgctggctgg aagcggaaaa cgatccgcgt ctgaacgaga ttgtcagcca
    16681 tctgaatcat gttgttgaag aggcgcgtat ccgatgagtg aaaaggtggt gttcagtcaa
    16741 ctgagccgta aatttattga tgagaacgat gccacgcccg ccgaggcgca gcaggtggtc
    16801 tattacagcc tggcgattgg tcaccacctt ggggttatcg attgcctgga agcggcgctc
    16861 acctgcccgt gggatgaata tctggcatgg attgccactc tggaggcggg cagtgaagcc
    16921 cgccgcaaaa tggaaggcgt gccgaaatat ggtgagatcg tcatcgacat taaccatgtg
    16981 ccgatgctgg ccaacgcatt cgataaagcc cgggcagcgc aaacttcgca gcagaaggaa
    17041 tggagtacaa tgctgttaag tatgctgcat gatattcatc aggaaaacgc catctatttg
    17101 atggtgagga gactgcgtga ctgacgtttt actctgtgtt ggcaatagca tgatgggcga
    17161 tgatggcgct ggcccgctgc tggcggaaaa gtgcgccgcc gcgccgaaag gtaactgggt
    17221 ggtgattgat ggcggtagtg caccggaaaa cgacatcgtc gccatccgtg aactgcgccc
    17281 tacgcggtta ctgattgttg acgctactga tatggggcta aaccccggcg aaatccgcat
    17341 catcgacccg gatgatatcg ccgagatgtt tatgatgact acccataaca tgccgctgaa
    17401 ctatcttatc gaccagttga aagaagacat tggcgaagtg attttcctcg gcatccagcc
    17461 ggatatcgtc ggcttttact acccgatgac tcagccgatt aaagatgcgg tagaaaccgt
    17521 ttatcaacga ctggaaggct gggaagggaa tggcggcttc gcgcagttag cggtggaaga
    17581 agaataattc tccatcaggg aattcagcga aatgaatatc agaacaggga tgtgcgcttt
    17641 tttattgagc ttagtgctgc ctgcacaggc caccagcttt accgaatatc tgccaatgag
    17701 tgacagcgaa tacgcgcaaa agcgggcgct gaaacctctc ctgaccatgc cttatgatgc
    17761 cgagcaaacc tggcatttta gaaaagtggg cgttgctggc gtgacgcttg aaaagatgcc
    17821 aaatgatgat agtgaatggc aattgaacgg taaagatcgc gccggtaaat cctggtctgt
    17881 gcctgtgggt atgctggcaa acatagcggg caaagggcaa ctctatcgcg ccgatcttga
    17941 tcgtaacggc attcaggatc tggttatctg gttaccaagt accggtaatg gtctggctcc
    18001 ttatgcacat ttaattctta tgacgttcac gcgcgaaggc cgtccctgtg tctttgaacc
    18061 caggggattc tacaccgcaa gcaaaactgg cgtagatgat ttgctggatt tgcaaggcaa
    18121 tggtcacacg caattgctgg atatgcagtt tgattccggt tactggataa ccagtctgta
    18181 tcaggtaaaa gacgccaaat ggcagcgtgt tcacggctgg tttggcaagt taagctatcc
    18241 cgcgctcacg cgttttactt atacccccaa ccgtaaactc gtcctcaaac ccatcgccgg
    18301 gcgcgacccg caaacggaag atctggcgca gacgcagcgt tgcttgataa agggggatgt
    18361 tcttgatggg gtgaaccaga actgactttt tgcagagtgg ctatgaggat tttgggggta
    18421 attatatcgc gctcaagtgt ttacatattg tgaacttgga tttgatgagc tatagtttcc
    18481 gaacacataa ggatagaact cgcagatgtg gctgtttact tactataagg aagttaaaac
    18541 ttaaaaatgc acatcatttc taaagcgcca tttgaagagt ctgcgcgtaa atatcctaac
    18601 gacgctctgg ctcttcaggc attgtatcgg gttattaagg aaacggattt ttctacgcca
    18661 gaagagatgc gaacggcgtt tcccaacctc gacaatttta aatatcgcaa caaatggtac
    18721 gttctggatg taggtgggaa caacctaagg gtgattgcct acataaactt tgttaataag
    18781 cggttctttg tgaagcacat aactaatcac gccgagtatg acaaactgac acgttactac
    18841 agagagaaca aagaatgact gctaatgctg cacgcgctgt taaagcaaca agagaactgg
    18901 tcaatgcagt cccctttctg ggaggtagtg actcagaaga tgattatcgt gaagcactgg
    18961 aactggtcga atatttgatt gaagaagatg acaccaatcc acttatcgac tttctggcga
    19021 gccgcattgc agaatatgaa aacaacaacc aagagtttgc agagttcgac aaagccgttg
    19081 cggcaatgcc tgttggcgtg gcgttacttc gcacgctgat tgatcaacat aatctgacct
    19141 atgcggattt gaagaacgaa atcggctcta aatccctggt cagtcagatt ttgtctggac
    19201 aacgatccct gacgatatcc cacattaagg cactttcagc ccgatttggc gttaagccgg
    19261 aatggtttct ttgattttga ctgatgccaa accccagatt gcccgatgcg ctgcgcttat
    19321 cgggccttcc tggttcgtgc gacatgtagg ccggataagg cgttcacgcc gcatccggca
    19381 ctgttaccta ctctaaatct tccccattac tggcaatcac ttttttatac caccagaacg
    19441 atttcttacg tgtgcgcgtc agcgtgccgt tgcctgcgtc gtcacggtct acgtagacaa
    19501 aaccgtagcg tttgctcatt tcacccgtag aggcggagac taaatcaata cagccccagg
    19561 tggtgtagcc catcagcgga atgccatctg caatcgcttc gcccattgcg cggatatgtt
    19621 cgcgtaaata gctgatgcga tagtcatcgt taatctcgcc attggcagca agttcatctt
    19681 ttgcgccgag gccgttttcc accagaaaca gcggcttctg ataacggtcg tacatcatgt
    19741 tcatggtgat acgcagaccg agtggatcaa taccccagcc ccagtcgctc acttgcagat
    19801 acgggttacg cagcgatttc actacgttcg ccgcactgct attgttggcg ttcatctccg
    19861 ccgaggcgca gcgcgaggcg taatagctga aagagacaaa atcgacggtg tttttcagga
    19921 tttcatcatc gcccggtgct ttgtcgatgg ttaccccttt ttcgcggaat acgcgggcag
    19981 agtaagccgg atacgtgccc cgcgcctgca catcgataaa aaacaggttt tcccggtctt
    20041 tctccagcgc cgcccagaca tcttccggct tgcaactgta agggtagaag ttaccgcccg
    20101 ccagcataca gcccacctga ttttgcgggt taacctcatg ggcgattttg gtggctagcg
    20161 cactggcaac cagctggtga tgcgcggcct gatatttcac ctgatcctga ttttcacctt
    20221 cttcaaacac cagacccgcg ccggagaacg ggctatgcaa cataatgttg atttcattga
    20281 aggtaagcca gtatttcacc agaccatcaa atgcttcaaa gcaggtccgg gcgtagcggc
    20341 tgaaaaactc caccagcttg cggttacgcc aggagccata ttcggtgacc agatgcatcg
    20401 gcacatcgaa gtggcacaac gtgaccagcg gttcgatacc gtacttttta cactcttcaa
    20461 agacagaacg ataaaaagca atgccctgct gattgggcgt gatttcatcg ccctgcggaa
    20521 agagacggct ccaggcaatt gaggtacgga aaaccttgaa tcccatctct gccatcaggg
    20581 cgatatcttc tttataacga tgataaaaat ccgtcgcctc atggctggga taaaactcgt
    20641 catcgcgcaa ctgaaaacgt ttttccagcc ccagtttcac cgccattcga tgctcgccgt
    20701 gtgggatcat atcgacagtg gtcagccctt tgccaccttc acggaacgca ccttcagact
    20761 ggttggcggc aagcgcgccg ccccataaaa aaccttctgg aaatactgac attttcatcc
    20821 tcaattaaga cttacttctt tggcgacggt cggttgtacg ctctgatgct ttcgcgcctc
    20881 ggcagccgct tcctcaacag gaatatcctc aaagccgagc aacagtgtga ggataaacga
    20941 cagcaccacc gccagcgcca tgaccgcgaa cacccagacg atgctcattg gattcgccgg
    21001 atcgaagaat tgcacgctgg taaataaccc tggcgctgcc attgagtggc tggcaagccc
    21061 cgccataccg gcaaccgcgc cgcaaataaa accgctgata agactggcga taagcggacg
    21121 tttcaggcgg atcgccacgc cgtataacgc cggttcggaa atccccgcca taatggctga
    21181 tgccgccgca gccagcgccg tctggcgcag ttccgggttt ttcgttttcc acgccaccgc
    21241 cagtgatgaa ccgcccagcg acaggttagc gccgatctct gacggcatga ccatcccttc
    21301 tttgccggtt tcggcaatgg tctgaatgat ggttggcgta aagacgcggt gcatcccggt
    21361 cattaccagc agaggccaca gcgcgcccat aatggcgact gaaagccagc ccagataacc
    21421 atgaatggtg taaaccagcg ccgaaatggc gctaccgatc cagataccaa tcgggccaat
    21481 cagcaggatt gccagcggtg cggcaatcaa cacaatcagc attggcttga ggaagttttt
    21541 ggtcaccgcc ggtgtaatgc gatccaccca gcgttcgata tatgacaggc accaggtcat
    21601 caccagcgcc gggatcactg tgtaggtgta tttcaccgcg gtgaccggaa tcagggcaaa
    21661 ttcgacatgt tcaccctggg ccgctttcgc catcagttca ataaaactcg gatgtaccag
    21721 cacacccgcg atggcaatcg ccagcgacat attggtttta aatttgatgg cggcagatgc
    21781 cgcgaccatc agcggcagga agaagaaagc accgtcacca atcacattca gaatggttaa
    21841 ggtcggcgat ccttttgtca gcacgccgct catctcgagg atcatcgcca gcagtttgac
    21901 catcgatccg ccgataatcg ccgggatcag cggtgacatg gtgccgatca gcgcatcgag
    21961 gatccccgca ccaatgcgac gcagcgtgag tttgggttta cccacgggct gtgcgggctg
    22021 catatctccc ggcagcaggc tgacgacttc ctgaaaggct tgtgaaacgg tattgccgat
    22081 aatcacctga cactggttgt cactacgtac cacgccgagc acgccgggga tggtttttaa
    22141 cgtcgggctg tcgataagct ggtcatcttt gataacaacg cgcaaacgcg tcatacagtg
    22201 cgtgaccgcc gagatgttat caacgccgcc cagtgccgct atcaccgagc gtgccagcgc
    22261 cgcataattt ttggccatcg gattttatcc tgttatcagt agggtagatg tagttgttat
    22321 cagtcgacgt aacggaaaaa tgccgatgct gaaacggaat taggaaaccg gtttcacaaa
    22381 atcatgaata gttgtcacta aataagcaag agagttaatt gttcattttt tatatttgtg
    22441 aaaccggtca cctgaatatt tttcccgccg attgcgcaag gacaatcggc aaacggtgca
    22501 gtacactgcg ttgattgcgt gtaaagggat gaaaatgatg acgacgatgc tggaagtggc
    22561 gaagcgcgcc ggggtttcaa aagcgaccgt ttcccgcgtg ctttcaggta atggctacgt
    22621 cagccaggag actaaagatc gcgtgtttca ggcggtagaa gagagcggtt accgcccaaa
    22681 cttgctggcg cgcaatctgt cggcgaagag tactcagacg ctggggttgg tagtgaccaa
    22741 cacgctttac cacggcattt attttagtga attactcttt catgccgcgc gaatggcgga
    22801 agagaaaggg cggcagttgc tattggcaga tggtaaacac agcgccgaag aagagcgcca
    22861 ggcgattcag tatctgctgg atttgcgctg cgacgccatt atgatttacc cgcgtttttt
    22921 aagcgtggat gagatcgatg acatcattga cgcgcacagt cagccgataa tggtgcttaa
    22981 tcgccgcctg cgcaaaaaca gcagccatag cgtctggtgc gatcataaac agaccagctt
    23041 taatgccgtg gcagagttga taaacgccgg acatcaggag attgctttcc ttaccggctc
    23101 gatggattcc cccaccagca ttgaacgtct tgctgggtat aaagacgcgc tggcgcagca
    23161 tggtattgcg ctcaatgaaa aacttatcgc taacggtaaa tggacgcccg ccagcggggc
    23221 cgaaggggta gaaacgttgc tcgaacgtgg ggctaaattt agcgcgttag ttgccagtaa
    23281 cgacgatatg gcgataggtg cgatgaaagc gttgcacgag cgcggcgtag cggtgccaga
    23341 gcaggtgtca gttatcggat tcgatgatat cgcaattgcc ccctatatcg ttccggcgct
    23401 ctccagcgta aaaattccgg tgactgagat gattcaggaa attattggac ggctgatttt
    23461 tatgctcgat ggcggggatt tctcaccgcc gaaaaccttc agcggaaaac tgatccgccg
    23521 cgactcactc attgctcttt cgcgataaac attgtcatca catgtcatcg tcactagtgt
    23581 cgatgacatc ttttttcctg cattacagaa attaatttcc ttaaataaca gtgaattaaa
    23641 actggcatga tttgtgaatg tatcggcgca ttaactgtca ttgctggaga atttgatgaa
    23701 ccgtttcatc attgctgacg cgagtaaatg tattggttgc tgtacctgtg aagtggcgtg
    23761 cgtggtttct catcaggaaa atcaggactg tgcatcgctg accccggaaa catttttacc
    23821 gcgtatccat gtcattaaag gtgtgaacat ttccacggcg acagtttgcc gtcagtgtga
    23881 agatgcaccg tgcgctaacg tctgcccgaa tggtgctatc agccgtgata aagggtttgt
    23941 tcatgtgatg caggaacgtt gcattggttg caaaacctgc gttgtggctt gcccgtatgg
    24001 cgcgatggaa gtggtggtac gtccggtgat tcgcaacagc ggcgcggggc tgaatgtacg
    24061 ggctgacaaa gccgaagcca ataaatgtga cctgtgcaac catcgtgaag acggcccggc
    24121 gtgtatggcg gcttgcccga ctcatgcgct gatttgtgtc gatcgtaata aacttgaaca
    24181 actgagtgca gaaaaacgcc gccgtacggc gctgatgttc taaaaatctt acagaactgt
    24241 tctggcatca aaggaattcc cggaaatatt cgtagaatac ggagctggcg gtatcgccga
    24301 cctgagact
//
LOCUS       DAZOYL010000029        24269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZOYL010000029 DAZOYL010000000
VERSION     DAZOYL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.029"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(100..981)
                     /gene="prmA"
                     /locus_tag="ACHUOY_000865"
     CDS             complement(100..981)
                     /gene="prmA"
                     /locus_tag="ACHUOY_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR0681718.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(993..2444)
                     /gene="panF"
                     /locus_tag="ACHUOY_000866"
     CDS             complement(993..2444)
                     /gene="panF"
                     /locus_tag="ACHUOY_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR0681719.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2434..2676)
                     /locus_tag="ACHUOY_000867"
     CDS             complement(2434..2676)
                     /locus_tag="ACHUOY_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR0681720.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2785..4134)
                     /gene="accC"
                     /locus_tag="ACHUOY_000868"
     CDS             complement(2785..4134)
                     /gene="accC"
                     /locus_tag="ACHUOY_000868"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR0681721.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4145..4615)
                     /gene="accB"
                     /locus_tag="ACHUOY_000869"
     CDS             complement(4145..4615)
                     /gene="accB"
                     /locus_tag="ACHUOY_000869"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR0681722.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5593..6567)
                     /locus_tag="ACHUOY_000870"
     CDS             complement(5593..6567)
                     /locus_tag="ACHUOY_000870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR0681723.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6719..8659
                     /gene="csrD"
                     /locus_tag="ACHUOY_000871"
     CDS             6719..8659
                     /gene="csrD"
                     /locus_tag="ACHUOY_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR0681724.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAAQLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8964..10007
                     /gene="mreB"
                     /locus_tag="ACHUOY_000872"
     CDS             8964..10007
                     /gene="mreB"
                     /locus_tag="ACHUOY_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR0681725.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10073..11176
                     /gene="mreC"
                     /locus_tag="ACHUOY_000873"
     CDS             10073..11176
                     /gene="mreC"
                     /locus_tag="ACHUOY_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR0681726.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11176..11664
                     /gene="mreD"
                     /locus_tag="ACHUOY_000874"
     CDS             11176..11664
                     /gene="mreD"
                     /locus_tag="ACHUOY_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR0681727.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11673..12266
                     /gene="yhdE"
                     /locus_tag="ACHUOY_000875"
     CDS             11673..12266
                     /gene="yhdE"
                     /locus_tag="ACHUOY_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR0681728.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12256..13725
                     /gene="rng"
                     /locus_tag="ACHUOY_000876"
     CDS             12256..13725
                     /gene="rng"
                     /locus_tag="ACHUOY_000876"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR0681729.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13793..17593
                     /gene="yhdP"
                     /locus_tag="ACHUOY_000877"
     CDS             13793..17593
                     /gene="yhdP"
                     /locus_tag="ACHUOY_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR0681730.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17749..19194
                     /gene="tldD"
                     /locus_tag="ACHUOY_000878"
     CDS             17749..19194
                     /gene="tldD"
                     /locus_tag="ACHUOY_000878"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR0681731.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19322..20187)
                     /locus_tag="ACHUOY_000879"
                     /pseudo
     CDS             complement(19322..20187)
                     /locus_tag="ACHUOY_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            20370..20573
                     /gene="aaeX"
                     /locus_tag="ACHUOY_000880"
     CDS             20370..20573
                     /gene="aaeX"
                     /locus_tag="ACHUOY_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR0681732.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20581..21513
                     /gene="aaeA"
                     /locus_tag="ACHUOY_000881"
     CDS             20581..21513
                     /gene="aaeA"
                     /locus_tag="ACHUOY_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR0681733.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21519..23486
                     /gene="aaeB"
                     /locus_tag="ACHUOY_000882"
     CDS             21519..23486
                     /gene="aaeB"
                     /locus_tag="ACHUOY_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR0681734.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23578..23850
                     /locus_tag="ACHUOY_000883"
     CDS             23578..23850
                     /locus_tag="ACHUOY_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR0681735.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23906..24169)
                     /gene="yhcN"
                     /locus_tag="ACHUOY_000884"
     CDS             complement(23906..24169)
                     /gene="yhcN"
                     /locus_tag="ACHUOY_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR0681736.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5751 a         6136 c         6570 g         5812 t
ORIGIN      
        1 attttggcac gcaaatttgc gtaaataacc ggcaaattta cgtgcctttt gtgagcttgc
       61 tcgcacttcg ccccgcgtca ccctacggcg atgcgaaggt taattcttac gaccggtaat
      121 gcggcaccac tcttcttttt ccacgaccgg gtccagtgcg aagctgtcga cataagcttc
      181 acaaacgctc tctgcctggc ttgccagaat accggaaagg cccagcaaac cgcctgaaac
      241 cggcaggacg ctgattaacg gtgccagttc acgtaatggg cctgcaagga tgttagcgac
      301 caccacgtcg gctttcattt cttctggctg atctttcggt aagtagagtt ccagacggtc
      361 agaaacgcca ttacgttcgg cgttgtcgcg gctagcctga atcgcctgcg gatcgatatc
      421 aataccaatg gcttttgctg cacccagttt cagcgccgcg atagccagaa tgccggaacc
      481 acagccaaag tcgatgacta ttttaccggt taaatcgagg ctgtcgagcc actgcaggca
      541 cagagaggta gttggatggg tacccgtacc aaacgccagc cctggatcta acatcacgtt
      601 gacggcgttt tcgtccggca catcacgcca gctagggcag atccacagtc gttcaccaaa
      661 gcgcatcggg tggaaattat ccatccattc gcgctcccag tctttatctt ccagttgttc
      721 gattttatgc gcgaagcctg cgccgagcag cggatggttt tccagaatcg ccaccacgtc
      781 gttcatatcg gtttcagcgt cgaacagacc aatcacatcg gtgtcgcccc acaggcgcgt
      841 ttcgcccggc agcggctcaa atactggcgt atcgtgggta tcctgaaaag tgatagaaac
      901 ggcacccgct tccatcagcg catcgctaag atcttcagcg ttcgcgccgg tggtgttcag
      961 tttcagttgg atccaaggca tggcaaaact ctttatttat cagtagtcaa aacggtagct
     1021 tgcgggacgg atgtaccgaa acggtttccg accaggaaag ccagcaaact tagtagtaac
     1081 gagggcacga tagggtggaa gcccaggtac tgaatattca gcgtcgcgag tacggcatac
     1141 agcacgccgc caacgatcat cgcacttagc gcgcctttgg cgttggcgcg ttcccagtaa
     1201 agacccagca ccagcggcca caggaaaacg gcttccagcc caccgaaggc cagcaaattc
     1261 agccagatga tcatttctgg cggcttccag gcggcaagca gcagcaacgc gccgagaact
     1321 aacgtaatta ccgccgacat ccgcttcaga cgcgtctcgt tttgcatttg atccggacgg
     1381 atattcagat agagatcttt aatgatcgta gcggaacttt gtagcaattg ggcgttaatt
     1441 gtcgacatga tcgcagccat aggtgcagcc agaaagatcc cggcagcaaa cggtggcagc
     1501 acttttacca ttaacgtcgg gatcaccaga tccggtacgg tgagatcggg gatcactgca
     1561 cgacctaacg ctccggctag gtgcataccg aacatcagga ttgcgactac aatcgtaccg
     1621 atgatgatcc cccggtgtac cgctttgctg tctttataag agatacagcg caccgcagta
     1681 tgcggcaggc caatcacgcc aaagcacacc agtacccaga acgacgtcat aaaggcaggc
     1741 gacagaatat cgtcagcgcc ttgtggcgta accagttgcg gatcgatggt ttgcaaggtc
     1801 tgtactgcgt tgcttaagcc gccagcggca tgtaccacgc caataagcag cacaacggtg
     1861 ccaatcagca tcacaagccc ttgcatggtg tcgttcagca cgctggcgcg aaagccgcca
     1921 aaggcggtat acaacgcaat gctgataccg aaaatcagca gcccggtttc ataaggaata
     1981 cccgccgcgg tttccagcag gcgcgcaccg ccgataaact gcacggtcat tgccccaacg
     2041 aacgcaacca gcaaactcaa actcgccagc cacaccagaa gacgactctg gtagcgggca
     2101 aacagcatgt cgttcagcgt cactgcattg tagcggcgcg caagaatcgc aaacttcttg
     2161 ccgagaatac cgagtgaaag ccagactgca ggaagctgaa tcatcgccag caatacccag
     2221 cccagcccgt atttataagc agctcctggc ccgccgataa acgaactggc actgatatag
     2281 gtcgcggtga gcgtcatcgc cagcacaata ccgcccatag agcggctgcc gaggaaatac
     2341 tcattaagga aggtgccggt gctccgttta cgcatcgcat aaaccgagat accgaacacc
     2401 accaccagat aggcgaccag cggtagaatt acttcaagct gcatcgtcat cctccagtgg
     2461 gatatcgcga tagataaatt tcaccatcgc ccagcacagt ccaataaaca gtagcggcgt
     2521 caggatgcag gccatctcaa accagcgcgg aaagccggta aaaccggggg caacgccagg
     2581 taagtaagcg gctactaacc aaactgccag atacaaaagg gtcagcccca gcgcccagcg
     2641 cgcctcttta tgggcctgaa caaaacgagt gtccattttt tgtccctgat gggtgaagaa
     2701 agcgaggatt gtaccttatg ggggttgtcg atccccagta ataaaaaagg ccggaaaatc
     2761 cggccttttg gcgcttttgc agtcttattt ttcctgaaga ccgagttttt tctccagata
     2821 gtggatgtta gtgccaccat gctggaagtt ctcgtcattc atgatgcgga tctgcagatc
     2881 aacgttggtt ttgataccgt cgatgatcag ctcctgcagc gcattcttca tgcgggcaat
     2941 cgccacgtca cggttttcac cgtagcaaat cagcttaccg atcattgagt catagtacgg
     3001 cggtacggtg tagcccgcgt agatatgaga ctcccaacgt acgccaaaac cgccaggtgc
     3061 gtggaaacgg gtgattttgc ctgggcttgg caggaaggtg ttcggatctt cggcgttgat
     3121 acgacattcc accgcatggc cgcgaacgtg aacttcttct tgcttgatcg acagcggttg
     3181 accggcagcg atacggagct gttctttgat caggtcaacg ccagtgatca tttcggtaac
     3241 cgggtgttct acctgaatac gggtgttcat ttcgatgaaa tagaactcgc cgttttcgaa
     3301 cagaaactcg aaagtacctg caccgcgata gccgatatca acacacgctt tagcgcaacg
     3361 ttcgccgatg tagcgacgca gttccggggt aatgcccggt gctggcgctt cttcgaccac
     3421 tttctggtgg cggcgctgca tggagcagtc acgttccgcc agatagatag cgttgccccg
     3481 accgtcagcc agtacctgaa tctcgacgtg gcgaggattt tccaggtatt tctccatgta
     3541 aaccatatcg ttgctgaaag cagctttcgc ttccgcacgg gtcatggaga tggattgtgc
     3601 cagttcagcg tcgccgcgca ctacgcgcat accgcgacca ccgccgccgc cggaggcttt
     3661 gataatcacc ggataaccaa tgcgtttagc aatggcacgg tttttatcca tatcgtcgcc
     3721 caacgggccg tcagaacccg gtacgcaagg gacgcccgct tttttcatcg ccgcgattgc
     3781 ggatactttg tcgcccatca ggcgaatggt ttctgctttc gggccgatga agataaagcc
     3841 ggagcgttca acctgctcgg caaagttggc gttctcggag aggaagccgt aacccggatg
     3901 gattgctact gcgccggtga tttcagcggc gctgatgatt gccgggatgt tcagataact
     3961 ttttactgac ggagcagggc caatacagac cgtttcatct gccagtaata cgtgttttag
     4021 atcgcgatcc gcgctggagt gcacagcgac agtcttgatg cccagttctt tacaggcacg
     4081 aagaatacgc aatgcaatct cgccgcggtt ggcaataaca attttatcca gcatgttcgc
     4141 ctcgttactc gatgacgacc agcggctcgt caaattctac cggttgtcca ctttcgacca
     4201 gaattgcttt cacggtaccg gatttgtccg cttcgatctg gttcatcatt ttcatggctt
     4261 caacgatgca cagggtatcg cccacgttga ctttctgacc cacttcgatg aacgcttttg
     4321 cgtccgggct tggggtgcgg tagaaagtac caaccatcgg ggaacgtacg atgtgaccac
     4381 tgatttccgc tgctgctggc gcttccatgg aaggaacggt cgccggagcg gctgcgttag
     4441 attgagctgg ctgctgcatc attggtgcag cgtaagcttg ttgcatcaca gggaaacttg
     4501 cggcaggagc tgcacggcta atgcgtactg actcttcgcc ttcagaaatt tccagttcgg
     4561 agatgcctga ttcttcaacc agctcgatca gttttttaat cttacgaata tccatgagtg
     4621 ggttccgtac tctttgttta gtgtgattgt gacaggcgtt tcaccgccgt ctgtaaagcg
     4681 tatgccccga cagctgtatg catagcgata aattccagca ggccgggacg ccggtctatt
     4741 ttgccccaga gtatcaatgt tagacttgac ggacattgtg cagcgatctg ctgctatcct
     4801 ccggcaaaaa acaaaatata ccttcgttgc gccaatgtca ccttttccgc agcgcaaaaa
     4861 ctgcgtcagg gaggacgagg tcgcacatta taacgatttc gtagcaattg gcagctaaat
     4921 actggtctca tcagggaaga taatcaacag ctaacatgta aataaccttc aacaccgtgt
     4981 aatttgcaac aagccgcaca attcacgaaa ttagcgccat catcgacgga acttcttata
     5041 acgtaaggtg aaaagctgta aagccagccc tgcgaagatg aggggctgca atgagataat
     5101 cttcacagac cacaaataat gtagggatgc caggatcgcg acaagataga cgacattacg
     5161 cagcggttgc tggggattac ccaattttca ctgcatcgtc tggatagatg tgaatgctaa
     5221 agcacgcaaa atcaaccgac tgacaattct cagcattaaa taggttcgag tggttaattt
     5281 gtagtcaaat aaaatcagat tattcacccc atggtaccag aaatcagtaa caggttaagc
     5341 gtagtgtcgc ccaggcaaag caccataaac ctaacaggcg acggaggcgt atcaataatg
     5401 gctgtttaga ggagtatcaa tgcgcatcaa tttgtggtca gtcgaaatca tgactttcca
     5461 gtttggatga tgttgtacaa cttcctgacc aaggtgttcg acgaaagctg tccggctaac
     5521 cgcttttccc gttaaccgat tgaataatga tgtcaaaatg agttccctct cttattattc
     5581 cttgctaaat ggttagttaa ccttcaccag cgtgcgaccc tggatctggt tattaatgat
     5641 ggcctcggca aagttcggtg cctctgacag agatatctct tttgccgcct gggtatagaa
     5701 tgattccggt aaatcggcga ccagtcgctg ccaggcttgt gcgcggcgtt ctggtggcgt
     5761 cattactgaa tccacccctt gcaaacggac attacgcaga ataaatggca tgaccgtggt
     5821 tggcagagta aaaccacccg ccagaccaca ggccgccacg cagccgccgt aattcatttg
     5881 cgccagcact tttgccagca ctttgtcgcc aacagtgtca attgccccag cccagacttg
     5941 tttttccaga ggacgggatt cggcaaactc atcacgaggg agaatacggc tggcacctaa
     6001 acttttcaga tattcatggg tactttcgcg accggaaacg gcaacgacct gataacccaa
     6061 cttatgcagc agcgccacgg cggtactgcc gacgccacca ctggcacccg tcacgacaat
     6121 ctccccgtcc tgcgggcgaa caccggcatc ttccagcgcc atcacacaca gcatggcggt
     6181 aaaaccggca gtaccgataa tcattgcttt acgcgcgtcc agcccttgcg gcatggcaac
     6241 cagccagtca cctttcactc gcgcctgctc cgccagccca ccccagtggt tttcaccaac
     6301 gccccagcca gtgagtaaca cctcctgacc ggcatgaaaa cgcggatctt cgctggtgcg
     6361 tacagttccg gcaaaatcga tcccaggaat catcggaaaa ttacggatga tttttccctt
     6421 accggtaatc gccagcgcat ctttatagtt caggctcgac cagtgaacat cgaccgtgac
     6481 atcgccctcc ggcaggcgac tttcgtccag agtctgtact gatgcgagag ttttgccgtc
     6541 ctgctgttct aaaagtaacg cctgcataag tggtcctcat gtgcatgatg gattggaaaa
     6601 taatttctga agattatact cgctaatgga aaagcaaagc cgatgaagcg caataaattg
     6661 cgagataaat ctgatttgct agtatgcccg cttcctcact atcggagtta acacaaggat
     6721 gagattaacg acgaaatttt cggcctttgt tacgctgctc accgggttaa caatttttgt
     6781 gactttgctg ggctgttcgc taagtttcta caacgcaatt cagtataagt ttagtcatcg
     6841 cgttcaggcg gtggcgacgg cgattgatac ccaccttgtg tcgaatgact tcagcacatt
     6901 aaggccacaa attaccgaat taatgatgtc ggcagatatc gttcgtgtag acctgctcca
     6961 tggtgataaa caggtttata ccctggccag aaatggtagt tatcgtccgg ttggctccag
     7021 cgatctgttt cgcgaactga gtgttccgtt gataaagcat ccggggatgt cgctgcgtct
     7081 ggtttatcag gatccgatgg gcaactattt ccattcttta atgactaccg cgccgctcac
     7141 gggggcgatt ggctttatca ttgttatgct cttcctggcg gtacgctggt tacaacggca
     7201 acttgccggg caagaattgc tggaaacccg ggctactcgt atcttaaacg gtgagcgtgg
     7261 ctctaatgtg ttgggaacca tctatgaatg gccgcccaga accagcagtg cgctagatac
     7321 gctgcttcgt gaaattcaga acgcacgcga acaacacagc cgtcttgata cgctgatccg
     7381 ctcttatgcc gcccaggacg tgaaaaccgg cctcaataac cgactctttt tcgataatca
     7441 gttagcaacg ttactggaag atcaggagaa agtaggtacc cacgggatcg tgatgatgat
     7501 tcgtctgccg gatttcaata tgttgagcga cacttggggg cacagccagg ttgaagaaca
     7561 gttcttcact ctgactaatc tgctgtcgac atttatgatg cgctaccctg gcgcactgct
     7621 ggcgcgttac caccgcagtg attttgctgc gcagttaccg caccggacgt taaaagaggc
     7681 agagagcatc gccggtcagt taatcaaagc cgtcgatacc ttgccgaaca ataaaatgct
     7741 cgatcgcgac gatatgatcc acattggtat ctgcgcctgg cgtagtggtc aggataccga
     7801 gcaggtaatg gaacatgcag agtctgccac gcgtaatgcg ggattgcagg gcggcaatag
     7861 ctgggctatt tacgatgact cgttgcctga aaaaggacgc ggtaatgttc gctggcgtac
     7921 gcttatcgag caaatgctca gtcgcggcgg cccgcgcctt tatcaaaaac cggcggttac
     7981 tcgcgaaggt caggttcatc atcgcgaact catgtgccgc atcttcgatg gtaatgaaga
     8041 ggttagctcg gcggagtata tgccgatggt cttgcagttt ggcttatcgg aagagtatga
     8101 ccgtctgcaa atcagccgtc ttattccact attgcgttac tggccagagg aaaatctggc
     8161 gattcaggtt accgttgagt cgctgattcg cccgcgtttt cagcgttggc tgcgcgatac
     8221 gttaatgcaa tgtgaaaaat cacaacgaaa acgcataatt attgaacttg cagaggccga
     8281 tgtaggtcaa catatcagtc gtttacaacc tgttattcgt ttagtgaatg ctttaggggt
     8341 acgggtagcc gtcaaccagg ctggtttgac gctggtaagc accagttgga tcaaagaact
     8401 taatgttgag ttactcaagc tccatccggg gctggtcaga aacattgaga agcgaacgga
     8461 gaaccagctg ctggttcaaa gcctggtgga agcctgctcc gggaccagca cccacgttta
     8521 cgccaccggc gtgcgttcgc gaagcgagtg gcagaccctg attcagcgcg gtgttacagg
     8581 cgggcaaggg gattttttcg cgtcctcaca gccacttgat actaacgtga aaaaatattc
     8641 acaaagatac tcggtttaac ctgccgttta atccgttttc acgtagaata acgcgcgctg
     8701 cgtctcatgg gagtgtgctt gtctgctcgc cagattgttg cagcacatat gcagatgaat
     8761 gaccttacgc ggttgcaaac aggcgaggaa tgctgctgat gcattaagcc tttctggact
     8821 caggcagaga tttgtaacaa aggaaacgaa ctgcactaat tttcaccgta gcagatgatt
     8881 tttgcgcctt gtcgctgctg cgtgtggttg gtaaagtaag cggattttct tttccgcccc
     8941 agctttcagg attatccctt agtatgttga aaaaatttcg tggcatgttt tccaatgact
     9001 tgtccattga cctgggtact gcgaataccc tcatttatgt aaaaggacaa ggcatcgtat
     9061 tgaatgagcc ttccgtggtg gccattcgtc aggatcgtgc cggttcaccg aaaagcgtag
     9121 ctgcagtagg tcatgacgcg aagcagatgc tgggccgtac gccgggcaat attgctgcca
     9181 ttcgcccaat gaaagacggc gttatcgccg acttcttcgt gactgaaaaa atgctccagc
     9241 acttcatcaa acaagtgcac agcaacagct ttatgcgtcc aagcccgcgc gttctggttt
     9301 gtgtaccggt tggcgcgacc caggttgaac gccgcgcaat tcgtgaatcc gcgcagggcg
     9361 ctggtgcccg tgaagtcttc ctgattgaag aaccgatggc tgccgcaatt ggtgctggcc
     9421 tgccggtttc tgaagcgacc ggttctatgg tggttgatat cggtggtggt accactgaag
     9481 ttgctgttat ctccttgaac ggcgtggttt actcctcttc cgtgcgcatt ggtggtgatc
     9541 gtttcgacga agctatcatc aactatgtgc gtcgtaatta cggttctctg atcggtgaag
     9601 ccaccgcaga acgtatcaag cacgaaatcg gttcggctta cccgggcgat gaagtccgtg
     9661 aaatcgaagt tcgtggccgt aacctggcag aaggtgttcc acgcggtttt accctgaact
     9721 ccaacgaaat cctcgaagca ctgcaggaac cgctgaccgg tattgtgagc gcggtaatgg
     9781 ttgcactgga acagtgcccg ccggaactgg cttccgacat ctccgagcgc ggcatggtgc
     9841 tcaccggtgg tggcgcactg ctgcgtaacc ttgaccgttt gttaatggaa gaaaccggca
     9901 ttccagtagt tgttgctgaa gacccgctga cctgtgtggc gcgcggtggc ggcaaagcgc
     9961 tggaaatgat cgacatgcac ggcggcgacc tgttcagcga agagtaatcg gatgcaggca
    10021 ggggaaatgt ctgtttaccc tgcctggtct gatacgagaa tacgcataac ttatgaagcc
    10081 aatttttagc cgtggcccgt cgctacagat tcgccttatt ctggcggtgc tggtggcgct
    10141 cggcattatt attgccgaca gccgcctggg gacgttcagt caaatccgta cttatatgga
    10201 taccgccgtc agtcctttct actttgtttc caatgctcct cgtgaattgc tggatggcgt
    10261 atcgcagacg ctggcctcgc gtgaccaact agaacttgaa aaccgggcgt tacgtcagga
    10321 actgttgctg aaaaacagtg aactgctgat gcttggacaa tacaaacagg agaacgcgcg
    10381 tctgcgcgag ctgctgggtt ccccgctgcg tcaggatgag cagaaaatgg tgactcaggt
    10441 tatctccacg gttaacgatc cttatagcga tcaagttgtt atcgataaag gtagcgttaa
    10501 tggcgtttat gaaggccagc cggtcatcag cgacaaaggt gttgttggtc aggtggtggc
    10561 cgtcgctaaa ctgaccagtc gcgtgctgct gatttgtgat gcgacccacg cgctgccaat
    10621 ccaggtgctg cgcaacgata tccgcgtaat tgcagccggt aacggttgta cggatgattt
    10681 gcagcttgag catctgccag cgaatacgga tattcgtgtt ggtgatgtgc tggtgacctc
    10741 cggtctgggc ggtcgtttcc cggaaggcta tccggtcgcg gttgtctctt ccgtaaaact
    10801 cgatacccag cgcgcttata ctgtgattca ggcgcgtccg actgcaggac tgcaacgttt
    10861 gcgttatctg ctgctgctgt ggggcgcgga tcgtaacggc gctaacccga tgacgccgga
    10921 agaggtgcat cgtgttgcta atgaacgtct gatgcagatg atgccgcagg tattgccttc
    10981 gccagacgcg atggggccaa agttacctga accggcaacg gggatcgctc agccgactcc
    11041 gcagcaaccg acgacaggaa atgcagctac tgcgcctgct gcgcccacac agcctgctgc
    11101 taatcgctct ccacaaaggg ctacgccgcc gcaaagtggt gctcaaccgc ctgcgcgtgc
    11161 gccgggaggg caatagtggc gagctatcgt agccagggac gttgggtaat ctggctctct
    11221 ttcctcattg cgctgttgct gcaaatcatg ccctggccgg ataacctgat tgttttccgg
    11281 ccaaactggg tgttactcat cttgttgtat tggatcctgg ccttgcctca tcgcgtaaat
    11341 gtgggcacag gttttgtgat gggtgccata ctggatctga tcagcggctc gacgcttggc
    11401 gtacgcgtat tggcgatgag catcattgct tacctggtgg cgctgaaata ccagcttttc
    11461 cgcaacctcg cattatggca gcaggcgctg gtcgtcatgt tgctttcgct ggtggtggat
    11521 attattgttt tctgggcaga gtttttagtg attaacgtct ctttcagacc ggaagtgttc
    11581 tggagtagtg tagtcaatgg ggtgctctgg ccgtggattt tcttgctgat gcgtaaagtc
    11641 cgtcagcagt ttgcagtgca ataaaggttt ctatgacttc tctgtattta gcttccggtt
    11701 ctccgcgtcg tcaggagtta cttgcgcaac ttggtgtgac ctttgaacgt attgttacgg
    11761 gcattgagga gcagcgtcag ccgcaggaga gcgcgcagca gtatgttgtg cgtctggcgc
    11821 gcgagaaagc acaggcgggt gtcgcgcaaa cggcgcagga tctcccggtg ctgggtgcgg
    11881 atactatcgt tatcctgaat ggagaagtgc tggagaaacc gcgcgacgca gagcatgcgg
    11941 cgcagatgtt gcgcaaatta tcgggtcaga cccatcaggt gatgacggca gtggcgttgg
    12001 ccgacagcca gcacattctc gattgcctgg tggtcaccga tgtgactttc agaacgttaa
    12061 cagacgaaga catcgcgggc tatgtcgcca gcggtgaacc gttagataaa gcaggtgcat
    12121 acggtattca ggggctgggt ggctgttttg tcaggaagat aaatggcagc tatcacgccg
    12181 tagtcggctt acctctggtt gaaacgtatg aattattaag taattttaac gcactgcgtg
    12241 agaaaaggga taaacatgac ggctgaattg ttagtaaacg taacgccttc ggaaacgcga
    12301 gtggcgtata ttgatggcgg tattctgcag gaaattcata ttgaacgtga ggcgcgacgc
    12361 ggaatagtag gcaatatcta caagggtcgt gtaagtcgtg tacttccggg tatgcaggcg
    12421 gcttttgtag atattgggct ggataaagcc gcgtttcttc atgcatccga tatcatgccg
    12481 cacaccgaat gtgtggcggg tgaagaacaa aagcaattca cggtgcgcga catctcggaa
    12541 ctggttcgtc aggggcaaga tctgatggtg caggtggtga aagatccgct tggcactaaa
    12601 ggtgcgcgcc tgaccaccga tatcacgctg ccttctcgct atctggtctt tatgccaggg
    12661 gcttctcacg ttggggtttc ccaacgtatt gaaagcgaat cagaacgtga acgcctgaaa
    12721 aaagtggtcg cagagtattg cgacgagcag ggcgggttta tcatccgtac cgcagcggaa
    12781 ggggttggcg aggctgaact ggcctccgat gccgcttatc tgaaacgcgt ctggaccaaa
    12841 gttatggagc gtaaaaagcg cccgcagacc cgttatcagc tgtacggcga actggcgctg
    12901 gctcagcgtg ttctgcgtga tttcgccgac gccgaactgg accgcattcg cgttgactca
    12961 cgcctgactt acgaagcatt acttgagttc acctcggagt acattcccga gatgaccagc
    13021 aagctggagc attacaccgg acgccagccg attttcgatc tctttgatgt cgaaaacgaa
    13081 atccagcgag cgctggaacg caaagtagaa ctgaaatccg gtggttatct cattatcgac
    13141 cagaccgaag cgatgaccac cgtggacatc aataccggag cgtttgtcgg tcatcgcaat
    13201 ctggacgaca ccattttcaa taccaatatt gaagcgacgc aggctatcgc tcgccagtta
    13261 cggttgcgta atctgggcgg gattatcatt attgatttca tcgatatgaa taatgaagat
    13321 caccgccgcc gcgtgctgca ctcgctggag caggcgttga gcaaagaccg ggtgaaaacc
    13381 agcgttaatg gtttttcggc gctggggctg gtggagatga cgcgtaaacg cacccgcgaa
    13441 agcattgagc acgtactgtg taacgaatgc ccaacctgcc acggtcgcgg aacggtgaaa
    13501 accgtggaaa cggtatgcta tgaaatcatg cgcgagattg ttcgtgtcca ccatgcttac
    13561 gactccgacc gtttcctggt ctatgcttct ccggcagtag ccgaagcctt gaaaggcgaa
    13621 gagtcacact cgctggcgga agtggaaatt ttcgttggca aacaggttaa agtacaaatt
    13681 gaaccgctct ataaccagga gcagtttgac gtcgtaatga tgtaaacaga tgctgggccg
    13741 ccatccggca aagggttttt gagtcacatt tttagcagac aaggagtgac gggtgaggcg
    13801 attgccgggg attttactgc ttactggagc cgcgctcgtt gtgatcgctg ccctgctggt
    13861 tagcggcctg cgtattgctt taccgcatct tgacgcctgg cgtccggaaa tcctcaacaa
    13921 aatagaatcc gcgactggca tgccggtaga agccagtcag ctctcagcca gctggcagaa
    13981 ttttggcccg acgcttgaag cacacgacat ccgtgcagaa ctaaaagatg gcggcgaatt
    14041 ttcggttaaa cgcgttactc tggcgctgga tgtctggcag agcctgttac atatgcgctg
    14101 gcagtttcgc gacctcactt tctggcagct gcgctttcgc accaacactc ctatcaccag
    14161 cggtggtagc gatgacagtc tggaagccag tcacatcagc gatctgtttc ttcgtcaatt
    14221 tgaccatttc gatctccgcg acagtgaagt cagtttcctg acgccatccg gtcagcgtgc
    14281 cgagctggcg atcccacaac tcacctggct gaacgatcca cgtcgacacc gtgcggaagg
    14341 cctggtaagc ctctccagcc ttaccggaca gcacggcgtg atgcaggtgc gcatggattt
    14401 gcgcgatgat gaggggttgt taagcaatgg tcgcgtctgg ctacaggcgg atgacatcga
    14461 cctgaagccg tggctcggta aatggatgca ggacaatatt gctctggaaa cggcacagtt
    14521 ctcccttgaa ggctggatga cgatcgacaa aggcgatgta accggcggtg acgtctggct
    14581 gaaacagggc ggtgccagct ggttgggcga gaagcaaacg catacgctgt cggtggataa
    14641 tctgaccgcg catattacgc gtgaaaatcc gggctggcag ttctctattc ccgatacacg
    14701 gatcacgatg gacggcaaac cctggccgag cggagcattg acgctggcct ggataccgga
    14761 acaggacgtt ggcggcaaag acaataaacg cagtgacgaa ctccggattc gcgccagtaa
    14821 tctggagctg gcaggcctgg agggcatacg cccgctggcc gcgaaacttt cacctgcact
    14881 gggtgatgtt tggcgctcca cacaaccgag cggcaagatt aacactctgg cgctggatat
    14941 cccgcttcag gcggcagaca agacccgttt tcaggcatcg tggagcgatc tggcctggaa
    15001 gcaatggaaa ttattaccgg gtgcggaaca cttctccggg acgctttccg gcagcgttga
    15061 aaatggtttg cttaccgcgt cgatgaagca ggcaaagatg ccttacgaaa cggtattccg
    15121 tgcgccactg gaaatcgccg acggccaggc aactataagc tggctgaaca atgacaaagg
    15181 tttccagctg gatgggcgta atattgacgt taaagccaaa gccgtccatg cgcgcggcgg
    15241 ttttcgttac ctgcaacctg ctaacgatga accctggctg ggtattctgg ctggcatcag
    15301 taccgatgat ggttcacaag cctggcacta tttcccggaa aacttgatgg gtaaagacct
    15361 ggttgattac ttaagtggcg cgattcaggg cggtgaagcg gataacgcga cgctggttta
    15421 tggtggcaat ccgcaactct tcccctataa acacaacgaa ggtcagtttg aagtgctggt
    15481 gccgctgcgc aacgcgaagt ttgccttcca gccggactgg cctgcattaa ctaaccttga
    15541 tattgaactg gactttatta acgacggttt atggatgaaa accgatggcg ttaatctggg
    15601 cggcgtgcgc gcgagtaatc tcaccgcagt gatccctgac tactcgaaag aaaaactgct
    15661 gattgacgct gacattaaag gtccgggtaa agccgttggc ccttactttg atgagacacc
    15721 gctgaaagat tctctgggtg cgaccctgca agaactccag ctcgacggcg atgtgaatgc
    15781 tcgcttacat cttgatatcc cgctgaacgg cgagctggta accgcgaaag gtgaagtgac
    15841 gctgcgtaat aacagtctgt ttatcaaacc actcgacagc accctgaaaa atttgagcgg
    15901 taaattcagc tttatcaatg gcgatctgca aagtgaacca ctgacagcaa gctggtttaa
    15961 tcagccgttg aacgtggatt tttccaccaa agaaggggca aaagcctacc aggttgcggt
    16021 gaatctcaac ggtaactggc aaccggcgaa aaccggcgtt ctgcctgaag cggtgaacga
    16081 agcattgagt ggcagcgtgg cgtgggatgg taaagtgggc attgatctgc cttatcatgc
    16141 tggcgcgacc tataacgtag agctgaacgg cgatctgaat aatgtgagta gtcacttacc
    16201 ttcaccgtta gccaaacctg cgggtgaacc actagcggta aacgttaagg ttgatggcaa
    16261 tctcaacagc tttgaattaa ccggacaggc tggtgcggat aaccatttca atagccgctg
    16321 gttgcttggt caaaagctga cgctcgatcg tgctatttgg gcggcagaca gtaaaacgct
    16381 cccgccgttg ccggaacaaa gtggcgttga actcaatatg ccgccgatga atggtgccga
    16441 gtggctggcc ctgttccaga aaggcgctgc ggagagtgtc ggtggtgcag cgagtttccc
    16501 acaatacata acgttacgta cgcctatgtt gtcactggga aatcagcaat ggaataacct
    16561 gagtattgtt tcgcaaccga cggcaaatgg caccctggtt gaggcgcaag ggcgtgaaat
    16621 caacgccacg ctagcgatgc gtaataacgc gccgtggctg gcgaatatca aatatcttta
    16681 ttacaacccg agcgtggcga aaactcgtgg tgattcaacg ccgtcatcac ctttcccgac
    16741 aacggagcgc attaacttcc gtggctggcc ggacgcacaa atacgatgca cagagtgctg
    16801 gttctggggg caaaaattcg gtcgcattga cagtgatatc accatttctg gcgatacgtt
    16861 aacgctgacc aatggactga ttgatactgg tttctcgcgg cttactgccg atggtgaatg
    16921 ggttaataat ccggggaatg aacgtacctc gctgaaagga aaactgcgcg ggcagaaaat
    16981 tgatgccgcc gcagaatttt ttggtgtcac gacgcccata cgccagtcgt catttaatgt
    17041 ggattacgat ttacactggc gcaaagcacc ctggcagcca gatgaagcga cgttgaatgg
    17101 catcattcat actcaactgg gtaaaggcga aattaccgaa atcaataccg gacatgccgg
    17161 gcaattgctg cgcttattga gcgtagatgc cctgatgcgt aagctgcgtt ttgatttcag
    17221 agacactttt ggcgaagggt tctattttga ctccattcgc agcaccgcgt ggattaaaga
    17281 cggggttatg cacaccgacg acacgctggt ggatggcctg gaggcggata tcgccatgaa
    17341 agggtcggta aatctggtac gtcgcgacct gaatatggaa gcggttgtcg caccagagat
    17401 ttctgcgacg gtgggcgtgg ctgcggcttt tgcggttaac cccattgttg gcgcggcagt
    17461 ttttgccgct agtaaagtgc tggggccgct gtggagcaaa gtctccattt tgcgctatca
    17521 catttcgggt ccgctggacg atccgcaaat caacgaagtg ttgcgccaac cgcgtaaaga
    17581 aaaagcgcaa tgatttgacg agggcgcgta attgccccaa tctcatatga taatcgttgc
    17641 caaaggccaa cgagccagaa cataaccgta ggccggatag ggcgttcatg ccgcatccgg
    17701 cagccgtaaa aaatcctcta ctgcagtaac taacgagtag caaaaacgat gagtctgaac
    17761 ctggtaagtg aacaattgct agcggcgaac agcctgaaac atcaggactt gttcgcgatc
    17821 ctcggtcaac tggccgaacg tcgccttgat tatggcgatc tctattttca gtcgagctat
    17881 cacgaatcct gggttttaga agaccgcatt attaaagatg gttcttacaa catcgatcag
    17941 ggcgttggcg tgcgtgcaat cagcggtgaa aaaaccggat ttgcttacgc tgaccaaatc
    18001 agcctgctgg cgctggaaca gagtgcgcaa gcggcgcgca ccatcgtccg tgatagtggt
    18061 gatggcaaag tacagacgct gggcgcggta gagcatagcc cgttgtatac ctcggtagat
    18121 ccgctgcaaa gcatgagccg tgaagagaag ctggatatcc tgcgtcgcgt cgataaggtt
    18181 gctcgcgaag cggacaagcg cgtacaggaa gtgactgcca gcctcagtgg tgtctatgaa
    18241 ttaattctgg ttgcggccac cgacggcacg ctagcggcgg atgtccgtcc gctggtgcgt
    18301 ctttccgtga gcgttctggt cgaagaagat ggcaaacgcg aacgcggtgc cagtggcggc
    18361 ggcggtcgtt ttggttatga gttcttcctt gccgatcttg acggcgaagt ccgtgcggat
    18421 gcatgggcaa aagaagcagt gcgtatggcg ctggtcaatc tttctgccgt tgctgcacca
    18481 gcgggcacca tgccggtagt acttggcgca ggttggccgg gcgtgctgtt gcatgaagcg
    18541 gtaggtcacg gtctggaagg cgacttcaac cgccgcggca cttcagtatt tagtgggcag
    18601 gtcggggagc tggtggcttc agaactgtgt accgtggttg atgacggcac gatggtcgat
    18661 cgccgtggtt cggtggcgat tgatgacgaa ggtacgccag gccagtacaa cgtgctgatt
    18721 gagaacggca ttctgaaagg ctacatgcag gataaactca acgcgcgttt gatggggatg
    18781 acgccgactg gcaacggtcg ccgtgaatcc tacgcccatc tgcccatgcc gcgtatgact
    18841 aacacctata tgctgccggg taaatcgacc ccgcaggaaa ttattgaatc cgttgagtac
    18901 ggtatctatg caccgaactt tggtggcggt caggtggata tcacctccgg caaattcgtt
    18961 ttctccactt cagaagcata tctgattgaa aacggtaaag taacgaagcc ggtgaaaggc
    19021 gcaacgttga ttggttccgg tatcgaaacc atgcagcaga tttcgatggt tggcaacgac
    19081 ctgaaactgg ataacggcgt gggtgtctgc ggtaaagaag ggcaaagttt gccggttggc
    19141 gtgggccagc caacgctgaa agttgataac ctgactgttg gcggtactgc gtaataattt
    19201 cattattttc agaaggataa ttaatctttc tacgtgcacg aacggtcccc tcgctcctct
    19261 ggggttaggg tgaggggaac ccgttggcac aggtttgtag aacgtaacag tacaatatga
    19321 attacttctc tttcccgcgc ccgtgcatct cctgaaacaa tttaccgacc tcaacaaaat
    19381 aatccgtcag cgagttgatc acgacctgta ccttcagcgg cagcttatct ttttcggtat
    19441 ataacgcata aaccgggcgt ggatctgact gataacgcgg cagcaggatc tccagctcac
    19501 cgcgattgat ctcgttgatc acccacatca gcggcacgta ggcgatcccg gcacccgccg
    19561 tcagccagcg caccagcgtc atcggatcat tagtcacaaa tcttccttgt gggatcaggc
    19621 gagtcgagat cccttccggt gcgatcagtt caaattcatt gtcgggccgc acgctgtatt
    19681 caagccatga atgactactc aaatcggcgg gtttttccgg tatgccgtat tgtgtgagat
    19741 agcttttcgc ggcgcacacc accatcggca tcgcgcccag acggcgggaa aacaggctgg
    19801 aattccggta accagattga cgctcaaacc tgggtattct ttcagcattt ttgctgtcag
    19861 cccggcgaga acattttgtg ccatagttga agaacagcca atgcgcagcg tcccgatggg
    19921 ggtgttattg aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatccgacg
    19981 gcagccctgg tagtaaattc tgccggcttc ggtcaggcca atgctgcgtg tgctacggtt
    20041 taacagcttt acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac
    20101 gctcatctgt agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa
    20161 caccgacatg cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac
    20221 ataatataga taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg
    20281 ttgctctcgc ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg
    20341 cctgctctct ctttacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg
    20401 gactgtcctt cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg
    20461 tgcgccgggt acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca
    20521 acaccgcgct ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa
    20581 gtgaaaacac taataagaaa attctcccgt acggccatca cggtcgtatt agtcattcta
    20641 gccttcatcg caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac
    20701 gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag
    20761 gtgaatgttc atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag
    20821 ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg
    20881 gcacaggaga aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct
    20941 cgcgaagaga tcgaccaggc caacaacgta ctacaaacgg ttctgcatca gttagcgaaa
    21001 gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgcgcgcca
    21061 gcagatggct gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca
    21121 acggcggttg cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact
    21181 aagctggaag gggggcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa
    21241 gtgctgaaag ggactgttga tagtgttgcc gcaggggtca ccaacgccag cagcacgcgt
    21301 gacgacaaag ggatggcgac tatagactct aatcttgaat gggtgcgtct tgcgcaacgt
    21361 gttccggttc gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact
    21421 gctacagtgg tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt
    21481 aaaatggccc atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta
    21541 accaacatat tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg
    21601 gctttcactt ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg
    21661 ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct
    21721 ttttgcgcat catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga
    21781 tgatccgcgc accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct
    21841 ggatatcctc gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg
    21901 cgctgatcat tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg
    21961 aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt
    22021 ctccgcgatc gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat
    22081 atcaattaat gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg
    22141 gcgacctggt gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat
    22201 cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc
    22261 tgattaccca atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg
    22321 atactttccg cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc
    22381 tcaaacgcct gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt
    22441 atagctgggt cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca
    22501 caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt
    22561 cagccgaacg tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg
    22621 gcacgctttt ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg
    22681 cggtcgtgac gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta
    22741 tctatgggac gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta
    22801 atacccaaca gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg
    22861 gtatagaagt acagaaacgg cgactgggct cgatgggggc actggccagc accataaata
    22921 ttatcgtgct ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag
    22981 ggcaaatcgt cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc
    23041 gcgacaggac tggacgtgta ctgcttaatc agtttgtttt tgccgctgtt tccgcgatga
    23101 ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt
    23161 tgctgatgaa taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta
    23221 tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc
    23281 accgacaaat gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc
    23341 gtcggtactt tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct
    23401 ggcaagcgcc accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt
    23461 atcaacatgc gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca
    23521 tggctatact tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg
    23581 aatatttata cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt
    23641 agccaaacct ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg
    23701 ttaatgaacg atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa
    23761 acgcgtcgcc gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa
    23821 gggcatttgc gttttaatgt tcgtcattag cgcaaaaaaa agcccccgaa ccgggggcaa
    23881 tatcgtcgga caagacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt
    23941 caccgctacg agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt
    24001 tcagcatttc acgcatatcc attggcgaag acgccacacc acttacggat acggtcccga
    24061 ttgcttcacg attttgtgct tgtgcagtat caatggagtc ggcagcgaat gcaccgaaag
    24121 agagaacaga aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct
    24181 cgacataatc ttttagctgg gcctttgttt cgtgatccat atcacaaaat caagcataca
    24241 ctaattattg aactaattaa taccacact
//
LOCUS       DAZOYL010000030        23483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZOYL010000030 DAZOYL010000000
VERSION     DAZOYL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.030"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUOY_000885"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUOY_000885"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR0681737.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHUOY_000886"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHUOY_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR0681738.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHUOY_000887"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHUOY_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR0681739.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHUOY_000888"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHUOY_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR0681740.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHUOY_000889"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHUOY_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR0681741.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHUOY_000890"
     CDS             5280..5738
                     /locus_tag="ACHUOY_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR0681742.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHUOY_000891"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHUOY_000891"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR0681743.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHUOY_000892"
     tRNA            6790..6874
                     /locus_tag="ACHUOY_000892"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="ACHUOY_000893"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="ACHUOY_000893"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHUOY_000894"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHUOY_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0681744.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHUOY_000895"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHUOY_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0681745.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHUOY_000896"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHUOY_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0681746.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCNPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="ACHUOY_000897"
     CDS             complement(10156..13899)
                     /locus_tag="ACHUOY_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR0681747.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHUOY_000898"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHUOY_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR0681748.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="ACHUOY_000899"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="ACHUOY_000899"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR0681749.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="ACHUOY_000900"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="ACHUOY_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR0681750.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHUOY_000901"
                     /gene_synonym="iatA"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHUOY_000901"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR0681751.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="ACHUOY_000902"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="ACHUOY_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR0681752.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHUOY_000903"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHUOY_000903"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR0681753.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHUOY_000904"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHUOY_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR0681754.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="ACHUOY_000905"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="ACHUOY_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR0681755.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="ACHUOY_000906"
     CDS             23294..23401
                     /locus_tag="ACHUOY_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0681756.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT         5883 a         6142 c         5664 g         5794 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cgaatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcgacc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggattgc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc aatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttg
//
LOCUS       DAZOYL010000031        23459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZOYL010000031 DAZOYL010000000
VERSION     DAZOYL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.031"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..673)
                     /gene="focB"
                     /locus_tag="ACHUOY_000907"
     CDS             complement(<1..673)
                     /gene="focB"
                     /locus_tag="ACHUOY_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR0681757.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="ACHUOY_000908"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="ACHUOY_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR0681758.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="ACHUOY_000909"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="ACHUOY_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR0681759.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="ACHUOY_000910"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="ACHUOY_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR0681760.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="ACHUOY_000911"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="ACHUOY_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR0681761.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="ACHUOY_000912"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="ACHUOY_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR0681762.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALSFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="ACHUOY_000913"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="ACHUOY_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR0681763.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="ACHUOY_000914"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="ACHUOY_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR0681764.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9837)
                     /gene="hyfD"
                     /locus_tag="ACHUOY_000915"
                     /pseudo
     CDS             complement(8399..9837)
                     /gene="hyfD"
                     /locus_tag="ACHUOY_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="ACHUOY_000916"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="ACHUOY_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR0681765.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="ACHUOY_000917"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="ACHUOY_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR0681766.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="ACHUOY_000918"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="ACHUOY_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR0681767.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="ACHUOY_000919"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="ACHUOY_000919"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR0681768.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="ACHUOY_000920"
     CDS             complement(14170..14742)
                     /locus_tag="ACHUOY_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR0681769.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="ACHUOY_000921"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="ACHUOY_000921"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR0681770.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="ACHUOY_000922"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="ACHUOY_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR0681771.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="ACHUOY_000923"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="ACHUOY_000923"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR0681772.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="ACHUOY_000924"
     CDS             17911..18759
                     /locus_tag="ACHUOY_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR0681773.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /gene="tmcA"
                     /locus_tag="ACHUOY_000925"
     CDS             18774..20789
                     /gene="tmcA"
                     /locus_tag="ACHUOY_000925"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR0681774.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="ACHUOY_000926"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="ACHUOY_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0681775.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="ACHUOY_000927"
     CDS             complement(21671..21871)
                     /locus_tag="ACHUOY_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR0681776.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="ACHUOY_000928"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="ACHUOY_000928"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR0681777.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="ACHUOY_000929"
     CDS             complement(23030..23386)
                     /locus_tag="ACHUOY_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR0681778.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5874 a         6227 c         6275 g         5083 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tagccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gctcagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggtgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattat
//
LOCUS       DAZOYL010000032        23252 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZOYL010000032 DAZOYL010000000
VERSION     DAZOYL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.032"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..543
                     /locus_tag="ACHUOY_000930"
     CDS             <1..543
                     /locus_tag="ACHUOY_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR0681779.1"
                     /translation="EVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSAA
                     VIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNAR
                     DSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNK
                     AAALDELIPGLLSEYIEQSG"
     gene            518..2443
                     /locus_tag="ACHUOY_000931"
     CDS             518..2443
                     /locus_tag="ACHUOY_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0681780.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2440..2646
                     /locus_tag="ACHUOY_000932"
     CDS             2440..2646
                     /locus_tag="ACHUOY_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR0681781.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2643..4244
                     /locus_tag="ACHUOY_000933"
     CDS             2643..4244
                     /locus_tag="ACHUOY_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0681782.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4225..5544
                     /locus_tag="ACHUOY_000934"
     CDS             4225..5544
                     /locus_tag="ACHUOY_000934"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0681783.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5554..5886
                     /locus_tag="ACHUOY_000935"
     CDS             5554..5886
                     /locus_tag="ACHUOY_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR0681784.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            5942..6967
                     /locus_tag="ACHUOY_000936"
     CDS             5942..6967
                     /locus_tag="ACHUOY_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0681785.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7009..7407
                     /locus_tag="ACHUOY_000937"
     CDS             7009..7407
                     /locus_tag="ACHUOY_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0681786.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            7419..7772
                     /locus_tag="ACHUOY_000938"
     CDS             7419..7772
                     /locus_tag="ACHUOY_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0681787.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7784..8362
                     /locus_tag="ACHUOY_000939"
     CDS             7784..8362
                     /locus_tag="ACHUOY_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0681788.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8359..8754
                     /locus_tag="ACHUOY_000940"
     CDS             8359..8754
                     /locus_tag="ACHUOY_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0681789.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            8762..9502
                     /locus_tag="ACHUOY_000941"
     CDS             8762..9502
                     /locus_tag="ACHUOY_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0681790.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            9518..9940
                     /locus_tag="ACHUOY_000942"
     CDS             9518..9940
                     /locus_tag="ACHUOY_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0681791.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            9922..10356
                     /locus_tag="ACHUOY_000943"
     CDS             9922..10356
                     /locus_tag="ACHUOY_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0681792.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10349..12910
                     /locus_tag="ACHUOY_000944"
     CDS             10349..12910
                     /locus_tag="ACHUOY_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0681793.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            12907..13236
                     /locus_tag="ACHUOY_000945"
     CDS             12907..13236
                     /locus_tag="ACHUOY_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0681794.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13236..13934
                     /locus_tag="ACHUOY_000946"
     CDS             13236..13934
                     /locus_tag="ACHUOY_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0681795.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            13940..14683
                     /locus_tag="ACHUOY_000947"
     CDS             13940..14683
                     /locus_tag="ACHUOY_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0681796.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            14680..15252
                     /locus_tag="ACHUOY_000948"
     CDS             14680..15252
                     /locus_tag="ACHUOY_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0681797.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15313..18810
                     /locus_tag="ACHUOY_000949"
     CDS             15313..18810
                     /locus_tag="ACHUOY_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR0681798.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            18881..19397
                     /locus_tag="ACHUOY_000950"
                     /pseudo
     CDS             18881..19397
                     /locus_tag="ACHUOY_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            19462..22545
                     /locus_tag="ACHUOY_000951"
     CDS             19462..22545
                     /locus_tag="ACHUOY_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0681799.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            22557..23126
                     /locus_tag="ACHUOY_000952"
     CDS             22557..23126
                     /locus_tag="ACHUOY_000952"
                     /inference="COORDINATES: protein motif:HMM:NF014467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0681800.1"
                     /translation="MSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTDIAPP
                     DIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSPEGEF
                     QKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKETLLL
                     EAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         5498 a         5878 c         7165 g         4711 t
ORIGIN      
        1 gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga gtatccgtac cattcagaac
       61 tggcaggaac agggaatgcc cgttctgcga ggcggtggca agggtaatga ggtgctttat
      121 gactctgccg ccgttataag atggtatgcc gaaagggatg ctgaaattga gaacgaaaag
      181 ctgcgccggg aagttgaaga actgcggcag gccagcgaga cagatctcca gccagggact
      241 attgagtacg aacgccatcg acttacgcgt gcgcaggccg acgcacagga gctgaaaaat
      301 gccagagact ccgctgaagt ggtggaaacc gcattctgta ctttcgtgct gtcgcggatc
      361 gcaggtgaaa ttgccagtat tctcgacggg atccccctgt cggtgcagcg gcgttttccg
      421 gaactggaaa accgacatgt tgatttcctg aaacgggata tcatcaaagc catgaacaaa
      481 gcagccgcgc tggatgaact gataccgggg ttgctgagtg aatatatcga acagtcaggt
      541 taacaggctg cggcattttg tccgcgccgg gcttcgctca ctgttcaggc cggagccaca
      601 gaccgccgtt gaatgggcgg atgctaatta ctatctcccg aaagaatccg cataccagga
      661 agggcgctgg gaaacactgc cctttcagcg ggccatcatg aatgcgatgg gcagcgacta
      721 catccgtgag gtgaatgtgg tgaagtctgc ccgtgtcggt tattccaaaa tgctgctggg
      781 tgtttatgcc tactttatag agcataagca gcgcaacaca cttatctggt tgccgacgga
      841 tggtgatgcc gagaacttta tgaaaaccca cgttgagccg accatccgcg atattccgtc
      901 gctgctggcg ctggctccgt ggtatggcaa aaagcaccgg gataacacgc tcactatgaa
      961 gcgtttttcc aatggtcgtg gcttctggtg cctgggcggt aaagcggcaa aaaactaccg
     1021 tgaaaagtcg gtggatgtgg cgggttatga tgaacttgct gcctttgatg aggatattga
     1081 acaggaaggc tctccgacgt tccttggcga caaacgtatt gaaggctcgg tctggccaaa
     1141 gtccatccgt ggctccacgc ccaaagtgag aggcacctgc cagattgagc gtgcagccag
     1201 tgaatccccg cattttatgc gttttcatgt tgcctgcccg cactgcgggg aggagcagta
     1261 tcttaaattt ggcgacaaag agacgccgtt tggcctcaaa tggacgccgg atgacccctc
     1321 cagcgtgttt tatctctgcg agcataatgc ctgcgtcatc cgccagcagg agctggactt
     1381 tactgatgcc cgttatatct gcgaaaagac cgggatctgg acccgtgatg gcattctctg
     1441 gttttcgtca tccggtgaag agattgagcc acctgacagt gtgacctttc acatctggac
     1501 agcgtacagc ccgttcacca cctgggtgca gattgtcaaa gactggatga aaacgaaagg
     1561 ggatacggga aaacgtaaaa ccttcgtaaa caccacgctc ggtgagacgt gggaggcgaa
     1621 aattggcgaa cgtccggatg ctgaagtgat ggcagagcgg aaagagcatt attcagcgcc
     1681 cgttcctgac cgtgtggctt acctgaccgc cggtatcgac tcccagcggg atcgctacga
     1741 aatgcgcgta tggggatggg ggccgggtga ggaaagctgg ctgattgatc ggcagattat
     1801 tatgggccgc cacgacgatg aacagacgct gctgcgtgtg gatgaggcca tcaataaaac
     1861 ctatacccgc cggaatggtg cagaaatgtc gatatcccgt atctgctggg atattggcgg
     1921 gattgacccg accattgtgt atgaacgctc gaaaaagcat gggctgttcc gggtgatccc
     1981 cattaaaggg gcatccgtct acggtaagcc ggtggccagc atgcctcgta agcgaaacaa
     2041 aaacggggtt taccttaccg aaatcggtac ggataccgcg aaagagcaga tttataaccg
     2101 cttcacactg acgccggaag gggatgaacc gcttcccggt gccgttcact tcccgaataa
     2161 cccggatatt tttgatctga ccgaagcgca gcagctgact gctgaagagc aggtcgaaaa
     2221 atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa aagcgacgca atgaggcgct
     2281 cgactgcttc gtttatgcgc tggcggcgct gcgcatcagt atttcccgct ggcagctgga
     2341 tctcagtgca ctgctggcga gcctgcagga agaggatggt gcagcaacca acaagaaaac
     2401 actggcagat tacgcccgtg ccttatccgg agaggatgaa tgacgcgaca ggaagaactt
     2461 gccgctgccc gtgcggcact gcatgacctg atgacaggta aacgggtggc aacggtacag
     2521 aaagacggac ggcgagtgga gtttacggcc acttccgtgt ctgacctgaa aaaatacatt
     2581 gcggagctgg aggtgcagac cggcatgaca cagcgacgca ggggacctgc aggattttat
     2641 gtatgaaaac gcccaccatt cccacccttc tgggaccgga cggcatgaca tcgctgcgtg
     2701 aatatgccgg ttatcacggc ggtggcagcg gatttggtgg gcagttgcgg gcgtggaacc
     2761 caccgggtga aagtgtggat gcagccctgc tgcccaactt tacccgtggc aatgcccgcg
     2821 cagacgatct ggtacgcaat aacggctatg ctgccaacgc catccagctg catcaggatc
     2881 atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat ctgggcatcg
     2941 gggaggaaga cgcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa gagtttgccg
     3001 aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg atgattcggg
     3061 aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc tgggatacca
     3121 gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc atcagcaacc
     3181 cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat gacagcggtg
     3241 cggcgctggg atattacgtc agcgaggacg gctatcctgg ctggatgccg cagaaatgga
     3301 catggatacc ccgtgagtta cccggcgggc gcgcctcgtt cattcacgtt tttgaacccg
     3361 tggaggacgg gcagacccgc ggtgcaaatg tgttttacag cgtaatggag cagatgaaga
     3421 tgctcgacac gctgcagaac acgcagctgc agagcgccat tgtgaaggcg atgtatgccg
     3481 ccaccattga aagtgagctg gatacgcagt cagcgatgga ttttattctg ggcgcgaaca
     3541 gtaaggagca gcgggaaagg ctgacgggct ggattggtga aattgccgcg tattacgccg
     3601 cagcaccggt ccgtctggga ggcgcaaaag tgccgcacct gatgccgggg gactcactga
     3661 acctgcagac ggctcaggac acggataacg gctactccgt gtttgagcag tcactgttgc
     3721 ggtatatcgc tgccgggctg ggtgtctcgt atgagcaact ttcccggaat tacgcccaga
     3781 tgagctactc cacggcacgg gccagtgcga acgagtcgtg ggcgtacttt atggggcggc
     3841 gaaaattcgt cgcatcccgt caggcgagcc agatgtttct gtgctggctg gaagaggcca
     3901 tcgttcgccg cgtggtgacg ttaccttcaa aagcgcgctt cagctttcag gaagcccgca
     3961 gtgcctgggg gaactgcgac tggataggct ccggtcgtat ggccatcgat ggtctgaaag
     4021 aagttcagga agcggtgatg ctgatagaag ccggactgag cacctacgag aaagagtgcg
     4081 cgaaacgcgg tgacgactat caggaaattt ttgcccagca ggtccgtgaa acgatggagc
     4141 gccgcgcagc tggtcttaaa ccgcccgcct gggcggctgc ggcatttgaa tccgggctgc
     4201 gacaatcaac agaggaggag aagagtgaca gcagagctgc gtaatctccc gcatattgcc
     4261 agcatggctt ttaatgagcc gctgatgctt gaacccgcct atgcgcgggt tttcttttgt
     4321 gcgcttgcag gccagcttgg gatcagctgc ctgacggatg cagtatccgg cgacagcctg
     4381 actgccggag aggcacccgc ggcgctggcg ttatccggtg atgatgacgg accacgacag
     4441 gcccgcagtt atcaggtaat gaacggcatc gccgtgctgc cggtgtccgg cacgctggtc
     4501 agccggacgc gggcgctgca gccgtactcg gggatgaccg gttacaacgg cattatcgcc
     4561 cgtctgcaac aggctgccag cgacccgatg gtggacggca ttctgctcga tatggacaca
     4621 ccgggcggaa tggtggcggg agcatttgac tgcgctgaca tcatcgcccg tgtgcgtgac
     4681 ataaagccgg tatgggcgct ggccaatgac atgaactgca gcgcaggtca gctgcttgcc
     4741 agtgccgcct cccggcgtct ggtcacgcag accgcccgga caggctccat cggcgtcatg
     4801 atggctcaca gtaattacgg cgctgcactg gagaaacagg gcgtggaaat cacgctgatt
     4861 tacagcggca gccataaggt ggatggcaac ccctacagcc atctaccgga tgatgtccgg
     4921 gaaacattgc agtcccggat ggatgcaacc cgccggatgt ttgcacagaa ggtgtcggca
     4981 tataccggcc tgtccgtgca ggctgtgctg gataccgagg ctgcagtgta cagcggtcag
     5041 gaggccattg atgccggact ggctgatgaa cttgttaaca gtaccgatgc gatcaccgtc
     5101 atgcgtgatg cactggatgc acgtaaatcc cgtctctcag gagggcgaat gaccaaagag
     5161 actcaatcaa caactgtttc agccactgct tcgcaggctg acgttactgg cgtggtgcaa
     5221 gcgacggagg gcgagaacgc cagcgcggcg cagccggacg tgaacgcgca gatcaccgca
     5281 gcggttgcgg cagaaaacag tcgcattatg gggatcctca actgtgagga ggctcacgga
     5341 cgcgaagaac aggcacgcgt gctggccgaa acccccggta tgaccgtgga aacggcccgc
     5401 cgtattctgg ccgcagcacc acagagtgca cagacgcgca gtgacactgc gctggatcgt
     5461 ctgatgcagg gggcaccggc accgctggct gcaggtaacc cggcatctga tgccgttaac
     5521 gatttgctga acacaccagt gtaagggatg tttatgacga gcaaagaaac ctttacccat
     5581 taccagccgc tgggcaatag tgacccggct cataccgcaa ccgcgcccgg cggattgagt
     5641 gcgaaagcgc ctgcaatgac cccgctgatg ctggacacct ccacccgtaa gctggttgcg
     5701 tgggatggca ccaccgacgg tgctgccgtt ggcattcttg cggttgctgc tgaccagacc
     5761 agcaccacac tgacgttcta caagtccggc acgttccgtt atgaggatgt gctctggccg
     5821 gaggctgcca gcgacgagac gaaaaaacgg accgtgtttg ccggaacggc aatcagcatc
     5881 gtttaacttt acccttcatc actaaaggcc gcctgtgtgg ctttttttac gggatttttt
     5941 tatgtcgatg tacacaaccg cccagctgct ggcggcaaat gagcagaaat ttaagtttga
     6001 tccgctgttt ctgcgtctct ttttccgtga gagctatccc ttcactacgg agaaagtcta
     6061 tctctcacaa attccgggac tggtaaacat ggcgctgtac gtttcgccga ttgtttccgg
     6121 tgaggttatc cgttcccgtg gcggctccac ctctgaattt acgccgggat atgtcaaacc
     6181 caagcatgag gtgaatccgc agatgaccct gcgtcgcctg ccggatgaag atccgcagaa
     6241 tctggcggac ccggcttacc gccgccgtcg catcatcatg cagaacatgc gtgacgaaga
     6301 gctggccatt gctcaggtcg aagagatgca ggcagtttct gccgtgctca agggcaaata
     6361 caccatgacc ggtgaagcct tcgatccggt tgaggtggat atgggccgca gtgcggcgaa
     6421 caacatcaca cagtccggcg tcacggagtg gagcaagcgt gacaagtcca cgtatgaccc
     6481 gaccgacgat atcgaagcct acgcgctgaa cgccagcggt gtggtgaata tcatcgtgtt
     6541 tgacccgaaa ggctgggcgc tgttccgttc cttcaaagcc gtcaaggaga agctggatac
     6601 ccgtcgcggc tctcattccg agctggagac agcggtaaaa gacctgggca aagcggtgtc
     6661 ctataaggga atgtatggcg atgtggccat cgtcgtgtat tccggacagt acgtggaaaa
     6721 cggcgtcaaa aagaacttcc tgccggacaa cacgatggtg ctggggaaca ctcaggctcg
     6781 cggtctgcgc acctatggct gcattcagga tgcggacgca cagcgcgaag gcattaacgc
     6841 ttctgcccgt tacccgaaaa actgggtgac caccggcgat ccggcgcgtg agttcaccat
     6901 gattcagtca gcaccgctga tgctgctggc tgatcctgat gagttcgtgt ccgttcaact
     6961 ggcgtaatca tggcccttcg gggccatttt ctctctgtgg aggagttcat gacgaaagat
     7021 gaactgattg cccgtcttca ggagctgggt gagcaattga accgcgatgt cagtttgacg
     7081 ggaacgaaag aagaactggc gcttcgtgtg gcagagctgg aagaagagct tgatgacacg
     7141 gatgaaactg ccggtcagga cacccctctc agccgggaaa atgtgctgac cgggcatgaa
     7201 aatgaggtgg tatcagcgca gccggatacc gtgattcagg atacggctga actggtcacg
     7261 gtcgtggcac tggtgacgct gcatactgat gcacttcacg ccacgcggga taaacctgtg
     7321 gcatttgtgc tgccgggaac ggcgtttcgt gtctctgccg gtgtggcagc cgaaatgaca
     7381 gagcgcggcc tggccagaat gcaataacgg gaggcgctgt ggctgatttc gataacctgt
     7441 tcgatgctgc cattgcccgc gccgatgaaa cgatacgcgg gtacatggga acgtcagcca
     7501 ccatgacatc cggtgagcag tcaggtgcgg tgatacgtgg tgtttttgat gaccctgaaa
     7561 atatcagcta tgccggacag ggcgtgcgcg ttgaaggctc cagcccgtcc ctgtttgtcc
     7621 ggactgatga ggtgcggcag ctgcggcgtg gagacacgct gaccatcggt gaggaaaatt
     7681 tctgggtaga tcgggtttcg ccggatgatg gcggaagttg tcatctctgg cttggacggg
     7741 gcgtaccgcc tgccgttaac cgtcgccgct gaaaggggga tgtatggcca taaaaggtct
     7801 tgagcaggcc gttgaaaacc tcagccgtat cagcaaaacg gcggtgcccg gtgccgccgc
     7861 aatggccatt aaccgcgttg cttcatccgc gatatcgcag tctgcgtcac aggttgcccg
     7921 tgagacaaag gtacgccgga aactggtaaa ggaaagggcc aggctgaaaa gggccacggt
     7981 caaaaatccg caggccagaa tcaaagttaa ccggggggat ttgcccgtaa tcaagctggg
     8041 taatgcgcgg gttgtcctgt cccgccgcag gcgtcataaa aaggggcagc gttcatccct
     8101 gaaaggtggc ggcagcgtgc ttgtggtggg taaccgtcgt attcccggcg cgtttattca
     8161 gcaactgaaa aacggccggt ggcatgttat gcagcgtgtg gccgggaaaa accgttaccc
     8221 cattgatgtg gtgaaaatcc cgatggcggt gccgctgacc acggcgttta aacagaatat
     8281 tgaacggata cggcgtgaac gtcttccgaa agagctgggc tatgcgctgc agcatcaact
     8341 gagaatggta ataaagcgat gaaacatact gaactccgtg cagccgtact ggatgcactg
     8401 gagaagcatg acaccggggc gacgcttttt gatggtcgcc ccgctgtttt tgatgaggaa
     8461 gattttccgg caattgccgt ttatctcacc ggcgctgaat acacgggcga agagctggac
     8521 agcgatacct ggcaggcgga gctgcatatt gaagttttcc tgcctgctca ggtgccggat
     8581 tcagagctgg attcgtggat ggagtcccgg atttatccgg tgatgagcga tatcccggca
     8641 ctgtcagatt tgatcaccag tatggtggcc agcggctatg actaccggcg cgacgatgat
     8701 gcgggcctgt ggagttcagc cgatctgact tatgtcatta tctatgaaat gtgaggacgc
     8761 tatgcctgta ccaaatccta caatgccggt gaaaggtgcc gggaccaccc tgtgggttta
     8821 taaggggagc ggtgaccctt atgcgaaccc gctttcagac gttgactggt cgcgtctggc
     8881 aaaagttaaa gacctgacgc ccggcgaact gaccgctgag tcctatgacg acagctatct
     8941 cgatgatgaa gatgcagact ggactgcgac cgggcagggg cagaaatctg ccggagatac
     9001 cagcttcacg ctggcgtgga tgcccggaga gcaggggcag caggcgctgc tggcgtggtt
     9061 taatgaaggt gatacccgtg cctataaaat ccgcttcccg aacggcacgg tcgatgtgtt
     9121 ccgtggctgg gtcagcagta tcggtaaggc ggtgacggcg aaggaggtga ttacccgcac
     9181 ggtgaaggtc accaatgtgg gacgtccgtc gatggcagaa gatcgcagca cggtgacggc
     9241 gacgaccggc atgacggtga cacccgccag tgcttccgta gtgaaagggc agagcaccac
     9301 gctgaccgtg gcattccagc cggatggcgc aaccgacaag agcttccgtg cggtgtctgc
     9361 ggataaaaca aaagccaccg tgtcggtcag tggtatgacc atcaccgtga aaggtgttgc
     9421 tgcaggcaag gtcaacattc cggtcgtatc tggtaatggt gagtttgctg cggttgcaga
     9481 aatcaacgtc accgccagtt aatccggaga gtcagcgatg ttcctgaaaa ccgaatcatt
     9541 tgaacataac ggtgtgaccg tcacgctttc tgaactgtca gccctgcagc gtattgagca
     9601 tctcgacctg atgaaacggc aggcagaaca ggcggagtca gacagcaacc ggaagtttac
     9661 tgtggaagac gccatcagaa ccggcgcttt tgtggttgcg atgtccctgt ggcataacca
     9721 tccgaagaag acgcagatgc cgtccatgaa tgaagccgtt aaacagattg agcaggaagt
     9781 gcttaccacc tggcccacag aggcaatttc tcatgctgaa aacgtggtgt accggctgtc
     9841 cggtatgtat gagtttgtgg tgaatgatgc tcctgaacag gcagaggacg ccgggcctgc
     9901 agagcctgtt tctgcgggaa agtgttcgac ggtgagctga gttttgccct gaaactggcg
     9961 cgtgagatgg ggcgacccga ctggcgcgcc atgcttgccg ggatgtcatc cacggagtat
    10021 gccgactggc accgctttta cagtacccat tattttcatg atgttctgct ggatatgcac
    10081 ttttccgggc tgacgtacac cgtgctcagc ctgtttttca gcgatccgga gatgcatccg
    10141 ctggatttca gtctgctgaa ccggcgcgag gctgacgaag agcctgaaga tgatgtgctg
    10201 atgcagaaag cggcagggct tgccggaggt gtccgctttg gcccggacgg gaatgaagtt
    10261 atccccgctt ccccggatgt ggcggacatg acggaggatg acgtaatgct gatgacagta
    10321 tcagaaggga tcgcaggagg agtccggtat ggctgaaccg gtaggcgatc tggtcgttga
    10381 tttgagtctg gatgcggcca gatttgacga gcagatggcc agagtcaggc gtcatttttc
    10441 cggtacggaa agtgatgcga aaaaaacagc ggcagtcgtt gaacagtcgc tgagccgaca
    10501 ggcgctggct gcacagaaag cggggatttc cgtcgggcag tataaagccg ccatgcgtat
    10561 gctgcctgca cagttcaccg acgtggccac gcagcttgca ggggggcaga atccctggct
    10621 gatcctgctg caacagggtg gtcaggttaa ggactccttc ggcgggatga tccccatgtt
    10681 cagggggctt gccggcgcga tcaccctgcc gatggtgggg gccacctcgc tggcggtggc
    10741 gaccggtgcg ctggcgtatg cctggtatca gggcaactca accctgtccg atttcaacaa
    10801 aacgctggtc ctttccggta atcaggcggg actgacggca gatcgtatgc tggtcctgtc
    10861 cagagccggg caggcggcag ggctgacgtt taaccagacc agcgagtcac tcagcgcact
    10921 ggttaaggcg ggggtaagcg gtgaggctca gattgcgtcc atcagccaga gtgtggcgcg
    10981 tttctcctat gcatccggcg tggaggtgga caaggtcgct gaagccttcg ggaagctgac
    11041 cacagacccg acgtcggggc tgacggcgat ggcacgccag ttccataacg tgacggcgga
    11101 gcagattgcg tatgttgctc agttgcagcg ttccggcgat gaagccgggg cattgcaggc
    11161 ggcgaacgag gccgcaacga aagggtttga tgaccagacc cgccgcctga aagagaacat
    11221 gggcacgctg gagacctggg cagacaggac tgcacgtgca ttcaaatcca tgtgggatgc
    11281 ggtgctggat attggtcgtc ctgataccgc gcaggagatg ctgattaagg cagaggctgc
    11341 gtttaagaaa gcagacgaca tctggaatct gcgcaaggat gattatttcg ttaacgatga
    11401 agcacgggcg cgttactggg atgatcgtga aaaaaaacgc ctggagcggg atgcagccca
    11461 aaagagggta gatcaacagc gtcaacagga caaaaatgcg cagcagcaga gcgataccga
    11521 agcgtcacgg ctgaaatata ccgaagaggc gcagaaggct tacgaacggc tgcagacgcc
    11581 gctggagaaa tataccgccc gtcaggaaga actgaacaag gcactgaaag acggaaaaat
    11641 cctgcaggca gattacaaca cgctgatggc ggcggcgaaa aaggattatg aagcgacgct
    11701 gaaaaagccg aaacagtccg gcgtgaaggt gtatgcgggc gatcgtcagg aagacagtgc
    11761 tcatgcggcc ctgctgacgc ttcaggcaga actccggacg ctggagaagc atgccggagc
    11821 gaatgagaaa atcagccagc agcgccggga tttgtggaag gcagaaagtc agttcgcggt
    11881 actggaggag gcggcacaac gtcgccagct gtccgcacag gagaaatccc tgctggcgca
    11941 taaagacgag acgctggagt acaaacgcca gctggctgca cttggtgaca aggttacgta
    12001 tcaggagcac ctgaatgcgc tggcgcagca ggcggataaa ttcgcacagc agcaacgggc
    12061 aaaacgggca gccattgatg cgaaaaaccg ggggctgact gaccggcagg cagcgcggga
    12121 agccacggaa cagcgcctga aggaacagta tggcgataat cctctggcgc tgaataacgt
    12181 catgtcagag cagaaaaaga cctgggcggc tgaagaccag cttcgcggga gctggatggc
    12241 aggcctgaag tccggctgga gtgagtggga agagagcgcc acggacagta tgtcacaggt
    12301 taaaagtgca gccacgcaga cctttgatgg tattgcacag aatatggcgg cgatgctgac
    12361 cggcagtgaa cagaactggc gcagcttcac ccgctccgtg ctgtccatga tgacagaaat
    12421 tctgcttaag caggcaatgg tggggattgt cgggagtatc ggcagcgcca ttggcggggc
    12481 tgttggtggc ggcgcatccg cgtcaggtgg tacagccatt caggccgctg cggtgaaatt
    12541 ccattttgca accgggggat ttacgggaac cggcggcaaa tatgagccag cggggattgt
    12601 tcaccgtggt gaatttgtct tcacgaagga ggcaaccagc cggattggcg tggggaatct
    12661 ctaccggctg atgcgcggct atgcggaagg tggttatgtg ggcggtgccg gaagtccggc
    12721 gcagatgcgg cgggctgaag gcattaattt taatcagaac aatcacgtgg tgattcagaa
    12781 cgacggtacg aatggtctgc caggtccaca gatgatgaag gccgtgtatg acatggcccg
    12841 caagggtgcc cgtgatgaaa ttcagacaca gatgcgtgat ggtggcctgt tctccggagg
    12901 tggacgatga aaaccttccg ctggaaagtg aaacccggta tggatgtggc ttcggcccct
    12961 tctgtaagaa aggtgcgctt tggtgatggc tattcccagc gagcgcctgc cgggctgaat
    13021 gccaacctga aaacgtacag cgtgacgctt tctgtccccc gtgaggaggc catggtactg
    13081 gagtcgtttc tggaagagca cgggggctgg aaagcctttc tgtggacgcc gccttatgag
    13141 tggcggcaga taaaggtgac ctgcgcaaaa tggtcgtcgc gggtcagtat gctgcgtgtt
    13201 gagttcagcg cagagtttga acaggtggtg aactgatgca ggatatccgg caggaaacac
    13261 tgaatgaatg cacccgtgcg gagcagtcgg ccagcgtggt gctctgggaa atcgatctga
    13321 cagaggtcgg tggagaacgt tattttttct gtaatgagca gaacgaaaaa ggtgagccgg
    13381 tcacctggca ggggcgacag tatcagccgt atcccattca ggggagtggt tttgaactga
    13441 atggcaaagg caccagtacg cgccccacgc tgatggtttc taacctgtac ggtatggtca
    13501 ccgggatggc ggaagatcta cagagtctgg tcggcggaac ggtggtccgg cgtaaggttt
    13561 acgcccgttt tctggatgcg gtgaacttcg tcaacggaaa cagtgacgcc gatccggagc
    13621 aggaggtgat cagccgctgg cgcattgagc agtgcagcga actgagcgcg gtgagtgcct
    13681 cttttgtact gtccacgccg acggaaacag atggcgctgt ttttccggga cgtatcatgc
    13741 tggccaacac ctgcacctgg acctatcgcg gcgatgagtg cggttatcac ggtccggcgg
    13801 tcgcggatga atatgaccag ccaacgtccg acatcacgaa ggataaatgc agcaaatgcc
    13861 tgagtggctg taagtttcgc aataacgtcg gcaactttgg cggcttcctt tccattaaca
    13921 aactttcgca gtaaatccca tgacagagac agaatcagcg attctggcgc acgcccggcg
    13981 atgtgctcca gcggagtcgt gcggcttcgt ggtaagaacg ccggaggggg aaagatattt
    14041 cccctgcgtg aatatctccg gtgagccgga ggcgtatttc cgtatgtcgc cggaagactg
    14101 gctgcaggca gagatgcagg gtgagattgt ggcactggtc cacagccacc ccggtggtct
    14161 gccctggctg agtgaggccg accggcggct gcaggtgcag agtgatttgc cgtggtggct
    14221 ggtctgccgg ggggcgattc acaagttccg ctgtgtgcca catcttaccg ggcggcgctt
    14281 tgagcacggg gtgacggact gttacacgct gttccgggat gcttaccatc tggcggggat
    14341 tgagatgccg gattttcatc gtgaggatga ctggtggcgt cacggtcaga atctctatct
    14401 ggataatctg gaggccacag ggctgtatca ggtgccgttg tcagcggcgc agccgggcga
    14461 tgtgctgctg tgctgttttg gttcatcggt gccgaatcat gccgccattt actgtggtga
    14521 cggcgagctg ctgcaccata ttcctgaaca actgagcaaa cgagagaggt ataccgacaa
    14581 atggcagcga cgcacacact ccctctggcg tcaccgggca tggcacgcat ctgcctttac
    14641 ggggatttgc aacgatttgg ccgccgcatc gaccttcgtg tgaaaacggg tgccgaagcc
    14701 atccgggcga tggccacaca gctccctgcg tttcgtcaga agctgagcga cggctggtat
    14761 caggtgcgta ttgccgggca ggatgttagc acgtccggat taacggcaca gttacatgag
    14821 gctctgcctg acggcgctgt gattcatatt gttcccagag tcgccggggc caagtcaggt
    14881 ggtgtattcc agattgtcct gggagcagcc gccattgccg gatcattctt taccgccgga
    14941 gccacccttg cagcatgggg ggctgccatt ggtgccggtg gtatgaccgg cattctgttt
    15001 tctctcggtg ccagtatggt gctcggcggt gtggctcaga tgctggcacc gaaagccaga
    15061 actccccgca cacagacaac ggataacggt aagcagaaca cctatttctc ctcactggat
    15121 aacatggttg cccagggcaa tgttatgcct gttctgtatg gtgaaatgcg cgtggggtca
    15181 cgtgtggtat ctcaggagat cagcacggca gacgaagggg acggtggtca ggttgtggtg
    15241 attggtcgct gatgcaaaat gttttatgtg aaatcgcctc cgggcggttt tatcgtttat
    15301 ggagcatgac gaatgggtaa aggcagcagt aaggggcata ccccgcgcga agcgaaggac
    15361 aacctgaagt ccacgcagct gctgagtgtg atcgatgcca tcagcgaagg gccggttgaa
    15421 ggtccggtgg atggattaaa aagcgtgctg ctgaacagta cgccggtgct ggacagtgag
    15481 gggaatacca acatctccgg cgtcacggtg gtgttccggg caggtgagca ggagcagtca
    15541 ccgccggagg gatttgaatc ctccggctcc gagacggtgc tgggtacgga agtgaaatat
    15601 gacacgccga tcacccggac catcacgtcg gcaaacattg accgactgcg ttttaccttc
    15661 ggtgtacagg cactggtgga aaccacctca aagggagaca ggaatccgtc tgaagttcgc
    15721 ctgctggttc agatacagcg taacggtggc tgggtgacgg aaaaagacat caccattaag
    15781 ggcaaaacca cctcgcagta tctggcctcg gtggtggtgg ataacctgcc gccgcgcccg
    15841 tttaatatcc ggatgcgcag aatgacgccg gacagcacca cagaccagct gcagaacaaa
    15901 acgctctggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggca
    15961 ctggtcggcg tacaggtgga ttcggagcag ttcggcagcc agcaggtgag ccgtaattat
    16021 catcttcgcg ggcgcattct gcaggtgccg tcgaactata atccgcagac gcggcaatac
    16081 agcggtatct gggacggaac gttaaaaccg gcatacagca acaacatggc ctggtgtctg
    16141 tgggatatgc tgacccatcc gcgctatggc atggggaaac gtcttggtgc ggcagatgtg
    16201 gataaatggg cgctgtatgt catcggccag tactgtgacc agtcggtgcc ggacggcttt
    16261 ggcagcacgg agccgcgcat cacctgtaat gcctggctga ccacacagcg caaggcgtgg
    16321 gatgtgctca gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa cgggcagacg
    16381 ctgacgttcg tgcaggaccg acagtcggat aaggtgtggg cctataaccg cagtaatgtg
    16441 gtgatgccgg atgatggcgc gccgttccgc tacagtttca gcgccctgaa agaccgccat
    16501 aatgccgttg aggtgaactg gattgacccg gataacggct gggagacggc gacagagctt
    16561 gtggaggata cgcaggccat tgtccgttac ggtcgtaacg tcacgaagat ggatgccttt
    16621 ggctgtacca gccgggggca ggcacaccgc gccgggctgt ggctgattaa aacggaactg
    16681 ctggagacgc agaccgtgga tttcagcgtg ggtgctgaag ggcttcgtca tgtaccgggc
    16741 gatgtcattg aaatctgcga tgatgactat gccggtatcc gcaccggcgg gcgcgtgctg
    16801 gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg aaatcacgct gccatcctcc
    16861 ggcaccacgc tgataagcct ggttgacggg caggggaatc cggtcagcgt ggaggtccag
    16921 tccgtcaccg acggcgtgaa ggtaaaagtg agccgtgttc ctgacggtgt tgctgaatac
    16981 agcgtgtggg ggctgaagct gccgacgctg caccagcgat tgttccgctg cgtgagtatc
    17041 cgtgagaacg acgacggcac gtatgccatc accgccgtgc agcatgtgcc ggaaaaagaa
    17101 gccatcgtgg ataacggggc gcactttgac ggcgaccaga gcggcacggt gaatggtgtc
    17161 acgccgccag cggtgcagca cctgactgcc gaagtcaccg cagacagcgg ggaatatcag
    17221 gtgctggcgc gctgggacac gccgaaggtg gtgaagggcg tgagcttcct gctccgtctg
    17281 accgtaacag cggatgacgg cagtgagcgg ctggtcagca cggcccggac gacggaaacc
    17341 acttaccgct tcacacaact ggcgctgggg aactacaggc tgacagtccg ggcagtaaat
    17401 gcgtgggggc agcagggcga tccggcgtcg gtatcgttcc ggattgccgc accggcagcg
    17461 ctgtcgcgga ttgagctgac gccgggctat tttcagataa ccgccacgcc gcatcttgcg
    17521 gtttatgatc cgacggtaca gtttgagttc tggttctcgg aaaagcggat tgcggatatc
    17581 aggcaggttg aaaccacagc acgctatctt ggcacggcgc tgtactggat agccgccagt
    17641 atcaatatca aaccgggcca tgattattac ttttatatcc gcagtgtgaa caccgttggc
    17701 aaatcggcat tcgtggaggc tgttggtcag ccgagtgatg atgcatccgg ttatctggat
    17761 tttttcaaag gcgagatagg gaaaacccat ctggctcagg agctgtggac gcagattgat
    17821 aacggtcagc ttgcgcctga cctggctgaa atcaggacgt ccattacgga tgtcagcaat
    17881 gaaatcacgc agaccgtcaa taagaaactg gaagaccaga gtgcggcaat tcagcagata
    17941 cagaaggttc aggttgatac aaataataac ctgaacagca tgtgggctgt gaagctgcag
    18001 cagatgcagg acggacgcct ttatatcgcg ggtattggtg ccggtattga gaacacccct
    18061 gacggcatgc agagtcaggt gctgctggcg gcggacagga ttgcgatggt taatcctgcg
    18121 aatggcaaca caaaaccgat gtttgttggt cagggcgatc agatattcat gaacgacgtg
    18181 ttcctgaaac gcctgacggc ccccaccatt accagcggtg gaaatccatc ggcattttcc
    18241 ctgactccgg acggaaagct gaccgctaaa aatgcggata tcagtggcag tgtgaatgcg
    18301 aacgccggga cgctcaacaa tgtcacggta aatgaaaact gtacgattaa gggcatgctg
    18361 gaggcgactc aggtcagagg tgacttcgtt aaagctgtat ccaaatcatt tccgaaacag
    18421 gctggtacgt ggggtaacac ggaaacacca aacgggacgg ttacagtcac cataagcgat
    18481 gatcataact ttgaccgcca gattattatt ccgcccatta tttttaacgg tatagcgtat
    18541 gacgatccgg ggagcggaaa taacccagga ggcacgcgat acacgggtta tggttttgaa
    18601 gttcgcaaaa acggcgtatt aatcgcatcc agagaaacta aaggtgccat tcccggtagt
    18661 tacagtgcag ttattgatat gcctagtggt ggtggtagcg tcactctgga gtttaagatt
    18721 ttccagaaag gcaatcaggg ggcaggcaat atcaccgact gtacggtgat tgtgaccaaa
    18781 aaagcggctt ccggcatcag tattcgttga aatatttata accccaataa atggcgtcag
    18841 gaatgacgcc ttttttattg cagaaaagcg agaggtaatt atgcgtaaag tttgtgcagc
    18901 cattttgtcc gcagccattt gcctggccgt atccggtgcg cctgcatggg cgtctgaaca
    18961 tcagtccacg ctgagcgcgg ggtatcttca tgcccggacc aacgttcccg gcagtgatga
    19021 tctgaacggg attaacgtga aataccgtta tgagtttacg gatacgctgg ggctggtgac
    19081 gtcattcagc tatgcaggag acaagaatcg ccagctgacc cgttacagcg atacccgctg
    19141 gcatgaagat tccgtgcgta accgctggtt cagcgtgatg gcggggccgt ctgtgcgcgt
    19201 gaatgaatgg ttcagcgcgt atgcgatggc gggtgtggct tacagccgtg tgtcgacttt
    19261 ttccggggat tatatccgcg taactgacaa taaggggaaa acgcacgatg tgctgaccgg
    19321 aagtgatgac ggtcgccaca gcaacacgtc tctggcgcac tgacggtttc atcgtgggtg
    19381 tcggttataa attctgatta gccaggtaac acagtgttat gacagcccgc cggttcaggc
    19441 gggctttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa agacggcaca
    19501 ggaaaaccgg tacagaactg cacaatccag ctgaaagcaa aacgtaacag caccacggtg
    19561 gtggtgaaca cgctggcctc agaaaatccg gatgaagccg ggcgttacag catggacgtt
    19621 gagtacggtc agtacagcgt tattctgttg gtggaaggat tcccgccgtc acatgccggg
    19681 accatcaccg tgtatgaaga ttcccgaccc ggtacgctga atgattttct cggtgccatg
    19741 acggaggatg atgcccgtcc ggaggcactg cgccgttttg agctgatggt ggaagaggtg
    19801 gcgcgtaacg cgtccgcggt ggcacagaac acggcagccg cgaagaaatc agccagcgat
    19861 gccagcacat cagcccgtga ggcggcaacc catgcgactg atgctgcaga ttcagcacgt
    19921 gcagccagca cgtcagccgg acaggccgct tcgtcggctc agtcagcgtc ttccagcgca
    19981 ggaacggcat caacaaaggc cactgaagcc tcaaaaagtg ctgccgctgc agagtcctca
    20041 aaaagcgcgg cggctaccag tgccggtgcg gcgaaaacgt tagaaacgaa tgcggcagcg
    20101 tcacaacaat cagcagccac ttctgcatcc accgcgacca cgaaagcgtc agaagctgcc
    20161 acctcagccc gggatgcgtc ggcttcaaaa gaggcggcaa aatcatcaga aacgaacgca
    20221 tcatcaagcg ccagtagcgc agcttcctcg gcgacggcgg ctgcaaattc tgcgaaggcg
    20281 gccaaaacgt ccgagacgaa cgccaggtct tctgaaacgg cagcgggaca gagtgcctca
    20341 gctgcggcag gctcaaaaac agcggctgcg tcgtctgcca gcgccgcgtc aacaagtgcc
    20401 gggcaggcct cagccagtgc caccgccgcc ggaaaatcgg cagaaagcgc cgcatcatcc
    20461 gcttcaacag ccacaacgaa ggctggcgaa gccactgagc aggccacggc agcagcgagg
    20521 tcagcttccg cagcgaagac atccgaaacg aacgcgaaag cgtcggaaac ccgtgcagaa
    20581 tcctcaaaaa cggctgccgc atcgtccgcc agttcggcgg cgtcatcggc atcatcggcg
    20641 tctgcttcaa aagatgaggc gaccagacag gcgtcagcag cgaagggcag cgccacgacg
    20701 gcatccacga aggcgacaga ggctgctggt agtgcgacgg cagcagctca gagcaaaagt
    20761 acggcggaat ctgcagcaac gcgcgctgag acagcggcaa aacgggcaga ggatattgca
    20821 tccgccgtgg cgcttgagga tgcgagcacg acgaaaaagg ggatagtaca gctcagtagt
    20881 gcgactaaca gcacttccga gtcactggcg gcaacaccaa aagccgttaa ggccgcgtat
    20941 gacctggcta acgggaaata caccgcacag gatgcaacga cagcacagaa agggataatc
    21001 cagctaagca gcgcgaccaa cagcacgtct gaaacgctgg cggcaacgcc aaaggcagta
    21061 aaagcagcca atgacaatgc tgagaaacgt ctgcagaaag atcagaacgg tgcggatatc
    21121 cctggcaaag acacctttac gaaaaatatt ggtgcctgcc gtgccttcgg tgggtcagta
    21181 agcacaacaa caggaaactg gacgactgca cagtttatcg agtggctgga ttctcaggga
    21241 gcatttaacc atccatactg gatgtgcaag ggttcctggt cttatggcaa taataaaatc
    21301 attactgata ctggctgcgg taatattcat ctcgccggag ctgtcattga agtaatgggg
    21361 ataaagtcag cgatgacgat ccgcattacc actccgacca catccagcgg tggcggaaca
    21421 accaacgccc agtttaccta tattaatcac ggaacagatt attcacctgg ctggcgaagg
    21481 gactataact ccagaaataa gccaacggca tcagagatcg gggcgctacc gtcaggtgga
    21541 acagcagtat catcagttaa tctggcttca aaaggtcggg tagccgcgct gacagacaat
    21601 acgcaggggg cagcaggtct tgagttatac gaggtgtata acaacggata tccaacagcg
    21661 tatggaaata tcattcacct gaaagggatg acagccgttg gcgaaggtga gttactcatc
    21721 ggctggagtg gtacaagcgg tgctcatgct ccggcattta ttcgttcacg acgggatacg
    21781 accgacgcaa actggtcgcc gtgggcgcag ctttacacct cggctcatcc tcctgcagag
    21841 ttttatccag tcggtgcacc aatcccgtgg ccatcagata ccgttccgtc tggttatgcc
    21901 ctgatgcagg ggcagacttt tgacaaatct gcttacccga aacttgcagc cgcttatccg
    21961 tcaggcgtga tccctgatat gcgtggctgg acgattaagg gcaagcccgc cagtggtcgt
    22021 gccgtattgt ctcaggaaca ggatggcatt aaatcgcaca cccacagcgc cagcgcatcc
    22081 agtacggatt tggggacgaa aaccacatcg tcgtttgatt acggcactaa atccacgaat
    22141 aacaccgggg cgcatacgca cagtctgagc ggctctacaa gttcagctgg ggcccaccaa
    22201 catagtcaga ctgggcccag aacgaatagc gggagtcaac ctactggaat gtttccggca
    22261 gggagtactc aagttagcgg gactaatcag gttggtatca gtggctcatt aaccagtggc
    22321 acctcccaat gggtaggtaa atcatcttca gaaggaaacc atacacactc actgtccggc
    22381 acagcagcat ctgcgggtgc acacgcgcat actgtcggta ttggtgctca tacgcactct
    22441 gttgcgattg gttcacatgg acacaccatc accgttaacg ctgctggtaa cgcggaaaac
    22501 accgtcaaaa acatcgcatt taactatatt gtgaggctcg cataatggca ttcagaatga
    22561 gtgaacaagc acggaccata aaaatttata acctgctggc cggaactaat gaatttattg
    22621 gtgaaggtga cgcatatatt ccgcctcata caggtctgcc tgcaaacagt accgatattg
    22681 caccgccaga tattccggct ggtttcgtgg ctgttttcaa cagtgatgag tcatcgtggc
    22741 atctcgttga agatcatcgg ggtaaaacgg tttatgacgt ggcttccggc gacgcgttat
    22801 ttatttctga actcggctca ttaccggaaa atgtcacctg gttatccccg gaaggggagt
    22861 ttcagaagtg gaacggcaca gcctgggtga aggatacgga agcagaaaaa ctgttccgga
    22921 tccgggaggc ggaagaaaca aaaaacagcc tgatgcaggt agccagtgag catatagcgc
    22981 cgcttcagga tgctgtggat ctggagatcg caacggagaa agaaaccttg ttgctggaag
    23041 cttggaaaaa gtatcgggtg ttgctgaacc gtgttgatac atcaactgca ccggatattg
    23101 aatggccagt agcacctata gggtaaattc gtaatgatta cctaaatacg ttattctttt
    23161 gttaaaaaag tgattttgct ataggtaggg agtatgttct tggattaaga ataaaaaatc
    23221 gccatgaaag agcttcatgg cgagtacata aa
//
LOCUS       DAZOYL010000033        22772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZOYL010000033 DAZOYL010000000
VERSION     DAZOYL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.033"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            44..673
                     /locus_tag="ACHUOY_000953"
     CDS             44..673
                     /locus_tag="ACHUOY_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR0681801.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            700..2361
                     /locus_tag="ACHUOY_000954"
     CDS             700..2361
                     /locus_tag="ACHUOY_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR0681802.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            2600..2659
                     /locus_tag="ACHUOY_000955"
     CDS             2600..2659
                     /locus_tag="ACHUOY_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0681803.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2976..3035
                     /gene="ibsE"
                     /locus_tag="ACHUOY_000956"
     CDS             2976..3035
                     /gene="ibsE"
                     /locus_tag="ACHUOY_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR0681804.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3155..4588)
                     /gene="hldE"
                     /locus_tag="ACHUOY_000957"
     CDS             complement(3155..4588)
                     /gene="hldE"
                     /locus_tag="ACHUOY_000957"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR0681805.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4636..7476)
                     /gene="glnE"
                     /locus_tag="ACHUOY_000958"
     CDS             complement(4636..7476)
                     /gene="glnE"
                     /locus_tag="ACHUOY_000958"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR0681806.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7499..8800)
                     /gene="ygiF"
                     /locus_tag="ACHUOY_000959"
     CDS             complement(7499..8800)
                     /gene="ygiF"
                     /locus_tag="ACHUOY_000959"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR0681807.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9042..9662
                     /locus_tag="ACHUOY_000960"
     CDS             9042..9662
                     /locus_tag="ACHUOY_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR0681808.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9726..10964
                     /gene="cca"
                     /locus_tag="ACHUOY_000961"
     CDS             9726..10964
                     /gene="cca"
                     /locus_tag="ACHUOY_000961"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR0681809.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11127..11948)
                     /gene="bacA"
                     /locus_tag="ACHUOY_000962"
     CDS             complement(11127..11948)
                     /gene="bacA"
                     /locus_tag="ACHUOY_000962"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0681810.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12039..12407)
                     /gene="folB"
                     /locus_tag="ACHUOY_000963"
     CDS             complement(12039..12407)
                     /gene="folB"
                     /locus_tag="ACHUOY_000963"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR0681811.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12512..13129
                     /gene="plsY"
                     /locus_tag="ACHUOY_000964"
     CDS             12512..13129
                     /gene="plsY"
                     /locus_tag="ACHUOY_000964"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR0681812.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13142..14074)
                     /gene="ttdR"
                     /locus_tag="ACHUOY_000965"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13142..14074)
                     /gene="ttdR"
                     /locus_tag="ACHUOY_000965"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
     gene            14281..15192
                     /gene="ttdA"
                     /locus_tag="ACHUOY_000966"
     CDS             14281..15192
                     /gene="ttdA"
                     /locus_tag="ACHUOY_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR0681813.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15189..15794
                     /gene="ttdB"
                     /locus_tag="ACHUOY_000967"
     CDS             15189..15794
                     /gene="ttdB"
                     /locus_tag="ACHUOY_000967"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR0681814.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15843..17306
                     /gene="ttdT"
                     /locus_tag="ACHUOY_000968"
     CDS             15843..17306
                     /gene="ttdT"
                     /locus_tag="ACHUOY_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR0681815.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17349..18362)
                     /gene="tsaD"
                     /locus_tag="ACHUOY_000969"
     CDS             complement(17349..18362)
                     /gene="tsaD"
                     /locus_tag="ACHUOY_000969"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR0681816.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18599..18814
                     /gene="rpsU"
                     /locus_tag="ACHUOY_000970"
     CDS             18599..18814
                     /gene="rpsU"
                     /locus_tag="ACHUOY_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR0681817.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18925..20670
                     /gene="dnaG"
                     /locus_tag="ACHUOY_000971"
     CDS             18925..20670
                     /gene="dnaG"
                     /locus_tag="ACHUOY_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0681818.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20865..22706
                     /gene="rpoD"
                     /locus_tag="ACHUOY_000972"
     CDS             20865..22706
                     /gene="rpoD"
                     /locus_tag="ACHUOY_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR0681819.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5503 a         6223 c         5834 g         5212 t
ORIGIN      
        1 ccaaagtaaa tattgcgtca ctaaatggac attggagtga catatgattt tattcgccga
       61 ctataatacc ccttatcttt tcgctatttc ctttgtactg ttgatcggcc ttttagaaat
      121 tttagcgctt atctgcggtc acatgctctc cggtgctctt gatgcacatc ttgaccatta
      181 taattccata accacaggtc atattagcca ggcacttcat tatctcaata tcggaagatt
      241 gccagctctc gtcgttctct gcttgctggc tggttttttt ggtcttattg gcatcctgtt
      301 gcagcatgcc tgcatcatgg tctggcaatc accactgtcg aacctgttcg tggttcctgt
      361 cagtttactg tttacgatta ttgcggtgca ttataccggt aaaatagttg cgccctggat
      421 tccacgcgac catagttcag ctattacaga agaagaatat attggtagca tggcattaat
      481 taccggtcat caagccactt caggtaatcc ctgcgaagga aaactcaccg atcaatttgg
      541 ccaaatacat tatttactac tggagcctga agaaggaaaa attttcacta aaggagtcaa
      601 agtgttaatt atttgccgac tttctgcaac gcgatatttg gcggaaaaca atccctggcc
      661 acaaatattg taatttatta atcaaaagga aatttaataa tggatgatat tgttaattct
      721 gtgccctcct ggatgtttac cgcgattatt gccgtatgca ttctgtttat tattggaatt
      781 attttcgcca ggctctatcg tcgcgcttcg gcagagcaag cttttgttcg tactggttta
      841 ggtgggcaaa aagtggtgat gagcggtggc gcaatcgtga tgccgatctt tcatgaaata
      901 atccccatca atatgaatac tctgaagctg gaagtcagcc gctcaaccat tgatagcctg
      961 attacgaaag atcgtatgcg tgtcgatgta gtagtcgctt tctttgtacg ggtaaaaccg
     1021 tctgtagaag gcatcgccac tgcggcgcag acgttggggc aacgcacgct atcaccagaa
     1081 gacttacgta tgttggttga agataaattt gtcgatgccc tccgtgcaac agctgcacaa
     1141 atgaccatgc atgagttaca ggatacccgc gagaacttcg tgcagggagt acaaaatact
     1201 gtcgcagaag acctatcgaa aaacggtctt gagctggaaa gcgtttcact taccaacttt
     1261 aaccagacct cgaaagaaca tttcaatccg aacaatgcct ttgacgccga aggtttaacc
     1321 aaactgactc aggagacgga gcgccgtcgt cgcgaacgta acgaagttga acaggatgta
     1381 gaagttgcgg tgcgtgagaa aaaccgtgat gcactttcgc gcaagttgga gattgaacaa
     1441 caagaagcgt ttatgacgct tgagcaggag cagcaggtta aaacccgtac tgccgaacag
     1501 aatgcacgta ttgcggcttt tgaagctgaa cgtcgtcgtg aagcagagca gacgcgaatt
     1561 ctggctgaac gacaaattca ggaaacggag atcgagcgcg agcaggccgt ccgctcaaga
     1621 aaggttgagg ctgaacgtga agttcgtatt aaagagatcg aacagcagca ggtcaccgaa
     1681 atcgccaacc agacgaaatc gatcgctatt gccgccaaat cggaacaaca gtcccaggca
     1741 gaagcgcgtg ctaaccttgc actcgcagaa gcagtaagtg cgcaacaaaa cgtagaaacc
     1801 actcgccaga ccgcagaagc cgatcgtgct aaacaagttg ccctaatcgc tgccgcgcag
     1861 gatgcagaaa ccaaagcggt tgaactgacc gtgcgggcga aagcagaaaa agaagccgca
     1921 gagatgcagg cggcggctat cgttgagtta gccgaagcta cacgtaaaaa gggtctggcg
     1981 gaagcagaag cacaacgtgc gctgaacgat gctatcaacg tactttctga tgaacaaacc
     2041 agccttaaat tcaaactggc cttgttgcag gcgctacctg cggtaataga gaaatccgtt
     2101 gagccgatga agtccattga cggtatcaag attattcagg tcgatggtct gaatcgtggc
     2161 agcgctgcgg gtgatgcaaa cacgggtaat gtggggggcg gaaacctagc ggaacaagca
     2221 ttatcagccg ctctctctta ccgcacacag gcaccgctga ttgactcctt gctcaatgaa
     2281 attggcgttt caggcggctc actggcggca ttgacttcac ccttaacctc aacaactccc
     2341 gtcgccgaaa acgtagaata aattttcagc tatgaacgtc cctggcacaa aaagccgggg
     2401 attttttata tctgcgttcc gctaaaaact gcaaatgatc aggccgttgc aacgttttgc
     2461 gtgaccgctc ggggaaggca aaactgcctc tgggaaagcg ttgcgcgagg tccggcgctc
     2521 atcaacaatc ggggagtcag caaagggctg aaacgggaaa gcccctcccg aggaaggggc
     2581 cttacagaag gaaagggtta tgatgaagct cgtcatcata ctgattgtgt tgttactcgt
     2641 aagtttcgca gcttattaac agccaatcag aggggggaga aatcctccct cacccttgtt
     2701 cctttacttt aggttgaaaa aaaatcagcg tcaagaaggt ttcattaaca catatccctg
     2761 gcccaaaaag ccggggattt tttatatctg cgttccgcta aaaggtgcaa atgctcagga
     2821 cgttgcagcg ttttgcgtga tcgctcgggg aaggcaaaac tgcttcaggg aaagcgttgc
     2881 gcggggtccg gcgctcatca acaatcgggg agtcagcaag gggctgaaac gggaaagccc
     2941 ctcccgagga aggggcctta tagaaggaaa gggttatgat gaagttcgtc atcatactgg
     3001 ttgtgttgtt actgttaagt ttcccgactt actaacaact gatcagaggg ggaagaaatc
     3061 ctcccttacc cttgttcctt tactttaggt tgaaaaaaca acagcgtcaa taggcctgcc
     3121 tgctacaaag cgagatctgt gaaccgcttt ccggttagcc ttttttatcc tgttggatct
     3181 tcttgatgat gttagtggtc gagcaaccgt cttcgaagtt gagcaccagc acttcgccgc
     3241 cgttggccca gacttcttta ctcccggcga tctcttctgg tttatagtcg ccgcccttca
     3301 ccagtaaatc tggcaggatc ccggcgatca agcgctgcgg cgtgtcttct tcaaacgaca
     3361 ccacccagtc gaccgcttcc agtgcgccca gcacaatcat acgctgttcg agcgggttaa
     3421 cggggcggga atcccctttc agccgtttgg tggaggcatc gctgttcaca gcgacaatca
     3481 gacggtcacc ctgtttgcga gcatttgcca gataggagac gtgcccggcg tgcaggatgt
     3541 caaagacacc gttggtcatt accacctttt cgccgcgttt acgcgctgcc gctacggcca
     3601 gcttcagttc ctcttcggtc atcacgccaa agccggtatc tgcgcgccca cgcactgcat
     3661 tttccagctc gatcggcgaa accgtggagg ttcccagttt gccgaccacc acgccagccg
     3721 ccgcattggc aaagaagcag gcttcttcca gcgaattacc cgctgccagc gttgccgcca
     3781 ggacgccaat caccgtgtcg cccgcaccgg taacgtcata cacttcctgc gcctgggttg
     3841 gcatatgcag cggcgcttta cccggttgca gcagcgacat accctgttcg gaacgggtca
     3901 ctaacagagc cgagagttcg taatcggcaa tcagtttcat gccgcgctca acaatctctt
     3961 cttcggtctt acatttaccg acaacagctt caaattccga gagattcggc gttaacagcg
     4021 tagcgccgcg gtagcgctca aaatcggtac cttttggatc aatcagcacc ggaacgcccg
     4081 ctttacgcgc cagttggatc atctgctgta cgcttgccag cgcacctttg gcgtagtcag
     4141 aaagcaccag cgcgccaatc gaactcagcg cctgattaat ccgctcgtgc agcggctgcg
     4201 gatcaacgcc ttcgaaacct tcttcgaaat ccagacggat cagctgttgg ttgcgggaaa
     4261 gtacccgtaa tttggtgatg gtcggatgcg tcggtacaga aacgaagtcg catttgacgt
     4321 tgacgtcggc cagagattta ctcagcgcgc gcgctgcatc gtcaatgccc gtcaacccga
     4381 ccaggcgtgc attagcaccg agagaagcga tattcatcgc cacgttagcc gcgccgcccg
     4441 gacgttcttc gatggtattc actttaacca cgggcaccgg cgcttccggc gagatacggc
     4501 tggagggacc gtaccagtaa cgatccagca ttacatcacc aaccaccatc actcctgcac
     4561 gttcaaactc tggcagcgtt actttcattc ctgtctcctg agagattcaa aatttgcgcg
     4621 cgataatacc atacttcatt cttccaccag ccacttctgc cagcttgccc gcaccagatc
     4681 acgctctgcg gtgaagcaat cccccgacac atggcccggc aattcctgta atgccagatg
     4741 atgaagttca tcgcgcagcg tcgtgtaagc acgggtcagc gccatcgctt cctgctcttc
     4801 cataatgtcg ttttgcgcca gcagctccag aatacgcacg ttgtctgacc agcgcgttaa
     4861 cttcggtttt tcatgagcgt agcgcaacac cagatattgg gtaataaatt cgatatcggt
     4921 aattccgcct tcatcagctt tgatatcaaa gcgatcgcga tgtttattgc cgagatgagc
     4981 gcgcattttc tcgcgcattt cccgcacttc cgtttgcaga gttctacctt cacgcggcag
     5041 cgtcataatc tcgcgacgca ctgcgtcaaa gtgcgcggtg agctgcggat cgccgtacac
     5101 tacccgcgca cgcaccagcg cctgatgttc ccacgtccag gcctcgtttt tctgataatc
     5161 ggcaaatgct tctgtggagg taaccagcat tcccgccgcc ccggacgggc gcagccgcgc
     5221 atccacttcg tacaaaatgc cggaagaggt tcgcgtgctg aacaggtgca taatgcgttg
     5281 cgccagacga agataaaact gtcgtccgtc gatttcccgc tcgccgtcag tcatcgcatc
     5341 cattgggcaa tcatggagga agatcaggtc aagatcggaa ctgtagccta actcccagcc
     5401 gcccagcttg ccgtagccga ccactgcaaa accacgccct tcgcgatcgt tgaggtgatt
     5461 cggcttaccg tagcgggcaa ccatttgaac ccacgcctgc tgaacgacgg catctatcat
     5521 ggcttccgcc agccaggtta agtgatcgct cactttcatc accggtagcg taccagcgat
     5581 atccgctgcg gcgatgcgta acagctgcgc ctgtttgaac tgacgcagcg cctcaagctg
     5641 ttgctcttcg tcatcttccg gcacgcgcag caaatactgg cgcaactcat cgcggtaggc
     5701 atcggtcgcc gtcggctggt aaagagtgtt tggatcgagc aattcatcca gcaataatgg
     5761 ataacgcgcc agctggctgg caatcatcgg cgatgcggca cacagagaaa tcaaatggtt
     5821 aagcgccgca gggaattcac tgagcaattc taaataggtg gtgcgggtaa caatccccac
     5881 cagcaaggcg gtaatgcgcg acagcgtgac agcagcgtct tcacgcgcac agacatcact
     5941 tagcagatgc ggcatcagat ggtcgagcac ctgacgtcct cgcggcccga tggtgcgctt
     6001 atccagctct ttgcggaaat cggaaatcag cgttagcacc tgtttgcgat catcctcgct
     6061 aagatgcgcc agcactggcg tagtgtcatc ttcctgcaac gcatcctgcc acagctcacg
     6121 ccactgttcc gacagcgact cttcctgagt ttcactttcg tcgtcgccaa tcaattcatt
     6181 aaacacccgg cgcacattgt tcatatgtgc ggtcagcgcc ccggtcagtt gcggccagtc
     6241 agcaaagtcc atcgcccacg ccagccgcgc acggttaagc tcatcagaag gaagcgtctg
     6301 ggtttgttcg tcgttaatgc tttgcagcag gttttccaga cgccgcagga acagatacgc
     6361 cactcgcaat tgttcagcat cgttttcaga aagcagatgc agcgcagcaa tggcgctgag
     6421 cgttggcagt aaagagcacg attgcagcga cggttcgcgc ccgccgcgaa tgagctggaa
     6481 tacctggacg ataaattcaa tttcgcgaat accacccgcg ccaagtttga tgttgtcggt
     6541 caaaccgcgc cgacgcactt cacgggcaat cattcctttc atgttgcgca gcgactgaat
     6601 cacgctgaaa tcgatgtaac gacggaaaac aaacgggcgt agcatcgcac gcaactcgtt
     6661 agcatagacg ccttcgctat cgcccataat ccgcgctttg accatcgcgt aacgctccca
     6721 gtcgcgcccc tgctcctggt aataatcctc caacgcggca aagctcagca ccagcgggcc
     6781 actttcgcca aacggacgca gtcgcatatc cacgcgatag acgaagccat cctgcgttgg
     6841 ttgatccagc actttaatca gccgctgccc catgcgggta aaaaactgcg cgttatccag
     6901 ttcccggcgt ccgccctgcg tacaaccgtg ttccggccag gcaaaaatca gatcgatatc
     6961 agaggagaaa ttcagttccc cgccgcccag cttacccatg cctaagatca gcagcggttg
     7021 cgcttcgccc tgcgcattgc acggcgttcc ccactcgcgg cagcaggcgt catacagcca
     7081 gtcacgcgcc gcaacaatca gcgtctccgc cagataactg agctgctgca atatgctctc
     7141 ttcagtaacc agtgccagcg tttgcgccca tgcgatgcgc accataatgc gccgccggaa
     7201 tagccgcagc tcgcgcatta acccggcttc gtcactcaca ttactgagcg cctcctgcaa
     7261 ccatgcagcg taatgctgcc attcatcggc ctgcggcggt tggctttcca gttccgtcaa
     7321 ccactctcga tgcgcaatta tgctgtcctg cacaaaatca ctgaaagtaa gtactgactt
     7381 cgcctgtgca cttagtggtt cctcggctaa aggctctggc agccgctcaa caacggtctg
     7441 cctgtactgc tgtaacggtg aagagagcgg cttcataagc gattttatcc ttgcctgatt
     7501 aacgttttcc gctgtgcaac cagaacggtt cctgattgtt tgcctcatta cggaaatgct
     7561 caatttcaat gcgttgcccg gtcgcaatag cgtgacgcag cccctgccag ttctccagcc
     7621 aggcatgcgc gacgacagga tcatagtaac ccgccagcaa cagtattgag tcaatgtcac
     7681 gcgtcaggcg tggcaactgg tcacggtaga gatcgcctaa cggctggcag aaaacgcttt
     7741 tcagttcagc ggcatggtgg gaaagatgga tatcggcaaa gcgtttgaag gagtcgctga
     7801 ttttgccctg cgctttggca tctaaaaatg gctgccatgc tttgcttacc aaccattcgg
     7861 tcaacgccag cttcgccatt gccgtttcgg tagagtagac cgccgtcacg gcagaaaccg
     7921 cagaagcaat ggtcgcttcg cattgggtca gcaaatcacg taagtgagtg ctcgctttac
     7981 gcggcacaat accaccgaac agcatcagcg tatgacggac caggctaatg gctgccagca
     8041 cctgttcttt cgccgcatcg ttaccgcgta cccacagttc ttcatgatac tgccattgcg
     8101 ctaacgccag ctcgagcgcc gcttccagcc cctgttctac atcggctttt gccgcaacat
     8161 gcaaaatggt ggtcggtttg atttcacgcg ccggattgcc ctgcgccaga tgatagccac
     8221 gcgccgcttt gctcaggctg ccctggcgta atccggtttg cgataccagt tggttcgcca
     8281 gtttcagcac cgcgcgcgtg tcgccgctaa gcagttccag ttccagctca cagataggtt
     8341 cagcaaattc ccccgctttc acctccccct gatcgagagc gatttcaatt cggctgccat
     8401 cgaccgccac cagccatttt tcgcgataaa aatcggtgct gaacagcggt tgtacgcggg
     8461 aagcgagatc ggcaggcaat tcaccgttcg gccagacttc cgagggaaac tgcgccaggt
     8521 cgagcgtcgg ttcgctcaac gccacgttat attccgggcg ctggtgtaag ccgcctgtca
     8581 ctcttcctgc aactttcatg gtcatctcat agcgaccgtt ttcgccacga atacgtaagc
     8641 ccatatcgtg cctacgcagc cagttatccg gcgtttcgta gtaaatattc agcaactgca
     8701 cggggtcatg gtgctcgccg cccagcgtat tgagatggtc acgcaacgcc tcaacggcac
     8761 tgtgattaac aataaacttt aattcgattt cctgagccat ggcctttact tatcggttat
     8821 gtcacataag tgacgatgaa cggcgaactt aatgcgatct tttttgtcag tagatagtat
     8881 tttgcgccaa attgccatgc aacgggcaat ttgacgggcg taaaagtttg aagcagtggc
     8941 aagacctgac acagaggatt ccgattgatg tcgaatgctt tgcgtagcga cactgatgcc
     9001 actactatcg ttccactttc catgacaata acgacagcct gatgccaaaa ttacgcctga
     9061 tcggattaac tttactcgca cttagcgcga ctgccgtctc acacgctgaa gaaacgcgct
     9121 atgtttctga cgaactgaat acctgggtcc gtagcggtcc gggagatcat tatcgcctcg
     9181 tgggcacggt taacgccggc gaggaagtga ccttattaca aactgacgcc aacaccaatt
     9241 atgcccaggt gaaagacagc tctggccgta ccgcctggat cccgttgaaa caacttagca
     9301 ctgagccaag cctgcgctcc cgtgtgccag atctggaaaa tcaggtcaaa accctgaccg
     9361 ataaactcac caatatcgat aacacctgga atcagcgcac cgcagaaatg cagcaaaaag
     9421 tggcgcagag cgacagcgtg atcaacgggt taaaagaaga aaatcagaaa ctgaaaaacg
     9481 agctgattgt cgcgcagaaa aaggtcgatg ccgccagcgt acagctggat gacaaacagc
     9541 gcaccatcat catgcaatgg tttatgtatg gcggtggcgt gctggggctt ggcttgctgc
     9601 tcggtctggt actgccgcac ctgatcccaa gccgcaaacg caaagatcgc tggatgaact
     9661 aaatcgcctt cttcgttgca ctgtcttatt attaggaatt gtgtatttga cgaagggatg
     9721 atggcgtgaa gatttatctg gtcggtggtg ctattcggga tgcattgtta gggctaccgg
     9781 tcaaagacag agattgggtg gtagtcggca gtacgccaca ggagatgctc gacgcgggct
     9841 accagcaggt aggccgcgat tttcctgtgt ttctacatcc gcaaacgcat gaagagtatg
     9901 cgctggcacg taccgaacgg aaatccggtt ccggttacac cgattttact tgctatgccg
     9961 caccggatgt cacgctggaa gatgatctta agcgtcgcga tctgaccatt aatgcgctgg
    10021 cccaggacga taacggcgag attatcgacc cgtacaacgg tctgggcgat ctgcaaaatc
    10081 gtctgttgcg ccatgtttcc cccgcttttg gcgaagatcc gttacgcgta ctgcgcgtgg
    10141 cgcgttttgc tgcgcgttat gcccacctcg gttttcgtat tgccgatgaa actctgacgc
    10201 tgatgcgcga gatgacccat gcgggtgaac tggaacacct gacgcctgaa cgggtatgga
    10261 aagagacgga aagcgccctc accacccgca atccacaggt gttcttccag gtactgcgcg
    10321 attgcggcgc actgcgcgtt ttattcccgg aaattgacgc actgtttggc gttccggccc
    10381 ctgccaggtg gcatccggaa atcgacacgg gtattcatac cttaatgacg ctctcaatgg
    10441 cggcgatgct gagtccgcag gtcgatgtcc gtttcgcaac tttatgccac gatctcggta
    10501 aaggactgac gccgccagaa ctttggccgc gtcatcatgg tcacggtccg gcgggtgtga
    10561 aattagtgga acaactctgc cagcgtctgc gcgtgccaaa tgaaattcgc gatttagcca
    10621 gactggtggc tgagtttcac gatctcatcc acaccttccc aatgctgaac ccaaaaacta
    10681 tcgtcaaatt gtttgattcc atcgacgcct ggcgtaaacc gcagcgtgtc gagcaactgg
    10741 cgctgaccag cgaggctgac gtacgcggca gaaccggttt tgaatcagcg gactacccgc
    10801 aaggccgttg gttgcgcgaa gcctgggaag tggcgcagtc agtgccgaca aaagcggtcg
    10861 ttgaagcggg atttaaaggt gtggagattc gcgaggagct gacccgacgg cggattgcgg
    10921 cggtagccag ttggaaggaa caacgttgcc caaagcctga ctgagtataa aactaaacgt
    10981 taacacgaac catcccctcg ccccttcggg gagagggtta gggtgagggg aacagaccag
    11041 caccggtgcg aacaatcacc cccaccccgg ccctctccct acagagcgag ggagaaaacc
    11101 aggcctgctc ccgaaccttt tctcaatcaa aagaacacga cgtacaccgc agccgccaca
    11161 ataaagcgat aaatggcgaa cgggataaac gaaatgcgct taatcaattg caggaaggtt
    11221 ttaatcgcta tcagcgccac cacaaaagcg gtgataaacc caacggcaaa catcgagata
    11281 tcgccggttg tcaggaagcc ccagcttttg tagagatcga gcgccgttgc gcccatcatc
    11341 atcggtaccg ccagcaggaa cgagaactcg gaagcagcgt aacggctcac gcccatcagc
    11401 atcccacctg aaatggtggc cccggaacgg gagaaacccg gccacagcgc cagacactgg
    11461 aaacagccaa tcataaacgc ctgacgatag gtcatatcat caagacccgg cgcacgcggc
    11521 tctttcggct tcaggcattc ggcggcaatc agcaacaaac cgccaacgac cagtgcatac
    11581 atcacattta tcgggttaaa caatgactta atcgtgtcgt ggaacaacag ccccagtacc
    11641 accgccggaa tcatccccag caaaatgtgg atcagcgtta aacgaccttt gctttctcct
    11701 tcgtgctgca acgggcggcc aaagtggatg ccaatcaggc caaacagacg ccgccagaac
    11761 atcactacta ccgccagaat tgatcctaac tggatcacaa cttcaaaggt tttcgccgtg
    11821 tcgccctcga accccaacaa gtgaccgaca ataatcatat ggcccgtgct ggataccggc
    11881 agaaattctg tcaatccttc gaccacaccc aatattgccg ctatcagcag cgagtgcata
    11941 tcgctcatca ataaacccct aaattattaa aatgtaccgc ttgtccggac tactgcagta
    12001 agaccaggtt ataaccgttt ggtttaacag ctgtaaaatt aattattttc tttcagatta
    12061 ttgccacgct caatgattac accaacattc gccgcccgcg ccattgcgcc tggcttgctg
    12121 agtttgatgc gcacccacgg cgagttgaag cgtgctaaca gcagctccgc cacctcttca
    12181 gccacgcgtt ccaccagcgc aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca
    12241 atgtcagcgt aactgaggca atccgccaca tcatcacttt tcgccgcttt acggttatcc
    12301 catgccattt cgatatcgaa cactaacttc tgttcgatgg tctgttccca gtcgtaaaca
    12361 ccaatagtgg tgattaccga aagttgctct ataaatacaa tatccatcac gtcctgcctg
    12421 ctttttggct aaccggatac cacttccggc gaaatgtgcg tattatccac agattcatcg
    12481 ttgaacacga attttcaaaa cggaacagct tatgagtgca atcgcgcctg gaatgatcct
    12541 catcgcgtac ctctgcggct ccatttccag tgccattctg gtttgccgct tgtgtgggct
    12601 gcccgatccg cgaaccagcg gctccggcaa tccaggcgca accaatgtgt tacgtatcgg
    12661 tggcaaggga gcagccgtag cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt
    12721 ctggggcgcg tatgaattag gtgtcagccc cttctggtta ggcttaattg ccatcgccgc
    12781 ctgtcttgga cacatctggc ccgttttctt cggatttaaa ggaggaaaag gcgttgctac
    12841 cgcttttggt gccatcgcac ccattggctg ggatctcacc ggagtaatgg cgggaacctg
    12901 gttactgacc gtgctattga gcggatactc gtcgctggga gcgattgtca gtgcactgat
    12961 tgctccgttt tatgtctggt ggtttaagcc acaattcacc ttcccggttt cgatgctctc
    13021 ttgcctgatc ctgctgcgtc atcatgacaa catccagcgt ctgtggcgtc gtcaggagac
    13081 aaaaatctgg acgaaattca aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga
    13141 tctacatgac ctgatagcct tcatcgggct tgcccagccg ttgctggcac catgccgcca
    13201 gaaattccac gcagacacgt aatttcatgc tgcgatagag cggctcccgg taaacagccc
    13261 agatattggc gctttgtgca tactccggca atacttgcac cagtttgcca ctctccagaa
    13321 acggcaacac atcccactcg gaacgcagca taatcccttt gccctccagc gcccattgca
    13381 acactatctc gccgctattg gaggaaagat gcccgctaac cttcaccgat tttttctcct
    13441 gaccgttccc caactcccat attccgtggg tcatatcgcg ctctttggtc accagacagt
    13501 catgacgact taattcctgt aatgattgtg gctgcggata tttttgcaga tactcaggtg
    13561 ctgcgcataa tattctttta tttttcgtta acagatgcgc aatataataa tcaggaattt
    13621 cgtcattaat acgaatatcc agatcaatat tatcctgcac taaatcaatt tgccgatcga
    13681 acaattcaaa atgcacctgt aactcaggat aattgcgcat aagttcggta atggctggcg
    13741 caatatggct gcgcccaaaa ccaaaactgc aaccaatacg aatcatcccc tccgggcgcg
    13801 ttttgattta cgtgacgtca tcgaccagtc gctgatattg ggtgaggatt tccagcgcat
    13861 gttcgtaaca acgttggcca ctttcagtca gcgccacgcc gcgagcggag cggttcagca
    13921 gcgttgttgc cagcgtattt tcaaggattt ggatccgctt ggtgacaaat gcaggcgtct
    13981 gcccaagcgt cgccgctgcg gcgctaaaac tcccgctgtg gacgatttct accagtacct
    14041 gcaagtcttt ggctaaaggc cagctattca gcatcgttaa ttatccgcag ttgtgataag
    14101 cgcagtgtat ttcgcaaaac attgccattc cctgctctgg cgcaatattc attcttattt
    14161 tcgcgttgat tcacgaattg ttaattacat attcacggtg gcaaaaaata taaaaccaca
    14221 tttttagtgg tagtttgtgg cggtgaattt ttccagacaa atacaaaaac tggagttgcc
    14281 atgatgagcg aaagtaataa gcaacaggca gtgaataagt tgacggagat tgtcgctaac
    14341 tttaccgcca tgatttctac ccgaatgcct gatgacgtgg tggataaact aaaacagcta
    14401 aaggatgccg aaacgtcgtc gatggggaaa attatctacc atacgatgtt cgacaacatg
    14461 caaaaagcga ttgacctgaa tcgtcctgcc tgtcaggaca ccggggagat tatgttcttc
    14521 gttaaagtcg gttcccgctt cccactgctt ggcgagctgc aaagcatact caaacaagcc
    14581 gtggaagagg caaccgtcaa agcgccacta cgtcacaatg cggtagaaat ttttgacgaa
    14641 gtaaacaccg gcaaaaatac cggtagcggc gtaccgtggg tcacctggga catcatcccc
    14701 gacaatgacg atgcggaaat cgaagtttac atggcaggcg gcggctgcac gctacctggc
    14761 cgctcgaaag tgttaatgcc gtcagaaggc tacgaaggcg tggtgaaatt cgtcttcgaa
    14821 aatatctcca ccctcgccgt aaacgcctgt ccaccggtac tggtgggcgt gggcatcgcc
    14881 acctcggtgg aaaccgccgc cgtactctcg cgtaaagcca ttttgcgccc gattggcagc
    14941 cgccatccca atccaaaagc ggcagaactg gagctacgcc tggaagaagg actcaaccgt
    15001 ctggggattg gtccacaagg gctgactggc aacagttcag tgatgggcgt acatatcgaa
    15061 tctgccgccc gccatccgtc aaccatcggc gttgctgtct ctaccggctg ctgggcgcat
    15121 cgtcgcggca cactgctggt tcatgccgat ctcacctttg aaaatctgtc tcacacccgg
    15181 agcgcgttat gaaaaagatc ctgacaaccc cgatcaaagc tgaagatctg caagatattc
    15241 gcgtcgacga tgtgatctac ctgaccggta cgctggtgac ctgccgtgac gtttgtcacc
    15301 gccgcttgat cgaactgaaa cgtccgatcc cttacgatct caacggcaaa gcgattttcc
    15361 acgctggccc catcgtgcgc aaaaacggcg ataaatggga gatggtttcc gttggcccga
    15421 caaccagtat gcgtatggaa agttttgaac gtgaatttat cgagcagacc ggcgtgaaac
    15481 tggtggttgg caaaggcgga atggggccgc tgaccgaaga aggctgccag aaattcaagg
    15541 cgctacatgt gattttcccg gcaggctgcg cggtgctggc ggcaacccag gtggaagaga
    15601 ttgaagaagt gcactggaca gagctcggaa tgccggagtc gctgtgggtc tgtcgggtca
    15661 aagagttcgg cccgctgatt gtctctattg atacccacgg caacaacctg atagccgaaa
    15721 acaaaaagct gttcgccgaa cgccgcgatc ccatcgtgga agagatctgc gagcacgtcc
    15781 attacatcaa ataaccctcc cggagaggct cgccccctct cctttttcgc aggcataaca
    15841 cgatgaaacc ttccactgaa tggtggcgat accttgcgcc gctggcggtc atcgccatta
    15901 ttgctctaat tccggttccc gcagggctgg agagtcatac ctggctctac tttgccgttt
    15961 ttactggcgt gatcgttgga ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg
    16021 tgggtatatc catcatcgcc atactctctc cctggctgct gttcagcccg gagcagctcg
    16081 ctcagccagg ctttaaattc actgcaaaat ccctctcgtg ggccgtttcc ggtttttcta
    16141 attcggttat ctggctgatt ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg
    16201 ggcttggacg ccgcattgcg ctgattctgg tgaaaaagat gggacatcgc acgctgtttc
    16261 tcggctatgc ggtgatgttc tccgagctga tcctcgcacc tgtaacaccg tccaactcgg
    16321 cgcgtggtgc ggggattatc tatcccatca tccgtaacct gccaccgctc tatcaatcac
    16381 aaccaaacga cagcagttcg cgcagcattg gctcgtacat catgtggatg gggattgttg
    16441 ccgactgcgt gaccagcgcc attttcttga cggcgatggc accaaacttg ctgttaattg
    16501 gactgatgaa aagcgcatct cacgccacgc tgagttgggg cgactggttc ctcgggatgt
    16561 tgccgctcag tattttgctg gtgctgctgg ttccctggct ggcttatgtg ctgtacccgc
    16621 cggtactgaa gtctggcgat caggtgccgc gctgggcaga gacggaactg caggcaatgg
    16681 gcccgctctg ttcgcgtgaa aaacggatgc tggggctgat agtaggcgcg ctggtgctgt
    16741 ggattttcgg cggtgattat atcgatgccg cgatggtcgg ttacagcgtg gtggcactga
    16801 tgctgcttct gcgcattatc agttgggacg acattgtcag taataaagcg gcgtggaacg
    16861 ttttcttctg gctggcctcg cttatcaccc tcgctaccgg actcaacaac accggtttta
    16921 ttagctggtt tggcaaactg ttagcaggca gcttaagcgg ttattcgcca acgatggtga
    16981 tggtggcgtt gattgtggtg ttttatctgc tgcgctactt tttcgccagc gccacggcgt
    17041 atacctccgc cctcgcaccg atgatgattg ccgctgcgct ggcgatgccg gaaatcccgc
    17101 tgccggtgtt ctgcctgatg gttggcgcgg caattggtct ggggagcatt cttacaccat
    17161 acgccaccgg acccagtccg atttactacg gtagtggtta tctgccaacg gcggattact
    17221 ggcgactggg ggcgattttt gggctgatat tcctcgtgtt gctggtgatt accggcttac
    17281 tgtggatgcc cgtggtgttg ctttaagtga ggacggtgcc ggaaattgct ctccggcacc
    17341 caatagtttt acgcagccgg taactccgcc agcggccagc gcggacgcac gctaacgccg
    17401 agatccgccg tcgcgcctgc tttaaaccgc accattccgg cataggcgat catcgcgccg
    17461 ttatcagtac aaaactccgg acgcgcgtag aacacttcgc cgcggcgttt tttcatcatt
    17521 tcagccagct tcgcccgtaa cgtgcggtta gcactcacgc cgcccgccat gaccagtcgc
    17581 ttaaagcccg tctgatccaa cgctcgctta cacttaatca tcaacgtatc gaccaccgca
    17641 tcttcaaagg cgcgggcgat atcagcacgc gtctggtcgt cggtgccgtt gtcacgaatc
    17701 gtgttcgccg caaaggtttt cagaccagaa aagctgaaat ccagccccgg acggtcggtc
    17761 atcggacgcg ggaaaacaaa gcgcccggca gtaccctgag ccgccatttt cgacagtaac
    17821 ggtccgccag gataatccag ccccagcagc ttcgcggttt tatcaaacgc ttcaccggcg
    17881 gcatcatcga tagactcgcc gagcagctcg tactgaccaa tgccagtcac gctgattaac
    17941 tgcgtatgac cgccggacac cagcagcgcg acaaacggaa attccggcgg gttatcttcc
    18001 agcatcggcg ctaacagatg cccttccata tggtgtaccg ggattgccgg aacgttccag
    18061 gcaaacgcca gagaacgccc cacggttgcg ccaaccagca gtgcgccgac taatccaggg
    18121 cctgcggtat aggccacagc atcaatgtct tttgccgtta agccagactc ctttagcgcc
    18181 gcctggatca acggtacggt tttgcgcacg tgatcgcggg aggccagttc aggcacgacg
    18241 ccgccatagt cagcgtgcaa tttcacctga ctatacaatt ggttggctaa caaacctttt
    18301 tcatcgtcgt aaatggcgat gccggtttca tcgcaggaag tttcaatacc cagtacacgc
    18361 atgacttgtt ttacctcgct ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa
    18421 aactttgttc gcccctggag aaagcctcgt gtatactcct cacccttata aaagtccctt
    18481 tcaaaaaggc cgcggtgctt tacaaagcag cagcaattgc agtaaaattc cgcaccattt
    18541 tgaaataagc tggcgttgat gccagcggca aaccgaatta atcaaaggtg agaggcacat
    18601 gccggtaatt aaagtacgtg aaaacgagcc gttcgacgta gctctgcgtc gcttcaagcg
    18661 ttcctgcgaa aaagcaggtg ttctggcgga agttcgtcgt cgtgagttct atgaaaaacc
    18721 gactaccgaa cgtaagcgcg ctaaagcttc tgcagtgaaa cgtcacgcga agaaactggc
    18781 tcgcgaaaac gcacgccgca ctcgtctgta ctaattcccc gagagcgttg ctctccgatc
    18841 agaccgagtt gtagttgtaa ggccgtgctt ccgaaaggaa tgcgcggctt attttcgttt
    18901 atgaattgct aaaaatcggg gcctatggct ggacgaatcc cacgcgtatt cattaatgat
    18961 ctgctggcac gcactgacat cgtcgatctg atcgatgccc gtgtgaagct gaaaaagcag
    19021 ggcaagaatt tccacgcgtg ttgtccattc cacaacgaga aaaccccgtc attcaccgtt
    19081 aacggtgaga aacagtttta ccactgcttt ggatgtggcg cgcacggcaa cgcgatcgac
    19141 ttcctgatga actacgacaa gctcgagttc gttgaaacgg tcgaagagct ggcagcaatg
    19201 cacaatcttg aagtgccatt tgaagcaggc agcggcccca gccagatcga gcgccatcag
    19261 aggcaaacgc tttatcagtt gatggacggt ctgaatacgt tttaccaaca atctttacaa
    19321 caacctgttg ccacgtctgc gcgccagtat ctggaaaaac gcggattaag ccacgaggtt
    19381 atcgctcgct ttgcgattgg ttttgcgccc cccggctggg acaacgtcct gaagcggttt
    19441 ggcggcaatc cagaaaatcg ccagtcattg attgatgcgg ggatgttggt cactaacgat
    19501 cagggacgca gttacgatcg tttccgcgag cgggtgatgt tccccattcg cgataaacgc
    19561 ggtcgggtga ttggttttgg cgggcgcgtg ctgggcaacg atacccccaa atacctgaac
    19621 tcgccggaaa cagacatttt ccataaaggc cgccagcttt acggtcttta tgaagcgcag
    19681 caggataacg ctgaacccaa tcgtctgctt gtggtcgaag gctatatgga cgtggtggcg
    19741 ctggcgcaat acggcattaa ttacgccgtt gcgtcgttag gtacgtcaac caccgccgat
    19801 cacatacaac tgttgttccg cgcgaccaac aatgtcattt gctgttatga cggcgaccgt
    19861 gcaggccgcg atgccgcatg gcgagcgctg gaaacggcgc tgccttacat gacagacggc
    19921 cgtcagctac gctttatgtt tttgccggat ggcgaagacc ctgacacgct ggtacgaaaa
    19981 gaaggtaaag aagcgtttga agcgcggatg gagcaggcga tgccgctttc cgcatttctg
    20041 tttaacagcc tgatgccgca agttgatctg agtacccctg acgggcgcgc acgtttgagt
    20101 acgctggcac tgccattgat atcgcaagtg ccgggcgaaa cgctgcgaat atatcttcgt
    20161 caggaattag gcaacaaatt aggcatactt gatgacagcc agcttgaacg attaatgcca
    20221 aaagcggcag agagcggcgt ttctcgccct gttccgcagc taaaacgcac gaccatgcgt
    20281 atacttatag ggttgctggt gcaaaatcca gaattagcga cgttggtccc gccgcttgag
    20341 aatctggatg aaaataagct ccctggactt ggcttattca gagaactggt caacacttgt
    20401 ctctcccagc caggtctgac caccgggcaa cttttagagc actatcgtgg tacaaataat
    20461 gctgccaccc ttgaaaaact gtcgatgtgg gacgatatag cagataagaa tattgctgag
    20521 caaaccttca ccgactcact caaccatatg tttgattcgc tgcttgaact gcgccaggaa
    20581 gagttaatcg ctcgtgagcg cacgcatggt ttaagcaacg aagaacgcct ggagctctgg
    20641 acattaaacc aggagctggc gaaaaagtga tttaacggct taagtgccga agagcgatcg
    20701 ggaagccccc gacagccgca ctgagaggca gcggcaaata tataagtacg ccctcgtaat
    20761 tatcgttggc ggtaaacaac cgttggattt cagcgttaac ggctgaagga catcgggtca
    20821 atcgcccaac accaacctca tgaaataagt gtggataccg tcttatggag caaaacccgc
    20881 agtcacagct gaaacttctt gtcacccgtg gtaaggagca aggctatctg acctatgccg
    20941 aggtcaatga ccatctgccg gaagatatcg tcgattcaga tcagatcgaa gacatcatcc
    21001 aaatgatcaa cgacatgggc attcaggtga tggaagaagc accggatgcc gatgatctga
    21061 tgctggctga aaacaccgcg gacgaagatg ctgccgaagc cgccgcgcag gtgctttcca
    21121 gcgtggaatc tgaaatcggg cgcacgactg acccggtacg catgtacatg cgtgaaatgg
    21181 gcaccgttga actgttgacc cgcgaaggcg aaattgacat cgctaagcgt attgaagacg
    21241 ggatcaacca ggttcaatgc tccgttgctg aatatccgga agcgatcacc tatctgctgg
    21301 aacagtacga tcgtgttgaa gcagaagaag cgcgtctgtc cgatctgatc accggctttg
    21361 ttgacccgaa cgcagaagaa gatctggcac ctaccgccac tcacgtcggt tctgagcttt
    21421 cccaggaaga tctggacgat gacgaagatg aagacgaaga agatggcgat gacgacagcg
    21481 ccgatgatga caacagcatc gacccggaac tggctcgcga aaaatttgcg gaactacgcg
    21541 ctcagtacgt tgtaacgcgt gacaccatca aagcgaaagg tcgcagtcac gctgccgctc
    21601 aggaagagat cctgaaactg tctgaagtat tcaaacagtt ccgcctggtg ccgaagcagt
    21661 ttgactacct ggtcaacagc atgcgcgtca tgatggaccg cgttcgtacg caagaacgtc
    21721 tgatcatgaa gctctgcgtt gagcagtgca aaatgccgaa gaaaaacttc attaccctgt
    21781 ttaccggcaa cgaaaccagc gatacctggt tcaacgcggc aattgcgatg aacaagccgt
    21841 ggtcggaaaa actgcacgat gtctctgaag aagtgcatcg cgccctgcag aaactgcagc
    21901 agattgaaga agaaaccggc ctgaccatcg agcaggttaa agatatcaac cgtcgtatgt
    21961 ccatcggtga agcgaaagcc cgccgtgcga agaaagagat ggttgaagcg aacttacgtc
    22021 tggttatttc tatcgccaag aaatacacca accgtggctt gcagttcctt gacctgattc
    22081 aggaaggcaa catcggtctg atgaaagcgg ttgataaatt cgaataccgc cgtggttaca
    22141 agttctccac ctacgcaacc tggtggatcc gtcaggcgat cacccgttct atcgcggatc
    22201 aggcgcgcac catccgtatt ccggtgcata tgattgagac tatcaacaaa ctcaaccgta
    22261 tttctcgcca gatgctgcaa gagatgggcc gtgaaccgac gccggaagaa ctggctgaac
    22321 gtatgctgat gccggaagac aagatccgca aagtgctgaa gatcgccaaa gagccaatct
    22381 ccatggaaac gccgatcggt gatgatgaag attcgcatct gggggatttc atcgaggata
    22441 ccaccctcga gctgccgctg gattctgcga ccaccgaaag cctgcgtgcg gcaacgcacg
    22501 acgtgctggc tggcctgacc gcgcgtgaag cgaaagttct gcgtatgcgt ttcggtatcg
    22561 atatgaacac cgaccacacg ctggaagaag tgggtaaaca gttcgacgtt acccgcgaac
    22621 gtatccgtca gatcgaagcg aaggcgctgc gcaaactgcg tcacccgagc cgttctgaag
    22681 tgttgcgtag cttcctggac gattaatcgg taggccggat caggcgttac gccgcacccg
    22741 gcactagtcc ctctgcacaa acgccacctt tt
//
LOCUS       DAZOYL010000034        22425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZOYL010000034 DAZOYL010000000
VERSION     DAZOYL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.034"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1676)
                     /locus_tag="ACHUOY_000973"
     CDS             complement(<1..1676)
                     /locus_tag="ACHUOY_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFR0681820.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVQGIIALQ"
     gene            complement(1750..2376)
                     /locus_tag="ACHUOY_000974"
     CDS             complement(1750..2376)
                     /locus_tag="ACHUOY_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR0681821.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            2517..2876
                     /locus_tag="ACHUOY_000975"
     CDS             2517..2876
                     /locus_tag="ACHUOY_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR0681822.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(2879..3148)
                     /locus_tag="ACHUOY_000976"
     CDS             complement(2879..3148)
                     /locus_tag="ACHUOY_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR0681823.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(3138..3734)
                     /gene="rsmD"
                     /locus_tag="ACHUOY_000977"
     CDS             complement(3138..3734)
                     /gene="rsmD"
                     /locus_tag="ACHUOY_000977"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR0681824.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            3884..5380
                     /gene="ftsY"
                     /locus_tag="ACHUOY_000978"
     CDS             3884..5380
                     /gene="ftsY"
                     /locus_tag="ACHUOY_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR0681825.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            5383..6051
                     /gene="ftsE"
                     /locus_tag="ACHUOY_000979"
     CDS             5383..6051
                     /gene="ftsE"
                     /locus_tag="ACHUOY_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR0681826.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            6044..7102
                     /gene="ftsX"
                     /locus_tag="ACHUOY_000980"
     CDS             6044..7102
                     /gene="ftsX"
                     /locus_tag="ACHUOY_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR0681827.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            7347..8201
                     /gene="rpoH"
                     /locus_tag="ACHUOY_000981"
     CDS             7347..8201
                     /gene="rpoH"
                     /locus_tag="ACHUOY_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR0681828.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            8472..9575
                     /gene="livJ"
                     /locus_tag="ACHUOY_000982"
     CDS             8472..9575
                     /gene="livJ"
                     /locus_tag="ACHUOY_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR0681829.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(9774..10157)
                     /gene="panM"
                     /locus_tag="ACHUOY_000983"
     CDS             complement(9774..10157)
                     /gene="panM"
                     /locus_tag="ACHUOY_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR0681830.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            10581..11690
                     /gene="livK"
                     /locus_tag="ACHUOY_000984"
     CDS             10581..11690
                     /gene="livK"
                     /locus_tag="ACHUOY_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR0681831.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            11738..12664
                     /gene="livH"
                     /locus_tag="ACHUOY_000985"
     CDS             11738..12664
                     /gene="livH"
                     /locus_tag="ACHUOY_000985"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR0681832.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            12661..13938
                     /gene="livM"
                     /locus_tag="ACHUOY_000986"
     CDS             12661..13938
                     /gene="livM"
                     /locus_tag="ACHUOY_000986"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR0681833.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            13935..14702
                     /gene="livG"
                     /locus_tag="ACHUOY_000987"
     CDS             13935..14702
                     /gene="livG"
                     /locus_tag="ACHUOY_000987"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR0681834.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            14704..15417
                     /gene="livF"
                     /locus_tag="ACHUOY_000988"
     CDS             14704..15417
                     /gene="livF"
                     /locus_tag="ACHUOY_000988"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR0681835.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            15701..16765
                     /gene="ugpB"
                     /locus_tag="ACHUOY_000989"
                     /pseudo
     CDS             15701..16765
                     /gene="ugpB"
                     /locus_tag="ACHUOY_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            16863..17750
                     /gene="ugpA"
                     /locus_tag="ACHUOY_000990"
     CDS             16863..17750
                     /gene="ugpA"
                     /locus_tag="ACHUOY_000990"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR0681836.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            17747..18592
                     /gene="ugpE"
                     /locus_tag="ACHUOY_000991"
     CDS             17747..18592
                     /gene="ugpE"
                     /locus_tag="ACHUOY_000991"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR0681837.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSKK"
     gene            18594..19664
                     /gene="ugpC"
                     /locus_tag="ACHUOY_000992"
     CDS             18594..19664
                     /gene="ugpC"
                     /locus_tag="ACHUOY_000992"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR0681838.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            19661..20404
                     /gene="ugpQ"
                     /locus_tag="ACHUOY_000993"
     CDS             19661..20404
                     /gene="ugpQ"
                     /locus_tag="ACHUOY_000993"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0681839.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20391..20831)
                     /locus_tag="ACHUOY_000994"
     CDS             complement(20391..20831)
                     /locus_tag="ACHUOY_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR0681840.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            20951..>22425
                     /gene="ggt"
                     /locus_tag="ACHUOY_000995"
     CDS             20951..>22425
                     /gene="ggt"
                     /locus_tag="ACHUOY_000995"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR0681841.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVL"
BASE COUNT         5220 a         5750 c         6312 g         5143 t
ORIGIN      
        1 tcctgcaatg caatgatacc ctgcacgtca tcattacgta ctaccagcac aaccgtttgc
       61 ccggcgcttt ccagttcatt aatcaaacca gcaaatgcat cagcgggatg tttcccggca
      121 gcgcaaatca atacgcgctc accgttaacc tgcgcttcaa tgccagaccc gaccagcgcc
      181 cgctgtgatt cggcggtggg aatggcgagt tcagcaacct gtgcttcgcg tacgatggct
      241 tgcgccagtg gatgcgtcgc gccttgctcg accgccgccg ccagtgtcag cagttcagat
      301 tcactaatac ccgttgccgg atgaatcgcg gtaacgcgcg gtttaccgac ggtcagcgta
      361 ccggttttat caaacgccac ctgagtaaca cgacctagtt gttccagcgc cgctccgcct
      421 ttaatcaacg ccccacgacg cgctgccgcc gccagcccgg aggtaatcgc cgcaggcgtt
      481 gagataacta acgcacacgg gcagccaatc agcagcagcg tcagcccttt ataaatccac
      541 tcctgccagc tggcggcaaa cagcagcggt ggcaccagcg tcaccagcag agcgacggcc
      601 ataatcgcgg gcgtatagat acggctgaaa cggtcgataa accgctcaat gggggcgcga
      661 cgctcttcgg cttcttcgat cagtttcaga atccggtcaa tggcgctggc acccggttct
      721 gacagcactt ccagcgttac cagacggtct acgctggtgg ctcctgcagg aactttatcg
      781 cccgtcgcgc gctccaccgg aatggattct ccggtcaggg cgctttcatc aaaactggca
      841 aacggtgaga gcagtttacc gtcggcaggc aaacgcccac ctgcggcgac ttcaatcaca
      901 tcgccaggcc gcaggctgtt aatcgccacc tcttcccgct caccgttacg caggcgcgtg
      961 gcggtttctg gtttcagcgc cattaacgcg ctaacgccct gacgcgcgcg gctggcggcc
     1021 cagccttcca gtcgttcacc aatcaaaaac agcagcaaca ccatcgcagc ttcagccgtt
     1081 gcgccaataa acagtgcacc aatagcggct acgctcatta aggtttcaat ggcgaagtag
     1141 ctgccggatt tgatcagccg taatgcctga cgagcaatcg ggtacagccc aaccagcgtg
     1201 gtcgcgataa acgccagttg cccgaacgga tgattgaact gctccagacc ccagctgatt
     1261 gccatcatca cgattagtgt aatcagcggc agattctctt tcaggcgcga tgcttgtggc
     1321 tcgtcggcgg cctgctcatc gcgcagggaa tagcccgctt tttgcaccgc agattcaact
     1381 tgtgcacgaa tgtcattgtc ggcatcaacc accagttttt cggtggcgaa caacacctgc
     1441 acctgattca cacctgcaag ctggcgcacg gcattttcta ccttgcgcgc acaggcggcg
     1501 cagtccatgc cactgacttt ccagctatag cgggtgccgg agacgttttc agagagcgtt
     1561 ggcgagctgg agcatgcgcc gtcgcagcaa cagtcgttgg cgttctgtac cgtggttagc
     1621 ggtttgaacg cagcaaattg aggggctttc ttgccgtgat tgtcaggtgt cgacatggca
     1681 tactccggtt aagttttttc tcattaaccg aaggatacac tctggagtcg actccagagt
     1741 caagttttat cagagataca gtgagcggat gatgaggaag tgaccggcaa agtagcaggc
     1801 cgcagcaatc gcgttatccg cacggaagcg tcggcgatag tggctcccca gccagacaaa
     1861 gttactgata aacagcaacg aagcgccaac aaacgcagag agcgccggag cggtcggacg
     1921 gaagaaccac agttcacctg ccagccacac catcaccagc gtcatgccga taaacgtgca
     1981 gataggccaa cgatactctt ccagccgcgt ccagataatc gccagtaaca gcgcacccag
     2041 aaccagcaac accagtggca gaggccagaa gaaagagagc gtcatctgac tggcgaaata
     2101 gatggtgtac agcaggtgcg agaggaaaaa cgcgccgatg gcgtacatca gacgttgacg
     2161 tggcaacagg gttagcgcat cgcccagcag tgaggcgcac agccctgcca gcaccagata
     2221 gctaatggcg tcgaacatcg gcgcttgcca ggccagtaac agcaggagaa gaagggttaa
     2281 cggtttaaac acccagcgtt gccaggttgg cccacgatac gatgcatcca cagatagcca
     2341 tgcggaaaga cagacagcga taaacgacca aagcatctta gttccttgaa ttcgttattt
     2401 tttcgtaagc ggaatgccta cggtcttgct tccagtttag tggcgagggt ggacggatga
     2461 caacaccata actggcaggg tatgcttatt tccgcatttt ccgatgaggg ataaagatga
     2521 gcaaaccacc tcttttcttt attgttatca ttggcttaat tgtcgtcgcc gcatcgtttc
     2581 gttttatgca acagcgacgg gaaaaagctg ataatgatat ggctccgctc cagcaaaagc
     2641 tggtggtggt gagcaacaag cgggaaaaac cgattaacga tcgccgttcg cgccagcagg
     2701 aagtgactcc ggcaggcacc agtatgcgct acgaggcaag cttcaaaccg caaagcggag
     2761 gaatggagca gacgtttcgc ctcgacgccc agcagtacca cgccctgaca gtgggcgata
     2821 aaggtacgct gagctataaa ggaacgcgct ttgttagctt tgttggcgaa caataaggtt
     2881 attttttaac tttaaatttc ttctgccaga ccagcagttc aaacacgccg aaaaggaaaa
     2941 tccgtacctt ttcggcggtg gtcatctgcg ggccatcttt cggtaaagtg gatttcaata
     3001 gcgccagctg cataccatgc atcagcacgg taaaaatcag cgccacgtta acgaaaatat
     3061 ttagcgggcg cgggaaggga tgcaccagat tgaggattaa aaatccccaa acgcagagca
     3121 ttaacaaacg accaatatta atcagcatca ctttctcctt gtgcttcgcg ttgatacagc
     3181 cgataagcca cctgacccgc cactttttcc cgatgtaacg accagtttgc cggaacggtg
     3241 ggcagaccgt tttcgacttc gctttcgaca taaatcaggg cttcgtcagc cagccagccg
     3301 ttatcttcca gtaaatttat cgtctcttct aacaagccac ggcggaacgg tggatcgaca
     3361 aacacgatat tatgcggtgt gcctttttgc gccaggaatg acatcgcgtt gctgttcacc
     3421 acgcgtgcat tgcctgcttt tagtgtcgcc agattcttaa ttaactgctg agaaactgca
     3481 cgatccatct caattaacgt ggcccccgca gcgtagcgcg ataacgcttc cagccccagc
     3541 gcgccgctcc cggcgaagca atccagacat tgggcgtcaa caatgaccgg agccagccag
     3601 ttaaacaacg tttcgcgtac gcggtcggtg gtggggcgca gacctgggct atcaggaacc
     3661 gggagtttac ggcctcgcca ctgcccgcca ataatgcgga tttggccgct gccggaatga
     3721 ttcggttttt tcatgataaa ttgctcaatc cgccagataa cacatacttg caggcggtga
     3781 tgtgaattaa gttaagtgat agactatttc atcatttttt tagctgctat gtacatagcg
     3841 ttaacgctgt gccatgaagc aacagcgagg agtgtagtcg caaatggcga aagaaaaaaa
     3901 acgtggcttt ttttcctggc tgggctttgg tcaaaaagag cagaccccgg aaaaagagac
     3961 agaagttcag aatgaacaac cggttgtaga agaaatcgtt caggcgcaag agcctgtgaa
     4021 ggcttcggaa caagccgttg aagagcagcc gcaggcgcat actgaagccg aggcggaaac
     4081 ttttgctgcc gacgttgtgg aagtcactga acaggttgtt gaaagtgaaa aagcgcagcc
     4141 tgaagcggaa gttgttgcac agccggaacc ggtcgtagaa gaaacgccgg agccagtggc
     4201 tatcgaacgt gaagagctgc cgttgccgga agacgtcaac gccgaagagg tttcgccaga
     4261 agagtggcag gctgaagcgg aaaccgtaga gattgtcaaa gcggcggaag aagaagcggc
     4321 taaagaagaa atcaccgacg aagagctgga agcacaggcg ctggcagccg aagcagcaga
     4381 agaggcggtg atggtggttc ctccggcaga agaagagcag ccggtggaag aaatcgctca
     4441 ggagcaggaa aaaccaacca aagaaggttt cttcgcgcgc ctgaaacgca gcctgttaaa
     4501 aaccaaagaa aatctcggtt ccggatttat cagcctgttc cgcggtaaaa aaatcgacga
     4561 tgatctgttt gaggagctgg aagagcagct tttgatcgcc gatgtgggcg tggaaaccac
     4621 acgtaaaatt atcaccaatc tgacggaagg cgcatcccgc aagcagcttc gtgatgccga
     4681 ggcgctctat ggcctgctga aagaagagat gggtgagatt ctggcgaaag tcgatgagcc
     4741 gctgaatgtt gaaggtaaaa cgccgttcgt gatcctgatg gtgggcgtca acggtgtggg
     4801 taaaaccacg acgattggta agctggcacg tcagtttgag cagcagggta aatcggtgat
     4861 gctggcggcg ggcgatactt tccgtgcagc agcggttgaa cagcttcagg tctggggtca
     4921 gcgcaacaat attccggtga ttgcccagca taccggggcg gattccgcct ctgttatctt
     4981 cgacgccatt caggcagcta aagcgcgtaa tatcgacgtc ctgattgccg ataccgccgg
     5041 acgcctgcag aacaaatcgc acctgatgga agagttgaag aaaatcgtcc gcgtgatgaa
     5101 gaaactcgac gttgaagcgc cgcatgaagt tatgctgact attgatgcca gcaccgggca
     5161 gaacgcggta agccaggcca aactgttcca cgaagctgtt ggcttaaccg gcatcacgct
     5221 aacgaaactg gacggcacgg cgaaaggcgg ggtaattttc tcggtggctg accagtttgg
     5281 tatccctatc cgctacattg gtgtcggcga acgtattgag gatttgcgtc cgtttaaggc
     5341 ggacgacttt atagaggcac tttttgcccg agaggattaa caatgattcg ctttgaacat
     5401 gtcagcaagg cttatctcgg tgggagacag gcgctgcagg gcgttacgtt ccatatgcag
     5461 ccgggtgaga tggcgtttct gaccggtcat tccggcgcag ggaaaagtac cctcctgaag
     5521 ctgatctgtg ggattgagcg gcccagcgcc gggaaaatct ggtttagcgg ccatgacatc
     5581 acgcgtctga aaaaccgtga agttccgttt ctgcgccgcc agattggcat gattttccag
     5641 gatcaccatc tactgatgga ccgtactgtc tacgataacg tggcgatccc gctgattatc
     5701 gctggtgcca gcggtgacga tattcgtcgc cgggtgtcgg cggcactgga taaagtcggg
     5761 ctactggaca aagcgaagaa cttccctatt cagctttccg gcggtgaaca acagcgtgtt
     5821 ggcattgccc gcgcggtggt gaataagccc gcggtactgc tggcggacga accgaccggt
     5881 aacctggacg acgcgttgtc ggaagggatt ctacgtctgt ttgaagagtt taaccgcgtt
     5941 ggggtaaccg tattgatggc aacgcacgac atcaacctga tttcacggcg ttcccatcgc
     6001 atgctcaccc tgagcgatgg tcacttgcat ggaggcgtgg gccatgaata agcgcgatgc
     6061 aatcaatcat attcggcagt ttggtgggcg tctggatcgc ttccgtaaat cggtcggcgg
     6121 ctcaggcgac ggcggtcgta acgcaccgaa acgcgcgaaa tcctcgccaa aaccggtaaa
     6181 tcgcaaaacc aacgttttca acgaacaggt gcgctatgcc ttccacggcg cattgcagga
     6241 tctgaaaagc aaaccgttcg ccacgttttt aacggtgatg gttatcgcca tctccctgac
     6301 gctgcccagc gtctgttata tggtgtacaa aaacgttaat caggcggcga cgcagtatta
     6361 tccgtcaccg caaatcactg tttacttgca aaaaacgctg gacgatgacg ccgctgcggg
     6421 cgtggtggca cagttgcagg ccgagcaagg cgtggagaaa gtgaactatc tttctcgtga
     6481 agacgcactg ggcgagttcc gtaactggtc tggttttggt ggtgcgctgg atatgctgga
     6541 agaaaacccg cttccggcag tggcggtggt gatcccgaaa ctcgattttc aggggacgga
     6601 atcactcaat acgctgcgtg atcgtatcac gcagattaac ggcattgacg aagtgcggat
     6661 ggatgacagc tggtttgccc gtctggcggc gttgaccggg ctggtcgggc gcgtttcggc
     6721 gatgatcggc gtgttgatgg tggcggcagt gttcctcgtc atcggcaaca gcgtacgtct
     6781 gagcatcttt gctcgccgtg actccatcaa cgtgcagaaa ctgattggtg cgacggatgg
     6841 tttcatcctt cgcccgttcc tgtatggtgg cgcactgctg ggattttctg gcgcattgtt
     6901 gtcattaatt ttgtcagaaa ttctggtgct gcgattgtca tcggcggttg cggaagtggc
     6961 acaggttttc ggaacgaagt ttgatatcaa tgatttatcg ttcgatgaat gcctgctatt
     7021 gctgctggta tgctcgatga ttggctgggt ggcagcgtgg cttgccacgg tacaacattt
     7081 acgccacttt acgcctgaat aataaaagcg tgttatactc tttccctgca atgggttccg
     7141 tagcagggaa agagatcccg ttgtctcttc ccggtatttc atctctatgt cacattttgt
     7201 gcgtaattta ttcacaagct tgcattgaac ttgtggataa aatcactgtc tgataaaaca
     7261 gtggatgata acctcgttgc tcttaagctc tggcacagtt gttgctacca ctgaagcgcc
     7321 agaagatatc gattgagagg atttgaatga ctgacaaaat gcaaagttta gctttagccc
     7381 cagttggcaa cctggattcc tacatccggg cagctaacgc gtggccgatg ttgtcggctg
     7441 acgaggagcg ggcgctggct gaaaagctgc attaccatgg cgatctggaa gcagctaaaa
     7501 cgctgatcct gtctcacctg cggtttgttg ttcatattgc tcgtaattat gcgggctatg
     7561 gcctgccaca ggcggatttg attcaggaag gtaacatcgg cctgatgaaa gcagtgcgcc
     7621 gtttcaaccc ggaagtgggt gtgcgcctgg tctccttcgc cgttcactgg atcaaagcag
     7681 agatccacga atacgttctg cgtaactggc gtatcgtcaa agttgcgacc accaaagcgc
     7741 agcgcaaact gttcttcaac ctgcgtaaaa ccaagcagcg tctgggctgg tttaaccagg
     7801 atgaagtcga aatggtggcc cgtgaactgg gcgtaaccag caaagacgtt cgtgagatgg
     7861 aatcacgtat ggcggcacag gacatgacct ttgacctgtc ttccgacgac gattccgaca
     7921 gccagccgat ggcaccggtg ctctatctgc aggataaatc atctaacttt gccgacggca
     7981 tcgaagatga taactgggaa gagcaggcgg caaaccgtct gaccgacgcg atgcaaggtc
     8041 tggacgaacg cagccaggac atcatccgcg cgcgctggct ggacgaagac aacaagtcca
     8101 cgttgcagga actggctgac cgttacggtg tttccgctga acgtgtacgc cagctggaaa
     8161 agaacgcgat gaaaaaactg cgcgctgcta tagaagcgta atttccgcta ttaagcagag
     8221 aaccctggat gcgagtccgg ggtttttgtt ttttgggcct ctacaataat caatttcccc
     8281 tccggcaaaa cgccaatccc cacgcagatt gttaataaac tgtcaaaata gctattccaa
     8341 tatcataaaa atcgggtatg ttttagcaga gtatgctgct aaagcacggg tagccatgca
     8401 taaaacgaaa taaagtgctg aacaacaaca tcacaacaca cgtaataacc agaagaatgg
     8461 ggattctcag gatgaacata aagggtaaag cgttactggc aggatgtatc gcgctggcat
     8521 tcagcaatat ggctctggca gaagatatta aagtcgcggt cgtgggcgca atgtccggtc
     8581 cggttgcgca gtacggtgac caagagttta ccggcgcaga gcaggcggtt gcggatatca
     8641 acgctaaagg cggcattaaa ggcaacaaac tgcaaatcgt aaaatatgac gatgcctgtg
     8701 acccgaaaca ggcggttgcg gtggcgaaca aagtcgttaa cgacggcatt aaatatgtga
     8761 ttggtcacct ctgttcttca tcaacgcagc ctgcgtctga catctacgaa gacgaaggca
     8821 ttttaatgat caccccagcg gcaaccgcgc cggagctgac cgcccgtggc tatcagctga
     8881 tcctgcgcac caccggcctg gactccgacc aggggccgac ggcggcgaaa tatattcttg
     8941 agaaagtgaa accgcagcgt attgctatcg ttcacgacaa acagcaatac ggcgaaggtc
     9001 tggcacgagc ggtgcaggac ggcctgaaga aaggcaatgc taacgtggtg ttctttgatg
     9061 gtatcaccgc cggggaaaaa gatttctcta cgctggtggc gcgtctgaag aaagagaata
     9121 tcgacttcgt ttactacggc ggttaccacc cggaaatggg gcaaatcctg cgtcaggcac
     9181 gcgcggcagg gctgaaaact cagtttatgg ggccggaagg tgtggctaac gtttcgctgt
     9241 ctaacattgc gggcgaatca gcggaagggc tgctggtgac caagccgaag aactacgatc
     9301 aggttccggc aaacaaaccc attgttgacg cgatcaaagc gaaaaaacag gacccaagcg
     9361 gcgcattcgt ttggaccacc tacgccgcgc tgcaatcttt gcaggcgggc ctgaatcagt
     9421 ctgacgatcc ggctgaaatc gccaaatacc tgaaagcgaa ctccgtggat accgtaatgg
     9481 gaccgctgac gtgggatgag aaaggcgatc tgaaaggctt tgagttcggc gtatttgact
     9541 ggcacgccaa cggcacggcg acagatgcga agtaatcatt aatcggcaac tttgggttgc
     9601 cgccaaattg ctaatatcga gtacgttgct tcatgccgga tgcggcgtaa acgccttatc
     9661 cggcctataa gatccaaaga aatcagtaaa ttgcaacaca cattgtaggc ctgataagcg
     9721 tagcgcatca ggcaatttct cgttggtcat cacttttgaa atcaacttga cgattaacgc
     9781 ttctcccagc cgccctgttg cgccgtaaat cccagtgcct gcataaacgc tgtcatcaca
     9841 ccgcgatctt ccacgcctgc atccgccatc caccagcatg aaacgccagg attgttacgc
     9901 aaaacctctt ccagcagata ttgccccaca ccgcgacgtc gggtgacttc ccgcacacgc
     9961 agggaatcca gtgctccctc ggtgccgctt aaggttaccc gtacggcagc gagcaggcgc
    10021 tcgttaaaac gcgcggcgta gatacggtgg ttatcgtcaa cctgtaaaga ggaaggggaa
    10081 tactccggcc agatcttttg caggtcaatc cggtcctggt cgctaaagtt ttctaatcga
    10141 atgatggtca gcttcatggg taacccgtgt aaatcacaaa agtggaacca gtgtagcgaa
    10201 ataatttaat cggaggcttt ctctttttta tttcttttgg caggtgatta attttttaac
    10261 agcaataatt acaaagttaa aacattagag aatgaaaaat acccagcata atcccctgaa
    10321 tggtagtgaa ttattccgcc ctttgtgccg ttattttatg ctgacaaagg cacttttttc
    10381 tgtttatcta tcaataaatt cagaatatta tctgttctta atcgactgaa aaatagggat
    10441 tttaatcact gttatcacaa aatactgcgc taaaccctta atcagacagg caaaaacagt
    10501 gcagtataaa aaaagaacag tctgatttgt taacacataa aaacaaagca acacaacatc
    10561 acgaatgggg atttttgact atgaaacgga atgcgaaaac tatcatcgca gggatgattg
    10621 cactggcaat ttcacacacc gctatggctg acgatattaa agtcgccgtt gtcggcgcga
    10681 tgtccggccc gattgcccag tggggcgata tggaatttaa cggcgcgcgt caggcgatta
    10741 aagacattaa tgccaaaggg ggaattaagg gcgacaagct ggttggcgtg gaatatgacg
    10801 acgcctgcga cccgaaacaa gccgttgcgg tcgccaacaa aatcgttaat gacggcatta
    10861 aatacgttat tggtcatctg tgttcttctt ctacccaacc tgcatcagat atctacgaag
    10921 acgaaggtat tctgatgatc tcgccgggag cgaccaaccc ggagctgacc caacgcggtt
    10981 atcaacacat tatgcgtact gccgggctgg actcttccca ggggccaacg gcggcaaaat
    11041 acattcttga gacggtgaag ccccagcgca tcgccatcat tcacgacaaa caacagtatg
    11101 gcgaagggct ggcgcgttcg gtgcaggacg ggctgaaagc ggctaacgcc aacgtcgtct
    11161 tcttcgacgg tattaccgcc ggggagaaag atttctccgc gctgatcgcc cgcctgaaaa
    11221 aagaaaacat cgacttcgtt tactacggcg gttactaccc ggaaatgggg cagatgctgc
    11281 gccaggcccg ttccgttggc ctgaaaactc agtttatggg gccggaaggt gtgggtaacg
    11341 catcattgtc gaatattgct ggcgatgctg ccgaaggcat gttggtcact atgccaaaac
    11401 gctatgacca ggatccggca aaccagggca tcgttgatgc gctgaaagca gacaagaaag
    11461 atccgtccgg gccttatgtc tggatcacct acgcggcggt gcaatctctg gcgactgccc
    11521 ttgagcgtac tggcagcgat gagccgctgg cgctggtgaa agatttaaaa gctaacggtg
    11581 caaacaccgt gattgggccg ctgaactggg atgaaaaagg cgatcttaag ggatttgatt
    11641 ttggtgtctt ccagtggcac gccgatggtt catccacggc agccaagtga tcatcccacc
    11701 gcccgtgaaa tgcgggcggg tttagaaagg ttaccttatg tctgagcagt ttttatattt
    11761 cttgcagcag atgtttaacg gcgtcacgct gggcagtacc tacgcgctga tagccatcgg
    11821 ctacaccatg gtttacggca ttatcggcat gatcaacttc gcccacggcg aggtttacat
    11881 gattggtagc tatgtctcct ttatgatcat cgccgcactg atgatgatgg gcattgatac
    11941 cggctggctg ctggtggcgg cgggattcgt cggcgcaatc gtcattgcca gcgcctacgg
    12001 ctggagtatc gaacgggtag cctatcgccc ggtgcgtaac tctaagcgcc tgattgcgct
    12061 catctcggca atcggcatgt ccatcttcct gcaaaactac gttagcctga ccgaaggttc
    12121 gcgtgacgtg gcgctgccca gtctgtttaa cggtcagtgg gtggtggggc atagcgaaaa
    12181 cttctctgcc tctattacca ccatgcaggc agtgatctgg attgttactt tcctcgccat
    12241 gctggcgctg acgattttca ttcgttattc ccgtatgggg cgcgcctgcc gtgcctgcgc
    12301 ggaagatctg aaaatggcga gcctgcttgg catcaacacc gaccgggtga ttgctttgac
    12361 ctttgttatt ggcgcggcga tggcggcagt ggctggcgta ctgctcggtc agttctacgg
    12421 cgtcattaac ccctatatcg gctttatggc cgggatgaaa gcctttaccg ctgcggtgct
    12481 cggtgggatt ggcagcattc caggggcgat gattggcggg ctgatcctgg ggattgcgga
    12541 ggcgctctct tctgcctatc tgagtacgga atataaagat gtggtctcat tcgccctgct
    12601 gattctggtg ctgctggtga tgccgaccgg tattctgggt cgcccggagg tagagaaagt
    12661 atgaaaccga tgcatattgc aatggcactg ctctctgccg cgatgttctt tgtcctcgct
    12721 ggcgtcttta tgggcgtgca actggagctg gatggcacca aactggtggt cgacagcgct
    12781 tcggatgtcc gttggcagtg ggtgtttatc ggcacggcgg tggtcttttt cttccagctt
    12841 ttgcgacctg ctttccagaa agggctaaaa agcgtttccg ggccgaagtt tattctgccc
    12901 gcgattgatg gctccacggt gaagcagaaa ctgttcctcg tggcgctgct ggtgcttgcg
    12961 gtgtcgtggc cgtttatggt gtcacgtggg acggtggata tcgccactct gaccatgatc
    13021 tacattatcc tcggtctcgg gctgaatgtg gttgttggtc tttctggtct gctggtgctg
    13081 gggtacggcg gtttttacgc catcggcgct tacacttttg cgctgctcaa tcactattac
    13141 ggtctggggt tctggacctg tttgccgatt gccggactga tggcagcggc ggcgggcttc
    13201 ctgcttggtt ttccggtgtt gcgtctgcgc ggtgattatc tggcgatcgt taccctcggt
    13261 ttcggcgaaa ttgtgcgcat attgctgctc aataacaccg aaattaccgg cggcccgaac
    13321 ggtatcagtc agatcccgaa accgactttc ttcggccttg agttcagccg taccgcccgt
    13381 gaaggcggtt gggacacgtt cagtaatttc tttggcctga aatacgatcc ctccgatcgc
    13441 gtcatcttcc tgtatctggt ggcgttgctg ctggtggtgc taagcctgtt tgtcattaac
    13501 cgcctgctgc ggatgccgct ggggcgtgcg tgggaagcgt tgcgtgaaga tgagatcgcc
    13561 tgccgttcgc tgggcttaag cccgcgtcgt atcaagctga ctgcctttac catcagtgcc
    13621 gcgtttgccg gttttgccgg aacgctgttt gctgcgcgcc agggctttgt cagcccggaa
    13681 tccttcacct ttgctgaatc ggcgtttgta ctggcgatag tggtgctcgg cggtatggga
    13741 tcgcagtttg cggtgattct ggcggcaatt ttgctggtgg tatcgcgcga attgatgcgt
    13801 gatttcaacg aatacagcat gttaatgctc ggtggtttga tggtgctgat gatgatctgg
    13861 cgtccgcagg gcttgctgcc catgacgcgc ccgcaactga agctgaaaaa cggcgcagcg
    13921 aaaggagagc aggcatgagt cagccattat tatctgtgaa cggcctgatg atgcgcttcg
    13981 gcggcctgct ggcggtgaac aacgtcaatc ttgaactgta cccgcaggag atcgtctcgt
    14041 taatcggccc taacggtgcc ggaaaaacca cggtttttaa ctgtctgacc ggattctaca
    14101 aacccaccgg cggcaccatt ttactgcgca atcagcacct ggaaggttta ccggggcagc
    14161 aaattgcccg catgggcgtg gtgcgcacct tccagcatgt gcgtctgttc cgtgaaatga
    14221 cggtaattga aaacctgctg gtggcgcagc atcaacaact gaaaaccggg ctgttctctg
    14281 gcctgttgaa aacgccttct ttccgtcgcg cccagagcga agcgctcgac cgcgccgcca
    14341 cctggcttga gcgcattggt ttgctggaac acgccaaccg tcaggcgagt aacctggcct
    14401 atggtgacca acgccgtctg gagattgccc gctgcatggt gacgcagccg gagattttaa
    14461 tgctcgacga accggcggca ggtcttaacc caaaagaaac caaagagctg gatgagctga
    14521 ttgccgaact gcgcaatcat cacaacacca ctatcttgtt gattgaacac gatatgaagc
    14581 tggtgatggg gatttcagac cgaatttatg tggtcaatca ggggacgcca ctggcaaacg
    14641 gtacgccgga gcaaatccgt aataacccgg acgtgatccg tgcctattta ggtgaggcat
    14701 aagatggaaa aagtcatgtt gtcctttgac aaagtcagcg cccactacgg caaaatccag
    14761 gcgctgcatg aggtgagcct gcatatcaat cagggcgaga ttgtcaccct cattggcgcg
    14821 aacggggcgg ggaaaaccac cttgctcggc acgttatgcg gcgatccgcg cgctaccagc
    14881 gggcgaattg tgtttgatga taaagacatt accgactggc agacggcgaa aatcatgcgc
    14941 gaagcggtag cgattgtccc ggaagggcgt cgcgtcttct cgcggatgac ggtggaagag
    15001 aacctggcga tgggcggctt ttttgccgaa cgcgaccagt tccaggagcg cataaagtgg
    15061 gtgtatgaac tgttcccacg tctgcatgag cgccgtgttc agcgggcagg caccatgtcc
    15121 ggcggtgagc aacaaatgct ggcgattggt cgcgcgctga tgagcaaccc gcgtttgctg
    15181 ctacttgatg agccatcgct tggtcttgcg ccgattatca tccagcaaat tttcgacacc
    15241 atcgagcagc tgcgcgagca ggggatgact atcttcctcg tcgagcagaa cgccaaccag
    15301 gcgctaaagc tggcggatcg cggctacgtg ctggaaaacg gccatgtggt gctctccgat
    15361 actggtgatg cactactggc gaatgaagcg gtgagaagtg cgtatttagg cgggtaataa
    15421 cacgttgatt gataaggagt caaaagactc ctttgagaca ggtgacaaat gtaaaattgc
    15481 ctgatgcgct gcgcttatca ggcctactgg gtgagttgca atatattgaa tttgcacgat
    15541 cttgtaggcc ggataaggcg tttacgccgc atccggcatg aaacgatgag taatctgtag
    15601 agtttgattc agaccttcta tttttccgct tatccgtgcc ccatctccca ttttccctca
    15661 cccacgccgt caccgccttg tcatctttct gacaccttac ttgcaggttt atgaagttgg
    15721 caccgccacc atgatggcat cgaaagccat taaaccggta tatgacgtgt ttaaagaggc
    15781 agggattcaa ttcgatgagt cgcagtttgt gccgacggtt tccggttact actccgacag
    15841 caaaacgggc cacttactct cccagccatt caacagctcg actcccgttc tctattacaa
    15901 caaagacgcc ttcaagaaag cggggttaga cccggaacag ccgccgaaaa cctggcagga
    15961 tctggcggac tcttccgcaa aactgaaagc ctccggtatg aagtgcggct acgccagcgg
    16021 ctggcagggc tggatccaac tggaaaactt tagcgcctgg aacggtctgc cgtttgccag
    16081 caaaaacaac ggctttgacg gcacggacgc ggtgctggag ttcaacaagc cggagcaggt
    16141 gaaacacatt gccatgctcg aagagatgaa caagaagggc gacttcagct acgtcggtcg
    16201 taaggatgaa tccaccgaga agttctataa cggtgattgc gcgatgacca ccgcctcttc
    16261 cggttctctc gctaatattc gtgagtacgc caaatttaac tatggcgtag gcatgatgcc
    16321 ttacgacgcc gatgcgaaag atgcaccgca aaacgccatt atcggcgggg ccagcctgtg
    16381 ggtgatgcag ggcaaaaata aagaaacgta taccggcgtg gcgaagttcc tcgacttcct
    16441 cgcgaagcca gaaaacgctg ccgagtggca tcagaaaacc ggttatctgc caatcaccaa
    16501 agcagcgtat gacctgaccc gtgagcaggg cttttatgag aaaaacccag gggcggatac
    16561 cgcgacgcgt cagatgctga ataagccgcc gttgccgttc accaaagggc tgcgtctggg
    16621 caacatgccg cagatccgcg tgattgtgga tgaagagctg gagagcgtgt ggaccggtaa
    16681 gaagacacca cagcaggcac tggataccgc cgttgagcgt ggaaatcagt tgctgcgccg
    16741 ctttgagaaa tcgacgaagt cttaatcagt gtaatgtcgg atgcgtttcg cttatctgac
    16801 ctggcatcgc gtgtaggccg gataagcgaa gcgcatccgg cacagttcag gaattaaccg
    16861 taatgtcatc atcccgtccg gtgttccgct cgcgctggct gccttatctg ctggtcgcgc
    16921 cgcagctcat catcaccgtt atctttttta tctggcctgc gggcgaagcg ttgtggtact
    16981 cgcggcaaag cgtcgatccg tttggtttct ccagccagtt tgtcggtctg gataacttcg
    17041 tcacgctgtt ccatgacagc tactatctag actccttctg gacgacgata aaattcagca
    17101 cctttgtcac cgtcagcggt ttgctggtat cgctgttctt tgcggcgctg gtggagtaca
    17161 tcgtgcgcgg cagccgtttc tatcaaacct tgatgctgct gccgtatgcc gtggctcccg
    17221 ccgttgccgc cgtattgtgg atcttcctgt ttaaccccgg tcgcgggctg atcacccatt
    17281 ttctcgctga gttcggctac gactggaacc acgcgcaaaa cagcggtcag gcaatgtttc
    17341 tggtggtgtt tgcctcggtg tggaagcaaa tcagctacaa ctttctgttc ttctatgccg
    17401 cgctgcactc cattccccgt tcgttgattg aagccgcagc tattgacggc gcagggccga
    17461 ttcgccgctt ctttaagatt gctctgccgc ttatcgctcc ggtgagtttc ttcctgctgg
    17521 tggtgaacct ggtgtacgcc ttcttcgaca ccttcccggt gatcgacgcc gccacgtccg
    17581 gcgggccggt tcaggccacc actacgctga tttataagat ctaccgcgaa ggctttaccg
    17641 gactggatct ggcttcgtct gccgcgcagt cggtggtgtt gatgttcctc gtcatcgtgc
    17701 tgacggtggt gcagttccgc tatgttgaaa gcaaggtgcg ttaccaatga ttgagaaccg
    17761 tccgtggctg acgatattca gccataccat gctgatcctc gggatcgcgg tgatcctctt
    17821 cccgctgtac gtggcgtttg tcgcggcgac gctggataaa caggccgtct atgccgcgcc
    17881 gatgacgctc atccccggca cacatctgct ggaaaatatc cacaacatct gggttaacgg
    17941 ggtaggcacg aatagcgcgc cgttctggcg gatgttgctt aacagctttg tgatggcgtt
    18001 cagcattacg ctcggcaaaa ttaccgtctc gatgctctcg gcatttgcca ttgtctggtt
    18061 tcgttttccg ctacgtaacc tcttcttctg gatgattttt atcaccctga tgctgccggt
    18121 tgaagtacgt atcttcccga cggtggaagt catcgccaac ctgaaaatgc tcgacagcta
    18181 cgccggttta acgctgccgc tgatggcctc ggcgaccgcc acgtttctgt tccgccagtt
    18241 ctttatgacc ctaccggatg aattggtgga agccgcgcgg atcgacggcg cgtcaccaat
    18301 gcgtttcttt tgcgacatcg tttttccgtt gtccaaaact aatctggcgg cgctgtttgt
    18361 gatcaccttt atctacggct ggaatcagta tttgtggccg ttgttgatta ttaccgacgt
    18421 tgatctcggc accaccgtgg cagggatcaa agggatgatc gctacaggcg aaggcaccac
    18481 ggaatggaac tcggtaatgg cggcgatgtt gttaacgctt atccctccgg tggtgattgt
    18541 tttagtgatg cagcgtgcct tcgtgcgcgg cctggtcgat agtaagaaat aagatggcag
    18601 gactgaaatt acaggcagta accaaaagct gggatggcaa aacccaggtc attaaaccgc
    18661 tgacccttga tgtggcggat ggcgaattta tcgtgatggt cgggccgtct ggctgcggga
    18721 aatcgacgct gctgcgcatg gttgccgggc tggagcgggt gacagaaggc gatatctgga
    18781 tcaacgacca gcgcgtgacc gaaatggagc cgaaagatcg cgggattgcg atggtgttcc
    18841 agaattacgc gctttatccg catatgagcg tagaggagaa catggcgtgg gggctgaaaa
    18901 ttcgcggcat gggcaagcag caaattgccg agcgcgttaa agaagcggcg cgcattctgg
    18961 aactggacgg tctgcttaag cgtcgtccgc gtgagctatc cggcggtcag cgccagcgtg
    19021 tggcgatggg ccgcgcgatt gtgcgcgatc cggcggtgtt cttgtttgat gagccgctct
    19081 ctaacctcga tgccaagctg cgcgtgcaga tgcgtcttga actgcaacag ttgcaccgtc
    19141 gcctgaaaac gacttcactc tacgttactc acgatcaggt tgaagcgatg acgctcgccc
    19201 agcgagtaat ggtgatgaac ggtggcgttg ccgaacagat tggcacacca gttgaagtct
    19261 acgaaaagcc cgccagcctg tttgtggcga gttttatcgg cagcccggcg atgaacctgc
    19321 tgacaggccg cgtgaataat gaaggcacgc atttcgaact ggacggcggt atcgtgctac
    19381 cgctaaacgg tggctaccgt cagtatgccg ggcgtaaaat gactctcggc attcgcccgg
    19441 aacatatagc gctaagctcg caggcagaag gtggtgtgcc gctggtgatg gacacgctgg
    19501 agatcctcgg cgcagataac ctggcgcacg gacgctgggg cgagcagaag ctggtggtgc
    19561 gactggcgca tcaggagcgc ccgacggcag gcagcacgct gtggctgcat ttggcggaaa
    19621 atcagctgca tctttttgat ggtgaaacag gacaacgagt atgagtaact ggccttatcc
    19681 ccgcatcgtc gctcatcgtg gcggcggtaa gctggccccg gaaaacaccc tggcggcaat
    19741 cgacgtcggg gcaaaatacg gtcataagat gatcgaattt gacgcgaagt tatcgaaaga
    19801 tggcgagatc ttcctgctcc atgacgacaa tctcgaacgc accagcaacg gctggggcgt
    19861 cgcgggtgaa ctgaactggc aggatttact gcgcgtggat gcgggcagtt ggtacagcaa
    19921 agcgtttaaa ggtgagccgc tgccgttgct ttcgcaggtg gcggaacgct gtcgcgaaca
    19981 cgggatgatg gcgaatatcg aaatcaaacc caccactggc actggaccat taacgggcaa
    20041 aatggtggcg ctggcggcac gccaactgtg ggccggtatg acgccgccgc tgctgtcatc
    20101 gtttgagatt gatgctttag aagctgcaca acaggcggca ccggaactgc cgcgtggttt
    20161 attgcttgat gagtggcgcg acgactggcg cgaactgacc gcgcggctgg gctgcgtctc
    20221 tattcatctc aatcataagt tactcgataa agcgcgagtg atgcagttga aagacgccgg
    20281 attacggatt ctggtttata ccgtcaataa accccagcgc gcggcagagt tgctgcgctg
    20341 gggcgtggat tgcatctgta ccgatgcgat tgacgtgatt ggtccgaact ttacggccca
    20401 atagttttca acggaatgtc aggctgcggc gtgctgggct gattcagcat gtcgccgttt
    20461 ctcctttccg gcagcatatg ctgctgattc aacgaactat ccggattacg gttgctgttt
    20521 aacatcccgc cgttggtgtt gggcagcatt tgctgccggg cgggatttcg ctcccccggc
    20581 tgtgactgca acacccgctg agaattattg tttatctggt tttctaaatg ctgctgttgc
    20641 aactgagttt gcgttttcag ttgctgattc agcatccctt tttgctggat ttgctgagtc
    20701 tgcatctggg tttgcatccg ctgctggctg ggtatctgat accccggctg gttagggttg
    20761 ttcagagtat taatgggctg tgcaaagccg acaaacggca ggagtgccgt aagaatcaga
    20821 agtcgtttca tcgcgtatcc tcctctgaag atatccttta agtttactcg cttcccgaca
    20881 aaacgatgat taattcagag ttatatacca ggcttagctg gggttgcccc ttaatctctg
    20941 gagaataacg atgataaaac cgacgttttt acgccgggtg gccattgctg ctctgctctc
    21001 aggaagttgt tttagcgccg ccgccgcgcc tcctgcgccg cccgtctcgt atggtgtgga
    21061 ggaagatgtc ttccacccgg tacgcgcgaa acagggaatg gtagcgtctg tggacgccac
    21121 tgccactcag gtgggggtgg atatcctcaa ggagggcggg aatgccgttg atgccgccgt
    21181 ggcggtgggc tacgcgctgg cggtaacgca tccgcaggca gggaatctgg gcggtggtgg
    21241 ttttatgtta atccgctcga aaaatggcaa taccacggct atcgatttcc gcgaaatggc
    21301 acccgccaaa gcgacccgcg atatgttcct cgatgatcag ggcaacccgg acagcaaaaa
    21361 atcactcact tcgcatctgg cttccggcac accgggtacg gtagcaggtt tctcgctggc
    21421 gctggataaa tacggcacca tgccgctgaa caaagtcgtg cagcccgcgt ttaaactggc
    21481 acgcgatggt tttatcgtta acgacgcgct ggctgacgat ctcaaaacct acggtagcga
    21541 agtgttgccg aatcacgaaa acagtaaagc tatcttctgg aaagagggcg agccgctgaa
    21601 aaagggcgac acgctggtgc aggcgaacct ggcaaagagc ctggagatga ttgctgaaaa
    21661 cggcccggac gaattctata aaggcacgat tgcggaacag atcgcccagg agatgcagaa
    21721 aaacggtggc ttgatcacta aagaagattt agcggcctat aaagcggtcg aacgcactcc
    21781 gataagcggc gattatcgcg ggtatcaggt ttactccatg ccaccgccat cctccggcgg
    21841 gatccatatc gtacaaatcc tcaatattct ggaaaacttc gatatgaaga aatacggctt
    21901 tggcagcgcc gatgcgatgc aaatcatggc agaagcggag aaatacgcct acgccgaccg
    21961 ctcggaatat cttggcgacc cggattttgt caaagtcccg tggcaggcac tgaccaataa
    22021 agcctatgcc aaatccattg ccgatcaaat tgatatcaac aaagcgaagc cgtccagtga
    22081 gattcgccct ggcaagcttg cgccttatga gagtaatcaa actacccatt actctgtagt
    22141 ggataaagac ggtaacgcgg tggcggtgac ctatacgctg aacaccacct tcggtacggg
    22201 cattgtcgcg ggcgagagcg gtattctgct taataatcag atggatgatt tctccgccaa
    22261 accgggcgta ccgaacattt acgggctggt gggcggtgat gccaacgccg tcgggccgaa
    22321 taaacgtccg ctgtcatcga tgtcaccaac cattgtggtg aaagacggta aaacttggct
    22381 ggttaccggt agtcctggcg ggagtcggat tattaccacc gtgct
//
LOCUS       DAZOYL010000035        21642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZOYL010000035 DAZOYL010000000
VERSION     DAZOYL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.035"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHUOY_000996"
     CDS             complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHUOY_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR0681842.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPRSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1344..2996
                     /gene="ushA"
                     /locus_tag="ACHUOY_000997"
     CDS             1344..2996
                     /gene="ushA"
                     /locus_tag="ACHUOY_000997"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR0681843.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHUOY_000998"
     CDS             complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHUOY_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR0681844.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3716..4510)
                     /locus_tag="ACHUOY_000999"
     CDS             complement(3716..4510)
                     /locus_tag="ACHUOY_000999"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR0681845.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4648..4989
                     /locus_tag="ACHUOY_001000"
     CDS             4648..4989
                     /locus_tag="ACHUOY_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0681846.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHUOY_001001"
     CDS             complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHUOY_001001"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR0681847.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7970..8902
                     /gene="glsA"
                     /locus_tag="ACHUOY_001002"
     CDS             7970..8902
                     /gene="glsA"
                     /locus_tag="ACHUOY_001002"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR0681848.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8905..10197
                     /locus_tag="ACHUOY_001003"
     CDS             8905..10197
                     /locus_tag="ACHUOY_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR0681849.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10228..10325
                     /locus_tag="ACHUOY_001004"
                     /pseudo
     CDS             10228..10325
                     /locus_tag="ACHUOY_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10322..10729
                     /gene="cueR"
                     /locus_tag="ACHUOY_001005"
     CDS             10322..10729
                     /gene="cueR"
                     /locus_tag="ACHUOY_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR0681850.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10730..11188)
                     /locus_tag="ACHUOY_001006"
     CDS             complement(10730..11188)
                     /locus_tag="ACHUOY_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR0681851.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11185..12102)
                     /locus_tag="ACHUOY_001007"
     CDS             complement(11185..12102)
                     /locus_tag="ACHUOY_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0681852.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12248..12925
                     /gene="fetA"
                     /locus_tag="ACHUOY_001008"
     CDS             12248..12925
                     /gene="fetA"
                     /locus_tag="ACHUOY_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR0681853.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12912..13691
                     /gene="fetB"
                     /locus_tag="ACHUOY_001009"
     CDS             12912..13691
                     /gene="fetB"
                     /locus_tag="ACHUOY_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR0681854.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHUOY_001010"
     CDS             complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHUOY_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR0681855.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHUOY_001011"
     CDS             complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHUOY_001011"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0681856.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHUOY_001012"
     CDS             complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHUOY_001012"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR0681857.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHUOY_001013"
     CDS             16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHUOY_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR0681858.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHUOY_001014"
     CDS             16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHUOY_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR0681859.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHUOY_001015"
     CDS             complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHUOY_001015"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR0681860.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHUOY_001016"
                     /pseudo
     CDS             complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHUOY_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5014 a         5684 c         5800 g         5144 t
ORIGIN      
        1 gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa gcaaaaccga
       61 aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg tagaccagaa
      121 tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc cccgtccagt
      181 gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata gagccccaaa
      241 tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta cctgccgcaa
      301 cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat ccgaattttt
      361 gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat ttcgagaaaa
      421 tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt ggattgatta
      481 tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa cggctaattt
      541 gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg ttgcctttgc
      601 cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca aagttgccgc
      661 cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata cgggctacgc
      721 gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc gccagcagaa
      781 ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta aagcacatgc
      841 caattggcaa cgaccgcggc atcggatatt tatcggtcca gtagccgacc actggttgca
      901 gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata aatgtcagag
      961 aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc atgtcgttca
     1021 gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc ttggtcgttg
     1081 acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga aagtcacttt
     1141 ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact aacatacctg
     1201 tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct ggatgcagat
     1261 ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt tcaacacgtt
     1321 gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg ttagcgctgt
     1381 taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat aaaacctaca
     1441 aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat gaatatggcg
     1501 aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag gttgcggctg
     1561 aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg cccgagtctg
     1621 acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat gacgcgatgg
     1681 cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag gaaaagtggg
     1741 ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag cgcctgttta
     1801 aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg ctgacaaccg
     1861 atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt cgtaagcccg
     1921 ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca gacattatta
     1981 tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac gcaccgggcg
     2041 atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc ggtggtcact
     2101 cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac gtgccgggta
     2161 cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat gagtggggca
     2221 aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg gttaactacc
     2281 agctgattcc ggtgaacctg aagaagaaag tgaccaggga agacgggaaa agcgagcgcg
     2341 tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat ctcgctgtta tcgccgttcc
     2401 agaacaaagg caaagcgcag ttggaagtga aaataggcga aaccaatggt cgtctggaag
     2461 gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt ctggcagccc
     2521 aaatggatcg cactggtgcc gactttgcgg tgatgagtgg aggaggaatt cgtgattcta
     2581 tcgaagcagg cgatatcagc tataaaaacg tactgaaagt gcagccattc ggcaatgtgg
     2641 tggtgtatgc cgacatgatc ggtaaagagg tgattgatta cctgaccgcc gtcgcacaaa
     2701 tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg gcgaaagacg
     2761 gcaaactcaa cgacctgaaa atcaaaggcg aaccggtcga tccggcgaaa acttaccgta
     2821 tggcgacatt aaacttcaat gccaccggcg gtgatggcta tccgcgcctt gataacaaac
     2881 cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat atccagaaaa
     2941 gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg cagtaatccg
     3001 aaagtgccgg atatttgcat ccggcacaat gcttaatcgc ggcgggcgat atcagcaaat
     3061 ttggcatcga ggatcttcgc cagatcgcct gccgccagtt cgatatccag tccgcgcttg
     3121 ccgccagaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat cgtcggcaga
     3181 cgttttttct gccccaatgg gctaatcccc ccaaccaggt atcccgtcga acgctgcgcg
     3241 gccatcggat cggccatctc aactttcttg gcacccagcg cttttgctac ttttttaaga
     3301 tccagttgac ctgcgaccgg cgtaacggcc actgccagat gtttcatatc accgttcact
     3361 gccaccagta gcgttttgta gacttgatcc ggattcaaac ctaatttttt gacgacttca
     3421 tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa cgaaatcttg
     3481 tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta aacaaaaaaa
     3541 cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc taagagtatt
     3601 ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt gacggcataa
     3661 taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca tttttttagc
     3721 gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc gcgaccacat
     3781 atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc tgatgcatca
     3841 gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta ttttgcggag
     3901 gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta tgccagtggg
     3961 tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac atggtaatct
     4021 ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc gcctgcaata
     4081 actggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc gtcgcctgaa
     4141 gaaccatcgc gatttgccac agcggttgag tagaaaagag cgacggagaa atgcccatct
     4201 cctggctaat gtgctgcaga ttttggagtt gctcctcgtt aatgcgctct tccagcgcct
     4261 cgcaagtagg caaattagca aaaggcgtat cgctggtgga aacatccgcc tcgacgatca
     4321 gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc atatcgtggc
     4381 tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg gtgatatcga
     4441 tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt acccggtaca
     4501 acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga gggatggcgc
     4561 aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct tgcatcgcta
     4621 ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc gacgacaccc
     4681 ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa tgagttagca
     4741 gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa tcgtaaactc
     4801 accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga ttttaggcta
     4861 aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac ccaggaagaa
     4921 ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg tgcaaaaaag
     4981 gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt cacgccgcat
     5041 ccggcatccg agcaccattg cctgatgcga cgcttacccg tcttatcagg cctacaaacc
     5101 tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc atgcgtcgat
     5161 gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc ttcggtttaa
     5221 accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc attgccgctc
     5281 cggcaactac cgggttaagc agtgttcccg taaacggcca caaaataccg gcggcgaccg
     5341 gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg ttgcgcagcg
     5401 ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc atcagggtaa
     5461 tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg acatccgcct
     5521 gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga cgtccttcac
     5581 tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca atcacttcat
     5641 caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg gtcaacatca
     5701 ccagacgata tcccgtttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg
     5761 gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc acaggcgttg
     5821 ctccttgcga tgcctgagca gtaatctccg cttcgatagc tttggtacca acctgttgct
     5881 catttaacag cgcctgattg cccagcaata acgcatgacc ttcagcttca ccgctcacgc
     5941 ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag ctgcatatca cctgctttat
     6001 cgaggatcgc ccgtgccagc ggatggctgg aaccttgctc cagtgccgcc gccagacgca
     6061 atgcctgcgc ttcatcaaca tcagcaaacg ttttcactgc gacaacctgc ggcttccctt
     6121 cggtcagcgt cccggtttta tcgaacacga tggtatcgag tgtactggcg cgttgcagcg
     6181 cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg gaaataatcg
     6241 acatcggcgt cgccagcccc agcgcacacg gacaggcgat aatcagtacc gtggtggcaa
     6301 tcaccagggt ataaacaatt tgcggtgccg gaccaaagaa gtaccagatt gccgcactga
     6361 ccagcgcaat caccaccacc acaggtacaa atacggctga gattttatcc gccagctgac
     6421 cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatccgt gacagcgtgg
     6481 tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc actgtcccgg
     6541 catggacgct ttcgccttcg cctttttgct gtgggattgg ttcgcccgtc agcatcgctt
     6601 catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga tcgccggtcg
     6661 tcaggcgcag taacatccct ggctgaactt ccgccagcgg cacgcttttt tcaccatctg
     6721 cggtgaccac tcgcgccgtc ggtggggtta aatcgagtaa tttctccagc gccttagaag
     6781 aacgctgacg tgcgcgcgct tccagcatat ggccgagatt aatcagaccg ataatcatcg
     6841 cactggcttc atagtaaaga tgccgcgctt ccatcgggaa ccactgcggc cacaggttga
     6901 cgctcatcga atagagccac gccacacccg ttcccagcgc caccagcgta tccatcgtcg
     6961 ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg gcgaaaacca
     7021 tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca gcggtgacca
     7081 tcatgttatc gccgatcatc ccccagacca ttaccgggat acccacagcc agtgcaacaa
     7141 ttgcctgcca gcggaagcgc ttcatcgtag ctacggcggt ttcttgctgg cgctcgcggc
     7201 gtttagcgtc atcttcaatc gcttccgcac cgtagcccgc tttttccacc gcctgcacta
     7261 aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg
     7321 cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca cagctggcgc
     7381 agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa
     7441 gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag
     7501 cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt
     7561 ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgcc tgctcaacat
     7621 ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag gacaggccgt
     7681 ccagggtcag gtcgatagtt tgtgacataa aacactcctt taagacagtt ttgactggct
     7741 gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa ccaggcgggt
     7801 aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac atttatttct
     7861 ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg aaaatcggca
     7921 ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga tgttagatgc
     7981 aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac ttaacggcgg
     8041 acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg cggcagtggc
     8101 tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc gctttgcact
     8161 ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc
     8221 ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag ttatcgcctt
     8281 agagttgcat ggcggcaaac cgctgtcgcc actggtaaat gctggcgcta ttgccaccac
     8341 cagcctgatt aacgctgaaa atgttgaaca acgctggcag cgaattttac atatccaaca
     8401 gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg aacaaacaac
     8461 caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc tctattgtga
     8521 tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca atactgttga
     8581 actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga cgcataaacg
     8641 cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg aagggctgta
     8701 tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg
     8761 cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac caccgctgga
     8821 cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg
     8881 ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg taacaaaccc
     8941 ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc ggggatcttc
     9001 gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctgggtcgc ctttgctttt
     9061 ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc gagctatccc
     9121 agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt cttttcgcta
     9181 gcgctctcgt tgctgtacct gttgacgctg gcggtgagca tcgccatggt tgcccgtgct
     9241 tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca ccttattttg
     9301 ctctacgcgt tggggatcat tgcggtgatg acgcttttta actccttaag caaccatgcg
     9361 gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt attgctgatt
     9421 attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc ccccagctcc
     9481 ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt tggcatgatg
     9541 gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc gtttctggtg
     9601 gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct tagcgatgta
     9661 tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc ttctccgctg
     9721 ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac ggcttcagcc
     9781 attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag cgaacgcgaa
     9841 ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa cattattgtc
     9901 gtggtgttga ttatgctgat gacggcggcg ctaaatttag gctcactcgc cagtgttgcc
     9961 agcgccacct ttttgatttg ctacctggcg gtgtttgtgg tggcgatccg cctgcgtcat
    10021 gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt gttggtgatc
    10081 gttggcttta tctacagtct gtggtcccag ggtagccgtg cgttgatatg gattattggc
    10141 tcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac cgtataacat
    10201 ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg cactggcaaa
    10261 aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg ttgcgcgaac
    10321 gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca ttcgcttcta
    10381 tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc gcacctacac
    10441 gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg gctttaacct
    10501 ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca gcgccgacgt
    10561 caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg agctgcaatc
    10621 catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca gcgccgactg
    10681 cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat taagacgaga
    10741 cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc gtacctgcac
    10801 cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata tgaccgcgcc
    10861 cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc ccgcgctggt
    10921 ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat ttccaccaca
    10981 gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc caacccagcg
    11041 gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac aacaaataac
    11101 catttccgcc cagcatctcg gctgccagca gcaaaccgcc gagactgagc cagaaaatat
    11161 gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc tgtctttcac
    11221 cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg gcatcatcac
    11281 tactttgctg ttactggagg taccgatctg ctgtaacgct tcggtgtatt tctgcgctac
    11341 gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca ttttggtggc
    11401 gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg
    11461 ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga ggatttccgc
    11521 ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg ctttcatctg
    11581 cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt caatacgggt
    11641 gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc gtgagttgat
    11701 gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg tacggatgtt
    11761 agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag ccgcgcgcgg
    11821 cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt tatctttcga
    11881 gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct tgcgaccaat
    11941 gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg tatagcgacc
    12001 aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg cgccgacaat
    12061 gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa cctcctgttg
    12121 taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg attactgaaa
    12181 gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc agaaaaaggg
    12241 aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat atctggcggg
    12301 tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat ttaagttaat
    12361 taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt cattgatcag
    12421 cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa agccagaaat
    12481 ctaccgccaa caagtctctt actgcgccca gacaccgacg ctgtttggcg atacggtata
    12541 cgataatctg atctttccct ggcagatccg taaccggcag cctgacccag ccatttttct
    12601 cgattttctc gaacgcttcg ccttgccgga cagcattttg acgaaaaata tcgccgagct
    12661 atctggtggt gaaaaacaac gcatctcact gattcgtaac ctgcaattta tgccgaaggt
    12721 tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata acgttaatga
    12781 gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga cacacgataa
    12841 agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg gagaaatgca
    12901 ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg gcattaatgc
    12961 tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag aaagatattc
    13021 tctggagcgt cggacgagcg ataattcagc tgattattgt cggctatgtg ctgaagtata
    13081 ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc tgctttaatg
    13141 cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc tcatcgttta
    13201 ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca gggtcgattg
    13261 aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac gccatggtag
    13321 cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag caacagattc
    13381 aggagaaact gagtcttggt gcgacgccga agcaggcttc agcgatattg attcgcgaca
    13441 gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc ttagtgagtt
    13501 taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag gcgattaaat
    13561 atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc attattgcct
    13621 gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg acgcaattga
    13681 agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc
    13741 gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc gacgccagtg
    13801 catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc tgaccgtctg
    13861 cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct tcattgcgcc
    13921 caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct ggattctccg
    13981 ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc agcagttcga
    14041 tttgcgccac catcccctgg tagcgggtgt cctgatcctg taacggaatg gttttcagca
    14101 ccgcttctgc atcttcagaa cggttcagcg caatcagcgt ttctgccaac agcaggccga
    14161 tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg gcatcggtga
    14221 aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct tcgcgcggca
    14281 gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg aagccatcta
    14341 ccggttgccc gttctggaac agatacaccg tcggaatcgc gcgcagacca aactgggcgg
    14401 caatcatctg ctccgcatcg cagtccagct tcgccagaat aaattgcccg ttgtactgcg
    14461 ccgcaaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt tcagaccaaa
    14521 aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg ttagattcgt
    14581 taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat attttctttg
    14641 acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc atcacgcgcc
    14701 ccggcagcag gcgcttaagc accattactg cccaagtcac cagcgttacc ggatagcgca
    14761 tcttcggctt ctcgctaata aaagcatggc gtactttgtc caccaccgct tccggtccca
    14821 acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc gtctggttga
    14881 cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact ttaattccgc
    14941 tgtggcgtag ctccatgcgc agcgcgtctg accatgcctc cagcgcatat ttactggccg
    15001 cgtaagcgcc acgacccggt gtggagatta atcccatcac cgatgatgtc atcacaatac
    15061 gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga
    15121 aaaagttggc ggaaaactgc tgttccatct gcgcgcggct gatggtggaa agggggccat
    15181 acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc agggcgatca
    15241 cttcgtcggc tgcgcgatca acactttctg gcgaatccag atcgatcaac acgccggtaa
    15301 atcccatgct gttcatacgc tcaacatcat ccggtttccg gcaacctgcc agcacatgaa
    15361 aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa catccggtaa
    15421 ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta tgagtcatga
    15481 tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg
    15541 ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc ttccataaaa
    15601 aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc gctaaaggct
    15661 tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa tggttcagcg
    15721 ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg ctgtggctga
    15781 aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg ctgatgctgt
    15841 ttcagcagag ccggaaggcg cgccagtcct tgttgcgagg tgtcgccgct gatgctggca
    15901 ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg
    15961 gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg
    16021 gcggcacgga aggttaacag ggccaggaac aggaagggca aatgccagcg gaaaacattg
    16081 ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca
    16141 ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc gagtcgggat
    16201 cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc agtggcgaag
    16261 tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg
    16321 cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg
    16381 aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg
    16441 cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt ccggcacagc
    16501 tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt cgacctgatg
    16561 tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat aaaattgccg
    16621 acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg acccacgacc
    16681 tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg
    16741 aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat taattgtctg
    16801 gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat
    16861 ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag
    16921 ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg
    16981 caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc tggcgaacgt
    17041 caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc cccctggcct
    17101 gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa
    17161 aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca
    17221 ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc
    17281 ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt
    17341 cggcggcaac gagaaccagc tcgacggcta tgataaatgg ttgttacctc agcttaaacc
    17401 cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat cgatggaacg
    17461 ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag tggcagcggt
    17521 ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga tcctcaaaac
    17581 gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct
    17641 gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg tgttgatggt
    17701 gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc cgtggctgtg
    17761 ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat accgcttgtt
    17821 gctggcaacg cagcctttac gcgtattacg taatgatgtg gtagcgaacg tctggccgct
    17881 gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg
    17941 tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg
    18001 tgtgctgggc tggatgcagc tgaatgtact tcgccgcatg acgctgaaat cgctacctct
    18061 gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc
    18121 attttcgctc tcctttatgc tgctggcgct gttgctggta ttgcgtggcg atctgctcga
    18181 ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta acatcgccac
    18241 agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc cggaatcatt
    18301 ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag aaggtaatga
    18361 agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc ccgatcataa
    18421 tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct
    18481 ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg atacccagga
    18541 gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt
    18601 ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc ttaccagttt
    18661 ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc cgaccattag
    18721 cctgttagat attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg
    18781 ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt tgctggcaca
    18841 ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca cactcggtgc
    18901 ggggaaaaaa ctgctgcgta ccacgttgtg gtgtgagttc gccatgcttg ggtttgtttc
    18961 tggcctggta gccgcaattg gtgcggaaac ggcgctggca gtgttgcagg cgaaagtgtt
    19021 tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcact
    19081 gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta aggcgctgtt
    19141 caggcagttt gcggggtgat gacaggtgtt agattgcctg atgcgctacg cttatcaggc
    19201 ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat aaggcgttta
    19261 cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca actgtcatgt
    19321 cggtttttta tatctgtatt ctggtgcaac cattctgttc tcacgacaat cgccgtttca
    19381 gaataccgcc catcactgcg tcttaaccca ttccgccact tccgcccact caccgcgaaa
    19441 gacaactttt tccgcttttt tctcaagctg atagcgatac atcgggtcgt aatattcttt
    19501 aagtaacggc accagccagg ccagatgacc gtcggtgctg tcggtggtga gttgcgttgt
    19561 cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca gccgacgctt
    19621 aatagccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct gttcgtcgcc
    19681 gtacgcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcgttca ggcgctcaag
    19741 acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag
    19801 gcattccggc aggtgattcg aaccgatcat ccggctttcg tcttccagca cccacaggcg
    19861 caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt caaaactcgc
    19921 ctggctaagt tgtggttgta aggtgcgacc aaacgccgaa ccgcgatgac gcgctaaccc
    19981 ttccagatca acaccgttcg gctgttgctg cactaacagc gttttaccgc tgccggtaca
    20041 accgccaatc agcactatcg gtttttgtgc cagttcaata gtcgcctgaa tcgcggcttg
    20101 gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctg catacaacca
    20161 gctttgcaca atatgtgagc gctgaccgcc acgggcgcag cagagaatac cttgcggatt
    20221 ttgcaggcac gctgcccgcc aggcgtccat gcgctgctga cgaatttcac ccgccaccag
    20281 tttatgcccc agcgccagcg ccgcgtctga gccttgctgt ttataacaag tgccaacggc
    20341 ggcgcgttca tcgttattca ttaacggcag gttgatagcg gcgggcattg cgccttgctc
    20401 aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca gggcacgata
    20461 gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc
    20521 tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt
    20581 tctaaaaatc cgctgatttc cggcctgcgc tgggtaaaca aggtcacaat atcttctacc
    20641 gctttgccgc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt
    20701 gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag tgattttggc
    20761 aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat atcgggaaca
    20821 ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt tcgggcgctg
    20881 tcttcaatat tgaccgccgg aaagcgccgc aactgcgctt ctgttagcgg ctcttcaacg
    20941 ttcgccagcg gatgatccgc cgccatgaca aagcgccatt gcaccgatcc taagggatca
    21001 agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct
    21061 tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg aaactgggta
    21121 aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg gttatagagc
    21181 aggttgttga tgacaatatt cacctagcgt tccacgccat cattcacctg ttgcagctcg
    21241 ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag atgctcgcca
    21301 gccgctgtca acgtcacgct gcgagtcgtg cggaaaaaca gcgccactcc ggtattctct
    21361 tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg ttctgccgct
    21421 tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac
    21481 atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc tctgatttat
    21541 cagtattttt acatgataac cctgttcaat ttgtggacta aatctagttt tggaaaaata
    21601 ttccaacttt tgtattgatg ttgttctctt aaggttttag at
//
LOCUS       DAZOYL010000036        21465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZOYL010000036 DAZOYL010000000
VERSION     DAZOYL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.036"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            30..554
                     /gene="sat2"
                     /locus_tag="ACHUOY_001017"
     CDS             30..554
                     /gene="sat2"
                     /locus_tag="ACHUOY_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0681861.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            623..1120
                     /locus_tag="ACHUOY_001018"
     CDS             623..1120
                     /locus_tag="ACHUOY_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681862.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1181..1552
                     /locus_tag="ACHUOY_001019"
     CDS             1181..1552
                     /locus_tag="ACHUOY_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681863.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            1962..2339
                     /locus_tag="ACHUOY_001020"
     CDS             1962..2339
                     /locus_tag="ACHUOY_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0681864.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            2362..2514
                     /locus_tag="ACHUOY_001021"
                     /pseudo
     CDS             2362..2514
                     /locus_tag="ACHUOY_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(2570..4186)
                     /locus_tag="ACHUOY_001022"
     CDS             complement(2570..4186)
                     /locus_tag="ACHUOY_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0681865.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(4187..5713)
                     /locus_tag="ACHUOY_001023"
     CDS             complement(4187..5713)
                     /locus_tag="ACHUOY_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR0681866.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(5716..7383)
                     /locus_tag="ACHUOY_001024"
     CDS             complement(5716..7383)
                     /locus_tag="ACHUOY_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0681867.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(7380..9488)
                     /locus_tag="ACHUOY_001025"
     CDS             complement(7380..9488)
                     /locus_tag="ACHUOY_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0681868.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(9475..10296)
                     /locus_tag="ACHUOY_001026"
     CDS             complement(9475..10296)
                     /locus_tag="ACHUOY_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0681869.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(10456..12285)
                     /gene="glmS"
                     /locus_tag="ACHUOY_001027"
     CDS             complement(10456..12285)
                     /gene="glmS"
                     /locus_tag="ACHUOY_001027"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR0681870.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(12447..13817)
                     /gene="glmU"
                     /locus_tag="ACHUOY_001028"
     CDS             complement(12447..13817)
                     /gene="glmU"
                     /locus_tag="ACHUOY_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR0681871.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(14168..14587)
                     /locus_tag="ACHUOY_001029"
     CDS             complement(14168..14587)
                     /locus_tag="ACHUOY_001029"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR0681872.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(14608..15990)
                     /gene="atpD"
                     /locus_tag="ACHUOY_001030"
     CDS             complement(14608..15990)
                     /gene="atpD"
                     /locus_tag="ACHUOY_001030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR0681873.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(16017..16880)
                     /gene="atpG"
                     /locus_tag="ACHUOY_001031"
     CDS             complement(16017..16880)
                     /gene="atpG"
                     /locus_tag="ACHUOY_001031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR0681874.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(16931..18472)
                     /gene="atpA"
                     /locus_tag="ACHUOY_001032"
     CDS             complement(16931..18472)
                     /gene="atpA"
                     /locus_tag="ACHUOY_001032"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR0681875.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(18485..19018)
                     /gene="atpH"
                     /locus_tag="ACHUOY_001033"
     CDS             complement(18485..19018)
                     /gene="atpH"
                     /locus_tag="ACHUOY_001033"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR0681876.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(19033..19503)
                     /gene="atpF"
                     /locus_tag="ACHUOY_001034"
     CDS             complement(19033..19503)
                     /gene="atpF"
                     /locus_tag="ACHUOY_001034"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR0681877.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(19565..19804)
                     /gene="atpE"
                     /locus_tag="ACHUOY_001035"
     CDS             complement(19565..19804)
                     /gene="atpE"
                     /locus_tag="ACHUOY_001035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR0681878.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(19851..20666)
                     /gene="atpB"
                     /locus_tag="ACHUOY_001036"
     CDS             complement(19851..20666)
                     /gene="atpB"
                     /locus_tag="ACHUOY_001036"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR0681879.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(20675..21055)
                     /gene="atpI"
                     /locus_tag="ACHUOY_001037"
     CDS             complement(20675..21055)
                     /gene="atpI"
                     /locus_tag="ACHUOY_001037"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR0681880.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         5498 a         5551 c         4839 g         5577 t
ORIGIN      
        1 gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct gagcaggtgg
       61 cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat gtgcacctat
      121 ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag gattacatct
      181 cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac caagagcttg
      241 tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa cacattgttg
      301 tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt gcgaaaaagt
      361 gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac aatgtacctg
      421 cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg ttcacgtata
      481 aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc tcgggagcac
      541 aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc ttaattcagg
      601 agttagagag ctggggaaga ctatgcgcga tctgttgaag gtggttctaa gcctcgtact
      661 tgcgatggca tcggggcagg cacttgctga cctgccaatt gttttagtgg atgaagctcg
      721 tcttccctat gactactccc catccaacta cgacatttct ccaagcaact acgacaactc
      781 cataagcaat tacgacaata gtccatcaaa ttacgacaac tctgagagca actacgataa
      841 tagttcatcc aattacgaca atagtcgcaa cggaaatcgt aggcttatat atagcgcaaa
      901 tgggtctcgc actttcgccg gctactacgt cattgccaac aatgggacaa cgaacttctt
      961 ttccacatct ggcaaaagga tgttctacac cccaaaaggg gggcgcggcg tctatggcgg
     1021 caaagatggg agcttctgcg gggcattggt cgtcataaat ggccaatttt cgcttgccct
     1081 gacagataac ggcctgaaga tcatgtatct aagcaactag cctgctctct aataaaatgt
     1141 taggcctcaa catctagtcg caagctgagg ggaaccacta gtgtcatacg aacctccaag
     1201 agacggttac acaaacgggt acattgttga tgtcatgtat gacaatcgcc caagtaagta
     1261 tccagctgtg ttcagaacgt acgtccgaat tccagactat gtcgaactag ataacgcgga
     1321 ggagctggcc gacgcaatca ttcgagaggt tgagttaaag gaggcatggc tcacaagggt
     1381 cttggggaac tcagccgatc ctgacgcttc tgtgggcaag tgggaccggc gcctagcgca
     1441 gtgcggtggt attgatgaga tgtcaagtgg ggtgcaggca acatacttca gtccaaacgg
     1501 tcagctggtc ggcttagtgg catacattga tgccgaatgg atggatgaat agctctgacg
     1561 ttacgattca ggtgagacaa ccagatgggc attgcgtaca agctcagttg ccaccgatgt
     1621 ggctacgagt ctgatcttct ttacctaggc caaggtatgg cgatgcttcc tgagcaaata
     1681 gtcggaacgt gcacatgctg cgacaatcta acgacgattt ctataaatga aagctacgga
     1741 gtatgctcac tttgtggggc taaggatcgt gtgatattta caagctatca gcatgaggca
     1801 aggccaagaa atccctgcta tctagagaga agtttcaaat atcaatgccc aagatgccat
     1861 gccttcagta tggagcctcc ggatctaccg gagatgcttt gggactaatg ctccgctttg
     1921 cctcgggtat ccgttcgacc tatgtttaag aggaaacgca cgtgtctgcc gccaaagctc
     1981 ttgcctatgc actttgtgac aagttggaaa gcaaaaaagt tgatgaagtc gtacgaaatc
     2041 ttgtttctag cggctggaat attagggaag tggcctggga agcttcgtcc tttgatcatg
     2101 aaatgcccta ctttgacacg gggcgggact tcgatattgg atttcctgac acgctgtcat
     2161 ccaagattga gtttttgacc gcgaatggat caatcgtcca agcacgtgct cgcctgcact
     2221 tcaagcaggc tctaattttt gccaaggcgt ccaaacatgt cgccgcgctc aagaactcat
     2281 tggattacta ctatggatcg tctgtaatcc cccctcaaaa ccacggaggc tatttgtaga
     2341 aaattccatt aatatgaact caaggtgttc cctacggacg aatcagcgat gaaggtggtg
     2401 tacctggcta tccaggcggc gaccaagaaa tggacgatgc cgatccgcaa ctggaagccg
     2461 gccatgaacc gttttatgat cgaatttggt gaccgtttaa atggccacct ttaactgacc
     2521 ggcaaaagca cttacacaaa agggtttaca ggctctttca gtcattcctt taatgcgtaa
     2581 attgctctct aaccactctc tcagcccacc cttccatatc ctctctcgat acttgcagct
     2641 cccccaaccc ctttggatga ttcacccctc tatgcccgtc ttgtccatat aattgatcaa
     2701 acaaactatt tggccaattc aatgagctct tcaccactcc acgccgtatc ttttcaaaat
     2761 cattccgcca tgtttcgcta tcaacgccac tcaacacttt ggtagaaagc attttcaccc
     2821 catccgatgc atccacctct agcaatacaa attcactgcc attacgttgc accccaacgg
     2881 ctttaatcac tcttggtgag ccatctttac acagatgtaa tcggctacgc ccaacttttg
     2941 gcaaaaccaa ggtttcttca aaaagcaccc gacatgcaaa tttagttttt agaattgaaa
     3001 gtagctcatc aaaagcagca aatcgattag caaaaataga gttgtaattg gttgcatcct
     3061 gcttcccacc cacatctgcc gctgctagga caccacctaa gtgtggctca tcagtactaa
     3121 ccaaattact attttcactt gttgttgctt cttctttttc taaaatagtt tggctacttt
     3181 ttcttgccct gcgagatttt tcaattcgac taggtctact aaaacttatc catgtcggct
     3241 cagcctctat gactcgtgtg tctgtgtcta tattggctgt ttcctcatcg tccaactgta
     3301 gatgctcatc atcaacaact gactctgttg aaaccgctat gtgctgcgta ctaccatcac
     3361 ctgcctcctt ttcctgaaaa gaggcatggc taatagctgt tgtgctagga agcatagcgt
     3421 tgatttctaa acctactatt tcctcaacta aataatcctt atcctcgtta gaagaacgtc
     3481 cttttacatg taatttccaa ccctgcatgg gcggagggtc aaagctaaag caccagcttt
     3541 caacgccgtt cttaatttct ctattttgtt gatagtgcct aaaaatactt tcatacgaat
     3601 ccataacatc ttgatccgaa aacaaccaaa ccaaaagctg cactacggcc gactgctcta
     3661 acgccccttt aggaaacgat gaactgggta agatccttat ctctaaatga tctcgctcaa
     3721 cctcatactg aacgtcgaac tcttgctgta aagcggtact gctcaaacag cttcgacaga
     3781 aataggagtt aattaaaaat aatgaccgag ctagctctaa ctgtgggatg tgataacgag
     3841 tcttgctata tggtgtttca gcttcgtaaa taaatgaact ctgcgctcca tccttgttgc
     3901 gtacggaagc aaaggacgag agattaggaa aatcgattat tttggctcgt tgaagatccg
     3961 atgcttcaaa ctcaatcaca cgatccgcct tatttatttg ctttgttgta ctgttaacca
     4021 ccttagctcg acttaataaa ggcaaatgag taaatcgagt ccatttccgt tcttgcattg
     4081 gattaaacca aacaaaaata cgccactcct tatgacccga gttacggaat aaatgaccaa
     4141 tatgtacaac ctgcacattg tcattaaatg tagctagcct aaccactcac tcttccccat
     4201 aaaccatttc caagaatctt tgtgcttcct cagttatccg ctctttagat aacgttgcac
     4261 ttcttaataa tcgccagcgc ctaagctcaa catcttcctg tttaagctta ataaaagctt
     4321 ggctaatccg tctaatttga taatcttcca cactctctga gtaacgcttt aagcaaagcg
     4381 ctaccaaagg cagtttctgt agattttttg caagagaggt tccgttagga gtttgcttta
     4441 acagccagct cgatgtcgct cttgggtgat caaggctact atctagacgc tttatgattc
     4501 ttaatagttg tcgtacagca attcgatctc tttggttcca atcaactcta ggggcgggtg
     4561 ccagacgctc ttgttgatgc tgttgattcc agtgaactag ccaatccctg tcatgtcggt
     4621 aaagccaagc gtatagcacc ccaccctcta aagactgtct tgccgcctta attccttggt
     4681 atttatgcac tagttgctgc cagtctttat gcttaacaga taaatcttca gagttaggtt
     4741 gcacagactg gctaacaggt cttgtcgtta tagagtgctc agtaagagca cttgcctgct
     4801 gtagcgcttc tataaccgtt agttttggca ataaggcttg ccacacaata ctatgctgta
     4861 aataactaaa ggcttttcta tgcttacgga atatactttt cagccaacac gtgtccttgt
     4921 tctctgccaa ctttaaatcc agtttctcta gtgcctcatc actaaaagtc tgcctcactc
     4981 tctccgccac caagtcatga cgaatgtgct tgcttttggt tagccctaga tcctgcgcta
     5041 agcgctgata aaacagcgtc cactgctcaa ggcttgggga aagctcttgc gctcgtggag
     5101 catctaacag aggggctata taagcagcta gtgctgttaa ttgagatagg gagtctttgg
     5161 ggtagtctga aagcagctca gtatgaccca aagcccaaaa ttgatgtcgg tgatcatcta
     5221 cagctctatc aaagaagact aaagcaccgt gttttggaca atatggcaaa gcgggcaaat
     5281 accaatctcg ttgccaaaag gcttccccat acctatttag ctgaagagca acgcaatcag
     5341 ggcagtatct aaagcggtta tcgctcttaa ctctagaagc agcgactcct agcattaaat
     5401 gcaccgcacc ttgcgcttgg tactccatta accgaatagc ttcgtctcgg cgctccttgc
     5461 ctacaaacgg agcatataaa gggaataagg tatgctcata aataagctgc tgaacagcgt
     5521 aacgtcctgt ttgatgtaga tgtcttgcta tcacacctaa atgcgagggc agacctaagg
     5581 tagcgaccac cttgcggttg ccatacacct catccaacag ctgcttagga ctaacaatcc
     5641 cttgataaac gcctgcccgt gcaatagtgc tataaatcag ctcattcgag tacggaacag
     5701 gaaagtttct catggctaac ctgcttgttt aaataagctc gccatatcca ctattacccc
     5761 tttccctttt aaccgttcat gcatggtttt ttcaggttgg cgttgtgaat agatataacg
     5821 taaatccgta tcaggcaagc tatcccagtc gctgggcttg actaccttta tagccgaaac
     5881 ctttttactc ttggagggct tttctagttc tgacactacc gtttcgcctt ccatcaacca
     5941 ctgcaaaaca agaggcagta acttttgtct tgtcatcgtt ggattctggc taaacgcttt
     6001 tttaatagtg ggaattaaca ggcttgaatc gtaatcctct ttcagcatca gatataaatg
     6061 acgctgatct tcggtatcta actcttgaag ggctttttct tctggtgttt gttcttgtat
     6121 cgctgcgata tctagctgaa gttggattaa ccgtttatca atctcgggaa cgactagatc
     6181 agaataacga gcaatgcgtt ctgggatacc cgagcgtaat gcctctagca tggggtgcac
     6241 aggctttaac tcatcttgat acacttgccg caataaacca gcggtaatac gctcattgcc
     6301 taaagctagc gcacggagct gagcgagtac aaaaagtttt actacaatgt ccatcactcc
     6361 ttggcttagc tcataccaca catcacggac ctcatccgat aacagcgcat ctttgcgttg
     6421 taaaagctgt aattgccaaa gattatccgt aaaagcgatc cactcttgat tgggctttcc
     6481 acgttgcgtt tgttgtatag gatcccagaa tatagctcca aaccctgccc ctctacgtgc
     6541 agaccgcaaa tcagcctcaa aaatctctcg tgctttaggg gtaccaatca acatcactgg
     6601 tacgccaata atattcacca tcgtcacaaa aaagttcagc atctcttgag atccacccga
     6661 acgagagcgg cttaaatgct gaatttcatc aataaccaac aaccctaaag catgtgcatt
     6721 ggctatttgc gacatcaaag ccaacatggt ttctatacca tgacgtttta agccataacg
     6781 acgctcatag ttcgagccca aggctcgatc caacgctctg aaaaaattca agcagatttc
     6841 ttttagcgaa ccattatgcg agcagtctat tttcaaatac accacctgct ctacattgag
     6901 ttcacgatgg taaatcacct gaggatacgt ggctagaata cgatgaagag aggtcgtctt
     6961 cccactacca gaacaaccaa ttaacaataa gctttgtgcc gtagatcgtg cctcctcaaa
     7021 gcgaaatgtc tccaactctc ccgtttgaac acgctcataa ccattttgta aatgcttttg
     7081 taaatctcct gttttaggat ttctgcctac gtagccacct cgaatcatga ccgaaatacg
     7141 ctcacttagt agcaaatgcg tacctaatgg ctgaaaatag tcatctggaa tacgacaaat
     7201 ggtatgagct ctgataacac gggacttttg caagtcagag gaagtaagct gtaaagagga
     7261 tttcagtgat gcagcactat tcactgactc ttgtaatggt ggtaaggcct cgataaaagg
     7321 attatcacga taagcctcta cccccgtatc acgataaact gcttgaatcc gggtagcact
     7381 catgactcat ccttttctgg tggatcctga aataattcag gcacgtatgt gggtaggctg
     7441 taatcttctt gatcatccgc ttccactgcg ttgaaaggaa taactttagc ctcatcacct
     7501 gaagagcttg gcttcaaatg ctccgcacgt tttttacgct ccgaggtcac ggcttctttt
     7561 ttattagttt taatctgctt aatgcgtgtt gatttgggct cagtagtact gggcgttaac
     7621 ttattcgctt tctgaatggt ttgctgaata aacgcctcaa gctccctgcg tttagttaac
     7681 tcatcctgct tcgcattggc tttattgtgt ttttcttgtg cttgtatatc ccaaacctcc
     7741 caaaatgaga gacctttaaa ctgccgacta cgttccgtca gattacagcg ccaaaataca
     7801 cggctgccaa cttgcggaaa caaataaatc gtatcaacca gcactgggtc ataagccgct
     7861 tctaaatgtt gaggtctagc tatatcagtg ctccgctgca accaaccctc acgcagaatc
     7921 tctgaccccg agtaatacaa accccacaaa ttaacgccaa atgaagaaat agagaccttt
     7981 cggcgaggca gtaacgctac tcgcaactgc tcttgctcca cagcccttaa actacctgta
     8041 cgatgctgca taccccattg ccatagctgg acaggaatag acggtaaatc tgtaggaaaa
     8101 tcagcatctc gatcgtattt atccatcacc agatggttat ttctgaataa gatcgtacgc
     8161 aaaataattt gtgtgaactc aaataccgac agagatgcat ctaacctata gtctgtttca
     8221 ccatggcttt tgatccgact gccctctaca atgccaggtg caaaggactt aaactcggct
     8281 tgtagtgttc taaaagtgct ttccactatg cctttagcat cgccacgtct aggtggagca
     8341 ctttccactc gcacattaaa actagaaact aaggcttcga cctgatgact cattaattcg
     8401 ccacggtccg ctagcaacac atctggcaaa cctacacacg gccagtctga gctactaatc
     8461 tcaatatcat gctgggcaca aatggccgtt ttgtcagagc aagcatttac aaaagcctgc
     8521 atcgccacca cataagacgg attttcaaag ccgatataaa agcccgtgat catccgacta
     8581 aacacatcaa tcacaatgta aagcgttggt cttcctatga ttttttggcg atcatgatga
     8641 tccactaaat aaatatcagc aatcgtggca tcaatctcat aacgactccc agggcctaac
     8701 gcctgagaag tggctgtact acttaagggt cgtacgtctt ttttatatac ccctgcttta
     8761 actctagact ttaagcgctg agctttaggg tattctcgat cataaaaata acgaaactga
     8821 cgtagtgttg ggtaatcctc ttggggaatg cgaggaaaat actgagcaaa caagtccaca
     8881 aatcgtctat aggcaacggt ggtctttgta cctttttgat ttaacaggtg cttttctatg
     8941 gtcaacctaa aaaggcgttc aatctcgggc gttaccttgg ttccttcacc cttaccatat
     9001 tctctggctc ggccaatctt tgctgttcct gtcgctgaac gtctttcccc tggtgcaccg
     9061 ctgtttttgt agtcaggaat taatgcatta ggcgtttgac cacgctgcca gtaacggcgt
     9121 aacaacttat aaactgtagc cttagtaacc ttatgttctt ggaccacatg ctcaacgagt
     9181 tcgcttctga ctttagggtc gaaacgatcc ttactattaa taataggaag aatttttcga
     9241 taatcctcct cgcgcttctg gaagctgaca gaatctacag acggctcttc taaatcaaga
     9301 tgtacataag ggtcatcagt tctaactaag cggccttcat ctaaatactg cattagcaac
     9361 aactcagccc tagcttgtgg aactccttta tcagcgctta tttgcatcca gacaacttgg
     9421 ccatcctcta tagccaaaat gcgatacgga tcattatcaa atagcacaac ctcattaatt
     9481 tgccacatag cgcaactcct ccatattcac tacttggcta atacagagat ctgcacactt
     9541 atttgccctg aaagacttat aaatattgaa cttaataaaa ccatttgcgg ttaaggctcg
     9601 tatctcactc aatgttttgc ctaactccaa atcataagca atatcaacct gcttacagac
     9661 attgataatg ttttcatctc ctttttcttg caggatatgg gccaatgggg atagttgtgc
     9721 taaaagctcc gcagaaactt cttctgtttt cactgaataa agccattcaa tattttcttt
     9781 tactacggga tttatttctt tatcagtaaa aatgaaccaa ggaatttgct tttgctgcca
     9841 atagcgacgc tctagttcta gtttttctaa ggtacgctcg tcttgtaagg ctgctgcagg
     9901 tttgacttga atagcaaact gctcaaaagg accatctttg cagtccacta aaaaatcagt
     9961 agacataacc tgatctacac cacgaataac aggatgctta ataccactat ctattgcaat
    10021 ctgcctggta tcactaggta ataaggggaa ctgctcgcgt atatctagca cgctgctctc
    10081 ccactcaaga ctgagaaaaa cagcaagctc taagtcagat agcaaatgat ggactcgtcc
    10141 cgtcttatga gaataaatac ggtgggaacg acctgaagaa ggaacttctt gtactgttag
    10201 ccatggaata tagtctttac catgcccttg gccacgcccc tctttaatac gacgggcaat
    10261 ttgcacttca gaaaatgaag agtttgcttt agccataaca aaagtccagt atgctttttc
    10321 acagcataac tggactgatt tcagtttaca actattctgt ctagtttaag actttattgt
    10381 catagtttag atctattttg ttcagtttaa gactttattg tccgcccaca agcccttttt
    10441 tatctatgat agtgattact caaccgtaac cgattttgcc aggttacgcg gctggtcaac
    10501 gtcggtgcct ttgatcagcg cgacatggta agccagcagc tgcagcggaa cggtgtagaa
    10561 gatcggtgca atcacctctt ccacatgcgg catctcgatg atgtgcatgt tatcgctact
    10621 tacaaaaccc gcatcctgat cggcgaagac atacaattgg ccgccacgcg cgcgaacttc
    10681 ttcaatgttg gatttcagtt tttccagcaa ttcgttgttc ggtgcaacga cgataaccgg
    10741 catatcggca tcaataagcg ccagcggacc gtgtttcagt tcaccagcag cgtaggcttc
    10801 agcgtgaatg taggagatct ctttcagctt caatgcgcct tccagtgcga ttgggtactg
    10861 atcgccacgg cccaggaaca gtgcgtgatg tttgtcagag aaatcttctg ccagagcttc
    10921 aatgcgtttg tcctgagata gcatctgctc aatacggctc ggcagcgcct gcagaccatg
    10981 cacaatgtca tgttcaatgg aggcatccag acctttcagg cgagacagct tcgccaccag
    11041 catcaacagc acagttaact gagtggtgaa tgctttagtg gacgccacgc cgatttccgt
    11101 acccgcgttg gtcatcagcg ccagatcgga ttcgcgcacc agagaagaac ccggaacgtt
    11161 acagattgcc agcgaaccaa ggtaacccag ctctttcgac agacgcaggc ccgccagggt
    11221 atccgcggtt tcgccagact gtgacaaggt gatcatcagg ctgttacgac gcacggcaga
    11281 tttacgatag cggaattcag aggcgatttc gacgtcgcac ggaatacctg ccagcgattc
    11341 aaaccagtag cgggaaacca taccggagtt ataagaagta ccacaggcga ggatctgaat
    11401 atgctcaacc ttcgacagca gttcgtcggc gttcggtccc agctcgctta aatcaacctg
    11461 accgtggctg atgcgcccgg taagggtgtt ttttatcgcg ttcggctgtt cgtagatctc
    11521 tttctgcatg tagtgacggt aaataccttt atcgcccgcg tcatattgca gattggattc
    11581 gatatcctga cgttttactt ccgcgccagt tttatcgaag atgtttaccg aacggcgagt
    11641 gatttccgca atatcgccct cttcaaggaa gataaagcga cgggtcaccg gcaacagcgc
    11701 cagctggtca gaagcgataa agttttcgcc catacccagg ccaatcacca gcggactacc
    11761 agaacgtgcc gccagcaggg tatccgggtg acgggagtcc atgatcactg taccgtacgc
    11821 accgcgcagc tgcgggatag cacgcagaac agcctcacgc agagtcccgc cttgtttcag
    11881 ctcccagttc accagatggg caatcacttc ggtgtcggtt tcagaaacga aggtatagcc
    11941 acgcgctttt aactcttcac gcagcggttc atggttttcg atgatgccgt tatgcaccac
    12001 cacaatgtgt tcagaaacat gcggatgcgc gttagcttct gaaggttcac cgtgggtcgc
    12061 ccagcgagtg tgagcaatac cggtgccgcc atccagagga tgttcttccg ctgcctgagc
    12121 cagcatctgg actttaccga ggcgacgcag gcgggtcata tgaccttccg catcaacaac
    12181 ggccagaccg gcagagtcat atccgcggta ttccagacga cgtaaacctt caagaaggat
    12241 ttctgctaca tcacgttgcg cgatcgcgcc aacaattcca cacatagttt ttgattccga
    12301 tttatatcgt tgtcggtcaa cctgtatgcc cgttttttcg ggcgccccga gccttgtaga
    12361 gagtggggtt atttttatag ttactgcttg tgggagggag attatgttat ctcctcatcc
    12421 cagatgaccg gattacccgg ccagaattac tttttcttta ccggacgacg ccagccttct
    12481 ttctgagtct gcggcacacg gctgatagct aatgcatttt cgccgacatt acgcgtcaca
    12541 gttgtacccg cagcaatggt cgcgcctttg cctactgtta ccggggccac cagctgagtg
    12601 tcggaaccga caaacacatc gtcgccgata atggtcttaa atttattcgc accatcgtag
    12661 ttgcaggtaa tggttcccgc gccgatgtta acgttatcgc caatttccgc atcgcccagg
    12721 taagtcagat gcccgacttt cgagccttta cccagacgcg cttttttcat ctcaacgaag
    12781 ttaccaacgt gtgcaccttc cagcaactca gcaccaggac gcaaacgagc aaacgggcca
    12841 atagtacagg ccgctgccag attcgcgtct tccacaacgg tatacggact gatttcgcaa
    12901 tcatcgccaa tcacgctgtt tttaatcacg caaccggtgc cgattttcac gcgatgaccg
    12961 agagtcacgt tgccctcgat gataacgtta gtatcaattt caacatcgcg cccgtgagta
    13021 agcgtaccgc gcagatcaaa acgcgccgga tcgcgcagca taacgcctgc taacagcagt
    13081 ttttcagcct gttcggactg gtaaacgcgc tccagacggg agagttgcag gcggttattc
    13141 acaccttcta cttcgcttaa acgttgcgga tgaacggcga cgatttcacg cccttcctga
    13201 tacgccagcg caataatgtc ggtgatgtag tattcgccct gagcattatt gttggtcagc
    13261 ttcgccagcc agcgtttcat atctgcgccg ttagcgatca gaatgccggt gttgatctcc
    13321 tgaatctgac gctgctcgtc ggtggcatct ttgtgctcaa caatgccggt aactttgcca
    13381 ttttcacggg tgatacgtcc ataaccggtc ggatcatcca gtttcaccgt cagcagacca
    13441 atgccaccct gcggtttagc atcacgcagg cgctggagtg tttcgacaga gatcagcggc
    13501 acgtcgccgt agagcattaa aatgtcttca tcatcggcaa agaaaggtgc ggcctgctgc
    13561 attgcatgac ccgtacccag ctgctctgcc tgaagcaccc agttaaggtt gtcgtctttc
    13621 agcgcctgtt taagcagatc gccgccgtga ccgtacacca agtgaacgtg cgctgcgcct
    13681 aattcattcg cagcatcaat gacatgctga accatcgctt tcccggcaag ggtatgcagc
    13741 actttcggaa gatcggaata catgcgcgtg cctttgcctg cggcaaggat cactacgctc
    13801 atagcattat tcaacatacg cgtcctgact gtaatttgag aacgaattta aaccgcttca
    13861 ccttgaaaaa actacatttt tttcatcgtg aaatggacag aggataaatt gttcaataac
    13921 ggattatccc cgctgacgaa agtgccattt tcgaccatcg tcacgccttt tgtctgcaga
    13981 aaattaaggc agtaccttca tttaagcgtc aggtggatgt ttttgctctt atttcgatca
    14041 atgaataaac gggaaaacag gggtgtttta tatcagcagg atctatgtga acgctcttca
    14101 ggacggatca cgcgtaaaaa aaagccagcc tgtttccaga ctggcttttg tgcttttcaa
    14161 gccggtgtta catcgctttt ttggtcaact cgataacgcg cagctgcgcg atcgctttgg
    14221 ccagttccgc agacgcctga gcgtaatcta cgtcgccgtg agagctgcta atgtgctctt
    14281 cagccttacg tttcgcttcc atggctcgcg cttcgtcgag atcctggccg cgaattgcgg
    14341 tgtcggccag aacggtcacg ttgccaggct gcacttcaag aatgccgcca gacagataga
    14401 taaactcttc gtgaccgtgc tgtttcacga tgcgaatcat accaggctta atggcggtga
    14461 gcagcggtgc gtggccaggg tagatcccca gttcaccttc gctacccgtt acctggattt
    14521 tctcgaccag accagagaac atttgttgct ctgcgctgac gacgtccagg tggtaagtca
    14581 ttgccatatc accctccgat taaggcgtta aagttttttg gctttttcca cagcttcttc
    14641 gatggaaccg accatgtaga acgcctgctc cggcaggtga tcgtattcgc cttccatgat
    14701 gcctttaaag ccacggatgg tgtctttcag ggagacgtat ttacccggag aaccggtgaa
    14761 tacttctgcc acgaagaacg gctgggacag gaagcgctgg atcttacgag cacgcgctac
    14821 caccagtttg tcttcttcag acagttcatc catacccaag atggcgatga tgtctttcag
    14881 ttcctgataa cgttgcagga tggactgaac gccacgcgcg gtgtcgtagt gttcctgacc
    14941 aaccaccagc gggtccagct gacggctggt ggagtccagc gggtcaacgg ccgggtagat
    15001 acccagagac gcgatctgac ggctcagtac cacggttgcg tcaaggtgcg caaaggtggt
    15061 tgccggagac gggtcagtca agtcatccgc aggtacgtat accgcctgta cggaggtgat
    15121 agaaccagtt ttggtggagg tgatacgttc ctgcagaacg cccatctctt ccgccagggt
    15181 cggctgataa cctaccgctg aaggcatacg gcccagcagt gcggatactt ccgtaccggc
    15241 cagggtgtaa cgatagatgt tgtcaacgaa cagcagaacg tcacgacctt cgtcacggaa
    15301 tttctcagcc atggtcagac cggtcagagc aacgcgcaga cggtttcccg gcggctcgtt
    15361 catctggcca tacaccaggg atactttgtc gataacgttg gagtcggtca tttcgtggta
    15421 gaagtcgtta ccctcacgag tacgttcacc tacgcccgca aacacagagt aaccggagtg
    15481 ctcgatcgcg atgttacgaa tgagctccat catgtttacg gttttaccta cacccgcacc
    15541 accgaacaga ccaactttac cgcccttagc gaacggacac atcaggtcga taactttgat
    15601 accggtttcc agcagttcct gagagtttga cagctcttcg taggaaggtg ctgcgcggtg
    15661 aatcgcccaa cgctcttctt caccgatctc gcctttcatg tcgaccggtt cacccagtac
    15721 gttcatgata cggcccagag tcgctttacc taccgggact tcgatcgggt gttcgaggtc
    15781 ttttacatcc agaccgcgac gcagaccgtc ggaggaaccc attgcgatgg tacgcacgat
    15841 accgccgccg agctgctgct gaacttccag caccagacgc tcattaccat tttgcacctc
    15901 aagagcatcg tacacgcgcg gtacggcatc ctgagggaat tcgacgtcaa ctacggcgcc
    15961 gattacctgg acaatctttc cagtagccat cttaaatcct ctacgaaata acctgtttaa
    16021 accgcggcgg cccccgagac gatctcggtg agttcctgag taatgctggc ctgacgagct
    16081 ttgttgtata ccaactgcag ctctttaatc aggctgccgc cattgtcggt cgcggctttc
    16141 atcgccacca tacgggcggc ctgctcgctg gccaggtttt caaccacgcc ctgataaacc
    16201 tgagattcga cataacgacg cagcagggta tccagcaacg ccttcggatc gggttcgtac
    16261 aggtaatccc aggatttatg tttcagatca tcatcatctg atgccggtaa cggcagcagc
    16321 tggctgatgg tcggaacctg agacatggtg ttaataaatt tgttgctgac aatgtaaagc
    16381 ttgtccagac ggccttcgtc gtaagcctgc aacatcactt ttaccggacc gatcagttcg
    16441 gacagggaag ggttatcccc catgccggtg acctgggcaa caacattgcc gcccacggag
    16501 ttgaagaacg acacgccttt cgagccgatc attgcgaggt cgcattgaac gcttttgtcg
    16561 gtccaggtct tcatttccgc cagtagtttt ttgaacaggt taatgttcaa accaccgcac
    16621 aaaccacggt cggtcgacac caccaggtag cccacgcgtt taacgtcgcg gtcttccagg
    16681 taagggtgct tatattccag attaccgtgt gcaaggtgac caatcacttt gcgcatggtt
    16741 tctgcataag gacggctggc cgccatgcga tcctgcgatt tacgcatttt ggaagcggcg
    16801 accatctcca tcgctttagt gatcttttgc gtgttctgga cgcttgcgat cttactacgt
    16861 atctcttttg cgccggccat gagcttctcc tcaatgcctt gcggcctgcc ctaaggcaag
    16921 ccgccagacg ttaccaggat tgggttgctt tgaaggaatc gaggatgcct ttcagcttgc
    16981 cttcgatttc gtcgttgtag ccaccggtct ggttgatctc ttgcatcaac ggagcgtgat
    17041 cacggtcgac gtaagccagc agagcggctt cgaagctgcc aattttcgac agttcaacat
    17101 ccgccaggta accacgttct gctgcgaaca gaaccagaga ctgctgcgca acggacatcg
    17161 gcgcatactg tttctgtttc agcagttcgg tcactttctg accgtggtca agctgcttac
    17221 gtgttgcatc gtcaaggtcg gatgcaaact gagagaacgc tgccagttca cgatactgtg
    17281 ccagagcggt acggatacca ccggacagtt ttttcatgat cttggtctgt gctgcaccac
    17341 caacacggga tacggaaata cccgggttaa ccgcaggacg aataccggcg ttgaacaggt
    17401 tggtttccag gaagatctga ccatcggtaa tggagattac gttggtcgga acgaacgcag
    17461 aaacgtcacc cgcctgagtt tcgataatcg gcagtgcggt cagagaaccg gttttccctt
    17521 tcacttcacc tttggtgaag gcttcaacgt attcggcgtt aacacgtgca gcacgctcca
    17581 gcagacgaga gtggaggtag aaaacgtcgc ccgggaatgc ttcacgtcct ggcggacgac
    17641 ggagcagcag ggagatctga cggtaagcaa cagcctgttt agacaggtca tcgtaaatga
    17701 tcagcgcatc ttcaccgcgg tcacggaagt attcgcccat tgcgcaaccg gcatacggtg
    17761 ccaggtattg cagtgcagcg gattcagacg cggttgctac cacaacgatg gtgttagcca
    17821 gtgcgccgtg ctcttccagt ttacgtacca cgttagaaat ggtggacgct ttctggccga
    17881 tagcgacata gatacatttg ataccggaat cgcgctggtt gatgatggca tcgatagcca
    17941 gtgcggtttt acctgtctga cggtcaccga tgatcaattc acgctgacca cgaccgattg
    18001 ggatcatgga gtcaacggct ttataaccgg tctgtaccgg ctgatctacg gactgacgtt
    18061 cgataacgcc cggagcgatt gcttctacag cagagaagcc gtcgtgatcc agcggacctt
    18121 taccgtcgat tggtgcaccc agagtgttaa ccacacggcc cagcaggcca cggccaaccg
    18181 gaacttccag gatacggcca gtacacttaa ctttcatgcc ttcggcaagg tcagcgtacg
    18241 gacccataac aaccgcacct acagagtcgc gctcaaggtt cagtgcgata gcgtaacggt
    18301 tacccggcag ggagatcatt tcaccctgca tacaatcggc caggccgtga atgcggataa
    18361 caccgtcact tacagaaaca atagtacctt cgttgtgagc ttcactcaca acattgaact
    18421 gagcaatgcg ctgcttgatc agttcgctga tttcggtgga attcagttgc atgctccagt
    18481 ccccttaaga ctgcaagacg tctgcaaggc gctcaagacg accgcgtacg ctgccatcaa
    18541 tgaccatatc acccgctcgg atgataacgc ctgccattac agacttatcg attttgcaat
    18601 tcagcttaac tttgcgtgac agacgttttt ccatcgcagc agaaattttc gcgagctgtt
    18661 gttcactcag tgcggcagcg gaaatgacgt ctacctcagc ggtagcctca ctcacggcac
    18721 gaaggtgaat aaactgctcc agaacatccg ggagcgcgtt aagacgacca ttttcagcca
    18781 ttacccgaat caggttctga ccgttttcgt ccagttgctc accacaaact gcgataaacg
    18841 actcggcgag cgtttctggc gcaagcgcgc cagagagaag ctctgccatt tgttcgtttt
    18901 tggttacctc ggcggcaaac gccagcatgt cctgccagcg ttctacactt tggtgttcga
    18961 cggcaaagtc aaaagctgct ttggcgtagg ggcgagctac cgtaataaat tcagacatca
    19021 gcccctccct ccttacagtt cagcgacaag tttatccacg atgtcgctgt tagcagcttc
    19081 atccacggaa cgttcgatga tcttctcggc gccagcaaca gccaggatag caacttgctt
    19141 acgcagctct tcacgggcac gtttacgctc ggcttcaatt tctgcctgcg cctgggccac
    19201 gattttagta cgttcctgtt ctgcctcagc tttcgcttcg tccagaatct gcgagcggcg
    19261 tttgttcgcc tgctcgatga ttacctgggc ttccgctttc gcttttttca gctggtcggt
    19321 cgcgctggcc tttgcaaggt caaggtcctt atgtgctcgt tctgcggaag caaggccgtc
    19381 agcaatttct ttttgacgtt tttcgatggc tgccattaat ggcggccata cgtacttcat
    19441 gcagaacaga acgaacagga caaacgcgat ggcctggccg aggattgttg cgttaagatt
    19501 cacagcacaa tgcctctatt tagttaacgt tctgatattg ctctttaaat aaaagcaacg
    19561 cttactacgc gacagcgaac atcacgtaca gacccagacc tacagcgatc atcgggatag
    19621 catccaccag acccataacg ataaagaact gagtacgcag cagaggaatc agatcaggtt
    19681 gacgcgctgc gccttccagg aatttacccc cgaggatgcc gataccgatc gcagcaccga
    19741 ttgccgccag acccatcatc acagcggcag ccatgtacag cagatccata ttcaggtttt
    19801 ccatgacagt ctccagtttg tttcagttaa aacgtagtag tgttggtaaa ttaatgttct
    19861 tcagacgcca tcgacagata gacgatcgtc agaaccatga agatgaaggc ttgcagcgta
    19921 atgatcagga tgtggaaaat ggcccacggc acattcagga tccactgtga ccaccacggc
    19981 aacagaccag caatcagaat gaaaatcagc tcaccggcat acatgttacc gaacagtcgc
    20041 aaaccgagtg aaactggttt ggacagcagg cttacccctt caaggattaa gttgacagga
    20101 atgaacgccc agtgattgaa cggctgcagc gtcaactctt tcgtgaagcc gccgatgcct
    20161 ttcattttga tgctgtagaa cagaatcagg ataaatacgc ccagtgccat agacagcgtt
    20221 acgttcacgt ccgcagacgg aaccacacgc agtgcaggca gacccagtac atgttcagca
    20281 atgtacggca gcaggtcgat aggcagtaaa tccatcaggt tcatcaggaa tacccagacg
    20341 aagatcgtca gggccagcgg agcaatcagc ttgcttttgc catggtacat gtctttcacg
    20401 ctaccattaa caaagccgat caccagctca atcgcggtct gaaacttacc tggcacaccg
    20461 ctggtcgcct ttttggctac gctacggaat aaaaccagga acaacagacc cagcaccacc
    20521 gagaagaaca tggagtcaat attgattgtc cagaaggtgg ctggggggtt ttgtggatcc
    20581 accagcgaga atgtacgcag gtccagctga aggttattca ggtggtgtcc tatgtaatcc
    20641 tgcggcgtca tattttctga agccatgatg ccttttaccc tttgttgtta attacagccg
    20701 gtgccagtat ctgaaccacc agcaccaaaa cccacgtaac gatcagcggc aagaataccg
    20761 cctttaaaac cgccaacgcc accaccagta acaccaacat cgccagaact ttgaaagctt
    20821 cgccaaatgc gaatgtccag gccacccggc ctttcgctgg tgtatacgcc tggtgacgcc
    20881 aggcaaatat cataaacaaa acgttaggca gaaagactgc caggcccccg cttattgcag
    20941 agacgcccca gaaggggtct ttgaggctga acagcaatcc acttgctatc accaccagta
    21001 actgaacgag cagaagcttc cgagcaacgt ttcgactcac gagcgacaca gacatcacgt
    21061 ttttcactcc tgctcccttc gaggtatgcc gcgtgtcgta taaaactttc tttaaggctt
    21121 agagtcaagc atcaaaaagc ggtcaaatta tacggtgcgc ccccgtgatt tcaaacaata
    21181 agtagccaaa aggtgaataa atgtttaaat atttttccag tgcatacaat tgcgactttt
    21241 ccgctaaccc tgttcgatca tgaaaaactg taaataacgc gtaaacacta gtgataaagc
    21301 gtgcttcaga tcacatattg cgcatgttcg cgcacagtat atttatttac ttggcaaatg
    21361 atgcctttgc aagtttatga tatttcagtc taaaaacaga tactgtttta ataaatgaca
    21421 tttacacaac aaaaaccacc cattgacatt tttaataatg tttta
//
LOCUS       DAZOYL010000037        21424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZOYL010000037 DAZOYL010000000
VERSION     DAZOYL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.037"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            241..525
                     /gene="pduA"
                     /locus_tag="ACHUOY_001038"
     CDS             241..525
                     /gene="pduA"
                     /locus_tag="ACHUOY_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR0681881.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            522..1334
                     /gene="pduB"
                     /locus_tag="ACHUOY_001039"
     CDS             522..1334
                     /gene="pduB"
                     /locus_tag="ACHUOY_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFR0681882.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            1353..3017
                     /gene="pduC"
                     /locus_tag="ACHUOY_001040"
     CDS             1353..3017
                     /gene="pduC"
                     /locus_tag="ACHUOY_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR0681883.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            3027..3695
                     /locus_tag="ACHUOY_001041"
     CDS             3027..3695
                     /locus_tag="ACHUOY_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR0681884.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3710..4228
                     /gene="pduE"
                     /locus_tag="ACHUOY_001042"
     CDS             3710..4228
                     /gene="pduE"
                     /locus_tag="ACHUOY_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR0681885.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4238..6070
                     /locus_tag="ACHUOY_001043"
     CDS             4238..6070
                     /locus_tag="ACHUOY_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR0681886.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            6060..6410
                     /locus_tag="ACHUOY_001044"
     CDS             6060..6410
                     /locus_tag="ACHUOY_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR0681887.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            6430..6705
                     /gene="pduJ"
                     /locus_tag="ACHUOY_001045"
     CDS             6430..6705
                     /gene="pduJ"
                     /locus_tag="ACHUOY_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR0681888.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6729..7151
                     /locus_tag="ACHUOY_001046"
     CDS             6729..7151
                     /locus_tag="ACHUOY_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR0681889.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            7154..7786
                     /locus_tag="ACHUOY_001047"
     CDS             7154..7786
                     /locus_tag="ACHUOY_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR0681890.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7783..8274
                     /gene="pduM"
                     /locus_tag="ACHUOY_001048"
     CDS             7783..8274
                     /gene="pduM"
                     /locus_tag="ACHUOY_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR0681891.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8278..8553
                     /gene="pduN"
                     /locus_tag="ACHUOY_001049"
     CDS             8278..8553
                     /gene="pduN"
                     /locus_tag="ACHUOY_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR0681892.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8565..9572
                     /gene="pduO"
                     /locus_tag="ACHUOY_001050"
     CDS             8565..9572
                     /gene="pduO"
                     /locus_tag="ACHUOY_001050"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR0681893.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9569..10954
                     /gene="pduP"
                     /locus_tag="ACHUOY_001051"
                     /pseudo
     CDS             9569..10954
                     /gene="pduP"
                     /locus_tag="ACHUOY_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            10965..12077
                     /locus_tag="ACHUOY_001052"
     CDS             10965..12077
                     /locus_tag="ACHUOY_001052"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR0681894.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            12074..13419
                     /locus_tag="ACHUOY_001053"
                     /pseudo
     CDS             12074..13419
                     /locus_tag="ACHUOY_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            13422..13976
                     /gene="pduT"
                     /locus_tag="ACHUOY_001054"
     CDS             13422..13976
                     /gene="pduT"
                     /locus_tag="ACHUOY_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR0681895.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13976..14326
                     /gene="pduU"
                     /locus_tag="ACHUOY_001055"
     CDS             13976..14326
                     /gene="pduU"
                     /locus_tag="ACHUOY_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR0681896.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14331..14774
                     /locus_tag="ACHUOY_001056"
     CDS             14331..14774
                     /locus_tag="ACHUOY_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR0681897.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(14875..15204)
                     /locus_tag="ACHUOY_001057"
     CDS             complement(14875..15204)
                     /locus_tag="ACHUOY_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR0681898.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15376..16434)
                     /locus_tag="ACHUOY_001058"
     CDS             complement(15376..16434)
                     /locus_tag="ACHUOY_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0681899.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16632..17105)
                     /gene="sbmC"
                     /locus_tag="ACHUOY_001059"
     CDS             complement(16632..17105)
                     /gene="sbmC"
                     /locus_tag="ACHUOY_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR0681900.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17224..18390)
                     /gene="dacD"
                     /locus_tag="ACHUOY_001060"
     CDS             complement(17224..18390)
                     /gene="dacD"
                     /locus_tag="ACHUOY_001060"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR0681901.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18599..20026
                     /gene="sbcB"
                     /locus_tag="ACHUOY_001061"
     CDS             18599..20026
                     /gene="sbcB"
                     /locus_tag="ACHUOY_001061"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR0681902.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(20069..20296)
                     /gene="tsuB"
                     /locus_tag="ACHUOY_001062"
     CDS             complement(20069..20296)
                     /gene="tsuB"
                     /locus_tag="ACHUOY_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR0681903.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20310..21368)
                     /gene="tsuA"
                     /locus_tag="ACHUOY_001063"
     CDS             complement(20310..21368)
                     /gene="tsuA"
                     /locus_tag="ACHUOY_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR0681904.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
BASE COUNT         5543 a         5586 c         5287 g         5008 t
ORIGIN      
        1 ttccttcttc gaactaaact caaccaaata ttactcgcga ttaaactcac tagccaacgc
       61 aattaattca cattgaaata tccacaaaac aaccaataaa ttaacaacat atagttcata
      121 aaagcaattt tataatattt gttctctatt aatttctctc attagccgat taagtcagcc
      181 attctttttc ctcgcttttt tttatagtca tatcgtcaaa aaaatccagg cgaggtcttt
      241 atgcaacaag aagcgttagg aatggtagaa accaaaggct taactgccgc catagaggcc
      301 gcagacgcca tggtgaagtc ggcaaatgtt ttgctggtcg gctacgaaaa aataggttcc
      361 gggctggtga ccgtcatcgt tcgtggtgat gtcggggcgg taaaagcggc aacagacgcg
      421 ggtgccgcag ctgcgcataa cgtcggcgaa gtaaaagccg tacacgttat tccccgccca
      481 cataccgatg tcgaaaaaat cttaccaaag ggaattaacc catgagcagc aatgaactgg
      541 tcgaacagat catggcgcag gtcatcgctc gcgtagcgac gccagagcat gaggttctcc
      601 cgaaaaccac tcatcaaata cgagagacgg ctatggcaga aaaaagctgc acgctaacag
      661 aatttgttgg caccgctatt ggcgacactg taggtctggt cattgcgaac gttgatagcg
      721 ccctgttgga cgcgatgaaa cttgaaaagc gctatcgttc cattggcatt ctgggtgcgc
      781 gtattggcgc agggccacac attatggccg ctgatgaagc ggtaaaagcc accaacactg
      841 aagttattag cattgaactt cctcgcgata ctaaaggcgg tgccggacac ggctcactga
      901 tcctcttagg tggcaacgat gtttccgacg tgaaacgcgg tattgaagtc gccctgaaag
      961 aactcgatcg gacctttggc gatgtctatg ccaacgaagc cggacatctt gaactgcaat
     1021 ataccgcccg cgccagctat gcactggaaa aagcgtttgg tgcaccactg ggacgggctt
     1081 gcggcgttat tgtcggcgct ccggctgctg tgggtgttct gatggccgac accgcgctga
     1141 agtccgccaa tgtggatgtt gtggcataca gctccccagc tcaagacacc agctacagca
     1201 atgaagtcat tctgattatc tcgggtgact ccggcgcagt ccgccaggcg gtcatctccg
     1261 cacgcgaaat cggtaaaacc gtgctttcga ctctcggcga tacaccgaaa aacgatcacc
     1321 cttcctacat ctgaattctc tgaggcagat ttatgagatc gaaaagattt gaagcactgg
     1381 caaaacgccc ggtcaaccag gacggtttcg ttaaagagtg gattgaagaa ggctttattg
     1441 cgatggaaag cccgaacgat cctaaaccgt caatcaaaat tgttaacggc gtcgttaccg
     1501 aactggatgg aaaacccgcc agccagtttg acctgatcga ccactttatc gcccgctacg
     1561 gcatcaatct cgcgcgcgcc gaagaagtca tcgcgatgga ttcggtaaaa cttgccaata
     1621 tgttgtgcga tccaaatgtc aaacgtagcg atatcgttcc ccttaccaca gcaatgacgc
     1681 ctgcaaaaat cgtcgaagtg gtttcgcaga tgaacgtggt agagatgatg atggcgatgc
     1741 agaaaatgcg cgcccgtcga acaccatcac aacaggctca cgtcaccaac gtcaaagata
     1801 acccagtaca aattgccgcc gacgcagctg aaggcgcatg gcgcgggttt gatgaacagg
     1861 aaaccaccgt tgccgttgct cgctatgcac cgttcaacgc catcgccctg ctggtcggct
     1921 cacaggtggg acgtccgggc gtcttgaccc agtgttcact ggaagaagcc accgaactga
     1981 aactcggtat gttaggccac acctgttacg ccgaaacaat ttctgtctat ggtacagaac
     2041 ctgttttcac caatggtgat gataccccgt ggtcaaaagg gtttctcgcg tcatcctatg
     2101 cttctcgtgg cttaaaaatg cgctttactt ccggctccgg ctctgaagta cagatgggtt
     2161 acgcggaagg caaatcaatg ctttacctcg aggctcgttg catttacatc accaaagccg
     2221 caggcgttca gggtctgcag aatggttcgg taagctgcat cggtgttcca tcagctgtgc
     2281 catcaggcat ccgcgcagta ttagcggaaa acttaatttg ctcatcgctg gatctggaat
     2341 gcgcctccag taatgaccag acctttaccc actcggatat gcgccgtacc gcacgtttcc
     2401 tgatgcaatt tctgccaggc accgacttta tctcttccgg tttttccgct gtgccgaact
     2461 acgacaacat gtttgctggt tcaaacgaag atgctgaaga cttcgatgat tacaacgtca
     2521 ttcagcgtga cctgaaggtt gatggtgggc tgcgaccagt acgcgaagaa gatgttatcg
     2581 ctatccgcaa taaagccgct cgcgctttac aggctgtctt tgccggtatg ggactaccgc
     2641 ctattaccga tgaagaagtc gaagcggcaa cctatgccca cggttcaaaa gatatgcctg
     2701 agcgcaatat tgtggaagac atcaagttcg cccaggagat tatcaataaa aaccggaaca
     2761 gcctggaagt ggtgaaagcc ttggcgcaag gcagttttac cgatgtcgcc caggacatgc
     2821 tcaacatgca aaaagcaaaa ctgaccggcg attatctcca tacctcagcc atcatcgtcg
     2881 atgacggaca agtgctctct gcggtcaatg acgtcaatga ttacgccgga ccggctacag
     2941 gttaccgcct gcaaggggaa cgctgggaag agatcaaaaa tattcccggt gcacttgatc
     3001 ccaacgaaat tgactaaggg tgagaaatgg aaattaacga aaaattactg cgtcagatta
     3061 tcgaagacgt gctggcagaa atgcaaccca gcgacaagtc cgtttcattt cgcgcacctg
     3121 tctctgcaac tgtcccttcc gcgccagata ccggaaattt tctgaccgaa attggcgaag
     3181 cccaacaagg cactcagcaa gatgaagtca ttattgctgt aggcccggcg ttcggcctgg
     3241 cgcaaaccgt caatatcatc ggtattccgc ataaaaacat cttgcgcgaa gtgattgccg
     3301 gtatcgaaga agaaggcatt aaagcccgtg taattcgctg ctttaaatcg tccgatgtcg
     3361 cattcgtagc agtcgaaggc gatcgtctta gtggttcagg tattgctatc ggtattcagt
     3421 cgaaaggcac aacggttatc caccagcagg gacttcctcc cttgtctaac ctggaacttt
     3481 tcccacaagc gcctttgttg acgctggata cttatcgtca gattggcaaa aacgccgcgc
     3541 gttatgcgaa gcgcgagtct ccacaaccgg tgccgaccct caacgatcaa atggcgcggc
     3601 cgaaatacca ggccaaatct gccattttgc atattaaaga gacgaagtac gtggtcactg
     3661 gcaaaaaacc gcaagaactg cgcgtgacgt tttaacaaag gaaaccttca tgaataccga
     3721 tgcaattgag tcgatggtca gggacgtgtt gaaccgcatg aacagtttac aggatgcagc
     3781 tcctgtttct gcggtcccca acgcatcaat actgagcgcc aaagtgacgg attacccgct
     3841 ggcaaataaa catccggaat gggttaaaac agcgaccaat aaaacgctcg acgatttcac
     3901 tctggaaaat gtgctaagcg ataacgtcac cgccctggat atgcgcatta cccctgaaac
     3961 attgcgtatt caggctgcga ttgccagaga tgctggtcgc gatcgtctgg cgatgaattt
     4021 tgaacgcgcc gctgagctta cctcagtacc agatgatcgc atcctcgaaa tctacaatgc
     4081 gttacgccct tatcgttcaa ctaagcaaga acttattgcc attgctgacg atcttgaaca
     4141 gcgttatcag gccaaaattt gtgctgcatt tgtccgtgaa gctgcggaac tttatgtcga
     4201 gcgcaaaaaa ctgaaaggcg acgattaagg acgaactatg cgatatatag caggcattga
     4261 tatcggcaac tcatcaacag aggttgcgct ggcaacgctc agtgcaactg gagagttgtc
     4321 gttcgtgagt agcgccctgg aggaaaccac cggaatcaaa ggtacgttgc gtaacgtcca
     4381 tggtattcag gaggcgctcg cacaggcaac gaaaaaagtc ggcatcaatg ttagcgatat
     4441 ttcgcttatt cgtatcaacg aagcaactcc ggtcattggt gatgtggcga tggaaaccat
     4501 cactgaaact atcatcaccg aatcaaccat gatcggtcac aacccgaaaa ctccgggcgg
     4561 agtggggctg ggcgtagggt tcaccatcac tccgcaagag ttattaacac gcccggcgga
     4621 cacaccttac atcctggtgg tgtcatcggc atttgatttt gccgatatcg cgacgatgat
     4681 aaacgcctcc gtacgcgccg gatatcagct gactggcgtt attctgcaac gcgacgatgg
     4741 cgtactggtc aataaccgcc tggaaattcc tttacctatt gtggatgaag tcctctacat
     4801 cgatcgtatt ccgctcggca tgctggcagc aattgaagtt gccgttcccg ggaaagttat
     4861 cgaaaccctg tctaacccgt atgggattgc caccgtattc gccctcaatg ctgaagagac
     4921 taaaaatatt gttcctgtcg cgcgggcgct aatcggcaac cgttcagcag tggtagtgaa
     4981 aacgccctct ggcgacgtta aagcccgttc gatacccgcc ggaaatatcg aactgctttc
     5041 tgccgggcgc acaacgcgcg ttgatgtagc tgctggtgcc gatgccatta tgaaagcggt
     5101 cggtgagtgc ccaaaactgg aaaacgtgac gggagaacca ggcaccaaca tcggcggaat
     5161 gctggaacat gttcgccaga ccatggcgga actgaccaac aaacccagca acgaaatttt
     5221 tattcaggat ttgctggcga tagatacctc tgtccccgtc agcgtaaccg gtggtctggc
     5281 aggtgaattt tcactggaac aggctgtggg tattgcctct atggtgaaat ccgaccgtct
     5341 gcaaatggcg atgatcgcca gtgaaattaa gcaaaaactg catgttgatg ttcaggtcgg
     5401 aggcgcagaa gcagaagccg ccattcaggg ggccttgact acccctggaa ccacacgtcc
     5461 actggcaatt ctggacttag gtgcaggctc taccgatgcc tccatcatca atcagagtgg
     5521 ggaaatcgtt gccactcacc ttgcgggagc gggcgacatg gtaacgatga ttatcgctcg
     5581 tgagctgggc ttaaatgatc gttatctggc agaagaaatc aaaaagtacc cgttagcaaa
     5641 agtagaaagc ctgttccact tacgccatga agacggtagc gttcagttct tccctactcc
     5701 cctatccccc catgtatttg cccgcgtctg tgtagtgaaa cccgacgaac tggtgcctat
     5761 tcccggtgat ttgacgctgg aaaaagtacg cgctgttcgt cgcagtgcga aagagcgtgt
     5821 cttcgtaacc aatgccctgc gcgcgctacg tcaggtaagc cctgccggaa atattcgcga
     5881 cattccgttc gtcgttctgg tgggtggttc ttcgctggat ttcgaagtac cgcaattggt
     5941 caccgacgcg ctggcacatt atcgactggt cgccgggcgg gggaatatcc gtggtagcga
     6001 aggtccgcgc aacgcggtag ctactgggct actcatcgcc tggcacaagg agtcgattca
     6061 tggaaagtaa agagcgtgca ccagccatcg ttgtcatgga gatcggtgac tgtatcacta
     6121 catgggatga ggtgcttctt ggcattgaag aggaaggtat tccctttcgc attcaacaca
     6181 ttccgtcagg tgaagttatc gatagcgcct ggctggctgc tcgtcaatct cccctgttgg
     6241 ttggcattgc ctgcgaccgg gaagaactga ttgtgcatta caaaaactta ccggcatcag
     6301 cgccactgtt tacgctgacg tatcaacaag ataaccatac ccggcgcagc atcggcacta
     6361 acgctgcccg cctggtaaaa ggaattccct tccgggaatt ccactcataa tccacaggag
     6421 aaacgctgta tgaacaacgc actaggactg gttgaaacaa aagggttagt tggcgcaatt
     6481 gaagctgcag atgccatggt taaatccgcc aacgtgcagc tggttggcta tgaaaagatt
     6541 ggttcgggtc tgattacggt gatggtccgc ggtgatgttg gcgcagtaaa ggccgccgtt
     6601 gatgcgggga gcgctgcggc aagcgccgtt ggcgaagtga aatcctgcca tgtcatcccg
     6661 cgcccacaca gtgatgttga ggctatttta cctaaatcgg cataacccca tttgctaagg
     6721 agcacagcgt gaagcaatca ctgggattac ttgaagccag tggtctggct ctcgccatca
     6781 gttgcgcaga cgctatggca aaagccgcgt ccatcacgct ggttggcctt gagaagacca
     6841 atggctcagg ctggacgctc atcaaaatca ccggtgacgt cgcctcggtt aatgcggcga
     6901 tcgcgacggg agccagtttc gccggacagc gaaatggcct tgtcgcacac aaagtgattg
     6961 cccgcccggg agacggcatt ctgccccaca caactgaagc attgaccgtg acggttgaaa
     7021 caagcaaaac accagcaaca accgtcacct cagagattat cagttgtaat ttgtgtcttg
     7081 acccggcatg tcagcggcaa aaaggtgagc cccgctcact ttgcctgtac gccaataagc
     7141 gaggggagta gcagtggata aacaaacctt gcaggaaaca gtgagccaga ttctggatga
     7201 gatgcgcgag cgccctatcc cactgggcgt ttccagtcgc catatacatc tttcatcagc
     7261 ggattatttg cgtctttttc ctgaacaacc gattagcgag aaaaagccgt taatgcaacc
     7321 tggccaatac gctgctgaac aggttgttac gctggaggga ccaaaaggca aattgcacaa
     7381 cgttcgcttg ttggggccgc tacgcaatgc aagccaggtg gaaatttccc ggacggacgc
     7441 cagaacgtta gggatcaccg ccccgctacg catgtcaggc gatctcagcg gcactccggg
     7501 gattcgtttg ataagcccat ttgcggaact ggagatcaca tctggcgtca ttgtcgcgca
     7561 acggcatatt catatgtctc cgctggatgc cctgatttat ggcgttacgc atggcgataa
     7621 agtggcggtt gccattgaag gtgatgagcg acgactgata ttcgaaaacg tcgctgttcg
     7681 cgtctctccg gatatgcgtc ttgaaatgca tattgatacc gatgaggcca acgccgcagg
     7741 cgctgatagc cctgacgttt ttgccacact ggtgtcagtg ccatgaataa catgctgatg
     7801 caacgtattg ttgacgaagt ggtttttcgc cttaaacaac gtgcgggaaa aacactcgtg
     7861 ctgactgttt ttcaattgcg agacgccagc gttcaagaga gcgttcatca gtatgcgtca
     7921 ttacaaatcc gatatgtcga tctgccgctg ctgcgacaac ttgcggagaa cgaaacgtca
     7981 gatcgagcat ctatccagat acacgaagcg ttagcctggg gaatgcacat tcaattgtcg
     8041 ttgcagcgtc atttcctgaa cgccatcgaa ttgaagactc tcgcccgtct accgctaagc
     8101 tggtgcgacg aacaagggca gccgatctac cttcatcata atcgactttt aagttatgcc
     8161 gatatcgcac agttaagctc gggaatactg gtgttacagc ggaaatgttg tgtgaccgca
     8221 ctggctcgtg aagcggcaat tacccgaaat attcagttaa tcaggcagga gtaacctatg
     8281 catctggctc gagtcactgg cgcggtagta tccacgcaaa aatcaccttc tctgatcggg
     8341 aaaaaactgc tgctggttcg ccgggtcagt gctgacggcg aactgcctct cacgccatta
     8401 accggcgacg aagttgccgt tgattcagtc ggcgcaggcg ttggtgagtt ggtactcctc
     8461 agcagtggtt ccagcgccag acatgtgttt tcaggcccta acgaggccat cgacctggca
     8521 gtggtcggca ttgtcgatac gctttcgcgc taaggagcta aaagatggca atttataccc
     8581 gaacaggtga ttcaggaagt acgtctttat ttaccggaca gcgcgtcagc aaaactcatc
     8641 tacgtgtcga aacctatggc acgctggatg aactgaacgc gacactcagt ctgtgttatt
     8701 gcgccaccgc catcgaaagc catcgcatct tactggaagc cattcaacaa cagatatttt
     8761 ggtttagcgc cgaactcgcc agtgaaagcg aacagccgtc agcgcaacag cgttacatcg
     8821 gtacggaaga aattgccgcg ttggaaaacg cgatcgatag tgcaatgaac gccgtccccc
     8881 cagttcacag cttcattctg cctggtcgat gtgaagccgc aagccgcatg catttcgccc
     8941 gcacggtggc tcgacgggct gaaagacggc tggttgaact gacaacggaa accaccgtcc
     9001 ggaatgtttt gctgcactat atcaaccgac tttccgattg cctgtatgcc ctcgcccgcg
     9061 tggaagacaa cgttgctcat caaaacctga tgattcagga aatcacaaaa cgttatcacg
     9121 aagccaacca cacaccagca ttgaaggaac gcacaatgcc gctcactttt caggatcttc
     9181 accagctcat tcgtagtgcg gcaatgcgcg cggatgaact gcatattccc gttgtcatca
     9241 gcattgttga tgccaacgga acagaaagtg ttacctggcg gatgcctgat gccctgttgg
     9301 tcagcagtga actggcaccg aaaaaagcct ggaccgccgt ggcgatgaaa acggcaaccc
     9361 acaagcttgc tgataccgtg caacccgacg cgccgcttta cgggctcgaa agccatatgc
     9421 aaggaaaagt cgtcactttt ggtggcggct tccccctctg gcgtgacgga aaattgcttg
     9481 gcgggcttgg catcagcggc ggtagcgttg aacaagacat ggatattgcc caaagcgcaa
     9541 tggcggcaat taacgtggga gtaaaccaat gaataactct gaattagaaa cactgatccg
     9601 caatattctg cgtgagcaac ttgtccccgc tacaccacga acgcaaagaa acgctatttt
     9661 ccagactgtc gatgaagcgg tctgcgcagc acactaggcc tttttacgct ttcaacagtg
     9721 cccgcttaaa acccgtagcg ccattatcag cgccattcgc gaagaattaa tgccccgcct
     9781 taccgaactg gcagaagaaa gcgccaaaga aacggggatg ggaaataaag aagataagta
     9841 cctaaaaaac aaagcggcac tcgataacac gccaggaatt gaagatttaa ccaccacagc
     9901 actaaccggt gatggtggca tggttttgtt tgagtactct ccgtttgggg tgattggctc
     9961 ggtcacgcca agcaccaacc ccacggaaac catcattaac aacagcatca gcatgctggc
    10021 agcaggtaat agcgtctact tcagtccaca cccgggtgcc aaaaacgttt cgttaaaact
    10081 gattaccaca atcgaagaca tcgcgtttcg ctgtagtggc attcataact tgatcgttac
    10141 cgtatctgaa ccgacctttg ctgccaccga acagatgatg gcgcacccta aaatcagcgt
    10201 tctggcgatt accggcggtc cggggatcgt cgccatggga atgaaaagtg gcaagaaagt
    10261 gattggtgcc ggagcgggta accctccgtg catcgtcgat gaaacggcgg atattgtgaa
    10321 agccgccgag gacatcatca acggcgcttc atttgattac aacctgccgt gtattgctga
    10381 aaaaagcctg attgtcgtgg catcagtggc agattattta attcagcaga tgcaaagttt
    10441 tggcgcactt cttcttaact atgagcaaac tgagaaattg cgtgcaattt gcctgccaga
    10501 cggtagcgcc aacaaaaaac tggttgggaa aagcccatca gccttgctgg aagcggcagg
    10561 cctccctttg cccgcaaaag cgccgcgcct actgattgct gttgtcgacg ccgatgatag
    10621 ttgggttact tgtgagcaac taatgccaat gctgcccatt gtgaaggtca aggattttga
    10681 cagcgctcta accctggcgc tgaaagtgga agacggcttg caccacacgg caattatgca
    10741 ctctcagaac gtctcacgac tcaaccttgc cgcgcgcgtt atgcaaacct caatatttgt
    10801 caaaaacggc ccctcctggg cgggtattgg cgtcggcggc gaaggcttta cgacctttac
    10861 cattgcaaca ccgactggag agggtacaac gtcagcacgg acttttgccc gctcacgacg
    10921 ctgcgtgtta accagcggct tttcaattcg ctagagaaac ggaaatgaat actttttcac
    10981 tgcagacgcg tttatacagc ggaccaggaa gtctcaaggt acttagccgt ttctctcata
    11041 gacacatctg gattatttgc gatgcctttc tggcacgctc accacttatt gacacgttgc
    11101 atcaggcgct gcccgacagt aatcgtctca gtatttttag cgaaataacg cccgccccaa
    11161 ctatccagac cgtggtgaaa ggcattgcgc aaatgcagac tttacgcccg gatgtagtta
    11221 tcggttttgg cggcggttct gccctggacg ccgcaaaagc aattgtctgg ttcggtcgcc
    11281 agtgtggcat tgaaattgaa acctgcgtag ccattcctac caccagcggt atcggttccg
    11341 aagtgaccag tgcttgtgtc atcagcgatc ctgagaaagg gattaaatat ccccttttcg
    11401 ataatgcact ttatccggat atcgcgatcc ttgatccatc tttggtcgtc agcgtgccac
    11461 cggcgataac ggccaatacc gggatggatg ttttaaccca tgcgcttgaa gcttatgttt
    11521 ctcctcgcgc cagcgacttc acggatgcac tggtcgaaaa agccgtgcaa attgtttttc
    11581 aatacctgcc cactgcagtg aaaaaaggcg attgtcttgc cacccgtggc aaaatgcata
    11641 atgcatcgac gcttgcagga atggccttta gccaggccgg actcggttta aaccatgcaa
    11701 ttgctcatca actcggcgga cagtttcatc tgccgcatgg actggcgaat gcgctcctgc
    11761 tcacggcagt tatccgcttt aatgctggcg atccgcgcac agccaaacgc tatgcccggc
    11821 tggcgaaaac gtgccattta tgcccggaca acgccaatga caccgccagc ctgaatgcac
    11881 ttattcaaca tattgagcag cttaatacaa cctgtacgct gccgactctt gccaatgcgc
    11941 tgaaagaaaa aaaagcagaa tggtcaatac gcataccaga tatggttcag gcggcgctgg
    12001 cggatgcaac gttgcgtacc aacccgcgtg cagctgatgc ctccgcgatt gcagaactgc
    12061 tcaaggggtt gttatgaacg tcggattaat ctcctgcgac gaccagacca tccgcgagcg
    12121 agtacaagca gcaggtgtcg tgggtgctgg cggcgcaggt tttccaaccc atattaagct
    12181 acaagcgcgt gctgacactt tcctggtgaa tgcggcggaa tgcgaaccca tgttgaaagt
    12241 agatcaacag ttaatggcgc agcaagccag ccgcctgctt cgcggtgttc actatgcgat
    12301 gaaagccacc ggggccagta gcggaatcat cgcgctgaaa gaaaaatatc aacgggcaat
    12361 aaacgcactc acaccactgt taccaccgga tatccgcctg catattttgc ccgacgttta
    12421 ccccgcagga gacgaagtgc tgaccatctg gatggcaact ggtcgccgcg ttccacccgc
    12481 tgcattaccc gttagcgtcg gtgtggtggt caataatgtt caaaccgtac ttaatattgc
    12541 cagagccgtc gagcaacagt atccagtgac gcgccgcaca ctcaccgtta atggtgcagt
    12601 ggcgaagcca ataacggtaa ccgtccccat cggcatgtct ttacgtgaag tcctggcgct
    12661 ggcgggcggc gcaacagtgg atgctcctgg ttttatcaat ggcgggccga tgatgggaag
    12721 tttgatcaca tcgctcgaca caccagtcag caaaaccact ggcggcttgc tggttttacc
    12781 caactctcat tcactgatcc aacgtcgaat gcagaatgac cgcagcgtac ttgccgtcgc
    12841 gaaaactgtt tgtgagcaat gccgcttgtg taccgatctc tgcccacgtc atttaatcgg
    12901 tcacgaactg gcaccccatt tattagtacg tgcgcggtta actaccaaca gctcgccacg
    12961 ccgcaactgt tactgacagc gctcacctgt tcagagtgca acgtctgcgc cagtgtcgcc
    13021 tgtccggtag gaatttcacc aatgcgcata aaccgcctgc ttaagcagga attacgcgca
    13081 caaaatctcc gctacgaagg tgccttaaat cctgcagatc ccatggcaaa ttatcgactg
    13141 attcccgtaa aacggctggt aaccagactt ggcttaaccc cgtggtatca ggatgcgcca
    13201 ttgtctgaac aggtgcctca gcccgaaaaa gtgacgttat tgttacgcca gcacatcggt
    13261 gccagcgcaa ttgcgtgtgt acaaaaaggc gatcgcgtcg ttcatgggca atgtgtaggt
    13321 caaattccac acggcacatt aggtgccccc attcatgcca gtattgatgg catggtcagc
    13381 gatgtgacag aaaacgcgat tacgcttgtg agaggttaac catgtcgaaa gcggtaggta
    13441 ttttagaact tagcagtatc gcgaaaggga tggaaaccgg cgacgccatg ctgaaaagcg
    13501 ccaacgttga gttactggtc agcaaaacgc tatgccccgg aaaatttctg ctgatgctcg
    13561 gtggcgatgt gggcgcggtg caacaggcca tcaacagcgg agcgtccctt gctggtgaaa
    13621 tgctggtcga cagtcttgtc ctgccgaata ttcatcccag cctcctttct gcaatcagcg
    13681 gacttaacca tgttgaacag caccgggctg tcggcgtagt cgaaacctgg agtgtcgccg
    13741 cctgcattga cgctgccgat tgtgctgtca aagctgcaaa tgtcattctt gttcgcgtac
    13801 atatggcttt tggcattggc ggtaaatgct acatggtggt tgctggcgat atttcagatg
    13861 ttaataacgc ggtctctgcc gccagcgtgc gcgcggggga aaaaggtttg ttggtctatc
    13921 ggtcggtgat tgcccgtcca catgaagcca tgtggcgtca gatggtggag ggataatgac
    13981 aactcagcac acaactgaac ggatgattca ggaatatgtc ccaggtaagc aggtcacgct
    14041 ggcacatgtc atcgccaatc cagggaagga tctgtttaag aaattgggtt taccagacac
    14101 catttccgct attggtattc tgacaattac ccccagcgaa gcatctatca tcgcatgcga
    14161 tatcgccaca aaatcaggcg ctgtagagat cggttttctt gatcgtttta ccggtgccgt
    14221 agtgctgact ggtgatgttt ctgcgatcga atatgcactt agccaggtta cccgtacgct
    14281 gggtgaaatg atgcgcttta ccgcctgccc cattacgcgg acctgacgtt atgcaacgta
    14341 tgatgctcat cggccccagc cagtgcggaa aaacgtcact cacgcaatgt ctgcgtggcg
    14401 aaaatattca ttatcagaaa acgcaggcaa tcgtctggtc cccgaccacc attgatacac
    14461 caggcgaata cctcgaaaac cgcagcctct acagtgcact gttggtcagt gcctgcgaag
    14521 cggacattat cgcgctggtg cttaatgcgg acgcaccgtg gtcacctttt tcgccaggct
    14581 ttacctcgtc aatgaatcgc ccggtcattg gagtagtaac caaagcagat ttagccaaca
    14641 tggaacaaat atctttggtg aaatgttggt tacgggaagc tggagcgcac aacgtgttag
    14701 taaccagtgc ggttaataac catggagttg cagaactttt agccttgctg catacagaag
    14761 aaggctgtcg ctaatttcga caggttatca gatgggaatg atgacggtga tacacagaaa
    14821 agaatcaatg agcaatgagg gttgccgggc aaccctcatt gaataaaaca ggaattactt
    14881 cgcttcgccg tttttcattt cgcccatagc tttcagcttt ttggagatat cgcggcgttc
    14941 tttggagagc tcggcatttt tgatgatgta atcgtcaacg cgatcttcat agtcaccttt
    15001 catgctggcg atgatgccct ggattgcttc aacgctcatc ccaggcttga tgtaatcgct
    15061 caggttgtcc agcagcagga cgcgcttctg gttgtcacgg atctttttct caacgtcctg
    15121 aatttcacgt tgcagtttgt tcttacgacg gaacagacga acaaattcca gtacgtcctg
    15181 aaatgaaggc ttggtagttt ccatttttat acccctgata atgtgagagt cggattcgtt
    15241 taagcaaccg ctattcgtta gggccaacat tactcatagc tgccgcagat gacaatgctt
    15301 ttatcccttt tcactatcat accctttatc cttgctgaat cgaagcagca gcaagatgat
    15361 tctgaagttc aggaattatt tgcgcaaggc ccggcaaatc agattcgata gcaactcaag
    15421 ctgatgcgcc gtttccatgt tcagccacac ataaccatag attggtgttt ccactacctt
    15481 caggtcatgg tggttattga gcaactgacg cagctcttcc acagcatcgt tcaatttttc
    15541 cgtattggca aaaaccggct gcggattacc ttcgtacagc gcatgaacaa agctcagcag
    15601 tatttgctgc atcatgtgct gggtatcacg aagtttttgc gcgttcaata acacgaaatg
    15661 gctggggcgc gtggcccagt acgcattgat ttgcaactcc agcatacaaa ccagattgcg
    15721 gttaatggtc tggatacctt catatatcga ttttggaata cgggtttctt tgctggcggg
    15781 cgcaatcagc ccacgcattt tcacggcatc ggtcaggagt ttttgtagat ggctttccag
    15841 acgtgggcgt tcgagtaagt tcggtgagaa tgcagattga tagacccgat tatactcggt
    15901 cagacttttc gccagttgaa tgcgccagtg gatgaacgcc cgttgtggcc agataccggt
    15961 aaacaacatt gccagtaaag agccgaggat cacatcgccg cttcgccata acgccgtatc
    16021 aatttcacct gtcggggaac caacaacaat tgccagcgtc accccaatca ataatccttg
    16081 atacggtttc ttgcccagcg ccagccaacc gcaaagaaac atcgccgccg cgcaccagac
    16141 taacatcagc ggtaacgaaa ttaactccag ttgcagagcg ataagaccta aaatcgaacc
    16201 caacaccgta ccgccaatac gttcgaaggc acgagggaca acgttacccc agaacgagat
    16261 tggccccata atcaccacca tggtgaccag cggccaggtg ctttccggga tagtaaacag
    16321 gcggataatg agaaaagtga gcaggaatgc cagcgcgacc cgagtaccat gcactatgcg
    16381 gtaatggcgg tatacccgga tttcaaacgg gcttaatgac ttatcggcac gcacgcagaa
    16441 actccgtaat gaagattaaa agcgctcata actcatctgt cggccagaag gcgcgcctct
    16501 gaattatccg acggacaaac cgggaacagt gtaatttccc ggcaggagcg aaaaaattga
    16561 aaggcgcatc ttactctttt cgctttcaaa aaaagagtgg tcatcgcgtt aacacaccgc
    16621 cctgagatga attagtgatg ttttggctgc accgcaacat acatttcgat atcccagtac
    16681 ccatcttccg cgccattgtt caaataaacc tcgaagcagg gctttggtaa catttcataa
    16741 gcactgtcct gcaagaggct attaaagaac tgataccagg gtttagcaaa atcatcaccg
    16801 actacacgag ccaccgccac cgcatactga ccacctgaaa tttctgtcag aatgacgccc
    16861 tcactgtttt cgggaagcgt aaagttattc ggtaccgtca caacggtgtc gcagcgtaat
    16921 ttttcggcgg gtgtttcatc tggattgtcg taatagacag caacccactc cttcggcaca
    16981 atatttttgt tatctaccca catcatcaac tgctcaaagc ctttctttac cgtctgttcc
    17041 cacgggccaa cgagatggaa acctgcaacg gtacgtttct cttcctgcgt aatctcgtag
    17101 ttcatgacgc ctccattaac actgttttta tatacagcat agttgcaata ttaaaaccac
    17161 aaggaatgag tgttgattat gcgagcagac tcgcactcct gccagtctgc tgcaaaagaa
    17221 aggtcaggcc ttatggtgga aataatcact caggcgggaa aacatgccgc cttccccgac
    17281 agattccagg gtaaccagcg gccagtgcgc cacctgttta tcacggtcgt aaagttcaat
    17341 ttcccctacc cgctgatggg cgctaattgg cgcggtgagc tctttaccat caagggtata
    17401 tttggctttg atatgtggaa tttcggcttt cggtagcacc atccagaact cttgttccgt
    17461 tcctaggtcg atattttctt tatcgccata ccagatgcgt tccgttccga cctttttccc
    17521 acggtgcaaa atttgcaccg tagtaaagtt ttgttgcccc caacgcagta attttcttgc
    17581 ctcttcctca cgaccttttg cactgtcagc ccccattacc actgcaatga gacgacgctg
    17641 cccatctaca gccgaagcaa tgagattaaa cccggcacca gaagtatgac ccgttttcag
    17701 gccgtcaaca ttcatggttt tatcccacaa caacccgtta cggttttgct gggtgatacc
    17761 gttccaggtg agacttttct cactgtacat atgataaaac tcgggctcgc cgtggatgat
    17821 agcgcgagaa aacacagcta aatcataagc cgagctatgc tggccaggtg catccagacc
    17881 atgcactgtt tcaaaatgcg tatccttgag atgcagcttc tcggcatagt tgttcatcat
    17941 ttcaacaaac tgccgttgcc caccggcaat atagtcagcc agagcaacac aagcgtcatt
    18001 tccggaatcc acaattaaac cacggcttaa atcacgtacc gatacgcgat cgccctcttt
    18061 caaaaacatc agtgaagaac cgacaaacac cggattatct ttcgcccacg catcgcgccc
    18121 cacggtgaca atatcgtctg gcgtaatgcg atgactatcg atagcgcgat ccacgacata
    18181 acccgtcatc agctttgtca ggctggcggg attgcgctgt tgatgctcat tacccgcggt
    18241 gaggatctga ccggtggtgt aatccatcaa tacccaggac ccggcatgaa tctctggagg
    18301 ctgaggtgaa aaaggaatgt tttccgccgc aaaaccagac gataagttaa aaacgaacaa
    18361 agaagcagca ataataagac ggcgtttcaa cagcaaaccc tcaggagttt caaatagctg
    18421 ttctttttac ggaaatactt atgaactggc tggaataaag tgcaagaaaa tgtgactacc
    18481 ctctcatttt tatctgacat gatctgttgc cactcgctgc caaattgtgg cgctaaagct
    18541 gattagcacg gtgatatttg atactctggc agacagcaga aataacggat ttaacctaat
    18601 gatgaatgac ggtaagcaac aatctacctt tttgtttcac gattacgaaa cctttggcac
    18661 gcaccccgcg ttagatcgcc ctgcacagtt cgcagccatt cgcaccgata acgaattcaa
    18721 tgtcatcggc gaacccgaag tcttttactg caagcccgct gatgactatt taccccagcc
    18781 aggagccgta ttaattaccg gtattacccc gcaggaagca cgggcgaaag gagaaaacga
    18841 agccgcgttt gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg
    18901 ctacaacaat gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta
    18961 cgatccttac gcctggagct ggcagcatga taactcgcgc tgggatttgc tggatgttat
    19021 gcgtgcctgt tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct
    19081 accgagcttt cgccttgagc atttaaccaa agcgaatgga attgaacata gcaacgccca
    19141 cgatgcgatg gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca
    19201 gccacgtctg tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat
    19261 tgatgttccg cagatgaaac ccctggttca cgtttccgga atgtttgggg catggcgcgg
    19321 caataccagc tgggtggcac cgctggcgtg gcatccagaa aatcgcaatg ccgtaattat
    19381 ggtggatttg gcaggagaca tttcgccatt actggaactg gatagcgaca cattgcgcga
    19441 gcgtttatat actgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt
    19501 gcatatcaat aaatgtccgg tgctggccca ggcgaatacg ctacgcccgg aagatgccga
    19561 ccgactggga attaatcgtc agcattgcct cgataacctg aaaattctgc gtgaaaatcc
    19621 gcaagtgcgc gaaaaagtgg tggcgatatt cgcggaagcc gaaccgttta cgccttcaga
    19681 taacgtggat gcacagcttt ataacggctt tttcagtgac gcagatcgtg cagcaatgaa
    19741 aattgtgctg gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgtcgataa
    19801 acggattgaa aagctgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta
    19861 tgccgagcag caacgctggc tggagcaccg ccgccaggtc ttcacgcccg agtttttaca
    19921 aggttatgca gatgaattgc agatgctggc acaacaatat gccgataaca aagagaaagt
    19981 ggcgctgtta aaagcgcttt ggcagtacgc ggaagagatc gtctaatttg aagcctccgc
    20041 cgctggtacg gcggaggtca atgttgagtc aggctttttg aacggtgata ctccacgctg
    20101 catcgccaat ctgctgataa tcggtgatgg catgcccttc ttctgccgcc cactgtggga
    20161 ttgcttcagt tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt
    20221 ctgccagtgc tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca
    20281 gctttttaat cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc
    20341 ttacgctgcg gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat
    20401 acaaggccaa tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata
    20461 aagcaaccac ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt
    20521 aatgttgttt gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata
    20581 aatgacccca cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca
    20641 cccgcgacca gaaattgcag aatattggcc gttggggaag tgattcctaa cccaaacatg
    20701 cgtccggttg cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca
    20761 gtgacaaagg gatgccagcg tttttcaaac agaatatgag cgatcccagt tcggcgcggc
    20821 ggtaaggtcg cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt
    20881 atcaccagca aaacggcaac caacggccaa acagacaaat taaaagtctc agcaatagag
    20941 ttatgttcag tactgtagtg ctgcaaggtt tgatttaaac cactggcatg tggagaacgc
    21001 atcaccgcac tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca
    21061 cccgcgcgat accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaag
    21121 atatacccac ctataacagt accaagccac gggaacgcct cggcttcgta agtcagtagg
    21181 cccgcctgaa ttaacgcaaa aacccccacg ctttgtaccg aaattgcaat cagcaaggcg
    21241 taaaacatgc gattattttt cgcgatatac atatcgcgaa aaccacctgt caggcaaaaa
    21301 cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt
    21361 gaaaacataa attaattaac cagatgaatg ttaatgagga aattattcat gactggtgga
    21421 atgc
//
LOCUS       DAZOYL010000038        21185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZOYL010000038 DAZOYL010000000
VERSION     DAZOYL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.038"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            64..624
                     /gene="nudE"
                     /locus_tag="ACHUOY_001064"
     CDS             64..624
                     /gene="nudE"
                     /locus_tag="ACHUOY_001064"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR0681905.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(789..3341)
                     /gene="mrcA"
                     /locus_tag="ACHUOY_001065"
     CDS             complement(789..3341)
                     /gene="mrcA"
                     /locus_tag="ACHUOY_001065"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR0681906.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            3461..4240
                     /gene="hofM"
                     /locus_tag="ACHUOY_001066"
     CDS             3461..4240
                     /gene="hofM"
                     /locus_tag="ACHUOY_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR0681907.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            4240..4779
                     /gene="hofN"
                     /locus_tag="ACHUOY_001067"
                     /pseudo
     CDS             4240..4779
                     /gene="hofN"
                     /locus_tag="ACHUOY_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            4763..5203
                     /gene="hofO"
                     /locus_tag="ACHUOY_001068"
     CDS             4763..5203
                     /gene="hofO"
                     /locus_tag="ACHUOY_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR0681908.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            5193..5597
                     /gene="hofP"
                     /locus_tag="ACHUOY_001069"
     CDS             5193..5597
                     /gene="hofP"
                     /locus_tag="ACHUOY_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR0681909.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            5509..6747
                     /gene="hofQ"
                     /locus_tag="ACHUOY_001070"
     CDS             5509..6747
                     /gene="hofQ"
                     /locus_tag="ACHUOY_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR0681910.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            7148..7669
                     /gene="aroK"
                     /locus_tag="ACHUOY_001071"
     CDS             7148..7669
                     /gene="aroK"
                     /locus_tag="ACHUOY_001071"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR0681911.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            7726..8814
                     /gene="aroB"
                     /locus_tag="ACHUOY_001072"
     CDS             7726..8814
                     /gene="aroB"
                     /locus_tag="ACHUOY_001072"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR0681912.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            8906..10192
                     /gene="damX"
                     /locus_tag="ACHUOY_001073"
     CDS             8906..10192
                     /gene="damX"
                     /locus_tag="ACHUOY_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR0681913.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            10299..11135
                     /gene="dam"
                     /locus_tag="ACHUOY_001074"
     CDS             10299..11135
                     /gene="dam"
                     /locus_tag="ACHUOY_001074"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0681914.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            11153..11830
                     /gene="rpe"
                     /locus_tag="ACHUOY_001075"
     CDS             11153..11830
                     /gene="rpe"
                     /locus_tag="ACHUOY_001075"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR0681915.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            11823..12581
                     /gene="gph"
                     /locus_tag="ACHUOY_001076"
     CDS             11823..12581
                     /gene="gph"
                     /locus_tag="ACHUOY_001076"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR0681916.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            12574..13578
                     /gene="trpS"
                     /locus_tag="ACHUOY_001077"
     CDS             12574..13578
                     /gene="trpS"
                     /locus_tag="ACHUOY_001077"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR0681917.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            13738..14643
                     /gene="yhfZ"
                     /locus_tag="ACHUOY_001078"
     CDS             13738..14643
                     /gene="yhfZ"
                     /locus_tag="ACHUOY_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR0681918.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            14660..15022
                     /locus_tag="ACHUOY_001079"
     CDS             14660..15022
                     /locus_tag="ACHUOY_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR0681919.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            15106..16269
                     /locus_tag="ACHUOY_001080"
                     /pseudo
     CDS             15106..16269
                     /locus_tag="ACHUOY_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            16269..17495
                     /locus_tag="ACHUOY_001081"
                     /pseudo
     CDS             16269..17495
                     /locus_tag="ACHUOY_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            17492..18370
                     /locus_tag="ACHUOY_001082"
     CDS             17492..18370
                     /locus_tag="ACHUOY_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR0681920.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            18381..18734
                     /locus_tag="ACHUOY_001083"
     CDS             18381..18734
                     /locus_tag="ACHUOY_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR0681921.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            18746..20050
                     /locus_tag="ACHUOY_001084"
     CDS             18746..20050
                     /locus_tag="ACHUOY_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR0681922.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            20062..21147
                     /locus_tag="ACHUOY_001085"
     CDS             20062..21147
                     /locus_tag="ACHUOY_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR0681923.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
BASE COUNT         4973 a         5263 c         5951 g         4998 t
ORIGIN      
        1 acattgccga aagcgtgtcc gatatcgcac aataaccgct gatttcacag catagatccc
       61 acgatgagca aatcattaca aaaacccacc attctgaatg ttgaaactgt agcccattcc
      121 cgactgttta ccgtcgagag cgtggatctg gagttcagca atggcgtgcg gcgtgtttat
      181 gaacgaatgc gtccaaccaa ccgggaagca gtgatgattg tgccgattgt ggacgatcac
      241 ctgatcctga tccgcgaata cgcagtggga actgaatcct acgaattagg tttttcgaaa
      301 ggattaattg atccgggtga aagcgtctac gaagccgcta accgcgagct aaaagaagag
      361 gttggatttg gtgcgaacga tctgactttt ttgaagaagc tcagcatggc accgtcttac
      421 ttttccagca aaatgaatat cgtggtcgcg caagatctct acccggaatc gctggaaggc
      481 gatgagccag agccgctacc acaggtgcgc tggccgctgg cgcatatgat ggatttgctg
      541 gaagaccctg acttcaacga agcgcgtaat gtcagcgcgc tgttcctcgt gcgcgaatgg
      601 ttgaaagggc aggggcgagt gtaaatgcgt gaagtttgcc tgatgcacaa cgattatcag
      661 gcttatgggg ttcctgcaat atattgattt ttcacgattt tgttagccgg ataacgcgtt
      721 cacccgcatc cggcataaac aaatgcactt tgtcagccaa ctgaaaaggc gccgaagcgc
      781 ctttttaatc agaacaattc ctgtgcctca ccattatcga taatggttgt tcccacctcg
      841 tgcaccgcct gttgtgtcgg ctgcgtacct tcgatgaaat actcttcgcg gctgttgcca
      901 ccattagcta actgcccggt gctgcgatcg atattcaccg tcacaatacc cggaggcggc
      961 gtcagcggct gctccggcac accttcaaga acggctttca tataagcgtc ccatgcaggc
     1021 tgggcactct tggcaccgcc ttcgtaacct gagatctgat ctttaatcgc tccggaagcc
     1081 gttgtatgac cgagattacg acggtgatca tcaaagccaa tccagaccga ggtcacaacg
     1141 cccggaccgt aacccgagaa ccacgcatct ttcgaactgt tagtggtacc ggttttcccg
     1201 ccgatatcgc gacgctgcaa atcacgacct gcacgccagc cggtaccctg ccagcctggt
     1261 tcaccaaaga tattggtgtt caaagcactc ttaatcagga atgccagcgg agtgttgatg
     1321 acgtgcggtg cgtactcctg cgcgccagtc ttcgccacta acgcctgatt tgcctgctcc
     1381 agctgcggca ttggtacaga aacattctgc tgctcgcggg agatagcgac atcttcaaca
     1441 tcgttatttt ccagcacgtt cgatttctgc gtatcaccgt aaatcaccgg aatatcgcat
     1501 tccgggcagg ctactttcgg tttcgcttcg aaaatcacgc cgccctgatc gttttcaatt
     1561 ttgctgataa accacgggtc caccaggaag ccgccgttcg ccatgaccgc gtagccgcgc
     1621 gccacctgca ttggggtgaa ggacgctgaa cccagcgcca gcgattcggt gtggacaatg
     1681 ttttgcgccg ggaagccgaa gcgttgcaga tattctgcag cgtagtcgac gcccatcgcc
     1741 cgcattgcac gtaccatcac cacgtttttc gactgaccca gcccctgacg taagcgaatt
     1801 ggaccagcat actgcggtgg tgagttcttc ggctgccagt cagaaccgac acctgcatcc
     1861 cagcgagaaa ttggcacatc gttcaacata cttgccagcg tcagaccttt atccatcgcc
     1921 gcggtgtaga ggaacggttt gatgttggaa cccacctgac gcagtgcctg ggtggcgcgg
     1981 ttaaacttgc tctgattgaa atcaaagcca ccgaccagcg ccataacggc accattttgc
     2041 ggattgatcg acaccagcgc cgagttcact tccggcactt gtgccagcca ccatgcatcg
     2101 ccaacctgac gaacccagat ttgctgaccc gtttgcagaa catcggtcac tttacgcggc
     2161 gtcggtccct gctgagtatc cgaacggtaa ggacgcgccc agcgaacgcc gtccatactc
     2221 aatgcgacag tcgacccgtc cgccagcatt gccgtcgctt cctgaggatt ggcgctggtg
     2281 actgcggcag gcagcagcgg accataggtc ggcagcgcct tcagcgtatc ggtaatcttg
     2341 ttgttatccc acgccgactc gcccactttc cacagcacat ttgccgggcc gcgatagccg
     2401 tggcgcatgt cgtagtccag cacgttatta cgtaccgcct gctgtgcggc ctgctgcact
     2461 ttgcgggtga tggtggtgta aatgcgataa ccgtcttcat aggcactttc gccataacgg
     2521 ttatacatct cctggcgcac catttcgctc aggtacggcg cagagaaagc aatctccggc
     2581 gcgtgatagt tagcgttaat cgcctcagtg cgtgtctgat cgaactgttg ttgggtgata
     2641 tacccttcat ccagcatccg cgacagtacg acgttacgcc gcgcgacggc acgatccatt
     2701 gagtagagcg gattaaaggt ggaaggcgct ttcggcagcc cggctatcac agccatttcg
     2761 ttcagcgtca gttggtcgac cgttttaccg aaatagactt gtgccgcagc accgacacca
     2821 taggcgcggt aaccgaggta aatcttgttc agataaagct cgaggatctc gtctttcgtc
     2881 agcaactgtt caatgcgaat cgcgaggaag acttccttaa tcttacgcat cagcgtgcgt
     2941 tctggactga ggaagaagtt tctcgccagc tgctgggtaa tggtactcgc cccttgtgac
     3001 gcgtggccgg agaacagcgc cacacttgcc gcacggaaga tccccaccgg gtcaacgccg
     3061 tgatgctcgt agaagcggct gtcttccgtc gcgataaagg ctttcaccat ctccggcggg
     3121 atttgatcca acgtaaccgg aatacgacgt ttctcaccgt attgagcaat cagctcgcca
     3181 tcggcgctgt aaatctgcat cggaatttgc aggcgaacat cttttaatgt cgccacatcc
     3241 ggtagttgtg gctcgatgta gcggtatagg ccataaatcg agcctgctcc cagcagaatg
     3301 caacagactg caaggatcaa aaaatacttt acgaacttca ctggaaattt cccatttagt
     3361 ttcatttggg cagtttataa acaaacgtgc ggtagtataa aggcaagcca gacgcattga
     3421 tatacccgtc agagtgacgg gtgataagga gatcatcaca atggcattta agatctggca
     3481 aattggtttg catttacaac agcaagaagc agtagcggtt gcgatcgtgc ggggcgcaaa
     3541 agaatgcttt ttgcaacgct ggtggcggtt gccgctggag aacgacatta tcaaagatgg
     3601 gcggattgtt gatgcgcagc ggctggctaa aacgttgtta ccgtggagtc gcgaactgcc
     3661 gcagcgtcat cacattatgt tggcgtttcc cgccagtcgc acattacagc ggtcatttcc
     3721 gcgcccgtcg atgtccctcg gtgagcggga gcaaacggcc tggctgtcag ggacgatggc
     3781 ccgcgagctg gatatggatc cggactccct gcgtttcgat tacagcgaag actcactcag
     3841 tcccgcctat aacgtgactg ccgcgcaaag caaagagctg gcaacgctac tcactctggc
     3901 agaaaggttg cgtgttcatg tgagcgcgat caccccggat gccagtgcat tacagcgatt
     3961 cctgcctttt ttaccttctc atcagcaatg tctggcctgg cgtgataacg aacagtggct
     4021 gtgggcgaca cgctgtcgct gggggcgcaa actggcggtg gggatgacta gcgcgaagga
     4081 gctggcggca gcgttatccg ttgatcccga aagcgtcgcg atatgtggcg aaggcggatt
     4141 tgatccctgg gaggccgttt ctgttcgtca gccgccgcta ccgccgccag gtggagactt
     4201 tgccatcgcg ctggggctgg cgcttgggaa ggcgtactga tgaacccgcc aattaatttt
     4261 ttgccctggc gacagcaacg ccggaccgct tttcttcgtt tctggttgct gatgttcgtt
     4321 gcgcctctgc tgctggccgt cgggataacg ctaatactgc gtctgacaga caacgccgaa
     4381 gcacgcgtaa acaccgtttt gcttcaggcg gaacaacaac tcgcccgcag cttacagata
     4441 acgaaaccac gtttgctgga gcgccaacaa attcgtgagc agcgtttgca acggcagcgc
     4501 cagcgacaat ttacccgcga ctggcaatct gcgctggaag cactggcggc gcttttaccc
     4561 gagcacgcct agctgacaac gataagctgg cagcagggaa cgctggagat caaggggctg
     4621 acaacaagca ttaccgcgtt aaacgcacta gaaacgtcac tccgccagga tgcgtctttt
     4681 catctcaatc agcggggagc cacgcagcag gatgcgcagg gacgctggca atttgagtat
     4741 cagttaacaa ggaaggttag cgatgaacat gttctttgac tggtggttcg ccacatcacc
     4801 ccgcctccgc cagttttgct gggcattctg gttgctgatg ttagttacgc tcatttttct
     4861 gtcatcgaca caccatgaag agcgcgacgc attaattcga ctacgggcaa gtcatcacca
     4921 gcagtgggcc acactgtatc gcctggttga caccactccc ttcagcgaag aaaaaacgct
     4981 gcccttttcg ccactggatt ttcagttgcc cggcgcgcaa ctggtttcct ggcatccatc
     5041 cgcgcaggga ggcgagttgg cgttgaaaac gctgtgggaa gcagtgccgt cggcatttac
     5101 acggctggca gagcgcaacg tcagcgtgag ccgtttttca ttaagcgtgg aaggtgatga
     5161 tcttttgttc acgctacaac tggagacgcc gcatgagggt taaacgctgg ttgttggcag
     5221 gtattgtatt gtgcctttta accggtatgc gtgacccttt taaaccgccg gaagatctat
     5281 gccggattag cgaacttagc cagtggcgct atcaggggat ggtagggcga ggcgagcgca
     5341 tcatcggtgt aataaaagac gggcaaaaga aatggcgacg ggtgcagcaa aacgatgtgc
     5401 tggaaaacgg ctggacaatt ttacagctga cgccagacgt actaacgctg ggtaccggga
     5461 caaactgcga accgccacaa tggttgtggc aacggcaagg agatacaaat gaagcaatgg
     5521 atagccgcac tactgttgat gctgataccc ggcgtacagg cggcaaagcc gcaaaaagtg
     5581 acgctgatgg tggatgacgt tccggtagct caggtgttgc aggcgctggc tgaacaggag
     5641 aagttgaacc tggtcgtgtc gccagacgtc agcggtacgg tgtcgttaca tctaacagat
     5701 gttccctgga agcaggcact acaaactgta gtgaaaagcg ccggactgat aacgcggcag
     5761 gaaggcaaca ttctctcagt gcattccatt gcctggcaga ataacaatat cgcccgccag
     5821 gaggcggagc aggcgcgggc gcaggcaaat ctgccgctgg aaaatcgcag tataaccctg
     5881 caatacgccg acgcgggaga actggcgaaa gcgggggaga agctactgag tgccaaaggg
     5941 agtatgaccg tcgataaacg caccaatcgc cttttgctac gagataacaa aacggcgtta
     6001 agcgcgcttg aacagtgggt agcgcaaatg gatctgccgg tcgggcaggt tgagctgtcg
     6061 gcgcatattg tcaccattaa tgaaaaaagt ttgcgtgagt taggcgtgaa atggacgctg
     6121 gccgatgcgc aacacgctgg tggcgttggg caagtcacca cgcttggtag cgacctctcc
     6181 gtagcgacgg cgacaacgca tgtcggtttt aacattgggc gcatcaacgg acgcttgctg
     6241 gatcttgagc tttccgcgct cgaacaaaaa cagcagctgg atattatcgc cagtccgcgt
     6301 ctgctggcct cacatcttca gcctgccagc attaaacagg ggagcgaaat tccatatcag
     6361 gtttccagcg gggaaagtgg cgcgacgtcg gtggaattta aagaggccgt cctggggatg
     6421 gaagttacgc ccacagtgtt acaaaaaggt cgcatccggc tgaaattaca catcagccag
     6481 aacgttccgg ggcaggtgct acagcaggcc gatggcgaag tgctggcgat tgataagcag
     6541 gagatcgaaa cgcaggtcga ggtcaaaagc ggagaaacgt tggcgctggg cggcattttt
     6601 acccgtaaaa ataaatcggg tcaggatagc gtaccgttgc ttggcgacat tccctggttc
     6661 gggcaattat ttcgtcatga cggaaaagaa gatgaacgac gcgagttagt ggtgtttatc
     6721 acgccacgac tggtttccag tgagtaaaca gccgtaaaag cggtaatgtt tttacgctga
     6781 acgtgtttca tctatttgac gcgcgcaggt atttagcata caaggagtac cgatttgaga
     6841 gttggtgctc ttcgctgcct gcgttccatg atgatgattt atcattcagg cggcattttg
     6901 ctgtcttttt tacgctaatc ttacccggtg atttatcgcc agagcggtgg tagcaaggca
     6961 gcgcgcttgc agcgaccaga tatgcagagg gatgggtgat ttattcagtt gccaaacccg
     7021 ctggagtatt gagataattt tcagtctgac tctcgcaata tcttatgagg tttcagttca
     7081 tgtcctgcgg cgctctctga gcgaagcggg tttatcatta acgaatagtc ttagtagtac
     7141 cgaaaaaatg gcagagaaac gcaatatctt tctggttggg cctatgggtg ccggaaaaag
     7201 cactattggg cgccagttag ctcaacaact caatatggaa ttttacgatt ccgatcaaga
     7261 gattgagaaa cgaaccggag ctgatgtggg ctgggttttc gatttagaag gcgaagaagg
     7321 cttccgcgat cgcgaagaaa aggtcatcaa tgagttgacc gagaaacagg gtattgtgct
     7381 ggctactggc ggtggctctg tgaaatcccg tgaaacgcgt aaccgtcttt ccgctcgtgg
     7441 cgttgtcgtt tatcttgaaa cgaccatcga aaagcaactt gcacgcacgc agcgtgataa
     7501 aaaacgcccg ttgctgcacg ttgaaacacc gccacgtgaa gttctggaag cgttggccaa
     7561 tgaacgcaat ccgctgtatg aagagattgc cgacgtgacc attcgtactg atgatcaaag
     7621 cgctaaagtg gttgcaaacc agattattca catgctggaa agcaactaat tctggcttta
     7681 tatacactcg tctgcgggta cagtaattaa ggtggatgtc gcgttatgga gaggattgtt
     7741 gttactctcg gggaacgtag ttacccaatt accatcgcat ctggtttgtt taatgaacca
     7801 gcttcattct taccgctgaa atcgggcgag caggtcatgt tggtcaccaa cgaaaccctg
     7861 gctcctctgt atctcgataa ggtccgcggc gtacttgaac aggcgggtgt taatgtcgat
     7921 agcgttatcc tccctgacgg cgagcagtat aaaagcctgg ctgtactcga taccgtcttt
     7981 acggcgttgt tacaaaagcc gcatggtcgc gatactacgc tggtggcgct tggcggcggc
     8041 gtagtgggcg atctgaccgg cttcgcggcg gcgagttatc agcgcggtgt tcgtttcatt
     8101 caagtcccga cgacgttact gtcgcaggtc gattcctccg ttggcggcaa aactgcggtc
     8161 aaccatcccc tcggtaaaaa catgattggc gcgttctacc agcctgcttc agtggtggtg
     8221 gatctcgact gtctgaaaac gcttcccccg cgtgagttag cgtcggggct ggcagaagtc
     8281 atcaaatacg gcattattct tgacggtgcg ttttttaact ggctggaaga gaatctggat
     8341 gcgttgttgc gtctggacgg tccggcaatg gcgtactgta ttcgccgttg ttgtgaactg
     8401 aaggcagaag ttgtcgccgc cgacgagcgc gaaaccgggt tacgtgcttt actgaatctg
     8461 ggacacacct ttggtcatgc cattgaagct gaaatggggt atggcaattg gttacatggt
     8521 gaagcggtcg ctgcgggtat ggtgatggcg gcgcggacgt cggaacgtct cgggcagttt
     8581 agttctgccg aaacgcagcg tattataacc ctgctcaagc gggctgggtt accggtcaat
     8641 gggccgcgcg aaatgtccgc gcaggcgtat ttaccgcata tgctgcgtga caagaaagtc
     8701 cttgcgggag agatacgctt aattcttccg ttggcaattg gtaagagtga agttcgcagc
     8761 ggcgtttcgc acgagcttgt tcttaacgcc attgccgatt gtcaatcagc gtaacaacaa
     8821 gaaaggtcag gccgcttatc aagcggtcta ttagcttcag gttaattgca acgtggtaag
     8881 cattaacctt ttagtggggt gttaaatgga tgaattcaaa ccagaagacg agctgaaacc
     8941 cgatcccagc gatcgtcgta ctggtcgttc tcgtcaatct tctgaacgtt ctgagcgtac
     9001 tgaacgtggc gaaccgcaga tcaattttga tgatattgaa cttgatgaca ctgacgatcg
     9061 ccgtccgact cgtgcgcaaa aagagcgcaa tgaggaaccg gaaatcgaag aagaaattga
     9121 cgaatccgaa gatgaaaccg tggatgaaga gcgcgtagag cgtcgtccgc gtaagcgcaa
     9181 aaaagcagcc agtaaacccg cttctcgtca gtatatgatg atgggcgtcg gcattctggt
     9241 tctactgctg ttgatcatcg gtatcggttc tgcgctaaaa gccccctcga cctcttccag
     9301 cgatcaaacc gcgtctggcg agaagagtat tgatcttgca ggcaatgcga ccgatcaggc
     9361 gaatggggtg cagccagcgc cgggaaccac gtctgcggaa aatactcagc aggatgtttc
     9421 tctgccaccg atctcttcta cgccgactca agggcaaacc ccggcggcaa cggatggtca
     9481 acaacgtgtt gaagtgcagg gtgacctgaa caatgcgctg acccagccac aaaatcagca
     9541 acagttgaac aatgtggcgg tcaattccac attgccgacc gaacccgcga ctgtcgcgcc
     9601 tgttcgcaat ggcaatgcat cgcgtgacac ggcgaaaacg caaaccgctg aacgtccgtc
     9661 cactacgcgc ccagctcgtc agcaggcggt gattgaaccg aaaaaaccgc aagcaaccgt
     9721 gaaaacggag ccgaagccgg tagcacagac gccgaagcgt actgaaccag ctgcccctgt
     9781 ggcgagcacg aaggcaccga ctgcgacttc tacgccagca ccaaaagaga cggcgactac
     9841 ggctccagta cagacggcat ccccggcgca aaccacggca acaccagccg ctggagggaa
     9901 gaccgcaggt aatgttggtt cgttgaaatc ggcaccgtcc agccattaca ctctgcagct
     9961 gagcagttcc tctaactacg acaacctgaa cggttgggcg aagaaagaga atctgaaaaa
    10021 ctacgttgtc tatgaaacgg cgcgtaatgg tcagccgtgg tatgtcctgg tttctggcgt
    10081 gtacgcttcg aaagaagagg cgaaaaaagc ggtatctaca ttgccagcag atgttcaggc
    10141 caaaaacccg tgggcgaaac cgctgcgtca ggtacaggcc gatctgaagt aatcaaggtt
    10201 atctcccgca atggtttatc gttgcgggag ctgcctgaag cgctggatgc tgtcggagct
    10261 ttctccacag ccggagaagg tgtaattagt tagtcagcat gaagaaaaat cgcgcttttt
    10321 tgaagtgggc agggggcaag tatcccctgc ttgatgatat taaacggcat ttgcccaagg
    10381 gcgaatgtct ggttgagcct tttgtaggtg ccgggtcggt gtttctcaac accgactttt
    10441 ctcgttatat ccttgccgat atcaatagcg acctgatcag tctctataac attgtgaaga
    10501 tgcgtactga tgagtacgta caggccgcac gcgagctgtt tgttcccgaa acaaattgcg
    10561 ccgaggttta ctatcagttc cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg
    10621 cggtactgtt tttatatttg aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc
    10681 gcggtgagtt taacgtgccg tttggccgct acaaaaaaac ctatttcccg gaagcagagt
    10741 tgtatcactt cgctgaaaaa gcgcagaatg cctttttcta ttgtgagtct tacgccgata
    10801 gcatggcgcg cgcagatgat gcatccgtcg tctattgcga tccgccttat gcaccgctgt
    10861 ctgcgaccgc caactttacg gcgtatcaca caaacagttt tacgcttgaa caacaagcgc
    10921 atctggcgga gatcgccgaa ggtctggttg agcgccatat tccagtgctg atctccaatc
    10981 acgatacgat gttaacgcgt gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc
    11041 gacgcagtat aagcagcaac ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt
    11101 acaaaccagg agtcgtttca cccgcgaaaa aataattctc aaggagaagc ggatgaaaca
    11161 gtatttgatt gccccctcaa ttctgtcggc tgattttgcc cgcctgggtg aagataccgc
    11221 aaaagccctg gcagctggcg ctgatgtcgt gcattttgac gtcatggata accactatgt
    11281 tcccaatctg acgattgggc caatggtgct gaaatccttg cgtaactatg gcattaccgc
    11341 ccctatcgac gtacatctga tggtgaaacc cgtcgatcgc attgtgcctg atttcgctgc
    11401 cgctggtgcc agcatcatta cctttcatcc agaagcctcc gagcatgttg accgcacgct
    11461 gcaactgatt aaagaaaatg gctgtaaagc gggtctggta tttaacccgg cgacacctct
    11521 gagctatctg gattacgtga tggataagct ggatgtgatc ctgctgatgt ccgtcaatcc
    11581 tggtttcggc ggtcagtctt tcattcctca aacactggat aaactgcgcg aagtacgtcg
    11641 ccgtatcgac gagtctggct ttgacattcg tctggaagtg gacggtggcg tgaaggtgaa
    11701 caacattggc gaaatcgctg cggcgggcgc ggatatgttc gtcgccggtt cggcaatctt
    11761 cgaccagcca gactacaaaa aagtcattga tgaaatgcgc agtgaactgg caaaggtaag
    11821 tcatgaataa gtttgaagat attcgcggcg tcgcttttga tcttgatggt acgctggtcg
    11881 acagtgctcc cggtcttgct gctgcggtag atatggcgct gtatgcgctg gagttgccca
    11941 tcgcaggtga agaacgcgtt attacctgga ttggtaacgg cgcagatgtt ctgatggagc
    12001 gcgcattgac ctgggcgcgt caggaacgtg cgactcagcg taaaacaatg ggtaaaccgc
    12061 ccgttgatga cgacattccg gcagaagaac aggtacgtat tctgcgtaaa ctgttcgatc
    12121 gctactatgg cgaggttgcc gaagagggga cgtttttgtt cccgcacgtt gccgatacgt
    12181 tgggcgcgtt gcaggctaaa ggcctgccgc taggcctggt caccaacaaa ccgacgccgt
    12241 tcgtcgcacc gctgctcgaa gccttagata ttgccaaata ctttagcgtg gttatcggcg
    12301 gcgatgatgt gcaaaacaaa aaaccgcatc cggacccgct gttactggtg gctgagcgga
    12361 tgggaattgc cccacaacag atgctgtttg tcggcgactc acgcaatgat attcaggcgg
    12421 caaaagcggc aggttgccca tcagttggct taacctacgg atataactac ggcgaggcta
    12481 tcgatctcag ccagcctgat gtaatttatc agtctataaa tgaccttctg cccgcattag
    12541 ggcttccgca tagcgaaaat caggaatcga aaaatgacta agcccatcgt ttttagtggc
    12601 gcacagccct caggtgaatt gaccattggt aactacatgg gtgcgctgcg tcagtgggta
    12661 aacatgcagg atgactacca ttgcatttac tgtatcgttg accaacacgc gatcaccgtg
    12721 cgccaggatg cacagaagct gcgtaaagcg acgctggata cgctggcctt gtatctggct
    12781 tgtggtatcg atcctgagaa aagcaccatt tttgttcagt cccacgtacc agaacatgcg
    12841 cagttaggct gggcactgaa ctgttatacc tacttcggcg aactgagtcg catgacgcag
    12901 tttaaagata aatctgcgcg ttatgccgag aacatcaacg ctggtctgtt tgactatccg
    12961 gtgctgatgg ctgcggacat cctgctgtat caaactaatc tggtaccggt gggtgaagac
    13021 cagaaacagc acctggaact gagtcgcgat atcgcccagc gtttcaacgc gctgtatggc
    13081 gagatcttta aggtgccgga gccgtttatt ccgaaatctg gcgcgcgcgt aatgtcgctg
    13141 ctggagccga ccaagaagat gtccaagtct gacgataacc gcaataacgt tatcggcctg
    13201 ctggaagatc cgaaatcggt agtgaagaaa atcaaacgcg cggtcactga ctccgacgag
    13261 ccgccggtag ttcgctacga tgtgcagaac aaagcgggcg tttccaacct gctggatatc
    13321 ctttctgcgg taacgggcca gagcatccca gaactggaaa aacagttcga aggcaagatg
    13381 tatggtcatc tgaaaggtga agtggctgat gccgtttccg gtatgctgac tgaattgcag
    13441 gaacgctatc accgtttccg caacgatgaa gccttcctgc aacaggtgat gaaagacggc
    13501 gcagaaaaag ccagtgtaca cgcttcccgt acgctgaaag cggtgtacga agcgattggt
    13561 tttgtggcga agccgtaatt taacattaat gccaaaaacc ggggaaaccc ggttttttta
    13621 tcctcatttg caataatccg aaaaaatgtg aagcgcctcg ccgtttccac atctcgtcgt
    13681 tgccacttat cttcatttta atgaagatat aaatattcat tttattgaaa atatattatg
    13741 cgtcgaacgt ttatcaaaaa agaaggcgtc gtcatcacga cgctggcccg ttatttgttg
    13801 ggtgaaaaat gcggtaatcg actgaaaacc atagatgagc tggcaactga atgccgttca
    13861 tcggttggcc tgacgcaggc cgcgttgaaa acgttggaat caagcggagc gatacggatt
    13921 gaacgccgtg ggcgcaatgg cagttatctg gtcgagatgg ataacaaagc attgctgagt
    13981 catgtggata tcaacaacgt ggtatgtgcg atgcctttgc cctatacccg cttgtacgaa
    14041 ggtttagcga gcgggctgaa agctcagttt gatggcattc ctttttacta tgcgcatatg
    14101 cgcggcgcgg atattcgcgt ggagtgttta cttaacggcg tgtatgacat ggcggtggtt
    14161 tcccgactgg cggcggaaag ttatctcacg caaaaagggt tatgcctcgc gctggagttg
    14221 gggccgcaca cctacgttgg cgagcaccag ttgatttgcc gtaaaggcga gtccgcaaac
    14281 gtgaagcgcg tggggctgga taaccgttcg gcggatcaga aaatcatgac cgatgtcttt
    14341 tttggcgata gtgatgtgga acgtgtcgat ctctcttatc acgagagttt acaacgcatt
    14401 gttaaaggcg atgtcgatgc ggttatctgg aacgtggtgg cggaaaacga actgaccatg
    14461 ctgggattag aggcgacgcc gctcacagac gatccgcgat ttttacaggc caccgaagcc
    14521 gtggtcctga cgcgagtcga tgattaccca atgcaacaac tgctgcgtgc cgttgtagat
    14581 aaacacgccc tgctcgccca tcaacaacgg gtagtgagtg gggaacagga accgagttat
    14641 taaacgaaag gcatctgata tggaaaccag actcaacctg ctttgcgagg caggcgttat
    14701 tgataaggac atctgcaaag gcatgatgca ggtcgtcaac gtactggaaa cagagtgcca
    14761 tctgccggtg cgcagtgagc aaggaacgat ggcgatgaca catatggcga gtgcactgat
    14821 gcgcagtcgc cgtggtgaag aaatagagcc gctggataac gaattgctgg cagagctggc
    14881 gcaatccagc cactggcaag ccgttgtgca attgcatcag gtgttgttga aggaattcgc
    14941 actggaagtt aacccgtgtg aagaaggcta tttgctggcc aacctttatg gattatggat
    15001 ggctgctaac gaagaggttt gaagctcccg acaaatccac gtcgcacaac ctaaatattc
    15061 aaaaaaatga atatttaatt caaggaacag aaacgaggca acaagatgtt tgtagaagca
    15121 ttgaaacgcc agaacccggc gctgatttcc gccgcactaa gtttgtggca gcagggcaag
    15181 atcgccccgg acagctgggt gatcgacgtg gatcaggtac tggaaaacgg taagcgactg
    15241 attgagacgg cgcggcttta cggcattgaa ctgtatctga tgaccaagca gtttggccgt
    15301 aacccatggc tggcggaaaa attgctggca ttaggttaca gcggcattgt ggcggtggat
    15361 tacaaagagg cgcgagtcat gcgccgcgct ggtttgtcgg tggcacatca ggggcatctg
    15421 gtacaaatcc cttgtcatca ggttgctgac gccgttgaac agggcaccga cgtcatcacc
    15481 gtgtttactc tcgacaaagc gcgggaagtt tctgcggcag cggtgaaggc cggacgtgtg
    15541 cagtttgtgc tgctcaaagt ttatagcgac gatgattttc tttatccggg gcaggagagc
    15601 ggttttgtac agcattcgct gcacgaggtg gttgctgaaa ttaagaaact gccggggctg
    15661 catttagccg gacttaccca tttcccttgc ctgctttggg atgaggctgc cggaaaagtt
    15721 ttgccgacac cgaatcttca cacgctgata caggcacggg atcaactggc gaaatctggt
    15781 attgcacttg agcaactgaa cgcgccttca gcgaccagct gcacttcgct gccattactg
    15841 gcgtaatacg gtgtgactca tgccgaaccc ggtcatgcgc tgacgggcac tattccggca
    15901 aaccagcagg gcgatcagtc tgaacgtatc gcgatgctct ggttgagtga aatctcccat
    15961 catttccgtg gcgacagcta ctgctacggc ggcggttact accgtcgtgg tcatgcgcaa
    16021 catgcgttgg tgtttacgcc agaaaatcaa aagattactg aaaccaatct caaaactgtg
    16081 gatgacagca gtatcgacta caccctgtcg ctggcaggcg agtttccggt aagcagtgca
    16141 gtggtgctct gtttccgcac gcagattttt gtcacccgta gcgatgtggt gctggtgtcc
    16201 ggtattcacc gtggcgaacc gaaaatcgtt ggtcgttatg acagtcttgg aaactctctg
    16261 ggggcgtaat ggcgcgattt gtggtgttag tgattgatag ctttggcgta ggggcaatga
    16321 aagatgtcac gctggtgcgt ccgcaagatg cgggagcgaa tacatgtggt cacatcctga
    16381 gccagttgcc gcatttgcag ctaccaacgc tggagaagct ggggctaatc aacgcattgg
    16441 gttatgcgcc aggcgatatg cagccgtcag attccgcaac ctggggcgtg gcagagctgc
    16501 aacatgaagg tggcgatacc tttatggggc atcaggaaat tttaggcacg cgcccgttac
    16561 cgccgctgcg gatgcctttt cgcgatgtga ttgaccgtgt tgagcaggca ttagtatccg
    16621 ccggttggca ggtggagcgc cgtggcgatg aactgtaatt tctgtgggtc aatcaggcgg
    16681 ttgcgattgg cgataatctc gaggcggatt taggccaagt ctataacatt accgccaatc
    16741 tctctgtgat ctcttttgac gacgcaatca aaattggtcg tatcgtgcgt gagcaggtac
    16801 aggtcggtcg ggtcattaca tttggtggcc tgttaaccga cagtcaacgc attctcgatg
    16861 ccgcagaaag caaagaaggg cgctttattg gtatcaatgc gccgcgttct ggcgcttatg
    16921 acaacggctt ccaggtcgtg catatgggct acggtgtcga taaaaaagtg caggtgccgc
    16981 aaaaactgta tgaggcgggc gtgccaaccg tgctggtggg taaggtggca gatatcgtca
    17041 gcaatcctta tggcgtgagc tggcaaaacc tggtggatag ccagcggatt atggatatca
    17101 ccctcgacga atttaacacc catccgacgg cgtttatttg caccaatatc caggaaaccg
    17161 acctcgctgg tcatgcagaa gacgtcgcac gttatgccga acgtttgcag gtcgttgacc
    17221 gtaaccttgc ccggcttgtt gatgcgatgc agccagatga ttgcctggtc gtgatggcgg
    17281 atcacggcaa cgatccgacc attggtcaca gccaccatac ccgcgaagtg gtgccgctgc
    17341 tggtttatca gcaaggcctg gctgctacgc agctcggtgt gcgcaccacg ctttctgatg
    17401 tgggagctac cgtgtgtgaa tttttccgtg cgccaccgcc acaaaatggt cgctcttttc
    17461 tttcctccct ccggtttgca ggagacaccc tatgagtttt gatccgacgg gttacaccct
    17521 ggcccatgag catctgcata ttgatctctc cggctttaaa aacaacgtgg actgccgcct
    17581 tgatcagtat gcgttcattt gccaggagat gaacgacctg atgacccggg gtgtacgtaa
    17641 tgtgattgag atgaccaacc gttacatggg gcgcaatgcg caatttatgc tcggtgtaat
    17701 gcgcgaaacc gggatcaacg tggtggcctg tactggttat taccaggatg cgtttttccc
    17761 ggaacatgtg gtgacccgca gcgtgcagga actggcgcag gagatggtcg atgaaattga
    17821 acagggtatc gatggcacgg agctgaaagc cgggatcatc gcggagatcg gctccagcga
    17881 aggaaagatt acgccgctgg aagagaaggt atttattgcc gctgcgctgg cgcataacca
    17941 gaccggacgc ccgatctcca cgcatacgtc gttcagcacc atggggctgg aacaactggc
    18001 tctgttgcaa gcccacggag ttgatctttc gcgcgtcacc gttggtcact gcgatctgaa
    18061 agacaacctc gacaacattt tgaagatgat cgatctcggc gcgtacgtgc agttcgacac
    18121 cattggtaag aacagttact acccggacga aaagcgcatt gcgatgctcc acgcgctacg
    18181 tgaccgtggg ttgctgaacc gcgtcatgct gtcgatggat attacgcgcc gctcccattt
    18241 aaaagccaat ggtggttatg gctatgacta tttattgacc acctttattc cgcaattgcg
    18301 ccagtcagga ttcagtcagg ccgatgtgga tgtgatgtta cgtgaaactc cctctcaatt
    18361 tttccaataa ggacagactc atgaaaaaga ttggcgttgc aggcttacag cgtgagcaga
    18421 ttaaaaaaac cattgaagcg acggctactg gcagttttga agttttcatt cacaacgaca
    18481 tggaagcggc aatgaaagtg aaatccgggc aactggatta ctacatcggt gcgtgtaata
    18541 ccggtgcggg cgcggcattg tcgattgcca tcgcggtgat tggctataac aaaagttgca
    18601 ccattgccaa accaggcatt aaagcgaaag acgagcatat cgccaaaatg atcgctgaag
    18661 gaaaagtggc gtttggcctt tccgttgagc acgtcgaaca cgcgattccg atgctgatta
    18721 accatctgaa ataaaaggca cgactatgga tctgtatatt cagattatcg tggtggcgtg
    18781 cctgacgggt atgacatcgc ttctggcgca tcgctcggcg gctgtttttc atgacggtat
    18841 ccgcccgatc ctgccgcaac tgattgaagg ctatatgaac cgtcgcgagg cggggagtat
    18901 cgcttttggt ctgagcattg gttttgtggc ctcggtgggg atctctttta ccctgaaaac
    18961 cgggctgctc aacgcatggt tactctttct tcctaccgat atcctcggcg tactggcgat
    19021 aaacagcctg atggcgtttg gtcttggcgc tatctggggc gtgttgatcc ttacttgcct
    19081 gttgccagta aaccagctgc tgaccgcgct gccggtggat gtattaggta gcctcgggga
    19141 attaagctcg ccggtggttt ctgcttttgc actgttcccg ctggtggcga ttttctacca
    19201 gtttggctgg aagcaaagtc tggtcgccgc cgtggttgtt ctgatgaccc gtgtggtagt
    19261 cgtgcgctat ttcccacatc ttaaccctga atccatcgaa atctttattg gcatggtgat
    19321 gctgctgggg atcgcgataa ctcacgacct gcgtcatcgt gatgaaaatg atattgatgc
    19381 cagcgggctt tcggtgtttg aagaacgcac gtcgcggatt atcaaaaact taccctatat
    19441 cgccatcgtg ggagcattga ttgccgccgt tgccagcatg aagattttcg ccggcagtga
    19501 agtgtcgatc ttcactctgg agaaagcgta ctccgcaggc gtaacgccgg aacaatcgca
    19561 aacgctgatc aaccaagcgg cactggcaga atttatgcgc gggctgggtt ttgtaccgtt
    19621 gattgccacc accgccttag caacgggtgt gtatgcagtt gcgggcttta cctttgttta
    19681 tgcggtgggc tatctctcgc cgaatccgat agttgcagcg gtattaggcg cagtggttat
    19741 ttcggcggaa gtcttgctgc ttcgttcgat cggcaaatgg ctgggacgct acccgtcggt
    19801 gcgtaatgcg tcggataaca tccgtaacgc catgaatatg ctgatggaag tggcgctgct
    19861 ggtcggttcg attttcgcag caattaagat ggcgggttat accggattct ctatcgcggt
    19921 tgccatttac ttcctcaacg aatccctggg ccgtccggta cagaaaatgg cggcaccggt
    19981 cgtggcagta atgatcaccg gtattctgct gaatgttctt tactggcttg gcctgttcgt
    20041 tccggcttaa ggaaggcacc tatgaagacg tttcctctgc aaagtctgac gattattgag
    20101 gcgcagcaaa agcagtttgc gctggtggat agcatttgtc gccatttccc cggcagcgag
    20161 tttcttactg gcggtgattt aggcttaacg ccaggactga atcaaccgcg cgttacccag
    20221 cgtgtggaac aggtgctggc tgatgcattt cacgcacagg ctgcggcgct ggtgcagggt
    20281 gcgggtactg gcgcgattcg tgcggcgttg gcggctttgc tcaaaccggg gcagcgtctt
    20341 ctggtacatg atgcgcctgt ttacccgacg acacaggtca ttattgagca gatggggctg
    20401 acgcttatta ctgctgattt caatgacctg tcggcactga agcaggtcgt cgacgagcaa
    20461 ctaccggatg cggcgctggt gcagcatacg cgccagcagc cgcaggacgg ctacattctg
    20521 gcagatgtgc tggcaacatt gcgctcggct ggcgttcctg cattaaccga tgacaactat
    20581 gcggtgatga aagtggcgcg aattggctgc gaatgcgggg caaatgtctc gactttttcc
    20641 tgcttcaagc tgtttgggcc ggagggtgtt ggcgcggtgg taggcgatgc cgatgttatt
    20701 agtcggattc gctccacgct ctattccggc ggcagccaga ttcagggcgc tcaggcgctg
    20761 gaagtcttgt gtgggctggt gtttgcgcca gtgatgcacg cggtgcaggc gggggtatct
    20821 gaacggttgc tggctttgct taacggtggt gcggtgccgg aagtgaaaag cgcggtgatt
    20881 gctaatgcgc agtcgaaggt gttgattgtc gagtttcatc agccgattgc cgccagagtg
    20941 ctggaagagg cgcaaaagcg cggtgccttg ccttacccgg tgggtgcaga gtcgaaatat
    21001 gaaatcccgc cgctctttta tcgcctttcc ggaacgtttc gccaggcgaa tccacaatca
    21061 gaacattgtg cgattcgcat taacccgaat cgcaacggtg aagagacggt gctgcggatt
    21121 ttgcgtgaga gtattgccag tatttaatgc tggatagcac cgtcaagcta aattccgtac
    21181 tgaac
//
LOCUS       DAZOYL010000039        21184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZOYL010000039 DAZOYL010000000
VERSION     DAZOYL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21184
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.039"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHUOY_001086"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHUOY_001086"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR0681924.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHUOY_001087"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHUOY_001087"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR0681925.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHUOY_001088"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHUOY_001088"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR0681926.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHUOY_001089"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHUOY_001089"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR0681927.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHUOY_001090"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHUOY_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR0681928.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHUOY_001091"
     CDS             complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHUOY_001091"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR0681929.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHUOY_001092"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHUOY_001092"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR0681930.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLCLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="ACHUOY_001093"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="ACHUOY_001093"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR0681931.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHUOY_001094"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHUOY_001094"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR0681932.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="ACHUOY_001095"
     CDS             complement(12799..14139)
                     /locus_tag="ACHUOY_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR0681933.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHUOY_001096"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHUOY_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR0681934.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="ACHUOY_001097"
     CDS             complement(15928..16377)
                     /locus_tag="ACHUOY_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR0681935.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHUOY_001098"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHUOY_001098"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR0681936.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="ACHUOY_001099"
     CDS             complement(17177..17506)
                     /locus_tag="ACHUOY_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR0681937.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHUOY_001100"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHUOY_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR0681938.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="ACHUOY_001101"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="ACHUOY_001101"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR0681939.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHUOY_001102"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHUOY_001102"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR0681940.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5167 a         5687 c         5056 g         5274 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cttggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 tcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc acaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaagcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gagaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgtgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagggtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    18961 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggac atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgcgttgc
    19981 aggccaactt gttcgcggtt ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcactgcatg tcggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggaca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaatcagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcacagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgttcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tgcatacgtt gggtgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    20761 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacatacc
    21121 catcaattta tttcatttgt tatatgaatg tttcttacca ccctcacgcg ataaatatca
    21181 ttgc
//
LOCUS       DAZOYL010000040        20711 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZOYL010000040 DAZOYL010000000
VERSION     DAZOYL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.040"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUOY_001103"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUOY_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR0681941.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUOY_001104"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUOY_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR0681942.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUOY_001105"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUOY_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR0681943.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUOY_001106"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUOY_001106"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR0681944.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUOY_001107"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUOY_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR0681945.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHUOY_001108"
     CDS             complement(8214..9107)
                     /locus_tag="ACHUOY_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR0681946.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUOY_001109"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUOY_001109"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR0681947.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUOY_001110"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUOY_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR0681948.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUOY_001111"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUOY_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR0681949.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUOY_001112"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUOY_001112"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR0681950.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUOY_001113"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUOY_001113"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR0681951.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="ACHUOY_001114"
     CDS             complement(14275..16539)
                     /locus_tag="ACHUOY_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFR0681952.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHUOY_001115"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHUOY_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR0681953.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHUOY_001116"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHUOY_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR0681954.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHUOY_001117"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHUOY_001117"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR0681955.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHUOY_001118"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHUOY_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR0681956.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5732 c         5044 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacacc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAZOYL010000041        20570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZOYL010000041 DAZOYL010000000
VERSION     DAZOYL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.041"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUOY_001119"
     CDS             complement(<1..215)
                     /locus_tag="ACHUOY_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0681957.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUOY_001120"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUOY_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR0681958.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUOY_001121"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUOY_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR0681959.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUOY_001122"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUOY_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR0681960.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUOY_001123"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUOY_001123"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR0681961.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUOY_001124"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUOY_001124"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR0681962.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUOY_001125"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUOY_001125"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR0681963.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHUOY_001126"
     CDS             complement(4604..5326)
                     /locus_tag="ACHUOY_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0681964.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUOY_001127"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUOY_001127"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0681965.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHUOY_001128"
     CDS             6526..6744
                     /locus_tag="ACHUOY_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR0681966.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUOY_001129"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUOY_001129"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0681967.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHUOY_001130"
     CDS             complement(7478..7678)
                     /locus_tag="ACHUOY_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR0681968.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUOY_001131"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUOY_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR0681969.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUOY_001132"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUOY_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR0681970.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHUOY_001133"
     CDS             10175..12088
                     /locus_tag="ACHUOY_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0681971.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHUOY_001134"
     CDS             12088..13110
                     /locus_tag="ACHUOY_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0681972.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHUOY_001135"
     CDS             13104..13322
                     /locus_tag="ACHUOY_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR0681973.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHUOY_001136"
     CDS             13376..14245
                     /locus_tag="ACHUOY_001136"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR0681974.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHUOY_001137"
     CDS             complement(14300..14704)
                     /locus_tag="ACHUOY_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR0681975.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHUOY_001138"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHUOY_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR0681976.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="ACHUOY_001139"
     CDS             15689..17779
                     /locus_tag="ACHUOY_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR0681977.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUOY_001140"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUOY_001140"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR0681978.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUOY_001141"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUOY_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR0681979.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHUOY_001142"
     CDS             complement(19747..20349)
                     /locus_tag="ACHUOY_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR0681980.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHUOY_001143"
     CDS             complement(20339..20506)
                     /locus_tag="ACHUOY_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR0681981.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
BASE COUNT         5040 a         5636 c         5099 g         4795 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg
//
LOCUS       DAZOYL010000042        20426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZOYL010000042 DAZOYL010000000
VERSION     DAZOYL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.042"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1133
                     /gene="ypdA"
                     /locus_tag="ACHUOY_001144"
     CDS             <1..1133
                     /gene="ypdA"
                     /locus_tag="ACHUOY_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR0681982.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1148..1882
                     /gene="ypdB"
                     /locus_tag="ACHUOY_001145"
     CDS             1148..1882
                     /gene="ypdB"
                     /locus_tag="ACHUOY_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR0681983.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1895..2752
                     /locus_tag="ACHUOY_001146"
     CDS             1895..2752
                     /locus_tag="ACHUOY_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0681984.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="ACHUOY_001147"
                     /pseudo
     CDS             complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="ACHUOY_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="ACHUOY_001148"
     CDS             complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="ACHUOY_001148"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0681985.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="ACHUOY_001149"
                     /pseudo
     CDS             complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="ACHUOY_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7412..8659)
                     /locus_tag="ACHUOY_001150"
     CDS             complement(7412..8659)
                     /locus_tag="ACHUOY_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR0681986.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8681..9007)
                     /locus_tag="ACHUOY_001151"
     CDS             complement(8681..9007)
                     /locus_tag="ACHUOY_001151"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR0681987.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9227..10192)
                     /gene="glk"
                     /locus_tag="ACHUOY_001152"
     CDS             complement(9227..10192)
                     /gene="glk"
                     /locus_tag="ACHUOY_001152"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR0681988.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10396..11652
                     /locus_tag="ACHUOY_001153"
     CDS             10396..11652
                     /locus_tag="ACHUOY_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR0681989.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11767..12093
                     /gene="ypeC"
                     /locus_tag="ACHUOY_001154"
     CDS             11767..12093
                     /gene="ypeC"
                     /locus_tag="ACHUOY_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR0681990.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12234..13472)
                     /locus_tag="ACHUOY_001155"
     CDS             complement(12234..13472)
                     /locus_tag="ACHUOY_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR0681991.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13808..15010
                     /gene="nupC"
                     /locus_tag="ACHUOY_001156"
     CDS             13808..15010
                     /gene="nupC"
                     /locus_tag="ACHUOY_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR0681992.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15060..17248)
                     /locus_tag="ACHUOY_001157"
                     /pseudo
     CDS             complement(15060..17248)
                     /locus_tag="ACHUOY_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(17457..17532)
                     /locus_tag="ACHUOY_001158"
     tRNA            complement(17457..17532)
                     /locus_tag="ACHUOY_001158"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17497..17499),aa:Ala,seq:ggc)
     gene            17753..18112
                     /locus_tag="ACHUOY_001159"
     CDS             17753..18112
                     /locus_tag="ACHUOY_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0681993.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18114..18506
                     /locus_tag="ACHUOY_001160"
     CDS             18114..18506
                     /locus_tag="ACHUOY_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0681994.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18558..19973)
                     /gene="gltX"
                     /locus_tag="ACHUOY_001161"
     CDS             complement(18558..19973)
                     /gene="gltX"
                     /locus_tag="ACHUOY_001161"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR0681995.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20232..20307
                     /locus_tag="ACHUOY_001162"
     tRNA            20232..20307
                     /locus_tag="ACHUOY_001162"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20265..20267,aa:Val,seq:tac)
     gene            20352..20424
                     /locus_tag="ACHUOY_001163"
     tRNA            20352..20424
                     /locus_tag="ACHUOY_001163"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20385..20387,aa:Val,seq:tac)
BASE COUNT         5075 a         5450 c         5079 g         4822 t
ORIGIN      
        1 ttctggtgca aagcgttgag ggcgaaaaag aggccagcgc cgcgcggcag accaagctgg
       61 cgctggatat cgccaacaaa acgctaccgc tgtttcgcca tgtcaatagc gagtcattac
      121 gcaaggtctg cgaaattatc cgcgatgaca ttcacgccga tgcggtggcg attactaata
      181 ccgatcatgt gctggcctat gttggcgtgg gtgaacataa ctatcagaat ggcgatgact
      241 tcattagccc gactacccgt caggcgatga attacggaaa aatcatcatt aaaaacaatg
      301 atgaagccca ccgcacacca gagattcatt ccatgctggt gatcccattg tgggagaaag
      361 gggtcgtgac cggaacgctg aaaatttact actgccacgc gcatcagatc acctcgtcat
      421 tacaggaaat ggcggtcggt ctgtcgcaaa tcatctccac gcaactggag gtttcacgcg
      481 ccgagcagct acgtgaaatg gcaaataagg cagagcttcg cgccctgcaa agcaaaatta
      541 atccccattt tctgtttaac gctctgaacg ctatttcatc gtcaatccgt ctgaatccgg
      601 ataccgctcg ccagttgatc tttaatctgt cgcgttatct gcgctataac attgaattaa
      661 aagacgatga gcaaatcgat atcaaaaaag agctgtatca aattaaagac tatattgcca
      721 ttgagcaggc ccgctttggt gacaagctga cggttatcta tgatattgat gaagaggtga
      781 attgctgcat tcccagcctg ctgatccagc cgttggtgga gaacgccatt gtccacggta
      841 ttcagccttg caaaggtaaa ggcgttgtca ccatcagcgt tgcagagtgc ggaaatcggg
      901 tacgcattgc ggtgcgagat accgggcacg gcatcgatcc aaaggtgatt gagcgggtcg
      961 aagcgaatga aatgccgggc aataaaattg gcctgctgaa tgtccatcat cgcgtgaagt
     1021 tattgtatgg cgaggggctg catatccgcc gcctggagcc ggggacggaa attgcgtttt
     1081 acattcctaa ccaacgcacc ccagtcgcct cacaggctac gttattgctt tgagccggag
     1141 tgatattgtg aaagtcatca ttgttgaaga cgaattcctg gcacaacagg aactgagctg
     1201 gctaattaaa gagcacagcc agatggagat tgtcggcacc tttgacgacg gtctggacgt
     1261 gttgaagttt ttgcagcata accgcgtcga cgccattttt ctggatatca atattccgtc
     1321 gctggatggc gtgttgctgg cgcaaaacat cagccagttc gcccataaac cgtttattgt
     1381 gttcatcacc gcgtggaaag aacatgcggt agaagcgttt gaactggagg cgtttgacta
     1441 cattctcaaa ccgtatcagg agtcacgtat taccgggatg ctgcaaaaac tggaagcggc
     1501 ctggcaacaa cagcagacca gcagtacgcc tgccgcgacg gtaacgcgtg agaatgacac
     1561 cattaatctg gtgaaagatg agcgaataat cgtcacgcca attaacgata tctattacgc
     1621 cgaagcgcac gagaaaatga cctttgtcta tacgcggcgt gaatcctacg taatgccgat
     1681 gaacattacc gaattttgca gcaaactgcc gccgtcgcat tttttccgct gccatcgctc
     1741 attttgtgtc aatctgaaca aaatacgcga aatcgaaccg tggtttaata acacctacat
     1801 tctgcgactg aaagatctgg attttgaagt gccggtcagc cgcagcaaag tgaaagaatt
     1861 tcgccagtta atgcatcttt aatcgggaac tttcatgaaa gcaccaggct tgcccgccga
     1921 tcaacaattt ttcgccgatc tgttcagcgg cctggtgctt aacccgcaac tactggggcg
     1981 cgtctggttt gccagccagc ctgcctcgtt accggtgggc agtttatgta ttgattttcc
     2041 ccgtctggat atcgtgctgc gcggcgaata cggcaacctg ctggaagcaa agcagcagcg
     2101 aatggtggaa ggagaaatgc tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa
     2161 caaaccggtg atgctgttaa gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta
     2221 cgatagccgc accacgtcgt tgttgcatcc tgctcgccag atccagcttc ccagcctgca
     2281 acgcggtgaa ggtgaagcga tgcttaccgc cctcacccat cttagccgtt cgccgctgga
     2341 gcagaatatc attcagccac tggtgttaag tttgctgcat ctttgccgca acgtggtgaa
     2401 tatgccgccg ggtaattcgc aaccgcgcgg cgattttctc tatcacagca tttgtaactg
     2461 ggttcaggat aattatgccc agccgctcac ccgcgagagc gtggcgcagt tttttaatat
     2521 cacgcccaat catctgtcaa aactgtttgc tcagcatgga acgatgcgtt ttatcgagta
     2581 tgtgcgttgg gtgcgaatgg cgaaggcgag gatgattttg cagaaatatc atctgtcaat
     2641 tcatgaagtg gcacagcgtt gcggttttcc ggatagcgac tatttttgtc gcgttttccg
     2701 gcgtcagttt ggtctgacgc cgggagagta cagcgcccgt tttcagggct aacgtcagaa
     2761 ggttaattct gtttccagca gcgtcaggat actttgtgca tcttgcgcgg caaacaacga
     2821 ctggcggaag tttttattca ccagtttacg cgccagctgc gagaagactt tcacatgatt
     2881 catcccttcg ttagcaccca gcgtcagcat gatcaccagt tcaacttcgc ccatttctga
     2941 ctgccagtca accggtttcg ccagccgggc aatgctgata ctggaatgac ggatccactg
     3001 agatttggtg tgcgggatcg ctacgccaaa accaacgccg gtggtaacaa tctcttcccg
     3061 ctgccagaca tcttcttcca gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt
     3121 gccgcacagg aactggatcg cctgctcttt attgctaaaa tcctgatcaa caaagatatt
     3181 ctccagcgcc agcagtgggc gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc
     3241 aatttcctgc gcactgcggc attcacatgc ctgacgcgcc agttcccgac acgcctcgct
     3301 atccagttga cgaagctggc ttttcaccgc cggaatacgc gggctactca tactcagctc
     3361 atccaggccc agcccaagca gtagcggcag ataacggctt tcaccgccca gttcaccgca
     3421 aatgcctacc catttgcccc gctgatgcgc agtggtaact atttgctgca acatgcgcag
     3481 gaacgatggc gtaatcgggt tatatagcgg cgatacgcgc gggttattac gatcgaccgc
     3541 atacagatac tgggtcatat cgttggagcc gatactgaag aaatcgacct catcgcagaa
     3601 gtggtcgatg atgtagcaca ccgacggcac ttccaccatg atcccaagcg taatcgtctc
     3661 tgcatgacgc aggccatcgc gcttaagctc aacgatcgct ttttggatct cgcctttcac
     3721 ccataagatc tgatcgaggc tgttaaccat cgggatcatc aactgggcgt tgccgaaact
     3781 ggcggcgcgc aaaatggccc gcagttgagt gcggaacagg ccagcaaatt ccgggtaaat
     3841 acgtaccgcg cgatagccga ggaacgggtt ctcttcctgg ggaatattca gataaggaat
     3901 gcttttatcg ccgccgatgt ccatcgtgcg gaagataatc ggcttgtcgc ccgccgccag
     3961 tagcacctgc tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat
     4021 atagagcatt tcggtacgga acagcccgac accttccgcg ccgttggcaa acgcgcctgg
     4081 cgcttccaga gcggtgccga tattcgccgc aatatcaata cgcttgttat cacgggaata
     4141 ggccagctgc gcggcagcct gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc
     4201 gacctgataa taaccgctca ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc
     4261 gtcaagcact gcaggttgcc cggcataacg ggcaatcgca tccagcggca agccactcag
     4321 taccgggatc gccgaggcac gggccagaat cagtgtatgc gaggtgcgcc cggttttctc
     4381 cagaatcatg cccgcaagat ttttcaaatc aaggctcaaa aactggcttg gggttaaatc
     4441 ttcagccacc agaatggtcg gtttttccag caccagattg ttgcgcggct tcagttccgg
     4501 ctaggtgata tgcagcaact gttcgctgat atcgcgaatg tcgctgaccc gctcgcgcag
     4561 ataatcgctg gcagaagcag atagtttggc gcaaacctgc tccatattgc tgatgatcgc
     4621 cgcccccagt ccctgatgct gttctgtcat caggcgacgg atattgcctg caaattcatc
     4681 atcctgaatc agcgacaaat gagcgctgag gatagttttg ctttcgccgt cacgctcacg
     4741 caattgctgg ttcagttgct cggcaagcgt tgccaggctg tgctccagcc gggtggaatc
     4801 ttgcgcactg gcggggattg cccgataact gtcgaggctg tcgctttgta acagggtcag
     4861 cgtacccacg ccgacgccgc ttgccagcac attgccgtac agtaaatccg ggttaaggcg
     4921 gcttaatgaa cgcggcagcg gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc
     4981 gctgtcgata aagcgcacca ggatgtactc ttccagcacg cgccgcgcct gctcttcatc
     5041 gctgccgcta atgttcaggc tgcaactgtc attaaataag gtgccagttc caatcaacgc
     5101 cagcgagctt ttagcatcgg cctttgcgtt ctggcgatgg ttgataaagg tgatttcgct
     5161 ttgccactgg ctgcactgtt ctttaagttc ccacgccgga cgagcatgta gaccgttagg
     5221 caaaggacag agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct
     5281 gaaatccgtc agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag
     5341 ttgctccatc tgcaaaatgt cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg
     5401 ggtggcaggc cccattacca ctgtgggtac gccagtatcg gttaagtgca ccgctccacc
     5461 gtccgtgccg ccgttactga acatatccgc ctgcaacgga acgccaattt ctgccgacac
     5521 ggtttcaatc cacgcggtaa gttttggcgg cgcaatcagc gatttatcgc ttaacaccag
     5581 catcggcccg ttaccaatct gacgatggtt agccgcgcca taatcaaagt ttttcgccca
     5641 gcaggcggta tcaagcacaa tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc
     5701 gccgcgtaat cccacctctt cgctggaact tgccaccagc cacacttccg caggcagttc
     5761 ggcgtcgtgc agctcacgta gtaacgtcac cagcagatag caaccgaggc ggtcatcaaa
     5821 ggctttcccc atcacccgct ggtgagggag aacctgaaaa gctgtatcaa aggtgacgcg
     5881 atcgcctgga cgaattcccg cctgcatcac ttcgtcatac gagcgcgcac caatgtccac
     5941 gcgcatggcg ctgacgtcat tcccctgccg gtcgccgtca agcaggcctg gaattttgca
     6001 ctcttcacgg gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gcacgttgcc
     6061 aaccggcagc acatcaattg ccccttcgcg ggagatgctg cgcaccataa atcccacttc
     6121 gtccatatgc gcacagatca tcaccttcgg acctgtcgat tcattgaggc ggatcagcac
     6181 cgatcccaga ccatcaaatc gtacttcttt ttgcaggcga tccgcttctt ccagtaggat
     6241 ctgccgcact tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag
     6301 cgataaatcc attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact
     6361 tctgcgcctt gcggggtaac cagcacaaca tcttcgatgc gcacgccccc ttgccctggc
     6421 aaataaatcc ccggctccac ggtcagtaac atgcctggct gtagcgtcgt ggtatcccgc
     6481 ggtgaaaagc gtggatcttc gtgtacttca ataccgatag catgaccggt gttgtgacca
     6541 aaatagtcgc caaatcctgc ctcggtaatc acccggcgcg cggcgtcgtc aacctgttag
     6601 cagcgtacgc cagggcgaat cgcggagatt gccgccagct gtgcttgcag gacaatctga
     6661 tagacgttaa acagcgggtg agattcggca attacccctt cgccattcac cagcaaggtg
     6721 cgcgtcatat cagagcagta cccctgatag agcgcaccaa aatcaagagt gacaaactcg
     6781 cctgctgcaa caatcttgtc gctggctttg ccgtgcggca gcgccccacg ccagccgctg
     6841 gcgacaatgg tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa ccactccagt
     6901 tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct
     6961 ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt
     7021 tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg
     7081 tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg
     7141 gcaatgattt gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg
     7201 tagccttgcg cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca
     7261 ctttcacggc taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg
     7321 ttctgccgtg aggagagaag cactgcatcc agttgttgcg ccttaagcca gtcgcgcagc
     7381 gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac aatttacctt
     7441 tacggaacat catcagacgc aggaacacca ccatcagcgc agttatgact gcccccagcg
     7501 cgatccccgc catatagacg cccaggttag tgaccagcgg ccatgcccag atagcggatt
     7561 ccgggaacca ttgcactgcg cccagccaga cagcggcggt tgagccgaca atcgcgccga
     7621 ccatatacga cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac
     7681 taatccccat aaaggcaagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga
     7741 acaggcgttt gccggttaaa cgacggtcga taatggtcgc cagacccaga cctatcggcg
     7801 gaataacgat agcgatagaa cgtgcagtga ccggtaatac gtggtcagtg gtaaagctga
     7861 aggcaacaaa acctgcggct ttgttgatcg ggccaccaag gtcgattgcg gtcgccgcgg
     7921 cgatccccat tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc acggtacgga
     7981 taccgccgtt gatccagccg ccaaaggggg taatgacgta gtacatcgcc agcatgacaa
     8041 aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta
     8101 actggatttt ctggttcatc cacttcacaa ggtaacccgc gacgatagaa ataatcagcg
     8161 caccaatgaa ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga
     8221 agttcagcag ttgcgtcggc tgggtagaca tcaagccgcc gataaaacca gccgggaacg
     8281 ccagtttgcc accgatagag ttcgccacaa aagcagcaaa catcggaatg gcaaaaccaa
     8341 acaacacgcc gccgaaggac tgcgacagcc aggcaaattt cagtaaggag aggtcgaaac
     8401 cggagaattt cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc
     8461 agctataagc aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca
     8521 ccatgcgtga aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag
     8581 aggcctgcgg attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac
     8641 tgcgtttttt aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa
     8701 tcatctcttc gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga
     8761 tttcataaac gtcgcgtgat tcgaaacgct cattatcttc cggggtaact gccacggagt
     8821 ggatgatgat ggttgcttcg gcgatatcct gcgccgtcag gcgattctgg ataccgtccg
     8881 cgccctgggt ttcaattttc acttcataac ctgcttctac cgccgcttct tccagcgcct
     8941 gagcggccat aaaggtgtga gccaggccca ttgggcaggc acataaggca attagtttct
     9001 tactcatcgt taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa
     9061 caaatcgccg ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa
     9121 gtgtgacgca ggtcggcctt gtgaggggga cacaaagaaa aattgaatta actgtatgat
     9181 ttaaaagatt atcgggagag ttacctcccg atataacaga aaggatttac agaatgtgcc
     9241 ctaaggtctg gcgtaaatgt gcaccggaac cgagaagacc cggattgtca tggacgatga
     9301 gatacaccgg aatatcatgg acatattctt taaagcgccc tttatcttca aatgcggcac
     9361 ggaaaccaga ggctttgaag aactcaagga agcgcggcac gataccgcca gcaatataaa
     9421 ccccaccaaa ggttcccagc gttaatgcca gattgccgcc aaaacggccc ataatgacgc
     9481 aaaacagcga caacgcacgg cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa
     9541 tatcttttgg cttgagattt tctggcaggc ggttgtcggc tttaacaatt gcgcgataca
     9601 aattcaccag cccaggacca gaaagcacgc gttccgccga aacatgacca atttccgcac
     9661 gcaatatttc gaggataatg gcctcttctt cactattcgg cgcaaaatca acgtgaccgc
     9721 cttcgcccgg caagcttacc caacgcttat cgacatggac cagatgcgca accccaagcc
     9781 ctgttccggc accgtaaacc gcaataggtt taccttcgac cggttctgcg ccaccaaact
     9841 gaatcagatg ctcttttttc agcatcggga tcgccatcga tacagcggta aaatcgttaa
     9901 taatttccag atggctaaaa ccgagattct ttttcatttc ggcaattgag aacgcccagg
     9961 tatggttggt cattgccacc cagtcaccgg taattgggca agcgatggca atacagccgt
    10021 ctttcacctc gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg aggctggggt
    10081 aatcaagccc tgaataggtc ttagcctgcg agatttcacc actggcaata tcacacagag
    10141 caagacgtgc gttggtgccg cccacatcac cgactaatgc atactttgtc attcttcaac
    10201 tgctccgcta aagtcaaaat aattctttct cacactgtaa atacctgggg gcataacaac
    10261 aacgccggaa aggcaggctc cctgtaaata tcgatctggg tcacacattt actttatcgt
    10321 ttcagcacca attgcagcga tgcctttttg caagctgggc aaactaagta tctgaccccg
    10381 cataaggaat agaacatgct ccatccgcga gccagaacca tgttgttatt atcgctcccc
    10441 gccgtggcaa ttgggattgc gtccagtctt attctgattg tggtgatgaa aatcgcctcg
    10501 gtattacaga atttgctctg gcaacgactg ccgggaactc tggggatagc ccaggattca
    10561 cctatctgga tcatcggtgt attaacgcta acgggtattg cggtggggtt ggttatccgt
    10621 ttcagccagg gtcatgccgg accagacccc gcctgtgaac cgctgatcgg cgcaccagtt
    10681 ccgccctctg cgctaccagg acttatcgta gcattaattc tcggtcttgc tggcggcgtc
    10741 agcctggggc cggaacatcc gatcatgacc gtcaatatcg cccttgccgt cgccattggc
    10801 gctcgtctgt taccgcgcgt caaccgaatg gagtggacta ttttagcctc tgccggaacc
    10861 atcggcgcgc tgtttggtac acctgttgcg gcggcgttga tattttcgca aaccttaaat
    10921 ggcagtagtg aagttccgct atgggatcgt ctttttgcgc cgttaatggc ggcagcagct
    10981 ggtgcactta ctaccggatt atttttccat ccccattttt cactgcccat tgcgcattac
    11041 ggacagatgg aaatgaccga tattctcagc ggtgcaattg tcgcggcgat tgccatcgca
    11101 gcagggatgg tcgccgtatg gtgcttacca cggttgcacg cgatgatgaa tcaaatgaaa
    11161 aatccggtgc tcgtgctggg tattggcgga tttattctcg gaattctggg ggttattggt
    11221 ggaccagttt cgctgtttaa agggctggat gagatgcagc agatggtggc aaatcaggct
    11281 ttcagcacca gcgattactt tttgctggcg gtaattaaac ttgccgccct ggtcgttgct
    11341 gccgccagtg gctttcgcgg tgggcgaatc ttcccggcag tgtttgtcgg cgtggcatta
    11401 gggttgatgc tgcatgagca cgttcccgcc gttccagcgg caataaccgt ttcctgcgct
    11461 attctcggca tcgtgctggt ggtaacacgc gatggctggt taagtctttt tatggcggca
    11521 gtcgttgtac ccaataccac attgctaccg ctgctctgta tcgtcatgct tccggcatgg
    11581 ctgttattag caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca gccaccccac
    11641 gataatgttt agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg
    11701 gctttacctc cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga
    11761 ataacaatgt tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca
    11821 gcaaatgcag gtgaaatcac cctactgcca tcgatcaaat tacaaattgg cgatcgcgat
    11881 cattacggta attactggga cggtggtcac tggcgagatc gtgattactg gcatcgcaat
    11941 tatgaatggc gcaaaaaccg ctggtggcgt catgataatg gctatcatcg tggctgggat
    12001 aagcgtaaag catatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc
    12061 ggaaaaggcc gcggacatgg gcaccgccat taaacgcgtc ctccaatgga gcacaatgcc
    12121 tgatgcgacg ctagcgcgtc ttatcaggcc tacaaaccgg ctctttaacg cgtagggcgg
    12181 ataaggcgtt tacgccgcat ccgccaatag tgccagatgc gacgctcatt caactacaat
    12241 cccagcgccg tccccaccaa caaccagata ttcagcgcca cgaccagcac tacaatcacc
    12301 cagcctgtct gttttacgcg tttgctgttc accagatcgc ccatcaactt gctgtcactg
    12361 gtgaaaatca gcagtggaac cagcgccagg gcgataccaa aacttaacag cacctgactc
    12421 ataaccagaa tccgtgtcgg atctaatccc atcagaatga caataaatga cggcaacatg
    12481 gtgactgtac gacgcaccca cagcgggata tggaagcgaa tgaatccctg catcaccacc
    12541 tgccccgcca gtgtccccac caccgttgag gacagtccgg cagcaaccag acttaaccca
    12601 aagaccgttg ccgcagcatg gcttaacagc ggttgcagcg tcagataagc ctcatcaaga
    12661 tcggcaacac cagtatgacc agaaaagtgg aacgccgccg cagctgtagc catcatcgcc
    12721 agattgacaa aaccggcaat cgtcatggcg atagccacat cccatttggt ggcggaataa
    12781 cgttgttgac gcgaaccgcc atgtaaatgc tgagtgagcg aagagtgcaa ataaatcaca
    12841 tgcggcataa tcgtcgcccc taacacgcct gctgccagga agaccgcctc cgaagtaggt
    12901 aaactcggga tcaccattcc tttacccagc tgcgccaggt taggctggga gaaaatcaac
    12961 tcgacaatgt aagccgcggc aacaaacaac agtaacccgc caatcacttt ctccagtggt
    13021 ttttgcccgc gacgttgcag cattaaaatc aggaaagtcg cgatccccgt cagcaccgcg
    13081 ccctgcaaca acgagacacc aagaatgagt ttaaaaccga tcgccgcacc aataaattcc
    13141 gccaggtcgg ttgccatcgc aataatttct gcctgaaccc aatagaacca cactacggga
    13201 cgcggatagt gatcacgaat ctgctccgcc agattttttc cggtggcaat ccctagtttg
    13261 gcagagagga tctgaatcag catcgccatc aggttggccc aaacgacaac ccacagtagc
    13321 tgatagccga agctggcacc cgcctgaata ttggtcgcaa agttaccggg atcgatataa
    13381 ccaatcgccg caatgaacgc aggtcccatt aatgcgagcc tcatcttgcg cgccgcccgt
    13441 ccgctgctac tctcaacgcg atagttcgtc atcttgtgcc tctaaaacat agcctttgct
    13501 atgtttcatg ctatgataaa cgagaatgat tatcaaattc atttaaatgg attgtggtga
    13561 tttctctgat agaccaggat tatgactacg aaaagattga tgacttgaat gttatggtta
    13621 tgtttaatgt tagcacattt acataacttt cagcttccat acacaacata gcagaaatgt
    13681 atgacagatc actatttttg aagcctgtca caggacgtca ttatagtgtg tgtcagatct
    13741 cgttttcctt aaccatgtta catagaatgt gcacggaaat ttaacctgcc tcatatttgg
    13801 agcaaatatg gaccgcgtcc ttcattttgt actggcactt gccgttgttg cgattctcgc
    13861 actgctggta agcagcgacc gcaaaaaaat tcgtatccgt tatgttattc aactgcttgt
    13921 tatcgaagtg ttactggcgt ggttcttcct gaactccgac gttggtttag gcttcgtgaa
    13981 aggcttctcc gaaatgttcg aaaaactgct cagatttgcc aacgaaggga ctaacttcgt
    14041 ctttggtagc atgaatgatc aaggcctggc attcttcttc ctgaaagtgc tgtgcccaat
    14101 cgtctttatc tctgcactga tcggtattct ccagcacatt cgcgtgttgc cggtgattat
    14161 ccgcgcaatt ggtttcctgc tctccaaagt caacggtatg ggcaaactgg aatcctttaa
    14221 cgccgtcagc tccctgattc tgggtcagtc tgaaaacttt attgcctata aagatatcct
    14281 cggcaaaatc tcccgtaatc gtatgtacac catggctgcc acggcaatgt ccaccgtgtc
    14341 gatgtccatc gttggtgcat acatgaccat gctggaaccg aaatacgtcg ttgctgcgct
    14401 ggtactgaac atgttcagca cctttatcgt gctgtcgctg atcaatcctt accgtgttga
    14461 tgccagtgaa gaaaacatcc agatgtccaa cctgcacgaa ggtcagagct tcttcgaaat
    14521 gctgggtgaa tacattctgg caggtttcaa agttgccatt atcgttgccg cgatgctgat
    14581 cggctttatc gccctgatcg ccgcgctgaa cgcactgttt gccaccgtta ctggctggtt
    14641 tggctacagc atctccttcc agggcatcct gggctacatc ttctatccga ttgcatgggt
    14701 gatgggtgtt ccttccagtg aagcactgca agtgggcagt atcatggcga ccaaactggt
    14761 ttccaacgag ttcgttgcga tgatggatct gcagaaaatt gcttccacgc tctctccgcg
    14821 tgctgaaggc atcatctctg tgttcctggt ttccttcgct aacttctctt caatcgggat
    14881 tatcgcaggt gcagttaaag gcctgaatga agagcaaggt aacgtggttt ctcgcttcgg
    14941 tctgaaactg gtttacggct ctaccctggt gagtgtgctg tctgcgtcaa tcgcagcact
    15001 ggtgctgtaa aaccatacat aaaaaagccg gggatcattc cccggctttt ttacgcctgt
    15061 taatcagcta atggctgcgg gcgaccaatc aaataccctt gcagatattg caccccgagc
    15121 ttatgcaata gcgcctgctg ctgttgcgtc tcgacaaact ccgcgaccac actcaatgac
    15181 ttcgctttcg ccagatcggt aattgatctc acaatcatcg catccagcgt attcgtgaca
    15241 atatctttca caaagacgcc atcaattttg atgatatcag cctgcaaacg ctttaaccgt
    15301 tcgtagttgg catatccggt gccaaagtca tcaatcgcaa tccggaaacc aaacttatgc
    15361 agctgctcga tgttgtacat gctgctttct gcgttagaaa acgcctgctc ctcggtgatc
    15421 tcaagaatga ccgcctgcgg ggagatgtga taacgcttaa acagacgaat aatccgcccg
    15481 gcaatattct tttgcagcag cgtgagcggc attaaattga ctgaaaagcg cggtcctttt
    15541 ttgtcgcaag ggtgtgttgc cagccacttc aacaaggatt ccagcacttg caaatcaaaa
    15601 cgcgcgctaa ggttaaactg agcaataagg ggcagaaact tatccggggt cataatgccg
    15661 ccgtcatatt tcagtcgcgc gaggatctca tcataacctt caccctcttt gttgcgaatt
    15721 ggctgggcgt agagcagcaa atcaccctga tctaacgccg tgcgaatggt attcagcaat
    15781 agcacctgtt tagtggtctg cccggaaacc atctcctctc tgctatccag cgccagcaca
    15841 tgatgatgtg cgcaggattg ctccgccagc cagcttaact gccccaacaa gggttgcagg
    15901 gtttcctgat ttccatcaaa acgcccccag gcagcaccat agcccatatc cagcccggta
    15961 ttgttccagt gaatttgccg actattcagg atgttaacca tatgctggag tcgcccttcc
    16021 gtttccggcc cgctcagcac taacagcagt tcactacccg gcaattgata taacttttcg
    16081 ttttcctgca tcaacggcag cagcgtacgg taaattgatc ggatacagtg aacgcgcatc
    16141 attaagccgt aatgacgact cataaactca agattatcaa tgcgcaggca gcaaaactct
    16201 tgcccgcctc ttgctccggc gcttgttcca acgcacggaa gttgggcagg agcgttaacg
    16261 gatcggtcag cgcctgcaaa tgccagcggc gattaagcca ttcactgcga tgataaatgc
    16321 gcaccatata gagcaggcaa acgctaaagg aaatcagcac cgcaagaata aatgccagcg
    16381 aatattcggt ttccacccct tgcaaaaagt tctggttgta attcagaagg caaagcgttg
    16441 aaacagccct ggtgagattt aaaaacggat agcgaagctt accgaccccg agggtaaaga
    16501 tgatgaagaa aacgggtacc aggtaaccgg caataaagtc gttttcataa ggtgtgcaca
    16561 acagaagtag aagcacgctt aacgctgcca gccagcttaa ggtaaatgcg cgtttctctt
    16621 tgcccaacga cggcgcgata tccctgcgcc acaatatctg cgcaaagtgg ggacttacaa
    16681 tcatgcgagt gagatagtag aaaagcatgt tgtaaataag cacagcggtg aaaaggctta
    16741 gcaaatcaac gaccgtgaaa atggcatccg catcgccgaa aaaggtggat atctttagcg
    16801 ggaaatcaaa gaagctcccc acaagataca tgctgcattt gatgccgatc ggcgtcacca
    16861 aaccaagcca gaacagacgt tgccagacat atcggctggt caatccataa cgccaacgcg
    16921 tccccagctg ccagcgtaat attgcgcagg cacacaacac gacaaacgtc tggctaccga
    16981 gcatgaccgc agtctgtaaa aaagagagat gaaagttcca cagattggtg caaaacatcc
    17041 cgcataaaac gggaacgaca ccgcgccagc caaagataaa caagattgac agcattacgc
    17101 acagtggcat ccacgccaga aagatataac tggaatggat aatggcaagt ggcgaaataa
    17161 aacgggatag ctgaataagt accacggtga gcgtaaacgc tagtgtgaat atcttgatat
    17221 tttttatcag gttatgctcc acaaacatga gtacactact ctcactgatc ggggttttca
    17281 ggcacacaaa taataagttt attcgcatcg cgaagcaaac agggggccag cctgttgccg
    17341 atttttcatc caatgatgct ttcaccaggc ggcactaacc cggacgtaga ctgtattaca
    17401 aaagcggcaa aaagcagaga caaaaaaccc ccgctttgca gcgagggttg gaaatttggt
    17461 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    17521 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
    17581 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
    17641 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
    17701 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
    17761 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
    17821 taaatgggta cgcaaggagg gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
    17881 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
    17941 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
    18001 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
    18061 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
    18121 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
    18181 ctgttaaccg ttggatcaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
    18241 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
    18301 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
    18361 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
    18421 ttgagcaggg aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
    18481 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
    18541 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
    18601 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
    18661 gactgccccg ccccggttac ggcgacacgc agcggcatac caactttacc catacccact
    18721 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
    18781 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
    18841 tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
    18901 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
    18961 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
    19021 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
    19081 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
    19141 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
    19201 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
    19261 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
    19321 ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg
    19381 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
    19441 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
    19501 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
    19561 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
    19621 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
    19681 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
    19741 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
    19801 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
    19861 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
    19921 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
    19981 ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag tgggcgatat
    20041 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
    20101 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
    20161 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcggct
    20221 ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    20281 gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg tgtagcaaga
    20341 aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    20401 gcggttcgat cccgtcatca cccacc
//
LOCUS       DAZOYL010000043        20274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZOYL010000043 DAZOYL010000000
VERSION     DAZOYL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.043"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(5..1654)
                     /gene="actP"
                     /locus_tag="ACHUOY_001164"
     CDS             complement(5..1654)
                     /gene="actP"
                     /locus_tag="ACHUOY_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR0681996.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1651..1964)
                     /locus_tag="ACHUOY_001165"
                     /pseudo
     CDS             complement(1651..1964)
                     /locus_tag="ACHUOY_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2164..4122)
                     /gene="acs"
                     /locus_tag="ACHUOY_001166"
     CDS             complement(2164..4122)
                     /gene="acs"
                     /locus_tag="ACHUOY_001166"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR0681997.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4516..5952
                     /gene="nrfA"
                     /locus_tag="ACHUOY_001167"
     CDS             4516..5952
                     /gene="nrfA"
                     /locus_tag="ACHUOY_001167"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR0681998.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5997..6563
                     /gene="nrfB"
                     /locus_tag="ACHUOY_001168"
     CDS             5997..6563
                     /gene="nrfB"
                     /locus_tag="ACHUOY_001168"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR0681999.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6560..7231
                     /gene="nrfC"
                     /locus_tag="ACHUOY_001169"
     CDS             6560..7231
                     /gene="nrfC"
                     /locus_tag="ACHUOY_001169"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR0682000.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7228..8184
                     /gene="nrfD"
                     /locus_tag="ACHUOY_001170"
     CDS             7228..8184
                     /gene="nrfD"
                     /locus_tag="ACHUOY_001170"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR0682001.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8204..9922
                     /locus_tag="ACHUOY_001171"
     CDS             8204..9922
                     /locus_tag="ACHUOY_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR0682002.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSHSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9915..10298
                     /gene="nrfF"
                     /locus_tag="ACHUOY_001172"
     CDS             9915..10298
                     /gene="nrfF"
                     /locus_tag="ACHUOY_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR0682003.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10295..10891
                     /gene="nrfG"
                     /locus_tag="ACHUOY_001173"
     CDS             10295..10891
                     /gene="nrfG"
                     /locus_tag="ACHUOY_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR0682004.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11233..12546
                     /gene="gltP"
                     /locus_tag="ACHUOY_001174"
     CDS             11233..12546
                     /gene="gltP"
                     /locus_tag="ACHUOY_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR0682005.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12625..13314)
                     /gene="yjcO"
                     /locus_tag="ACHUOY_001175"
     CDS             complement(12625..13314)
                     /gene="yjcO"
                     /locus_tag="ACHUOY_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR0682006.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13408..15555)
                     /gene="fdhF"
                     /locus_tag="ACHUOY_001176"
     CDS             complement(13408..15555)
                     /gene="fdhF"
                     /locus_tag="ACHUOY_001176"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15136..15138),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR0682007.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15753..17219)
                     /gene="mdtP"
                     /locus_tag="ACHUOY_001177"
     CDS             complement(15753..17219)
                     /gene="mdtP"
                     /locus_tag="ACHUOY_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR0682008.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17216..19267)
                     /gene="mdtO"
                     /locus_tag="ACHUOY_001178"
     CDS             complement(17216..19267)
                     /gene="mdtO"
                     /locus_tag="ACHUOY_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR0682009.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19267..>20274)
                     /gene="mdtN"
                     /locus_tag="ACHUOY_001179"
     CDS             complement(19267..>20274)
                     /gene="mdtN"
                     /locus_tag="ACHUOY_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR0682010.1"
                     /translation="PRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVV
                     PEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVD
                     AQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADL
                     NAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSV
                     GQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSI
                     GYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEP
                     Q"
BASE COUNT         4521 a         5417 c         5567 g         4769 t
ORIGIN      
        1 gagattaatg tgcgcggcct tgctcaacgc caaagccggt ctgggagcgg ataaactgcg
       61 cgcggaacag ttcacgctcg cgcgcccctt ccgctgagtt atcggttgcc gagaagaacc
      121 agatgccgag gaatgccacg gtgatagaga acagcgccgg gtattcatac gggaagatgg
      181 ctttttcgtg accaaggatc tgtacccaaa tcgtcgggcc gaggatcatc agtactactg
      241 cggtaatcag ccccagccag ccacccatca tcgcgccacg cgtggtcagt ttcgaccagt
      301 acatagaaag cagaatgatc gggaagttac agctcgccgc gatggcaaac gccagcccca
      361 ccataaaggc gatgttctga ttctcaaaca gcacgccgag gataatcgca atcacgccga
      421 ggatcagtac ggtgattttt gatacccgca gctcttcacg ttcggtcgcg ccttttttga
      481 agacgttagc gtacaagtca tgcgaaaccg cggatgcgcc cgccagcgtc agacccgcaa
      541 ccaccgcgag gatagtggcg aaagcaaccg ctgaaataaa accgaggaac aggttgccgc
      601 ccactgcatt cgccaggtga acggccgcca tgttgttacc accaatcaga tggcccgccg
      661 cgtctttata ttccggattc gcaccaacca gcatgatcgc gccgaagccg ataataaagg
      721 tcagaatata gaagtagccc ataaacccgg tggcgtagaa cacgctctta cgtgcttcgc
      781 gggcatcgct gactgtaaag aagcgcatca gaatgtgcgg caagcccgcc gtaccaaaca
      841 tcagtcccag acccagagag agtgcggaga tcggatcttt caccagcccg cccggcttca
      901 tgatgtcgac acctttcggg tgtaccgcca tcgcttcact gaacagattg ttgaagctaa
      961 agccgacgtg tttcatcacc ataaaggcca taaagctggc accgaacagc aacagcacgg
     1021 ctttgataat ctgtacccaa gtggtcgcca gcatgccgcc gaacaggacg tacatcatca
     1081 tcagcacgcc gaccagcacc accgcaatgt gatagttaag gccaaacagc agctcgatca
     1141 gtttacctgc gcccaccatc tgggcgataa ggtaaagcgc caccaccacc agagaaccac
     1201 aggccgaaag aatacgaatc ggcccttgtt tcagacggta agaggccaca tcggcaaagg
     1261 tgtagcgccc caggttacgc agacgttcgg caatcaggaa caaaatgatc ggccagccca
     1321 ccaggaagcc cagcgagtaa attaagccgt catagccgga ggtaaacacc agcgcggaga
     1381 tccccaagaa tgaggcggcg gacatatagt ccccggcaat cgccagcccg ttctggaagc
     1441 cagtgatatt gccgcctgcg gtgtagtagt cgctacgaga acgtacgagt tttgatgccc
     1501 agtaggtaat gccgagcgta aacacgacga aaatcaggaa cataataatc gcctgccagt
     1561 tcgttggctg gcgctctacg gccccgctaa tagcatccgc ggcgttagct gcgaaaggga
     1621 gtgtggcggc aagcgccgtc agaactcttt tcatgatgct tgtacctcat gcaggacttc
     1681 attattaaga cggtcgaatt cgccgttcgc ccgccagatg tagataccgg tgagaacaaa
     1741 ggagatcaca atcactccaa caccaattgg aataccgcgt gtgacgctgg tgttcggatt
     1801 cagcggcgtg ccagccagcc gggcgcgaag gcgatcagta aaataaagcc gatataaact
     1861 gccagcataa taatcgacag gatggtggca aaccgttgcc gtttttcgac taactccctg
     1921 aaatgcgcat tgtcttctat ccgctgataa atagtgccat tcatcacaga ttctccagag
     1981 gtaatgtagg gattgtttta attcccgctc ccttatggga gaaggttaac gctcgggtaa
     2041 cccttgccga atgtaggccg gataaggcgt ttacgccgca tccggcaatc aatgcctgat
     2101 gcgacgctgt cgcgtcttat caggcctaca aaccgttacc gactcgcatc cggcaattgt
     2161 gggttacgat ggcatcgcga tagcctgctt ctcttcaagc agcttctcga ctacgccagg
     2221 atcggcaagc gtcgaggtat cgcccaggtt gctggtatcg cccgccgcaa ttttgcgcag
     2281 aatacggcgc ataattttgc cggagcgggt tttaggcagg gagtcggtcc agtgcagcac
     2341 gtctggcgtc gccagcgggc caatctcttt acgcacccag ttgcggactt ctgcgtacag
     2401 ttctggtgac ggttcctccc cgtgattaag cgtgacgtag gcgtagatcg cctgaccttt
     2461 aatattgtgc ggaataccta ctacggcggc ttcggcaatc ttcggatgcg ccaccagcgc
     2521 cgactcaatc tctgccgtcc ccagacggtg accggagacg ttcagcacgt cgtccacacg
     2581 cccggtgatc cagtaatagc catcttcatc gcgacgcgcg ccgtcgccgc tgaaatacat
     2641 atttttgaag gtggagaagt aggtctgttc aaaacgttcg tgatcgccaa acagcgtacg
     2701 cgcctgaccc ggccaggagt cggtgatcac caggctgcct tcggtagcgc cttccagcgg
     2761 gttaccttcg ttatcgacca gcgccggttg cacgccgaag aacggacgtg ttgccgaacc
     2821 ggctttcagc tcggtagcgc caggcagcgg cgtgatcatg aaaccgccag tttcggtctg
     2881 ccaccaggta tcgaccaccg gacatttctc gttgccgatt tttttccagt accactccca
     2941 cgcttccggg ttaattggct cgcccacgga accgagaatg cgcagcgacg aacggtcggt
     3001 accctcgatc gctttatcgc cttccgccat cagcgcgcgg atcgccgtgg gtgcggtata
     3061 gagaatattg acctgatgct tgtccaccac ctgcgccata cgggcaggcg tcggccagtt
     3121 gggtacgcct tcaaacatca gcgtggtcgc accgcaggcc agcgggccgt acagcaagta
     3181 actgtgtccg gtcacccagc ccacatcggc ggtgcaccag tagatatcac ccggatgata
     3241 atcaaagaca tatttaaagg tcagcgccgc gtacaccaga taaccgccgg tagtatgcag
     3301 cacacctttt ggcttaccgg tagaaccgga ggtgtagaga ataaacagcg gatcttcggc
     3361 gttcatcttt tccgcctggt gctgatcgct cgcttgctca accaggtcgt gccaccacag
     3421 gtcgcgccct tcctgccagt caattttccc gccagtacgc ttcagtacca ccacatgctc
     3481 tacgctggtg acgttcgggt ttttcagcgc gtcatcaacg tttttcttca gcggaatact
     3541 gcgcccggca cgcacacctt cgtcggaagt gatcaccagt cgtgagttgg aatcaataat
     3601 gcgcccggca acggcttccg gcgagaagcc gccgaaaatc accgaatgca ccgcgccaat
     3661 gcgggcgcag gccagcatcg caaccgcggc ttccggcacc atcggcatat aaatcgccac
     3721 cacatcacct tttttaatgc ccagctcgag cagggtattg gcgaagcggc agacgtcgcg
     3781 gtgcagctct ttatagctga tatgtttgct ctggctggcg tcgtcgcctt cccagatgat
     3841 ggcggtacga tcgccgtttt cttgcagatg gcggtcaagg cagtttgccg ccagattcag
     3901 cgtgccgtcc tcgtaccatt taatggacac attaccgggg gcaaaggagg tgtttttcac
     3961 cttctggtaa ggtttgatcc agtcaagaat ttttccctgt tcgccccaga aggtatcagg
     4021 tacgttaata gattgttgat acatcgcctc gtactgctga gggtttatca ggcaacggtc
     4081 tgcgatgttg gcaggaatgg tgtgtttgtg aatttggctc atgcttttgt tctccttgta
     4141 gggtgttaat aatatgtggc ataagcgtta aatgtagggg tattggcagt tttgtttagt
     4201 atttgggcga cagatcacgc aaaagtagaa ttgtgcaaat aaacggcagg gtaatttttg
     4261 aaggtcagca acaaaagttg attaattctt tgaggaacat gcagttatgc attgctgttg
     4321 aaaaagagga agatactaac taactctaaa gtggtatttt acatgcactt acaattgatt
     4381 aaagacaaca ttcacagtgt ggttatttgt tacacatagg ggcgagcaat gtcatgacag
     4441 tgtaggtgcg gttactgtcg tgaaaaacaa taaaaaccgc cattgtaaca atggcgcaat
     4501 tcggatgaag cccctatgac aaggataaaa ataaacgcac gccgtatctt cagcttattg
     4561 attccttttt tctttttcac ttctgttcac gctgaacaaa cggcagctcc cgcaaaacct
     4621 gtaactgtgg aagcgaagaa tgaaaccttt gccccgcagc atcccgatca atatctctcc
     4681 tggaaagcca cctcggaaca gtcagagcgt gttgacgccc tggcggaaga tccacggctg
     4741 gtgatcctgt gggcggggta tcccttctcg cgcgattaca acaagccgcg tggacatgcc
     4801 tttgctgtga ccgatgtgcg tgaaaccctg cgtaccggtg cgccgaaaaa cgctgaagat
     4861 ggtccgctac cgatggcgtg ctggagttgt aaaagcccgg atgtggcgcg tctgatccag
     4921 aaagacggcg aagatggcta cttccacggt aagtgggcgc gcggcggccc ggaaatcgtc
     4981 aacaacttag gttgtgccga ctgccataac accgcctcac cagagttcgc caaaggcaaa
     5041 ccggagttaa ccctttcccg tccgtatgcg gctcgcgcga tggaagccat tggtaaacct
     5101 tttgagaaag ccggacgttt cgaccagcaa tcgatggttt gcggtcagtg ccatgtggag
     5161 tattacttcg acggcaaaaa caaagcggtt aaattcccgt gggatgacgg catgaaagtc
     5221 gaaaatatgg agcagtatta cgacaaaatt gccttctctg actggactaa ctccctgtcg
     5281 aaaacgccaa tgctgaaagc gcagcacccg gaatatgaaa cctggacagc gggcattcac
     5341 ggtaaaaaca acgtgacctg tatcgactgc catatgccaa aagtgcagaa cgccgaaggc
     5401 aaactctata ccgaccataa aattggtaat ccgtttgata acttcgccca gacttgtgcg
     5461 aactgccata cccaggacaa agctgccttg caaaaagtgg tcgcggaacg taagcagtcg
     5521 attaacgacc tgaaaatcaa ggttgaagat caactggttc acgctcactt cgaagcgaaa
     5581 gcagcgctgg atgcaggcgc gacggaagct gaaatgaagc caattcagga cgatatccgt
     5641 catgcccagt ggcgctggga tctggcgatc gcttcccacg gcattcatat gcacgcaccg
     5701 gaagaaggtt tacggatgct cggtacggcg atggataaag cggcggatgc acgcaccaaa
     5761 ctggcgcgcc tgctggcgac caaaggcatc acccatgaaa tccagatccc ggatatctca
     5821 accaaagaga aagcccagca ggccattggc ctgaacatgg aacaaatcaa ggccgagaag
     5881 caggacttca tcaaaacggt gatcccgcag tgggaagaac aggcacgtaa aaacggtctg
     5941 ttaagccaat aaccccgttc cgcctcgcaa ggggcggaaa acacaatgga gtgaatatga
     6001 gcgtattacg ttcgttatta actgccgggg tgctggcgtc gggcctgttg tggagcctga
     6061 acggaattac cgctacccct gcggcgcagg catctgatga tcgctacgaa gttacccagc
     6121 agcgtaaccc ggatgccgcc tgtctggact gtcataaacc agataccgaa ggtatgcatg
     6181 gcaaacatgc ttccgttatc aacccgaata acaaactgcc ggtcacctgc accaactgtc
     6241 acggccagcc atcaccgcaa caccgcgaag gggtgaaaga tgtgatgcgc tttaacgagc
     6301 cgatgtacaa ggttggagag cagaacagcg tctgtatgtc ctgtcatctg ccggaacagt
     6361 tgcaaaaagc gttctggccg cacgatgtcc acgtaaccaa agtggcgtgc gccagctgcc
     6421 attccctgca tccacagcaa gatacgatgc agacgttaag tgacaaagga cggattaaga
     6481 tttgcgtcga ttgccacagc gatcagcgca ccaatccgaa ctttaacccg gcgtctgttc
     6541 cgttgcttaa ggagcagcca tgacctggtc tcgtcgccag tttctcaccg gcgtcggcgt
     6601 gctggcagcc gtcagcggca ccgcagggcg cgtggtggcg aaaacgttga atatcaatgg
     6661 ggtgcgttac ggcatggtgc atgacgagtc gttatgcatc ggctgtaccg cctgtatgga
     6721 tgcttgtcgg gaagtgaaca aggtgccgga aggcgtctcg cgcctgacga ttattcgtag
     6781 cgagccgcag ggcgaatttc ctgatgtgaa atatcgcttc ttccgtaagt cttgccagca
     6841 ctgcgatcat gcgccgtgcg ttgacgtctg cccgaccggt gcgtcttttc gtgatgccgc
     6901 tagcgggatt gtcgatgtta atcctgatct ctgcgtcggt tgtcagtact gcatcgccgc
     6961 ctgtccgtac cgcgtgcgct ttatccatcc ggtcacgaag acggcggaca aatgcgattt
     7021 ctgccgtaaa accaatttgc aggccggtaa gctgcccgcg tgtgttgagg cttgcccgac
     7081 gaaggcgctg acgtttggca atctggacga tcctaacagt gagatttcgc aactgctgcg
     7141 ccagaagcct acttaccgct acaagctggc gctgggaacc aaaccgaagc tgtaccgcgt
     7201 accgtttaaa tacggggagg tgagccaatg acgcagactt ccgcatttca ttttgaatcg
     7261 ctggtgtggg actggccgat tgccatctac ctgtttttga ttggtatttc tgccggtctg
     7321 gtgacgctgg ccgtgctgtt acgtcgcttc tacccgcagg cgggcggtgc agacagtacg
     7381 ttgctgcgca ccacgctgat tgtcgggccg ggcgcggtga tcctcggtct gttgatcctc
     7441 gtcttccacc tgacaagacc gtggaccttc tggaagctga tgttccacta cagttttacc
     7501 tcggtgatgt cgatgggggt gatgctgttt cagctctaca tggtggtgct ggtgctgtgg
     7561 ctggcgaaaa tctttgaaca tgatttgctt gccctgcaac aacgctggtt gccgaagctg
     7621 gggatcgtgc aaaaggttct gagcctgctg acgcccgttc atcgcggact ggaaacgctg
     7681 atgctggtgt tggcggtgct gctgggggct tataccggct ttctgctgtc ggcgctgaaa
     7741 tcgtatccgt tcctcaataa cccgatcctg ccggtgctgt tcctcttctc cggcatctcg
     7801 tccggtgcgg cggtggcgct gatcgccatg gcgatacgcc aacgcagtaa cccgcattcc
     7861 acggaagcgc actttgttca ccggatggaa atccccgtgg tatggggtga aatcttcctg
     7921 ctggtggcgt tttttgtcgg tctggcgctg ggcgatgacg gtaaagttcg tgcgctggtg
     7981 gcggcattag gtggcggttt ctggacgtgg tggttctggc ttggtgtcgc cgggctgggg
     8041 ctgattgtgc caatgttgct caaaccgtgg gtcaatcgca gttccggcat tcctgccgtg
     8101 ctggcggcgt gtggggccag tctggtcggc gtgttgatgc tgcgcttttt cattctctac
     8161 gccggacagt tgacggtggc gtaagccaga aaagaggtgg tttctggacg tattccttcc
     8221 tgaagtcggt tttctggcgt tgttgttaag tctcggggtc aacgtgttga ccccgttgac
     8281 ggcctttgcg ggagtgcggt tgcgctggcc tgccatgatg cgactcactt gcatcggcat
     8341 tctggcgcag ttcgcgctcc tgctgctcgc ctttggcgta ctgacgtatt gttttctcat
     8401 cagcgatttc tcggtcattt atgtcgcgca acatagccat agcctgctgt cgtgggaatt
     8461 caagctggca gcggtgtggg gcggccatga aggttcgctg ctgctttggg tgctgctgct
     8521 ttccgcctgg agcgcgctgt ttgcctggca ttatcggcag caaaccgatc cgctatttcc
     8581 gctgacgtta gccgttttat ctctcatgct cgccgcactg ctactgtttg tggtgctgtg
     8641 gtccgatccc ttcgtgcgga tatttccacc agcaatcgaa ggccgcgatc tcaatccgat
     8701 gctgcaacat cccggtctta tctttcatcc accgctgctt taccttggct atggcggttt
     8761 gatggtagcg gcgagcgtgg cgctggcgag tctactgcgc ggcgagtttg atgcggccag
     8821 cgcccgaatt tgctggcgct gggcgttacc tggctggagt gcattaacgg cggggatcat
     8881 cctcggttcc tggtgggcct actgcgaact cggctggggc ggctggtggt tctgggatcc
     8941 ggtggaaaat gcctctttat taccctggct ttctgccact gcgctgctgc acagtttatc
     9001 cctgacacgc cagcggggga ttttttgcca ctggtcgctg ttgctggcga tagttactct
     9061 gatgctgtcg ctgctgggca ccttaattgt ccgttctggc attctggttt cggttcatgc
     9121 gttcgcactg gataacgtcc gcgccgtgcc gttgttcagc ctgtttgcac tgattagcct
     9181 tgcgtctttg gctctgtatg gctggcgagc gcgggacggt ggcccggcgg tgcgtttttc
     9241 ggggttatcg cgggaaatgt taatcctcgc tacgctgttg ctgttttgcg cagtgctact
     9301 gatcgtgctg gtgggaacgc tttatccgat gatttacggt ctgctgggct ggggacgcct
     9361 ctccgttggc gcgccgtatt ttaaccgcgc gacgttaccg tttggtctgt tgatgctggt
     9421 ggtgattgtg ctggcgacgt ttgtctctgg caaacgcgcg cagcttccgg cgctggtcgc
     9481 tcatgcgggc gtgctgttat ttgccgcagg gatcgtggtt tccagcgtca gccgtcagga
     9541 gatcagcctg aatttacagc cgggtcagca ggtgacgctg gcaggataca ccttccgttt
     9601 tgagcgcctc gatctgcaag ccaaaggcaa ttacaccagc gaaaaagcga tagtggcgct
     9661 gtttgaccat cagcaacgca ttggtgaatt aacgccggag cggcgttttt atgaagcacg
     9721 ccgtcagcaa atgatggaac cgtcaattcg ctggaacggc atccatgact ggtatgcggt
     9781 catgggtgaa aaaaccggag cggatcgtta cgcttttcgt ttgtatgtac aaagcggtgt
     9841 gcgctggatc tggggtggag gattgttgat gattgcgggc gcattgttaa gcggatggcg
     9901 ggggaagaag cgcgatgaat aaagggcttc tcacgctact gctgttattt acctgttttg
     9961 cgcgcgctca ggtcgtagac acctggcaat tcgccaatcc gcaacaacag caacaggcgt
    10021 taaatattgc cagccagtta cgttgtccgc agtgccagaa tcaaaactta ctcgaatcca
    10081 atgcgccggt ggcggtcagt atgcgccatc aggtttatag catggtgtcg gaggggaaga
    10141 acgaagtcga aatcattggc tggatgaccg aacgctacgg agattttgtt cgctataacc
    10201 caccgttaac gggtcagacg ctggtgttgt gggcgctgcc agtggtgttg ttattgctga
    10261 tggcactgat cctctggcga gtgagggcta agcgatgaaa cagcctcaaa taccggtgaa
    10321 aatgctgaca acactcacga tattgatggt attcctctgt gtcggcagtt atctgttaag
    10381 cccaaaatgg caggcggtac gtgcggagta tcagcgtcag cgcgatccgc tacatcagtt
    10441 tgccagccag caaaccccgg aagcgcagct tcaggcattg caggataaaa tccgcgctaa
    10501 tccacaaaac agcgaacaat gggcgttact gggcgagtac tatctgtggc aaaacgatta
    10561 cagcaattcg ctgctggcgt atcgtcaggc gttgcaacta cgtggtgaga acgctgaact
    10621 gtatgcggcg ctggcgacgg tgctttatta ccaggccagc cagcatatga ccgcccagac
    10681 ttgcgcaatg atcgacaaag ccctcgcgct ggactctaac gaaatcaccg ctctgatgct
    10741 gctggcttcc gatgcgttta tgcaggcgaa ttacgcgcaa gccatcgaac tatggcaaaa
    10801 agtgatggat ctcaactcac cccggatcaa ccggacacag ctggtcgagt cgattaatat
    10861 ggcaaaactg ttgcagcgga gatcggatta atcatctggt gatgaagatt atgtcattga
    10921 gctgcataaa aaataatcga atgaacatat gccaaaaata atcactaatc agtattattg
    10981 cagattaaac aaataaaaat ctttccataa caaatggtta ttcattaatc ctgccataaa
    11041 ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt gcgcaacata
    11101 ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta caaaccaacg caacacaatt
    11161 catgccctgg cagtatgtca cgttctcgcg tttctgaacg gggaacggcg ctccattgag
    11221 gaagttattc tgatgaaaaa tataaaattc agcctggcct ggcagattct gtttgctatg
    11281 gtgctgggca ttctcctggg aagctacctg cactaccata gcgacagccg cgactggctg
    11341 gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc atctgatcaa aatgattgtt
    11401 gtgccgattg tgatctccac gctggtggtg ggtatcgcgg gtgttggtga tgccaaacag
    11461 ctcgggcgta ttggcgcgaa aaccattatt tacttcgagg tgatcaccac cgtcgccatc
    11521 attttgggga tcactctggc gaacgtcttc cagcccggtg ccggggtgga tatgtcgcag
    11581 ctggcgaccg tcgatatctc gaaatatcag agcactacgg aagcggtaca aagcagttcc
    11641 cacggcatta tgggcacgat tttgtcgctg gtgccgacga acattgtggc gtcgatggcg
    11701 aaaggcgaaa tgctgccgat catctttttc tcggtgctgt ttggtctggg gctttcttcc
    11761 ctgcccgcga cgcatcgtga accgctggtg accgtgttcc gctccatctc tgaaaccatg
    11821 tttaaagtga ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc gctgattgcg
    11881 gtgacggtgg ctaactttgg tttctcttct ctgtggccac tggcgaaact ggtgctgctg
    11941 gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc gcgcctgtgc
    12001 gggttaagcg tctggatcct gattcgtatt ctgaaagatg agctgattct ggcgtactcc
    12061 actgccagct ctgaaagcgt actgccgcga attattgaga agatggaagc ctacggagca
    12121 ccggcgtcga tcaccagctt cgtggtgccg accggttact cttttaacct tgatggttcg
    12181 acgctgtatc aaagtattgc cgctattttc atcgctcagc tgtatggcat tgacctgtcc
    12241 atctggcagg aaatcattct ggtgctgacg ctgatggtga cctcgaaagg gattgctggc
    12301 gtgccgggcg tgtcgtttgt ggtgctgctg gcaacgctgg gtagcgtagg tattccgctg
    12361 gaaggtctgg cgtttattgc tggtgttgac cgtatcctcg acatggcgcg tactgcgctg
    12421 aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca caaatttgac
    12481 cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca aatttgataa aactgcggat
    12541 caataattga agattgccgg ggatatccac ccggcaatgt gtgaatgcct gatgcgacgc
    12601 ttgccgcgtc ttatcaggcc taccttaacc gttggttaat ttttcaaact cttcacaccc
    12661 ggtatcaaac ccttccatac agctcaggtt caaccagtgc agcgcctttt gcttgttctt
    12721 ctcgataaaa ccttcttcac cgtttaagaa catcattccc gcccagtact cggaataacc
    12781 ggttcgggaa attgcagagc tgcgtttgaa ataccaggtt gccttgtcat cgtcggcctt
    12841 aatgcccacg ccgttggcgt aaatcaaacc aagcagcatt tgggcatcga cggcagagtc
    12901 gttctccaga tcttccgagg cgttttctaa cagcgaaata gcctttggat aatccggttt
    12961 acccgcttga gtatttacca gaatatgcgc cagcgttact tcaccttctt tactgcccgc
    13021 ttgcgccgct ttctctgcaa gaacttttgc ttgtggatag tccagactga ccggattggt
    13081 gattttgatc tgcgccagca gcgcgcaggc atcagcatca ccgctgtcgg cggctttctg
    13141 tgcccaatat tcggctttgc tcaaatcgcc ggagctaaac cagctgtcgg caagaaaata
    13201 ttgtgcgcgt cggtccccgg cctctgctgc ctttaaatac tggctgccag gctcggagtc
    13261 gttggcgtgg gcaaagaatg tcaaaaacaa catcaatgcg ataatttttt tcattttgat
    13321 aatacgattg ggtagggagt aaccagtata aagagttcga atggataaaa aaacagcctc
    13381 cgaaaggagg ctgtagaaag gactgtatta cgccagtgcc gcttcgcgca ggcgagtttt
    13441 caacttgttg tactcgtcaa tcacgtactg ctcggcggcg cgctgatcgg cgatcggctc
    13501 gacgcgcacg gcgcagtatt tgtattccgg cgttttcgta atcgggctta agttttcggt
    13561 aaccagctcg ttacaggcac caatccacca ctggtaggtc atgtaaatcg cccctttgtt
    13621 cggacgatcg ctgacctgcg cacgggtgat aattttgcct ttacgcgagt gcacccaaac
    13681 caatgcctca tcttcaatac ccagacgttt ggcgtcttcg gtattgattt gtgcgtagcc
    13741 aggttcatca gccagcgccg ccagtgccgc acagttaccg gtcatcgaac ggcaagagta
    13801 gtgaccaact tcacgcaccg ttgacagtac catcgggtac tcgtcggtga gtttgtcgat
    13861 tggcgctacc cagtcgcagg tgaagaactg cgccagaccg ttcggggtat caaacttctc
    13921 tttaaacaga taagaagtcc cctgatcggc atctgaagta tcgcggcaag gccactgaat
    13981 gaagcccagt tcgcccattt tctcgtaagt cgcaccgtag aaatccgggc acagatgacg
    14041 caactcatcc cagatctcct gggtgttgtt gtagtgcatc ggataaccca tacgggtggc
    14101 gatttcactg atgatttgcc agtccgtttt cagatcccat ttcggttcaa ctgctttgaa
    14161 gaaacgctgg aagccacggt cagccgcagt aaacacgcct tcatgctcgc cccacgacgt
    14221 tgacggcaaa atgacatccg ccgccgacgc ggttttggtc ataaagatgt cctgaacgat
    14281 aaccagttcc agatcttcaa aggctttacg tactgccgac agctccgcgt cagtttgcag
    14341 cggatcttcg cccataatgt acgcggcacg cacttcgcca tgcgctgcgc ggtgcggcag
    14401 ctcgctgatg cgataaccgg tatgagcagg caggctttcc acgccccagg ctttggcgaa
    14461 tttctcgcgg ttagccggat ctttcacgta ctggtatccc ggatacgtat ccggcagcgc
    14521 gcccatatcg caggcaccct gcacgttgtt ctgaccacga accgggttta cacccgcatg
    14581 cggcttaccg aggttaccag tcagcatcgc gaggctggtc agagaacgca cggtttccac
    14641 gccctggtag aactgggtta cacccatgcc ccacaggatg gcggcgcttt tcgcctgggc
    14701 atacatccgt gccgcctgac gaatctcact ggcgctgacg ccggtgatat cttcaaccga
    14761 ctccggcgtg tagccttcaa cgattttacg atactcttca aagccttctg tacgggaagc
    14821 gacgaacgct ttgtcgtaca gattttcttc aataatgaca tggcccatcg cattcaacag
    14881 cgcgatgttc gagccgtttt tcagtgcaat gtgcatgtca gcaatgcgcg cggtttcaat
    14941 tttgcgcgga tcgcagacga taattttcgc cccgttacgt ttagcgttaa ttacgtgatt
    15001 cgccacgatt gggtgggaat cagccgggtt gtacccgaaa acgaacacta aatcggtatt
    15061 atcaatttcg ttaatagcat tgctcattgc gccattaccg accgattggt gcagacctgc
    15121 aaccgatggg ccgtgtcaga cacgagcgca gcagtcaacg ttattggtac caataacggc
    15181 gcgcgcaaat ttttgcatta catagttggt ttcgttaccc gtaccacgcg aggagccggt
    15241 cgtctggatg gcatccggac cgtacttctc tttgatggcg ctcaggcgct cggtaacgta
    15301 attcagtgcc tcatcccagg aaacaggttc gagtttgccg ccacgctggc gacggatcat
    15361 gggggttttc aggcgcgggg tcaggatctg ggtatcgtta atgaaatccc agccataata
    15421 acccttcaga cacagggtac cctggttggt tttcccctgc gctgcctccg cccggacgat
    15481 tttgccgtta tcgacgacca ggttgatttt gcaacctgat gcgcaatagg ggcaaaccgt
    15541 gacgactttt ttcatcggtc tcgctccagt taatcaaatc acgcatacgc gctctcgact
    15601 acagtatgca tcttttatgc cacattttat ttggggtcat tccctgatat tacgggcact
    15661 atttattcaa aactctgacg aaaaacaggc tgtcgtcagt tttgacgtga cgaaacgaaa
    15721 taccgcgtga cagccatcac gcggcagaca ttttattttt tctcgacgac gggagccgcc
    15781 tgatacccgc cgcccagcga tttcatcaac tgaatgcttt ggatcacccg gcggctgtcc
    15841 agcatcagta atgacatctc ttcggcaagt actggcaacc gggcttcagt ggcctgtaag
    15901 cggctggtta agccgcgctg ataggcggcc tcggcagcgc gctgggtgaa gcgcgttgct
    15961 tccacgcgtt cagcctgcat ttctcgctcg tcgttgagcg tttgcagacg cgtgccgttg
    16021 acggcaacgt cacgcaccgc gttcagtact gactggttgt aacgttcaat catcatgttg
    16081 ctggcggcgc gcgtgccttc gagattggca ttcaaccgtc caccgtcaaa cagcggcaat
    16141 ttcagacccg ggatgaagtt gaactggcga ctggtttttt tgaataaggt atccaggtgg
    16201 atggcatcaa ggccgaaaaa cgctttgata tcaaagctcg ggtagaacag cgcccgcgcg
    16261 gaatccacct gatctaatga cgcctgaaca taccagcgca tggcttgcag atccgggcgt
    16321 ctggcgagca actcataaga gagtgtcgcc ggaatgccgg tctggactcg tggtaatgcc
    16381 accggtttga tctccggcat atcgctggcc cccgcgccaa tcaatgcacg cagagattct
    16441 cgcgtttcgg tgatttgccc tttgacggca gcaatttgtt tatcgaccgc cagaatctgt
    16501 gcccgcgcgc cgtggaaagg cacttgtgct tccagaccgt gcgccacttt actctggtgc
    16561 gctttcaccg cgtaatcaat cacatcgcga gtttgttcta acagatcgag catctgatag
    16621 ctggcctgca tactgtaata aagctgcgct acgcccgtgg tcagcgatag ctctactgct
    16681 gcggtttctg ccagcgcggc attatgcgcg ccaatggcgg cggcaaccgc tgagcgatgc
    16741 acaccccaca aatcaagatc cagtccggca aacaaaccta ctgtggcttc cgtatagtac
    16801 ggcccgtcca tacccagtgc gggcgcatcc atcgcataag ggcttaaaaa gccgttcgcc
    16861 gagacgcgtt ggcggttgag catccctaac gctgcgacct gtaattgtga accggcatct
    16921 aacaaatcgg cctgggactg cgctttttct tcccgcagtt tcgcttcggc gagggtgtgt
    16981 gaaccactta gcgtccgttg gatcagcgca tccagctgcg ggtcattgag ttgtttccac
    17041 cactgcgcct gcggccagcc ggagctggca agatgaatat catcggccag tttgatttgt
    17101 tccggtttga gctgttgatg aggcgcagaa tccttgcgta ccaaggcaca gccggaaatc
    17161 agcgtcgtgc tgccgagaat gctgcacaac agcagacgtg aaagttgacg attgatcatt
    17221 gcgtggctcc ttgtgcctgt tccgtggcgg gcgttaatgc cggggctgtc cagtctggca
    17281 agcgggcgaa aagctggcag acgtgttgct cctgttttaa taaggtgggc aggatggcct
    17341 gcgaggcggg tgtctcttca agcgttattt gaggagaacg gctgagtgcg gttgccagac
    17401 cggcagcgta tttttccagg gcgtcggcaa actgacctgc tctgtccggc tgtagtgcgt
    17461 tatccagcgc ctgcgccgaa ttccaggtgg catcccaggc gtgaaggata tcgcggccct
    17521 gacgaataac cgtttgcgaa cgttcaatca gtaaggcgcg ttcttcgctg tccagttgac
    17581 gctccagcgc cacgcgttgg cacatctctt cacaggcatt aaacgccgca tgcagaccga
    17641 tacgaatttg cagataagtg cgcagagccg tgacttcctg ctggcgtggg atccgcatta
    17701 ctttacttaa catacccagc gcgccagcca gtttttgcgg cagcgtgcgc gcttcacttt
    17761 caggccagac aaaggtgtaa atcaccgcgg acaccacggt accaatgatg atacccaggg
    17821 cgcgatcgcg aatttccacc aggtcgtaca ctgggccaaa aacgttttcg agcgtggcga
    17881 gcgcgaaggt gaccaccatc tgtgtgccga tataagaaga gcgttcagag ctggtggcaa
    17941 tccatgcgcc caacaggaaa atcggtgcca gcacaaacag caattcgaca atattgtcca
    18001 gccagggcat gaccagtagc gtgaataaca gcgccagaat cgcgccgcaa aaggccccgc
    18061 caaaacgcag caccatcttc tggtacgacg aaccgacatt tggattagag acgatcacgc
    18121 aggtcagcat acaggtgtga atgccttccc agtccacgcc gctgtaaaag gtgtaacaga
    18181 tcaaacaggc gagcaatgtt tttaccgcgt agcgtatata gtctggattg gtaaaagcat
    18241 cggcgaccat tgatggcggt ttggcggcgg gcgttggcgg cgtattcggg ttcatctggc
    18301 ccagttgtaa caacgtctgg cagatattct ccaggttaca ttcccgtgcc gccaccgctt
    18361 cactttcact gatccgccag tcgctttgcc agcactgacc ttcagcaacg gcatgctgca
    18421 gcttgttgat ttctgaggct aatttttgcc gaaattcaat aattgcctga gaatcagcaa
    18481 aagaggtggg atcgtagcga ttcagcgtcg agtaaatgta ggttaccgtt gccacgcagc
    18541 tttgccacca tgcgctttga gttcgccagt tggcatcgtc cgcgagacaa aagacattca
    18601 gtttttgcag cgccagcgcc tctctttcaa tccgcgtttc gggtagcggt gcgaggctgt
    18661 ctgtcaggtg actaatggca tcatcaagcc gatcattaag cgcctgatgc atttgcgaaa
    18721 tggcacgact gggaaaccac agcacgccga ttaacgtcat cagcaaggtt ggatagaggc
    18781 caacaacgat acaccacagc gttaagcgca cgaccacttc cggatagtcg agcatggcgg
    18841 ggaaggtttg cccgtaaatg gcgacaatgg cgacagcgaa aaagaccagt cccagccgat
    18901 gggtgcgcat caaaaacatg cggcccatca ggatcggccc ggcgatgatc aatcggatca
    18961 acggttcgcc gtatgaccat ttgtagatca aaaacaggct gccgatctcc agcaccgtgg
    19021 caaccacaaa caagatcgcc acaaatttgg tgtaaaacgc gttcgactga ataccgtaaa
    19081 acagcactgc cagcgataac gccacaaaag ggatctcaaa agtcatcgag atcagaatca
    19141 ccagcaggca gcctacccag agttgcacga tctgcggcac gcggccgggt cgccgctcgc
    19201 ttaactcttc atgaaagaac gccagcagcc tgaccatcgg taatggcagg gagttgagcg
    19261 cgctcattat tgcggctcaa gattagcgac tgccgaagcg ccgatgcgga acatttccgg
    19321 gtcaggttta tcgaccatga ttttgaccgg aaaacgctgg gcaacgcgga cccagttaat
    19381 agagcgagaa actttcggca ggccgcccag caccaggccg ccgtcatccg gtagcacgcc
    19441 gtagccaatc gaatccactt taccctcgaa ggttttgccg ctgtcactca tcaggcgaat
    19501 cgttgcgggt gtacctgagc gaatattttt cagatcggtt tcgcggaagt tggcgatcac
    19561 gtaccagtga cgagtgtcga ttagggtaaa aatagggcgc atggcagaag caaattgccc
    19621 gacggaggtt ttgagggaaa tgacccggcc atcaaacggc gcgcgaacgg tcgccatttc
    19681 cagatgcagt ttggtcaggg caatatccgc ttcgaccgcc gcacgttgcg ccaccagcgc
    19741 atcgacgccg ctgacggcgc tggcggctga ctgcgcctgt aacaatacgg cattaagatc
    19801 ggcttctgcg gcgcgctgcg cagttcttgc acggtcaacg tcttccgctg agacaaaacc
    19861 ctctttcagt aatggctcgg tacggcgtaa tgtatctgtg gcctgtttcg cggcggcacg
    19921 ggctttttct accgtggcat taatcgagtc ggcaccaaac tgttgcgcat caacgctacg
    19981 ctgggtgagc ataatttgct tatccagcgc cgcgagggag gcttcagctt tcgccagatt
    20041 ggcttcgtac gggcgcgggt caatgcggaa cagcaaatcg ccctgtttga ctgcctggtt
    20101 gtcggtgacc gccagttcta caatgcggcc gctgacttcc ggcaccacat caatggtatc
    20161 tgctgacgcg taagcgtcat tagttgatgg cgcactgtct acgcgccaga taacgaaaac
    20221 aagggcaacc agcgccaatg caaccactaa cagagcaggg aatttactgc gagg
//
LOCUS       DAZOYL010000044        20235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZOYL010000044 DAZOYL010000000
VERSION     DAZOYL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.044"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(172..984)
                     /locus_tag="ACHUOY_001180"
     CDS             complement(172..984)
                     /locus_tag="ACHUOY_001180"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR0682011.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            1246..2007
                     /gene="udp"
                     /locus_tag="ACHUOY_001181"
     CDS             1246..2007
                     /gene="udp"
                     /locus_tag="ACHUOY_001181"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR0682012.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2148..3575
                     /gene="rmuC"
                     /locus_tag="ACHUOY_001182"
     CDS             2148..3575
                     /gene="rmuC"
                     /locus_tag="ACHUOY_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR0682013.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3670..4425
                     /gene="ubiE"
                     /locus_tag="ACHUOY_001183"
     CDS             3670..4425
                     /gene="ubiE"
                     /locus_tag="ACHUOY_001183"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR0682014.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4439..5044
                     /gene="ubiJ"
                     /locus_tag="ACHUOY_001184"
     CDS             4439..5044
                     /gene="ubiJ"
                     /locus_tag="ACHUOY_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR0682015.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5041..6681
                     /gene="ubiB"
                     /locus_tag="ACHUOY_001185"
     CDS             5041..6681
                     /gene="ubiB"
                     /locus_tag="ACHUOY_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR0682016.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6760..7029
                     /gene="tatA"
                     /locus_tag="ACHUOY_001186"
     CDS             6760..7029
                     /gene="tatA"
                     /locus_tag="ACHUOY_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR0682017.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7033..7548
                     /gene="tatB"
                     /locus_tag="ACHUOY_001187"
     CDS             7033..7548
                     /gene="tatB"
                     /locus_tag="ACHUOY_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR0682018.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7551..8327
                     /gene="tatC"
                     /locus_tag="ACHUOY_001188"
     CDS             7551..8327
                     /gene="tatC"
                     /locus_tag="ACHUOY_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR0682019.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8369..9151
                     /gene="tatD"
                     /locus_tag="ACHUOY_001189"
     CDS             8369..9151
                     /gene="tatD"
                     /locus_tag="ACHUOY_001189"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR0682020.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(9148..9636)
                     /gene="rfaH"
                     /locus_tag="ACHUOY_001190"
     CDS             complement(9148..9636)
                     /gene="rfaH"
                     /locus_tag="ACHUOY_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR0682021.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9803..11296
                     /gene="ubiD"
                     /locus_tag="ACHUOY_001191"
     CDS             9803..11296
                     /gene="ubiD"
                     /locus_tag="ACHUOY_001191"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR0682022.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11343..12044
                     /gene="fre"
                     /locus_tag="ACHUOY_001192"
     CDS             11343..12044
                     /gene="fre"
                     /locus_tag="ACHUOY_001192"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR0682023.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12234..13397)
                     /gene="fadA"
                     /locus_tag="ACHUOY_001193"
     CDS             complement(12234..13397)
                     /gene="fadA"
                     /locus_tag="ACHUOY_001193"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR0682024.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13407..15596)
                     /gene="fadB"
                     /locus_tag="ACHUOY_001194"
     CDS             complement(13407..15596)
                     /gene="fadB"
                     /locus_tag="ACHUOY_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR0682025.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15786..17117
                     /gene="pepQ"
                     /locus_tag="ACHUOY_001195"
     CDS             15786..17117
                     /gene="pepQ"
                     /locus_tag="ACHUOY_001195"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR0682026.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17117..17731
                     /locus_tag="ACHUOY_001196"
     CDS             17117..17731
                     /locus_tag="ACHUOY_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR0682027.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17770..19221
                     /gene="trkH"
                     /locus_tag="ACHUOY_001197"
     CDS             17770..19221
                     /gene="trkH"
                     /locus_tag="ACHUOY_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR0682028.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19233..19778
                     /gene="hemG"
                     /locus_tag="ACHUOY_001198"
     CDS             19233..19778
                     /gene="hemG"
                     /locus_tag="ACHUOY_001198"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR0682029.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            20156..>20235
                     /locus_tag="ACHUOY_001199"
     rRNA            20156..>20235
                     /locus_tag="ACHUOY_001199"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         4736 a         5131 c         5503 g         4865 t
ORIGIN      
        1 ggtgtcttcc attgaagaga ctcatttatg actatgactt gccgactttc ttaaggtcaa
       61 atagcggagt cgtaatctcc aatcaattca tactgattat tttttggggg acagagatgg
      121 cagcaaaacg cactcgttta ctcaccatct caaatataga ctaagaggaa actaactccg
      181 cttctctcca tactgcttaa accattccag catacgctgc cagccatctt tcgcagattc
      241 ggcatgatag ctcgggcgat aatcagcgtt gaatgcatgc ccggcatccg ggtacacgat
      301 aatctctgct ttcgcattag cagcccgcag cgcctggcgc atggtttcaa cgctctcctg
      361 cggaatgctg ttatcctgac cgccatataa gccgagaatc ggcgcgttaa gatcggttgc
      421 gatatcaaca ggttgtttcg gtgaattcag cgacttgtcg cccgtcagtt tgccgtacca
      481 cgccactgca gcttttagct gtggattatg cgcggcatac agccaggtga tacgtccacc
      541 ccagcagaat ccggtgatca ttaaacgatg aacatcgccg ccgttgcggg aagcccaact
      601 ggcggcatga tcgagatcgg ccagcacctg cgagtcaggc acttttgcta ccagaccgct
      661 aagcaacgtg gggatatcgg caaaatcatt cggatcgcct tcgcggaagt aaagttcagg
      721 tgcgatagcc agatacccct ccagcgccag acggcgacaa atgtcgcgga tatgttcatg
      781 cacgccaaaa atttcctgca ctacaatgac cactggcagt gggccatcgc tttgctttgg
      841 tctggcatgg taagcaggca tgttatcccc ttgtgaaggg atagaggtga agcccgccac
      901 aatcgcgtcg tccagggtct gaacgatggt cgaagcgaga ggcgatgcag caggtgcaaa
      961 tccagattgt tgtgttgttg ccatggtatt ctccgtaccc ttataaaaat gttgcgcaat
     1021 gttaactata gtcagcatgc aacaaatcac attgcctgaa tcggctcatc ttttatgcag
     1081 tcctgcagaa tgaagagtga tttatgtgat ttgaatcact tttggtgggt aaatttatgc
     1141 aacgcatttg cgtcatggtg atgagtatca cgaaaaaatg ttaaaccctt cggtaaagtg
     1201 tctttttgct tcttctgact aaaccgattc acagaggagt tgtatatgtc caagtctgat
     1261 gtttttcatc tcggcctcac taaaaacgat ttacaagggg ctacgcttgc catcgtccct
     1321 ggcgacccgg atcgtgtgga aaagatcgcc gcgctgatgg ataagccggt taagctggca
     1381 tctcaccgcg aattcactac ctggcgtgca gagctggatg gtaaacctgt tatcgtctgc
     1441 tctaccggta tcggcggccc gtctacctct attgctgttg aagagctggc acagctgggc
     1501 attcgcacct tcctgcgtat cggtacaacg ggcgctattc agccgcatat taatgtgggt
     1561 gatgtcctgg ttaccacggc gtctgtccgt ctggatggcg cgagcctgca cttcgcaccg
     1621 ctggaattcc cggctgtcgc tgatttcgaa tgtacgactg cgctggttga agctgcgaaa
     1681 tccattggcg cgacaactca cgttggcgtg acagcttctt ctgatacctt ctacccaggt
     1741 caggaacgtt acgatactta ctctggtcgc gtagttcgtc actttaaagg ttctatggaa
     1801 gagtggcagg cgatgggcat aatgaactat gaaatggaat ctgcaaccct gctgaccatg
     1861 tgtgcaagtc agggcctgcg tgccggtatg gtagcgggtg ttatcgttaa ccgcacccag
     1921 caagagatcc cgaatgctga gacgatgaaa caaactgaaa gccaagcggt gaaaatcgtg
     1981 gtggaagcgg cgcgtcgtct gctgtaattc tcttctcctg tctgaaggcc gacgcgttcg
     2041 gccttttgta tttttgcgta gcgcctcgca ggaaatgcct ttccaactgg acgtttgtac
     2101 agcacaattc tattttgtgc gggtaagttg ttgcgtcagg aggcgttgtg gatttctcaa
     2161 tcatggttta cgcagttatt gcgttggtgg gtgtggcaat tggctggctg tttgccagtt
     2221 atcaacatgc gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg gtcgcggagt
     2281 taagcgcggc aaaacaacaa attacccaaa gcgagcactg gcgtgcagag tgcgagttac
     2341 tcaataacga agtgcgcagc ctgcaaagta ttaacacctc tctggaggcc gatctgcgtg
     2401 aagtaaccac gcggatggaa gccgcacagc aacatgctga cgataaaatt cgccagatga
     2461 ttaacagcga gcagcgcctc agtgagcagt ttgaaaacct cgccaaccgt atttttgagc
     2521 acagcaatcg ccgggttgat gagcaaaacc gtcagagtct gaacagcctg ttgtcgccgc
     2581 tacgtgaaca actggacggt ttccgccgtc aggttcagga cagcttcggt aaagaagcac
     2641 aagaacgcca taccctgacc cacgaaattc gcaatctcca gcaactcaac gcgcaaatgg
     2701 cccaggaagc gatcaacctg acgcgcgcgc tgaaaggcga caataaaacc cagggcaact
     2761 ggggcgaggt agtattgacg cgggtgctgg aggcttccgg tctgcgtgaa gggtatgaat
     2821 atgaaaccca ggtcagcatc gaaaatgacg cccgctcgcg gatgcagccg gatgtcatcg
     2881 tgcggctgcc gcagggaaaa gatgtggtga tcgacgccaa aatgacgctg gtcgcctatg
     2941 aacgctattt taacgccgaa gacgactaca cccgcgaaag cgcgctacag gaacatatcg
     3001 cgtcggtgcg taaccatatc cgtttgctgg gacgcaaaga ttatcaacag ctgccggggc
     3061 tgcgaactct ggattacgtg ctgatgttta ttcccgttga acccgctttt ttactggcgc
     3121 ttgaccgcca gccggagctg atcaccgaag cgttgaaaaa caatatcatg ctggttagcc
     3181 cgactacgct gctggtggcg ttgcgtacta ttgccaacct gtggcgttat gagcatcaaa
     3241 gccgcaacgc ccagcaaatc gccgatcgtg ccagcaagct gtacgacaag atgcgtttgt
     3301 tcatcgatga catgtccgcg attggtcaaa gtctcgacaa agcgcaggat aactaccgcc
     3361 aggcaatgaa aaaactctct tcaggtcgcg gaaatgtgct ggcgcaggca gaagcgtttc
     3421 gcggtttagg agtagaaatt aaacgcgaga ttaatccgga tttggctgaa caggcggtga
     3481 gccagaatga agagtatcga cttcggtcgg ttccggagca gccgaatgat gaagcttatc
     3541 aacgcgatga tgaatataat cagcagtcgc gctagcccat taggggtagt taagccgggt
     3601 agaaatctag ggcatcgacg cccaatctgt tacacttctg gaacaatttt ttgatgagca
     3661 ggcattgaga tggtggataa gtcacaagaa acgacgcact ttggttttca gaccgtcgcg
     3721 aaggaacaaa aagcggatat ggtcgcccac gttttccatt ccgtggcatc aaaatacgat
     3781 gtcatgaatg atttgatgtc atttggtatt catcgtttgt ggaagcgatt cacgattgat
     3841 tgcagcggcg tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac cggcgacctg
     3901 acagcgaaat tctcccgcct ggtcggagaa actggcaaag tggtccttgc tgatatcaat
     3961 gaatccatgc tcaaaatggg acgcgagaag ctgcgtaata tcggtgtgat tggcaacgtt
     4021 gagtatgttc aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt tgattgcatc
     4081 accatttcgt ttggtctgcg taacgtcacc gacaaagata aagcactgcg ttcaatgtat
     4141 cgcgtgctga aacccggcgg ccgcctgctg gtgcttgagt tctcgaagcc aattatcgag
     4201 ccgctgagca aagcctatga tgcatactcc ttccatgtgc tgccgcgtat tggctcactg
     4261 gtcgcgaacg acgccgacag ctaccgttat ctggcagaat ccatccgtat gcatcccgat
     4321 caggataccc tgaaagccat gatgcaggat gccggattcg aaagtgtcga ctactacaat
     4381 ctgacggcag gggttgtggc gctgcatcgt ggttataagt tctgacagga gaccggaaat
     4441 gccttttaaa cctttagtga cggcaggaat tgaaagtctg ctcaacacct tcctgtatcg
     4501 ctcacccgcg ctgaaaacag cccgctcgcg tctgctgggt aaagtattgc gcgtggaggt
     4561 aaaaggcttt tcgacgtcat tgattctggt gttcagcgaa cgccaggttg atgtactggg
     4621 cgaatgggca ggcgatgctg actgcaccgt tatcgcctac gccagtgtgt tgccgaaact
     4681 tcgcgatcgc cagcagctta ccgcactgat tcgcagtggt gagctggaag tgcagggcga
     4741 tattcaggtg gtgcaaaact tcgttgcgct ggcagatctg gcagagttcg accctgcgga
     4801 actgctggcc ccttataccg gtgatatcgc cgctgaagga atcagcaaag ccatgcgcgg
     4861 aggcgcaaag ttcctgcatc acggcattaa gcgccagcaa cgttatgtgg cggaagccat
     4921 tactgaagag tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg cggaagagac
     4981 ggctgccgtc gagcgtgctg ttgatgccct gaccaaacgg ctggaaaaac tggaggctaa
     5041 atgacgccag gtgaagtacg gcgcctatat ttcatcattc gcactttttt aagctacgga
     5101 cttgatgaac tgatccccaa aatgcgtatc accctgccgc tacggctatg gcgatactca
     5161 ttattctgga tgccaaatcg gcataaagac aaacttttag gtgagcgact acgactggcc
     5221 ctgcaagaac tggggccggt ttggatcaag ttcgggcaaa tgttatcaac ccgccgcgat
     5281 ctttttccac cgcatattgc cgatcagctg gcgttattgc aggacaaagt tgctccgttt
     5341 gatggcaagc tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc ggtagaagcg
     5401 tggtttgacg attttgaaat caagccactg gcttctgctt ctatcgccca ggttcatacc
     5461 gcgcgattga aatcgaatgg taaagaggtg gtgattaaag tcatccgccc ggatattttg
     5521 ccggttatta aagcggatct gaaacttatc taccgtctgg ctcgctgggt gccgcgtttg
     5581 ctgccggatg gtcgccgtct gcgcccaacc gaagtggtgc gcgagtacga aaagaccttg
     5641 attgatgaac tgaatttgct gcgggaatct gccaacgcca ttcagcttcg gcgaaatttt
     5701 gaagacagcc cgatgctcta catcccggaa gtttaccctg actattgtag tgaagggatg
     5761 atggtgatgg agcgcattta cggcattccg gtgtctgatg ttgcgacgct ggagaaaaat
     5821 ggcaccaaca tgaaattgct ggcggaacgt ggcgtgcagg tgttcttcac tcaggtcttt
     5881 cgcgacagct ttttccatgc cgatatgcac cctggcaaca ttttcgtaag ctatgaacac
     5941 ccggaaaacc cgaaatatat cggcattgat tgcgggattg ttggctcgct aaacaaagaa
     6001 gataaacgct atctggcaga aaactttatc gccttcttta atcgcgacta tcgcaaagtg
     6061 gcagagctac acgtcgattc tggctgggtg ccaccagata ccaacgttga agagttcgaa
     6121 tttgccattc gtacggtctg tgaacctatc tttgagaaac cgctggccga aatttcgttt
     6181 ggacatgtac tgttaaatct gtttaatacg gcgcgtcgct tcaatatgga agtgcagccg
     6241 caactggtgt tactccagaa aaccctgctc tatgtcgaag gggtaggacg ccagctttat
     6301 ccgcagctcg atttatggaa aacagcgaag cctttcctgg agtcgtggat taaagatcag
     6361 gtcggtattc ctgccctggt gagagcattt aaagaaaaag cgccgttctg ggtcgaaaaa
     6421 atgccagaac tgcctgaatt ggtttacgac agtttgcgcc agggcaagta tttacagcac
     6481 agtgttgata agattgcccg cgagcttcag tcaaatcatg tacgtcaggg acaatcgcgt
     6541 tattttctcg gaattggcgc tacgttagta ttaagtggca cattcttgtt ggtcagccga
     6601 cctgaatggg ggctgatgcc cggctggtta atggcaggtg gtctgatcgc ctggtttgtc
     6661 ggttggcgca aaacacgctg attttttcat cgctcaaggc gggtcgtgta acgtataatg
     6721 cggccttgtt taatcatcat ctaccacaga ggaacatgta tgggtggtat cagtatttgg
     6781 cagttattga ttattgccgt catcgttgta ctgctttttg gcaccaaaaa gctcggctcc
     6841 atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag cgatgatgaa
     6901 ccaaagcagg ataaaaccag tcaggatgct gattttactg cgaaaactat cgccgataag
     6961 caggcggata cgaatcagga acaggctaaa acagaagacg cgaagcgcca cgataaagag
     7021 caggtgtaat ccgtgtttga tatcggtttt agcgaactgc tattggtgtt catcatcggc
     7081 ctcgtcgttc tggggccgca acgactgcct gtggcggtaa aaacggtagc gggctggatt
     7141 cgcgcgttgc gttcactggc gacaacggtg cagaacgaac tgacccagga gttaaaactc
     7201 caggagtttc aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa cctgacgccc
     7261 gaactgaaag cgtcgatgga tgaactacgc caggccgcgg agtcgatgaa gcgttcctac
     7321 gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca ccatccataa cccggtggtg
     7381 aaagataatg aagctgcgca tgagggcgta acgcctgccg ctgcacaaac gcaggccagt
     7441 tcgccggaac agaagccaga aaccacgcca gagccggtgg taaaacctgc tgcggacgct
     7501 gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac atgtctgtag
     7561 aagatactca accgcttatc acgcatctaa ttgagctgcg taagcgtctg ctgaactgca
     7621 ttatcgcggt gatcgtgata ttcctgtgtc tggtctattt cgccaatgac atctatcacc
     7681 tggtatccgc gccattgatc aagcagttgc cgcaaggttc aacgatgatc gccaccgacg
     7741 tggcctcgcc gttctttacg ccgatcaagc tgacctttat ggtgtcgctg attctgtcag
     7801 cgccggtgat tctctatcag gtgtgggcat ttatcgcccc agcgctgtat aagcatgaac
     7861 gtcgcctggt ggtgccgctg ctggtttcca gctctctgct gttttatatc ggcatggcat
     7921 tcgcctactt tgtggtcttt ccgctggcat ttggcttcct tgccaatacc gcgccggaag
     7981 gggtgcaggt atccaccgac atcgccagct atttaagctt cgttatggcg ctgtttatgg
     8041 cgtttggtgt ctcctttgaa gtgccggtgg caattgtgct gctgtgctgg atggggatta
     8101 cctcgccaga agacttacgc aaaaaacgcc cgtatgtgct ggttggtgca ttcgttgtcg
     8161 ggatgttgct gacgccgccg gatgtcttct cgcaaacgct attggcgatc ccgatgtact
     8221 gtctgtttga aatcggtgtc ttcttctcac gcttttacgt tggtaaaggg cgaaatcggg
     8281 aagaggaaaa cgacgctgaa gcagaaagcg aaaaaactga agaataaatt caaccgcccg
     8341 tcagggcggt tgtcatatgg agtacaggat gtttgatatc ggcgttaatt tgaccagttc
     8401 gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct tttgacgcgg gagttaatgg
     8461 gctactcatc accggcacta acctgcgtga aagccagcag gcgcaaaagc tggcgcgtca
     8521 gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac gacagcagcc agtggcaagc
     8581 tgcgactgaa gaagcgatta ttgagctggc cgcgcagcca gaagtggtgg cgattggtga
     8641 atgtggtctc gactttaacc gcaacttttc gacgccggaa gagcaggaac gcgcttttgt
     8701 tgcccagcta cgcattgccg cagatttaaa catgccggta tttatgcact gtcgcgatgc
     8761 ccacgagcgg tttatgacat tgctggagcc gtggctggat aaactgcctg gtgcggttct
     8821 tcattgcttt accggcacac gcgaagagat gcaggcgtgc gtggcgcatg gaatttatat
     8881 cggcattacc ggttgggttt acgatgaacg acgcggactg gagctgcggg aacttttgcc
     8941 gttgattccg gcggaaaaat tactgatcga aactgatgcg ccgtatctgc tccctcgcga
     9001 tctcacgcca aagccatcat cccggcgcaa cgagccagcc catctgcccc atattttgca
     9061 acgtattgcg cactggcgtg gagaagatgc cgcatggctg gctgccacca cggatgctaa
     9121 tgtcaaaaca cggtttggga ttgcgtttta gagtttgcgg aactcggtgt tcttcacact
     9181 gtgcttaatc tctttattaa taagattaag caatagcatg gagcgagcct caccatcggg
     9241 ttcggtgaaa atggcctgaa agccttcgaa cgcgccttcg gtaataatca ccttatctcc
     9301 cggataaggg gttgccggat cgacaatgtc tttcggttta tataccgata gctgatgaat
     9361 caccgccgat gggactatcg ctagcgacgc gccaaagcgc acgaagtggc tgacaccgcg
     9421 ggtcgcgttg atagtcgtgg tatgaatcac ttctggatca aattcgacaa acaggtagtt
     9481 gggaaacaat ggctcactga ctgcagtacg ttttccacgc acgatttttt ccagggtgat
     9541 catcggtgcc aggcaattca cagcctgtct ctcaaggtgt tcctgggcac gttgaagttg
     9601 cccgcgcttg cagtacagta aataccaaga ttgcataatg actcttatcc gtttaatcgg
     9661 ggcgcaagga tagcaaaagc tttacgctaa gttaattata ttccccggtt tgcgttatac
     9721 cgtcagagtt cacgctaatt taacaaattt acagcatcgc aaagatgaac gccgtataat
     9781 gggcgtagat taagaggcta caatggacgc catgaaatat aacgatttac gcgacttctt
     9841 gacgctgctt gaacagcagg gtgagctaaa acgtatcacg ctcccggtgg atccgcatct
     9901 ggaaatcact gaaattgctg accgcacttt gcgtgccggt gggcctgcgc tgttgttcga
     9961 aaaccctaaa ggctactcaa tgccggtgct gtgcaacctg ttcggtacgc caaagcgcgt
    10021 ggcgatgggc atggggcagg aagatgtttc ggcgctgcgt gaagttggta aattattggc
    10081 gtttctgaaa gagcccgagc cgccaaaagg tttccgcgac ctgtttgata aactgccgca
    10141 gtttaagcaa gtattgaaca tgccgacaaa gcggctgcgt ggtgcgccct gccaacaaaa
    10201 aatcgtctct ggcgatgacg tcgatctcaa tcgcattccc attatgacct gctggccgga
    10261 agatgccgcg ccgctgatta cctgggggct gaccgtaacg cgcggcccgc ataaagagcg
    10321 gcagaatctg ggcatttatc gccagcagct gattggtaaa aacaaactga ttatgcgctg
    10381 gctgtcgcat cgcggcggcg cgctggatta tcaggagtgg tgtgcggcgc atccgggcga
    10441 acgtttcccg gtttctgtgg cgctgggtgc cgatcccgcc acgattctcg gtgcagtcac
    10501 ccccgttccg gatacacttt cagagtatgc gtttgccgga ttgctgcgcg gtaccaagac
    10561 cgaagtggtg aagtgtatct ccaatgacct tgaagtgccc gccagtgcgg agattgtgct
    10621 ggaagggtat atcgaacaag gcgaaactgc gccggaaggg ccgtatggcg accacaccgg
    10681 ttactataac gaagtcgata gtttcccggt atttaccgtg acgcatatta cccagcgtga
    10741 agatgcgatt taccactcca cctatactgg tcgtccgccg gatgaacccg cggtgctggg
    10801 tgtcgcactg aacgaagtgt ttgtgccgat tctgcaaaaa cagttcccgg aaattgtcga
    10861 tttttacctg ccaccggaag gctgttctta tcgtctggcg gtagtgacaa tcaaaaaaca
    10921 gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac gccagtttat
    10981 gtacactaaa tttgtgatcg tttgcgatga tgacgtcaac gcacgcgact ggaacgatgt
    11041 gatttgggcg attaccaccc gtatggaccc ggcgcgggat actgttctgg tagaaaatac
    11101 gcctattgat tatctggatt ttgcctcgcc tgtctccggg ctgggttcaa aaatggggct
    11161 ggatgccacg aataaatggc cgggggaaac ccagcgtgaa tggggacgtc ccatcaaaaa
    11221 agatcctgat gtcgtcgcgc atattgacgc catctgggat gaactggcta tttttaacaa
    11281 cggtaaaagc gcctgatgcg cgttagtttt gccctattta tttgatccga cagagaaagc
    11341 gcatgacaac cttaagctgt aaagtgacct cggtagaagc tatcacggat actgtatacc
    11401 gtgtccgaat cgtaccagac gcggcctttt cttttcgtgc tggtcagtat ttgatggtag
    11461 tgatggatga gcgcgacaaa cgtccgtttt caatggcttc gacgccggat gaaaaagggt
    11521 ttatcgagct gcatattggc gcttctgaaa tcaaccttta cgcgaaagca gtcatggacc
    11581 gcatcctcaa agatcatcaa atcgtggtcg atattcccca cggagaagcg tggctgcgcg
    11641 atgatgaaga gcgtccgatg attttgattg cgggcggcac cgggttctcc tatgcgcgct
    11701 cgattttatt gacggcgttg gcgcgtaatc ccaatcgcga tatcaccatt tactggggtg
    11761 ggcgtgaaga gcaacatctg tatgatctct gcgagcttga ggcgctttcg ctgaagcatc
    11821 ctggtctgca agtggtgccg gtggttgaac aaccggaagc gggctggcgt gggcgtactg
    11881 gcaccgtgtt aacggcggtc ttgcaggatc atggtacgct ggcagagcat gatatctata
    11941 ttgccggacg ttttgagatg gcgaaaattg ctcgtgacct gttttgcagt gagcgtaatg
    12001 cgcgggaaga tcgtctgttt ggcgatgcgt ttgcatttat ctgagatata aaaacccgcc
    12061 cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag atctatgtcg
    12121 catccggcaa tcggtgcacg atgcctgatg cgacgctggc gcgtcttatc aggcctacat
    12181 tggtgcagga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
    12241 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
    12301 tgaacgtctt tgtgttccat cagattcagc aacgtggtgc tgatacgcgc accggaacaa
    12361 cccagcggat gacccagcgc gatcgcacca ccattgaggt tgatcttctc gtcaatctgc
    12421 tccattagcc cgagatcttt aatgcacggc aggatctgcg cggcaaaggc ttcgttcatc
    12481 tcaaacacgc cgatatcgct ggcagaaagc cccgcttttt tcagcgccag cttcgaggca
    12541 ggaaccgggc cgtaacccat aatcgacgga tcacaaccaa cgaccgccat cgaacgcaca
    12601 cgggcgcgcg gcttaagacc taattcacgg gcgcggcttt cactcatcac cagcatggcc
    12661 gcggcaccat cagaaagtgc agaagatgta cccgccgtta ccgtaccgct tactggatca
    12721 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
    12781 taattaaact gcttcaggac gccgtcggca tcgtgaccac cggtcgggat gatttcattt
    12841 ttaaatgcgc ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
    12901 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
    12961 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtgactc
    13021 atcggcacat ggcccatatg ctccacgccg ccaaccagac ctgcctgcgc atcgccagtc
    13081 atgatcattc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa gcgattgacg
    13141 gtaaccgccg ggacagagtg cggcacttct gccagcagcg ccgcgttacg ggcgatatta
    13201 aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
    13261 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttctgca
    13321 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
    13381 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
    13441 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
    13501 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
    13561 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
    13621 accagcgcca tatccgcttc cgccggagtg gcgataatgc cctcttccag acagcgcacc
    13681 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
    13741 ttcggctggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
    13801 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
    13861 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tttgcatccg ctgcgggaag
    13921 cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa tgcccacaac gtccagcaga
    13981 tatgccgggc ccatcggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
    14041 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
    14101 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
    14161 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
    14221 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttccgg gcgttccagc
    14281 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatcctgg
    14341 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcaacaacc
    14401 gcttctacca caatatccac gcggtcaaat ccggcgtagt cgagcgttgg gtggatcgtg
    14461 gagatcacgc cagccagttt cagaccatcg atcttgccgc gctcaagctg cttgttcagc
    14521 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
    14581 accggcacgc ctttccacgc ggactggtaa gcgataccgc cacccatgat gcctgcgccc
    14641 agcacagcag cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
    14701 acatattgat cgttaaggaa aatgccgacc agtgcgcgag cttcgttggt atgcgccagc
    14761 gggacaaaac ttttgttttc cagatttaag gcttcttcac gaccaaaacg ggccgcagct
    14821 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgccgt ttgcgcgacc
    14881 attcctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagcggttcc
    14941 agcttcggct ggcgtttcgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaacc
    15001 gcctttgcgc cttcaaccag tttttctgct ttgactaccc catccaccag accgattttc
    15061 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc actgtcagcg
    15121 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
    15181 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
    15241 tcgcagccac cacccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
    15301 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
    15361 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
    15421 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
    15481 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
    15541 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
    15601 gtctcctgaa tccgcaaggt gatctggtcg taccagatgc atcgaagtgt gtattttgtg
    15661 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
    15721 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
    15781 aaaaaatgga atcactggcc tcgctctata aaaatcatat agctacctta caagaacgga
    15841 ctcgcgatgc gctggcgcgc ttcaagctgg atgcattact tattcactcc ggcgagctgt
    15901 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
    15961 gggtgccggt aactcaggtg ccaaactgct ggttgctggt ggatggcgtg aacaagccga
    16021 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctcct
    16081 tctggactga agatgtggaa gtgatcgcac tgccgaaagc cgatggcatt ggtagcctgc
    16141 tgcccgctgc gcgcggcaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
    16201 tgggtattga ggccagcaac atcaacccga aaggggtgat cgactacctg cattactacc
    16261 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
    16321 acggtcatcg tgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaaca
    16381 tcgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
    16441 cgcttaacga acacgcttcg gtgctgcatt acaccaaact ggatcatcag gcaccggaag
    16501 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
    16561 ctcgtacctg gtcggcaaaa agcgacaacg actacgcaca gctggtgaaa gacgtaaatg
    16621 atgaacaact ggcgctgatc gccaccatga aagcaggcgt tagctatgtg gattaccaca
    16681 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
    16741 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcat ggtatcggtc
    16801 atccgctggg cctgcaggtg catgacgtag ccggttttat gcaggatgat agcggtacac
    16861 acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc cagccgggta
    16921 tggtgttaac catcgaaccg ggtatctact tcattgaatc gctactggca ccgtggcgtg
    16981 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
    17041 gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg
    17101 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
    17161 agagatcaaa aaaagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
    17221 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
    17281 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
    17341 ggcaggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat
    17401 tacctctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
    17461 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
    17521 attaaccgaa tatactttgc aatgtgaata tcatcagtta accggcattg aagcgttgct
    17581 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
    17641 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
    17701 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
    17761 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
    17821 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
    17881 gcttttaccc agaccttttt tgtcgccctc gccattggtt ctatgctgtg gtggccgaac
    17941 cgtaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt gctgttctgg
    18001 accgtgctgg gtagtgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
    18061 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
    18121 gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct gcaatggttt
    18181 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
    18241 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
    18301 attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
    18361 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
    18421 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagcccgact
    18481 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
    18541 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
    18601 atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt tcataatgtc
    18661 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc gatggcgaca
    18721 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
    18781 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
    18841 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
    18901 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
    18961 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
    19021 acgggcgtgg atgacttttc tgcctttgct tcagttgttg cgacattgaa taacctgggg
    19081 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
    19141 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt gctctttacc
    19201 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
    19261 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
    19321 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aattatgacc
    19381 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
    19441 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
    19501 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
    19561 tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg cgttacccac
    19621 gttatcgctg gtacgatcgt tttatgatca agctaattat gaagatgtca ggcggtgaaa
    19681 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
    19741 aaatcgccca gttaaccaac aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc
    19801 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    19861 accactgaca cggaacaacg gcaaacacgc cgccgggtca gcggggttct cctgagaatt
    19921 ccgacagaga aagcaaaaat aaatgcttga ctctgtagcg ggaaagcgta ttatgcacac
    19981 cccgcgccgc tgagaaaaag cgaagcggca ctgctcttta acaatttatc agacaatctg
    20041 tgtgggcact cgaagatacg gattcttaac gtcgcaagac gaaaaatgaa taccaagtct
    20101 caagagtgga cacgtaattc attacgaagt ttaattcttt gagcatcaaa cttttaaatt
    20161 gaagagtttg atcatggctc agattgaacg ctggcggcag gcctaacaca tgcaagtcga
    20221 acggtaacag gaagc
//
LOCUS       DAZOYL010000045        20145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZOYL010000045 DAZOYL010000000
VERSION     DAZOYL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.045"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            3..674
                     /gene="phoP"
                     /locus_tag="ACHUOY_001200"
     CDS             3..674
                     /gene="phoP"
                     /locus_tag="ACHUOY_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR0682030.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            674..2134
                     /gene="phoQ"
                     /locus_tag="ACHUOY_001201"
     CDS             674..2134
                     /gene="phoQ"
                     /locus_tag="ACHUOY_001201"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR0682031.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            2210..3331
                     /gene="roxA"
                     /locus_tag="ACHUOY_001202"
                     /gene_synonym="ycfD"
     CDS             2210..3331
                     /gene="roxA"
                     /locus_tag="ACHUOY_001202"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR0682032.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(3380..4606)
                     /gene="pepT"
                     /locus_tag="ACHUOY_001203"
     CDS             complement(3380..4606)
                     /gene="pepT"
                     /locus_tag="ACHUOY_001203"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR0682033.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            4856..5992
                     /gene="potA"
                     /locus_tag="ACHUOY_001204"
     CDS             4856..5992
                     /gene="potA"
                     /locus_tag="ACHUOY_001204"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR0682034.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            5976..6833
                     /gene="potB"
                     /locus_tag="ACHUOY_001205"
     CDS             5976..6833
                     /gene="potB"
                     /locus_tag="ACHUOY_001205"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR0682035.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            6830..7624
                     /gene="potC"
                     /locus_tag="ACHUOY_001206"
     CDS             6830..7624
                     /gene="potC"
                     /locus_tag="ACHUOY_001206"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR0682036.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            7621..8667
                     /gene="potD"
                     /locus_tag="ACHUOY_001207"
     CDS             7621..8667
                     /gene="potD"
                     /locus_tag="ACHUOY_001207"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR0682037.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(8823..9644)
                     /gene="cobB"
                     /locus_tag="ACHUOY_001208"
     CDS             complement(8823..9644)
                     /gene="cobB"
                     /locus_tag="ACHUOY_001208"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR0682038.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(9660..10571)
                     /gene="nagK"
                     /locus_tag="ACHUOY_001209"
     CDS             complement(9660..10571)
                     /gene="nagK"
                     /locus_tag="ACHUOY_001209"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR0682039.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(10600..11844)
                     /gene="lolE"
                     /locus_tag="ACHUOY_001210"
     CDS             complement(10600..11844)
                     /gene="lolE"
                     /locus_tag="ACHUOY_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR0682040.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(11844..12545)
                     /gene="lolD"
                     /locus_tag="ACHUOY_001211"
     CDS             complement(11844..12545)
                     /gene="lolD"
                     /locus_tag="ACHUOY_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR0682041.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(12538..13737)
                     /gene="lolC"
                     /locus_tag="ACHUOY_001212"
     CDS             complement(12538..13737)
                     /gene="lolC"
                     /locus_tag="ACHUOY_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR0682042.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            13999..15072
                     /locus_tag="ACHUOY_001213"
     CDS             13999..15072
                     /locus_tag="ACHUOY_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR0682043.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            15200..18646
                     /gene="mfd"
                     /locus_tag="ACHUOY_001214"
     CDS             15200..18646
                     /gene="mfd"
                     /locus_tag="ACHUOY_001214"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR0682044.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            18790..19746
                     /gene="ldtC"
                     /locus_tag="ACHUOY_001215"
                     /pseudo
     CDS             18790..19746
                     /gene="ldtC"
                     /locus_tag="ACHUOY_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(19829..20086)
                     /gene="bhsA"
                     /locus_tag="ACHUOY_001216"
                     /gene_synonym="ycfR"
     CDS             complement(19829..20086)
                     /gene="bhsA"
                     /locus_tag="ACHUOY_001216"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR0682045.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4879 a         5237 c         5148 g         4881 t
ORIGIN      
        1 aaatgcgcgt actggttgtt gaagacaatg cgttgttacg tcaccacctt aaagttcaga
       61 ttcaggatgc tggtcatcag gtcgatgatg cagaagatgc caaagaagcc gattattatc
      121 tcaatgaaca tttaccggat attgcgattg tcgatctcgg attgccagac gaggacggtc
      181 tgtcactgat tcgccgctgg cgtagcaatg atgtttcact gccgattctg gtattaaccg
      241 cccgtgaaag ctggcaggac aaagtcgaag tattaagtgc tggtgctgat gattacgtga
      301 ctaaaccatt tcatattgaa gaggtgatgg cgcgaatgca ggcattaatg cggcgtaata
      361 gcggtctggc ttcacaggtc atttcgctac caccgtttca ggttgatctc tctcgccgtg
      421 aattatctat taatgacgaa gtgatcaaac tgaccgcgtt cgaatacacc attatggaaa
      481 cgttgatacg caataatggc aaagtggtga gcaaagattc gttaatgctc caactctatc
      541 cggatgcgga gctgcgggaa agccatacca ttgatgtact gatgggacgt ctgcgcaaaa
      601 aaattcaggc acaatatccg caagaagtga ttactaccgt tcgcggccag ggctatttgt
      661 tcgaattgcg ctgatgaaaa aattactgcg tctttttttc ccgctctcgc tgcgggtacg
      721 ttttctgttg gcaacggctg cggtagtact ggtgctttcg cttgcctacg gaatggtcgc
      781 gctgatcggt tatagcgtca gtttcgataa aactacgttt cggctgttac gtggcgagag
      841 caatctgttc tatacccttg cgaagtggga aaacaataag ttgcatgtcg agttacccga
      901 aaatatcgac aagcaaagcc ccaccatgac gctaatttat gatgagaacg ggcagctttt
      961 atgggcgcaa cgtgacgtgc cctggctgat gaagatgatc cagcctgact ggctgaaatc
     1021 gaatggtttt catgaaattg aagcggatgt taacgatacc agcctcttgc tgagtggaga
     1081 tcattcgata cagcaacagt tgcaggaagt gcgggaagat gatgacgacg cggagatgac
     1141 ccactcggtg gcagtaaacg tctacccggc aacatcgcgg atgccaaaat taaccattgt
     1201 ggtggtggat accattccgg tggagctaaa aagttcctat atggtctgga gctggtttat
     1261 ctatgtgctc tcagccaatc tgctgttagt gatcccgctg ctgtgggtcg ccgcctggtg
     1321 gagtttacgc cccatcgaag ccctggcaaa agaagtccgc gaactggaag aacataaccg
     1381 cgaattgctc aatccagcca caacgcgaga actgaccagt ctggtacgaa acctgaaccg
     1441 attgttaaaa agtgaacgcg aacgttacga caaataccgt acgacgctca ccgacctgac
     1501 ccatagtctg aaaacgccac tggcggtgct gcaaagtacg ctgcgttctc tgcgtagtga
     1561 aaagatgagc gtcagtgatg ctgagccggt aatgctggag caaatcagcc gcatttcaca
     1621 gcaaattggc tactacctgc atcgtgccag tatgcgcggc gggacattgc tcagccgcga
     1681 gctgcatccg gtcgccccac tgctggacaa tctcacctca gcgctgaaca aagtgtatca
     1741 acgcaaaggg gtcaatatct ctctcgatat ttcgccagag atcagctttg tcggtgagca
     1801 gaacgatttt gtcgaggtga tgggcaacgt gctggataat gcctgtaaat attgcctcga
     1861 gtttgtcgaa atttctgcaa ggcaaaccga cgagcatctc tatattgtgg tcgaggatga
     1921 tggccccggt attccattaa gcaagcgaga ggtcattttc gaccgtggtc aacgggttga
     1981 tactttacgc cctgggcaag gtgtagggct ggcggtagcc cgcgaaatca ccgagcaata
     2041 tgagggtaaa atcgtcgccg gagagagcat gctgggcggt gcgcggatgg aggtgatttt
     2101 tggtcgccag cattctgcgc cgaaagatga ataaatatgt ccatacttca cgcattacgt
     2161 taagcatccg ttataatcgg ttgcagatac cagcctgtgg atgcttaaca tggaatacca
     2221 actcactctt aactggcccg attttcttga acgtcactgg cagaaacgcc cggtggtgtt
     2281 aaaacgcggc tttaataatt ttattgaccc gatctctcca gacgagttgg cgggtctggc
     2341 gatggaaagc gaagttgaca gtcgactggt cagtcaccag gatggcaaat ggcaggtcag
     2401 ccacggcccg ttcgaaagct acgatcatct cggtgaaacc aactggtcat tactggtaca
     2461 ggcagtgaac cactggcatg agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc
     2521 ggactggcgt attgatgatc tgatgatttc tttttctgta cctggcggcg gcgtcggccc
     2581 gcatctcgat cagtacgacg tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt
     2641 gggcgaaaag ctgcaaatga aacagcactg cccacatccg gatctgttac aggtcgatcc
     2701 gttcgaagcc atcatcgatg aagagctgga gcctggtgat attctttata ttccgccagg
     2761 attcccgcat gaaggctacg cgctggaaaa tgcgatgaac tattccgtgg gctttcgcgc
     2821 gccaaatacg cgggaactga ttagtggatt tgccgattat gtgctgcaac gtgaactggg
     2881 cggcaactac tacagcgatc ctgatgttcc acctcgcgct catcctgcgg atgttctgcc
     2941 tcaagagatg gataaactgc gtgagatgat gctcgaattg atcaaccagc cggaacactt
     3001 taagcaatgg tttggcgagt ttatatccca gtcacgtcat gaactggata tcgcgccgcc
     3061 agaaccgcct tatcagccag atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt
     3121 gcgcctgggt ggtctgcgcg tattgcgcat tggcgacgac gtgtatgcca atggtgagaa
     3181 gatcgattcc ccgcaccgtc cggcactgga tgcactcgcc agcaacattg cgctgactgc
     3241 ggagaatttt ggcgatgcgc tggaagatcc gtcattcctc gcgatgctcg cggcgctggt
     3301 caatagcggg tattggttct tcgaagggta agtttggatt taagccggat gcggcatcac
     3361 atgccgcatc ccttttcgct tacttccgtt gcgccgttaa ctcggcaata cggacgatca
     3421 cctgcaccgc tttttccata ccttccagag tcacaaactc atgcttacca tgatagttgt
     3481 aaccgccagt gaacaagttc gggcacggta atcccataaa cgacaactgc gcgccgtcgg
     3541 taccaccgcg gatcggtttc agttccggtt caatatcgca gtcacgcatc gcctgctggg
     3601 cgatatcgag aatatgcgga tgctcaacca ctttctctcg catattgtag taactgtctt
     3661 caatcaccag ttcaatgtag caatcaggat gtaacccttt gcccactttt ttggcgatct
     3721 ccatcatttt acgtttacgc gcttcaaact gtttacggtc gaaatcacgg atgatgtagt
     3781 gcatatcggc ccgttcaacg gtgcctttca tgctcgccag gtgatagaaa ccttcatagc
     3841 cttctgtcat ttccgggctt tcatccgccg gaacttccgc atgaatacgt gccgccagcg
     3901 acagcgcatt taccatcact cctttcgccg tgcccggatg aacattgtta ccgacaattt
     3961 tgatattgac cgatgcggcg ttgaagtttt caaactccag ttcgcctacg ccaccaccgt
     4021 caacagtgta agcccagcgg gcatcgaagg catcaacatc aaaatgtttc gcccctttgc
     4081 ccacttcttc atccggggta aaggcgacgc gaatatcacc atgcggaatg tttttctgtt
     4141 gcaataccgc cagcgcggtc atgatttctg caatacctgc tttgtcatcg gcacctaaca
     4201 aggttttacc atcggtggta atcaacgtct gacccagtag ctgatgcagc accgggaaca
     4261 taaccggtga taaaacttca tcgccgatac ccagcgcaat atcgccaccg cgatagtttt
     4321 caacaatttg cggattaaca tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa
     4381 taaagccaat cgccgggata tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc
     4441 ccttctcact taaggtcaca ttgataagcc ccatttcttc gagctgctct ttcagcagat
     4501 gcaataactt ccattggcct tccgtgctgg gaacctgtct cacccctgct tttgattggg
     4561 tatccagaga tacgtagttc aaaaatcgct caagtagttt atccatgtag tcaccctcac
     4621 tttttgtgac aacattatta agaagcaaga aaagacaaat attgcgtcag gtcactttta
     4681 cccctgcaag cgggaatatt tatcagcatt accttaatga atataaagct aagccagtaa
     4741 ttcacaacaa agattggcga ttcaagcggt ttgccgtaga attaccgccc tcattaagag
     4801 tcaccaaggt ggttaaccac aaaccccgca tcggtaagcc atccgttgcg tttacatggg
     4861 acagagtaaa aaattgaata aacaaccgag ttcgctttca ccgctggtgc aattggcggg
     4921 aattcgcaaa tgctttgatg gtaaagaggt cattccccag ctggatctga ctatcaacaa
     4981 tggcgagttc ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct
     5041 gattgcaggt ctggaaactg ttgattccgg acgcatcatg ctggataacg aggacatcac
     5101 ccacgttccg gcggaaaacc gctatgtgaa cactgttttc caaagctacg cacttttccc
     5161 ccacatgacc gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc
     5221 tgaaattacg ccccgcgtga tggaagccct gcggatggtg caactggaaa ccttcgctca
     5281 acgtaaaccg catcagctct ctggtggtca acagcaacgc gtcgccattg ctcgcgcggt
     5341 ggttaacaag cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg attacaaact
     5401 gcgtaagcaa atgcagaacg agctgaaagc gttacagcgt aagcttggta ttaccttcgt
     5461 ctttgtcact cacgatcagg aagaagcgct caccatgtca gacaggattg tggtgatgcg
     5521 cgagggtcgt attgagcaag atggtacacc gcgcgaaatt tacgaagagc cgaaaaacct
     5581 gtttgttgcc ggcttcattg gcgaaatcaa tatgtttaac gccactgtca tcgaacgtct
     5641 cgacgagcag cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct acgttaactt
     5701 cgccgttgaa ccggggcaaa aactgcatgt tctgctgcgc ccggaagact tacgtgttga
     5761 agagattaac gacgacaacc acgctgaagg gctgattggt tacgttcgtg agcgtaacta
     5821 caaaggcatg acgctggagt cggttgttga actggaaaat ggcaagatgg tgatggtcag
     5881 cgaattcttc aatgaagacg accctgactt tgaccactct ctcgaccaaa aaatggccat
     5941 taattgggta gaaagctggg aggtcgtact ggctgatgaa gaacacaagt aagttccaga
     6001 atgtagtgat tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg cccaacctga
     6061 tgatcattgg caccagcttt ttgacccgcg acgacgccag tttcgtcaaa atggtcttta
     6121 cgctggataa ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc
     6181 tgaatatggc gctgatcgcc accctcgcct gcctggtgct gggctacccg tttgcctggt
     6241 ttctggcgaa gctgccacac aaggtgcgtc cgctgctgct gtttctgctg attgttccct
     6301 tctggaccaa ctcactgatt cgcatctacg ggctgaaaat tttcctcagc accaaaggct
     6361 atctcaacga gtttttgctc tggctgggcg ttatcgacac accaattcgt atcatgttca
     6421 cgcccagtgc ggtgattatc ggtctggttt acattctgct accgtttatg gtgatgccgc
     6481 tgtactccag tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc gatctcggtg
     6541 ccagcaagtt acagactttt atccgtatca ttattccact gacgatgccg ggaattattg
     6601 ccggatgtct gctggtgatg ctgccagcga tgggcctgtt ctatgtatcc gacctgatgg
     6661 gcggtgcgaa aaacctgctg atcggtaacg tcatcaaggt ccagttcctc aatattcgtg
     6721 actggccgtt tggtgcagcc accagcatta cgctgactat cgtaatgggc ctgatgttgc
     6781 tggtttactg gcgcgcttct cgtctgctga ataaaaaggt ggaactcgaa tgatcggtcg
     6841 actgcttcgc ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc caatcattat
     6901 tttgattgtg aactccttta acagctcgcg ctttggcatc aactggcagg gttttaccac
     6961 caaatggtat agcctgctga tgaacaacga cagcctgtta caggcagcgc agcattcact
     7021 aacaatggcg gtgttttcgg cgacatttgc tacgcttatc ggttcactga cggcagttgc
     7081 gctgtaccgt tatcgctttc gtggtaagcc gttcgttagc ggaatgctgt ttgtggtgat
     7141 gatgtcacca gatatcgtga tggcgatttc tctgctggtg ctgtttatgc tgctgggtat
     7201 tcagcttggc ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc cgtttgtggt
     7261 ggtgacggtg tattcgcgcc tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga
     7321 tctcggtgcc agtgaattta ccattctgcg aaaaatcatt ctgccactgg caatgccagc
     7381 ggtggcggcg ggctgggtgt taagctttac cctgtcgatg gacgatgtgg tagtttcttc
     7441 gttcgtcacc ggaccaagtt atgaaattct gccgttaaaa atttattcga tggtcaaagt
     7501 cggcgtatcg ccggaagtta acgcgctggc aaccatatta ctggtgctgt cgctggtgat
     7561 ggttattgcc agccagctta ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa
     7621 atgaaaaaat ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg catgagcgcc
     7681 gctcacgccg atgacaacaa cacgctgtat ttctacaact ggaccgagta cgtgccgcca
     7741 ggactgcttg aacagttcac caaagaaacc ggtattaagg ctatctattc gacttacgag
     7801 tcgaacgaaa ccatgtacgc caagctgaaa acgtacaaag acggtgccta tgacctggtg
     7861 gttccttcaa cctattacgt cgataaaatg cgtaaagaag ggatgatcca gaagatcgac
     7921 aagtcgaagt taacgaactt cagcaatctc gatccagaca tgctcaacaa gccgtttgac
     7981 ccgaataacg actactccat tccgtatatc tggggtgcga cggcgattgg cgttaacggt
     8041 gatgcggtgg atcctaaatc tgtcaccagt tgggcagatc tgtggaagcc agagtacaaa
     8101 ggtagcctgc tgctgaccga cgatgcccgt gaagtgttcc agatggcgct gcgcaaactg
     8161 ggctactccg gtaacaccac cgatccgaaa gagattgaag ctgcatataa cgagctgaaa
     8221 aaactgatgc caaacgtcgc agcgtttaac tccgataacc cggctaaccc gtacatggaa
     8281 ggcgaagtta acctcggcat gatctggaac ggctctgctt tcgttgcacg ccaggcgggt
     8341 acgccaattg acgtggtgtg gccgaaagaa ggcggcattt tctggatgga cagcctggcg
     8401 atcccggcaa atgccaaaaa caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc
     8461 ccggatgtgg caaaacaggt tgccgaaact atcggttatc caacgccaaa ccttgcggcg
     8521 cgtaagctgt taagcccgga agtggcgaac gataaaacgc tctacccgga tgctgaaacc
     8581 attaaaaatg gtgaatggca gaatgacgtt ggcgcagcca gcagcattta tgaagagtat
     8641 tatcagaagc tgaaagcagg acgttaattc agcaaacggg cggtggtatt accgcccgtt
     8701 ttttagttag caaggatgtg gctaatgagt caggacagta aagttttatc ccgctctttc
     8761 gtcggtattg tggtcccctt gtaggcctga taagcgtagc gcatcaggca atgcttcctg
     8821 ctttataatc ccttcagcaa cttttcaaca aactcaggca ccacctggct tgccgggccg
     8881 taatatttct cggcaaattc attaccgacc tgacttggtt cgagattcag ttccacggtg
     8941 tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg ccggataaac atgcccggaa
     9001 gtaccaatgg caatgaaaat atcagccatc gacaacgcca tataaatttc atccatgccg
     9061 agtggcattt cgccaaacca aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa
     9121 tggcatttat cttctggcgt aacgtctccg gtccagtcga gaacctgacc actttgcgaa
     9181 caacgaactt tcagcaattc cccatgcata tgaatgacat tggtattacc tgcgcgttca
     9241 tgcaggttgt ctatattctg cgttaccagc aaaaagcgat cgccgagggc gtcttgcagt
     9301 ttagccagcg caagatgcgc ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga
     9361 cgggcgttat aaaacgcttg caccagttca ggatcgcgat cgaaaccttc cggcgttgcc
     9421 acatcttcaa cccgatgttc ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct
     9481 gattccgcag aaattcctgc ccctgtcagt acgagtactc ttggtttttc cattgcttcc
     9541 ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac gctcgcgcag gcggcgttta
     9601 tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt
     9661 taatcggtta gatgtaggaa ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg
     9721 cgttcaatgc gcggaacacg agctacaggt aagagatgac gaggcagcct gtccgccagt
     9781 tgcgttgtga ttgccgggaa attcgataag ccaccaccaa tgacgaccag gtcagagtca
     9841 acgatggtca ggatatttcc cagacaaacc gctaataaat ccagataacg ctcaacgtgc
     9901 gcccttgcct gctcatcgcc ttgatcataa agcgcaataa tttcgggagc ctgcaacggt
     9961 tgatgataat agtgttgcca gagccacgca aagccccgac ctgatagata attttcgata
    10021 caaccaattt gaccacagcc gcagcggcgt aacgggaaat ccagccccat catggttaac
    10081 gcatcaaccg gcagacgcat atggccaaac tcgccggtaa tgtagctttt accggtaatc
    10141 ggtttaccgt tgaaaatcag cccgccgcca acgccggtgc cgagaattaa ccccatcacc
    10201 agtggatatt gcgtaaattc atcatcccag gcttctgaaa gggcaaaaca gttggcatcg
    10261 ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt cggcacgcag cggtttaccg
    10321 ctggcagcgg ggacattggc ggcatacagc gtaccatctt ctgtttccgg cattcccgga
    10381 ataccgatgc caacagaacc tttacagcca aaacgttggt cagcttcggc taccagctcg
    10441 catactgcat ctaaaaatgc gtcatagctg tcacgcggtg tcggcacccg cttttcccac
    10501 tgcaactgcc gaccgctatc aaacacgcca agcgcaattt ttgttccacc aatatcaaac
    10561 ccgtaataca ttgccgctcc ttgttttaat gtactgcctt tactggccgc taaggactcg
    10621 cgcagggtca atattactgg cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa
    10681 caatgctgtg accagtacgt agaagacgtc cagccagtgc aattccgatg gcaggaagtc
    10741 aataaaatag atatcgctgg agaggaactg atgaccgatc agcttttcaa tccgctcaat
    10801 aatcggggta agttgcagtg aaacaactac gccgataatc acaccacaca ggctaccgaa
    10861 cagccctgcc agcaatccat accagacaaa gatggcgcga attaaaccat ctttcgcccc
    10921 cagcgttctt aatactgcga tatcgccact cttgtctttc accgccatca ctaaggtgga
    10981 gacgatgttg aaacaggcca cgccaatcac cagtaccatc gccagataca taatggcgcg
    11041 gatcatctgg atatcgcgat acatatagcc gtaagtacca atccaacttt taatataaac
    11101 atagctgttg gtcacttcac ccgcatcgcg taccagctta ttggcgttga aaacatccgt
    11161 cattttaagg gcaatacctg acacgctgga acccatatca agatattgtt gggcatccgc
    11221 cagcgggatc atggcaaaac tgtgatcgag ttgaccactc aactgcaaaa taccggcaat
    11281 gtgcaaacgc acacgttttg gctgcatcag tttatgctcg ggattcgagt tgggaatcat
    11341 gatcgacacc caatcgccct gcttcacttt cagcgcatcc gccacgcctt tgccgatgat
    11401 aatttgctgt tcgcccgctt taaaattgcg ccaggcatcc ccctgaacaa acgagggtaa
    11461 tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc acctgaattg cgcgcagatt
    11521 ggtaccactt tctaccagtc cggtgaaatt gatatacggc gcagcggcgg caatacccgg
    11581 cactttttgc acgttatcca gtgcttcctg ccagttagtc catggctgat ttaccgcctc
    11641 aatttcaccg tgcggtacca cagcaagaat gcggttattc agttcgcgtt caaagccgtt
    11701 catcgcgctt aagccgacga tcaataccgc cacgccgagg gcaatgccaa tggtagaaat
    11761 gacagagatt agcgacacca tgccgccgcg tcgccgtccg cgactaaaac gcaggccaat
    11821 caataacgat aaaggcatcg ccattactcc gcccccatca ggctcagttc cgccgtcaga
    11881 cgtccatcac gcatttccag ttggcggctc atacgtttcg ccagttgcag gtcgtgagta
    11941 accaccagga aggcggtgcc ctgcaagcga ttcaattccc caagcaactg gaagatgctg
    12001 tcggcgttac gcgcatcgag gttaccggta ggctcatccg caagtaccag acgcggatta
    12061 ttgaccagcg cacgggcaat cgccacacgc tggcgttcgc cgccagaaag ttcagatggg
    12121 cggtgattcg cacgatgctc cagccccacc gcttttaaca tctccagcgc acggctgttg
    12181 atttcagcgg gctttttctt gccaatcagc agtggcatag ccacgttttc cagggcagta
    12241 aaatccggca gcaggtggtg aaactgataa ataaagccca gcttctggtt gcgcagttct
    12301 gctttcgccg ccgaagacag tttgctcatc ggttgaccgt taaagatcac atcgccggag
    12361 gttggtgtat ccagcccgcc cagcaggtgc agcaaggtac ttttaccgga accagagcta
    12421 ccgacgatcg ccatcatttc accttcgccg acgctgaaac tgacattgtg cagtacatcg
    12481 gtttgcacac tgccttcctg atagcgtttg cacaggttgt cgcattgcaa caggatctta
    12541 ttcataacgt aaagcctcag cgggttgagt ggcggcagcg cgccatgaag ggtaaagcgt
    12601 agacagcagc gcgatagcca tcgccaccag cgcaataaca atgacctgta aaggttcgat
    12661 agcgaccggc agcgccgcgc catcaagcag gacgccgatt atcggcatca gattatttaa
    12721 ctggctggca agcagtgcgc caagcgccgc tccgaggatc gcaccgataa tcccggcgct
    12781 ggccccttgc accataaaga ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag
    12841 gatcgcgact tcgccctgct tctccattac catcagccct agtgaggtaa taatgttaaa
    12901 cgccgcaacg gcgacaatca ggctcagcag caagcccatc atattttttt ccatgcgtac
    12961 ggcctggaac agctcgcctt tacggtcacg ccagtcctgc catttgctgc cttcaggcag
    13021 tttttgctga cttaaagagt caactttcag cggctcatcc agccacaaac gccagccggt
    13081 aatattgcct gccggataac gcatcagacg cgaggcatcc tcaatattca ccagcatttc
    13141 atagccatcg acttcactgt tggcggcgaa attgccaatc acattgaaca ggcgctggct
    13201 tggaatacgc cccatcggcg tgaactggct ggcagatggt accatcacgc ggatttgatc
    13261 accgcgatta acgcctagct gtgaggcaag ttgttcgccg aggatgacat tatatttccc
    13321 cggctcgagg tcagtttgtt tcacattgac cagatacggc gtaagtggat ctttttgcgc
    13381 cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc
    13441 accggtagta ataggtgcga cgcgattaac gccgtccagt ttgactgccg tttccgggag
    13501 ttgctgcggg ttaagagagc catgctcaga agagagaatt gcctgtggca tcaggccaag
    13561 gatgttgttt tgcagctcgc gctcaaagcc gttcatcact gacaatactg tgaccagcgc
    13621 catcaccccg agggtaatgc cgatggtaga aagccaggag acgaaacgac cgaagcgatc
    13681 cgctgcacgc ccacgcatgt aacgcaggcc aataaataga gcgacaggtt ggtacatgaa
    13741 atccgtctgg ttgctgtagc aaagacacga gtatataagc gaaagcaaga tgggtaaacg
    13801 tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa
    13861 attgccatgt tttatcatca atcttgccgc ctgatcttaa tctgaaaaat catccaatac
    13921 agacagatac ccattacacc tcgggtcata acccgcagga tcaaccgcat gtaaattaaa
    13981 aaagagcgtg ttgtcctgat gaaacaaaaa gagctatgga ttaaccagat caaagggtta
    14041 tgtatttgtc tggtggtgat ttatcactcg gtcattacct tttatccgca tatgaccact
    14101 tttcagcatc cgttatcgga agtcctgagt aaatgctgga tctatttcaa tctttacctt
    14161 gcaccctttc gtatgccggt ttttttcttt atatccggct atttgattcg ccgctatatc
    14221 gacagcgtac cgtggggaaa ttgtctcgat aaacggatct ggaacatctt ctgggtgctg
    14281 gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct
    14341 gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg attccaccgg tgagttcctg
    14401 cacgggatga tcaccgccag caccagcttg tggtatctgt atgcgttaat tgtctatttc
    14461 gtggtatgta aaatttttag ccgtctggcg ctgccactat tcgccttgtt tgtactgctg
    14521 agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa tgaacagtgt gatccgcaat
    14581 ttgccttatt acagccttgg cgcatggttt ggcgcaacaa taatgacctg tgttaaagag
    14641 gtgccgttgc gccgccatct gctgatggct tctttgctta ccgttctggc ggtcggtgcc
    14701 tggttgttta ctatctcgct gctgttgtcg ctggtatcga ttgtggtgat catgaagctg
    14761 ttttatcagt acgagcaacg attcggtatg cgctccacca gcctgctgaa tgtgattggc
    14821 tccaacacca ttgctatcta caccacccat cgcattctgg ttgaaatatt cagcttaact
    14881 ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag tcgaactgac actcctgctg
    14941 gtttatccct ttgtcagttt gttcatctgt actgttgcgg gcttgctggt aagaaaactt
    15001 tcacagcgtg cattcagcga tctgttgttc tccccgcctt cactgcccgc tgccgtcagt
    15061 tactctcgtt aaaccatcgc cccagccggg gcgattcaat ttgctaaacc atgtcgatta
    15121 cggataatga acagcattgc ttatcagaga tatgccccca tatgttgagg catatcctaa
    15181 cgagaatctg acaaccgtta tgcctgaaca atatcgttat acactgcccg tcaaagcggg
    15241 tgagcagcgt ctgctgggcg agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat
    15301 tgccgaacgt cacgccggtc cggtggtact cattgcacca gatatgcaaa atgctctgcg
    15361 tttgcatgat gaaatcagcc agttcaccga tcagatggtg atgaatctgg cggactggga
    15421 aactcttccc tacgacagtt tttcgcctca tcaggacatt atctcctcgc gcctttccac
    15481 cctttaccag ctaccgacga tgcagcgtgg cgtactgatt gttccggtga atacgcttat
    15541 gcagcgcgtt tgcccacaca gttttctcca cggtcatgcg ctggtgatga aaaaaggtca
    15601 gcgcctgtca cgagatgcat tacgaaccca actggacagc gccggttatc gccatgttga
    15661 ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg ttgctggatc tcttcccgat
    15721 ggggagtgag ctgccttatc gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt
    15781 gtttgacgtc gacagccagc gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc
    15841 gcacgaattt ccgaccgata aagcggcaat tgaactgttc cgcagccagt ggcgcgatac
    15901 cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa gtgagtaaag gcacattacc
    15961 tgccgggatc gagtactggc agccattgtt cttcagcgaa ccactgccgc cgctgttcag
    16021 ttatttccct gccaatacct tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg
    16081 tttccaggct gacacgctgg cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct
    16141 gttgccacca caatcgctct ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg
    16201 gccccgggtg cagctaaaaa ctgaacattt accgacaaaa gccgcgaatg ccaatttagg
    16261 tttccagaaa ctgccagacc tggccgttca ggcacaacaa aaagcgccgc tggatgcgct
    16321 gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg
    16381 ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt gctccgcaac gcattatgcg
    16441 tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt ggcgctgccg aacatggttt
    16501 tgtcgataag gtgcgtaatc tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt
    16561 tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc gatacactga tccgtaacct
    16621 tgcggagctg catattggtc agccggtggt ccatctggag cacggtgtcg ggcgctatgc
    16681 cggaatgacc acgctcgaag cgggcggcat tactggcgag tatttgatgc tcacctatgc
    16741 caacgacgcc aaactgtatg ttccggtgtc gtcactgcat ctgattagcc gttacgcagg
    16801 tggcgcggaa gaaaacgccc cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg
    16861 gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca
    16921 acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt gagcagtatc agttgttctg
    16981 cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag gccattaatg cggtacttag
    17041 cgacatgtgt cagccgctgg caatggatcg tctggtgtgc ggcgatgttg gctttggtaa
    17101 aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat aaccacaagc aggtggcggt
    17161 gttggtgcct accacccttc tcgcgcagca gcattacgac aacttccgcg accgtttcgc
    17221 caactggccg gtacgtatcg aaatgatctc ccgtttccgc agcgccaaag agcagacgca
    17281 aatccttgcg gaagtggcgg aagggaaaat cgatattctg atcggtacgc acaaactgct
    17341 gcaaagtgac gtcaagttta aagatttagg cctgctgatt gtcgatgaag aacaccgctt
    17401 cggggtgcgt cataaagagc gcattaaagc gatgcgcgcg aacgtggata ttctgacgct
    17461 tactgcaacg ccgatcccac gcacgctgaa tatggcaatg agcggaatgc gtgacctgtc
    17521 gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga
    17581 cagcctggtg gtccgggagg cgatcctgcg tgaaattttg cgcggggggc aggtttatta
    17641 tctctacaat gatgtggaaa acatccagaa agctgccgaa cggctggcag aactggtgcc
    17701 agaagcgcgg atcgccatcg gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat
    17761 gaatgatttc catcatcaac gtttcaacgt gctggtttgt acaaccatta tcgaaaccgg
    17821 gatcgacatc ccgacagcca acaccattat cattgaacgc gcggatcact tcggtctggc
    17881 gcagctgcac cagttacgcg gtcgcgtcgg acgttcgcat catcaggcat atgcatggtt
    17941 gctgacaccg catccaaaag cgatgactac cgatgcacaa aaacgtcttg aagcgattgc
    18001 ctcgctggaa gatctcgggg caggttttgc gctggcaacg cacgatctgg agattcgcgg
    18061 cgcgggtgaa ctgcttggcg aagaacaaag cggctcaatg gaaaccatcg gtttctcgct
    18121 gtatatggag ttgctggaaa acgccgtcga tgcactgaaa gccggacgcg agccgtcgct
    18181 ggaagatctc accagccagc aaacagaagt cgagctgcgg atgccgtcgc tattgccaga
    18241 tgatttcatt cctgacgtga atacgcgttt gtcgttctat aaacgtattg ccagcgccaa
    18301 aacggaaaac gaactggaag agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc
    18361 agatccggcg cgtaccctgc tggatattgc ccgtctgcgc cagcaagcgc agaaactggg
    18421 gatcaggaag ctggaaggta atgagaaagg cggggtgatc gaatttgccg agaagaatca
    18481 cgttaatccg gcctggttga ttggtttgct gcaaaaacag ccgcagcatt accgccttga
    18541 tggtccgacg cgcctgaaat ttattcagga tttgagtgag cggaaaacgc gtatcgaatg
    18601 ggtacgccag tttatgcgtg aactggaaga gaacgcgatc gcttaatggt aaatcggggg
    18661 cgtttctgcg cccccatctg caacatttac aaattctttg cacttccctg cactatccga
    18721 cactgtcacc atccataatt caggcttatc tgtttattac aataacctta tatttattat
    18781 tgatttttgg tgatgatcaa aacgcgtttt tctcgctggc taacgttttt tacgttcgcc
    18841 gctgccgtgg cgctaccggc aaaagccaac acctagccgc tgccgccagc gagcagtcgt
    18901 ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct ggaagccatc
    18961 gccaaaaaat ataacgtcgg ctttctcgct ctgttacagg ctaaccccgg cgttgatcct
    19021 tacgtaccgc gcgcgggcag cgtgttaacg atcccgttgc aaaccctact tccagacgcg
    19081 ccgcgcgaag gcattgtgat caacattgcg gagctgcgtc tctattacta cccgccgggt
    19141 aaaaattcgg taaccgtgta tccaataggt attggtcagt taggtggtga cacgctgaca
    19201 ccgacaatgg tgaccaccgt ttcagacaaa cgtgcaaacc caacctggac gccaacggca
    19261 aacatccgcg cccgttataa agcacaggga attgagttgc ctacggtagt gccggctgga
    19321 ccggataacc caatgggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg
    19381 cttcacggta cgaacgccga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt
    19441 ctgcgggatg acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat
    19501 atcatcaaca ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta
    19561 catcagccgc tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat
    19621 agcgcaatgc aatcatttaa agatgcagca caaactgacg ctgaagtgat gcaacatgtg
    19681 atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact
    19741 ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga aaaaatcagc
    19801 agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg
    19861 tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt
    19921 gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg atagtaccga
    19981 ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagttg gcaaatgaca
    20041 tggagcttaa aatcgccgca gcgatgaggg tttttacgtt tttcataata gtggccttat
    20101 gcagatgaat gacgtcgcaa gaaattactt aacgatcgat aacta
//
LOCUS       DAZOYL010000046        20004 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZOYL010000046 DAZOYL010000000
VERSION     DAZOYL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.046"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..70
                     /locus_tag="ACHUOY_001217"
     CDS             <1..70
                     /locus_tag="ACHUOY_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFR0682046.1"
                     /translation="ILKTTDYLRIAQPELLNFRRTA"
     gene            complement(71..175)
                     /gene="yoaI"
                     /locus_tag="ACHUOY_001218"
     CDS             complement(71..175)
                     /gene="yoaI"
                     /locus_tag="ACHUOY_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR0682047.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            345..791
                     /locus_tag="ACHUOY_001219"
     CDS             345..791
                     /locus_tag="ACHUOY_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR0682048.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(748..1569)
                     /gene="nimR"
                     /locus_tag="ACHUOY_001220"
     CDS             complement(748..1569)
                     /gene="nimR"
                     /locus_tag="ACHUOY_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR0682049.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            1666..2847
                     /gene="nimT"
                     /locus_tag="ACHUOY_001221"
     CDS             1666..2847
                     /gene="nimT"
                     /locus_tag="ACHUOY_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR0682050.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            2902..3249
                     /locus_tag="ACHUOY_001222"
     CDS             2902..3249
                     /locus_tag="ACHUOY_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR0682051.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3271..3525)
                     /gene="yoaF"
                     /locus_tag="ACHUOY_001223"
     CDS             complement(3271..3525)
                     /gene="yoaF"
                     /locus_tag="ACHUOY_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR0682052.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            3708..4733
                     /gene="dgcP"
                     /locus_tag="ACHUOY_001224"
     CDS             3708..4733
                     /gene="dgcP"
                     /locus_tag="ACHUOY_001224"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR0682053.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            4766..4864
                     /locus_tag="ACHUOY_001225"
     CDS             4766..4864
                     /locus_tag="ACHUOY_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR0682054.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            4867..4941
                     /gene="yoaJ"
                     /locus_tag="ACHUOY_001226"
     CDS             4867..4941
                     /gene="yoaJ"
                     /locus_tag="ACHUOY_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR0682055.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(5000..5248)
                     /locus_tag="ACHUOY_001227"
     CDS             complement(5000..5248)
                     /locus_tag="ACHUOY_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0682056.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(5396..5578)
                     /locus_tag="ACHUOY_001228"
     CDS             complement(5396..5578)
                     /locus_tag="ACHUOY_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR0682057.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(5582..5941)
                     /gene="yeaR"
                     /locus_tag="ACHUOY_001229"
     CDS             complement(5582..5941)
                     /gene="yeaR"
                     /locus_tag="ACHUOY_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR0682058.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(6114..6752)
                     /gene="leuE"
                     /locus_tag="ACHUOY_001230"
     CDS             complement(6114..6752)
                     /gene="leuE"
                     /locus_tag="ACHUOY_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR0682059.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(6879..7802)
                     /gene="dmlR"
                     /locus_tag="ACHUOY_001231"
     CDS             complement(6879..7802)
                     /gene="dmlR"
                     /locus_tag="ACHUOY_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR0682060.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            7905..8990
                     /gene="dmlA"
                     /locus_tag="ACHUOY_001232"
     CDS             7905..8990
                     /gene="dmlA"
                     /locus_tag="ACHUOY_001232"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR0682061.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVAIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            9241..10851
                     /gene="yeaV"
                     /locus_tag="ACHUOY_001233"
     CDS             9241..10851
                     /gene="yeaV"
                     /locus_tag="ACHUOY_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR0682062.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            10883..12007
                     /gene="yeaW"
                     /locus_tag="ACHUOY_001234"
     CDS             10883..12007
                     /gene="yeaW"
                     /locus_tag="ACHUOY_001234"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR0682063.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            12063..13028
                     /gene="yeaX"
                     /locus_tag="ACHUOY_001235"
     CDS             12063..13028
                     /gene="yeaX"
                     /locus_tag="ACHUOY_001235"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR0682064.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(13082..14197)
                     /gene="rnd"
                     /locus_tag="ACHUOY_001236"
     CDS             complement(13082..14197)
                     /gene="rnd"
                     /locus_tag="ACHUOY_001236"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR0682065.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(14279..15964)
                     /gene="fadD"
                     /locus_tag="ACHUOY_001237"
     CDS             complement(14279..15964)
                     /gene="fadD"
                     /locus_tag="ACHUOY_001237"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR0682066.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(16169..16750)
                     /gene="yeaY"
                     /locus_tag="ACHUOY_001238"
     CDS             complement(16169..16750)
                     /gene="yeaY"
                     /locus_tag="ACHUOY_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR0682067.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(16790..17485)
                     /gene="tsaB"
                     /locus_tag="ACHUOY_001239"
     CDS             complement(16790..17485)
                     /gene="tsaB"
                     /locus_tag="ACHUOY_001239"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR0682068.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(17543..19453)
                     /locus_tag="ACHUOY_001240"
     CDS             complement(17543..19453)
                     /locus_tag="ACHUOY_001240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR0682069.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            19585..19929
                     /locus_tag="ACHUOY_001241"
     CDS             19585..19929
                     /locus_tag="ACHUOY_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0682070.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4849 a         5190 c         4908 g         5057 t
ORIGIN      
        1 tattttgaaa accacggatt atttgcgcat tgcgcaacca gaactgctca atttccgccg
       61 cactgcgtaa ctagcctgtc cgttcaataa gcagaacgga caaaaccagc acaacagcaa
      121 tggcaaaaaa cgatgacgtg ataatcagtg tctcgacgaa catttgatcg ttcatagcat
      181 gcccccggga gatagctgtt ttcacgttat tgttagcgtg cacaaatggc agtttgatga
      241 caattcgcct gattttttat gcaaaaaacg taaagatttt ctacttcctt cctgcagcaa
      301 acgtaaagta agcaggctta ttattttttg gcaaggaaac cacgatgttt gatgtcactt
      361 tgctgatcct gctcgggtta gctgcgctgg gctttatcag tcataacacc actgtcgccg
      421 tttcaattct ggtgttgatc attgtccgcg tcacaccgtt aagcaccttt ttcccgtgga
      481 ttgaaaaaca ggggctaagt atcgggataa tcattctgac gattggcgtt atggccccca
      541 tcgccagcgg gacgctaccg ccctcaacct tgattcactc gtttctgaac tggaaatcgc
      601 tggtggcaat tgcagtaggc gtgattgtct cctggctggg tgggcgcggc gtgaccttaa
      661 tgggcagcca gccgcaactg gtcgccgggc tgctggtagg cactgtttta ggcgtggcgc
      721 tgtttcgcgg cgtaccggtc ggcccgctta ttgccgcggg tctggtttcg ctgattgtgg
      781 ggaaacagta gttaatcccg caatatatcg gcctggcgtt tgccccaggc ctttcttaaa
      841 catagtaata aaggccgttg ttgaatcata ccccagcgta tgagcaactt tctgtacagt
      901 atcgcctttc actaatccct gaagtgccat aatcagctgt aactgctgac gccactggcg
      961 aaaactcaac cccgtctctt ttactatcag tcgcgcaaga ttgcgttcac tcatcgcaaa
     1021 aaagccagcc cattgcccta atgaccccca ctcgacgggc cctttcgcca tcatctccac
     1081 catcgtgcgg attttaggat gagaagaaac gggcaggtgc agctgttgtt gtggctgctg
     1141 agggagttca tcaaacagga cctgaattaa tctgcgggtc atcggctcag cacgctgagt
     1201 cgtcgtcctg tttgccaggg ttaaaatcaa ttcccggcat aagggcgata ttttcaatgt
     1261 gcaacatgtt gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc
     1321 gttagctgtc acttgattgc tatgttccac cccgcccggt atccaaaccg catattgcgg
     1381 cggcaccatc cataaagcat tttccaccgt acaggtaatt gcaccatgta atgctagtat
     1441 cagctgtcct ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat
     1501 acaaaatgcg accgcggctt catggtgtcg gtcgggttca tagccattga gattcaacct
     1561 gtgcatcatt ttgtccgaat ttagcgataa tttgtcattt tagcttgatt caacataaca
     1621 ataaaaacgg taaggtacag cctcgtttgt aacaatgaga agcatatgac ctgttcaact
     1681 tcattaagcg gcaaaaacag gattgtcctt atcgctggca ttctgatgat tgccacaaca
     1741 ttacgcgtca cctttatcgg cgcagcaccg ttactggata cgattcgttc cgcttactcg
     1801 ctgacgacag cgcaaaccgg cttattgacc accctgccat tattggcctt tgcgctaatc
     1861 tcacctttgg ccaccccggt agcgcgacgt tttggtatgg aacgtagcct gtttgccgcg
     1921 ttacttttga tctgtgctgg tatcgcaatt cgctctctcc cttcgcctta cttattattt
     1981 ggcggtacag cggtcattgg cggtgggatt gcattaggca atgtcttact gccaggatta
     2041 attaaacgcg atttccctca ttccgtcgcc agacttaccg gcgcatattc cctgacaatg
     2101 ggagctgcag cggcactggg atcggctatg atagtgccgc tggctttgaa cggttttggc
     2161 tggcaaggcg cgttgctcat gctgatgtgt tttcctctgc tggctctttt tttatggctg
     2221 ccacagtggc gaagccaaca acatgcaaat ttgagtacct cgcgcgcctt acatactcgg
     2281 ggtatctggc gttcgccgct tgcctggcag gtaacattgt ttcttgggat caactcactg
     2341 atctattacg tgattattgg ctggcttccg gcgatcctca tcagtcacgg ctatagcgaa
     2401 gcacaggcgg gttcactgca tggtttgctg caactagcca cagcagctcc cggtttgctg
     2461 atccctcttt tcttacatca tgtgaaagat cagcgtggta ttgcagcgtt cgttgccttg
     2521 atgtgcgcag tgggcgctgt tgggctctgc tttatgccag cgcacgcgat cacctggact
     2581 ctgcttttcg gttttggttc cggcgcaaca atgatactgg ggttgacgtt cattggtctt
     2641 cgggcaagtt ctgcgcatca ggcggcggca ctctcgggga tggcacaatc cgtcgggtat
     2701 ttgctggcag cctgtgggcc gccgctaatg ggtaaaatac acgatgctaa cggtgactgg
     2761 tccgtaccac tgatgggtgt tgcaatactt tcactactga tggcgatttt cggactttgc
     2821 gccgggagag acaaagaaat tcgctaatat ccggtgctat agtgacgtga caaatcatgc
     2881 gtgaaaggga gaacaaacac gatgaatatt cagtgcaaac gcgtttatga tccggctgaa
     2941 cagagcgatg gttatcgcgt actggtcgac cgcctctggc cgcgcggtat caaaaaaacc
     3001 gatttagccc ttgatgagtg ggataaagaa atcacgccgt caacggaact gcgcaaagcc
     3061 tttcacggcg aagtcgtcga ttttgcaacc tttcgcgagc aatatcttgc agaactggcg
     3121 caacacgagc aagaaggaaa gcggctggcg gacatcgcca aaaaacagcc gctgaccctg
     3181 ctctacgcag caaaaaacac cacgcagaac catgcgctgg tgctggccga ctggctacgt
     3241 agcttgtgat tttagtacag catccggcgg ttatttttca ccagccggat ggtcccgtcg
     3301 ccacaatgcc cattcatcaa tcgtttcacc gcccggtaat ttgcaattgt tgctgacccc
     3361 ttgcgctgtc gacactggaa tgagcgtccc gcccttctgc tggcaataga ccgacgccgg
     3421 atttgccata ccaatctgcg gcggtttagg tacttctggc tgagaagagg ttgaacaacc
     3481 agccaggacc agcaagcaag gcaaagcaaa actgataatt ttcatttatt gatctcactc
     3541 atttatctac gcttagagta tcgcggtatc gttttgtttt gcagcactat ttttattaca
     3601 ttcactcaaa acatattacg tcttgtttca tctttgttgg tgatgtttta tcatgcctgc
     3661 aaagattaaa taatcagcat ttacccgccg tatcctggag ttgtttcgtg tcagatcaga
     3721 ttatcgcccg cgtctcgcaa tcccttgcca aagaacagtc actggaaagt ctggtccgac
     3781 agcttctgga gatgctggaa atggtcactg atatggaatc aacctacctg accaaagtgg
     3841 atgtcgaagc gcgcctgcag catattatgt ttgcccgtaa cagccagaaa atgcacatcc
     3901 cggagaattt taccgtctcg tgggattact cgttatgcaa acgcgccatt gatgaaaact
     3961 gctttttcag cgatgaagtc cctgaccgtt ggggcgactg tattgcggca cgcaatcttg
     4021 gcatctccac atttctgagc acaccaattc acttaccgga tggatcattc tatggcacgc
     4081 tttgcgccgc cagcagtgag aagcgccagt ggagtgaacg cgcggaacag gttttacagt
     4141 tattcgccgg actgattgca caatatattc aaaaagaggc actggttgaa cagttgcgcg
     4201 aagccaatgc tgcgctgatt gcgcaatcgt ataccgactc gttaaccggg ttgccgaatc
     4261 cgcgggcgat ttttgaaaat ctgacgacgc tgttttctct cgcccggcat cttaaccata
     4321 agataatgat cgcgtttatc gatctggata acttcaaatt aatcaatgat cgttttggtc
     4381 ataatagtgg cgatctgttt ctcattcagg ttggcgagcg ccttaatacg ctccagcaaa
     4441 atggcgaagt tattggtcgt ctcggcggtg atgagttttt ggttgtttca ctaaacaacg
     4501 agaatgcgga tatttcgtcg ctgcgagaac gcattcagca gcaaatacgt ggagaatatc
     4561 acttaggtga tgttgatttg tattatcccg gtgccagtct tggcatagta gaagtcgatc
     4621 ctgaaacaac cgatgcagac agtgccctgc atgctgccga tattgcgatg tatcaggaga
     4681 aaaaacacaa acagaaaaca ccttttgtcg cgcattcagc gccacattgc tgaggcgtat
     4741 tcacatcctt ttgattggtg ataacatgcg aatcggtatt atttttccgg ttgtaatctt
     4801 tattacagcg gtcgtatttt tagcatggtt ttttattggc ggctatgctg ccccgggggc
     4861 ataaagatga aaaaaacaac gattattatg atgggtgtgg cgattattgt cgtactcggc
     4921 actgagctgg gatggtggta acgtcacctc taaaaaatag caaaggctgc ctgtgtgcag
     4981 cctttgtgca atttaagcgt taacttttaa tcttcctgta gataaatagc acgacaatcg
     5041 caccaataac ggcaaccacg aagctgccaa aattgaagcc atcgacttta ccaaagccaa
     5101 acagcgtgct gatccatccg ccgactacgg caccgactat ccccagcagg atagtcataa
     5161 agaatccacc accatcttta cctggcatga tccacttcgc cagaataccg gcaataagcc
     5221 caaaaataat ccatgacaga atgcccattg tttcctcact tatctgtttt gcattagcgg
     5281 gttagtcgct gataaaaagc atagcacaac atcgggaggg caagatttgt gacgagcatc
     5341 acggaggatt tttttgcgat ggcgcagaaa ttgcgccatc aacgatcagt gataattacc
     5401 aaccacaaac atcatgttcg ttttccgtgt cataagaacg cacggtattc accagatctt
     5461 ttatcacttc agccgccact tctggcacca gcaaagtcat cggcgtctct gtttcataat
     5521 cgacagaaac gccattactg ttattggtga cggtcacggt atacgttgct ttgcccatga
     5581 ttcatttccc gttatgaatg actttccgtt gttgcgcacc ttccatcagg acttcaggag
     5641 ccacgaagaa gtcaatgttg aaataagtat cgtcagtcat ggcttcaatg ttgtgccact
     5701 tttctggagg gaacacctca aactgccccg cttcgataag gatcacctga tcaggctctg
     5761 cactgtgttc atcagcgtag ccgagatatt tgaccgcccc atgcataacg gaaaggcgtg
     5821 ggtaaacccc cgggcgcgtt cctttatcaa gatgacgttc gaatattccg gcaggcgcag
     5881 tttgtttatt ccagaaaggc gttgagcgcg tatgaatata attctgtggg atttgcagca
     5941 tccttttccc tccttcggtg aatgcgctga aaacggttta ttccagccgt ttcagggtac
     6001 gcctgataat ttgcatttta aataccattt attggttact ttttagcacc atatcagaga
     6061 aaaatcaggg aggattacaa gtgggaagcc cgcgacaacg cgggctgaat gtatcaggat
     6121 tgcagcgtcg ccagtcgggc ggcgaaaccc acgaacatca aaccaatcag tgagttgcca
     6181 actttagcca gtttcttttt ggtacgtatg tactgcgtga caaaagcacc agaaataatc
     6241 aggaagctca aatagcagaa actcaccagt tccagcgtcg tcgccagaat aaagaatgaa
     6301 attcccgtat gtggggcatt aacatcgata aactgtacga aaaacgacac atagaacaaa
     6361 atggctttcg gattagtcag gctcaaaatt aacgcgcgtt taaaaatggc cccgtattgg
     6421 ggctcatcgg atttggcctc gttattttta cccttcaggg tcgcgtagag aattttactc
     6481 cccagataga gcaaataaaa cgcaccaaga taacgtacaa tgttgaataa tatcggggtg
     6541 gtcttaatta atgtcgccac tccagcccat gccagaaaca tcaatactgc atcgccaata
     6601 aatacaccgc aggccgcaag ataaccgcct ttcataccgc tactgacgct atttttgagt
     6661 acaaacaggg tatttggccc tggcaccaac acaataaaaa tggccccaac cagataggtc
     6721 cagtaattca gaaccccgta ttcagcgaac acattaacct ctttaattat ctttcgatca
     6781 tgcgcgatta aaggtgaata tgctaaccaa tctgtagcgg cttagaaagg agaaaatcag
     6841 gttttaaccc gatatcaacc cgataattga ataattaatc aggcatgctc cagtgaaaaa
     6901 ttcgggtagt gctcggcaaa atactggcgt aaaaattcta tcgttatccg cactttcgct
     6961 gacgtcgcca gccttgaaac ataaacggcc cagacgttcg ctggctggta atattccggt
     7021 agcacttgca ctaaatgacc actggcaatg ttttcgctaa catcccacca ggagcgcaga
     7081 gcaatcccct gcccatcgag acaccactga tggacaattt ctccatgatt agacgacagt
     7141 gggccggtga ctttaatcgc atggtggcct tctttattgc gtaattgcca gacaccaaaa
     7201 ggatgatcgc gctctttaat gaccaggcac ggcaatgcgg aaagatcggt taaatgtttc
     7261 ggcgcgccat gttgcgcaat aaattccggc gacgcgcaga gaatgcggta attagtcgcc
     7321 agtttgcggg cgattaaatt aggggcgatg tcatcgccaa tgcgaatatc gagatcaaca
     7381 ccttcattga ccaaatcgac caaccggtct tccacatcaa aacgtaattc cagttgcgga
     7441 tacgccttag ccagcgctga taacgccgga gccaccacct gtcgtccaaa accaaagctg
     7501 ctgataatac gcagcatccc ctgcggcacc tgacgcacgt cagaaagttc gtccatcatc
     7561 tgaccgacat cctgcaaaat ccgctgcgcc cattcataaa tccgctctcc ttcttcggta
     7621 atggtgacgc ggcgggtggt gcggtgtaac aacaccacgt ttagcgtttg ctccagcaag
     7681 gcgatgcgct tgctgacgaa cgccggtgaa acgcccagtt cttccgccac ggcggcaaaa
     7741 ccagcccggc gagccaccag cataaagacg cgcaaatcgt tcagcagcgg taaattattc
     7801 atgattcgtg ttttatgttt caccagttac ggggattaat tccttttcag tcaattatag
     7861 gatggtgatg ttgtcaattt tgatggtcag gaagtgagaa cccaatgatg aaaacgatgc
     7921 gtattgctgc gatcccggga gatgggattg gcaaagaagt ccttcctgaa gggattcgcg
     7981 tattacaggc tgccgctgag cgctggggct tcgacttgag ttttgagcaa atggagtggg
     8041 cgagctgcga gtattacagc catcacggta aaatgatgcc ggacgactgg catgagcaac
     8101 ttagccgttt cgacgccatc tattttggtg ccgtcggctg gccggatacc gttccggacc
     8161 atatttcgtt gtggggttcg ctgctgaaat ttcgtcgtga attcgaccag tacgtcaacc
     8221 tgcgcccggt tcgtctcttt cctggcgttc cctgcccgct ggcgggaaaa cagcctggcg
     8281 acatcgattt ttacgtggtc agggaaaaca ccgaaggcga atattcctcg ctcggcggta
     8341 gagtgaatga aggtacagag catgaagtcg ccattcagga atcggtattt acgcgtcgtg
     8401 gtgtcgatcg cattttgcgt tatgccttcg aacttgcgca aagccgccca cgtaagacgc
     8461 taacttctgc cactaaatca aacggtttag ccatcagcat gccgtactgg gatgagcgag
     8521 tggaagcaat ggccgagaat tacccggaga tccgctggga caagcagcat attgatattc
     8581 tctgcgcgcg ttttgtgatg cagccggaac gcttcgatgt ggtggtggcg tccaatttgt
     8641 ttggcgatat cctttccgat cttggcccgg cctgcaccgg caccattggc attgccccat
     8701 ccgccaacct gaatccggaa cgcactttcc cgtcgctctt cgagcctgtc cacggttccg
     8761 cgccggatat ctacgggaaa aatattgcta accctatcgc cacaatttgg gccggtgcga
     8821 tgatgctcga ttttctcggc aatggcgatg agcgtttcca gcaagcgcat aacggtattc
     8881 tggcagcgat tgaagaagtg attgctcacg ggccgaaaac accggatatg aaaggcagtg
     8941 ccaccacgcc acaggttgcc gacgcgattt gcaaaattat tttgcgttaa ggtcaaacca
     9001 gtttatttga accgcgtcac tgacgcggtt tttttattcg ttctttgcag ttaataacct
     9061 gcgtcatttc accttttatt gtttccattt cgtgttttat ggctttccgt attcttaatt
     9121 gtttaattta tgtaacatgc aaataatcaa tttgttaaac acaacataca actcattacc
     9181 ctgcaaataa ttacaactgg caaaagccag agacgggctt ttatgcccag gagatgactc
     9241 atgatgagca atgttaagaa aaaagatgtg ccgctgataa gcatcagcct ggtggccatt
     9301 cttttcatcg cagctgcatt aagccttttc ccacaacaat cggccgacgc ggccaacaca
     9361 atatacactt ttgttacgcg tacgttaggt tccgccgtac aggtattggt tttgctggca
     9421 atgggactgg tgatttattt agccaccagt aaatacggca atattcgtct tggcgaagga
     9481 aaaccggaat acagcacgct ctcctggctg tttatgttta tttgtgccgg tttaggttct
     9541 tctacgcttt attggggggg tgctgaatgg gcctattatt atcaaacgcc tggattaaat
     9601 atcgcaccac gttcacaaca ggcactcgaa tttagcgtcc cttactcctt cttccactgg
     9661 ggcatcagca cctgggcaac ttatacgctg gcctcattaa tcatggctta tcactttcat
     9721 gtgcgtaaaa acaaaggtct gagtctttcc ggcattattg ccgcgattac cggcgttcgc
     9781 ccgcaaggcc catggggaaa actggttgat ttgatgtttc tgatcgccac tgtcggcgca
     9841 ctgactattt cccttgttgt caccgcagca acctttactc gtggactttc cgcgctgacc
     9901 ggtttacccg ataacttcac cgtgcaggca tttgtgatcc tgctttccgg cggcattttt
     9961 tgcctaagct cgtggattgg tatcaacaac ggtttgcaac gtctgagcaa aatggttggc
    10021 tggggcgcgt tcctgctgcc attactggtg ctgattgtcg gtccaaccga atttattacc
    10081 aacagcatca tcaatgccat cggcctgacc acgcaaaact tcctgcaaat gagcttattc
    10141 accgatccgc ttggcgatgg ttcatttacc cgcaactgga ccgttttcta ctggctgtgg
    10201 tggatctcat acccccctgg cgtagcaatg tttgtcaccc gcgtttcccg cggtcgtaag
    10261 attaaagaag ttatctgggg actgatcctc ggcagcaccg tcggttgctg gttcttcttc
    10321 ggcgtaatgg aaagctatgc cattcatcag tttatcaatg gcgtaatcaa cgtcccacag
    10381 gtgctggaaa cactgggcgg cgaaacagct gtacagcaag ttctgatgtc gttgccagcc
    10441 ggtaaattgt tcctcgccgc atacctgggc gtgatgatta ttttccttgc ctcgcatatg
    10501 gatgcagtgg cctacaccat ggcggcgacc agtacgcgta atctccagga aggtgacgat
    10561 cctgaccgtg ggctgcgtct tttctggtgc gtggtgatca ctctgatccc gctttccatc
    10621 ttgtttaccg gtgcttcgct ggaaacgatg aaaaccaccg tcgtgctcac agcccttccc
    10681 ttcctcgtca ttttactggt gaaagtcggc ggatttattc gctggctgaa acaggattat
    10741 gccgacattc cggctcatca agttgaacat tatctcccgc agacaccggt tgaagccctg
    10801 gaaaaaacac cagtgctccc tgcgggaacc gtattcaaag gcgacaactg agcgccatca
    10861 tcctaacgat aaaggtatcc ctatgagcaa tctgagccct gactttgtac tacccgaaaa
    10921 tttttgcgct aacccgcaag aggcgtggac cattcctgcc cgtttttata ccgatcagaa
    10981 cgcgtttgaa cacgaaaaag agaacgtctt cgccaaaagc tggatttgcg tcgctcacag
    11041 cagcgaactg gcgaatgcca atgattatgt gacgcgtgag atcattggcg aaagcatcgt
    11101 gctggtacgc ggtcgtgata aggttttgcg cgcgttctat aacgtgtgtc cgcaccgtgg
    11161 tcatcagttg ttgagcggtg aaggaaaagc aaaaaatgtg attacctgcc catatcacgc
    11221 atgggcattc aaactcgatg gcaatctggc ccatgcacgt aactgcgaaa acgtcgccaa
    11281 tttcgatagc gacaaagcgc aactggttcc ggtgcgtctg gaggaatatg ccggattcgt
    11341 cttcatcaac atggacccca acgccaccag cgtagaagat cagttacccg gtctgggcgc
    11401 gaaagtgctg gaagcctgcc cggaagtcca cgatctgaaa ctggcggccc gctttaccac
    11461 ccgcacgcct gccaactgga agaacattgt cgataactat ctcgagtgct atcactgtgg
    11521 tccggcgcat ccaggtttct ccgactccgt acaggttgat cgctactggc acaccatgca
    11581 cggtaactgg acgctgcaat acggtttcgc caaaccgtcc gaacagtcgt ttaaatttga
    11641 agagggtacg gatgcggcat tccacggttt ctggctgtgg ccgtgcacga tgctgaacgt
    11701 caccccgatc aaagggatga tgacggtcat ttatgaattc ccggtggatt ctgaaactac
    11761 cctgcaaaac tacgatattt acttcaccaa tgaagagtta accgacgagc aaaaatcgct
    11821 gattgagtgg tatcgcgatg tgttccgtcc ggaagattta cgtctggttg aaagcgtaca
    11881 gaaagggctg aaatcgcgtg gctatcgtgg tcaggggcgc atcatggccg acagtagcgg
    11941 tagtggcatt tccgaacatg gtatcgccca tttccataat ctgctggcgc aggtgtttaa
    12001 ggactaatga catcggcggc ggtattttcc gccgctgggc tgatttttga tggagtacag
    12061 caatgtcaga ctatcaaatg tttgaagtac aggtgagtca ggtggaaccg cttaccgaac
    12121 aggtgaaacg cttcacgctg gtggcaaccg atggcaaacc gctgcctgcg ttcaccggag
    12181 gaagtcacat cattgtgcag atgagcgatg gtgataacca gtacagcaat gcgtattcac
    12241 tactgagttc gccgcatgac acctcttgtt atcagattgc cgttcggctg gaggaaaact
    12301 cgcgcggcgg ttcccgcttt ttgcatcagc aggtaaaagt gggcgatcgg ttaacgattt
    12361 caacgcctaa taacctgttt gcgctaattc cctcagccag aaagcatctg tttatcgcgg
    12421 gcggtattgg tatcacccct ttcctgtcgc acatggcaga gttgcaacac agcgacgtcg
    12481 actggcagct acattactgc tcgcgaaatc cagaaagttg cgcatttcgt gatgagctag
    12541 tccagcatcc gcaggctgag aaagtccatt tgcatcattc gtcaaccgga acacgactgg
    12601 aattagcgcg attattggcg gatatcgaac ctggcacaca cgtttatacc tgtggccccg
    12661 aggcgctaaa tgaagcggta agaagtgaag ctgcgcgtct ggacatcgcc gccgatacgc
    12721 tgcactttga gcaatttgct atcgaagaca aaaccggcga tgcctttacc ctggtgcttg
    12781 cccgttccgg aaaagagttt gtggtgccgg aagagatgac tattttgcag gttattgaaa
    12841 ataataaagc cgcgaaagtg gaatgtttat gtcgtgaagg ggtatgcgga acctgcgaaa
    12901 cagcaatact ggaaggtgaa gctgaccatc gggatcagta ttttagcgat gaagagcgtg
    12961 ccagccagca aagtatgttg atctgttgtt cgcgtgcgaa gggtaaacgc ctggtgttgg
    13021 atttgtagtt tgccaaagcc ggatgtggcg ctgagcgcgc ccagtccggc ttcggaagat
    13081 tttactgcgg atattcctgc aataaattgt gtaatgcttc cgccatcagc tcaccacgcc
    13141 agccggaaat cagctccggc aaattgttct gcggtttcag tttccagtgc cagttcagca
    13201 gttggttgat ttgccgacgc gatgccagca attcggcgct gatcttatgc gtttcgctca
    13261 cgtcagtaat cagcgactta atcgctttaa acgctttacg ataacccggc atgtccatca
    13321 ggttaagcat cggctgcggt aaggcctctt ccggcaatgt ctgcgctttt tccaccagcg
    13381 ccagcagcgt tttaccgtga aagcggattt cgctaccgga taaacccagg ctgtccagtt
    13441 cgcctaaact tcccggcata taacgcgcga ccgaccacaa atgctcttca cgcacgacaa
    13501 agttcaccgc cagatcacgc tcacgagcct tgcgcagtcg ccagtcggct aacagttgca
    13561 gacaggccag ttggcgtgtg cgtaattgcc aggcattggt gatatcacgc caggcatctt
    13621 ccggcgcaac gacttcctga cgacgcattt gcatcaggcg gcattcatcc agcgccgcag
    13681 gtagccagcc ggaggcctcc gtttctacca taagcttggc ggtgatcggt aacagatacc
    13741 agacatccgc cgctgcgtat tcacactgac gttcggtcag cggtctggcc agccagtcgg
    13801 tgcgcgattc actcttgtcc agcgtaacgc cggaatactc ttccaccatg gaagcgaaac
    13861 cccatgacat cgggcgtccg cagaaggcag caaggatttg cgtgtcaatc aggggttgtg
    13921 gtaattcgcc aaagacattg aggaacactt ccagatcttc actgcctgca tggagaaatt
    13981 ttgtgatgga cggatcgcgc aggatcgctt tcagcggtga ccagtcggtg atcccgagag
    14041 gatcaattag cgccagatgc tcaccatcga aaagttgaat caaccccagc tgcgggtaat
    14101 aagtgcgcgt acgaacaaat tcagtatcct gggctatcgc cggaaagaca cggacggctt
    14161 cacacaaaga agccagcgca tcgtccgtgg taatcatttg gtaattcaaa ttgtattctc
    14221 tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc tgacgactga cttaacgctc
    14281 aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc aaaatttttc
    14341 cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact ttgtagcccg
    14401 tgagctgacg gcagcaaaag gtcaccagtg actcttcggt aagcgatgga tcttttttca
    14461 ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca gccgcgactt
    14521 cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag acgttaaaac
    14581 cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaaccct tcttcatcca
    14641 tcaccgcgat gtcgccggtg tgtaaccagc catttttgat gatctcatcg gtagcatcgg
    14701 gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca cccggttgac
    14761 ccggtggtac ttcattatcg tcgtcatcca ccagtttggc ttccgtcgac ggcaccggca
    14821 aaccgatgct accactatga taatcaatat catatgggtt aacgctgacc agcggcgcac
    14881 actcggtaag accatagcct tccagcagat actgtcctgt cagtttcacc caacgctctg
    14941 ccaccacttg ctgcactggc atcccaccgc ctgcggaaag atgcagactg gagaaatcca
    15001 gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg ccagtgatag
    15061 cggtaaacgg atatttcgct aactctttta ccaaccctgg aatatcgcgc gggttagtga
    15121 taagcaggtt ctgcccaccc agttcgataa acagcaggca gttaatggtc agggcaaaaa
    15181 tgtgatacag cggcagtgcc gttactacca gttctttacc tggatgcagc aacggcccat
    15241 aggtggcgtt gacctgttcc aggttcgcca gcatattgcg gtgagtcagc atcgcgcctt
    15301 tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc ggcaccagtt
    15361 cgggtttgac gtactgcatc cggtagccgt tatgcagtgc gctacgaaat gaaatggcat
    15421 ctggcagatg gtatttcggc accaaacgct tgatgtattt aacaacgaaa ttgactaccg
    15481 tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc tgaacggcgg
    15541 ttttatcaac cactttttcc agtgtgtgag caaagttaga cacgataaca atcgccgatg
    15601 cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg ttaacgttta
    15661 cgacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc aataaattag
    15721 gcatcatcaa cgcaacgcga tcgcctttct tcagccccaa cccttgttgc aaataagcgg
    15781 caaacgcgcg actgcgttct tccagcttgc ggaaggtcat tacctccccc atattcacaa
    15841 acgcaggctg atcggcgtag cggacgaccg actgctcaaa catatctatc agagattgat
    15901 aacggtcagg gttgatctcc gtcggaacat ccgcgggata acggttaagc caaaccttct
    15961 tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaagt gataaacatg
    16021 ttgttaacat aacattaact catcttacca gcttgataat tagacaacga aaaggttgcg
    16081 aagcgcgtca ctatttattt ttatagttgc cgtatgaacg cagaaacagc ggaccagccg
    16141 ctgtttcttt ttctttaaaa acaacttgtt actcggttac aattgtttca actcttgcag
    16201 ggccaggatt ataccaaccc catccgccgt aaccataggg ccagccgcgt ccaccataaa
    16261 accatggatc aattggctgc ggcggcataa tcacctgttg agtgatatgc cagcgtttgt
    16321 aacctgttac ctgcataacc ataaatttat atggtgcatt atttactttg ccatccactg
    16381 tgccagtgat tggcccgaca accgtcacca gttgtccacg aaaatcgacc ggatcaagaa
    16441 aaccgttcac atcggcataa atgcgcccgc gtgaaggttc tcccaaggtt ggtcttgctc
    16501 cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg ttttgtacca
    16561 caaccacttt gccgccaaag cgcgcctcct gaccaacata taaatctggc gcattcatca
    16621 cgcgtaccag atcttgctgt ggtgttggac tgctgccttt aatggcgtcc ggcacagtga
    16681 cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata acattctttt
    16741 gaaccgccat gatgcgactc ctttttttct cagggcatac tcttaagatt cattctttgc
    16801 ccggaagttt cttccatgcg acgttgttac gtaaataaac cggttcggca tgttccaccg
    16861 ccaccgtttt accctcggca aacatctgac acgcaatcgg cagcatatct tccgccgctg
    16921 gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc tttacggaga tccggccagg
    16981 cttgccagcc cgtccctacc gttacccatt cgccggaaag ctgttgcatt cgttcatgaa
    17041 cgagttcggg tttgagtacg gcttcggttt cttcaccgcg ccagataccg ttttcatcac
    17101 gctgatattc ggcccagtaa acttcgccca ttcgcgcgtc aatggctgcc agcacgcggg
    17161 ttgcgccgtt tttgcgccac gccccctgcg ccatcgtcat tagcgtagag acaccaatca
    17221 tcggtaactc cgcgccaagc gccagccctt gcgcaatacc aataccaatg cgcacgccag
    17281 taaagctacc ggggccgcgc ccgtaagcca gagcgtttat gtcagtcagg gaagttccgc
    17341 tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca cgagggcaaa
    17401 gctcaaaatg agcgttgaca ttaccgtcgt tccacagagc gacagagcag acctctgtcg
    17461 cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa aaggcgcgca
    17521 tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa cgaaccgcac
    17581 gggcaatgtc acgggtgcgt ggcgcgggcg gcagactggc gagaaacgtc gcgccgtaag
    17641 gacgcatcac cagccgattg tcacaaatta ccagcacgcc gcgatcgtcg gcgtcgcgaa
    17701 tcagtcgccc tactccctgc ttgagagtaa tgaccgcatc cggcagctgc acttcatcga
    17761 acgggtcgcc accgcgcaaa cgacaatctt ccatgcgcgc ttttaacagt agatcatccg
    17821 gcgaggtaaa cggcaatttg tcgataatta ccaatgacaa tgtatcgcca cgcacgtcca
    17881 ccccttccca gaaactactg gttgccacaa gaagcgcgtt accggcgctg acaaattgct
    17941 gcaacagttg ccctttgctg gtttccccct gcaacaaaac gggaagcgtc atggtagcgc
    18001 ggaactgctc ggccagatcg cgcatcatgg catgcgaggt acaaagcata aaacaacgac
    18061 cgttgttagc ttcgatgatc ggtcgcagca ttgccgccag ttgccgtgca caacccggtt
    18121 gattggtttg cggcaggttg cgcggcacac agagtaacgc ctggcggctg taatcaaacg
    18181 ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg aaatgatgca
    18241 gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccgggc ttttgcgcca
    18301 ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta aaatggcgcg
    18361 aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc cgcttcagcc
    18421 gtgtgcgata caacgtggcg cgctcaaatg ccgcatccag caaggcggaa cgccccagtg
    18481 acagtttcgc cacgtcataa caaagttcca gggtgtcatc gagcagtaaa aatgcccgct
    18541 gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctggc tcaccgagtt
    18601 gcagacgaaa atcctgtgca ctctgggcaa gacgatcagc gcacttttgt aactgctggg
    18661 tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg agcagttgtc
    18721 gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg tgggcttcgt
    18781 cgaagatcat gacgtccgct tcagggatca actcgccaaa tccactctct ttaaccacca
    18841 tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc gcttttttac
    18901 gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga cagttgtcgt
    18961 tggtgctggt gaccagcggc cacgcctgtg aatcttccgc cacgctgacg caggtgctga
    19021 tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg cttaagattt
    19081 gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg cagaggtagt
    19141 ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt gagactgttg
    19201 gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag ataatgactt
    19261 tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta ccggttcctg
    19321 cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc gccatctgtc
    19381 gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg tctggtgcaa
    19441 aatcgtccgt cacactaccc cctgttgatt tgaacaggga ttatgtcagg atgagggcgc
    19501 tttcgccagt tgaagtggtg acggcaacct cacattgtgg cagtctttgc agcacgaaaa
    19561 tggaagtata atgaggacaa aatgatgact atcgttcgta tcgatgctga agcccgctgg
    19621 tctgatgtag taatccacaa caacacgctc tactacactg gtgtaccgga aaacctcgac
    19681 gccgatgcct ttgagcaaac cgccaacacg ctggcacaga ttgacgccgt gctggaaaaa
    19741 cagggcagca ataaatcgag cattctggat gccaccattt tcctggccga taaaaacgac
    19801 ttcgcggcga tgaataaagc gtgggatgcc tgggttgtcg cgggtcatgc gccagtgcgc
    19861 tgcacggtac aagcgggttt gatgaacccg aagtataaag ttgaaattaa gattgtggct
    19921 gcggtgtaag ctttatcgaa gcaaaataag tcagacgata atttatcgat aatactggtc
    19981 ggttttacat aaatcgacca gaga
//
LOCUS       DAZOYL010000047        19818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZOYL010000047 DAZOYL010000000
VERSION     DAZOYL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.047"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHUOY_001242"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHUOY_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR0682071.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHUOY_001243"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHUOY_001243"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR0682072.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHUOY_001244"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHUOY_001244"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR0682073.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHUOY_001245"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHUOY_001245"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR0682074.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHUOY_001246"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHUOY_001246"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR0682075.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="ACHUOY_001247"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="ACHUOY_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR0682076.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11312
                     /gene="ftsK"
                     /locus_tag="ACHUOY_001248"
     CDS             7245..11312
                     /gene="ftsK"
                     /locus_tag="ACHUOY_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR0682077.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11467..12078
                     /gene="lolA"
                     /locus_tag="ACHUOY_001249"
     CDS             11467..12078
                     /gene="lolA"
                     /locus_tag="ACHUOY_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR0682078.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12089..13432
                     /gene="rarA"
                     /locus_tag="ACHUOY_001250"
                     /gene_synonym="mgsA"
     CDS             12089..13432
                     /gene="rarA"
                     /locus_tag="ACHUOY_001250"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR0682079.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13523..14815
                     /gene="serS"
                     /locus_tag="ACHUOY_001251"
     CDS             13523..14815
                     /gene="serS"
                     /locus_tag="ACHUOY_001251"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR0682080.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15054..17498
                     /gene="dmsA"
                     /locus_tag="ACHUOY_001252"
     CDS             15054..17498
                     /gene="dmsA"
                     /locus_tag="ACHUOY_001252"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR0682081.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17509..18126
                     /gene="dmsB"
                     /locus_tag="ACHUOY_001253"
     CDS             17509..18126
                     /gene="dmsB"
                     /locus_tag="ACHUOY_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR0682082.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18128..18991
                     /gene="dmsC"
                     /locus_tag="ACHUOY_001254"
     CDS             18128..18991
                     /gene="dmsC"
                     /locus_tag="ACHUOY_001254"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR0682083.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19027..19653)
                     /gene="ycaC"
                     /locus_tag="ACHUOY_001255"
     CDS             complement(19027..19653)
                     /gene="ycaC"
                     /locus_tag="ACHUOY_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR0682084.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
BASE COUNT         4967 a         5022 c         5263 g         4566 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cataaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc atttttgtgg tgaaaacaag catcgtggca
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataattc agataatccc
      841 gacggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgactg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcatgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca accttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattcaggc caaagcaccg
     1261 cgcgaggatc gggcgaccac cagaggatgg gggcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gccccccaga gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgctta aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcgtcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccaa gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgac
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcgattgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcacccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcatgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg attttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcaacag tgaactttta cctgaaccgc tgcgaccaac
     3841 caacacggca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc accggatcgg tcgatgctaa
     3961 ttccacctcg ccacgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaatgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 tgaggggaag attgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccgcc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt caggggcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccttg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
     7921 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc tgacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc ggcacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagccacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccacagcagc
     8461 cgtattatgc acctgcagct gaacaacctg tgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattacgccc ctgcgccaga acaaccggtg gcaggtaacg
     8581 cctggcaagc cgaagagcag caatccactt ttgctccaca gtctacatac cagactgagc
     8641 aaacttatca gcaaccagcc gctcaggagc cgttgtacca acagccgcaa cccgttgaac
     8701 agcagcctgt tgtggagcct gaacccgttg tagaagagac aaaacccgcg cgtccgccac
     8761 tttactattt tgaagaagtg gaagagaagc gagcccgtga acgtgaacaa cttgcggcct
     8821 ggtatcaacc gattccagaa ccggttaaag aaccagaacc gatcaaatct tcgctgaaag
     8881 caccttctgt tgcagcagta cctccagtag aaaccgctgc cgctgtttct ccgctggcat
     8941 ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc aaccgttgcc gcgccagtct
     9001 tcagtctggc aaatagcggt ggaccgcgtc cgcaggtcaa agaggggatt ggcccgcagt
     9061 tgccacgacc gaaacgtatc cgcgtgccaa ctcgccgtga attggcgtct tacggtatta
     9121 agctgccctc acagcgtgcg gcggaagaaa aagcccgtga agcccagcgc aatcagtacg
     9181 attctggcga tcagtacaac gatgatgaaa tcgatgcgat gcagcaggat gaactggcac
     9241 gtcagttcgc ccagacacag cagcaacgct atggcgaaca gtatcaacat gatgtgcccg
     9301 taaacgcaga agatgcagat gctgcggcag aggctgaact ggctcgtcag tttgcgcaaa
     9361 ctcaacaaca acgttattcc ggcgaacaac cggctggggc gaatccgttc tcgctggatg
     9421 attttgaatt ttcgccaatg aaagcgttgc tggatgatgg tccacacgaa ccgttgttta
     9481 cgccaattgt tgaacctgta cagcagccgc aacaaccggt tgcaccgcag cagcagtatc
     9541 agcagccaca acagccggtt gcgccacagc agcaatatca gcagccgcaa cagccggttg
     9601 cgccgcagcc acaatatcag cagccgcaac aaccggttgc gccacagcag caatatcagc
     9661 agccgcaaca accagttgcg ccgcagccgc agtatcaaca gccgcaacaa ccggttgcgc
     9721 cgcagcagca gtatcagcag ccacaacagc cagttgcgcc acaaccgcag gataccctgc
     9781 ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt gcataaaccg acgacgccgc
     9841 tgccttctct ggatttgctg acaccgccgc cgagcgaagt ggagccggta gatacctttg
     9901 cgcttgaaca aatggctcgc ctggtggaag cgcgtctggc tgatttccgt attaaagccg
     9961 atgttgtcaa ttactctccg gggccggtta tcactcgctt tgaattgaac ctggcaccgg
    10021 gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct tgcccgttca ctttcgacgg
    10081 tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtaggtctg gagttaccga
    10141 ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga taacgccaaa ttccgcgata
    10201 atccgtcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg gtggttgccg
    10261 atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt aaatctgtcg
    10321 gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
    10381 tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcattccg catctgttaa
    10441 cggaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
    10501 tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
    10561 aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac tggaagccgg
    10621 gtgacagtat ggatgcccag catccggtgc tgaaaaaaga accatacatt gtggtgttgg
    10681 ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag ctgatagcac
    10741 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaact cagcgtccat
    10801 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
    10861 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg
    10921 ggatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
    10981 cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
    11041 cacagtatgt tgatggcatc acttccgaca gtgaaagcga aggtggcgcg ggcggtttcg
    11101 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
    11161 gcaaagcgtc aatttctggc gtacagcgtc agttccgcat tggttataac cgtgcagcgc
    11221 gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac aacggtaatc
    11281 gtgaagtgct ggctccaccg ccgtttgact aattaatgca ttgccggata aggcgcggta
    11341 gcgtcgcatc cggcactcta tcaactgaaa attcagtatt ttcttctttc ctcaagctga
    11401 ttattagcct ggaatagaga gtagagggaa ctcccgatcg ggagtgacgt aatttgagga
    11461 ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt agcaagcagc
    11521 gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag cagcttccac
    11581 gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga aggtcagggc
    11641 gatctgtggg tgaaacgtcc aaacttattc aactggcata tgacacaacc tgatgaaagc
    11701 attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga gcaagctacg
    11761 gcaacttggc tgaaagatgc caccggtaat acgccgttta tgctgattgc ccgcaaccag
    11821 tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt cctgacgccg
    11881 aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga tggcacaatc
    11941 catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcaact gaaatcccag
    12001 caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg cgtcacggta
    12061 gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt tttcggataa
    12121 tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt atatcggcca
    12181 gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct atcgaagccg ggcatctgca
    12241 ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg aagtgattgc
    12301 ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggcg tgaaagagat
    12361 tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca ggtcgccgca ctattctttt
    12421 tgttgacgaa gtgcaccgat tcaacaaaag ccagcaggat gcatttctgc cacatattga
    12481 agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc
    12541 ggcactgctt tcccgtgccc gtgtctatct gttgaaatcc ctgggtacag aggatattga
    12601 gcaagtacta actcaggcga tggaagacaa gacccgtggt tatggtggtc aggatattgt
    12661 tctgccagat gaaacacgac gcgccattgc tgaactggtg aatggcgacg cgcgccgggc
    12721 gttaaatacg ctggaaatga tggcggatat ggccgaagtc aatgatagcg gtaagcgggt
    12781 cctgaagcct gaattattga ccgaaatcgc cggtgagcga agcgcccgct ttgataacaa
    12841 aggcgatcgc ttttacgatc tcatttccgc actgcataag tcggtacgtg gtagcgcacc
    12901 cgatgcggcg ctctactggt atgcgcgaat tattaccgct ggtggcgatc cgttatatgt
    12961 cgcgcgtcgt tgcctggcaa ttgcttccga agatgtcggc aatgccgacc cacgggcgat
    13021 gcaggtggca attgcggcct gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg
    13081 cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg ctgtctacac
    13141 tgcgtttaaa gctgcgctgg cggatgctcg cgaacgcccg gattatgacg tgccggttca
    13201 tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg aatatcgtta
    13261 cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc cggaaatagc
    13321 acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg gcgaaaagct
    13381 cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt aatgttatcg
    13441 ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat ctcccattta
    13501 attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg agccagacgc
    13561 agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc tgggcgctct
    13621 tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg agcgtaactc
    13681 ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc ctttacgtct
    13741 ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg atgctttaca
    13801 ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg cctgcagatg aagtgccggt
    13861 aggtaaagac gaaaatgaca acgttgaagt cagccgctgg ggtaccccgc gtgagtttga
    13921 ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac tctggcctcg actttgcagc
    13981 tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa gggcagattg ctcgcatgca
    14041 ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct acagtgagaa
    14101 ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc tgccgaaatt
    14161 tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca gtaactatgc
    14221 gctgatccca acggcagaag ttccgctgac taacctggta cgcggtgaaa tcatcgatga
    14281 agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg aagccggttc
    14341 atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag ttgaaatggt
    14401 gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg gtcatgcaga
    14461 aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgca ctggcgacat
    14521 gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac agaacaccta
    14581 ccgtgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta tgcaggcacg
    14641 ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg gttctggtct
    14701 ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat cagcaggctg atggtcgtat
    14761 tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg gctaataccc
    14821 aatttttctg aatctaaaaa gcgcctgcgg gcgctttttt tgtctccctt tgataccgaa
    14881 caataattac tcttcactta cacgtaatac tactttcgag tgaaaatcta cctatctctt
    14941 tgattttcaa attattcgat gtatacaagc ctatatagcg aactgctata gaaataatta
    15001 cacaatacgg tttgttactg gaatcaatcg tgagcaagct tgagtgagcc attatgaaaa
    15061 cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg gtaaaaacga
    15121 cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt agtcggattg
    15181 cgcacgctgt cgatagcgcc attccaacaa aatcagacga aaaggttatc tggagcgcct
    15241 gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca
    15301 aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac caggttcgcg
    15361 cctgcctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc
    15421 cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc tgggaagaag
    15481 cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc aacgagtcta
    15541 tctatctgaa ctatggcacc ggtacgctgg gcggcaccat gacccgctcc tggccgccgg
    15601 gaaataccct ggtcgcgcgg ctgatgaact gctgcggcgg ctatctgaac cattacggcg
    15661 actactcctc cgcgcaaatt gcggaaggtt tgaactatac ctacggcggc tgggcagatg
    15721 gcaacagccc gtcggatatc gaaaacagta agctggtagt gctgtttggt aataaccctg
    15781 gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggca cgccagaaat
    15841 ctaatgcccg catgatcatc atcgatccgc gctataccga caccggtgcc gggcgcgaag
    15901 atgagtggat ccctattcgt ccgggaacag atgccgcact ggttaacggt ctggcgtacg
    15961 tcatgatcac tgaaaacctg gtggatcagg cattcctcga taaatattgc gttggctacg
    16021 atgagaaaac cctgccagcc agtgcgccga aaaatggcca ctataaagct tatattctgg
    16081 gtgaagggcc agatggcgtg gctaaaacac cggaatgggc ctcacaaatc accggtgttc
    16141 cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg gcgtttatca
    16201 gccagggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt gctatctcga
    16261 tgctggcgat tctgaccggt aacgttggta ttaatggagg gaacagcggc gcgcgtgaag
    16321 gttcatacag cttaccgttt gtccgtatgc cgaccttgga aaacccgatc cagaccagca
    16381 tttcgatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg
    16441 atggtgtacg cgggaaagat aagctggatg tgccgatcaa aatgatctgg aactatgccg
    16501 gtaactgcct ggttaaccag cattctgaaa tcaaccgtac ccatgaaatc cttcaggatg
    16561 ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatcg gcgaaatatg
    16621 ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgcg ctggatgcat
    16681 cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg cgctttgaat
    16741 gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt gagcaacagt
    16801 ttactgaagg ccgtacccag gaagagtgga tgcggcatct gtatgcccag tcgcgggaag
    16861 cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg
    16921 acccacaagg gcatcacgtt gcttataaag ccttccgtga agatccgcag gcaaacccac
    16981 tgactacgcc atcgggcaaa attgagattt attcgcaggc gctggctgac attgccgcta
    17041 cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctacacg ccgggctttg
    17101 aaagttatca ggatccgctg aacaaacagt atccgctgca gcttacgggt ttccactata
    17161 aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcagct tgccgtcagg
    17221 aaatgtggat caacccgctt gatgcgcaaa aacgcggtat caacaacggc gataaagtca
    17281 ggatctttaa cgatcgtggt gaagttcata ttgaggcgaa agtgacgcca cgaatgatgc
    17341 caggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatgcaaaa cgtgtcgata
    17401 agggtggttg tattaacgta ctgaccactc aacgtccgtc tcctctcgct aaggggaatc
    17461 cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat gacaacccag
    17521 tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga gctggcttgt
    17581 aaagactaca aagatttgac gccagaagtc agcttccgcc gcatttatga atatgctggc
    17641 ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta ctatctgtcg
    17701 atttcgtgca accactgcga agatccggct tgtactaaag tctgcccgag cggtgcgatg
    17761 cataaacgtg aagatggttt tgttgtggtc gatgaagatg tgtgcattgg ctgccgctac
    17821 tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg ccatatgacc
    17881 aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct
    17941 tgtccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa acatggcgat
    18001 ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat tgtgatcaaa
    18061 cccaatgcca atagccgccc gaccggggat accactggct atctggcaaa cccgaaggag
    18121 gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg tcttcgggca
    18181 atgtgtggcg ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc
    18241 agaagcccag cagcgtgtta tcgcctgcat gtttggctta tgggtgctga tgggcattgg
    18301 ctttatcgct tctatgctcc atcttggttc accgatgcgc gcttttaact cgctcaaccg
    18361 ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt ttgccgtagg
    18421 cggcatcggc tggttgctgg caatgctgaa aaagctgtca ccggcattgc gtacgctttg
    18481 gctggtggtg acgatggttc ttggcgtcgt ctttgtctgg atgatggtgc gtgtgtataa
    18541 cagcattgat accgttccga cctggtacag catctggacg ccgatgggct tcttcctgac
    18601 gatgttcatg ggcggcccgt tactggggta cctgttgctg agtctggctg gcgtcgatag
    18661 ctgggcgatg cgtctgctgc cagccatttc cgtactggca ctggtggtga gtggcgtggt
    18721 gtcagtgatg cagggcgcag agctggcgac tattcatagt tctgtgcagc aggccgcagc
    18781 gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct
    18841 gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc tactatccgt
    18901 ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct atggtttgca
    18961 tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt cggccccggt
    19021 tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac gataatcagg
    19081 aatatggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt cacggtgcag
    19141 ctcgcaagcc acgccgaacc atgtcatcaa ttgcgcacct gcttgcgaca tgcgatccca
    19201 tgccgaatgc cgggtaattt cattaaaggt ccctgacgcg tcagtcacaa caaacacatc
    19261 aaacccttct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg ttaccacacc
    19321 ggcaataatt aactgttttt tacctgtcgc tttgactgct tttacaaaat cttcgttatc
    19381 ccaggcgtta atattgcccg ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag
    19441 ttctggaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa tagttggtaa
    19501 gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact tatcgggttc
    19561 gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa cagcagcatc
    19621 atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag agtcagtggt
    19681 acgcctgaaa tcggtcagac gtgatgcatt gctctgaaag catagacggg aaatatgagt
    19741 ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat
    19801 aaaaacaatc agatgaga
//
LOCUS       DAZOYL010000048        19708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZOYL010000048 DAZOYL010000000
VERSION     DAZOYL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.048"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHUOY_001256"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHUOY_001256"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0682085.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUOY_001257"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUOY_001257"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR0682086.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUOY_001258"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUOY_001258"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR0682087.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUOY_001259"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUOY_001259"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR0682088.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUOY_001260"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUOY_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR0682089.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUOY_001261"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUOY_001261"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR0682090.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUOY_001262"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUOY_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR0682091.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUOY_001263"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUOY_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR0682092.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHUOY_001264"
     CDS             6537..6773
                     /locus_tag="ACHUOY_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR0682093.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHUOY_001265"
     CDS             6776..7633
                     /locus_tag="ACHUOY_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0682094.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHUOY_001266"
     CDS             7654..9645
                     /locus_tag="ACHUOY_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0682095.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUOY_001267"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUOY_001267"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR0682096.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHUOY_001268"
     CDS             complement(10248..11144)
                     /locus_tag="ACHUOY_001268"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR0682097.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHUOY_001269"
     CDS             complement(11193..11432)
                     /locus_tag="ACHUOY_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR0682098.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUOY_001270"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUOY_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR0682099.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUOY_001271"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUOY_001271"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR0682100.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUOY_001272"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUOY_001272"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR0682101.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUOY_001273"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUOY_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR0682102.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHUOY_001274"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHUOY_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUOY_001275"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUOY_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR0682103.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
BASE COUNT         4541 a         5223 c         5116 g         4828 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttc
//
LOCUS       DAZOYL010000049        19449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZOYL010000049 DAZOYL010000000
VERSION     DAZOYL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.049"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            132..1061
                     /locus_tag="ACHUOY_001276"
     CDS             132..1061
                     /locus_tag="ACHUOY_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR0682104.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            1157..3493
                     /gene="arcB"
                     /locus_tag="ACHUOY_001277"
     CDS             1157..3493
                     /gene="arcB"
                     /locus_tag="ACHUOY_001277"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR0682105.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            3723..4376
                     /gene="elbB"
                     /locus_tag="ACHUOY_001278"
     CDS             3723..4376
                     /gene="elbB"
                     /locus_tag="ACHUOY_001278"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR0682106.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            4373..5101
                     /gene="mtgA"
                     /locus_tag="ACHUOY_001279"
     CDS             4373..5101
                     /gene="mtgA"
                     /locus_tag="ACHUOY_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR0682107.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(5098..5730)
                     /gene="yrbL"
                     /locus_tag="ACHUOY_001280"
     CDS             complement(5098..5730)
                     /gene="yrbL"
                     /locus_tag="ACHUOY_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR0682108.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(5944..6216)
                     /gene="npr"
                     /locus_tag="ACHUOY_001281"
     CDS             complement(5944..6216)
                     /gene="npr"
                     /locus_tag="ACHUOY_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR0682109.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(6213..7067)
                     /gene="rapZ"
                     /locus_tag="ACHUOY_001282"
     CDS             complement(6213..7067)
                     /gene="rapZ"
                     /locus_tag="ACHUOY_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR0682110.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(7113..7604)
                     /gene="ptsN"
                     /locus_tag="ACHUOY_001283"
     CDS             complement(7113..7604)
                     /gene="ptsN"
                     /locus_tag="ACHUOY_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR0682111.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(7722..8009)
                     /gene="hpf"
                     /locus_tag="ACHUOY_001284"
     CDS             complement(7722..8009)
                     /gene="hpf"
                     /locus_tag="ACHUOY_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR0682112.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(8032..9465)
                     /gene="rpoN"
                     /locus_tag="ACHUOY_001285"
     CDS             complement(8032..9465)
                     /gene="rpoN"
                     /locus_tag="ACHUOY_001285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR0682113.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(9513..10238)
                     /gene="lptB"
                     /locus_tag="ACHUOY_001286"
     CDS             complement(9513..10238)
                     /gene="lptB"
                     /locus_tag="ACHUOY_001286"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR0682114.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(10245..10802)
                     /gene="lptA"
                     /locus_tag="ACHUOY_001287"
     CDS             complement(10245..10802)
                     /gene="lptA"
                     /locus_tag="ACHUOY_001287"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR0682115.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(10771..11346)
                     /gene="lptC"
                     /locus_tag="ACHUOY_001288"
     CDS             complement(10771..11346)
                     /gene="lptC"
                     /locus_tag="ACHUOY_001288"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR0682116.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(11343..11909)
                     /gene="kdsC"
                     /locus_tag="ACHUOY_001289"
     CDS             complement(11343..11909)
                     /gene="kdsC"
                     /locus_tag="ACHUOY_001289"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR0682117.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(11930..12916)
                     /gene="kdsD"
                     /locus_tag="ACHUOY_001290"
     CDS             complement(11930..12916)
                     /gene="kdsD"
                     /locus_tag="ACHUOY_001290"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR0682118.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(12930..13907)
                     /locus_tag="ACHUOY_001291"
     CDS             complement(12930..13907)
                     /locus_tag="ACHUOY_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR0682119.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            14117..14926
                     /gene="mlaF"
                     /locus_tag="ACHUOY_001292"
     CDS             14117..14926
                     /gene="mlaF"
                     /locus_tag="ACHUOY_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR0682120.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            14934..15716
                     /gene="mlaE"
                     /locus_tag="ACHUOY_001293"
     CDS             14934..15716
                     /gene="mlaE"
                     /locus_tag="ACHUOY_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR0682121.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLANCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            15721..16272
                     /gene="mlaD"
                     /locus_tag="ACHUOY_001294"
     CDS             15721..16272
                     /gene="mlaD"
                     /locus_tag="ACHUOY_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR0682122.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            16291..16926
                     /gene="mlaC"
                     /locus_tag="ACHUOY_001295"
     CDS             16291..16926
                     /gene="mlaC"
                     /locus_tag="ACHUOY_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR0682123.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            16926..17219
                     /gene="mlaB"
                     /locus_tag="ACHUOY_001296"
     CDS             16926..17219
                     /gene="mlaB"
                     /locus_tag="ACHUOY_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR0682124.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            17379..17633
                     /gene="ibaG"
                     /locus_tag="ACHUOY_001297"
     CDS             17379..17633
                     /gene="ibaG"
                     /locus_tag="ACHUOY_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR0682125.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            17688..18947
                     /gene="murA"
                     /locus_tag="ACHUOY_001298"
     CDS             17688..18947
                     /gene="murA"
                     /locus_tag="ACHUOY_001298"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR0682126.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18995..19273)
                     /gene="sfsB"
                     /locus_tag="ACHUOY_001299"
     CDS             complement(18995..19273)
                     /gene="sfsB"
                     /locus_tag="ACHUOY_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR0682127.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
BASE COUNT         4599 a         4783 c         5218 g         4849 t
ORIGIN      
        1 atgaaatttc ggtaattttg attgattatg cagttgataa aagcgggccg ggacgattga
       61 tccaggtcat cgccgaattg agctaaaaat ggcaggcttt ggcctctttt caaggcgcgg
      121 tctatcagat tatgcagtta cagaaattag tcaatatgtt tggtggtgat ctcacccgtc
      181 gttatgggca aaaggtgcat aagctgacgc tgcacggcgg tttcagttgc ccgaatcgcg
      241 acggcaccat cgggcgcggc ggctgcacct tctgtaatgt tgcctcgttt gccgacgaag
      301 cgcagcagca tcgttccatt gccgaacagc tggcgcatca ggcgaattta attaatcgcg
      361 cgaaacgtta cctcgcctat ttccaggcgt ataccagcac ctttgccgaa gttcaggtgc
      421 tgcgttcgat gtatcaacag gcggtgagcc aggccaatat tgtcggtttg tgtgtcggca
      481 cgcgcccgga ctgcgtgccg gatgcggtgc tggatctgct ttgcgaatat aaagaccagg
      541 gctacgaagt gtggctggag ctggggctgc aaaccgtgca cgacaaaacg ctacatcgca
      601 ttaatcgcgg tcatgatttc gcctgttatc agcgcacgac gcagctggca cgccagcgcg
      661 ggctgaaggt gtgctcgcat ttaattgtcg gtctgccagg cgaagggctg gctgaatgtc
      721 tgcaaacgct ggagcgggta gtggaaaccg gtgttgacgg tataaagcta catccgttgc
      781 atattgtgaa aggcagcatt atggcgaaag cctgggaagc ggggcgtttg aacggtattg
      841 aactggagga ttacacgctc actgccggag aaatgattcg ccatacgcca ccggaagtga
      901 tctaccaccg catttccgcc agcgcccgtc gcccgacgct gcttgctcct ctgtggtgcg
      961 aaaatcgatg gacgggaatg gtcgagctgg atcgctatct gaatgaacat ggcgtacagg
     1021 gatcggcgct gggacgtccg tggctacctc caacggagta ggtcgttgag gggaattccg
     1081 catttctcac acaatttata acgtaactgt cagaattggg tattattggg gcaggttgtc
     1141 gtgaaggaat tccctaatga agcaaattcg tctgctggcg cagtattatg ttgacctgat
     1201 gatgaagtta ggtctggtgc gcttctcaat gttgctggcg ctggccctcg tcgttcttgc
     1261 cattgtggta caaatggcgg taaccatggt gctgcatggt caggtcgaaa gcattgatgt
     1321 tattcgttct atcttctttg gtttgttgat tacgccgtgg gcggtctact ttctatcggt
     1381 ggtcgtcgag caactggagg agtcacgaca acgtctgtca cggctggtgc aaaaactgga
     1441 ggagatgcgc gagcgcgatt tgagcctcaa cgttcagtta aaagataata ttgcccagct
     1501 aaatcaggaa atcgccgttc gtgaaaaagc ggaagcagaa ctgcaggaaa ccttcggcca
     1561 actgaaaatt gaaatcaaag agcgcgaaga gacacaaatt cagctcgagc agcaatcctc
     1621 attcttacgt tccttccttg atgcttcacc cgacctggtt ttttatcgta acgaagataa
     1681 agagttttcc ggctgtaacc gcgcgatgga gctgctgacc ggaaaaagcg aaaaacaact
     1741 ggttcacctg aaacctgttg atgtttactc accggaagcc gccgcaaaag tcattgaaac
     1801 cgatgaaaaa gtgttccgtc ataatgtgtc actgacctat gaacagtggc tggattaccc
     1861 ggacgggcgc aaagcctgct ttgaaatccg taaagtgccg tactacgacc gcgtgggtaa
     1921 acgtcacggt ttgatgggct ttggtcgaga cattaccgag cgtaagcggt atcaggatgc
     1981 gcttgaacgg gccagccgcg acaaaacgac gtttatctcc accatcagtc acgaattgcg
     2041 tacaccgctg aacggtatcg tcggtctgag ccgcattctg ctggataccg aactcaccgc
     2101 cgagcaggaa aaatatctca agaccatcca tgtttcggcc gtcacgctgg ggaatatctt
     2161 taacgatatt atcgacatgg ataagatgga acggcgcaag gtccagcttg ataatcaacc
     2221 ggttgatttc accagcttcc ttgccgatct ggaaaatctc tccgcattgc aggcgcaaca
     2281 aaaaggattg cgctttaacc tggagccgac gctgccatta ccgcatcagg tcattaccga
     2341 cgggacgcgt ttacggcaga tcctgtggaa cctcatcagt aacgccgtca aattcaccca
     2401 gcaaggccag gttaccgtgc gcgtgcgcta cgatgaaggc gatatgctgc attttgaagt
     2461 ggaagactct ggtatcggca ttccgcagga tgagctggat aaaattttcg ccatgtatta
     2521 ccaggtgaaa gacagtcatg gcggtaaacc tgccaccggc accggtattg gtctggccgt
     2581 ttctcgtcgt ctggcgaaaa atatgggcgg cgatattacg gttaccagcg aacagggcaa
     2641 aggttcaacc tttacgttga cgatccacgc accgtcggta gcagaagagg tcgatgatgc
     2701 gtttgatgaa gacgatatgc ctttaccggc gctgaatgtg ctgctggtgg aagacattga
     2761 actgaacgtg attgttgcgc gttctgtgct ggaaaaatta ggtaacagcg ttgatgtcgc
     2821 catgaccggc aaggcggcgc tagagatgtt taaaccgggc gaatacgacc tggtgttact
     2881 ggatattcag ttgccagata tgaccgggct ggatatctct cgtgaactga cgaagcgtta
     2941 tccgcgagag gatttaccgc cgctggtggc cttaaccgct aacgtgctga aagacaaaca
     3001 agagtacctc aatgctggaa tggatgatgt gctgagtaag ccgctttctg ttccggcgct
     3061 aaccgcgatg atcaagaaat tctgggatac ccaggatgat gaggagagta cggtgacgac
     3121 agaagagaac agtaaatcag aagcattgct cgatattccc atgctggaac agtatctcga
     3181 acttgtagga ccgaagctga tcaccgacgg gttagcggtg tttgagaaga tgatgccggg
     3241 ctatgtcagc gtgctggagt cgaatctgac ggcgcaggat aaaaaaggca ttgttgagga
     3301 aggacataaa attaaaggtg cggcggggtc agtggggtta cgccatctgc aacagctggg
     3361 tcagcaaatt cagtctcctg accttcctgc ctgggaagat aacgtcggtg aatggattga
     3421 agagatgaaa gaagagtggc gtcacgacgt agaagtgctg aaagcgtggg tggcaaaagc
     3481 cactaaaaaa tgaccccggc tagaccgggg tgcgcgaata ctgcgccaac accagggaaa
     3541 tcttggctgc gccgtaaatt attatgatga gttacaaggg cacagcactg tttttcaggc
     3601 cgcacgcagt taagatagca aaacttaaat gatttgttac atgaatcagt taaatgtgtg
     3661 atgcgtacca tttttattaa aaaaattaat gtgtttcagt caattgcgaa aggatgatca
     3721 caatgaagaa aattggcgta attctgagcg gatgcggcgt ctatgacggt tctgaaattc
     3781 atgaagcggt gttgacgttg ttagctattt cacgcagcgg tgcgcaggcg gtctgctttg
     3841 caccggataa gcagcaggtt gatgttatca accatttaac tggcgaagcg atgacggaaa
     3901 cgcgcaacgt gctgattgaa gcggcacgaa taacgcgcgg tgaaatccgt cctctggccc
     3961 aggccgatgc cgctgaactg gatgcgttga ttgtgccggg ggggtttggc gcggcgaaga
     4021 atttaagcaa ttttgccact cttggtagcg aatgcaccgt tgaccgtgaa ttaaaggcgc
     4081 tggcacaagc gatgcatcag gccggaaaac cgcttggttt tatgtgtatt gccccggcga
     4141 tgctgccgaa aattttcgat ttcccgctgc gtttgaccat cggtactgat atcgataccg
     4201 cagaagtgct ggaagagatg ggcgcggagc atgtgccctg tcctgtcgat gatatcgtgg
     4261 ttgatgaaga caataagatt gtcaccaccc cagcatatat gctggcgcag aacattgcag
     4321 aagcggcgag cggcattgat aagctggttt cccgcgtgct ggttctggct gaatgagtaa
     4381 aagccgctta acggtgttta gtttcgttcg ccgttttctt ttgctgttaa tggttgtcct
     4441 cgccgttttc tggggcgggg gcatcgcgtt gtttagtgtt gcgcctgttc ccttctcagc
     4501 ggtaatggtc gagcgacagg tcagcgcctg gctgcatggc aattttcgtt acgtggcaca
     4561 ttctgactgg gtcagtatgg atcaaatctc gccgtggatg ggactggcgg tgattgccgc
     4621 agaagatcag aaattttctg agcactgggg ctttgatgtc gcttccattg agaaagccct
     4681 ggcgcacaac gagcgcaatg aaaaccgtat tcgcggtgct tcaacgattt ctcagcagac
     4741 ggccaaaaat ctctttttat gggatgggcg tagctgggta cgaaaagggc tggaagctgg
     4801 attaacgctg gggatagaaa cagtctggag caaaaagcgt atcctgacgg tttacctgaa
     4861 tatcgccgaa tttggcgacg gtgtgtttgg cgtcgaagct gcggcacaac gttatttcca
     4921 caaacccgcg agcaaactta cccggtcgga agctgcatta ctggcagctg tattacctaa
     4981 tccacttcgt ttcaaagtct cctcgccatc gggctacgtg cgtagccgtc aggcgtggat
     5041 tttacggcag atgtatcagt taggcggtga gccgtttatg caacaacacc agctggatta
     5101 agcttcgcgc gaagtgaggg ccagcgtttt gctctcacga ggttgcagat ctttctgcag
     5161 ggcaatagcc agcgccggtt gggcgataaa tcgtttccat aaccgctctt gcttacgcag
     5221 acaacaccac ttcgaccagg tcgccagtgg aatcaatgtg ctttcaccaa tgttgtcgca
     5281 gacgaccggg ataacctctg actcactgat gcggtgacag aggatattct gcggcttcag
     5341 cgacatcgtc acgatacggt tatcctgcaa ataacgcttc agctgtttca gtagctggcg
     5401 cagttgtgca atgtcttctt cgtaacggca ttgttcagca aattcggtca gcgtaatgga
     5461 gagtttgccg tcaaaatctg caatcacatc gtagacataa ccggttccgc aatccgtctc
     5521 aacggtaccg tgatagcgcg gtattccact ccagtctttt aaccggcgac caagatgcgc
     5581 gtagtacttt aactcacggc ggatctcttt atcgccacca tcgccacgat ggtagacaat
     5641 cttgatacag cgttgggcat cttccggatg cgcataacat ttccgatgtc ttccggtgcc
     5701 caggggactt tgttcagata agcgaatcat gccatctcct ggaaatattt tctgacgatc
     5761 tccagtatgg tcgattaact taaacaaaac ctaaacaatg cctcttaagg ttttcttaag
     5821 gttcttctga aagtgaaaag cgcacaaaat gtacaaatga cgttgattga gaacgatttc
     5881 agtacgtgat tttcttattg tcggggggag tttgaaggga gttgtatgtc aaagtgatga
     5941 agattaatct tcatcaaaac cagagttaaa gagggcgata acggcggcca gtgcttcctc
     6001 ttcctgtggg ccggtcgctt caacttcaat ctgccgtcct ttggcagaat ccaacatcag
     6061 cagcgcaata acgctgttgg cttcggcctc ggtgccttcg tcattacgta acagcacttc
     6121 agcgtcaaaa ccctgcatta attcaaacag cttcattgca ggccgggcat gcatgcccag
     6181 cttgtttgtg atttcaacag tttgcttgac ggtcatggtt tacgtttttc cagcgtacga
     6241 tggcgtgact ggacgttttt accgcgcgag cggaagtagt ctgccagttg ctctgcaata
     6301 tacaccgaac ggtgcttccc gccggtacaa ccaatggcga ccgtcaagta gctacggttg
     6361 ttggtttcca gcataggtaa ccatagctca agatagcttc gcgtctggta gataaaattg
     6421 tgtacttctg tgtggcggtc gaggaacgcg gcgacaggtt tatcaagacc tgtcattgga
     6481 cgcagtttcg gatcccagtg cgggttcggc aagaagcgca cgtcaaagac gtaatctgca
     6541 tcgataggga taccgtgttt gaagccgaaa gactcaaaga ccatggtcag ttcgcgttca
     6601 cgtttaccca gcagacgggt acgcagcatt tcagccagct cgtgaacgga catttctgag
     6661 gtatcgacaa tcagatccgc tcgcgaacgc agaggctcca gcaaatcgct ttctttgtcg
     6721 atagcacttt ccagcgacag gtttttgctg gaaagcggat gcagtcggcg cgtgtcactg
     6781 taacgacgaa ttaaggtatt acggtcggca tccaggaaca ggagttgcgg tgagaaagcg
     6841 tcaggcaggt tactcatcgc ctgttcgaat atttctggtg actccggcat attacgaaca
     6901 tcaatgctga cggcggcaga aatctcgcga tcggccaggg ttcgagccag atcgggtaac
     6961 aacactacgg gaaggttatc cacgcagtaa aaacccatat cttccagcgc acgcagggcg
     7021 acagatttac ctgaacctga acgtccgctg acgatcatca gtaccatgta ctgtttctcc
     7081 tcacaacgtc taaaagagac attaccgaat aactacgctt catccggagt accttcggta
     7141 tccgtaatga tttgatacag ctcttcatcg ctctgggctg cgcgtaaacg gcggcagatg
     7201 gttttgtccg ccagacgttt cgccaccagc gacagagtat gcaggtgcgt tttagtttgg
     7261 tctgccgaca ccagcagggc aaaaagtaag tccaccggtt ggttgtcgat ggcatcgaaa
     7321 gctataggcg tttcgagctg aacgaaaacg ccaacggcgc gcagagtatc ttcttccagt
     7381 ttgccatgcg gaatggcaat accattgccg ataccggtac tgcccatttt ttcccgcgtc
     7441 aggatagctt caaaaaccac ctgaggcggc aggctaagtt gtttcgccgc cagctcgctg
     7501 atgatttcca gggcgcgttt tttgctctga cagtggacgc ggcttcgcgt acattccctg
     7561 ttaagaacac tgctaagctg tagagttgta tcattatttg tcataatttc acctaagaac
     7621 ctgcccactc aaacagtgga acaggctcgg agcattaacc gtacaaatgg cccgttgtgc
     7681 cgcacaacag gccgccatgc acatgctaat tgcccggaca attagtgttg tttcagttta
     7741 tctttatgtt tggtcaactg gcgtgccagc ttatcaatta agccatcaat ggcagcgtac
     7801 atgtcctgac cttccgcgct ggcatgaatt tcaccaccgt ttacatgcag tgttgcatct
     7861 gaggtgtggg tgactttctc cactttcaga acaacataga cctggttgat tcggtcaaaa
     7921 tattgctcaa gtttggcaaa tttggctgta acaaattcgc gcagtgcctc ggtgatctcg
     7981 acgttatttc cggtaatgtt gagctgcata gtgtcttcct tatcggttgg gtcaaacgag
     8041 ctgtttacgc tggtttgacg gcggaatgga taaagactct cggtacttcg caacagtgcg
     8101 gcgtgccacc atgatacctt gttccgacag caaagaggtt aacttgctgt cgctcaacgg
     8161 tttcgctggg ttttccgccg cgattaattt cttcaccagc gcacgaatcg ccgtggagga
     8221 agcttcgccg ccgccctcgg tattgatgtg actggaaaag aaatacttca gttcaaaaat
     8281 gcctcgtgga ctatgcaggt atttttgcgt ggtcacgcga gatatcgtcg attcatgcat
     8341 ttcgacagcc tgggcgatat cggccagtac catcggtttc atatattctt caccttgctc
     8401 aaagaaggct tgctgctgtt caacgataca gcgactcacg cgcagtagcg tatcgttacg
     8461 gctttccaga ctcttgatca accatttggc atcctgcaga ttgctgcgga taaactggct
     8521 gtcaccatcg ttgcgcgcgt tattgcacat cgaggcgtag tgctggttga tttgcagacg
     8581 cggaatgctg tcactgttga gttctaccgt ccagtgaccg ttatgcttac gcaccagcac
     8641 atctggaatg acatactcag gttcgccagt ctggatcgac tgcccggggc gcggatcgag
     8701 cgactggatc agattgacgg cttctttcag cacatcttct ttcagacgcg tgacgcgcat
     8761 taaagtgcgg aagtcgtgat tggctaacag atcgagatga tcgctaatga tcagtctggc
     8821 ctcttccagc cacggcgtgg ttttatcgaa ttgggagagt tggattagca ggcagtcacg
     8881 cagatctttt gccgctacac cgaccggatc aaaccgttgg atccgcttaa ggacggcttc
     8941 aacctcgtcg atgtcgatct cttcatcgcc catactttcg agaatatctt ccagcgggac
     9001 agtcagataa ccgatgtcat caacggcatc gacgatagag gtagcaatag cgcggtcagt
     9061 gtcggaaaac ggtgtcagct cgacctgcca catcaggtaa tcctgcaagg tctgcgtcgt
     9121 ttcgccctga tagaccggca gctcatcgtc aatgtagtca ccgctggtgc cggatggtgt
     9181 accagcggtg taaatggtgt cccaactggc atcgagcggc agctcttccg gcatctcttt
     9241 ttgttcgagc gcgtcggcgg tgtccagcgt ttcactgtct tgcgtttcgc gggtgtcgat
     9301 ttcttcatga gtgtcgattt gctcaagcag cggattactc tccagcgcct gctgtagctc
     9361 ctgctgaagt tccagcgtcg acaactgcaa cagacgaatt gcctgttgga gctgtggcgt
     9421 catcgccagt tgttggctaa gcctgagttg caaaccttgc ttcatgttca gaatcgtact
     9481 ctcctgctaa aacgtcgcaa acttctaccc tatcagagtc tgaagtcttc cccaaggtat
     9541 acacgcttaa cgtgttcgtc ttgtaagatt tctgtaggcg tgccgtgggc gatcaaatgc
     9601 ccctgactga cgatataagc gcgttcacaa accgccagtg tttcacgcac gttgtggtca
     9661 gtgatcagca cgcccaggcc gctgtcgcgc aggtgctcaa tgatgcgttt aatgtcgata
     9721 accgagatcg ggtcaacccc ggcaaacggt tcgtcgagca gaataaattt cggattcgca
     9781 gccagtgcgc gggcaatttc tacacggcga cgttcacccc cggagagtga ctgccccatg
     9841 ctgtcacgca ggtgctcaat gtgaaactct tccatcagct cgttcgcgcg gtcttcacgt
     9901 tgttcagcag acaagtcgtc acgaatttgc agtaccgcca tcaggttatc gtaaacgctg
     9961 aggcgacgga aaatggaggc ttcctgtggc agatagccga taccgcggcg cgcgcgtgca
    10021 tgcagaggca gcagactgat atcgtcatca tcaataatga tgttgcccgc atcgcgcggc
    10081 acaatgccta caaccatgta gaaagtggtg gtcttaccgg caccgtttgg ccccagcaga
    10141 ccgacaattt ccccggagtt gacggtcagg ctgacgtctt ctaccacgcg acggccttta
    10201 taggcttttg caaggttctt tgcagttaat gttgccataa cgaattaatt acccttcttc
    10261 tgtgccgggg tctggccttt gttgtttttg tcctgcagct gcgacggcac cagaacggtg
    10321 gttacgcgct tgcctttgtc gctgaaagcc tgcattttct gctcttttac caggtaagtg
    10381 atcttatcgc ccttaatgtt gctatcgacc tgctgcagat aagcattacc cgtcagaacg
    10441 acaaaatctt ttgccagttc gtagtgcatc tgggaagcgt gaccttcaac gggtttaccg
    10501 ttgtcctgca tctggtagaa cgttgccggt ttaccgtagc cgtcaatcac ttctttacct
    10561 tgttcgccgc ccggacgggt aacgaccact ttgtcggcat taattttgat ggtgccctgg
    10621 gtgacgatga cattaccggt aaaggtaacc acgttgcctt gcatatcaag agattgctgg
    10681 tccgattcaa tgtggatcgg ctgatcagtg tctccggtta cggcaaatgc cggaatgctg
    10741 gcggccagaa gtgagctggc aagcacaaga ttaaggctga gtttgtttgt tttgaatttc
    10801 ataggatgtt ctaacctttt caatcagctc ggcgttcttg ctgcgtaagt tgccgcgcat
    10861 tttcagaccg ctggagttaa atgttgttcc gtataacgtg acgaggtctt cagaggtaac
    10921 atcctgcgtc accagattga tctgcgcgtt atccgtcgtg attctgcgaa gttgagagtt
    10981 tggcacaagt gcgttgactt caacgtgtcc atataaatag agcatccggt cattggtcag
    11041 cttggcttta tctgctttta cggaccatgt cgggatttta tccttatcaa acgtggtaag
    11101 taccggctgc gtaaaccacg aaacggcctg atcggaataa tattcaacgt gttgagcaat
    11161 caatcgatag cttagtgccc cttctgggtt atagacgagc gtgtccgtat gctcgctttt
    11221 ataggtggga tcattgttgt tgacgaccac ctgggcggta tcgtcttttt cggccatatc
    11281 aatgccgatc atcaccagaa ccgccagtga tagcacaatg ataacccaac gtctggcttt
    11341 actcatatcg attgcccttt ggcttcatcc agtttgccct gcgccaggag taataagtcg
    11401 caaacttcgc gcactgcgcc acgaccgcca gcaatgcgcg tcacgtaatc ggcgcgcggg
    11461 atcaacagtg gatgcgcatc ggccacggcg acgcttaaac ccactttttc cattaccggc
    11521 cagtcgatga gatcatcgcc gacataagcc acattttccg gggcaatcgc cagtttttcc
    11581 agcagatcgc taaaggcgat cagtttgttt gactgcccct gatacaagtg agtgatcccc
    11641 aatgtggcac aacgatcttc taccagttta gcctttcgcc cggtaatgat agcgacttca
    11701 atatcagagg tgagcgcaca acgaatgcca taaccgtcac gaacattgaa cgctttcagc
    11761 tcttcgccat tattgcccat ataaatcagg ccatctgaca gtacgccatc gacatcgagg
    11821 atcagcagac gaatgttctc tgcttttgct ataacgtcgg cgctgacagg gccgtaacag
    11881 gtcgcaagcg acgcacctgc tttgctcatt gttgtttatc cttgaatctt tacactacgc
    11941 ctgcacgcag taaatcatgc atatgtaaca ctccgagtaa atggtcgcca tcggcaacca
    12001 tcacggaggt gatatggcgg gactgcatta agttcagtgc ctcaacggcc agaatgccag
    12061 ggcgcacacg tattcccccc ggcgtcatca catcggcaat acttaactga cgaacatcca
    12121 cgcccatatc gaagacacgg cgtaaatcac cgtcggtaaa gatgccttca atcatcatat
    12181 tgtcatcgca aatgacagtc ataccaagat ttttgcgggt aacttccagc aacgcgtcac
    12241 gcagactggc cgttttctta acatgcggga tctcatcgcc cgtatgcata atatcgttta
    12301 cgcgcagcag aagtttacga cccagtgcgc cgcctgggtg tgagagcgca aaatcttcag
    12361 cagtaaagcc gcgtgctttt aacagcgcga cagcgagggc atcgcccata accagcgtgg
    12421 cggtggtgct gctggtcggt gccagcccta acggacaggc ttctttcgct actttaacac
    12481 acagatgcac atctgcggcg cgcgccatgc tgctctccgg gcgaccggtg atgcagatta
    12541 acggtacgtg aagacgctta agcactggaa ttaaggccgt gatttcgctg gattcacctg
    12601 agttagagat agcaatcacc acatcctgtg gggtgaccat gcctaaatca ccatgcgcgg
    12661 cttcaccagg atggacgaaa aatgaaggtg taccggtgct ggcaaacgtt gcggccattt
    12721 tgcgcccgat gtgccccgat ttccccatcc ccatgacgac aactttccct ttacaccaga
    12781 acatcttttc acaggcaagc gtgaaattct gattgatgta ttgatcaagc tccgccaggc
    12841 attcacgttc aatcgccagg acttctttac ctgcttgctg aaagtcaaaa cccggttgta
    12901 actctacgtg cgacataatg cgtttccagt tattcaacga gtattggcga taaccagtac
    12961 aacatcgcca gccatacgat aaatccgcca gttaataata cccctacacc acggcccggt
    13021 tgcggggagc gccgccagca cagcaacgca aaaataatgc tcaccagcaa catcacgctg
    13081 tagtcacgac tgtacgccaa tggctcaatc tctcctggcg ttatcagcgc gggtaaaccc
    13141 aacacgatga caatattaaa aatgtttgcg ccaatgatat ttccgacagc aatgtcgttt
    13201 tcacctttgc gaacccccgc tattgcggtt gccagttccg gcaggctggt tccgatagca
    13261 attgccgtca gacccatcgt caactcgctg atggcaaagt aattcgccag caccgtggcg
    13321 ttatcaacca ccatccgcgt ggccactggc atgatgataa gcgcaatgcc gagccataaa
    13381 aacgcgacgg gcaatccgcc gtcacgcggc agctctgcaa gctgctctct ggtcaggctg
    13441 tcagtcccct gacgttcagc ctgacgtgca agtttaacaa tgaacagcag ccatagcaca
    13501 gccagaaaga ggagaaagat accatcgctg cgactaagtt gtccgtcata gagtacggaa
    13561 ccggccacta cgctgaccaa caacattaag ggtaattcac gacgcagaac atcggaatgg
    13621 acggtaaaag gacgaaccag cgcagccaga ccgaggatca gcaaaatatt gataatgttt
    13681 gagccgaggg ctgtaccaac ggctaaatcg cgttgttcgt gcagagacgc ggcaagcgag
    13741 acgatgattt ctggtaacga tgtaccaata ctgaccaccg tcatgccgat gatcagcggc
    13801 gggatgccaa aggttcggca aagaatagac gcggcaaaaa ccaggcggtc ggcactgtaa
    13861 acgaccaaaa gtaaaccaac aattaacagt gccgtagcta aaagcatcta acgtcctttc
    13921 ttcaggtata ctcgtcggtc cgctgaagat tttcagaaag ccgtaacgga ttcttaattt
    13981 tgactttatg cggctaaaaa gtaaaacaaa tgccagcttt cgctaaccac ggcgggtaat
    14041 attctgtaaa tatgttgggt tcaaggttaa attgagcgcc atgcttagaa aatcaacgca
    14101 agacgaaggg tgaattatgg agcagtctgt ggcgaattta gtcgatatgc gcgatgtcag
    14161 ttttacgcgt ggcaatcgct gcatcttcga taatatttcc ctgaccgtgc cgcgagggaa
    14221 gatcacggcg atcatggggc catcgggcat cggtaaaacg acgctactcc gtctgattgg
    14281 cgggcaaatc gcaccagatc atggtgagat ccttttcgat ggtgagaata ttccggcgat
    14341 gtctcgttcg cgcctgtata cagtgcgcaa acggatgagc atgttatttc agtccggggc
    14401 gttgttcact gatatgaacg tatttgacaa cgtcgcctat ccactgcgcg aacataccca
    14461 actacccgcg ccattgttgc atagtacggt gatgatgaag ctggaggccg tggggctgcg
    14521 tggagcggct aaactaatgc cttctgaact ttccggtggg atggcgcggc gtgcagcgct
    14581 ggcgcgtgcg attgcgctgg agccggatct catcatgttt gatgaacctt ttgttgggca
    14641 agatcccatc accatgggcg tactggtgaa gctgatttct gagctgaaca gcgcgctggg
    14701 cgtgacttgt gtggtggttt ctcacgatgt gccggaagtg ttaagtattg cggatcacgc
    14761 ctggatcctg gcggacaaaa aaattgtcgc tcatggcagt gcccaggcgt tgcaggcgaa
    14821 tcctgatccg cgcgtacgtc agtttctgga cgggatagct gacgggcctg ttccgttccg
    14881 ctatcctgcc ggcgattatc acgctgatct tttaccaggg agttaagcca ctcatgctgt
    14941 taaatgcgct ggcgtcgctc ggacataaag ggattaaaac cctgagaacg ttcgggcggg
    15001 ccgggttaat gttattcaat gcgctggtcg gcaaaccgga atttcgcaaa catgcgccgc
    15061 tgctggtgcg ccagctctat aatgtcggcg tcctgtcgat gctgattatt gtggtttctg
    15121 gcgtgttcat cggaatggtg ttggggctgc aaggttatct ggttctgacc acttatagtg
    15181 cggaaaccag tctgggtatg ctggtggcgt tatcgctact gcgtgaactg gggccggtgg
    15241 ttgccgcgtt gttgtttgcc gggcgtgctg gttcggcgct aaccgcagaa atcggcctga
    15301 tgcgcgctac agagcaactc tccagtatgg agatgatggc ggtggatccg ctgcgtcggg
    15361 ttatttctcc ccgtttctgg gctggggtta tttcattacc actgttgacg gttattttcg
    15421 tcgccgtggg gatctggggc ggatctctgg tcggcgtcag ttggaaaggc attgatagcg
    15481 ggttcttctg gtcggcaatg caaaatgccg tcgactggcg tatggatctg gccaactgtc
    15541 tgattaagag cgtggtgttc gccatcacgg tgacgtggat ttcgttgttt aacggctacg
    15601 acaccatccc gacgtctgcc gggattagcc gggcaaccac tcgcaccgtt gtccactcgt
    15661 ctctggctgt tctggggctg gattttgtgc tgaccgcatt gatgtttggg aattgagttc
    15721 atgcaaacga aaaaaaatga aatttgggtg ggtatctttt tattagcagc actgctggcg
    15781 gcgctgtttg tttgcctgaa ggcggcgaac gtgacgtcca tacgtactga accgacctac
    15841 acgctttatg cgacgttcga taacattggc ggcctgaaag cgcgctctcc ggtcagtatt
    15901 ggtggcgttg ttgtgggtcg ggtggcggat attacgctgg acccgaaaac ctatctgccg
    15961 cgcgtaacgc tggaaattga acaacgttat aaccacattc cagataccag ttcgctgagc
    16021 attcgtactt ccggcctgct gggggaacaa tatctggcat taaacgtcgg ttttgaagac
    16081 ccggaactgg ggactgctat cctgaaggat ggcgatacaa ttcaggacac taagtctgcg
    16141 atggtgctgg aagatctcat tggtcagttc ctttacggta gtaaaggcga tgacaataag
    16201 aatagtggcg atgcgccagc tgctgcgcca ggtaataatg aaaccactga acctgtgggt
    16261 acaacgaaat aatttcagga gaaccgacgc atgtttaaac gtttaatgat ggtcgctttg
    16321 ctggtgattg cacctctgag tgcggcaacc gcggcagacc agaccaatcc ttataagctg
    16381 atggacgagg cggcgcagaa aacgttcgat cgcctgaaga atgagcaacc gcaaattcgg
    16441 gccaacccgg attatctgcg taccattgtt gatcaggaac tgctgccata cgtacaggtg
    16501 aaatacgccg gtgcgctggt gctgggccag tattacaaga gtgcgacccc tgctcaacgt
    16561 gaagcctact ttgccgcttt ccgtgagtac ctgaagcagg cttacggtca ggcgctggcg
    16621 atgtatcacg gtcaaaccta tcagattgcg ccagaacagc cgctgggcga taaaaccatt
    16681 gtgcctattc gcgttaccat tattgacccg aatggccgtc cgccggtgcg tctggacttc
    16741 cagtggcgta aaaactccca gacgggaaac tggcaggctt acgacatgat tgctgaaggc
    16801 gtcagtatga tcaccaccaa acaaaacgag tggggaacgc tgctgcgtac caaaggtatc
    16861 gacggcctga ctgcgcaact gaaatcgatt tctcaacaga aaatcactct ggaagagaaa
    16921 aaataatgag cgagtcactg agctggatgc agacgggtga cacgctggcg ttatccggag
    16981 agctggatca ggacgttctg ctaccgcttt gggaaatgcg tgaggaagcg gtgaagggga
    17041 ttacctgcat cgatcttagc cgtgtgtccc gcgtggatac gggtggactg gcactgctgc
    17101 tccatcttat cgatctggcg aaaaagcagg gcaacaacgt gacgcttcag ggggtaaacg
    17161 acaaagtgta taccctcgcg aaattgtata atttgcctgc tgatgttctg cctcgttaaa
    17221 ttttttcagg ccggatatgg cgttaaccgc ctgtccggca aacctgcctg caagtcgagc
    17281 cttgtctgat ttatcagcga ggctttttgc ttatttatgc cagcgcaact ttgctctaag
    17341 atgtttcgct ggtttattaa ctgatgattg aagatcccat ggaaaataat gaaattcaga
    17401 gcgtgttgat gaacgctctc tccctccagg aagtccacgt ttccggcgat ggcagccact
    17461 ttcaggttat tgccgtgggt gagttgtttg acggcatgag tcgggttaaa aaacagcaga
    17521 cggtctatgg cccgctgatg gaatatattg cggataaccg cattcatgct gtgtcgatca
    17581 aagcgtatac ccctgcggag tgggcgcgcg atcgcaaact gaacggcttt tgagctatgg
    17641 gcgattcgcc ggtagcggat atgaattgtt aactgagaac aaactaaatg gataaatttc
    17701 gtgttcaggg gccaacgaag ctccagggcg aagtcacaat ttccggcgct aaaaatgctg
    17761 ctctgcctat cctttttgcc gctctactgg cggaagaacc ggtagagatc cagaacgtcc
    17821 caaaactgaa agacgtcgat acatcaatga agctgctaag ccagctgggt gcgaaagtag
    17881 aacgtaatgg ttctgtgcat attgatgccc gcgacgttaa tgtattctgc gcaccttacg
    17941 atctggttaa aaccatgcgt gcttctatct gggcactggg gccgctggta gcgcgctttg
    18001 gtcaggggca agtttcactg cctggcggtt gtacgatcgg tgcgcgtccg gttgatctac
    18061 acatttctgg cctcgaacaa ttaggcgcga ccatcaaact ggaagaaggt tacgttaaag
    18121 cttccgtcga tggtcgtctg aaaggcgcac atatcgtgat ggataaagtc agcgttggcg
    18181 caacggtgac catcatgtgt gctgcaaccc tggcggaagg taccacgatt attgaaaatg
    18241 cagcgcgtga accggaaatc gtcgataccg cgaacttcct gattacgctg ggtgcgaaaa
    18301 ttagcggtca gggcaccgat cgtatcgtca tcgaaggtgt ggaacgttta ggcggcggtg
    18361 tctatcgcgt gctaccggat cgtatcgaaa ccggtacttt cctggtggcg gcggcgattt
    18421 ctcgcggcaa aattatctgc cgtaacgcgc agccagatac tctcgacgcc gtgctggcga
    18481 aactgcgtga cgctggagcg gacatcgaag tcggcgaaga ctggattagc ctggatatgc
    18541 atggcaaacg tccgaaggct gttaacgtac gtaccgcgcc gcatccggca ttcccgaccg
    18601 atatgcaggc ccagttcacg ctgttgaacc tggtggcaga agggaccgga ttcatcaccg
    18661 aaacggtctt tgaaaaccgc tttatgcatg tgccggagct tagccgtatg ggcgcgcatg
    18721 ccgaaatcga aagcaatacc gttatttgtc acggtgttga aaaactttct ggcgcacagg
    18781 ttatggcaac cgatctgcgt gcatcagcaa gcctggtgct ggctggctgt attgcggaag
    18841 ggacgacggt ggttgatcgt atttatcaca tcgatcgtgg ctacgaacgc attgaagaca
    18901 aactgcgcgc tttaggtgca aatattgagc gtgtgaaagg cgagtaatcg tctgagagct
    18961 ggcagccccg ttcgcggggc taccgccaga ccactcattt tttcggttta gtgtagctac
    19021 gcatcaactg cgttctgtcg ataaactcat gggtctgcgg atcatggtag cgtgatggcc
    19081 agataaccca ggggtcagtt cccagggctt tcgcaataat catctctcct ttcggccatg
    19141 ggcgcgataa tgcgtttgcc agcgttgagg aactcaaacc atttctgcga gactccgccg
    19201 ccattgacgt tcccttcttg cgcaaacccg caatgatgtc agcgggatgc cagtcaatga
    19261 aattactttc catctttcct cactccttaa gttagtcgct ttcattcaga acacttcttc
    19321 tgtcgggcat cactatactc ctgatggcct attgctctaa aaagttcctg taaagagtgt
    19381 gaatattcag aatatataaa gattcatcgc cctttttttt caggtgttat agcgctgaat
    19441 tagtgtttt
//
LOCUS       DAZOYL010000050        19316 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZOYL010000050 DAZOYL010000000
VERSION     DAZOYL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.050"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..507
                     /locus_tag="ACHUOY_001300"
     CDS             <1..507
                     /locus_tag="ACHUOY_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0682128.1"
                     /translation="LSAGDTYNLIKTLDVCHYDSETKVLTSHHGIIRNEENECYCLGA
                     LQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRSNAVASTPVYENYFNNTTAF
                     RPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVP
                     GLSCFQPQ"
     gene            769..2391
                     /locus_tag="ACHUOY_001301"
     CDS             769..2391
                     /locus_tag="ACHUOY_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0682129.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2581..2694
                     /gene="yhiY"
                     /locus_tag="ACHUOY_001302"
     CDS             2581..2694
                     /gene="yhiY"
                     /locus_tag="ACHUOY_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR0682130.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2757..3824
                     /locus_tag="ACHUOY_001303"
     CDS             2757..3824
                     /locus_tag="ACHUOY_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0682131.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            3821..6556
                     /gene="rbbA"
                     /locus_tag="ACHUOY_001304"
     CDS             3821..6556
                     /gene="rbbA"
                     /locus_tag="ACHUOY_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR0682132.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6556..7680
                     /locus_tag="ACHUOY_001305"
     CDS             6556..7680
                     /locus_tag="ACHUOY_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0682133.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7753..8026
                     /locus_tag="ACHUOY_001306"
                     /pseudo
     CDS             7753..8026
                     /locus_tag="ACHUOY_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            8023..8382
                     /locus_tag="ACHUOY_001307"
     CDS             8023..8382
                     /locus_tag="ACHUOY_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR0682134.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8502..8903)
                     /gene="nikR"
                     /locus_tag="ACHUOY_001308"
     CDS             complement(8502..8903)
                     /gene="nikR"
                     /locus_tag="ACHUOY_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR0682135.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(8909..9715)
                     /gene="nikE"
                     /locus_tag="ACHUOY_001309"
     CDS             complement(8909..9715)
                     /gene="nikE"
                     /locus_tag="ACHUOY_001309"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR0682136.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(9712..10476)
                     /gene="nikD"
                     /locus_tag="ACHUOY_001310"
     CDS             complement(9712..10476)
                     /gene="nikD"
                     /locus_tag="ACHUOY_001310"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR0682137.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(10476..11309)
                     /gene="nikC"
                     /locus_tag="ACHUOY_001311"
     CDS             complement(10476..11309)
                     /gene="nikC"
                     /locus_tag="ACHUOY_001311"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR0682138.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(11306..12250)
                     /gene="nikB"
                     /locus_tag="ACHUOY_001312"
     CDS             complement(11306..12250)
                     /gene="nikB"
                     /locus_tag="ACHUOY_001312"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR0682139.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12250..13824)
                     /gene="nikA"
                     /locus_tag="ACHUOY_001313"
     CDS             complement(12250..13824)
                     /gene="nikA"
                     /locus_tag="ACHUOY_001313"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0682140.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(13935..14522)
                     /gene="acpT"
                     /locus_tag="ACHUOY_001314"
     CDS             complement(13935..14522)
                     /gene="acpT"
                     /locus_tag="ACHUOY_001314"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR0682141.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(14577..15626)
                     /locus_tag="ACHUOY_001315"
     CDS             complement(14577..15626)
                     /locus_tag="ACHUOY_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0682142.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            15758..16975
                     /locus_tag="ACHUOY_001316"
     CDS             15758..16975
                     /locus_tag="ACHUOY_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0682143.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(16979..17536)
                     /gene="dcrB"
                     /locus_tag="ACHUOY_001317"
     CDS             complement(16979..17536)
                     /gene="dcrB"
                     /locus_tag="ACHUOY_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR0682144.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(17609..18274)
                     /locus_tag="ACHUOY_001318"
     CDS             complement(17609..18274)
                     /locus_tag="ACHUOY_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR0682145.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            18495..18740
                     /gene="tusA"
                     /locus_tag="ACHUOY_001319"
     CDS             18495..18740
                     /gene="tusA"
                     /locus_tag="ACHUOY_001319"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR0682146.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(18842..>19316)
                     /locus_tag="ACHUOY_001320"
     CDS             complement(18842..>19316)
                     /locus_tag="ACHUOY_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFR0682147.1"
                     /translation="GVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQ
                     HAPLAMVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARA
                     THANIRQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
BASE COUNT         4633 a         4900 c         5228 g         4555 t
ORIGIN      
        1 ctctctgccg gtgatacgta taacttaatt aaaacgctcg atgtttgtca ttatgactca
       61 gaaacgaaag ttttaaccag tcatcacgga ataataagaa atgaagaaaa tgaatgttat
      121 tgtctcggtg ctttgcaggt tccatttaat cagatgaaag acccaattga tcctgaggaa
      181 ttagcaaata tatttaataa aaaacataaa caacacatgg atgatcgtct tatccatcta
      241 attagaagca atgctgtagc atccacacct gtatatgaaa attattttaa taacactact
      301 gctttcagac caaaacctga agatatcttc aaatgtggac agacgctcaa aaaaactaaa
      361 cagaaatatg gccattatgg attaggggta gatcagcatc aaaaaattga taattatacc
      421 atggggctaa attcctggaa gatagcacct aacgaacgtg gtgataaaaa aggtgtccca
      481 gggcttagtt gttttcaacc tcaataaaac ttcatgagtg ctgtggtatc tctttttaat
      541 tttgccagta tttcataggt tttcttaatc gattgtagag gcatgttctg atgacgcctc
      601 tacaatcttt agggactttc tctgctatga gataagaaat ttcatattat gctttaaggc
      661 tgaatgattt gtgcaaaata taattatttt cccatcgaac atgaggtgta tgtcataaga
      721 ttaaatgtta actatgtatt cctcgcggta aataatatga ggtaaaagat gaaaattgga
      781 actgtggcag gcactaacga cagtacaacg acaatcacaa caaatgatat ggtgcaagag
      841 catgttacca acttcactaa agaactgttt gggtatattg caaatggtat aggtgatgat
      901 atatcaagta ttgccaggac aatgctcggt gaggttgtgg aaaaaataga tgattggcaa
      961 atagaacggt tccaacaatc tattcaagat gacaaaattt catttaccat acaaacgaat
     1021 cattcagaaa aatatagtat gctatcgggc atgcgcgctc atattctacg taggaataac
     1081 tgttatcagt ttattgtgac aattaatagt aaaaattacg gttgccctct tgataatacc
     1141 gatattaatt ggtgcagcat tgtttattta cttaataata tgacagtaaa tgataatgct
     1201 aatgatgttg cagttactga gtcgtacaaa cccatctgga actggaaaat atcgcaatac
     1261 aatgtatttg atattaaatt cgaaacaatg ataaaacccc agttcgctga cagaacctat
     1321 ttctccaatt gttcacctgt tgatcccaca agtaccagac cgacctattt tggcgatacc
     1381 gatggctcag tcggtgcagt tttgtacgcg ctttttgcaa cgggacattt gggaatcatg
     1441 gctgaaggag aaaatttcct tagccagttg cttaatatag aagatgaagt tctcaacgta
     1501 ttgctaaggg agaactttaa tgagcaactt gacactaatg ttaatataat aatcagtatc
     1561 ctgaacagaa gagacattgt tttagaaagt cttcagcctt atttggttat taataaagac
     1621 gcagtcacgc catgtacgtt tctgggcgac cagactggcg atcgatttag taatatctgt
     1681 ggcgataaat ttatcatcga tttattaaaa cgcataatga gtatcaatga aaacgttcat
     1741 gtattagctg gaaatcatga aacgaattgc aatggtaact atatgcagaa cttcacgcgc
     1801 atgaaaccac ttgatgagga tacctactct gggataaaag attatcctgt atgtttctat
     1861 gaccccaaat acaaaataat ggcaaatcat catgggataa cttttgatga tcagcgaaaa
     1921 cgttacatta taggaccgat aacagtctct agtgatgaaa tgaccaatgc tctcgatccg
     1981 gtcgaattag cggcaattat caataaaaag caccatgctg taattaatgg caaaaagttt
     2041 aaaaatagcc gggctatctc ttgtcgatcc tttaatcgtt atttttctgt ttcgacagat
     2101 tacagaccta aacttgaagc actattagca tgctcacaaa tgttaggtat aaatcaagtt
     2161 gttgcacata acggtaatgg tggaagagaa tgtataggtg aaacggggac agttttaggt
     2221 cttaacgccc gtgacagtaa acatgctgga agaatgttca gcatgcataa ttgccaaatt
     2281 aaccctggtg cagggcctga gataaccact ccctggaagt cttaccagca tgaaaaaaac
     2341 agaaatggac ttatgccttt gatcagaagg cgcacaatgt tacagctctg aaccatagac
     2401 aacatgaata ttaatgttac tatctggttg agactgttga taaaacgtaa aaaggaatgc
     2461 tctgttcctg caactgaaca ggcgtgaaac gctatccaac aggatggata ccgttttgtc
     2521 agccagtttt atagatcaca cttatgagta tgcgtgtttc atttacggcc aggaatgctg
     2581 atgatgacaa cattactacc cgttttcact aagccttccc cgttggcgct caatgctctg
     2641 cgcgctggtc gtatttgtcg tttccttctt atcccggatg gaagaatccg ctgaacgtcg
     2701 ttcggcggca gttgtaacac gcgtcgtttg cttgcaggga ttatggcggg tcgattatgg
     2761 ataagagtaa gcgccatctg gcgtggtggg ttgtcgggtt actggtggtg gctgccgtcg
     2821 tggcgtggtg gctgttgcgc ccggcgggtg tgccggaagg ctttgctgtc agcaatgggc
     2881 gcattgaagc gacggaagtg gatatcgcca gcaaaattgc cgggcgtatc gacaccattc
     2941 tggtgaaaga aggccagttt gttcgcgaag gcgaagtgct ggcgaagatg gatactcgcg
     3001 tgttgcagga gcagcgactg gaagccatcg cgcaaatcaa agaagcacaa agtgccgttg
     3061 ctgccgcgca ggctttgctg gagcaacggc aaagcgaaac tcgtgccgcg cagtcgctgg
     3121 ttaatcaacg ccagtccgaa ctggactccg tggctaaacg tcatacgcgt tcccgctcgc
     3181 tcgcccaacg aggggctatt tctgcgcaac agctggatga cgatcgcgcc gccgctgaga
     3241 gcgcccgagc tgcgctggaa tcggcaaaag ctcaggtatc ggcttctaaa gcggctatag
     3301 aagcggcccg taccaatatc attcaggccc aaactcgcgt cgaagccgcg caagccactg
     3361 aacggcgtat tgccgcagat atcgatgaca gtgaactgaa agccccgcgt gacggacgcg
     3421 tgcagtatcg ggttgctgag ccaggggaag tgctggcggc aggcggtcgg gtgctgaata
     3481 tggtcgatct cagcgacgtc tatatgactt tcttcctgcc aaccgaacag gcgggcacgc
     3541 tgaaacttgg cggtgaagcc cggctgatcc tcgatgccgc gccagatctg cgtattccgg
     3601 cgaccatcag ttttgtcgcc agtgtcgccc agttcacgcc aaaaaccgtc gaaaccagcg
     3661 atgaacggct gaaactgatg ttccgcgtca aagcgcgtat cccaccggaa ttactccagc
     3721 agcatctgga atatgtcaaa accggtttac cgggcgtagc gtgggtgcgg gtgaatgaag
     3781 aactttcgtg gcctgacgac ctcgtggtga ggttgccgca atgacgcatc tggaactggt
     3841 tcccgtcccg cctgtcgcgc aactggcggg cgtgagccag cattatggaa aaaacgttgc
     3901 gctgaacaat attactctcg atattccggc ccgctgtatg gtcgggctga ttggtccgga
     3961 tggcgtcggg aagtcgagct tattgtcgtt gatttccggt gcccgcgtca ttgagcaggg
     4021 caatgtgatg gtgctgggcg gcgatatgcg cgacccgagg catcgccgcg acgtctgccc
     4081 gcgcatcgcc tggatgccgc aggggctggg caaaaacctc taccacacct tgtcggtgta
     4141 tgaaaacgtc gattttttcg ctcgcctgtt cggtcacgac aaagcggagc gggaagtgcg
     4201 aattaatgag ctgctgacca gcacggggtt agcaccgttt cgcgatcgtc cggcagggaa
     4261 actctccggc gggatgaagc aaaaacttgg gctgtgctgc gcgttaatcc acgacccgga
     4321 actgttgatc cttgatgagc caacaacggg ggttgacccg ctctcccgct cccagttctg
     4381 ggatctgatc gacagtattc gccagcggca gagcaatatg agcgtgctgg tcgccaccgc
     4441 ctatatggaa gaggccgaac gcttcgactg gctggtagcg atgaatgccg gagaagtgct
     4501 ggcaactggc agcgccgaag agctacggca gcaaacgcaa agcgctacgc tggaagaagc
     4561 atttataaat ctgttaccgc aagcgcaacg ccaggcgcat caggcggtag tgatcccacc
     4621 gtatcaacct gaaaacgcag agattgccat cgaagcgcgc gatctgacca tgcgttttgg
     4681 ttccttcgtt gccgttgatc acgttaattt ccgcattcca cgcggggaga tttttggttt
     4741 tcttggttcg aacggctgcg gtaaatccac caccatgaaa atgctcaccg gactgctgcc
     4801 cgccagcgaa ggtgaggcgt ggctgttcgg gcaaccggtt gatccaaaag atatcgatac
     4861 ccgccgtcgg gtgggctata tgtcgcaggc gttttcgctc tataacgaac tcaccgtgcg
     4921 gcaaaacctt gagttacatg cccgtttgtt tcacatcccg gaagcggaaa ttcccgcaag
     4981 agtggctgaa atgagcgagc gttttaagct caacgacgtt gaagatgttc tgccggagtc
     5041 attgccgctc ggcattcgcc agcggctttc gctggcggtg gcggtgattc atcgcccgga
     5101 gatgttaatc ctcgatgagc ctacttctgg tgtcgatccg gtggcgaggg atatgttctg
     5161 gcagttgatg gtcgatctct cgcgccagga caaagtgact atcttcatct ctacccactt
     5221 tatgaacgaa gcggaacgtt gcgaccgcat ctcactgatg cacgccggaa aagtgcttgc
     5281 cagcggtaca ccgcaggaac tggttgagaa acgcggagcc gccagtctgg aagaggcatt
     5341 tatcgcctat ttgcaggaag cggcagggca gagcaacgaa gccgaagcgc cgcccgtggt
     5401 acacgacacc acccacgcgc cgcgtcaggg atttagcctg cgccgtctgt ttagctacag
     5461 ccgccgcgaa gcgctggaac tgcgacgcga tccagtacgt tcgacgctgg cgctgatggg
     5521 aacggtgatc ctgatgctga taatgggtta cggcatcagt atggatgtgg aaaacctgcg
     5581 ctttgcggtg ctcgaccgcg accagaccgt cagtagccag gcgtggacac tcaacctctc
     5641 cggttcccgt tactttatcg aacagccgcc gctcactagt tatgacgagc ttgatcgtcg
     5701 gatgcgtgcg ggcgatatca cggtggcgat tgagatcccg cccaatttcg ggcgcgatat
     5761 cgcgcgtggt acgcctgtgg aactcggcgt ctggatcgac ggagcgatgc ccagtcgtgc
     5821 cgaaacggta aaaggttacg tgcaggcaat gcaccagagt tggcttcagg atgtggcgag
     5881 ccgacaatcg acacccgcca gccaaagcgg gctgatgaac attgagacgc gctatcgcta
     5941 taacccggac gtaaaaagcc tgccagcgat tgttccggcg gtgatcccgc ttctgctgat
     6001 gatgatcccg tcaatgctaa gcgcccttag cgtggtgcgg gaaaaagagc tagggtcgat
     6061 tatcaacctt tacgtgaccc ccaccacccg tagcgaattt ttgcttggca aacagctgcc
     6121 gtacatcgcg ctggggatgc tgaacttttt cctgctctgc ggcctgtcgg tgtttgtgtt
     6181 tggcgtaccg cataaaggca gtttcctgac gctcaccctg gcggcgctgc tgtatatcat
     6241 cattgccacc ggaatggggc tgctgatctc cacctttatg aaaagccaga ttgccgccat
     6301 tttcggaacg gcgattatca cgttgatccc ggcgacacag ttttccggga tgatcgatcc
     6361 ggtagcttcg ctggaagggc ctggacgttg gatcggcgag gtttacccga ccagtcattt
     6421 tctgactatc gcccgcggaa cgttctcgaa agcgctggat ctgactgatt tgtggcaact
     6481 ttttatcccg ttgctgatag ccatcccgct ggtgatgggc ttaagcatcc tgctgctgaa
     6541 aaaacaggag ggatgatgcg ccatttacgc aatattttta atctgggtat caaagagttg
     6601 cgcagtctgc tcggtgataa agcgatgctg acgctgattg tcttctcgtt tacggtgtcg
     6661 gtgtattcgt cagcgaccgt tacgccagga tcgttgaacc tcgcgccgat cgccattgcc
     6721 gatatggatc aatcgcagtt atcgaaccgg atcgttaaca gcttctatcg tccgtggttt
     6781 ttgccaccgg agatgatcac cgccgatgag atggatgccg gactggacgc cggacgctat
     6841 accttcgcga taaatattcc gcctaatttt cagcgtgatg tcctcgccgg acgccagccg
     6901 gatattcagg tgaacgtcga tgccacgcgc atgagccagg catttaccgg caatgggtat
     6961 atccagaata ttatcaacgg tgaagtgaac agctttgtcg cgcgctaccg tgataacagc
     7021 gaaccgttgg tatcgctgga aacccggatg cgctttaacc cgaacctcga tcccgcgtgg
     7081 tttggcgggg tgatggcgat catcaacaac attaccatgc tggcgattgt attgaccgga
     7141 tcggcgctga tccgcgagcg tgaacacggc acggtggaac acttactggt gatgccgata
     7201 acgccgtttg agatcatgat ggcgaagatc tggtcgatgg ggctggtggt gctggtggta
     7261 tcgggattat cgctggtgct gatggcgaaa ggtgtactgg gcgtaccgat tgaaggctcg
     7321 atcccgctgt ttatgctggg cgtggcgctc agtctgtttg ccaccacgtc aatcggcatt
     7381 tttatgggga cgatagcgcg ttcaatgccg caactggggc tgctggtgat tctggtgctg
     7441 ctgccgctgc aaatgctttc cggtggatcc acgccgcgcg aaagtatgcc gcagatggtg
     7501 caggacatta tgctgaccat gccgacgaca cactttgtta gcctcgcgca ggccattctc
     7561 taccggggtg ccggattcga aatcgtctgg ccgcagttcc tgacgctgat ggcaattggc
     7621 ggcgcgtttt tcaccattgc gctgctgcga ttcaggaaga cgattgggac aatggcgtaa
     7681 ggaagggaaa cttgacttca tactgtgatg tcatactatt gatgtcataa agtgaagtca
     7741 taaggacatg ccatgagaac caaggtacag gctttacgga agaaaaaaaa atacattgga
     7801 tcaaatattt aaaactcctg ttcctcaagg gatcaagtgg tctgatatag agtcactggt
     7861 taaagcatta ggcggagaaa ttaaggaagg aagaggttcg cgttgtaagt tcatactaaa
     7921 tatgagcgtt gcgtgtttcc atcggcctca tccgtcgcca gataccgata aaggcgctgt
     7981 agaaagcgtg cgtgactggt tactaagtat aggggtaaaa ccatgatcaa actgaagacg
     8041 ccaaactcta tggagattgc cgggcaacca gctgttatta cctacgtgcc agaactgaat
     8101 gcatttcgtg gcaaattcct gggcttgtca ggttattgtg attttgtctc tgacagcatt
     8161 caggggctgc aaaaagaagg ggaactttcc ctgcgagagt atcttgagga ttgtaaagct
     8221 gcgggtattg agccgtatgc gcgtacagaa aagattaaga cctttacgtt acgctatcca
     8281 gaatcgctga gtgaacgcct taataatgcg gctgcgcagc aacaagtctc tgttaatacc
     8341 tatattattg aaacactgaa tgagcgtttg aatcatttat aaattttagc ggcgtacatt
     8401 acgcagaata tctgccttta caggatctat ctatgcaact gaacttaatt ttataaatta
     8461 agttcagtta atatcttttc tttcctcgtt tcagaaataa ctcaatcttc cttcggcaag
     8521 cactgcaaat gcccatgccg cacgccgcgc tgggcgataa cgtcatcggc aaaatgctgc
     8581 acgtcgccca tatcaccttt cagcacggcg atttccagac agtcgtcgtg attgatatgc
     8641 acatgcagcg tggcgacgga gagatcgtgg tgatggtgct gggtggagac aatgcggctg
     8701 gctaagtcgc gtttttcgtg ttcatacaca taggagagca ccgcgaaacc ttgcgtgccg
     8761 tgctgctgag tggcctcctg ggccagggcg ctacgcagaa tatcgcggat agcttcggaa
     8821 cggttgttat aaccacgacg ctggctcagg ctgtccagcg tctccagtaa atcgtcatca
     8881 agcgtgatgg tgactcgttg catttgcgtt aaaccttttc tgtggtgcga cggcgcacgg
     8941 ggaatgcggg taataccgcg ttttgtagca cacgtccggc gtcagaggaa aaggttaatt
     9001 tatctcccac cacctgggtt tcgacgattt gtccgttgtc catcaccatc acccgctgac
     9061 agaagcgttc caccaggcgt aagtcgtggg tgatgaacag gcaggcggtg ccaaactgtt
     9121 gttgtagctt tttcagcagg cgaatgacgc ccgcctgtaa cacgagatca aggttagaaa
     9181 cggcttcatc cagaatcagt agtttcggtt cgaccgccag cgcgcgagcc aggcagacgc
     9241 gctggagctg gccgccgctt aactgcggtg ggcgtttgtc gagaacgctg tcatcgagat
     9301 cgaccgcctt cagcatttcg ctggcgcgcg ccagttgttc ggattttttc agtgatagca
     9361 ggtggcgcat cggttcacgc aggatctcgc gcacggtttt gcgcggattc acggcgctga
     9421 tggagtcctg aaataccatc tgaatatcgc ggcggaacgc tttacgctgg gcgcgattga
     9481 gtttcgccag cggttcgcca cgccagctaa tattcccctg cgcgggcgat tctaaaccca
     9541 ccagcaaccg cgcgagggta cttttcccgc agccgctgcg ccccagcagg gcgacggttt
     9601 cgccgctttt cagggtcagg gaaacgttat tcagcaccgc ctgatgttga tgttttccgt
     9661 taaatccacc gtgggcatag tgatgggaaa ggccggagac gttaagtaaa gtcatgatgc
     9721 cagctccata ccgtagaggg cgagatgagc ggaaaccagg ctgcgcgtta ccgcatgttt
     9781 gggggcgtta aacagcgttt ctacatcgcc ctgttcgaca attttacctt gtgacatcac
     9841 cgccacgtca tccgccagac gcgccacaac gcccatatca tgggtgacca gcagcattcc
     9901 cggcgcttgt ttttgcataa tgctttccag cagatcgagg atgcgcgcct gtgctaccac
     9961 gtcgaggtcg gtggtcggtt catcggcgat gataaacggt gattcacaca gcaccgccat
    10021 cgcaatcatc atgcgctgca acatgccgcc gctcatctcg aacgggtaca gcttcagcac
    10081 gcgcgcggcg ttttccagcc ccaccgcttc tatggcagcg gtaagcgtag cgtcatcggc
    10141 gggtttccct aacgccaggc aggtttcccg cgcgtgggtg tgcatggtgt gtagcggatt
    10201 aaaggcgctg cgcgggttct gcatgatggt ggcaattttg atgccgcgca gggcgcaggg
    10261 ataaaccgct ttgccatcgg ctaaaatttc ccccgccgtc tggcgaacgc cagcgggcaa
    10321 aatgcccagc gtcgcggcgc aggttaatga tttcccgctg ccgctaccgc cgactaacgc
    10381 cagcacgcgc ccgcgttgca gggttaacga tacaccgtgt accagcggct gcgcggcctg
    10441 tagcgcgata ttacgtagtt caatctgttg cggcattagt gtgcgtgctc cgtcaccaga
    10501 tgaggatcca gatgatcgcg cagtgcgtca cccaccaggt taaaggccat cacgctgata
    10561 aacagcgcca gccccggcca gaacatttgc agcggctggg tccagatata ctggcgcgcg
    10621 tcgttaatca tcacgcccca ttcggcggtc ggcgcggtca caccgaggcc aaggaaagac
    10681 atccccgcga cgtgcagcat catatggccg atatccagcg ttgccagcac cagcagcgaa
    10741 gggatcaccg cgcctgccag atgatcgaca aacacccgca catggcccgc accggaaagc
    10801 cgtgacgcca gcacaaactc gcgttggcgt agtgaaatca ccaagctgcg caccatgcgt
    10861 gcataccacg cccagtgcga cagggcgatg gcgataatta cgttggtcag cccggtgccg
    10921 agcacgccaa ccataaagaa cgacagaatc gaggtcggga aggtcataaa catatcggcg
    10981 acgcgcatgg tggcctgatc aacgcgcccg ccaatcaacc cggcgctgcc gccaataacc
    11041 agccctaatg tcagcaccag cagcaggcag gccattaccg aaccgagcga cacgcgggtc
    11101 gctgccatca gccgcgagaa aatatcgcga cctaagtgat cggtgcccag ccagtgctgc
    11161 gcatccggcg aaagcaggcg cgacggcaaa tcaatcgcct gggggtcata cggcagccac
    11221 cactggctgg tgagcgcaat cagcgccagc agggcgataa tgatcagcgc caggcgtacc
    11281 gaccagcggg aagagaggaa aaagttcacg cgtgcgctcc ttcatgacga cgaatgcgcg
    11341 ggtccagcgc ggcgttgagc aaatcgacaa tcaaattaca gaccacaaaa accaccacca
    11401 tcatcagcgt aaagcactga atcaccggat agtcacggtt aaaaatcgcc gacaccgcat
    11461 agcgcccgac gcccggccag gcaaagatgt tttcgataat catcgtcccg ccaatcagtt
    11521 cgccgatgtg catccccacg gcggtgatca tcggcagcga ggcattgcgc agaatatggc
    11581 ggcgttcggt ctgtttgtcg ttcaggccgc gcagacgcgc ccaagtgacg tgacgctgac
    11641 cggcgacgtc cagcatactg gcgcgcagca gtcgcgcgtt aatcgccagc gacataaagg
    11701 caatggaaac cgcaggcaaa atgatgtgct gccagccgcc gtaacccatc gcgggtagcc
    11761 attgcagata caccgaaaac gccatcacca gtaaaaacgc cagccagaag ttaggcatcg
    11821 acacgccaag aaacgcgatg aaacgtacgg cgaaatccgg cagacggtcg cgatggcgcg
    11881 ccgcccagat accgagcggt acggaagtga gcagaattaa taccagcgcc gcacctgcaa
    11941 gttccagcgt ggcgggcagg aagttcagca tatcgtccag caccgggcgt tggctggcga
    12001 atgagatacc aaagtcaaga tgcagcgcct tccacaacca ggtaccgtac tggacgtaca
    12061 gcggctgatc cagcccgagc atggtgcggg tagaggccag catctccggc gtcggcggca
    12121 ggttagataa acgcagataa tcgagcgccg ggtcgccggt accgaggcgc agcatcagaa
    12181 aaatgatcac cgaggcggcg agcaccatcg ggatcagcag cagaaagcgg cgtaatacgt
    12241 aacgcaacat taaggtttca ccggtttaat ctgttcgaac ggaatttcgg tggcgatcgg
    12301 cgcgtagggg atattaccca gctccggttt tgataccacc atcatcgaga tgtagctgat
    12361 aggcagataa accgcctcgt catgcagacg ggtcaaaatg tcgcgataca gcgcctgacg
    12421 ttgcgtttcg tcatgggtcg ccagcacttc gccgatctct ttatcaatca gcggcttgtc
    12481 ggctaatcct tgctgtgcct ggaagtcagc gtgcgacggt acgcgcattg aactcaggaa
    12541 ggcgtgtgga tcgtatggcg cgccccaggt gcggtggaaa atcatgccaa aacgaccgtc
    12601 gcgctgacga gcatagatac tgctctcttc ttcgccaatc agcgagacat ctgcgccaat
    12661 ctggcgcata tcagcctgaa tgatttccgc catcgatttg cttaacgcat cggtgccgat
    12721 gaacgacagt tcaatgcgca gcggctgacc atttttctcg cggatgtctt tgcccgcagg
    12781 cagcgtccaa ccggcttttt ccagcaactc tttcgctttc tgcggatcgt actggcgcgg
    12841 tttcaggccg agattagcgt aaggcacaga aggggcaaac agggtgtcgg cgacctgcgg
    12901 ggtgccatat aacgcgttat caatcagcga ttttttgttt accgcgtaat taagagcttc
    12961 acgtactgcc agctcgttgg tgggggcttt ggcggtattg agcgccagca tcacggtttc
    13021 gatcggctgt gacagttggg tgtggtaagc cggattctgg ctaaagcggg cgaaggtatc
    13081 gagcggtaat aacccttcgt ttccgtacag caggtcgata tcgccagttt caaacgccac
    13141 cgcgcgggta gtcgggtccg ggatgacgtt aaaggtgatc tttttaatcg ctggcttttc
    13201 gccccagtag ttttcgttac ggacgaagac atcgtactga ttcagtttcg attcctgcaa
    13261 aatccacggt ccggtgccaa tcggcgcttt aattccgttc atggtttcat ggtttttaaa
    13321 ctgcgaggga gcgataaaac ggaaaggacg gggcagggcc agttcttgca ggaaaggata
    13381 gtaggcgctt ttcagggtaa tttgcagctc tgttttactg agtgctttaa catcaacaat
    13441 ctggtttgcc agctccagcc aggcgtgacg ttgacggtta tcgagcactg cgcggaagtt
    13501 ttctgccgcc gcctcggcat cgaacggttc accgttggag aatttcacgt catcacgcag
    13561 ggtgaaggtc caggttttac catcttctga atgagtccag ctttttgcca gccacgggat
    13621 caccgaaccg tctgcctgat atttcaccaa tggttcataa accatgctct gggcgaacat
    13681 ctggttaggc gtgtaaaggt gcgggtttag tggcccgaca ttcaccggcc aggcggtagt
    13741 gatttcatct ggtgcagcgg catggacgat aaaagacgca caagccagca gcgcaaatag
    13801 agtgcggcgg agtgtggaga gcatggtaac cccaatggat taaaatagat ggcggaaata
    13861 agtatgacga ttttaagtat tcgtcatact gattacctgt tctggatcaa tagatgggca
    13921 gtcgggtggg cggatcagtt aactgaatcg atccattgca cactgtcggc ggtgagcgta
    13981 aagggggtgg gggtgcagat cgcaaggctt aaattttcta gctgacaatg gctgactgac
    14041 agcgaggagt gataggtgct atctacgctg acgatttgcc aggcgctgcc gccacgctgt
    14101 ttaacgatgg cttctttgcg cgtccagatg cgccagaaca tttccagttg ctgatcagga
    14161 tgcacggcgt ccatttcagc gtgttccccg aggctgaaca cggcgttcgc cagccagcgc
    14221 cagttggcgc gcgggcgaat cacttcgata tcgcagccga cttcgccttc atcgctcaac
    14281 agcagagcga tatcgtcacc gctatggctt aagttgaacc atagcggcgt ttccggcgcg
    14341 aatgcaggtt tgccttgttc gccatagatg atctccggta gcggggaaag cgtgtgcgaa
    14401 agcaatgcac gccccgccag ccagcgttcg cgtcgtggac cttgcggtgc ttgctcgcgt
    14461 aaacccggtg gcagtggagc tgcgcttaag gtcgaaactt tccccagaac tatccgatac
    14521 atatcagggc caacgtttaa tggaaaatga aagtgcgtat cgtatcactt gtcgcctcat
    14581 cccggtaacc gacttttcgg tctgcctggc cccagtaaaa tcgccagttt gctaccgcct
    14641 ttggtggttt ccatccagat tttacacaca ctggttaatg gcaccgaaag tagcatccct
    14701 accgggccga gcagccatcc ccaaattaac aatgaaagaa ataccaccat ggtggacatc
    14761 cccaggcgat ggcccatcat ccgtggttct aaaatattgc cgatgaccat atggaccact
    14821 aaaaacaatg cgccgaccag aatacattcg taaacaccat taaacagcag cacctgaatc
    14881 attggcggta cggcggaaat taccgcgccg atattgggca cgtagttgag caaaaacgcc
    14941 agtactgccc acatcagcgc aaactgcacc cccatcagct ccagccccag ccagacgatg
    15001 acacctgtcc ataaactgag tagcgtcttc aatgcaagat agtgcgaaac gcctttaagt
    15061 gcgcggtgta atcctgcgat gtgaatctgt gggttattca gcgcaaaacg cattttgtaa
    15121 gggacgtggc gcacttcaaa cagcataaaa actacggtca tcaccagcaa aagcacgctc
    15181 gccattgccc cggaaagccc ggtcattagc gctgtggtga aggtaaccac tttttccgag
    15241 tccatccgct gcagcattcg ctccggcgac atatgcaaat taagaaaagg caacatctcc
    15301 tgcaatttaa aaagtttgcg cgtcagctcc ttattaaact tcggcagcat agagataaat
    15361 tcgttaaacg atgccgccag tacgccgacc agcgcggtta gtgcgatcag catcaccacc
    15421 actacaatcg taatggcaac ggggcgttgt actccccgac gaataaacca ggtaacgagc
    15481 gggttgagga cgatggcaaa aaacagcgcc agtaacagct gcacaatgat atctgccgct
    15541 gcgtgaatgc ccgcgaggat cactaccagc gaggccagct tgagcagaat gtgcatgccc
    15601 gttttatcgg gttgagggat ttccattggg gcttccttgt gactttttgt attaagtgta
    15661 gcgggagtca cgccagcaat attctgattc ttactgctga ttttcacgtc agcacgtggt
    15721 aaaaatgaaa cactgttgta aaaatgtggt gatcctcatg cccgaacccg tagccgaacc
    15781 cgcgctaaac ggattgcgcc tgaatttgcg cattgtctcc attgtcatgt ttaacttcgc
    15841 cagctacctc accatcgggt tgccgctcgc tgtattaccg ggctatgtcc atgatgtgat
    15901 gggctttagc gctttctggg caggattggt tatcagcctg caatatttcg ccaccttgct
    15961 gagccgtcct catgccggac gttacgccga tttgctgggc cccaaaaaga ttgtcgtctt
    16021 cggtttatgc ggctgctttt tgagcggtct gggatatctg acggcaggat taaccgccag
    16081 tctgcccgtc atcagcctgt tattactttg cctgggacgc gtgatccttg ggattgggca
    16141 aagttttgcc ggaacgggat cgaccctgtg gggcgttggc gtggttggct cgctgcatat
    16201 cgggcgagtg atttcgtgga acggcattgt caattacggg gcgatggcga tgggtgcgcc
    16261 gttaggcgtc gtgttttatc actggggcgg cttgcaggcg ttagcgttaa tcattatggg
    16321 cgtggcgctg gtggccattt tgttggcgat cccgcgtccg acggtaaaag ccagtaaagg
    16381 caaaccactg ccgtttcgcg cggtgcttgg gcgcgtctgg ctgtacggta tggcactggc
    16441 actggcttct gccggatttg gcgtcatcgc cacctttatc acgctgtttt atgacgctaa
    16501 aggttgggac ggtgcggctt tcgcgctgac gctgtttagc tgtgcgtttg tcggtacgcg
    16561 tttgttattc cctaacggca ttaaccgtat cggcggctta aacgtggcga tgatttgctt
    16621 tagcgttgag ataatcggcc tgctactggt tggcgtggcg actatgccgt ggatggcgaa
    16681 agtcggcgtc ttactggcgg gggcagggtt ttcgctggtg ttcccggcat tgggcgtagt
    16741 ggcggtaaaa gcggttccgc agcaaaatca gggggcggcg ctggcaactt acaccgtatt
    16801 tatggattta tcgcttggcg tgaccggacc actggctggg ctggtgatga gctgggcggg
    16861 cgtaccggtg atttatctgg cggcggcggg actggtcgca atcgcgttat tactgacgtg
    16921 gcgattaaaa aaacggcctc cggaacacgt ccctgaggcc gcctcatcat cttaaaactt
    16981 actgaataac cagcgtatta atgatgtttt ctgcggtggt ctgcgctttt tgctgatcgt
    17041 cagctggcag agtaatttgc atggtcagca gttgattacc cacgttaccc agaataacgg
    17101 aagagtacgc cgtctggcct ttcgcggaga taatactgtc taactgctgc attttgtgac
    17161 ctttcagctc aatggctttg ttggttacca cttgcagctg cggatcgcgg ctacgttgct
    17221 gatcttccag acgcttcgcc aacaccgcca gatcttcttt cggatcatcg cccatgatga
    17281 cgatgactgc tttctgcccg gtggcgtcgg accagacatg catgttattg gcctgcgttc
    17341 ccagcttacc gctctggtcg gtcatatccg ctggcagcga gaaacttaac ttgccatcaa
    17401 gcaggttgac gggattcccg gtagcgttac tttccgcgac cgaaccctgc gccgtagcgt
    17461 tagtgtcttt atcatcacag gccgcaagcc ccataaccag caggccaatt ccgacatatt
    17521 taaccagatt gcgcattgac ttcttccttt cgataaacgg ccataacggc tcattcatcc
    17581 atcttatcac aactctgata acgaaccttt aactcgcctg caaagcgttg atttcggatt
    17641 tatctgccag tcttttcaac agaatattga gtaatacgcc atacattggc aggaagaaaa
    17701 cgatactgat taacactttg aaacagtaat cgaccagcgc gatttccatc cagtgttcag
    17761 ccataaaggc atccgggcta cgccagaagg caatgaagaa aaaggccagc gtgtcgctga
    17821 cgttaccgaa cagtgtggac gctgtcggtg ccagccacca gcggcgactc tgacgcaggc
    17881 ggttaaaaac gtgcacgtcg aggatctgtc ccagcgcgta ggccatgaaa ctggcggtgg
    17941 cgatacgggc gacaaacagg ttgaagtggg cgagtgcgcc gaatccctgc caggaaccca
    18001 tatagaatag cgacgagatg acgtaggaga tcagcaacgc cgggatcatc accgcaaaga
    18061 tgatacgacg ggccagcggt gcgccaaaaa tacgcacggt caggtcggta gcaaggaaaa
    18121 taaacgggaa gctaaacgcg ccccaggtgg tatggaaacc caaaatggag acgggaagct
    18181 gaaccagata gttactggag gtgatcacca gcagatgaaa taacgataac cagaacaacg
    18241 ccttatagcg ttgagtttgc gagaaaacgt tcatattgta cctttttgat taaccattgg
    18301 ggtgagggaa cccaatacgt acgacacgtc tgttacctta acataataac gtgcttaccc
    18361 ttttttcgag ccgccgcatg atactgcttt acgttgacaa tgcaatggtt aattttcacg
    18421 caatcgttaa cctggtttgc ttacggactc acagggcgta aactagcgcc gtttttttat
    18481 gtgatgagaa gaaaatgacc gatctctttt ccagccctga ccacacactc gacgcacttg
    18541 gcctgcgctg cccggaaccc gtaatgatgg tgcgcaaaac tgtgcgcaat atgcagcctg
    18601 gcgaaacgtt gctgattatc gccgacgatc cggccactac ccgcgatatt cctgggtttt
    18661 gtacctttat ggaacacgaa ctggttgcga aagagacgga tggactgcct tatcgttatt
    18721 tgattcgtaa aggcggttga tggggaaaga ttggcttcga tgccgccttt tcccctcacc
    18781 ctaaccctct ccccagaggg gcgaggggac cgatcgcgct caatgttgcg atcggtttgc
    18841 cttatctcct gcgcaacaat cttaacgcat tcgctgtcac cagcaccgtc gcacccgtat
    18901 ctgccagcac cgccagccac aggccggtca tccctaacag cgtggtgacg aggaagatcc
    18961 ctttcagccc cagcgcaatg gtgatgttct ggcggatatt ggcgtgagtg gcgcgtgcca
    19021 gttcaatcat ttgcaccagg ccgcgcaggt ggttatgggt taatgctgca tcagcggttt
    19081 ccagcgccac gtctgtgcca ctgcccattg caatcccaat ggcggcagct ttcatcgctg
    19141 gcgcgtcgtt aataccatca ccgaccatcg ccaggggcgc atgttgattc agcttggtca
    19201 ccgctttgac tttatcttcc ggcaataggc ccgctttaaa ctccagcccc agctccccgg
    19261 cgattgccgc cgctgcacgt ggattatcgc cggtgaggat cactcctttg accccc
//
LOCUS       DAZOYL010000051        19189 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZOYL010000051 DAZOYL010000000
VERSION     DAZOYL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.051"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(167..1816)
                     /gene="pgi"
                     /locus_tag="ACHUOY_001321"
     CDS             complement(167..1816)
                     /gene="pgi"
                     /locus_tag="ACHUOY_001321"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR0682148.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2053..2249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2341..3690
                     /gene="lysC"
                     /locus_tag="ACHUOY_001322"
     CDS             2341..3690
                     /gene="lysC"
                     /locus_tag="ACHUOY_001322"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR0682149.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3942..4214
                     /locus_tag="ACHUOY_001323"
     CDS             3942..4214
                     /locus_tag="ACHUOY_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR0682150.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4347..5219)
                     /gene="rluF"
                     /locus_tag="ACHUOY_001324"
     CDS             complement(4347..5219)
                     /gene="rluF"
                     /locus_tag="ACHUOY_001324"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR0682151.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5567..6404
                     /locus_tag="ACHUOY_001325"
                     /pseudo
     CDS             5567..6404
                     /locus_tag="ACHUOY_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            6474..7280
                     /locus_tag="ACHUOY_001326"
     CDS             6474..7280
                     /locus_tag="ACHUOY_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0682152.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7290..7697
                     /locus_tag="ACHUOY_001327"
     CDS             7290..7697
                     /locus_tag="ACHUOY_001327"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR0682153.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7697..8191
                     /locus_tag="ACHUOY_001328"
                     /pseudo
     CDS             7697..8191
                     /locus_tag="ACHUOY_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            8257..9054
                     /locus_tag="ACHUOY_001329"
     CDS             8257..9054
                     /locus_tag="ACHUOY_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR0682154.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9065..9889
                     /locus_tag="ACHUOY_001330"
     CDS             9065..9889
                     /locus_tag="ACHUOY_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR0682155.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9941..11169
                     /gene="sorE"
                     /locus_tag="ACHUOY_001331"
                     /pseudo
     CDS             9941..11169
                     /gene="sorE"
                     /locus_tag="ACHUOY_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            11552..12242
                     /gene="pepE"
                     /locus_tag="ACHUOY_001332"
                     /pseudo
     CDS             11552..12242
                     /gene="pepE"
                     /locus_tag="ACHUOY_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
     gene            complement(12333..13964)
                     /locus_tag="ACHUOY_001333"
     CDS             complement(12333..13964)
                     /locus_tag="ACHUOY_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR0682156.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14184..17867)
                     /gene="metH"
                     /locus_tag="ACHUOY_001334"
     CDS             complement(14184..17867)
                     /gene="metH"
                     /locus_tag="ACHUOY_001334"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR0682157.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18067..18891
                     /gene="iclR"
                     /locus_tag="ACHUOY_001335"
     CDS             18067..18891
                     /gene="iclR"
                     /locus_tag="ACHUOY_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR0682158.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4525 a         5011 c         5016 g         4637 t
ORIGIN      
        1 ggatttatct gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac
       61 tcctcatact atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt
      121 aaacgcctta tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg
      181 ctttatagcg gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttgtcat
      241 ctttcagctc tggcagaata cggttcgcca gttgtttacc caactccacg ccccactggt
      301 cgaaggtaaa gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa
      361 tcaacgcacc caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt
      421 taccttcgaa cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat
      481 cgcgatattc ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg
      541 cgaagaagtt agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg
      601 gggcgatgaa atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt
      661 gctgaccgtt agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt
      721 taccgttacg gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg
      781 cgaaacggtg catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat
      841 tgttgtacca gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg
      901 tggagaaatg cttgtccatc gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc
      961 cgatggagag aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc
     1021 agtcccagaa ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat
     1081 tggtggaaag cgccgcaaag tgttttgcaa cgtgtttttc atcacctgcc gctttcagga
     1141 accagtcacg cgcgctatgg gcgttggtca tagtttcctg agtggtgaag gttttagatg
     1201 ctaccaggaa cagcgtggtt tccgggttta cttttttcag cacttcagcg atgtgagtcc
     1261 catcgacgtt agaaacaaag tgcatgttca ggtgattttt gtacggacgc agtgcttcgg
     1321 tcaccatgta cgggccgagg tcagaaccac cgatcccgat gttcactacg tcagtgattg
     1381 ctttgccggt ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct
     1441 tctccagcac cgcgttgact tccggcatta cgtctttgcc atcaaccaga atcggggtat
     1501 tgctacggtt acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct
     1561 cgccagagaa catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta
     1621 atttcgccag cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt
     1681 cgaatgttgc ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg
     1741 taacgtcttt catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg
     1801 gattgatgtt tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt
     1861 gtagcgccag tcacagaaaa atgtgatggt tttagtgccg ttagcgtaat gttgagtgta
     1921 aacccttagc gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa
     1981 aatccgcatg accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta
     2041 cctgcgctag cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag
     2101 tcctgtgata acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca
     2161 tcatcggcta caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt
     2221 ttcgcaagtg gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac
     2281 ccgtcttccg ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt
     2341 atgtctgaaa ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg
     2401 aaccgcagcg ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct
     2461 tctgctggta tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga
     2521 ttcgaaaaac tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac
     2581 ccgaacgtta tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa
     2641 gcggcggcgc tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg
     2701 atgtcgaccc tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt
     2761 gatgtacgta aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc
     2821 gcgctggcgg aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc
     2881 acccagggat ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc
     2941 agcgattata cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg
     3001 accgacgtcc cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt
     3061 gatgaaatcg cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat
     3121 ccggcaacgt tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa
     3181 gacccacgcg caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc
     3241 gcgctggcgc ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat
     3301 tctcgcggtt tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac
     3361 ttaatcacca cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc
     3421 actggcgata cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg
     3481 gaagtagaag aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc
     3541 ggcgttggca aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac
     3601 ggcgcatcca gccataacct gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg
     3661 caaaaactgc atagtaattt gtttgagtaa atactgtatg gcctggaagc tatatttcgg
     3721 gccgtattga ttttcttgtc actatgctta tcaataaacg agcctgtact ctgttaacca
     3781 acgtctttat cgagaataat tgcctttaat ttttttatct gcatctctaa ttaattatcg
     3841 aaagagataa atagttaaga gaaggcaaaa tgaatattat cagttctgct cgcaaacgaa
     3901 ttcccgtaca ctgaactctc tggctcactg agggcaccat catggcactc ccccgtatta
     3961 cccaaaaaga gatgactgaa cgcgagcagc gtgaacttaa aacactgctg gatcgggcgc
     4021 ggattgcgca tggtcgcgta ctgaccaact ccgaaaccaa cagcattaag aaagagtaca
     4081 tcgataaatt aatggtcgag cgtgaggctg aagcgaaaaa agcccgccaa ttgaagaaaa
     4141 agcaggctta taaaccggat cctgaagcgt catttagctg gtcagcaaat acgtcgacgc
     4201 gcggcaggcg ctaatgaaca atcaacgcct gagattttca ctcgtcagtg tgaacacaat
     4261 tttcccctca ccctaaccct tttcccgaag gggcgagggg actgtccgag cacttttggg
     4321 ccgttttcat cagtctgagg cgttaatcag cgccccttct ttttacgccc cggcgaggta
     4381 aaacgcttac cgttggacgc cgggcgaccg cctttttccg ccgttttttc catcttaacg
     4441 actggacgtt tgatgcccgc tgttttcggt ttggccttcg ctttgggctt cgcctcggaa
     4501 gaggaatttt caatgagctt aaagagatcg ataagctcgt cgtcggttaa atcgcgccat
     4561 tctcccagcg gaatgccgct taagctgacg ttcataatgc gcgtacgttc cagctttttc
     4621 acttcatagc cgaaatgctc gcacatgcgc cggatctgac ggttcaaccc ctgcaccagg
     4681 gtaatgcgga agacaaacgg tgcttctttt ttgactttgc actttttcgt caccgtcccg
     4741 aggatcggca ctcccgcccc cataccacga ataaattcat cggtaatcgg tttatcgacc
     4801 gtcaccagat actctttctc atgatcgtta cccgcacgga ggatcttatt caccagatcg
     4861 ccgtgattgg tgaggaaaat cagcccctgg gagtctttat ccaggcggcc aatcgggaac
     4921 acgcgtttgc tgtggttaac gaaatcgaca atgttatcgc gctcgccatc ttcggtggtg
     4981 ctgacaatac cgacgggctt gttcagggcg ataagtacca aatcttcggc ttcccgaggc
     5041 tcaatcaact gaccgtttac tttcacaatg tcgccgggtt tcacctgatc accaatggtg
     5101 gctcgcttgc cattaaggaa cacattgcct tgctcgatat agcgatccgc ttcgcggcgt
     5161 gagcaaattc cgctttcgct gatgtattta tttaaacgga ctgatgagtc gggcagcata
     5221 aattctcctg taaaacgcgg aatataccgt acctcttagc ggggaaaaac agggttatgc
     5281 ggcaacgtcg aaattaaatg tgatctgcca cgccttttat cacaaaagtg tctccggaag
     5341 tacgatgcaa atttgcctgg caaaataagg ataatcaaca tattacgaat cgctgcacac
     5401 ctgtgctgat gactactacg cagagaaaca aatgtgcaag agaaagcgct atttctcccc
     5461 atgcaaagag tgaaaaatcg ctttgaaagg ccctcataat ggggttatat cagatcaagc
     5521 ggtgagaaag cgtatttgca caaatgtgca gaaccaggag gcggcaatgg aaaacagtga
     5581 cgatatccgt ttgattgtga agattgccca actctattac gaacaggata tgacgcaggc
     5641 gcaaatcgcg cgcgaactgg gtatttaccg caccaccatc agccgcttgc ttaaacgagg
     5701 ccgcgatcag ggaattgtca ccatcgccat caactatgac tacaacgaaa atctctggct
     5761 ggagcagcaa ctgaagcaaa agtttggctt gaaagacgtt gtggtggtgt cgggaaatga
     5821 tgaggatgaa gagactcaac tggcgatgat ggggttacac ggcgcgcaac tgctggatcg
     5881 cttgctggag cctggcgata ttgtcggttt ttcctagggt cgcgcggtga gcgcactggt
     5941 tgaaaacttg ccgcaagcgg ggcaatcgcg gcagttaatc tgcgtgccaa ttattggcgg
     6001 accgtccggt aaactcgaaa gccgctatca cgtaaacaca ttaacctaca gcgtggcagc
     6061 gaagctgaaa ggggaatcgc atctcgcgga ttttccggct ctgctggata accccttaat
     6121 tcgtaatggg atcatgcagt ctcagcactt taaaaccatc tctgcctact gggataatct
     6181 ggatgtcgcc ctggtgggaa ttggctcacc ggccattcgc gacggcgcta actggcatgc
     6241 gttttatggt ggtgaagaga gtgacgacct gaaagcaggc gcggtattcc attggcattg
     6301 ccatgagtga agaaaaatac agcggaattg ttggtgcact gcgtggaaaa tatattaatt
     6361 gtctggtaac gaatagcagc acagctgaac tattactgaa ataaaaatct tcccgatgtt
     6421 taccgtaata aaaattacgg cgggtcccta ttatctaaaa tcaggagtct gttatgcaaa
     6481 cgtggttaaa tttgcaggat aaaatcatta tcgtcaccgg cggcgcatcc ggtattggtc
     6541 tggcgattgt agaggaatta ttagcacaag gcgcaaacgt acagatggta gatattcacg
     6601 gtggcgatgg tcaatatgaa gttcataaag gctatcagtt ttggccgacc gatatttcca
     6661 gcgccaaaga ggtaaatcat acggtagcgg aaattatcca gcgttttggt cgcatcgacg
     6721 gtctggtcaa taacgccggg gtcaatttcc cgcgtctgct ggtcgatgag aaagcgcctg
     6781 ccgggcagta tgaactcaac gaagctgcat tcgaaaaaat ggtcaatatc aaccaaaaag
     6841 gcgtttttct gatgtcgcag gcggtggcgc gccagatggt caaacaacat gatggcgtga
     6901 ttgtgaatgt ttcctcagaa agtggactgg aaggctcaga aggccaaagc tgttacgccg
     6961 ctaccaaagc cgcgctcaat agcttcacgc gctcctggtc gaaagagctg ggtaaacacg
     7021 gtatccgtgt ggtcggtatc gcgccgggga ttctggaaaa aaccgggcta cgcacgccgg
     7081 aatatgaaga ggcgctggcg tggacgcgca atatcaccgt cgagcagctg cgtgaaggct
     7141 ataccaaaaa cgccattcct attgggcgcg ccggaagatt agcagaagtc gctgattttg
     7201 tttgttatct gctgtctgaa cgcgccagct acatcaccgg agtaaccact aacattgcgg
     7261 gcggcaaaac gcgcggataa ggaggcttta tggtcaacgc cattttttgt gcccacggca
     7321 aattagcttg cgccatgctg gaatcggttc agatggtgta cggcgacgcc aatgtcgagg
     7381 cggttgaatt tgtaccaggc gagaacgccg gagacatcgt agcaaagctg gaaaagttag
     7441 taagcattca caatcaagat gagtggctga ttgccgtgga tttacagtgc ggcagcccgt
     7501 ggaatgcggc agcgatgctt gcgatgcgca atcctcgtct acgggtaatc agcggccttt
     7561 ccctgccgct ggcgctcgaa ctggttgata accaggacag catgaatgtt gatgaactgt
     7621 gtgaacacct gacgcaaatc gcaaagcaaa cctgcgttgt ctggaaacaa ctggcaacgg
     7681 cagaggaaga tttctgatga acattacgct cgcccgcatt gatgaccgtc tgatccacgg
     7741 ccaggtcact accgtctggt cgaaagtggc taacgcccag cgcattatta tctgcaatga
     7801 cgaagtttat aacgatgaag tgcgccggac attattgcgt caggctgcgc caccgggaat
     7861 gaaggttaac gtggtcaata ttgaaaaagc cgtcgcggtt tatcacaacc cgcaatatta
     7921 ggacgaaacc gttttttatc ttttcactcg cccacaggat gccttagcga tggttcgcca
     7981 gggggtaaaa ataggcaccc tgaatattgg cggcatggcc tggcggcccg gtaaaaaaca
     8041 gttaaccaaa gcggtttcat tagatgatga tgatattaac gcatttcatg agttaaataa
     8101 tcttggcgtc ataatggatt tacgcgtcgt agcgtcagat ccctcaatca atattatcga
     8161 caaaataaac gaacagttaa tcgcaaatta aataatagcg cctgtgtaca tatgctttaa
     8221 caggcaggga tgacgacact taaaaggtga caaatcatgg aaataagtac cctacaaatt
     8281 attgctatat ttcttttttc ctgtattgcc ggaatgggca gcgtgctgga tgaatttcag
     8341 actcatcgcc cgttaattgc ctgtacggtg attggtttaa ttctcggtga tttaaaaacc
     8401 ggaataatac tcggtggtac gctggaattg atagctctcg gctggatgaa cgtcggcgcg
     8461 gcgcaatctc cggattctgc actcgccagc ataatctccg ccattctggt tatcgttggt
     8521 cagcaaagca tcgccaccgg aatcgccatc gcgttgcctg tggctgcggc aggccaagtg
     8581 ctgacggtgt ttgcccgtac catcaccgta gtgttccagc acgcggcgga taaagcagcg
     8641 gaagaggcgc ggtttcgcac tctcgatatt ctgcatgtct ccgcgcttgg cgtgcaggcg
     8701 ctgcgcgttg ctattccggc actgattgtc tcactgttcg tcagcgccga tatggtcagc
     8761 aatatgttaa acgccattcc cgaatttgtg acccgtggac tgcaaattgc tggcggtttt
     8821 atcgtggtgg tcggttacgc catggtgcta cgtatgatgg gcgtgaaata tttgatgcct
     8881 ttctttttcc tcggtttcct cgcaggtggc tacctcgatc tcagcctgct ggcgttcggt
     8941 ggcgtcggcg tgatcatggc cctggtctac atccagctaa atccacagtg gcgtaaagct
     9001 gaacctcaac tccaggccac cgcctccacc gcccttgacc aacttgacga ttaacggagc
     9061 catcatggaa cagagaaaaa ttacacgcag cgatctggtg agcatgtttc tgcgctccaa
     9121 cctgcaacag gcgtccttta actttgaacg tattcacgga ctgggctttt gctacgacat
     9181 gatccccgcc atcaagcgac tttacccatt aaaagaggat caggttgcgg cgctcaggcg
     9241 acacctggtg ttcttcaata ccacgccagc cgtatgtggc ccggtcatcg gcgtcactgc
     9301 cgccatggaa gaggcgcggg ccaacggcgc ggaaattgat gacggtacca ttaacggcat
     9361 caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct ggggaacgct
     9421 gcgcccgatt accgctgcgc tcggcgcatc tctggcactt tctggcaaca tcctcggccc
     9481 gctgctgttt ttctttattt tcaatgcggt gcgtctggcg atgaagtggt atggcctaca
     9541 gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcgggaatg tgctgcaaaa
     9601 actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg ggcgtgctgg tcaccaaatg
     9661 gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat gccgccgatg gctccaccgt
     9721 catcatgacc gtgcagaaca ttctcgacca actttgccct ggtttgctgg cgctcggtct
     9781 gacgctactg atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc
     9841 cctgtttggc ttagggatta tcggcaatgc gctgggcttc ctgtcctgat tcttcgcccc
     9901 ggcacgactg ccggggccat cgctcaacat gaggtggttt atgaaaacaa cagctctgcg
     9961 tctttatggt aaacgtgatt tacgcctgga aacctttgac cttcctgaaa tgcaggagga
    10021 tgaaatcctc gcgacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggcaac
    10081 ctgggtgaaa accataaaaa agtacccgac gatgtggcga ccaacccaat catcatcggc
    10141 cacgagtttt gcggcgatat tctggccgtg ggtaaaaagt ggcagcacaa attccagccg
    10201 ggtcagcgtt atgtgattca ggccaacctg aaactccctg accgcccgga ctgccccggc
    10261 tactccttcc cgtgggtggg cggcgaagcc acgcatgtgg ttattcccaa cgaggtcatg
    10321 gaacaagatt gcctgctggc atacgacggc gaaacctatt ttgaaggctc gctggttgaa
    10381 ccgctttcct gcgtgattgg cgcgttcaac gccaactatc atcttcagga aggtagttat
    10441 aaccacacga tggggattcg cccgcaaggg cgcacgctga tcctcggcgg caccggacca
    10501 atgggattgt tggcgattga ttatgcgctg catggaccag ttaacccgtc gctgctcgtc
    10561 attaccgata ccgacaacga caaattgagt tatgcgcgca agcactatcc atcagaaccg
    10621 caaacactga ttcattatct caatgccacc gatgcagcat ttgatacgtt aatggcgctg
    10681 agtggcggtc acggcttcga tgatattttc gtctttgtgc ctaatgaagg actggtgact
    10741 ctcgcctctt ccttgctggc gacagatggt tgcctgaatt tcttcgccgg accgcaggat
    10801 aaacatttca gcgcgccaat taatttctac gatgtgcatt atgcatttac ccactacgtg
    10861 ggcacgtcag gcggcaatac cgacgacatg cgcgcggcgg tcaaactgat tgaagagaaa
    10921 aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc tgaatgccgc gggcgaaacc
    10981 acgcttgaat tgcctgccgt cggcggcggc aaaaagctgg tgtataccgg gaaatacctg
    11041 ccgctgacgt cactcacgca gattcaggat caagcactgg cggcgattct ggcgcgtcat
    11101 caggggatct ggtccggtga ggcggagcaa tatctgctca ctcatgcaga ggcaatttcc
    11161 catgattaat cgcgctactt aactctgcat gtcgctggct ggtcgccgcg gcaacttcgc
    11221 gacctgatgt gcggctgatt gctgaagcgg cccaatgtgc acgagaagga taaaaaaaca
    11281 tcagcgcctt aaggggcgct caaattggtg gcaaagtaca cattgtttat gccggatgcg
    11341 gaataaagcc ttattcggcc tacataatct cgaaaattca atatattgca gttatctggt
    11401 aggcctggta agcgtagcgc atcaggcaat gctgtgtttg tcatcagtct cagcgcccgg
    11461 tgcaatacgt gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcatgc
    11521 cttccgtgat ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
    11581 gctgccgggt aaagcctggc tggaacatgc actgccgcta attgctgaac agttgcaggg
    11641 tcgccgctca gcggtgttta tccctttcgc tggcgtaacg cagacctgtg atgattacac
    11701 agcgaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
    11761 tgtcgatccc gttgccgcga ttgaaaatgc tgagatcgtg attgtcgggg ggcgggaata
    11821 ctttccagtt gctgaaacag tgccgcgagc gcgggctgct ggcaccaatt actgacgtgg
    11881 ttaaacgtgg tgcgctgtat attggctgga gcgcaggcgc taaccttgct tgcccaacca
    11941 ttcgtaccac caacgatatg ccgattgtcg atccgcaagg tttcgatgcg ctaaatctgt
    12001 tcccgctgca aatcaacccg cacttcacca acgcgctgcc ggaaggccat aaaggtgaaa
    12061 cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc agaactgacg attattggtc
    12121 taccggaagg taactggatc acggtgagta aaggtcacgc tacgctgggt ggcccgaaca
    12181 ccacttatgt gtttaaggcc ggtgaagaag cggtgccgct ggaagcgggt caccgttttt
    12241 aagtgaattg ccggatgcat gatccggcat cttgttcata gtgcctgatg cgacgcttga
    12301 cgcgtcttat caggcctaca ggattcacaa tattaatact cgtcccgtcc ttcatcttca
    12361 tccggctgtt ccagcacact gtaagccacc gaacaaaaca gcgagttcag acgctgcata
    12421 tctcccagta agcctaaatg tagcgaactg gtttcaatgc tctgcacgtt ttgctgatgc
    12481 aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa tgcgaaagcg atgcttgctg
    12541 cggcgcaaac gacgagcgct ggtgacatcg ccagagaaga acaccgacat tgccagcttt
    12601 aaattactga gcaattgctc atagagcgca tccagttcct tcaacccatc aagcgaaaat
    12661 gcccgccgtg ctgccagcga tttatcagca atttcgctgc ccatgcgctc gacgatatcg
    12721 gaagcctgtt caaggttgag cgacatttca ataatctccg cccagcgacg cgactcttct
    12781 tctgccagtt cctcttttgg cattcgcgcc agatacagct taatggcggt gtagagaacg
    12841 ttgatatcat ctgccagctt acgcagctct ttctcctgcc gtggctcgcc gtgcatcact
    12901 ttattcagcc cttccatcat ctgttccatg gcgtcgccaa tgcgcagggt ttcgcgcgcg
    12961 gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga catccagatg tttaggccgt
    13021 agctgggtat ccagttccgg ttcatcgcga ataatcgttt tgcaaaaccg tgccatcggg
    13081 tcaacaaatg gcagcatgac caggcaacgt acaaggttgt agaagacgtg gaaatagatc
    13141 accagttccg cttttggcaa tgacaacttc cccattgtct ctgccagcaa atggacaaac
    13201 ggcaggataa tcaggctacc caccagctta aacagcaaac tacccagcgc gacacggcgg
    13261 gctgcggcat tggcggcact gttgttgagc atcgccagca ggccggaacc gaggttagca
    13321 ccaatcacca gacagagcgc cacggggaag gagataatgc ctgcggcggt cagagtcgcg
    13381 gtcagcagta cagcagcaag gctggagtag ctgataatgg cgaacatcgc gccaatcagc
    13441 gcatccagca gaatatcgcc ggtcagcgag gcaaagatca cctgaacgcc gtttgcctgg
    13501 gtgatcggcg ttacggcctg cacaatcaac tccagcgcca gcaaaatcag cccaagacca
    13561 ataccgacgc ggcccagttg cccggcgcgt gactgtttgc gtccgaggaa aaaaatcacg
    13621 ccgataaaaa taagtaacgg tgacagccag gataagtcga aggtgagaat acgcgccatt
    13681 agcgccgtcc cgacatctgc acccagcaca atcaccagag ccggtgcgag ggctaccaga
    13741 tcctgagcga cgaacgaggt caccagcatg gtggtggcat tactgctctg taccagtgcg
    13801 gtaacgccga tccccgcgca aaaggcgagc ggcttttttt cgacgctccg gctaaggaca
    13861 gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc gaacaatatg agtcccccag
    13921 accagcaggg cgacggcaga aagcaggtga agcagcgtta acacgagttc aggttctcct
    13981 tatcgttata tgttcctgaa gcaattgaat gaccaaagga ccggaaacac gctgactggc
    14041 atctttactg tgccttttga cattcgtttt tccggctaag attattccag cctgtttcca
    14101 gtataagtgt ttcatattaa taaagagaca gggcactcat tgagcgccct gtttgttgta
    14161 aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc ccagattcgg tgccagccag
    14221 cgctcaacat cggagacgct cataccttta cggcgggcat agtcttcaac ctgatcgcgc
    14281 tgaatttgcg ccaccgcgta gtacttgctg tccgggtggc tgaaatacca acccgaaacc
    14341 gatgcaccag gccacatggc gaaagattct gtgagtttca tgccagtatg tttttccact
    14401 tccagcagct cccagatggt ggctttttcc gtatgttccg ggcaggccgg gtagcccggt
    14461 gccggacgga tgccctggta gttttcgcgg atcagttctt cgttgctgag gttctcgttc
    14521 ggcgcatagc cccagtagac tttacgcaca cgctcatgga gatactccgc aaaggcttcg
    14581 gctaaacggt cggcaagcgc tttcaccatg attttgttgt aatcatcgtg ctgcgcttca
    14641 aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca cggcaaatgc gccgatgtaa
    14701 tctgctttac cagaaagctt cggcgcaacg aagtcagcga gacagtagtt agcgaatccc
    14761 gttttttcgg tctgttgacg cagatggtgg ctgacgttga tcacatgggt gcgcgtttcg
    14821 tcacggtaga tttcaatgtc atcgcccaca cggtttgccg ggaacaagcc caccacgcca
    14881 cgcggattca gcgttttctc ggcgcttaat ttatccagca tgtcgttggc gtctttaaac
    14941 agccgctgcg cctcaacgcc caccacttca tcttccagaa tgcgcggata cttcccggcc
    15001 agcgaccagg tcataaagaa cggtgtccag tcgatgtaat tacgcagagt ttcgatgctg
    15061 gcttcaactt cctgcacgcc gagacggtgc gccaccggcg gcgtgtaagc ctgccagtca
    15121 aaagcgaagt cgttatcgcg cgccgcttcc agcgtgaccg gtggtgtgcg cggtttcttg
    15181 cgcccgtgct gaatacgtac ggtttcatac tccttgcggg tacgagcgac aaaatcatcg
    15241 cgctgagtat cggaaagcag cgccgccacc acaccaacgg tgcgcgaggc attctgcaca
    15301 tacaccgtcg ggccgctgta gttctgctca attttcaccg ccgtgtgcgc ttttgaggtc
    15361 gtcgcgccac cgatcagtaa cggaatggtg aagccctgac gctccatctc tttcgccacg
    15421 ttaaccatct cgtccagcga cggcgtgata agccccgaaa gtccaatcag atcagcattc
    15481 acttctttag cggtacggag aattttttcc gcaggcacca taacgccgag atcgacaatt
    15541 tcgtagttgt tacattgcag caccacacca acgatatttt taccgatgtc gtggacgtcg
    15601 cccttcacgg tggcgatcac catcttgccg ttggttttgc cctgctcttt gctggcttca
    15661 ataaacggtt cgaggtaggc cactgcctgt ttcatgacgc gcgccgattt gaccacctgt
    15721 ggcaggaaca ttttcccctc gccaaacaga tcgccgacca cgttcatgcc gtccatcaac
    15781 gggccttcaa tcacttcaat cgggcgcgta gcctgctggc gggcttcttc ggtatcctgc
    15841 tcgataaact cagtaatgcc tttgaccagc gagtattcca gacgtttatt cacttcccac
    15901 gagcgccact ccgcctgctg ggcgttagcg gtgtcgtcgg ttttgctgcc gcgatatttc
    15961 tcggcaagct ccagtaaacg ttcagtgcca tcatcgcgac gattaagaat cacatcttcc
    16021 accgcgtcgc gcagttcagc gggcaggtcg tcgtaaatcg ccagttgccc ggcgttgacg
    16081 atccccatat ccatgccatt gcgaatagcg tagtagagga acaccgcgtg aatggcttcg
    16141 cgcaccggtt cgttgccacg gaatgagaaa gaaacgttag atacgccgcc ggaaatcagc
    16201 gcgtgcggca gttcgcgttt gatgtcttcg caggcgccga taaagtcctg cgcatagttg
    16261 ttgtgctctt caatgccggt ggcgaccgcg aagatgttcg ggtcgaagat gatatcttct
    16321 ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc gacggcaaat ctcgattttc
    16381 cgtgcgcggg tatcggcctg tccctgttcg tcaaaggcca ttaccaccac cgctgcaccg
    16441 taacgacgca acagtttcgc atgatggata aaggcgtcga cgccctcttt cattgagata
    16501 gagttaacaa tgcctttgcc ctgaatacac ttcagcccct tttcgatgac ctcccatttt
    16561 gaggagtcga tcataatcgg cacgcgagcg atatccggtt caccggcaat cagattgaga
    16621 aaacgcacca tcgccgcttc ggcgtcgagc atcccttcat ccatattgat atcgataatc
    16681 tgcgcgccgt tttccacctg ctggcgggcc acatccagtg cctcgctgta tttctcttct
    16741 ttgatcaggc gcttgaactt agcggaaccg gtgacgttgg tgcgttcacc cacgttcaca
    16801 aacaggctat cttcgccaat gttcagcggc tccaggccgg acaaacggca ggctacgggg
    16861 atttccggca gtttgcgcgg cgctaatcct tctactgcac gactcatcgc tgcaatatgt
    16921 tgtggcgtgg tgccacagca gccgccgacg atattgagaa agcccgcttc cgcccattca
    16981 cgtatttgtt ttgccatcgt gtcggcgtcg agatcgtact caccaaaggc gttgggtagc
    17041 ccggcgttcg ggtgcgcggt gacgtagcat tccgcaatcc gtgacagctc ctgcacgtac
    17101 tggcgcagtt catcgggccc cagcgcacag ttcaggccaa aggtcagcgc ttcggcgtgg
    17161 cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga gcgtgcgccc ggaggcgtcg
    17221 gtgatggtgc cagagatcat aatcggcagc tcaacgccca gcgtttcaaa ctccgttttt
    17281 accgcaaata ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat caggatcaga
    17341 tccgcaccac cttccaccag cgctttggtg gactctcgat aagccaccac cagcccgtca
    17401 aaagtgatat tacgaaatgc cggatcgttg acgtccggag aaatagacgc cgtgcggttg
    17461 gttgggccga gaacaccggc aacgtagcgc ggtttctccg gcgtgcgcgc ggtccactcg
    17521 tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga tttccgccga cagggattcc
    17581 atctggtaat ccgccatcgc aatggtcgtg gagttgaagg tgttggtttc gatgatatcc
    17641 gcgcccgctt caaagtaggc gttgtggata gcggcgatca cttccggttt actgagtacc
    17701 agcaggtcgt tgttgccttt gaggtcgcat ggccagtcgg caaagcgttc accacgaaaa
    17761 tcggcttcgt tcagtcgata gctctggatc atggtgccca taccgccgtc cagcaccaga
    17821 atacgttcat ttaactgcgc acgcagttgt tccactttgc tgctcacact tgctcccgac
    17881 acgctcaacc cagatttaat aaaaattcaa caaaccatac tggcataaac gcatctgtgg
    17941 taaaagcgac caccacgcaa catgagattt gttcaacatt aactcatcgg atcagttcag
    18001 caactattgc attaactaac aataaaaatg aaaatgattt ccacgataca gaaaaaggag
    18061 actgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt tgccaccgca
    18121 ccagcgactg gacaggttca gtctttaacg cgtggcctga aattactgga gtggattgcc
    18181 gaatccaatg gcagtgtggc actcacggaa ctggcgcaac aagccgggtt acccaattcc
    18241 acgacccacc gcctgctaac cacgatgcaa cagcagggtt tcgtgcgtca ggttggcgaa
    18301 ctgggacact gggcaatcgg cgcacatgcc tttatggtcg gcagcagctt tctccagagc
    18361 cgtaatttgt tagcgattgt tcaccctatc ctgcgcaatc taatggaaga gtctggcgaa
    18421 acggtcaata tggcggtgct tgatcaaagc gatcacgaag cgattattat cgatcaggta
    18481 cagtgtactc acctgatgcg tatgtccgca cctatcggcg gtaaattgcc gatgcacgct
    18541 tccggtgcag gtaaagcctt tttagcccaa ctgagcgaag aacaggtgac gaagctgctg
    18601 caccgcaaag ggttacatgc ctatacccac gcaacgctgg tgtctcctgt gcatttaaaa
    18661 gaagatctcg cccaaacgcg caaacggggc tattcatttg acgatgagga acatgcactg
    18721 gggctacgtt gccttgcagc gtgtattttc gatgagcacc gtgaaccgtt tgccgcaatt
    18781 tctatttccg gaccgatttc acgtattacc gatgaccgcg tgaccgagtt tggcgcgatg
    18841 gtgattaaag cggcgaagga agtgacgctg gcgtacggtg gaatgcgctg actttttctg
    18901 gcgggcagag gcaatattct gcccatcata cctgagtggc aatagaataa gggtgtctgt
    18961 taatcgcatt gacgccaaaa taacttaatg tcatacactt cacacacttt tattaactca
    19021 gcattatttt taaacatcaa accacttaat tattaacaaa atcgcaagat ctcattccac
    19081 cgaaactttt acggctgcat tatgagattg agcgatttat gatatttctt tagttgtaat
    19141 catattaaat tttttcactg ttctgatagt taaaatttaa gacatcaat
//
LOCUS       DAZOYL010000052        19091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZOYL010000052 DAZOYL010000000
VERSION     DAZOYL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.052"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            80..2470
                     /gene="gcd"
                     /locus_tag="ACHUOY_001336"
     CDS             80..2470
                     /gene="gcd"
                     /locus_tag="ACHUOY_001336"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR0682159.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2517..4067)
                     /gene="cueO"
                     /locus_tag="ACHUOY_001337"
     CDS             complement(2517..4067)
                     /gene="cueO"
                     /locus_tag="ACHUOY_001337"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR0682160.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4233..4580
                     /locus_tag="ACHUOY_001338"
     CDS             4233..4580
                     /locus_tag="ACHUOY_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR0682161.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4686..5552
                     /gene="speE"
                     /locus_tag="ACHUOY_001339"
     CDS             4686..5552
                     /gene="speE"
                     /locus_tag="ACHUOY_001339"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR0682162.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            5568..6362
                     /gene="speD"
                     /locus_tag="ACHUOY_001340"
     CDS             5568..6362
                     /gene="speD"
                     /locus_tag="ACHUOY_001340"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR0682163.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(6400..6762)
                     /gene="yacL"
                     /locus_tag="ACHUOY_001341"
     CDS             complement(6400..6762)
                     /gene="yacL"
                     /locus_tag="ACHUOY_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR0682164.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(6937..9534)
                     /gene="acnB"
                     /locus_tag="ACHUOY_001342"
     CDS             complement(6937..9534)
                     /gene="acnB"
                     /locus_tag="ACHUOY_001342"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR0682165.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKIEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            9889..11646
                     /locus_tag="ACHUOY_001343"
     CDS             9889..11646
                     /locus_tag="ACHUOY_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR0682166.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(11717..13141)
                     /gene="lpdA"
                     /locus_tag="ACHUOY_001344"
     CDS             complement(11717..13141)
                     /gene="lpdA"
                     /locus_tag="ACHUOY_001344"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR0682167.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13466..15358)
                     /gene="aceF"
                     /locus_tag="ACHUOY_001345"
     CDS             complement(13466..15358)
                     /gene="aceF"
                     /locus_tag="ACHUOY_001345"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR0682168.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(15373..18036)
                     /gene="aceE"
                     /locus_tag="ACHUOY_001346"
     CDS             complement(15373..18036)
                     /gene="aceE"
                     /locus_tag="ACHUOY_001346"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR0682169.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18197..18961)
                     /gene="pdhR"
                     /locus_tag="ACHUOY_001347"
     CDS             complement(18197..18961)
                     /gene="pdhR"
                     /locus_tag="ACHUOY_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR0682170.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         4403 a         5288 c         4792 g         4608 t
ORIGIN      
        1 attgtgatga cggtcacaca tgttaaaacc tataattaat tgctactaaa atattaatga
       61 attgaaatgg tgtctcttta tggcaattaa caatacaggc tcgcgacgat tactcgtcac
      121 gctaacagcc ctttttgcag cgctttgcgg gctgtatcta ctcattggcg gaggctggct
      181 ggtcgcgatt ggcggctcct ggtactaccc tatcgctggc cttgtgatgc tcggcgtcgc
      241 ctggatgctg tggtgcagta aacgcgccgc gctttggcta tacgcagccc tgctgctcgg
      301 caccatgatt tggggcgtct gggaagttgg tttcgacttc tgggcgctga ctccgcgcag
      361 cgatattctg gtcttcttcg gcatctggct gatcctgccg tttgtctggc gtcgcctggt
      421 cattcctgcc agcggcgcag ttgccgcact ggtggtggca ctgctgatta gcggtagtat
      481 cctgacctgg gccggattta acgatccgca ggaaatcaac ggcaccttaa gcgccgatgc
      541 cacacctgcg gaagctatct cacccgttgc cgatcaggac tggcctgcct atggtcgcaa
      601 tcaggaaggt caacgctttt cacctctgaa acaaattaac gccgataacg tccataatct
      661 gaaagaagcc tgggtgttcc gtactggcga tgtgaagcag ccgaacgatc cgggcgaaat
      721 caccaatgaa gtgacgccga ttaaagtggg cgacaccctt tacctgtgta ccgctcacca
      781 gcgcctgttt gcgctcgatg ccgccagcgg caaagagaaa tggcattacg atcctgagct
      841 gaaaactaac gagtctttcc agcacgtaac ctgccgtggc gtctcttatc atgaagccaa
      901 agcagaaacg gcttcgccgg aagtgatggc ggattgcccg cgtcgtatca ttcttccggt
      961 caatgatggt cgactgattg cgattaacgc tgaaaacggc aaactgtgcg aaaccttcgc
     1021 caataaaggc gtgctcaatc tgcaaagcaa tatgccagac accaaaccgg gtctgtatga
     1081 accgacttcg ccaccgatta tcaccgataa aaccatcgtg atggccggtt cagttaccga
     1141 taacttctca acccgcgaaa cgtctggcgt gatccgtggt tttgatgtca acaccgggga
     1201 gctgctgtgg gcttttgatc ccggcgcgaa agatccgaac gcaatcccgt ctgacgaaca
     1261 cacctttacc tttaactcgc caaactcctg ggcaccagcg gcctatgacg cgaagctgga
     1321 tctggtctat ctgccgatgg gcgtgaccac gccggatatc tggggcggta accgcacacc
     1381 ggaacaggaa cgttatgcca gctcgattct ggcgctgaat gccactaccg ggaaactggc
     1441 gtggagctac cagaccgttc accacgacct gtgggacatg gatcttccgg cacagccgac
     1501 gctggcggac atcaccgtta atggtcagaa agtgccagtt atttacgctc cggcgaaaac
     1561 cggcaacatt tttgtgctcg atcgtcgcaa tggcgaactg gtggttccgg ccccggaaaa
     1621 accggtgccg caaggtgctg cgaaaggcga ttacgttact ccgacccagc cgttctctga
     1681 actgagcttc cgtccgaaga aagatttgag cggtgcggat atgtggggag ccaccatgtt
     1741 tgaccaactg gtgtgccgcg tgatgttcca ccagatgcgc tatgaaggca ttttcacgcc
     1801 gccatctgaa cagggcacgc tggtcttccc gggtaacctg ggaatgtttg aatggggcgg
     1861 gatttccgtt gatccaaatc gtgaagtggc aattgccaac ccaatggcac tgccgtttgt
     1921 ttcgaaactg atcccacgcg gtccgggtaa cccgatggag cagccgaaag atgccaaagg
     1981 cacgggtacg gaatccggca ttcagccaca gtacggtgta ccgtatggtg tcacgctcaa
     2041 cccgttcctc tcaccgtttg gtctgccgtg taaacagcca gcatggggtt atatctcggc
     2101 gctggatctg aaaatcaatg aagtggtgtg gaagaaacgt attggtacgc cgcaagacag
     2161 tatgccgttc ccgatgcctg taccggtgcc gttcaatatg ggtatgccga tgctgggcgg
     2221 gccaatctcc acggcgggta acgtgctgtt tatcgctgct acggcagata actacctgcg
     2281 cgcttacaac atgagcaacg gtgaaaaact gtggcagggc cgtttaccag cgggtggtca
     2341 ggcgacgcca atgacctatg aagtgaatgg caagcagtat gtggtgatct ccgcaggcgg
     2401 tcacggttca tttggtacga agatgggcga ctatattgtg gcttatgccc tgccggatga
     2461 tgtgaaataa ccataatgcc cggagagatc cgggcatatt tccgaatacg gtctttttat
     2521 accgtaaacc ctaacatcat ccccgtatct tcatgctcca gcagatggca gtgcgccata
     2581 taagcatgtt ctttcggtgc gttgtgatta aacttcacca gcacttcact gacattgcct
     2641 tctaccttaa cggtatcttt ccagcccgcg cgatgagccg ctggcggttt gccattttct
     2701 gacaagatac ggaactgcat gccgtgaata tggaacggat gcagcatcat gtcgcccacg
     2761 ccagagataa cccaacgttc gtactgccct ttcgccgccg caaacatcgg cttattcata
     2821 tcaaacgcct gaccgttgat tttattggca tggtggaaat cgaacttccc gccgtggttc
     2881 atatgattca tattgccgtg ccccatatgg cccatcatct ggctgtgatc cattccggcc
     2941 atcgcctgat cgccatattt ctccattagc atctgcatcc ccatcatatc gagcatcggg
     3001 tccatagaaa gttgcagctt gcgtaccgtc agcccttcca gcgaaggtaa cgcaggcagg
     3061 ctacttaatg tgtcaggcaa agcaccggag gcactaatag caatcggctg aatccgcatt
     3121 accggatgcg gcttgtcaaa cggcgcaatc gccatcccca tctggctgac cggtagcgtc
     3181 accagatcaa agggtttgtt gtcgttaacc tccaccagca cttcaaaacg ctcgcccatc
     3241 agcaccggca gctcgctcac cttcaccggt tcaggtagca gaccaccgtc gctggcaatc
     3301 acatacagcg ggcgattgtc gctggtggcg aaattgagcg agcgggcgtt acagccattg
     3361 agcaaacgca ggcgcagcca gccacgcggg gcagcgtgtt gtgggtagat tgcaccgttg
     3421 gtcagcagcg tatcgccaaa ccagcccacg gcggcggtca tcacatccag ttgataatca
     3481 atctgcccat cagcgttaaa ttttttatcc tgaacaatca ccggaacatc atcgataccc
     3541 cactgttttg gcagcattaa tttcaggatc tcgtcatctt caattaccac cagcccagcc
     3601 aaccccatcg ccacctgtcg cccggtcttg ccatgttgat gtggatggaa ccagcaggta
     3661 gcggcaggtt gatcaacgtt caacgtcacc gtacgtttgc cgcctggcgg aataattccc
     3721 tgcgggccgc cgtcgacgtc acccggtact tccagcccgt gccagtgcaa cgtcgtctct
     3781 tccgccagtt ggttatagat atcaaccgtt accgctttgc cgcgctgtaa tttcaccgcc
     3841 ggccccagca gattgccgtt atagccccag gtagttgcag ttttctcgcc aaaggtagac
     3901 tgacctgcgc caatagttaa ctgaatgcga ttacgggcat cggtcgtgag caaatcaggg
     3961 atcggtaacg ttgggcgttc cgccgcaaag actgcgcggc tccacagcgg caaggctgaa
     4021 gccacaccca gcgcgacgga atattttaag aaatcacgac gttgcatagt tatttcctta
     4081 ttcttaagca ggcgaaacaa aatcaaacgt tgagcatagt ccttcccctt acgggaaggt
     4141 caagccgcag acacaattta atcgacaata atggtcgtcg cctcgctcgc agtgtgctaa
     4201 cgtttatctt ctttaagccc ctggtagaag caatgaagac gtttttcaga acagtgttat
     4261 tcggcagcct gatggccgtc tgcgcaaaca gttacgcgct cagcgagtct gaagccgaag
     4321 atatggccga tttaacggca gtttttgtct ttctgaagaa cgattgtggt taccagaact
     4381 tacctaacgg gcaaattcgt cgcgcactgg tctttttcgc ccagcaaaac cagtgggatc
     4441 tcagtaatta cgacaccttc gacatgaaag ccctcggtga agacagctac cgcgatctca
     4501 gcggcattgg cattcccgtc gctaaaaagt gcaaagccct ggcccgcgat tccttaagcc
     4561 tgcttgccta cgtcaaataa tccctgatac ctttttgtag aaatattgac cgtgcatccg
     4621 cggtcaatgt tagctattat gttgcgccct ttttttacgg gtgttaacaa aggaggtatc
     4681 aacccatggc cgaaaaaaaa cagtggcatg aaacgctaca cgaccagttt gggcagtact
     4741 ttgcggtaga taacgttctg tatcatgaaa agaccgatca ccaagatctg atcatttttg
     4801 agaacgctgc atttggtcgc gtaatggcgc tggatggcgt agtacaaacc accgagcgcg
     4861 acgagtttat ctatcatgag atgatgaccc atgttccgct actggcccac ggtcacgcga
     4921 aacatgtgct gattatcggc ggcggcgacg gtgccatgct gcgtgaagta acccgacata
     4981 aaaacgttga gtcaatcacg atggtggaaa tcgatgcggg tatcgtgtcg ttctgccgtc
     5041 agtatctgcc caaccataac gccggtagct acgacgatcc gcgctttaag ctggtgatcg
     5101 acgatggcgt caatttcgtt aatcaaacca gccagacctt tgatgtcatt atctccgact
     5161 gcaccgatcc tatcggtccc ggcgaaagtc tgttcacctc agcattttat gaaggctgca
     5221 aacgttgcct gaatcctggc ggtatcttcg tcgcacaaaa cggcgtctgc tttttacagc
     5281 aggaagaagc catcgacagc tatcgcaaac tcagccatta cttcagcgac gttggctttt
     5341 atcaggcggc gatcccgacc tattacggcg gtatcatgac ttttgcatgg gcgacagata
     5401 acgacgcctt acgccatctc tcaaccgaaa ttattcaggc gcgttttctc gcttctggcc
     5461 tgaaatgccg ttattacaat ccggcaatcc atacggcagc ttttgcctta cctcagtatc
     5521 tgcaagacgc actggcttca cagccgtcct aaggagaaga taagaaattg aaaaaactga
     5581 aactgcatgg ctttaataat ctgaccaaaa gtctgagttt ttgtatttac gatatctgct
     5641 acgccaaaac tgccgaagag cgcgacggtt atattgctta tatcgatgaa ctctataatg
     5701 ccaaccgtct gaccgaaatc ctgtcagaaa cctgttccat tatcggggcc aatattctta
     5761 acatcgcccg ccaggattac gaaccacagg gtgccagcgt cactattctg gtgagtgaag
     5821 aaccggttga cccgaaactc atcgacaaaa cagaacaccc cggcccactg ccagaaacgg
     5881 tcgttgccca tctcgataaa agtcatattt gcgtacatac ctacccggaa agtcatcctg
     5941 aaggcggttt atgtaccttc cgcgccgata ttgaagtctc tacctgcggc gtgatttctc
     6001 cgctgaaggc gctgaattac ctgatccacc agcttgagtc cgatatcgta accattgatt
     6061 atcgcgtgcg cggttttacc cgcgacatta acggtatgaa gcactttatc gaccatgaga
     6121 ttaattcgat tcagaacttt atgtctgacg atatgaaggc gctgtatgac atggtggatg
     6181 tgaacgtcta tcaggaaaat atcttccata ccaagatgtt gcttaaagag ttcgacctta
     6241 agcactacat gttccacacc aaaccggaag atttaaccga cagcgagcgc caggaaatta
     6301 ccgctgcgct gtggaaagaa atgcgcgaga tttattacgg gcgcaatatg ccagctgttt
     6361 aacggctctg gcggagctcc caggctccgc cagatttatt tacttctgct gcacgaaatt
     6421 gcggtaagcc gccacgacct gcagaaaatc ctcaacgccg catagcgaca ggctttcttc
     6481 gtcgtagtag ttcatccctt cttccatttc atcgccagcg aattccagtt gattggcgcg
     6541 aaccatcacc tcttcaccgt ccatccacag ggtgtattca tgccctgccc gttgccagga
     6601 ccgttcgcta cctttcagtg cgtgcgctgc ttgttccact tcatcaagca aagccaggtt
     6661 ttctttcacc tcttcattaa accagtgccc gaccacttca tgccccatgg acatacgcac
     6721 ctttaccact ccggtaatat cgcgcagaaa ttcgtaatcc atagtgtgtt cctctgctcc
     6781 cggcaatgcg ccgaaatcgc attgcgctat tgatgtaatt attgcagcct taccggagaa
     6841 aaacagcgga acggcgatgg tttagaaata aaaaatgccc gggaatctca ttcaccgggc
     6901 attgtgtcgt ttatgcgcag cgcgtgcgct tacttattac accgcagtct ggaaaatcac
     6961 cccatcggct ttttcggtgt actgagaaag ctggttgaag ttcagataac gataagtatc
     7021 aacggcagtt ttatctactt gcgcaacgta ggtctggtac tcttccggcg tcggcagttt
     7081 gccaatcagc gccgcaacag ccgccagttc tgcagaagcc aggaagacat tcgcgccagt
     7141 ccccagacgg ttcgggaagt tacgggtaga ggtggaaacc accgtcgcac cgtctgctac
     7201 acgcgcctgg ttacccatac acagagaaca gcccgggatc tcgatacgag ccccgctctt
     7261 accgaagacg ctgtagtagc cttcttcagt caactgtgcg gcgtccatgc gggttggcgg
     7321 tgccacccac aggcgggtcg gcaactggcc tttgtgcgca tccagcagtt tacccgcagc
     7381 acggaagtga ccgatgttag tcatgcaaga accgataaac acttcgtcga tcttctcgcc
     7441 ctgtaccgca gacagcggac gtgcgtcgtc cgggtcgttc ggtgcacaga ggattggctc
     7501 tttaatatcc gccagatcga tgtcgatcac tgccgcgtat tcagcatctg catcggcttc
     7561 cagcagctca ggattcgcca gccatttttc catgccctga atacgacgtt cgagggtacg
     7621 acgatcgcca taaccttccg cgatcatcca cttcagcagg acgatgttag agttcaggta
     7681 ttcgatgatc ggttctttgt tcagcttgat agtacaacca gcggcagaac gctcagcgga
     7741 cgcatcggtc agttcaaatg cctgctcaat tttcagatcc ggcagacctt caatttccag
     7801 aatgcgacca gagaagatgt ttttcttgcc tttcttctca acggtcagca gaccttgttt
     7861 gatcgcatac agcgggatcg cgtgtaccag atcgcgcagg gtgatgcccg gctgcatttt
     7921 gcctttgaag cgcaccagaa cggattccgg catatccagc ggcattacgc ctgttgcggc
     7981 tgcaaatgcc accagaccag aacccgccgg gaaagagata ccgatcggga aacgggtatg
     8041 ggagtcacca ccggtaccga cggtatccgg cagcagcata cggttcagcc aggagtggat
     8101 tacgccgtca cccggacgca gcgacacacc accacggttc ataatgaagt ccggcagcgt
     8161 gtggtgcgtg ttcacgtcaa ctggcttcgg atacgccgcg gtatggcaga aagactgcat
     8221 caccaggtca gccgagaagc ccaggcacgc caggtctttc agttcatcac gggtcatcgg
     8281 gccggtggtg tcctgtgaac ctacagaagt cattttcggt tcgcagtacg cgcccggacg
     8341 aatacctttc acaccgcagg cacgacctac cattttctga gccagcgaga agccgcgatc
     8401 gctctcagcg acatctttcg cctgacggaa cacatcactg tgcggcagac caagtgcttc
     8461 acgcgctttg gtggtcaggc cacgcccgat aatcagcgga atacggccac cagcacgcac
     8521 ttcatcaatc agcacgtcgg ttttcagttc gaaggtcgcc agcagttcgc cggtttcgtg
     8581 gttacgcact tcacctttgt acgggtaaac gtcaatcacg tcgcccatgt tcaggttaga
     8641 gacgtcgact tcgattggca gtgcacccgc gtcttccatc gtgttaaaga agatgggtgc
     8701 aattttaccg ccgaggcaca aaccaccgcc gcgtttgttc ggcacatgtg gaatatcatc
     8761 gcccataaac cacagaacgg agttcgtggc ggatttacgc gatgaacccg tacccacaac
     8821 gtcaccgacg tacgccagcg ggaaaccttt ctgttgcaga gcttcgattt gcttgatcgg
     8881 gccaacaaca cctggctggt ctggctcaat accttcacgg gcgtttttca gcatcgccag
     8941 cgcgtgcagt gggatatccg ggcgcgacca cgcatccggt gccggagaaa ggtcatcagt
     9001 gttggtttcg ccagtgactt tgaagacggt aacggtcagt ttttcagcca gcgccgggcg
     9061 attcaggaac cattcggcat ccgcccagga ctgcattacc tgcttcgcat attcgttgcc
     9121 tgctttggct ttctcttcta cgtcatagaa gttatcgaac atcagcagtg tgtgagaaag
     9181 tgctttggca gcgatcggtg ccagtttggc atcatccagc gcgtcgatca gcggatgaat
     9241 gttgtaacca ccctgcatgg tacccagcag ttcgatggct ttttccggag tcagcagagg
     9301 ggatttggct tcgcctttag cgatagcagc caggaagcct gctttgacat aggcggcttc
     9361 atcgacgcca gggggaacac ggttggttaa tagatctaac aggaattctt cttcgcccgc
     9421 gggcgggttt ttcagcagct ctacaagtgc ggccatttgg tttgcatcca ggggtttggg
     9481 cgcaatcccc tcagcggcac gctcagctac gtgcttacgg tattcttcta gcacgacggt
     9541 tctcctcgct ctcattgtca tagtgcggca ggcgattctc ttcacgctcc tgtgagacag
     9601 cagtttgtag ggtaaatgcc cagtaccgca acgggcagaa tagcaggatt ttgacgacgt
     9661 gttaatctgt ttacaaaaaa gcaacataaa aaagtggctg aatcgttaag gatggtctag
     9721 agattgtttc cctccgtaaa aacttcgtta attcgcatgg tgataatcac ctttctcaac
     9781 gaatcccaag attagtcaaa atttaaacta ccgcctcttt atactcggat taacagcacc
     9841 tgcgggtggc agttcgccga ccattgcgat ttccttgaga tccgaattat gaaaatgact
     9901 ttgccgttta aaccccatgt gctggcacta atttgcagtg ccgggctttg tgccgcctct
     9961 gccgggctat atataaaaag ccgcacagtg gaagcgcctg tggaaacgca atcgacacaa
    10021 cagactgcgc ctgacatcac cgcagttacg cttcctgcaa cggtttccgc accgcccgta
    10081 acgcccgccg tcgtcaaatc cgcattcagc actgcacaaa tcgatcaatg ggttgcgcct
    10141 gtcgcgctgt accccgattc tctgctttca caagtgttaa tggcatcaac ctatccggca
    10201 aacgttgctc aagcagtgca atggtcgcac gataatccac ttaaacaagg cgatgctgct
    10261 attcaggcgg tatctgacca gccgtgggac gccagcgtta aatcactggt ggcctttcca
    10321 caattgatgg cattgatggg cgaaaacccg caatgggtgc aaaacctggg cgatgctttt
    10381 ctggcgcagc cgcaggacgt gatggactcg gtacagcgat tgcggcaact ggcgcaacaa
    10441 accggctcgc tgaagtcatc aaccgaacag aaagttatta ccacaacgaa gaaagctgta
    10501 ccggtaaaac agacagtcac ggcacccgtc ataccatcca ataccgtttt aactgccaac
    10561 cccgtcatta cagagccggc aacaaccgtc atttccattg agcccgccaa tcctgatgtg
    10621 gtctatattc ccaactacaa cccaaccgtg gtttacggga actgggccaa tactgcgtat
    10681 ccgccggttt atctgccacc accagccgga gaaccgtttg ttgacagctt tgtgcgcgga
    10741 ttcggctata gcatgggcgt tgctaccacg tacgcactat ttagcagcat cgactgggat
    10801 gacgacgatc atgaccatca tcatcatgac gatgatgatt atcatcacca cgatggcggt
    10861 catcgtgacg gtaatggctg gcaacacaac ggcgacaaca tcaatatcga cgtcaacaat
    10921 ttcaaccgta tcaccggtga gcatcttact gataagaata tggcatggcg gcacaatcca
    10981 aactaccgta atggtgtgcc ctatcatgat caggatatgg caaagcggtt tcatcaaacc
    11041 gatgtcaacg gcggaatgag tgccacgcag ctacctgctc caacacgcga cagccagcgt
    11101 caggcggcag caagtcagtt tcagcaacga acacacgccg cccccgtcat tacacgagat
    11161 acccaacgtc aggcagcggc acagcggttt aatgaagctg aacactatgg gagctatgac
    11221 gacttccgcg acttcagccg tcgccaacca ctgacccagc aacaaaagga cgccgctcgt
    11281 cagcgttatc aatcggcctc tcctgagcag cgccaggcgg ttcgcgagaa aatgcagact
    11341 aacccacaga accagcagcg aagagaggca gcgcgtgagc gtattcagtc tgcaactccc
    11401 gaacagcgcc aggtgtttaa ggaaaaagta cagcagcgcc cactgaacca acagcaacgt
    11461 gataacgccc gccagcgtat acaatcggca tcacctgaac aacgtcaggt ttttcgggaa
    11521 aaagttcagg agagccgccc acaacgtcta aacgacagta accatactgt caggctgaat
    11581 aacgagcaac ggtcagcagt acgcgaacgt ctctctgagc gcggagcaag gcgacaggaa
    11641 aggtaaattg cagacgtaaa aaaagcggcg tggttagccg cttttttaat tgccggatgt
    11701 tccggcaaac gaaaaattac ttcttcttcg ctttcgggtt cggcaggtcg gtaatgctac
    11761 cttcgaacac ttctgccgcc aggcccacag actcgtgcag agtcgggtgc gcgtggatgg
    11821 tcagtgcgat gtcttcagca tcacaaccca tttcgattgc cagaccgatt tcacccagca
    11881 gctcaccgcc gttagtaccg acaatcgcac caccgatcac acggtgagat tctttgtcga
    11941 aaatcagctt ggtcataccg tctgcgcagt cggaagcgat agcacgacca gaagcagccc
    12001 acgggaaggt ggcggtttca tagctgatgc ctttctcttt cgcttctttc tcagtcagac
    12061 ctacccatgc aacttctggt tcggtatagg cgatagacgg gataactttc ggatcgaagt
    12121 agtgtttttt accggcgata acttcagcgg caacgtgacc ttcgtgaaca cctttgtgtg
    12181 ccagcatcgg ctgaccaacg atatcgccga tagcaaagat gtgcggtacg ttggtacgca
    12241 gctgtttgtc aacgcggatg aaaccacggt cgtccacttc cacgccagct ttgcctgcgt
    12301 cgaggttttt accgttcggc acacgaccaa tcgctaccag cacggcgtcg taacgctgcg
    12361 gttcagcggg tgcttttttg ccttccatcg tcacataaat accgtcttct ttcgcttcaa
    12421 cggcggtaac tttggtttcc agcatcaggt tgaatttctt gctgatacgc ttggtgaaga
    12481 ctttaacgat gtctttgtca gctgccggga taacctggtc gaacatttca accacgtcaa
    12541 tctgtgaacc cagcgcgtgg tatacggtgc ccatttccag accgatgata ccgccaccca
    12601 ttaccagcag gcgttctggt acttctttca gttccagcgc gtcagtggag tcccagatac
    12661 gcggatcttc atgcggaata aacggcagtt ggatcgggcg agaacccgct gcaatgatcg
    12721 cattgtcgaa gttgatcacg gttttgccgt tctcaccttc aacttccagg gtgttagccc
    12781 cggtgaattt acccagaccg ttgaccactt tgactttgcg gcctttcgcc ataccagcca
    12841 gaccaccggt cagctgattg atcactttct ctttccaagt acgaatcttg tcaatatcgg
    12901 ttttcggttc gccgaagacg ataccgtgtt cagccagcgc tttggcttct tcgataactt
    12961 ttgctacgtg cagcagtgct ttagaaggga tacagccgac gttcaggcaa acaccgccaa
    13021 gggtgttgta acgttctacg attacggttt ccagacctaa atcagcgcaa cggaaggcag
    13081 cggagtaacc tgcggggcct gccccaagta ccacgacctg agttttgatt tcagtactca
    13141 tcatgacctc tatatattta tctccggcgg tcatacccgt cgtctttcag gccgcagcgg
    13201 cgttagcttc acgccctcac cccagtcact tacttatgta agctcctggg gattctggcg
    13261 cttgctacct tgctgcaccc agaaatccat agggtatgtg aagtaatacc ctaaccacca
    13321 ccgggtcgtt ctatccgtcg gtattttaca aaattgttaa caatttttaa acaacaaacg
    13381 gcaaccgatt tgtctattcg ctaataacct caatacattc atgagattac cagaaaaaag
    13441 ccggccgttg ggccggctct tttacttaca tcaccagacg gcgaatgtca gacagcgtgt
    13501 tgttaatgat ggtaatgaaa cgggcaccat cagcaccgtc gatcacgcgg tggtcgaagg
    13561 agagagaaat cggcagcatc agacgcggca cgaactcttt accattccac accggctcca
    13621 tcgcggactt ggaaacgccg aggatagcca cttccggcgc gttcacaatc ggcgcgaagt
    13681 gggtagtacc caggccgccg atgctggaga tggtgaagca accgccctgc atttcgcccg
    13741 cagtcagctt accgtcacgc gctttcttag aaatagtcat cagctcgcga gacagctcga
    13801 tgatgccttt cttgttgacg tctttgaata ccggaacaac cagaccgttc ggggtatcca
    13861 ccgccacacc gatgttgatg tatttcttca gggtcagacg ctgaccgtct tccgacagcg
    13921 aactattgaa gcgaggcatc tgctcaagag ctgcagcaac ggctttcatg atgaagacaa
    13981 ccggggtgag cttcacatcc agcttacgtt tcgccgcttc ttcgttctgc tgtttacgga
    14041 acgcttccag ctcggtgata tcggttttgt cgaagtgggt aacatgcggg atcatcaccc
    14101 agttacggct caggttagca ccagaaattt tctggatacg gcccagttcc acttcttcga
    14161 tttcgccaaa cttgctgaag tccaccttcg gccacggcag catgccaggg ataccaccgc
    14221 cagtcgccgc cggagctgct tctgcacgtt tgatagcttc tttcacgtaa gcctgaacgt
    14281 cttcgcgcag gatacgacct ttacgaccag tgcccttcac tttcgccagg ttaacgccaa
    14341 actcgcgtgc cagacggcgg atcagcggag tcgcgtgaac gtaagcgtcg ttttcagcaa
    14401 attcagattt gccttcggct ttcgcagccg gagctgctgc cggggcttca gctttcgctg
    14461 ccggagccgg cgctgccgct tcctgtttcg caggagctgc cgcaggcgct gcgccttcaa
    14521 cttcgaagat cataatcagc gagccagttt tcactttatc gccaacgttg actttcagtt
    14581 ccttcacgac gcctgcaaac ggagccggga cctccataga agctttatcg ccttctacgg
    14641 tgataagcga ttgttcagcg gcaactttgt cgcccacttt caccatcact tcggtcactt
    14701 caacttcgtc accgccgata tccggaacgt taacttcttt cacgccagcc gctgatgcag
    14761 gggccgctgc cggagctgct tcctgtttag ccgccggagc tgccgcgcct gcttcacccg
    14821 cgacttcgaa gaccataatc agcgagccgg tagacacttt gtcacccacg ttcactttga
    14881 tctctttcac ggtgccagca aacggagccg gaacttccat agaagcttta tcgccttcta
    14941 cggtgatcag cgactgttca gcttcaactt tatcgccaac tttcaccagg atttcggtca
    15001 cttcaacttc gtcgctgccg atatccggaa cgttaacgtc ttttgccgcc gcagccgcgg
    15061 gtgctgctgc cggagctgct tctttcttct cttctgcctg agcaggtgca gcgtctgctg
    15121 caccgtcggc ggaatcgaaa atcataatca gtgcgccggt ctgggtttta tcgccaacag
    15181 agactttgat ctctttaacg atacccgcct gcggagacgg aacttccata gaggctttgt
    15241 cgccttctac ggtgatcagc gactgttcgg cttcaacttt gtcgcccact ttgaccagga
    15301 tctcggtgat ttcaacttca tcagccccga tgtccggtac tttgatttcg atagccatta
    15361 ttcttttacc tcttacgcca gacgcgggtt aactttatct gcatcgatgt tgaatttggc
    15421 gattgcgtca gcaaccactt tcttatcgat ttcgccacgt ttagccagtt cgcccagcgc
    15481 cgcaaccacg acataagaag catcaacttc gaagtggtga cgcaggttct cacggctgtc
    15541 ggaacgaccg aagccatcag tacccagtac gcggtagtcg tcagccggta cgtaagtacg
    15601 gacctgctca gcgaacagtt tcatatagtc ggtagatgcc actgccggag cgtcgttcat
    15661 cacctgagcg atatacggaa cgcgcggagt ttccagcgga tgaagcatgt tccagcgttc
    15721 acaatcctga ccatcacgcg ccagctcggt gaaggaggtc acgctataaa cgtcagaacc
    15781 tacgccgtaa tctttcgcca ggatctctgc cgcttcacgg acgtgacgca ggatagaacc
    15841 ggagcccagc agctgaactt tacctttgct accttcaatg gtttcgagtt tgtagatacc
    15901 tttacggata ccttcctcag caccttccgg cattgccggc atgtggtagt tttcgttcag
    15961 cgtagtgatg tagtagtaaa cgttctcttg tttttcaccg tacatacgct ccagaccgtc
    16021 atgcatgatg acagcaactt cgtaagcgta agccgggtcg taagagatac agttcgggat
    16081 agtcagcgac tgaatgtggc tgtgaccatc ttcgtgctgc agaccttcac cgttcagggt
    16141 ggtacgaccg gaagtaccgc cgatcaggaa gccacgcgct tgctggtcgc cagccgccca
    16201 gcacagatcg ccgatacgct ggaagccgaa catcgagtaa tagatgtaga acggaatcat
    16261 cggcagattg ttggtgctgt aagaggtcgc cgctgccagc caggaacacc ctgcgcccag
    16321 ctcgttgatc ccttcctgca gaatctgacc tttctcgtct tctttatagt aagcaacctg
    16381 ctcgcggtcc tgcgggatgt actgctgacc gttcgggctg taaataccaa tctgacggaa
    16441 cagaccttcc ataccgaaag tacgcgcttc gtcggcgatg atcggtacca gacgatcttt
    16501 gatcgacttg ttcttcagca ttacgttcag ggcacgaacg aaagcgatag tggtagagat
    16561 ctctttgctc tgctcttcca gcagcgcgcc gaagtcttgc aggctcggca gctcaagctt
    16621 ctcggtgaag ttcggctgac ggcttggcag ataaccgtgc agtttctgac gctgtgcgtg
    16681 cagataggta tgctcttcag aaccttccgg gaaggtgatg tacggcagtt tttcgatatc
    16741 tgcatcagac accggcacat tgaaacggtc gcggatgtga cgcacgccgt ccatgttcat
    16801 tttcttaacc tggtgcgcga tgtttttacc ttcagccgcg tcgcccatgc cgtaaccttt
    16861 aatggtatga gcaaggatta ctgtcgcttt gcctttggtt tcctgcgctt tcttgaatgc
    16921 agcgtagatt ttcttcggat cgtgaccacc acggttcagt gcccagatct gctcgtcagt
    16981 ccagtctgca accagtgctg cggtttcagg atatttaccg aagaagtgtt cacgaacgta
    17041 cgcaccatct ttcgatttga aggtctggta gtcgccgtca acggtttcgt tcatcagctg
    17101 gatcagttta ccgctggtat ctttacgcag cagttcatcc caacggctac cccacatcac
    17161 tttgataacg ttccagccag caccttcgaa gatgccttcc agttcgttga tgatcttgcc
    17221 gttaccggtg accgggccgt caagacgctg caggttacag ttgataacga agaccaggtt
    17281 atccagtttt tcacgggtag cgatggtgat cgcacctttg gattccggtt cgtccatttc
    17341 accgtcgccg aggaacgcgt aaacggtctg tttagaggta tctttcaggc cacggtgttc
    17401 cagatatttc aggaatttag cctggtaaat agcaccaatc ggccccagac ccatagatac
    17461 ggtcgggaac tgccagaatt ccggcatcag tttcgggtgc ggataggaag agaggccatt
    17521 gccgtgaact tcctgacgga agttatccag ctgctcttga gtcagacgac cttccaggaa
    17581 agcacgagcg tacacgcccg gggagatgtg gccctggaag taaaccaggt cgccgccatc
    17641 ctgctcgttg cgtgcacgga agaagtggtt aaagcacaca tcataaatgg ttgcggaaga
    17701 ctggaaggac gccatatggc cgcccagttc gaggtctttt ttcgacgcac gcagcaccgt
    17761 catgatggcg ttccagcgga tagctgaacg aatacggcgt tccagttcca gattacccgg
    17821 atactccggt tgttcttcaa cggggatggt gttgatgtag ttgctgatac ctgtgcctgc
    17881 ggctacgttt acaccgcctt tgcgggcttc agcaagcagt tggtcgatca gatactgagc
    17941 acgctcaaca ccttcttcac ggatgaccga ttcgatcgcc tggagccagt cgcgagtttc
    18001 gatcggatcc acgtcatttg ggaaacgttc tgacatgggt tattccttat ctatctaata
    18061 acgttgagtt ttctggaacc tgtcccattg aactctcgcc ggaaagctca ataagacagg
    18121 ttctacgttt agttgccgcg cttttatata cgcttgattt acaacatctt ctggataatt
    18181 tttaccagaa aaatcactaa ttctttcgtt gctccagacg acgcagagaa cgctcacggc
    18241 ggctctcttc acgacttctg tcgagcaaaa tttcttcgat aaaggccaga tggcgatgcg
    18301 atgcttcgcg cgcttcttcc ggcttaccgg ccataatcgc ttcaaatatg cgggtgcggt
    18361 gactactcac cagcggcagc atctcgcgac gcgaatagag caattcgaag ttctggcgga
    18421 cattctgggc caacatcggc tccatacacc ttagcagatg aagcagaacc acattgtggg
    18481 ccgcttcggt gacggcaatc tgatactgga gtacggcgtt tgattccgcg tccagatcgc
    18541 cagactgctg cgccagctct atggcgtggt ggagttcacg gatgcgttcc ttgtcttcat
    18601 cggtactacg cagcgcggcg taataagcgg cgataccttc cagggcgtgt cgtgtttcga
    18661 gcaagtcata ctgtgattca ggatggtcgg agagcagctc caccagcgga tcgctgaagc
    18721 tttgccatag gctgctctgg acaaaagtgc cgccaccctg gcgacgaagc aacaagccct
    18781 tcgcttcgag acgttgaatc gcctcacgca aggagggacg ggagacgtcg aactgttttg
    18841 ccagttcgcg ttccggtggg agtttttcgc ccgggcggag agtgccttcg agaatcaaaa
    18901 actccagttg ctgctcaatc acatcggaga gttttggttg gcggattttg ctgtaggcca
    18961 tgagttcctg tcttaagcca cttgccgaag tcaattggtc ttaccaattt catgtctgtg
    19021 acgctaaagt aacaaagtat tcaccttatg tccatacagg ttttgattga aatcatgaaa
    19081 ctgtgcacat t
//
LOCUS       DAZOYL010000053        18638 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZOYL010000053 DAZOYL010000000
VERSION     DAZOYL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18638
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.053"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHUOY_001348"
     CDS             complement(<1..186)
                     /locus_tag="ACHUOY_001348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0682171.1"
                     /translation="MFIVLFLHFCHELQLSGMVVLNTTHPATAKTFDPERHAVQLVNK
                     CPRFPRRAITLLQMQMVI"
     gene            179..2419
                     /gene="fepA"
                     /locus_tag="ACHUOY_001349"
     CDS             179..2419
                     /gene="fepA"
                     /locus_tag="ACHUOY_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR0682172.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEINPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            2594..3214
                     /gene="entD"
                     /locus_tag="ACHUOY_001350"
     CDS             2594..3214
                     /gene="entD"
                     /locus_tag="ACHUOY_001350"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR0682173.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(3336..3488)
                     /gene="hokE"
                     /locus_tag="ACHUOY_001351"
     CDS             complement(3336..3488)
                     /gene="hokE"
                     /locus_tag="ACHUOY_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0682174.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(3839..3991)
                     /gene="hokE"
                     /locus_tag="ACHUOY_001352"
     CDS             complement(3839..3991)
                     /gene="hokE"
                     /locus_tag="ACHUOY_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0682175.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            3960..4228
                     /locus_tag="ACHUOY_001353"
                     /pseudo
     CDS             3960..4228
                     /locus_tag="ACHUOY_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4431..5549
                     /locus_tag="ACHUOY_001354"
     CDS             4431..5549
                     /locus_tag="ACHUOY_001354"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR0682176.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            5615..5863
                     /locus_tag="ACHUOY_001355"
                     /pseudo
     CDS             5615..5863
                     /locus_tag="ACHUOY_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            5928..6296
                     /locus_tag="ACHUOY_001356"
     CDS             5928..6296
                     /locus_tag="ACHUOY_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0682177.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            6390..7043
                     /gene="nfsB"
                     /locus_tag="ACHUOY_001357"
     CDS             6390..7043
                     /gene="nfsB"
                     /locus_tag="ACHUOY_001357"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR0682178.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            7151..8398
                     /gene="ybdG"
                     /locus_tag="ACHUOY_001358"
     CDS             7151..8398
                     /gene="ybdG"
                     /locus_tag="ACHUOY_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR0682179.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(8466..9842)
                     /gene="pheP"
                     /locus_tag="ACHUOY_001359"
     CDS             complement(8466..9842)
                     /gene="pheP"
                     /locus_tag="ACHUOY_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR0682180.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(9944..13087)
                     /gene="cusA"
                     /locus_tag="ACHUOY_001360"
     CDS             complement(9944..13087)
                     /gene="cusA"
                     /locus_tag="ACHUOY_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR0682181.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(13099..14322)
                     /gene="cusB"
                     /locus_tag="ACHUOY_001361"
     CDS             complement(13099..14322)
                     /gene="cusB"
                     /locus_tag="ACHUOY_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR0682182.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(14338..14670)
                     /gene="cusF"
                     /locus_tag="ACHUOY_001362"
     CDS             complement(14338..14670)
                     /gene="cusF"
                     /locus_tag="ACHUOY_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR0682183.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(14694..16076)
                     /gene="cusC"
                     /locus_tag="ACHUOY_001363"
     CDS             complement(14694..16076)
                     /gene="cusC"
                     /locus_tag="ACHUOY_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR0682184.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            16233..16916
                     /gene="cusR"
                     /locus_tag="ACHUOY_001364"
     CDS             16233..16916
                     /gene="cusR"
                     /locus_tag="ACHUOY_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR0682185.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            16906..18354
                     /gene="cusS"
                     /locus_tag="ACHUOY_001365"
     CDS             16906..18354
                     /gene="cusS"
                     /locus_tag="ACHUOY_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR0682186.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         4587 a         4955 c         4652 g         4444 t
ORIGIN      
        1 gataaccatt tgcatttgca atagcgtaat ggcgcgccgt gggaagcgcg gacatttatt
       61 aaccaactgc actgcgtgtc tttcaggatc aaaggttttc gcggtagcgg gatgcgtcgt
      121 gttgaggacg accatgcccg acagttgcaa ttcgtggcaa aaatgcagga ataaaacaat
      181 gaacaagaag attcattccc tggccttgtt ggtcaatctg gggatttatg gggtagcgca
      241 ggcacaagag ccgaccgata ctcctgtttc acatgacgat accattgtcg ttaccgccgc
      301 cgagcagaac ttacaggcgc ctggcgtttc gaccatcacc gcagatgaaa tccgcaaaaa
      361 ccccgttgcc cgcgatgtgt cggagatcat ccgtaccatg ccaggcgtta acctgaccgg
      421 taactccacc agtggtcagc gtgggaataa ccgacagatt gatattcgcg gtatgggtcc
      481 ggaaaacacg ctgattttga ttgacggcaa gccggtaagc agccgtaact cggtgcgtca
      541 gggctggcgt ggcgagcgcg atacccgtgg tgatacctcc tgggtgccac ctgaaatgat
      601 tgaacgtatt gaagttctgc gtggtccggc agctgcgcgt tatggcaacg gcgcggcggg
      661 cggcgtggtt aacatcatta ccaaaaaagg cagcggtgag tggcacggct cctgggacgc
      721 ttatttcaat gcaccagaac ataaagagga aggtgccact aaacgcacca acttcagcct
      781 gaccggtccg ctgggcgacg aattcagctt ccgcttgtat ggcaacctcg acaaaaccca
      841 ggctgacgcg tgggatatca accagggcca tcagtccgcg cgtgccggaa cgtatgccac
      901 gacgttacca gccgggcgcg aaggggtgat caacaaagat attaatggcg tggtgcgctg
      961 ggatttcgcg ccattgcaat cgctggaact ggaagcaggt tacagccgcc agggcaacct
     1021 gtatgcgggc gacacccaga acaccaactc cgattcctat acccgctcga aatatggcga
     1081 tgaaaccaac cgtctgtatc gccagaacta ctcgctgacc tggaacggcg gctgggataa
     1141 cggagtgacc accagcaact gggtgcagta tgaacacacc cgtaactcgc gtattccgga
     1201 aggtctggca ggcggtaccg aagggaaatt taacgaaaaa gcgacacagg atttcgtcga
     1261 tatcgatctt gatgacgtga tgctgcacag cgaagttaac ctgccgattg atttcctcgt
     1321 taaccaaacg ctgacgctgg gtacggagtg gaatcagcaa cggatgaagg acttgagttc
     1381 caatacccag gcgctgaccg gggcgaacac cggcggcgct attgatggtg tgagtgccac
     1441 cgaccgtagc ccgtattcaa aagcagaaat tttctcgctg tttgccgaaa acaacatgga
     1501 gctgactgac agcaccatcg taacgccggg gctgcgtttc gatcatcaca gtattgtcgg
     1561 caataactgg agcccggcgc tgaacatatc gcaaggttta ggcgatgact tcacgctcaa
     1621 aatgggcatt gcccgtgctt ataaagcacc gagcctgtac cagactaacc cgaactacat
     1681 tctctacagt aaaggtcagg gctgctatgc cagcgcgggc ggctgctatc agcaaggtaa
     1741 cgatgacctg aaagcagaaa ccagcatcaa caaagagatt ggtctggagt tcaaacgcga
     1801 cggctggctg gcgggcgtca cctggttccg taacgattat cgcaataaga ttgaagcggg
     1861 ctatgtggct gtagggcaaa acgcagtcgg caccgatctc tatcagtggg ataacgtgcc
     1921 gaaagcagtg gttgaaggtc tggaaggatc gttaaacgta ccagttagcg aaatggtgat
     1981 gtggaccaat aacatcactt atatgctgaa gagtgaaaac aaaaccacgg gcgaccgttt
     2041 gtcgatcatc ccggagtaca cgttgaactc aacgctgagc tggcaggcgc gggaagattt
     2101 gtcgatgcaa acgaccttca cctggtacgg taagcagcag ccgaaaaaat acaactacaa
     2161 aggtcagcca gcggttggcc cggaaaccaa agaaatcaat ccttacagca ttgtgggtct
     2221 gagcgcgacc tgggatgtga cgaagaatgt cagtctgacc ggcggcgtgg acaacctgtt
     2281 cgacaaacgt ttgtggcgtg cgggtaatgc ccagaccacg ggcgatctgg caggggccaa
     2341 ctatatcgcg ggtgccgggg cgtataccta taacgagccg ggacgtacgt ggtacatgag
     2401 cgtaaacact cacttctgat gctaacgtca gattgttgac aaagtgcgcg tcgttcatgc
     2461 cggatgcggc gtgaacgcct tatccggcct acaaaaatct tgccaattca atatattgca
     2521 ggaccatgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgt catcagtctc
     2581 gaatatggtc gatatgaaaa ctacgcatac ctccctcccc tttgccggac atacgctgca
     2641 ttttgttgag ttcgatccgg cgaatttttg tgagcaggat ttactctggc tgccgcacta
     2701 cgcacaattg caacacgctg gacgtaaacg taaaacagag catttagccg gacggatcgc
     2761 tgctgtttat gccttacggg aatatggcta taaatgtgtg cccgcaatcg gcgagctacg
     2821 ccaacctgtc tggcctgcgg aggtatacgg cagtattagc cactgtggga ctacggcatt
     2881 agccgtggta tctcgtcaac cgattggcat tgatatagaa gaaatttttt ctgtacaaac
     2941 cgcaagagaa ttgacagaca atattattac accagcagaa cacgagcgac tcgcggactg
     3001 tggtttagcc ttttctctgg cgctgacact ggcattttcc gccaaagaga gcgcatttaa
     3061 ggcatcggag agacaaactg aagcaggttt tctggattat cagataatta gctggaataa
     3121 acagcaggtc atcattcatc gtgagaatga gatgtttgct gtgcactggc agataaaaga
     3181 aaaaatagtc ataactctgt gccaacacga ttaattgaca acatctggta cgattcgccc
     3241 gcagccatca ctgactgcgg gcgaaagtgt aaagcaggtg ccttaccact ctgacctgac
     3301 aaccggatat gcagggattg ctccccgcac aacggctact tcttcggttc gtaagcgaga
     3361 acagccttaa actcaatatt acgttccttt acggtgaact cacacagcga gtctccgacc
     3421 agaagcgtaa atcccagcac cgtcaaacac agcactatga ctgccacaag ggcatatttc
     3481 gtcagcatgt ttatattgcc tccatgcgaa gaagggacta ccatccgagt tgttgaggtt
     3541 cggagtggca gccccaggat tgatagaaat atctcctggg gcttcatctt tccagacctc
     3601 agagtaaacc tgaaaccgga aagcttcagg cacccgccgt tatcttaccc ttcctcagca
     3661 aaatatctgt ttatttatac agtaagtcct gtttcggact tctgttggca acactcgtta
     3721 ttgctcgtaa aacgaaatcg cccgcagcca tcactgaccg cgggcgaaag tgtaaagcag
     3781 gtgccctacc atcctgacct gacaaccgga tatgcgggga ttactccccg cacaacggct
     3841 acttcttcgg ttcgtaagcg agaacagcct taaactcaat attacgttcc ttcaccgtaa
     3901 actcacacag cgagtctccg accagaagcg taaatcccag caccgtcaaa cacagcacta
     3961 tgactgccgc aagggcatat ttcgtcagca tgtttatatt gcctccatgc gaagaaggga
     4021 ctaccatcca aattgttcgg gtttggagtg gcagccccag ggttgataga aatatctcct
     4081 ggggcttcat ctttccagac ctcagagtaa acctgaaacc ggaaagcttc aggcacccgc
     4141 cgttatctta cccttcctca acaaaatggc tgtttattta tacagtaaat cctgtttcgg
     4201 acttctcact cctcatttaa tcaactgaag ctgagcccgt tttttctgca tgcctcaatc
     4261 gtcaaaattc ttcgccatca gccagctctg ccataacttg ccacattgaa aaagtagcca
     4321 aattgcttca gtcaccatca tcaaaaaaag aaacattcca gaacttccgc atctcgccca
     4381 gccgctccga cttctatact gaatagaaaa cgccaacata agagaaacct atgccattac
     4441 ccgattttca tgtttctgaa ccttttaccc tcggtattga actggaaatg caggtggtta
     4501 atccgccggg ctatgactta agccaggact cttcaatgct gattgacgcg gttaaaaata
     4561 agatcacggc cggagaggta aagcacgata tcaccgaaag tatgctggag ctggcgacgg
     4621 atgtttgccg tgatatcaac caggctgccg ggcaattttc agcgatgcag aaagtcgtat
     4681 tgcaggcagc cgcagaccat catctggaaa tttgcggcgg tggcacacac ccgtttcaga
     4741 aatggcagcg tcaggaggta tgcgataacg agcgctatca acgaacgctg gaaaactttg
     4801 gctatctcat ccagcaggcg accgtttttg gtcagcatgt ccatgttggc tgcgccagtg
     4861 gcgatgacgc catttatttg ctgcacggct tgtcacggtt tgtgccgcac tttatcgccc
     4921 tttccgccgc gtcgccatat atgcaaggaa cggatacgcg ttttgcctcc tcacgaccga
     4981 atattttttc cgcctttccc gataatggcc cgatgccgtg ggtcagtaac tggcaacaat
     5041 ttgaagccct gtttcgctgt ttgagttaca ccacgatgat cgacagcatt aaagatctgc
     5101 actgggatat tcgccccagt cctcattttg gcacggtgga ggttcgggtg atggataccc
     5161 cgttaaccct tagccacgcg gtaaatatgg cgggattaat tcaggctacc gcccactggt
     5221 tactgacaga acgcccgttc aaacatcagg agaaagatta cctgctgtat aaattcaacc
     5281 gtttccaggc ctgtcgctat gggctggaag gcgtcattac cgatccgcac actggcgatc
     5341 gtcgaccgct aacggaagac accttgcgat tgctggaaaa aatcgcccct tccgcacata
     5401 aaattggtgc atcgagcgcg attgaggccc tgcatcgcca ggtcgtcagc ggtctgaatg
     5461 aagcgcagct gatgcgcgat ttcgtcgccg atggtggctc gctgattggg ctggtgaaaa
     5521 agcattgtga gatctgggcc gatgactaag tagggaattg ccttacagaa gaatatccct
     5581 gacaatggtg ttttaactta catttaacaa tgttatgaaa caccctttag aaaccttgac
     5641 taccgcagca ggcattttgc tgatggcttt cctctcttgc ctgctgctgc ccgcccccgc
     5701 actggggctg acgctggcac aaaaactggt gaccatgttc catctgatgg atcttagcta
     5761 gctttacact ttattgtttt gtctgtggtt tttagtgctg ggcgctattg agtattttgt
     5821 tctgcgcttt atctggcgac gctggttctc gctggcggat taaacgtcga ttatcgccgt
     5881 acttcgcggc atactttgct tattctcctt cgcctaaagg aacgtttatg gataagcaat
     5941 cactgcacga aacggcgaaa cgcctggccc ttgagttacc ctttgtcgag ctttgctggc
     6001 cttttggccc ggagttcgat gtttttaaaa ttggcggcaa aatttttatg ctgtcgtcgg
     6061 agctgcgcgg cgtccccttt atcaatctga agtccgatcc acaaaaatcc ctgttaaatc
     6121 agcagatata cccgagcatt aagccagggt atcacatgaa taaaaagcac tggatttcgg
     6181 tgtatcccgg cgaggaaatc tccgaagcgt tacttcgcga tctgatcaac gattcgtgga
     6241 atctggtggt tgatggtctg gctaaacgcg atcaaaaaag agtgcgtcca ggctaaagcg
     6301 gaaatctata gcgcattttt ctcgcttacc atttctcgtt gaaccttgta atctgctggc
     6361 acgcaaaatt actttcacat ggagtcttta tggatatcat ttctgtcgcc ttaaagcgtc
     6421 attccactaa ggcatttgat gccagtaaaa aacttacccc ggaacaggcc gagcagatca
     6481 aaactctcct gcaatacagc ccatccagca ccaactccca gccgtggcat tttattgttg
     6541 ccagcacgga agaaggtaaa gcgcgtgttg ccaaatccgc tgccggtaat tatgtgttca
     6601 acgaacgtaa aatacttgat gcctcgcacg tcgtggtgtt ctgtgcaaaa accgcgatgg
     6661 acgatgcctg gctgaagctg gttgttgacc aggaagatgc tgatggccgc tttgccacgc
     6721 cggaagcgaa agccgcgaac gataaaggtc gcaagttctt cgctgatatg caccgtaaag
     6781 atctgcatga tgatgcagag tggatggcaa aacaggttta tctcaacgtc ggtaacttcc
     6841 tgctcggcgt ggcggctctg ggtctggacg cggtacccat cgaaggtttt gacgccgcca
     6901 tcctcgatgc agaatttggt ctgaaagaga aaggctacac cagtctggtg gttgttccgg
     6961 taggtcatca cagcgttgaa gattttaacg ctacgctgcc gaaatctcgt ctgccgcaaa
     7021 acatcacctt aaccgaagtg taattctctc ttgccgggca tctgcccggc tatttcccct
     7081 cagattctcc tgatttgcat aaccctgttt cagccgtcat cataggctgc tgttgtataa
     7141 aggagacgtt atgcaggatt taatatccca ggttgaagat ttagcgggta ttgagatcga
     7201 tcacaccacc tcgatggtga tgattttcgg tattattttt ctgaccgccg tcgtggtgca
     7261 tattattttg cattgggtgg tactgcggac cttcgaaaaa cgagccatcg ccagttcacg
     7321 gctttggttg caaatcataa cccagaataa actcttccac cgtttagctt ttaccctgca
     7381 ggggattatc gtcaatattc aggcggtatt ctggctgcaa aaaggcaccg aagcggcaga
     7441 tattctgact acctgcgcgc agttgtggat catgatgtat gcgctgcttt cagtcttctc
     7501 gttgctggat gttattttga atctggcgca gaaattcccg gcggcatctc agttaccgct
     7561 gaaagggata tttcagggga ttaaactgat cggcgcgatc ctggtcggca ttttgatgat
     7621 ctcgctgctg attggtcagt cgccagcgat tctgatcagc ggtcttggtg caatggctgc
     7681 cgtgctgatg ttggtattta aagatccgat tcttggtctg gtggccggta ttcagctttc
     7741 cgcgaacgat atgctgaaac tgggcgactg gctggagatg ccgaaatacg gcgcagatgg
     7801 cgcggtgatc gatattgggt taaccaccgt caaagtgcgt aactgggaca ataccattac
     7861 cactattccc acctggtctc tggtttctga ctcctttaaa aactggagcg ggatgtcagc
     7921 atctggcggg cgacgtatta agcgcagtat cagtattgat gtcaccagta ttcgttttct
     7981 tgatgaagac gaaatgcaac gtctgaataa agcgcatttg ttaaagcctt atttaaccag
     8041 ccgccatcag gaaattaatg agtggaatcg ccagcaaggt tctacggagt cggtattaaa
     8101 cctgcgccga atgaccaata ttggaacctt tcgtgcctat ctgaacgaat atctgcgtaa
     8161 ccatccgcgg attcgtaaag atatgacctt aatggtacgc caactggctc caggtgataa
     8221 cggtttaccg ctcgagatct acgcgtttac taacaccgtg gtgtggctgg aatatgaaag
     8281 cattcaggct gatatattcg atcacatatt tgcgattgtc gaagagtttg gtctgcgact
     8341 tcatcagtcg ccaaccggca atgatattcg ctctctggcg ggtgcattta agcagtaatt
     8401 aaaaaaaccg ctctcatcga atggatgaga gcggacatcg ttttgtcgga tgtagcgtga
     8461 atgccttatt tccgacgcag cgttttaaat gccacaaaca ggaatacaat ccacaccggc
     8521 agcaggatcg ctgacaagcg catatcatcc atcgtgcaca tcagcagcaa aatcatgccg
     8581 aggaaggcaa tgcagagata gtttccgaac ggatagagca gcgccttaaa ctgtgtttca
     8641 cgcccctgac gtcgcatcgc tgcacgaaaa cgcagatgcg ccagacagat cataatccag
     8701 ttcaacagca gcgttgctac caccagcgcc atcagcagac caaacgcttt ttgcggcagc
     8761 agatagttga ttaacaccac cagcgaagtg atcgctccgg aaagcatcag cgagttaatc
     8821 ggcacaccgc gacggctgac gcgagtcaaa aacttcggcg cattaccctg cacagaaagg
     8881 ccaaacagca tgcggctgtt agagtaaacc ccgctgttat acactgacag cgatgctacc
     8941 agaatgacga agttcagcgc agaagctacc acgttgctgt cgagattatg gaaaatcatc
     9001 acaaacgggc tactgttgga tttcacttcc acccacggat agagcgccag taaaaccacc
     9061 agtgaaccga tgtaaaacag caggatgcga tacaccacct gatttaccgc ttttggaatg
     9121 cttttatccg gatcgcgcgc ttcagcggca gtaatcccaa tcagctccag accgccgaag
     9181 gagaacataa ttaccgccag cgacaaaatc agcccattcc agccggtggc gaagaaacca
     9241 ccgtagcgcc agaggttgtc gatactggct ttctcgccgc cgtgaccaga aaacagcagc
     9301 cacaggccaa agccgatcat accgatgatt gccagcactt taatcaacgc aaaccagaac
     9361 tcggtttcgc catataagcg cacgttcacc aggttaacgg cgttgatgat aataaagaag
     9421 gcggcagccc aaatccacgt tggaacatcc gggaaccagt actgcatata gatgcccgca
     9481 gcggtcagct ctgccattcc caccagcacg aacattaccc agtagttcca gccagagagg
     9541 aagcccgcaa acggtcccca gtatttatag gcaaagtggg caaatgaacc ggataccggc
     9601 tcttcgacca ccatctcgcc aagctggcgc ataatcagga aagcgatgat cccggcgacg
     9661 ccgtaaccca gcaatacagc cggacccgcc atctgaatcg ccggaccaat gccaagaaac
     9721 agaccagtac caattgcgcc acccagcgca atcagttgaa tatgacggtt atgtaatccg
     9781 cgatgaagcg tcggctcttg attcgacgca gtatcttccg atacggttga cgcgtttttc
     9841 acgcctttcc cctgtgtgtc ttttttgttg aggggcacct tttaacattt agtgcccatc
     9901 gtcgcaagac acaatccaca cggttaaacg gggtatcctg cttttatttc cgtacccgat
     9961 gtcggtgcag ccacatcagc ttatacgccg ccgggataat aaacagcgac agcaaaggtg
    10021 cggtgatcat gccgccaatc atcggtgcgg caatccggct catcacctct gaaccagccc
    10081 ccgttcccca cagaatcggc agcagaccgg cgataatcac cgccaccgtc atcgctttcg
    10141 ggcgcacgcg cagtaccgcg ccgtgataca acgcctcatc cagcttctgc tcactgaatg
    10201 tttgcggatt attcaacgac ggctcggcct ctatggcgtg acgtaaatac atcagcatca
    10261 ccacgccaaa ttcggcggcg accccggcaa gggcaataaa accggtgccc gttgccacag
    10321 aaagatgaaa gcccatccac cacagcaacc agatgccgcc caccagcgca aacggtacgc
    10381 tgctgataat cagcaacgct tcgcccaccc gacggaatgc cagatacaac agcacgaaga
    10441 tgatcatcag tgtcatcggc accatgagtt tcagcttatg gttggcgcgc tccagtagct
    10501 cgaactgccc ggagaatgcc acgctggtgc ccggttttaa ctgcactttt tcagctatcg
    10561 ctttttgcaa atcgtgaacc accgacacca tgtcacgatc gcgggcatca atataaatcc
    10621 agctcgtcgg gcgcgcattc tcggttttca gcatcgacgg tccggtagag actttaacgt
    10681 cggccacgtc tgccagggtg atttgctgct tcatcggcgt caggatcggt agctggcgca
    10741 gtgcctgcgg gctatcgcgc cagctttgcg gataacgcag attaatggga taacgggcaa
    10801 tcccttccac cgtttcgcca accatcgccc cgcccaccgc agaagtcaca aacaactgca
    10861 catccgccac cgtcataccg taacgcgcgg ctttttcacg gttaatctca acgttgatat
    10921 agcgcccacc ttccagacgc tcggctagag cagaagccac gcttggcacc gttcgcgcta
    10981 cttcttcaat ttgctcagcc atcgcgtcga tatccgccag cacagtgccg gaaactttaa
    11041 tgccgatggg gcttttaatg ccggttgaga gcatatcgat acggttacga attggcggca
    11101 cccacagatt cgccagcccc ggcagccgca cggtgttatc cagttcctca atgattttgt
    11161 ccatcgtcat gccaggccgc cactgatcct gcggcttaag ctggatggtc gtttcaacca
    11221 tctcgagcgg agcagaatcg gtggcggttt ccgctttccc ggttttgcca aatacccgcg
    11281 ccacttcagg tacgctcata attagcttgt cggttttttg cagcatactc gccgcctctg
    11341 ctgcggaaat ccccggcagc gtcgatggca tatacaacaa gtcgccttca ttgatctgcg
    11401 gtaaaaattc cccgccaact ttattgagcg gccagagaac cgtcagcacc gaaagcgctg
    11461 ccaccaacag cgtggttttc ggccagtgca gtactttcag caatagagga tgataaacac
    11521 gaatcaaaaa gcgattgagc gggttactgc tttccggcgg aattttgcca cggatccagt
    11581 accccatcag gatagggatc actacgatcg ccagcagcgc cgcgcccgcc atcgcatagg
    11641 ttttagtgaa cgccaacggg ccaaacagac gcccttcctg cccttccagg gtgaagatcg
    11701 ggataaacga caacgtgata atcagcagac tgataaatag cgccggaccc acttcaacag
    11761 atgcatcggt gatcacctgc cagcgcgttt tattatccag cgtggcgtca ggatgctggt
    11821 gctgccactc ttccagccgt ttatgtgcat tttcgatcat gacgatagcg gcatcgacca
    11881 tcgccccgac ggcaatcgca atgccgccca acgacataat attggcattc agtccctgga
    11941 agtgcatgac aataaaagca atacacaacc ccagcggcaa cgaaataatc gccaccagcg
    12001 ccgagcgcac atgccagaga aacagcgcac agactaccgc caccacgata aactcttcca
    12061 gcaacttgcc gctgaggttg tcgatggcgc ggtcaatgag ctggctgcga tcgtatgttg
    12121 taactatctc cacgccttcc ggcagactac ttttcagcgt ttccagtttg tccttcacgg
    12181 aggcgatcac ttctcgggcg tttttgccgg agcgcaggat caccaccccg cccgccactt
    12241 cgccttcgcc attcagttcg gcaatgcccc ggcgcatctc cgggccaatc tggaccttcg
    12301 caacatcacg cagataaacg ggcacgccat tttctctggc ttttaaaacg atgtgattaa
    12361 agtcgtcgag cgtttgcaga tagccgctgg cgcgcaccat atattccgct tccgccagtt
    12421 cgatcgacga accgcccgct tcctggtttg aagcatccag cgcgcttttt acttcggcta
    12481 gactgatgcc atattgcgcc aggcgctggg gatcgataac cacctgatac tctttcacca
    12541 cgccgcccac cgacgccact tccgcaacgt cagggatggt ttttagctca tatttgagaa
    12601 accagtcctg caatgagcgt aaatcggcca gatcgtgctt accgctgcga tccaccagtg
    12661 catattcata gatccagcca acacccgtgg catctggtcc cagctcggcg ctgactcccg
    12721 caggcagctt accctgtacc tggttgaggt actccagcac ccgcgaacgc gcccagtacg
    12781 gatcggtacc atcttcgaaa atgacataca cataagagtc tccaaactgc gagaaaccgc
    12841 gcacagtctt cgcgccaggc accgacaaca tggtggtggt tagcggataa gtcacctgat
    12901 tttcaacgat ttgcggtgcc tgaccgggat agctggtttt aataatcacc tgcacatcgg
    12961 agagatccgg cagcgcatcc actggcgtgt taatgatggt ccaggtgccc cagatgctca
    13021 gaaacaacgc gcccatcagc accaaaaaac ggttcgccac cgagcgacga ataatccatt
    13081 caatcattgg ttattccctc aatgcgcatt ggtagcactt tcagagcgca tccgctccag
    13141 tgcgccagaa atattggctt cggaatcaat caggaacagg ccgctggaaa ccaccttttc
    13201 accttccgcc agaccagagt gtaacgcggt gacgccttgc gatgcctgga aaacggcaac
    13261 gcgtttcggt acaaagcgtc cgtcggcatc aacggtaatc acccgctgtt cgttgccggt
    13321 atcaatcagc gcctgtgacg gaatgagcag catcggttcg ctggcggtgt tgagttgcaa
    13381 ccaggcgttc atgcccggtt ttagcgcctc gtcggcgttg tcgacttcca gacgcagctg
    13441 cagcgtgcgg gtcgcggcat ccacgccagg tagcagcgtc catttgcgga tggtgagtgt
    13501 tttatccggt cgcgccggaa cggtgagcgt aaactgcgag gcatctttca ccagccaggc
    13561 gatagactcc gggatcgcag cagtgaccca caccgggtcc ataccctgaa ttttcgctac
    13621 cacgttatct ttggcgatat tcattcccgc gcgcagatca aacgcggtga tcacgccatc
    13681 aatgggtgct ttgagcgtaa agcgagtctg gattttttgc gtggcgatca ggcggcgaat
    13741 atccgcctcc ggcattcccg ccagtcgcag ccgctcaaga atgccttcag tctgggtcgc
    13801 cgtaccgccg gtttcgcgca gcagtaaata ctcactctgc gcttccaccc agtcaggaat
    13861 ggtcaggtcg agaagcggtg tgcccttttg tactttatcg cccacggtaa gcggatacac
    13921 cttgtcgata aacccggcag cgcgagcctg cacaatggca tactgatact cgttgtaact
    13981 gacattcgcc gggaaactct gggcaaaagt cagcggtccg cgcgtgacgg tagccgtttt
    14041 cacccccaga ttctgcgtct gagtcgggtc aatgcgcaca ccagacgcag aactctcttc
    14101 atcggcatat ttcggcacca gatccatatc cataaacggc gatttacctg gtttatcgaa
    14161 ccgcgtattg gggtacatcg ggtcgtacca gaataagatt ttacgttctg cggtcgacgt
    14221 tttttctgcg ggtggttcag cctttgcaaa ccaggtaaaa cctgccgcag aaataatacc
    14281 gcccgcgatc atgctgccga taataagcgc gatttttttc atctcattaa acctgggtta
    14341 ctggctgact ttaatatcct gtaataaaga aaggttgccc tgctggacaa aattaaacgc
    14401 cactttgtcg ccggttttaa tttcactcat tttcgtctgc ggggtgatgg taaagcgcat
    14461 agtcatctcc ggccagttca cggcagcaat cggatcgtga tggatggtga tttttttgct
    14521 ttccagatca atacccttta ccacgccagt ggcgctaata acctgcggtt gtacttcgct
    14581 catggtttca tgatgatgtt cgttagcctg ggcattaaag ccaataacgg taaacagact
    14641 gaacattgcg acttgcagtg cttttttcat ttattctctc ctggagttaa aaattattgc
    14701 tgccaaccgc cccctaaggc ggtatacaaa gaaatttcgt taacctgacg ggcataattc
    14761 aggtcgagta aagtttgtcg ggttgcaaat aaagaacgct cggcatccag cacttccaga
    14821 taacttaccg cgccgtgctg atataatgcc cgcgcccgtt gcaaagtaat ttgcagcgac
    14881 gccagataac gctgctgggc gctgatttga tcgttcaggc tttgacgtaa tgccagcgca
    14941 tctgccactt ctttaaaggc gttctggatt ttctgttcat aattcactac cgactgctgc
    15001 tggcgaattt cggcgatatc cagattggcc tggttgcgtc cggcattaaa aatggggatc
    15061 tcaattttag gaataaaatt ccacatcccg ctgctagcgt taaataatga agacagatcg
    15121 ctgctggcgg ttgatattcc gctggtcagg ctgatagacg ggaaaaatgc cgcacgcgca
    15181 gcaccaatat tagcattagc cgccattaac gcgtgttcag cttccataat atccgggcgc
    15241 tgcaataaga tttgcgacga caagcccgcc ggtaatttaa cgctttgcag gctgccgctg
    15301 tttactgtct gcgcttgcgg cagtttgccg tagcttccca ataacagttg caatgcatta
    15361 ttcgcctgcg ccagttcccc ctgacgttta gcgatgtcac tgcgggtact ttctatcacc
    15421 ccgcgagcct gttccagcgc cagaacattg ctgctaccgg tcaacagttg tttttcgaca
    15481 aacgcatatg actgctgata attacgcagc gtttcttcgg ctatttgcaa ttgcgcatac
    15541 gccagttgct gattgaaata gctttgcgcg acattagaaa ccagcagaat atgcaccgcg
    15601 cgctgagctt cctcagtggc taaataattt tgtcgctcgg cttcgctcat gttctttaag
    15661 cgaccgaaaa aatcgaggtc aaagctggcg ttaaggccgg tcgaaaactc ccgtgtcgtg
    15721 gcggtattcc ctttaagatt accgctccag ctgccgctgc cctcgccatt gagctgtggg
    15781 tagcggtcgg catcggtcag acgatattgc gcccgtgcct cctgcacttt cagcgtcgcc
    15841 atgcgcaaat cccggttatt caccagcgcc tcgctaatca gcgtcttcac ctgattatca
    15901 acaaaaaagg tgcgccagcc cacgttctgg tagttatctg ctgcgttaac caagccgttc
    15961 tggctgagtg agaactgctg cggcacgggc attgccgtac gctgataatc cggtgccagt
    16021 gaacaaccgg ttagcgcaag ggccacacaa aatggcagaa gtttacaagg agacataggc
    16081 tcataatttc tggtgatttt ataccgccaa ctttactcgc caggctctga ttttccggtg
    16141 acaggaaaat gacaaaattg tcattttgcc aataagcgat tgccatctga tcccgctact
    16201 ctagaattgc ccgggcaaca tgcggaggaa aaatgaaact gttgattgtc gaagatgaaa
    16261 agaaaaccgg agaatacttg accaaagggt taaccgaagc cggttttgtg gtcgatttgg
    16321 ccgacaacgg gctgaatggc taccatctgg cgatgaccgg tgattatgac ctgataatcc
    16381 tcgatattat gctgccggac gtgaacggct gggatatcgt gcgcatgttg cgctccgcca
    16441 ataaagggat gccgattctg ttgcttaccg cgcttggcac cattgaacat cgcgtcaagg
    16501 ggctggagtt gggggccgat gactatctgg tgaagccgtt cgcttttgct gaactgctgg
    16561 cgcgggtgcg cactctcctg cggcgcgggg cggcggtgat tatcgaaagt cagtttcagg
    16621 ttgccgatct gatggtcgat ctcgtcagcc gcaaagtcac ccgcagcggc acgcgcatca
    16681 ctctgaccag taaagagttt actctgctgg agttttttct ccgccatcag ggcgaagtgc
    16741 tgccccgctc gcttatcgcc tcgcaggtgt gggacatgaa tttcgacagc gacactaacg
    16801 ccattgatgt ggcggtgaag cggctacgcg gcaaaatcga caacgacttt gagccgaagc
    16861 tgattcagac cgtgcgcggc gtgggctata tgcttgaggt gccggatggt cagtaagcca
    16921 tttcagcgcc cgttttcgct ggcaacccgt ctgacctttt ttatcagcct ggccaccatc
    16981 gcggcgtttt tcgcctttgc atggatcatg atccactcag tgaaagtgca ttttgccgag
    17041 caggatatta atgatttaaa agaaattagc gccacccttg aacgggtact taatcatcct
    17101 gacgagacgc aagcccgacg cttaatgacg ctggaagata tcgtcagtgg ttattccaac
    17161 gtgttgattt ccctggcaga tagccacggc aaaacggtgt atcactcccc cggtgcgccg
    17221 gatatccgcg agtttacgcg tgacgccata cccgataaag acgctcaggg tggcgaggtg
    17281 tatctccttt ccggcccgac gattatgatg ccaggccacg gtcacgggca tatggaacac
    17341 agcaactggc ggatgattaa cttgccggtt ggcccgttgg tggacggcaa accgatttat
    17401 acgctctaca tcgcgctttc gatcgatttt catcttcatt acataaatga tttgatgaat
    17461 aaacttatta tgaccgcatc tgtcatcagc atcctgatcg tctttatcgt gctattggcg
    17521 gtacataaag gtcacgcgcc gatccgcagc gtcagccgtc aaatccagaa tatcacctcg
    17581 aaagatctcg acgttcgcct tgacccgcag accgagccca ttgagctgga acagctggta
    17641 ctgtcgttca accatatgat cgagcgtatt gaggatgtct ttacccgcca gtccaatttc
    17701 tcagcggata tcgctcacga aattcgcacg ccgattacca atctcattac gcaaacggaa
    17761 atcgccctca gccagtctcg cagccagaag gagctggaag atgtgctcta ctctaatctc
    17821 gaagagctga cgcgaatggc gaaaatggtc agcgatatgc tgtttctcgc tcaggccgat
    17881 aacaaccagc taatccccga aaagaaaatg ctcaacctgg cggatgaagt cggcaaagtg
    17941 ttcgattttt tcgaggcgtt agcggaagat cgcggcgtgg agttgcgatt tgttggcgac
    18001 aaatgtcagg ttgcgggcga tccgctgatg ctgcgtcggg cgttaagcaa cctgctttct
    18061 aatgccctgc gttatacgcc aaccagagag acaattgtag tgcgctgtca gacggtcgat
    18121 caccaggtgc aagttaccgt cgaaaacccc ggtacgccca ttgcgcccga gcacttaccg
    18181 cgattgtttg accgtttcta ccgcgttgac ccttcccgcc agcgaaaagg cgaaggtagc
    18241 ggcattgggc tggcgatagt gaaatcgatt gttgtcgcgc ataaaggcac ggttgcagta
    18301 acgtcagatg cgcgggggac aaggtttgtg attatgctgc cggagagaga gtgattatta
    18361 agttcaaatt cagggcgtgg tgcatcacct cctggtattg ttgatcaaca aattagagaa
    18421 aaacttatta aacatatgaa atatcgaagg cttttatttt tcaaagtttc acgagatgtt
    18481 tgtatatgaa acttagttaa atctgtgttt cttccaattg tagatcacaa tgccctttct
    18541 aaattttcat cgttataaac aagccatcta tcatataaag caatgaataa tttaatcgcg
    18601 attaatcatc attaattgtt atattattca ctcaatct
//
LOCUS       DAZOYL010000054        18523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZOYL010000054 DAZOYL010000000
VERSION     DAZOYL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.054"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHUOY_001366"
     CDS             <1..1710
                     /locus_tag="ACHUOY_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR0682187.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHUOY_001367"
     CDS             1711..3360
                     /locus_tag="ACHUOY_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR0682188.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHUOY_001368"
     CDS             3365..4141
                     /locus_tag="ACHUOY_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR0682189.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUOY_001369"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUOY_001369"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR0682190.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHUOY_001370"
     CDS             complement(5430..6752)
                     /locus_tag="ACHUOY_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR0682191.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUOY_001371"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUOY_001371"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR0682192.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGTVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUOY_001372"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUOY_001372"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR0682193.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUOY_001373"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUOY_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR0682194.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="ACHUOY_001374"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="ACHUOY_001374"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR0682195.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="ACHUOY_001375"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="ACHUOY_001375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR0682196.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="ACHUOY_001376"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="ACHUOY_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR0682197.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="ACHUOY_001377"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="ACHUOY_001377"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR0682198.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4525 a         4536 c         4866 g         4596 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaacccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgt gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAZOYL010000055        18188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZOYL010000055 DAZOYL010000000
VERSION     DAZOYL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.055"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(423..1361)
                     /gene="lrhA"
                     /locus_tag="ACHUOY_001378"
     CDS             complement(423..1361)
                     /gene="lrhA"
                     /locus_tag="ACHUOY_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR0682199.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            2276..3493
                     /gene="alaA"
                     /locus_tag="ACHUOY_001379"
     CDS             2276..3493
                     /gene="alaA"
                     /locus_tag="ACHUOY_001379"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR0682200.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            3577..4176
                     /gene="yfbR"
                     /locus_tag="ACHUOY_001380"
     CDS             3577..4176
                     /gene="yfbR"
                     /locus_tag="ACHUOY_001380"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR0682201.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(4235..6067)
                     /locus_tag="ACHUOY_001381"
     CDS             complement(4235..6067)
                     /locus_tag="ACHUOY_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0682202.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(6154..6802)
                     /gene="hxpA"
                     /locus_tag="ACHUOY_001382"
                     /pseudo
     CDS             complement(6154..6802)
                     /gene="hxpA"
                     /locus_tag="ACHUOY_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(6813..7307)
                     /locus_tag="ACHUOY_001383"
     CDS             complement(6813..7307)
                     /locus_tag="ACHUOY_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR0682203.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(7390..7845)
                     /gene="yfbV"
                     /locus_tag="ACHUOY_001384"
     CDS             complement(7390..7845)
                     /gene="yfbV"
                     /locus_tag="ACHUOY_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR0682204.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            8182..9384
                     /gene="ackA"
                     /locus_tag="ACHUOY_001385"
     CDS             8182..9384
                     /gene="ackA"
                     /locus_tag="ACHUOY_001385"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR0682205.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            9459..11603
                     /gene="pta"
                     /locus_tag="ACHUOY_001386"
     CDS             9459..11603
                     /gene="pta"
                     /locus_tag="ACHUOY_001386"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0682206.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            11793..13313
                     /gene="yfcC"
                     /locus_tag="ACHUOY_001387"
     CDS             11793..13313
                     /gene="yfcC"
                     /locus_tag="ACHUOY_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR0682207.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(13346..13888)
                     /gene="yfcD"
                     /locus_tag="ACHUOY_001388"
     CDS             complement(13346..13888)
                     /gene="yfcD"
                     /locus_tag="ACHUOY_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR0682208.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(13946..14497)
                     /gene="yfcE"
                     /locus_tag="ACHUOY_001389"
     CDS             complement(13946..14497)
                     /gene="yfcE"
                     /locus_tag="ACHUOY_001389"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR0682209.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(14553..15197)
                     /gene="yfcF"
                     /locus_tag="ACHUOY_001390"
     CDS             complement(14553..15197)
                     /gene="yfcF"
                     /locus_tag="ACHUOY_001390"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR0682210.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            15333..15980
                     /gene="yfcG"
                     /locus_tag="ACHUOY_001391"
     CDS             15333..15980
                     /gene="yfcG"
                     /locus_tag="ACHUOY_001391"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR0682211.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            16037..16399
                     /gene="folX"
                     /locus_tag="ACHUOY_001392"
     CDS             16037..16399
                     /gene="folX"
                     /locus_tag="ACHUOY_001392"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR0682212.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            16420..17313
                     /locus_tag="ACHUOY_001393"
     CDS             16420..17313
                     /locus_tag="ACHUOY_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR0682213.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(17361..>18188)
                     /gene="rpnB"
                     /locus_tag="ACHUOY_001394"
     CDS             complement(17361..>18188)
                     /gene="rpnB"
                     /locus_tag="ACHUOY_001394"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR0682214.1"
                     /translation="DTARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLL
                     WSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFY
                     HGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQK
                     HIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQE
                     KEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         4597 a         4772 c         4383 g         4436 t
ORIGIN      
        1 ccattagcat ttaatgccct ttcacgccag atccttgacg actccaggat aattagatgt
       61 tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt
      121 tgtgtcgatt cctcccccca aaagagagta ttttcttgat ctgtgacacg cttttgtcat
      181 tccataacaa aaacgcagca acaaatttac gcatttttaa catcattgta gcaggtgatt
      241 tttttcaggc gattatttgt gcgttcggga cgtgaatctc tggtgattga aaaatgaaca
      301 gttttgtacg ttctgcacta tgcgaggaag gatttttact aaaaaaaagc cgctggggtt
      361 taaaacaccc ccagcggctc gtttttacac tattgtctca ggaattatct atcgtccgtc
      421 gattactcga tatccctttc aatcaacaat gaatcatccc cttccggagc agacattggg
      481 ctgtattgcc acggattatg gtagctctcc atcgcctgat agatcacctg ggccagctca
      541 ttattactgg acgggtcata gcacagcaaa tattcggtat caggtagcgg cggtaagcca
      601 tctactccac tcagcacacg caggtacggg ctcatcattt caatcggcct tgccgtcacg
      661 ccaagaccgg ctttcactgc cgcacgaacg gccggaagcg tcgaggcgac ataagccagt
      721 cgccatggaa tatctgcttt attaagcgtc gccagcacca tatcgcgaaa cggacttgga
      781 tcatccagca atacaagagg gatcggctcg cctttttgca atacgtattc cgctgcgcag
      841 taccagtgtg ttggcgaggt acgcaggttc aatgccttaa acgctgaagg acgatgggtg
      901 gttaccatca aatccacttc ctgggattcc agcatttctg ccatataggc attacgttta
      961 acgcggacat ccagcgctaa tttcggataa accgaactca cgcgatttaa caggaaaggt
     1021 aagatcgtat cggcagattc atctgaagca ccgatagtta acacgccctg aagattactg
     1081 aacattaatg atgagcaggc ctcatcatta aaacgcagta ttttcctggc gtaaccaaga
     1141 agttgaatgc catgttcagt taacagtttg ttgcgaccgt gacgagcgaa cagttctttc
     1201 ccaacgagtt gttccagacg ctgcatttgc tgacttacgg cggactgagt acgacacaca
     1261 gcggcagctg cggcagcaaa agtgttcaga tcggcaacag caacaaatgt tctcagcaga
     1321 tcgaggtcga ggttaattat cggacgattt gcacttatca tatattatca cttactggcg
     1381 gctcatactg agctggttaa tgctgtgcac acacaaacaa gcaattccat ttgtaatgtg
     1441 cctccctggc agtttcatcc ggaacccgga cgaaagtaaa aatgcatatg agttgcacta
     1501 aaaaagcgac tcacattgtt ccgttataat gcctgaagta gatcacagaa tatatcttca
     1561 gggatcgcat atctattaag ttactcactc ttttctattt atgacatgcg cgtgtttgta
     1621 taaatgtaaa tgtgagtcct tgttccactc tcgtgcagca tcgctagtca tacgcgaaca
     1681 cgtacaaaca gcaatggtga ggcggcatca agagcaggga tccgtcattt atgcgagcat
     1741 tttaccccaa aaacctttta tttataaggg tcattgcgaa ttatctgatg caaagttatg
     1801 ttaggcaaag taatcttcta ttattaataa gcacatcaaa acctttttga atattaaata
     1861 ataattaatt agcatcatcc tcattcatta attccgctta acaatagttt cacaccttgc
     1921 ccccattgcg acacctcgga aatcatcacg ctgttaaact ctccgtaatg cggaaatatc
     1981 atcatcgcgt ttcattagtg aattcttaaa tgaggcattt tcacacaatt atcttacaga
     2041 taaaaaaacc agacttgcaa ttaagaatca gaacaattca ctatataaca ttgcatgcaa
     2101 agcatatacg cctcattatt ttgtcattat taagttatta acagcacaat cgagccttcc
     2161 cctctggcaa aatcttattc tgcagacctt caaaacaccg tcctggggga gtacattgtt
     2221 ctaagctgac ttccacggca gggagtggcg ataacagcaa aaaaggtcaa gattcatgtc
     2281 ccccattgaa aaatccagca aattagagaa tgtctgttat gacatccgtg gtccggtgct
     2341 gaaagaagca aaacgcctgg aagaagaagg taacaaggta ctgaaactga acatcggcaa
     2401 cccagcccca ttcggttttg acgcgccaga tgaaatcctc gttgacgtga tacgcaacct
     2461 gcctaccgct caagggtatt gcgattccaa agggctttac tccgcgcgta aagccatcat
     2521 gcagcactac caggctcgtg gcatgcgtga tgttaccgtg gaagatattt acatcggcaa
     2581 tggtgtatcg gagcttatcg ttcaggcaat gcaggcattg ctgaacagcg gggacgaaat
     2641 gttggttcct gcaccagatt acccactctg gaccgcggcg gtttcgcttt ccagcggtaa
     2701 agcggtgcat tatctttgcg atgaatcctc tgactggttc ccggacctcg atgatattcg
     2761 cgctaaaatt acgcctcgta cgcgtgggat cgttattatc aacccaaata acccaaccgg
     2821 cgcggtatat tccaaagagc ttttaatgga gattgtggag attgcacgtc agcataatct
     2881 cattatcttc gccgatgaaa tttatgacaa aattctctac gacgatgctg agcatcactc
     2941 aattgcgcca ctggcacctg acctgctgac cattaccttt aacggactgt cgaaaacgta
     3001 ccgcgttgca ggcttccgtc aggggtggat ggtgttgaac gggccgaaaa aacacgccaa
     3061 aggctacatc gaaggtctgg aaatgctggc ttcaatgcgc ctgtgtgcta acgttcctgc
     3121 gcaacacgcc attcagaccg cgctgggtgg ttatcagagc atcagtgaat ttattacccc
     3181 tggcggtcgt ctttatgagc agcgtaaccg cgcgtgggaa ctgatcaacg atattccggg
     3241 cgtttcctgc gtgaaacctc gtggtgcgct gtatatgttc ccgaaaatcg acgccaaacg
     3301 ctttaacatt cacgacgatc agaaaatggt gctggatttc ctgttgcagg aaaaagttct
     3361 gttggtgcaa gggacggcat tcaactggcc gtggccggat cacttccgca ttgtcacgct
     3421 accgcgtgtc gatgatatcg agctgtcttt gagcaagttc gcgcgtttcc tttctggtta
     3481 tcatcagctg taatcttaat ttcactgccg gagattgcat ccggcagcgt tatcccgcca
     3541 caatgacctg atgatgtcat catacgtaag gtcactatga aacagagcca tttttttgcc
     3601 catctctccc gcctgaaact cattaaccgc tggccgctaa tgcgcaacgt gcggacggaa
     3661 aatgtgtccg aacacagttt gcaggtagcg atggtcgccc atgcgctggc agctatcaaa
     3721 aatcgaaaat ttggcggtaa tgtcaacgcc gaacgtatcg ctttactggc gatgtaccac
     3781 gatgcctcag aagtgctcac cggcgatctc cctactccgg tgaaatactt caattcgcaa
     3841 atcgctcagg agtacaaggc tattgaaaaa atcgctcagc aaaaactggt caatatggtt
     3901 ccggaagagc tgcgggatat ctttgcgccg ttaattgacg agcatgcata tagcgatgaa
     3961 gaaaaatcgc tggtgaaaca ggcagatgca ctgtgtgcat atctgaaatg tctggaagaa
     4021 ctcgcggccg gaaataatga attcttgctg gcaaaaacgc gactggaagc gacgcttgaa
     4081 gcgcgtcgca gccaggagat ggactacttc atggaagtat ttgttcccag cttccatctt
     4141 tcgctcgatg agattagcca ggattcaccg ctgtaagcag ccggagtctg cgtcgcatca
     4201 ggcaataagc gccggatgcg acatcaggct cttgtcaaaa cggaaacagc atcgggatca
     4261 tcaccacaca aaccgccatc acgataatgg tgaacggtac ccccaacttc acaaagtcac
     4321 tgaagctgta atttcccgga cctaaaacca gtgtgttaac aggtgaagaa accggtgtca
     4381 taaaggcggc ggatgctgcc atcgccacga ccatcgcgaa tggataaggc gacaccccca
     4441 tcgttttggc agcagccagc gcaatcggag ccatcaacac cgccgtcgcg gtattagaga
     4501 taaatagccc aataaccgcc gacaagacaa acaaacaccc cagcatcata tgtggcccgt
     4561 aaccaccgcc aatgtccatc agccctttca ccgccagcgc gacacctccc gttttctgta
     4621 atgccacagc aaacggcatc atcccaacga tcaaaataat gctcggccag tgaatggatt
     4681 tataggcgct ttcagcatct atacagcgga atttccccat cagcaggcag gcgatgatag
     4741 cggcaacagg attaggaatt tcatctgtca gcattaacgc caccattagc accagacaga
     4801 aaatggcatg gggtgcctgg ctgtgcgcgg gcgatgcttc actcacctct tccggtaagt
     4861 tcagcgctac gaagtcgcgg ccctgtttgg ccagcatacc gatcagtttc cagttaccca
     4921 caaccaggat gatatcgccc agcagcagag gctcatccgc cagcgaacct tccagcgcca
     4981 cgccattgcg ctttagcccc accacattca gtccgtagcg ggtacgaaaa ccaatttcgc
     5041 gcaccgattt accaatcagt tctgactcag gaattaatga aatctctgcc atgcccacat
     5101 caagggcctg gtcagaaaaa tactcgccgc gcagtaccat cggctccagc aattgctcac
     5161 tacaaaattg ccggagatcg acatcagccg cagacatatc aataagcaaa acgtcacgcg
     5221 cgcgaaattc agaaacccca ttaacgttca cgataacgcg acgaaaacgc cgccagcgtt
     5281 caacaccgat gacgttagcg ccataacgct cacgtaattt gagatcatcc agccgttgac
     5341 caatcattgg cgatccgggg cgaatagcca gacgtcgcgc acgcccggtc agtcgatatt
     5401 cacggataag atcgcgaaag gttcgacgcg tccagccttc gcgctgcggg gtctgggtat
     5461 cccctttcag catgaaacgc atcactaaca tatacaaaat acccagcacc agcacgacca
     5521 ggccaatagg tgttacgcta aagaaactga agccgtgata gccttcacgc agcaattcac
     5581 tgttgactac caggttcggc ggcgtcgcca ccagcgtcat catgccgctt atcagcccgg
     5641 caaaactcaa cggcatcatc agacgtgacg gcgaagtttg catacgcatg gcaacgctta
     5701 acaccacggg aataaagata gcgacaacgc cggttgaact cataaacgcg ccaagccccg
     5761 cgacggtcag catcaacaaa accaacattt tgatttcact gttgcccgca actttgacca
     5821 gccatgttcc cattacggtg gcaacaccgg tacggaccaa accatcgcca ataataaaca
     5881 aggcggcaat caggacaacg ttaggatcag aaaagccgga aaatacttcg gggactgtca
     5941 gcgttccgct taatgcaaac gcgacaataa caaacaaagc gaccgcatcc atacgcactc
     6001 tgcccgtcgc aaacaagaca atggcaacgg ccagtaatga aagaacccaa atcaattcac
     6061 cgttcacaac ttatccttgt taattgaggg agatgtcttg attctgccat aaaaaagccc
     6121 cgacgagacg gggctaaatc atgatcaggt gtttcactga ataataacat cgccatttgg
     6181 ctgtttggtc acagtaattt gctccagact gtggaggacc aaatcgacct cattcaggcg
     6241 cggggtatct gccggagcgt taaccgcaat gacatgacaa cccgccgcca ggccagaaag
     6301 cacgccagcg gagcatcttc caccaccaca cactcctgcg gcgcaagccc cagcagctgc
     6361 gcgcctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc agcggttaca
     6421 aacacctctg gtgcgggaag cccagctatt ttatggcgcg ctcgcgctac cggcatggag
     6481 ccagaagtca caatggccca cggaatacct gctttattca aatgactgag taaggcgatt
     6541 gcccccggaa gcgggtaata ccttcggttt ccgtggcctc gatgtgctcc agacgcgtaa
     6601 actcggcggc aatatcagcc tcggatttgc ccgccataaa atggcgcaga gaggtgatcg
     6661 cctgtttacc gtgaatgaaa gccagcacct cttccggcgc taacccatga cgtctggccc
     6721 agttgctcca cgcccgttct accgcaggca gggaatccac cagcgttcca tcaagatcaa
     6781 acagaaaacc tttgcaccgc acgcgggcct cctcaggcat tgataatttg attaatttcg
     6841 ttggcgctca aatggtactg acgcgggcag gcatgccaca cattaagcat gcgctgatat
     6901 ttttcccaca ttggcgtctg ggcgttaaaa ccgtgagttc cggcgtcaaa atgggtatag
     6961 cgcccttcca cattaaccat aaagcggaca taaccgaggt aacgtgcttc agtggcggcg
     7021 tcaaagccga ggaaggtgac acgacgttca tcgatggatt gctggtcctg caaattagac
     7081 caggaaacat gcaacgcatg atacatctcc ataatgtcga tgatagtgcg gcaggtttct
     7141 tctttcagct cgccaaactc gcgatccaat tcacgcatct gtaatccgta acctcgctca
     7201 ataattgttt gcaggcgacg gtaacgttca gcatttgccg gatcgagcat agtcatcatc
     7261 ttgtactgat tagacaaaat aagacgttgc gcgttggtca tttccattgt tgactcctgt
     7321 atcactctac tacggtgaaa aaaaagaagg ctgagtatgc cttcttttat atgcgtaatc
     7381 aggggtcaat tacaaatcat caaggaaagt tttatccagt tgtttgaagg cgcgcttaag
     7441 cgtgtcagct aatgcctggt aatcaggctt gccttcaacg ggtgccaaca cctgtccaga
     7501 ctcctgcaat ttaccgcgaa cttcataaaa ccagttaagg attgcagggg gtaatggcgt
     7561 tacagaacgc ttgcccagcc accacaatcc ctgcatgggt aaacttaagg cgaacagtgc
     7621 agtggcaact gccggcccaa gctgaccgcc cagggcaatc tgccagcaga gagtaaatac
     7681 ggcgatcggc ggcataaaac ggatcgcata acgcgtcatc ttgataacgc gattttcgac
     7741 aaagaccggg gcaaggcgtt tttccagtgg ccacgtcttt gagtaatgct gtccgcggcg
     7801 aaacaagcta aaaaaattaa cagaacgatt atccggcgtt gacatgcttc acctcaactt
     7861 cacatataaa gattcaaaaa tttgtgcaaa ttcacaactc agcgggacaa cgttcaaaac
     7921 attttgtctt ccatacccac tatcaggtat cctttagcag cctgaaggcc taagtagtac
     7981 atattcattg agtcgtcaaa ttcatataca ttatgccttg gctgaaaatt acgcaaaatg
     8041 gcatagactc aagatatttc ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat
     8101 cataaatgtc ggtgtcatca tgcgctacgc tctatggctc cctgacgttt ttttagccac
     8161 gtatcaatta taggtacttc catgtcgagt aagttagtac tggttctgaa ctgcggtagt
     8221 tcttcactga aatttgccat catcgatgca gtaaatggtg aagagtacct ttctggttta
     8281 gccgaatgtt tccacctgcc tgaagcacgt atcaaatgga aaatggacgg caataaacag
     8341 gaagcggctt taggtgcagg cgccgctcac agcgaagcgc tcaactttat cgttaatact
     8401 attctggcac aaaaaccaga actgtctgcg cagctgactg ctatcggtca ccgtatcgta
     8461 cacggcggcg aaaagtatac cagctccgta gtgatcgatg agtctgttat tcagggtatc
     8521 aaagatgcag cttcttttgc accgctgcac aacccggctc acctgatcgg tatcgaagaa
     8581 gctctgaaat ctttcccaca gctgaaagac aaaaacgttg ctgtatttga caccgcgttc
     8641 caccagacta tgccggaaga gtcttacctc tacgccctgc cttacaacct gtacaaagag
     8701 cacggcatcc gtcgttacgg cgcgcacggc accagccact tctatgtaac ccaggaagcg
     8761 gcaaaaatgc tgaacaaacc ggtagaagaa ctgaacatca tcacctgcca cctgggcaac
     8821 ggtggttccg tttctgctat ccgcaacggt aaatgcgttg acacctctat gggcctgacc
     8881 ccgctggaag gtctggtcat gggtacccgt tctggtgata tcgatccggc gatcatcttc
     8941 cacctgcacg acaccctggg catgagcgtt gacgcaatca acaaactgct gaccaaagag
     9001 tctggcctgc tgggtctgac cgaagtgacc agcgactgcc gctatgttga agacaactac
     9061 gcgacgaaag aagacgcgaa gcgcgcaatg gacgtttact gccaccgcct ggcgaaatac
     9121 atcggtgcct acactgcgct gatggatggt cgtctggacg ctgttgtatt cactggtggt
     9181 atcggtgaaa atgccgcgat ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc
     9241 tttgaagttg atcatgaacg caacctggct gcacgtttcg gcaaatctgg tttcatcaac
     9301 aaagaaggta cccgtcctgc ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa
     9361 gacgcgagcc gcctgactgc ctgatttcac accgccagct cagctggcgg tgctgttttg
     9421 taacccgcca aatcggcggt aacgaaagag gataaaccgt gtcccgtatt attatgctga
     9481 tccctaccgg aaccagcgtc ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg
     9541 aacgcaaagg cgttcgtctg agcgttttca aacctatcgc tcagccgcgt accggtggcg
     9601 atgcgcccga tcagactacg actatcgtgc gtgcgaactc ttccaccacg acggccgctg
     9661 aaccgctgaa aatgagctac gttgaaggtc tgctttccag caatcagaaa gatgtgctga
     9721 tggaagagat cgtcgcaaac taccacgcta acaccaaaga cgctgaagtc gttctggttg
     9781 aaggtctggt cccgacacgt aagcaccagt ttgcccagtc tctgaactac gaaatcgcta
     9841 aaacgctgaa tgcggaaatc gtcttcgtta tgtctcaggg cactgacacc ccggaacagc
     9901 tgaaagagcg tatcgaactg acccgcaaca gcttcggcgg tgccaaaaac accaacatca
     9961 ccggcgttat cgttaacaaa ctgaacgcac cggttgatga acagggtcgt actcgcccgg
    10021 atctgtccga gattttcgac gactcttcca aagctaaagt aaacaatgtt gatccggcga
    10081 agctgcaaga atccagccct ctgccggttc tcggcgctgt gccgtggagc tttgacctga
    10141 tcgcgactcg tgcgatcgat atggctcgcc acctgaatgc gaccatcatc aacgaaggcg
    10201 acatcaatac tcgccgcgtt aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc
    10261 tggagcactt ccgtgccggt tctctgctgg tgacttccgc agaccgtcct gacgtgctgg
    10321 tggccgcttg cctggcagcc atgaacggcg tagaaatcgg tgccctgctg ctgactggcg
    10381 gctacgaaat ggacgcgcgc atttctaaac tgtgcgaacg tgctttcgct accggcctgc
    10441 cggtatttat ggtgaacacc aacacctggc agacctctct gagcctgcag agcttcaacc
    10501 tggaagttcc ggttgacgat cacgaacgta tcgagaaagt tcaggaatac gttgctaact
    10561 acatcaacgc tgactggatc gaatctctga ctgccacttc tgagcgcagc cgtcgtctgt
    10621 ctccgcctgc gttccgttat cagctgactg aacttgcgcg caaagcgggc aaacgtatcg
    10681 tactgccgga aggtgacgaa ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg
    10741 gtatcgcaac ttgcgtactg ctgggtaatc cggcagagat caaccgtgtt gcagcgtctc
    10801 agggtgtaga actgggtgca gggattgaaa tcgttgatcc agaagtggtt cgcgaaagct
    10861 atgttggtcg tctggtcgaa ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg
    10921 aacagctgga agacaacgtg gtgctcggta cgctgatgct ggaacaggat gaagttgatg
    10981 gtctggtttc cggtgctgtt cacactaccg caaacaccat ccgtccgccg ctgcagctga
    11041 tcaaaactgc accgggcagc tccctggtat cttccgtgtt cttcatgctg ctgccggaac
    11101 aggtttacgt ttacggtgac tgtgcgatca acccggatcc gaccgctgaa cagctggcag
    11161 aaatcgcgat tcagtccgct gattccgctg cggccttcgg tatcgaaccg cgcgttgcta
    11221 tgctctccta ctccaccggt acttctggtg caggtagcga cgtagaaaaa gttcgcgaag
    11281 caactcgtct ggcgcaggaa aaacgtcctg acctgatgat cgacggtccg ctgcagtacg
    11341 acgctgcggt aatggctgac gttgcgaaat ccaaagcgcc gaactctccg gttgcaggtc
    11401 gcgctaccgt gtttatcttc ccggatctga acaccggtaa caccacctac aaagcggtac
    11461 agcgttctgc cgacctgatc tccatcgggc cgatgctgca gggtatgcgc aagccggtta
    11521 acgacctgtc ccgtggcgca ctggttgacg atatcgtcta caccatcgcg ctgactgcga
    11581 ttcagtctgc acagcagcag taacctcgtc atcatccgca gctttgcgct gcggatatct
    11641 gaaccggaaa taatcactat ttccggtttt ttattctctt aatctgcatt aatcctttct
    11701 gattatcttg cttaactgcg ctgcatcaat gaattgcgcc atttcacttt acatacttac
    11761 cactttgttt tgtgcaaggg aatatttgcg ctatgtccgc aatcactgaa tccaaaccaa
    11821 caagaagatg ggcaatgccc gatacgttgg tgattatctt ttttgttgct attttaacca
    11881 gccttgccac ctgggtagtt ccggtgggga tgtttgacag tcaggaagtg cagtatcagg
    11941 ttgatggtca aacaaaaaca cgcaaagtcg tagatccaca ctcatttcgc attctgacta
    12001 acgaagcagg cgaacctgag tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc
    12061 cgggcctgat gaacttcccg tttgaaggat taacctcagg atcgaaatac gggacagctg
    12121 ttggcatcat catgtttatg ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag
    12181 gaaccattga taacggtatc ctggcgctta ttcgccatac ccgcgggaat gaaattctct
    12241 ttattcctgc gctgtttatt ctgttttcac ttggcggcgc ggtatttggt atgggagaag
    12301 aggccgtcgc ctttgccatt atcatcgcac cgctaatggt ccggctgggc tatgacagta
    12361 ttaccaccgt cctggtgacc tatattgcca cgcaaatcgg ttttgccagt tcgtggatga
    12421 acccgttttg tgtggtcgtt gctcagggga ttgccggcgt tccggtgctt tctggctccg
    12481 ggttgcgcat cgtggtgtgg gttatcgcca ctatgattgg cctgatcttt accatggtgt
    12541 acgcctcacg agtgaaaaag aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct
    12601 ttcgtgaaaa gcaggcggat gttgaacaac gtccgtttac ctttggtgac tggctggtat
    12661 tgattgtcct gaccgccgta atggtctggg tgatttgggg cgtgatcgtt aatgcctggt
    12721 ttattccaga aattgccagc cagttcttca ccatgggtct ggtgattggc atcatcggcg
    12781 ttgttttccg ccttaacggc atgacggtta ataccatggc ttcatccttt accgaaggga
    12841 cgcgaatgat gatcgcccct gccctgctgg tgggtttcgc caaagggatt ttgctgctgg
    12901 tcggtaatgg tgaagcgggt gatgccagcg tgttaaatac catcctcaac agcattgcca
    12961 atgccattag cggtctggac aacgcggtcg cggcctggtt tatgttgctc ttccaggcgg
    13021 tatttaattt cttcgtgacg tccggttctg gtcaggcggc gttaaccatg ccgttactgg
    13081 caccgcttgg cgatctggtc ggtgttaacc gtcaggttac cgtgctggca ttccagtttg
    13141 gtgatggctt cagtcacatc atttacccaa cctcagcttc gttaatggca actctcggtg
    13201 tgtgcagggt ggacttccgt aactggctga aggtgggcgc gaccctactt ggactgctgt
    13261 ttattatgtc cagcgtcgta gtgatcggcg ctcagttgat gggctaccac taaaaatgtt
    13321 aagagccgca attgcggctc ttttttcatt ctgccgtttc agtctctacg gcttcatttt
    13381 tggcattgcg cttcatccac aacgccagcg ctttcagcga gtctggagtg aactcatcgc
    13441 agcgtgcggt gatttcttcc ggcgtcagcc agcaaacttc actgacttca tcttcctgta
    13501 gggcgaaggg accgtgagag acgcagctga acaacgcgcc ccagacacgg caatttttat
    13561 cttcgaaata gaactgcccg tgctcggcaa aggggacacc ggcaatgccc aactcttctt
    13621 ccgcttcgcg acgcgcggat tccagcagtt gctcatcggc ctgaaccaca ccgcctgcgg
    13681 tcgcatctaa catgccgggt aaaaagtctt ttgtctcggt acgacgctgg accagaattt
    13741 tgcccatgcc atcatgcacg acgatgtaag ttgcacgatg acgcagacac tgtgcccgca
    13801 tttgttcccg gctggcttgt gcaatgactt cgttctcttc attgacaata tccacccatt
    13861 cagtacttgc caaacgacgc tgttccatta tcaggaaacc ttctttttct ggcgctctta
    13921 cggcgcattt tgagttgtgg gtaaattacg gattaatcgc gacctgcgca atgatacttt
    13981 gatcattgag tgcgattaca ctaagtacgt cattatccag catgccataa ctcgccggat
    14041 taccgccttt cggaatactc accgagccgg ggttgaagtg gaaaatctcc ccccgctgtt
    14101 ctgccactgg cagatgggta tgaccgtaca ccagcacatc gttctggttt aaagcaggta
    14161 gattttccgg gccaaaaaga tgaccatgcg tcaaaaacag acgttgtttt tccagtaata
    14221 cctgttgcca cggcgcggtt atcgggaaat gcagcagcat ttgatccact tcgctgtcgc
    14281 agttgccgcg cacagcgata accttatgtg ccacttcatt aagccgttca gcgactttgg
    14341 ctggcgcgta accctccggt aaagcattac gcgggccgtg attcaacacg tcgccaagga
    14401 tcaccagcca ctgggcaccg ctttgggcaa acaactccag aacacgttcc gtcgccggta
    14461 acgacccatg aatgtccgat gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac
    14521 gtaagctatg atactggatt ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca
    14581 ataaaacgct ggacagacgc tcgctgccac tggaatgtcg cataatccac caggcgttct
    14641 ggcacctcat cgccatgtag taccaggcgg ttaatcatta gcgccagatc agtatcagca
    14701 atgcaccatt caccaaataa attcggctga cccagtgcta acaaatgttc tgccatcgcg
    14761 aacagtttct ctgcactggc ttttccctcg gccgttagtg gcgctttttt cgcccccgca
    14821 aagacaacat ccgtcggacg ctcttcgcgg atgggcatca gatcgctgcg cagccaggcc
    14881 tgaatccgtc gtgcacgcgc acgattttct aaatcaagcg gataaatacg ttcccaggtc
    14941 ggtggcgcaa atcgatcttc cagatactcc gcaatggcag aagattcact caactcaaaa
    15001 tcatcgattt gtaataacgg cacacggcgt gtctgaccgt aaccttgcca cgtcggcttc
    15061 aaatgttcac cgctgtcgag gtcgatggtc ttgatatgaa acgacaggcc tttttcctgc
    15121 aacgccaccc aggcggataa cacataaggg gagaaaaagt gggcatctga ccaaagcgtg
    15181 atagcgggtt tactcattat gtcctcggca ataaaggggg ctggctatca aactatagag
    15241 cctttttgtc actgtcacgt gacgaatact cacgtaagtc gccgcgcttt ctatacttgt
    15301 ggtgttcaga tgattaaaca accggagctg caatgatcga tctctatttc gccccgacac
    15361 ccaatggtca caaaattacg ctgtttctcg aagaagcaga gcttgattat cgcttgatta
    15421 aggtagacct ggggaaaggc ggtcagtttc gtccggaatt tttgcgcatt tcgcccaaca
    15481 acaaaattcc ggcaattgtt gatcattctc ctgccgatgg cggcgaaccg ctaagcctct
    15541 ttgagtctgg tgccattttg ttgtatctga ctgagaaaac aggactcttt ttgagtcatg
    15601 aaacgcgtga gcgcgccgcc acattacagt ggttattctg gcaggtaggc ggactggggc
    15661 cgatgcttgg gcaaaatcat cattttaatc acgcagcccc ccaaaccatt ccttacgcta
    15721 ttgaacgtta tcaggttgaa actcagcgtc tttaccatgt actaaacaag cggctggaaa
    15781 actcgccctg gctgggaggc gagaactaca gcattgcgga tattgcctgc tggccgtggg
    15841 ttaatgcctg gactcgccag cgaattgacc tcgcaatgta tccggcagtc aagaactggc
    15901 atgagcggat ccgttcgcgc cctgccaccg ggcaggcact gctaaaagca caactcggtg
    15961 atgagcgttc ggatagttaa cagaaacagg ttctcgtgta ttatttcatc ctaagtaaaa
    16021 caacggagaa cctgcaatgg cacaacctgc cgctattatt cgtataaaga accttcgttt
    16081 acgtacgttt atcggaatta aggaagaaga aattaacaac cgtcaggata ttgttatcaa
    16141 tgtgacgatc cactaccccg ccgataaagc gcgcactagc gaagatatca acgatgcgct
    16201 gaattatcgc accgtaacga aaaacattat tcagcatgta gagaataacc gtttctcttt
    16261 gttggaaaaa ttaactcagg atgtgctcga tatcgcacgt gaacatcact gggtgacgta
    16321 tgctgaagtg gagatcgata aactgcacgc gctgcgctac gccgattcgg tatccatgac
    16381 cttaagctgg cagcgttaac cgccatatcg ggaggctgca tgaacatagt gatcaccgga
    16441 gggacgggat taattggtcg ccatttgatt ccgcgtttgc tggagctggg ccatcaaatc
    16501 acggtagtga cgcgtaaccc acagaaagcc agttccgttc tcggccctcg ggtgacgcta
    16561 tggcaagggc tagccgatca aagcaacctc aacggcgttg atgcggtaat caacctggcc
    16621 ggagaaccga ttgctgataa acgctggact cacgagcaaa aagagcgtct ctgccaaagc
    16681 cgctggaata tcacgcaaaa actggtcgat ttgattaatg ccagcgacac gccgccgtcg
    16741 gtattcattt ccggctcggc aacgggctat tatggcgact taggtgaagt ggtggttacc
    16801 gaagaggaac cgccgcataa cgaatttact cataaactct gcgcccgctg ggaagagatt
    16861 gcctgccggg cgcaaagtga caaaacgcga gtgtgcctgc tgcgaaccgg cgtggtactg
    16921 gcaccggacg gcggtattct cggtaaaatg ctgccaccgt ttcgtcttgg cctgggcggg
    16981 ccgattggtt ccggtcggca gtatctggcc tggattcata ttgatgatat ggtcaacggc
    17041 attctctggc tgctggataa cgacctgcgc gggccattta atatggtttc gccctacccg
    17101 gtacgcaatg aacaatttgc ccatgcgctc ggtcaagcgc tgcatcgccc ggccattttg
    17161 cgcgtccccg caaccgccat tcggctgtta atgggcgaat cttcaatact ggtattaggt
    17221 ggacaacgcg cgctgcctaa acggctggaa gaagcgggtt ttgcgtttcg ctggtacgat
    17281 ttagaagagg cgctggcgga tgtcgttcgc tgatgtggtt tacagcaaac atccgccagt
    17341 taactcccgg tgttacagga ttagtggctt tgcgcgataa gatcgtctgg tgaaagtcgg
    17401 gtcaccatca taactaactc tctgtctaaa cctctatcca gcatctcctg agcaatacgc
    17461 agggcttctt cgtgtttgcc ctgcattgcg ccttcttcac gtaatctgtc agcaatggtc
    17521 atcagtttct ccttttcttg tggtgcgcgt tctgttatct caccaataaa tgcacggaaa
    17581 cgctgggcat cccctgtttg taatacgtaa ttaaacaggg cttttagctg tctgtcatta
    17641 gtgttccctg taactagcag cgaaacaatt tggtcgacta atcccaacag atcgcgctga
    17701 cgaatatgtt tctgaattaa ctccaacagc gccattttgc ggtgttgcat aatctcgtca
    17761 tccggcacca cggtaatatc caccaacgga aacgctgatg aatatatttt gcgggctata
    17821 gcaggctcgg caaattcatc aagccagcag agtgaataag gataaggact tctgcaacca
    17881 tgataaaaca gcatcgggat caccaatgga agctctttat agcccgcatc aagatggttt
    17941 tgcattgccg caatggaata acgcatcatg cgaaaagcca ttaattcttc cagcttactt
    18001 tggtgctcta tcactacata aatataaccc actccctcct gcgttttcac agaccacaag
    18061 aggtcggaat aatattgccg caggtcttca tcaataaaac tgtttgattc tagtttaagc
    18121 gtcgttaaat cacacagttt gcgcagcggc gcgggaagat gaatatcaat aaaatcccgc
    18181 gcggtgtc
//
LOCUS       DAZOYL010000056        18044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZOYL010000056 DAZOYL010000000
VERSION     DAZOYL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.056"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            317..995
                     /locus_tag="ACHUOY_001395"
                     /pseudo
     CDS             317..995
                     /locus_tag="ACHUOY_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1039..5889
                     /locus_tag="ACHUOY_001396"
     CDS             1039..5889
                     /locus_tag="ACHUOY_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR0682215.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWIYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6257..7912
                     /gene="lldP"
                     /locus_tag="ACHUOY_001397"
     CDS             6257..7912
                     /gene="lldP"
                     /locus_tag="ACHUOY_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR0682216.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7912..8688
                     /gene="lldR"
                     /locus_tag="ACHUOY_001398"
     CDS             7912..8688
                     /gene="lldR"
                     /locus_tag="ACHUOY_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR0682217.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8685..9875
                     /gene="lldD"
                     /locus_tag="ACHUOY_001399"
     CDS             8685..9875
                     /gene="lldD"
                     /locus_tag="ACHUOY_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR0682218.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10071..10544
                     /gene="trmL"
                     /locus_tag="ACHUOY_001400"
     CDS             10071..10544
                     /gene="trmL"
                     /locus_tag="ACHUOY_001400"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR0682219.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10597..11418)
                     /gene="cysE"
                     /locus_tag="ACHUOY_001401"
     CDS             complement(10597..11418)
                     /gene="cysE"
                     /locus_tag="ACHUOY_001401"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR0682220.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11498..12517)
                     /gene="gpsA"
                     /locus_tag="ACHUOY_001402"
     CDS             complement(11498..12517)
                     /gene="gpsA"
                     /locus_tag="ACHUOY_001402"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR0682221.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12517..12984)
                     /gene="secB"
                     /locus_tag="ACHUOY_001403"
     CDS             complement(12517..12984)
                     /gene="secB"
                     /locus_tag="ACHUOY_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR0682222.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(13047..13298)
                     /gene="grxC"
                     /locus_tag="ACHUOY_001404"
     CDS             complement(13047..13298)
                     /gene="grxC"
                     /locus_tag="ACHUOY_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR0682223.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13440..13871)
                     /locus_tag="ACHUOY_001405"
     CDS             complement(13440..13871)
                     /locus_tag="ACHUOY_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR0682224.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14116..15660
                     /gene="gpmM"
                     /locus_tag="ACHUOY_001406"
                     /gene_synonym="pgmI"
     CDS             14116..15660
                     /gene="gpmM"
                     /locus_tag="ACHUOY_001406"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR0682225.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15694..16953
                     /gene="envC"
                     /locus_tag="ACHUOY_001407"
     CDS             15694..16953
                     /gene="envC"
                     /locus_tag="ACHUOY_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR0682226.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16957..17916
                     /locus_tag="ACHUOY_001408"
     CDS             16957..17916
                     /locus_tag="ACHUOY_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR0682227.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17903..18044)
                     /locus_tag="ACHUOY_001409"
                     /pseudo
     CDS             complement(17903..18044)
                     /locus_tag="ACHUOY_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4545 a         4652 c         4687 g         4160 t
ORIGIN      
        1 tctttttttc ggatttaatt gtgaaatttt aatcatcata ttcatacaat aaattatctg
       61 taaatttttt cccagacatg gtttctttca tatttgacat ttattaataa tcatcattag
      121 aatccacgaa aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt
      181 tttatgatga atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag
      241 caacatagat caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg
      301 aaataaggat ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt
      361 tctctgcgca ttaacgggat gtgactacat tgaagaaggc aagccagaaa gtagtttatt
      421 gaaacaacaa gaagaacatt aaaattgatc tacttgaaaa acaacaagca cagctaaaga
      481 gtcaactgga aactattcaa aaacaacaaa ctggcataat aagcagtaca aagactttaa
      541 ctcacgtaat taaatccgtt aaagatcaac aaaacacttt tatttttacc gagtttaacc
      601 cagcaaaaac gaagtatttc atcctgaaca atggttcggt ggctttagcg ggtcgagtgt
      661 tatccattga cgccacagag aatggcagtg ttattcatat ttcactggtc aacttattaa
      721 gtacacctat ctcaaatatt ggttttaatg cgacatgggg tggtgaaaaa cctgtcgatg
      781 ccaaagagtt tgcccggtgg caacaattac ttttcaacac atcaatgaaa tccacattga
      841 aattattacc aggtcaatgg caagacatta atttgaccct gaagggtgta tcgcccaata
      901 acctgggata tctgaagtta gccatcaaca tggaaaatat tcagttcgac aatcttccct
      961 ctgctgaaaa tcggcagaaa agaagcaaaa aataaatgca gttagtacgc ttataacaag
     1021 gaatagcgag catcattaat gaacaaaata tttaaagtta tctggaaccc tgcgacaggg
     1081 aattatactg ttaccagcga aacggcaaaa agccgtggca agaaatctgg gcgcagtaag
     1141 ctgttaattt ctgcgctggt tgcgggtgga atgttgtcgt cgtttggggc attggcgaat
     1201 gccgggaatg acaacggcca gggtgttgat tacggtagtg gatcagctgg cgacagctgg
     1261 gttgctatag gcaaaggggc gaaagcaaat acttttatga acaccagtgg ttccagtact
     1321 gctgtgggtt atgacgctat agctgaaggc caatatagct ctgccatcgg gtcaaaaacc
     1381 catgcgattg gtggtgcatc aatggccttt ggggttagtg caatatcaga aggcgataga
     1441 agtatagcac tgggtgcctc ttcgtattca ttgggccaat actcaatggc cctcggccgt
     1501 tattcaaaag cattgggtaa attgtctatt gctatggggg actcttccaa agcggaagga
     1561 gcaaacgcca ttgccctggg aaatgccact aaagctactg agattatgag tattgctctt
     1621 ggcgacaccg ccaatgcgtc aaaagcgtat tcaatggcgc tgggagcaag tagcgtcgca
     1681 tctgaagaaa acgcaattgc cctggggcgt agcagtgtag ctagcggtac tgacagcctc
     1741 gcatttggca gacaatcact tgccagcgca gcgaacgcta ttgcgatagg tgctgagacc
     1801 gaagccgctg aaaatgcaac tgctattggc aataatgcga aggcaaaagg gactaatagc
     1861 atggcaatgg ggttcggaag ccttgccgat aaagtcaata ctatcgcatt aggaaatggc
     1921 agccaggctc tggcagataa tgcaatcgcc ataggccagg gcaacaaagc tgatggcgtg
     1981 gatgccatcg ctctgggtaa tggtagccag tcgagaggct taaacaccat tgccttaggc
     2041 acagccagta atgcaactgg tgataagagt cttgcgcttg gtagtaatag cagtgccaac
     2101 ggtattaact ctgtcgcgct gggcgcagat tccattgcgg atttagacaa taccgtctct
     2161 gtcggcaata gttcattaaa acgcaagatc gttaatgtga aaaatggcgc gatcaagtct
     2221 gacagttacg atgccattaa tggttcacag ctttatgcca ttagcgactc ggtagcaaaa
     2281 aggcttggag gaggggctgc agtagatgtt gatgacggta ctgttacagc accaacctac
     2341 aatttaaaaa atggtagcaa aaataacgta ggggctgcgc tcgctgtact tgatgaaaac
     2401 accctgcaat gggaccaaac caaaggcaaa tacagcgctg ctcatggtac tagtagccca
     2461 actgccagcg taatcaccga tgttgcggat ggcacgattt cagcctccag taaggatgcg
     2521 gttaacggtt cccaactgaa agctaccaat gacgatgtcg aagccaacac cgccaatatc
     2581 gctactaata ccagcaacat tgccacgaat acagcaagta ttgccaccaa taccaccaat
     2641 atcaccaacc tgacggattc cgttggtgac cttcaggctg atgccctgct ctggaacgaa
     2701 actaaaaagg cattcagtgc agctcacggc caggatacca ccagcaaaat caccaacgtt
     2761 aaagatgccg acctgacggc tgacagcact gatgctgtta acggctctca gctgaaaacc
     2821 accaacgatg ctgtggcgac gaataccacc aatatcgcca ataacacttc caatattgcc
     2881 actaacacca ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg
     2941 ctgaaatggg ataaggacaa tggtgtattc acggcagctc atggcaccga gaccaccagc
     3001 aaaatcacca acgttaaaga tggcgacctg acgactggca gcaccgatgc cgttaacggc
     3061 tctcagctga aaaccaccaa cgatgccgtg gcgacgaata ccaccaatat cgccactaac
     3121 accaccaaca tctctaatct gactgagacg gtgactaatc ttggtgagga tgcgctgaaa
     3181 tgggataagg acaatggtgt cttcactgca gctcatggca acaataccgc cagcaaaatc
     3241 accaatatcc tggacggcac agtcactgca accagttccg atgccattaa cggtagccag
     3301 ctttatgact taagcagcaa tatcgccacc tacttcggcg gcaatgcttc tgtgaatact
     3361 gacggtgtgt ttaccggtcc aacctacaaa atcggtgaaa caaattatta taacgtcggc
     3421 gatgcactgg ctgcgattaa ctcctcattt agcacgtctc tcggcgatgc tctgctttgg
     3481 gatgccaccg caggtaaatt cagtgccaaa cacggtacta atggtgacgc aagcgtgatc
     3541 actgatgtcg cagatggtga aatttcagac tccagttctg acgcagtaaa cggctcacaa
     3601 ctccacggcg tgagcagtta tgttgttgat gcgctggggg gtggtgccga agtcaatgca
     3661 gacggcacca tcactgcgcc gacgtacacc attgctaatg ctgattacga taatgtcggt
     3721 gatgccctga atgctatcga taccactctt gacgacgctc tgctctggga tgcggacgcc
     3781 ggtgaaaatg gtgcatttag cgccgctcac ggaaaagata aaactgccag tgtaatcact
     3841 aacgtcgcta acggtgcaat ctctgctgcc agcagcgacg cgattaacgg ctcacaactc
     3901 tataccacca ataagtacat cgctgatgcg ctgggtggtg acgcagaagt caacgctgac
     3961 ggcaccatca ccgcaccgac ttacaccatt gcgaacgccg agtacaacaa cgtcggtgac
     4021 gccctggatg cgcttgatga taacgccctg ctgtgggatg agactgccaa tggcggtgct
     4081 ggagcctaca atgccagcca tgacggtaaa gccagcatca tcactaatgt cgctaatggc
     4141 agtattagtg aggacagtac cgatgcagtg aacggttctc agttgaatgc gacgaatatg
     4201 atgattgagc agaacaccca aattatcaat cagctcgctg gtaacaccga cgcaacctat
     4261 atcgaagaaa atggcgctgg tatcaactac gttcgtacta acgacaacga tttagccttt
     4321 aacgatgcaa gcgcctctgg tgtcggcgct acagctgtag gttataacgc tgtcgcgtct
     4381 ggtgccagca gcgtagccat tggtcagaac agcagcagca ccgttgatac cggtattgcg
     4441 ctgggtagca gctccgtttc cagccgtgtg attgccaaag gttctcgtga cactagcgta
     4501 acggaaaatg gcgtggtgat tggttatgac accactgacg gcgaactgct aggtgcattg
     4561 tcaattggtg atgacggtaa ataccgccaa atcatcaacg tagctgatgg ttcagaagcc
     4621 catgacgccg ttacggttcg ccagttgcag aacgctattg gagcggtcgc cactacgcca
     4681 accaagtact ttcacgccaa ctcaacggca gaagactcac tggccgttgg tgaagactcg
     4741 ctggcaatgg gtgcgaaaac tgtcgttaat ggtaatgcag gtattggtat cggtttgaac
     4801 actctggttc tagctgatgc gatcaacggc attgctatcg gcagtaacgc acgcgcaaac
     4861 catgcaaaca gtatcgcaat gggtaacggt tctcagacca cccgtggtgc acagactggc
     4921 tacaccgcct acaacatgga cgcaccgcag aactctgtag gtgagttttc tgtcggtagt
     4981 gaagacggtc aacgtcagat cacaaacgtc gcagctggtt cagcggatac cgatgcggtt
     5041 aacgtgggtc agttgaaagt cactgatgag cgcgtagcgc aaaataccca gagcattact
     5101 aacctgaaca atcaggtcac taatctggat actcgcgtta ctaatatcga aaacggtatt
     5161 ggcgacattg tcaccaccgg tagcaccaag tacttcaaga ccaacaccga tggcgtagat
     5221 gccaacgccc agggtaaaga tagcgttgct attggttctg gttccattgc tgccgctgac
     5281 aacagcgtcg cactgggtac cggttccgtt gcagaggaag aaaatacaat ctctgtaggt
     5341 tcttctacta accaacgccg tatcaccaac gtagctgccg gtgttaatgc caccgatgcg
     5401 gttaacgtgt cgcagctgaa atcttctgaa gcaggcggcg tgcgctacga caccaaagct
     5461 gatggttcta tcgactatag caatatcacc ctcggtggtg gcaacggcag tacgactcgt
     5521 atcagcaacg tctccgctgg cgtcaacaac aacgacgcgg tgaactacgc gcagttgaag
     5581 caaagcgtgc aggaaacgaa gcaatacacc gatcagcgga tggttgagat ggataacaaa
     5641 ctgtctaaaa ctgaaagcaa gttgagcggt ggtatcgctt ctgcaatggc aatgaccggt
     5701 ctgccgcagg cttatacacc gggtgccagc atggcttcta ttggtggcgg tacttacaac
     5761 ggtgaatcgg cagttgcttt aggtgtatcg atggtgagcg ccaatggtcg ttggatctac
     5821 aaattacaag gtagtaccaa tagccagggt gaatactccg ccgcactcgg tgccggtatt
     5881 cagtggtaat gatccattaa gttagtgtga ctaagggcaa acgccaaatt gcctgatgcg
     5941 ttacgcgcat caggcaagat aaggcgcccc gggtcaacaa tctaagggca attctctgat
     6001 gaggattgcc cttttcttta ccagacatct ccccccacaa gaattggccc taccaattct
     6061 tcgcttatct gacctctggt tcacaattcc ccaattaaaa ctcacatcaa tattgccaat
     6121 acataacatt tagttaacca ttcattgtca ttatccctac acaacacaat tggcagtgcc
     6181 acttttacac aacgtgtgac aaggagatga gcaacagact cattacacga tgtgcgtgga
     6241 ctccaggaga cctgcaatga atctctggca acaaaactac gatcccgccg ggaatatctg
     6301 gctttccagt ctgatagcat cgcttcccat cctgtttttc ttctttgcgc tgattaagct
     6361 caaactgaaa ggatacgtcg ccgcctcatg gacggtagca atcgcccttg ccgtggcttt
     6421 gctgttctat aaaatgccgg tcgctaacgc gctggcctcg gtggtttatg gcttcttcta
     6481 tggtttgtgg cccatcgcgt ggatcattat tgcagcagtg ttcgtctata agatctcggt
     6541 gaaaaccggg cagtttgaca tcattcgctc gtctattctt tcgataaccc ctgaccagcg
     6601 tctgcaaatg ctgatcgtcg gtttctgttt cggcgctttc cttgaaggag ccgcaggctt
     6661 tggcgcaccg gtagcaatta ccgccgcatt gctggtcggc ctgggtttta aaccgctgta
     6721 cgccgccgga ctgtgcctga ttgttaacac cgcgccagtg gcatttggcg cgatgggcat
     6781 tccaatcctg gttgccggac aagtaacagg catcgacagc tttgagattg gccagatggt
     6841 ggggcggcag ctaccgttta tgaccattat cgtgctgttc tggatcatgg cgattatgga
     6901 cggctggcgc ggtatcaagg agacgtggcc tgcggtcgtg gttgcgggcg gctcgtttgc
     6961 catcgctcag taccttagct ctaacttcat tgggccggag ctgccggaca ttatctcttc
     7021 gctggtatca ctgctctgcc tgacgctgtt cctcaaacgc tggcagccag tgcgcgtatt
     7081 ccgttttggt gatttggggg cgtcaaagat tgatatgacg ctggcccaca ccggttacac
     7141 tgcgggtcag gtgttacgtg cctggacacc gttcctgttc ctgacagcta ccgtaacact
     7201 gtggagcatc ccgccgttta aagccctgtt cgcatcgggt ggtgcgctgt atgagtgggt
     7261 gatcaacatt ccggtgccgt acctcgataa actggttgcc cgtatgccac cagtggtcag
     7321 cgaggctaca gcctatgccg ccgtgtttaa gtttgactgg ttctctgcca ccggcaccgc
     7381 cattctgttt gctgcactgc tctcgattgt ctggctgaag atgaaaccgt ctgacgctat
     7441 cagcaccttc ggcagcacgc tgaaagaact ggctctaccc atctactcca tcggcatggt
     7501 gctggcgttc gcctttatct cgaactattc cggactgtca tcaacactgg cactggcact
     7561 ggcgcacacc ggtcatgcat tcaccttctt ctcgccgttc ctcggctggc tgggggtatt
     7621 cctgaccggg tcggatacct catctaacgc cctgttcgcc gcgctgcaag ccaccgcagc
     7681 acaacaaatt ggcgtctctg atctgttgct ggttgccgcc aataccaccg gtggcgtcac
     7741 cggtaagatg atctccccgc aatctatcgc tatcgcctgt gcggcggtag gcctggtggg
     7801 caaagagtct gatttgttcc gctttactgt caaacacagc ctgatcttca cctgtatggt
     7861 gggcgtgatc accacgcttc aggcttatgt cttaacgtgg atgattcctt aatgattgtt
     7921 ttacccagac gcctgtcaga cgaggttgcc gatcgtgtgc gggcgctgat tgatgaaaaa
     7981 aacctggaag cgggcatgaa gttgcccgct gagcgccaac tggcgatgca actcggcgta
     8041 tcacgtaatt cactgcgcga ggcgctggca aaactggtga gcgaaggcgt gctgctcagt
     8101 cgacgcggcg gcgggacgtt tattcgctgg cgtcatgaca catggtcgga gcaaaacatc
     8161 gtccagccgc taaaaacgct gatggccgat gatccggatt acagtttcga tattctggaa
     8221 gcccgctacg ccattgaagc cagcaccgca tggcatgcgg caatgcgcgc cacacctggc
     8281 gacaaagaaa agattcagct ttgctttgaa gcaacgctaa gtgaagaccc ggatatcgcc
     8341 tcacaagcgg acgttcgttt tcatctggcg attgccgaag cctcacataa catcgtgctg
     8401 ctgcaaacca tgcgcggttt cttcgatgtc ctgcaatcct cagtgaagca tagccgtcag
     8461 cggatgtatc tggtgccacc ggttttttca caactgaccg aacaacatca ggctgtcatt
     8521 gacgccattt ttgctggtga tgccgacggg gcgcgtaaag caatgatggc gcaccttagt
     8581 tttgttcaca ccaccatgaa acgattcgat gaagatcagg ctcgccacgc acggattacc
     8641 cgcctgcccg gtgagcataa tgagcattcg agggagaaaa acgcatgatt atttccgcag
     8701 ccagcgatta tcgcgccgca gcgcaacgca ttctgccgcc gttcctgttc cactatatgg
     8761 atggtggtgc atattctgaa tacacgctgc gccgcaacgt ggaagatttg tcagaagtgg
     8821 cactgcgcca gcgtattctg aaaaacatgt ccgacttaag cctggaaacg acgctgttta
     8881 atgagaaatt gtcgatgccg gtagcactgg caccggtggg tttgtgtggc atgtatgcgc
     8941 gtcgtggcga agttcaggca gccaaagcgg cggacgcgca tggcattccg tttactctct
     9001 cgacggtttc cgtttgcccg attgaagaag tcgcgccagc catcaagcgc ccaatgtggt
     9061 tccagcttta tgtactgcgc gatcgcggct ttatgcgtaa cgcgctggag cgagcaaaag
     9121 cagcgggttg ttcgacgctg gttttcaccg tggatatgcc gacaccgggc gcacgctacc
     9181 gtgatgcgca ttcaggtatg agcggcccaa atgcggcaat gcgccgctac ttgcaggcgg
     9241 tgacgcatcc gcaatgggcg tgggatgtgg gcctgaacgg tcgtccacat gatttaggta
     9301 atatctcagc ttatctcggc aaaccgaccg gactggaaga ttacatcggc tggctgggga
     9361 ataacttcga tccgtccatc tcatggaaag accttgagtg gatccgcgat ttctgggatg
     9421 gcccgatggt gatcaaaggg atcctcgatc cggaagatgc gcgcgatgca gtacgttttg
     9481 gtgctgatgg aattgtggtt tctaaccacg gtggccgcca gctggacggt gtactctctt
     9541 ccgctcgtgc gttgcccgct attgcagatg cggtgaaagg tgatatcgcc attctggcgg
     9601 atagcggaat tcgtaacggg cttgatgtcg tgcgtatgat tgcgctcggt gccgacaccg
     9661 tactgctggg tcgtgctttc ctgtatgcgc tggcaacagc gggccaggcg ggtgtagcta
     9721 acctgctaaa tctgatcgaa aaagagatga aagtggcgat gacgctgact ggcgcgaaat
     9781 cgatcagtga aattacgcaa gattcgctgg tgcaggggct gggtaaagag ttgcctacgg
     9841 cactggctcc gatggcgaaa gggaatgcgg catagtcgtt tgcgccccct caccctaacc
     9901 ctctccctca gggagagggg accgttcggc gctgtatgta ctccctcact ctgaaacgac
     9961 accgcactct ttttttctcc ctcgcccctc cggggagggc cggggtgagg ggaaaaggcc
    10021 gcacggttca ccaattttct gctatcctgc ccccgcacta agggggcagt atgctaaaca
    10081 tcgtacttta cgaaccagaa attccgccaa atactggcaa catcatccgt ctttgcgcta
    10141 ataccggctt tcgtctgcat atcatcgaac cgatgggatt tgcctgggac gataagcgcc
    10201 tgcgccgcgc ggggctggac tatcacgagt ttaccgccgt tacgcgtcat catgactatc
    10261 gcgcgttcct cgaagcagaa aatccccagc gcctgtttgc cctcaccacg aaaggtacgc
    10321 ctgctcacag cgccgtaagc tatcaggatg gcgattatct gatgttcggc ccggaaacac
    10381 gcggcctgcc agcgagcatt cttgatgccc tgcccgctga acaaaaaatt cgcattccga
    10441 tggtgccgga cagccgcagc atgaatctgt ccaatgcggt gtcggtagtg gtgtatgaag
    10501 cctggcggca gctgggatat ccgggagcgt tgttgagaga ttagttactg tatgccggat
    10561 agatgattac atcgcatccg gcacgatcac aggacattag atcccatccc catactcaaa
    10621 tgtatggtta ataccgttga aatgctggtc catatccatt gatggcttat cgctgtctgg
    10681 tttaccgaca atacgagccg gaacgccagc ggcggttgta tgcggcggca ccggttgcag
    10741 caccacggaa cctgcgccaa tcttcgcgcc gcgaccaact tcaatattgc cgaggatttt
    10801 cgcgcccgcg ccaatcatca caccttcacg aattttcggg tgacggtcac caccagattt
    10861 acccgtaccg ccaagcgtca cagattgcag aatcgatacg tcgttttcaa ttaccgccgt
    10921 ttcacccaca acgatgcccg tggcgtggtc gagcataatg ccacggccaa tttttgctgc
    10981 cgggtgaata tcgacctgga acgtcacaga aacctggttt tgcagaaaga ttgccaatgc
    11041 gcgacgcccc tgattccaca accagtgacc gatgcgatag gcctgcaagg catgaaaacc
    11101 cttcaggtat aacaacgggg ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc
    11161 ctgaatatca caggccgcag aggcgatcat ttccgggtca gcggcgtagg cttcttccac
    11221 cacttcacgg atagcaatag caggcataat tggcgatgac agcttgttcg ccagcatgta
    11281 gctcagtgca ctaccaaggt tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag
    11341 cattggctca cagtccgcca gcgttctggc ttcggcttta atattgttcc agacaatttc
    11401 cagttcttca cacgacattg cttactccac acgatgagat aatgaccggc ccgttctgcg
    11461 cgggccgggt catagcggta acaaaggttc cctggggtta gtggctgctg cgctcgtcct
    11521 tgcgtgcacg acctaataac gtcaatgctg cctcgcgcgc gttttttccg caatataata
    11581 cttgataaat ttcctcggtt attggcattt ctacgccgaa gcgatgcgcc agttcgcgga
    11641 cttctttcgt attgcggtag ccttccacca cctgaccaat cttctcctgc gcgctttgta
    11701 catccatgcc ctgaccgagc atcatgccaa aacggcggtt acgcgactgg ttgtcggtac
    11761 aggtaagcac cagatcgccc agccccgcca tgcccataaa ggtggcagga tcggcaccca
    11821 gcgccgcgcc aagacgcgac atttcagcca gcccacgggt gatcagtgcc gtacgcgcat
    11881 tcgcaccaaa accgataccg tcggacatcc ccgcgccaat ggcaatgacg tttttcaccg
    11941 caccgccaag ctgcacaccg atgaaatccg ggttgctgta aacgcggaaa cttttgccac
    12001 agtgcagcaa ttgttggaga tcatcggcaa aggtctggtc ggtagaggcc aacgaaatag
    12061 ccgtcggtaa acctgccgcc agttctttcg caaacgttgg gccagagata accgccagcg
    12121 gaatttgatc gcctaaggcc tcacgcgcca cgtcctgtaa cagacgtccg gtttccgctt
    12181 ccagcccttt ggtcgcccac accagacgcg catcaggacg catcagcggt ttaatctggc
    12241 gcagcacttc accaaagaca tggctgggta cgacgacgag aatattacgg ctggctgcca
    12301 gcgcagtggc gagatcgctt tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa
    12361 acgcggcgtt acagcggtcg cgttcaagcg ttgcgatatg ttcagggtca tggccccaga
    12421 ggacaacctc gtggccattt cttgccaggg taatggcaag agcggtgccg tacgagccgg
    12481 caccgatcac agtcattgaa gcattacgtt ggttcatcag gcatcctgat gttcttcagt
    12541 accttcgcca gcctgctgct gcaaatagtt catgaacagc gcatcgaagt taatcggcgc
    12601 aaggttcagt tgcgggaatg taccgcggga taccacgctg gtgatgcact cacgagcata
    12661 cgggaacaga atgttcgggc agtatgcgcc caggcaatgt gccatctggg tgccttcgat
    12721 acccgcgatg gagaaaatac cgccctgctg aacttcacac aggaacgcgg tttcttcgcc
    12781 cagggaggcc gttacggtaa cacgcagtac cacttcgtat acgtcatctg ccagttggga
    12841 agatgccgta tccagatcaa gtttaacttc tggttgccaa tctttctgga aaacgtgcgg
    12901 cgcgttcggc gcttcgaaag agatatcctt ggtataaata cgttggatct ggaaagtcat
    12961 ttcagtgttg ttttgttctg acatgtgtag aaaaccctta agtgttgtcc ttaaatactg
    13021 cgcaatgtaa tgccaacgtt cacacgttat ttcagcaggg gatccagtcc accacgtgca
    13081 tccaatgcat acaagtcatc acagccgcca atgtgctgtg cgtcaataaa aatctgggga
    13141 accgtggtgc gaccgctgcg tttgatcatc tcttcacgct tggcggcatt gccatcgatc
    13201 ggcaactcct gaaaactcac gcccttgctg ctcagcagtg cttttgcacg atggcaatac
    13261 gggcaggttt ctttggtata gatttcaaca ttggccataa tttagctcct gtttttttac
    13321 cctgaaattt catgttgcag ggcggctgca aaatggagtg tccccagaag cttataacaa
    13381 taagtcgctg ggggcctaat tgcagctaac ggcctgcatc atgaaagacg acaggtaaat
    13441 tatttgccgc gcaccagagg caagttttcg ccagcccagc cagcaacgcc ctctttcagc
    13501 acgaatactt gcgcaaaacc agctttcgtc agcgcgtttg caggctcctg gcactgcatg
    13561 ccagaaccgt ctaccacgat aaccggtttg tctttgtgct tctcaagctc accaacattg
    13621 ttggctttga tttcgctcgg caacagattg atagaacctg cgatatggcc tttacggaag
    13681 tcatcacgct gacgtaaatc cacaaccaca gcgtcttctt tgttgatcag acgcgtagct
    13741 tcaccacgag taatcacctt cactttcgag gtcaggctct taaacgtagt cacaagaacc
    13801 gccaccagta acgcgatcca ggcgatactc agtatgggat gacggccaac aaattgcata
    13861 atttcttgca tggggggtaa caactcccga cgtagtgatt aaaaaaacca ggaaaggagt
    13921 atacctgcgc ggtgcggcaa atacagccag cgcgttaact ggaatgcaat tttgcggggc
    13981 acgacgaaaa aaaagcgcaa atctggcatc ggcttgccgc gagcggtcgt atattttgat
    14041 cttcagaggc tattttatcg attcagctgt agtaaaatta cgcaaatttt gactcttgag
    14101 tatgaggttg tcgcaatgtc ggtttctaaa aaacctatgg tactggtgat tctggatgga
    14161 tatggctatc gtgaagaaca gcaggataac gccattttta gtgctaaaac cccggtaatg
    14221 gatgcactgt gggccaatcg tccgcatacc ctgatcgacg cttccggtct ggaagtcggt
    14281 ctgcctgacc gtcagatggg taactccgaa gtaggccacg ttaacctggg tgccggccgc
    14341 atcgtgtatc aggacctgac tcgtctggac gttgaaatca aagatcgcgc tttctttgct
    14401 aatccagtgc tgactggcgc ggtagataaa gcgaaaaacg caggtaaagc agtacacatt
    14461 atgggtctgc tctccgcagg cggcgtacac agccacgaag atcacatcat ggcgatggta
    14521 gaactggcag ctgaacgcgg cgcagaaaaa atctacctgc acgcattcct tgacggtcgc
    14581 gacaccccgc cgcgcagtgc tgaatcctcg ctgaaaaaat ttgaagagaa atttgctgcg
    14641 ctgggtaaag gccgcgtagc gtccatcatt ggtcgttact acgcgatgga ccgcgataac
    14701 cgttgggatc gcgttgaaaa agcctatgac ctgctgactc tggcgcaggg tgagtttcag
    14761 gccgatactg ccgttgctgg tttgcaggca gcttatgctc gcgacgaaaa cgacgagttc
    14821 gtgaaagcga ccgttattcg tgcagaaggt cagccagatg cggcaatgga agacggcgat
    14881 gcgctgattt tcatgaactt ccgtgctgac cgcgcgcgtg aaatcactcg tgcattcgtg
    14941 aacgctgatt tcgatggctt cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg
    15001 accgaatacg ccgctgacat caaaactgcg gttgcttacc cacccgcttc cctggttaac
    15061 accttcggcg agtggatggc gaaaaacgac aaaactcagt tgcgtatttc cgaaaccgaa
    15121 aaatatgccc acgttacttt cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa
    15181 gatcgcattc tgatcaactc accgaaagtg gctacctacg atctgcaacc ggaaatgagc
    15241 tccgcagagc tgaccgaaaa actggttgcg gccatcaaga gcggcaaata cgacaccatt
    15301 atctgtaact atccgaacgg cgacatggta ggtcacaccg gggtgatgga agcggcggtt
    15361 aaagcggttg aagcactgga tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg
    15421 ggtggacaac tgctgatcac cgctgaccac ggtaacgctg aacagatgcg cgatccggca
    15481 acgggtcagg cacacacggc acacaccaac ctgccagttc cgctgattta cgttggtgat
    15541 aagaacgtga aagcggttgc aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg
    15601 ctgatgggta tggaaatccc gcaagagatg actggtaagc cgctgttcat cgtggaataa
    15661 tccctcccca tgaggggaaa ggcgattaat accatgacac gggccgtgaa accgcgcagg
    15721 tttgcaatca ggcccatcat ctacgccagc gttcttagcg ctggcgtatt gttgtgcgcc
    15781 ttttccgccc acgcggatga gcgtgaccaa ctcaaatcta ttcaggccga tatcgccaca
    15841 aaagagcgcg cggtacgcca aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa
    15901 aagcaggaag aagcgatctc tgaagccacc cgtaagctgc gcgaaacgca aaacacgctc
    15961 aatcaactca ataaacagat tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa
    16021 aaagccgccc aggagcgcag cctcgccgca caactggatg ccgcattccg tcagggcgag
    16081 cataccggta ttcagctaat tctcagcggt gaagaaagcc agcgtggaca gcgtttacag
    16141 gcttatttcg gctatctcaa ccaggcgcga caagaaacca ttgcccagtt gaagcaaacg
    16201 cgtgaagaag tcgccatgca gcgtgctgaa ctggaagaga aacaaagcga gcaacaaacg
    16261 ctgttatatg aacagcgcgc ccaacaggcg aaactgactc aggctctgaa cgagcgtaaa
    16321 aagacgctgg cggggctgga atcttccatc cagcaaggtc agcagcagct aagtgaactg
    16381 cgtgccaacg aatcccgtct gcgtaacagt attgcccgtg cggaagccgc ggcgaaagcg
    16441 cgtgctgaac gagaagcgcg tgaggctcag gcagttcgcg acaggcagaa agaagcgacg
    16501 cgcaaaggca ccacctacaa accgaccgaa agcgaaaaat cgctgatgtc ccgaactggt
    16561 ggcctgggtg cgccgcgtgg tcaggcattc tggccagttc gtggccctac gctgcatcgc
    16621 tatggcgagc agctacaggg tgaattacgc tggaaaggaa tggttatcgg tgcctctgaa
    16681 ggtactgaag ttaaagcaat tgccgacggt cgggtgattc tggctgactg gctgcaaggc
    16741 tacggtctgg tggtggtggt tgagcacggt aaaggcgata tgagtcttta cggctataat
    16801 caaagcgcgc tggtgagcgt tggttcgcag gttcgtgcgg gccagccaat tgcactggtg
    16861 ggcagcagtg gcggtcaggg cagaccttca ctctatttcg aaattcgccg ccagggtcag
    16921 gccgtcaatc cacagccgtg gttgggaaga taagttttgt ttccatttcg tcgtaacgtt
    16981 cttgcatttg ccgctctgtt ggcgctctcc tcccctgtaa ttgctggcaa acttgccatc
    17041 gtcattgatg attttgggta tcgcccgaac aacgaaaacc aggtgctggc gatgccttcc
    17101 gctatctccg tcgctgtatt acccgattca ccgcacgcca gagagatggc gaccaaagcg
    17161 cataacagcg gtcacgaagt gttgattcat ctcccgatgg caccgttgag taaacagccg
    17221 ctggagaaaa atacgctgcg cccggagatg agcagcgacg aaattgagcg cattattcgt
    17281 agtgcggtca ataacgtgcc ctatgccgtg gggatcaata accacatggg cagcaagatg
    17341 acctctaacc tgtttggtat gcagaaagtg atgcaggcgc tggagcgtta taatctttac
    17401 ttcctcgaca gcgtaaccat tggtaatacc caggcgatgc gcgccgcgca aggcactggc
    17461 gtgaaggtga tcaaacggaa ggtgttcctc gacgattcac agaacgaagc ggacatccgt
    17521 gtgcaattca atcgcgcaat tgacctagcg cgtcgcaacg gatcaaccat tgccattggg
    17581 catcctcacc cttcaacggt acgcgtgttg caacagatgg tttataacct gccgccagac
    17641 attacgctgg tgaaagccag cagcttgctg aatgaaccgc aggttgatac gtctacaccg
    17701 ccgaaaaaca ctgtgcccga cacaccgcgt aatccgttcc gtggcgtgaa gctgtgcaaa
    17761 ccgaagaaac cgatagaacc tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc
    17821 atcagccaga gcacgctgat cgtttacttc cagcatcagt ggcaaggctg gggcaaacag
    17881 cctgaagcgg cgaagcttaa cgctagcgta aattaaaggc gctacgcgta atgcgatggt
    17941 gcgaccgcgt tttgtcgcgc cattgccata agcggaaaga ctacagtaac gcctgataac
    18001 ccacctttac gctgcgcaca ttggtaatca ggcgcttata cata
//
LOCUS       DAZOYL010000057        18003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZOYL010000057 DAZOYL010000000
VERSION     DAZOYL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.057"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            129..944
                     /gene="mepH"
                     /locus_tag="ACHUOY_001410"
     CDS             129..944
                     /gene="mepH"
                     /locus_tag="ACHUOY_001410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR0682228.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1072..1653
                     /gene="sodB"
                     /locus_tag="ACHUOY_001411"
     CDS             1072..1653
                     /gene="sodB"
                     /locus_tag="ACHUOY_001411"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR0682229.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1799..2974)
                     /locus_tag="ACHUOY_001412"
     CDS             complement(1799..2974)
                     /locus_tag="ACHUOY_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0682230.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLL
                     LLMVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3140..3229)
                     /gene="cydH"
                     /locus_tag="ACHUOY_001413"
                     /gene_synonym="ynhF"
     CDS             complement(3140..3229)
                     /gene="cydH"
                     /locus_tag="ACHUOY_001413"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR0682231.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3528..4553
                     /gene="purR"
                     /locus_tag="ACHUOY_001414"
     CDS             3528..4553
                     /gene="purR"
                     /locus_tag="ACHUOY_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR0682232.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4550..5482)
                     /gene="punR"
                     /locus_tag="ACHUOY_001415"
     CDS             complement(4550..5482)
                     /gene="punR"
                     /locus_tag="ACHUOY_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR0682233.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5595..6806
                     /gene="punC"
                     /locus_tag="ACHUOY_001416"
     CDS             5595..6806
                     /gene="punC"
                     /locus_tag="ACHUOY_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR0682234.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7097..8245
                     /gene="cfa"
                     /locus_tag="ACHUOY_001417"
     CDS             7097..8245
                     /gene="cfa"
                     /locus_tag="ACHUOY_001417"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR0682235.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8285..8926)
                     /gene="ribE"
                     /locus_tag="ACHUOY_001418"
     CDS             complement(8285..8926)
                     /gene="ribE"
                     /locus_tag="ACHUOY_001418"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR0682236.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9141..10514
                     /gene="mdtK"
                     /locus_tag="ACHUOY_001419"
     CDS             9141..10514
                     /gene="mdtK"
                     /locus_tag="ACHUOY_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR0682237.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(10555..11811)
                     /locus_tag="ACHUOY_001420"
     CDS             complement(10555..11811)
                     /locus_tag="ACHUOY_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0682238.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12119..12195
                     /locus_tag="ACHUOY_001421"
     tRNA            12119..12195
                     /locus_tag="ACHUOY_001421"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12153..12155,aa:Val,seq:gac)
     gene            12200..12276
                     /locus_tag="ACHUOY_001422"
     tRNA            12200..12276
                     /locus_tag="ACHUOY_001422"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12234..12236,aa:Val,seq:gac)
     gene            12384..12689
                     /locus_tag="ACHUOY_001423"
     CDS             12384..12689
                     /locus_tag="ACHUOY_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR0682239.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12815..14419
                     /locus_tag="ACHUOY_001424"
     CDS             12815..14419
                     /locus_tag="ACHUOY_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR0682240.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(14431..15243)
                     /gene="ydhT"
                     /locus_tag="ACHUOY_001425"
     CDS             complement(14431..15243)
                     /gene="ydhT"
                     /locus_tag="ACHUOY_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR0682241.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15247..16032)
                     /gene="phsC"
                     /locus_tag="ACHUOY_001426"
     CDS             complement(15247..16032)
                     /gene="phsC"
                     /locus_tag="ACHUOY_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR0682242.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     VVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16029..16697)
                     /locus_tag="ACHUOY_001427"
     CDS             complement(16029..16697)
                     /locus_tag="ACHUOY_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0682243.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(16761..17399)
                     /locus_tag="ACHUOY_001428"
     CDS             complement(16761..17399)
                     /locus_tag="ACHUOY_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR0682244.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(17412..>18003)
                     /locus_tag="ACHUOY_001429"
     CDS             complement(17412..>18003)
                     /locus_tag="ACHUOY_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HFR0682245.1"
                     /translation="GLPLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLR
                     VCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIF
                     TLHRAYTVKLMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYK
                     EMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         4471 a         4696 c         4255 g         4581 t
ORIGIN      
        1 gctcgccaac gaaacgtatt ttttaacaat aaaagctatt aactttctct tcttctatgc
       61 attagaatca tcaagttttg taaatcagac gcaggcatga tagacctgcc ttaacagagg
      121 gacgctcagt ggcgcggata aaccgtattt cgatcacgct ctgtgcttta ctttttacca
      181 ccctgccttt aacgcctatg gcccatgctt caaagcaagc cagggagagt tctgctacca
      241 ctcatatcac caaaaaagca gataaaaaga aaagcacggc aaccaccaaa aagacccaga
      301 aaacagcgaa aaaagccgcc agtaaaagta cgaccaaaag caaaaccgct tcttccgtta
      361 aaaaatcttc cattaccgct tctaaaaacg ccaaaactcg cagcaaacac gccgtcaata
      421 aaacggcctc agccagcttt accgaaaagt gtaccaaacg taagggctat aaatcgcatt
      481 gtgtgaaagt caaaaatgcc gcgtcaggaa ctcttgccga cgcgcacaaa gcgaaggtgc
      541 aaaaagctac gaaagtggca atgaataaac tgatgcagca aattggtaag ccatatcgtt
      601 ggggtggcag ctcaccgcgt accggttttg attgcagcgg cctggtttat tacgcttata
      661 aagatttggt gaaaattcgt attccgcgta cggcgaatga aatgtatcac ctgcgtgatg
      721 cagcgccaat cgaacgtagt gaactgaaaa acggcgacct ggtctttttc cgtactcagg
      781 gacgcggcac agccgatcat gtcggcgtgt atgtcggcaa cggcaaattt attcagtcac
      841 cgcgcacagg tcaggaaatt caaatcactt ctctcagtga agactactgg cagcgccact
      901 atgttggcgc tcgtcgggta atgaccccaa aaacacttcg ctaaaacttt accctgttgt
      961 tacggcaaca gggtaagttc atcttttgtc tcacctttta atttgctacc ctatccatac
     1021 gcacaataag gctattgtac gtatgcaaat taataataaa ggagagtagc aatgtcattc
     1081 gaattacctg cactaccata tgctaaagat gctctggcac cgcacatttc tgcggaaact
     1141 atcgagtatc actacggcaa gcatcatcag acttatgtca ctaacctgaa caacctgatt
     1201 aaaggtaccg cgtttgaagg taaatcactg gaagagatta ttcgcagctc tgaaggtggc
     1261 gtattcaaca acgcagctca ggtctggaac catactttct actggaactg cctggcaccg
     1321 aacgccggtg gcgaaccgac tggaaaagtc gctgaagcta tcgccgcatc ttttggcagc
     1381 tttgccgatt tcaaagcgca gtttactgat gcagcgatca aaaactttgg ttctggctgg
     1441 acctggctgg tgaaaaacag cgatggcaaa ctggctatcg tttcaacctc taacgcgggt
     1501 actccgctga ccaccgatgc gactccgctg ctgaccgttg atgtctggga acacgcttat
     1561 tacatcgact atcgcaatgc acgtcctggc tatctggagc acttctgggc gctggtgaac
     1621 tgggaattcg tagcgaaaaa tctcgctgca taataactga tggcaaatgc agcattgcct
     1681 gatgcgcttc gcttaccatg ccaacgcgga tgaggcgtta acagcgcatt cggcaagata
     1741 aatcgcacgt tgtcagcaac tgtaacgcag aaggttatcc ttctgcgttt ttgtttaatt
     1801 agctgttggc aacgcaaact gtttcaggtt gttttctggc tgacataaac accagcaata
     1861 atgccagtcc cgcgacaatc gcccccatca ccggcacaaa gctgtatccc agcccagcgg
     1921 aaattaccgc accaccagcc gctgctccca gcgcatttcc aagattaaag gcaccaatat
     1981 tgactgatga agacagacct ggcgcttcac tggcgacacg catcacgcgc atctgtaacg
     2041 gcggtacgac cgcaaaggtt gctgcgcccc acaccaccat gctaatagct gcgccgaact
     2101 cattgcgggc caggaacggg gttgccagca taatcaccat cagcagcaac aaaaagcctt
     2161 tcaacgtgcc gttaactgaa cgatctgcca gtttgccgcc gagatagtta ccgatagaga
     2221 atccgacacc aatcagcacc agcattgccg tgacgaacac cggtgttgcg tgggtaatac
     2281 tttgcagtac cggagagata taggtgtaga gagtaaacat tgcgccagct cccagtaccg
     2341 tcgtcagcaa tgcagacagc acctgcggac gcattaatac cgccagctct tttttcactt
     2401 caggtcgtgc ccctgtacca cctttaggta atgagaagaa cagacttacc attgaaatca
     2461 ctcccagccc cgccgttgcc agaaatgaca tccgccagcc gatggtttca cccaaccagg
     2521 tcgccgccgg cacgccaccg atatttgcca gggttaaccc cataaacata gtggcaactg
     2581 cgctggcctg tttatgtttt ggcaccacgc ttgcggccac gactgaaccc aaaccaaaaa
     2641 atgctccgtg attcaggctg gtcaaaatgc gtgaaagcat cagggtcata taatccggcg
     2701 cgatggcgga aagtacgttg ccgagcgtga aaattgccat caggaaaatc aacgcactgc
     2761 ggcgggcacg atgagaaagt agaagcgtca tcagcggcgc gcccaccatt acgccaactg
     2821 cataggcact gattaacatt ccggcagcgg gaatcgagac atccacaccg cgcgcaatga
     2881 cgggcaacaa gcccattggc gagaactccg ttgtcccgat accaaacgcg ccaatcgcca
     2941 gcgccagcgc cagcaacgga tagttaattt tcatgcctta tctccacctc ttcgcgtcat
     3001 tacgcgatat tcattaaagt ggcgaaagca tgacagcaat cacaaaaaaa tgaaaataac
     3061 aaaaagagaa aacacttttg ccattttgct aacaaacagg aaggagatgc gagggagaac
     3121 gcgctccctc gagaggaaat cagtgcagcg cggcagtcaa acccacggct acgatcaaac
     3181 cgaggacgat aatcgttgtt accagtgaaa atttaaggtc ggtgctcatc aagttttctc
     3241 cttttttatt accacacaaa aagtgatatt acgcattttt acacactgtg atgaaaaaat
     3301 ctcccgtcat ttataatgat aagtgttttt accacttccc cttttcgtca agatcggcca
     3361 aaattccacg cttacactat ttgcgtactg gccattgacc ccttcctgac gctccgtgtc
     3421 gtttttccgg cgtaccgcaa cacttttgtt gtgcgtaagg tgtgtaaagg caaacgttta
     3481 ccttgcgatt ttgcaggagc tgaagttagg gtctggagtg aaatggaatg gcaacaataa
     3541 aagatgtagc gaaacgagca aacgtttcca ctacaactgt gtcacacgtg atcaacaaaa
     3601 cacgtttcgt cgctgaagaa acgcgcaacg ccgtgtgggc agcgattaaa gaattacact
     3661 actcccctag cgcggtggcg cgtagcctga aggttaacca caccaagtct atcggtttgc
     3721 tggcgaccag cagcgaagcg gcctattttg ccgagatcat tgaagcagtt gaaaaaaatt
     3781 gcttccagaa aggttatacc ctgattctgg gcaatgcgtg gaacaatctt gagaaacagc
     3841 gggcttatct gtcgatgatg gcgcaaaaac gcgtcgatgg tctgctggtg atgtgttctg
     3901 agtacccaga gccgttgctg gcgatgctgg aagagtatcg ccatatccca atggtggtga
     3961 tggactgggg tgaagcaaaa gctgacttca ccgatgcggt cattgataac gcgttcgaag
     4021 gcggctacat ggccgggcgt tatctgattg aacgcggtca ccgcgaaatc ggcgttatcc
     4081 ccggcccgct ggaacgtaac actggcgcag gccgccttgt cggttttatg aaggcgatgg
     4141 aagaagcaat gatcaaggtg ccggaaagct ggattgtgca gggtgacttt gaacctgaat
     4201 ccggttatcg cgccatgcag caaatcctgt cgcagccgca tcgccctact gccgtcttct
     4261 gtggtggcga tatcatggca atgggcgcac tttgtgctgc tgatgaaatg gggctgcgcg
     4321 tcccgcagga tgtttcgcta attggttatg ataacgtgcg caacgcccgc tattttacgc
     4381 cggcgctgac cacaatccat cagccaaaag attcgctggg tgaaacagcg ttcaacatgc
     4441 tgttggatcg tatcgtcaac aaacgtgaag aaccgcagtc cattgaagtg catccgcgct
     4501 tgattgaacg ccgctccgtg gctgacggcc cgttccgcga ctatcgtcgt taatcacccg
     4561 ttgcgggagt ctcttccggc tcccgcagcc actccttatt cagcgtctca ctatcgccga
     4621 gatactcaag caaccaggtt aacgcaggcg acatatcatt ttgctgccat gtcagacaac
     4681 atgccgaatc cggaaagggg ttttccagtt caagtgctac ccacttcccc tcattaagcc
     4741 acggtttggc gaaatgtgtt ggcaccatcc ctatgcataa tcctgccgag atacaggttg
     4801 ccgatgattc ccagtcaggc acgacaactc ttttttggtt atccagcaac caggtaatac
     4861 gtttaggtag cgttcgcgag gtgtcttcgc gcaccaacga cggccagttg cgcaacgtat
     4921 catcgctgaa cgggccatcc atcaacgcca gcgggtgatg gctggcaaca acgcaactcc
     4981 agcttagcat ccccatatcc cggaaggcat aacgaccgcc taccggaatc gcgcgtgttg
     5041 cgccaatcgc cagttccacg cgcccgtcgg aaagcgcatc ccagacaccg ttgaacactt
     5101 cctgaaagac aagaagttcg acatcatcaa aatggcgata aaaatcaacg atcatctgcc
     5161 gtgtacgttc tggcctgaca atattatcca ctgcgatagc taactgaccg cgccagccgt
     5221 tcgctatctg ctgacattgc tggcgggtga tctgcatttt tttgacaaca gagcgccctt
     5281 ctttgagaaa ccacgctcca gcagcggtca gctccacatc acggtgccgt cgttcaaaga
     5341 gcggcaccgc cagccactct tccagctgac gcacggtata gctgaccgca gaaggaacgc
     5401 gatgcagctc ctgtgccgca gcgctaaaac taccattacg cgctaccgca tcaacaactt
     5461 cgagtgaata ttctgaccac atagtctgcc tgcaaaattt ttgaaaccaa tcatcaaata
     5521 ttaccgtttc acaacactaa tttcactccc tacactttgc ggcggtgttt aattgagaga
     5581 tttagagaat atacatgcaa cctgggaaaa gatttttagt ctggctggcg ggtttgagcg
     5641 tactcggttt tctggcaacc gatatgtatc tgcctgcttt cgccgccata caggccgacc
     5701 tgcaaacgcc tgcgtctgct gtcagtgcca gccttagtct gttccttgcc ggttttgccg
     5761 cagcccagct tctgtggggg ccgctctccg accgttatgg tcgtaaaccg gtattattaa
     5821 tcggtatgac aatttttgcg ttaggtagtc tggggatgct gtgggtagaa aacgccgcta
     5881 cgctgctggt attgcgtttt gtacaggctg tgggtgtctg cgccgcggcg gttatctggc
     5941 aagcgttagt gacggattat tatccttcac agaaagttaa ccgaattttt gcgaccatca
     6001 taccgctggt gggtctatct ccggccctgg ctcctctgtt aggaagctgg ctgctggtcc
     6061 atttttcctg gcaggcgatt ttcgccaccc tgtttgccat taccgtggtg ctgattctgc
     6121 ctattttctg gctcaaaccc acgacgaagg cccgtaacaa tagtcaggat ggtctgacct
     6181 ttaccgacct gctacgttct aaaacctatc gcggcaacgt gctgatatac gcggcctgtt
     6241 cagccagttt ttttgcatgg ctgaccggtt caccgttcat ccttagtgaa atgggctaca
     6301 gcccggcagt tattggttta agttatgtcc cgcaaactat cgcgtttctg attggtggtt
     6361 atggctgtcg cgccgcgctg cagaaatggc aaggcaagca gttattaccg tggttgctgg
     6421 tgctgtttgc tgtcagcgtc attgcgacct gggctgcggg cttcattagc catgtgtcgc
     6481 tggtcgaaat cctgatccca ttctgtgtga tggcgattgc caatggcgcg atctacccta
     6541 ttgttgtcgc ccaggcgctg cgtcccttcc cacacgcaac tggtcgcgcc gcagcgttgc
     6601 agaacactct acaactgggt ctgtgcttcc tcgcaagtct ggtagtttcc tggcttatta
     6661 gtatcagcac gccattgctc accaccacca gcgtgatgtt atcaacagta gtgctggtcg
     6721 cgctgggtta catgatgcaa cgttgtgaag aagctggctg ccagaatcat ggcaatgccg
     6781 aagtcgctca tagcgaatca cactgatcta tatcgatata cttatactta ggctgctaac
     6841 aaaattttgt tgtatgattg aaattagcgg cctatactaa tttcgagttg ttaaagctac
     6901 gataaatatt atgtttttac ggggacagga tcgttcccga ctcactatgg atggtcattt
     6961 cggcaagggt tcctcctttc cctctgttct acgtcggatt atagactcgc ggttttttct
     7021 gcgagatttc tcacaaagcc caaaaagcgt ctacgctgtt ttaaggttct gatcaccgac
     7081 cagtgatgga gaaactatga gttcatcgtg tatagaagaa gtcagtgtac cggatgacaa
     7141 ctggtaccgt atcgccaacg aattacttag ccgtgccggt atagccatta acggttctgc
     7201 cccggcggat attcgtgtga aaaaccccga tttttttaaa cgcgttctgc aagaaggctc
     7261 tttggggtta ggcgaaagtt atatggatgg ctggtgggaa tgtgaccgac tggatatgtt
     7321 ttttagcaaa gtcttacgcg caggtctcga gaaccaactc ccccatcatt tcaaagacac
     7381 gctgcgtatt gccggcgctc gtctcttcaa tctgcagagt aaaaaacgtg cctggatagt
     7441 cggcaaggag cattacgatt tgggtaatga cttgttcagc cgcatgcttg atcccttcat
     7501 gcaatattcc tgcgcttact ggaaagatgc cgataatctg gaatctgccc agcaggcgaa
     7561 gctcaaaatg atttgtgaaa aattgcagtt aaaacctggg atgcgcgtac tggatattgg
     7621 ctgcggctgg ggcggactgg cacactacat ggcatctaat tatgacgtaa gcgtggtggg
     7681 cgtcaccatt tctgccgaac agcaaaaaat ggctcaggaa cgctgtgaag gcctggatgt
     7741 caccattttg ctgcaagatt atcgtgacct gaacgaccag tttgatcgta ttgtttctgt
     7801 ggggatgttc gagcacgtcg gaccgaaaaa ttacgatacc tattttgcgg tggtggatcg
     7861 taatttgaaa ccggaaggca tattcctgct ccatactatc ggttcgaaaa aaaccgatct
     7921 gaatgttgat ccctggatta ataaatatat ttttccgaac ggttgcctgc cctctgtacg
     7981 ccagattgct cagtccagcg aaccccactt tgtgatggaa gactggcata acttcggtgc
     8041 tgattacgat actacgttga tggcgtggta tgaacgattc ctcgccgcat ggccagaaat
     8101 tgcggataac tacagtgaac gctttaaacg gatgtttacc tattatctga atgcctgcgc
     8161 aggtgctttc cgcgcccgtg atattcagct ctggcaggtc gtgttctcac gcggtgttga
     8221 aaacggcctt cgagtcgctc gctaaaggct attctatcgc cccctctccg ggggcgattt
     8281 cagatcaggc ttctgtacct ggttgattca tggcattttc tcgtgccgcc agcacacgtt
     8341 ctaccgtatc taccactgcc tgagtttgtg gatcaatttc aatgttgacg cgtgcgccga
     8401 gttttttctt cccaagagtc gtgcgttcca gagtttccgg aattaaatgg acgcaaaaac
     8461 gcgttggcgt gacttcgccg acggtcaggc taataccgtc gatgccaata aatcctttgt
     8521 acaggatata tttcatcaac tgactatcct ggactttaaa ccagatctgg cgattatttt
     8581 ccgaggttaa tattttcgcc acttcagcgg tggtcataat atgacctgac attaagtgtc
     8641 cgccaatttc atcgctgaat tttgccgcac gctcaacgtt tacccaatcc cccactttta
     8701 aatcgccaag attggtaatg cgtaacgttt ctttcatcag gtcaaaactg acatggttgc
     8761 cgttaatttc cgtcactgtc aggcagcaac cgttatgcgc cacggaagca cctgtttcca
     8821 ggccgtccag catgtggtcg ggtaactcca ccacatgcgt acgaaaattt ggtttctcgt
     8881 caatcgacac cagttttgcg gtgccctgta caatccccgt aaacatactt acaactcctg
     8941 aaatcagtta agacattctg ttcagcacaa tagcaggtgg aaaacgccct taccagtgaa
     9001 ggggtaagaa tgactatttt ttcactggag aattaataaa tcctcgctac aatagactga
     9061 atttcccctg cttcttcttt ttgctgccca ttcaggcggc tttttagtct ctcatataac
     9121 tacaaataaa aggtgttcac gtgcagaagt atatcagtga agcgcgtctg ttattagcat
     9181 tagcaatccc ggtgattctc gcgcaaatcg cccaaactgc gatgggtttt gtcgataccg
     9241 tgatggcggg cggctatagt gccaccgaca tggcggcggt cgctatcggt acttctatct
     9301 ggcttccggc gatcctcttt ggtcacggac tgctgctggc attaacgccg gttatcgcgc
     9361 aattaaatgg ttccggtcga cgtgagcgca ttgcgcatca ggtgcgacaa ggtttctggc
     9421 tggcaggttt tgtttccgtt ctcattatgc tggtgctgtg gaatgcaggt tacattatcc
     9481 gctccatgga aaacatcgat ccggctctgg cggacaaagc cgtgggttat ctgcgtgcgt
     9541 tgttgtgggg cgcgccaggc tatcttttct tccaggttgc ccgtaaccag tgtgaaggtc
     9601 tggcaaaaac caagccgggt atggtaatgg gctttatcgg cctgctggtg aacattccgg
     9661 tgaactatat ctttatttat ggtcatttcg gtatgcctga gctcggtggc gttggttgtg
     9721 gcgtggctac tgcggcggtg tattgggtta tgttccttgc catggtttct tacattaaac
     9781 gcgcccgctc catgcgcgat atccgtaacg aaaaaggcac cgcaaaaccc gatcctgcgg
     9841 ttatgaaacg actgattcaa ctcggtttgc cgattgcgct ggcactattc tttgaagtga
     9901 cactgtttgc cgtcgtggct ctgttagtgt ctccgctcgg tattgttgat gtcgcaggac
     9961 accagattgc cctgaacttt agttcactaa tgttcgtgct tccaatgtcg ctggcggcag
    10021 cggtaactat ccgcgtaggt tatcgtctgg gtcagggctc aacgctggat gcgcaaaccg
    10081 ctgctcggac cgggcttatg gtgggtgtct gtatggcaac cctgacggcc attttcacgg
    10141 tttcactgcg ggagcaaatc gccctgttgt acaacgacaa tcccgaggtt gtaacgctgg
    10201 ctgcgcattt gatgttgctg gcggcggtat atcagatttc tgactcaatc caggtgattg
    10261 gcagtgggat tttgcgtggt tataaagata cgcgttccat tttctatatc acctttacag
    10321 cttactgggt gctgggcttg ccaagcggct atattctggc actgaccgat ctggtcgttg
    10381 aacctatggg gccagcaggc ttctggatag gctttattat cggcctgaca tcggcagcca
    10441 ttatgatgat gctgcgtatg cggttcctgc aacgtatgcc gtcagccatc attctgcaac
    10501 gagcgtcccg ctaataaaga caaggcgcaa ccttcacggg ttgcgcctgt gtttttacgc
    10561 aggctggagc gttgcgccaa tcccgtcttc gtctggctgt aatttcagag cgttacacag
    10621 agtttgccga ctggcatctg ttatctcaac gcgtatatcc ccgttaactt cccctttcac
    10681 attgatgaag tcaggtgaaa tttctttcgc actggtttgc aggcaaatcg ctccctctcc
    10741 gcgcaggcgt ttcactttca gttgcacatg cgttgccgca ccagtgatca gccacagttt
    10801 tccgccattc agattgatgt taccaacaat gaaagtgacg ctgtcacctt ttaccgtact
    10861 gccgtcgaga atcaacacac cgccctgctg tacagtaatc gttgattgtt gcagctctgc
    10921 gccataacca ataatcattg aagcgccgtc cagtaatgca acactcccgg tcagttcaac
    10981 cggtgcgcga tcttcctcta cgacaaaatt ttcgtccgcg ctggtgggtg acaacaggat
    11041 cactgttcca ccttgtatta ccgcattttc cagcgtaccg gcatgcactt cagcccgccc
    11101 ccccacattc accgacaggt tttgcgtctt accggaactg tagagctgaa ggccatccgt
    11161 ccccagacga taaatggatc cattctcaac aatagtgtca gagtcttcac caccgagatt
    11221 attcatggtg ccaccagcaa gcaacgtatc actggctttc ccggccagca taaaaacgcc
    11281 gccctgatta atctcacaat tgctggcttc tccacttgga tacaccaaca attcaccacc
    11341 ttcatctacc acgaccgctg aggttgtccc attaaccaac aggccgccct cttgatcgag
    11401 aatgattttt tccgcgcgat gtccttccag tacacgcagg ttaccgccat tttccagcaa
    11461 caaaccgcag gcataaccct gatcaacgct gaattcacct tcgggatggc ggccattaac
    11521 cgtagcgagc gtagagagac taattgcgcc tccctcggac tgaacaatat ctgtggcaat
    11581 ggcaccgtcg ctgaccgata gcgtgccatt gttaccgtac tgtgtgcatt tggcgatccc
    11641 accggttgcc actttcaacc agccagcccc cgtgaccacg gtattttcgg ctcttccgcc
    11701 agcttcaaca atcaactcgc cttcagtaag ttgcgtcgcg ccaatgacct cgccggtctt
    11761 aacaatttgc acattcccgt cgctcatcaa gtttttcgca tcagatccca tgatttattc
    11821 ctttgctgca tctgtgtgcc tttattgcta cctaagtgta aaggctacgg aggatttatc
    11881 cacgacagat ttgagatggt ggcaaacaat tctgtttaaa ctctgataca cgaattattg
    11941 ggttgtatca gatgtaaatg cgatcctgaa taaaaatcgc ccttgcaaat caacaaaata
    12001 tagccaattt gcttaaaagc tctgcaaacg gtcgaaatag cgcagaaaat tacgttttgc
    12061 ctcttgccac ctttccactc tgccgctaat atttgtcccc gttgtcacct acaacgttgc
    12121 gttcatagct cagttggtta gagcaccacc ttgacatggt gggggtcgtt ggttcgagtc
    12181 caattgaacg caccatcctg cgtccgtagc tcagttggtt agagcaccac cttgacatgg
    12241 tgggggtcgg tggttcgagt ccactcggac gcaccagatt ttcttaatct ggtcttttcc
    12301 tttttccctc tgtttattct ctgtatccaa tacgttaaaa gatttacact gtcttcgtat
    12361 gcgttatcag aaggagaatc gctatggcaa ctttgttaca acttcatttt gcttttaatg
    12421 gcccatttgg cgacgcaatg gctgagcagc ttaaaccact tgctgagtcg attaatcagg
    12481 aacctggttt tctgtggaag gtatggacag aaagtgaaaa gaaccacgaa gccggtggga
    12541 tctacctttt cactgatgaa aaaagcgccc ttgcctatct ggaaaaacat actgcccgac
    12601 tgaaaaatct cggcgttgag gaagttgtcg ccaaagtttt cgatgtcaat gagccactta
    12661 gtcaaatcaa tcaggcaaaa ctcgcctgac agaatttaat caagggcggt tagcgccctt
    12721 ttcatccctg tctgaaattt ctcaaattct aaaaatctca accaaactta tctgataaca
    12781 ctaaattcga aagaatgcgt acaggtaagt aacaatgaaa aaaattgcta ttgtgggtgc
    12841 cgggcctacg gggatctaca ccttattctc gcttctacag caacaaactc cactttctat
    12901 ttctatcttc gagcaggctg acgaggccgg tgtcgggatg ccatacagtg atgaggaaaa
    12961 ctcaaaaatg atgctggcaa atattgccag tattgaaata ccgccgatta attgtacgta
    13021 tctcgaatgg ctacaaaagc aagaagccag ccatctccag cgttatggcg ttaaaaaaga
    13081 aaccttgcac gatcgtcagt ttttaccgcg aattctgctg ggcgaatatt tccgcgatca
    13141 atttttacga ttagtagacc aggcacgaca gcaaaaattt gcagtggctg tttatgaatc
    13201 atgccaggtt accgatctgc aaattacaaa tgctggcgtc atgctcgcta caaatcagga
    13261 tttacccagc gagacgtttg atttagcggt gattgccacg ggtcacgtct ggcctgatga
    13321 agaagaagca accagaacgt attttcccag cccgtggtca ggcctgatgg aagcaaaggt
    13381 cgatgcgtgt aacgtgggta ttatgggaac atccttgagc ggactggatg cggcaatggc
    13441 agtggctatt cagcatggtt cgttcattga agatgataaa caacacgtcg tttttcaccg
    13501 cgataacgca agtgaaaagc taaatatcac gttgatgtcg cgcacgggta ttttacccga
    13561 agccgatttc tattgcccta ttccctacga gcccttacac atcgtcaccg atcaggcatt
    13621 aaatgctgag attcaaaaag gcgaatatgg ccttttggat cgggtattta gattgatagt
    13681 agaggaaatc aagtttgctg atccagactg gagccaacgc atagccttag agagcctgaa
    13741 tgtcgattcc tttgctcaag cctggtttgc cgagcgcaaa caacgcgacc catttgactg
    13801 ggcagaaaaa aatctccagg aagtcgaacg caataaacga gaaaaacata ctgttccctg
    13861 gcgttatgtc attctgcgcc tgcatgaagc cgtacaggaa attgttccac atctgaatga
    13921 acacgaccat aaacggttca gtaaaggcct tgcccgggtt ttcatcgata attatgcggc
    13981 aatcccttca gagtctattc gtcggctgct ggccttacgt gaagcgggga tcattcatat
    14041 tctcgccctc ggtgaagact acaaaatgga aattaatgag tcgcgcaccg tcctgaaaac
    14101 ggaagacaac agctactcgt ttgacgtttt tattgatgcc cgcggacagc gtccgcttaa
    14161 agtgaaagat attcctttcc ctgggctacg cgagcaatta cagaaaacag gggatgaaat
    14221 ccctgatgtt ggcgaagatt atacgttaca gcaacccgaa gatattcgtg gacgcgtagc
    14281 gttcggcgcg ttgccctggt tgatgcacga ccagcctttc gttcagggac ttacggcatg
    14341 tgcagaaatt ggtgaggcga tggctcgggc ggtcgtaaag cctgcatccc gtgctcgtcg
    14401 gcgtctttcg tttgattaaa ggtaaagcta tcagcactca acaaactgat gctgttcatt
    14461 aagacgatag cggactccgg ttcgaatatt gttttcacct tcaatggcga cggcgaagcg
    14521 aactctttcg ccgtcatgat atgccagtgc ggcgcttccc cctggcccga gaataattga
    14581 gtcgaccacg cccgtgctca ctaccgttga attttcaccg ctggcaatga tgtgtacatt
    14641 gtcgccgctg ttggcaattc tggcgtttgc gccaaagctg gcgatttgca ccaggtcacc
    14701 attactggcg acatgacacc gttcacccaa tgtacaaacg cgtactcgca ttccggtatt
    14761 cgcaatccga ctactatctc cagcgctgct tatccgcgaa gaattaccgg ttacagcaac
    14821 ccgtgcgcgt tcaccggagc tgccaatgtg actgttaaag ccgacacttc caatccgcac
    14881 gctataaccc atactggcaa tttgcgcggc atacccagcg gttacgattc gggcgttatc
    14941 ctcacaacag ccgatgcgag catgatcgtc gctgcgggca aactgactgc aaaatggtat
    15001 ctctccgggg tctgtggcca gtttttgcat agaggaaact tcctgatgcg cgaagttttc
    15061 atccgccagc cattttttcc acgcatactc aaccagactc tccgcccagt ccgtatatcc
    15121 ttcctcaatc agtgcatgat gaatatcggc ataactaccg ccgcgtggga aatggcgcag
    15181 gaaccagcga tacatcaccg ctccgatacg ccattctcgc aggtctgctc gtgtaatgat
    15241 cattcgttag tgccggtgat agccatcgac catgctttta aaggtttcgt gtggcgtacg
    15301 ccccgtggtg caaagataaa gatgtccgaa gataaaaaag aggcttataa atgccagagc
    15361 aaaatgcgcc tgcaataacc agtatcttac gccaggaaac acatctccca cggcttgcgg
    15421 atagagacac aacagccccg tcaatagtaa caacggcagc aatccacaca tgacaccaac
    15481 ataggcgacc tgctgtaagg gattaaattt agactgtgtt gttgccggga aaggatgttc
    15541 ttccccctgc ataatgccaa acaaataaaa tcgtgtttgt tttgccgctc gttccagcca
    15601 cccctgacga cgaatgcgat agtggtgacc attatcccca acggcattga tcagcacaaa
    15661 gccgagccag catgccagta acaaaaatcc gcaaacttca tgcaccgcaa ccagactttt
    15721 aaccacagtt gcgcccacca tcgcaaagtg atttatcagc ccacttgcca gcaacaatac
    15781 aaagagtaac gcattcgacc agtgccataa tctgaccgct ttgctgtaaa gataaacctt
    15841 ctcaccatga tcggtggctg atttcttcac gccacgagca cgaagcaagg cgtgcagccc
    15901 caacaccaac cacatcccaa ccagcaatac tccggcaatg atcaaccaca ccggccaaaa
    15961 ttcgggagtg aactgtggca catagttcgc taactggctc tgaaactgtt cagcatgttg
    16021 cgacgggttc atacattttc ctttttaatc aaatgttgac cgaaccggcg atacagatgc
    16081 ggtttcccgg caccaagtag ctgatattga tagtatttat tttcctgcaa ccacgcctga
    16141 atttctgggc tatcttcgcg accaaagatc aatgcgtgtt ccggacaggc gctgacgcaa
    16201 atgggcggaa agccttttgc cagtcgggac tcagcgcaga aatcacattt atctgccact
    16261 ttagtgacag ggttaaggta gcgaacctgg tacggacaag cgccaatgca ataactgcag
    16321 ccgatgcact gcgatttttc tacccgcacg atcccctgct catcacgcca cgacgcccct
    16381 gtcgggcata cgtcgatgca cggagcatct tcgcagtgct gacatgactg gcgaaaaaag
    16441 tgatactgcg tttcgttgtc gttatcggtg actggaatgt gtgctatcga taaacggctt
    16501 ccctgagcgg ggacgtggtt agttttacgg catgcgcggg cgcaaatatt acagccattg
    16561 caccgtgact cgtcatgaat catggcatac cgaacttcct tttcttgcct cgtgttcgct
    16621 aacagagaag acgcagaacc agtaaaaaag attactgttc ccatccccag aacaaatttg
    16681 cgtcgagtga aggacatctg attatcccct aatgcagtgg ctgcaactgt ccaccctatc
    16741 catgacatct ccctgaaatt tcaggggttc tgaaactgct ctacctcgaa ccattcgcat
    16801 aagccataag ccttgccata ggtcataaat cgctcgctaa acaagtacaa caccgccgtc
    16861 gatgactcca cctgccgtat atcggcatat tcaggtgcaa catccatcgc ggcgatcgcg
    16921 gcttcaatct gcgcttcctg gaagtaatat ggcgcttgca tcagcatggc gactttgtaa
    16981 ggacgaggat acgtctggca ctcaaaacga acggcgtgtg caatggcgcg acaaatgtcc
    17041 tgctccacaa cttgcaaaga catcgccgcg tagttttcac tcatggcctg cgttgagtaa
    17101 tagtaatcat cctgacttcc cgtcatacaa gcgatatccg cgtagtctcc gttctgctta
    17161 atgctttcca gcaaaactga cagttcctct tctgcaaccg aatagggtgg cgagagaaac
    17221 acggtgcgcg ccaccagctg cccagccgca gaatgctggc gaatgaactc agccaggatt
    17281 tcagcaggtt ccggttcggt taccgtatca acggggtgac gcatcccctg caaccattgc
    17341 cgcttcgcct cgtcaacttc gctgactttc gctaacggca attcatcctg atggttcatc
    17401 ttacccattc cttacgcagg cagtagattg tgtgctgcca gatcggcggc aatatcttcc
    17461 agccccagcc gctgcaatgt ttcgcgggtt ggacaaccaa gctgtgggtc ccagcccatt
    17521 tctttgtaga acatcgtgag cgaagcatgc atatcgtcac gatccatttt gtcagtacct
    17581 tcagtaaaga ccgggatctg cggatccttg tcgaaaaccc aggaacagat aagatcgtgt
    17641 tcattacgca tatctttggt ttgcatcagt tttaccgtgt aggcacgatg caacgtaaag
    17701 atacgctctg cagctaaatc taatttttcc tgagtcatct cttcgccggt gatcgctttg
    17761 aagaatttgg cttcaagcgc cagatcgccc cggtaattac ggcttttcag cggcgaaacg
    17821 gtcattggcc agacccagtt gcacagggtg acggcgttat gcaaacagac ccgcaacagc
    17881 gaccactttg catatttaat ttttgcgtca ttgattggcg tgtagttttt ggtttcatcg
    17941 taagcatctt cagaaccaaa aagttctttc gccacttcac gttgcagttt caatggcaag
    18001 ccg
//
LOCUS       DAZOYL010000058        17959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZOYL010000058 DAZOYL010000000
VERSION     DAZOYL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.058"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(229..1203)
                     /gene="cysB"
                     /locus_tag="ACHUOY_001430"
     CDS             complement(229..1203)
                     /gene="cysB"
                     /locus_tag="ACHUOY_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR0682246.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1413..4010)
                     /gene="topA"
                     /locus_tag="ACHUOY_001431"
     CDS             complement(1413..4010)
                     /gene="topA"
                     /locus_tag="ACHUOY_001431"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR0682247.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4390..4641
                     /locus_tag="ACHUOY_001432"
     CDS             4390..4641
                     /locus_tag="ACHUOY_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR0682248.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="ACHUOY_001433"
     CDS             complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="ACHUOY_001433"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR0682249.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5946..6704
                     /locus_tag="ACHUOY_001434"
     CDS             5946..6704
                     /locus_tag="ACHUOY_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR0682250.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6701..7291
                     /gene="cobO"
                     /locus_tag="ACHUOY_001435"
     CDS             6701..7291
                     /gene="cobO"
                     /locus_tag="ACHUOY_001435"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR0682251.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="ACHUOY_001436"
     CDS             complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="ACHUOY_001436"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR0682252.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8416..9530)
                     /locus_tag="ACHUOY_001437"
                     /pseudo
     CDS             complement(8416..9530)
                     /locus_tag="ACHUOY_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9558..10178)
                     /locus_tag="ACHUOY_001438"
     CDS             complement(9558..10178)
                     /locus_tag="ACHUOY_001438"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR0682253.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10175..11056)
                     /gene="rnm"
                     /locus_tag="ACHUOY_001439"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(10175..11056)
                     /gene="rnm"
                     /locus_tag="ACHUOY_001439"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR0682254.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11194..11238
                     /gene="trpL"
                     /locus_tag="ACHUOY_001440"
     CDS             11194..11238
                     /gene="trpL"
                     /locus_tag="ACHUOY_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR0682255.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11215..11308
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11330..12892
                     /gene="trpE"
                     /locus_tag="ACHUOY_001441"
     CDS             11330..12892
                     /gene="trpE"
                     /locus_tag="ACHUOY_001441"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR0682256.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12892..14487
                     /gene="trpD"
                     /locus_tag="ACHUOY_001442"
     CDS             12892..14487
                     /gene="trpD"
                     /locus_tag="ACHUOY_001442"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR0682257.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14488..15849
                     /gene="trpCF"
                     /locus_tag="ACHUOY_001443"
     CDS             14488..15849
                     /gene="trpCF"
                     /locus_tag="ACHUOY_001443"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR0682258.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15861..17054
                     /gene="trpB"
                     /locus_tag="ACHUOY_001444"
     CDS             15861..17054
                     /gene="trpB"
                     /locus_tag="ACHUOY_001444"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR0682259.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17054..17860
                     /gene="trpA"
                     /locus_tag="ACHUOY_001445"
     CDS             17054..17860
                     /gene="trpA"
                     /locus_tag="ACHUOY_001445"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR0682260.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4316 a         4750 c         4487 g         4406 t
ORIGIN      
        1 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
       61 tttgaaaact ggagtttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
      121 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
      181 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
      241 cagttttata tctttaaaca tgacctcaat ttcttcatta gagcgcaatg cgacagccgc
      301 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
      361 gcgcaagaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
      421 agcatcaaca cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
      481 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
      541 gacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
      601 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
      661 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
      721 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagtga aatcagcatt
      781 gcctttagag acggcatcag caatttgtgt cggcgagccc tggtgcatat gcaaagaaac
      841 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
      901 atgcgtggtg gcgatataca gtgagccttt atccggccag gtgtgctctc cggcaaccga
      961 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgccctgc
     1021 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
     1081 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
     1141 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
     1201 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
     1261 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
     1321 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
     1381 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
     1441 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
     1501 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
     1561 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
     1621 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaaaacaccc
     1681 gcagcgccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
     1741 ggcaccggat cttctttcgg tggtgccact tcgccgttac gtaaaatctt acgtgtgttt
     1801 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
     1861 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
     1921 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
     1981 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
     2041 cgcagcgcgt tggtttcagc gtcttcgcct tccagtacgt tcagcacttc gttttccggc
     2101 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcataacc agaacagcca
     2161 aggaataccc cggtgctcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
     2221 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
     2281 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
     2341 tcgtgatttg ccaccaggtc gaggctgttt tccatctgtg cggtgaagtc gtagttcatt
     2401 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgccaatttt ttccgcatag
     2461 aaacgacgat tttctactcg cacgtagcca cgatcctgaa tggtcgaaat gatcgacgca
     2521 taggtagacg gacgaccgat accgcgtttt tccagctctt taaccagcga tgcttcactg
     2581 aaacgggctg gcggcttggt aaagtgctgg gctggtgtaa gttcaacgag cgtcagagca
     2641 tcgcctttat taactgctgg taagatgcga tcttcatcgc ctttacgcaa cgcaggcatc
     2701 acttttgtcc agccatcaaa acgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
     2761 gcaccaatgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggctacgaac
     2821 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
     2881 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
     2941 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
     3001 ccacgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
     3061 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaatcca
     3121 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
     3181 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
     3241 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg atgagtcacc
     3301 tgtaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc ccagaactct
     3361 tccggcacga acgctttaat ttcacgctca cgctcgacca ccaggcgaac cgccaccgac
     3421 tgcacacgac cggcagacag accacgagcg atctttttcc atagcagcgg cgaaaccata
     3481 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
     3541 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
     3601 actcggctat agcgcgcatc atcaccccca atcacttccc gcaggtgcca tgcaatggct
     3661 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
     3721 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
     3781 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
     3841 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
     3901 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag atacttgttg
     3961 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
     4021 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
     4081 taccgatatg gactcgttct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
     4141 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
     4201 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
     4261 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
     4321 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
     4381 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
     4441 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
     4501 cagcgtaatg gcgtgctggt atttatcggc gactactttt tagatgaaca aggattgcca
     4561 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
     4621 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
     4681 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
     4741 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
     4801 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
     4861 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
     4921 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
     4981 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
     5041 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
     5101 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
     5161 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
     5221 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
     5281 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
     5341 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
     5401 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
     5461 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
     5521 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
     5581 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
     5641 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt cgccaaaaac
     5701 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
     5761 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
     5821 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
     5881 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
     5941 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
     6001 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
     6061 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
     6121 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
     6181 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
     6241 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
     6301 acgtcatgca ggtcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
     6361 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttggg cgtcagggac
     6421 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
     6481 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
     6541 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
     6601 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
     6661 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
     6721 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
     6781 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
     6841 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
     6901 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
     6961 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
     7021 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
     7081 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
     7141 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
     7201 tcttgaactg gcggatacgg taagtgagct gcgccccatc aaacatgcgt ttgatgccgg
     7261 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
     7321 catactttga ttaaccgtta ttacgcccgc cagaacgacg accgccgctc acctgactgt
     7381 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
     7441 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
     7501 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
     7561 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
     7621 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
     7681 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
     7741 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
     7801 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
     7861 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
     7921 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
     7981 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
     8041 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
     8101 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
     8161 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
     8221 tcgccttccc aggcgtctga tatgcttttg tgcaacagtg agtcagaact tacatctgac
     8281 tcactgatag taaagtctgt ttgaactctt caggccttaa gtaaacgact cgacgtttta
     8341 actggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     8401 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc caccagcacc
     8461 accaccggaa taacttcgcc ccaacaaact ggttttggcc cccagactca ggtgcatctg
     8521 taatgaaaac aaaacgccac tacgggcaat ttccggggcg ctaacagccg ctgtgagtga
     8581 tggggtgata cgtgctaccc attctcttcc aaggtttgct gagactgccc aacctagcaa
     8641 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgc ccactgtgtg ttgctgcctc
     8701 tttacgcgca agatagttaa cgatagcttc accgtgggca taacgttgct gcccagtttg
     8761 tgtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggaaaacagc
     8821 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatgtagag
     8881 agaatagaag gccacgcctg aacaaatcag tggtaaacaa ttcttccgtc ctcaaggatg
     8941 cggccatttc gatatgtgtc gccctcttct ccagtaaaga gatcgatttc gctaaatgga
     9001 ttacccgcga tatcagggaa cttgagtgaa aatcgggtct tatagatttc aaacggccag
     9061 tggttaccag tcacgttcca gattaacaga tctgaatctc cctcacgcag gaaagcattt
     9121 ttaacctgat aatgaatttg ataatgatag tcgccttgag gcaagaaacg ttgtttatcg
     9181 cccacgacaa tactcattaa cccaatatgc cagtcatgat gccagggttc aggaatacca
     9241 tcacgggtga cgccaacaat atgatagttc agcagtgcag cgtccccacc aggctgcatc
     9301 cagagtcgtg gtaaatcacg ataaaatccg tgtttaatct gctgatttcg cgcctgaaaa
     9361 tgaatatttt ctttgatgtc gagcacccca tccggttgca gagtggcttc aacatcaaag
     9421 agcgaaatcc cttcttcata agaagggatc tccgtgtttt ctgcataact tagagatgat
     9481 acaaatagcc ctgctatcaa cagcaaaatg cagcgaaact tccctgccat agtcttccat
     9541 ccgtggaacg caatttgtta taagaaaggc ttcacatcac ctacgccttc acgcaccacg
     9601 accggcgtat catcggtaag atcaataacc gttgtcggtt tctgcccgag atagccgccg
     9661 tgaataatca aatctacctg tttttccaga cggtctttaa tttcttcggg atcggattcg
     9721 gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc cagtgcttca
     9781 agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt gcgtttttcc
     9841 tgcaacagac ggcgtggcac ttccttcgtc cccttcagga tgaaggtata gttgcccggc
     9901 gtgttgtttt tcattaaacg aaacgcaacg ttatcaacaa atgaataggt cgagagttca
     9961 gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg aatacgacaa
    10021 atacgctcca tcgcgttttt atcttcaatt ttacagccga gcgcatagcc ggaatcagtt
    10081 ggataaacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat cagacgttgc
    10141 tgtgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct gtggtgttct
    10201 gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag agtttacgac
    10261 ctagttcaat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc cataaatgat
    10321 gctgacgcgc aagggccgcc agctgggtac gttcattggg cgattgctgg cactgcgcga
    10381 cttccatcgc gtcaccgtgg tgttcggcaa aatgcgcaac caatcttttc agccatttag
    10441 cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa tgatgaatga
    10501 catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt ttcccgcgcg
    10561 ccagatattt tttaaagaca tccgccattg aactggcttt gccgcacccc acaaggaaac
    10621 gcgcaaaatg accgcgcgtc accgcaccgc cctgcgccag tcgtcgcgcg ccttccagtg
    10681 cccctggtat ttgcgctttt tcaagtcttt cggcaatcag ttgcgcccgc tgattgcgcc
    10741 gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca atattcagcc
    10801 cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg ggaataagat
    10861 tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cacgattgca gctgtgttgt
    10921 catggtcggt gatcgccagg gtaccgacgc gcatctcgac tgcacggtgc accaatactt
    10981 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcgt
    11041 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
    11101 acagttctgg caaatattct gaaatcagct gttgacaatt aatcatcgaa ctagttaact
    11161 agtgcgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt actgaaagct
    11221 tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa tcagataccc
    11281 agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa tgcaaacaca
    11341 aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaacc cgactgcgct
    11401 ttttcaccag ttgtgtggga atcgtccggc aacgctgctg ctggaatccg cagatatcga
    11461 cagcaaagat gatttaaaaa gcctgctact ggtagacagt gcgctgcgca ttacagcatt
    11521 aggtgacact gtcacaattc aggcactttc cggcaacggc gaagccctgc tggcactact
    11581 ggataacgcc ctgcctgcgg gtgtggaaaa tgaacaatca ccaaactgcc gtgtgctgcg
    11641 cttcccccct gtcagtccac tgctggatga agacgcccgc ttatgctccc tttcggtttt
    11701 tgacgctttc cgtttattgc agaatctgtt gaatgtaccg aaggaagaac gagaagcaat
    11761 gttcttcggc ggcctgttct cttatgacct tgtggcggga tttgaagatt taccgcaact
    11821 gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaacgc tgatggtgat
    11881 tgaccatcag aaaaaaaaca cccgcattca ggccagcctg tttgctccga atgaagaaga
    11941 aaaacaacgt ctcactgctc gcctgaacga tcttcgccag cagctgaccg aagccgcgcc
    12001 gccgctgccg gtggtttccg tgccgcatat gcgttgtgaa tgtaatcaga gcgatgaaga
    12061 gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gctggagaaa ttttccaggt
    12121 ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct attacgtgct
    12181 gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt tcaccctgtt
    12241 tggcgcgtcg ccggaaagtt cgctcaagta tgacgccacc agccgccaga ttgagatcta
    12301 cccgattgcc ggaacacgtc cacgcggtcg tcgtgccgat ggctcgctgg acagagacct
    12361 cgacagccgc atcgaactgg aaatgcgtac cgatcataaa gagctttctg aacatctgat
    12421 gctggtggat ctcgcccgta atgatctggc acgcatttgc acccccggca gcagctacgt
    12481 cgccgatctt accaaagttg accgttactc ttacgtgatg cacctggtct cccgcgtggt
    12541 cggtgagctg cgccacgatc tcgacgccct gcacgcttac cgcgcctgta tgaatatggg
    12601 gacgttaagc ggtgcgccga aagtacgcgc catgcagtta attgccgagg cggaaggtcg
    12661 tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcatggcg atctcgacac
    12721 ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gccaccgtgc aagccggtgc
    12781 tggcgtagtc cttgattcta ttccgcagtc ggaagccgac gaaacccgta ataaagcccg
    12841 cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagactttct gatggctgac
    12901 attctgctgc tcgataatat cgactctttt acgtacaacc tggcagatca gttgcgcagc
    12961 aatgggcata atgtggtgat ttaccgcaac catattccgg cgcagacctt aattgaacgc
    13021 ctggcgacga tgagcaatcc gatactgatg ctttctcctg gccccggtgt gccgagcgaa
    13081 gccggttgta tgccggaact cctcacccgc ttgcgtggca agctgcccat tattggcatt
    13141 tgcctcggac atcaggcaat tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa
    13201 attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt tgccggatta
    13261 acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaacat tccggccggt
    13321 ttaaccatca acgcccattt taatggcatg gtgatggcag tacgtcacga tgcggatcgc
    13381 gtttgtggat tccagttcca tccggaatcc attctcacca cccagggcgc tcgcctgctg
    13441 gaacaaacgc tggcctgggc gcagcagaaa ctagagccag ccaacacgct gcaaccgatt
    13501 ctggaaaaac tgtatcaggc gcagacgctt agccaacaag aaagccacca gctgttttca
    13561 gcggtggtgc gtggcgagct gaagccggaa caactggcgg cggcgctggt gagcatgaaa
    13621 attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa ccgcgctact ggaaaacgca
    13681 gcgccgttcc cgcgcccaga ttatctgttt gctgatatcg tcggtactgg cggtgacggc
    13741 agcaacagta tcaatatttc taccgccagt gcgtttgtcg ccgctgcctg tgggctgaaa
    13801 gtggcgaaac acggcaaccg tagcgtctcc agtaaatctg gttcgtccga tctgctggcg
    13861 gcgttcggta ttaatcttga tatgaacgcc gataaatcgc gccaggcgct ggatgagtta
    13921 ggtgtttgtt tcctctttgc gccgaagtat cacaccggat tccgccacgc gatgccggtt
    13981 cgccagcaac tgaaaacccg caccctgttc aatgtgctgg ggccattgat taacccggcg
    14041 catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc gattgccgaa
    14101 accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg gatggatgaa
    14161 gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcgaaat taagagctat
    14221 caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact ggcaggcgga
    14281 acaccggaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg cgacgccgcc
    14341 catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg ccatgaagat
    14401 ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc ttacgacaga
    14461 gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca
    14521 gacaaggcga tttgggtaga aacccgcaaa cagcagcaac cgctggccag ttttcagaat
    14581 gaggttcagc cgagcacgcg acatttttat gatgcgctac agggtgcacg cacggcgttt
    14641 attctggagt gcaagaaagc gtcgccgtca aaaggcgtga tccgtgatga tttcgatccg
    14701 gcacgcattg cctccattta taaacattac gcttcggcaa tttcggtgct gactgatgag
    14761 aaatattttc aggggagctt tgatttcctc cccatcgtca gccaaatcgc cccgcagccg
    14821 attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg ctattaccag
    14881 gccgatgcct gcttattaat gctttcagta ctggatgacg accaatatcg ccagcttgca
    14941 gccgtcgctc acagtctgga gatgggggtg ctgaccgaag tcagtaatga agaggaactg
    15001 gagcgcgcca ttgcattggg ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat
    15061 ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca caacgtgacg
    15121 gtaatcagcg aatccggcat caatacttac gctcaggtgc gcgagttaag ccacttcgct
    15181 aacggttttc tgattggttc ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc
    15241 cgggtgttgc tgggtgagaa taaagtatgt ggtctgacgc gtgggcaaga tgctaaagca
    15301 gcttatgacg cgggcgcgat ttacggtggg ttgattttgg ttgcgacatc accgcgttgc
    15361 gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg
    15421 ttccgcaatc acgatattgc cgatgtggtg gacaaagcta aggtgttatc gctggcggca
    15481 gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga agctctgcca
    15541 gcacacgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc ccgcgagctt
    15601 cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca acgtttcgac
    15661 tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg cttaggcgca
    15721 gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa ttctgctgta
    15781 gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc
    15841 gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt gagtttggcg
    15901 gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca
    15961 gcgcgcaaaa agatcctgaa tttcaggccc agttcaacga cctactgaaa aactatgccg
    16021 ggcgtccaac cgcactgacc aaatgccaga acattacggc tggcacgaac accacgctgt
    16081 atctgaagcg cgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctcggtc
    16141 aggctttact ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc
    16201 agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata
    16261 tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg
    16321 cggaagtgat cccggtgcat agcggttccg cgacgctgaa agatgcctgt aacgaggcgc
    16381 tgcgcgactg gtccggtagt tacgaaaccg cgcactatat gctgggcacc gcagctggcc
    16441 cgcatcctta tccgaccatt gtgcgtgagt ttcagcggat gattggcgaa gaaaccaaag
    16501 cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg
    16561 gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc ggcctgattg
    16621 gtgtggagcc aggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg
    16681 gtcgcgtggg tatctatttc ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga
    16741 ttgaagaatc ttactccatc tccgccggat tggatttccc gtctgtcggc ccacaacacg
    16801 cgtatcttaa cagcactgga cgcgctgatt acgtgtctat taccgatgat gaagcccttg
    16861 aagccttcaa gacgctgtgc ctacacgaag ggatcatccc ggcgctggaa tcctcccacg
    16921 ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga aaaagagcag ctactggtgg
    16981 ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac
    17041 gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga
    17101 aggcgcattc gttcctttcg tcacgctcgg ggatccgggc attgagcagt cattgaaaat
    17161 tatcgatacg ctaattgaag ccggtgctga cgcgctggag ttaggcatcc ccttctccga
    17221 cccactggca gatggcccga cgattcaaaa cgccactctg cgcgcctttg cggcaggtgt
    17281 gactccggca caatgttttg aaatgctggc actgattcgc cagaaacacc cgaccattcc
    17341 cattggcctg ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg agttttatgc
    17401 ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccaa ttgaagagtc
    17461 cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca cctatcttca tctgcccgcc
    17521 aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt acacctattt
    17581 gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc tcaatcatct
    17641 ggttgcgaag ctgaaagagt acaacgctgc acctccattg caggggtttg gtatttccac
    17701 cccggatcag gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt ctggttcagc
    17761 cattgttaaa atcatcgagc aacatattaa tgagccagag aaaatgctgg cggcactgaa
    17821 agtttttgta caaccgatga aagtggcgac gcgcagttaa tccccctgcc gccagttccg
    17881 ctggcggcat tttaactttc tttaacgaag ccggaaaaat cctaaattca tttaatattt
    17941 atctttttac cgtttcgct
//
LOCUS       DAZOYL010000059        17937 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZOYL010000059 DAZOYL010000000
VERSION     DAZOYL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.059"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            157..462
                     /locus_tag="ACHUOY_001446"
     CDS             157..462
                     /locus_tag="ACHUOY_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0682261.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            569..1213
                     /gene="gfcB"
                     /locus_tag="ACHUOY_001447"
     CDS             569..1213
                     /gene="gfcB"
                     /locus_tag="ACHUOY_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR0682262.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1210..1956
                     /locus_tag="ACHUOY_001448"
     CDS             1210..1956
                     /locus_tag="ACHUOY_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0682263.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            1956..4052
                     /locus_tag="ACHUOY_001449"
     CDS             1956..4052
                     /locus_tag="ACHUOY_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0682264.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4098..5237
                     /locus_tag="ACHUOY_001450"
     CDS             4098..5237
                     /locus_tag="ACHUOY_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR0682265.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5225..5671
                     /gene="etp"
                     /locus_tag="ACHUOY_001451"
                     /gene_synonym="yccY"
     CDS             5225..5671
                     /gene="etp"
                     /locus_tag="ACHUOY_001451"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR0682266.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5691..7871
                     /gene="etk"
                     /locus_tag="ACHUOY_001452"
     CDS             5691..7871
                     /gene="etk"
                     /locus_tag="ACHUOY_001452"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR0682267.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(7986..9284)
                     /gene="appA"
                     /locus_tag="ACHUOY_001453"
     CDS             complement(7986..9284)
                     /gene="appA"
                     /locus_tag="ACHUOY_001453"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR0682268.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHSVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9364..9456)
                     /gene="cbdX"
                     /locus_tag="ACHUOY_001454"
     CDS             complement(9364..9456)
                     /gene="cbdX"
                     /locus_tag="ACHUOY_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR0682269.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9469..10605)
                     /gene="appB"
                     /locus_tag="ACHUOY_001455"
     CDS             complement(9469..10605)
                     /gene="appB"
                     /locus_tag="ACHUOY_001455"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0682270.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10617..12161)
                     /gene="appC"
                     /locus_tag="ACHUOY_001456"
     CDS             complement(10617..12161)
                     /gene="appC"
                     /locus_tag="ACHUOY_001456"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR0682271.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12295..13152)
                     /locus_tag="ACHUOY_001457"
     CDS             complement(12295..13152)
                     /locus_tag="ACHUOY_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR0682272.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13209..13547)
                     /gene="hyaE"
                     /locus_tag="ACHUOY_001458"
     CDS             complement(13209..13547)
                     /gene="hyaE"
                     /locus_tag="ACHUOY_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR0682273.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(13544..14131)
                     /gene="hyaD"
                     /locus_tag="ACHUOY_001459"
     CDS             complement(13544..14131)
                     /gene="hyaD"
                     /locus_tag="ACHUOY_001459"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR0682274.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14128..14834)
                     /gene="hyaC"
                     /locus_tag="ACHUOY_001460"
                     /pseudo
     CDS             complement(14128..14834)
                     /gene="hyaC"
                     /locus_tag="ACHUOY_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(14853..16646)
                     /gene="hyaB"
                     /locus_tag="ACHUOY_001461"
     CDS             complement(14853..16646)
                     /gene="hyaB"
                     /locus_tag="ACHUOY_001461"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR0682275.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16643..17761)
                     /gene="hyaA"
                     /locus_tag="ACHUOY_001462"
     CDS             complement(16643..17761)
                     /gene="hyaA"
                     /locus_tag="ACHUOY_001462"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR0682276.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4372 a         4795 c         4680 g         4090 t
ORIGIN      
        1 cttatatttc cctctgcgca cctttcctac ccgcagtatc agtatatagt gcaagcggta
       61 tgaggaaagg gagatttcac aaggatgtta tactcaaagt agacaatcct gatctcaagc
      121 gtacgtattg tccatgcgat gaataaagga aaagttatga aacacaagct ttctgcaatc
      181 cttatggcct tcatgttgac gacgccagct gcgtttgctg cctctgaagc aacaaacggg
      241 accgaggcaa cgacgggtac tactggcacc acaacgacca ctaccggcgc aaccacgact
      301 gctactacca ctggtggcgt ggctgctggc gctgtcggca cggcgacagt tgtcggtgtg
      361 gcaacggctg ttggtgtggc gacgttggca gttgttgcag caaatgacag tggcgatggc
      421 ggttctcata acacctcaac caccacgtcc actacacgct aatactgctt taacgcgtcg
      481 aaattaaagt taaatgatac ccggttgcga tattcgtacc gggctttaga gatttgcatt
      541 tttttaatta acctttcaag gattaaaggt gcgccctctt attttatcga ttttcgcact
      601 atttcttgcg ggatgtacgc acagccagca aagtatggtc gatacatttc gcgccagcct
      661 tttcgataat caggatatca ccgtagcgga tcagcagatc caggcgttgc cttattccac
      721 gatgtattta cgccttaatg aagggcaacg aatctttgtg gtactgggat atatagaaca
      781 agaacaaagc aaatggttat cccaggataa cgccatgctg gttacccaca atggacgtct
      841 tttaaaaacc gtcaaactta ataataatct gctggaagtg acaaattccg ggcaggaccc
      901 tctgcgtaac gcgctggcaa taaaagatgg cagccgctgg acgcgcgata ttctctggag
      961 tgaagacaac cattttcgct ctgcgaccct gagttctact ttttcctttg ctggattaga
     1021 gacgctgaat attgcgggtc gcaatgtgct gtgtaatgtc tggcaggaag aggtgacttc
     1081 cacgcggcca gaaaaacagt ggcaaaacac attctgggtc gattcggcta ctggccaggt
     1141 tcgtcaaagt cggcaaatgt taggcgcagg ggttattccc gtagaaatga cgtttcttaa
     1201 acccgcacca tgaataaatt acagtcgtat ttcattgcca gcgtacttta cgtaatgaca
     1261 ccccatgcct ttgcgcaagg aacggtgact atttatctgc ctggcgaaca acagacactt
     1321 tccgttggcc ccatggaaaa tgttgtccag ttggtgacac aaccgcaact gcgcgatcgc
     1381 ctctggtggc ctggtgcctt gctgaccgac tctgcggcga aagctaaagc gcttaaagac
     1441 taccaacatg tcatggcaca gcttgcttcc tgggaagcag aagctgacga cgatgtggcg
     1501 gcaacgatta aatccgtgcg ccagcagtta ctcaatctca atatcactgg tcgcttaccg
     1561 gtcaaactgg accctgattt tgtacgagtg gatgagaaca gcaatccccc gttggtcggg
     1621 gactatacgc tctataccgt acagcgtccg gtgactatta ctctgctggg tgcggtttct
     1681 ggggccgggc aattgccctg gcaggcaggc cgtagcgtta ccgattatct gcaagatcac
     1741 ccccgccttg caggcgcgga caagaataac gttatggtga ttacgcctga gggtgagact
     1801 gtcgtcgccc ctgttgccct gtggaataaa agacatgtgg aaccaccgcc tggcagccag
     1861 ctttggttgg gcttttctgc gcatgtactg cccgaaaaat atgctgattt aaatgaccag
     1921 attgtgtctg ttttaacgca acgtgttcct gactgatgaa gaagaattct tatcttttaa
     1981 gctgcctggc cattgccgtc tccagtgcct gtcatgctga agtattaacc tacccggatc
     2041 cgctgggttc gtcgcaatca gactttggcg gcacaggatt gttgcagatg ccaaatgcgc
     2101 gcatcgcacc ggaaggtgaa ttcagcgtca actaccggga taacgatcaa taccggttct
     2161 actccacctc cgtggcgctg ttcccatggc tggaaggcac cattcgttat acggatgtgc
     2221 gcacacgcaa atatagccag tgggaagatt tcagcggcga tcagtcatac aaagacaaat
     2281 cattcgattt taaacttcgc ctgtgggaag aaggttactg gctaccgcaa gtggcgtttg
     2341 gtaaacgtga tattgctggt acgggtctgt ttgacggtga gtatctggtg gccagcaagc
     2401 aagcggggcc atttgatttc accctcggga tggcatgggg ctacgccggt aatgcgggca
     2461 atattaccaa cccgttttgc cgggtgagcg ataaatattg tcatcgcgca gagtctcacg
     2521 atgcgggcga tatcagcttt agcgatatct ttcgtggccc ggcttccatc tttggcggca
     2581 ttgagtatca aacgccgtgg aatcccctgc gtctgaaact cgaatacgat ggcaacaatt
     2641 accagaatga tttcgctggc aaactgcctc aggcaagcca tttcaacgtc ggcgcagttt
     2701 atcgcgctgc cagctgggca gatctcaacc tgagttatga acgcggtaac acgttgatgt
     2761 ttggcttcac gttacggacc aatttcaacg atctgcgccc tgccctgcgc gatacgccaa
     2821 aaccggcata tcaacctgcg cctgaatctg aaggattgca gtacaccaca gtagcgaacc
     2881 aacttaccgc gctgaagtac aacgcaggtt ttgaagcacc ggaaattcag ctgcgcgata
     2941 agacgctgta tatgtctggt caacaataca aataccgtga ttctcgcgaa gcggtcgatc
     3001 gtgccaaccg gattctggtg aataacctgc cgcaaggcgt tgagaagatt agcgtgacgc
     3061 aaaagcgcga gcatatggcg atggtgacta ccgaaaccga cgtagccagc ctgcgcaaac
     3121 agctggctgg tacagcgcct ggtcaatcag agcaacttca acaacaacgt gttgaagcag
     3181 aagatctttc tgcctttggt cggggctacc gtattcgtga agatcgcttt agctactctt
     3241 tcaacccaac actttcacag tcgctgggcg gcccggaaga tttctatatg ttccagctgg
     3301 ggctgatgtc cagtgcccgc tactggttta ccgaccacct gctgcttgat ggcggtattt
     3361 tcaccaatat ttacaacaac tacgacaagt ttaagtcttc gctgttgccc gcggactcta
     3421 ccctgccccg cgtgcgcacg catatccgtg attacgttcg caatgacgtt tatctcaaca
     3481 acttgcaggc gaactacttt gccgacttag gcaatggttt ctatggccag gtgtatggcg
     3541 gttatctgga aacgatgtac gccggtgtcg gttccgagct gctttatcgc ccgctagatg
     3601 ccagctgggc gctgggtgtg gacgttaact acgtgaagca acgtgactgg gacaacatga
     3661 tgcgcttcac cgattattcc acgccaactg gtttcgtgac ggcttactgg aacccgccga
     3721 cgctcaatgg cgtactgatg aaacttagcg ttgggcaata tctggcaaaa gataaagggg
     3781 caacgatcga cgtcgccaaa cgctttgaca gcggcgtggc ggtaggggta tgggcggcaa
     3841 tcagtaacgt atctaaagat gactacggcg aaggcggctt tagtaaaggt ttttatatct
     3901 cgattccgtt cgacttgatg accattggac ctaaccgcaa ccgcgcggtg gtttcgtgga
     3961 caccattgac gcgtgatggt ggacaaatgc tgtcacgcaa ataccagctc tatccaatga
     4021 cggcagagcg agaagtaccg gttggacaat aacctgacgt aattcataac attacattga
     4081 ctgataaata taaaccaatg aaaaagaaca tttttaagtt ttcggtactg acattggctg
     4141 ttctgtcttt gacagcatgt acccttgttc ctggtcagaa tttatcgacc agtaacaagg
     4201 atgtcattga actacctgac aatcagtatg acctggacaa aatggtcaat atctatcccg
     4261 tcacgccagg attgattgat cagttgcgcg ccaaaccgat tatgtcgcag gctaacccgg
     4321 agctggagca gcaaatcgca aactatgaat atcgtatcgg gattggtgat gtgttgatgg
     4381 ttacggtctg ggatcacccg gaactaacca cccccgcagg gcaataccgt agcgccagtg
     4441 ataccggcaa ctgggttaac gccgatggtg cgattttcta cccctatatt ggtcgcctga
     4501 aagtggccgg gaaaacactt acccaggtac gcaacgagat tacagcgcgt ctcgattcag
     4561 tcattgaaag cccgcaagtc gatgtcagcg tcgcggcgtt ccgatcgcaa aaagcgtacg
     4621 tcaccggcga agtcagcaaa tcgggtcagc agccgattac caatattcct ctgaccatca
     4681 tggatgcgat taacgccgcg ggcggtctga ccgcagatgc agactggcgc aacgtggtgc
     4741 tcacccagaa tggcgtaaag accaaagtga acctctatgc gctgatgcag cgtggcgact
     4801 tgcgccagaa caaactgctg cacccgggcg atattttatt tattccgcgc aacgatgatc
     4861 tgaaagtctt cgtgatgggt gaagtgggta aacagagcac gctgaaaatg gaccgcagcg
     4921 gtatgaccct ggcggaagcg ctgggtaacg ctgagggtat gaatcaggat gtcgctgatg
     4981 cgacggggat ttttgttatt cgtgcaacgc aaaacaagca gaatggcaaa atcgccaata
     5041 tttatcagct gaatgcgaag gacgcatcgg caatgatact cggaacggaa ttccagcttg
     5101 aaccttacga cattgtttat gtcactaccg cgccgctggc tcgttggaac cgtgtgattt
     5161 ctctgctggt accgacgatt tccggggtgc atgacctgac agagacttca cggtggattc
     5221 aaacatggcc caactaaaat ttaactcaat cctggtggtt tgtaccggca acatttgccg
     5281 ttcgcccatt ggcgagcgct tgctgcgtaa acgtctgccg ggcgtaaaag tcaaatcggc
     5341 aggtgttcat ggtctgataa aacaccctgc agatgcgaca gcggcagatg ttgccgctaa
     5401 tcatggcgta tctcttgaag gacatgccgg acgtaagctc actgctgaga tggcacgaaa
     5461 ttacgatctg attctggcaa tggaatcgga acatattgct caggttaccg caattgctcc
     5521 cgaggtccgc gggaagacaa tgctgtttgg gcaatggctg gaacagaaag agatcccgga
     5581 tccctatcgt aaaagtcagg acgcatttga acatgtctac ggtatgttgg agcgcgccag
     5641 tcaggaatgg gcgaagcgcc tcagccgata atttgagttt ataaaatacg atgacaacta
     5701 aaaatatgaa tacgccacca ggcagcactc aggaaaatga gatcgatctg cttcgtctgg
     5761 tcggcgagtt atgggatcac cgtaagttta ttatcagcgt gaccgcgtta ttcacgctga
     5821 tcgctgtcgc ttactcgctg ttaagcacac caatttatca ggcagatact ctggtccagg
     5881 ttgagcaaaa acagggcaac gccattctca gcggcctgag cgatatgatc cctaactcat
     5941 cgcccgagtc tgcaccggag atccaactgc tgcaatcgcg catgattctc ggtaaaacca
     6001 ttgctgaact gaatctgcgc gacatagttg agcagaagta ttttccgatt gtgggtcgcg
     6061 gctgggcgag attaaccaaa gaaaaaccag gtgagctggc gatcagctgg atgcatattc
     6121 cacaactgaa tggtcaggat cagcaactga cactcacggt tggggaaaac ggccactata
     6181 cactggaagg tgaagagttc accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg
     6241 gcgttgcgct gactatcgcg gacattaagg ccaaaccagg aacacagttt gtcctgagcc
     6301 agcgtaccga actggaagcg attaacgcat tgcaggaaac ctttaccgtt agcgaacgca
     6361 gtaaagaaag cgggatgctg gaacttacca tgactggtga tgatccccag ttgattactc
     6421 gtattctgaa cagcatcgct aacaactatt tgcaacagaa tatcgctcgc caggcggcgc
     6481 aggattcaca aagccttgaa ttcttacagc gccagttacc tgaagtgcgc agcgagctgg
     6541 accaggcgga agaaaaactc aacgtttatc gccagcagcg cgattcggtt gaccttaacc
     6601 tggaagccaa agccgttctt gagcagattg tgaacgttga taatcaactc aatgagctga
     6661 ccttccgcga ggcagagatc tcccagctgt ataagaaaga tcacccaact tatcgtgcgc
     6721 tgctggaaaa acgccagacg ctggagcaag aacgcaaacg cctgaataag cgggtatcgg
     6781 caatgccttc cacccaacag gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg
     6841 cggtatatct gcaattactt aaccgccagc aggagttgag tatttcgaaa tccagtgcca
     6901 ttggtaacgt gcggattatc gacccggcag tcactcagcc gcagccagtg aaaccgaaaa
     6961 aagcgttgaa tgtggtgctt ggttttattc ttggcctgtt tatttctgtg ggtgccgtgc
     7021 tggcgcgtgc gatgttgcgt cgtggtgtag aagccccgga acaactggaa gagcacggca
     7081 tcagcgttta tgccactatc ccaatgtccg agtggctgga taaacgcacc cgtctgcgta
     7141 agaaaaattt attttctaat cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg
     7201 tggataaccc ggcggattct gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt
     7261 tcgctatgat ggagacggag aataacattc tgatgatcac cggtgcgacg ccagacagtg
     7321 gtaaaacgtt tgtcagttca actctggcag cggtgatcgc ccagtccgat caaaaagtgt
     7381 tatttattga tgccgactta cgccgtggtt attcgcataa cctgtttacc gtgagtaatg
     7441 aacatggctt gtcggaatat ctggcaggta aagatgagct caacaaagtg atccagcatt
     7501 ttggcaaagg aggctttgat gtgattactc gcggtcaggt gccacctaac ccgtctgaac
     7561 tgctgatgcg cgatcggatg cgtcaattac tggaatgggc gaacgaccat tacgatctgg
     7621 tgattgtcga tacgccgccg atgctggcgg tgagtgatgc cgcggtcgtg gggcgttctg
     7681 ttggcaccag cctgctggtt gcgcgttttg gcttgaacac cgccaaagag gtgagtttgt
     7741 caatgcagcg tctggaacag gcaggcgtca atattaaagg cgctatcctc aatggtgtga
     7801 ttaaacgcgc cagcaccgct tacagttacg gctataacta ttacggttat agttactccg
     7861 agaaagagta aaactgaaag tggccggatg cggcgtgaac gccttttccg gtctacgcat
     7921 tagacagttc ttcgtttgtc atcagcctca gagcattcag gtaactgaat gctctttttt
     7981 atgcattaca aactgcacgc cggtatgcgt gcttcattca cgatttgcgt aaaacctgcc
     8041 aacgaacaca tgccctgcgc atttcgctct tcacatcctg ccagggtcag tttcacctct
     8101 ccgggcggcg tatttaatga cagcggcgtt ttatcacgca tctgctgtaa agtctggaag
     8161 accagcgaaa cctgaatcca ctggctgtta tcgcttagcc gacgccagcg ttcaaacacc
     8221 agttcaccac ctggcggcgt gttatccggc tgaccgggaa gcgtccagtt gagctccagt
     8281 gcgccgccga gatttgccag attagtatcg tgtccggcga taaacagcac tgaagtgggt
     8341 aatgtcacac catacgcctg tttttgcggt ggatggggcg tcaacgctgt cttgatcaaa
     8401 tctaataacg gggtggcgcg gctgcgggca acctctggcg tgcgttgtag caaataaaat
     8461 tgcgcgttat gcaaacttag caaggtgttc cactggtgtg aatcggtgat ccttccccac
     8521 cccggctccg gcattccctg tgcttgttgc aggagaaata tctccgtcag cattgatgcg
     8581 aggcttaccg caccggttaa tgagacattg tcggcgctca ccttgagttc cgatggtaat
     8641 gcctgcgtta atgaacagct ttcgtcctgt ttctcacgtt taaggcacaa gtttgattgc
     8701 ggaaaattaa gcacccgttc cagttcgcga aacgccgttt gccgatgccc ggtaaagtca
     8761 gcaattgacc ctcctgccct gctgaggatc gcgtcagtca cgttcgcgtt atccagttgg
     8821 caaacgccag tttttagagg attaaataac ggatcgggac tggacgtatc agcctgggta
     8881 tgtacggtta ttgcacagtc aggtgccagc ccggcggcga aggcttcgcc tgttttacgg
     8941 gtacgctcgt cgacatcagc aataatcgcg acctgaccag actgcgggca gccctttttc
     9001 gccagcaatc cgtcggctac cagacgctgg cgttggtaat gtccgagata ggcgattagc
     9061 tcaccaccgc gcggtgtcag ccaacccagt tttaccggcc aggttggcca tgcgtctggg
     9121 gtgacatcct gcatcagttg cgtggccttg gttggagcac gcacactatg acgactgaca
     9181 atcaccacac tttccagctt cagctccggc tcactctgag cgaatgcaga ttgcggggtt
     9241 aacggaatca gaagagataa aaatgggatt aagatcgctt tcatcgatat gtttccgctt
     9301 ttcatatctg acattattga ttgcctgatg cgatgctaat gcgccgcatc aggcctacgt
     9361 tcgttaactt ttcagacgcg ggtccagcca taccaacaca agggtggaga gcgaacacat
     9421 caacaaaatg ccgacgaacc aaagtaaata ccacattgtt cctgctcctt agtacaactc
     9481 gttttcgtta cggcggagag tttctgttgt catgcgtccc cacattttgt agtagctcca
     9541 gagagtgtag agcaacacaa tgggcaaaaa tatcagcacg attaccaaca taatgctcag
     9601 cgtcagctga ctggaagtgc tgtcccacaa cgtcaggctg gagattggac tcacgcttga
     9661 cggcatgaca aaggggaaca gcgtgatgcc tgccgtgaaa atcacgccaa attgcatcaa
     9721 tgacgccatt aaaaatcccc aacccgggcg accacgataa atcgccatca ccgtcagcaa
     9781 tgggcagaag aaccccagca gcgggaagat ccacaaaacg ggcgattcga cgaaattatt
     9841 catccaggca ccaggtagca ctgccaccag tttcattaac ggattggaag gaccgttagc
     9901 atcctgggcg agcagtacaa agccatcaat accgacccac agccagtaac ccgccagcaa
     9961 aaagcacagc atcaccaaca gtgcagcgcg tttggtggcc agctgtgaac gcagatgaat
    10021 cacaccaaca gttttcagtt gtaaccagac gccaccttgc aaaatcacca tcccaaggct
    10081 gagcaatccg cacaataaag ggaatggcgt cagcagttgc cagaagctgc cgagatactc
    10141 cacgcgtaat tgcggtgtga aggcaaacgg cacgccgagc aacaagttgc cgaaggcgat
    10201 accgaagact accggcggca ccagactgcc gatgaccaga ccggcgtccc acattttacg
    10261 ccagcgtgca tcggcgattt ttccgcgata atcaaaggcc agcgggcgga agaacagtga
    10321 gcacagcacc aggatcatcg ccacataaaa gccggaaaac gccgctgcat acactctggg
    10381 ccaggcggca aataatgccc caccagcgag gatcaaccag acctggttgc cttcccagtg
    10441 tgcaccaacg ctgtttatca ctatccggcg ttcatcatca ttacgcgcca ccagcggcag
    10501 cagacagccg atccccatgt caaatccgtc ggagatcata aagaccacca ggatcacgcc
    10561 aatcagcagc caccagatga agcgcaatgt ttcataatca aacatgattt tcccctttac
    10621 ccctgttgct gcgtcggttg ttcactctgc atcgcgctcg gccccagacg ggcatatttc
    10681 tgcatcaggt agacttcggc gattaagaac agggtgtaaa gccctacgat catgatcagt
    10741 gagaaagcca gttgtcctgt ggttaaagcg gagtgcgcgg agtatgtcgg taagatgtcc
    10801 tgtatcgccc acggctgacg accaaactcg gtcataaacc acccggcttc aatcgcaatc
    10861 cacggcaacg gcaaactcca gagcgccatt ttcagcaccc agcgatgctg gtcgattttg
    10921 ccacgcagcg tctggacaag cgcaatcagc atcaccagta gcggcaggga accacagccc
    10981 accatgatgc ggaaactcca gaataccggc gcaacctgag gtatcgcgcc acgcatcgcc
    11041 gcctggtact gtgcggctgt gacatgattc atatccggcg catagcggga gagcaacatg
    11101 ccgtagccca ggtcaccttc cagttcccgg aatgcctgca acacatgcgg ctcacgattg
    11161 ccttgcgata tttcctgcat taacagccag gccatacgtc cgcgttgcaa gcgtgggtag
    11221 gtttcagcca tcaaattttt cagacccggc acggttttat ctaatgagtg agtggcgagg
    11281 atccctaaca gcgcgggaat tttgatggca aaggcgttac gctcttgatc ctgttccggc
    11341 caggcaacca catggaacgg tgcaggagca ggttccgttt gccactcccc ttccatcgcc
    11401 gccagtttta ccggttgtac ttgcgcgact tcatacgcag aactgtctcc gagttgtagc
    11461 gtgccgataa tcgccagagt accgaagacg gaaccgatgg caaacgagcg taatgcaaca
    11521 tcgcgctccc gtccgcgcag taaataccag gcgctgatcg ccataataaa catggccccg
    11581 gtcacgtagc ccgccattac ggtgtgcaca aatttcacct ggctgaccgg gttaaagacc
    11641 agctcgctga agctggtcat ctccatacgt agggtgtcga tatcaaaatg cgcaccggtc
    11701 gggtattgca tccaaccgtt ggcattcaat atccacaatg cagagagatt tgaaccgaac
    11761 gccaccagcc acgtcaccag caggtgctgg tatttattca gccgctgcca gccgaagaag
    11821 aaaagcccga caaaggtgga ttcgaggaag aaggccatta atgcttccat cgccagcggt
    11881 gcgccaaaaa tatcgcccac atagttggaa tagaatgacc agttagtacc aaactgaaac
    11941 tccatggtca ggccggtagc cacgccaaga gcaaagttga taccgaagag cttaccccag
    12001 aagcgcgtca tatcgcggta gattgttttg ccggtgacca cgtaaatggt ttccatgata
    12061 gccagcaaaa aaatcagccc cagggtaagg ggtacaaata aaaagtgata cagcgcggtc
    12121 agagcaaact gccagcgcga taaatcaatg acatcccaca tgcgcactcc tgtaggcgaa
    12181 gagagaaaaa taacccgata atgttagatc tccctcttta tcctgaaact ctacgaccag
    12241 aggtattcgc cgattgcgat attcataaga ctgaaagcga tacttaccgt ctttttacgt
    12301 cggtgcagct tccgccagcc actgacatac ctcgctaagc cgctgcgcag agtcgaccaa
    12361 atcttctggc gctgccagca ccacttccgg tattgggcag atttcataac tttccagtaa
    12421 aggacctttt aaggtgtccg tacagcgtaa atgccagaca tggcgtaacc ccgtggcgtt
    12481 gatatagctc tcgccatagc caatggtacg aatctgaata tttcccggcc cacagagacg
    12541 tgagagaaaa agccgatcag cctcgctgat gggtaattgc gtcagattaa tgctgtgcgg
    12601 ctgcgcgtcg gggttacgta catgcgccag taactcatgc gccaacggta ggccattcat
    12661 caggccatca atgggcggcg gtaacagcga atctgtcggc aagagatttt gcgtggccgc
    12721 ctgccacaac gccagcggcg cgcagccggc ctccagtttg tcctccagca acttttcgcc
    12781 gcgacgtttg cgcacccgcc ataatccgca gaagatcgcc tcctgtattt cactttcgct
    12841 gtcgtcagcc tgctgaatgc gcactgacac ttccccttcc ccgagaagcg tattgataaa
    12901 gtgacgatcg tcggcgttga gatttaacag atcgactgcc agcacctcgc ccaacggcgg
    12961 ctggcgttga cttagttgat gttgtaactc gtttaacagc gcaatcacct gcgggctgtg
    13021 agcacattgc tccagggccg ccatactcgg ttcatcattc acctgacagc tgatcggcag
    13081 tggattcatg ctgaaactgt catcgttcgg ttgcgttcct ggccccagca gatggaaaaa
    13141 agtttcgctc atgaggcacg ctcctgttgt ggttcgacaa gcccacgcat caggtttatc
    13201 agttccgctc acgggtgaat accattcagc acaccgcgat agtttccgcc ggtaaacact
    13261 aatgtggcgg gaaagcgaaa gacgccaaag cgatctccga tggcttcgct ctgttcaagg
    13321 tcagcaatcg ccacctgcca tgtgtagccg ggaaactcgc gcagtaattc gccaatcatt
    13381 accggattat cgctgacctc tggcgtgcgt ttcgggtcac tgcttaataa caccacgccg
    13441 tctggcgctt gcgtaagcca gtcgtcaaga cggaattcac taaccggcgt ccagccgcgc
    13501 gccagcattc gttgccacaa cgcatcaaat ggcgtgtcgt tgctcatccc tgctcctcca
    13561 gtgtcatcct gtactggcgc aagcgaacgc cttcgtaatt ttccatcgac agacagtcat
    13621 aattgagaca gcgcgattca ttagccggtt gcggcacaat tccccatgca gccagctgcg
    13681 ccagcgccgc ctgttccgct gcgggcagtt gctcccgtgc cagttcgctc aggctgccgc
    13741 cgtagtcgtc gagcattgcg ggttgcagac cgacgagggc aatatgtgct ggcagatgtc
    13801 cgcggatatc tgccagcgcc agcacttcgg agaaactgtt ctggtgcagg ctcattttct
    13861 tcgcgctgag ataagccgga atacgttctc cggcataggt tcgcagcgtt ccaggttcca
    13921 gcccgtagtc aatggcatcg agaatcaaca gatggctggc gctttcgaca taccccagca
    13981 agttcagtcc ctgagtaccg ccatcgacaa tctccacata ctcgggccag tggtaatggg
    14041 catacaaccg ttccgccacc cgcacgccga aaccttcatc ggcccacagc aggttgccca
    14101 gccccattac cacaacgcgt tgctcgctca tgaacgctcc ttgttactta ttttgccaaa
    14161 tttgtggcta cggtagccgt tgaccatggt ggagatcacc gtgtcgtcgg acatgatgtc
    14221 ttcacgcagc gccatataga catgaccgat cacaaacgcg ccaatcagcc acatccccag
    14281 ccgatgccag ctgtgaatgt ccattgagtt gcctcccgtc cagtagaaaa attccaccac
    14341 ataacggaac ggcgcaaaaa tagcgtactg gctgtgttcg ctgtacagcg caaagccggt
    14401 gatgatcata aagaccgaca tcaggaaata gccgaacatc gccgcctggg cgatggggtt
    14461 atggcctata tcggcactcg gacgttttgc cagaacagat accagcggat ttcataccac
    14521 acgccctgcc accagctttt acgccatacc ggcacgataa acagctcgcg ggagtatcga
    14581 ttgccaacaa aagcccagta gatccgcatc agcaagacca cggtaaaaac catcccggcg
    14641 ctgaagtgaa ttaacctgat gtagcccata tagaacagat acgtcgcctc gccgctgacg
    14701 gaaggtagcg gcttgccgat aaagtatccg gtgaccatca acaccgccat gcataacacc
    14761 gtcagccagt gccagatgcg gactggcgct tcaaagacat agtggctgac aacgttgtcg
    14821 cttttctgtt gcatgatgat tctccttcgc tgttaacgca cctgcacgga gatcagctcg
    14881 ctaccgtcgt cgcccagcac gtgtgttgaa caggcgaggc acgggtcaaa gctgtgcaga
    14941 gtacgcagga tctccagcgg ttgctcgggg atcgccattt tggtgttcat cagcgccgct
    15001 tcataagcgc caatctgccc tttgggatcg cgcgggctgg tgttccaggt ggtcggcacc
    15061 acgcactggt agagatcaat cttgccatcg cgaatggcgg cccagtggcc taacgccccg
    15121 cgcggcgctt cggtaaagcc gacgccacgg cactctgtcg gccaggttgc aggttcccat
    15181 ttttccgtgg aggcggtggc aagattgccg tttttcaggt tagtcatcag cttgttgaag
    15241 aaatattgca acttacctgc ggcccactgt gcttcgtgcg cgcggcacaa aatgcggccc
    15301 aacgttgact ggataccgga aagcggcagg ttcagcgccg acatcatgcg atcgaccgac
    15361 tcaacggtcg cagcatcgcc tttgtgataa gcgattaacg tgcgtgccag cggccccact
    15421 tccatcgcgt taccgcgcca gcgcggcgct ttgatccacg agtagcgttc ctgttcattc
    15481 agctgctgaa tgttggtatc gctgcctttg acatcgccgg ggttgtacca cgggtcggta
    15541 atgccatcga atggatgacg cccgacctga tcgttgggat aacgatacca ggcgtggtcg
    15601 acaaactcct gcacctgctg cggatcaacc aaatccactg gcagcacatt gttgaagtcg
    15661 ccgttaatca ccgcgccgcc aggcatcagc agacttttct caccaaagtc gttggcaata
    15721 tccgggaacg caccgtagct gaggacacat ttatcagaaa gacccgtgcc gatttcgctc
    15781 cacggtttgt tgaactgacc gatggctaag gcgtcgggga tcatcacgtt gttaatgaag
    15841 tccgccgtgc gggtgataat cgactgcacc aggttcaggc gttccatatt gaccgccccg
    15901 accgcgccgc tttcgtcaat gttaatggcg caaggcatcc cgccgacaat ccagtttgga
    15961 tgcgggtttt taccgccaaa gaccgcgtgg attttgacaa tttcacgctg gaaatcgaga
    16021 gcttcgagat agtgggcaaa gcccatcagg ttagcttctg gcggcagttt gtactgcggg
    16081 tgcccccagt agccattgcg gaagatcccc aactgcccgc cttcaacaaa ttttttcagg
    16141 cggttttgta cgtcgaagaa atagccaggg gatgatttcg gccaagagga gagactttgc
    16201 gccagttcgg aggttttccg cgggtcggct ttcagcgcat ctaacacatc gatccagtcc
    16261 atcccggcaa gctgatagaa gtgcaccaga tgatcgtggc accagagcgt tgccagcata
    16321 atgttgcgga tgatattggc gttgtccggc actttaatac cgatagcatc ttcgatggcg
    16381 taaaccgaag ccagggcgtg tacgccagta cagacgccgc agatacgttc aacgaacgcc
    16441 cacgcatcgc gcgggtcgcg cccttgtagg atgatctcca gcccgcgaaa catggtgccg
    16501 caggagacgg cattggtgat cacattctga tcgttaatat tcacttcgca gcgcatgtgg
    16561 ccttcgatgc gcgtaatcgg gtcgaccacc aggcggcgtc cggcattatt gatggtgtat
    16621 ccctgagttt cgtactgagt gctcatgcct gtttatcctc attgcctggc tgatgttcgg
    16681 tttctgtagg ttgctggtta tgacgtctgc gctggtcaac ggcgctggcg actgcgtgca
    16741 caccaacagc cgctgccacc acaccaagcg cggttaaacc gacggtatcg gcggtggaat
    16801 gagtacccat ttgcggaata tcgaccacgc ggctgtagaa cgaaccgcaa tcccagaaac
    16861 cattttccgc acagcccagg cagccgtgac cagactggat tgggaaagaa acgccatcat
    16921 tccagcgtgt ggaggaacag gcgttatagg tggtaggccc tttgcagccc attttgtaca
    16981 ggcagtaacc tttgcgggca gcgtcatcat cccaactctg gacgaactct ccggcgtcga
    17041 agtgggcgcg gcgatagcat ttatcgtgga ttcgctgacc atagaacatc agcggacggc
    17101 ccattctgtc gacatctggc aagcgatcaa aggtcaccat gtaagtaatg atggcgctca
    17161 tcacatccgg gatcggcggg cagccaggta ctttgataat gggtttgtcg gtgatgactt
    17221 tgtcgatagg cgttgcctgc gtcggattgg gccacgcggc ctgcacgcag ccccaggacg
    17281 cgcaggttcc ccaggcgata atcgcgctgg ctccggcagc ggcacgtttg agtttctcaa
    17341 taaacggtcg accgctgctg atacagaaca tcccctgctc gcccagcggc ggattacctt
    17401 ctactgcgag gatatatttg ccattgtatt gcgtgatgat gtcttcgaag acttcttccg
    17461 cctgggttcc ggcggcagcc atcaaagtat cgtcataatc gagggaaatc agggaaagga
    17521 tgacgtcctt cgccagtggg tgagcggagc ggataaaaga ttcggtacag caggtgcatt
    17581 ccagaccgtg gatccatacc accggaatgc ggggtttgtt ctccagcgcc caggcaatct
    17641 ttggtgccat tcccgcgcct aatcccagcg acgtggcagc cagactacaa tatttgagaa
    17701 agctgcgccg ggtaacgccc tgacgccgca tggcctggta aaatgtttcc tcgttattca
    17761 tatcgcacgt ctcttctcct tgcgacaccg gcaggacacc gggcgcttat ggtcggcaag
    17821 cgacgtccct gtagcaaggc aaaccatcgc ttcacgtgag atgctgaaaa cgaaagctca
    17881 tccttctgca ctggcgcacg tcgccagaaa gtattgttaa taaagcgtag tgaaact
//
LOCUS       DAZOYL010000060        17911 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZOYL010000060 DAZOYL010000000
VERSION     DAZOYL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.060"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..788
                     /locus_tag="ACHUOY_001463"
     CDS             <1..788
                     /locus_tag="ACHUOY_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0682277.1"
                     /translation="KIRANQLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAG
                     SMRSVLSDMFREAIVEGRISQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWF
                     PLAMDLALVTGQRREDITNMRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGT
                     VVERCRLVSRGDYLISAGIRKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEI
                     RSLAGRLYKETCGEEFAQRLLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1181..1522)
                     /locus_tag="ACHUOY_001464"
     CDS             complement(1181..1522)
                     /locus_tag="ACHUOY_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR0682278.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1535..2407)
                     /gene="copD"
                     /locus_tag="ACHUOY_001465"
     CDS             complement(1535..2407)
                     /gene="copD"
                     /locus_tag="ACHUOY_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR0682279.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2411..2785)
                     /gene="yobA"
                     /locus_tag="ACHUOY_001466"
     CDS             complement(2411..2785)
                     /gene="yobA"
                     /locus_tag="ACHUOY_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR0682280.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2924..3154
                     /gene="holE"
                     /locus_tag="ACHUOY_001467"
     CDS             2924..3154
                     /gene="holE"
                     /locus_tag="ACHUOY_001467"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR0682281.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3256..3912
                     /gene="yobB"
                     /locus_tag="ACHUOY_001468"
     CDS             3256..3912
                     /gene="yobB"
                     /locus_tag="ACHUOY_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR0682282.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            3936..4598
                     /gene="exoX"
                     /locus_tag="ACHUOY_001469"
     CDS             3936..4598
                     /gene="exoX"
                     /locus_tag="ACHUOY_001469"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR0682283.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4595..6655)
                     /gene="ptrB"
                     /locus_tag="ACHUOY_001470"
     CDS             complement(4595..6655)
                     /gene="ptrB"
                     /locus_tag="ACHUOY_001470"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR0682284.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(6864..7523)
                     /locus_tag="ACHUOY_001471"
     CDS             complement(6864..7523)
                     /locus_tag="ACHUOY_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR0682285.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(7850..8206)
                     /gene="yebF"
                     /locus_tag="ACHUOY_001472"
     CDS             complement(7850..8206)
                     /gene="yebF"
                     /locus_tag="ACHUOY_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR0682286.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8273..8563)
                     /gene="yebG"
                     /locus_tag="ACHUOY_001473"
     CDS             complement(8273..8563)
                     /gene="yebG"
                     /locus_tag="ACHUOY_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR0682287.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            8697..9875
                     /gene="purT"
                     /locus_tag="ACHUOY_001474"
     CDS             8697..9875
                     /gene="purT"
                     /locus_tag="ACHUOY_001474"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR0682288.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(9931..10572)
                     /gene="kdgA"
                     /locus_tag="ACHUOY_001475"
                     /gene_synonym="eda"
     CDS             complement(9931..10572)
                     /gene="kdgA"
                     /locus_tag="ACHUOY_001475"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR0682289.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10609..12420)
                     /gene="edd"
                     /locus_tag="ACHUOY_001476"
     CDS             complement(10609..12420)
                     /gene="edd"
                     /locus_tag="ACHUOY_001476"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR0682290.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12655..14130)
                     /gene="zwf"
                     /locus_tag="ACHUOY_001477"
     CDS             complement(12655..14130)
                     /gene="zwf"
                     /locus_tag="ACHUOY_001477"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR0682291.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14468..15337
                     /gene="hexR"
                     /locus_tag="ACHUOY_001478"
                     /gene_synonym="yebK"
     CDS             14468..15337
                     /gene="hexR"
                     /locus_tag="ACHUOY_001478"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR0682292.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15465..16907
                     /gene="pyk"
                     /locus_tag="ACHUOY_001479"
     CDS             15465..16907
                     /gene="pyk"
                     /locus_tag="ACHUOY_001479"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR0682293.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(16967..17809)
                     /locus_tag="ACHUOY_001480"
     CDS             complement(16967..17809)
                     /locus_tag="ACHUOY_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR0682294.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4550 a         4521 c         4334 g         4506 t
ORIGIN      
        1 ataaaattcg ggcaaatcaa ctggagacaa taaaagaaaa attgggagag attttactga
       61 cagaaataac cactcgccat attgccgagt ttcttgattt gtggattgaa ggagggaaaa
      121 acacaatggc aggatcaatg cgttctgtgt tgtctgatat gtttcgcgag gccattgttg
      181 aaggacgtat atctcaaaat ccagtcacgc caacaagagc accgaaaata gtagttacaa
      241 gagaacgact gaaactaaag atatacaact gcatcaggga ggcagcagat caacttccgg
      301 catggttccc attagctatg gatttagccc ttgtaacagg acaacgtcgc gaagacataa
      361 cgaatatgcg gttcagtgat atttatgatg atcgtctcca cgtcaggcaa attaagacag
      421 gaatgatgat tgctatcccc ctgtcactca gccttcctgt cgctggtcta cggcttggta
      481 cagtagttga acgatgccgc ctggtaagcc ggggagatta tctaatcagt gccgggatta
      541 gaaaaaacag ccctgacggc agcattcacc cggatggcct gacaaaaaaa tttgtcgcag
      601 ccagaaaatt cacaggtatc cagttcagtg aaaacccacc aacttttcac gagatcagaa
      661 gcctggctgg acgattgtac aaagaaacat gtggagaaga atttgctcag cgtctacttg
      721 gccacacatc ggagaagaca acaaaaatgt atcttgatga gagagaaaaa acgtacttac
      781 tgctctgatt ttaacgtaaa tggattgtta aatgtatttt ggttgtgata taaccaaaaa
      841 gaccggaata cagaaattcg agtaaatttc ggggaatttc gggggagaca tttgcaacta
      901 attgatttca aatgcaatta aaaaaagacc gaatacgatt cctgtattcg gtccagggaa
      961 atggctcttg ggagagagcc gtgcgctaaa agttggcatt aatgcaggct tagttgcctt
     1021 gccctttaag aatagatgac gacgccaggt tttccagttt gcgtgcaaaa tggtcaataa
     1081 aaagcgcggt ggtcatcagc ttaaatgtta aaaaccgccc gttctggtga aagaactgag
     1141 gcggtttttt tattggaaat caaaaggcta ttttaggtaa ttaacagagt ttttcagctc
     1201 gttctataaa cggtgccaga ctcatttttt cgccgggatt gttaggatca tcaatctgaa
     1261 tcaccgaaat gggttgggca ttggtcttcc cactggcaac ttccttttgt gcgatatcgt
     1321 ttaaaggata ctgcacgagg gtactcggat taataacata caaagcatta cccggtcggc
     1381 aagtcagcat cacctcttcg cgattaaacg cccatttgtc tttacccact tcaaaacgac
     1441 tgacggtaat cacctgcggt gcagccagcg ccgctgcaga actggtgagt aacagaaacg
     1501 ccagaatact ttttttcatc attttcgtaa tccatcaaaa aggttcgaga gtcgcaagca
     1561 ggctgatgat caccagcact accgcaccta tcgcccattc gagtttagtc atccacacaa
     1621 accagggtgc cgcacgatct tcctcctgcc tcatgcgcgg tacaagaaca taacgattag
     1681 ccagcgcaat gaccaccata atcatcacca ggatggcttt gagcaataag agttggcccc
     1741 agtaagtcgt aagcgtgggg ggaaatccgg taatcaacaa tgcattaagc acgccgcttg
     1801 ccagtacgcc gatcaccgca aaatgcccgc accatgaaaa acacatcagc gcctgaatag
     1861 cctggtgtcg ccaacggcct ttgatgagtt gcatacacca gagcaccggc agtaatcccc
     1921 caaaccacgc cgccgcacaa atcaggtgaa tcgcgtgatt agtctgatgg attttcgccg
     1981 ttaccccttc attcagtgtc gcatgcccca ttcccgccag cagaataaat tgcgcggtag
     2041 tgagcataaa cagcaaacgt ggcatattcc gcggttgcat aagggcgacg atcaacgtta
     2101 cgagggcgag aacaatttgc cataaccaga cgccaccaaa ctgcgtttgc aaaacagcct
     2161 gccaaatgtt tggcgaaaat acatctgtcc atcccgttcc catcaacccg ccctgaacgg
     2221 cgagcattgc agtggcactg atcaaactcc agacggcagc gtgctgttgt aaacgcagaa
     2281 aacgcttcgc cagcaagcga cgaatcgtca agggggccag ccaggcaccg tacattgcga
     2341 agccaaaaac cagcatcagc gaggtgaaat ggataaatcg cagcgcgatc caggtaaacg
     2401 ccagcatgat ttatttcacg ctaaaggtgt agtgtccttt cgttttatgc ccgtccaccg
     2461 aaacaacatg ccagtcaacg gtgtaggttc cgggtttcag cgaatcggcc agtggaacaa
     2521 tcagttgctt ttggtcctgc tcatttcgct tcgccggtaa tgttttaata ttttcgtttt
     2581 ttggccccgt gatttttgca ccactgaatc ctgtttccac accttccgag aagtttaaag
     2641 tgattgcctg cggtgcagct gtcacttgcg cgtttgccgc aggatactga tgcgttaaat
     2701 gcgcatgtgc ccagacgaaa ggtgtcacca acgaagtggt caggattgcc agcgcgtagc
     2761 gaagggagcg tgcagttgaa gccatattat ctattccttt ttgtaataac ttttttacag
     2821 agcataacct tgtctaatgt ctgagtcgag gatcatcaat tccggcttgc catcctggct
     2881 cactcttagt aacttttgcc cgcgaatgat gaggagatta agaatgctga agaatctggc
     2941 taaactggat caaacagaaa tggataaagt gaatgtcgat ttggcggcgg ccggggtggc
     3001 atttaaagaa cgctacaata tgccggtgat cgctgaagcg gttgaacgtg aacagcctga
     3061 acatttgcgc agctggtttc gcgagcggct tattgcccac cgtttggctt cggtcaatct
     3121 gtcacgttta ccttacgagc ccaaacttaa ataaaactta tacagggtta cactttctta
     3181 cataacgcct gctaaattat gagtattttc taaaccgcac ccataatttg cagtcatttt
     3241 gaaaaggaag tcattatgtc gttctggaaa gttgcagcag cgcaatatga gcctcgtaaa
     3301 acctcactca ccgaacaagt agctcatcat ctggagtttg tgagggccgc cgccaggcaa
     3361 caatgtcagc tgttagtttt tccatcgctt tctttactgg ggtgtgatta ttcgcgacgt
     3421 gcccttcctg ccccacccga tctctcactg ttagacccgc tttgttatgc cgcaacgacc
     3481 tggcgaatga ccatcattgc cggccttccc gttgaatata acgatcgctt tattcgtgga
     3541 attgcggtgt tcgcaccgtg gcgaaaaacg cctgggatct accatcagag tcatggtgca
     3601 tgtctgggcc gtcgttccag aaccatcacg gtggtcgatg aacaaccgga aggtatggat
     3661 atggacccga cctgttcact gtttacgaca gggcaatgtc tgggagaacc cgacctgctg
     3721 gcgtcggccc gtcgcttaca atttttttca catcagtact cgattgctgt gctgatggca
     3781 aatgcccgtg gtaacagtgc actatgggat gaatatggtc gtctcatcgt tcgcgccgat
     3841 cgtggttcat tattgttagt cggtcagcgt tcatcacagg gttggcaagg cgatatcatt
     3901 ccattacgct aggctttttc ggcctggagt atgccatgtt gcgcattatc gatacagaaa
     3961 cctgcggttt gcagggaggg atcgttgaga ttgcctctgt tgatgtcatt gacggaaaaa
     4021 tcgtcaaccc catgagccac ctggtgcgcc ccgatcgtcc tattagtcca caagcgatgg
     4081 cgattcatcg catcaccgaa gccatggtcg ccgataaacc gtggattgaa gatgtgatcc
     4141 cacactatta cggcagtgaa tggtatgtcg cgcacaacgc cagctttgac cgccgcgtac
     4201 tgcctgagat gcccggtgag tggatttgca ccatgaaact ggcccgtcgc ttgtggccag
     4261 gaatcaagta cagcaatatg gcgttatata aaacacggaa gctcaatgta cagacgccgc
     4321 cgggcctgca tcatcaccgc gcgttgtatg actgttatat caccgccgcg ttgcttatcg
     4381 atattatgaa cacctccggc tggacggcag aacagatggc cgatatcacc ggacgtccgt
     4441 cgttgatgac gaccttcacc tttggcaaat accgtggcaa agcggtttcc gacgttgccg
     4501 aacgcgatcc gggctatctg cgctggttat ttaataacct ggacagcatg agcccggagc
     4561 tgcgtttaac actgaaacat tatctggaaa atacttaatc cgcaggcgta gcgggtaatg
     4621 ttccctgcgc cagcgcgacc agaaaagcat attccatcgc tacgccttcg tacgatttaa
     4681 agcggccaga tttaccgcca tggcctgagt ccatgtcggt acagagcaat aaaagatggt
     4741 tatcggtttt cagctcgcgc aatttagcga cccattttgc cggttcccaa tattgcacct
     4801 gagaatcgtg taaaccggtc gttaccagta aatgcggata agcctgtgcg gtgacgttgt
     4861 catacgggct gtagcttttc atgtactcgt aatattgcgg atcctgcggg ttaccccact
     4921 cttcaaactc accagtggta agaggaattg attcatcaag catcgttgtt acaacatcaa
     4981 caaacggtac ctgggcgata acgccgtgga ataattccgg gcgttgatta attgcaacgc
     5041 ccatcaacat gccccccgca ctcccgccca tcgcataaca aagcgaagga gagccatagc
     5101 ccagttttaa caatgcatcg caggcatcaa gataatcatt aaacgtattt ttcttcttca
     5161 gaaattttcc gtcttcgtac cattgttgcc ccagctcacc accgccgcga acatggacaa
     5221 tggcgtagac aaagccacga tctaacaaac tcaagcggct aaaactgaaa tcggcatcaa
     5281 tacttgcgcc gtaagaacca tagccataca ccagcaacgg gttgtgtcct ttgcgaaaat
     5341 gtttgcgatg gtagaccaac gaaaccggaa cttcgacgcc atcacgggcg actatccaca
     5401 ggtgttcact gcggtaattc gccgcatcaa aaccaggaac ttccgtttgt tttaatacac
     5461 gacgctcatc ggtatccata tccagttcaa acaaagtgtc tggtgtggtc atggaagaat
     5521 aaccataacg caatcgcgcg gtttcaggtt ctggattgta ggcaatccag gtcacatagg
     5581 ctggatcatc aaaggcaata ccaatgactt cccgggtctt gcggttaatt tggcgcaaac
     5641 tggttaaccc gcgctgacgc tcttcaacca ccagccagtc ggtaaacagc gtaaaccctt
     5701 ccagcatgat gttttcgcat ggcggaatta actcttccca ctgttgctca tcacgcatac
     5761 gggtacggta taagccaaag tttttgccgt ggcggttgga acgcagataa aaacgatgct
     5821 ggtagtgatc aaggctgtat tcgtgatctt tgcggcgcgg cagaaaaaca aacggctcgg
     5881 catcggccat ttccgcgtcc agcaggcgaa cttcactggt ggtggcgctg gccaaatgaa
     5941 tgactacata gtgcttcgag gtcgttttat gcaggctgac gtaataggta tcgtcttttt
     6001 cttcgtagat cagtttatct tgcgatgctg gcgtaccgat ggcgtgacgc cagacctgat
     6061 aaggcagcag cgtcaccgga tgcttgcgaa catagtagaa agtccaggag tcatttgccc
     6121 agacaaagct gggttcaacg ttatccagca gttccgggta ccagttacca gtttccagat
     6181 tacgaaaacg aatgccgtac tggcgtcggg aaagaaaatc ttctgccagc gccataatgg
     6241 tgttatcggg cgtaatcgcc attccgccca tcgaataaaa ctcactatga gctgcgcgct
     6301 tattggcatc gagcaatatt tcccactcat cccactcttc actgaatgcc gattgacgct
     6361 ggtagatagc atattcacag cctggttcat aaatatgccg atagcggtag ccatttttga
     6421 tgtagggcgc agaaacttct cgttgcggaa tgcggtcgat gatttccttt aagatgcgat
     6481 cctgcaaggc ttgttgtgag gccatcaccc gatgaccgta actattttct tgttgcaggt
     6541 agtccaggac ttctggctga gaacgcgtat catcccgcag ccagtagtaa ttatcgatgc
     6601 gcgtatcgcc atgaagcgtc atggcgtggg gaatgcgggc ggcttttggt agcatgttat
     6661 tgttctttct ggttgaaaca tcttataagg gtggcaaaac tcaccgggga tgcaagcgaa
     6721 acaggggagt cattgcttag atgatgacag gtaatggcac ggatatcgaa tgttatgcag
     6781 acagagaaac tcagcctgtt caaatggctg tgcggttctg gatagcccga aatagtcaac
     6841 ttcaggctat ccagagagcg gaattattcc gccaaagtgc gtttttgctg ttcgagatcg
     6901 cgttcaatgc cgtcacgaac atcctggggg attttcagcg cgtcacccag tgcattcagg
     6961 taactgcgtt ccataaaatg gtcaatatca atagccgcgc aactcaggaa atagatttcc
     7021 agcgcctctt cttcattgcg gaccccggta gccaggcgtt gtggatccag cggttgttcg
     7081 attgcctgct caatgagtac acgcccctgc tcttccacgc cggcttcacg caattgctgg
     7141 tcgattgccg cacgttcttt ggcatcaata tgaccatcac ttttagcggc aaagaccagc
     7201 gcaaggatca aacgttctgt acgctcatcc agcggcgtac tttgcgcgcc aaactgcggt
     7261 tcgtcctgat gcgccgcgcg aattttatct ttgtatttat tccacagcac cgtacccgct
     7321 accgctccgc cgccaaccag taacgcgttg gtgccatatt tcgtaagaag tttacgtgct
     7381 gatttatttg cgaccagcag cccagccagt ccgcctaatg cgcctggcac taacagtttg
     7441 cccaatccct gatccgcaga cgaggaggta gaagaactgc tttgcccaag cagggattgc
     7501 agttgattta accagttagc catatttgct cctcaataac catttttagt gcctgtagca
     7561 tagcggatga ggatgtcaga aaatgtatat cgagacaaaa gatgcgcaaa taagcggggc
     7621 ggtaggccgt tattcaaaga aattcgcgcc aggtaaggta tttactgtat atcaggtgat
     7681 aagggtattt taggtgaaag tagtttgtcg ttttgagtgg cattgtttga tgctgattgc
     7741 ccgatgcgac gctgttgcgt cttctcaggc ctacgattgt ggtgcgttct ggggcggaca
     7801 aggcactcgc gccgcatcca ccaacggtca atcgaattat cagaagccat taacgccgct
     7861 gatattccgc cattcccgct ttgcagtcca cgctgacctg gtaatgaata tcggcacttt
     7921 taccacgcac ggttagcggt actgaccatt tatcatcttt accctgaatg tcctgcaaac
     7981 tgacccaagc cacgggatcg gcctgaccga caattttttg atcatctgcc caacgcgcca
     8041 cgcgattttg ttgataatca cgttttacgc tcgcggcaat tccggcagca tccagacctt
     8101 cacactttgg gaaagtgacc gacttgctgg tttcattatt ggcagcgaaa actgatgcgc
     8161 aggcagaaac caacaacagc cctaaaaacg cccctctttt tttcatgttt ttctccatag
     8221 cacaatgatt caggagaaag catggtacaa attgtcagga gcgcaagttg cttcaggccg
     8281 cgtgtgaggc atcttcctct tcggattcag caccgacgac ctgtggggac ggtaatttgc
     8341 cggttttcag aatggtgctc agcacatctt tttgttctgc cagccatagc gaaagggctt
     8401 cacgttgctc gtcttccatt tgcactggag aatttgtcag ccaggtgtca agcagatccg
     8461 ccgtatcaag cattttgtca taagcatcgg cttccttttt gctggtaaac gacattttct
     8521 cttcgccctc acgaatgact acgtatttaa cttcaaccgc catttgcagc ctctcataat
     8581 aactgtgatt ttatacagta tatttctttt cggttgagaa atcaacatca gcaataaaga
     8641 cacacgcaaa cgttttcgtt tatactgcgc gcggaattaa tcaggggata ttcgttatga
     8701 cgttattagg cactgcgctg cgtccggcag caactcgcgt gatgttatta ggctccggtg
     8761 aactgggtaa agaagtggca atcgagtgtc agcgtctcgg cgtagaggtg attgccgtcg
     8821 atcgctatgc cgacgcacca gccatgcatg tcgcgcatcg ctcccatgtc attaatatgc
     8881 ttgatggtga tgcattacgc cgtgtggttg aactggaaaa accacattat atcgtgccgg
     8941 agatcgaagc tattgccacc gatatgctga tccaacttga agaggaagga ctgaatgttg
     9001 tcccctgcgc tcgcgcaacg aaattaacga tgaatcgcga gggtatccgt cgcctggcgg
     9061 cagaagagct gcagctgccc acttccactt atcgttttgc cgatagcgaa agccttttcc
     9121 gcgaggcggt tgctgacatt ggctatccct gcattgtaaa accggtgatg agctcttccg
     9181 gcaaggggca gacgtttatt cgttctgcag agcaacttgc tcaggcatgg aagtacgctc
     9241 agcaaggcgg tcgcgccgga gcaggccgcg taattgttga aggcgtcgtt aagtttgact
     9301 tcgaaattac cttgctaacc gtcagcgcgg tggatggcgt ccatttctgt gcaccggtag
     9361 gtcatcgcca ggaagatggc gactaccgtg aatcctggca accacagcaa atgagcccgc
     9421 ttgcccttga acgtgcgcag gagattgccc gtaaagtggt gctggcactg ggcggttatg
     9481 gtctgtttgg tgtcgagctg tttgtctgtg gtgatgaggt gattttcagt gaggtctccc
     9541 ctcgtccaca tgataccggg atggtgacgt taatttctca agatctttca gagtttgccc
     9601 tgcatgtacg tgccttcctc ggacttccgg ttggcgggat ccgtcagtat ggtcctgcag
     9661 cttctgccgt tattctgcca caactgacca gtcagaatgt cacgtttgat aatgtgcaga
     9721 atgccgtagg cgcagatttg cagattcgtt tatttggtaa gccggaaatt gatggcagcc
     9781 gtcgtctggg tgttgcgctg gcgaccgctg agagtgttgt tgacgccatt gaacgcgcga
     9841 agcacgccgc cggacaggta aaagtacagg gttaaacccg gcaaaaaaac gctacaaaaa
     9901 tgcccgatcg tagatcgggc gttttgactt ttacagctta gcgccttcta cagcttcacg
     9961 cgccagctta gtaatgcggt cgtaatcgcc cgcttccagc gcatctgccg gaaccagcca
    10021 ggaaccaccg atgcacagca cgcttttcag cgccaggtag tcacggtagt tagccggaga
    10081 aataccaccc gtcgggcaga aacggacctg ggagaacgga cccgcgatcg cctgcagggc
    10141 tttcacgccg ccgttagctt cagccgggaa gaatttgaac tctttcaaac cgtagtccat
    10201 acccagcatc agttcggaaa cagtgctgat ccccggaatt agaggaatag tcccttcggt
    10261 agcagctttc agcagcggct cggtcagacc cgggctaatt gcgaactgtg cacccgcttc
    10321 agtgacttct gccagctgct gtggattcag caccgtaccg gcacccacaa tcgcttcagg
    10381 cacttctttg gcgatagcac ggatagcgtc aactgcacac tcggtacgca gagtcacttc
    10441 cagaacgcgc accccaccag caaccaacgc ttttgccatc ggcactgcgt gttccagttt
    10501 ttttaccacg ataaccggta caaccgggcc ggtggtcagg attgattctg cacttgtttt
    10561 ccagtttttc atcagagttt tctctcgcct gattacaaat ttgtcgtctt aaaaagtgat
    10621 acaggttgcg ccctgttcgg caccggacag tttttcacgc aaggcgctga ataattcacg
    10681 tcctgtcccc acgcgtgacg cgctcaggtc aggaatgtgc ggttcgcgag cagccagttc
    10741 cgcttcgtct accagcagcg tcagttcgcc tgtctgtcca ttcacacgaa tgatgtcccc
    10801 gtcgcgcact tttgccagca gcccgccatc gtaggcttct ggtgttacgt ggatagctga
    10861 cggcacttta cctgaagcgc cggagagtcg tccatcggta actaacgcaa ttttgaaaca
    10921 ccggtccaat aatacaccaa gtggcggcat gagtttatgt aattctggca ttccgttcgc
    10981 ttttggcccc tgatgacgga caacaacgac acaatcgcgg tccagcaaac ccgcttcaaa
    11041 ggccggcata acgtcatgct ggctttcaaa aacaaccgct ggcgcttcaa tcacctggtt
    11101 ctcaaccggc acggcagagg ttttcataac cgcacggccc aggttaccgc ttaacacttt
    11161 tgtcccacca tgatgagaga aaggttgttc gaaggaagcg atcacattgc tgtcgagtga
    11221 tttttccgcc ccttcccgcc agtcctgttc accattattc agccatggtt caagggtata
    11281 acgagacaga ccaaagcctg ccaccgtatt gacatcttca tgcagcaggc ctgctttgag
    11341 cagttcacgc accagaaccg gtacgccacc tgccgcctgg aagtggttaa tatcggccgg
    11401 accgttcggg tagagacgtg ccatcagcgg tacaacatca gaaaggtcag agaagtcatc
    11461 ccagttaatc tgaataccgg ccgcgcgcgc catcgccacc aggtgcatgg tgtggttagt
    11521 ggaaccaccg gtcgccagca gtgcaacgat accgttcacc accactttct catcgatcat
    11581 cttaccgatc ggcatccatt cattaccatt accggtcatg cgtgtaacct gacgcgcagc
    11641 ggcagcggtc aaagcatcgc gcagcggaga atccggatga acaaaagaag agcctggcaa
    11701 ctgcatcccc ataaattcca ccaccatctg gttggtgttg gcagtaccgt agaaagtaca
    11761 tgttcccggc gcatggtaag acgcggcttc tgactccagt aaggccatgc ggtccacttt
    11821 accttcggca taaagctggc gaatacgcac tttttcttta tttggcaaac cgcttgccat
    11881 cggtccagac ggcacaaaca ccgcaggcaa atgaccaagc gacagggctg ccatcgtcag
    11941 acccgggaca atcttgtcgc acacaccgag gaacagagca ccatcaaaca tgttatggga
    12001 cagccccacc gccgcagaca tcgctatcac ttcgcggctt agcagcgaca attccattcc
    12061 atcctgcccc tgggtgacac catcacacat cgccggaaca ccgcccgcaa cctgaccaac
    12121 cgcattcgct tcatgcaggg ctttacgaat gatttctgga tagtgttcat aaggctggtg
    12181 cgcggagagc atgtcgttat aggaggtgat gatggcgata ttgttacgca acatgctttt
    12241 caaagaggct ttgtcttctg gctggcaggc agcgaaaccg tgtgccaggt taccgcatgc
    12301 caactgcgaa cgatgaacgg tcgacgtttt cgcttgttct atccgggcga gataagcaga
    12361 gcgagtctcg cgcgaacgtt caatgattcg atttgttacg cataacaatt gtggattcat
    12421 aaaggctcct gaaattgagt tgtcagagca ggatgattca caacgcgttt cattcagagg
    12481 atttatgagt gaaacgcctg taaccggagc tcatagggca aaaacgtttc agtcagtgta
    12541 ataaaaaaag cctcgtgggt gaatccgcac gaggcctgaa agtgtaaaaa ttgttctaca
    12601 atctgtgcaa gatcatgtta ccggtaaaat aaccataaag gataagcgca gatattactc
    12661 aaactcattc caggaacgac catcacgggt aatcatcgcc accgaggcaa cgggtcccca
    12721 ggttccggcc tgatacggtt tcggcgcatc attgtccatc gcccacgcct cagtaatgga
    12781 gtctacccat ttccaggctt cttccacttc atcacgacgt acaaacagtg cctgaatacc
    12841 acgcatggtt tccagcagca gacgttcata ggcatccgcc agatgcgtct gattaaaggt
    12901 ttctgaatag ctcagatcca gcttggtgat ttgcaggtta tgtttgtggt caaggccagg
    12961 aactttattc agtacctgga tatccacgcc ttcatcaggt tgcagacgga tagtcagttt
    13021 attctgcggc agatcctgcc acgactcttt aaacagattc agttcaggtg ttttgaaata
    13081 gaccacgact tcagaacatt tggtcggcag acgtttacca gtacgcaggt agaatggcac
    13141 accggcccag cgccagttat caatgtcgac gcggatcgcc acgaaggttt ctgtattgct
    13201 gctcttgttc gcgccctctt cttccagata tcccggcact tttttgccct gggcgaagcc
    13261 cgcagtatat tgcccgcgta cggttttttc gcgtacgttg gagcggtcga tgcggcgcag
    13321 ggacttcagt actttcactt tttcatcgcg gatgctgtct gcgctcaggt cagatggcgg
    13381 agacatcgca atcatgcaga gaatttgtag caggtggttc tgaatcatgt cacgcatctg
    13441 accggcttta tcaaaatagc cccagcgccc ttcgatcccc acttcttctg ccacggtaat
    13501 ctcaacatga tcaatggtgc gattgtccca gttattcaca aacagggagt tagcaaaacg
    13561 cagcgccaac aggttcagca ccgtttcttt accaagatag tggtcgatac ggtaaacctg
    13621 gcactcctcg aagtattcgc caacctgatc attgatttcc tgcgaggtcg ccagcgacgt
    13681 ccccagcggt ttctccatga ctacgcgtgc cggtttagca ttcagttttg cctcgccaag
    13741 ccctttgcaa attgcgccaa aagtgctggg cggcatggca aagtagttaa tggtgatacg
    13801 atttttttga tccagcatcg cgccgagacg gttgaatgca gcagtgtcat tgacatcgag
    13861 attacaaaaa tccagacgtg cactcagggt gtcccataaa ccttcatcaa tggtttcttt
    13921 catgaaagtt tcgagcgcct cgcggacaac tttggtatat gccgctttat cccagtcagc
    13981 acgccctacg ccgataatcc gggtgtccgg gttgagctga ccggcttttt ccagttgata
    14041 cagggaaggc agcaatttac gacgcgcaag gtcgcctttc gcgccgaaaa tgaccaggtc
    14101 acaggcctgg gctgtttgcg ttaccgccat gtcattctcc ttaagttaac taacccggta
    14161 cttaagccag ggtatacttg taattttctt acggtgcact gtactgcttt tacgagcttg
    14221 cgaaaactgt aaacgcttat ccacccgtgc gattacggga aaagcgcgca aagtgcggca
    14281 aaactgataa aaaaatcatc gttttccatt cggtaaaaat ctgacactga tcatgttatg
    14341 aaaaaaaata acaacttttt tatctgcttt tgtcattaac acggcacaca ggcgtaatat
    14401 ctgacaaaac tgcatttcga tttctttcag tgcggaaatc gtcattaccc gtgagtctct
    14461 ttacatcatg aatatgctgg aaaaaatcca gtctcagctg gaacatttga gcaaatcaga
    14521 gcgcaaagtt gccgaggtca ttctggcttc gcccgataac gcgatccatt cgagtattgc
    14581 tgctatggca ctggaagcca atgttagcga accgacggtg aatcgtttct gtcgcagcat
    14641 ggacacgcgc ggttttcctg attttaaact tcatctggca cagagtctgg cgaatggcac
    14701 tccctatgtt aatcgcaatg tcaatgaaga tgacagcgtt gaatcataca cagggaaaat
    14761 atttgagtcc gcaatggcaa cgcttgatca tgtccgtcat tcactggata aatctgccat
    14821 caaccgcgcc gtcgacttgc tcactcaggc aaaaaaaatc gcctttttcg gattaggctc
    14881 ttcagccgcc gttgcccacg atgcgatgaa taagttcttt cgttttaatg ttccggtggt
    14941 gtactccgat gatatcgtgc tgcaacgcat gagttgtatg aattgtagcg acggagacgt
    15001 ggtggtgctg atttctcaca ctggaagaac aaaaaatctg gtcgagctgg cgcagctggc
    15061 tcgcgaaaac gacgcaatgg tgattgccct cacctctgcg ggtaccccgc tcgcccggga
    15121 agcaacgctg gcaattaccc tcgacgtacc ggaagatact gacatttata tgcccatggt
    15181 ttctcgactt gcacagctga ccgtgataga tgtgctggcg acaggattta ctttgcgacg
    15241 cggtgcaaaa ttcagagata acttgaagcg ggtcaaggaa gcgctgaagg aatcgcgttt
    15301 tgataagcag ttacttaatt taagtgacga tcgctaaaaa cgactgtcac tgtcctaatc
    15361 ttatacgaca tccgaatgag attaatttat cgccatcgcg gcgttatttc attcgcattt
    15421 catgttcaag caacacctgg ttgtttcagt caacggagta ttacatgtcc agaaggcttc
    15481 gcagaacaaa aatcgttacc acgttaggcc cagcaacaga tcgcgataat aatcttgaaa
    15541 aagttatcgc ggcgggtgcc aacgttgtac gtatgaactt ttctcacggc tcgcctgaag
    15601 atcacaaaat gcgcgcggat aaagttcgtg agattgccgc aaaactgggg cgtcatgtgg
    15661 ctattctggg tgacctccag gggcccaaaa tccgtgtatc cacctttaaa gaaggcaaag
    15721 ttttcctcaa tattggggat aaattcctgc tcgacgccaa cctgggtaaa ggtgaaggcg
    15781 acaaagaaaa agtcggtatc gactacaaag gcctgcctgc tgacgtcgtg cctggtgaca
    15841 tcctgctgct ggacgatggt cgcgtccagt taaaagtact ggaagttcag ggcatgaaag
    15901 tgttcaccga agtcaccgtc ggtggtcccc tctccaacaa taaaggtatc aacaaacttg
    15961 gcggcggttt gtcggctgaa gcgctgaccg aaaaagacaa agcagacatt aagactgcgg
    16021 cgttgattgg cgtagattac ctggctgtct ccttcccacg ctgtggcgaa gatctgaact
    16081 atgcccgtcg cctggcacgc gatgcaggat gtgatgcgaa aattgttgcc aaggttgaac
    16141 gtgcggaagc cgtttgcagc caggatgcaa tggatgacat catcctcgcc tctgacgtgg
    16201 taatggttgc acgtggcgac ctcggtgtgg aaattggcga cccggaactg gtcggcattc
    16261 agaaagcgtt gatccgtcgt gcgcgtcagc taaaccgagc ggtaatcacg gcgacccaga
    16321 tgatggagtc aatgattact aacccgatgc cgacgcgtgc agaagtcatg gacgtagcaa
    16381 acgccgttct ggatggtact gacgctgtga tgctgtctgc agaaactgcc gcagggcagt
    16441 atccgtcaga aactgttgca gccatggcgc gcgtttgcct gggtgcggaa aaaatcccga
    16501 gcatcaacgt ttctaaacac cgtctggacg ttcagttcga caatgtggaa gaagctattg
    16561 ccatgtcagc aatgtacgca gctaaccacc tgaaaggcgt tacggcgatt attaccatga
    16621 ccgaatcggg tcgtaccgcg ctgatgacct cccgtatcag ctctggtcta ccaattttcg
    16681 ccatgtcgcg ccatgaacgt acgctgaacc tgactgctct ctatcgtggc gttacgccgg
    16741 tgcactttga tagcgctaat gacggcgtag cagctgccag cgaagcggtt aatctgctgc
    16801 gcgataaagg ttacttgatg tctggtgacc tggtgattgt cacccagggc gacgtgatga
    16861 gtaccgtggg ttctactaat accacgcgta ttttaacggt agagtaatct cacctgcccc
    16921 ggccgctgat ggccggggta tttagcaaca cataaattag gactacttat tcaatccaat
    16981 cataatcaca gccccctgta aaatgaatac agggatagcc tgaatggaga tctaaatagc
    17041 ttgcaagacg tcgcataccc gcttcctctg caacactatg tccaataact gccatcggta
    17101 agccactatc tttggagtat tgtgtctgca cccatgtttt aattgaatcg ttaatcgtaa
    17161 tataataatc agcctgatat tccatatatt gtattggatc acaaaaacag cctgccccaa
    17221 tgccaacagt tctgactact ctggctttat ctccataaaa ttgaatgcca ggaagattat
    17281 atattttcaa atttttggca aaacgtttgg taatgttaat ggcactgtca gggtctatag
    17341 catatacaca gtacatctca tcagaagcaa caatatttgt aacatccagt tctaactgct
    17401 gagccaaagc cttagacatt ccgaacgtag gctctcgatc catgacatca tggcaacgaa
    17461 tgattaccaa attattttta tgcaaaaact cctttttttg ctcaattatt tttaaataac
    17521 tgagttcacc gcgagtataa tccattattt ttctggccca tgaaaactca agatcgtcac
    17581 cctccagatc agcataagaa taaaatgtag gctcatgtgt aataatgata ttgacacctt
    17641 caaaaacagc ctttcttaaa acatccattg aggcaatcca gcaggtccca attttattaa
    17701 taagtgtcgc ctcagcacca aaaataattt tatcaacagt tggttgcgaa acagcaacca
    17761 ttgaatttaa aaaacaatta agatccgcgg ctgttttaat atgatgcatt caacatcctt
    17821 cacaggtttc atcagcaata aaataatttc aacatttctg ggaaacgttt tgcccatgca
    17881 gactcagagt gggtatcacc tccccataaa t
//
LOCUS       DAZOYL010000061        17354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZOYL010000061 DAZOYL010000000
VERSION     DAZOYL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.061"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(17..784)
                     /gene="ygbI"
                     /locus_tag="ACHUOY_001481"
     CDS             complement(17..784)
                     /gene="ygbI"
                     /locus_tag="ACHUOY_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR0682295.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            980..1888
                     /gene="ltnD"
                     /locus_tag="ACHUOY_001482"
     CDS             980..1888
                     /gene="ltnD"
                     /locus_tag="ACHUOY_001482"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR0682296.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1885..3147
                     /gene="otnK"
                     /locus_tag="ACHUOY_001483"
     CDS             1885..3147
                     /gene="otnK"
                     /locus_tag="ACHUOY_001483"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR0682297.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3144..3782
                     /locus_tag="ACHUOY_001484"
     CDS             3144..3782
                     /locus_tag="ACHUOY_001484"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0682298.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            3787..4563
                     /locus_tag="ACHUOY_001485"
     CDS             3787..4563
                     /locus_tag="ACHUOY_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR0682299.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4652..6016
                     /locus_tag="ACHUOY_001486"
     CDS             4652..6016
                     /locus_tag="ACHUOY_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR0682300.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALCASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6110..7102)
                     /gene="rpoS"
                     /locus_tag="ACHUOY_001487"
     CDS             complement(6110..7102)
                     /gene="rpoS"
                     /locus_tag="ACHUOY_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR0682301.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7165..8259)
                     /gene="nlpD"
                     /locus_tag="ACHUOY_001488"
     CDS             complement(7165..8259)
                     /gene="nlpD"
                     /locus_tag="ACHUOY_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR0682302.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8444..9070)
                     /gene="pcm"
                     /locus_tag="ACHUOY_001489"
     CDS             complement(8444..9070)
                     /gene="pcm"
                     /locus_tag="ACHUOY_001489"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR0682303.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9064..9825)
                     /gene="surE"
                     /locus_tag="ACHUOY_001490"
     CDS             complement(9064..9825)
                     /gene="surE"
                     /locus_tag="ACHUOY_001490"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR0682304.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(9806..10855)
                     /gene="truD"
                     /locus_tag="ACHUOY_001491"
     CDS             complement(9806..10855)
                     /gene="truD"
                     /locus_tag="ACHUOY_001491"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR0682305.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(10852..11331)
                     /gene="ispF"
                     /locus_tag="ACHUOY_001492"
     CDS             complement(10852..11331)
                     /gene="ispF"
                     /locus_tag="ACHUOY_001492"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR0682306.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11331..12041)
                     /gene="ispD"
                     /locus_tag="ACHUOY_001493"
     CDS             complement(11331..12041)
                     /gene="ispD"
                     /locus_tag="ACHUOY_001493"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR0682307.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12060..12371)
                     /gene="ftsB"
                     /locus_tag="ACHUOY_001494"
     CDS             complement(12060..12371)
                     /gene="ftsB"
                     /locus_tag="ACHUOY_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR0682308.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12565..12888)
                     /locus_tag="ACHUOY_001495"
     CDS             complement(12565..12888)
                     /locus_tag="ACHUOY_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR0682309.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(12938..13543)
                     /gene="cysC"
                     /locus_tag="ACHUOY_001496"
     CDS             complement(12938..13543)
                     /gene="cysC"
                     /locus_tag="ACHUOY_001496"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR0682310.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13543..14970)
                     /gene="cysN"
                     /locus_tag="ACHUOY_001497"
     CDS             complement(13543..14970)
                     /gene="cysN"
                     /locus_tag="ACHUOY_001497"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR0682311.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(14972..15880)
                     /gene="cysD"
                     /locus_tag="ACHUOY_001498"
     CDS             complement(14972..15880)
                     /gene="cysD"
                     /locus_tag="ACHUOY_001498"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR0682312.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16132..17169
                     /gene="iap"
                     /locus_tag="ACHUOY_001499"
     CDS             16132..17169
                     /gene="iap"
                     /locus_tag="ACHUOY_001499"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR0682313.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT         4064 a         4673 c         4389 g         4228 t
ORIGIN      
        1 atgttattgc aggccattat tcatttttcg ctaccagcaa agagagatcc tgcttcgcca
       61 gcgcgcgact ggcactctcc ggcaaaccgt cgtctgtgat aatctgatca aactcgctta
      121 atggtaacgc cagccatgtc gccacctgac catatttcgt cgcatcacag accaaaactc
      181 gctggcggct ggcactggca atcgcccgtt tcaccgtgac tttatcttcc gctggcgtag
      241 aaatcccccg cacactccat gacgatgcag aaataaaagc ctgatcaatc atcaggctgc
      301 gcagcatggt cgcagcggct tccccgacac aggaacggtt ttcccgacac actgcaccgc
      361 cagtgtgaat aattgtgcaa ttactgttgt cgagcaagta gttcgcaata acgaaatcgt
      421 ttgtgaccac agtcagtgac tccatgtgaa tcagatgctg tgctatcgct aacgtggtcg
      481 ttcccgcatc cagatagata caacttcccg gctgaacaag acttgccgcc agcttgccaa
      541 tagccgcttt ttgcgtcatt gccagcgcag tttttacctg atgagaaggt tcatgcgcca
      601 cgcgtcccgg agactggacg cctccggaca ccagcacaac ggctccctgc tgctccagtt
      661 tttgtaaatc ccgacgaatg gtcatatgtg acacattcat tctgtccgtt agttcagcaa
      721 tactgacaat gcctttttca gctaccatct caaggatgat ttggcgacgc tctacgggta
      781 tcaacttttg ctccttcctt tgtcctgctg acattctacg ctatttgcct gcgaaacgtg
      841 cgcggcgcaa ctaacgctta gttcacataa aataacacac aatgttaatt tatgtgaatc
      901 agctcaccat atcgttatct tccagcgctt atattcacaa tatcaaacaa aatatcactt
      961 aaattaacaa tgagagcaga tgaaaacggg atctgagttt catgtcggta tcgttggctt
     1021 agggtcaatg ggaatgggag cagcactgtc atgtgtccgc gcaggtcttt ctacctgggg
     1081 cgcagacctg aacagcaatg cctgcgctac gttgaaagag gcaggtgctt gcggggtttc
     1141 tgataacgcc gcgacgtttg ccgaaaaact ggacgctctg ctggtgctgg tggccaatgc
     1201 ggcccaggtt aaacaggtgc tgtttggtga aacaggcgtt gcacaacatc tgaaacccgg
     1261 tacggcagta atggtttctt ccactatcgc tagtgctgat gcgcaagaaa ttgctaccgc
     1321 tctggctgga ttcgatctgg aaatgctgga tgcgccagtt tctggtggtg cagtaaaagc
     1381 cgctaacggt gaaatgactg tcatggcctc cggtagcgat attgcctttg aacgactggc
     1441 acccgtgctg gaagccgttg ccggaaaagt ttatcgcata ggtgcagaac cgggactagg
     1501 ttcgaccgta aaaattattc accagttgtt agcgggcgta catattgctg ccggagccga
     1561 agcgatggca cttgcagccc gtgcggggat cccgctggat gtgatgtatg acgtcgtgac
     1621 caatgccgcc ggaaattcct ggatgttcga aaaccggatg cgtcatgtgg tggatggcga
     1681 ttacaccccg cattcagccg tcgatatttt tgttaaggat cttggtctgg ttgccgatac
     1741 agccaaagcc ctgcacttcc cgctgccatt ggcctcaaca gcattgaata tgttcaccag
     1801 cgccagtaac gcgggttacg ggaaagaaga cgatagcgca gttatcaaga ttttctctgg
     1861 catcactcta ccgggagcga aatcatgatc aagattggcg ttatcgccga tgattttacc
     1921 ggcgcgacgg atatcgccag ttttctggtg gaaaacggtc taccaacggt acaaattaac
     1981 ggtgttccaa caggtaaaat gccggaagca atcgacgcac tggtgatcag cctgaaaacg
     2041 cgctcctgtc cggtggttga agccacacag caatcgctgg cggctctgag ctggttgcaa
     2101 cagcaaggtt gcaaacagat ctatttcaaa tactgctcta ctttcgacag tacggcgaaa
     2161 ggtaatattg gcccggttac cgatgcctta atggatgctc tcgacacgcc gtttacggtc
     2221 ttctctccgg ccctgtcggt caacggacgt acggtttatc aggggtattt gttcgtaatg
     2281 aatcaactgc tggccgaatc cgggatgcgc catcacccgg taaatcccat gaccgacagc
     2341 tatcttcccc gtctggttga agcgcaatcc acagggcgct gcggcgtcgt ttcggcacat
     2401 gttttcgaac aaggcgtgga tgccgttcgt caagagctgg ctcgcttaca gcaagagggc
     2461 taccgctacg cggtgcttga tgcgctgacc gaacaccatc tggaaattca gggagaagcc
     2521 ttgcgcgatg ccccactggt aacgggcggt tctggtctgg cgattggcct ggcccggcag
     2581 tgggcgcaag aaaacggtaa ccaggctcgc gaagcagggc gtccgctcgc tgggcgcggc
     2641 gtagtgctct ccggttcatg ctctcaaatg accaaccgcc aggtggcaca ttaccgtcaa
     2701 attgcaccag cccgtgaagt tgatgtggca cgctgcctct caaccgaaac tctggccgct
     2761 tatgcacacg aactggcaga gtgggttctg ggccaggaaa gtgtacttgc tccactggtt
     2821 tttgccaccg ccagcactga cgcattggca gcaattcaac agcaatacgg tgcacaaaaa
     2881 gccagtcagg cagtagaaac tctgttttct caactagcgg cgcggttagc agcggaaggc
     2941 gtgacacgct ttattgtcgc aggcggtgag acctccggcg tagtcacaca gagcctgggc
     3001 ataaaagggt ttcatattgg cccaaccatt tcccccggcg tgccgtgggt aaacgcactg
     3061 gataagcctg tctcactcgc ccttaaatct ggcaacttcg gtgatgaagc ctttttttca
     3121 cgagcccaaa gagagttttt atcatgagcg atttcgcaaa agtagagcag tctttgcgag
     3181 aggagatgac gcggattgcc agttcattct ttcagcgcgg ctatgcaacc ggttcggctg
     3241 gcaatctgtc gctgctttta cctgacggga atttactggc gacaccgaca ggttcatgcc
     3301 tgggcaatct cgatccgcag cggctttcca aagtcgccgc ggatggcgaa tggttaagtg
     3361 gtgacaaacc ctcgaaagag gtgctctttc atctggcgct gtatcgcaac aatccgcgct
     3421 gtaaagcggt ggtgcatttg cacagcacat ggtcgacggc gctttcctgc ctgcaagggc
     3481 tggacagcag caacgttatt cgtccgttca caccatacgt ggtgatgcgg atgggaaatg
     3541 tcccgctggt gccttattac cgaccgggcg ataaacgcat cgcacaggat ctggcggaac
     3601 tggcagcaga caatcaggct tttttactgg caaatcatgg cccagtggtt tgcggtgaaa
     3661 gcctgcaaga agccgccaac aatatggaag agctggagga aacggcaaag ctgattttta
     3721 ttctcggtga ccgcccgatc cgttatctga ccgcaggtga aattgcggaa ttaaggagtt
     3781 aatgcaatgc ctcgttttgc agctaattta tccatgatgt tcaccgaagt gccttttatt
     3841 gaacgcttcg ccgcagcgcg aaaagccggg ttcgatgctg tggaatttct gtttccctat
     3901 gactactcca ccctgcaaat ccaaaagcaa ctggagcaaa accatctgac actggcgctg
     3961 ttcaataccg ctcccggaga tattaatgcg ggggaatggg gattatccgc ccttcccgga
     4021 cgtgagcacg aagcacacgc agacatcgac ctggcactgg aatatgcatt ggcgctcaat
     4081 tgtgaacaag tccatgtgat ggcaggcgtc gtgcccgctg gcgaagacgc tgaacggtac
     4141 cgggcagtat ttatcgataa tctccgctac gctgccgacc gctttgcgct acacggcaag
     4201 cgaatattag ttgaagcact cagccccgac gttaagcctc attatctctt ctccagccag
     4261 tatcaggcac tggctattgt tgaagaggtt gcgcgagata acgtgtttat tcaactagat
     4321 acttttcatg cgcaaaaagt agatggcaac ctgacgcatt taatccgcga ctacgctggg
     4381 aaatatgcgc acgtacaaat tgccggacta cctgatcgac atgaaccgga cgatggagaa
     4441 atcaactatc cgtggctgtt ccgcctgttc gatgaggtgg gatatcaggg ctggatcggt
     4501 tatgaatata aacctcgtgg cctcaccgaa gaagggcttg gctggtttga cgcctggcgc
     4561 taattcgtta aatccctaat tacaacgtac ccatacatcc ccccatctac ggatggggta
     4621 ggttaactat aactttcaga cagggtttcc catgtccaca attacattgt tatgcattgc
     4681 gttagctggc gtaatcatgc tgttgctgct ggtcatcaag gcaaaggtac aaccattcgt
     4741 tgctctgctc ctcgtcagcc tgttagtcgc acttgcggcg ggtataccgg cgggcgaagt
     4801 gggtaaagtg atgatcgccg ggatgggcgg tgtgcttggt tccgtcacta ttattattgg
     4861 tctgggcgct atgctggggc gtatgatcga acactctggt ggtgcagagt cactggctaa
     4921 ttatttcagt cgcaagttag gtgacaaacg aactatcgct gcgctgactc tggcagcgtt
     4981 cttcctcggt attcccgtct tctttgatgt cggctttatt attcttgctc caatcattta
     5041 cggttttgcc aaggttgcca aaatatcgcc actcaaattt ggcctgcctg tcgctgggat
     5101 catgctaact gtacatgtgg cggtaccgcc gcatccaggc cctgtcgccg cagcggggtt
     5161 actccacgca gacatcggct ggctaaccat catcggtatt gcgatttcta ttcccgtagg
     5221 gattgttggc tactttgcag cgaaaataat caataagcac caatatgcga tgtcggtgga
     5281 agtactggaa cagatgcaac tggctccggc cagtgaggaa ggcgcgacaa aattaagcga
     5341 taaaataaat ccaccgggcg tcgcgctggt cacctcgcta attgttattc ctatcgcgat
     5401 tatcatggcg ggtacggttt ccgcaacact gatgccgcct tcgcatcccc tgcttggtac
     5461 gctacagctg atcggctcac caatggtagc tctaatgatt gcgctggtgc tggcattctg
     5521 gttattggct ttacgtcgcg gctggagctt acaacacacc agcgacatta tgggctcagc
     5581 ccttcccact gcggcggtag tgattttggt tactggtgct ggaggggtat ttggcaaagt
     5641 gctggtggaa tcgggcgtcg gcaaagccct tgccaacatg ctacaaatga ttgacctgcc
     5701 tctgttacca gccgcattta ttatttcact ggcgctgtgt gcatcgcagg ggtcagcaac
     5761 cgtagcaatc ctgacaaccg gcgggttact ctcagaagcg gtgatgggat tgaatccgat
     5821 tcagtgcgta ttggtgacgc tggcagcctg ctttggtggg cttggggcgt cacatattaa
     5881 tgactcaggg ttctggattg tgaccaaata tctggggttg tcggtagcag acggtctgaa
     5941 aacctggaca gtgttaacga ccattctcgg ttttaccgga ttcttaatta cctggtgcgt
     6001 atgggcggta atttgacctt aataaaaagg tccgatgagc atcggacctt ttattgtgca
     6061 cagaaaaggc cagcctcgct tgagactggc ctttctgaca gatgcttact tactcgcgga
     6121 acagcgcttc gatattcagc ccctgcgttt gcaggatttc acgcaaacgg cgcaggcctt
     6181 caacctgaat ctggcgaaca cgttcacggg tgaggccaat ttcacgacct acatcttcca
     6241 gtgttgccgc ttcgtacccc agcaaaccga atcgacgtgc cagtacttca cgctgtttgg
     6301 cgttcagctc gaacagccat ttgacgatgc tctgcttcat atcgtcatct tgcgtggtat
     6361 cttccggacc gttctctttt tcatcggcca ggatgtccag caacgctttt tcggaatcac
     6421 cacccagcgg ggtgtctacc gaggtaatgc gctcgttaag acgaagcata cggctgacgt
     6481 catcaactgg cttatccagt tgctctgcga tctcttccgc acttggttca tggtccagct
     6541 tatgggacaa ctcacgtgcg gttcgcaggt aaacgttcag ctcctttacg atgtgaatcg
     6601 gcaaacgaat agtacgggtt tggttcataa tcgcccgttc aatcgtctgg cgaatccacc
     6661 aagttgcgta tgttgagaag cggaaaccac gttccgggtc aaacttctct accgcgcgga
     6721 tcagccccag gttgccctct tcgataaggt ccagcaacgc cagaccacga ttgccataac
     6781 ggcgggcaat ttttaccacc agacgcaagt tactctcgat catccggcgg cgagaggcga
     6841 catctccacg cagtgcgcga cgcgcaaaat aaacttcttc ttcggccgtt aacagtggtg
     6901 aataaccaat ctcaccaagg taaagctgag tcgcgtccaa cacacgctgt gtggctccct
     6961 gcgataacag ttcctcttcg gccaaatcgt tatcactggg ttcctcttct actaaggcct
     7021 tttcgtcaaa aacctcaact ccgttctcat caaattccgc atcttcattt aaatcatgaa
     7081 ctttcagcgt attctgactc ataaggtggc tcctacccgt gatcccttga cggaacattc
     7141 aagcaaaagc ctggttccgc cgatttatcg ctgcggcaaa taacgcagcg ggtttacgga
     7201 tttccccttg taacgaattt caaaatgcaa gcgtgttgaa ctggttccgg tgctacccat
     7261 ggttgctatt ttttgccccg ccttcacttc ttgttgttcc cggaccagca ttgtgtcgtt
     7321 atgggcgtag gcactcaggt aatcatcatt atgtttgatg ataatcagat taccgtagcc
     7381 gcgcagcgcg ttaccggcat aaacaacgcg gccatctgcg gtcgcgataa ttgcctgtcc
     7441 tttgctgcct gcgatatcaa tccccttgtt gcccccctca gaagcgccaa aggtttcgat
     7501 cactttgccc tcagtcggcc agcgccaggt ggagataggc gtactggttg atgtactgct
     7561 gacagtcggc tcggttgtgc ttgctgttgg taccgttaca ggcgctgtga ctgtggtcgc
     7621 agttggcttg ttgttcggca acattttgtt agcactctgt tcacccgaag actcagaata
     7681 cgtaattgtc ggttgcgacg caacagcaac ggtggaattt tgtgcaggct tgatcacaac
     7741 tccttgctct gctgcgtcgg cctgggtaat ggcatttccg ccagtgattg gcgtaccgga
     7801 agcattaccc acctgcaagg tctgaccaac gttcagcgcg tatggtgcct gaatattgtt
     7861 gcgctgagca aggtcacgga aatcgttgcc agtaatccag gcgatataga aaagtgtgtc
     7921 gccttttttc acggtatagg tactgccgct ataactgcct ttcggaatgt tcccatactg
     7981 acggttatag acgatgcgtc cgttttccat ctgtaccggc tgctgagcta ctggctgcac
     8041 tggctggatt tgcggttgtt gagtagcctg aatttgtggc tgctgtaccg gctgaatttg
     8101 cggttgctgg gctgtagacg tcgtccccat tttcggcggc ggcgtaatca acataccaga
     8161 attagtattt gcaggcgcat tgccattaac ggagctgacc ggtgccggtg gatttgaagt
     8221 gtcagaacag cctgccagcc atagcgaaac cagtgacaaa gcagcaatgc ggcgaacggt
     8281 gaattttggg cttcccgcgc tcatttatcc cccaggaaaa attggttaat aaccagtgac
     8341 ataattaccg tgcaaggcac cctactgaac actggaaaag atgttcacga tacgctgacc
     8401 tgcggcaaaa taaccaggaa aaatccaggt atttcctcac gttttaagcc agctcaccct
     8461 tcactaaagg gacaaagcgc acggcctcca cggtatcgat aataaattcg cctccccgac
     8521 gacgcacccg tttcaaatac tggtgctcct cccctacggg taagacgaga atcccgcctt
     8581 cgtccagctg cgtcattagc gcagttggaa tttccggcgg tgccgccgta acaatgatag
     8641 cgtcaaacgg cgcacgtgcc tgccaacctt gccatccatc gccatgacgg gttgaaacat
     8701 tatgtaaatc aagatttttc aggcggcgac gtgcctgcca ctgcaagcct ttaatccgtt
     8761 caaccgagca aacatgctgg acaagatgcg ctaggattgc cgtttgatat cccgaaccag
     8821 tgccaatttc cagcacccgc gactgcggcg tcagctcgag taattcggtc attcgcgcca
     8881 ccatatacgg ctgcgaaatt gtctgcccct gacctatcgg caaggcgata ttgtcccagg
     8941 ctttttgttc aaacgcttca tcaacgaatt tttcacgcgg cacggcggca agtgcattca
     9001 gcacctgctc atcctgaata ccttgcgcac gtaattgctc cagaagtgct tgtacgcgtc
     9061 tgcttaccat tgcgtgccaa ctcccacgct gtttaaccag tctgaaacca catcttgcgc
     9121 gctatgcgca gttaaatcca catgcagcgg cgtgatggag acatagccct catctaccgc
     9181 agcaaaatcg gtccccggac cggcatcaca tttaccgccc ggcgggccaa tccagtacag
     9241 cgtattgccg cgcggatctt gctgcgggat cacctgatct gccggatgtc gtgtaccgca
     9301 gcgcgtcacg cgaatacctt tgatttgatc caagggtaaa tccggaacgt taatattaag
     9361 aatacgtccg gtgcgcagcg gctctttaca cagtgcgcgc aaaattgaac aggttaccgc
     9421 cgcggcagtg tcgtaatgtt tatgcccgtc aagcgagacg gccagcgccg gaaaacctaa
     9481 atgacggcct tccatcgcgg cggctaccgt accggaataa ataacgtcat cccccagatt
     9541 cggcccggcg ttaatgccgg acacaacaat gtccgggcgc ggacgcatca gagcattcac
     9601 gccaagatag acgcaatcgg tcggggttcc catttgcaca gcaatatcac cattttcaaa
     9661 ggtaaacgtg cgcagggagg attccagtgt cagagaattt gaagcgccgc tgcggttacg
     9721 atcgggggcg accacctgaa cgtcagcaaa ctcacgcaag gctttcgcca gcgtttgtat
     9781 accgggtgca tgtaccccgt catcattact cagcaatatg cgcataatca cctgttgtgt
     9841 tgataagttc cctgacaacg ctggttgcaa aactacccgc cggaagccag aaacggatct
     9901 ctacggtgac gtcatcccac caattccagc ttaattgttg cggatacagc aacatcgctc
     9961 tgcgcgcggc ttcaactttt tcgcgcacca gtaaagcttg taattcagtt tctgcggcga
    10021 cagctgcctt ttcgaatgcc agcgcttcac gctgagttcc ccattcgcca ctacccggca
    10081 atgcggcggt tatcatcagc tctttatcgt tgacgcgacg ctgtaattcc gccagttctt
    10141 cggtggttgc gacaaaccag ctaccacgtc cggctaattg tagcgcatcg ccgtcaacaa
    10201 cttgattaac gtctgctttt ttgaggcgct cagcaacaat ctgattaaac aacgcactgc
    10261 gggctgccga caaccaaaaa ctccgtttat tgcgatcgcg caccggagta ttggtttgcg
    10321 cccagcgctg cgccccctgc aagttgctac cgccaatccc aaaacgttgg gcaccgaagt
    10381 agttcggtac accttttacg caaatatcga tcagacgttg ttcaacgtca tcgcgattgc
    10441 tcacttcgcg cagaaccagg gtaaaggcgt tacctttcag cgcgcctaaa cgcagcttgc
    10501 gcttgtgccg cgcatactcc agcacctggc agccttccag ttgaaaggcg ctcagatcgg
    10561 gcatttcctt gcctggcacg cgagcgcata accactgttc cgtaacagca tgtttgtctt
    10621 tttgcccagc gaagctgact tcacgggcat gaattttcag gaatttcgcc agtgcatccg
    10681 ccacaaaacg ggtattgcag ccgtttttga ggattctaac cagaatatgc tcaccttcac
    10741 catcaggctc aaagcccaaa tcttccacca ccacaaagtc ttccggattg gctttcagca
    10801 gcccggtgcc ttgcggttta ccgtggaggt aagtgagatt atcaaactca atcattttgt
    10861 tgccttaatg agtagcgcca ccgcttcaca ggcaatccct tccccacgtc cggtaaatcc
    10921 aagtttttcc gtagtagtgg ctttcacgtt aacatcatcc atatggcagc cgagatcttc
    10981 ggcaataaac acgcgcattt gtggaatgtg cggcaacatc ttcggtgcct gagcgataat
    11041 agtgacatcg acgttgccca gggtataacc cttcgcctga atacgacgcc aggcttcgcg
    11101 tagcagctcg cggctgtcgg cacctttaaa tgccggatcg gtatccggga acagcttgcc
    11161 gatatccccc agcgccgccg cgccaagcaa tgcatcggtc aacgcatgga gcgccacgtc
    11221 gccatcagaa tgcgccagca atcctttttc gtaaggaatg cgtacgccac caatgataat
    11281 tgggccttca ccgccaaagg catgtacgtc aaaaccgtgt ccaattcgca ttatgtattc
    11341 tcctgatgga tggttcgggt gaggtaaaac tcggccagtg ccaaatcttc cgggcgcgtg
    11401 actttaatgt tatccgcacg gccttcgacc aactgaggat ggaatccgca atattccagc
    11461 gccgaggctt cgtcggtaat agtcgcgcct tcatttagag cgcgcgtcag acagtcatgt
    11521 aacagctcac gagggaaaaa ttgcggcgtc agcgcgtgcc ataagccgtt gcgatcaacg
    11581 gtatgagcaa tggcattttt gcccggttcg gcacgtttca tcgtatcgcg cactggtgcg
    11641 gctaggatcc ctcccgtgcg gctggtttcg ctcaacgcca acaatcgcgc gaggtcatcc
    11701 tgatgcagac aaggacgagc ggcgtcatgc accaataccc actgcgcgtc gccagcggct
    11761 ttcaaacctg ccagcacgga atcggcacgc tcatcaccgc catctacaac ggtgatttgc
    11821 ggatgattcg ccagaggaag ttgtgcaaaa cggctatcgc caggacttat ggcaatgacg
    11881 acacgtttca cccggggatg cgccagcagc gcatgcaccg agtgttcaag aatggtttga
    11941 ttaccgattg agagatattg cttaggacat tccgtttgca ttcgacggcc aaatccggcc
    12001 gccggaacca cggcgcaaac atccaaatga gtggttgcca tgttaattcc cgggctgatt
    12061 tatcgattgt tttgccccgc agactgtgcg cgcttcgacg cgtcaggcac cagacgataa
    12121 aaagtttcgc ccggcctggt catgctgagt tcattacgcg cacgctcttc gagcgcctcc
    12181 tggccgccat tgagatcgtc aatttcggca aaaagttgat cgtttcgcgc tttaagtttc
    12241 gcgtttgtag cttgctgtgc cgccacatca tcattgacgc gggtatagtc atgtataccg
    12301 ttcttaccga accacagcga atactgtagc cagaccagaa tagccagcaa cagcagcgtt
    12361 agtttaccca tcctgccccc tgaaaaacgg catcatcatc ccatgcatcc gaagacgact
    12421 ctacatcctc tgttggggat accgcgacaa cgcgggcaaa tgtaccacat ttgtccattg
    12481 ttacgtatac ccagggcgtg cagaacataa tctcattatt agttacggtt tgaattatga
    12541 acagaggaga caagaaagta caaattagcc cagtagccac ataaacagtg cgccaaacat
    12601 aatgcctact gtcaccaggg tgaaaacaat actgtagcgt agctttccgt ccatcaatga
    12661 atgcagcgca atccccacca ctaccgcgac gggcatcagc gccagaaaga aaggccaggt
    12721 gtagataaag aagaacagcg tgttagagcc ataaatcaac atcggcatcg ccagcgcaaa
    12781 taaccaggag ataaaaccga ccacggcacc aggcagtgac catgtggttt cttcatcctc
    12841 agtaaggctg tcgttatttg ttagtgtaat gttatggcta ttacgcatat ttgatcctgt
    12901 tactttgacg aaccgggcat ggaaacccgg tggtgtctca ggatctgata atatcgttct
    12961 gtctcaacag atctaataat tgctgtacca aatttgttac taattgttca ccattgagat
    13021 gaatttctgc cgattcaggc gcttcgtaaa cggaatctat tcccgtaaag ttgcgcagtt
    13081 caccggcacg cgctttctta tataagcctt ttggatcgcg ggcttcgcaa atcgccagcg
    13141 gcgtatcgac aaacacttcg ataaagcgcc cttctcctac gcgttcacga accatctggc
    13201 gttcggcgcg gtgtggcgag ataaatgcgg tcagcaccac cagtccggct tcaaccatca
    13261 aattcgccac ttcaccgacg cgacggatat tctctttacg atcggcatcg ctaaaaccga
    13321 gatcgctgca taatccgtgg cgaacattat cgccatccag cagatacgta ctgacgccga
    13381 gtttatgtaa cgcctcctcc agcgccccgg cgaccgttga tttaccggac ccggagaggc
    13441 cggtaaacca cagcactaca ccacgatgac cgtggtgtag ctcgcgttgt tgcacagtga
    13501 ccggatggct atgccagacg acgttttcgt catgcagcgc cattatttat cccccagcaa
    13561 atcgcgcgcg ccccagtgcg gaaagtggcg acgaaccaga gcattcaatt ccagttcgaa
    13621 tgcactgaat tcagatggcg cagcagttgc ctggctaact ggctcgtgca ccataccggc
    13681 acccacggtc acattgctca ggcgatcgat aaaaatcagc ccacccgtca ccggattttg
    13741 ttgataacga tctaacacca gcggctcgtc aaaagtgaga tccacgaggc cgatcccatt
    13801 cagtggcagg ttttcaactt cacgctgggt aaggttatta atatcaacct gatagcgaat
    13861 gccatcaaca cgcgcgcgcg tcttcttacc ggcaattttg atgtcgtaac tctgccctgg
    13921 agaaagcggc tgttccgcca tccataccac atccaccgac gcgctctgca ccgccggtaa
    13981 cgcttcgtct gccgccagca gcagatcgcc acggctgatg tcgatctcat ccgtcagcac
    14041 cagggtgatc gcttctccgg caaaggcttc ttcgcgatca ccatcaaaag tcacgatccg
    14101 cgcgacgttt gattccacac cagagggcag cacttttaca cgttgcccga cttccacgcg
    14161 accggatgcc agcgttccgg cgtagccacg aaaatcgaga ttcgggcgat taacgtactg
    14221 caccgggaag cgcattggct gagcatccac cactcgctgg atctccacgg tttccagcac
    14281 ttcgagcagt gtcggaccgc tgtaccacgg catactttca ctttgcgatg ccacgttgtc
    14341 gccttccagt gcagagagcg gcacaaagcg gatatccaga ttacccggca gctgcccggc
    14401 aaaggtcaga taatcttcac gaatacgggt gaacgtctct tcactgtaat ccaccagatc
    14461 cattttgttg atcgccacga ccagatgttt gatccccaac agtgtggaga taaaactgtg
    14521 acgacgggtt tgatcgagca cgcctttacg ggcatcgatc agtaagatcg ccagttcaca
    14581 tgtcgatgcg ccagtcgcca tattgcgagt gtactgctcg tgccctgggg tgtcggcgat
    14641 aataaattta cgcttctcgg tagagaaata gcggtaggcc acatcaatag tgatgccctg
    14701 ttcgcgctca gcttgcaggc cgtccaccag cagagccaga tccagctttt cgccctgggt
    14761 accgtgacgc ttactgtcgt tatgcagcga tgagagctga tcttcataga tttggcgagt
    14821 atcgtgcagc aggcgaccaa tcagagtgct tttgccgtca tcgacgctgc cacaggtcag
    14881 aaaacgcagc aggcttttat gttgttgcgc aatcatccag gcttcgacgc cgccttcatt
    14941 ggcgatttgt tgtgcaagtg cggtgttcat cttaaaaata cccctgacgt tttttcagct
    15001 ccatagatcc cgcctggtcg cggtcaatca cgcggccctg acgttcactg gtggtggaaa
    15061 ccagcatctc ttcgatgatt tccggcagtg tttgtgcatt tgactccacc gcaccggtca
    15121 gcggccagca gcccagcgta cggaaacgca ccatccgttt tttaatcact tcgcccggtt
    15181 gcaggtcgat acggttgtca tcaatcatca tcaacatacc gtcgcgttcc agaaccggac
    15241 gttccgcagc gagatacagc ggcacaatgt cgatattttc cagccagatg tattgccaga
    15301 tatcctgctc ggtccagtta gagagtggga agacgcggat gctttcgcct ttgttaattt
    15361 gcccgttgta gttgtgccac agctccgggc gctgattttt cggatcccag cgatggaagc
    15421 ggtcacggaa agagtagatt cgctctttag cgcgggattt ctcttcgtca cggcgcgcac
    15481 caccgaaggc ggcatcaaaa ccgtatttgt tcagcgcctg tttcaggcct tcagttttca
    15541 taatatcggt atgtttcgcg ctgccgtgca cgaatggatt aatccccatc gccacgcctt
    15601 ccgggttttt atgcaccagc agctcgcagc cgtaggcttt cgccgtacga tcgcggaact
    15661 catacatctc gcggaatttc cagccggtat cgacatgcag caacgggaaa ggcagcgtac
    15721 ctggataaaa cgccttgcgc gccaaatgca gcatgacgct ggaatcttta ccgatagagt
    15781 agagcatcac cggatttgag aattctgccg ccacctcgcg aataatgtgg atgctttccg
    15841 cctccagttg ccgcaggtga gtaagtcgta tttgatccat aaccgttcct ttgcaatacc
    15901 gctattttct tgccatcaga tgtttcgact atagggagcg taagagaacg aatgaaatta
    15961 ccaattagaa tgagtagttc cttaacggaa taacgatttg gcaaagctaa tatcaaaaag
    16021 tgcttaaggc accggatttc tggcgtttag gaagatttga aattgtttta gcgcagcgga
    16081 agtttcatac tatggcggta aaaaaatttg catggtattt aaggactcac tatgttttcc
    16141 gcattgcgcc accgtaccgc tgccctggcg ctcggcgtat gctttattct ccccgtacac
    16201 gcctcgtcac ctaaacctgg cgattttgct aatactcagg cacgacatat tgctactttc
    16261 tttccgggac gcatgaccgg aactcctgca gaaatgttat ctgccgatta tattcgccaa
    16321 cagtttcagc aaatgggtta tcgcagtgat attcggacat ttaatagtcg gtatatttat
    16381 actgcccgcg ataatcgtaa gagctggcat aacgtgacgg gaagtacggt gattgccgct
    16441 catgaaggca aagcgccgca gcagatcatc attatggcgc atctggatac ttacgccccg
    16501 ctgagcgatg ctgacgccga tgccaatctc ggcgggctga cgttacaagg aatggatgat
    16561 aacgccgcag gtttaggtgt catgctggaa ctggcagaac gcctgaaaaa tacgcctacc
    16621 gagtatggta ttcgatttgt ggcgaccagc ggcgaagagg aaggaaaatt aggcgctgag
    16681 aatttactca agcggatgag tgacaccgaa aagaaaaata cgctgctggt gattaatctc
    16741 gataacttaa ttgttggcga taaattgtat ttcaacagcg gtgtaaaaac ccctgaagca
    16801 gtaaggaaat taacgcgcga cagggcgctg gcaattgcgc gcagtcacgg aatagccgca
    16861 acgaccaatc cgggtttgaa taaaaattat ccgaaaggca ctgggtgttg taatgacgca
    16921 gaaatattcg acaaagcggg cattgctgta ctttcggtgg aagcgactaa ctggaatctt
    16981 gggaataagg atggttatca gcaacgcgca aaaacagccg cattccctgc gggaaatagc
    17041 tggcatgacg taagactgga taatcaacag catattgata aggcacttcc tggaagaata
    17101 gaacgtcgct gccgtgacgt tatgcggata atgctacctc tggtgaagga gttggcgaag
    17161 gcgtcttgat gggtttgaaa atgggagctg ggtgttctac cgcaggggcg gggaattcta
    17221 agtgatatcc atcatcgcat ccagtgcgcc cggtttatcc ccgctggcgc ggggaactct
    17281 tactgcttgg tatgcggaat cacaccctga acggtttatc cccgctggcg cggggaacac
    17341 actaagcata cata
//
LOCUS       DAZOYL010000062        17342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZOYL010000062 DAZOYL010000000
VERSION     DAZOYL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.062"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            34..663
                     /locus_tag="ACHUOY_001500"
     CDS             34..663
                     /locus_tag="ACHUOY_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR0682314.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHUOY_001501"
     CDS             complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHUOY_001501"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR0682315.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHUOY_001502"
     CDS             1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHUOY_001502"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR0682316.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3031..3228)
                     /locus_tag="ACHUOY_001503"
     CDS             complement(3031..3228)
                     /locus_tag="ACHUOY_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0682317.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHUOY_001504"
     CDS             complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHUOY_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR0682318.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHUOY_001505"
     CDS             complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHUOY_001505"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR0682319.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHUOY_001506"
     CDS             complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHUOY_001506"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR0682320.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHUOY_001507"
     CDS             complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHUOY_001507"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR0682321.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHUOY_001508"
     CDS             complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHUOY_001508"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR0682322.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHUOY_001509"
     CDS             complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHUOY_001509"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR0682323.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            10899..11864
                     /gene="fepB"
                     /locus_tag="ACHUOY_001510"
     CDS             10899..11864
                     /gene="fepB"
                     /locus_tag="ACHUOY_001510"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0682324.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(11945..13195)
                     /gene="entS"
                     /locus_tag="ACHUOY_001511"
     CDS             complement(11945..13195)
                     /gene="entS"
                     /locus_tag="ACHUOY_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR0682325.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13306..14310
                     /gene="fepD"
                     /locus_tag="ACHUOY_001512"
     CDS             13306..14310
                     /gene="fepD"
                     /locus_tag="ACHUOY_001512"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR0682326.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14307..15299
                     /gene="fepG"
                     /locus_tag="ACHUOY_001513"
     CDS             14307..15299
                     /gene="fepG"
                     /locus_tag="ACHUOY_001513"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR0682327.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15296..16111
                     /gene="fepC"
                     /locus_tag="ACHUOY_001514"
     CDS             15296..16111
                     /gene="fepC"
                     /locus_tag="ACHUOY_001514"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0682328.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16108..17241)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUOY_001515"
     CDS             complement(16108..17241)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUOY_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR0682329.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4066 a         4939 c         4516 g         3821 t
ORIGIN      
        1 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
       61 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
      121 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
      181 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
      241 ccacccgaaa aaaaactgct gcaaaaatcc attgaaatag atggttttac ccaacccatt
      301 gtggtcacac atacggataa aaatgccatg gaaatcgtgg atggttttca ccgccatgaa
      361 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
      421 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
      481 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
      541 gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa
      601 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
      661 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
      721 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
      781 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
      841 cgcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
      901 ttcatttaag gcatttacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
      961 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     1021 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     1081 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     1141 tgcccgctca cgcagctgcg aatgcgccag cacactggca tggccctgtt gtgaaaagtt
     1201 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     1261 caaagcggcg aaatcagcct gctgccagac agttgcactg gagttatgcg gagtgttgag
     1321 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     1381 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcgggggc
     1441 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     1501 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     1561 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     1621 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     1681 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     1741 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     1801 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     1861 ctataacacg atgctagtat ttggcaatca agacgtttag atatctaaat aaaacaataa
     1921 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     1981 tcacatccag gaagtttcaa tcacctgaac gattttttca ctgatgaaca actttctcgc
     2041 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     2101 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     2161 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     2221 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgtt
     2281 ccgacgatcg ccgctacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     2341 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaacca
     2401 gagattatcc tcaacgcgcc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     2461 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     2521 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     2581 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     2641 attgctggtg gtgggatggt cggtgatctg ggcgatcgtt ttacgcgtgt ggcggcggct
     2701 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     2761 aaagtcgctt atggcattct ggtacaaagc gccttgctag gtcaggatga tgtgctggcg
     2821 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaaatg
     2881 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     2941 tacattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     3001 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     3061 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     3121 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     3181 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     3241 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     3301 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     3361 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     3421 cttttgcctg cgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     3481 tcgccgttgg cttcggatct ttcaatgccg ccagtgccga cttaatcgag aacagcgcca
     3541 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     3601 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tctatcattg
     3661 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     3721 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     3781 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     3841 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     3901 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     3961 ggttagcagg cagtgaatcg gtccgcttca ggccaggaga caccacgccc agcagatcct
     4021 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     4081 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     4141 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     4201 actgttcacc cacttcgctg gcaatctggt tgagcgtatc cggagtaatc gcaaagcccc
     4261 agctactcac cacctgcgcg gcagaagcga ccacatccgc cgtccctgcc ggagccagca
     4321 ccgccatcgg gctgttcatg gcgaagtaca cgcccggatc gatgatacag gcggaaacca
     4381 gtgccataat cgccacgaag gattccatta acattccacc gtagccgata aagcacgcct
     4441 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagcctg
     4501 acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa cagattaccg gtccataccg
     4561 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcgga cgcataatca
     4621 aaatgcctac cgccagacca acgatagtcc cgattttcag gaaggtagag aggtagtcac
     4681 gcggggccag cagtaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     4741 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcggg ctttctgcca
     4801 cccagccgcc agagataatg gcaaaaataa ggaacaccag gccaatcact gacacttcgc
     4861 caatacgccc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     4921 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     4981 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     5041 cagttggccc catctcttct ttgaccagct cacccagcga gcgaccgtca cggcgcgtag
     5101 aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc
     5161 agatcatccc cggcaggtag cccatttgcg ctgccagcac cggccccacc agcggccctg
     5221 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     5281 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     5341 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     5401 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     5461 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     5521 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacgat
     5581 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     5641 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     5701 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     5761 tgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     5821 tgccttcaga catcgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     5881 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     5941 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     6001 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     6061 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     6121 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     6181 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     6241 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     6301 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     6361 gccgctaacc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     6421 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     6481 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctgaaaca ggttaaacgc
     6541 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     6601 gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa
     6661 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     6721 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcttc
     6781 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     6841 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     6901 gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc
     6961 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     7021 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttcctggcg
     7081 gggacaggtg ctggcagtaa tccttcagtc atcaccaccc ggccagaccg cccggccaca
     7141 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     7201 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     7261 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     7321 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     7381 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     7441 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg
     7501 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     7561 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     7621 gcggcacgtt gcggttcaaa ggcccagtca actttattct gcggaatatc gtgagactcc
     7681 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     7741 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     7801 aagtgaatcc acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     7861 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct
     7921 tttttcgccc atcagctcat cttccatgct caccagtgcg gcatagatca ccgccggatg
     7981 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg
     8041 atctttctcg cgcccctgca cggtgatgta accctctgga tcaatagaga tcagatcgcc
     8101 ggaacagtaa aaaccgttgg catcaaaggc actggcattg tgctgcggac ttttgtaata
     8161 accgcggaag gtgtacggcc cgcgcgtcat caggcgtcca acttcccctt gcggcagtgg
     8221 atttccttca gcatcagcaa cccatacttc atcgtccgga cacattgggt aaccctgggt
     8281 atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcaccagcc cttccgccat
     8341 gccaaacacc tgctgcaact ggcagccaat ctcagcggaa atacgcgccg caagggtggc
     8401 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     8461 gccttcagcc agcgcctgca accacaggct aactgccggc ggcaccagcg cggtaacgtt
     8521 aacctgatgt tttgcaatca acgggaagca aagcgtggcg ctgggatcgg ccgccagaac
     8581 aaccgttcct ccggcaagaa agacgcccag cgatcctggc gaactcatag cgtagttatg
     8641 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
     8701 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagcttcg gtgtaccggt
     8761 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tagcagtaaa
     8821 accgtctgcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag
     8881 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
     8941 aaacagcgca tgttggcgat cggcaatcag taacgccggt tcaatctggc tggcataggc
     9001 gttcagttca ctacgctgat ggctgaacaa cgccagcacc ggcgcaacgc ccagtttcag
     9061 cagtgcgaaa aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc
     9121 accgggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
     9181 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
     9241 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
     9301 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
     9361 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
     9421 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
     9481 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
     9541 gccaaacagt tcgcggtcaa acggctccag ttcagcaata acctgggtcg cggcctgatg
     9601 cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc
     9661 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
     9721 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
     9781 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
     9841 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
     9901 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
     9961 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    10021 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    10081 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    10141 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    10201 acagtgggct gaggtctgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata
    10261 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    10321 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    10381 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    10441 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    10501 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    10561 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    10621 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    10681 cattattgat gaattcgcat aagcgcaatg tgatgtcctg cgccgttttg ccccctctcc
    10741 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    10801 accctaaccc tctccccaaa ggggcgaggg gacggattgt gcgctttgtc gaatttgtca
    10861 ttacgccctt aaccttatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    10921 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    10981 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    11041 gtattgtttc caccagcgtc accctgaccg gatcactgct ggcgattgat gctccggtga
    11101 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcaga tgaccagggc tttttacgcc
    11161 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg
    11221 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    11281 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    11341 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    11401 aagcggcaga gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca
    11461 aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc
    11521 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    11581 agttgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    11641 ttggtgggga aaatctggct gctgggttaa acggtgaatc gctattcctg ttcgccggtg
    11701 atcagaaaga cgccgatgcg atttatgcca atccactgct cgcgcacctg cctgcagtac
    11761 aaaacaagca ggtttatacg ctgggaaccg agacgttccg cctggattac tacagcgcca
    11821 tgcaagtgct ggacagactt aattcactgt taaaaaaaga ttgataaaac atacaattag
    11881 agcactaata atcccctcgc ccctttgggg agagggttag ggtgagggga aaatgcacct
    11941 ttagctaact gtcagacgct gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca
    12001 ccagcaccag caataacaac acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg
    12061 aagcaaccgg tgtcatcatc gcgcccaaac cacccaacag cgccgcgcct atggcatcgc
    12121 ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc taacatcgct tccggggttt
    12181 gcgtttgcag cattgtatat tgcagcaacg aactaaccgc gctcagccag ccgaacagcg
    12241 ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca atggcgagga
    12301 acgatcccag cgtggagagc agcatcaata accctggtcg cgcactatgt gccagcttcc
    12361 cgctggttaa cgcaccaata gccgcaccga gcgggatcgc cgcatagaga aaaccaatct
    12421 gtgccgccga catctgccag ttgtcagcca gcgccggata cagtacccgc accgcgctcg
    12481 ccatcgtcaa taaaccaccc agcaacgcaa tccctcctac cagcgggctg gcgagcagaa
    12541 aacgaaatcc tgccagtaat gatttcaacg gatgctcgcg cggctgcggt ggcggtggca
    12601 acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc gccagcccgt
    12661 agttccaggc tacgccgccg gtcgccagca ataaaccgcc aatcatgggc gaaatcaccg
    12721 accccagacg cacggtcaac atggtgatcg ccccggcctg cattaagttt tcacgcccta
    12781 ccagtgcagg tgttgccgcc agtagcgccg taacaccaag tgatgcgaaa aaaccatccc
    12841 ataaaccaag taaatagatt gccagcaatg acggctccgg cagcagcgcg ttcaggcaca
    12901 gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt ttgcgctcat
    12961 agcgatccgc cagcacaccg ccaaccatca ggccaacaaa catcgcgccg ccggtcagcg
    13021 tcaccgaaag ccccacctgc caggtagaat gcgtcatcat ctggatctgc accggcaccg
    13081 cgacgccgag caaacccaga gacacaattg agataaaacg agcgaggaat actgcgcgaa
    13141 acgccgggtg cgttttcaac aggctgaggt taagcagcca ggattgttta ttcattacaa
    13201 tgccttgcca tcaaatattt taaataccta ttcttaggct gcacatgcta acatatccaa
    13261 ataagatcga taacgacaat taatttcatt atcatggaag ttcgtatgtc tggttctgtt
    13321 gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat cgcgacggca
    13381 ttaagcctgc tcattggggc aaaatcactc cccgcgtcag tagtgctgga ggccttctca
    13441 ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg cgcgactgcc gcgcaccctt
    13501 gccggtttac ttgcaggcgg tgcgcttggg cttgctgggg cgttaatgca aaccctcacc
    13561 cgaaatccac ttgccgaccc cggcttgctt ggcgtgaacg ccggagccag ctttgccatt
    13621 gtgctgggtg cggcgctgtt tggttactca tccgcgcagg aacaactggc gatggccttc
    13681 gccggggcgc tggtggcctc attgattgtt gcctttatcg gcagtcaggg cggcgggcag
    13741 ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct ggaagggctg
    13801 accagcggca tcgccctgct taatcctgac gtctacgatc aactgcgttt ctggcaagcc
    13861 ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc ggttctgatc
    13921 gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca gtttgagcct cggcagtgac
    13981 accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt tgattggtct gctggcgatt
    14041 accgtgcttt gtggtagtgc gacggcggta gttggcccga ttgcctttat cggcctgatg
    14101 atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct gcccgtcacg
    14161 ctacttgcta cccctgccct gctgctgttt gccgatatca tcgggcgggt gattgtgccc
    14221 ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg caccggtgct gatcttcctc
    14281 gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc tcgccgatta ctcatcacct
    14341 gtttgctgct gatttccgcc tgtgtggttg caggtatctg gggattacgc agcggtgccg
    14401 tcacgctgga aatctcgcag gtattcgccg cgttgatggg cgatgcgccg cgcagtatga
    14461 cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg
    14521 cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc ggcagccctg
    14581 acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg
    14641 gtcaggacct gacggctatc gcgctggcag caatggtggg cggcattgtc acttctctgt
    14701 tggtctggct gctcgcctgg cgtaacggta tcgacacctt tcggttgatt attatcggca
    14761 tcggcgttcg cgccatgctg gtggccttta atacttggct gttgctgaaa gcgtctttag
    14821 aaacggcgct aacagcgggt ttgtggaatg ccggatcgct caatggcctg acgtgggcaa
    14881 aaacttcgcc ttccgcaccc atcatcatat tgatgctcat tgccgccgcc ttgctggtgc
    14941 ggcgaatgcg cttgctggag atgggcgatg ataccgcctg cgcgctgggc gtcagcgtcg
    15001 agcgttcgcg tctgttaatg atgctggttg cagtggtgct taccgctgcg gcaacggcgc
    15061 ttgccgggcc gatttccttt attgctttag tcgcgccgca cattgcccga cgcattagcg
    15121 gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg agcgctgtta ctgctggcgg
    15181 ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc gtcgttaccg
    15241 tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc aaaaaatgac
    15301 cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg ggatatggca aatataccgt
    15361 tgcggaaaat ctgactgtag aaattcctga tggtcacttc acggcaatta tcggaccaaa
    15421 tggctgtggt aaatccacgt tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg
    15481 gcatgtctgg ctggatggcg agcacattca acattacgcc agtaaagaag ttgcacgccg
    15541 gattggtctg ttggcgcaaa atgctaccac gccgggcgat atcaccgtgc aggagctggt
    15601 ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc tggcgcaaag aggatgaaga
    15661 agcggtaacg aaagcgatgc aggccacgag aataactcat ctggcagatc aaagcgtgga
    15721 taccctttct ggcggacaac gccagcgagc gtggatcgcg atggtgctag cccaggaaac
    15781 ggcaattatg ctgctcgacg aacccacgac ctggctggat atcagtcatc agattgatct
    15841 actggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg cggtgctgca
    15901 cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg aagggaaaat
    15961 tgttgctcag ggagcgccga aggagattgt cactgctgaa ctgattgagc gcatttatgg
    16021 tctgcgatgc atgatcattg acgatccggt agccggaacg ccgcttgtgg tgccgctcgg
    16081 acgaacggca ccttcaaccg caaatagtta aactaagtgg tctgccatca tggcatcctg
    16141 ttttctggat gccatcgcat agcgcaataa cacgctacca caagccacca tcccgccgat
    16201 cagcgcggaa aggatcacaa taatcgcctt acccggaccg tcttttttca ctggcaatga
    16261 cgggcttaac tgatatttaa acggcgtaaa attcacatcg ttgacatttg cttttgttaa
    16321 ttgctcgaca agatactgcc gattacgtaa ttcaccgttc agttccgcaa cgtcagtaac
    16381 cgctttttca atttccagtt tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc
    16441 ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc ctgccgcgtt
    16501 ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt
    16561 aatgcgatcc tgagccagtt tttctttttc aaactgggtt ttgatctcca gtttattacg
    16621 gacgttttct atcgactctt tcaccaccaa cgcagagata taatcgatat acccgctcaa
    16681 aacggtctgc gcctctttac tggttggcgc ggtaaaactt agcgtccagg aggtatacag
    16741 tgacggttca tcttttttct tactggcatt gtcatcaacc gctttcattt tttcgctcaa
    16801 tgcaacaata gcgcgatgca aatccagttc atcgattttc gcctctttta attggtccat
    16861 cacataaggt gatgaacgca ggtactcttc cagcaagcta acagactgaa acttcttgat
    16921 aaacaggtta aatgcaccgc cacggtcgat attaacatcg agatcaagaa cgcggagttt
    16981 ggtaaaggtt ttctccaggt cctgccactg aatggcttcg gcgggagtaa taacggctga
    17041 gctggtccat ttttgcggca ggatgaaaga gatcagtaaa cctgcgcagg caaatgcaaa
    17101 aacgaccgcc atgaccgttt ttttggcccg ccataaaact tcgattagat taagtaaatc
    17161 aatttcatta ttactgggcg acgccagagg atactcagaa aaatgagcgt cacttccctg
    17221 tttaatattc aatgatgaca tggggtataa cctgaaaatg agtccaatag gggaaaatct
    17281 ccacggcgct cactttacca gttggtgaga attatccgaa gctgaagttt gtaaattcct
    17341 tc
//
LOCUS       DAZOYL010000063        17225 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZOYL010000063 DAZOYL010000000
VERSION     DAZOYL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17225
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.063"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHUOY_001516"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHUOY_001516"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR0682330.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHUOY_001517"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHUOY_001517"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR0682331.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHUOY_001518"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHUOY_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR0682332.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHUOY_001519"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHUOY_001519"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR0682333.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="ACHUOY_001520"
     CDS             complement(5499..5696)
                     /locus_tag="ACHUOY_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR0682334.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHUOY_001521"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHUOY_001521"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR0682335.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHUOY_001522"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHUOY_001522"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR0682336.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHUOY_001523"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHUOY_001523"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR0682337.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHUOY_001524"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHUOY_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR0682338.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHUOY_001525"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHUOY_001525"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR0682339.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHUOY_001526"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHUOY_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR0682340.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHUOY_001527"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHUOY_001527"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR0682341.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHUOY_001528"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHUOY_001528"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR0682342.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHUOY_001529"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHUOY_001529"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR0682343.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="ACHUOY_001530"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="ACHUOY_001530"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR0682344.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="ACHUOY_001531"
     CDS             complement(17138..17191)
                     /locus_tag="ACHUOY_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0682345.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3882 a         4943 c         4331 g         4069 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatc
//
LOCUS       DAZOYL010000064        17017 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZOYL010000064 DAZOYL010000000
VERSION     DAZOYL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17017
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.064"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHUOY_001532"
     CDS             <1..266
                     /locus_tag="ACHUOY_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR0682346.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHUOY_001533"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHUOY_001533"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR0682347.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUOY_001534"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUOY_001534"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR0682348.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHUOY_001535"
     CDS             complement(1570..3270)
                     /locus_tag="ACHUOY_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR0682349.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQTLETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHUOY_001536"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHUOY_001536"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR0682350.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHUOY_001537"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHUOY_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR0682351.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="ACHUOY_001538"
     CDS             4828..6600
                     /locus_tag="ACHUOY_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0682352.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHUOY_001539"
     CDS             6593..8374
                     /locus_tag="ACHUOY_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0682353.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHUOY_001540"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHUOY_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR0682354.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHUOY_001541"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHUOY_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR0682355.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHUOY_001542"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHUOY_001542"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR0682356.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHUOY_001543"
     CDS             11336..11908
                     /locus_tag="ACHUOY_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR0682357.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHUOY_001544"
     CDS             complement(11939..12250)
                     /locus_tag="ACHUOY_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR0682358.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHUOY_001545"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUOY_001545"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHUOY_001546"
     CDS             12629..12982
                     /locus_tag="ACHUOY_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR0682359.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHUOY_001547"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHUOY_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHUOY_001548"
     CDS             complement(14736..15206)
                     /locus_tag="ACHUOY_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR0682360.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHUOY_001549"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHUOY_001549"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR0682361.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHUOY_001550"
     CDS             complement(16044..16262)
                     /locus_tag="ACHUOY_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR0682362.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHUOY_001551"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHUOY_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR0682363.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         4065 a         4123 c         4556 g         4273 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg tctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgat
//
LOCUS       DAZOYL010000065        16581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZOYL010000065 DAZOYL010000000
VERSION     DAZOYL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.065"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(150..302)
                     /locus_tag="ACHUOY_001552"
     CDS             complement(150..302)
                     /locus_tag="ACHUOY_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0682364.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(567..1301)
                     /gene="cysH"
                     /locus_tag="ACHUOY_001553"
     CDS             complement(567..1301)
                     /gene="cysH"
                     /locus_tag="ACHUOY_001553"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR0682365.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1376..3088)
                     /gene="cysI"
                     /locus_tag="ACHUOY_001554"
     CDS             complement(1376..3088)
                     /gene="cysI"
                     /locus_tag="ACHUOY_001554"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR0682366.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3088..4887)
                     /gene="cysJ"
                     /locus_tag="ACHUOY_001555"
     CDS             complement(3088..4887)
                     /gene="cysJ"
                     /locus_tag="ACHUOY_001555"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR0682367.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5206..5568
                     /gene="queD"
                     /locus_tag="ACHUOY_001556"
     CDS             5206..5568
                     /gene="queD"
                     /locus_tag="ACHUOY_001556"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR0682368.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5646..6917
                     /locus_tag="ACHUOY_001557"
     CDS             5646..6917
                     /locus_tag="ACHUOY_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0682369.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6908..7168
                     /locus_tag="ACHUOY_001558"
     CDS             6908..7168
                     /locus_tag="ACHUOY_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0682370.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7185..7760
                     /locus_tag="ACHUOY_001559"
     CDS             7185..7760
                     /locus_tag="ACHUOY_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR0682371.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7908..8768)
                     /locus_tag="ACHUOY_001560"
     CDS             complement(7908..8768)
                     /locus_tag="ACHUOY_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0682372.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8765..9544)
                     /locus_tag="ACHUOY_001561"
     CDS             complement(8765..9544)
                     /locus_tag="ACHUOY_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR0682373.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9522..10931)
                     /locus_tag="ACHUOY_001562"
     CDS             complement(9522..10931)
                     /locus_tag="ACHUOY_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0682374.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10953..12407)
                     /locus_tag="ACHUOY_001563"
     CDS             complement(10953..12407)
                     /locus_tag="ACHUOY_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR0682375.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12477..13293)
                     /locus_tag="ACHUOY_001564"
                     /pseudo
     CDS             complement(12477..13293)
                     /locus_tag="ACHUOY_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13611..14888
                     /locus_tag="ACHUOY_001565"
     CDS             13611..14888
                     /locus_tag="ACHUOY_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR0682376.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            14915..16400
                     /locus_tag="ACHUOY_001566"
                     /pseudo
     CDS             14915..16400
                     /locus_tag="ACHUOY_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3949 a         4361 c         4167 g         4104 t
ORIGIN      
        1 cattatgaat aatcaaataa ttttcatccg taatatatat gaataataaa cataggcata
       61 atattcacat aaataatata aattaaaaaa gggtgcagga gaccaaacca tcacaattca
      121 gtacgcgggg ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc
      181 ttaaactcca taccacgttc tctgatactc aactcacaca acgagtcgcc taccatcagc
      241 gtgaatccca gtactgtaaa acacagcact atgattgcca caagggcata ttttgtcagc
      301 atgtcttgcc tccctgcaaa gaagagacta acattcgaat tgtttgggtt cgaacgctgg
      361 cctcaggttg atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt
      421 aacgccagac agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg
      481 ttcacgttta aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttctgaca
      541 gaggcgttta atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag
      601 ccaaagaaac gtgtttcttc ttccgacatg ccaggttccc atttacgggt tgtatgggta
      661 tcaccgaccg ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc
      721 aggtactgat aaatagttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca
      781 cgctgaattg ccagcaccgg taaattggcg cgactgccgg attgttcacg gcgcaggcca
      841 gcaaaccagg tttgcgcgtt aagttctttc agagcccggt tcatcggttc gactttgttg
      901 atgtcattgt acttttcaat gccttcaacg ccctgctccc acagttttcc gtagcgtgct
      961 tcctgccagg ctgcgctttc ggtagcacgg tacactttca ggttgagctt gagtttgtcc
     1021 gttaactcgt caataaagcg gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc
     1081 accggaatat ccgggcgaat ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca
     1141 aagctggaag aaagtacata ttcaccgggc agattatcca gcgcccaggc tacgcggcct
     1201 tcagcgtcca gtttttccag ttcggcgtta gtttccgcca gcgccagaat acgatctacc
     1261 ttcggcagtt cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc
     1321 ttgcctgatg cgacgctcac gcgttcttat caggcctaca agaccgggct gatggttaat
     1381 cccacaaatc gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt
     1441 cgccgaagcc ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg
     1501 acgccaggat ttccggctcg gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa
     1561 tgcggttgcc gccaagatgc aggttgtagc gacccggcgc tttacccacc aggcccactt
     1621 ccgccagcat cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct
     1681 catcgctgac accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga
     1741 aacgctctgc ttccgccatc gccagcgggc aagtcgggaa tgacacgcag gccatcgagt
     1801 tttcacgctg cggcgtgacg gcattcatta acccgctctc tttggcgatc ttctcgatct
     1861 tcgctttctc gctttccggt acaccggtga tgatcagatt ctggttcgcc gtaatgcgga
     1921 aatcgccttt gtggatcttc gcgatctcca gcaggccggt tttcagcgga cgccccggat
     1981 aatcaaggat gcgaccattt tcgataaaca gcgtcaggtg ccagttatca tcaatgccct
     2041 taacccagcc aatacgatcg cctcgtccgg tgaactcata tggacggatc ggttcaaatt
     2101 tgatccccgc gcgacgctcc acttccgctt taaacgtctc aacccccacg cgctccagcg
     2161 tgtatttggt tttggcattt ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca
     2221 cgacggcttc ggccaccgcc agcgtatgct ccagcggcag atagccaaac tcgctcgccg
     2281 tgcgggcgta ggttttcttg ttgccgtgtt cgatagaaag cccaccgccc accagcaggt
     2341 tgaagcctac cagcttgccg ttttcggcga ttgccacgaa gttcatgtcg ttggcatgca
     2401 gatcgatatc gttctgcggc gggatcacta ccgtggtttt gaacttacgc ggcaggtagg
     2461 tctgaccaag gatcggttct tcatcagtag tggcgacttt ttcctggtcg agccagatct
     2521 ccgcatacgc gcgggtacga ggcaacagat gctcagaaat cttcttcgcc cactcgtacg
     2581 cttccgcgtg cagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt
     2641 cgttagctgt cgccagcgca tcaagaccga ccgagtgcag catctggtgc accggtttga
     2701 cgttcttttt cagaatgccg tggaactgaa acgtctggcg gttggtcagg cgaatgctgc
     2761 catagatggt gttttcaccg gcaaatttgt cgatcgcctg ccactgttta gtggtaatca
     2821 ccccacccgg cagacgacag cgaagcagca tcgcgtggcg cggctccagc ttctgttcag
     2881 cacgttcggc acggatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca
     2941 ggaagttgtc gcccttaaag ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc
     3001 cgcgcaggta gttgctttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca
     3061 ctaaaggccc tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac
     3121 gcgcagctca cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat
     3181 cacttccaga agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc cgcagacata
     3241 aatgtgggca ccgtcattga tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc
     3301 ttgtacataa actttttctt tttggtcgcg cgaccaggca agatcgatac gcgtcagcac
     3361 gccctctttg acgtagcgct gccactccac ctggtagagg aaatcttccg taaagtgcgg
     3421 gttgccaaag aacagccagt ttttacccgg cgcttcgtcg gcggcgcgtt gctgcataaa
     3481 ggcacggaac ggcgcgatgc cggtaccggg gccaatcata atcactggcg tttgcgggtt
     3541 agcgggcagg cggaaattgt cgttatgttc gataaatacg cggacttcgc cctcttcttc
     3601 cacgcgatcc gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta
     3661 acgcaccaca ccaacggtga cgtgtacttc gttctcgact tccgcctgcg aggaggcgat
     3721 ggaatacagg cgcggggtca gcgggcgcag caggttaatc agcgcttcgg catccagttg
     3781 cgccggagag aaacgcacca tgtcgacaat cggcgtggtc gcggcgtaat gctgtaactt
     3841 cgctttatcg cccaccagcg gcagcagcgt ttcgctgcgg gtaagcgtgg catagttctc
     3901 aacaatgttg gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg
     3961 caacgttttg ccctcgacgg tgacaggttc atcgcctttc agccacagca gttcgacaag
     4021 ttctttcacc agtgccgggt cgttctgata ccagacaccc agcgcgtcac ccggctggta
     4081 acgcaggcct gaatcgccta agtcaatttc gatatgacga acgtcttttt cagagttacg
     4141 cccggtaatt ttctggttaa ccgaaaggct cgccaccagc ggcgcgtctt tgctgtacgg
     4201 gctggtgtgg atttcattta ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg
     4261 cgcacgcgat ttaagcgcat caaccacgcg ggcgcgccac tcgctggcag cagcctggta
     4321 ttcaacatcg gcatcgacac ggtcgagcag gcgttcacca cccagttccg ccagcttgct
     4381 gtcgaaatct ttcccggact ggcagaaaaa ttcataagag ctatcgccga ggctaaacac
     4441 ggcaaacgcg gtgttttcca gctttggcgc ttttttggag aacaggaact tatgcagcgc
     4501 gacggcttct tccggcggtt ccccttcccc ttgcgttgac gtcactacga tgagcagttt
     4561 ttcgctggcg atttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt
     4621 tgctgctaat aaatcatcgc gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga
     4681 ggcggagata atagttatac ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc
     4741 agcaggctgc tgattgagta cgccccagaa atagccagaa acccaggcaa gctgggtagg
     4801 cgttaaatcg gtcgtggccg cctgaaggcg tgccagttgc tccgggttca acggaagcaa
     4861 cgcggaaggt gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc
     4921 tgcgccctgt cagcgcccat aaaacagaag agaagataag gttaacgggg tgaacggtgt
     4981 ggattaaaga cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag
     5041 tctttaacaa caaaatagat taaccaaccc aatgaaaaac aaatgaattt agccaatcat
     5101 taagataaat cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct
     5161 ttccggtact ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta
     5221 tttaaagatt tcaccttcga agccgctcac cgcttaccac acgtcccgga agggcataaa
     5281 tgtggtcgcc tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat
     5341 ccgcatacgg gctggattat cgatttcgct gaactgaaag cggcgtttaa gccaacttat
     5401 gagcgcctcg atcaccatta tctcaatgat attccaggtc tggaaaaccc aaccagcgaa
     5461 gttttagcaa aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg
     5521 atggtaaaag aaacctgcac cgcagggtgt atctatcgcg gcgaatgata agagtgtgtc
     5581 ggcggtcaat ttcccttaag taacgctatg ttagggtgtt gtgttctgga tatctggggc
     5641 atgacatgga agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt
     5701 gcgcgttacg ctgcgcgcga gcgggtttat ccgttttgtt actggaacgc gctgaaatcc
     5761 ccggcagcaa aaatctttcc ggcgggcggt tatataccca tgcactcgcg gaactcctcc
     5821 cgcaatttca tctgaccgcg cctcttgaac gacgcatcac tcacgaaagc ctttccctgt
     5881 taacgcccga tggcgtaacg acgttttcca gcttacagcc cggcggtgaa tcctggagtg
     5941 tattacgtgc acggttcgat ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat
     6001 gcatccccgg agcgacggtg gatgcactgt atgaagaaaa cggcagagtc tgtggcgtta
     6061 tttgtggtga cgatattctc cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg
     6121 tcctggctga acgtcacggg ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga
     6181 tcaaagaagt gctgtcgctg gaaacatccg ctattgaaga acgttttcat ctggagaata
     6241 acgaaggcgc agcgttgctg ttcagcggcg gaatctgtga tgacttaccc ggcggcgcat
     6301 ttctttatac taatcaacaa acgctctcgt tagggattgt ttgcccgctc tcttccctta
     6361 cgcaaagtcg tgttccggca agcgagctgc tgactcgctt taaagcgcat ccggcagtgc
     6421 gcccgcttat caaaaacacg gaatcactgg agtatggtgc gcatctggag ccagaaggtg
     6481 gcttgcacag tatgccggtg caatacgccg gtaacggctg gctgctggtg ggcgatgcgt
     6541 tgcgcagttg cgtcaatacc ggaatttccg tgcgcggcat ggatatggcg ctgactggcg
     6601 cacaggcggc ggcacaaacg ctaataagcg cctgccagca ccgcgagccg caaaatctgt
     6661 ttgcgcttta tcatcacaac gtagagcgca gcctgctatg ggatgttcta caacgttatc
     6721 agcatgttcc ggcgcttttg caacgccctg gctggtatcg ggcgtggcct gcgttaatgc
     6781 aggatatttc ccgcgattta tgggatcagg gtgataaacc tgttccaccg ctgcgccagt
     6841 tattctggcg tcatttacgt cgtcatggcc tgtggcatct ggcgggcgat gttatcagga
     6901 gtctgcgatg tctgtagccc ataatctctg gcgcgttgct gatgcgccgc acattgttcc
     6961 ggctgactcc gttgagcgtc agatggtaca acggttgatt aacgcctgtc cggcaggtct
     7021 tttttcgctc acaccggaag gtgacttacg cgttgattat cacggctgcc tggagtgtgg
     7081 cacctgccgt ttgctgtgcg acgaatccac actacaacag tggcgctatc cgccttccgg
     7141 attcggcatc acctaccgct ttggataaat aataaggata atttatgccc ctcttacacc
     7201 tactccgcca gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcta
     7261 tcgattctga atgccagttt atttccgtgt tgtacggcaa tatctgcacc atcagtaata
     7321 tcgttaaaaa gattaagaac gccgggaaat atgcttttat tcatgttgat ttgctggaag
     7381 gcgcgtcaaa taaagaagtc gttattcagt ttttgaaact ggtgaccgag gcggacggca
     7441 ttatcagtac caaagcctca atgttgaaag cggcaagagc ggaaggtttt ttctgtattc
     7501 atcgcctgtt tattgttgat tcaatttcgt ttcacaacat tgataagcaa gttgcgcaat
     7561 cgaatccgga ttgtattgag atcctgccag gcggtatgcc caaagtgctg ggctgggtga
     7621 cagagaaaat ccgccaaccg ctgattgccg gtgggctggt gtgcgatgaa gaagatgcgc
     7681 gtaatgcgat taacgcgggt gtcgtggcgc tttccaccac gaataccggg gtctggacgt
     7741 tagcgaaaaa attactttga cgggataatc gtaaccaatt gaatttggtt tgatttgtag
     7801 gccgcacgcc acatccgaca ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc
     7861 tacaagtccc caggccagaa ccgtaggcag gatcatccgg caacacgtta ttggcaatct
     7921 gcgtgaatgt ttgtcaccag tgcctccagc accactttcc agtcatcaac cacgccaaca
     7981 tctgcctgtg aaaataccgc agcgctggcg tcatgattaa tcgccaccac aaacttgcta
     8041 ttgcgtacgc ccgccattaa cgctgctgcg ccagaagcgc caacaacaat acacacctca
     8101 ggtgccagca gatgtccgga aataccaatc actttttcag catcaacgcc accgttcatc
     8161 actcgcgccc gactgtagcc cacttcggct cccagctttt ctgccagcat agcgatttcc
     8221 tggttatctg cctctccgcc ctgccccacc accagcaccc gtctggcctc agctaatggg
     8281 tcgcgagtaa catttttcag atcctctgtg ctaaccagcc agtccggaag cgcacccggg
     8341 acaatgttca gttgttgcat tccagagggc aacgtggcgt ttttcgctgc tcccgcctga
     8401 cgcgccaggg aaaggcatag cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt
     8461 ccccagtggg atttgcgcac gcttacggtt ggaatatcta acgaagtcac ctggcagatg
     8521 cttgcaccat gtaaacgcca ggccagccgg gtcgacagtt catcgccaaa cgttcccggc
     8581 ggaaagagaa caacgtccgc aggcgttcgc tgccactgtt ccaccaacgc atccagcacc
     8641 tgttccgcca ctacaggctg aggttcaatc tgccagtgcg ccagggtaca accgctgaaa
     8701 tcctgcgccg ccagccagct tgctattgcg gcattctcct gatttattgt cacaattgcg
     8761 atattcatgg ctgcatcctt tggcgtaaat agtcctgcca tagcttttga gctttttctg
     8821 ctaccgtttg cccatcaatc agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca
     8881 gacactgcat cgctggcatt tcggcagcaa cagttttacg gataatttct gctttccccg
     8941 cagccattcg ctggcgcatt cccggtactg gcaaggccac ttcaccgcac tgacgcacgg
     9001 caatcaccgc aggcaagcga acccggcaac aacgcaaccc atgttcagta cgctgttcga
     9061 gggtgataaa cagcgcgtcg agagtgaaac gctccacctg ggtgaagcag ggccagccca
     9121 gcatttccgc cagcaaaaat ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg
     9181 taattatcag atccagcgga ttctgatgct gccattcggc aatatggcga gcgacaaatt
     9241 ccggcgcaaa gcgcagatct gccgccgtct ccagcaatac agcttcctca aacccaagag
     9301 ccatgagata gcgcagccag tgcaatgccc gttcatcccc catgctcaac gcggttaaag
     9361 acatcggcgt accgtttttt ctctgcgcca acagcagcgc ggcagcggcc tgttcatcag
     9421 caccgagtaa acttcgcagt agcgaaatat ccggtccgct tttaccctga gccgccgcct
     9481 gccactcttt ttccgccagc attccggcat ccggttcggc tttaaacgct aacagaatgt
     9541 tcattcgcac ctcctacatt tcccgccgcc accagcggga gggctttagt ttctggtgcc
     9601 catagccagg tcacaacaaa atcaaccaac aacactgtcg ccaggagcaa taaggtgact
     9661 tgcattcccc actgcgccag cacccacggc agcaggccag tacttaccgc cgcgccaagt
     9721 cgactcatgg cggtggcaaa accgacgccc agcgagcgaa tgtcagtagg aaaactttcc
     9781 gcaggcaaaa tgcccaccag attactgact gccgaaatgg tggtgctgaa gagaacaaaa
     9841 agcagcagcg ttaatgaact gccggaaggc aaacaggcca tcactaccag cgttgccgcc
     9901 agcagcaaaa aacttcccag caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat
     9961 cccagcagcg cgcccacaat taacaacgca ttaagcatca ggctggcagt cagcgcatct
    10021 tccagaccaa tagtctgggc gatagttggc agccaggtat aaatcacaaa ccatgggatt
    10081 acgaggcaga caaagaagac gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac
    10141 aaggttttga tgtgtttatg ggtcgccgtt accacttcat cgcccagtaa aacatgggga
    10201 ccaaaatagc gatgcacgat agcgtgagct tctgcaaaac gcccctggcg cagtagccag
    10261 cgtggtgatt ctggcgttcc ccagcgtaat aacgtaatca acaacgcggg cagagctgcc
    10321 gatgccagta gccagcgcca ggcctccggg ttttcggaaa taaagtgatg tccggcaata
    10381 cttgccagca catagcctac ggtccacacc acgctgaatg cgcccagcaa aataccgcga
    10441 tggcggcgcg gggaaaattc agccagcaag gtgtgaccta ctgaataatc gcctcccaga
    10501 ccaatgccaa tcaaaatgcg cagtccaata agatgctctg gcgtggtggc aaaaaattgt
    10561 aaaaacgaag caagcgtaat cagcaaaaag ctgaaggtga agattttttg ccgaccaata
    10621 tggtcggaga tccacccaag aaccaggctg ccaaggaaca aaccaaggag cgccgagccg
    10681 ccgatcattc ccgccataaa cggcgtcagt tgcatggcgg gcgtaagctg aataatggcg
    10741 taaccaatga cgccgagaac ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca
    10801 atgcggcagt gaaaacggtt aagcggtaaa tcatccattc gcaccggtga agtgttcatc
    10861 tttacccctt cattgtccgg taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa
    10921 cgccccgtca tcgcggaacg ctgcctgaca cattattttt caatcggata gatagtaccc
    10981 gtattcataa tgccattagg atcgaactgc tttttcagcc cttccagcaa cgcccacgcg
    11041 ctgccgtgtt ccagtttgct ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc
    11101 atcgaaccgc cgaggcgaat ggtttcttca cagatgatct tgttgagcgg attgtggtac
    11161 ttgtcgattt cctcttccgg cttacagtca acgacgttgt aatcgtagac gaagtacatg
    11221 ttggtgccgt tctgatagct atgagaggaa tgaccgccca gcatggtgat gtcgtcggcg
    11281 tgcgggaact cagtacgaat acggttaata acgctttcgt agatttcgtg gatgcagctc
    11341 cagcagccgg acacttcagt ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt
    11401 tcggcagcca ctttatccgg tccccagttc aggttgttaa accaggtttc gatcagcttg
    11461 ctgtccacgc gctggcattg cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc
    11521 gccttcgcaa tgcgagggtt accttcagcc ataaagatca gcacgcattt tccgtctgca
    11581 aaatgggtga agtgttgggt gccatcttca gcgtcataca aacgagcgat cgacggacga
    11641 tacccttcca ccatgacttc acgcaggatg ttgaagtcgg ttttcatgtc ttccaggata
    11701 tagccgtaga agaggttgtt ttccggggtg aatttaaaga ttttcactgt tacttcagtg
    11761 atatagcaaa gtgcaccttc gttaccgatg atgatgtggc gaatgtccgg gccagccgcg
    11821 cggcgtggca cgtttttaat gcgtgtgacg gtgccatcag ccaatactgc ttccagacca
    11881 acgaccatat cttcgattgc gccgtagagt gtggagaact gcccgatact gcgggttgct
    11941 accaagccgc ccatctgcgc cagcggcttt gactgcggag aatgccccgt ggtgtaacct
    12001 ttttcacgca acgcattttc cagcacctcc agcggaaccc cacattgcgc cgtcgcctgc
    12061 atattctcaa tatcaatatt aatgatttga ttcatggcgg agccgtcaag caccaccgag
    12121 ttttccacaa cggtttccag cccaccttca gtggcggaag cgccggtacg cggcacaccg
    12181 ttaattttgt gcgcattcat aaaattcagc acacgggata cttgctctgt ggaaccgagt
    12241 tttacgaccg ctgccggaat cggcaaagta taaatgccat gaatatccgg aaatttacga
    12301 aaacggtcaa tactgttctt ctttaatact gtttcatcgg taattacgcg atctgcacca
    12361 acaatttcct ttagttggtc gacaatcgct gcgcgagata aagacatagt aattccttcc
    12421 tgataataaa aatggaataa tcattaataa gagaaaacag aaaccaccgt tatggattag
    12481 cgcactaaat aaccaccatt gaccactaat aaatgcccat tgacataatt tgaagccgga
    12541 cttgcgagga atacagctgc gcccattaaa tcctgagtat cgccccaacg gtttgccgga
    12601 atatgatcaa gaacgcgctg attggtttct ggattactgc gtgtcgccag cgtaatatct
    12661 gttgcataat agccaggagc aataccattt acctgaatat tatattgacc tagttcatca
    12721 caataagctt tggtgaatcc ggcaagagca tgtttagtgg cagaatatgc aggtgaccat
    12781 tgtccaccta agtaagagaa caatgaacag atattaataa ttttgccgct tttctgcggg
    12841 atcataattt ttgcagcttc atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc
    12901 atcggatccc agtcggcacg accgaagtcc agcaccttat tcagcttaca aataccggca
    12961 ttgtttacca gaatatcaac tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc
    13021 ggcgcgcctt ctgcggtgat atccacctgc atgaagtcca cctcaacacc ctgtttttca
    13081 atcatttcct ttgtttcgcc gttatctttc aatcatttcc tttgtttcgc cgttatcttt
    13141 gacgaaacta ggaataaaga tatttgcgcc agctttggcc aacgccatgg caaatgcctg
    13201 gcctaaaccg ctattcccac cggtaacaat tgcggtttta cctttcagtg agaaaaaatc
    13261 cattgagaac gcattgagag attcgattga catagttagc tccaatttcc gtaaggcaaa
    13321 aaaaagaaag gtaacttccc ccgcgagagg gaagttacct ttctcattat ctctggtaac
    13381 tggagataat ttttagcata acggcataac tgctaatgtg atcgtaatca cagtgtgata
    13441 ttcgtattgt caaatagttt taaaaagcag aagcgtgatt actctcacac aacacctctg
    13501 ctttccatgt tatttcttat tgcaattact agagacctga gaaaagtaat ccacctgaaa
    13561 gggcggctta cttttctctt ttttttgtct attacaaatg ggaatacgat atgcaacaca
    13621 actcatatcg ccgttggata accctcgcga taattagttt tagcggcggc gttagttttg
    13681 acctggctta tttacgttat atttttcaaa ttcccatggc gaaatttatg ggattcagca
    13741 ataccgagat aggtttaata atgagtacct ttggtattgc ggctattatt ctttatgccc
    13801 ccagcggcgt tattgccgat aaattttcac accgcaaaat gattacttcc gcgatgatca
    13861 ttaccggatt actgggtctg ttaatggcaa cgtatccacc gctgtgggta atgctctgta
    13921 ttcaggtcgc ctttgcgata acgacgattt taatgctgtg gtcggtgtcg attaaagccg
    13981 cctcgttgct gggcgatcat agtgagcaag ggaaaattat gggctggatg gaagggctgc
    14041 gcggcgtcgg tgtaatgtcg ctggcggtgt ttaccatgtg ggtcttttct cgctttgcac
    14101 cggatgacag cgccagcctg aaaacagtca ttatcatcta cagtgtggtt tacatcttgt
    14161 tggggattct gtgctggttt tttgttagcg ataacaacaa cctgcgcagt gccaataacg
    14221 aagaaaaaca gtcattccag cttagcgaca tccttgccgt tttgcgtatc agcaccacct
    14281 ggtattgcag catggtgatt tttggtgtct tcacaatcta cgccattctg agttactcca
    14341 ccaactatct gaccgaaatg tacggcatgt cgctggtggc agcgagctac atggggattg
    14401 tgatcaacaa aatcttccgc gcgctgtgcg gcccacttgg aggcattatc accacctaca
    14461 gtaaagtgaa atcccctacc cgcgtgattc aaatcctttc catcatcggt ttgctggcgt
    14521 tgacagccct gctcgtcacg aactctaacc cccaatcggt cgcgatgggg attggcctga
    14581 ttttactgct gggattcacc tgttacgcct cacgcgggct gtactgggcc tgccccggcg
    14641 aagcgagaac accgtcttac attatgggca ccacggtagg tatttgttcg gtgattggat
    14701 tcctgccgga tgtcttcgtt tacccgatta tcggccactg gcaagacacc ctgcccgctg
    14761 cagaagccta ccgcaatatg tggctgatgg gcatggcggc gctcggcatg gtgattgtct
    14821 ttaccttttt gctgttccaa aaaattcgta ctgctgatag cgcccccgca atagctagca
    14881 gcaagtaagc caggaaaatt acaggagaaa aatcatgtcg aaaaaataca tcatagggat
    14941 tgatggcgga agtcagagca caaaagtggt gatgtacgat ctggaaggta atgtggtttg
    15001 cgaaggtaaa ggcttattac agccgatgca cacgcctgat gccgatactg cagaacatcc
    15061 tgacgacgat ttatgggcat cattatgttt tgcaggtcac gatttgatga gccagtttgc
    15121 cgggaataaa gaagatattg ttggcatcgg cctgggctcc atccgttgct gccgtgcgtt
    15181 attgaaagcc gacggcacgc cagctgcgcc gttgattagc tggcaggatg cacgcgttac
    15241 acgcccttac gaacatacga atcctgacgt ggcgtatgta acctcttttt cgggttatct
    15301 gacgcatcgc ttaaccggcg aatttaaaga taatatcgcc aactattttg gtcagtggcc
    15361 ggtggattat aagacctggg catggagcga agatgccgca gtcatggaga agtttaatat
    15421 cccccgtcat atgctgtttg atgtgcaaat gccaggcact gttcttgggc atatcacacc
    15481 tcaagccgcg ctggcgacgc atttcccggc aggactgccg gttgtttgta ccaccagtga
    15541 taaaccggta gaagctctgg gagccggatt actggatgat gaaactgcgg taatttctct
    15601 tggcacttac atcgcattga tgatgaacgg caaagccttg ccgaaagatc cggtggcgta
    15661 ctggccgatt atgtcttcta ttccgcaaac attgctgtat gaaggttacg gtattcgcaa
    15721 aggcatgtgg acggtgagct ggctgcgcga catgttaggc gagtcgttaa ttcaggatgc
    15781 cagggcgcag gatctttcac cggaagattt actcaacaag aaagcctcta gtgtgccacc
    15841 tggctgtaat gggctgatga cggtgctgga ctggctgacc aatccgtggg aaccgtacaa
    15901 acgcgggatt atgatcggct ttgattccag catggattac gcatggatat atcgttcgat
    15961 attggaaagc gtggcgctga cgctgaagaa caattacgac aatatgtgta atgaaatgaa
    16021 tcactttgcg aaacatgtaa tcattactgg cggcggttcg aacaggatct gtttatgcag
    16081 atttttgccg acgtgttcaa ccttccggca cgccgtaacg ccattaacgg ttgtgcaagc
    16141 ctgggggcag cgattaatac aggggtaggt ctggggctat acccggatta cgcaacggct
    16201 gtcgataaaa tggttcgcgt gaaagatatc tttataccga ttgagagcaa tgccaaacgc
    16261 tacgacgcga tgaataaagg cattttcaaa gacctaacca aacatactga tgtgatcctg
    16321 aaaaaatcgt atgaagtgat gcatggggaa ttggggaatt ggggaatgcg gattcgatcc
    16381 agagctggtc gaatgcgtga ataaggtagg ccgggcggaa aactgcccgg cctgaacata
    16441 cctgaatggt tatccccgct ggcgcgggga acataagttc gacaacttaa aaaatgataa
    16501 caagtccggt ttatccccgc tgatgcaggg aacataataa aaactttaac tagttcggtt
    16561 tatccctgtt tttacaagga a
//
LOCUS       DAZOYL010000066        16356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZOYL010000066 DAZOYL010000000
VERSION     DAZOYL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.066"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            30..113
                     /locus_tag="ACHUOY_001567"
     CDS             30..113
                     /locus_tag="ACHUOY_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0682377.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(187..1389)
                     /gene="ubiI"
                     /locus_tag="ACHUOY_001568"
                     /gene_synonym="visC"
     CDS             complement(187..1389)
                     /gene="ubiI"
                     /locus_tag="ACHUOY_001568"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR0682378.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1412..2590)
                     /gene="ubiH"
                     /locus_tag="ACHUOY_001569"
                     /gene_synonym="visB"
     CDS             complement(1412..2590)
                     /gene="ubiH"
                     /locus_tag="ACHUOY_001569"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR0682379.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2587..3912)
                     /gene="pepP"
                     /locus_tag="ACHUOY_001570"
     CDS             complement(2587..3912)
                     /gene="pepP"
                     /locus_tag="ACHUOY_001570"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR0682380.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3938..4516)
                     /gene="ygfB"
                     /locus_tag="ACHUOY_001571"
     CDS             complement(3938..4516)
                     /gene="ygfB"
                     /locus_tag="ACHUOY_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR0682381.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4684..5013
                     /gene="zapA"
                     /locus_tag="ACHUOY_001572"
     CDS             4684..5013
                     /gene="zapA"
                     /locus_tag="ACHUOY_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR0682382.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            5055..5238
                     /gene="ssrS"
                     /locus_tag="ACHUOY_001573"
     ncRNA           5055..5238
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUOY_001573"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5259..5861
                     /locus_tag="ACHUOY_001574"
     CDS             5259..5861
                     /locus_tag="ACHUOY_001574"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR0682383.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5962..6021)
                     /locus_tag="ACHUOY_001575"
     CDS             complement(5962..6021)
                     /locus_tag="ACHUOY_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0682384.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6353..6412)
                     /gene="ibsC"
                     /locus_tag="ACHUOY_001576"
     CDS             complement(6353..6412)
                     /gene="ibsC"
                     /locus_tag="ACHUOY_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR0682385.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6641..7873)
                     /gene="serA"
                     /locus_tag="ACHUOY_001577"
     CDS             complement(6641..7873)
                     /gene="serA"
                     /locus_tag="ACHUOY_001577"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR0682386.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8129..8788)
                     /gene="rpiA"
                     /locus_tag="ACHUOY_001578"
     CDS             complement(8129..8788)
                     /gene="rpiA"
                     /locus_tag="ACHUOY_001578"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR0682387.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            9218..10111
                     /gene="argP"
                     /locus_tag="ACHUOY_001579"
     CDS             9218..10111
                     /gene="argP"
                     /locus_tag="ACHUOY_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR0682388.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10314..12458
                     /gene="scpA"
                     /locus_tag="ACHUOY_001580"
     CDS             10314..12458
                     /gene="scpA"
                     /locus_tag="ACHUOY_001580"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR0682389.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12451..13446
                     /gene="meaB"
                     /locus_tag="ACHUOY_001581"
     CDS             12451..13446
                     /gene="meaB"
                     /locus_tag="ACHUOY_001581"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR0682390.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13457..14242
                     /gene="scpB"
                     /locus_tag="ACHUOY_001582"
     CDS             13457..14242
                     /gene="scpB"
                     /locus_tag="ACHUOY_001582"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR0682391.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14266..15744
                     /gene="scpC"
                     /locus_tag="ACHUOY_001583"
     CDS             14266..15744
                     /gene="scpC"
                     /locus_tag="ACHUOY_001583"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR0682392.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15741..>16356)
                     /locus_tag="ACHUOY_001584"
     CDS             complement(15741..>16356)
                     /locus_tag="ACHUOY_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0682393.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         4000 a         4391 c         4154 g         3811 t
ORIGIN      
        1 tgaataaggc aatattaaga gctaaaaaaa tgtcaaaaaa cactaaatca aaaaataatg
       61 acattagaaa atataatgcg aaaacggagg tgaaattagt ttatttcaaa tgaggaaaat
      121 ctcccggcga aaaaaccggg agatgaaagt gtgatgggta tcaaataaac agcagaagag
      181 aaatttttaa cgcagccatt caggcaaatc gtttaatccc attgcctggc ggataagttg
      241 tggtttaacg ccaggaagcg tgtcggccag tttcaaacca atatcacgca gcagtttttt
      301 cgccggattg gtaccggaaa acagatcgcg gaatccctgc ataccagcca gcatcagcgc
      361 cgcactgtgc ttgcggctac gctcataccg gcgcaggtaa atgtactgcc cgatgtcttt
      421 cccctgacga tgcaaccgtt tcagttcagc aatcagctct gcagcatcca taaagccaag
      481 atttactccc tgccccgcca gcgggtgaat ggtatgcgcg gcgtcgccca ccagcaccag
      541 acggtgcgcg gcaaactggc gcgcataacg ccccgtcagt gggaacacct gacgcgcgct
      601 ctcaaccttg cataagccca ggcgattatc aaaagcgata tttaacgcgc gattaaattc
      661 atcttcactt gcctgctgca tccgctgcgc ttcctctggc gacagtgacc agacaattga
      721 gcaaagatgc ggatcgctaa gcggtaaaaa ggccagaatg ccttcgccat ggaaaacctg
      781 ccgcgccacc gcatcatgcg gttcttccgt gcgaatggtc gctaccagcg cgtgatgctg
      841 ataatcccag aaagtcagcg gaatatctgc tttgttgcgc aaccaggaat tagcaccgtc
      901 cgcgccaatc accagacgcg ccgttaacat gctgccatct ttcagcgtca ggaaagtttc
      961 attttctccc caggcgacct gctgtaattc tgcgggggcc aacagagtga tatctgacga
     1021 ctgctgcgct ttgttccaca gcgcgtagtg aatcactgag ttttcaacga tatgcccaag
     1081 atggctatag cccatgcttt gatcgtcaaa cgaaatgtga ccaaaactgt ctttgtccca
     1141 cacttccata ccgtgataac aactggccct acgagagaga atgtcctgcc agacgccaag
     1201 acgggtgagt aatttttcgc tggcggcatt gatagccgaa acgcgcagtt gtggtggtgc
     1261 atccgccgcc agaggttccg gtacgcgctg ctccagtacg gcaacgcgta agccgctccc
     1321 ctgtaagccg caggcaaccg ccagccccac catgccgcca ccaacaatgg ctacatcaac
     1381 actttgcatt gtttattcct taaaaccgcc ttcaacgcgc cacccaaccg agggtgcgct
     1441 gcgccagcac atcgcgtgcc ggggtgaata attccatcgt catcagtccg atgttgcgcc
     1501 cgacaaccag cggtgcccaa cggttggcaa aaagatgtac caggctgtcc gtgacgccga
     1561 tggttgcttc gcgatcgctc tgtcgacgct gctgatagcg gcacaatatg ccgtaatcac
     1621 cgatgtcttc tccgcgctca tgcgcctgag tcagggtttc cgcaaggctc atgacatctc
     1681 gcataccgag gttaaaccct tgcccggcaa tcgggtgcag agtttgcgcc gcattgccca
     1741 ccagcacggt acgatgggta atagctctgg cggcgcgggt taacgccagc ggataagcac
     1801 tgcgtttacc ggcgtgggta atttttccaa gtcgccagcc aaaggccgac tggagttcac
     1861 ggcaaaactt ctcgtcgctc cacgacagca cctcttcgcg ccgttccagt ggatgacacc
     1921 agaccagcga acagcgtccg tcagacatcg gcaacatcgc cagcgggcca tgttgcgtga
     1981 agcgttcaaa agcgcggcct tcgtgcgcaa cggaagtcgc aacgttggca atcacggcca
     2041 gttgttcgta aggctcctgc tgccagtcaa cgccgcacgc ggtggctaac gctgaatggg
     2101 tgccatcagc tgctaccagc acgcggcccg tcagcgtctc gccactctcc agcgtcactt
     2161 caacgtgact ctgagtacgg gcaacgttag ccacgcgatc agggcaatgc agcgttacgc
     2221 caggtgcttt acgcagcaat gcaaacagcc gttgcccgac attgtgcaat tcgacaacct
     2281 gtcccagcgc cgccagttgg taatcttctg cggcgagggt gacaaatcca gcgtgaccac
     2341 gatcgctgac atgcacggta gtgatggcgg ttgcgtaatc cgccagagat tgccagacgc
     2401 cgatgcgcgc cagttgctga caggtccccg cagccagcgc aatagctcgt ccatcaaagc
     2461 ccggatgagc atgtgactct ggcgcggtcg cttcaatcaa atgtaccggc agcgccccgt
     2521 gacttaaccg ggaaatagcc agcgccagcg tcgcgcccgc catgccgcca ccgacgatga
     2581 ttacgctcat tgctttctcg cagctgccat caacgcttcg atttcttccg gctttttcac
     2641 cacgctggcg gtgaggtttt cgttgccggt ttcggtaatc acaatgtcgt cttcaatacg
     2701 aatgccgata ccgcgatatt gttctggcac atccgcatcc ggcgcgatat acagaccagg
     2761 ctctacggtc agtaccatcc ccggttccag aatgcgcgag cgatcctgac cataaacgcc
     2821 aacgtcatgg acatccagtc ctaaccagtg gctaaggcca tgcataaaga aaggacgatg
     2881 ggcgttctga gcgatcagtt catcaacatc acctttcagg atgccgagtt ttaccaggcc
     2941 gctaaccatg atgcgcacca cttcaccagt gacttccaga atggaagttc ccggacgata
     3001 caggcgcagg ctggtttcga gagactccag cacaatgtcg tagatttcac gctgggcctg
     3061 ggtgaatttg ccgttgaccg ggaaggtgcg ggtaatatcg cccgcgtaac ctttgtattc
     3121 acaacccgcg tcaatcaaca ccaggtcacc gtcgcgcagt tcactctcgt tttcggtgta
     3181 gtgcagaatg cagccgtttt caccgctgcc gacaatggtg ttataggacg gatagcgcgc
     3241 accgtggcgg ttaaattcgt ggtgaatttc gccttccaga tggtactcga acattcccgg
     3301 acggcatttt tccatcgccc gtgtatgtgc catggcggtg atttctcccg cgcggcggag
     3361 tacggcaatc tcttctggcg atttgaacag gcgcatttca tgaacaacag gacgccagtc
     3421 gatcatcgtt gccggtgcgg tgagattttg ccgcgaacct ttacgcagtt tttccagcgc
     3481 actgttcatg atttcatcag catatgcata ttcgccctgg gcatggtaaa ccacatccag
     3541 gccgttaagt agttgataaa gttgctgatt gatttcgctg aatgccagtg cgcggtcaac
     3601 gcccagtttc tctggcgcgg catcctggcc taagcgacgg ccaaaccaga tctccgccgt
     3661 caggtcgcga acgcggttaa acagaacgct gtggttatga gtgtcatcgc ttttaatcag
     3721 caccagcacc gcttccaatt cgttaaagcc ggtgaagtac cagaagtcac tgttctgacg
     3781 ataggggtat tcgctgtcgg cgctacgtgt tacttctggt gcagcaaaaa tcagcgcggc
     3841 gctgccgggt tgcatttgct ccaccagggc ctgacggcga cgctgaaact cttgccggga
     3901 tatctcactc ataacactct ccttacgttt tttgttttta gtgtagagtc ggtttttgta
     3961 cttctggcgc ggtcggttgc ggatgagtaa aggtgtcgtg gcataacagc gcggcaacac
     4021 gaacgtattc gatgatctct tcaagcgaca tttcaagctc ttcctgatct tcgtcttcgt
     4081 cgtaacccag ttgcgcaatg ttacgcagat cgtcgatagc ttcaccggtt tcgccggtca
     4141 ctttatccag cttcggttgc gtaacgccaa gaccaagcag gaagtgattg acccaacctg
     4201 ccaatgcatc agcccgatcg aaaacgctga catcatcgcc atcaggcaga taaagctgaa
     4261 aaaggaagcc gtcatcctgc agggcatcgc tggtggcaga gtgcatttta cgcagtgcct
     4321 gtgccagctc atgaccgaaa gccatgcctt cgttcgtcag gtcgtgaagt agcggcagcc
     4381 atgagctgtc atcgttaccg ccacatatca tcccactgat taaaccatgc atctcggcag
     4441 gggtcagccc cgtcccttgt tggttcagat actggttcat ttcgttgtaa ccaggcattt
     4501 cgttctgtat agacataagc attcgtcgtc aaagggagga atattcatga tatgctacca
     4561 ctttgggccc tggtggacca gaaaagggct tgtctcttct catcagggta gctatagtgt
     4621 cgccccttcg cagaccatgg gtctaaagac gaaggcagcg cagtcaatca gcaggaaggt
     4681 ggcatgtctg cacaacccgt cgatatccaa atttttggcc gttcactgcg tgtgaactgc
     4741 ccgcctgacc aaagggatgc gttgaatcag gcagcggacg atctgaacca acggttgcaa
     4801 gatctgaaag aacgcactag agtcacaaat actgaacagt tggtcttcat tgccgcattg
     4861 aatatcagct atgagttagc gcaagaaaaa gcgaagactc gtgactacgc ggcaagtatg
     4921 gaacagcgta ttcggatgct gcagcagacc atagaacaag cgttacttga acaaggtcgc
     4981 atcaccgaaa aaactaacca aaactttgaa tgacactttt cggtttactg tggtagagta
     5041 accgtgaaga caaaatttct ctgagatgtt cgcaagcggg ccagtcccct gagccgatat
     5101 ttcataccac aagaatgtgg cgctccgcgg ttggtgagca tgctcggtcc gtccgagaag
     5161 ccttaaaact gcgacgacac attcaccttg aaccaagggt tcaagggtta cagcctgcgg
     5221 cggcatctcg gagattccct tcttatctgg caccagccat gacgcaacta ccagaactcc
     5281 cactgacatt atcccgacaa gaaatccgca aaatgattcg gcaacgtcgt cgtgcgttaa
     5341 cgccggaaca acagcaggaa atgggtcaac aagccgctac ccggatgatg acttatcccc
     5401 cggtggtgat ggcgcatacg gtcgctgtat tcctctcttt tgatggtgaa ctcgacaccc
     5461 agccgctaat agaacaactc tggcgcgccg gtaagcgcgt atatcttcca gttttgcatc
     5521 cctttagtgc cggtaatttg ctgttcctga attaccatcc gcaaagcgaa ctggtgatga
     5581 acaggttgaa gatccatgag ccaaaactgg atgtgcgtga cgtgctaccc ctttcccgat
     5641 tagacgtgct gatcacaccg ctggtcgcct ttgatgagta cggtcagcgc ctgggaatgg
     5701 gcggtggttt ttatgatcgg accttacaaa actggcagca ctataaaacg caaccggtgg
     5761 gttatgcgca tgattgtcag ttggtggaaa aactccccgt tgaagagtgg gatatccctc
     5821 ttcctgcggt ggttacaccg tcgaaagtct gggagtggta agggcgatac acctgcatcg
     5881 ccctgattga catcgttgat tctttaacct aatttagtga gtaagggtaa gggaggattg
     5941 ctcctcccct gtgactgact gttaataagc tgggaaactt atgagtaaca gtacaatcag
     6001 tatgatggca agtcgcatca taacccttct ccttcaagcc ctcgcttcgg tgagggcttt
     6061 accgttacag ccccatgctg cccagctatc gtaagctctc tttaaataac cacaacgcat
     6121 tgatctacct ttaaatcttt tcttcgagcg tgctcgaaaa acccatgcaa aacagagata
     6181 atttttggga aaaaggatct attacggcag taaacgtggg cgatgaagtg gctatcaaaa
     6241 agttcggttc agggcgatgc accccgcatc gccctgattg acatcgttga ttctttgacc
     6301 taatttagtg agtaagggta agagaggatg actcctcccc tgagactgac tgttaataag
     6361 ctccgaaact tatcagtaac agtacaatca gtatgatgac aagtcgcatc ataacccttc
     6421 tcctttaagc cctcgcttcg gtgagggctt taccgttaca gccccatgct gtcccgccat
     6481 cgtaaatccc cattaaataa acacaacgca ttgatctgag tttgatttat tttctggagc
     6541 agactcgcaa agtagaatgc gcaacgcggc aacggtgtgg agaagggata aaaaaacggg
     6601 caagtcagtg acctgcccgt taattttcag agaaggggaa ttagtacagc agacgggcgc
     6661 gaatggtacc cggaatagct ttcattgcct gcagcgcttt ttcggcaacg tcttcgtcag
     6721 cttcaatatc aataactaca taacccatct gggcggaagt ttgtagatat tgcgcggcga
     6781 tgttgacgcc ctgctcggcg aagattttgt tcagcgcagt tagcacgccc ggacggtttt
     6841 cgtggatgtg catcagacga cgaccaccat gcagtggcag cgagacttcc gggaagttca
     6901 ccgcagagag cgttgagcca ttgtcagaat acttgatcaa tttacccgca acttccaggc
     6961 cgatattctc ctgcgcttcc tgagtcgaac cgccaatgtg tggcgtcaga aggacgttgt
     7021 cgaattcaca cagtggagag gtaaatggat cgctattggt cgccggttcc gtcgggaata
     7081 cgtcgattgc cgcccccgcc agatgtttgc tcgccagcgc atcacacagc gccggaatat
     7141 ccaccacagt accgcgcgag gcattaatca gcagcgagcc gggcttcatt agcgaaatct
     7201 ctttcgcgcc catcatattt ttggtggacg gattctctgg tacatgcaga ctcaccacat
     7261 cgctcatatt cagcaggtca gaaagatgct gtacctgagt ggcgttgccc agcggcagtt
     7321 tgttttcaat atcataaaag taaacataca ttcccagcga ttcagccaga atgcccaatt
     7381 gcgtaccaat atgaccgtag ccgatgatac ccagcttttt gccgcgcgct tcaaaagaac
     7441 ccgccgccag tttgttccac acgccacggt gcgctttagc attggcttcc ggcacgccgc
     7501 gcaatagcag cagcagttcg ccaatcacca gctccgcaac agagcgcgta tttgagaacg
     7561 gtgcgttaaa taccgggatc ccgcgctttg ccgccgcatc cagatcaacc tggtttgttc
     7621 cgatacagaa acagccaata gcgaccagtt tttctgcggc gttgatcacg tcttcagtca
     7681 gatgggtacg ggatcgcagg ccgatgaagt gggcatcgcg gatggattct tttaattgtt
     7741 catcatccag cgcgcctttg tgaaattcga tgttggtgta accagctgca cgaaggcttt
     7801 ccagcgcctt ttggtgcacg ccttctacca gcagaaactt aatcttgtct ttctccagcg
     7861 atacctttgc catttaccca atcctgtctt ttgaaatgtt gtgtgcggat ttgcatccgc
     7921 ctttcaacat atcaaaaaat aatattgcgg caatatgaac gtttgcgtcg cgatgttgag
     7981 gaaatatcac gcaaaggtat ttcttggttg aatatgctgg tttagaggat ttatttagag
     8041 caatcgacaa tagccaagta aaaacgtgac acatgtcacc aaatttaatg aagagaattt
     8101 ttttaacggg ggaggttccc ccgtcagatc atttcacaat ggttttgaca ccgtcaggtg
     8161 tgccaatcag cgcaacgtcc gcgccacggt tagcaaacaa gccaacagtc accacgccag
     8221 gaatcgcatt tatggcgttt tccatcgcta tcgggtcaag gatttccatg ccgtggacgt
     8281 cgaggatcac gttgccgtta tcggtcacca cgccctgacg gtattccgga cgaccgccca
     8341 gtttcaccag ctggcgcgcc actgcactac gtgccatcgg gataacttct actggcagcg
     8401 ggaatttgcc cagaatatca acctgcttgg aagcgtcagc aatacagata aatttttccg
     8461 caaccgaagc aatgattttt tcacgggtca gcgccgcgcc gccgcctttg atcatttgca
     8521 tgtggccgtt gatttcatct gcgccatcaa cgtagatgcc aaggctgtcg acttcgttga
     8581 gatcaaaaac gtgaatgccg aggcttttca gtttttcagt ggaagcatct gaactggaaa
     8641 cggccccttc aatctggcct ttcattgtac cgagcgcgtc aataaagtgt gcggcggtgg
     8701 aacctgtacc tacaccaaca atggtgccgg gctgaacata ctgaagtgcc gcccatccta
     8761 ctgctttttt caattcatcc tgcgtcatga tcgtttcgcc tgtggtatga aatttcacac
     8821 gcattatata caaaaaaagc gattcagacc ccgttggcaa gccgcgtggt taactcatcc
     8881 ataaaatatc gcgcaatggc gggcatcccc tttcgccccg caaataaagc atacaacggt
     8941 ctgggtatgc cgctccacgg tgcgaacagg cgcaccagtt ccccccgttc gcaagcccct
     9001 gtttacaggc aaattgaggc aataacgcca ccccattcaa cacaaccagg gcacgaactc
     9061 gctgagcgaa atgcattagc gcaaatccct gatcttttaa gctgcgcgtt taaccacgga
     9121 tacaaatgtt acccgccgac ggcttcggta tatgcaacct gacacaaaat tgtgtcatag
     9181 tgcaggaaaa agcatttacc aggagcagac aacagcaatg aaacgcccgg actacagaac
     9241 attacaggca ctggatgcgg tgatacgtga acgaggattt gagcgcgcgg cacaaaagct
     9301 gtgcattaca caatcagccg tctcacagcg cattaagcaa ctggaaaata tgttcgggca
     9361 gccgctgttg gtgcgtaccg taccgccgcg cccgacggaa caagggcaaa aactgctggc
     9421 actgctgcgc caggtggagt tgctggaaga agagtggctg ggcgatgaac aaaccggttc
     9481 gactccgctg ctgctttcac tggcggtcaa cgccgacagt ctggcgacgt ggttgcttcc
     9541 ggcactggct cctgtgttgg ctgattcgcc tatccgcctc aacttgcagg tagaagatga
     9601 aacccgtact caggaacgtc tgcgccgcgg cgaagtggtc ggcgcggtga gtattcaaca
     9661 tcaggcgctg ccgagttgtc ttgtcgataa acttggtgcg ctcgactatc tgttcgtcag
     9721 ctcaaaaccc tttgccgaaa aatatttccc taacggcgta acgcgttcgg cattactgaa
     9781 agcacctgtg gtcgcctttg accatcttga cgatatgcac caggcctttt tgcagcaaaa
     9841 cttcgatctg cctccaggca gcgtgccctg ccatatcgtt aattcttcag aagcgttcgt
     9901 gcaacttgct cgccagggca ccacctgctg tatgatcccg cacctgcaaa tcgagaaaga
     9961 actagccagc ggtgaactga ttgacttaac gccagggcta tttcaacgcc ggatgctcta
    10021 ctggcaccgc tttgctcctg aaagccgcat gatgcgtaaa gtcactgatg cgttgctcga
    10081 ttatggtcac aaagtccttc gtcaggatta atccatcaaa taatgcctga tagcacatat
    10141 caggcgttat cctcacttct ttttgtattc cttgaatcac atcacaaaat agacaaatct
    10201 caggcggcaa aaaacgacgt ctgaatgcat ttttttgctg gcgacaaacc cacgtaaaaa
    10261 gctcaccgta agcgcaaata ccctcatttt gattgcgttt tacggagcaa ataatgtcta
    10321 acgagcagga gtggcaacag cttgccaaca aggaattgag ccgtcgggag aaaactgtcg
    10381 actcgctggt tcagcaaacc gcggaaggga tcgccatcaa gcagctgtat accgaagccg
    10441 atctcgataa tctggaggtg acaggtaccc ttcctggttt gccgccctac gttcgtggcc
    10501 cgcgtgccac tatgtatacc gcccaaccgt ggaccatccg tcagtatgct ggtttttcaa
    10561 cagcaaaaga gtccaacgct ttttatcgcc gtaacctggc cgccgggcaa aaaggtcttt
    10621 ccgttgcgtt tgaccttgcc acccaccgtg gctacgactc cgataaccca cgcgtggcgg
    10681 gtgacgtcgg caaagcgggc gtcgctatcg acaccgtgga agatatgaaa gtcctgttcg
    10741 accagatccc gctggataaa atgtcggttt cgatgaccat gaatggcgca gtgctaccag
    10801 tactggcgtt ttatatcgtc gccgcagaag agcaaggtgt tacacctgat aaactgaccg
    10861 gcaccattca aaacgatatt ctcaaagagt acctctgccg caacacctat atttacccac
    10921 caaaaccgtc aatgcgcatt atcgccgaca tcatcgcctg gtgttccggc aacatgccgc
    10981 gatttaatac catcagtatc agcggttacc acatggggga agcgggtgcc aactgcgtgc
    11041 agcaggtagc atttacgctc gccgatggga ttgagtacat caaagcagca atctctgccg
    11101 gactgaaaat tgatgacttc gctcctcgcc tgtcgttctt cttcggtatc ggcatggatc
    11161 tgtttatgaa cgtcgccatg ttgcgtgcgg cacgttattt atggagcgaa gcggtcagtg
    11221 gatttggcgc acaggatccg aaatcactgg cgctgcgtac ccactgccag acctcaggct
    11281 ggagcctgac tgaacaggat ccgtataaca acgttatccg caccaccatt gaagcactgg
    11341 ctgcgacgct gggcggtact cagtcactgc ataccaacgc ctttgacgaa gcgcttggtt
    11401 tgcctaccga tttctcagca cgcattgccc gcaacaccca gatcatcatc caggaagaat
    11461 cagaactctg ccgcaccgtc gatccactgg ccggatccta ttacattgag tcgctgaccg
    11521 atcaaatcgt caaacaagcc agagctatta tccaacagat cgacgaagcc ggtggcatgg
    11581 cgaaagcgat cgaagcaggt ctgccaaaac gaatgatcga agaggcctca gcgcgcgaac
    11641 agtcgctgat cgaccaaggc aagcgtgtca tcgttggtgt caacaagtac aaactggatc
    11701 acgaagacga aaccgatgta cttgagatcg acaacgtgat ggtgcgtaac gagcaaattg
    11761 cttcgctgga acgcattcgc gccacccgtg atgatgccgc cgtaaccgcc gcgttgaacg
    11821 ccctgactca cgccgcacag cataacgaaa acctgctggc tgccgctgtt aatgccgctc
    11881 gcgttcgcgc caccctgggt gaaatttccg atgcgctgga agtcgctttc gaccgttatc
    11941 tggtgccaag ccagtgtgtt accggcgtga ttgcgcaaag ctatcatcag tctgagaaat
    12001 cggcctccga gttcgatgcc attgttgcgc aaacggagca gttccttgcc gacaatggtc
    12061 gtcgcccgcg cattctgatc gctaagatgg gccaggatgg acacgatcgc ggcgcgaaag
    12121 tgatcgccag cgcctattcc gatctcggtt tcgacgtaga tttaagcccg atgttctcta
    12181 cacctgaaga gatcgcccgc ctggccgtag aaaacgacgt tcacgtagtg ggcgcatcct
    12241 cactggctgc cgatcataaa acgctgatcc cggaactggt cgaagcgctg aaaaaatggg
    12301 gacgcgaaga tatctgcgtg gtcgcgggtg gcgtcattcc gccgcaggat tacgccttcc
    12361 tgcaagagcg cggcgtggcg gcgatttatg gtccaggtac acctatgctc gacagtgtgc
    12421 gcgacgtact gaatctgata agccagcatc atgattaatg aagccacgct ggcagaaagt
    12481 attcgccgct tacgtcaggg tgagcgtgcc acgctcgccc aggccatgac gctggtggaa
    12541 agccgtcacc cacgtcatca ggcgctaagt acacagctgc ttgacgccat tatgccgttc
    12601 tgtggtaacg ccctgcgact gggcgttacc ggcacacccg gcgcggggaa aagtaccttt
    12661 cttgaagcct ttggcatgtt gttgattcgt gagggattaa aggttgctgt tattgcggtc
    12721 gatcccagca gcccggtcac tggcggtagc attctcgggg ataaaacccg catgaatgac
    12781 ctggcgcgtg ccgaggcggc gtttattcgc ccggtaccct cctccggtca tctgggcggt
    12841 gccagtcagc gagcacggga attaatgctg ttatgcgaag cggcgggtta tgacgtagtg
    12901 attgtcgaaa cggttggcgt cgggcagtcg gaaacggaag tcgcccgcat ggtggactgt
    12961 tttatctcgt tgcaaattgc cggtggcggc gatgatctgc agggcattaa aaaagggctg
    13021 atggaagtgg ctgatctgct cgttatcaac aaagacgatg gcgataacca taccaatgtc
    13081 gccattgccc ggcatatgta cgagagtgcc ctgcatattc tgcgacgtaa atacgacgaa
    13141 tggcagccac gggttctgac ttgtagcgca ctggaaaaac gtggaatcga tgagatctgg
    13201 cacgccatca tcgacttcaa aaccgcgcta actgccagtg gtcgtttaca acaagtgcgg
    13261 caacaacaat cggtggaatg gctgcgtaag cagaccgaag aagaagtact gaatcacctg
    13321 ttcgcgaatg aagatttcga tcgctattac cgccagacgc ttttagcggt caaaaacaat
    13381 acgctctcac cgcgcaccgg cctgcggcag ctcagtgaat ttatccagac gcaatatttt
    13441 gattaaagga atttttatgt cttatcagta tgtgaacgtt gccactatca acaaagtggc
    13501 ggtcattgag tttaactatg gacgaaaact taatgcctta agtaaagtct ttattgatga
    13561 tcttatgcag gcgttaagcg atctcaaccg gccggaaatt cgctgtatca ttttgcgggc
    13621 gccaagtgga tccaaagtct tctccgcagg tcacgatatt cacgaactgc cgtctggcgg
    13681 tcgcgatccg ctctcctatg atgatccatt gcgtcaaatc acccgcatga tccaaaaatt
    13741 cccgaaaccg atcatttcga tggtggaagg tagtgtttgg ggtggcgcat ttgaaatgat
    13801 catgagttcc gatctgatca tcgccgccag tacctcaacc ttctcaatga tgcctgtaaa
    13861 cctcggcgtc ccgtataacc tggtcggcat tcacaacctg acccgcgacg cgggcttcca
    13921 cattgtcaaa gagctgattt ttaccgcttc gccaatcacc gcccagcgcg cgttggctgt
    13981 cggcatcctc aaccatgttg tggaagtgga agaactggaa gatttcacct tacaaatggc
    14041 gcaccacatc tctgagaaag cgccgttagc cattgctgtt atcaaagaag agctgcgtgt
    14101 actgggcgaa gcgcacacca tgaactccga tgaatttgaa cgtattcagg ggatgcgccg
    14161 cgcggtttat gacagcgaag attaccagga agggatgaac gctttcctcg aaaaacgtaa
    14221 acctaatttc gttggtcatt aatccctgcg aacgaaggag taaaaatgga aactcagtgg
    14281 acaaggatga ccgccgatga agcggcagaa attatccagc ataacgacat ggtggcattt
    14341 agcggcttta ccccagcggg ttcgccgaaa gccttaccga ccgcgattgc ccgcagagct
    14401 aacgaacagc atgaggccaa aaagccgtat caaattcgcc tgctgacggg ggcgtcaatc
    14461 agcgccgccg ctgacgatgt actttctgac gccgatgctg tttcctggcg tgcgccatat
    14521 caaacatcgt ccggtttacg taaaaagatc aatcagggcg cggtgagttt cgttgacctg
    14581 catttgagcg aagtggcgca aatggtcaat tacggtttct tcggcgacat tgatgttgcc
    14641 gtcatcgaag catcggcact ggcaccggat ggtcgagtct ggttaaccag cgggatcggt
    14701 aatgcgccga catggctgct gcgggcgaag aaagtgatca ttgaacttaa tcactatcac
    14761 gatccgcgcg ttgcagaact ggcggatatt gtgattcctg gcgcaccacc gcggcgcaat
    14821 agcgtgtcga tcttccatgc aatggatcgc gtcggtaccc gctatgtgca aatcgatccg
    14881 aaaaagattg tcgccgtcgt ggaaaccaac ttgcccgacg ccggtaatat gctggataag
    14941 caaaatccca tgtgccagca gattgccgat aacgtggtca cgttcttatt gcaggaaatg
    15001 gcgcatgggc gtattccgcc ggaatttctg ccgctgcaaa gtggcgtggg caatatcaat
    15061 aatgcggtaa tggcgcgtct gggggaaaac ccggaaattc ctccgttcat gatgtattcg
    15121 gaagtgctac aggaatcggt ggtgcattta ctggaaaccg gcaaaatcag cggggccagc
    15181 gcctccagcc tgacaatctc ggccgattcc ctgcgcaaga tttacgacaa tatggattac
    15241 tttgccagcc gcattgtgtt gcgtccgcag gagatttcca ataacccgga aatcatccgt
    15301 cgtctgggcg tcatcgctct gaacgtcggc ctggagtttg atatttacgg gcatgcaaat
    15361 tcaacgcacg tagccggggt cgatctgatg aacggcatcg gcggcagcgg tgattttgaa
    15421 cgcaacgcgt atctgtcgat ctttatggct ccgtcgattg ctaaagaagg caagatctca
    15481 accgtcgtgc caatgtgcag ccatgttgat cacagcgaac acagcgtcaa agtgatcatc
    15541 accgaacaag ggatcgccga tctgcgcggt ctttccccgc ttcaacgcgc ccgcactatc
    15601 attgataatt gtgcacatcc tatgtatcgg gattatctgc atcgctatct ggaaaatgcg
    15661 cccggcggac atattcacca cgatcttagc cacgtcttcg acttacaccg taatttaatt
    15721 gcaaccggct cgatgctggg ttaatggttt gtcccgtcac gatcttcagc aatatgctga
    15781 agcatcgtgg cggtataacg atgatttctg attgaataaa gatattcctg catatacatc
    15841 ggcctgtgcg gcgcatcaaa atatttcagc gttgccggat tcaccagtcg ccaggcaaaa
    15901 tgcgggatca ccgtcagaaa atgtccgcgc tccacggcgc tgattttggc cataaaactg
    15961 tagggacgat aaataatggt gggattgatc ccacaaggtc gcatattcgc atcaatcatt
    16021 gcttcgaagt tagcccggtt ttgaaagcgc atttgtagcc agggcagctc ctgaagcagc
    16081 tcttgctctt gacgctcttc ataacggcga gaaacgagaa agcccaatcg cagcggtggt
    16141 aattcactga tagtcaggtt ttccagttcc tgaactcgtg cagatacatg ctgtggggag
    16201 ataataaaat ctaactgacg gtcaaaaaga ttatcgataa cgccgttttc gctgaactca
    16261 actggctggg cggtcacgcc ttcatactta tctccgagac taatcaactg atcgaaaata
    16321 attgtcggat aagtattatc tatgccaatc acaatt
//
LOCUS       DAZOYL010000067        16339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZOYL010000067 DAZOYL010000000
VERSION     DAZOYL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.067"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            210..710
                     /gene="tssB"
                     /locus_tag="ACHUOY_001585"
     CDS             210..710
                     /gene="tssB"
                     /locus_tag="ACHUOY_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR0682394.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            745..969
                     /locus_tag="ACHUOY_001586"
     CDS             745..969
                     /locus_tag="ACHUOY_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0682395.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1020..2495
                     /gene="tssC"
                     /locus_tag="ACHUOY_001587"
                     /pseudo
     CDS             1020..2495
                     /gene="tssC"
                     /locus_tag="ACHUOY_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2502..2915
                     /gene="tssE"
                     /locus_tag="ACHUOY_001588"
     CDS             2502..2915
                     /gene="tssE"
                     /locus_tag="ACHUOY_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR0682396.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2919..4769
                     /gene="tssF"
                     /locus_tag="ACHUOY_001589"
     CDS             2919..4769
                     /gene="tssF"
                     /locus_tag="ACHUOY_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR0682397.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4733..5815
                     /gene="tssG"
                     /locus_tag="ACHUOY_001590"
     CDS             4733..5815
                     /gene="tssG"
                     /locus_tag="ACHUOY_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR0682398.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5840..7120
                     /gene="tagH"
                     /locus_tag="ACHUOY_001591"
     CDS             5840..7120
                     /gene="tagH"
                     /locus_tag="ACHUOY_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR0682399.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7117..7641
                     /gene="tssJ"
                     /locus_tag="ACHUOY_001592"
     CDS             7117..7641
                     /gene="tssJ"
                     /locus_tag="ACHUOY_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR0682400.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7644..8973
                     /gene="tssK"
                     /locus_tag="ACHUOY_001593"
                     /pseudo
     CDS             7644..8973
                     /gene="tssK"
                     /locus_tag="ACHUOY_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            8978..9739
                     /gene="icmH"
                     /locus_tag="ACHUOY_001594"
     CDS             8978..9739
                     /gene="icmH"
                     /locus_tag="ACHUOY_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR0682401.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9748..12537
                     /gene="tssH"
                     /locus_tag="ACHUOY_001595"
                     /gene_synonym="clpV"
                     /pseudo
     CDS             9748..12537
                     /gene="tssH"
                     /locus_tag="ACHUOY_001595"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            12534..13277
                     /gene="vasI"
                     /locus_tag="ACHUOY_001596"
     CDS             12534..13277
                     /gene="vasI"
                     /locus_tag="ACHUOY_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR0682402.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13282..14694
                     /gene="tssA"
                     /locus_tag="ACHUOY_001597"
     CDS             13282..14694
                     /gene="tssA"
                     /locus_tag="ACHUOY_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR0682403.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLLILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14713..>16339
                     /gene="tssM"
                     /locus_tag="ACHUOY_001598"
     CDS             14713..>16339
                     /gene="tssM"
                     /locus_tag="ACHUOY_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFR0682404.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPY"
BASE COUNT         3737 a         4531 c         4543 g         3528 t
ORIGIN      
        1 tcacacaatc tatttaacgc ttgcagaata cccctcttta cgatcgataa aaagtccttt
       61 tcttatttat tttgaagcgt aaaatgcggg gtgatgaagg agtggtgatt ttattttcac
      121 cgccattagg gcggcattgc cgatattttt ttgcggtaca aatatgccgt gttcatggcc
      181 tgtacgagat aacatgacgg atcactttta tgagcaaaaa atttgaaggc agcgtcgcgc
      241 cccgcgagcg tattaatata tcgtatgtcc caaaaacaga tggccagacg gctgaagtgg
      301 aacttccgct taacatgctc gttgtcggcg ataccggaaa tacgcaggaa acatccccac
      361 tggatgaacg tcaggcagtt tccgttaaca agcacaattt cggtgcagtg atggctgaag
      421 cagccattgg cctcaatttt actgtccccg ccacgctgaa gggaagcaca acagacgatg
      481 aactgaatgt ggcgctgaat atcaaatcac tggatgattt ttcaccagat agcgtggccc
      541 ggcaggttcc ggaagtgaat aaattgctgg aactgcgtga agcactgact gcactgaagg
      601 ggccgatggg taaccttccg gcattccgta cccagctcca ggcattgctg gaaaatgaag
      661 agtcccgcga gcagttgctc aaagaaatcg gtctggtgag caataaataa ccaccactat
      721 ttcattaaac agggttatcc aggcatgacg gcacacaaaa atatctccgg gacataccac
      781 ctctctgttg cagacattct gcaggtggtt taccaggtat gtttcagccc gtccgttgaa
      841 atcaaccagg atggtgtggc tgcgctgatt acaacactgg atcgccgtat cagtgatcta
      901 ttggatgaga ttatccattt ttgtgagttt cagcagagcg cgtcgcactg gcagcgcgta
      961 cttcactgat ggcgaaaagc cggtttcaga aacacagcta aaacagaagg attttcagta
     1021 tgtctttaca ggaagaggaa ctcgtttcca gtcatgccgg gcagcctgag caggcatcgt
     1081 cgctgcttga tcaaatcatg gcacagaccc gtattcagcc cggttcggaa ggttatgacg
     1141 ttgccctcca gggtgtgaca gcctttattg ccagcatcct gcaatccact gcatctgccg
     1201 aaccggtcaa taaactggcc gtggacagca tgattgcaga tattgatgaa cgcatcagcc
     1261 gccagatgga tgtcatcatc catgcgcctg cctttcagca ggtcgagtcg ttctggcgct
     1321 cactgaaaac catggtggat cgcgttgatt tccgtgaaaa catcaaggtc aacgtgcttc
     1381 atgtcaccaa gcaggaactt ctggaagact ttgagtttgc gccagaaatt attcagtccg
     1441 gattttataa gcacgtttat tcgtccggtt tcggacagtt tggcggtaaa cccatcgctg
     1501 ccgtgctggg cgcttatgag ttcaaaaata ccgcgcctga catgaaactg ctgcaatacg
     1561 tcagtgccgt gggcgcaatg gcgcacgcac cgttcctgtc ctccgtttcc ccggagttta
     1621 tggggctgaa ctcgtggacc gaactgccta atatcaaaga tctttatgcc atctttgaag
     1681 gtccggccta caccaaatgg cgcgccctgc gtgactcgga agattcgcgc tatttagggc
     1741 ttacggcacc ccgcttcctg cttcgccagc cctattcacc aacagataac cctgttaaga
     1801 actttaacta ttacgaagat gtcagccaga accacgaaga ttatctgtgg ggtaatacgg
     1861 cctggatgct ggcatgcaat atcgcagaca gttttgccaa ataccgctga tgtccaaaca
     1921 ttatcggtcc gcaaagcggc ggcgcagtga aagatctgcc gatgcatctg ttcgaaacga
     1981 tggggcagat tcaggccaaa atcccaaccg aggtgctggt caccgatcgc cgcgaatttg
     2041 aactggctga agagggtttt atcaccctta ccatgcgtaa agactctgat aatgcagcct
     2101 ttttctctgc aaactcggta caaaaaccga agcactttcc gggaaaagat gcagaaacca
     2161 actataagct gggtacgcag cttccttatc tcttcatcat caatcggtta gcgcactaca
     2221 tcaaggtgtt gcagcgtgaa caactggggt catggaaaga aaggagcgac ttagaaagag
     2281 agcttaatac ctggatccgt cagtatgttg ccgaccagga aaacccgcct gctgacgtgc
     2341 gcagccgcaa acctctgcgc gctgccaaag ttgaggttat ggatgtagaa ggcgaacccg
     2401 gctggtacca ggttgcgcta agcgtgagac ctcattttaa atttatgggg gcaaattttg
     2461 agctttccct ggttggccgg ttagacaggg agtaacacac catgataccg gatcgggaaa
     2521 gaaccacagg ttctcttttt gagcgtatgg aagcctcttc ggccagaaac cggcagggag
     2581 gcagtattca ttcgctcagg caatccattc gccagaacct acgaaacatt cttaataccc
     2641 ggtccggtag ttgccggggt gcgcctgagc tgggcattga tgaaccggaa ggggctgaaa
     2701 acttccgcga atccatgagt cgggccatcg agcaatgtat agaacgctat gaaccccgga
     2761 tctcacacgc tgaagtgcag gcagtggttt catccgcctc cagcccgctg gatatgacgt
     2821 ttcatattac agcctgggtg acatttaacg aaacgcatga ggtgcttgaa tttgatatgg
     2881 caccgaatgg cagccagcat taccgggtgg actgacaaat ggaatttgaa gaacgctatt
     2941 tccgggaaga actcgattac ctgcgccagc ttagcaagct gctggcaacg gaaaaacccc
     3001 atctggcccg cttcctggcc gaaaaagatg cagatccgga tattgaacgc ctgctggaag
     3061 gggtggcttt tcttaccggc aatctccgcc agaaaattga ggatgaattc ccagaactga
     3121 cgcacgggct tattaagatg ctatggccta attacctgcg tccggttccg gcaatgaccc
     3181 ttattgaata tacgccggat atggataagt cttctgtacc ggtgttaatc ccccgtaatg
     3241 agcagtttac aaccaacgcc ggggaaatca gagttgatga agtgctgccc tctgatgcta
     3301 aaaaggagga gccgcctccc tgtaccttca ccctctgccg ggatatctgg ctgctgcccg
     3361 ttcgcctgga gcagattgaa aaccgcagta cgacccgtaa tggtgttatc aacatcacct
     3421 tttcggtcgc accgggaacg gacttccgca cgctggatct gaacaaactt cgcttctggc
     3481 tcggcaatga cgacaactat acccgtgacc agctttattt atggttctgc gaatacttgc
     3541 agggtgccga cctgactgtg ggtgaacagc atattcgcct gcctgagttt atgctaaaag
     3601 ctgtcggttt tgagccgcag gatgccatgc tgccctggcc gaaaaacgtc cacagcggct
     3661 accggatcct tcaggagtat ttctgttacc ccgatgcgtt tctctttttt gatctttgtg
     3721 gttgtccggc tttgcctgac ggattgcagg cggaattctt taccctgcaa ctgcgttttt
     3781 cgcgcccttt gcccgtggac atccggctgc gccgcgattc cctgcgcctg tattgcgcac
     3841 ctgccattaa tttatttatc caccatgcag aagccatcac gctggacaac cggcgggcag
     3901 actatccgct ggttcccagc cgccattacc cacaacatta cgatgtattt tccgttaaca
     3961 gtgtggtgag ccaggtccag gatatgttca ggaaaaaaga tctggggcgt cctgtttcga
     4021 cgcaggccgc gcgccagtgg ccagcctttg aaagtttcag ccatcagata gaatacagcc
     4081 ggaagcggga agtggtgtac tggcatcacc ggaccaaaac atccctgttc catcgcggct
     4141 ttgatcatac ccttgccttt atacatgctg atggcagtta tccgtcagac gaatctctgc
     4201 tcagtaatga agtggtttcg gtatcgctga cctgtaccaa ccgtgagctt ccgtcacaaa
     4261 ttcgttccgg cgatatcacc ggcacaaccg gaaaaaatgc ggctgttgct tcatttcgca
     4321 acattacccg cccgacgcaa ccactctggc cggtcattga tggcagcctg cactggcccc
     4381 tactctccgc catgaacctg aattatctgt cattactgga tacggacgcg ctgaagcagg
     4441 tcatcgccaa ctttgatcgc cacgcaatcc atcatccgca gacggcacgg ctgtcacaac
     4501 aaaagctgga tgccattgag cgtctggaga cccgccccgt tgatcgcctg tttacgggta
     4561 ttcccgtccg ggggctggct tccacgctgt atctgcaccc ggagccgttt gtctgtgaag
     4621 gggaaatgta tctgctcggt acggtgcttt cgcattttct gtcgctgtac gccagcgtta
     4681 actcattcca catgctgacc gttgtgaaca cagaaagcca ggagacatgg aaatggacgg
     4741 aaagaatcgg gcagcatcct cttatctgag tccgggaaat cccccggcag ataaagagca
     4801 gaatgatccg ctggcacagg ttttccataa tgcctgctct tacaattttt ttgcgatggc
     4861 ggagctgttg caccgcctgg caaagggtga aaaggggacg ccagaattat ccctgcgcga
     4921 cgatccggca caggaaaccc tgcgtttcag cgccgatgcc agccttgcct tcccgtgcag
     4981 tgatatcagt gcgctgaaac gggatacgtc aggggcattc aggatgacca ccacctttat
     5041 ggggttacag gggagccagt cgcctctccc cggttattac ctcgctcacc tggcctggaa
     5101 agcggtacat gaacaaagtc cggtaggtga ttttctggat atgttcagcc accgcctgac
     5161 ccagtttgtc tggcatatct ggcgtaagta ccgctatcac atcagttttc gtaacggcgg
     5221 tgtggatgcg ttctcgcagc gcatgtactc ccttgtcggg ctgggccacc gccagcttcg
     5281 cgataagctg gcgattaatc acagcaaaat gctggcgtat tccgggatcc tggcaaatcc
     5341 gggacgatcc ccggaaatta tctgtggcct ggtgtcccac tgctttgatt tgtcagaagt
     5401 gacactgcaa aactggcaac gtcgcaaggt ggatattgaa ccggatcagc aaaatagtct
     5461 ggggagctat tctcttaaaa atggcgaaaa actggcggga agatcggtac tgggcaactt
     5521 tgttctgggt acacgagtgc ccgatctcag cggaaaattc cagctcagta ttaccagcct
     5581 gacccgaaaa cagttcctct cttttctgcc gtccggcgaa aacttcctgc cactgacgat
     5641 gttcgtgtcc ttcatcctgc gcgatcagct tgcctgggac ttacatctgg ggctggcgcc
     5701 ggagcaggtg ggcgcaatgc gcctgggcga taacaaaagt gcgctgctgg gctggaccag
     5761 cttcctcggc acaccggaag aacgaccctc agtcaccatc agggtacggt cgtaaattat
     5821 gataatcacg gagcagaata tgccggaaga gaaactacag acgctttcat tgcaggtcat
     5881 taacggcagt gagctggaaa gcgggcgggc ggcgcgctgt ctgttcacac agcagggaaa
     5941 tgtcggccat ggccccgaat gccactggtc ggtacaggat cgtcagcaga gcattccggc
     6001 ccaggctttt accgttatcc tgcacgatgg cacattttgt ctgcgcccgc agacggcaca
     6061 actgtggctg aatcaggcaa aagtcacagc aacatcagac ctaatacagt tgcgccaggg
     6121 cgatgagatc cagatcggac ggctgatggt gagggttcat ctgaaccggg gagatattcc
     6181 ccattacgat gaggaaatgg ccactcccga aaccatcgtt accaatcgcg atatgctcac
     6241 ggataccctg ctatcaacgg agggtgcgcc acactatccg ggaatgactc accggcacca
     6301 gcttgcagac accgtggtaa acggtttttc tgccgatcca ctccaggctc ttcagtccga
     6361 aagcctgatt accacgggcg atccgctttc aggcattgcg gctgtccggc catcggcacc
     6421 gctgtccgat ccggcaagta atggggggat caatactccg tttatggatc tgccgcccat
     6481 ttatgccagc cccggcgatc gtaatgacga cgtttctgcg gcagaaatgg cacaacgcca
     6541 cgttgcggtc acccccttac tgcgcggtct tggcggctcg cttaccatga gcaattccga
     6601 cgatgcggat gattttctgg aggaggccgg acgaacgtta caggccgcaa taaaaggtct
     6661 gctcgatttg cagcagcagc gtaacagcct ctcagacaaa catttgcgcc cgctggaaga
     6721 taacccgctg cgcctgaaca tggattacgc caccgcgctc gacgtaatgt ttgccgaagg
     6781 taaaagcccg gtacatctgg cggctcccgc cgccgtcagt gaaagcctgc gcaatgtccg
     6841 ccaccacgaa gaagctaacc gggcagcgat tgtggagtcg cttcgtgtcc tgctggatgc
     6901 tttctcacca caaaatctgc tgcgccgctt tgtgcagtac cgccgcagcc atgaactgcg
     6961 ccagccgctg gatgatgccg gagcatggca aatgtacagc cattattacg aagaactggc
     7021 ctccgatcgc cagcaggggt ttgagatgct gtttaacgag gtctacgccc aggtctatga
     7081 ccgggtgctt cgtgaaaaac agcgggagcc ggaagcatga acaggaaggc gtttctggcc
     7141 tgcgtgctga tgtgcgtcat attaaccggc tgcgaaacgg cgaaaaaaat cagtgaggtg
     7201 atcaaaaacc cggatatcca ggtcgggagc ctgaaggagc aaccttcgga aataaccgtc
     7261 accctgctga ccgaaccgga caccaatacc aatgcggaag gtgaatcagc cgccgtggat
     7321 gtgcaactgg tgtacctgac tgacgactca aaattgctgg cagccgacta tgaccagata
     7381 gccagcacgc cgctaccgga tgtgctgggt aaaaactata tcgaccacca ggatttcaac
     7441 ctgttgccgg acaccatcaa aaccctgccg ccggtaaagc tggatgagaa aacgcagttt
     7501 attggtgtcg tggcctattt ctccgatgac caggccactg aatggaaaca aatcgagacg
     7561 gtggaaggaa tcggccacca ttaccgcctg ctggtgcatg tacgtcaaag cagcatcgaa
     7621 atgaaaaaag aggatgaata attatggcaa ccacccgcaa caaggtgatg tggcaggaag
     7681 ggatgctgat gcgtccacac catttccagc agcagcagcg ttacaacgac tacctggata
     7741 accagcgttt ccgggccatg aatgatttat cctggggatt taccgaactc accctcaaca
     7801 atgaactgct ggcgcagggt aagatcatga ttgacagcgc gtcaggcaca ctgcccgacg
     7861 gcaccgtctt ttctatcccc gaccaggacg cactgcccga tccgctgcac ccgcaaaatt
     7921 ttccggacga aagaagccgc aatatctacc tcgctctgcc ggtcgccagt gatgtgagaa
     7981 atgaaatcag cgacggacgg cgaatcgggc gttaccggct gaattatgcc gatgtccggg
     8041 atttgcattc agaagaaggt gacgcgcgaa cattgacgct gggtcaactg acgccgcgca
     8101 ttatgagcgg tgcagaagat atgagcgcct atattacgct cccgctttgc cgtatcagcg
     8161 atcgccgccg atggctccct gacgttggat gacgatttta tcccctcctg ccagaatatt
     8221 caggtcagta agaaactgcg tgtttatctc aaagaggtac agggggccat tggcggacgg
     8281 gcaagcgatc tggcaaaccg tattggctct ccggcgcaga gcggcatcgc ggatgtggcg
     8341 gaatttatga tgttgcagtt acttaaccgt aaccagaccc ggtttaccca tcacgcccgt
     8401 cgatcccagc tccacccgga agatttctac cttgatcttg ccgggttgct gggtgaactg
     8461 atgaccttta ccgagccgtc gcgcctgccc tgcccgcttg atgtgtatga tcatcatgac
     8521 ctgaccaaaa tatttaaaac gctgttaccg gaagtcaaac gggcgctgca taccgtactg
     8581 tcgccaagag ccgtcaatct gccgctgcat ctgcgtgacg gtatctggca ggccgatatc
     8641 catgacacag aactgctgca atctgccacc tttgtgctgg ccgtggcggc aaacatgccg
     8701 gtcgatcaga tccagcgcca gtttatccag cagtcgaaaa tttcctcgcc ggaaaaaatc
     8761 cgcaatatgg tcagtgtgca gatccccggt attccattgc gtgcccttat ggtggctccc
     8821 cgccagcttc cttaccattc cgggttcagc tatttcgaac tcgacaagag tggccaggcc
     8881 tggacagaaa tggctgccgc cggggccgtt gcactgcatg tttccggcag tttcccggat
     8941 ctgaacatgc aactgtgggc gataagaggg taagacgatg gatgaggggt cactgtctct
     9001 gccaccattt accggacacg acgaaaaaag ccagcgcaac taccatctcg cgcttcgtgg
     9061 caacagcctg aatccgatga tcgacgcggc cacgccgcta ctcggtatgg taatgcgcct
     9121 ttcaaccatg aacagccaga cgatgccgga gcatcttttt gctcaggtgg tgacagatgt
     9181 tcaggccgtg gagcaactgc ttcaggagca gggctacgaa ccgggcgtga ttatctcgtt
     9241 tcgctacatc ctctgcacgt ttattgatga agcggcgctc ggtaacggct ggtcaaacaa
     9301 gaacgagtgg atcaagcagt cattgctggt ccatttccac aacgaagcct ggggtggcga
     9361 gaaagttttc atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc aggatctgct
     9421 ggaatttctc tggatctgtt tttctctggg atttcgcggg cgctacaaag tggcagcaca
     9481 ggatcagggg gaatttgagc agatctaccg ccgtctgtac cacgttctgc ataaactgcg
     9541 cggcgatgcc cctttcccgc tgttacacca ggacaaaaaa acccagggcg ggcgttatca
     9601 gctcatcagt cgcctgactg ttaaacatat tttttgtggt ggcgttgtcg tgctggcgtt
     9661 gttttacctg ttctatctgt tgcggctgga cagccagacg caggacatcc tgcaccagct
     9721 aaacaaatta cttcgataag gacgtttatg atccagattg atcttcccac gctggtaaaa
     9781 cggctgaacc tgttctcccg ccaggcgctg gagatggccg cctctgaatg tatgagtcag
     9841 caggcagcgg aaattaccgt cagccatgtg ctcattcaga tgctcgccat gccacgcagt
     9901 gacctgcggg ttattacccg ccagggcgat attggcatgg aagagttgcg ccaggcgctg
     9961 acggtagaga actacacaac cgcccgttct gcggacagct acccggcgtt ttccccgatg
    10021 ctggttgagt ggcttaaaga gggctagctg ctggcgtcgg ctgagatgca gcacagcgaa
    10081 ctgcgcggcg gcgtgttgct gctggccctg ctgcattcgc cgctgcgtta tataccgcct
    10141 gctgccgccc ggctgttgac cggcattaac cgtgaccgtc tgcaacagga ctttgtgcag
    10201 tggacacagg agtcggcgga atcagtcgtg ccggatgcag acggtaaagg cgcaggcaca
    10261 atgacggacg cctctgacac cctgcttgcc cgctatgcca aaaacatgac cgcagacgcc
    10321 cgtaacggca ggcttgaccc ggtactgtgc cgcgaccacg aaatcgacct gatgatcgac
    10381 attctctgcc gccgccgtaa aaacaaccca gtggtggtgg gcgaagcggg cgtgggcaaa
    10441 agcgcactga ttgaagggct ggcgctgcgc atcgtggcag gccaggtgcc ggacaagctg
    10501 aaaaacaccg atatcatgac ccttgacttg ggcgcattgc aggccggggc gtcggtgaag
    10561 ggtgaattcg aaaaacgttt caaagggctg atggcggagg tcatttcctc cccggtgccg
    10621 gtcattctgt ttatcgacga agcacatacc ctgattggcg cgggcaacca gcagggcggg
    10681 ctggatatct ccaacctgct caaaccggcg ctggcgcgcg gcgagctgaa aaccatcgcc
    10741 gccaccacct ggagcgagta caaaaaatac ttcgaaaaag atgccgccct gtcgcgccgc
    10801 ttccagttgg tgaaggtcag cgaacccaac gctgccgaag ccaccattat tctgcgcggt
    10861 ctgtcggcgg tctatgaaca gtctcacggc gtgctgattg atgatgacgc cttgcaggcc
    10921 gctgcgacat taagcgagcg ttatctctcc gggcgtcagt taccggacaa agcgattgat
    10981 gtgctggata ccgcctgcgc ccgtgtggcc atcaacctgt cgtcgccgcc gaagcaaatc
    11041 tcggcgctga ccactctgag ccaccagcag gaggcggaaa ttcgccagct tgagcgcgag
    11101 cttcgcatcg gactgcgtac cgacacatca cggatgaccg aggtgctggt gcagtatgat
    11161 gaaacgctga cggcgctgga tgaactggaa gcggcctggc accagcagca gacgctggtc
    11221 cgggagatta tcgcgctgcg ccagcagtta ctgggcgtgg cagaggacga tgcggcgccg
    11281 ttgccggacg cagataccgt ggaggatacg cagccagagt cagagtcaga gtcagagtca
    11341 gagtcagagt cagagcagga taataccggt gccgaaccgg ctgatgaggc cgacagagaa
    11401 caaccggaag agaccgctga aacagtttcc ccggtacagc ggctggccca gctcactgcc
    11461 gaactggacg ccctgcataa cgaccggttg ctggtctccc cgcacgtcga taaaaaacag
    11521 attgcggcgg tgattgccga atggaccggc gtgccgctca accgcctgtc gcagaatgaa
    11581 atgtcggtca tcaccgacct gacgaaatgg ctgggtgaca ccatcaaagg ccaggacctg
    11641 gcgattgcca gcctgcataa gcatctactg accgcacgcg ccgacctgcg tcgtccggga
    11701 cgcccgctcg gtgcgttcct gctggctggc cccagcggtg tgggtaaaac cgaaaccgtc
    11761 ctgcaactgg cagagctgct ctacggcggt cgccagtacc tgaccaccat caatatgtcc
    11821 gaattccagg agaaacacac cgtctcgcgg ctgattggtt cgcctccggg ctacgttggc
    11881 tacggtgaag gcggcgtact gaccgaagcg attcgccaga aaccctactc ggtagtactg
    11941 ctcgatgaag tggaaaaagc gcacccggat gtgctcaacc tgttctacca ggcgttcgat
    12001 aagggcgaaa tggcagacgg tgaaggccgc ctgattgact gcaaaaatat cgtcttcttc
    12061 ctgacgtcca acctcggcta ccaggtaata gtcgagcatg ccgatgaccc ggaaaccatg
    12121 caggaagtac agtatccggt gctggccgac ttcttcaaac ctgccctgct ggcgcgtatg
    12181 gaagtggtgc cgtatctgcc gctgtcgaaa gagacgctcg ccaccattat cgccggaaaa
    12241 ctggcccgtc tggataacgt gctgcgcagt cgctttggtg tagaagtggt cattgaaccg
    12301 gaagtgacgg acgaaatcat gagccgcgtc acccgcgcgg aaaacggcgc gaggatgctg
    12361 gaatcggtca tcgatggcga catgctaccg ccgctctcgc tgctgctgtt gcagaaaatg
    12421 gcggctaaca cggcgattgc ccggattcgg ttgtcggcag tggacggcgc atttacggca
    12481 gacgtggaag atgctcagaa cgacgagtcc gtcacaaagg atgaaacggt tttatgagca
    12541 ggttgcgcgt tttccggcag acaggatggt ttatcgcggg cctgatgacg ggcttacccg
    12601 ccactgccgc gcccgcagaa gcatcgtcaa tggccggtgt ggcggtcgcc gtcgccacca
    12661 cgaccccgcc ggatgcgaca gccacgttac aggccatgca gtcctgtcgg cgggagtccg
    12721 cggcgctgga gcggcttgac tgctatgacc gcctgctggc accgctgtcc ccgtcagggt
    12781 ttgacggggc gctggtcaaa gccggttttg tgggcgaggc ctggacccgc gccacagagc
    12841 aggaaaaacg ccgcgagggc aacaccacag aactgctggt gacgcaggtg ccgggggagc
    12901 gcccgacggt ggtgataacc accccggcta tcggtcatgt tccgccacgc ccggtgctga
    12961 tgttcagttg cgtggacaat atcacccgga tgcaggtggc gctgatgcac cctctggatg
    13021 tccatgatat cgccgtcact ctgaatgccg acaaccgggc attgcgcagc cactggtttg
    13081 tgcgcgagaa cggcaccctg ctggagtcca gccgggggtt atccgggatt gatgaaatta
    13141 agcaactgtt cggcgcaaaa acgctgacgg tggataccgg cgcagacaat gccgccggaa
    13201 agctgacctt taacattgac gggctggcaa gggcgattgc tcccctgcgc gatgcctgcc
    13261 actgggcggg agaataaaaa tatggcaatg gacttacgcg atccgaatgt ctggatatcg
    13321 cacctgctgg aaaacctgcc ggaagagaaa ctggcatcgg cgctgaaaga tgacaacccg
    13381 aactgggagt atatcgacgg cgaaatcgtc aagctgggat cgctggccca cagtcaactg
    13441 gatatcccgg aactccagcg ccgggggctg ctcattctgg cttcggagag taaggatttt
    13501 cgtctgctgg cgcatctgct aagaaccctg caacacgccg gtgatcctca tctcgcattg
    13561 cgcctgctgg cgctgtatgt ggagcattac tgggcggtgg ctgcgccgca gaatatggcg
    13621 cataaaaaac gctttgccag ccaggtcatt aaacgctttg aaacaggtat tgaagttttt
    13681 tcacagaacg ccgccacagc gcagcgcgat gcgctgtcag gggaactggc aaaactggcg
    13741 caatgctggc agtcacataa cgccccggaa ctggcacagg ctactgatga tctttttgcc
    13801 ctgtaccagc gtgcgtttaa tcgtgcggca ccggctcctg tccccactcc ggcggcctcc
    13861 ggtagttcac cacagaccac cgccacgtct gaaagcggcg tgacgcaacc cagtgctccg
    13921 gctccccaaa tcgtcatcga cagtcacgac gacaaagcct ggcgcgacac gctgttaaaa
    13981 gtggcggcca ttttatgtga acgccagccg gattcaccgc agggctaccg tctgcgccgc
    14041 catgcgcttt ggcagaacat caccagtacg ccccaggcgg aaagcgatag ccgtactcca
    14101 ctggctgcgg tctccgccga tatggtggca gattaccatg ctcagctcgg tagtgccgat
    14161 atggcgctgt ggcagcaggt ggagaaaagc gtactgctgg ctccttactg gctggatggt
    14221 cactgccttt ctgcacagac ggcgctgcgt ctgggttaca aacaggtggc agacgccatc
    14281 cacgatgagg tcatccgctt ccttgagcgt ctgccccagc ttaccgggct gctgtttaat
    14341 gaccacacac cgtttatcag tgagcagacg aaacaatggc tggcagcttc gcccgacgcc
    14401 aaagttgcac cggttgcgca aatcggtgag gaatcgaagg cagccagagc ctgttttgct
    14461 gagcagggtc tggaggcggc gctgcgatat ctggacatgc tacccgaagg cgatccccgc
    14521 gatcagtttc accgccagta ccttgccgca cagttgacgg aagaggcggg gctggtacag
    14581 cttgcgcagc agcagtaccg gatgttgttc aggatgggat tgcagatgat ggtggcagac
    14641 tgggagccgt ccctgcttga gcagctagaa caaaaattca cggcagaaca ataaaagaga
    14701 acaggagttt ttgtgttcag attaccaaca ccccgactac tcagcggact caaatcagcc
    14761 ctgcgaccgg cgatgccccg gtttaaagtc tctgctttct ggctgctgat actggcgtgg
    14821 atttttctgc tggtgtggat ctggtggaaa ggcccgatgt ggacgctgta tgaagaacag
    14881 tggctcaaac cactggcgaa ccgctggctg gcaacggcgg cgtgggggat tattgccctg
    14941 gtgtggctta ccgtccgggt gatgaagcgc ctgcaacagc ttgaaaaaat gcaaaagcaa
    15001 cagcgcgagg aagccgttga tccgctcagc gtggaactga acgcccagca gcgttatctt
    15061 gaccgctggc tgctgcgcct gcaacgccat ctcgacaacc gccgtttcct gtggcagttg
    15121 ccgtggtata tggtcatcgg cccggcgggc agtggcaaaa cgacactgct gcgcgagggg
    15181 tttccgtccg acattattta tgccccggag ggcgcacgcg gcgcagaaca acgcctgtac
    15241 ctcacgcccc atgtcggtaa acaggcggtg atctttgata tcgacggcac actctgcgct
    15301 cccgctgatg cggatatcct gcatcgccgt ctgtgggaac atgccctcgg ctggctgaaa
    15361 gaaaagcgcg cgcgccagcc gctgaacggg attattctga cactcgattt acccgatctg
    15421 ctgaccgcag acaaacgccg ccgcgagcat ctgttacaga cgctgcgcag ccgcttacag
    15481 gatatacgcc agcatcttca ctgccagtta ccggtttacg tggtactgac ccggcttgat
    15541 ttattacagg gttttgccgc cctgttccag tcactcaaca gacaggatcg cgatgcgatt
    15601 ctgggcgtca cgttcacccg ccgtgcccat gaaaatgatg actggcgaac agagttgaat
    15661 gctttctggc agacatgggt ggatcgaatg aatctggcgt tgccggatct gatggtcgct
    15721 cagactcaca cccgcgcgtc tttattcagt ttttcccgcc agatgcaggg aagccgtgaa
    15781 ccgctggtgt cactgcttga gggtctgctt gatggcgaaa atatgaacgt gatgctgcgt
    15841 ggtgtctatc tcacctcttc gcttcagcgt ggacagatgg atgatatatt cacccagtct
    15901 gccgcccgcc agtaccggct gggcaataac ccactggcgt cctggcccct ggtggacacc
    15961 gcgccttatt tcacccgcag cctgttcccg caggcattac tcgcagagcc taatctggca
    16021 acagagagcc gcgcctggct gatacgttcc cgtcgccgcc tgacggtttt ctctgccaca
    16081 ggcggcgtgg cagcactgct gctcatcacc ggctggcatc actattacaa cggtaactat
    16141 cagtccggca tcaccgtgct taagcaggcc aaagccttta tggacgtgcc gcctccgcag
    16201 ggggaagatg actttggcaa cctgcaactg ccgctcctga acccggtacg cgatgccaca
    16261 ctggcctatg gcgactgggg cgaccgcagc cgtctggccg atatgggact gtaccaggga
    16321 cgacgtatcg ggccttatg
//
LOCUS       DAZOYL010000068        16296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZOYL010000068 DAZOYL010000000
VERSION     DAZOYL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.068"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..578
                     /locus_tag="ACHUOY_001599"
     CDS             <1..578
                     /locus_tag="ACHUOY_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR0682405.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="ACHUOY_001600"
     CDS             581..1744
                     /locus_tag="ACHUOY_001600"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0682406.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="ACHUOY_001601"
     CDS             1828..3453
                     /locus_tag="ACHUOY_001601"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0682407.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHUOY_001602"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHUOY_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR0682408.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHUOY_001603"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHUOY_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR0682409.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHUOY_001604"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHUOY_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0682410.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHUOY_001605"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHUOY_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR0682411.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHUOY_001606"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHUOY_001606"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR0682412.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHUOY_001607"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHUOY_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHUOY_001608"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHUOY_001608"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR0682413.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9726
                     /gene="ulaC"
                     /locus_tag="ACHUOY_001609"
     CDS             9262..9726
                     /gene="ulaC"
                     /locus_tag="ACHUOY_001609"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR0682414.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9740..10390
                     /gene="ulaD"
                     /locus_tag="ACHUOY_001610"
     CDS             9740..10390
                     /gene="ulaD"
                     /locus_tag="ACHUOY_001610"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR0682415.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10400..11254
                     /gene="ulaE"
                     /locus_tag="ACHUOY_001611"
     CDS             10400..11254
                     /gene="ulaE"
                     /locus_tag="ACHUOY_001611"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR0682416.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11254..11940
                     /gene="ulaF"
                     /locus_tag="ACHUOY_001612"
     CDS             11254..11940
                     /gene="ulaF"
                     /locus_tag="ACHUOY_001612"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR0682417.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12037..12588
                     /locus_tag="ACHUOY_001613"
     CDS             12037..12588
                     /locus_tag="ACHUOY_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR0682418.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12663..12978)
                     /locus_tag="ACHUOY_001614"
                     /pseudo
     CDS             complement(12663..12978)
                     /locus_tag="ACHUOY_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13305..13700
                     /gene="rpsF"
                     /locus_tag="ACHUOY_001615"
     CDS             13305..13700
                     /gene="rpsF"
                     /locus_tag="ACHUOY_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR0682419.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13707..14021
                     /gene="priB"
                     /locus_tag="ACHUOY_001616"
     CDS             13707..14021
                     /gene="priB"
                     /locus_tag="ACHUOY_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR0682420.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14026..14253
                     /gene="rpsR"
                     /locus_tag="ACHUOY_001617"
     CDS             14026..14253
                     /gene="rpsR"
                     /locus_tag="ACHUOY_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR0682421.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14295..14744
                     /gene="rplI"
                     /locus_tag="ACHUOY_001618"
     CDS             14295..14744
                     /gene="rplI"
                     /locus_tag="ACHUOY_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR0682422.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14856..15827)
                     /locus_tag="ACHUOY_001619"
     CDS             complement(14856..15827)
                     /locus_tag="ACHUOY_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0682423.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15880..>16296)
                     /locus_tag="ACHUOY_001620"
     CDS             complement(15880..>16296)
                     /locus_tag="ACHUOY_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR0682424.1"
                     /translation="SWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDM
                     TPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPIN
                     KIIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3826 a         3981 c         4334 g         4155 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tcctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgagcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9421 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9481 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9541 gaagataacg acccggtgga tatcctcatc accatggcgg cggtcgatgc caacacgcac
     9601 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
     9661 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
     9721 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
     9781 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
     9841 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
     9901 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
     9961 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10021 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10081 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10141 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10201 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10261 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10321 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10381 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10441 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10501 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10561 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10621 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    10681 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    10741 ccagctggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc gtcgtcgttt
    10801 ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtga cgctggcgat
    10861 ggagatcatg gattatccgt tgatgaactc catcagcaag gcgctgggtt acgcgcacta
    10921 tctcaacaac ccgtggttcc agctctaccc ggatatcggc aacctgtcgg cgtgggacaa
    10981 cgatgtgcag atggagttgc aggccggaat cgggcatatc gtcgcggtgc atgtgaaaga
    11041 caccaaaccg ggcgtcttca aaaacgtgcc atttggcgaa ggtgtggtgg atttcgaacg
    11101 ttgcttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg agatgtggag
    11161 cgaaacggcg gaagacccgg cggcagaagt ggtgaaagcg cgtgattggg tgaaagcgcg
    11221 catggccaaa gcaggcacgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt
    11281 gaagccaaca tggatctgcc gcgctatggg ctggtgacct ttacctgggg caacgtcagc
    11341 gctatcgacc gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc
    11401 atgaaagcag acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat
    11461 cgcccgtctt ccgacaccgc gacgtatctc gaactctacc gtcgttaccc gtcgcttggc
    11521 ggcattgtcc atacccactc cactcatgct actgcatggg cgcaggcggg gctggcgatc
    11581 ccggcgttag gcactacgca cgccgactac ttctttggcg acatcccgtg tacgcgcgga
    11641 ttaagcgaag aagaggtgca gggcgagtat gaactgaaca ccggcaaagt gattatcgaa
    11701 acgctgggtg acgccgagcc gctgcatacg ccggggattg tggtgtatca gcacggtccg
    11761 ttcgcctggg ggaaagatgc ccacgacgcg gtgcataacg cggtggtgat ggaagaagtg
    11821 gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac tgaatcacat cgacagcttc
    11881 ctcatgaata aacacttcat gcgtaagcac ggtcctaacg cctattacgg gcagaagtaa
    11941 gcagtgcgcc ccggaattat tacctcctgc gggatgtaat tggtctgatt ccggggccgc
    12001 ttcagttaaa aaatcgtata aacaacaggt gtttttatgg ctatcaatgg attgtttgtc
    12061 agaacggatc cgcaacgtcg ccgctatggc gttgcgttac ttgtgggtgt actttcagga
    12121 attatttcag catttgttaa atggggagcg gaagtcccct tgccaccgcg cacattttcc
    12181 ggtggtcgcg atgaatttaa cccgccgtat atctttctac gggattattt aggtatcgac
    12241 ccaacccaaa ctgtgtatac attctcggaa catattatca acccggtgat ggtgacgcat
    12301 atcatcttct cactggtttt tgcgataggg tattgtattg tcgcggaaat tttcccgaaa
    12361 gtgaaattat ggcaggggat attagcaggg cttattgtga cagtggcagt ccacggtata
    12421 ttttgcccgg cgctgaattt aacgccgccg ctcactcaat tgccttttga cgaatatgcc
    12481 tctgaaatcc tggggcatat tttctggttc tggataatag aaattatgcg ccgcgactta
    12541 cgtaatcgca ttactcatga gccagatccg gaagtcgcaa tccggtgaat aaccgcataa
    12601 aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg
    12661 tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg ttcccgcatc
    12721 agaattacgt ggtagtacgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt
    12781 tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa
    12841 attatgattg atataaatca cgcctaagct ttgcacatca tccatgtttc tggcctggtg
    12901 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
    12961 taaaactcga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg
    13021 atgatcttct gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg
    13081 cagttgttca aaaaaacacc atgatgaagt gtgatgaact ccaaatcagc ggtttagagg
    13141 ttaattgcga aaggggagat ttatttcggc tctgcccttg agtttagcgg ggcatacaag
    13201 tactataacg cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg
    13261 gctgacccag acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa
    13321 tcgtttttat ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca
    13381 ctgctgccat cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc
    13441 agctggctta cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag
    13501 ctccgcagga agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc
    13561 gcagcatggt tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga
    13621 aagacgagcg ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag
    13681 ctggggattc tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt
    13741 gcaggactcc ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg
    13801 agcatcgttc tgtgcaggag gaagccggtt ttcaccggca ggcgtggtgc caaatgcccg
    13861 ttattgttag cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca
    13921 taaccgttca ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt
    13981 tgcatgccga gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc
    14041 cgtcgtcgca agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat
    14101 atcgctacgc tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc
    14161 ggtacccgtg caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg
    14221 tccctgctgc cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt
    14281 gagaggataa ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg
    14341 gtgatcaggt aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag
    14401 ctgttccagc taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag
    14461 ctaaactggc tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg
    14521 aaactgttac catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta
    14581 ctcgcgacat cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc
    14641 gtctgccgaa cggcgttctg cgtaccactg gcgagcacga agtgagcttc caggttcaca
    14701 gcgaagtatt cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag
    14761 acgtaaaaac gccgaccagt ggtcggcgtt ttgcttttgt agcgagcact tgcagatatc
    14821 gataagggcc acaataagca gccctacgaa gataattact tagctgcctg aatatcagcc
    14881 agcattgggg caagggctgg gttgtcctta gcgaagtttg cgtacattgg ctgtactttc
    14941 tcgataaacg gagctttatc tacctggtag actttcacgc ccattttatc cagttttgca
    15001 aattcttgtt tctcttcctc gctccacaag tctttcatgt agctcattga ttctttagct
    15061 gcttttaata taactgcctg attttccggg ctaagtttat caaacgcagc attgctcatc
    15121 agaaccacat cgggaaccat agtgtgttcg tcaaggctga aggattttgc cacttcaccg
    15181 tgacgcatgg tggtcagcgc catcacgctg ttttctgcca tatcgaccac accctgctgt
    15241 aaggctgtat acaactcgcc gtaatccagt ggcgtcgggt taccgcccat gactttgatc
    15301 atttcaactg cgccagggct tggctggaca cggactttca tgcctttcag atcgtctggt
    15361 ttcagtactg gtttattgcc atagaaactg cgggcgcctc catcataaaa agtcagcccg
    15421 aaataacctt tgcttttcgt tgagtcgagg atttttctcc cgatatcgcc ctgcatgacc
    15481 tgataataat gatcacgatc gcggaacaaa taaggcaggc taaataattt ataggaattt
    15541 tcaaatgctt ccatttctgc cgcattggtt tttaccagtg gaatagcgcc agaacgaatc
    15601 agctccattg tttctcgttg agtacccaat gttccatttg gataaatacg aatagtaata
    15661 tcaccgttag agtactcttt gttctttttc gcaaagaact ccatcgcttt atgaacagga
    15721 tgagacttat cctgattatg actcagtttt aatgtcactg cccaggactg gcttgcggat
    15781 aacataagaa cagcaccgat aatgccagcg gatactttta ttttcataga tgttcctttt
    15841 ctaataaata tggcggcagg tgccgccagt gaatgcgcat cagtagccca gtgcttttgg
    15901 caatgccata ctgatttgcg gaatataggt caccattgcc agcaccaata ccatcacggc
    15961 atagaatggc agcataggtt tgataatttt attaatcggt aatttagata cactacaacc
    16021 gacaaataac acactgccta caggcggagt acaaataccg atacacatgt tcagtaccat
    16081 gacaatacca aagtgaatcg ggtccatgcc cagttcggtg acaacaggta aaaatatcgg
    16141 cgtaaaaatc agtaccgccg gtgtcatatc cataaaagtg ccgataatca acaggataat
    16201 attgataata agcaggatta ttattgggtt gtcagaaata gtgttaagca aatcgagaat
    16261 caggaaggga acgtcagcgt ttgacattgc ccatga
//
LOCUS       DAZOYL010000069        15849 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZOYL010000069 DAZOYL010000000
VERSION     DAZOYL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15849
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.069"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1861)
                     /gene="htpG"
                     /locus_tag="ACHUOY_001621"
     CDS             complement(<1..1861)
                     /gene="htpG"
                     /locus_tag="ACHUOY_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR0682425.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQ"
     gene            complement(1971..2576)
                     /gene="recR"
                     /locus_tag="ACHUOY_001622"
     CDS             complement(1971..2576)
                     /gene="recR"
                     /locus_tag="ACHUOY_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR0682426.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(2576..2905)
                     /locus_tag="ACHUOY_001623"
     CDS             complement(2576..2905)
                     /locus_tag="ACHUOY_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR0682427.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(2958..4883)
                     /gene="dnaX"
                     /locus_tag="ACHUOY_001624"
     CDS             complement(2958..4883)
                     /gene="dnaX"
                     /locus_tag="ACHUOY_001624"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR0682428.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    complement(3561..3625)
                     /gene="dnaX"
                     /locus_tag="ACHUOY_001624"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(5012..5563)
                     /gene="apt"
                     /locus_tag="ACHUOY_001625"
     CDS             complement(5012..5563)
                     /gene="apt"
                     /locus_tag="ACHUOY_001625"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR0682429.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(5716..6093)
                     /locus_tag="ACHUOY_001626"
     CDS             complement(5716..6093)
                     /locus_tag="ACHUOY_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR0682430.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            6163..6690
                     /gene="priC"
                     /locus_tag="ACHUOY_001627"
     CDS             6163..6690
                     /gene="priC"
                     /locus_tag="ACHUOY_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR0682431.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            6704..6865
                     /gene="rsmS"
                     /locus_tag="ACHUOY_001628"
     CDS             6704..6865
                     /gene="rsmS"
                     /locus_tag="ACHUOY_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR0682432.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(6918..7016)
                     /locus_tag="ACHUOY_001629"
                     /pseudo
     CDS             complement(6918..7016)
                     /locus_tag="ACHUOY_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7077..10439)
                     /gene="mscK"
                     /locus_tag="ACHUOY_001630"
     CDS             complement(7077..10439)
                     /gene="mscK"
                     /locus_tag="ACHUOY_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR0682433.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(10567..11214)
                     /gene="acrR"
                     /locus_tag="ACHUOY_001631"
     CDS             complement(10567..11214)
                     /gene="acrR"
                     /locus_tag="ACHUOY_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR0682434.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            11356..12549
                     /gene="acrA"
                     /locus_tag="ACHUOY_001632"
     CDS             11356..12549
                     /gene="acrA"
                     /locus_tag="ACHUOY_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR0682435.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            12572..15721
                     /gene="acrB"
                     /locus_tag="ACHUOY_001633"
     CDS             12572..15721
                     /gene="acrB"
                     /locus_tag="ACHUOY_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR0682436.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         3655 a         4212 c         4188 g         3794 t
ORIGIN      
        1 gctggttcat acgacgaata aacaggttcg gatcttccag cgtgccgcgt tctgccagca
       61 gcgcctgatc cagcagcagt tctacccact cgctgaactt cgcttcatct tcagtatctg
      121 ccgcacgttt caccagtacg tgatccgggt tcagttcgaa gatgtatttc acttctggca
      181 ctttctggcc cgccgcagcg aacagtttcg ccatctgagt gctcatttcg tccgcgtcgg
      241 tcgaaacgat cgctggcgta tcggtcagac ggtgagtcag acggacatct ttcacgcgct
      301 cgccgagcag ggctttcaca cggtcgatga acggagtcag tgctttctcc gcttctttcg
      361 cgctctcatc aacttcgtca gccagttttt ccagcgactc gtcaacttta gacaccgact
      421 ggaacggttt accgtcgaac tcagtcagat agttcatcat ccactcatcg atgcggtcgg
      481 aaagcagcag aacttcgatg cctttcttac gcagcagttc caggtgcggg ctgctcttcg
      541 ctgccgcata gctgtctgcg gtgatgtagt agattttctc ctgcccttct ttcatgcggg
      601 aaacgtagtc ttccagagat acggtctgcg cagaagaatc ggtatgggta gaagcaaaac
      661 gcagcagttt ggcgatcgct tcctggttag cgaaatcttc cgccggacct tctttcagta
      721 ccaggccaaa ctgttgccag aaggtctggt atttttccgc gtcgtctttc gccagttttt
      781 ccagcatttg cagcacacgc ttggtcagcg cattgcgcag gttacgcgtt accgtgctgt
      841 cctggaggat ttcacgggaa acgttcagcg gcagatcgct ggagtcaatc agaccacgca
      901 cgaagcgcag atagttcggc atgaactgtt ctgcgtcgtc catgatgaac acacgctgga
      961 cgtacagttt caggccgtgt ttatgatcac ggttccacat atcccacgga gcctgggacg
     1021 gaatgtacag caggctggtg tactcctgct taccttcaac gcggttgtgg ctccaggtca
     1081 gcggatcatt aaagtcgtgg gcgatgtgtt tgtagaactc tttgtactct tcatcggtga
     1141 tttccgactt gttacgagtc cacagcgcct gcgctttgtt gattttctcc caggagataa
     1201 cggtttcgcc gtctttctct tcgcgttttt cgatctctac cggcagcgcg atatggtcgg
     1261 agtatttgct gatgatggaa cgcacgcgcc agtcatcgag gaactcgtct tcgccttcac
     1321 gcagatgcag ggtgatttca gtaccacgat cttctttggt gatgtcggca acggtatatt
     1381 caccttcgcc agccgattcc cagaagacgc cgttttctgg tttttcgcct gccgcgcgag
     1441 tacgcacggt cactttgtcg gccacgataa acgcagagta gaaaccaaca ccaaactgac
     1501 cgatcagctg gctgtctttc gcctggtcag aacccaggga ttcgaggaat gatttggtac
     1561 cggatttagc gatagtcccc agatggtcaa tcacttcgtc gcgggtcatc cccacgccgt
     1621 tatcagagat ggtcagcgtg cgcttgtctt tatcgaaaga gacacgaacg cgcagttcgc
     1681 catcaccttc gtacaggtcc gggttagata gcgcacggaa acgcagcttg tccgccgcat
     1741 cggaggcgtt agagataagc tcacgcagga agatttcttt attggaatag agagaatgga
     1801 tcatcaggtg cagaagctgt ttcacttctg actgaaaacc acgagtttct tgtcctttca
     1861 tgtaggtcta cctcaataat gccattttaa ggttaaaaac aggatgaggg agagatgggg
     1921 acaagggaga ataatttcaa gcgagagcag gtgatcctgc tctcgtttgc ttaaaaacga
     1981 atcttatgac gcccggcaag ggagtgtgac agcgtggtgc catcgaccat ttccagttcg
     2041 ccgccaaccg gtacgccatg agcgattcgg ctggcttcca cgtcatattg cgcgcaaagc
     2101 tcggcaatgt agttagcggt agcttcacct tcaaccgtgg gattggtggc gaggatcact
     2161 tcagtgattt tttcctctgc cagacgctgt tccagacgat caagcccgat atcatccgga
     2221 ccgatgccgt ccagcggtga cagatgcccc atcaacacaa aataacgacc agaaaactgc
     2281 cccgtctgct caatggcgta gatgtccgcc ggactctcca ccacacagat ttgaccgttt
     2341 tcctgacgac gcggattcga acagatgtta cagacttcct gttcggtgaa agtgcggcaa
     2401 tcggcgcagt ggccgatttc cgacatcgcc cgggtgagcg cctgcgccaa acgcatcccg
     2461 ccgctacgat cgcgctgaag cagcgtgaac gccatacgct gcgccgactt cgggccaacg
     2521 cccggcagac agcgcagtgc ttccataagc tgtgttaaca gcgggctggt ttgcatcaga
     2581 acggcatctt aaagccaggc ggcagctgca ttccggagga tacagaggcc attttttctt
     2641 tctgcgtttc ttcaatacga cgtgctgcgt cgttgaatgc tgcagccacc aggtcttcca
     2701 gcatctcttt gtcgtcttcc agcaggctcg ggtcgatctc tacgcgacgg cagttgtgtg
     2761 caccgttgat ggtcactttt accagacctg cgccagattc gccggtgact tccagctgcg
     2821 cgatctcttc ctgcattttc tgcatttttt cttgcatctg ctgggcttgc ttcatcaggt
     2881 tacccagacc gcctttacca aacataggtt tctctctcaa tcacgttaag gatgacgaac
     2941 gtaagctgtg cttacgatca aatggggcgg atactttctt catccagctc cgcatcgaaa
     3001 aaccgacgca gggtctgaat attattatcc gcaataatgg actcgcgcgc ctgcgcaagt
     3061 ttttcttcgt atatcgcctg acgccactcc agcggcgtac gcaccgcggg attatcatct
     3121 tcaacgatag tcagttcaac cgttgaacct tttaacatgc tcaacgcttc agccagtttt
     3181 tgctgtgcac cgcggttgtt caaatgccgc tgagaggagc gcaaatgcag acatactgcg
     3241 ttgtcgctct cctctttcca ggcatttaac gccacctgtt cgaccagttt tggtagcgaa
     3301 agttggctca cctgtgccgc ccacgggtcg cgctcaatgg cttctgccgc tagcttcgcc
     3361 gccagttccg gcgttttttc atgttccagc gcttttttca gcgccttcgg cgtggcgacc
     3421 acttcttttt gctgcatcac cggagtggtc gccttccagc gatacgcttc ttttttggct
     3481 gacgcttttt ccagcgccga tggcaccgga cgcgcctgaa cgcgatcggt gaccgaagcc
     3541 agtctttcca gcgcagcgtt attcaccggc cgcgcgcggg tagcggctgc cgattcactc
     3601 ttttttgctt tggttgctcc ctgcacgcac tgcaactgct ggcgcgccgc cagcacctgg
     3661 ctggtggttt ccgggagcgg tacagtcggt gcctgctgcg gcgctgattg cggcggcacc
     3721 tgggttggcg tcattactgc cgttggcacg acgggtgcaa aggactggcg tggcacttct
     3781 ggctcaggca gcggcatacg cggatggaac gccagcgcgc gcagcagcgt catctcaacg
     3841 cccatgcggc ggtccggcgc atacggtaat tctttgcgac caatcaacag cgtctgatag
     3901 taaagctgaa tatccgtcgg cggtatggtg cgcgccagtt cacgcatccg cagctcaatg
     3961 gcggccatgt cgttgccaag tgcagcaggc gaaagttgta ccatcgcaat acgatgcaac
     4021 aggccgagca tttccaccag caacgcttcc cactcgatac cacgggcagc ggcttcatta
     4081 atcagcgcca ttacgcgctc gccgttggcc tcgaccatcg cttcaaccag cgacagcgcc
     4141 tgatcgtcat caagcgtacc cagcatcgca ctgactgcct gggttgaaac ctggccgtca
     4201 ccgctggcaa tcgcctggtc ggtcagactt aaggcatctc gcaggctgcc ttcagcggcg
     4261 cgcgccagca attgcaacgc ccgcggctcg tgagcgatat gttcttcgtt gaggatgtgc
     4321 tcaagttgat ggcgaatttg ctcgacatcc agcgccttga gatgaaattg caaacagcgc
     4381 gacaaaatcg tcaccggcaa tttctgtgga tcggtcgtcg ccagcagaaa cttaacgtgc
     4441 tccggcggct cttcaagggt ttttaacagt gcgttaaagc tgtggcgcga cagcatatgc
     4501 acttcgtcga tcagataaac tttgaaacga ccacgcgccg gagcgtactg gacgttatcc
     4561 agcaggtcgc gggtatcttc aactttggtg cgcgaggcgg cgtcgatttc aatcagatcg
     4621 acaaagcgcc cctgctcgat ttcacgacag ttatcgcaca cgccgcacgg cgtcgcggta
     4681 atgccggttt cgcagtttag ccccttcgcc agcagtcggg cgatagaggt ttttccgacg
     4741 ccacgggtgc cggaaaaaag ataagcatga tgaatacgcc ctaacgacaa gccgttcgcc
     4801 agtgcggtca gcacatgttc ctggccgacg acgtcagcaa aggtttgtgg gcgccatttt
     4861 cgggctaaga cctgataact cattggcagg ctctgaaacg ctggaaggtg gattcacgaa
     4921 ggggtaatgc taacacagcc ccgtcagaac ggcgaggctg aatgcacatc ttgtgctgtc
     4981 cgtagcgtgg gcagcacaag actggcgata attaatggcc cgggaacggg acaaggctgt
     5041 agctggtaat gccctgtttt tcgagacgct gttcgccgcc gagatcgaac aggttgataa
     5101 tgaacgcagc gtcagccact tcaccaccca gacgacggat cagtttaacg gtcgcttcga
     5161 tagtgccgcc ggttgccagc agatcgtcca ccaccagaac tttgtcgccc ggtttgatgg
     5221 catcaacgtg gatctccagc tgatcggtgc cgtattccag gtcgtaagtt tcactgatgg
     5281 tttcacgcgg cagtttgccc ggtttacgga ccggtacaaa gccaacgccc agacccagag
     5341 ctaccggagc gccaaacaag aagccacgcg cttcggtgcc gacaactttg gtaatgcccg
     5401 catttttgta acgctcaacc agcaagtcga tgctgagagc gtaagctttc gggtcttcca
     5461 gtaagctggt gacatcgcgg aaaagaatgc cgggttttgg gtagtcctga atgcttttga
     5521 tgctattttt gagatactca agctgctgtg cagtcgcggt cataagtgta tgcctgcttg
     5581 ttacggtggt actcacggcg cgttttaaac gtatcaaaag taacagttgt ttaacctttg
     5641 accgcctgcg cctgtgctcg aaaacgccag aatgtactgg ctgtacgcaa taattgcaac
     5701 tgcgattgtt gtgcttcagt gcttttcttg cttttcatca atcaccggaa ttcgccacat
     5761 atagaaaagc aaacaggcga gaattaccag caacatgatg cgcacccatg gcatctggac
     5821 gaaccacaga gaaatggcaa acgtgagcaa aataagcaaa atcgcccgcg gtttgacgcc
     5881 gcgcggcatc gcatgatgtt tctgccagaa acgtagatag ctgccaaacc atgagcggta
     5941 cagcaaccag gcgtgaaagc gcggggaaga acgggcaaag caccaggccg ccagcaggat
     6001 aaacggcgtc gtcggtaata ccggtaatac cacgcccagc gtacccagca ctaccgccag
     6061 ccagccaatg atgattaaaa tgattcgttg catattgagt gttgtcagct tacagagtgg
     6121 ctactttagc ataacaatta tcattttcat tgaggtctta tcgtgaaaac cgccctgctg
     6181 ctggaaaaac tggaaggaca gctcgctacg ctgcgtcagc gttgtgcccc ggtgtcacag
     6241 ttcgccacgc taagtgctcg tttcgacagg catctttttc agactcgtgc gacaacacta
     6301 caggcttgtc tcgacgaggc gggcgataat ctggctgcgc tttgtcatgc ggttgagcag
     6361 caacagctgc cgcaagtggc ctggctggcg gaacatctgg cggcacaact ggaagccatc
     6421 gcgcgtgaag cctccgcctg gtcattgcgc gagtgggaca gtgcgccacc gaaaattgcc
     6481 cgctggcagc gtaaacgtat tcagcatcag gattttgagc ggcggctacg tgagatggtt
     6541 gccgaacgca gagcccgcct ggcgcgggtg accgatctcg tggaacagca aacgctgcat
     6601 cgtgaagtgg aagcctatga agcgcgcctg gcacgctgcc gccatgcgct ggaaaaaatc
     6661 gaaaacaggc tagcgcgctt aacccgctag caatggagag agtatgtcac ttgaaaatgc
     6721 ccctgacgat gtcaaactgg ctgtcgattt gattgtgctg ctggaagaaa atcagatccc
     6781 agcccgcacc gtgctgcgcg cgctggatat tgtaaaacgc gattatgaaa agaaattaac
     6841 gcgcgatgat aaggcggaaa agtgagaaat aaatggggcc gctaaatgtc ccctaagatt
     6901 gtgggcaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacacc gctcctgcaa
     6961 tacattgaat tcttgcggtt tcgtaggccg gataaggcgg tcacgccgca tccggcatga
     7021 acaacgcgca ctttgtcagt aatctcgggg caactaaatg tcgccccctt tggttattac
     7081 cctaccgctg gcgtcgggtc atcgcctttg tagtcgcgtt tgacttccgt cacctcatcg
     7141 cccttctcgt tgtgcagatg cacttcaagc tggttaaagg caatgttgat gtcgttttca
     7201 cggcacagct gatcgatagt acggttcagc tcatcgacag tacgactacg gtcacgcagt
     7261 tcacgcacat acagacgcag ctcatgatcc aacgtgctgg caccaaacgc cgtaaagaag
     7321 acttccggca ttggttcgtg catcaccctt gggtgctcag tcgccgcctt cagtaacact
     7381 ttacgcactt tttccagatc ggagccatag gccacgccga gacggatcac cagacgcgta
     7441 gtagtgtcag tcaacgacca gttgatcaga cgctcggtaa caaacgcttt gttcgggatg
     7501 atcacttctt tgcgatcgaa atcggtaatc gttgtcgcac gaatacggat cttacttacc
     7561 gtccccgaga agctaccaat ggttaccgta tcgccaatac gcaccggacg ttcgaacaga
     7621 atgatcaaac cggagacgaa gttaccgaaa atttcttgta aaccaaaacc aagacctacg
     7681 gataatgctg cggccagcca ctggagttta tcccaagaga cgcccagcga tccgaacacc
     7741 gtcatcgcac caacggcaat aatgatgtag ttaaggatgg tagtaatggc atacgacgcg
     7801 ccctgacgca tattcagtcg cgagagcacc agcacttcca gtaaaccagg caggttgcga
     7861 atcaacgccc aggccaccat tgaggcgata atcgcaaaca acagactgcc catggtgacg
     7921 tttttcacca ccgcagcgcc agcttcagtg ccgttgtaat gccagagcgt gatgctgtcg
     7981 agatagctga acacggtgat caaatcggac caaattgccc agaacatgac accgaacagc
     8041 gcaaacatca gcaacatggt aatacgcagc gtctgctggt taacctgttc cagtgcaatg
     8101 gtgggttctt ccggcggttc ggcaccttct gcgccctctt tcaccagatt ctgccgacgc
     8161 gccagcgcac gacgccaggc gatacgccgc gccgctacgc ttaagccgcg caataccgtc
     8221 tggtagagca agttccagat gatcaccaga taaacggttt caatccaacg cccggagagg
     8281 cgcagcgtgg tgtagaaata acccgtggca gtcagcacca tcaatgcaat cgggataatc
     8341 gacagcacgg taatggtgac cagtcgcatg gtgtgcgact ctttatcacg ccagctttcg
     8401 cggcacattg gccataccag gaaggcaatc agcagcaggt tgaagaaaat catcgcctgc
     8461 cccagcacat catccatcag atgcagcggg gaaagttctg ccaccacaga ccagaaatgg
     8521 ataggcagca acgcgagact gatgcggaca atttgccgac gccagtggct ggtctgctgc
     8581 tccggcatgc cgaagtgacg cacggcaacg ccatttttct ccagtacctt ccagcacagg
     8641 ccaaacacca gccagaaaat cgccagtttt ttactgaacg accatagcag ttcgctgatg
     8701 ttgagctgca tggtcaacag aatcaggcca accgcgagaa taatcaggca caccggcagc
     8761 gcgcggatca ggtcgataag gatcgctttt ggtgtgttga gctggctatc gttacgcagg
     8821 gaacccacag cggaagccag tttctgttga tacgctttca gccagcccag acgccagtgg
     8881 atcagcccgg caatcaacag cagcggcaaa ccggcgagga aagcgataaa aacggcaggc
     8941 caggcttttt cccagttcac cgtgattttc atcgacttaa attcatcttt caggctttgc
     9001 gggaacgctt tgatccagtc ccagtccatt ggacggttac tgttcaccca aaagatttgc
     9061 tgagtcagga tggatttcag gtttttcgat acgctcatta actgctgctg gttgatttgc
     9121 aggttaatgg ccatcatcag ctggttaccc aactgtttgt tgagttgatc cagcaattcg
     9181 cgacgcatat caaccacttg caataacgca tcgtgaactt cgctgttgac ttcgttggtg
     9241 tgaccttctt ccagtttgtt gacgaacgca tcgctctgga agagtgcatc acgctgctgg
     9301 ttaacttcaa actgttcgag acgcaaatcc gcgatgcggt tggtcatgtt ttccagttca
     9361 tccgccgagg gcagcgtttg ttgttgctgg taaaggatac gagacaacag caggctgccc
     9421 ttcaggacgg caatctgctc tttaatattg cgttccgatt gcagcgcccg ctccagccag
     9481 tttttgactt taatgttttg ctgcatcaac tgattaccgt tttcagtcgc ggtaatcaga
     9541 cgctgactta actgctggtt aatttccagt tcctgtttca ccagcggatt agcctgaata
     9601 cgcgcggctt catccgggga gacggcttcc tgcgccgttt tttcagttaa agtcaggcgc
     9661 ttgctgttta ccgcttcttg caacagctgc aactggtgct ccagacgggc gctgttcgcc
     9721 gtcacgtaat cacgttgctt ttgcaaggta tcctgcaaga cggtgttccc ttccaggctt
     9781 ttacgctgct ggtcaatctc cgcattcagc aacgcctgct gagcctgcat taacactttc
     9841 tggctgggac gtaaggcagt ctcgccgaca tcagtcccat ccagacgact gcgaatttgt
     9901 tgcagctgct gcgaagcgtt atacatcgca ttttgcacgc gttcgggctg cgtctgtaac
     9961 gaaaccagct ggctgttata agacgccaga tcgttttgtg cgttttgcaa atcgtccagc
    10021 gcctgggcaa cgcgagtttc cagctggcgc aacgaaagcg tactcagaat tttgcgcgtt
    10081 tcttcgtcgt tatcgacatc gctaagtgct gttaacgccg cggtcgcctg gcgcattttt
    10141 tccggcgctt cagcgacttt ttgccgtagc tgaactgtct cttcttttat gcgatcgatt
    10201 ttatcgaggg tggctaatgt atctgtcaga tcctgctgca ccagtttgtc ctgagcagaa
    10261 agatcttttt gtttatttag tgagtcaagt tgcgcctgca ggtccgcttt tgtcggcaga
    10321 tcaccattcg atgacgcccg cgcaaacgcc gtgttctggc ataacaagac aaaaacaaaa
    10381 gcaataaatg ctgaaaaaac aaaatgccgt gatcgtttgt aatactggaa catagtcatg
    10441 atgaatgaag gtttctgaac ctgaagaacg acctgaaaaa gtcaaaccgc aagaatatca
    10501 cgacgcagtg aaccagaata gcaacgacga aaatgtccag gaaaaatcct ggagtcagat
    10561 tcagggttat tcgttagtgg caggattacg aagcgtgggg cacaggagat acatctccag
    10621 taagatggcg acgtaatcgc gggcttcttt tttaagatca aaagattgcg gggcaaagag
    10681 ccagttttcc atcaggccgg aaatatagcc gcgcataata attgctgccc gacgcgtcat
    10741 taaatccgca ggcaacattt tcgcttcaat acaatgtttt aacgtttgtt ctatacggtc
    10801 ataactttcc agacagagat tacgttgtgc ctgttgcaca acagccattt ctccgacaaa
    10861 ttcgcatttg tggaatataa tctccatcaa taatcgacgt cgttcttctg tcaccgtgga
    10921 ttcaagaaca tgaattaata tctctcttaa tactgagagt ggatcgccag ggaattttgc
    10981 ctgatactca agctctagtt caccaatatt ggattctgac agttcccaga tctcactgaa
    11041 caaatccgac ttgtctttaa aatgccagta gattgcaccg cgcgtaacgc cagctgcttt
    11101 tgcaatctcg cccagcgagg tggatgatac cccctgctgt gagaaaagac gtagagccac
    11161 atcgaggatg tgttggcgcg tttcttgcgc ttcttgtttg gtttttcgtg ccatatgttc
    11221 gtgaatttac aggcgttaga tttacataca tttgtgaatg tatgtaccat agcacgacga
    11281 taatataaac gcagcaatgg gtttattaac ttttgaccat tgaccaattt gaaatcggac
    11341 actcgaggtt tacatatgaa caaaaacaga gggtttacgc ctctggcggt cgttctgatg
    11401 ctctcaggca gcttagccct aacaggatgt gacgacaaac aggcccaaca aggtggccag
    11461 cagatgcccg ccgttggcgt agtaacagtc aaaactgaac ctctgcagat cacaaccgag
    11521 cttccgggtc gcaccagtgc ctaccggatc gcagaagttc gtcctcaagt tagcgggatt
    11581 atcctgaagc gtaatttcaa agaaggtagc gacatcgaag ctggtgtctc tctctatcag
    11641 attgatcctg cgacctatca ggcgacatac gacagtgcga aaggtgatct ggcgaaagcc
    11701 caggctgcag ccaatatcgc gcaattgacg gtgaatcgtt atcagaaact gctcggtact
    11761 cagtacatca gtaagcaaga gtacgatcag gctctggctg atgcgcaaca ggcgaatgct
    11821 gcggtaactg cggcgaaagc tgccgttgaa actgcgcgga tcaatctggc ttacaccaaa
    11881 gtcacctctc cgattagcgg tcgcattggt aaatcgaacg tgacggaagg cgcattggta
    11941 cagaacggtc aggcgactgc gctggcaacc gtgcagcaac ttgatccgat ctaccttgat
    12001 gtgacccagt ccagcaacga cttcctgcgc ctgaaacagg aactggcgaa tggcacgctg
    12061 aaacaagaga acggcaaagc caaagtgtcg ctgatcacca gtgacggcat taagttcccg
    12121 caggacggta cgctggaatt ctctgacgtt accgttgatc agaccactgg gtctatcacc
    12181 ctacgcgcta tcttcccgaa cccggatcac actctgctgc cgggtatgtt cgtgcgcgca
    12241 cgtctggaag aagggcttaa tccaaacgct attttagtcc cgcaacaggg cgtaacccgt
    12301 acgccgcgtg gtgatgccac cgtactggta gttggcgcgg atgacaaagt ggaaacccgt
    12361 ccgatcgttg caagccaggc tattggcgat aagtggctgg tgacagaagg tctgaaagca
    12421 ggcgatcgcg tagtaataag tgggctgcag aaagtgcgtc ctggtgtcca ggtaaaagca
    12481 caagaagtta ccgctgataa taaccagcaa gccgcaagcg gtgctcagcc tgaacagtcc
    12541 aagtcttaac ttaaacagga gccgttaaga catgcctaat ttctttatcg atcgcccgat
    12601 ttttgcgtgg gtgatcgcca ttatcatcat gttggcaggg gggctggcga tcctcaaact
    12661 gccggtggcg caatatccta cgattgcacc gccggcagta acgatctccg cctcctaccc
    12721 cggcgctgat gcgaaaacag tgcaggacac ggtgacacag gttatcgaac agaatatgaa
    12781 cggtatcgat aacctgatgt acatgtcctc taacagtgac tccacgggta ccgtgcagat
    12841 caccctgacc tttgagtctg gtactgatgc ggatatcgcg caggttcagg tgcagaacaa
    12901 actgcagctg gcgatgccgt tgctgccgca agaagttcag cagcaagggg tgagcgttga
    12961 gaaatcatcc agcagcttcc tgatggttgt cggcgttatc aacaccgatg gcaccatgac
    13021 gcaggaggat atctctgact acgtagcggc gaatatgaaa gatgccatca gccgtacgtc
    13081 gggcgtgggt gatgttcagt tgttcggttc acagtacgcg atgcgtatct ggatgaaccc
    13141 gaatgagctg aacaaattcc agctaacgcc ggttgatgtc attaccgcca tcaaagcgca
    13201 gaacgcccag gttgcggcgg gtcagctcgg tggtacgccg ccggtgaaag gccaacagct
    13261 taacgcctct attattgctc agacgcgtct gacctctact gaagagttcg gcaaaatcct
    13321 gctgaaagtg aatcaggatg gttcccgcgt actgctgcgt gatgtggcga agattgagct
    13381 gggtggtgag aactacgaca tcatcgcaga gtttaacggc caaccggctt ccggtctggg
    13441 gatcaagctg gcgaccggtg caaacgcgct ggataccgct gcggcaatcc gtgctgaact
    13501 ggcgaagatg gaaccgttct tcccgtcggg tctgaaaatt gtttacccgt acgacaccac
    13561 gccgttcgtg aaaatctcta ttcacgaagt ggttaaaacg ctggtcgaag cgatcatcct
    13621 cgtgttcctg gttatgtatc tgttcctgca gaacttccgc gcgacgttga ttccgaccat
    13681 tgccgtaccg gtggtattgc tcgggacctt tgccgtcctt gccgcctttg gcttctcgat
    13741 aaacacgcta acaatgttcg ggatggtgct cgccatcggc ttgttggtgg atgacgctat
    13801 cgttgtggta gaaaacgtgg agcgtgttat ggcggaagaa ggtttgccgc caaaagaagc
    13861 tacccgtaag tcgatggggc agattcaggg cgctctggtc ggtatcgcga tggtactgtc
    13921 ggcggtattc gtaccgatgg ccttctttgg cggttctact ggtgctatct atcgtcagtt
    13981 ctctattacc attgtttcag caatggcgct gtcggtactg gtagcgttga tcctgactcc
    14041 ggctctttgt gccaccatgc tgaaaccgat tgccaaaggc gatcacgggg aaggtaaaaa
    14101 aggcttcttc ggctggttta accgcatgtt cgagaagagc acgcaccact acaccgacag
    14161 cgtaggcggt attctgcgca gtacggggcg ttacctggtg ctgtatctga tcatcgtggt
    14221 cggcatggcc tatctgttcg tgcgtctgcc aagctccttc ttgccagatg aggaccaggg
    14281 cgtatttatg accatggttc agctgccagc aggtgcaacg caggaacgta cgcagaaagt
    14341 gctcaatgag gtaacgcatt actatctgac caaagaaaag aacaacgttg agtcggtgtt
    14401 cgccgttaac ggcttcggct ttgcgggacg tggtcagaat accggtattg cgttcgtttc
    14461 cttgaaggac tgggccgatc gtccgggcga agaaaacaaa gttgaagcga ttaccatgcg
    14521 tgcaacacgc gctttctcgc aaatcaaaga tgcgatggtt ttcgccttta acctgcccgc
    14581 aatcgtggaa ctgggtactg caaccggctt tgactttgag ctgattgacc aggctggcct
    14641 tggtcacgaa aaactgactc aggcgcgtaa ccagctgctt gcagaagcag cgaagcaccc
    14701 tgatatgctg accagcgtac gtccaaacgg tctggaagat accccgcagt ttaagattga
    14761 tatcgaccag gaaaaagcgc aggcgctggg tgtttctatc aacgacatta acacctctct
    14821 gggcgctgca tggggcggta gctatgtgaa cgactttatc gaccgcggtc gtgtgaagaa
    14881 agtttacgtc atgtcagaag cgaaataccg tatgctgccg gatgatatcg gcgactggta
    14941 tgttcgtgct gctgatggtc agatggtgcc attctcggcg ttctcctctt ctcgttggga
    15001 gtacggttcg ccgcgtctgg aacgttacaa cggcctgcca tccatggaaa tcttaggcca
    15061 ggcggcaccg ggtaaaagta ccggtgaagc aatggagctg atggaacaac tggcgagcaa
    15121 actgcctacc ggtgttggct atgactggac ggggatgtcc tatcaggaac gtctctccgg
    15181 caaccaggca ccttcactgt acgcgatttc gttgattgtc gtattcctgt gtctggcggc
    15241 gctgtacgag agctggtcga ttccgttctc cgttatgctg gtcgttccgc tgggggttat
    15301 cggtgcgttg ctggctgcca ccttccgtgg cctgaccaat gacgtttact tccaggtagg
    15361 cctgctcaca accattgggt tgtcggcgaa gaacgcgata cttatcgtcg aattcgccaa
    15421 agacttgatg gataaagaag gtaaaggtct gattgaagcg acgcttgatg cagtgcggat
    15481 gcgtttacgt ccgatcctga tgacctcgct ggcgtttatc ctcggcgtta tgccgctggt
    15541 tatcagtact ggtgctggtt ccggcgcgca gaacgcagta ggtaccggtg taatgggcgg
    15601 gatggtgacc gcaacggtac tggcaatctt cttcgttccg gtattctttg tggtggttcg
    15661 ccgccgcttt agccgcaaga atgaagatat cgagcacagc catactgtcg atcatcattg
    15721 atacaacgtg taatcactaa ggccgcgcaa gcggcctttt ttatgcataa cctacgaaca
    15781 ttaaggagta attgaaccac caactcagga tctcatacga aaaccagtat taaccacgga
    15841 taaaattca
//
LOCUS       DAZOYL010000070        15833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZOYL010000070 DAZOYL010000000
VERSION     DAZOYL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.070"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            262..666
                     /gene="sdhC"
                     /locus_tag="ACHUOY_001634"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             262..666
                     /gene="sdhC"
                     /locus_tag="ACHUOY_001634"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0682437.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            660..1007
                     /gene="sdhD"
                     /locus_tag="ACHUOY_001635"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             660..1007
                     /gene="sdhD"
                     /locus_tag="ACHUOY_001635"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR0682438.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1007..2773
                     /gene="sdhA"
                     /locus_tag="ACHUOY_001636"
     CDS             1007..2773
                     /gene="sdhA"
                     /locus_tag="ACHUOY_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR0682439.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2789..3505
                     /gene="sdhB"
                     /locus_tag="ACHUOY_001637"
     CDS             2789..3505
                     /gene="sdhB"
                     /locus_tag="ACHUOY_001637"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR0682440.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3806..6607
                     /gene="sucA"
                     /locus_tag="ACHUOY_001638"
     CDS             3806..6607
                     /gene="sucA"
                     /locus_tag="ACHUOY_001638"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR0682441.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6622..7839
                     /gene="odhB"
                     /locus_tag="ACHUOY_001639"
     CDS             6622..7839
                     /gene="odhB"
                     /locus_tag="ACHUOY_001639"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR0682442.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7933..9099
                     /gene="sucC"
                     /locus_tag="ACHUOY_001640"
     CDS             7933..9099
                     /gene="sucC"
                     /locus_tag="ACHUOY_001640"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR0682443.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9099..9968
                     /gene="sucD"
                     /locus_tag="ACHUOY_001641"
     CDS             9099..9968
                     /gene="sucD"
                     /locus_tag="ACHUOY_001641"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR0682444.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10072..10794)
                     /gene="mngR"
                     /locus_tag="ACHUOY_001642"
     CDS             complement(10072..10794)
                     /gene="mngR"
                     /locus_tag="ACHUOY_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR0682445.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            10963..12879
                     /gene="mngA"
                     /locus_tag="ACHUOY_001643"
     CDS             10963..12879
                     /gene="mngA"
                     /locus_tag="ACHUOY_001643"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR0682446.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            12897..15530
                     /gene="mngB"
                     /locus_tag="ACHUOY_001644"
     CDS             12897..15530
                     /gene="mngB"
                     /locus_tag="ACHUOY_001644"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR0682447.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            15555..15782
                     /locus_tag="ACHUOY_001645"
     CDS             15555..15782
                     /locus_tag="ACHUOY_001645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0682448.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
BASE COUNT         3785 a         3963 c         4363 g         3722 t
ORIGIN      
        1 caaactatat gtaggttaat tgtaatgatt ttgtgaacag cctatactgc cgccaggtct
       61 ccggaacacc ctgcaatccc gagccaccca gcgttgtaac gtgtcgtttt cgcatctgga
      121 agcagtgttt tgcatgacgc gcagttatag aaaggacgct gtctgacccg caagcagacc
      181 ggaggaagga aatcccgacg tctccaggta acagaaagtt aacctctgtg cccgtagtcc
      241 ccagggaata ataagaacag catgtgggcg ttattcatga taagaaatgt gaaaaaacaa
      301 agacctgtta atctggacct acagaccatc cggttcccca tcacggcgat agcgtccatt
      361 ctccatcgcg tttccggtgt gatcaccttt gttgcagtgg gcatcctgct gtggcttctg
      421 ggtaccagcc tctcttcccc tgaaggtttc gagcaagctt ccgcgattat gggcagcttc
      481 ttcgtcaaat ttatcatgtg gggcatcctt accgctctgg cgtatcacgt cgtcgtaggt
      541 attcgccaca tgatgatgga ttttggctat ctggaagaaa cattcgaagc gggtaaacgc
      601 tccgccaaaa tctcctttgt tattactgtc gtgctttcac ttctcgcagg agtcctcgta
      661 tggtaagcaa cgcctccgca ttaggacgca atggcgtaca tgatttcatc ctcgttcgcg
      721 ctaccgctat cgtcctgacg ctctacatca tttatatggt cggttttttc gccaccagtg
      781 gcgaactgac atatgaagtc tggatcggtt tcttcgcctc tgcgttcacc aaagtgttca
      841 ccctgctggc gctgttttct atcttgattc atgcctggat cggcatgtgg caggtgttga
      901 ccgactacgt taaaccgctg gcattgcgcc tgatgctgca actggtgatt gtcgttgcac
      961 tggtggttta cgtgatttat ggattcgttg tggtgtgggg tgtgtgatga aattgccagt
     1021 cagagaattt gatgcagttg tgattggtgc cggtggcgca ggtatgcgcg cggcgctgca
     1081 aatttcccag agcggccaga cctgtgcgct gctctctaaa gtcttcccga cccgttccca
     1141 taccgtttct gcgcaaggcg gcattaccgt tgcgctgggt aatacccatg aagataactg
     1201 ggaatggcat atgtacgaca ccgtaaaagg gtcggactat atcggtgacc aggacgcgat
     1261 tgaatatatg tgtaaaaccg ggccggaagc gattctggaa ctcgaacaca tgggcctgcc
     1321 gttctcgcgt cttgatgatg gtcgtatcta tcaacgtccg tttggcggtc agtcgaaaaa
     1381 cttcggcggc gagcaggcgg cacgcactgc ggcggcggct gaccgtaccg gtcacgcact
     1441 gttgcacacg ctttatcagc agaacctgaa aaaccacacc accattttct ccgagtggta
     1501 tgcgctggat ctggtgaaaa accaggatgg cgcggtggtg ggttgtaccg cactgtgcat
     1561 cgaaaccggt gaagtggttt atttcaaagc ccgcgctacc gtgctggcga ctggcggggc
     1621 agggcgtatt tatcagtcca ccactaacgc ccacattaac actggcgacg gtgtcggcat
     1681 ggctatccgc gcaggcgtac cggtgcagga tatggaaatg tggcaattcc acccgaccgg
     1741 cattgccggt gcgggcgtac tggtcaccga aggttgccgt ggtgaaggcg gttatctgct
     1801 gaacaaacat ggcgaacgct ttatggagcg ttatgcgccg aacgccaaag acctggcggg
     1861 ccgtgatgtg gtggcgcgtt ccatcatgat tgaaatccgt gaaggtcgcg gctgtgatgg
     1921 tccgtggggg ccacacgcga aactgaaact cgatcacctg ggtaaagaag ttctcgaatc
     1981 ccgtctgccg ggtatcctgg agctttcccg tacctttgct cacgtcgatc cggtgaaaga
     2041 gccgattccg gttatcccaa cctgtcacta catgatgggc ggtattccga ccaaagtgac
     2101 cggtcaggcg ctgacggtga acgagaaagg cgaagatgtg gttgttccgg ggctgtttgc
     2161 cgttggtgaa atcgcttgtg tatcggtaca cggcgctaac cgtctgggcg gcaactcgct
     2221 gctggacctg gtggtctttg gtcgtgcggc aggtctgcat ctgcaagagt ctatcgccga
     2281 acagggcgca ttgcgcgatg ccagcgagtc tgatgttgaa gcatctctgg atcgtctgca
     2341 ccgctggaac aataatcgta acggtgaaga tccggtggcg attcgtaaag cgctgcaaga
     2401 atgtatgcag cataacttct cggtcttccg tgaaggtgat gcgatggcga aagggcttga
     2461 gcagttgaaa gtgatccgcg agcgtctgaa aaatgcccgt ctggatgaca cttccagcga
     2521 gttcaacacc cagcgcgttg agtgcttgga actggataac ctgatggaaa cggcgtatgc
     2581 aacggctgtt tctgccaact tccgtaccga aagccgtggc gcacatagcc gcttcgactt
     2641 cccggatcgt gatgatgaaa actggctgtg ccactccctg tatctgccag agtcggaatc
     2701 catgacgcgc cgaagcgtca acatggaacc gaaactgcgc ccggcattcc cgccgaagat
     2761 tcgtacttac taatgcggag acaggaaaat gagactcgag ttttcaattt atcgctataa
     2821 cccggatgtt gatgatgctc cgcgtatgca ggattacccc ctggaagcgg aagaaggtcg
     2881 cgacatgatg ctgctggatg cgcttatcca gctgaaagag aaagatccca gcctgtcgtt
     2941 ccgccgctcc tgccgtgaag gtgtgtgcgg ttccgacggt ctgaacatga acggcaagaa
     3001 tggtctggcc tgtattaccc cgatttcggc actcaaccag ccgggcaaga agattgtgat
     3061 tcgcccgctg ccaggtttac cggtgatccg cgatttggtg gtagacatgg gacaattcta
     3121 tgcgcaatat gagaaaatta agccttacct gttgaataat ggacaaaatc cgccagctcg
     3181 cgagcattta cagatgccag agcagcgcga aaaactcgac gggctgtatg aatgtattct
     3241 ctgcgcatgt tgttcaacct cttgtccgtc tttctggtgg aatcccgata agtttatcgg
     3301 cccggcaggc ttgttagcgg catatcgttt cctgatcgat agccgtgata ccgagactga
     3361 cagccgcctc gacggtttga gcgatgcatt cagcgtattc cgctgtcaca gcatcatgaa
     3421 ctgcgtcagt gtatgtccga aggggctgaa cccgacgcgc gccatcggcc atattaagtc
     3481 gatgttgttg caacgtaatg cgtaaaccgt aggcctgata agacgcgcaa gcgtcgcatc
     3541 aggcaaccag tgccggatgc ggcgtgaacg ccttatccgg cctacaagtc attacccgta
     3601 ggcctgataa gcgcagcgca tcaggcgtaa caaagaaatg caggaaatct ttaaaaactg
     3661 cccctgacac taagacagtt tttaaaggtt ccttcgcgag ccactacata gacaagagct
     3721 cgcaagtgaa ccccggcacg cacatcactg tgcgtggtag tatccacggc gaagtaagca
     3781 taaaaaagat gcttaaggga tcacgatgca gaacagcgct ttgaaagcct ggttggactc
     3841 ttcttacctc tctggcgcaa accagagctg gatagaacag ctctatgaag acttcttaac
     3901 cgatcctgac tcggttgacg ctaactggcg ttcgacgttc cagcagttac ctggtacggg
     3961 agtcaaaccg gatcaattcc actctcaaac gcgtgaatat ttccgccgcc tggcgaaaga
     4021 cgcttcacgt tactcttcaa cgatctccga ccctgacacc aatgtgaagc aggttaaagt
     4081 cctgcagctc attaacgcat accgcttccg tggtcaccag catgcgaatc tcgatccgct
     4141 gggactgtgg cagcaagata aagtggccga tctggatccg tctttccacg atctgaccga
     4201 agcagacttc caggagacct tcaacgtcgg ttcatttgcc agcggcaaag aaaacatgaa
     4261 actcggcgag ctgctggaag ccctcaagca aacctactgt ggcccgattg gtgccgagta
     4321 tatgcacatt accagcaccg aagaaaaacg ctggatccaa cagcgtatcg agtctggtcg
     4381 cgcgactttc aatagcgaag agaaaaaacg cttcttaagc gaactgaccg ccgctgaagg
     4441 ccttgaacgt tacctcggcg caaaattccc tggcgcaaaa cgcttctcgc tggaaggcgg
     4501 tgacgcgtta atcccgatgc ttaaagagat gatccgccac gctggcaaca gcggcacccg
     4561 cgaagtggtt cttgggatgg cgcaccgtgg tcgtctgaac gtgctggtga acgtgctggg
     4621 taaaaaaccg caagacttgt tcgacgagtt tgccggtaaa cataaagaac acctcggcac
     4681 gggtgacgtg aaataccaca tgggcttctc gtctgacttc cagaccgatg gcggcctggt
     4741 gcacctggcg ctggcgttta acccgtctca ccttgagatt gtaagcccgg tagttatcgg
     4801 ttctgttcgt gcccgtctgg acagacttga tgagccgagc agcaacaaag tgctgccaat
     4861 caccatccac ggtgacgccg cagtgaccgg gcagggcgtg gttcaggaaa ctctgaacat
     4921 gtcgaaagcg cgtggttatg aagttggcgg tacggtacgt atcgttatca acaaccaggt
     4981 tggtttcacc acctctaatc cactggatgc tcgttctaca ccgtactgta ctgatatcgg
     5041 taagatggtt caggcaccga ttttccacgt taacgcggat gatccggaag ccgttgcctt
     5101 tgtgacccgt ctggcgctcg atttccgtaa cacctttaaa cgtgatgtct tcatcgacct
     5161 ggtgtgctac cgccgtcacg gccacaacga agccgacgag ccgagcgcaa cccagccgct
     5221 gatgtatcag aaaatcaaaa aacatccgac accgcgcaaa atctacgctg acaagctgga
     5281 gcaggaaaaa gtggcgacgc tggaagatgc caccgagatg gttaacctgt accgcgatgc
     5341 gctggatgct ggcgattgcg tagtggcaga gtggcgtccg atgaacatgc actctttcac
     5401 ctggtcgccg tacctcaacc acgaatggga cgaagagtac ccgaacaaag ttgagatgaa
     5461 gcgcctgcag gagctggcga aacgcatcag cacggtgccg gaagcagttg aaatgcagtc
     5521 tcgcgttgcc aagatttatg gcgatcgcca ggcgatggct gccggtgaga aactgttcga
     5581 ctggggcggt gcggaaaacc tcgcttacgc cacgctggtt gacgaaggca ttccggttcg
     5641 cctgtcgggt gaagactccg gtcgcggtac cttcttccac cgccacgcgg tgatccacaa
     5701 ccagtctaac ggttccactt acacgccgtt gcaacacatc cataacgggc agggcgcgtt
     5761 ccgcgtctgg gactccgtac tgtctgaaga agcagtgctg gcgtttgaat acggttatgc
     5821 caccgcagaa ccacgcactc tgaccatctg ggaagcgcag ttcggtgact tcgccaacgg
     5881 tgcgcaggtg gttatcgacc agttcatctc ttctggcgaa cagaaatggg gccggatgtg
     5941 tggtctggtg atgttgctgc cgcacggtta cgaagggcag gggccggagc actcctccgc
     6001 gcgtctggaa cgttatctgc aactttgtgc tgagcaaaac atgcaggttt gcgttccgtc
     6061 taccccggca caggtttacc acatgctgcg tcgtcaggcg ctgcgcggga tgcgtcgtcc
     6121 gctggtagtg atgtcgccga aatccctgct gcgtcatccg ctggcggttt ccagcctcga
     6181 agaactggcg aacggcacct tcctgccagc catcggtgaa atcgacgagc ttgatccgaa
     6241 gggcgtgaag cgcgtagtga tgtgttctgg taaggtttat tacgacctgc tggaacagcg
     6301 tcgtaagaac aatcaacacg atgtcgccat tgtgcgtatc gagcaactct acccgttccc
     6361 gcataaagcg atgcaggaag tgttgcagca gtttgctcac gtcaaggatt ttgtctggtg
     6421 ccaggaagag ccgctcaacc agggcgcatg gtactgcagc cagcatcatt tccgtgaagt
     6481 gattccgttt ggggcttctc tgcgttatgc aggccgcacg gcctccgcct ctccggcggt
     6541 agggtatatg tccgttcacc agaaacagca acaagatctg gttaatgacg cgctgaacgt
     6601 cgaataaata aaggatacac aatgagtagc gtagatattc tggtccctga cctgcctgaa
     6661 tccgtagccg atgccaccgt cgcaacctgg cataaaaaac ccggcgacgc agtcgtacgt
     6721 gatgaagtgc tggtagaaat cgaaactgac aaagtggtac tggaagtacc ggcatcagca
     6781 gacggcattc tggatgcggt tctggaagat gaaggtacaa cggtaacgtc tcgtcagatc
     6841 cttggtcgcc tgcgtgaagg caacagcgcc ggtaaagaaa ccagcgccaa atctgaagag
     6901 aaagcgtcca ctccggcgca acgccagcag gcgtctctgg aagagcaaaa caacgatgcg
     6961 ttaagcccgg cgatccgtcg cctgctggct gaacacaatc tcgacgccag cgccattaaa
     7021 ggcaccggtg tgggtggtcg tctgactcgt gaagatgtgg aaaaacatct ggcgaaagcc
     7081 ccggcgaaag agtctgctcc ggcagcggct gctccggcgg cgcaaccggc tctggctgca
     7141 cgtagtgaaa aacgtgtccc gatgactcgc ctgcgtaagc gtgtggcaga gcgtctgctg
     7201 gaagcgaaaa actccaccgc catgctgacc acgttcaacg aagtcaacat gaagccgatt
     7261 atggatctgc gtaagcagta cggtgaagcg tttgaaaaac gccacggcat ccgtctgggc
     7321 tttatgtcct tctacgtgaa agcggtggtt gaagccctga aacgttaccc ggaagtgaat
     7381 gcttctatcg acggtgatga cgtggtttac cacaactatt tcgacgtcag catggcggtt
     7441 tccacgccgc gcggcctggt gacgccggta ctgcgtgatg tcgataccct cggcatggca
     7501 gacatcgaga agaaaatcaa agagctggca gttaaaggcc gtgacggcaa gctgacggtt
     7561 gaagatctga ccggtggtaa cttcaccatc accaacggtg gtgtgttcgg ttccctgatg
     7621 tctacgccga tcatcaaccc gccgcagagc gcaattctgg gtatgcacgc tatcaaagat
     7681 cgtccgatgg cggtgaatgg tcaggttgag atcctgccga tgatgtacct ggcgctgtcc
     7741 tacgatcacc gtctgatcga tggtcgcgaa tccgtgggct tcctggtaac gatcaaagag
     7801 ttgctggaag atccgacgcg tctgctgctg gacgtgtagt agtttaagct tcacctgcac
     7861 tgtagaccga cgtggatagg gtgtcggtct acggtttaaa agataacgat tactgaagga
     7921 tggacagaac acatgaactt acatgaatat caggcaaaac aactttttgc ccgctatggc
     7981 ttaccagcac cggtgggtta tgcctgtact actccgcgcg aagcagaaga agccgcttca
     8041 aaaatcggtg ccggcccgtg ggtagtgaaa tgtcaggttc acgctggtgg ccgcggtaaa
     8101 gcgggcggtg tgaaagttgt aaacagcaaa gaagacatcc gtgcttttgc agaaaactgg
     8161 ctcggcaagc gtctggtaac gtatcaaaca gatgccaatg gccaaccggt taaccagatt
     8221 ctggttgaag cagcgaccga tatcgctaaa gagctgtatc tcggtgccgt tgtagaccgt
     8281 agttcccgtc gtgtggtctt tatggcctcc accgaaggcg gcgtggaaat cgaaaaagtg
     8341 gcggaagaaa ctccgcacct gatccacaaa gttgcgcttg atccgctgac tggcccgatg
     8401 ccgtatcagg gacgcgagct ggcgttcaaa ctgggtctgg aaggtaaact ggttcagcag
     8461 ttcaccaaaa tcttcatggg cctggcgacc attttcctgg agcgcgacct ggcgttgatc
     8521 gaaatcaacc cgctggtcat caccaaacag ggcgatctga tttgcctcga cggcaaactg
     8581 ggcgctgacg gcaacgcact gttccgccag cctgatctgc gcgaaatgcg tgaccagtcg
     8641 caggaagatc cgcgtgaagc acaggctgca cagtgggaac tgaactacgt tgcgctggac
     8701 ggtaacatcg gttgtatggt taacggcgca ggtctggcga tgggtacgat ggacatcgtt
     8761 aaactgcacg gcggcgaacc ggctaacttc cttgacgttg gcggcggcgc aaccaaagaa
     8821 cgtgtaaccg aagcgttcaa aatcatcctc tctgacgaca aagtgaaagc cgttctggtt
     8881 aacatcttcg gcggtatcgt tcgttgcgac ctgatcgctg acggtatcat cggcgcggta
     8941 gcagaagtgg gtgttaacgt accggtcgtg gtacgtctgg aaggtaacaa cgccgaactc
     9001 ggcgcgaaga aactggctga cagcggcctg aatattattg cagcaaaagg tctgacggat
     9061 gcagctcagc aggttgttgc cgcagtggag gggaaataat gtccatttta attgataaaa
     9121 acaccaaggt tatctgccag ggctttactg gtagccaggg gactttccac tcagaacagg
     9181 ccattgcata cggcactaaa atggttggcg gcgtaacccc aggtaaaggc ggtactaccc
     9241 acctcggcct gccggtgttc aacaccgtgc gtgaagctgt tgctgctact ggcgctaccg
     9301 cttctgttat ctacgtacca gcaccgttct gcaaagactc cattctggaa gctattgatg
     9361 caggcatcaa actgattatc accatcactg aaggcatccc gacgctggat atgctgactg
     9421 tgaaagtgaa actggacgaa gccggcgttc gtatgatcgg cccgaactgc ccgggcgtta
     9481 tcactccggg tgaatgcaaa atcggtatcc agccgggtca cattcacaaa ccgggtaaag
     9541 tgggtatcgt ttcccgttcc ggtacactga cctatgaagc ggttaaacag accacggatt
     9601 acggtttcgg tcagtcgacc tgtgttggta tcggcggtga cccgatccca ggctctaact
     9661 ttatcgacat cctcgaaatg ttcgaaaaag atccgcagac cgaagcgatc gtgatgatcg
     9721 gtgagatcgg cggtagcgct gaagaagaag cggcagcgta catcaaagag cacgttacca
     9781 agccagttgt aggttacatc gctggtgtga ctgcgccgaa aggcaaacgt atgggccacg
     9841 cgggtgcgat cattgctggt gggaaaggga ctgcggatga gaaattcgct gctctggaag
     9901 ccgcaggcgt gaaaaccgtt cgcagcctgg cggatatcgg tgaagcactg aaaactgttc
     9961 tgaaataaat atctgtaata agaaatagcc ctcgccgctt ccctctacag gaatggcgaa
    10021 gggctgtcgg tttcgacatg gttggccatc gtatgatggc cttttttgtg cttatcgcga
    10081 tgattttcgc tgcgctatca gggtaaattt atagtcatcg gtattaaaag cgttgcggct
    10141 atattcaaac acccgaccat caactaaata tccacgcgat actttttcaa gaatcggctt
    10201 tgtctggctg atattaagca gacggctcat ctcttcggtt ggcatcagag gaatgatttc
    10261 ctgttcgcta cgatcgataa ccatcttctt cacttcttca ataaagtgat atttcgaatt
    10321 ttccatgacc tgccaggtga gatccgggaa caacgcaagc ggcatccagg tttcttccag
    10381 cgccattggc ttttgcttgc gatagcgcac gcgcttcaca tgccacacac gatcctgcgc
    10441 ggtgatttgt agctgttgct gaagaaaatc gtcagccgga atcacttcaa atatcaggat
    10501 ttcactgtgt gtatcgacgt gacggtccga cagtttttca tcaaaactgg ttaactgaaa
    10561 aatatcgtaa ttgacccgct cttctttgac gtaagtcccg ctgccctgaa tgctttcgag
    10621 gatctgctgc tcgactagct ggcgcaaagc ctgacgcacc gtaacccggc tgacgccaaa
    10681 ctctgtttgt agcgctgatt cagtgggtaa cgcatcgcca ggtttaagct cgccacgcgc
    10741 aatttgttca cgaatgcgat cggcaatctg ccggtataag ggcttgtgtc ccatttttag
    10801 tatctcatta atatgaattt agccattatg cccgataaat tcatcctgta aataatacaa
    10861 atacaataca aataatttca atcaagtgaa attgatcaca tgatggtatt gttttatcgg
    10921 gcacactggc gcgactataa aaacgatcaa gtgaggatca tgatgaatct gacgactctg
    10981 acccaccgcg atgcgttgtg tctgaatgcg cgctttacca gccgtgaaga ggccatccac
    11041 gcgttgactc aacgtcttgc tgctctgggg aaaatttcca gtactgagca atttctggaa
    11101 gaagtgtatc gccgtgaaag ccttggcccg accgccttag gtgaagggtt ggctgtgccg
    11161 catggcaaaa ctgctgcggt aaaagaagcg gcgtttgcgg tcgccacact cagcgagccg
    11221 cttcagtggg aaggcgttga tggcccggaa gcagttgatt tagtggtgct gctggcgatt
    11281 ccccccaatg aagcgggtac tacgcatatg caactgctga cagcgctgac cacgcgcctt
    11341 gcggatgatg agattcgggc gcgtatacag tcggcgacga cgcctgatga gttgctctcg
    11401 gcgctggatg acaagggagg cacgcaacct tctgcctctt tttccaacgc gccaactatc
    11461 gtctgcgtaa cggcctgtcc ggcgggtatt gctcacacct atatggctgc ggaatatctg
    11521 gaaaaagccg gacgcaaact cggcgtaaat gtttacgttg aaaaacaagg cgctaacggc
    11581 attgaagggc gtttaacggc ggatcaactc aatagtgcaa ccgcctgtat ttttgcggct
    11641 gaagtcgcca tcaaggagag tgagcgtttt aatggcattc ccgcgctttc agtgcctgtt
    11701 gccgagccga ttcgccatgc agaagcgttg atccaacaag cgcttaccct caagcgtagc
    11761 gatgagacgc gtaccgtaca gcaagatacg caaccggtga aaagtgtcaa aacggagctg
    11821 aaacaggcac tgttgagcgg aatctctttt gccgtaccgt tgattgtcgc ggggggcacg
    11881 gtgctggcgg tcgcggtatt actgtcgcaa atcttcgggc tacaagatct gtttaatgaa
    11941 gaaaactcct ggctgtggat gtaccgcaag ctgggcggcg ggctgctcgg aattttgatg
    12001 gtaccggtgc tcgcggccta taccacctat tctctggcag ataaaccggc gttagcgcca
    12061 ggctttgcgg ctggacttgc cgccaacatg atcggctccg ggtttctcgg cgcggtcgtt
    12121 ggcggattga tagccggtta cttgatgcgc tgggtgaaaa atcacttgcg tcttagcagt
    12181 aaattcaatg gattcctgac tttttatctc tacccggtgc tcggtacgtt gggagcgggc
    12241 agtctgatgc tgtttgtggt gggagaacct gtcgcatgga tcaataactc gcttaccgcc
    12301 tggctgaacg gtctgtcagg aagtaacgcg ctgttgctgg gtgccattct cggttttatg
    12361 tgttcctttg accttggagg gccagtgaac aaagccgctt atgcattctg cctgggcgca
    12421 atggcgaacg gcgtttacgg cccgtatgcc attttcgcct ccgtcaaaat ggtttcggca
    12481 tttaccgtaa ccgcttccac gatgctcgca ccgcgcctgt ttaaagagtt tgaaattgag
    12541 accgggaaat ccacctggct gttagggctg gcaggtatta ccgaaggggc gatcccgatg
    12601 gcgattgaag atccgctgcg ggttattggt tcgtttgtgc tgggctctat ggtaacgggc
    12661 gctattgtcg gtgcgatgaa tatcggcctt tcgacacccg gtgccggcat tttctcgctc
    12721 tttttacttc atgataatgg cgcgggcggt gttatggcgg caattggctg gtttggtgcg
    12781 gcattggtgg gggctgcaat ctcgactgca attctcctga tctggcggcg tcacgcggtt
    12841 aagcatggca actatctgac tgatggcgta atgccataaa caaaaaggaa acgacgatga
    12901 aagcggtatc tcgcgttcac atcaccccgc atatgcattg ggatcgggag tggtatttca
    12961 ccaccgaaga gtcacgcatt ctgctggtca ataatatgga agagatcctg tgccgactgg
    13021 aacaggacaa cgaatacaaa tattacgtac tcgacgggca aacggcgatc ctcgaagatt
    13081 atttcgcggt gaaaccggaa aacaaagacc gcgtgaagaa acaggtagaa gccggtaagt
    13141 tgattatcgg cccctggtat acccagaccg ataccacgat tgtttctgcg gaatccatcg
    13201 tccgtaatct gatgtacgga atgcgtgact gcctcgcgtt tggcgagccg atgaaaatag
    13261 gttatttacc ggattccttt ggcatgtccg ggcaactgcc gcatatttac aatggatttg
    13321 gcattacccg cactatgttc tggcgcggat gttcggagcg ccacggtact gataaaaccg
    13381 agtttttgtg gcaaagcagt gacggtagcg aagtgacggc gcaggtgctg ccgctgggct
    13441 acgccatcgg taagtactta cctgccgacg aaaacggatt acgtaaacgc ctcgacagtt
    13501 attttgacgt gctggaaaaa gcgtctgtaa ccaaagagat tttgctgccg aatggtcatg
    13561 accagatgcc attgcagcaa aatatcttcg aagtgatgga taagctacgt gagatctacc
    13621 ctcaacgtaa gtttgtgatg agccgctttg aagaggtatt tgagaagatc gaagcgcagc
    13681 gtgaaagtct ggccaccctg aaaggtgaat ttatcgatgg caaatatatg cgcgtacatc
    13741 gcaccatcgg ttccacgcgc atggatatca aaattgccca cgcacgtatt gaaaataaga
    13801 ttgttaatct gctggaaccg ctggcaacgc tggcctggac gttgggtttt gaataccacc
    13861 acggcttgct ggagaaaatg tggaaagaga tcttaaaaaa tcatgcccac gacagtatcg
    13921 gttgttgttg cagtgacaaa gttcatcgcg aaattgtcgc ccgcttcgaa ctggctgaag
    13981 acatggcgga taatctgctg cgtttctaca tgcgcaaaat tgccgataac atgccacaga
    14041 gtgatgccga caaactcgtg ctgtttaacc tgatgccctg gccgcgggaa gaagttatta
    14101 acaccactgt gcggctgcgc gccagccagt ttaatctaca agacgatcgc ggtcagcctg
    14161 taccgtattt tattcgccat gcccgtgaga tcgatcctgg cctaatcgat cggcaaattg
    14221 ttcattatgg caattatgat ccctttatgg agtttgatat tcagatcaac cagattgtcc
    14281 cttctatggg ctatcgcacg ctttatattg aagcgaatca gcctggcaac gtgattgcgg
    14341 caaaaagtga cgctgaaggg attctggaaa atgctttctg gcagattgcg ctcaatgagg
    14401 atggttctct gcaactggta gataaagaca gcggtgtgcg ctatgaccgg gtattgcaaa
    14461 ttgaagaaag ctctgatgat ggtgatgaat atgactattc acccgcaaaa gaagagtggg
    14521 taattaccgc agcgaacgcg aaaccgcaat gcgatattat tcatgaagcc tggcagagca
    14581 gggctgttat ccgctatgac atggcagtgc cgctcaattt gtcagaacgc agcgcccggc
    14641 aatccactgg cagagtaggg ggggtgttgg ttgtcactct tagtcataac agcaggcgta
    14701 ttgatgtgga tatcaacctt gataaccagg ctgacgatca tcgccttcgt gtcctggtcc
    14761 ctacaccttt taacactgac agtgttctgg cagatacgca gtttggttcg ctaacgcgcc
    14821 ccgtgaacga cagtgcaatg aacaactggc agcaagaagg ctggaaagaa gcgccggttc
    14881 cggtatggaa tatgctcaac tatgttgcct tacaggaagg gcgtaacggc atggctgtct
    14941 ttagcgaagg gttacgtgaa tttgaagtca tcggtgaaga gaagaaaacc tttgccatta
    15001 cgttgctgcg tggcgtgggc ttactgggca aagaagatct gcttttaagg cctgggcggc
    15061 cttcgggaat taaaatgcca gtcccggact cacaactacg tggtctgctt tcttgtcgcc
    15121 taagtttatt gagttatacc ggtacgccaa ccgccgctgg tgtagctcag caggcgcgag
    15181 catggctgac tccagtacag tgttacaaca aaatcccatg ggatgcgatg aagctcaaca
    15241 aagccggatt caacgtgccg gaaagttata gtttgttgaa aatgccccca gtgggatgcc
    15301 tgataagcgc acttaagaaa gctgaagacc gacaagaagt gattttacgg ctgtttaatc
    15361 cggctgaatc agcaacctgt gatgcgactg ttgctttcag tcgcgaggtg atttcttgct
    15421 cagaaacgat gatggatgaa cacattacca ccgaggaaaa tcaaggttca aatctatcgg
    15481 ggcctttttt acccggccag tcacggacgt tcagttaccg gcttgcctga atagcaatca
    15541 aaccgaagcc acatatgcgc ggccagattg ttgacaaagg gcgctttgtt catgccggat
    15601 acggcatgaa cgctttattc ggtctacaaa agcaggcaaa ttcaatatat tgcagagatg
    15661 atgtagacac tggcaagcgt agcgcatcag gtaattttgc gtttatcttc actctcaagc
    15721 cacgtatatg tggctttatt tttaacaaaa taataaccct gggtgagtta attataatat
    15781 aattacaagt taattaaatg ttaatatcgg cggggtggat ttatgccttt att
//
LOCUS       DAZOYL010000071        15701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZOYL010000071 DAZOYL010000000
VERSION     DAZOYL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.071"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            12..514
                     /locus_tag="ACHUOY_001646"
                     /pseudo
     CDS             12..514
                     /locus_tag="ACHUOY_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHUOY_001647"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHUOY_001647"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR0682449.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHUOY_001648"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHUOY_001648"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR0682450.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHUOY_001649"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHUOY_001649"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR0682451.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHUOY_001650"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHUOY_001650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR0682452.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHUOY_001651"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHUOY_001651"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR0682453.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHUOY_001652"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHUOY_001652"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR0682454.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHUOY_001653"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHUOY_001653"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR0682455.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHUOY_001654"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHUOY_001654"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR0682456.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHUOY_001655"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHUOY_001655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR0682457.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHUOY_001656"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHUOY_001656"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR0682458.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHUOY_001657"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHUOY_001657"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR0682459.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHUOY_001658"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHUOY_001658"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR0682460.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHUOY_001659"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHUOY_001659"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR0682461.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT         3848 a         4488 c         4170 g         3195 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat c
//
LOCUS       DAZOYL010000072        15582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZOYL010000072 DAZOYL010000000
VERSION     DAZOYL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.072"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..2065
                     /locus_tag="ACHUOY_001660"
     CDS             <1..2065
                     /locus_tag="ACHUOY_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR0682462.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2081..3115
                     /locus_tag="ACHUOY_001661"
     CDS             2081..3115
                     /locus_tag="ACHUOY_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR0682463.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="ACHUOY_001662"
     CDS             complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="ACHUOY_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR0682464.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="ACHUOY_001663"
     CDS             complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="ACHUOY_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR0682465.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4699..4971
                     /gene="rcnR"
                     /locus_tag="ACHUOY_001664"
                     /pseudo
     CDS             4699..4971
                     /gene="rcnR"
                     /locus_tag="ACHUOY_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5063..5159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5194..5982
                     /gene="thiM"
                     /locus_tag="ACHUOY_001665"
     CDS             5194..5982
                     /gene="thiM"
                     /locus_tag="ACHUOY_001665"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR0682466.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5979..6779
                     /gene="thiD"
                     /locus_tag="ACHUOY_001666"
     CDS             5979..6779
                     /gene="thiD"
                     /locus_tag="ACHUOY_001666"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR0682467.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6844..7662
                     /locus_tag="ACHUOY_001667"
     CDS             6844..7662
                     /locus_tag="ACHUOY_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR0682468.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7714..8460
                     /locus_tag="ACHUOY_001668"
     CDS             7714..8460
                     /locus_tag="ACHUOY_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0682469.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8434..9399)
                     /locus_tag="ACHUOY_001669"
     CDS             complement(8434..9399)
                     /locus_tag="ACHUOY_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR0682470.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9396..10400)
                     /locus_tag="ACHUOY_001670"
     CDS             complement(9396..10400)
                     /locus_tag="ACHUOY_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR0682471.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10397..11674)
                     /locus_tag="ACHUOY_001671"
     CDS             complement(10397..11674)
                     /locus_tag="ACHUOY_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR0682472.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11931..12983
                     /gene="fbaB"
                     /locus_tag="ACHUOY_001672"
     CDS             11931..12983
                     /gene="fbaB"
                     /locus_tag="ACHUOY_001672"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR0682473.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13302..14156
                     /gene="gatY"
                     /locus_tag="ACHUOY_001673"
     CDS             13302..14156
                     /gene="gatY"
                     /locus_tag="ACHUOY_001673"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR0682474.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14185..15442
                     /gene="gatZ"
                     /locus_tag="ACHUOY_001674"
                     /pseudo
     CDS             14185..15442
                     /gene="gatZ"
                     /locus_tag="ACHUOY_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15452..>15582
                     /locus_tag="ACHUOY_001675"
     CDS             15452..>15582
                     /locus_tag="ACHUOY_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0682475.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3727 c         3987 g         3780 t
ORIGIN      
        1 tggctatgtt ccaccggaaa actgggagcg gggtattaat gcgttttata cctcttatta
       61 tgtgagtcag tattacagtg actataaagc gtcgggtaat agtaagagta catatgtacg
      121 ttttaacagc gggttaaatt tactggggtg gcaactgcat tctgatgcca gtttcagtaa
      181 aacaaataac aatccagggg tgtggaaaag caataccctg tatctggaac gtggatttgc
      241 ccaacttctc ggcacgcttc gcgtgggtga tatgtacaca tcaagcgata tttttgattc
      301 tgttcgcttc agaggtgtgc ggttgtttcg tgatatgcag atgttgccta actcgaaaca
      361 aaattttacg ccacgggtgc aggggattgc tcagagtaac gcgctggtaa ctattgaaca
      421 gaatggtttt gtggtttatc agaaagaggt tcctcctggc ccgtttgcga ttacagattt
      481 gcagttggcc ggtggtggag cagatcttga tgtcagcgtg aaagaggcgg acggttcggt
      541 aaccacctat ctggtgcctt atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa
      601 atatgatttt gcggcgggtc gtagccatat tgaaggggcg agcaaacata gtgattttgt
      661 ccaggcgggt tatcagtatg gttttaataa cttattgacg ttgtatggtg gctcgatggt
      721 tgcgaataat tattacgcgt tcactttggg gactggctgg aatacacgca ttggtgccat
      781 ttccgtcgat gccacgaagt cgcatagtaa acaagacaac ggcgatgtgt ttgacgggca
      841 aagttatcaa attgcctaca acaaatttgt gagccaaacg tcgacgcgtt ttggtctggc
      901 ggcctggcgt tattcgtcgc gtgattaccg gacatttaac gatcacgttt gggcaaacaa
      961 taaagataat tatcgccgtg atgaaaacga tgtctatgac attgccgatt attaccagaa
     1021 tgattttggt cgcaaaaata gcttttccgc caatatgagc cagtcattgc cagaaggttg
     1081 ggggtctgtg tcattaagta cgttatggcg agattactgg gggcgtagcg gcagtagtaa
     1141 ggattatcag ttgagttatt ccaacaacct gcgacggata agctataccc tcgcggcaag
     1201 ccaggcttat gacgagaatc atcatgaaga gaaacgtttt aatattttta tatcgattcc
     1261 ctttgattgg ggtgatgacg tttcgacgcc tcgtcggcaa atatatatgt ctaactcaac
     1321 gacgtttgat gatcaggggt ttgcctcaaa taatacggga ttatcaggaa cagtagggag
     1381 tcgggatcag ttcaattatg gtgtcaacct gagtcatcaa catcagggaa atgaaacgac
     1441 agctggagcg aatttaacct ggaacgcgcc ggtggcaaca gtgaatggca gttatagtca
     1501 gtcgagtact tatcgacagg ctggagccag tgtttcaggg ggcattgtcg cctggtcggg
     1561 tggcgttaat ctggcgaacc gtctttccga aacgtttgct gtgatgaatg cgccaggaat
     1621 taaagatgct tatgtcaatg ggcaaaaata tcgcacaaca aaccgtaatg gagtggtggt
     1681 atacgacgga atgacacctt atcgggaaaa tcacctgatg ctggatgtgt cgcaaagcga
     1741 tagcgaagca gaattacgtg gcaaccggaa aattgccgca ccttatcgcg gcgcggttgt
     1801 actggttaat tttgataccg atcagcgcaa gccctggttt ataaaagcgt taagagcgga
     1861 tgggcaacca ttaacgtttg gttatgaagt caatgatatc cacggtcata atattggcgt
     1921 tgtcggccag ggaagccagt tatttattcg caccaatgaa ataccgccat cggttaatgt
     1981 agcaattgat aagcaacaag gactttcatg cacaatcacc ttcggtaaag agattgatga
     2041 aagtagaaat tatatttgcc agtaatagtc aggtggttct atggaaattc gcataatgct
     2101 atttatatta atgatgatgg ttatgcctgt gagctatgcg gcatgttata gtgagttatc
     2161 tgttcagcac aatttggttg ttcaggggga ttttgcactt actcaaacac aaatagcgac
     2221 atatgagcat aattttaatg attcgtcatg cgtgagtaca aatactatca cccctatgag
     2281 ttcgtcggat attattgttg ggctttataa cgataccata aaattaaatt tacattttga
     2341 atggaccaat aaaaacaaca tcacgttgtc aaataatcag accagtttca ccagtggtta
     2401 ttcagttacg gtgacacctg cggccagtaa tgcaaaagtg aatgtttctg cggggggcgg
     2461 cggttcagtg atgattaatg gtgttgcgac attatccagt gcttcatcat cgacacgcgg
     2521 gagtgccgca gtacaatttc tactgtgttt attaggtggc aagtcatggg atgcatgtgt
     2581 aaatagctac agaaatgcat tggcacaaaa tgcaggtgtc tattccttta atctgacatt
     2641 gtcatacaac ccgataacca caacctgcaa accggacgat ttattaatta ctttagacag
     2701 tattcccgtt tcacaattac cagccacagg taacaaagca acaataaata gtaaacaagg
     2761 ggatattatt ctgcgttgta aaaatttatt aggtcaacaa aatcaaacat cacggaaaat
     2821 gcaggtgtat ttatcaagtt ctgatttgtt agccggcagt aacacgatcc tgaaaggtgc
     2881 agaaaataat ggagtcggat ttatgcttga aagcaatggt tctccagtca catttttaaa
     2941 tatcactgat aacagcaaag gatacacaaa tttaaaagaa attgcggcaa agtcaaaact
     3001 tacagataca acggtttcaa ttccgataac tgccagttac tacgtctacg atacaaacaa
     3061 agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg actaataaca
     3121 agcaagacga gcaagtggct aataataaaa aataacaagg tgtctgcatt tccccctcat
     3181 gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt
     3241 taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg
     3301 catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca tcggtgcggt
     3361 acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg aaactactta
     3421 actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg
     3481 cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg gtcataaact
     3541 ctccactttg gggatctgta atatcagtcg catatctttc ctgccataca atataactga
     3601 caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgccc
     3661 taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga atcatcctgg
     3721 gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca tgaagccatg
     3781 tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg gggcgcgttt
     3841 agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga ttgctgcgcc
     3901 aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga ccagtgttgc
     3961 gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg ctgccgggca
     4021 ggggataagg ccaccggtta agccaaatag caaaatttgc cagttggtga cctctctacc
     4081 atcaaagcgt cgtttaatgt cattggcatg ggctcgtgca tgggcatcct gatactcgcc
     4141 atgttcgtga tggtgatgat gtccatggtc gtggtgatct tcgtgatcgt gatgatgatg
     4201 ctcataatca tactcgtgca tattctccag ccagttgcgt tcgccgcgcc aggtacgcca
     4261 gaacatccag aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc
     4321 tgctgattga gcagtaaagc gcttgctgat caccatcccg ccaaaggcaa ttaaccagac
     4381 cactgcggta tgcgaaatag ttgctgccag tccgagcatc accgcttgtt taatggtgcc
     4441 tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg
     4501 aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag
     4561 agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct gattctactc
     4621 cccccagtac ctgatactac cccccagtag attaatagtg ctatgatatt ttcatattct
     4681 tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact gaaagcgcgt
     4741 gccagtaaga ttcagggcta ggtcgtggcg ctcaagaaaa tgctcgacga gccgcatgaa
     4801 tgcgctgcag ttttacaaca gattactgct atccgtggcg cggtaaacgg tctgatgcgg
     4861 gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga gctaaaacgt
     4921 gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata atttattaac
     4981 gcgattgtaa aactgccgtt tttcctcgtt tacaacgcgt gcgctggaca ttaccatcct
     5041 cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag
     5101 aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg accaccaggt
     5161 cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct gggttcagcg
     5221 caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca ctgcatgacc
     5281 aatgatgtgg tgcaaacctt taccgctaat accttgctgg cgctcggtgc atcgccagcg
     5341 atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc cttgttgatt
     5401 aacgttggca cactgacgca gccacgcgct caggcgatgc gtgctgccgt tgagcaagca
     5461 aaaagctctc aaacaccctg gacgcttgat ccagtagcgg ttggtgcgct cgattatcgc
     5521 cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg taatgcttcg
     5581 gaaatcatgg cattagctgg cattgctaat ggtggacggg gagtggatac cactgacgcc
     5641 gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg
     5701 gtcactggcg aggtggatta tgttaccgat ggccatcgcg ccgttggcat tcacggtggc
     5761 gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc
     5821 tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa
     5881 caagccggag agcgcgcagt cgccagaagc gaggggctag gcagttttgt tccacatttc
     5941 cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt aacgctctga
     6001 cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt aaaaccttct
     6061 cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa aatacccgtg
     6121 gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc gattcggtgt
     6181 tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc gatattgttg
     6241 aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc gacaccgtta
     6301 tgctggcaaa aagcggcgac ccactgcttt caccttcggc agttgccacg ctacgcagtc
     6361 gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagcagcc gccttgctcg
     6421 acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg ctgttggcga
     6481 tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa agcccggact
     6541 ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg accaaaaaca
     6601 cccacggtac tggttgtaca ctctctgcgg cgttggctgc actacgcccg cgccatacaa
     6661 actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta gcccaggccg
     6721 acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc tggtggtgag
     6781 caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc agggatgaaa
     6841 ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg cgctcttggg
     6901 ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta ttcgacagca
     6961 gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc tttcgctatt
     7021 cgtattcctg ccagttacac cattcacgga atagatgttt cgcgctggca ggagcggatc
     7081 gactggcagc gtgtggcaaa aatgcgcgat aacggtattc gcttacaatt tgcctttatt
     7141 aaggcgacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg gcaactaagc
     7201 cgcgaaaatg gcttgctgcg cggggcatat cattatttct ccccgtcggt agcagcaccg
     7261 gttcaggtgc gattatttct gcaaacggta gatttctcac aaggcgattt ccctgcggta
     7321 ctggacgtag aagagcgagg aaaattatcg gcaaaagaat tacgcaagag ggtaagtcag
     7381 tggctaaaaa tggtcgaaaa aagtacgggg aaaaagccga ttatttactc aggagccgtt
     7441 ttttatcaca cgaatctggc gggctatttc aatgaatatc cgtggtgggt ggctcactac
     7501 tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca ttccgaccgt
     7561 ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg cacggtggag
     7621 gagttgcagg gattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata
     7681 ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt
     7741 gcccaactga acgaacgtat tttagcggcg gataacacgc cgctttatat taagtttgcc
     7801 gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt
     7861 gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag
     7921 gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac
     7981 atcttcgaat actcgttgaa agaagcgcga ggtttttctc agcaggtggt gttgcgcggg
     8041 aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga aatgtcccga agaagtcgcg
     8101 cagcagctgg cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat tcgctatgtc
     8161 gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt
     8221 gatgccatcg ggatttcact ttatgactat ttccgcagcc agcacattta cccacaacgt
     8281 acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta
     8341 gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct
     8401 attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag
     8461 ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc
     8521 attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc
     8581 acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat
     8641 gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa
     8701 cggcgcggca aatttctcct gccattgctc gccaagtgtt gttatctctg cggataaagc
     8761 aaaacgttcg gcggcaatct cagcctcttg acgattgagc gacactaaag gtcgacaggc
     8821 catgatccgc gccagtaatg catccgggat atcgccaata cgtgggccaa aatcgataaa
     8881 cggcgtcacg tcttgcagct cttccagcca ttccactaac aattcgccgc agggcgaggc
     8941 cagttgataa ccggaaaaat agagcaggct gccaggcgca acggttaatc gcgccagcca
     9001 ctggcgattc cactgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg
     9061 ctcaaccagc gccagacacc agccgttatc accttcggcg ttatcgatca ggctgattaa
     9121 gccctctttt gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa
     9181 ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc
     9241 gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata
     9301 agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt
     9361 taattcaggc agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga
     9421 tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagttcc
     9481 gcctttaacg caggatcgat agcgttaacg ccatgcaacg cgccgcaaat tgccgtcgcc
     9541 atagcaccga tggtgtctgt gtcgccgcca aggttagcgc acaggacggc gcagcgattc
     9601 gggtcggttt gtgctagttc aaccagcgca atggcgcacg gaacggactc aatagtgctg
     9661 gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcattg
     9721 cgcacaattt tcagcgcaat ctccagccgc gccgccagtg aggcgctgaa cgtagtgatg
     9781 cgtttttgct gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc tgaccagctt
     9841 tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga
     9901 tcggatttat gggtcgggct ggacgccagc gctacatcat caataaagga atcaacatca
     9961 cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggtcacgccg
    10021 ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag
    10081 gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc gagaatatta
    10141 cgcccaatca gatccggatc gatcttgcct tcacgttcca gtaacgcatc cgccagacac
    10201 agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc cgcgttattc
    10261 tcctttggtc caggaagaaa acggtcaatc cagccaaagt gtgctttaac gcggctgcgt
    10321 ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata aagagcaccg
    10381 agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg cgatcatcga
    10441 ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac agcacggcga
    10501 taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa gtcagtccgt
    10561 ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga cgatagccga
    10621 gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg gtacgcatat
    10681 gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa tcgtaactta
    10741 cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaatattca tccgtactac
    10801 cgtaaataaa gaagccatag cggatcgcag cggtgaccag accaagcaat aatacctttt
    10861 tgataccaaa gcgtttagtg aaaaacggca atgccagcat aaagaagatt tcagagaact
    10921 ggccgagcgt catccagccg gtggcgtttt tcatgccaac ttcggtcaga taaccgttgg
    10981 caaagatgta atagaacgcc agtggcatcg caaacaggaa tgaacagaag aaaaagacga
    11041 ggaagttttt atcgcgcagc aggatcagcg catccaggcc gagcatgact ttaatatcca
    11101 ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa cgcaaacaca ccgagcagag
    11161 cagaacttcc ggcggtaatc agcagcggga tgttagtcgg tgagatatcg gcatacccca
    11221 gtatttgcgg caagaaacca catgccagac cggaggcaat ccagccgata gtgcccatca
    11281 cacgaatgcg cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg atgctgttag
    11341 tcagcgcaat ggtcggcata taggttagag agtaagccag cagtaacgga aagaacccgg
    11401 caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac atcaataccg
    11461 ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga attggcgaca
    11521 ggatcgccgc aatggcggta caggcatacg accagccaat ttctccggca ctaaaaccgc
    11581 ttttacttaa ccacaaccac aatggcacaa accacgcgcc ccagataaac cattcaacaa
    11641 acatcatgaa cgacagcttt gctgttgttt tcatctttta attccttcat gacaggtaat
    11701 gtaaggtacg cgatgacaat accacttaac aatacctttt aaataccttt gcggtgaaaa
    11761 cttgaccgcg ctaacatttt ttctgatgaa tcgagccaac agaaaacgct gaaaaaacat
    11821 ccaaaagatg gaaaaactcg cccgtaacga ccaggattat gattgcccgc aaaaggcggg
    11881 ccaggacaat ccatagccga tatccaatcg gaatttacgg gagcatagta atgacagata
    11941 ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt atgacaattc
    12001 cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg attgacaata
    12061 atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg cgtctggctg
    12121 gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct gccggagctt
    12181 catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg gcgatcgaag
    12241 cgggctgtaa ctgtgtggcg tcaacttacg gcgtgttggc gtcggtatcg cggcgctatg
    12301 cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt tacccgaata
    12361 cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc gcggtggcgg
    12421 ttggtacgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa gaaatttctg
    12481 cggcctttga acgcgcgcac gagctgggca tggtgaccgt gctgtgggcc tatttgcgaa
    12541 actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg accggtcagg
    12601 caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg gcggaaaata
    12661 acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttat agcaaattga
    12721 ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat atgggccggg
    12781 ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagt gatgcagtgc
    12841 gtactgcggt tatcaacaaa cgcgctggcg gaatggggct gattcttgga cgtaaagcgt
    12901 tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac gtttatctcg
    12961 atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc gtaggacgga
    13021 taaggcgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag cgtcttatca
    13081 tgcctacata gcactgccac gtatgtcgga aaaggcgttt acaccgcatc cggcataaaa
    13141 acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc
    13201 gaacagtttt catattgtcg ttttgtgatc gttatctcga catttaaaaa caaatcattt
    13261 cattatattt tgaaatcgaa aacaagcaac aggatatgaa aatgtacgtg gtatcgacaa
    13321 agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc aatattcaca
    13381 atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca
    13441 tcgccggaac gcctggcaca ttcacttatg ctggtacaga aaatctgttg gcgctggtca
    13501 gtgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat cacacgaaat
    13561 ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc
    13621 atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt tgccatcgct
    13681 ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat gatgtgcaag
    13741 tcaatgaagc cgatgttttt tataccaacc ccgctcaggc gcgtgaattt gccgaggcaa
    13801 ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat gccagcgcac
    13861 cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc
    13921 tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa ctggggatat
    13981 gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta aaaaattacc
    14041 tgaccgagca ccctgaagcg accgatcccc gggattattt gcagtcggct aaatccgcaa
    14101 tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg gcataacgca
    14161 cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa agctggtgaa
    14221 catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc ggcgctggca
    14281 tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa
    14341 tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt tacgattgcc
    14401 gataaagtcg gatttgcacg cgaacgcatt attctcggcg gcgatcatct ggggccaaac
    14461 tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca
    14521 tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg cgcgggggat
    14581 cccataccgt tagcaccaga aacggttgcg gaacgagctg ctttgctttg ctgcggaaag
    14641 tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg aagttccggt
    14701 tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg aagatgccgc
    14761 caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag aggcgttaac
    14821 acgcgtgatt gccatcgtgg tgcagccggg cgtggaattt gatcacagca atattatcca
    14881 ttatcagccg caggaagcgc agccgctggc gcaatggata gaaaacaccc gaatggttta
    14941 tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag tccgcgatca
    15001 ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg cgatatttgc
    15061 actggcacaa attgagcagg aacttatcgc ccctgaaaat cgcagcggtt gcctggcggt
    15121 aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc gtacgggttt
    15181 taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt attactggcc
    15241 gcatagtcgg attaaaaata gcgtcgaaac gatgatggtg aatcttgaag gcgtggacat
    15301 cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc agtccgggga
    15361 attatcagca ataccgcatc agctgattat ggataaaatt tatgatgttt tgcgcgccta
    15421 tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc gtagcggaat
    15481 ttcttttgtc gatcgtagcg aagttttaac ccatatcggt aatgagatgc tcgccaaagg
    15541 tgtggttcat gatacctggc cacaggcatt aattgccaga ga
//
LOCUS       DAZOYL010000073        15451 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZOYL010000073 DAZOYL010000000
VERSION     DAZOYL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.073"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(273..1241)
                     /gene="astE"
                     /locus_tag="ACHUOY_001676"
     CDS             complement(273..1241)
                     /gene="astE"
                     /locus_tag="ACHUOY_001676"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR0682476.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1234..2577)
                     /gene="astB"
                     /locus_tag="ACHUOY_001677"
     CDS             complement(1234..2577)
                     /gene="astB"
                     /locus_tag="ACHUOY_001677"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR0682477.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(2574..4052)
                     /gene="astD"
                     /locus_tag="ACHUOY_001678"
                     /pseudo
     CDS             complement(2574..4052)
                     /gene="astD"
                     /locus_tag="ACHUOY_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4049..5083)
                     /gene="astA"
                     /locus_tag="ACHUOY_001679"
     CDS             complement(4049..5083)
                     /gene="astA"
                     /locus_tag="ACHUOY_001679"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR0682478.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5080..6300)
                     /gene="astC"
                     /locus_tag="ACHUOY_001680"
     CDS             complement(5080..6300)
                     /gene="astC"
                     /locus_tag="ACHUOY_001680"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR0682479.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            6746..7552
                     /gene="xthA"
                     /locus_tag="ACHUOY_001681"
     CDS             6746..7552
                     /gene="xthA"
                     /locus_tag="ACHUOY_001681"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR0682480.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            7719..8429
                     /locus_tag="ACHUOY_001682"
     CDS             7719..8429
                     /locus_tag="ACHUOY_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0682481.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8434..9111
                     /gene="ydjY"
                     /locus_tag="ACHUOY_001683"
     CDS             8434..9111
                     /gene="ydjY"
                     /locus_tag="ACHUOY_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR0682482.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9126..9833
                     /locus_tag="ACHUOY_001684"
     CDS             9126..9833
                     /locus_tag="ACHUOY_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0682483.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            9833..10381
                     /locus_tag="ACHUOY_001685"
     CDS             9833..10381
                     /locus_tag="ACHUOY_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR0682484.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10391..11557
                     /locus_tag="ACHUOY_001686"
     CDS             10391..11557
                     /locus_tag="ACHUOY_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0682485.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            11530..13066
                     /locus_tag="ACHUOY_001687"
                     /pseudo
     CDS             11530..13066
                     /locus_tag="ACHUOY_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13066..13719
                     /locus_tag="ACHUOY_001688"
     CDS             13066..13719
                     /locus_tag="ACHUOY_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0682486.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            13786..15093
                     /gene="ynjE"
                     /locus_tag="ACHUOY_001689"
     CDS             13786..15093
                     /gene="ynjE"
                     /locus_tag="ACHUOY_001689"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR0682487.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15102..>15451)
                     /locus_tag="ACHUOY_001690"
     CDS             complement(15102..>15451)
                     /locus_tag="ACHUOY_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0682488.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3616 a         4224 c         4027 g         3584 t
ORIGIN      
        1 tgacgagatt aactttactg actttagcgt caattaatca gagcaacggt aaaacaatga
       61 aagtgtaaaa aacacttttg cgccaattat ggagaaaaaa agaaaatttg atggagagtg
      121 atgagagaat attacaacac aatgattttg cagagattat gaagaactat accggatgac
      181 tggtgataaa taaagcaaat aaccaggatt aatctgtatt aatttataag aaagcaactt
      241 aatacccgca gaatgatttc tgcgggtaag tattagctta ttttttcgag cattaatccc
      301 gcgcgtaatc ccaacgctac caacggatta gggaataaca catactctac atcatgggtt
      361 acggtaaaac gttcctctcc gtcctgcgcc agcaatgttc ctttctcaaa cggcataaaa
      421 ttcagcgtgt cacttgccat atgcatttcg aaggacggcg agtggcgagt aatttgcgaa
      481 accacccgat aacggagcgg cggtgttctc acgataccga cactctcacc agatagcagc
      541 gcagcaattg cgctggcagt tactgcaaac tggcgaagat cgttttgccc aaagggcaac
      601 gctttgccaa gttccagcgt acaggccagc gcgccaaaat gtctggcgct gaaatgggta
      661 aacgtaccac caggctcctg atggaacacc agcgcctcca gccccgccgc acccagccac
      721 gtcagaaatt tctcgtccca gggaatgtcg cgttgcggta atacaccgaa ctgaggatgc
      781 aaggagccac gaattgcggt atgtagatca aggtgccaac gcacagattc tttgccctgg
      841 tcataaaaat cttccaggca ctgttccagt tcgcgcgccc gacaggtttc tccgctttca
      901 gcaaatagct gccaacgacc gccaaacatt cggttcatat cgctatggca ataacgtttc
      961 ccttgcttca gcgcaggagg atccccgagg atcaccagca accgccaacg taacgggatc
     1021 tcgccgtgag atatcgcgcc aagcaacgcg tccagcattt ccacaggtgc cgtctcatta
     1081 ccgtggatcc ccgcagaaat caccagcgcg ccttgcggtg gcgttaatgg cgtcagttcc
     1141 agcacaccat cgcccagcca gcgccagcga acgccgttga tttctcgctc ggtgataacc
     1201 ggttttttac ccgttaaggt cagagcaaga aaattatcca ttgccccctc cctcgcgctg
     1261 gaacggataa accgaaccga gattcagtaa ttggctcaat acatccagtg cttcccgccc
     1321 ttcgcgcagc aattgcgggt cggccagatc ggcagcagta aggcgatcgc ggtagtaacg
     1381 atccacccag tcattgagcg cattaaacag cgtatcgttc atcatcaccg ccggattcac
     1441 cgcccggcgt tcttcttctg tcaatactac ccgcaaccgc aggcacgccg ggccgccgcc
     1501 attcgccatg ctttcacgta aatcaaagac ttttagttcg ctaatcgggt tgtcagcggc
     1561 aaggagttca ttgagataac cccatactcc ggcgtgttcc cgacactcct gaggcagcac
     1621 caacatcatg gaaccatcat cgcggctcaa cagttggctg ttaaacagat aggtagacac
     1681 cgcatccgac acggaaacct gagttgccgg aacttctatc gccataaaac cgttcacccg
     1741 cgcccgcagg tttgccagta actgtgactg gcgagcgaac gcctgttggt ggcaaaacag
     1801 cacctggcgg ttacttacgg caatcacgtc attatgaaaa acaccctggt cgataacgtc
     1861 cgggttttgc tgggcgaaaa tcacctgttg gggattcacc tgattaagcc ttgccaccgc
     1921 ctcgctggct tcgcgagtct gtcgcgccgg ataacgggaa ggccgggtat cattgccttc
     1981 ttctcgcccg tagacaaaaa gttgcatacc cggctcaccg taatgaccgc cgagacgatt
     2041 gtggtttgcc gccccctcat caccgagcaa cgctacctgc ggcaacgccg aatggacgct
     2101 aaatttctct tcgtcgttaa aaatagcttt taacagcgat tcagtgacgg gcgcttccag
     2161 cgaacggtga aatttattgt tcaggttggc aacggtgaga tgcactttac catccagcgt
     2221 atcggcagat ggcgcgatcg ttgccgcatt ggctacccac attggcgaag cggagctgac
     2281 gctggaaagc cagtgcggtg cctggcgtgc aactttttcc agtacctgct catcgctacc
     2341 actgaatccc aactgacgca gcaccggaat aaacggacgc tcgtgcggcg ggatcacggc
     2401 ctgggggaat cccgcatcgg caagggtttt cattttcagt aagccctgtt tcgccgccag
     2461 tcgcgggtta gacacctgaa aacggtgacg ggtagaggct tcattaccaa acgacaggcc
     2521 agcgtaatga tgcgtcagcc ctaccagccc gtcgaaatta acttcccagg cgttcatcgc
     2581 accacctcat cggaaaaatc cagcccgggg ttaagcgttg cgggcaaagt taacgagtcc
     2641 gactccaggc tcgccatagg ccatgcgcag taatctgcgg cataccaggc gctggggcga
     2701 tggttaccgg atgcaccaat gccgccgaat ggcgcggtac tggcagcacc agtaagcggt
     2761 ttgttccagt taacaatccc cacccgcgcc tccagcaaca gttgatcgaa tttttcccgc
     2821 tcgggggaaa ccagaccgca agagagtccg aagcgagtgt tattcgccat tagaatcgct
     2881 tcctcgaaag aatcataacg ccagacgcgc aataacggtc cgaacacctc ttcatctggt
     2941 acgccagcaa cgcctgtcat ttcaatgatc cccggcgtca gcaacgatgt ctctgattgt
     3001 aataagcgcg gcgcaagcag ggttcgtccg cccatcgctt ccagttgctg ctaggcagta
     3061 accacctgct gtgcggcctg ttcagaaatc agcccgccaa taaacggctg cggttcgtca
     3121 tcccagttgc ccggcgttaa tcgctggctg acggcaacca gacgagcaag aaacgcatcg
     3181 ccctgtgctc cgcttttgag caataaacgg cgggcgcagg tgcagcgttg cccggctgtg
     3241 acaaacgccg actgaatggt cagatggaca gccgagtcga tatccgccac ctcatcgata
     3301 attagcggat tattaccgcc catctcaagg gcgagaattt tctccggctg accggagagc
     3361 tggcgatgca gctggtagcc ggtattggcg ctaccggtaa acagcaaacc gtcgagatcc
     3421 tccagcgcac tcagcgcctg acccgtttca cgcccgccct gcaccaggtt cagcacgccc
     3481 ggcggcaagc cagcctgctg ccataaacgc attaccgctt cgccactcca cggtgtcagt
     3541 tcgctgggtt taaagataat ggtgttacct gccagcaatg ccggaacgat atgtccgttc
     3601 ggcaaatgac cagggaaatt atacggccca aacaccgcca gcacgccgtg cgggcgatgt
     3661 cgcaggctcg ccgcaccgtc cggcatttca ctacgctgct cgccggtacg aacgtgatac
     3721 gccttaattg atatcgcgat tttattgatc atcgccgtca cttcggttgc cgcttcccag
     3781 cgcggcttac ccgtttctct ggcaataatc gcggttaatt cggctttatt gctttccagc
     3841 aatccggcaa agcgttcgac aacgacctga cgttcagcca atgagagccg cgcccagcgc
     3901 ggaaacgccg cacgggctgc ccgacaagcc tgttcgacct gagcggcatc ggcatcattg
     3961 ccttgccata acacctcgcc cgataccgga ttacgcttca cacgcgatgc gccctggccc
     4021 gttatccagt caccgttaat ccataaagtc atgctgtttt ctcctctgcg cacaggcgca
     4081 ccagacgaac gcgatcccct gcgtggcatt tgagggcatc cagttgtgcg gcggttaaaa
     4141 tcaaacgctc ggttgccgga tcggtacgcg ccagcaccac gcggaaatgg tgataatttt
     4201 cattggcgac caggcaggcc ggaaaatcgc cctgcgcagg ctgcccttct gccacttcca
     4261 ccagccgact tttacggatg gcgcgcacgc ggtcgatgtc acactcaagc gttggcccgc
     4321 cgtcgaagat gtcgatatag ttgcggtaac gaaaaccttc tttctccagc accgcgcggg
     4381 caggcgcggt ttgcggatgt acctgaccga tgacgtcctg ggcttcctgg gataaaaagt
     4441 gggtatagat cggatgtttc ggcatcagtt ctgcaataaa tgccttttgc ccggtgccgc
     4501 agagaaaatc gacgcggcta aaatccatcg aaaagaagcg tttaccgagg ctttgccaga
     4561 acggtgaata gccgtgttcg tcaatcaccc cgcgcatttc agcaaccact ttgtcattaa
     4621 acttgtcgcg aaaagccgcc ataaacataa agcgcgattt cgacagcaaa tagccgttgc
     4681 cctctttgcg ccagtccggg tcgagaaaca gcgtgcacag ctcgctgctg ccggtgtgat
     4741 cgttactaag aaacagcgtc ggcaatgcgt tatagacatt cagctctttt gaggcgtgaa
     4801 ccaacgtgcc gacccgatag ttgtaccagg gatcgttcag cccaaccgcc acctcaatgg
     4861 cgcaaatccc cgccacggtg cctgtctcgc tatcttccag cacgaacaca tagccctgct
     4921 cgcttttggg cagttcgcct tgccaggttt tgattgccct ttcgatacgc gcggaaagcg
     4981 tggcttcatt ggcgggaagc gacgtcaggc cgccgcccgt tttgctggca agctgcatca
     5041 gcgccgagac atctgagcgc tcaacgggac ggatgaccat catgatgaac ctcggctaac
     5101 aaagtgttcg caagcagctg caaagcgatc cagtccggtc gtcacctctt cttcgctgac
     5161 attgagcgca ggcgcaaaac gcaccacgtt gccacccgca atcagtacca taacgcctgc
     5221 tttcgccgcc tcctgagaga tctgtttcgc ttgtccggcg taatcggcat tcagtacaca
     5281 gccaatcaac aaacctaagc cgcgaacttc actgaacaaa ccgtagcggt gattaatggt
     5341 attaagacgc tcaacaaacc agtcgtgacg ctgtttaacg ccattaagca tctctggtgt
     5401 gttgatgagc tccagcactt tgcccgccac cgccgaggcc agcggattgc cgccataggt
     5461 ggtgccatga gtgccaacgg tcatcacgct ggcgcactct tcggttgtta acaacgcacc
     5521 gaccgggaaa ccgccgccca gcgctttggc ggtagttaac agatcaggtg tcacgccgta
     5581 gtgcatatag gcatacagtt ccccggtgcg cccgacgccg gtttgtactt catcaaaaat
     5641 caacagcgca ttgtggcggt cacacaattc acgcagacct tgtaaaaacg cgttgctggc
     5701 tggcaccaca ccgccttccc cctggatggg ttcgacaatc accgcacagg tggcgtcgtc
     5761 aatcagcgcg ctggcagagt taatatcgtt atatgcagca tgacggatat ctggtggcag
     5821 tggcgcaaaa tcctgtgaat aggctggctg cccacccgca ctgacagtaa acagcgtgcg
     5881 accatgaaac gcatttttaa acgccacgat accgctctta tggctgccgt agcggtcgtg
     5941 agcgaattta cgcgccagtt ttagcgccgc ttcgttggct tccgcaccgg agttacaaaa
     6001 gaagacgcga tcggcaaacg tggcgtcgat caattttttc gccagtcgca gtaccggctc
     6061 gttggtgtaa ccgttgccgg tatgccagaa cttactcgcc tgttcgttca gcgcttcacg
     6121 cagttccgga tgcgcatggc ccagcgcgtt caccgcaatg ccacccgcga agtcgatata
     6181 ctctttcccc tgctgatccc acaagcgcga accttcgcca cgtaccggta taaagggtgc
     6241 cggagcgtaa acaggtatca tccattcatc aaagttttca cgcgtaattg gctgagacat
     6301 agcgacctct acagtaaata attcgctatc tatatgttaa caataagtaa tgtttgcgct
     6361 gtaaatgtag attgcagggt tcgtgccagc cagtgataaa agtgcataaa cggcggaggc
     6421 taactggaaa tcaaggagtt ataaccaaac catatgcatt taaagtgcat ataaagtgaa
     6481 tacgtttgcg atgtgggtga ataaaaagaa taaaaaacgc aatgttatgc agaagtaaaa
     6541 tataattctg gaattgtgat cattgacgaa atttactgga aattactgcg ccattctgac
     6601 gcagcgcgca ccaaaagcgg gcattttttg cgccatcgtt gacatcatta acaaccatcg
     6661 atcaaatcac ttaacaacag gcggtaagca acgcgaaatt ctgctaccat ccacgcactc
     6721 tttatctgaa taaatggcag cgactatgaa atttgtctct tttaatatca acggcctgcg
     6781 cgccagacct caccagcttg aagccatcgt cgaaaagcac caaccggatg tgattggcct
     6841 gcaggagaca aaagttcatg acgatatgtt tccgctcgaa gaggtggcga agctcggcta
     6901 caacgtgttt tatcacgggc agaaaggcca ttatggcgtg gcgctgctga ccaaagagac
     6961 gccgattgcc gtgcgtcgcg gctttcccgg tgacgacgaa gaggcgcagc ggcggattat
     7021 tatggcggaa atcccctcac tgctgggtaa tgtcaccgtg atcaacggtt acttcccgca
     7081 gggtgaaagc cgcgaccatc cgataaaatt cccggcaaaa gcgcagtttt atcagaatct
     7141 gcaaaactac ctggaaaccg aactcaaacg tgataatccg gtactgatta tgggcgatat
     7201 gaatatcagc cctacagatc tggatatcgg cattggcgaa gaaaaccgta agcgctggct
     7261 gcgtaccggt aaatgctctt tcctgccgga agagcgcgaa tggatggaca ggctgatgag
     7321 ctgggggttg gtcgatacct tccgccatgc taatccgcaa acagcagatc gtttctcatg
     7381 gtttgattac cgctcaaaag gttttgacga taaccgtggt ctgcgcatcg acctgctgct
     7441 cgccagccaa ccgctggcag aatgttgcgt agaaaccggc atcgactatg aaattcgcag
     7501 catggaaaaa ccgtccgatc acgcccccgt ctgggcgacc ttccgccgct aatttagcag
     7561 ctctcctggc tcaaactggg tcaggagaat taaccttgag aaaaatcaac aaactgtcag
     7621 taatgatttg ttgcctgccg tcctttgtta taccgtcact gcgtttttag ttgtctgacc
     7681 acttctctat tatcaagttt gatataggaa actccacgat gaatgctgag cgtaaatttc
     7741 ttttcgcctg tcttattttt gcgctggtca tttacgctat ccacgctttc ggtttattcg
     7801 atctgctcac cgatttaccc cacttacaga cactcattcg ccagagcgga ttgttcggtt
     7861 atagcctcta tattctgtta ttcatcattg ccaccctctt tgtgttacca ggaagcatac
     7921 tggtgatcgc tggaggaata gtttttggcc cgctcttagg gacactactc tcattaattg
     7981 ccgccacgct ggcctcctcg tgctcattcc tgctggcgcg gtggctggga cgtgatttac
     8041 tgctgaaata cgtgggccat agccatacct tccaggccat tgaaaaaggc attgcgcgta
     8101 acggtattga ttttcttatt ctgacccgct taatcccgtt gtttccttac aatattcaaa
     8161 attacgctta cggattaacc accatcgcct tctggcctta tacccttatt tcggcactca
     8221 cgaccctgcc cggtattgtt atttataccg tgatggcaag cgatctcgcc aatgaaggca
     8281 ttacgctgcg ctttatttta caactctgtc tggcgggcct ggcgctgttt attctcgtcc
     8341 agctcgcaaa actctacgcc cgacacaaac atgtggatct gtctgcttcg cgccgcagcc
     8401 cgcttactca cccaaaaaat gaaggataga acgatgtcgc aacattattc agtgtcatgg
     8461 aaaaaaggac tggctgcact ctgcttactg gctgttgccg ggcttagcgg ctgcgatcaa
     8521 aaagagaatg cggcggcaaa agtggaatac gacggacttt cgaacagcca accgctgcgc
     8581 gtcgatgcca ataaccatac ggtaaccatg ttggtgcaaa ttaatggtcg tttcctcacc
     8641 gacgacactc gtcacggtat tgtgtttaaa gatggctcca acggacataa atcgctgttt
     8701 atggcttatg cgaccccgaa agcattttat gaagccctga aagaggcagg tggtacgccg
     8761 ggcgaaaaca tgacgatgga taataaagaa acgactcatg tcacaggcag caaactggat
     8821 atttcggtca actggcaagg ggcggcaaaa gcgtattcct tcgatgaagt gattgttgac
     8881 agtaatggca agaaactgga catgcgcttt ggcggtaatt taacggcagc agaagagaag
     8941 aaaacgggtt gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag caatgcaaca
     9001 tacacttatg gtgcggtgga aaaacgcggc gaagtgaaat tcaaaggcaa tgcctcagtt
     9061 ctcccggcgg ataacacgct ggcaacggtt acctttaaaa tcaccgaata aagccaggat
     9121 aaaggatgat gaagatgcaa tcgcgtaaaa tctggtacta ccgtataacc ctcatcatcc
     9181 tgttgttcgc tatgctgctc gcatgggcgc tgcttcccgg cgtccatgag tttatcaatc
     9241 gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat agaacgcttt attcagtctt
     9301 acggcgcact ggcggcagtt gtctcgttct tgttgatgat tttacaggcc attgctgcac
     9361 cgctacctgc gtttttgatc acctttgcca atgcgtcgct gtttggcgcg ttctggggcg
     9421 gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct gtgctttttt atcgccagag
     9481 tgatgggccg cgaagtggtg gaaaaattaa ccggcaaaac cgtgcttgac agtatggacg
     9541 gctttttcac tcgctacggc aaacacacca tcctggtctg tcggttattg ccttttgtcc
     9601 ctttcgatcc aatcagctat gctgccgatc tgacttcaat acgttttcgc tcgtttttta
     9661 tcgccaccgg gcttggtcaa ttaccgacga ctattgttta ttcctgggcg ggcagcatgt
     9721 taacaggcgg tactttctgg tttgtcaccg gactgtttat tctgtttgcc ctgaccgtgg
     9781 tgatttttat ggcgaagaaa atatggcttg agcgccagaa gaggaatgac tgatgggttt
     9841 accgccgctt agcaaaattc ctttcatttt acgtccgcag gcgtggctgc atcgtcgcca
     9901 ttacggcgag gtgctaagcc cgattcgctg gtgggggcgg atcccgttta tcttttattt
     9961 ggtgtcaatg tttgttgggt ggctggagcg caaacgctca ccgctcgatc cggtagtgcg
    10021 atcgcttgtc agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgtg tggatatcac
    10081 cagtatgaaa gtcgccgagc gtaccggcag cagcgataaa ctgctggcag tggctgactg
    10141 gcggcaaaac ccgctcttta gcgatgaaga acggctggcg ctggagtacg ccgaagccgc
    10201 aagcgtcaca ccgccaacag tcgatgatgc cctgcgtacc cgactggctg cgcattttga
    10261 cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg caaaacctgt cggctcgttt
    10321 taattctgcc atggacattc ccgctcaggg gctgtgccgt attccggaaa aacgttctta
    10381 aggagagaag atgcgccatt gtgggtggtt gctgggattg ttatcgctgt tttctttggc
    10441 aacacatgcc agtgactggc aggaaattaa aaatgaggcc aaagggcaaa ccgtctggtt
    10501 taacgcctgg ggcggcgata ccgcaattaa ccgctatctc gactgggtta gcggcgagat
    10561 gaaaacccat tacgctataa acctgaagat tgttcgtctc gcggatgccg cagacgcggt
    10621 gaagcgcatt cagaccgaag ctgctgccgg tcgtaaaacg ggcggctcgg tggatctgct
    10681 ctgggtgaac ggcgaaaact tccgcacctt aaaagaggcc aatttattac aaacgggctg
    10741 ggcggagact ctgcccaact ggcgctatgt cgacacacag ctgccggtgc gggaagattt
    10801 ttcagtgccg acacaaggtg cggaatcgcc ctggggcggc gcacaactga cgtttatcgc
    10861 ccgccgcgat gttacgccac agccaccaca aacgccgcaa gccttactgg agtttgctaa
    10921 agccaatccc ggcacggtta cttatccgcg cccaccggac tttaccggca cggcgtttct
    10981 tgaacagttg ctgattatgc tgacgccaga tcccgccgca ttaaaagaag cgccggacga
    11041 tgcgactttc gcccgtgtca ctgcgccctt gtggcaatat cttgatgcac tgcatccgta
    11101 tttgtggcgc gaaggaaagg atttcccgcc ttcgcccgcg cggatggatg ctctgctgaa
    11161 agccggcaca ttgcgcctgt cgctgacctt taaccccgcg catgcgcagc aaaaaatcgc
    11221 cagcggcgat ttgccggcaa gcagttacag ttttggcttt cgcgagggga tgattggcaa
    11281 cgtgcatttc gtcaccattc cagccaacgc gaatgccagt gctgcggcga aggtagttgc
    11341 caatttcctg ctctcacccg atgcgcaact gcgtaaagca gatcccgttg tctggggcga
    11401 tccttctgtt ctcgatccgc aaaaactgcc tgacgggcag cgcgaaatat tgcaatcaag
    11461 aatgccgcag gatctgccgc cggtactggc tgaaccgcac gcaggatggg tgaatgcact
    11521 ggaacaagaa tggttacgcc gttacggtac gcattaatct ttttgctgtg ggcgatggtg
    11581 gcggtgattt atgcaccgct gatcccggca gctctcacgc tgatatcgcc tgcgttgtcg
    11641 ttgacacact ggcaggcgtt atttgccgat ccacagttac cgcatgcatt actggcaacg
    11701 ctggtatcga caaccatcgc ggcggtcggg gcattgttga ttgccctgct ggtgattgtg
    11761 gcgctatagc ctgggccgaa atggcagcgt atgtgcgccc gtctgccgtg gctgctcgcc
    11821 attccccatg tggcttttgc caccagcgcc ctgctgctct ttgctgacgg agggctgcta
    11881 tatgactatt tcccgtattt cactccgcca atggaccgat ttggcatcgg tctgggcctc
    11941 acccttgcag tgaaagaaag cgcatttctg ctgtggatct tagcggcagt attgagcgaa
    12001 aaacggctgt tgcagcaggt cattgtgctg gattcgctgg gctacagccg ctggcaatgc
    12061 ctgaactggc tgctgttgcc ctccgtcgcg ccttcgctgg caatggcgat gctggcgatt
    12121 gttgcctggt cgctttcggt cgtggatgtg gcgattattc ttgggccagg taatccaccg
    12181 acgctggcag taattagctg gcagtggtta actcagggcg acgcggatca acaaacgaaa
    12241 ggcgcactcg ccagtctgct gctgatgctg ttactcgccg cctacgtttt gctgagctat
    12301 ctgctatggc gcagctggcg gcgcactatt ccccgcgtag atggcattcg caatgcctgc
    12361 cacaccttta ttaccaggca ctacgctggc gagtttttta cccttaaccg gtgtgctgtg
    12421 tgtggttctg ctggcgatcc tcgcggatca gtcgacgatc aatagtgaag cactcatcaa
    12481 cagtctgaca atggggctgg tagcaacatt catcgctttg ctcctgttac tgctgtggct
    12541 ggaatggggg ccacagcgtc gccagttgtg gctatggttg cccattctat tgcccgatct
    12601 gccactggtg gcgggccagt acacgctggc gctatggctg aacctggatg gaagctggac
    12661 ggcggtggtc tgggggcatc tgctgtgggt aatgccgtgg atgctgttta tcctgcaacc
    12721 cgcctggcag cgcattgatt cacggttaat tttgattgcg caaacactgg gctggtcgcg
    12781 ggccaaaatc ttcttttacg tgaaatgccc actcatgttg cgccctgtgc tgattgcctt
    12841 cgcggtggga ttttcagtca gtattgcgca gtatatgccg acgctgtggc tgggcgcggg
    12901 gcgttttccg acgctcacca ctgaggcggt ggcattaagc agcggcggca gcaacggtat
    12961 tctcgccgcc caggctttat ggcaactgtt gttaccgctt attatttttg ccctgaccgc
    13021 gttagtcgca aaatgggtag gttatgtcag acaaggactc cgctaatgct ctgcgtgaaa
    13081 aatgtttcgc tacgtttacc agaaagccgc ttgctgacaa acgttaactt tacggttgat
    13141 aaaggtgaca tcgtcacgtt aatggggccg tctggctgtg gaaaatccac tctgttttca
    13201 tggatgattg gtgcactggc tggacagttt tcttgtacag gtgagctatg gctcaatgag
    13261 caacggattg acatgctacc caccgcacag cgtcagattg gcattctttt ccaggatgca
    13321 ctgttatttg accagttcag cgtcgggcaa aatttactgc tggcgctact gtcgacgctc
    13381 aaggggactg ccaggcgcaa tgccgtgaaa gacgcgcttg atcgagcggg actggctgaa
    13441 acttatcacc aggaccctgc gacattatca ggcggtcagc gagcgcgggt ggccctgctg
    13501 cgcgcccttc tcgctcagcc aaaagcgtta ctcctggatg agccattcag ccgtcttgat
    13561 gtggctctgc gcgataattt tcgccagtgg gtgttcagcg aagttcgcga actggcgatc
    13621 cccgtcgttc aggtaacgca cgatctccag gatgttcctg ctgatagttc tgttctggat
    13681 atggcgcagt ggtcagaaaa ttacaacaaa ctgcgataac gcaaagtttt tctcaatgcg
    13741 tcagttcaga atggcgcact caaaactaca atgtcgggat tttcgatgaa acgtgtttct
    13801 caaatgaccg cgctggcaat ggctttaggg ctggcttgcg cttcttcgtg ggccgctgaa
    13861 ctggcgaagc ctcttacact tgaccagctt caacaacaaa atggcaaagc gatagatact
    13921 cgccccagcg cgttttataa cggctggcca caaaccttaa atggcccttc tggtcatgaa
    13981 cctgccgcct taaacctctc tgccagctgg ctcgacaaaa tgagcaccga acagctcaac
    14041 gcgtggatca agcaacataa cctgaaagcc gatgccccgg tggcgctgta cggtaatgac
    14101 aaagatgtcg acgccgtcaa aacgcgactg caaaaagcag gttttacgca tatctccatt
    14161 ctgagtgacg cgctaagcga accttcccgt ctacaaaaac tgccgcactt tgagcagctg
    14221 gtttatccgc aatggctgca cgacctgcaa caaggtaaag aggttacggc gaaacctgcc
    14281 ggtgactgga aagtcattga agcggcctgg ggcgctccta agctttacct tatcagccat
    14341 attcccggtg ctgactacat cgataccaac gaagtggaaa gcgaaccgct gtggaacaaa
    14401 gtttctgatg aacaactaaa agcgatgctg gcaaaacacg gcattcgcca tgacaccacg
    14461 gtcattctgt acgggcgtga cgtatacgct ggagcgcgtg tggcgcaaat tatgctctac
    14521 gcaggcgtga aagatgtgcg tctgctggat ggcggctggc aaacctggtc cgacgcggga
    14581 ctgcctgttg agcgaggaac gccaccgaaa gtgaaagcgg aaccggattt cggcgtgaag
    14641 ctcccggcac aaccgcagtt gatgctcgat atggaacagg cccgtggact gctgcatcgc
    14701 caggatgcat cgctggtgag cattcgttcg tggccagaat ttatcggtac gaccagcggt
    14761 tacagctata ttaaaccaaa aggtgaaata gccggagcac gttggggaca cgctggtagc
    14821 gactcgacgc atatggaaga ttttcataac ccggatggca ccatgcgcag cgccgatgat
    14881 attaccgcta tgtggaaagc gtggaatatc aaaccagagc agcaagtttc attctactgc
    14941 ggcaccggct ggcgcgcgtc cgaaaccttt atgtacgcac gcgccatggg ttggaagaat
    15001 gtttccgtgt atgacggcgg ctggtacgaa tggagcagcg atccaaaaaa tccggtagca
    15061 accggtgaac gcggcccgga cagtagcaaa taacattgca attactgacg ctggagcgac
    15121 ttcagcgtca gatacccact ccacacccgc gtaaaggtcg tcatccagca caacgcaccg
    15181 aagatccacg caaaccacgc aaaccatgca gggaataaac atcccagcac aaacagtaag
    15241 atcgtttctg tgccttcggt taatcccccc agatagtaaa acgacttatg tgcataaccg
    15301 gggttatcaa tctgatgttt tgctgccagt gcagcaaagg cgagaaaact gctgcccgtg
    15361 ccgataaacg caaacaacaa ccagccgcct gccagcgcat tttgctccgg tgcagcaaga
    15421 ataaagccaa acggcaccag cgcgtaaaag a
//
LOCUS       DAZOYL010000074        15406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZOYL010000074 DAZOYL010000000
VERSION     DAZOYL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.074"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..453
                     /locus_tag="ACHUOY_001691"
     CDS             <1..453
                     /locus_tag="ACHUOY_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0682489.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            669..758
                     /locus_tag="ACHUOY_001692"
                     /pseudo
     CDS             669..758
                     /locus_tag="ACHUOY_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHUOY_001693"
     CDS             complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHUOY_001693"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR0682490.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHUOY_001694"
     CDS             complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHUOY_001694"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR0682491.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHUOY_001695"
     CDS             complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHUOY_001695"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0682492.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHUOY_001696"
     CDS             complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHUOY_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR0682493.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3726..4514
                     /gene="modE"
                     /locus_tag="ACHUOY_001697"
     CDS             3726..4514
                     /gene="modE"
                     /locus_tag="ACHUOY_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR0682494.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4582..6054
                     /gene="modF"
                     /locus_tag="ACHUOY_001698"
     CDS             4582..6054
                     /gene="modF"
                     /locus_tag="ACHUOY_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR0682495.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6315..7331
                     /gene="galE"
                     /locus_tag="ACHUOY_001699"
     CDS             6315..7331
                     /gene="galE"
                     /locus_tag="ACHUOY_001699"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR0682496.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7341..8387
                     /gene="galT"
                     /locus_tag="ACHUOY_001700"
     CDS             7341..8387
                     /gene="galT"
                     /locus_tag="ACHUOY_001700"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR0682497.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8391..9539
                     /gene="galK"
                     /locus_tag="ACHUOY_001701"
     CDS             8391..9539
                     /gene="galK"
                     /locus_tag="ACHUOY_001701"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR0682498.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9533..10573
                     /gene="galM"
                     /locus_tag="ACHUOY_001702"
     CDS             9533..10573
                     /gene="galM"
                     /locus_tag="ACHUOY_001702"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR0682499.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHUOY_001703"
     CDS             10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHUOY_001703"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR0682500.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHUOY_001704"
     CDS             complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHUOY_001704"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR0682501.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13055..13435
                     /locus_tag="ACHUOY_001705"
     CDS             13055..13435
                     /locus_tag="ACHUOY_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR0682502.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13549..14490
                     /gene="zitB"
                     /locus_tag="ACHUOY_001706"
     CDS             13549..14490
                     /gene="zitB"
                     /locus_tag="ACHUOY_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR0682503.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHUOY_001707"
     CDS             complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHUOY_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR0682504.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15244..>15406)
                     /locus_tag="ACHUOY_001708"
     CDS             complement(15244..>15406)
                     /locus_tag="ACHUOY_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0682505.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3917 a         4042 c         3851 g         3596 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt taaaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg
      721 acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt
      781 ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg
      841 ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg
      901 tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc
      961 ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc
     1021 ggttcgtcca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg
     1081 taatgcggat gatgctcaag taccgtcact ttcagaatgc tactttgttg ctctttcggc
     1141 agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc
     1201 tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg
     1261 ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt
     1321 tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt
     1381 gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct
     1441 ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg
     1501 accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc
     1561 cgcgcatcct gaaaaacata gccaacgcga cgcttttccg gcgtcaggca gatacctttt
     1621 tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt
     1681 ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg
     1741 atgccattgg cgggcagtgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa
     1801 aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt
     1861 ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc
     1921 cactttcccc gccgggggtc tggatcaggg tatacatggc agagggaatg gttcgcgttt
     1981 cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa
     2041 aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga
     2101 aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttcgagtttg acatcaaccc
     2161 cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga
     2221 gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac
     2281 cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta
     2341 acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca
     2401 ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa
     2461 ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt
     2521 aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc
     2581 tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg
     2641 tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctgacaactg cgtcagaacc
     2701 gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc
     2761 ttccgccgga gccagtttcg gggagagcgt atcccatgcg cccagttttt gcagtgcttc
     2821 ttttgcataa atgccagcag ggacatgttc cggatcgcca accgcgaggc gaccgccatt
     2881 cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa tctttctgct cgctggcttt
     2941 cggtgctaca acgaccaggc tattgccgag cagtgtctgg cgcgaagccg tatcgatcgc
     3001 ttttttatca accgcataat ccatccattt ctgatcggca gagataaaca gatccgcagg
     3061 cgctcccgct tcaatctgac gggccagagt agatgacgaa gcgaaagaag aaaccacatc
     3121 cacgcctttt tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc
     3181 ggcggcgaac accgtgattt tcccttcatc tgccagtgta ttgccagcaa cagcgaaaga
     3241 gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta
     3301 acgttatata ggtgacaatg taacggctag ctcagaattt tcccagcagt tattgctaac
     3361 ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc aggaagttga gaaaaagaaa
     3421 tgcccgacta agcgggcatt cagggaatca atgattttgt ccgggctggt cttttttacc
     3481 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     3541 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     3601 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     3661 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     3721 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     3781 cgcgccggat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     3841 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     3901 gcgagcatat tctggttgag cgcgcaacag gcggtaaagg tggcggcggc gcagtactga
     3961 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4021 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4081 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4141 atcatgatga cattcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4201 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4261 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4321 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4381 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4441 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4501 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     4561 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     4621 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     4681 tttgtcggtt cgaatggaag cgggaaatcg gccctggccc gcgcgctggc gggggaactt
     4741 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     4801 caattgcaaa aactcgtcag cgacgaatgg cagcggaata acaccgatat gctcggccct
     4861 ggcgaagatg acaccggacg cactacggct gaaatcattc aggatgaagt aaaggatgca
     4921 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     4981 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5041 cctgacttgt tgattcttga tgagtcgttc gatggcctgg atgttgcctc acgtcagcag
     5101 ctggctgagc gactcgcctc gttacatcag tccggtatta ctctggtact ggtgctcaat
     5161 cgcttcgatg agatcccgga gtttgtccag tttgctggcg tgctggcgga ttgcacgtta
     5221 gcggaaactg gcgctaaaga ggaactgctc caacaagcac tcgtcgcgca actggcgcat
     5281 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5341 ttacccgcca acgaaccgcg cattgtgctg aacaatggcg tggtttctta taacgatcgc
     5401 cccattctta ataaccttag ctggcaggtg aatccaggtg aacactggca aattgtcggg
     5461 ccaaatggtg caggaaaatc gacgttatta agcctggtta ctggcgatca tccgcaaggt
     5521 tacagcaacg atttgacgct tttcggacga cgtcgcggca gcggcgaaac catctgggat
     5581 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     5641 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggccgtt
     5701 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacgt
     5761 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     5821 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     5881 ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     5941 caattgttat ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6001 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6061 agtgctgacc ttgccggagg cggcctaagc gccctctccg gccaacggtt cgacgcatgc
     6121 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6181 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6241 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accataagcc
     6301 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6361 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6421 gtaagcgcag cgtactgcct gttatcgagc gcttaggcgg caaacatcca acgtttgttg
     6481 aaggcgatat tcgtaacgaa gcgttgatga ccgagatact gcacgatcac gctatcgaca
     6541 ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc ggtacaaaaa ccgctggaat
     6601 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     6661 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc aaaattccat
     6721 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     6781 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     6841 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     6901 ttccgaataa cctgatgcca tacatcgccc aggttgctgt aggccgtcgc gactcgctgg
     6961 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7021 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7081 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7141 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7201 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7261 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7321 atcccgatta aggaacgacc atgacgcaat ttaatcccgt tgatcatcca catcgccgct
     7381 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7441 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7501 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     7561 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     7621 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     7681 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     7741 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     7801 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     7861 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     7921 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     7981 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8041 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8101 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8161 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8221 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8281 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8341 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8401 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8461 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     8521 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     8581 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     8641 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     8701 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     8761 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     8821 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     8881 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     8941 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9001 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9061 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9121 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9181 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9241 aaggcgatct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
     9301 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gcagtgattg
     9361 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9421 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9481 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
     9541 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
     9601 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttcag ctcgtattcc
     9661 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagcccgg aatgctatca
     9721 ggatcaggcc gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
     9781 ccgttatacc tttgacggtg aaacggtgac gctttcgcca agtcagggcg ttaaccagct
     9841 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
     9901 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
     9961 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10021 agttgataaa tcttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10081 gcagtctgac gtgcgcaatc acaagttgca gattctggcg ggcgaatatc tgccggttga
    10141 tgaaggcggc attccgcacg acggcctgaa atctgtcgcc ggaacctctt ttgatttccg
    10201 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10261 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10321 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctcccg ctcttcaatt
    10381 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10441 gcaaggactg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10501 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    10561 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    10621 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    10681 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    10741 tattatcata aaaagataat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    10801 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    10861 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    10921 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    10981 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11041 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aaccgccgaa aagtatggcg
    11101 acgagcaggt gaaacaatgg cgtcgtggtt ttgccgtgac tccgccggaa ctgactaaag
    11161 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagagctgc
    11221 cgctgactga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    11281 ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    11341 gtgcgctggt gaaatatctt gataacatga gtgaagaaga gattcttgag ctgaatatcc
    11401 cgaccggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11461 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgctaac cagggtaaag
    11521 cgaagtaaac gtcattcgtt taaaaatgag aaagccgact gcaagtgagt cggctttttt
    11581 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    11641 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    11701 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    11761 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    11821 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    11881 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    11941 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12001 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcac
    12061 cgttaccgct ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12121 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12181 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12241 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    12301 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    12361 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    12421 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    12481 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    12541 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    12601 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttacgg
    12661 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    12721 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    12781 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    12841 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagata tctcctacaa
    12901 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    12961 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13021 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13081 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13141 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13201 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13261 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaga
    13321 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaat
    13381 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    13441 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    13501 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    13561 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    13621 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    13681 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    13741 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    13801 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    13861 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    13921 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    13981 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14041 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    14101 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14161 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14221 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    14281 tggatggtag gcgagaagcc ggtgataacg ctgcatgtgc aggtgatccc gccacacgat
    14341 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    14401 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    14461 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    14521 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    14581 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    14641 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    14701 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    14761 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    14821 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    14881 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    14941 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15001 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15061 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15121 cctaccgctt cgatccatga gagatcataa ccgcctgccc ctattggtat atgtaccagg
    15181 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15241 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    15301 ctctcgcagc ctttcatcaa gaacctcgtg attgcttcct tcctgttcta atgcctctgc
    15361 gatggactga aggccattca tggccatcca cggacaatgc gcgcag
//
LOCUS       DAZOYL010000075        15185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZOYL010000075 DAZOYL010000000
VERSION     DAZOYL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.075"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHUOY_001709"
     CDS             <1..1015
                     /locus_tag="ACHUOY_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0682506.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUOY_001710"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUOY_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR0682507.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHUOY_001711"
     CDS             1797..1904
                     /locus_tag="ACHUOY_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR0682508.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUOY_001712"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUOY_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR0682509.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUOY_001713"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUOY_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR0682510.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHUOY_001714"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHUOY_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR0682511.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHUOY_001715"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHUOY_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR0682512.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHUOY_001716"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHUOY_001716"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR0682513.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="ACHUOY_001717"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="ACHUOY_001717"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0682514.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHUOY_001718"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHUOY_001718"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR0682515.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMLVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="ACHUOY_001719"
     CDS             complement(11226..11813)
                     /locus_tag="ACHUOY_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR0682516.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="ACHUOY_001720"
     CDS             11983..12561
                     /locus_tag="ACHUOY_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0682517.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="ACHUOY_001721"
     CDS             complement(12691..13452)
                     /locus_tag="ACHUOY_001721"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0682518.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHUOY_001722"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHUOY_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR0682519.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
BASE COUNT         3706 a         4149 c         3805 g         3525 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgtaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aagcatcact ttaccagttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgctaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accaacaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcggt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggct
//
LOCUS       DAZOYL010000076        15084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZOYL010000076 DAZOYL010000000
VERSION     DAZOYL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.076"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHUOY_001723"
     CDS             <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHUOY_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR0682520.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="ACHUOY_001724"
     CDS             complement(984..1337)
                     /locus_tag="ACHUOY_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR0682521.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHUOY_001725"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHUOY_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR0682522.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="ACHUOY_001726"
     CDS             complement(3165..3458)
                     /locus_tag="ACHUOY_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR0682523.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHUOY_001727"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHUOY_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR0682524.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /locus_tag="ACHUOY_001728"
     CDS             complement(5012..5482)
                     /locus_tag="ACHUOY_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR0682525.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="ACHUOY_001729"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="ACHUOY_001729"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR0682526.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHUOY_001730"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHUOY_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR0682527.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="ACHUOY_001731"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="ACHUOY_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR0682528.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHUOY_001732"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHUOY_001732"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR0682529.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="ACHUOY_001733"
     CDS             10838..11413
                     /locus_tag="ACHUOY_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0682530.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="ACHUOY_001734"
     tRNA            11520..11595
                     /locus_tag="ACHUOY_001734"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            11838..11952
                     /locus_tag="ACHUOY_001735"
                     /pseudo
     CDS             11838..11952
                     /locus_tag="ACHUOY_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            12212..12511
                     /locus_tag="ACHUOY_001736"
     CDS             12212..12511
                     /locus_tag="ACHUOY_001736"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR0682531.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHUOY_001737"
     CDS             complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHUOY_001737"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR0682532.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            12694..13749
                     /gene="cadC"
                     /locus_tag="ACHUOY_001738"
     CDS             12694..13749
                     /gene="cadC"
                     /locus_tag="ACHUOY_001738"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR0682533.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="ACHUOY_001739"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="ACHUOY_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0682534.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAZOYL010000077        15060 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZOYL010000077 DAZOYL010000000
VERSION     DAZOYL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15060
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.077"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            111..854
                     /locus_tag="ACHUOY_001740"
     CDS             111..854
                     /locus_tag="ACHUOY_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0682535.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(911..2344)
                     /gene="bglA"
                     /locus_tag="ACHUOY_001741"
     CDS             complement(911..2344)
                     /gene="bglA"
                     /locus_tag="ACHUOY_001741"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR0682536.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            2389..2700
                     /gene="yqfB"
                     /locus_tag="ACHUOY_001742"
     CDS             2389..2700
                     /gene="yqfB"
                     /locus_tag="ACHUOY_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR0682537.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            2864..3523
                     /locus_tag="ACHUOY_001743"
     CDS             2864..3523
                     /locus_tag="ACHUOY_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR0682538.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(3719..4699)
                     /gene="ygfZ"
                     /locus_tag="ACHUOY_001744"
     CDS             complement(3719..4699)
                     /gene="ygfZ"
                     /locus_tag="ACHUOY_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR0682539.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            4942..5208
                     /gene="sdhE"
                     /locus_tag="ACHUOY_001745"
     CDS             4942..5208
                     /gene="sdhE"
                     /locus_tag="ACHUOY_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR0682540.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            5189..5596
                     /locus_tag="ACHUOY_001746"
     CDS             5189..5596
                     /locus_tag="ACHUOY_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR0682541.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(5636..6157)
                     /gene="fldB"
                     /locus_tag="ACHUOY_001747"
     CDS             complement(5636..6157)
                     /gene="fldB"
                     /locus_tag="ACHUOY_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR0682542.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            6269..7165
                     /gene="xerD"
                     /locus_tag="ACHUOY_001748"
     CDS             6269..7165
                     /gene="xerD"
                     /locus_tag="ACHUOY_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR0682543.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            7190..7900
                     /gene="dsbC"
                     /locus_tag="ACHUOY_001749"
     CDS             7190..7900
                     /gene="dsbC"
                     /locus_tag="ACHUOY_001749"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR0682544.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            7906..9639
                     /gene="recJ"
                     /locus_tag="ACHUOY_001750"
     CDS             7906..9639
                     /gene="recJ"
                     /locus_tag="ACHUOY_001750"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR0682545.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            9730..10828
                     /gene="prfB"
                     /locus_tag="ACHUOY_001751"
     CDS             join(9730..9804,9806..10828)
                     /gene="prfB"
                     /locus_tag="ACHUOY_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR0682546.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            10838..12355
                     /gene="lysS"
                     /locus_tag="ACHUOY_001752"
     CDS             10838..12355
                     /gene="lysS"
                     /locus_tag="ACHUOY_001752"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0682547.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(12398..12946)
                     /gene="idi"
                     /locus_tag="ACHUOY_001753"
     CDS             complement(12398..12946)
                     /gene="idi"
                     /locus_tag="ACHUOY_001753"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR0682548.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            13001..13072
                     /locus_tag="ACHUOY_001754"
     CDS             13001..13072
                     /locus_tag="ACHUOY_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0682549.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(13069..13194)
                     /gene="yqfG"
                     /locus_tag="ACHUOY_001755"
     CDS             complement(13069..13194)
                     /gene="yqfG"
                     /locus_tag="ACHUOY_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR0682550.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(13196..14644)
                     /gene="uacT"
                     /locus_tag="ACHUOY_001756"
     CDS             complement(13196..14644)
                     /gene="uacT"
                     /locus_tag="ACHUOY_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR0682551.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         3761 a         3741 c         3961 g         3597 t
ORIGIN      
        1 gccctctccc tgaaagggcg aggggaaaag cgtgccaaca ttgaagattg agccagtttg
       61 ttagcaatct caaagatacg tcaacgaatt aatttttctc ggaaaaacaa atggctatag
      121 cacttgtgac cgatggcagt cgcggcatcg ggcgggcaac tgcattactg ttggcgaaag
      181 aagagtatac ggtggcggtt aattatcaac aaaacctcca tgcggcgcag gaagtggtga
      241 acttaatcac gcaagccggt ggcaaggcat tcgtgctcca ggcggatatc agcgacgaaa
      301 atcaggtcat cgcaatgttt acagcaatcg atcagcacga tgaaccgcta gcagcgctgg
      361 tcaataacgc cgggatcttg tttacccagt gcaccgttga aaaccttacc gcagagcgaa
      421 tcaaccgagt actttccacc aacatgacgg gatattttct ctgctgccgc gaggcggtaa
      481 aacgcatggc gcttaaaaat ggtggcagtg gcggcgctat cgtcaatgtc ttttcggtgg
      541 cctcacggtt gggttcgcca ggggaatatg ttgattacgc ggcatcgaaa ggggcgattg
      601 atacgttaac caccggacta tcgctggaag tcgccgcgca gggggtccgc gttaactgcg
      661 tgcggccagg gtttattttt accgaaatgc acgccagcgg cggcgagcct ggacgcgtcg
      721 atcgcgttaa gtcgaacatc cccatgcagc gtggtggaca ggcagaagag gtcgcgcagg
      781 ccattgtctg gctactaagt gataaagcct cttacgtcac gggaagtttt atcgatttgg
      841 cgggcgggaa ataaaacagg gaagttgtct gaccggatgc aacaagtatt gcatccggta
      901 cttcatcgac ttaaagcttc tcgccgttgc tggtaatcac ctctttgtac cagttaaagc
      961 tcttcttacg tgaacgcgac atatcgccag taccgtcgtc atgtttattc acatagataa
     1021 agccgtagcg tttgctgtac tgcccggtgg tgaacgatac gcagtcgatg cagccccacg
     1081 gtgtgtagcc catcagatcc acgccatcgt aagtcaccgc ttttttcatc tcttcgatat
     1141 gggcgcgcag gtagtcaatg cggtagtcgt cgttgatgct gccatcttct tccactttgt
     1201 cgtaagcgcc aaaaccgttt tcgacaataa acagcggcct ctgataacgc tcatacagtt
     1261 cgcaaagggc atagcgcaga cctactggat caatctgcca gccccagtca gacgctttaa
     1321 catacgggtt cggtacgctg ccttcgaaac cagagatcgc atcgccggtg ccgccttcgg
     1381 ccttcactgc gttggtcatg taatagctga aaccaagata atcgcaggtg ccttcgcgca
     1441 gtacatcgag atcgccgtct tccattttga tgttaaatcc gcgacgctcc cactcgttca
     1501 acacatagga cgggtaatag ccgcgtagct gcacatcggt aaagacgtag cgttcgcgca
     1561 tcgactcctg agcgaacatc acatcgtccg ggttacagca gtagggatag agcggcacca
     1621 tcgccagcat acagccgact ttcatctccg ggttaatgcg acgcgcagct ttcaccgcca
     1681 gggcgctggc gacaaactgg tgatgcagca cctgatacat cgtctcttcc gggttctcat
     1741 gctcggtata aaccacgccg gagcagcagt aaccgaacag cggtgcacgc cagttacgct
     1801 ggttgttaat ttcgttgaag gtcatccagt atttgacttt atgcttatag cgttcaaaca
     1861 ccacttccgc gaaacggaca aagaaatcaa ccactttacg gttggtccag ctaccgtatt
     1921 gctgcaccag atgcagcggc atttcaaagt gggagagggt gatcaccggt tcgatgttgt
     1981 atttcagcag ttcatcgaac atatcatcgt agaacttcag cccttcttcg tttggctgag
     2041 cttcatcgcc ttttggaaaa atgcgcgtcc aggctatgga agtccgaaaa catttgaagc
     2101 ccatttcggc aaatagcttg atgtcttcct tgtagtgacc ataaaaatca acggcttcat
     2161 ggtttggata gtatttgcct ggcaagactt ctttggtgat ttcgcgcggc acgccgtgtg
     2221 cgccaccggt cagaacgtca caaatgctcg gccctttgcc gcctttgttc cagccgcctt
     2281 cgacctgatg agcggcaact gcgccgcccc ataagaaatc tttctgtaag gtgagttttt
     2341 tcactatcat aggctccaga tgagtcaatt cttacaggaa atagtaccat gcagccaaac
     2401 gacatcactt tttttcaacg tttccaggat gacattctgg ctgggcgtaa aaccatcacc
     2461 atccgcgacg agtctgaatc gcacttcaaa acgggtgatg tgcttcgtgt cgggcgtttt
     2521 gaagatgacg gttatttttg cacgattgaa gtcaccgcaa cctcaaccgt aacgctggat
     2581 acgctgacgg aaaaacatgc agagcaggaa aatatgaccc tgactgaact gataaaggtc
     2641 attgccgaca tctatcccgg tcagacacag ttttatgtga ttgaatttaa atgtctttaa
     2701 attgaactca cgcctgttag ttgaaatttc gcatttaatt tcatgatttt taggagatta
     2761 gtctcaaatc agaatttatt ttagctaaca ggtgttcact ggaactattc tcagttacgc
     2821 tggagaggta tacatgtgcg tgtacgtttc tccggagtaa gttatggttc agaagcccct
     2881 cattaagcag ggatattcgc tggcagagga aattgccaac agcgtcagtc acggcattgg
     2941 gttggtgttt ggtatcgttg ggctggtgtt gctactggtt caggcggtgg atctcaatgc
     3001 cagcgccaca gcgataacca gttacagcct ctatggcggc agtatgatcc tgctgttcct
     3061 cgcttcgacg ctctatcacg ccattcctca tcaacgggca aaaatgtggc tgaagaaatt
     3121 tgaccattgc gctatttacc tgttgattgc cggaacctac acgccgtttt tgctggtggg
     3181 gctggattct ccgttagcgc gcgggttgat gattgttatc tggagcctgg cattgctggg
     3241 tattctgttt aaactgacca tcgcgcaccg attcaaaatt ttatctctgg tgacctatct
     3301 ggcgatgggc tggctgtcgc tggtggtaat ttatgaaatg gcagttaagc tcgcggcggg
     3361 cagcgttacc ttattggcgg taggcggcgt ggtttattcg ctcggagtga ttttctatgt
     3421 ctgcaaacgc attccctaca atcatgccat ctggcacggc ttcgtgctcg gcggcagtgt
     3481 gtgccacttt ctggcgatct atttgtatat tgggcaggcg taatgggtat gcctttgtag
     3541 gcctgaaaag acgcgtcagc gtcgcatcag gcatcggagc acaactgccg gattcggcgt
     3601 gaacgcctta tccgatctac gggcctcgtc cctgttgtga taaaggcatc aaatcccaga
     3661 catgataaga cgcgtcagcg tcgcatcagg cgataaatgc ctgatgcgat aaagagattt
     3721 actcttcgag cgaatacggc agcggctcaa tatgcaatgt attcgcatcg tcgcgtacgc
     3781 ggaagatgct atccggttcc atatcgttat tcatgacgac ctgtaccacg acctgaccat
     3841 cttccagttt taccgcagcc agcaccgtac cggtacgacg ccagttctcg cccattttca
     3901 gctctaagtc ttcaccagct tccggcagtc ggctggcgct acctgccagc aaccagagcg
     3961 cacgtttatt ggcaccacgg aattttgctc gcgccaccat ctcttgtccg gtataacagc
     4021 ctttcttaaa gctgataccg cccagcgcct ggagattggt cgcctgtggg ataaactgcc
     4081 cgctgttggc ggcatcaatc accgggaaac ccgcttcaat gtttaatgcc agccactgtt
     4141 ggctattgtt cagttccgct tcaccgcgca gtttatcggt cagcatatta gcagtagctt
     4201 catcggttac gatcaggaaa cgttctgccg ggtattcaaa ccatagcaaa gtggtcgcgc
     4261 cttctttaac tacctgtttt tctttcgaag gcagttcact aaagatattt gccagcgcgg
     4321 cgcgcgcctg aaaaccggca acaccaagca gcacatgctc gtcgtctggc gcgatggtca
     4381 ctttagagaa caccgcatat tttttcagtt cagtcagctg cggttcacgc acgctgcgcc
     4441 gttcaatcca cgcaaagcca tcgccgtcgc ggaacagacg taaattgctc cacattttgc
     4501 ctttggcgtc gcaatgggcg gcgagcaggt gctgatcttc tgccatctgg ctgacatctg
     4561 ctgtcacctg accctgcata tatttttcgc tgtccgcgcc agtaatggtg gcaagcgccc
     4621 agtcatcaag cgtcatcagc gtcagcggta aacgggcaga agccgtaggc tgacggggag
     4681 gaaaaggtgt aaaagccata atagagtcct gagttgctta acggcgtatt aatgactaat
     4741 ggtaaaagag ctatgaggca atgcaagtgc tttcactacg cgatctgtgc cttctgcgag
     4801 gcgacttcca aaaaaatgaa gccattgaat tgttgtgaat taatttcgtg atcgcgatgg
     4861 tgtttactga tattcccgta aaagaaggcg aaaaacacgt tacaatagcg gggtactgga
     4921 ttttcgtgag acaggaagaa gatggacatt aacaacaaag cccgcattca ttgggcatgc
     4981 cgccgtggta tgcgcgaact cgatatttca atcatgccgt ttttcgaaca tgagtacgac
     5041 agcttaagcg atgacgaaaa acgcatcttt attcgtctgc tggaatgtga cgatccggac
     5101 ctgtttaact ggctgatgaa tcacggtaaa ccggccgatg cagaactgga aatgatggtc
     5161 cgactcatcc agacacggaa ccgggaacgt ggtcctgtgg caatctgatt tgcgcgtctc
     5221 ctggcgcgca cagtggcttt ccttgctgat tcatgggctg gttgccgctg ttattttact
     5281 catgccctgg ccactcagtt acaccccgtt atggatggtg ttactttcgc tggtggtgtt
     5341 tgattgcgtt cgcagccagc ggcgtattaa tgctcgccag ggggaaattc gcttgttgat
     5401 ggacgggcgt ttgcgttggc aagggcagga gtggagcatc gtcaaagcac cgtggatgat
     5461 taagagcggc atgatgctgc gtttacgttc tgatggcggt aaacggcaac atttatggct
     5521 ggcagccgac agcatggacg aagccgaatg gcgggattta cggcggattt tgttgcaaca
     5581 agagacgcaa agataaagat gacggcaaag catatgcgct aagccgtcag tgagatcagg
     5641 cgtaatgctc tgccatttcg ttgaggattt gctcgcacca gctctgaata cgctcgtcgc
     5701 taaggtcata ctggttagtt tcatccagcg ccagacccac gaacagttgc ccgtcagcaa
     5761 tcaccggttt cgggctggta aattcatatc cttccgttgg ccagtagccg acgaacttca
     5821 cgcctttggt cgagagtttg tcatgcagca taccgagcgc atcgaggaac cactcgccgt
     5881 atcccagttg atcgccaagc ccatacagcg caacaatttt accttcaagg ttcaggtcgt
     5941 cgagctgatc ccagacggct tcccagtctt cctggatttc accaaaatcc caggtcggga
     6001 tacccagaat gagcacatcg tactgctcca ttaatttcgg ggagtcgtcc ttgaggttat
     6061 gtaaggtcac cagttctggg ccgataatat cgcggatttt ttctgccgcc atttcggtgt
     6121 aacaggtgct ggaaccgtaa aaaagaccca tattcataac gtaagcttct cgattctgct
     6181 gtggcttttg cagctagtgt atcagaatcg ctttagtcac ggcacaataa ggcataatgg
     6241 atcaaatgag tgaccataag gggcgcaagt gaaacaggat ctggcacgca tcgagcagtt
     6301 tcttgatgct ctgtggctgg aaaaaaatct ggctgaaaat acgttgaacg cttaccgtcg
     6361 cgatctgtca atgatggtgg agtggttgca tcaccgcggg ttgacgctgg cgacggcgca
     6421 aagtgacgat ttgcaggcat tactggcaga acggctggag ggcgggtata aagccaccag
     6481 ctcagcgcgt ttgctgagtg cagtgcgccg attgttccag tatctttatc gcgaaaagtt
     6541 tcgtgaagac gatcccagtg cgcatctcgc ttcaccgaaa ttgccccagc gtttgccaaa
     6601 agatttaagc gaagcgcagg tcgaacgttt attacaggcg ccattaattg atcagccact
     6661 ggagctacgc gataaagcca tgcttgaagt gttgtatgct accgggctgc gtgtctctga
     6721 actggtcggg ctgacaatga gtgatatcag cctgcgtcag ggcgtagtgc gggtcattgg
     6781 taaaggcaac aaagagcgcc tggtaccgtt aggtgaagag gcggtttact ggctggaaac
     6841 ctatctggaa catgggcgtc cgtggctgct gaatggtgtg tcaattgacg tgttgtttcc
     6901 cagccagcgt gcgcagcaga tgacgcgaca gaccttctgg caccgcatta aacattatgc
     6961 cgtgctggcg ggtatcgaca gcgaaaagct gtcaccgcat gtgttgcgtc acgcttttgc
     7021 cactcattta ttaaatcatg gtgcggattt acgcgtggtg cagatgctac tgggccacag
     7081 cgatctttcc accacgcaaa tttatacgca tgtcgctacc gagcgtctgc ggcaacttca
     7141 tcaacagcat cacccgcggg cgtgatgtct gaaaagaacg ggaagattta tgaagaaagg
     7201 ttttatgttg tttactttgt tagcggcgtt ttcaggtttt gctcaggctg atgacgcggc
     7261 aattcaacaa acgttagcca aaatgggcat caaaagcagc gatattcagc ccgcgcccgt
     7321 agctggcatg aagacagttc tgactaacag cggcgtgttg tacatcaccg atgatggtaa
     7381 acatatcatt caggggccaa tgtatgacgt tagcggcacg gctccggtca atgtcaccaa
     7441 taagatgctg ttaaagcagt tgaatgcgct tgaaaaagag atgatcgttt ataaagcgct
     7501 gcaggaaaaa cacgtcatca ccgtgtttac tgatattacc tgtggttact gccacaaact
     7561 gcatgagcaa atggcagact ataacgcgct ggggatcacc gtgcgttatc ttgctttccc
     7621 gcgccagggg ctggacagcg atgcagagaa agaaatgaaa gctatctggt gtgcgaaaga
     7681 taaaaacaaa gcgtttgatg atgtgatggc aggtaaaagc gtcgcaccag ccagctgcga
     7741 cgtggatatt gccgaccatt acgcacttgg cgtccagctt ggcgttagcg gtactccggc
     7801 agttgtgctg agcaatggca cacttgttcc gggttaccag ccgccgaaag agatgaaaga
     7861 attcctcgac gaacaccaaa aaatgaccag cggtaaataa ttcgcgtgaa acaacagata
     7921 caacttcgtc gccgtgcagt cgatgaaacg gcagacttgc ccgctgaatt gcctcccttg
     7981 ctgcgccgtt tatacgccag ccggggcgtg cgcagtgcgc aagaactgga acgcagtgtt
     8041 aaagggatgc tgccctggca gcaactgagc ggcgtcgaaa aggccgttga gatcctttac
     8101 aacgcctttc gcgaaggaac gcggattatt gtggtcggtg atttcgacgc cgacggtgcc
     8161 accagcacgg ctctaagcgt gctggcgatg cgctcgcttg gttgcagcaa tatcgactac
     8221 ctggttccaa accgtttcga agacggttac ggcttaagcc cggaagtggt cgatcaggcc
     8281 catgcccgtg gtgcgcagtt aattgtcacg gtggataacg gtatttcctc ccatgcgggg
     8341 gttgaacacg ctcgctcgtt gggcattccg gttattgtta ccgatcacca tttgccgggc
     8401 gaaacattac ccgcggcgga agcgatcatt aaccctaact tgcgcgactg taatttcccg
     8461 tcgaaatcac tggcaggcgt gggtgtggcg ttttatctga tgctggcgct gcgcaccttt
     8521 ttgcgcgatc agggctggtt tgatgagcgc ggcatcgcaa ttcctaacct ggcagaactg
     8581 ctggatctgg tagcgctggg gacagtggcg gacgtcgtac cgctggacgc taataatcgc
     8641 attctgacct ggcaggggat gagtcgcatc cgtgccggaa agtgccgtcc ggggattaaa
     8701 gcgctgcttg aagtggcaaa ccgtgatgca caaaaactcg ccgccagcga tttaggtttt
     8761 gcgctggggc cacgtctcaa tgctgccgga cgactggacg atatgtccgt cggtgtggcg
     8821 ctcttgctgt gcgacaacat cggcgaagcg cgcgtgctgg caaatgaact cgatgcgcta
     8881 aaccagacgc gaaaagagat cgaacaagga atgcaggttg aagccctgac cctgtgcgag
     8941 aaactggagc gcagccgtga cacgctaccc ggcgggctgg caatgtatca ccccgaatgg
     9001 catcaggggg ttgtcggtat tctggcttcc cgtattaaag agcgttttca ccgtccggtt
     9061 atcgcctttg cgcccgcggg tgatggtacg ctgaaaggtt ctggtcgctc cattcagggg
     9121 ctgcatatgc gtgatgcgct ggagcgatta gacacactct accctggcat gatgctgaag
     9181 tttggcggtc atgcgatggc ggcgggtttg tcgctggaag cggataaatt cgaactcttt
     9241 caacaacggt ttggcgaact ggttactgag tggctggacc cttcgctatt gcaaggcgaa
     9301 gtggtgtcag acggtccgtt aagcccggcc gaaatgacca tggaagtggc gcagctgctg
     9361 cgcgatgctg gcccgtgggg gcagatgttc ccggagccgc tgtttgacgg tcatttccgg
     9421 ctgctgcaac agcggctggt gggcgaacgt catttgaaag tcatggtcga accggtcggc
     9481 ggcggtccgc tgctggatgg tattgctttt aatgtcgata ccgccctctg gccggataac
     9541 ggcgtgcgcg aagtgcaact ggcttacaag ctcgatatca acgagtttcg cggcaaccgc
     9601 agcctgcaaa ttatcatcga caatatctgg ccaatttagc gtcatcttct ctataaaaaa
     9661 gagcgtggat tgggtacaat cccgctctta tcaccgcatt ttgactagct caataaaaga
     9721 aatcagacca tgtttgaaat taatccggta aataatcgca ttcaggacct cacggaacgc
     9781 tccgacgttc ttagggggta tctttgacta cgacgccaag aaagagcgtc tggaagaagt
     9841 aaacgccgag ctggaacagc cggatgtctg gaatgaaccc gaacgcgcac aggcgctggg
     9901 taaagagcgt tcctccctcg aagccgttgt cgacaccctc gaccaaatga aacaggggct
     9961 ggaagatgtt tctggtctgc tggaactggc tgtagaagct gacgacgaag aaacctttaa
    10021 cgaagccgtt gctgaactcg acgccctgga agaaaaactg gcgcagcttg agttccgccg
    10081 tatgttctct ggcgaatatg acagcgccga ctgctacctc gatattcagg cggggtctgg
    10141 cggtacggaa gcacaggact gggcgagcat gcttgagcgt atgtatctgc gctgggcaga
    10201 atcgcgtggt ttcaaaactg aaatcatcga agagtcggaa ggtgaagtgg cgggtattaa
    10261 atccgtgacg atcaaaatct ccggcgatta cgcttacggc tggctgcgta cagaaactgg
    10321 cgttcaccgc ctggtgcgta agagcccgtt tgactccggc ggtcgtcgcc acacgtcgtt
    10381 cagctctgcg tttgtttatc cggaagttga tgatgatatt gatatcgaaa tcaacccggc
    10441 ggatctgcgc attgacgttt atcgcgcgtc cggcgcgggc ggtcagcacg ttaaccgtac
    10501 cgaatctgcg gtgcgtatta cccacatccc gaccgggatc gtgacccagt gccagaacga
    10561 ccgttcccag cacaagaaca aagatcaggc catgaagcag atgaaagcga agctttatga
    10621 actggagatg cagaagaaaa atgccgagaa acaggcgatg gaagataaca aatctgacat
    10681 cggctggggc agccagattc gttcttatgt ccttgatgac tcccgcatta aagatctgcg
    10741 taccggggta gaaactcgca acacgcaggc cgtgctggac ggcagcctgg atcaatttat
    10801 cgaagcaagt ttgaaagcag ggttatgagg aaccaacatg tctgaacaac acgcacaggg
    10861 cgctgacgcg gtagtcgatc ttaacaatga actgaaaacg cgtcgtgaga agctggcgaa
    10921 cctgcgcgag caggggattg ccttcccgaa cgatttccgt cgcgatcata cctctgacca
    10981 attgcacgca gaattcgacg gtaaagagaa cgaagaactg gaagcgctga acatcgaagt
    11041 cgccgttgct ggccgcatga tgacccgccg tattatgggt aaagcgtctt tcgttaccct
    11101 gcaggatgtg ggtggccgca ttcagctgta cgttgcccgt gacgatctgc cggaaggcgt
    11161 ttacaacgag cagttcaaaa aatgggacct cggcgacatc ctcggcgcga aaggtaaact
    11221 gttcaaaacc aaaaccggcg aactgtctat ccactgtacc gagctgcgtc tgctgaccaa
    11281 agcactgcgt ccgctgccgg ataaattcca cggcttgcag gatcaggaag cgcgctatcg
    11341 tcagcgttat ctggatctca tctctaacga tgaatcccgt aacaccttta aagtacgctc
    11401 gcagatcctc tctggtattc gccagttcat ggtgaaccgc ggctttatgg aagttgaaac
    11461 gccgatgatg caggtgatcc caggcggtgc cgctgcgcgt ccgtttatca cccaccataa
    11521 cgcgctggat ctcgacatgt acctgcgtat cgcgccggaa ctgtacctca agcgtctggt
    11581 ggtcggtggc ttcgagcgtg tattcgaaat caaccgtaac ttccgtaacg aaggtatttc
    11641 cgtacgtcat aacccagagt tcaccatgat ggaactctac atggcttacg cagattacaa
    11701 agatctgatc gagctgaccg aatcgctgtt ccgtactctg gcacaggata ttctcggtaa
    11761 gacggaagtg acctacggcg acgtgacgct ggacttcggt aaaccgttcg aaaaactgac
    11821 catgcgtgaa gcgatcaaga aatatcgccc ggaaaccgac atggcggatc tggataactt
    11881 cgactctgcg aaagcgattg ctgaatctat cggtatccac gttgagaaga gctggggtct
    11941 gggccgtatc gttaccgaga tcttcgaaga agtggcagaa gcacatctga ttcagccgac
    12001 cttcattact gaatatccgg cagaagtttc tccgctggcg cgtcgtaacg acattaaccc
    12061 ggaaatcaca gaccgctttg agttcttcat cggtggtcgt gaaatcggta acggctttag
    12121 cgagctgaat gacgcggaag atcaggcgca acgcttcctg gatcaggttg ccgcgaaaga
    12181 cgcaggtgac gacgaagcga tgttctacga tgaagattac gttaccgcac tggaacatgg
    12241 cttaccgccg acagcaggtc tggggattgg catcgaccgt atggtaatgc tgttcaccaa
    12301 cagccatacc atccgcgacg ttattctgtt cccggcgatg cgtccggtaa aataagcatt
    12361 acgttatgct cacaaccccg gcaaatgtcg gggtttttta attgtgctgc gcgaaagcag
    12421 acaacaattt tcttgcttca ctattggctg cctgcatcac catccacgga ctgaacgccc
    12481 acggtgtggc atcaataccg tgtaatacat ctgctaaatc acaccattga taatccatca
    12541 cttcatcatc gttgatctgt aacgcactgg tggtgcgtgc ggcaaatacc ggacacactt
    12601 cattttccac aatgccattc ggatcggtgg cgcgatagcg aaagtcagga tagatagatt
    12661 caggaggcgt aatttccacg ccaagctcat aacggcaacg gcggatcacc gcgtcttcgt
    12721 tgctttctcc cagttgtggg tgcccacaaa ccgagttagt ccacacgcca ggccacgctt
    12781 ttttgctcag tgcgcggcgg gtaactaata attgtccttt ggcattaaac agccaactgg
    12841 agaacgcgag atgtaagcgg gtgtctgccg tgtgtgcggc atacttttcc agcgtacccg
    12901 tgggaactcc ctgtgcattc aataaaatga cgtgttccgt ttgcataatt tctcacatgt
    12961 aattctgatc actcatcatt atagataatt gaagagtgaa atgattaacc aagtgagcgt
    13021 ttatcgacaa ccgcccgttt tgagcggatg ccgacaggta aaaaccattt aaaaaagtag
    13081 catcatatat ttgaagcgac tttcaatggc cattgcaaca cagtcagaaa tgacaggaat
    13141 agagagagta aaaagcaaca gcagactgaa tataacgcgc attaaaaaat tcatattatt
    13201 ctccatgctc atttttcttc agcttgcgca tcagaagcca catacgtact gtcctgactt
    13261 ttaaatcttt atcagagacg gattccttca ccaggtcagc atgatgctgg tagccattaa
    13321 agaagacatt cagcacaacg gcgctaaggg ttgcgagcat aatgccgcta tgcagcaacg
    13381 gttgcagtac ggccggtaac ttagaaaaga aatcgtgaga gagcgtcgga gtcatgccaa
    13441 cgccgagact gatcgccaca atatagaggt tgtaacggtt ggtggtgtag ttacagcgcg
    13501 acagaattcg aatccctgtc gccagtacca tgccgaacat caccagccca gcaccgccca
    13561 gcacaaattg cggaatggag gcgaccagca ccgccatttt tggcaccatg ccaaacagga
    13621 ttaatataat tcccgaagaa atacacaccc agcggctatg aacgcgcgtc acgctaacca
    13681 ggccaacgtt ttgtgaaaat gacgtgtgga ggaagctgtt aaacgttccg cctatcattg
    13741 tccctacgcc atcgacacgc agcccgcgaa taatatcgtg tgaagagagt ttacgaccga
    13801 ctatttcacc cagtgccagg aacatcccca tcgactcgat aaacacgatg attaacacgg
    13861 cagtcatggt cagaatggaa acgggatcga aaatcggcat accaaatgac atcggcgtga
    13921 caatcgcaaa ccatgaagca tcatgtagcc cggataaatt gacttcattc atcatccacg
    13981 aaagtaaaaa gccaaataca atccccagta atacggcgac gttggacata aatcctttcg
    14041 catagcgagt aatgagcaag ataaaaatta agacagcaaa ggagatacct aaataaacgg
    14101 gattaccata ttgcggattc cctttacctc cggcggccca gtcaataccc acctgaatga
    14161 tgctaagccc aatagaagta ataaccacac cggtaaccag tggcgggaat aaaggcatca
    14221 agcgaccaat aaatggcgct aataatgtgg tgataaaacc cgcggcgata gtggcaccaa
    14281 atatccccag caggccgata tccgggttca tcccaatggc tatcattggt gttacagcag
    14341 caaaggtcac cgacataatc accggcaggc ggatccccat aaagcggccg ataccgatac
    14401 attgcaataa tgtgacgatc ccgcagcaaa agagatccga gctaatgagc atcgcaatag
    14461 catcttttga gaggcccagt cggtcaccaa tcataagagg aacagcgact gcacctgcgt
    14521 acatgacaag gacgtgttgc agaccgagta tgatcagctt tcctggtgat aatatgcggt
    14581 caacctcatc agttgggcgg tcttgcccag aagatgaggg aagttgggaa tctatggcgc
    14641 tcatcagtgt ttctcctttc gcccgccaga gtaaaaagca accgtttacg ttaatgaaac
    14701 tgtctaaaca catgctcgtg gagtgccagt ttttgcgagg aaggggagtt agagacagga
    14761 attgcggagg caatcacata atcgtgctta aaccacgaaa atgacaagag aacctgacaa
    14821 tggaattatc tgtaattttc tcatattgat atttcattaa tcattctggc atgtcgcact
    14881 ctcgcattta atcgttttta tctggatagc gctcttttga tcggcaatgt tgaaataatg
    14941 aaagcggcat agattgaggg gcaataattt gaactcagtc gcaaaattat aattaaaacg
    15001 gtcatgctac ctaatatatg cccgtttatt gcatcgcgaa atttgagaag acataactca
//
LOCUS       DAZOYL010000078        14965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZOYL010000078 DAZOYL010000000
VERSION     DAZOYL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.078"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUOY_001757"
     CDS             <1..196
                     /locus_tag="ACHUOY_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0682552.1"
                     /translation="KAILTDGLFALAQKKMPDATLRVLYSHICQIQQRIRFPQLAHFH
                     TCPPYQKFILKLLNNHFPAN"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="ACHUOY_001758"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="ACHUOY_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR0682553.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="ACHUOY_001759"
     CDS             complement(1608..1931)
                     /locus_tag="ACHUOY_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR0682554.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHUOY_001760"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHUOY_001760"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR0682555.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHUOY_001761"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHUOY_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR0682556.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHUOY_001762"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHUOY_001762"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR0682557.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHUOY_001763"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHUOY_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR0682558.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="ACHUOY_001764"
     CDS             7531..8568
                     /locus_tag="ACHUOY_001764"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0682559.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHUOY_001765"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHUOY_001765"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR0682560.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="ACHUOY_001766"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="ACHUOY_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR0682561.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHUOY_001767"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHUOY_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR0682562.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="ACHUOY_001768"
     CDS             10739..11620
                     /locus_tag="ACHUOY_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0682563.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFYIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHUOY_001769"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHUOY_001769"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR0682564.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="ACHUOY_001770"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="ACHUOY_001770"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR0682565.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="ACHUOY_001771"
     CDS             complement(14017..>14965)
                     /locus_tag="ACHUOY_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR0682566.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3783 c         3922 g         3648 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt tatattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAZOYL010000079        14761 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZOYL010000079 DAZOYL010000000
VERSION     DAZOYL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14761
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.079"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHUOY_001772"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHUOY_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR0682567.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="ACHUOY_001773"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="ACHUOY_001773"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR0682568.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="ACHUOY_001774"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="ACHUOY_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR0682569.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHUOY_001775"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHUOY_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR0682570.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="ACHUOY_001776"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="ACHUOY_001776"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR0682571.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="ACHUOY_001777"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="ACHUOY_001777"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR0682572.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="ACHUOY_001778"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="ACHUOY_001778"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR0682573.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="ACHUOY_001779"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="ACHUOY_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR0682574.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHUOY_001780"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHUOY_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR0682575.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="ACHUOY_001781"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="ACHUOY_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR0682576.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="ACHUOY_001782"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="ACHUOY_001782"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR0682577.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="ACHUOY_001783"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="ACHUOY_001783"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0682578.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="ACHUOY_001784"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="ACHUOY_001784"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR0682579.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHUOY_001785"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHUOY_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR0682580.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="ACHUOY_001786"
     CDS             complement(14282..14581)
                     /locus_tag="ACHUOY_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR0682581.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
BASE COUNT         3547 a         3797 c         3975 g         3442 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcgtgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 g
//
LOCUS       DAZOYL010000080        14726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZOYL010000080 DAZOYL010000000
VERSION     DAZOYL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.080"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            383..1672
                     /gene="sdaC"
                     /locus_tag="ACHUOY_001787"
     CDS             383..1672
                     /gene="sdaC"
                     /locus_tag="ACHUOY_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR0682582.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1730..3097
                     /gene="sdaB"
                     /locus_tag="ACHUOY_001788"
     CDS             1730..3097
                     /gene="sdaB"
                     /locus_tag="ACHUOY_001788"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR0682583.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3209..3964
                     /gene="xni"
                     /locus_tag="ACHUOY_001789"
     CDS             3209..3964
                     /gene="xni"
                     /locus_tag="ACHUOY_001789"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR0682584.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4069..5217)
                     /gene="fucO"
                     /locus_tag="ACHUOY_001790"
     CDS             complement(4069..5217)
                     /gene="fucO"
                     /locus_tag="ACHUOY_001790"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR0682585.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5245..5892)
                     /gene="fucA"
                     /locus_tag="ACHUOY_001791"
     CDS             complement(5245..5892)
                     /gene="fucA"
                     /locus_tag="ACHUOY_001791"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR0682586.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6439..7755
                     /gene="fucP"
                     /locus_tag="ACHUOY_001792"
     CDS             6439..7755
                     /gene="fucP"
                     /locus_tag="ACHUOY_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR0682587.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            7788..9563
                     /gene="fucI"
                     /locus_tag="ACHUOY_001793"
                     /pseudo
     CDS             7788..9563
                     /gene="fucI"
                     /locus_tag="ACHUOY_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            9672..11090
                     /gene="fucK"
                     /locus_tag="ACHUOY_001794"
     CDS             9672..11090
                     /gene="fucK"
                     /locus_tag="ACHUOY_001794"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR0682588.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            11092..11514
                     /gene="fucU"
                     /locus_tag="ACHUOY_001795"
     CDS             11092..11514
                     /gene="fucU"
                     /locus_tag="ACHUOY_001795"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR0682589.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11572..12303
                     /gene="fucR"
                     /locus_tag="ACHUOY_001796"
     CDS             11572..12303
                     /gene="fucR"
                     /locus_tag="ACHUOY_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR0682590.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12347..13447)
                     /gene="rlmM"
                     /locus_tag="ACHUOY_001797"
     CDS             complement(12347..13447)
                     /gene="rlmM"
                     /locus_tag="ACHUOY_001797"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR0682591.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13440..13835)
                     /locus_tag="ACHUOY_001798"
     CDS             complement(13440..13835)
                     /locus_tag="ACHUOY_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR0682592.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13854..>14726)
                     /gene="gcvA"
                     /locus_tag="ACHUOY_001799"
     CDS             complement(13854..>14726)
                     /gene="gcvA"
                     /locus_tag="ACHUOY_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR0682593.1"
                     /translation="AAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRS
                     LLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLPSFAIHWLVPRLSSFN
                     SAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCSPLLLT
                     GEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGPIFSHSAMVLQAAIHG
                     QGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAK
                     AAAEQEKFRFRYEQ"
BASE COUNT         3621 a         3868 c         3603 g         3634 t
ORIGIN      
        1 aaccacattt tatcgctatg aaaaagaaat tatcgccatg attaactaaa agtattgatt
       61 ttttcagttc aacctacata tattgcgcgc cccggaagaa gtcagatgtc gtttaatggg
      121 caaatattgc ccttaaattc tcttttactt ttgatttaca gagtaaagcg ttgggataat
      181 ctatcttcca agtagattat tgtatttggg atcaagatca ctgatagata cataacttgt
      241 gtgtatcttt ccgccctcaa attattacgg cggtaaatga ttaagccatc gccgatagac
      301 agatttcatt tttacggtca ggcaccttcc cgagctgaac tggctaaaag ctgaattatt
      361 tgcattcctc caggagaaat agatggaaac gactcaaacc agcacgattg cgtcgaaaga
      421 ctctcgtagt gcctggcgca agacagacac catgtggatg ctgggccttt acggcacggc
      481 aatcggcgcg ggcgtgctgt tcctgccaat caacgccggt gttggcggta tgatcccgct
      541 gatcatcatg gctatccttg cgttcccgat gacatttttt gctcaccgcg gcctgactcg
      601 cttcgtactg tctggtaaaa acccaggcga agacatcacc gaggttgtag aagaacactt
      661 tggtattggc gcaggtaaac tgattaccct gctctacttc ttcgctatct acccgatcct
      721 gctggtttat agcgtggcaa tcaccaatac agttgaaagc ttcatgtctc accagctggg
      781 tatgacacca ccgccgcgtg cgattctgtc gctgatcctg atcgtgggta tgatgaccat
      841 cgttcgcttc ggtgagcaga tgatcgttaa agcaatgagt attctggtat tcccgtttgt
      901 tggcgtactg atgctgctgg ctctgtacct gatcccgcag tggaacggcg cagcactgga
      961 aacgctgtct ctggacactg catctgcaac cggaaacggt ctgtggatga ccctgtggct
     1021 ggcaattccg gttatggtgt tctctttcaa ccactctccg atcatctctt ctttcgccgt
     1081 tgcgaagcgt gaagagtacg gcgatatggc agaacagaaa tgctcgaaga tcctggcatt
     1141 cgcacacatc atgatggtgc tgaccgtaat gttcttcgtc ttcagctgtg tactgagcct
     1201 gactccggca gacctggctg cggctaaaga gcagaacatc tcgattctgt cttacctggc
     1261 taaccacttt aacgcaccgg ttatcgcgtg gatggctccg attatcgcga ttatcgctat
     1321 caccaaatcc ttcctcggcc actacctggg cgcacgtgaa ggcttcaacg gtatggtgat
     1381 taaatctctg cgtggtaaag gtaagtctat cgaaatcaac aagctgaacc gtatcactgc
     1441 gctgttcatg ctggtaacga cctggattgt tgccaccctg aacccgagca tcctgggtat
     1501 gattgaaacc ctgggcggcc caatcatcgc gatgatcctg ttcctgatgc cgatgtacgc
     1561 aattcagaaa gtaccggcaa tgcgtaagta cagcggtcac atcagcaacg tattcgttgt
     1621 cgtgatgggt ctgattgcaa tctccgcaat cttctactct ctgttcagct aagtcctttc
     1681 gcgccgcttt cgggcggcgc ttcctccgtt ttaacacgat gtatttccta tgattagcgt
     1741 attcgatatt ttcaaaatcg gcattggccc ttccagttct cataccgttg gaccaatgaa
     1801 agcgggtaaa caatttaccg acgatctgat tgcccgtaat ctgcttaaag acgtgacccg
     1861 cgtggtggtt gacgtgtacg gctcgctctc tctgaccggt aaaggccacc acactgatat
     1921 cgccattatt atgggcctgg cgggtaacct gccggatacc gtggatatcg attccatccc
     1981 tggttttatt caggatgtga atactcatgg tcgcctgatg ctggcaaacg gtcagcatga
     2041 agtggagttc ccggttgatc agtgcatgaa cttccatgca gacaaccttt ctctgcatga
     2101 aaacggtatg cgcattaccg cgctggcggg cgataaagtc gtttacagcc agacttacta
     2161 ctctattggc ggtggcttta tcgttgatga agagcatttt ggccagcaga atagcgcacc
     2221 ggttgaagtt ccttatccgt acagttcagc agccgatctg caaaaacatt gtcaggaaac
     2281 cgggctgtca ctctctggcc tgatgatgaa aaatgagctg gcgctgcaca gcaaagaaga
     2341 gctggaacag cacctggcga acgtatggga agtcatgcgt ggcggtattg agcgcggtat
     2401 ttccaccgaa ggcgtgttgc ctggcaaact gcgcgttcca cgccgtgctg cggcactacg
     2461 ccggatgctg gtcagccagg ataaaaccac cactgacccg atggcggttg ttgactggat
     2521 caacatgttt gcactggcag tgaacgaaga gaacgctgct ggcggacgcg tggtgactgc
     2581 gccgactaac ggtgcgtgcg ggattatccc ggcagtgctg gcgtactacg acaagttcat
     2641 ccgcgaagtg aacgctaact cactggctcg ttacctgctg gtagccagtg ccattggttc
     2701 tctttataag atgaacgcgt caatttctgg tgctgaagtc ggctgccagg gtgaagttgg
     2761 tgtggcgtgc tcaatggcgg cggctggtct ggcagaactg ttaggtgcaa gcccggcgca
     2821 ggtgtgcatc gcggcggaaa tcgccatgga gcacaacctc ggtctgacgt gtgacccggt
     2881 cgccggacag gtccaggtgc catgcatcga gcgtaacgcc attgcggcag taaaagcggt
     2941 gaacgccgca cgtatggcgc tacgccgtac cagcgagccg cgcgtctgcc tcgataaagt
     3001 tatcgaaacc atgtacgaaa caggtaaaga tatgaacgcc aagtaccgcg aaacctctcg
     3061 cggcggcctg gcaatgaaga tcgttgcctg cgattaatcg ctctccagag gcctcgtttt
     3121 gcgaggcctc ttcccgattt ctcatccagc cgtagcctgt tccggcatcg aatgttaccc
     3181 ttatcgcctg atctttaagg gggttatcgt ggctgttcat ttgcttattg tcgatgcact
     3241 gaatcttatt cgtcgcattc atgccgttca gggatcgccc tgtgtcgaaa cctgccagca
     3301 tgcgctcgat cagctcatta tgcacagcca gccaacccac gcggtcgccg tttttgatga
     3361 tgaaaaccgc agtagcggct ggcgtcatca gcgtttacca gattacaaag cgggtcgacc
     3421 gccaatgccg gaagagttgc acgacgagat gcctgcatta cgcgccgcct ttgagcaacg
     3481 cggcgtcccg tgctggtcaa ccagcggcaa cgaagccgat gacttagccg ccacgctggc
     3541 ggtcaaagtg acacaggccg ggcatcaggc aacgattgtt tcgacagata aaggctactg
     3601 tcagttactt tcaccgacat tacgtattcg tgattacttc cagaaacgtt ggctggatgc
     3661 gccatttatc gataaagaat ttggcgttca accgcagcag ttgcccgatt actggggact
     3721 tgcggggatc agcagttcaa aggtaccggg tgttgcggga atcggaccaa aaagcgccac
     3781 gcagctgctg gtcgagtttc agagtctgga agggatatat gagaatctgg atgcggttgc
     3841 cgaaaagtgg cgcaaaaaat tagaaaccca taaagagatg gcgtttctgt gccgcgatat
     3901 tgcccgctta caaaccgatt tgcatatcga cggcaattta cagcaattgc ggttggtacg
     3961 gtaacggcga gccggatgca aacgattatc aacacattga atttaatgca cttatatacc
     4021 ggataagacg cggctagcgt cgtatccggc attatcacat cagcgcattt accaggcggt
     4081 atggtaaagc tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac
     4141 atcatccagt gccgcctgcg ccagtgccgg aatgtcttcc ttgcgtacac caacatcacg
     4201 caaatgtggc ggaataccga catcacggtt gagagcaaac accgcttcaa cagcggcatt
     4261 acgcgcctct tccaggctca taccttccac tttcacgccc ataacgcgcg cgatatcgcg
     4321 gtacttctca ccggtaaagt cagcgttata gtgcatgaca tgcggtaaca ggatggcgtt
     4381 cgcaacaccg tgtggagtgt tgtaaaacgc gcccagtgga tgcgccatac catgcaccaa
     4441 ccctaaccca acattcgaga agcccatacc cgcaacatac tgcccgagcg ccatttcttc
     4501 tccggcatcc ttatcaccag caaccgatcc tcgcagcgcc ccagcaatga tttcaatcgc
     4561 tttaatgtgc agtgcatcgg ttagcgccca cgcgccacgg gtaatatacc cctcaatagc
     4621 atgagtgagc gcatcgacac ccgtcgcagc tttcagcgct ggaggcatac catccatcat
     4681 gtcagcgtca ataaacgcca cctgcgggat atcatgcgga tcaacgcaaa caaacttgcg
     4741 ccgtttctct tcgtcagtga tcacgtagtt aatggtcact tctgccgcag tacctgctgt
     4801 ggtaggaatt gccagaatcg gtacactggg tttattggtc ggggaaagcc cttccaggct
     4861 acgcacatcg gcaaactccg gattgttgct gataatgcca atcgctttac aagtatcctg
     4921 tggagaacca ccaccaatag cgatcaggta atccgcgccg ctattctgga atacaccgag
     4981 cccttctttg acgacagtaa ttgttgggtt gggcactacg ccgtcgtaaa tcgcccatgc
     5041 cagccctgca gcatccatct tatcggtcac tttcgccacc acgccgcatt gcaccagcgt
     5101 tttatcggtg acgatcagcg ccttctgata accacggcgt ttcacctcat cggttaaagc
     5161 cccaacagca ccccgaccaa accatgccgt ttcgttcaga atcattctgt tagccatcat
     5221 ccttctcctt gttgctttac gaaattactc ttcaatccgt aacccatagg ttttgaattt
     5281 ctccagcact acggcaatct cttcatcgct cagcactggc accgggtccg taatcgccag
     5341 ggtcgccacg taaagttgcg ccagcacttc aacttcatgc gccagccata acgctttttc
     5401 cagattcacc tcacaagcga taagcccatg atgttgtaac aaagttgcct tacgattttt
     5461 gagagccagc gcaacatgtt cagaaagttc gcgtgttcca aaggtcgcat aaggcgcgca
     5521 aggaatagaa ttaccacctg ccgccgcaat catgtagtga atagcgggga tcggtcggtt
     5581 aagaatggaa actgctgtgc aatgaacggc atgattgtga acaaccgcgt tggcatccgg
     5641 tctgctttga taggccgcca tatggaaacg ccattcgctt gaggggagct ttccttcctc
     5701 atgtttaccg ttgccatcaa taaagacaat atgcgactcc gtcagttttt catatggaat
     5761 gcctgtaggc gtaatcagca tcccatcctg ataacgtaca ctgacgttcc ccgctgtccc
     5821 ctggttcagt cccaggcggg tcatttccag gcaagtgtca ataatctgac gagcaagttt
     5881 atttcgttcc attagctacc tctctctgat tcaaaacagg gcaataatgt tgttcctttc
     5941 acactattga attagccgtt taattaccca ccatcttctt cctgattaac aagaaagaaa
     6001 ttcacaagct tatattttgt gacctagctc aactaatcac agtaaataac tgcaagttct
     6061 ctttttataa ccccattaaa aatgaccgct cttaaaaata tttatcaaaa cggtcatttt
     6121 tctattcacc caagccagga atgcccgttt tcggcataaa caattaatac agtcatctgg
     6181 tttgtgtttt ttatgattta ttttctgaaa cgggcatgaa atttcgatta ttaaagtgat
     6241 ggtagtcaca taaagtcacc ttccagctaa taagtgtgac cgccgtcata ttacagagcg
     6301 ttttttattt gaaaatgaat ccatgagttc atttcagaca ggcaaatatt cactgatatg
     6361 aagcccgaac tcgctggttt tgcacttttg aaaacataac cgattacgtg cttaagcttc
     6421 tgaacctaag aggatgctat gggaaacaca tcaatacaaa cgcagagtta ccgtgcggta
     6481 gataaagatg cagggcaaag cagaagttac attattccat tcgcgctgct gtgctcactg
     6541 ttttttcttt gggcggtagc caataacctt aacgacattt tattacctca attccagcag
     6601 gcttttacgc tgacaaattt ccaggctggc ctgatccaat cggcctttta ctttggttat
     6661 ttcattatcc caattcctgc tgggatattg atgaaaaaac tcagttataa agcaggaatt
     6721 attactgggt tatttttata tgccctgggc gctgcattat tctggcccgc tgcagaaata
     6781 atgaactaca ccttattttt agttggccta tttattattg cagccggatt aggttgtctg
     6841 gaaactgccg caaacccttt tgttacggta ttagggccgg aaagcagtgg tcacttccgc
     6901 ttaaatcttg cgcaaacatt taactcgttt ggcgcaatta tcgcggttgt ctttgggcaa
     6961 agacttattt tgtctaacgt gccacatcaa tcgcaagacg ttctcgataa aatgtctcca
     7021 gagcaattga gtgcgtataa acacagcctg gtattatcgg tacagacacc ttatatgatc
     7081 atcgtggcta tcgtgttact ggtcgccctg ctgatcatgc tgacgaaatt cccggcattg
     7141 cagagtgata atcacagtga cgccaaacaa ggatcgttct ccgcatcgct ttctcgccta
     7201 gcgcgtattc gccactggcg ctgggcagta ttagcgcaat tctgctacgt tggcgcacaa
     7261 acggcctgct ggagctattt gattcgctac gctgtagaag aaattccagg tatgactgca
     7321 ggctttgccg ctaactattt aaccggaacc atggtgtgct tctttattgg tcgtttcacc
     7381 ggtacctggc tcatcagtcg cttcgcacca cacaaagtcc tggccgccta cgcattaatc
     7441 gctatggcac tgtgcctgat ctcagccttc gctggcggtc atgtgggctt aatagccctg
     7501 actttatgca gcgcctttat gtcgattcag tacccaacaa tcttctcgct gggcattaag
     7561 aatctcggcc aggacaccaa atacggttcg tccttcatcg ttatgaccat tattggcggc
     7621 ggtattgtca ctccggtcat gggttttgtc agtgacgcgg cgggcaacat ccccactgct
     7681 gaactgatcc ccacactctg cttcgcggtc atctttatct ttgcccgttt ccgctctcaa
     7741 acggcaacta actgaacata ttttccgaat aaagtgagga atctgtaatg aaaaaaatca
     7801 gcttaccgaa aattggtatc cgcccggtta ttgacggtcg tcgcatgggt gttcgtgagt
     7861 cgcttgaaga acaaacaatg aatatggcga aagctacggc cgcactgctg accgagaaac
     7921 tgcgccatgc ctgcggagct gccgtcgagt gtgtcatttc cgatacctgt atcgcgggta
     7981 tggctgaagc cgctgcttgc gaagaaaaat tcagcagcta gaatgtaggc ctcaccatta
     8041 cggtaacgcc ttgctggtgc tatggcagtg aaaccatcga catggatcca acccgcccga
     8101 aagccatttg gggctttaac ggcactgaac gtcccggcgc tgtttacctg gcagcggctc
     8161 tggcagctca cagccagaaa ggcatcccgg cattctccat ttacggtcat gacgttcagg
     8221 atgccgatga cacatcgatt cctgccgatg ttgaagaaaa actgctgcgc tttgcccgcg
     8281 ccggtttggc cgtcgccagc atgaaaggta aaagctatct gtcgctgggc ggcgtttcga
     8341 tgggtatcgc cggttctatt gttgatcaca acttctttga atcctggctg ggaatgaaag
     8401 tccaggcggt ggatatgacc gaactgcgtc gccgtatcga tcagaagatt tacgacgaag
     8461 ccgaactgga aatggcactg acctgggctg ataaaaactt ccgctatggc gaagatgaaa
     8521 ataacaaaca gtatcaacgc aatgccgagc aaagccgcgc agttctgcgc gaaagtttac
     8581 tgatggcgat gtgtatccgc gacatgatgc aaggcaacag caaactggcc gatattggtc
     8641 gcgtggaaga atcacttggc tacaacgcca tcgctgcggg cttccagggg caacgtcact
     8701 ggaccgatca atatcccaat ggtgacaccg ccgaagcgat cctcaacagt tcatttgact
     8761 ggaatggcgt gcgcgaaccc tttgtcgtgg cgaccgaaaa cgacagtctt aacggcgtgg
     8821 caatgctaat gggtcaccag ctcaccggca ccgctcaggt atttgccgat gtgcgtacct
     8881 actggtcacc agaagcaatt gagcgtgtaa cggggcataa actcgatgga ctggcagaac
     8941 acggcatcat ccatttgatc aactcaggtt ctgctgcgct ggacggttcc tgtaaacaac
     9001 gcgacagcga aggtaacccg acgatgaagc cacactggga aatctctcag caagaggctg
     9061 acgcttgcct cgccgctacc gaatggtgcc cggcgatcca cgaatacttc cgtggcggcg
     9121 gttactcttc ccgcttcctt accgaaggcg gcgtcccgtt caccatgact cgtgtcaaca
     9181 tcatcaaagg cctgggatcg gtactgcaaa tcgcggaagg ctggagcgtg gaattgccga
     9241 aggatgtgca tgacatcctc aacaaacgca ccaactcaac ctggccaacc acctggtttg
     9301 caccgcgcct caccggtaaa gggccgttta cggatgtgta ctcggtaatg gcgaactggg
     9361 gcgctaacca tggggttctg accatcggcc acgttggcgc agactttatc actctcgcct
     9421 ccatgctgcg tatcccggta tgtatgcaca acgttgaaga gaccaaagtg tatcgtcctt
     9481 ctgcctgggc tgcgcacggc atggatattg aaggccagga ttaccgcgct tgccagaact
     9541 acggtccgtt gtacaagcgt taatacatta ctccctgatg tgatgcccgg tcgctccggc
     9601 taccgggcct gaacaagcaa gagtggttag ccggataagc aatgttatcc ggctatattg
     9661 caggagcgat tatgaaacaa gaagttatcc tggtactcga ctgtggcgcg accaatgtca
     9721 gggccatcgc ggttaatcgg cagggaaaaa ttgttgcccg cgcctcaacg cctaatgcca
     9781 gcgatatcgc gatggaaaac aacacctggc accagtggtc tttagacgcc attttgcaac
     9841 gctttgctga ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta
     9901 tcgccgtcac cacctttggt gtggatggcg ctctggtaga taagcaaggc aatctgctct
     9961 atccgatcat tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacagt
    10021 tgatctccgc acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt
    10081 tatataagtt ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct
    10141 ggctctttat ttcgtcgctg attaaccacc gtttaaccgg cgaattcact actgatatca
    10201 cgatggccgg aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt
    10261 tacaagccac cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga
    10321 ttggtacgct acagaacagc gccgcagcaa tgctcggctt acccgttggc ataccggtga
    10381 tttccgcagg tcacgatacc cagttcgccc tttttggcgc tggtgccgac caaaatgaac
    10441 ccgtgctctc ttccggtaca tgggaaattt taatggtccg tagcgcccag gttgatactt
    10501 cgctgttaag tcagtacgcc gattctacct gcgaactgga tagccaggca gggttgtata
    10561 acccaggtat gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga
    10621 cggctgaaac accctggcaa atgttgattg aagaagctcg tctgatcgca cctggcgcgg
    10681 atggtgtgaa aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga
    10741 cgcttaatac cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat
    10801 tacagcgcaa tctacagatg ctggaaaaaa tcgggcactt taaagcctct gaattattgt
    10861 tagtcggtgg aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata
    10921 ttccggtaaa agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt
    10981 ggtatggcgt aggggaattt aacagcccgg aagaagcccg tgcacagatt cattatcagt
    11041 accgttattt ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa
    11101 acaatttcgc cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga
    11161 gatgaaatta ttttttccga tgctcacttt cccgcccatt cgatgggacc gcaggtgatc
    11221 cgcgctgatg gcctgttggt gagcgacttg ctccaagcga ttatcccatt atttgagctg
    11281 gacagttatg ccccgccgct ggtgatgatg gcagcggtag aaggtgacac tctcgatcct
    11341 gaagtagaac gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc
    11401 cgcatcaatc gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca
    11461 ggcgaacgag cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat
    11521 atcggtacgc ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg
    11581 gcacgccagc aagcgatagt cgacctgctg ctgaaccata ccagcctgac cacggaagct
    11641 ctctctgaac agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag
    11701 acgcagggta aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa
    11761 gacagtggcg atccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc
    11821 gcgcgcgagg cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca
    11881 acttgctggt atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc
    11941 catccgattt gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc
    12001 acacttgagc gcaaatatgg ctgttacgtc aatccctcgc tgatttccca acttaaatcg
    12061 ctggaaatcg atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg
    12121 gactccaatg cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg
    12181 ttgttattga ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggcatctg
    12241 gatgaggtaa cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc
    12301 tgacggaaag ggtagcaggc cgaagacgac gccccggcct tgcctgttat cgctcgtcgc
    12361 gacgaccacc caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt
    12421 gatacaactg ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa
    12481 tatacgccag attgtgtgat acttcttcgt agcgtttttt catcggcagt ttgagattga
    12541 aaatggtttc acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttag
    12601 ccggtttttc aaccatatcg cataccatcc aggagatatt gctgcgtgtc ggacggaatt
    12661 tgaaaccgtc ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg
    12721 ggccgttgtc gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc
    12781 cagggcaagc gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat
    12841 ccgcaggaat aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcct
    12901 ctgccggaaa tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg
    12961 agtaaccggt atagcagcag cctggtgcaa taaagaatac atgcacaacc ggacgcttcg
    13021 gcgtttcata gttcgccagc accccggcat cgcgcaaggc agcgcgtagc ggcacggtga
    13081 atttacggca gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca
    13141 gttcaccgcc cttctctact acgccctgca acatgtcgac aatgggggta atacgatctt
    13201 ctggcggcaa atgctgcaag agttccccca ccacaaacca ctggcgggca aaaattaatg
    13261 aactgaacgg cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa
    13321 tgacataacc cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt
    13381 tatcggtaat ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct
    13441 tattcatgac ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag
    13501 cccgcgagga agctcacgcc gccaaccggt gtgacaaatg cccacaaacg cagatgggac
    13561 agcgccaggc aataaaggct gccgctgaac aacaccgtgc ctaacgcgag gaaaacgcta
    13621 ctccagtaaa accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc
    13681 agcgtatgaa acgcctggta ttcgaggccg gtctggatcc agcccatctc aacggccccc
    13741 atggttttac ttaacacatg cgcgccaaaa gcgcccagag ccacaaaaat gaagccgcta
    13801 atggcggcga aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt
    13861 tcataacgaa agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga
    13921 aaggcggcta ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcgttt
    13981 ttactgacca gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc
    14041 gccatcacgt tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg
    14101 gcgctatgac taaaaattgg cccttgctga acgttgatat gatttaaccc caactgtcgg
    14161 gtatatgtct gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct
    14221 tccggggtct tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa
    14281 tattcggcgt acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc
    14341 gccacatcaa catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg
    14401 tcaattcccg gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg
    14461 aaactgggga gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc
    14521 gtcgcttcgg ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc
    14581 tcggtcagca ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca
    14641 agagacttga tttgatgact tactgcggcc tgggtcacga aaagctcttc tgctgcgcga
    14701 gtgaaactta aatggcgtgc tgcggc
//
LOCUS       DAZOYL010000081        14725 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZOYL010000081 DAZOYL010000000
VERSION     DAZOYL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.081"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..83)
                     /gene="hypA"
                     /locus_tag="ACHUOY_001800"
     CDS             complement(<1..83)
                     /gene="hypA"
                     /locus_tag="ACHUOY_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="plays a role in hydrogenase nickel cofactor
                     insertion; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0682594.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTA"
     gene            complement(76..564)
                     /gene="hybE"
                     /locus_tag="ACHUOY_001801"
     CDS             complement(76..564)
                     /gene="hybE"
                     /locus_tag="ACHUOY_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR0682595.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(557..1051)
                     /locus_tag="ACHUOY_001802"
     CDS             complement(557..1051)
                     /locus_tag="ACHUOY_001802"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR0682596.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1051..2754)
                     /gene="hybC"
                     /locus_tag="ACHUOY_001803"
     CDS             complement(1051..2754)
                     /gene="hybC"
                     /locus_tag="ACHUOY_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR0682597.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(2751..3929)
                     /gene="hybB"
                     /locus_tag="ACHUOY_001804"
     CDS             complement(2751..3929)
                     /gene="hybB"
                     /locus_tag="ACHUOY_001804"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR0682598.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(3919..4905)
                     /gene="hybA"
                     /locus_tag="ACHUOY_001805"
     CDS             complement(3919..4905)
                     /gene="hybA"
                     /locus_tag="ACHUOY_001805"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR0682599.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(4908..6026)
                     /gene="hybO"
                     /locus_tag="ACHUOY_001806"
     CDS             complement(4908..6026)
                     /gene="hybO"
                     /locus_tag="ACHUOY_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR0682600.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6215..6502)
                     /gene="yghW"
                     /locus_tag="ACHUOY_001807"
     CDS             complement(6215..6502)
                     /gene="yghW"
                     /locus_tag="ACHUOY_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR0682601.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(6621..7508)
                     /gene="yghX"
                     /locus_tag="ACHUOY_001808"
     CDS             complement(6621..7508)
                     /gene="yghX"
                     /locus_tag="ACHUOY_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR0682602.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            7665..8705
                     /gene="gpr"
                     /locus_tag="ACHUOY_001809"
     CDS             7665..8705
                     /gene="gpr"
                     /locus_tag="ACHUOY_001809"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR0682603.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(8745..9239)
                     /locus_tag="ACHUOY_001810"
     CDS             complement(8745..9239)
                     /locus_tag="ACHUOY_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR0682604.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9430..10314
                     /gene="yghA"
                     /locus_tag="ACHUOY_001811"
     CDS             9430..10314
                     /gene="yghA"
                     /locus_tag="ACHUOY_001811"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0682605.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(10474..10899)
                     /gene="exbD"
                     /locus_tag="ACHUOY_001812"
     CDS             complement(10474..10899)
                     /gene="exbD"
                     /locus_tag="ACHUOY_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR0682606.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(10906..11640)
                     /gene="exbB"
                     /locus_tag="ACHUOY_001813"
     CDS             complement(10906..11640)
                     /gene="exbB"
                     /locus_tag="ACHUOY_001813"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0682607.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            11892..13079
                     /gene="metC"
                     /locus_tag="ACHUOY_001814"
     CDS             11892..13079
                     /gene="metC"
                     /locus_tag="ACHUOY_001814"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR0682608.1"
                     /translation="MTDKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13219..13878
                     /gene="yghB"
                     /locus_tag="ACHUOY_001815"
     CDS             13219..13878
                     /gene="yghB"
                     /locus_tag="ACHUOY_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR0682609.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(13918..>14725)
                     /gene="yqhC"
                     /locus_tag="ACHUOY_001816"
     CDS             complement(13918..>14725)
                     /gene="yqhC"
                     /locus_tag="ACHUOY_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR0682610.1"
                     /translation="RLLYGETPFARTPVMYEPGIIILFSGHKIGYINERVFRYDANEY
                     LLLTVPLPFECETYATSEVPLAGLRLNVDILQLQELLMDIGEDEHFQPSMAASGINSA
                     TLSEEILCAAERLLDVMERPLDARILGKQIIREILYYVLTGPCGGALLALVSRQTHFS
                     LISRVLKRIENKYTENLSVEQLAAEANMSVSAFHHNFKSVTSTSPLQYLKNYRLHKAR
                     MMIIHDGMKASAAAMRVDYESASQFSREFKRYFGVTPGEDAARMRAMQGN"
BASE COUNT         3616 a         3949 c         3699 g         3461 t
ORIGIN      
        1 gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc
       61 tggcaaagag acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg
      121 ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc
      181 atccgtcagg cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga
      241 acaggagaga tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tagtgccata
      301 cggcagttgc agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc
      361 ggggaagata actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc
      421 gaacagcgta aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg
      481 catcgaacgc cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg
      541 gaaacctgct atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt
      601 cgcgcagcgc agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg
      661 ttaagccgat gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg
      721 gaaactcacc ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag
      781 agattttgtt ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg
      841 gcgtgttctt tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt
      901 cgccaagcag ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa
      961 tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa
     1021 tattgccgac ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt
     1081 gccgtcggca tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt
     1141 acgcaccact tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc
     1201 gtaaggaccg acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac
     1261 tgcctggtag ttactgataa taccgtcttt aataaccatc cagtgagaga gcataccgcg
     1321 cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac
     1381 aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt gattttgcag
     1441 gatatcctgc aattcgcagc agtgaacggt acgaccaata atacggccca gcgtggagtg
     1501 cagctgcgcc acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt
     1561 cagtttgttc tgggtagatt cgcgacctgc cgccagtttc accagcatat tagccagcgg
     1621 acccacttct accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc
     1681 gtcagaccaa ccatcataag ccggaatggt ggtaccttcc cacggtgcct gcggcgcttc
     1741 gtctttatac caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga
     1801 atgagaagtg atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa
     1861 caggaagctg ccgtttttac tgtcggtcgg gaattccggc acgctcaggt agttcaccgc
     1921 acctttaccg cgcgtcagcc attccgggta gaacgcggca ataactgcgg tatcaacctt
     1981 ataaacctgt tcaacaaagt cgctcagctt gtcgatgaaa gacttgatgt acatcaggcg
     2041 ctcaaggttc agcacgccca aaccgtcgag gttgatcggg ttcgcgacac cacctaccgc
     2101 caggttctga atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg
     2161 ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag
     2221 tttcatcgcc gggtgacccc agtagccatt ggcgaaaata cccaactgac cgctggcaac
     2281 cagatctttg atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt
     2341 cgaaacgcct ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat
     2401 gtccacccag tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg
     2461 cgcagccaga atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc
     2521 actttctgcc gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg
     2581 ttgcacaatc atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg
     2641 ccacatggta ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat
     2701 gcgtaaatgc ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg
     2761 ctcgcctcat gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga
     2821 atgagtacga tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct
     2881 tcccaggtcg ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc
     2941 agacgccagg ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa
     3001 tcattacgca gcttcgccac acgcagaacg accagcggga agagcatcag caggacttca
     3061 atccagaaca tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag
     3121 ataagctcgc caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc
     3181 agcttgacga acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc
     3241 gaaccttcaa agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc
     3301 ggcaacattt catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt
     3361 gaccccattg aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac
     3421 ataaccttgt tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga
     3481 gcaaactcca gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt
     3541 accgagttca cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc
     3601 acgtcgatag tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc
     3661 ggacgcacca gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc
     3721 cagccgccac aggcaaagcc ggtgccaatc agcaggtcaa acgcgatcca cacgccccac
     3781 gggaagccgc cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc
     3841 aggagcatac agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg
     3901 ccgcccagcg gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt
     3961 ggtgttgcga cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc
     4021 tttatacagg gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt
     4081 cggcaggtcg agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc
     4141 ctctttctcg ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc
     4201 agatttcagc gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt
     4261 tttcgcctcc gccatcagct cttcacgcgt accgaaaatc accgcgcccg ccgggcacac
     4321 ttctacgcag ccaggcagac cgcctttatc gagacgttcc acacctttct ggttgcacag
     4381 ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtacgg
     4441 acaggcgacc atgcagtaac ggcaaccggt gcacacgtct ttgtcgtaat ggacaatgcc
     4501 ggttttcgga tcttttttca gtgcagagac cgggcacaca gagacacagt tcggatcgac
     4561 gcagtgcata cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac
     4621 ccctgtgccg ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt
     4681 gttcgaccag gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt
     4741 ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc
     4801 cggaattggc gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag
     4861 caatgccccg caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt
     4921 gagtcagcgt tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca
     4981 ccggcaacca gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg
     5041 ccctctttag cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca
     5101 agctgatgga tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga
     5161 ccaatcgcta ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg
     5221 taagtttctg gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca
     5281 tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg
     5341 tgaatcagac ggccataggc gaaggtcgga cggtttttgt catccagttt cggcggtttg
     5401 ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc
     5461 ggaatattga taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg
     5521 ttaactccag ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct
     5581 gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa
     5641 taaataccgt tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg
     5701 tacttctcaa gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc
     5761 acttcgtgat actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt
     5821 gcacgaagca gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc
     5881 ggacgctgcg ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc
     5941 atggtggcgg ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa
     6001 tggatgaggg tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata
     6061 ttttgcctca atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta
     6121 tggttatcga tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg
     6181 taattaatta aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca
     6241 taaaaaagcg caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt
     6301 tatccagtcc atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc
     6361 cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac
     6421 agaattttgt cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc
     6481 ctttaccaaa atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga
     6541 tattttaggc aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa
     6601 tccagtaaga gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc
     6661 caggcaagat cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga
     6721 ttaacccccg gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg
     6781 taagcaggcc agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc
     6841 gcttcaatct tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag
     6901 gccagttccg gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg
     6961 gtaataccca ctttgcctgc cgcttgtgga tagcgttgca taaactcaat tgcggcaaag
     7021 aaatcattca tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca
     7081 tttcccggat aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc
     7141 ttcgccactc gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc
     7201 accaccacgg ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc
     7261 tcgccgtgac catttggcga aggatacatg atgtactcag caacaatctc cgggtcggta
     7321 aactctacct gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc
     7381 gtcatcccgc ccaccgcata cttcgccgca aggttgagga actcacgttt cgaaattttc
     7441 ccgtgagcgt aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa
     7501 cgcggcatcg ttgcactcct cagttggtgt ttttttaagc aaagcataag cacgtgtttt
     7561 tgcccagttt ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa
     7621 actagaaaca ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga
     7681 atcccgaacg ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc
     7741 ccgcgttatc gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc
     7801 gtgcgatcct gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt
     7861 acgggccgcc tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg
     7921 ccgcttatcg cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc
     7981 cttacggctc tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc
     8041 gtatagggct tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga
     8101 tggaagaaac cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg
     8161 ggatctcctc ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga
     8221 aaattccgct tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa
     8281 gcggcctgct ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg
     8341 ctcagggatt gctgaccgga aaatatctca acggcattcc gcaagattca cggatgcatc
     8401 gtgaagggaa taaagttcgt ggtctgacgc cgaaaatgct taccgaagcc aacctcaaca
     8461 gcctgcgctt attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt
     8521 taagctggtt gctgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg
     8581 agcaactaga ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg
     8641 cgcagattga tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata
     8701 aatgacctgt taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga
     8761 gtcagtcggt caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg
     8821 acgtaccaca tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtaag
     8881 tgaatggaag aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc
     8941 attttcccca gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa
     9001 ttctcataac cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc
     9061 agcgacagca acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg
     9121 tgaataatct cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag
     9181 tacacggggg caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata
     9241 cattcctgtc gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca
     9301 acagcaaatc aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc
     9361 ctggttccct tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg
     9421 agaaaaacga tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc
     9481 aaacagaaac aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc
     9541 gagaaaacct atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc
     9601 gattccggaa taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg
     9661 atcagttatc ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa
     9721 tgcggacgca aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg
     9781 ctggttcacg aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg
     9841 aaacaggttg ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt
     9901 gccattaacg ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa
     9961 ggtgcaagta tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg
    10021 gactatgcgg ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc
    10081 gcggagaaag gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa
    10141 atttccggcg gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag
    10201 cgtgcggggc aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg
    10261 agctacgtca ccgcagaagt gcacggcgtg tgcggcggcg agcatttagg ttaaaaaaat
    10321 gcccggttgt gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa
    10381 aacgccttat ccggcctaca aacgcacgaa gattcaacat gttgcaatag attttgtagg
    10441 cctgatgagc gtagcgcatc aggcaattct actttacttc gctttggcgg tttcttcgcc
    10501 gaccagacct atcttcaggt aacccgcctg atgcagcgta tccattacct tcatcaacgt
    10561 ctcgtaatcg acggttttgt ccgctcggaa gaagatggtg gtgtctttct tgccttcggt
    10621 taacgcattc aacgccgtaa tcattgtttc atcggtgacc ggatcgttac cgataaacat
    10681 cgagttgtct gccttcaccg acagataaac cggtttttcc gggcgcggct gcggcgtgct
    10741 ggtagaagca ggcaagttca ccttcacatc taccgtcgct aacggtgccg ccaccataaa
    10801 gataatcagc agaaccaaca tcacgtcgat aaacggcgtc acgttgatat catgcatttc
    10861 gccgttatcg tcgaggtttt cgttaagatg cattgccatc ggacattatc ctgcgcgtaa
    10921 tttttgtgcg acacgaaccg gatgcgcagc ggcgctggct tccagatcca ggtcacggct
    10981 ttgcagcaac aatacctgcg ctgcaacatc acccagcatc gctttaaagc cgccaatctg
    11041 gcgtgcaaat acgttataga taacgaccgc aggaatcgct gccacgaggc cgattgccgt
    11101 tgctaacaga gcttctgcga tacccggcgc aacgactgcc aggttagtgg tctgcgtttg
    11161 cgcgatacca ataaagctgt tcatgatgcc ccagaccgta ccaaacagac caacgaacgg
    11221 agaaatcgcg ccgatggttg ccagatagcc gttaccgcgg cccatttgac gacccactgc
    11281 ggcgacccga cgctccagac ggaagctggt acgttcttta ataccttcgt tatcgtcgct
    11341 gccttctgac agttccagct cattctgcgc ttcattgagc aaatgcaggc ttaagctttt
    11401 gctaccaaaa tcagcggcga tatcgttggc ctggtttaag gaacgcgctt cagccagcag
    11461 ttgctgctcg cgcttaaggc gacgcttctg attgaagaac tctacgctct tactgaagaa
    11521 gattgcccaa gtgactacgg aggccaaaat aagcccaatc atcacgcact taacgacaat
    11581 atcggcgtgc tgatacatac cccagacgga aaggtccgtc tgcatcaaat tattacccac
    11641 tgtgtatctc caggacgcaa gtcacaaaat ctgcgcataa taatatcaaa atgacgtcga
    11701 attgatagtc gttctcatta ctatttgcat actgccgtac ctttgctttc ttttccttgc
    11761 gtttacgcag taaaaaagtc accagcacgc catttgcgaa aattttctgc tttatgccaa
    11821 ttcttcagga tgcgcccgcg aatattcatg ctagtttaga catccagacg tataaaaaca
    11881 ggaatcccga gatgacggac aaaaagcttg atactcaact ggtgaatgca ggacgcagca
    11941 aaaaatacac tctcggcgcg gtaaatagcg tgattcagcg cgcttcttcg ctggtctttg
    12001 actgtgtgga agccaaaaag cacgcgacgc gcaatcgcgc caatggcgag ttgttctatg
    12061 gacggcgcgg aacgttaacc catttctcct tacaacaagc gatgtgtgaa ctggaaggtg
    12121 gcgcaggctg cgtgctattt ccctgcgggg cggcagcggt tgctaattcc attcttgctt
    12181 ttgtcgaaca gggcgatcat gtgttgatga ccaacaccgc ctatgaaccg agtcaggatt
    12241 tctgtagcaa aatcctcagc aaactgggcg taacgacatc gtggtttgat ccgctgattg
    12301 gtgccgatat cgttaagcat ctgcagccaa acactaaaat cgtgtttctg gaatcgccag
    12361 gctccatcac catggaagtc cacgacgttc cggcgattgt tgccgccgta cgcagtgtgg
    12421 tgccggatgc catcattatg atcgacaaca cctgggcagc cggtgtgctg tttaaggcgc
    12481 tggactttgg catcgatgtt tctattcaag ccgccaccaa atatctggtt gggcattcag
    12541 atgcgatgat tggcactgcc gtatgcaatg cccgttgctg ggagcagcta cgggaaaacg
    12601 cctatctgat gggccagatg gtcgatgccg ataccgccta tataaccagc cgtggcctgc
    12661 gcacattagg tgtgcgtttg cgtcaacatc atgaaagcag tctgaaagtg gctgaatggc
    12721 tggctgaaca tccgcaagta gcgcgagtta accaccctgc tctgcctggc agtaaaggcc
    12781 acgaattctg gaaacgagac tttacaggca gcagcgggct attttccttt gtgcttaaga
    12841 aaaaactcag taatgaagag ctggcgaact atctggataa cttcagttta ttcagcatgg
    12901 cctactcgtg gggcgggtat gaatcgttga tcctggcgaa tcaaccagaa catatcgccg
    12961 ccattcgccc acaaggcgag atcgatttta gcgggacctt gattcgcctg catattggtc
    13021 tggaagatgt cgacgatctg attgccgatc tggacgccgg ttttgcgcga attgtataac
    13081 attgccactt ttggacaatt ttgcagacat ttaattgtga aaagtcttaa attgttgcgt
    13141 ccgggatcaa ggcgtcccgg acgaatcagg agtacaatag gcagataaag gcttaaacgc
    13201 tgttccacag gaaagtccat ggctgttatt caagatatca tcgctgcgct ctggcaacac
    13261 gactttgccg cgctggcgga tcctcatatt gttagcgttg tttactttgt catgtttgcc
    13321 acgctgtttt tagaaaacgg cctgctgccc gcctcatttt tgccaggcga cagcttgttg
    13381 atactggcag gcgcattgat tgcccaaggg gttatggatt ttctgcctac gattgcgatt
    13441 ctgaccgccg cagcaagtct gggctgctgg ctaagttata ttcaggggcg ctggttaggg
    13501 aataccaaaa cggtgaaagg ctggctggca cagcttcctg ctaaatatca ccagcgcgcc
    13561 acctgcatgt ttgaccgcca cggtctgctg gcgctgctgg ctggacgttt tcttgcattt
    13621 gtccgtacgc tgctgccaac catggcggga atttccggtc tgccaaaccg ccgcttccag
    13681 tttttcaact ggttaagtgg attgctgtgg gtcagcgtgg taaccagttt tggctatgcc
    13741 ttaagtatga ttccgttcgt taaacgccat gaagatcagg taatgacgtt cctgatgatc
    13801 ctgccaattg ccttgttaac cgctggcttg ttaggcacgc tgtttgtagt gattaaaaaa
    13861 aaatactgta acgcctgacg attttccccg ttcccggctg ctgtaccggg aacgtattta
    13921 attcccctgc atcgcccgca ttcttgccgc atcttccccc ggcgtcacac cgaagtaacg
    13981 tttaaactca cggctaaatt gcgatgcgct ttcatagtcg acgcgcatcg ctgctgcgct
    14041 ggccttcatg ccgtcatgga tgatcatcat ccgcgcctta tgcagacggt aattcttcaa
    14101 atactgcaac ggcgaggtgc tggtgacaga cttaaaatta tgatggaacg ccgatacgct
    14161 catgttggct tctgccgcca gttgctcgac gctcaggttt tcggtgtatt tattctcaat
    14221 ccgtttcagc acgcggctaa tcagactgaa gtgagtctgg cgactgacca gcgccagtaa
    14281 cgcgccgccg caaggtccgg tcagcacgta gtacagaatt tcgcggatga tctgtttgcc
    14341 gagaatacgc gcatccagtg gtcgctccat cacgtcgagt aaccgctccg ccgcgcataa
    14401 aatctcttct gataacgtgg cggagttaat cccgctggct gccatcgacg gctggaaatg
    14461 ctcatcttcg ccaatgtcca tcaacagttc ctgtaactgc aaaatatcga cattgagacg
    14521 caatcctgcc agcggcacct ctgacgtggc ataggtttcg cactcaaacg gcaacggcac
    14581 cgtcagcagc aggtattcat tggcatcata acgaaacacg cgttcattga tataaccgat
    14641 tttatgcccg gaaaagagaa ttatgatgcc aggctcgtac atcaccggtg tacgtgcgaa
    14701 aggcgtctcg ccatacaaca aacgc
//
LOCUS       DAZOYL010000082        14540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZOYL010000082 DAZOYL010000000
VERSION     DAZOYL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.082"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            132..1076
                     /gene="ydgH"
                     /locus_tag="ACHUOY_001817"
     CDS             132..1076
                     /gene="ydgH"
                     /locus_tag="ACHUOY_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR0682611.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            1262..2644
                     /locus_tag="ACHUOY_001818"
     CDS             1262..2644
                     /locus_tag="ACHUOY_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0682612.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            2681..3403
                     /gene="folM"
                     /locus_tag="ACHUOY_001819"
     CDS             2681..3403
                     /gene="folM"
                     /locus_tag="ACHUOY_001819"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR0682613.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(3400..3735)
                     /locus_tag="ACHUOY_001820"
     CDS             complement(3400..3735)
                     /locus_tag="ACHUOY_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR0682614.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            3864..4583
                     /gene="rstA"
                     /locus_tag="ACHUOY_001821"
     CDS             3864..4583
                     /gene="rstA"
                     /locus_tag="ACHUOY_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR0682615.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            4587..5888
                     /gene="rstB"
                     /locus_tag="ACHUOY_001822"
     CDS             4587..5888
                     /gene="rstB"
                     /locus_tag="ACHUOY_001822"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR0682616.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            5964..6893
                     /gene="tus"
                     /locus_tag="ACHUOY_001823"
     CDS             5964..6893
                     /gene="tus"
                     /locus_tag="ACHUOY_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR0682617.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(6890..8293)
                     /gene="fumC"
                     /locus_tag="ACHUOY_001824"
     CDS             complement(6890..8293)
                     /gene="fumC"
                     /locus_tag="ACHUOY_001824"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR0682618.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(8436..10082)
                     /gene="fumB"
                     /locus_tag="ACHUOY_001825"
     CDS             complement(8436..10082)
                     /gene="fumB"
                     /locus_tag="ACHUOY_001825"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0682619.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10281..11456
                     /gene="manA"
                     /locus_tag="ACHUOY_001826"
     CDS             10281..11456
                     /gene="manA"
                     /locus_tag="ACHUOY_001826"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR0682620.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            11557..13065
                     /locus_tag="ACHUOY_001827"
     CDS             11557..13065
                     /locus_tag="ACHUOY_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0682621.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(13246..14538)
                     /gene="uidC"
                     /locus_tag="ACHUOY_001828"
     CDS             complement(13246..14538)
                     /gene="uidC"
                     /locus_tag="ACHUOY_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR0682622.1"
                     /translation="MAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRADKPSAG
                     AGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDKSFGFA
                     LGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGYWALTP
                     NIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTSRYSLG
                     ASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETALVSGQL
                     TWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNYRLTPQ
                     FTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKGREKRDG
                     STLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
BASE COUNT         3591 a         3729 c         3535 g         3685 t
ORIGIN      
        1 taaaattggc ttaaggaatg tgatatgaaa aatgacgcag acagttacac cgtttaaatg
       61 caataatcag ccacgtttct cgttaataac aataccagta cctggtttgc gcaaggcgaa
      121 ggattatttt tatgaagctt aagaacaccc tcctggcgtc ggcactgctt tctgctacgg
      181 cattctccgt taacgcagca acagaactga caccggagca agcggcagcg gttaaacctt
      241 ttgaccgtgt agtggttacc ggtcgtttta atgctattgg cgaagcggtg aaagccgttt
      301 ctcgtcgcgc agataaagaa ggtgccgcct ctttttatgt tgtcgacact tctgattttg
      361 gtaacagcgg taactggcgt gtggtcgctg acctctataa agccgatgct gaaaaagcag
      421 aagaaacaag taatcgcgta attaacggtg ttgtcgaact gccgaaagat caggctgttc
      481 tgattgaacc gtttgacacg gtcaccgtcc agggcttcta tcgtagccag ccagaagtca
      541 atgatgccat caccaaagcg gcaaaagcga aaggtgccta ctctttctac atcgttcgtc
      601 aaatcgatgc caaccaaggc ggcaaccagc gtattactgc attcatctat aaaaaagatg
      661 ctaagaaacg tatcgtccag agcccggatg tgatcccggc agattccgaa gcaggacgtg
      721 cagctctggc tgccggtggc gaagccgcga agaaagttga gatcctaggt gttgcgacta
      781 ccgcatcacc aagttctgaa gtcggtcgtt tctttgaaac ccagtcatca aaaggcgggc
      841 gttacaccgt catgctcccg gatggcacta aagtcgaaga actgaacaaa gcgaccgcag
      901 cgatgatggt cccgttcgac agcatcaaat tctctggcaa ctacggcaac atgaccgaag
      961 tctcctatca ggttgcgaaa cgtgccgcga agaaaggtgc taagtattac cacatcaccc
     1021 gccagtggca ggaacgtggt aataacctga ccgtcagcgc agatctgtat aaataactat
     1081 tcagttactc ctataaggcg gcttgatgcc gcctttcgca tttttagtca caaatttccc
     1141 cgcgaccatt gcatgcagac acatctcttc gtaaaatccc gcgcttcagt ggcaaactcc
     1201 atttttatgc gttttgccct aatatttatt cttcttatca cgttttaatc actggatatc
     1261 gatgaaaaag aaactgggac tgagcgcact caccgcgctg gtattaagct caatgctggg
     1321 cgcgggtgtt ttcagtctgc cgcaaaatat ggcggcagtt gccagcccgg cagcactgct
     1381 catcggctgg ggtattactg gcgctggcat tttattgctg gcctttgcca tgctgatcct
     1441 cacgcgcatt cgtcccgaac ttgacggcgg tatctttacc tatgcccgcg aagggtttgg
     1501 cgagctaatc ggcttttgtt ccgcatgggg atactggctg tgcgcagtca tcgccaacgt
     1561 ctcctatctg gtgatcgttt tttccgcgtt aagctttttt acggacacgc cggaattgcg
     1621 cctgttcggc gatggtaata cctggcaatc gatcgtcggt gcatcggctt tattgtggat
     1681 tgttcacttt ttgatcctgc gcggggtgca aaccgctgcc agcattaacc tggtggcgac
     1741 actggcaaaa ttgttgccgc tgggtctgtt tgttgtgctg gcgatgatga tgttcaaact
     1801 ggataccttc aagctcgact tcaccggact tgcacttggc gtacccgttt gggaacaggt
     1861 gaaaaacacc atgctgatca ccctgtgggt tttcattggt gtggaaggtg ccgtggttgt
     1921 ttctgcgcgt gcgcgtaata aacgtgatgt tggcaaagcg acactgctgg cggttctctc
     1981 cgctctgggc gtttacttgc tggtaacgct gctttcgctg ggtgtggtgg cacgtcctga
     2041 actggctgaa attcgtaacc cgtcaatggc cggtctgatg gtggaaatga tgggaccatg
     2101 gggcgaaatc atcatcgctg ccggtttgat cgtttccgtt tgcggcgcgt acttaagctg
     2161 gaccatcatg gcggcagaag ttccgttcct ggctgccact cataaagcat tcccacgcat
     2221 ttttgcgcgc cagaacgcac aagctgcgcc atccgcctca ctgtggttga ccaacatctg
     2281 cgtgcaaatt tgtctggtac tcatctggct gaccggttcc gattacaaca cgttgctgac
     2341 catcgcctca gaaatgattc tggtgcccta tttcctggtc ggcgcgttct tgctgaaaat
     2401 cgcaacccgt ccactgcata aagcggtagg tgtcggtgcc tgcatttatg gcttatggtt
     2461 attgtacgcc tctggtccaa tgcacttgct gttatccgtt gttctgtatg cgccggggct
     2521 gctggttttc ctgtatgcgc gcaaaacgca tacacatgat aatgtactga accgtcagga
     2581 aatggtgctg attggcatgc tgttaattgc ctcagttccg gcgacctgga tgctggtggg
     2641 ataacgttca tcccatcgtt ggatagcaaa ggagataacg atgggtaaag cccagccctt
     2701 gccaatatta attactggcg gaggtcgtcg catcggcctc gccctcgcct ggcatttcat
     2761 taatcaaaag caaccggtga ttgtcagcta tcggacgcac tatccagcca ttgatggact
     2821 gattaaggca ggtgcgcagt gtattcaggc tgatttttcg accaacgacg gtgtgatggc
     2881 gtttgccgat gaagtactaa aaagccccca tggtctgcgt gctattttgc ataacgccag
     2941 tgcgtggatg gcggaaaaac cgggtgcgcc actggccgac gtactggctt gcatgatgca
     3001 gatccacgtt aataccccat acctgctcaa ccatgcgctg gaaagattac tgcgtgggca
     3061 cggacacgcc gccagcgata tcattcactt taccgattat gtggtggagc gcggtagcga
     3121 caagcatatt gcgtatgctg caagcaaagc ggcactggat aatatgaccc gctcgtttgc
     3181 ccgcaagctg gcaccggaag tgaaagtgaa ctctattgct ccatcgctga tcctgtttaa
     3241 tgaacatgat gatgccgaat atcgacaaca ggcgctgaat aaatcactga tgaaaaccgc
     3301 gcctggcgag aaagaagtga tcgacctggt cgattactta cttaccagtt gctttgtcac
     3361 cggacgcagt ttcccacttg atggcggtcg tcatctgcgt taatgcagct ttatccagca
     3421 tataatcaac acccatgcgc ttatccccca acacgaaaca gaaccaacaa aagcggcggg
     3481 caagcgcatc attccggtga aataccacag cgacaccagg taaacaaaat agggaataat
     3541 cgaccacata ctaaaaatga tggttgcgcg taaggcttca atgccgcgtt cgctggcaac
     3601 aatataatgc gcgataagcg caaaggtcgg gaaaagtgga atcagcccgg cgatataata
     3661 atttttcgtt tttgctaaaa caccaatcaa cagcactacc agcgcaccga gcgcggcttt
     3721 gattaccagc cccatttttt taccttaaca cttccataac aagtcatcag tagaatacct
     3781 gatgaaaact tgtttagaaa cgattgatag taagtaaaaa cagcgcggtg tattgtgacg
     3841 tttttatatc taccgtgaat gttatgaaca ctatcgtatt tgtggaagat gatgcggaag
     3901 tcggttcact gattgccgcg tacctggcaa aacatgatat gcaggttacc gtagagccgc
     3961 gcggcgacca ggccgaagaa accattttgc gagaaaatcc ggatttggtg ttactcgaca
     4021 tcatgctacc aggcaaggac ggcatgacca tttgtcgtga tttacgcgca aagtggtctg
     4081 gaccgattgt tcttctaacc tctctcgata gcgatatgaa ccacatcctg gcactggaaa
     4141 tgggtgcctg cgactatatt ctcaaaacga cgccccctgc tgttttgcta gcgcgtttac
     4201 gtttgcattt gcgtcagaat gagcaagcca cactgaccaa aggtcttcag gaaacgtctc
     4261 tgactcccta caaagccctg catttcggca cgttgaccat cgatcccatc aaccgcgtag
     4321 tcaccctggc taacactgaa atctcgctct cgacagctga tttcgaatta ttgtgggaat
     4381 tagctaccca tgccgggcaa atcatggacc gcgatgcatt gctgaaaaat ttacgcggcg
     4441 tcagttatga cggactggat cgtagcgtgg acgtggctat ttcgcggtta agaaaaaaac
     4501 tgctcgataa cgccgcagaa ccttatcgca ttaaaactgt gcgtaacaaa ggctatcttt
     4561 ttgcgcctca tgcatgggaa taagcgatga aaaaactgtt tatccagttt tacctgttat
     4621 tgtttgtctg cttccttgtg atgtctctgc tggttgggct ggtgtacaaa tttaccgccg
     4681 aacgcgcggg caaacagtcg ctggatgatt tgatgaacag ttcactgtat ctgatgcgca
     4741 gcgaattgcg tgagatcccc ccacacgact ggggtaagac gctgaaagag atggatttaa
     4801 atctctcttt cgatctgcgt gtcgagccac tgagtaaata ccatcttgat gatatttcca
     4861 tgcaccgact gcgtggcggc gaaattgtcg ccctggacga tcagtacacg tttttgcagc
     4921 gtatcccgcg cagccactac gtgctggcag ttggtcctgt tccttatctt tattatctcc
     4981 atcagatgcg attgctggat atcgccctga tcacttttat tgctatttcc ctcgcctttc
     5041 cggtgtttat ctggatgcgt ccgcactggc aggatatgtt aaaactggaa gcagcggcgc
     5101 aacgatttgg cgatgggcat ctcaatgaac gtatccactt tgatgagggt tcgagctttg
     5161 aacgacttgg tatcgcattt aaccagatgg cggacaatat caacgccttg attgccagca
     5221 aaaaacagct tattgacggt atcgctcacg aactgcgaac accgttagtg cgcctgcgtt
     5281 atcgactgga gatgagcgat aacctgagcg ccgccgaatc ccaggcgttg aatcgtgata
     5341 tcagtcaact tgaagcttta attgaagagc tgctgactta tgcccgactc gatcgcccac
     5401 aaaacgagct tcatcttagc gaaccagacc tgccgttgtg gctgtcaacg catctggcag
     5461 acattcaggc agtaacgccc gataaaacgg tacggattaa aacgctcgcg caaggccatt
     5521 atgcggcgtt ggatatgcgc ttaatggagc gcgtgctgga taatttgctc aataacgccc
     5581 tgcggtactg ccattcaacg gttgaaacca gcctgctact gtcggggaat agagcgacat
     5641 taattgttga ggatgatggc ccagggattg ccccagaaaa ccgcgaacat atctttgaac
     5701 cttttgttcg cctcgatccc agccgggatc gctcaaccgg cggctgcggg ctggggctgg
     5761 caattgtcca ctctatagca ctggcaatgg gcggtacggt taattgtgac accagcgaac
     5821 tgggtggtgc tcgcttctcg tttagctggc cgttatggca taacatcccg caatttacct
     5881 ctgcctgaca ctacgcgcac gatggtcaag tcaccaccac tgtgctataa aataagtatg
     5941 ttgtaactaa agtggttaat attatggcgc gttacgatct cgtagaccga ctcaacacta
     6001 cctttcgcca gatggaacaa gagctggctg catttgccgc tcatcttgag caacacaagc
     6061 tattggttgc ccgcgtgttc tctttgccgg aggtaaaaaa agaggatgag cataatccgc
     6121 ttaatcgtat tgaggtaaaa caacatctcg gcaacgacgc gcagtcgctg gcgttgcgtc
     6181 atttccgcca tttatttatt caacaacagt ccgaaaatcg cagcagcaag gccgctgtcc
     6241 gtctgcctgg cgtgttgtgt taccaggtcg ataacctttc gcaagcagcg ttggtcagtc
     6301 atattcagca catcaataaa ctcaagacca cgttcgagca tatcgtcacg gttgaatcag
     6361 aactccccac cgcggcacgt tttgaatggg tgcatcgtca tttgccgggg ctgatcaccc
     6421 ttaatgctta ccgcacgctc accgttctgc acgaccccgc cactttacgc tttggttggg
     6481 ctaataaaca tatcattaag aatttgcatc gtgatgaagt cctggcacag ctggaaaaaa
     6541 gcctgaaatc accacgcagt gtcgcaccgt ggacgcgcga ggagtggcaa agaaaactgg
     6601 agcgagagta tcaggatatc gctgccctgc cacagaacgc gaagttaaaa atcaaacgtc
     6661 cggtgaaggt gcagccgatt gcccgcgtct ggtacaaagg agatcaaaaa caagtccaac
     6721 acgcctgccc tacaccgctg attgcactga ttaatcggga taatggcgcg ggcgtgccgg
     6781 acgttggtga gttgttaaat tacgatgccg acaatgtgca gcaccgttat aaaccacagg
     6841 cgcagccgct tcgtttgatc attccacggc tgcacctgta tgttgcggat taacgcccgg
     6901 ctttcatact gccgaccatc tgttctggcc gtacccagct gtcaaactcg gcttcgctaa
     6961 gataccccag cgcaagggcc gcagctttta aggtcagccc ttctttatgc gcttttttgg
     7021 cgatctccgc ggctttgtca taaccaatgt gggtgttaag cgcagtcacc agcatcagcg
     7081 attcattgag taattgattg attcgctcac gattcggttc aatacccact gcgcagtgtt
     7141 tgttaaaact ttccatgcca tctgccagca agcgcaccga ttgcaggaaa ttgtggatca
     7201 ccattggacg gaagacgttc agttcaaagt taccggaagc gccacccatg ttgatcgcca
     7261 cgtcgttccc cattacctga cagcagagca tggttaatgc ttcacactgg gttgggttca
     7321 ctttccccgg catgattgag ctgcccggct cattttccgg gattgagatt tcaccaattc
     7381 cgcagcgcgg gccagaggcc agccagcgga catcattggc gattttcatc agtgacgcag
     7441 ccaacccttt caacgcgccg tgcgcctgaa ccagggcatc acaggtcgca agcgcttcaa
     7501 atttgttcgg cgcggtaaca aacggtgcac aggtaatgac tgccagttca tctgctacgc
     7561 gacgcgcata ctccggatgg gtatttagtc cagtacccac cgctgtaccg ccgagagcca
     7621 gttccgctac gtgaggcagg ctgtattcga tatgtttgag attatgctcg agcatcgcta
     7681 cccagccgga aatctcctgc cccagtgtta gcggcgtggc gtcctgcaag tgggttcgac
     7741 cgattttgac gatatcggca aatgcacgcg atttttcact cagtgtctgt gtcagggttt
     7801 taacctgcgg aatgagttgc ttgcgcagcg ccagcagcgc cgcaacgtgc atcgccgtcg
     7861 gaaagacatc gttggaactt tggcttttgt tcacgtcgtc gttagggtga actttacgtt
     7921 ccatcccgcg cacgccgccg agtaattcac tggcccggtt agccagcact tcgttcatgt
     7981 tcatgttact ttgcgtgccg gagccggtct gccagatagc cagcgggaat tcgtcgtcat
     8041 gctgtcctgc cagtacttca tccgccgcct gccgaatggc gctcgctttc tcttcagaca
     8101 acaagcctaa atcttcatta acttttgccg ctgcgcgctt ggttagcgcc agcgcatgaa
     8161 tcagtgaggt gggcattttc tccgtcgaaa tgcggaaatg ctccagcgag cgttgagttt
     8221 gtgcgcccca cagcttatct gccgggacat caatcgcccc catcgaatct ttttcgctgc
     8281 gtactgtatt catggcctgc tcctcacctg attaattttt tctttctgtt ttgccttcgt
     8341 taagcaactt ttagctcact tattatttac tatttgataa caaatgtttg gtctttcgtg
     8401 ccatgtaaaa aaaccgcccc gaagggcggc tctgtttatt tcacacagcg agtgcactgt
     8461 gtgagttgta tctgctggaa gaagtcattt cctttatcat ccacaaggat aaacgccggg
     8521 aaatcttcca cttcaatttt ccagatggct tccattccca gttccggata ttcaacacat
     8581 tccaggctct taatacttcc ctgcgccaat acagcggccg gaccaccgat actgccaagg
     8641 tagaagccgc cgtgtttttt acaggcatcc gtcacctgct ggctgcggtt gcctttcgcc
     8701 agcatgatca tacttccgcc ctgcgcttgc agttgatcga cataagaatc catccgtccg
     8761 gcggtcgttg ggccaagaga accggaggca taaccttccg gcgttttggc cggacccgcg
     8821 tagtaaatcg gatgatcttt aatgtactgc ggcagccctt caccgttatc catccgctct
     8881 ttcagtttgg cgtgagcaat atcacgaccg acgataatcg tgccgttaag cgataagcgt
     8941 gtagaaacgg gatactgcga caactgtgcg aggatctctt tcatcggacg gttaaggtca
     9001 acgcgcaccg cttcgccttc tcccgcttta cgcagctctt cagggatata tttacctgga
     9061 ttatgttcca gtttttcgat ccagatcccc tgacggttga tcttcgcttt gatattacgg
     9121 tcagcagagc aggaaacacc cataccaacc ggacaggatg cgccgtgacg tggcaggcga
     9181 atcacgcgta tgtcgtgagc gaagtattta ccaccaaact gcgcacccag accgagattt
     9241 tgcgcttcga tcagcaattc tttttccagt tccacatcgc ggaatgcctg accgtgctca
     9301 ttcccttccg ttggcagttc gtcatagtat ttagcggaag ccagtttcac tgttttaagg
     9361 ttagtttctg cagaagttcc accaattacg aacgcaatat gatacggagg acaggccgcc
     9421 gtacccagcg tgcgcatctt ctcaaccagg taatttttca gtttccccgg cgttagtaac
     9481 gctttggttt cctgatagag atacgtcttg tttgccgaac caccaccttt ggcgatacag
     9541 aggaatttgt actcgtcgcc atcaacggca taaagatcga tctgcgctgg cagattggtg
     9601 ccggtattca cctctttata catatccagc ggcgcgtttt gcgagtagcg caaattatct
     9661 tcgatataag tgttatagac accgcgcgcc agcgccgctt catcacagcc accggtccat
     9721 acacgctgcc cttttttacc aacaataatc gcagtgccgg tatcctgaca ggttggcaga
     9781 acacctttcg ccgcgatgtc ggagttgcgc aggaattgca gcgccacata tttatcattt
     9841 tcgctggctt ccgggtcacg cagaatgtcg gccacctgtt gttggtgagc cggacgcagc
     9901 ataaatgacg catcatgaaa cgcctgacgc gccaacagag ttaacgcttc gggtgcgact
     9961 ttcaaaatct cctgcccttc aaattcagat acgctaacgt gttcgctggt tagcaggtaa
    10021 tactcagtat catctttttt gagtggaaaa ggagcctgat aatgaaaggg tttgtttgac
    10081 attgttctct cacttactgc ctggtttggt tatgctctgg gcgggtgttc cgttgccctg
    10141 ttaaaagcga gtaacaatat cctacacact tttttaacaa aaactgagac tagtacgact
    10201 ttttgcggct ccaggttact tcccgtagga ttcttgcttt aatagtggga ttaatttcca
    10261 cattaaaaca gggattgatc atgcaaaaac tcattaactc agtgcaaaac tatgcctggg
    10321 gcagcaaaac ggcgttgact gaactttatg gtatggaaaa tccgtccagc cagccgatgg
    10381 ccgagctgtg gatgggcgca catccgaaaa gcagttcacg agtgcagaat gccgccggag
    10441 atatcgtttc actgcgtgat gtgattgaga gtgataaatc gactctgctc ggagaggccg
    10501 ttgccaaacg ctttggcgaa ctgcctttcc tgttcaaagt attatgcgca gcacagccgc
    10561 tctccattca ggttcatcca aacaaacgca attctgaaat cggttttgcc aaagaaaatg
    10621 ccgcaggtat cccgatggat gccgccgagc gtaactataa agatcctaac cacaagccgg
    10681 agctggtttt tgcgctgacg cctttccttg cgatgaacgc gtttcgtgag ttttccgaga
    10741 ttgtctccct actccagcca gtcgcaggtg cacatccggc aattgctcac tttttacaac
    10801 agcctaatgc cgaacgttta agcgaactgt tcgccagcct gttgaatatg cagggtgaag
    10861 aaaaatcccg cgcgctggcg attttaaaat cggcgctcga cagtcagcag ggtgaaccgt
    10921 ggcaaacgat tcgtttaatt tctgaatttt acccggaaga cagtggcctg ttctccccgc
    10981 tgttactgaa tgtggtgaaa ttgaatcctg gcgaagcgat gttcctgttc gctgaaacac
    11041 cgcacgctta cctgcaaggc gtggcgctgg aagtgatggc gaactccgat aacgtgctgc
    11101 gtgcgggtct gacgcctaag tacattgata ttccggaact ggttgccaat gtgaaattcg
    11161 aagccaaacc ggctaaccag ttgttgaccc agccggtgaa acaaggtgca gaactggact
    11221 tcccgattcc agtggatgat tttgccttct cgctgcatga ccttagtgat aaagaaacca
    11281 ccattagcca gcagagtgcc gccattttgt tctgcgtcga aggcgatgca acgttgtgga
    11341 aaggttctca gcagttacag cttaaaccgg gtgaatcagc gtttattgcc gccaacgaat
    11401 caccggtgac tgtcaaaggc cacggccgtt tagcgcgtgt ttacaacaag ctgtaagagc
    11461 ttactgaaaa aattaacatc tcttgctaag cttattaaag gcttataaca ccttcaggcg
    11521 gccagtccgc ctgatttcat tttatggata atcattatga ataaatcgct ggtagcggta
    11581 ggcgtcattg ttgcgctagg cgtagtctgg acaggcggcg catggtatac aggcaagaag
    11641 attgaaaccc atctcgaaga catggtcgcg caggcgaacg cgcaactcaa actgacagct
    11701 cctgaatcca acctggaagt gagttatcaa aactatcatc gcggcgtatt cagcagccag
    11761 ttgcaactgt tggtgaaacc cattgccggg aaagaaaatc cgtggattaa aagcggtcag
    11821 agcgtcatct tcaacgaatc ggttgatcat ggtcccttcc cgcttgccca gcttaaaaaa
    11881 ctgaacctga tcccgtcgat ggcatcaatt caaaccacgc tggttaataa cgaagtaagc
    11941 aaaccactgt ttgatatggc aaaaggtgaa acgccttttg agattaactc gcgcattggt
    12001 tacagcggtg attccagttc cgatatttcg ctcaagccac tgaattacga gcaaaaggat
    12061 gaaaaagtcg cctttagcgg cggcgagttc cagttaaatg ctgacagaga cggcaaagcc
    12121 atctcccttt ccggggaggc gcaaagtggt cggatagacg cagttaacga atacaaccag
    12181 aaagtgcagt tgacctttaa taatctgaaa accgacggtt ccagcacgct ggcaagtttt
    12241 ggtgagcgcg taggaaacca aaaactgtca ctggaaaaaa tgaccatttc agtggaaggc
    12301 aaagaactgg cactgctgga aggcatggag atcagcggta aatcggatct ggtcaatgac
    12361 ggtaaaacga tcaatagcca actggattac tcgctaaaca gcctgaaggt acagaatcag
    12421 gatctgggca gcggcaagct gactttaaaa gtcggccaga ttgatggtga agcctggcat
    12481 cagtttagcc agcaatataa cgcgcaaact caggcgctgc tggcacagcc agaaattgcc
    12541 aacaatcccg aactttatca ggagaaagtg acggaagcct tctttagcgc cctgccgctg
    12601 atgttgaaag gcgatccggt gattactatc gcgccgctaa gctggaaaaa cagtcagggt
    12661 gaaagtgcgc tgaatctgtc gctgttcctg aaagatccgg caacgactaa agaagcgccg
    12721 caaacgctgg cgcaggaagt agatcgttcg gttaaatctc tggatgcgaa actgaccatt
    12781 ccggtggata tggcaactga gtttatgact caggtagcga agctggaagg ttatcaggaa
    12841 gatcaagcga aaaaactggc gaaacagcaa gttgaaggtg catcagcaat ggggcagatg
    12901 ttccgtctga ccaccttgca ggacaatacc atcaccacca gcctgcaata tactaacggt
    12961 cagataacgt taaacgggca gaaaatgcca ctggaagatt tcgttggtat gtttgcgatg
    13021 ccagctctta acgttccggc tgtaccggca attccgcagc agtaattcac ggccaaaacg
    13081 ccggagaatt ttcacttcgg cgttttcatc tgaaaacaaa ccagagattg cctgatgcgc
    13141 tacgcttatc aggcctacct tttcctgcaa cactttgaat ttatgagttt ttgtaggctg
    13201 gataaggcgt ttacaccgca tccggcataa aaaacgcgca ctttgtcaac aatctgaaac
    13261 gccggaggtt tttctctccg gcgttatttc tgacttcagc ataaagtcat actgaatact
    13321 cattttcaca tcccagtaat tcaggcgatc gcccagcgta ctgccatcgc gcttttcccg
    13381 ccctttatcg gcagcaagaa agaaattcat cccttgtaac ggcccttcgc tgacccgata
    13441 gttcatacca cctaagatac ccgcgccttc aatccgcaca tctcgtttac tggattcata
    13501 gccgcgagtc acaatctgtg caaatgtcag tgtaaattgc ggcgttaaac gatagttgac
    13561 gcccaattgc caggactgca ttccttcacg gttacgatca agacttaatg aaaagggata
    13621 gccaatgtcg gtatccacca cggcaccatt cattgcatgg cgcaaataac caccactgcc
    13681 aaatacgctt ccccacggcg cattccaggt caattgtcca ctaaccaacg ccgtttcatt
    13741 gggctggctg gtattacgac ttaaaccttc gagctgcgcg taaaagccga gcaactcccc
    13801 tttcaacgta taattcgggt cgagcggtat gcttttctcg agaatacttt gtatctgatt
    13861 ggaaacatct ttctgtaccg atgcccccag agaatagcga ctggtatcat catgccaact
    13921 ggcggctaaa aaagaacgtg cgcgatgttt atcgtatttt cccgtctggc tgttatacac
    13981 cccttcatca cgaataccgg ttgttgaatc agtccataaa aatactcgtg aacgccacat
    14041 tccccacaga tcaatatttg gtgttaaagc ccaataacct aatgctcctt cagaaactgt
    14101 cggtaatgta cgttgcgaac tgccagccat taacggaata cgataaggta agctaccata
    14161 actataatcc gtcccgaagc gacctgattt aagcaggcta ttttcactgg gttttatttt
    14221 tactgccccc agggcaaagc caaaactttt atcaccatca agataccacc gggtactcat
    14281 atcagcacga gcacctaatt tataaacata atatgccccg ccttcaacgc caataatatt
    14341 ggaataataa ccactattga aatcgagcaa tcctccctgt acccatgcgt aaatatctct
    14401 tcccgcgcca gcactgggct tatccgccct gcgcaattcg tttttcagtc tgatgcgtag
    14461 tcccgcctca tcgtcagcca gttgcgccgc ataagcagag ataaggccag aggcagccgt
    14521 caggcaaata acggccatgg
//
LOCUS       DAZOYL010000083        14532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZOYL010000083 DAZOYL010000000
VERSION     DAZOYL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.083"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..924
                     /locus_tag="ACHUOY_001829"
     CDS             <1..924
                     /locus_tag="ACHUOY_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0682623.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="ACHUOY_001830"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="ACHUOY_001830"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR0682624.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /gene="lpoA"
                     /locus_tag="ACHUOY_001831"
     CDS             1891..3927
                     /gene="lpoA"
                     /locus_tag="ACHUOY_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR0682625.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="ACHUOY_001832"
     CDS             3885..4280
                     /locus_tag="ACHUOY_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR0682626.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="ACHUOY_001833"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="ACHUOY_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR0682627.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="dolP"
                     /locus_tag="ACHUOY_001834"
     CDS             4900..5475
                     /gene="dolP"
                     /locus_tag="ACHUOY_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR0682628.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="ACHUOY_001835"
     CDS             complement(5589..6629)
                     /locus_tag="ACHUOY_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0682629.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="ACHUOY_001836"
     CDS             complement(6702..7337)
                     /locus_tag="ACHUOY_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR0682630.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="ACHUOY_001837"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="ACHUOY_001837"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR0682631.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="ACHUOY_001838"
     CDS             complement(7963..8406)
                     /locus_tag="ACHUOY_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR0682632.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="ACHUOY_001839"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="ACHUOY_001839"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR0682633.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="ACHUOY_001840"
     CDS             complement(8746..9249)
                     /locus_tag="ACHUOY_001840"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0682634.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="ACHUOY_001841"
     CDS             complement(9243..9767)
                     /locus_tag="ACHUOY_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR0682635.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="ACHUOY_001842"
     CDS             9976..10971
                     /locus_tag="ACHUOY_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR0682636.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="ACHUOY_001843"
     CDS             10980..11858
                     /locus_tag="ACHUOY_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR0682637.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="ACHUOY_001844"
     CDS             12064..13071
                     /locus_tag="ACHUOY_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR0682638.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="ACHUOY_001845"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="ACHUOY_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR0682639.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
BASE COUNT         3580 a         3989 c         3764 g         3199 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg ta
//
LOCUS       DAZOYL010000084        14337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZOYL010000084 DAZOYL010000000
VERSION     DAZOYL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.084"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            59..601
                     /locus_tag="ACHUOY_001846"
     CDS             59..601
                     /locus_tag="ACHUOY_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR0682640.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            701..1903
                     /locus_tag="ACHUOY_001847"
     CDS             701..1903
                     /locus_tag="ACHUOY_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR0682641.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2123..2905
                     /locus_tag="ACHUOY_001848"
     CDS             2123..2905
                     /locus_tag="ACHUOY_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR0682642.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2920..4124
                     /gene="rhmD"
                     /locus_tag="ACHUOY_001849"
                     /pseudo
     CDS             2920..4124
                     /gene="rhmD"
                     /locus_tag="ACHUOY_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            4181..5470
                     /locus_tag="ACHUOY_001850"
     CDS             4181..5470
                     /locus_tag="ACHUOY_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0682643.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5488..6291
                     /gene="yfaU"
                     /locus_tag="ACHUOY_001851"
     CDS             5488..6291
                     /gene="yfaU"
                     /locus_tag="ACHUOY_001851"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR0682644.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6332..7234)
                     /locus_tag="ACHUOY_001852"
     CDS             complement(6332..7234)
                     /locus_tag="ACHUOY_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0682645.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(7427..8617)
                     /gene="glpC"
                     /locus_tag="ACHUOY_001853"
     CDS             complement(7427..8617)
                     /gene="glpC"
                     /locus_tag="ACHUOY_001853"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR0682646.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8614..9873)
                     /gene="glpB"
                     /locus_tag="ACHUOY_001854"
     CDS             complement(8614..9873)
                     /gene="glpB"
                     /locus_tag="ACHUOY_001854"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR0682647.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(9863..11491)
                     /gene="glpA"
                     /locus_tag="ACHUOY_001855"
     CDS             complement(9863..11491)
                     /gene="glpA"
                     /locus_tag="ACHUOY_001855"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR0682648.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11764..13122
                     /gene="glpT"
                     /locus_tag="ACHUOY_001856"
     CDS             11764..13122
                     /gene="glpT"
                     /locus_tag="ACHUOY_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR0682649.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13127..14203
                     /gene="glpQ"
                     /locus_tag="ACHUOY_001857"
     CDS             13127..14203
                     /gene="glpQ"
                     /locus_tag="ACHUOY_001857"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0682650.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14245..>14337)
                     /locus_tag="ACHUOY_001858"
     CDS             complement(14245..>14337)
                     /locus_tag="ACHUOY_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0682651.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
BASE COUNT         3266 a         3539 c         3956 g         3576 t
ORIGIN      
        1 ggcagtacaa tcccgcagct ttgggaattt tgattaattg cttgtgggaa aggggataat
       61 gaaaaaaatt gcgctagcag gtctggccgg aatgctgttg gtttctgcat cggtcaatgc
      121 aatgagcatc agcggtcagg cgggtaaaga atacaccaat attggtgtcg gttttgggac
      181 tgaatcgacg ggcctggcgt taagcggtaa ctggacacat aacgacgacg acggtgacgt
      241 cgctggcgtg gggctggggt tgaatctgcc tctcgggccg ttaatggcga ccgttggcgg
      301 aaaaggcgtt tacaccaacc cgaattacgg cgatgaaggt tatgccgcag cggtaggagg
      361 tggtttgcag tggaaaattg gcaacagctt ccgtttgttt ggcgagtatt actactctcc
      421 ggattcactc tccagcggta ttaaaagtta tgaggaagcg aatgctggcg cgcgttacac
      481 cattatgcat ccagtcagta ttgaggcggg ttatcgctac ctgaatctgt cgggtaaaga
      541 cggtaaccgc gacaacgccg tggctgacgg cccgtatgtt ggggttaacg ccagtttctg
      601 atcccttccg gcgcggcatt ctgtcgcgcc attcagtttc ttactacgcc ttatctccct
      661 ttgcgttata gtgattcgat aacaaaagca ggagataacc atgttaaaag tggaaatgtt
      721 atccaccggg gatgaagtgt tacacgggca aatcgttgac actaacgctg cctggctggc
      781 cgattttttc tttcatcagg ggttgccatt atctcgccgc aatacggtgg gggataacct
      841 tgatgactta gtcaccattc ttcgcgaacg tagtcagcac gccgatgtgc tgatcgttaa
      901 cggcgggctg ggaccgacca gtgatgattt aagtgcactc gccgctgcga cagcaaaagg
      961 tgaaggcctg gtgctgcatg aagcctggct caaagagatg gaacgctatt tccacgaacg
     1021 tggacgagta atggcaccga gcaaccgtaa acaagcggag ctgcctgcca gtgctgaatt
     1081 tatcaataac ccggtaggca ccgcctgtgg ttttgccgtg cggcttaatc gttgcctgat
     1141 gttctttact cccggcgtac cgtcagaatt taaggtgatg gtcgagcacg aaatcctgcc
     1201 gcgcctgcgc gagcgttttt ctttaccaca gtcgccggtt tgtctgcgtt taactacctt
     1261 tggtcgttcg gaaagcgatc tggcacaaag cctggacact ctacaactgc cgccgggcgt
     1321 aacaatgggc tatcgctcct caatgccgat catcgaactg aaactcaccg gaccggcaag
     1381 cgagcaacag gcgatggaaa aactgtggct ggacgttaaa cgtgttgccg gacagagcgt
     1441 gattttcgaa ggcaccgaag gactgcccgc gcagatcagt cgcgaattgc aaaaccgcca
     1501 gttcagcctg acgttgagcg agcaattcac cggtggttta ttggctttgc aactttctcg
     1561 cgcaggtgct ccattgctgg cgtgtgaagt ggttccttca caggaagaaa ccctggcgca
     1621 aactgcgcac tggattacag aacggcgggc caaccatttt gccgggctgg cactggctgt
     1681 ttcgggtttc gagaacgagc atctcaactt tgcgctagcc acgccagacg gcactttcgc
     1741 tctgcgtgtg cgtttcagca ctacacgcta cagcctggct atccgtcagg aagtgtgcgc
     1801 aatgatggca ctgaatatgc tgcgccgttg gttaaacggc caggatatcg ccagtgagca
     1861 tggctggatt gaggttgttg agtccatgac cttatctgtc tgattccgcc actctgtgcc
     1921 ggtttactgc accggcacat tccccctccg gcaaaacttg tccaacctgt tgtactcaat
     1981 aagaaaaaat ccgtgcactg attcacaaat ttgtgcgtaa tttctccagt ccgctggacg
     2041 aggagcggga tttgatgcgc ttcacaattt tactccccct gagcaacgag actgattcca
     2101 gtatctaaga ctggagtcca gtatgttgga atcaagcaaa gttcccgccc tgacacgggc
     2161 tattgatatt ctcaatttga ttgcccgcat tggcccctgt agtgctgcga cgatcattga
     2221 cacattggga atccctaaaa gcacggccta tttgctgctt aatgaactca ggcgtcagcg
     2281 ttttcttagc cttgatcacc aggaaaattt ttgtttgtgg accaggctgg tggagctttc
     2341 tggtcatgcg ttaagcaaga tggatctccg ggaactggct cgaccgcgtt tgacgcaact
     2401 gatggatacc accgggctgt tatgtcacct ggggattatt gataacggca gcgcttatta
     2461 cattttaaaa gtggagtcat cagccactat cagcgtgcgt tcccatgaag gaaaaagcct
     2521 ttcgctttat cgctccggaa taggtaaatg cctgcttgcc tggcaacctg cagcggtaca
     2581 gcaaagtatt atcgaagggc tggtctggga gcaggcgaca cccacaacga ttactcatcc
     2641 acagcaactt cacgaagaac tggcgcgtat tcgccgtcag ggctggagct atgacaacgg
     2701 cgaagattac cccgatgtcc gttgtgtcgc tgcgccggtg tttaacgcca ataacgagct
     2761 taccgctgcg atctcggtgg tcggtacccg cttacaaatc aatgaagaat accgtgatta
     2821 tctggcgggc aaggccattg cctgcgccag ggatatttca cgtctgttgg gatggaaaag
     2881 tccctttgac ttacaggcct cataacaatg gagaacatca tgaccctacc aaaaatcaaa
     2941 caggttcgcg cctggtttac tggcggtgcg acagcagaaa aaggtgctgg cgcggtgatt
     3001 atcacgacca gggggcgaat cactggattg acgatcatat tgccaccccg atgagtaaat
     3061 accgcgatta cgagcagtca cgccagtcat ttggcattaa cgttcttggc acgttgattg
     3121 ttgaagtcga agcagaaaac ggccagaccg gattcgctgt ttcgacagcc ggtgaaatgg
     3181 gctgttttat tgtcgaaaaa catcttaacc gtttcattga gggtaaatgt gtcagtgata
     3241 tcaaactgat ccatgatcaa atgctcaatg ccaccctgta ttactccggc tctggtggcc
     3301 tggtgatgaa cacgatttcc tgtgtcgatc tggctctgtg ggatctgttc ggcaaagtgg
     3361 tcgggcttcc ggtttataaa cttttaggcg gcgcagttcg tgatgagatt cagttctacg
     3421 ccacaggcgc gcgtccggat ctggcaaaag agatgggctt tatcggtggc aaaatgccga
     3481 cgcactgggg gccacatgat ggcgatgcgg ggatccgcaa agatgccgtt atggtcgcgg
     3541 atatgcgtga aaaatgcggt gaggatttct ggttaatgct cgactgctgg atgagtcagg
     3601 acgtgaacta tgccaccaaa ctggcccacg cttgcgcgcc ctataacctg aaatggatcg
     3661 aagagtgcct gccgccacag cagtatgaag gttatcgcga actgaaacgc aacgcgccag
     3721 tcggaatgat ggtcaccagc ggtgagcacc acggcacact gcaatctttt cgtacgcttt
     3781 cagaaaccgg tatcgacatt atgcagccgg atgttggctg gtgcggtggt ttaaccacgc
     3841 tggtggaaat tgccgcaatc gccaaatcca gggggcaact ggtggtgccg cacggttcgt
     3901 ctgtttactc tcaccatgcg gtgatcacct tcaccaatac gccattcagc gaattcctga
     3961 tgaccagccc ggattgttca acgatgcgtc cgcagtttga cccgattctg ctcaatgagc
     4021 cggttccggt gaatggtcgt attcataaat cagtgcttga taaacccggt tttggcgtcg
     4081 aactcaatcg tgactgcaat ttgaaacgcc cctacagcca ctaatcactc cgggcgttgc
     4141 tgtgacaacg gtaacgccca catattctaa ggagcttttt atgagcaccg ctttgcttga
     4201 cgccgtggtg aagaaaaacc gtgtgcgttt aattccgttt atgttggcgc tgtatgtgct
     4261 ggcgtttctc gaccgttcga atatcggttt tgccaaacag acctaccaga ttgataccgg
     4321 gttgagtaat gaagcttatg cgctgggagc aggcattttc tttgtggtat atgcgtttct
     4381 gggtgttccg gcgaatcttt tgatgcgcaa actgggggcc agaacctgga ttggtacgac
     4441 aacactgctg tggggatttc tttcggcagc catggcatgg gccgatactg aagcgaaatt
     4501 tctgatagtt cgcactctgc ttggtgctgc ggaggccgga tttttccctg gtatgattta
     4561 tctcacctcg caatggtttc cgcagcgtaa tcgcgccagc attatggggc tgttctatat
     4621 gggcgcaccg ctggcgttaa cactgggatc accgctttcc ggcgcgctgt tggagatgca
     4681 tggatttatg gggcatcccg gctggttctg gatgtttgtg attgaaggat tgttggcagt
     4741 cggtgctggg gtattcacat tcttttggct tgatgacaca ccggagcagg cacgttttct
     4801 gagtaaacaa gaaaaaacgt tgcttatcaa tcaactggca agtgaagaac aacagaaagt
     4861 gacttctcgg ctgagcgatg cgctgcgtaa tggccgagtc tggcaactag cgattatcta
     4921 cctgaccatt caggtggcgg tttacggatt aattttcttc ctgccgaccc aggttgcggc
     4981 attgctggga acaaaagtgg gctttacagc gtcggtggtc accgccattc cgtgggttgc
     5041 ggccttgttt gggacctggc ttattccgcg ctattccgat aaaaccggcg aacggcgtaa
     5101 tgtcgcagcg ctgacattac tggcggcggg cattggtatt ggtctgtccg ggctgctttc
     5161 tccagtaatg gcgatcgtag cgctgtgtgt tgcagctatc gggtttattg ccgtgcagcc
     5221 ggtgttctgg acgatgccga cacaactgct ttccggtacg gcgctggctg cggggattgg
     5281 ttttgtaaac ctgtttggtg cagtgggcgg gtttattgcc ccgatcctgc gcgtgaaagc
     5341 agaaacgtta tttgccagcg atgcggcagg attactgaca ctggcagcgg tggcggtcat
     5401 cggttcgctg attattttca ctctgcgtgt aaatcgtact gttgcgcaga ccgacgtggc
     5461 acatcattaa ataggttaag gaacacgatg aacgcattat taaccaatcc ctttaaagaa
     5521 cgtttacgca agggcgaagt gcaaattggt ctgtggttaa gctcgactac ttcttatatg
     5581 gcagaaattg ccgctacttc cggttatgac tggttgctga ttgacgggga gcacgcgcca
     5641 aacaccattc aggatcttta tcaccagcta caggcggtag cgccctatgc cagccaaccc
     5701 gtgatccgtc cggtggaagg cagtaaatcg ctgattaaac aagttctgga tattggcgcg
     5761 caaactctat tgatcccgat ggtcgatact gccgatcagg ctcgtcaggt ggcgtctgcc
     5821 acgcgctatc ctccctacgg tgagcgtggt gttggggcca gtgtggcacg ggctgcgcgc
     5881 tggggacgca ttgagaatta catggcgcaa gtcaacgatt cgctttgtct gttagtgcag
     5941 gtggaaagta aaacggcact ggataacctg gacgagatcc tcgacgtcga agggattgat
     6001 ggcgtgttta ttggaccagc ggatctttct gcgtcgttgg gctacccgga taacgccggg
     6061 cacccggaag tgcagcgaat tattgaaacc agcattcggc ggatccgtgc tgcgggtaaa
     6121 gcggctggtt ttctggctgt ggctcctgat atggcgcagc aatgcctggc gtggggagcg
     6181 aactttgtcg ctgttggcgt tgacacgatg ctctacagcg atgccctgga tcaacgactg
     6241 gcgatgttta aatcaggcaa aaatgggcca cgcataaaag gtagttattg atatcaaagg
     6301 cccatgggga tcggctgtgg gcctgtgtta attagtggtt attcgctgcc agatcggctt
     6361 cgcttagctg ggtggccgcg agcacctggt cacgatcaat gccctgttcc agcatgcgca
     6421 aagcaatttt aatggcttgt tcatgcatac ctttctgcca gccaatttgg tgtccttcct
     6481 gccgtagccg ctcagcaata gtcatcaatc tctccttttg tagtggtgaa cgttcggaaa
     6541 tctcctcaat aaaacgggtg aaacgggagg tatcgccgca ttgcagcaga taattaaaca
     6601 gtgtttgtag ctggctgtca ttagtgaagc ctttaaccaa aagcgtggtg atcctgtcga
     6661 ccataccgat taaatcgcgg tcgcgaatat gcttttgaat cagttcaagc agagctatac
     6721 gccgatgttg catgatctca tcgtcaggta cgatggtgat atccaccaac ggaaaagctt
     6781 cggtgtacaa ctgccgggca agttgcggat cgtcaaactc atccagccag ttaagtgagt
     6841 acgggtaggg cgatgtttcg ccatgataaa acagcaatgg caccaccagc ggaactctgt
     6901 catagccttt atccaggtga cgctgcatgg cggcagtggc atagcgcatt agccgaaaag
     6961 ccatattctt ttctgcagag ctttgatgtt caatcacgca gtagatatag ccgtcaccgt
     7021 cgctggtttc cacggaccac aaaacatccg agtagtaagc gcgtaaactt ttttcaataa
     7081 aactagtggg ttccaggcgt aaggtttgca ggttgcaaag cttgcgcagt ggttctggca
     7141 aatgtatttc gagaaaatcc cgtgcggttt cgggtgtgaa cataaaggtt ttaaataccg
     7201 catcatgcgg ggaggaggtt gttgattcgg tcatggttgt ccgtcacctg atatttatga
     7261 tgacgcgaca ttacggttat cccaggcctt tgacatctgc cagtttcagc ctttgcgaga
     7321 cgttttcaga ggaattttca ctgcgttgca acgcatacat tgggcacgga atcgtgtacg
     7381 caaaaatgcc cggtaaccac atgcttaccg ggcatcagaa aggagtttaa gccagcgcct
     7441 gggccagtag cgtaatcgga tgttcgcagc gaagacttgt ggacatctca atctgccatt
     7501 tacaggtttc gcagtcggtg accaccagat ctgcgccgct ttcttctatc tggcggaaca
     7561 gtggtgcgcc gatggcttgt gaggtggggt agttctcttt tttgaaaccg taagtacccg
     7621 caataccgca gcactgggaa tccagcaccg ttaactcaag ccccgggatt ttacgcaaca
     7681 gctccagggt gtagagcgtc cagcccattt tttccatatg gcacggagtg tgataaacca
     7741 ctttcagcgg cagcggtttc agcggtaacg ttttgccttc gtccagcttg cgccacagcc
     7801 agcgggttgc cagttcgata tgatcgcgca agcctttgtt gtcgacattc agcacttccg
     7861 ggtattcgtc gcgcagggca aatgtacagg ttgaggaggt ggcaatcacc ggaatgcctt
     7921 ttactcccac agcttcgcgg atcgactcta cattcgtaat tgcctgtttg cgtgctttat
     7981 cggtaaagcc gttggcgatt agcggtacgc cgcagcattt ttctttgctg agcagttgta
     8041 caccgatacc cattgcgttg agcactttaa ttaaatcttt acctaactgc ggatggttgt
     8101 agttaacgaa gcagccgtga aagaaagcga cctggtcttt atattgtgct tgctgagccg
     8161 ctatgctgcg ataccagcga cggaacgtgc cgaaggagta tttcggtagc gtgcgacgat
     8221 gatcgatttt taacgccgca tcaagcagct gccgcaccgg tttcagcgag gtagcggtgt
     8281 tgacgattgg tgcgaacggc gtggaaacgc tacccatcag gtcggtatga ctcaacacaa
     8341 aattacgcag cgacgggcgc gtggtgtcat attttgcccg cgcgcgctgg ataatatcgc
     8401 caatcttcac atcggacggg caggcgactt cacaacgttt gcagttgatg caatatttca
     8461 gcgcctcgtc atacagtgcg ccatctttca aacgcagacg ctcgccatcc ggcccggctt
     8521 gttttggccc tggataaccg ggattcaccc ggctcaccgg gcaggcggtg gtgcagacgg
     8581 tgcacttaat gcagttttcg aagctggtgt cgttcattgt tggcctcctg cgcgttgggc
     8641 aatctgttga gcggcatgta aagcactgac ggcacaaaca ccgccgccgc atccttgggc
     8701 gatgggatca aatccgccca gcaccgaacc gatggcaaac aggttctcaa tggtttgccc
     8761 tgcctgcgac gggcgtagcg tctcatcagt ggttacaccg aactgctgcc acggttgcgg
     8821 cgcaaaaaaa tcgcccttat accattcacc ccgcgtggcg gtttgtagca catcaaggcc
     8881 gagaatcggc tcgcgaatgc cgttacgttc cgctaccagt ccgccactaa agaaactgcc
     8941 gctggcgaga accgcgaaac gtggacgtag cggaatatcg gcgtgattgc gggtccagat
     9001 ttcattcact acgccatttt tacaggtcac ttttttcact tcatcgcccg gcatccacac
     9061 gccaccctgg cgcacaaact ggcgctgtaa ctggttttgc agacgaatgc ccagcacgga
     9121 aggcggcagc gttggcaaaa gcatcagtga acaaggtagt ttttcattca accaacgcca
     9181 cagtttgtca tcggccaggc cgaagcaggc gggcatcagg atcatttcgc aggtattggc
     9241 gacaggaata agcgcatcaa gtaacagcgg ccagttttct tcattatcaa ggaaacgggc
     9301 gatattcacc gcgcgaaatt cggtggcgtt attgcgcagg acatccagtt ccggcagctc
     9361 tatttctgcg gtttcaaccg aaagatccag ttcgcgcaac gaagccgccg caagatgcgc
     9421 ctggaaatcc atcagcccgc ttatccccac tacacatatc ttcttcgcgg gtagcggcca
     9481 gacgggaact tctggcgaac ttaaccaggt tgcacgcaaa gtgccgagcg gggtaacgcg
     9541 ctgatgggcc agttccacgc tgccctgcaa ttgcgcaccg ctttcagcga tcaatgcctg
     9601 cgcctggcaa gcgagatcga gcacgcgttg tggttcgaga agactgtaag gatgggctgg
     9661 tgcctgctga cgcaaagatt ccagtccact gtgaatgtct gtcaccggtt gaccatcagg
     9721 cagatggctc agcaaatcca gcgaaccgga tgagaaatgc agcgcgcttt gaccacgcgt
     9781 gacaatggca cagcgcaggc cgtgtttttg cagttgcagg ccacagagta atccggcgag
     9841 gccgccgccc ataatgacag tatcaaagcg catctttctg ctccttctcc agaccacaca
     9901 gtccctgata aacccagcgg gtaaattcgc tttcgcgcag tgcatctccc caggcgatgg
     9961 gttgcacgcc tttccagcgt tcgttaagga aggtggaaag ttgctcgata gattgcgcgg
    10021 acgtcgtgac gttaaaacgt tgcagcagtc cggcagcgcg gcaggcgcag agttcgccct
    10081 ggcaggtacc catccccaca cgggtacgac ggcgtaaatc cagcaggcta ttaacgttta
    10141 aattttctac cgcgtactgc acttcacccg ctgttaccgc ttcgcactca cataccaggc
    10201 tacggtgcag acgaccttcg ctcagccagg caggcgtgcg atcgccatga cgataaaccg
    10261 cagaaccgcg cagcggggca ggcagggaga tgactttacg caaggtaact tcagcgggtt
    10321 cctgtgaacc aggaagtgcc agatcggcgg tcgtacaggg gcgcgtgttg cccagtttgc
    10381 ggcacacggc gtcggtagcc cattcagcca tcagccgata ggtcatcagt ttgccaccgg
    10441 tgatggtgat aaatccgtcc agaccatcgc gttcagcatg gtcgagcagc acgatgccac
    10501 ggctgacgtt acgtccgctc gggtcgtcat cgctggcaac cagcgggcgc acgccagaat
    10561 aggcccgcaa aatgcgcgtt ttcgccatca cgggggccag tttttcccct tcacgcagca
    10621 gaatatcaac ctcttctgcc gtcactcgat tatcgtcaat ctcgttgtaa tcaatacgta
    10681 aagaggtggt gccaatcagc gaaatggtat cgccaggcac cagaatatcg gcgtcggaag
    10741 gtttacggca gcggttgatc acatgctggt taatgcggtg atccatgatc agcagcgatc
    10801 ctttcgccgg gaacatgcga atgcgcagat cggcatattc ggcaatgtgt tgcccccaga
    10861 tcccggcggc attaaccacg acaggcgcat gaagggcctg agtttcgccg gtgagatggt
    10921 tacgtacacg aacaccgcac accgtcgcgc cttcacgaat cagtcccgtg acttcatgag
    10981 cggtaaggat aacggcaccg tgttctttgg catccagcat gtttgctgcg gtcagacgaa
    11041 atggatcaac ggtgccatcc ggaactttca ccgcgccaat caatgccggg ttaacggcag
    11101 gttcgataat gcgcgcttgc tgcgggtcta tagcttctgc gctgatccct gcttcttcgc
    11161 aggcgcgaat aaaagtggcc tggaaggaga ggtcatcttc cggcagggta ataaacaggc
    11221 cgttggttgg ttcaacgcag tgacgtgcaa tgcgtttcag gatctggttt tcactaatgc
    11281 attcgcgggc cgattccgca tcggttaccg catagcgcgc accgctgtgt agcaggccgt
    11341 ggttacgccc ggtggcaccg gttgcgatgt cgtggcgctc aaccaaaatc acgcgcagcc
    11401 cgcgcagggc acagtcgcgg gcaatcccgg ctcccgttgc gccgccgcca atgataatca
    11461 cgtcacttga ttgcgagtcg cgagttttca ttgttatccc tctgaagttc gttttttacc
    11521 atttagccat agtaaaaaca tgaattgttt gatttcgcgc atattcgctc atgattcgaa
    11581 agtgaagcgt gatttcatgc gtcattttga acattttgta aatcttattt aataatgtgt
    11641 gcggcaattc acatttaatt tatgaatgtt ttcttaacat cgcggcaact caagaaacgg
    11701 caggttctct cactgaatca ggctgttaat cataaataag accacgggcc acggaggcta
    11761 tcaatgttga gtatttttaa accagcgcca cacaaagcgc gcttacctgc cgcggagatc
    11821 gatccgactt atcgtcgatt gcgctggcaa attttcctgg ggatattctt tggctatgcg
    11881 gcttactatt tggttcgtaa gaactttgcg cttgctatgc cttatctggt tgagcaggga
    11941 ttctcacgcg gtgatttagg ttttgccctt tcggggatct cgattgctta tggattttcg
    12001 aaattcatca tgggttcggt atcggatcgc tcgaatccgc gcgttttcct gcccgcaggt
    12061 ttgattctgg cggcggcagt gatgttgttt atgggctttg tgccatgggc gacgtcgagc
    12121 attgcggtga tgtttgtact gttgttcctc tgcggttggt tccaggggat ggggtggccg
    12181 ccgtgtggtc gtactatggt gcactggtgg tcgcagaaag aacgtggcgg cattgtgtca
    12241 gtgtggaact gtgcgcacaa cgtcggtggt ggtattccgc cgctgctgtt cctgctgggg
    12301 atggcctggt tcaatgactg gcatgcggcg ctctatatgc ctgctttctg cgccattctg
    12361 gtggcattat tcgcctttgc gatgatgcgc gataccccgc aatcctgtgg cttgccgccg
    12421 atcgaagagt acaaaaatga ttatccggac gactataacg aaaaagcgga acaggagctg
    12481 acggcgaagc aaatcttcat gcagtacgta ctgccgaaca aactgctgtg gtatatcgcc
    12541 atcgccaacg tgttcgttta tctgctgcgt tacggcatcc tcgactggtc accgacttat
    12601 ctgaaagagg ttaagcattt cgcgctggat aaatcctcct gggcctactt cctttatgaa
    12661 tatgcaggta ttccgggcac tctgctgtgc ggctggatgt cggataaagt cttccgtggc
    12721 aaccgtgggg caaccggcgt tttctttatg acactggtga ccatcgcgac tatcgtttac
    12781 tggatgaacc cggcaggtaa cccaaccgtc gatatgattt gtatgattgt tatcggcttc
    12841 ctgatctacg gtcctgtgat gctgatcggt ctgcatgcgc tggaactggc accgaaaaaa
    12901 gcggcaggta cggcagcggg ctttaccggg ctgtttggtt acctgggcgg ttcggtggcg
    12961 gcgagcgcga ttgttggcta caccgtggac ttcttcggct gggatggcgg ctttatggta
    13021 atgattggcg gcagcattct ggcggttatc ttgttgattg ttgtgatgat tggcgaaaaa
    13081 cgtcgccatg aacaattact gcaaaaacgc aacggaggct aatggcatga aattgaagct
    13141 gaaaaacctt agcatggcga tcatgatgag cactatagtc atgggaagca gtgcaatggc
    13201 ggcggacagc aacgaaaaaa tagtcatcgc ccatcgcggt gccagtggat atttgccgga
    13261 gcatacgctg ccagcaaaag cgatggcgta tgcgcaggga gcggattatc tggaacagga
    13321 tttggtgatg accaaagacg accatctggt tgttctgcat gaccattatc tcgatcgtgt
    13381 tactgatgtt gccgatcgtt tcccggatcg ggcgcgcaaa gacggtcgtt actacgcgat
    13441 agatttcacg ctggatgaaa ttaagtcgct gaaatttacc gaaggtttcg atattgaaaa
    13501 cggtaaaaaa gtacagactt atccggggcg tttcccaatg ggtaagtccg acttccgggg
    13561 gcacaccttt gaagaagaga ttgaatttgt tcaggggtta aatcactcta ccgggaaaaa
    13621 tatcggtatc tatccagaaa tcaaagcgcc gtggttccat catcaggaag ggaaggatat
    13681 tgcggcaaaa acgctggaag tgctgaagaa atatggttac accggtaagg acgataaagt
    13741 ttatttgcaa tgttttgatg ctgatgagct gaagcgtatt aagaatgagc tggaacctaa
    13801 aatgggcatg gatctcaatc tggtacagct gattgcctat accgactgga atgaaacgca
    13861 gcagaaacag ccggacggaa gctgggttaa ttacagctat gactggatgt ttaagccagg
    13921 tgccatgaag caagtcgcgg aatatgccga cggtatcggg ccggattacc atatgttgat
    13981 tgaggaaaca tcgcagccgg gtaatatcaa actcactggc atggtgcaag atgctcagca
    14041 gaataaactg gtagtgcatc cttataccgt gcggtcggat aaactgcctg aatacactac
    14101 tgatgtgaat cagttatatg atgctctgta taacaaagcg ggtgtaaatg ggctgtttac
    14161 tgatttccct gataaggcag taaaattcct taataaagag taatgctact gcaggatggc
    14221 gttcatgcgc catcctgtct catatcaaat ttttccaatc gccactaatt gttccatgca
    14281 catttgtagt aaaaacagtg gactgccttc taccggcttt tctttagcca acaacga
//
LOCUS       DAZOYL010000085        14309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZOYL010000085 DAZOYL010000000
VERSION     DAZOYL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.085"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            22..564
                     /gene="zapC"
                     /locus_tag="ACHUOY_001859"
     CDS             22..564
                     /gene="zapC"
                     /locus_tag="ACHUOY_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR0682652.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(561..1670)
                     /gene="ycbX"
                     /locus_tag="ACHUOY_001860"
     CDS             complement(561..1670)
                     /gene="ycbX"
                     /locus_tag="ACHUOY_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR0682653.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            1914..4022
                     /gene="rlmKL"
                     /locus_tag="ACHUOY_001861"
     CDS             1914..4022
                     /gene="rlmKL"
                     /locus_tag="ACHUOY_001861"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR0682654.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4034..5941
                     /locus_tag="ACHUOY_001862"
     CDS             4034..5941
                     /locus_tag="ACHUOY_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0682655.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQMADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6071..7324
                     /gene="pqiA"
                     /locus_tag="ACHUOY_001863"
     CDS             6071..7324
                     /gene="pqiA"
                     /locus_tag="ACHUOY_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR0682656.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7329..8969
                     /gene="pqiB"
                     /locus_tag="ACHUOY_001864"
     CDS             7329..8969
                     /gene="pqiB"
                     /locus_tag="ACHUOY_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR0682657.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            8966..9529
                     /gene="pqiC"
                     /locus_tag="ACHUOY_001865"
     CDS             8966..9529
                     /gene="pqiC"
                     /locus_tag="ACHUOY_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR0682658.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            9785..9952
                     /gene="rmf"
                     /locus_tag="ACHUOY_001866"
     CDS             9785..9952
                     /gene="rmf"
                     /locus_tag="ACHUOY_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR0682659.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10022..10540)
                     /gene="fabA"
                     /locus_tag="ACHUOY_001867"
     CDS             complement(10022..10540)
                     /gene="fabA"
                     /locus_tag="ACHUOY_001867"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR0682660.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10609..12369)
                     /locus_tag="ACHUOY_001868"
     CDS             complement(10609..12369)
                     /locus_tag="ACHUOY_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0682661.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12555..13007
                     /gene="matP"
                     /locus_tag="ACHUOY_001869"
     CDS             12555..13007
                     /gene="matP"
                     /locus_tag="ACHUOY_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR0682662.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13083..14123)
                     /gene="ompA"
                     /locus_tag="ACHUOY_001870"
     CDS             complement(13083..14123)
                     /gene="ompA"
                     /locus_tag="ACHUOY_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR0682663.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
BASE COUNT         3555 a         3484 c         3839 g         3431 t
ORIGIN      
        1 tatagggtag gggagaggca gatgcgaatt aaaccagacg ataactggcg ttggtattac
       61 gatgaagagc acgatcgaat gatgctcgat ttagccaatg gtatgctatt tcgctcacgt
      121 tttgcgcgca agatgttgac tccagatgcc ttttctcccg caggcttttg cgttgacgac
      181 gccgcgctct atttctcttt tgaagaaaag tgccgcgatt ttaatttatc taaagaacaa
      241 aaagccgaac tggtgctgaa tgcactggta gcaattcgct atctcaaacc gcaaatgccg
      301 aaaagctggc attttgtttc ccatggtgaa atgtgggtgc ccatgccggg cgatgcggcc
      361 tgcgtttggt tgagcgatac tcacgagcag gtcaatttgc tggtcgttga atctggcgaa
      421 aatgccgcac tatgcctgct ggcacaaccc tgcgttgtta ttgcgggtcg cgcgatgcag
      481 ttgggcgacg ccattaaaat catgaacgac aggctgaaac cgcaggttaa tgttgacagc
      541 ttcagcctcg aacaggcagt ctaacgcgcc aacttaagtg cagtcttcgg tacacagcta
      601 cagcaaagaa tggtgccatc atcgcccatt gctgattttt tcagcggcat gacttcgcct
      661 tctaaaagct gaacacggca acttccacaa atgcccgcgc ggcaagaata agggatacga
      721 attccctgat tttctaattg ctccagcaac acctgttggt tatttccacg aaatgcctgt
      781 ccctgccagt caatatctac atttgcgtcc ggttgttgcg tgatgttggc ggtatcatca
      841 gcggcagctg cgccgtaaat ttttgccgga gccgttgcca gaatttccac ctcatcgcca
      901 acgcgaatca cgccgctatt acgggcaatt aaattctgac caaaatcgac atcgccgtta
      961 tcctgggcag tgcggaaaga ttgcaatgtt tttaatggtt cgcctgccgg atgtttttgc
     1021 cctttttctg ggctgacggt ggtgaaaata cagcggctac aaggtttaac cacatcaaac
     1081 accacatcac caatgcgaat cactttccag cgatcttctt cccaggctga cgcgccggaa
     1141 accaccagat tggggcggaa ttgctccatt tttacactgg ccggacaacg ttgttggaga
     1201 tcacgtaacg aggcttcgtt agcaagaagg taaggatagc catcagcaaa tgacagaggt
     1261 acagtgttgt ggcgtttcac gcgccgggtc atttgtggcc ccacccagcg taattgcact
     1321 tcgcgggaga aaaatccact tagccatttg ttgatcgcgt ctggcgcaat tcgcgcggta
     1381 aaatgtgtgc cccaaacttc ggttggtgcg tcttgtgtgg cgaaatcagc aaaacgaaca
     1441 tatgcgctac tgccatctgg tgcggtgaga tgcaagccat catgcacagg tgaaggagta
     1501 aaccgtacca tctggggaaa ctggcgagcg gtaataaacg taccgtcagg ttccgtgatc
     1561 ataaagatgc gatcgaaggc cagaccactg acatcagcca gagcatgtgt aagaccaatg
     1621 ccgcgcatcg atttaacagg atgaataaaa agccgggtta atgtcgccac ggtgcggtcc
     1681 tcaaatgaaa ataagccctc aactttatga catgacgcgc ttattagcta taatgcgcaa
     1741 caattttctt agcgcctgtc ccattaggct attttatttg tcagtttggc cctgggttgt
     1801 gctcgaaatc ctcacgtact tgtgtacgct ccggtttctc cgcgctgtcc atgtccagac
     1861 tgactgcaac aattacgcct actgcggtag gctcttagag taaaagtgac gatatgaatt
     1921 ctctgtttgc cagtacggcc cgtgggctgg aagagctgtt aaaaactgaa ctggaaaacc
     1981 tgggggccgt tgaatgccag gtggttcagg gtggggtcca tttcaagggc gacacacggc
     2041 ttgtttacca gagcctgatg tggagccgcc tggcctcgcg tattatgttg ccgctgggcg
     2101 agtgtaaggt ttacagcgat ttagacctct atctcggtgt tcaggcgatc aactggacag
     2161 agatgtttaa tcctggcgcg accttcgctg tccacttcag tggtttgaat gacaccatcc
     2221 gcaacagtca gtacggcgcg atgaaagtga aagacgcgat cgtcgatgct ttcacgcgga
     2281 aaaatctgcc gcgtccaaat gttgatcgcg atgcgccgga tatccgcgtt aacgtctggc
     2341 tgcataaaga aaccgccagt atcgctcttg atctgagtgg tgatggttta catctgcgtg
     2401 gctatcgcga tcgtgctggt attgcgccga tcaaagaaac cctggcagcc gctattgtga
     2461 tgcgaaccgg ctggcagcca ggaacaccgc tgctcgatcc gatgtgtggt tccggtacgt
     2521 tgctgattga agcggcgatg ctagcgaccg atcgcgcacc aggcttgcac cgtgggcgtt
     2581 ggggctttag cggctgggtg cagcatgatg aagctatctg gcaggaagtg aaagcggaag
     2641 cgcaaactcg tgcccgtaaa ggcctggctg agtatagctc tcatttttac ggttcggaca
     2701 gcgacgcacg ggtgattcaa cgtgcgcgca ctaacgcccg tcttgcgggg attggtgaac
     2761 tgatcacctt tgaggtgaaa gatgtcgcgc aactgaccaa tccgctgccg aaagggccgt
     2821 acggcacagt gttgagcaac ccgccatacg gggaacgtct ggacagcgaa ccggcgctga
     2881 ttgcgctgca tagcctgctg ggtcggatca tgaaaaacca gtttggtggc tggaatctct
     2941 ctttgtttag tgcctcgccg gatctgctaa gctgcttgca gctgcgtgca gacaaacagt
     3001 acaaggcgaa aaacggcccg ctggactgcg tacagaaaaa ttaccacgtt gccgaaagca
     3061 ctccagacag caaaccggcg atggtagcgg aagactacgc caaccgcctg cgtaagaacc
     3121 tcaaaaaatt cgagaagtgg gctcgccagg aaggaattga atgttaccgc ctgtatgacg
     3181 ccgatctacc ggaatataac gttgccgttg accgttatgc cgactgggtg gtggtgcagg
     3241 agtatgcgcc gccaaaaact attgatgctc acaaagcgcg tcagcgtctg ttcgatatta
     3301 tcgctgcaac catttcggta ctggggattg cgccaaacaa actggtgctg aaaacccgtg
     3361 aacgccagaa gggcaaaaac cagtaccaga aactgggcga gaagggcgag tttcttgaag
     3421 ttaccgaata taacgctcac ttgtgggtga acctgacgga ttatctcgat actggtctgt
     3481 tcctcgatca ccgtatcgcc cgccgtatgc tcggtcagat gagcaaaggc aaagatttcc
     3541 tcaacctgtt ctcttatacc ggtagcgcca ccgtgcatgc gggattaggc ggcgcgcgca
     3601 gcaccaccac cgtggatatg tcgcgtactt atctggagtg ggcagaacgc aacctgcgtc
     3661 tgaatggcct gaccgggcgt gcgcatcgcc tgattcaggc cgattgcctg gcgtggctgc
     3721 gtgaggcaaa tgaacagttt gatctgatct ttatcgatcc gccaaccttc tctaactcaa
     3781 aacgaatgga agatgcgttt gatgttcagc gcgatcatat ggcgctgatg aaagatttga
     3841 aacgtctgct gcgtgcaggt gggacgatca tgttctcgaa caacaaacgt ggcttccgta
     3901 tggatctcga cggcctggca aaactgggac tgaaagcaca agaaattacg caaaaaacgc
     3961 tctcccagga tttcgcccgt aaccgccaga tccacaactg ctggctgatt accgcagcct
     4021 gaaaggaata gtaatgtcat taatcagtat gcatggcgca tggctgtcgt tcagcgacgc
     4081 gccgcttctc gataacgcag aactgcatat cgaagataac gaacgtgttt gtctggtggg
     4141 tcgcaacggc gcgggcaaat cgacgttaat gaaaatcctc aaccgtgaac aagggctgga
     4201 tgacggtcgc attatttacg agcaagattt gattgtagct cgtctgcaac aggagccgcc
     4261 gcgtaacgtt gagggtagcg tttatgattt cgttgccgaa ggcattgaag aacaagcgga
     4321 atatctgaaa cgctatcacg atatttcgcg cctggtgatg aacgacccga gcgagaaaaa
     4381 tctcaacgaa ctggcgaagg ttcaggaaca gttggatcac cacaacctgt ggcagctgga
     4441 aaaccgcatc aacgaagtgc tggcgcaact ggggttagat cctaatgttg cgctgtcgtc
     4501 gctttccggc ggctggttgc gtaaagcggc attaggacgc gcgctggtga gtaatccgcg
     4561 cgtgctgttg cttgatgaac cgacaaacca cctggatatt gaaaccatcg actggctgga
     4621 agggttcctt aaaaccttca atgggacgat tattttcatc tcccacgacc gttcgtttat
     4681 ccgcaatatg gcgacgcgca ttgttgatct cgatcgcggc aagctggtga cctatccagg
     4741 gaattacgac cagtacctgc ttgaaaaaga agaagccctg cgcgtggaag aactacaaaa
     4801 tgccgagttc gatcgcaaac tggcgcagga agaggtgtgg atccgccagg ggatcaaagc
     4861 acgccgtacc cgtaatgaag gccgcgtacg cgccctgaaa gcaatgcgtc gcgagcgtgg
     4921 tgaacgtcgc gaagtgatgg gtaccgcaaa gatgcaggtg gaagaggcca gccgctccgg
     4981 taagatcgtt ttcgaaatgg aagacgtttg ctaccaggtt gacggtaagc aactggtgaa
     5041 agatttttcg gcccaggttc tacgtggcga caaaattgcc ctgattggtc cgaatgggtg
     5101 cggcaaaacc acgctgctga aactgatgct cgatcagctt caagcggaca gcgggcgtat
     5161 tcacgttggc accaaactgg aagtggctta tttcgatcag caccgcgcgg aactggatcc
     5221 cgataaaacg gtgatggata accttgccga aggtaagcaa gaggtgatgg ttaacggcaa
     5281 gccacgccac gtattgggct atttgcagga ctttctgttc catccgaaac gggcgatgac
     5341 gccggtacgt gcgctttctg gcggtgagcg gaaccgcttg ctgctggcgc gtttgttcct
     5401 caaaccaagc aacttattga ttcttgacga accgaccaac gatcttgatg tcgaaacgct
     5461 ggaactgctg gaagaactga tcgacagcta tcagggcacg gtattgctgg ttagccacga
     5521 tcgtcagttt gtcgataaca ccgttacaga atgttggatc ttcgaaggcg gcggtaaaat
     5581 tggtcgttat gtcggcggtt atcatgatgc ccgtggtcag caagagcagt atgtggcgct
     5641 caaacagcct gcggtgaaaa aaaccgaaga agccgccgcg gcaaaagcag aaactgtaaa
     5701 acgcagcagt agcaaactaa gctataaatt gcagcgcgaa ctggagcagc taccgcaatt
     5761 gctcgaagat ctggaggcga agctggaagc cctacagacg cagatggcgg atgcttcctt
     5821 cttcagtcag ccacatgagc agacacaaaa agtgctcgcc gatatggctg ctgcagagca
     5881 ggagctggag caagcctttg aacgctggga gtatcttgaa gcgttaaaaa atggtggctg
     5941 atcgcaaaat agtcgattta aacagagaca gctgacaacg gtaaaattgc ctgatgaact
     6001 gggtttatca ggcatgaaaa ccaacacttt gttagccatc tgtaaaatag cgcatcatta
     6061 aggagtacca atgtgcgaac atcatcatgc cgcgaagcac atcctgtgct cgcagtgtga
     6121 catgctggtg gcgttaccgc gccttgagca tggtcagaaa gcggcatgtc cccggtgtgg
     6181 cacaacgtta accgtggcgt gggatgcccc tcggcagcgt ccgaccgcct atgcgttggc
     6241 tgcactgttc atgctgttgc tgtccaactt gtttcctttt gtgaatatga acgttgcagg
     6301 agttaccagt gaaattacat tactggaaat tcccggcgtg cttttttctg aggactacgc
     6361 cagcctcggc acctttttcc tgttgtttgt gcaactggtt cccgcgtttt gtctgataac
     6421 cattctgtta ctggtgaatc gcgcggaatt accggtccgt ttaaaagagc aactggcacg
     6481 ggtgcttttt caactcaaaa cctggggaat ggcggagatt ttcctcgctg gtgtgctggt
     6541 cagtttcgtt aaactgatgg cttacggcag cattggggta ggcagcagct ttctcccctg
     6601 gtgtttattt tgtgtcctgc aactgcgcgc ttttcagtgc gttgatcgtc gctggttatg
     6661 ggacgacatc gccccgatgc cagaactgcg ccagccgcta aaaccaggcg tcacggggat
     6721 acgtcagggg ctgcgttcgt gctcctgttg tacggcaatc cttcctgctg atgaacccgt
     6781 gtgcccgcgt tgtagtacca aagggtacgt tcggcgtaga aacagccagc agtggacact
     6841 cgcgctgctt gtaacgtcca tcatgctgta tcttccggct aatattttgc ccatcatggt
     6901 gacggattta ttaggctcga agatgccgtc gacgattctc gctggggtca ttctgttatg
     6961 gagcgaaggc tcttatcccg tcgctgcggt gatctttctg gccagtatta tggtgccaac
     7021 gttaaagatg atcgccatcg cgtggctgtg ttgggatgcc aaagggcatg gcaagcgcga
     7081 cagtgaaaga atgcatttga tttatgaagt tgttgagttt gtaggccgct ggtcgatgat
     7141 tgacgttttc gttatcgcgg tgctctcggc gctggtgcgt atgggaggtt taatgagtat
     7201 ttatccggca atgggtgcat taatgtttgc tttagtcgtc ataatgacaa tgttttctgc
     7261 tatgacgttt gacccgcgtt tgtcgtggga tcgtcaacct gaatcagagc atgaggagtc
     7321 ctgacagtat ggaatctaat aatggggaag ccaaaatcca gaaagtgaag aactggtctc
     7381 ccgtgtggat atttcctatc gtcacggcgc tcattggggc ctgggttctt ttttatcatt
     7441 acagccatca gggactggaa gtgaccctga tcaccgcgaa tgcggaagga attgaaggtg
     7501 gcaaaaccac cattaaaagc cgtagcgttg acgtcggcgt ggttgaaagc gccacactgg
     7561 ctgatgattt gacgcacgtt gaaatcaaag tgcggctgaa ttctggtatg gaaaaattgc
     7621 tgcataaaga caccgtcttt tgggtggtga aaccgcagat tggtcgcgaa gggattagcg
     7681 gcctgggaac gctgctgtct ggagtttata tcgaactgca gccaggcgcg aaaggcagca
     7741 aaatggataa atacgatttg ctggactcgc caccgttggc cccgcctgat gcgaaaggta
     7801 tccgtgtgat tctcgatagc aaaaaagccg ggcagctctc gccaggagat ccggtgctgt
     7861 tccgtggcta tcgggtaggt tcggttgaaa ccagcacctt cgatacgcaa aaacgcaata
     7921 tcagttatca actgttcatc aatgcacctt atgaccgact ggtgaccagc aatgttcgct
     7981 tctggaaaga tagtggcatt gcggttgatc tgacgtcagc ggggatgcgt gtggagatgg
     8041 gctcattgac aacgctgctt agtggcggtg ttagctttga tgtgccggaa ggtctggatt
     8101 tagggcagcc agtggcaccg aaaacagctt tcgttttgta tgatgatcag aagagcattc
     8161 aggattcgtt gtacaccgat cacattgatt atctgatgtt ctttaaagat tcggtacgcg
     8221 gtctgcaacc gggagctccg gtagaattcc ggggtattcg cctgggtacc gtaagcaaag
     8281 tgccattctt tgcgccgaat atgcgtcaga catttaacga tgattaccgt attccggtac
     8341 tgattcgtat cgagccagag cggctgaaaa tgcagcttgg cgaaaatgcg gatgttgttg
     8401 agcaccttgg cgaattgttg aaacgtggtt tacgcggatc gctgaaaacc ggaaacctgg
     8461 tcactggtgc actgtatgtt gatcttgatt tctatccaaa tacgcctgca ataaccggta
     8521 ttcgtgaatt taatggttat cagattatcc cgactgttag cggcggcctg gcgcaaatcc
     8581 agcaacgact gatggaagcg ttggataaga tcaacaaact gccattgaat ccgatgattg
     8641 aacaggcaac cagtacgctt tctgaaagtc agcgcacaat gaaaaacctg caaacgacgc
     8701 tggatagcat gaacaagatc ctcgccagcc agtcgatgca gcagttgccg acggatatgc
     8761 agtcaacgtt gcgtgaattg aatcgcagca tgcagggctt ccagcctggc tccgcagcct
     8821 acaacaagat ggtggcggat atgcagcgcc ttgatcaggt gttgcgagaa ctgcaaccgg
     8881 tgctgaaaac gctcaatgag aagagtaacg cgctggtatt tgaagcgaag gacaaaaaag
     8941 atccagagcc gaagagggcg aaacaatgaa aaagtggcta gtgacgattg cagcactgtg
     9001 gctggccgga tgcagctccg gcgaaattaa taaaaactat taccagttac ctgtggtgca
     9061 gagcggtaca caaagtaccg ccagccaggg caatcgtctg ttatgggtag agcaggtcac
     9121 tgttcctgac tatctggcgg ggaatggtgt ggtttatcaa accagtgatg tgaagtatgt
     9181 gattgccaac aacaacttgt gggccagccc gttggatcaa caattgcgca acaccctggt
     9241 tgccaacctg agtacgcaac tgcccggctg ggtggttgcc tcccagcctc tgggaagcgc
     9301 ccaggacacg ctcaatgtta ccgtaacgga gtttaacggt cgctatgatg gcaaggtcat
     9361 tgtcagtggt gagtggctgt tgaaccacca gggacaactg atcaaacgtc cgttccgtct
     9421 ggaaggagtg caaactcagg atggttacga tgagatggtt aaagtactgg ccagtgtctg
     9481 gagtcaggaa gccgcttcta ttgcgcaaga gataaagcgt ctaccttaat tataaagatt
     9541 tgtaaatata accgtctccg gtatgttgcc tgaggcggtt tttttgtttc taacgtgcgg
     9601 aaaaagttgt tcctctttac attttttgta caaccgacat acccgtgtag ctcacaaata
     9661 tgacagtggc gtgaattttg cgcattgacg gcagttatga ttcgcggtat tgcttaactg
     9721 tgattgcaca tttagtaatc actgttttct tttccaccag aaaccagtat gagggaaacg
     9781 aggcatgaag agacaaaaac gagatcgcct ggaacgggca catcaacgtg gttatcaggc
     9841 cggcatcgcc ggacgctcaa aagaaatgtg tccctatcag acgctgaatc aaaggtcaca
     9901 atggctggga ggctggcgag aagccatggc ggacagggta gtaatggcct gattctgtct
     9961 ctttaaaaag aaacctccgc attgcggagg tttcgccttt tgatactctg tctgattata
    10021 atcagaaggc agaagtatcc tggaacagac cgactttcag gtcgctggcg gtatagatca
    10081 aacgaccatc aaccagcact tctccatccg ccaggcccat aatcagacga cggttaacaa
    10141 tgcgtttaaa gtgaatacgg taggtcactt ttttcgctgt cggcagtacc tgaccagtga
    10201 atttcacttc gccaacgccc agtgcgcggc ctttaccttc gccgcccagc cagccgaggt
    10261 agaaccctac cagctgccac attgcgtcca ggcccaggca tcccggcata accggatcgc
    10321 caataaagtg gcatccgaag aaccacagat ccggattgat atccagttct gcttcaacat
    10381 accctttgtc gaagttacca cccgtttcgg tcattttgac cacacggtcc atcatcagca
    10441 tattcggtgc tggcaattgc gggcctttag cgccaaacag ttcaccgcga ccagaggcaa
    10501 gaaggtcttc ttttgtatag gattcgcgtt tatctaccat gttctctgta agccttattt
    10561 tattgaagca cgcaggatag ctaacacgtg tacgctgaac aagtccgatc agttcggaat
    10621 aaaccagttc agccaacgta atggccatgg aaaacggtga cgtccttcct gttgcgatgc
    10681 ttgcgcgata cgttcctgga tggtttgcat cagcgtcgtt tggccttcgc catcccacac
    10741 cagatttaat aataacggca gtgcgtcagt cacatcgtct actgcccaga tggtgaattt
    10801 gccttcttct accgctttca ctagttcact gtgaagactt aaatggcgaa cgttagctgt
    10861 ggggataatg acaccttgtt tcccggttaa ctcacgttgc tggcaaatag caaagaagcc
    10921 ttcgattttc tcatttaaac caccgaccgg ctgggcgcga ccgaactgat cgactgaacc
    10981 tgtgatagcg atactctgat tcaccggcac atcggcgagg gcgcttatca gggcgcagag
    11041 ttcagccatc gaggcactat ctccatcaac ttcactgtat gactgctcaa atgtcagcga
    11101 tgctgagaag gggatctgtt gctcaagctg tagttccgac atcaggaacg cttgcatgat
    11161 catcatccct ttcgcatgga tattgccgcc aagctccgct ttgcgttcga tgtcggtgaa
    11221 ttcaccatcg ccaatatgca caacgcagct aatgcgagaa ggttcgccaa aagcgcgtgg
    11281 atgacccgga aattcaatga ccgaaagggc gttaatttgc ccgatgcgtt cgccttcggt
    11341 ttcaatcagg atttgctcct gaaggatctc atcctgcata cgttcagcga ggaaaccttc
    11401 gcgccattca cgctgctgca gcattaagtt tagctgctcg ccggagaagg tgtcgccatc
    11461 acacagggag gcgacctctt tacactggcg gaggatccac tgcgggctaa gcggaagtgt
    11521 ttcttgttca ccggtgtagc gtgctgcttc gcggataagt accggccagg catccgctcc
    11581 cggtgcaggt aagtgattat gtctggcggt aaatgtcacc cagcgacacc actgggttac
    11641 tgactccgca tcgacaatct gcagagtatc ttcaaattcg ctataaatag cctgctctga
    11701 aagctctggc tccatctcct ggaaatcagc caatgattcg cgttcgccta ccagaatgac
    11761 tttcagcttc aatggcatcg aaggcacaga gacggggaga gggcgcgact catcaaacgc
    11821 aacccagtca aaacgctcgc ggttaacgat atttttcagc cgcatccaca gcagaggttg
    11881 cgccagcagt gtacgcaaag agatgatgag aataccgcca tttgcctgat gcaccagacc
    11941 aggctgcagg gtaatgtcgc cattaaactg gcgcaggcag ccaaagagtt gctccgcttc
    12001 tacccagtcg gcagcgacaa cttgcgttaa agtcgcaaaa ttatcatctg cactcactgc
    12061 gtgacgtaag cggatggagt ggccggaaac ctcatagtga ccgcccacca gttggcctgc
    12121 atcgctttgt agcgtacgcg cggcattggc aataagattc agatactcag actcttccgg
    12181 ggccttcgcc agcataaaag aggaggatgc tcgcgtatgc agcaactgct ccagcgcaaa
    12241 ttgcagccgc ggttgagtat cactgaataa aggatcgttt tcgtcaatca aatgtggctg
    12301 agcaaatatt tcctgatagc tatcggtatc aggaaccagg tcacgccatg caagtttcgt
    12361 aatggtcaaa gttgatgttt tttagtctgt tgtcaaagcc gcgattatac cgtaaccggc
    12421 actacagcac acgtagaaag caccgacaat actccaggca tgggcgttaa agctcacagg
    12481 atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag ttacacggta
    12541 acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt tggaaatgga
    12601 agtatctggt gaagaagcat cgcgaagggg agttaatcac ccgttacata gaagccagtg
    12661 ccgcccagga agccgtcgat gtgttgctct cgctggaaaa tgaacccgtg ctggtaaatg
    12721 gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcaa accattcggg
    12781 caagacgaaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa tcgatcgatc
    12841 tggaatttat tgtctggcaa cgtctggctg gtcttgcgca gcgtcgcggt aaaacgcttt
    12901 ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac gcgaataaaa
    12961 tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg acgaaagtca
    13021 gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta ccagacgaga
    13081 acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat ctctacgcga
    13141 cgatccggag ccaggcagtc gatcagtgca gcacgctgtt tcacgttgtc acaggtgttg
    13201 ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc cgggatacct
    13261 ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc ctggttgtaa
    13321 gcgtcagaac cgatgcggtc ggtgtaaccc agaacaacta cggaaccgtc tttcggatcc
    13381 aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg tttcagggtt
    13441 gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt ctgtacttcc
    13501 ggtgccggag ctggagccgg agcaactact ggagctgctt cgccctgacc gaaacggtag
    13561 gaaaccccca ggctcagcat gccgttgtcc ggacgagtgc cgatggtgtg tgcgtcaccg
    13621 atgttgttgg tccactggta ttccagacgg gtagcgattt caggagtgat cgcgtactca
    13681 acaccgccag cgaagaccgg agaaacgccg gtgtcgtggt ttttaccata aacgttggat
    13741 ttagtgtctg cacgccatac cataccaccc agacgagtgt agatgtccag gtcgtcagtg
    13801 attgggtaac ccagtttagc ggtcagttga acgccctgag ctttgtatgc accgttttca
    13861 acgctgcctt tgtacggcat acgacctaac cagtcgtaac ccatttcaaa gccaacatac
    13921 gggttaacct ggtaaccacc aaaagcacca gcgcccagtt ggttttcatg ggtcgggcca
    13981 ttgttgttga tgaaaccagt gtcatggtac tgggaccagc ccagtttagc accagtgtac
    14041 caggtgttat ctttcggagc ggcctgcgct acggtagcga aaccagccag tgccactgca
    14101 atcgcgatag ctgtcttttt cattttttgc gcctcgttat catccaaaat acgccatgaa
    14161 tatctccaac gagataacac ggttaaatcc ttcaccgggg gatctgctca attaactcta
    14221 ccgatatctt cggcttatgc cgagcacccc tggcgatgta aagtctacaa cgtagttgaa
    14281 aacttacaag tgtgaactcc gtcaggcat
//
LOCUS       DAZOYL010000086        14152 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZOYL010000086 DAZOYL010000000
VERSION     DAZOYL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14152
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.086"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(196..993)
                     /locus_tag="ACHUOY_001871"
     CDS             complement(196..993)
                     /locus_tag="ACHUOY_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0682664.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1003..1554)
                     /gene="ybcL"
                     /locus_tag="ACHUOY_001872"
     CDS             complement(1003..1554)
                     /gene="ybcL"
                     /locus_tag="ACHUOY_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR0682665.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(1723..2055)
                     /gene="emrE"
                     /locus_tag="ACHUOY_001873"
     CDS             complement(1723..2055)
                     /gene="emrE"
                     /locus_tag="ACHUOY_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR0682666.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            2236..2364
                     /locus_tag="ACHUOY_001874"
                     /pseudo
     CDS             2236..2364
                     /locus_tag="ACHUOY_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2389..2703)
                     /gene="fliE"
                     /locus_tag="ACHUOY_001875"
     CDS             complement(2389..2703)
                     /gene="fliE"
                     /locus_tag="ACHUOY_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR0682667.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2919..4577
                     /gene="fliF"
                     /locus_tag="ACHUOY_001876"
     CDS             2919..4577
                     /gene="fliF"
                     /locus_tag="ACHUOY_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR0682668.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4570..5565
                     /gene="fliG"
                     /locus_tag="ACHUOY_001877"
     CDS             4570..5565
                     /gene="fliG"
                     /locus_tag="ACHUOY_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR0682669.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5558..6244
                     /gene="fliH"
                     /locus_tag="ACHUOY_001878"
     CDS             5558..6244
                     /gene="fliH"
                     /locus_tag="ACHUOY_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR0682670.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6244..7617
                     /gene="fliI"
                     /locus_tag="ACHUOY_001879"
     CDS             6244..7617
                     /gene="fliI"
                     /locus_tag="ACHUOY_001879"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR0682671.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7636..8079
                     /gene="fliJ"
                     /locus_tag="ACHUOY_001880"
     CDS             7636..8079
                     /gene="fliJ"
                     /locus_tag="ACHUOY_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR0682672.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8076..9203
                     /locus_tag="ACHUOY_001881"
     CDS             8076..9203
                     /locus_tag="ACHUOY_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR0682673.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9308..9772
                     /gene="fliL"
                     /locus_tag="ACHUOY_001882"
     CDS             9308..9772
                     /gene="fliL"
                     /locus_tag="ACHUOY_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR0682674.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            9777..10781
                     /gene="fliM"
                     /locus_tag="ACHUOY_001883"
     CDS             9777..10781
                     /gene="fliM"
                     /locus_tag="ACHUOY_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR0682675.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10778..11191
                     /gene="fliN"
                     /locus_tag="ACHUOY_001884"
     CDS             10778..11191
                     /gene="fliN"
                     /locus_tag="ACHUOY_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR0682676.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11194..11559
                     /gene="fliO"
                     /locus_tag="ACHUOY_001885"
     CDS             11194..11559
                     /gene="fliO"
                     /locus_tag="ACHUOY_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR0682677.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11559..12296
                     /gene="fliP"
                     /locus_tag="ACHUOY_001886"
     CDS             11559..12296
                     /gene="fliP"
                     /locus_tag="ACHUOY_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR0682678.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12306..12575
                     /gene="fliQ"
                     /locus_tag="ACHUOY_001887"
     CDS             12306..12575
                     /gene="fliQ"
                     /locus_tag="ACHUOY_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR0682679.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12584..13369
                     /gene="fliR"
                     /locus_tag="ACHUOY_001888"
     CDS             12584..13369
                     /gene="fliR"
                     /locus_tag="ACHUOY_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR0682680.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13654..>14152
                     /gene="rcsA"
                     /locus_tag="ACHUOY_001889"
     CDS             13654..>14152
                     /gene="rcsA"
                     /locus_tag="ACHUOY_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR0682681.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKA"
BASE COUNT         3541 a         3586 c         3643 g         3382 t
ORIGIN      
        1 caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg aggaagatag
       61 tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg gattgttcag
      121 gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat gctgttgctc
      181 cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg ttatcttaaa
      241 acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt ccctggcaat
      301 ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta acctcacgtc
      361 gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca atgtggaaac
      421 agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag gttcggatag
      481 cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa gtaaacaatt
      541 aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct gaatttgcgt
      601 tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta actcggaatt
      661 ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat aaagattttg
      721 gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa gacctgcttc
      781 tgggtaattc gcaatataaa attcatatcc acagggaaat aatattatct gattattatc
      841 aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga tacgacatga
      901 agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc gtatttcttc
      961 aatctttagg ttagtcctat cttttgccag catactctta tcctacttta cctcataaac
     1021 gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa ctaacgctcc
     1081 gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt taaactggta
     1141 atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat agccaaaatc
     1201 atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc ctgcatcagc
     1261 gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc atccgctgcc
     1321 tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt ctggagcacc
     1381 agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag aaaatacatg
     1441 agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa atgctgcggc
     1501 ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt tcataaaaac
     1561 ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt atgcaaaaag
     1621 gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat tagccataat
     1681 atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg gtgcgcttcg
     1741 tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta tgatggctgg
     1801 caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa cgataccgac
     1861 tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct gagctaataa
     1921 ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc gtgtaaaccc
     1981 ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc caccaagata
     2041 aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg tactacagtt
     2101 tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc tggcgtggca
     2161 agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa aagagttcac
     2221 tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc gtggatggca
     2281 gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt tgccccatcc
     2341 atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct acacctgcat
     2401 ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc ccatttgcat
     2461 agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc cgggttcacc
     2521 gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta tgcgatcgag
     2581 cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt cctgcgcacg
     2641 cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct gtatcgctga
     2701 cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca ataagacaaa
     2761 ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca
     2821 aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt gttccacttt
     2881 gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact gcagcccaga
     2941 caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg
     3001 ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact
     3061 accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga
     3121 cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa gttccggcag
     3181 ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa ggcggcgcgg
     3241 tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc gaacaggtga
     3301 attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa
     3361 aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat
     3421 ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat gaagggcaaa
     3481 ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg ggaaacgtca
     3541 cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg cgcgatctta
     3601 atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg cgtattgaag
     3661 caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg gcgcaactgg
     3721 acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat gaatctcatg
     3781 cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccgggcg
     3841 gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc
     3901 ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat agcgggccgc
     3961 gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca
     4021 aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct
     4081 tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt gaagatctga
     4141 cccgcgaagc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc gttaactcgc
     4201 cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag caagcgttta
     4261 tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg tggctgctgt
     4321 ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa gctgtacagc
     4381 aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg agcaaagacg
     4441 aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg agccagcgta
     4501 tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc cagtggataa
     4561 ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct gatgaccatt
     4621 ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt gcaaaccctg
     4681 agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta tgtgctggcg
     4741 gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa cgattatctg
     4801 cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct ggaagatatt
     4861 ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga gccgcagagt
     4921 gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct ggtgcatctg
     4981 aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg
     5041 atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga actgaccgaa
     5101 gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga
     5161 acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt tattaccgcc
     5221 gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt cctgttcgaa
     5281 aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa
     5341 tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt cctgcgcaat
     5401 atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt
     5461 ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg ccttgccgaa
     5521 actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc tgccgtggaa
     5581 aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca tggtcgagcc
     5641 ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg cgcaactgca
     5701 aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc agcaaggtca
     5761 tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg cagaggcgaa
     5821 gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat ttcaaactac
     5881 ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc tggaggcggc
     5941 acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca aacagatcca
     6001 acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg tgcacccgga
     6061 tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg gctggcgctt
     6121 gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg aaggcgatct
     6181 cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac caggagtggt
     6241 gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga agccaaaatg
     6301 gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg gctggtgctg
     6361 gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg ccagaacggc
     6421 agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg gctgttttta
     6481 atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc caaaaacatt
     6541 tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt attaggtcgc
     6601 gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac gacggaaacc
     6661 ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga acatgtgctg
     6721 gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca gcgtatgggg
     6781 ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc acgttacacc
     6841 cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt aaaagatttt
     6901 attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc cgctccggcg
     6961 gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat tgccgaagat
     7021 tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg ctacgcgatg
     7081 gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg ttatccaccg
     7141 tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat tagcggcggc
     7201 ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca ggaccccatt
     7261 gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg actggcggaa
     7321 gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat gacggcgttg
     7381 atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc gagttttcag
     7441 cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc gatgctcgat
     7501 aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat ttttgaacgt
     7561 gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt gtcataaccc
     7621 aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga tctggcagaa
     7681 aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg tcagcaggcg
     7741 gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa cctcaacagc
     7801 gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt tatccagacg
     7861 ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca gaaagttgac
     7921 attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca gacactgcag
     7981 gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa aaagatggat
     8041 gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg cccttgatta
     8101 ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct gcacaagatt
     8161 ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa gcggcccccc
     8221 aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc agcgatattg
     8281 tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg cctgtcatca
     8341 acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg ttggctgcgg
     8401 tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa gacctcaccg
     8461 caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc aaagtgactg
     8521 atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa ctgacttctg
     8581 cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag ccattaacac
     8641 cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca ccggtgaccg
     8701 ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc gtcgccgcgc
     8761 ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc cagcatattt
     8821 cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg caggatttag
     8881 gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag atggtttcgc
     8941 cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt acgcagctgg
     9001 ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt agtggtcagc
     9061 agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa cctctggcgg
     9121 gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta acaggcaaca
     9181 gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt cttttccccg
     9241 ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca caggaagacc
     9301 gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt ggctcccgat
     9361 tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact ggcattcgca
     9421 tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg tcttctatgc
     9481 gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata tcggtataac
     9541 cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc cggaagtccg
     9601 tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga cagaagaagg
     9661 caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg ttgccgggca
     9721 accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat aacgacatgg
     9781 gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac agcgaagtca
     9841 aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac gatccgaata
     9901 cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat gagcgctttg
     9961 cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat ataaccgtcg
    10021 gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc
    10081 tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt tcaccgagtc
    10141 tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg accaaagtgg
    10201 aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta aagctggcgc
    10261 ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag tacgtgcgtt
    10321 cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt gtggttaaca
    10381 cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt ctgccattca
    10441 gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg
    10501 aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag ctggagctgg
    10561 tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg
    10621 acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc gtcccggtgc
    10681 tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa catttgatta
    10741 acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga ataatccggc
    10801 cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg aacaaaaatc
    10861 aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag
    10921 cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga ccgtcgagct
    10981 gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt ccgtcgtggc
    11041 gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt taatctccca
    11101 gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata tcattactcc
    11161 gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac tgtgcaatct
    11221 tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat cgccattatt
    11281 gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc gaaacgcact
    11341 ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga acgggttgtg
    11401 gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca aatcaatctg
    11461 ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga ttttcagtcg
    11521 gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg ttgtctgtcg
    11581 cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg ggtatcacca
    11641 gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca
    11701 tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc acccgcatca
    11761 tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg
    11821 tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg atcgacaaaa
    11881 tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag gaggcgctgg
    11941 aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag gcagatttag
    12001 ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc gtgccgatgc
    12061 gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag ataggcttca
    12121 cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg atggcattgg
    12181 ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg ctgtttgtac
    12241 tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac agctagagag
    12301 gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa gtcgcgctgg
    12361 cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc agtattttgc
    12421 aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc atcgccgtat
    12481 ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca
    12541 ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc aggtgacaag
    12601 cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg tgctggcgct
    12661 catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa aactggggct
    12721 ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg ttcctgtttt
    12781 ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg cgcttggttt
    12841 taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat
    12901 ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc ccgttttagc
    12961 gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc atttatggtt
    13021 gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac cgttgaacag
    13081 caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg ggctgatgct
    13141 ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt tactgaatcg
    13201 tatggccccg caattatcca tttttgttat tggatttcca ttaactctga ctgtcggtat
    13261 ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt tattcagtga
    13321 gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat tccgtaacgt
    13381 ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct tccaggatac
    13441 tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta ctttaagaca
    13501 attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa aatcgcgaga
    13561 gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt caggtaaggg
    13621 gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat ggatttatgt
    13681 agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa aaaaagagaa
    13741 atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca gcgcccttca
    13801 gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca gcacatcaag
    13861 cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat tgccaatgtt
    13921 cattttgatg aatatctatt ggtcagaaaa aaattattga taagttctaa atcgattaaa
    13981 ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac gataacctct
    14041 tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt gcgaatgtgg
    14101 atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc ca
//
LOCUS       DAZOYL010000087        14081 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZOYL010000087 DAZOYL010000000
VERSION     DAZOYL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14081
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.087"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUOY_001890"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUOY_001890"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0682682.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHUOY_001891"
     CDS             2273..2662
                     /locus_tag="ACHUOY_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0682683.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUOY_001892"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUOY_001892"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR0682684.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUOY_001893"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUOY_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR0682685.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHUOY_001894"
     CDS             complement(4321..4521)
                     /locus_tag="ACHUOY_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR0682686.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHUOY_001895"
     CDS             4687..5232
                     /locus_tag="ACHUOY_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR0682687.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUOY_001896"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUOY_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUOY_001897"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUOY_001897"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR0682688.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHUOY_001898"
     CDS             complement(6529..7353)
                     /locus_tag="ACHUOY_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR0682689.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUOY_001899"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUOY_001899"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR0682690.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUOY_001900"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUOY_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR0682691.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUOY_001901"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUOY_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR0682692.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUOY_001902"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUOY_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR0682693.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHUOY_001903"
     CDS             complement(11316..11969)
                     /locus_tag="ACHUOY_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR0682694.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUOY_001904"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUOY_001904"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR0682695.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUOY_001905"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUOY_001905"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR0682696.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3430 a         3560 c         3736 g         3355 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta a
//
LOCUS       DAZOYL010000088        14055 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZOYL010000088 DAZOYL010000000
VERSION     DAZOYL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14055
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.088"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(25..1383)
                     /gene="plaP"
                     /locus_tag="ACHUOY_001906"
     CDS             complement(25..1383)
                     /gene="plaP"
                     /locus_tag="ACHUOY_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR0682697.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1373..1435)
                     /gene="yoeI"
                     /locus_tag="ACHUOY_001907"
     CDS             complement(1373..1435)
                     /gene="yoeI"
                     /locus_tag="ACHUOY_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR0682698.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1650..2579)
                     /locus_tag="ACHUOY_001908"
     CDS             complement(1650..2579)
                     /locus_tag="ACHUOY_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0682699.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2625..3449)
                     /locus_tag="ACHUOY_001909"
     CDS             complement(2625..3449)
                     /locus_tag="ACHUOY_001909"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0682700.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3532..3786)
                     /gene="yoeB"
                     /locus_tag="ACHUOY_001910"
     CDS             complement(3532..3786)
                     /gene="yoeB"
                     /locus_tag="ACHUOY_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR0682701.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3783..4034)
                     /gene="yefM"
                     /locus_tag="ACHUOY_001911"
     CDS             complement(3783..4034)
                     /gene="yefM"
                     /locus_tag="ACHUOY_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR0682702.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4317..4367
                     /gene="hisL"
                     /locus_tag="ACHUOY_001912"
     CDS             4317..4367
                     /gene="hisL"
                     /locus_tag="ACHUOY_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR0682703.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4343..4466
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4513..5412
                     /gene="hisG"
                     /locus_tag="ACHUOY_001913"
     CDS             4513..5412
                     /gene="hisG"
                     /locus_tag="ACHUOY_001913"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR0682704.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5418..6722
                     /gene="hisD"
                     /locus_tag="ACHUOY_001914"
     CDS             5418..6722
                     /gene="hisD"
                     /locus_tag="ACHUOY_001914"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR0682705.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6719..7789
                     /gene="hisC"
                     /locus_tag="ACHUOY_001915"
     CDS             6719..7789
                     /gene="hisC"
                     /locus_tag="ACHUOY_001915"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR0682706.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCESGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7789..8856
                     /gene="hisB"
                     /locus_tag="ACHUOY_001916"
     CDS             7789..8856
                     /gene="hisB"
                     /locus_tag="ACHUOY_001916"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR0682707.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8856..9446
                     /gene="hisH"
                     /locus_tag="ACHUOY_001917"
     CDS             8856..9446
                     /gene="hisH"
                     /locus_tag="ACHUOY_001917"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR0682708.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9446..10183
                     /gene="hisA"
                     /locus_tag="ACHUOY_001918"
     CDS             9446..10183
                     /gene="hisA"
                     /locus_tag="ACHUOY_001918"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR0682709.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10165..10941
                     /gene="hisF"
                     /locus_tag="ACHUOY_001919"
     CDS             10165..10941
                     /gene="hisF"
                     /locus_tag="ACHUOY_001919"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR0682710.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10935..11546
                     /gene="hisIE"
                     /locus_tag="ACHUOY_001920"
     CDS             10935..11546
                     /gene="hisIE"
                     /locus_tag="ACHUOY_001920"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR0682711.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11643..12620)
                     /gene="wzzB"
                     /locus_tag="ACHUOY_001921"
     CDS             complement(11643..12620)
                     /gene="wzzB"
                     /locus_tag="ACHUOY_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR0682712.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12766..13932)
                     /gene="ugd"
                     /locus_tag="ACHUOY_001922"
     CDS             complement(12766..13932)
                     /gene="ugd"
                     /locus_tag="ACHUOY_001922"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR0682713.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         3559 a         3640 c         3551 g         3305 t
ORIGIN      
        1 ccggttgatt attagaaaag atgattatgc aacgtcttcg tactgcggaa ctgggttacg
       61 gaagctcttg gtgacgcaag ccaggtaaat cagaccgata gcagcccaaa tcagacccag
      121 aaccattgag ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat
      181 cggcaggaac agatactgga agtgatcttt cagcgtctta ttacgcttct cacggatcca
      241 gaactgcgag atgaccgaca ggttaacgaa ggtgaacgcc accaacgcac caaagttaat
      301 cagcgctgta gccattacca ggtcgaagtt gattgccagc agggcaatcg cgccaaccag
      361 gatgatgttc atcgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg
      421 gaatacgccg tcacgaccca ttacgtacat cagacgcgct acgcctgcat gcgccgccat
      481 accggacgcc agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc
      541 cgcaacgtac agcatgattt caggctgtga cgcatccgga tctttaaagc gagagatatc
      601 cgggaagtac agctgcagga agtaagttgc aaagatgaag atcatgccgc caatcagcgc
      661 ggtcaggaaa atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt
      721 gctgatgccg tcaaagccgg taaaggaaaa gcacaggatt gtcgccccgg taatcatcgg
      781 aataacatgc gcatcgccag accagaacgg acgagtgctc gccagcgtac cagcgccttc
      841 accttcaaat acgccataaa caaccatgcc cagaatcacc gcgatcagca ctacctgcaa
      901 cacgacgatt acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt
      961 cataaaggcc accagcgcca ccacaaacat ccacgatggg atggaaggca ccagagcttc
     1021 aaaatagatt ttcgccagca gaatgttgat catcggcgcg aacagatagt cgagcagaga
     1081 agaccaaccc accataaagc cgacagtcgg gctaatggat ttctgggcgt aagtatatgc
     1141 agagccagca gaaggatagc ggcgaaccag cttcccgtag ctcagagccg taaacaggat
     1201 cgcaatcaat gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat
     1261 accaaaagta tcaaacagcg tcatcggctg catataggca agacccatca ttacaaccgg
     1321 aactaacgta agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga
     1381 catggtcatt ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt
     1441 tggattcctc agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc
     1501 ggcctcttgg tatgaatgat tggtttgaag caaaaaataa ccgacgctga tgaaacgtcg
     1561 gtttttagtc attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag
     1621 aatgagaagc ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt
     1681 gcataaacac cggcgatcgc actatttgct aaaatttcat cccgccacca gccgctggca
     1741 agccctgcgg tctgttcgtt ccagccaatc cagacgggtt caaagagagt ttgggcgacc
     1801 actttcttct cgattaacgc gccactatcg agaaaacgtt gcaccagata acggggtaaa
     1861 tagccgcagc cgaggccgct aatttgcagc tccagcttgg ttttaaaatc gaaaacggta
     1921 atggcttctt gttcgtcgag aagttgtgag gccgtagaag cgcctgcctg cgcagtgtcc
     1981 cccaccacaa tcgcccgata tcgcttaatg atacgacggt ttaacggctc ttcttcctgg
     2041 gctaacggat gatgaggcgc gaccgcaaag acttgttcca gatcgcccaa ccgcgagaaa
     2101 ccaaactcac tggaagatgg tggctcatgc atcgcgccaa caataatatc cgctcgcccc
     2161 tgagtcaggg catcccagga accaccgagt acgccattga taaacttcag gcgcgtcacg
     2221 ctatgatgtt gataaaaagc ttcaatcagt ggcgcaagca gagaaaaagg aaacgtgtcg
     2281 tccacgccaa tcaccagctc gttttcccag ccttcatgca gtttgatcgc ctgcttttcc
     2341 agttctcgca cggtatgcag aacttcccgc cccttctcta ataacatttt tccggtacgg
     2401 gtgaatttag cgcggtgacc gctacgatca agtaattgaa tattaaggtc gctttccagc
     2461 ttgtgaacgg tgtaactcag agcggacggc gtcttgtaga gtttagccga cgccgccgca
     2521 aaactgcctt ctttttctaa ggcatcgagg atcatcaaca catccagcag ttgtttcata
     2581 cacgccccct tgcagtgcat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt
     2641 accagcggat cgggatactg gtattcaaat cccagttcat tacaaatccg actgccatca
     2701 ataatcttac ctttgccgct gtccagacta tttctgaact gcggaggttc cagccccagt
     2761 aaacgggcca tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta
     2821 tagatgtgtc cgcctttagg tgcctgtaac aacagagtga tagcgccaat aacatcttct
     2881 aaatggacta aattaacacc atgttcacca tcaggcgcgg tttttccggc aaagaagcgt
     2941 ccgggatgac gtcccggtcc gaccagcccc gcaagacgca gaatatcgac cgaagtaccg
     3001 ggtaaattgt gcagccagtc ttcgagttct tctaatactc gtccactgtt ggtcaccgga
     3061 ttacgcgggg tggtttcttt caccgtgcct tgcgcgtcgc catagacaga cgttgagctg
     3121 gtaaaaataa tgcgaggaat acgatgggcc agcgcgctat ccactaactc ttgtaccgct
     3181 tgtaaataga actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat gaccagcgca
     3241 tcggcatcca tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga
     3301 agcagatagc tatcaatgcc actcattcgg gccgcttcga caccatcttg tgtggtttta
     3361 ctcccggtga cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac
     3421 cctaacccga caattgcgac ctttttcatc cgttatctcc tgatacacct tagatctata
     3481 aggctacgct agcgtatcaa aactgacaat tcattctatg aatgaatctg ttcaataatg
     3541 ataacgacat gctgcaatga gcagtgaatc gtcggtaacc gcgtatacca gacggtgctc
     3601 ctctgtaatg cgtcgggacc agaaacctga caaattatgt ttcaggggtt ctggcttccc
     3661 cttaccttca aatggcgttc tgcgggtatc tttgataagt tcattgatct ttttaacaat
     3721 tcgcttatct gtttcctgcc agtacagata atcatcccat gattcctcag accagattag
     3781 tttcactcaa taatgtcctt ttctgttcct ttgcctgatt tcaggctatc gattgagtcc
     3841 atcaatctcc gggcgttagc gggggagcgc agtagataag ccgtctcttc cagcgagttg
     3901 tattcttcga gtgacatcag aacacaagcc tctccattct gacgagtaat aaggatcggg
     3961 gcatgatctt caacggcttt catcattgtt gccgacaaat tctgacgcgc ttcgctgtag
     4021 ctaattgtac gcatgtcaat ctcctctttt gtacagttta ttgtacaatg atgggcgtta
     4081 attaactatt tattaattag tttgtagatc aaggtattgt cagtgagacg aaaatccagg
     4141 cttcgctatt tttggtgccg tcagcttaga ggacagtcct cttagccccc tcctttcccc
     4201 gctcattcat taaacaaatc cattgccata aaatatataa aaaagccctt gctttctaac
     4261 gtgaaagtgg tttaggttaa aagacatcag ttgaataaac attcacagag acttttatga
     4321 cacgcgttca atttaaacac caccatcatc accatcatcc tgactagtct ttcaggcgat
     4381 gtgtgctgga agacattcag atcttccagt ggtgcatgaa cgcatgagaa agcccccgga
     4441 agatcacctt ccgggggctt ttttattgcg cggttgataa cggttcagac aggtttaaag
     4501 aggaataaca aaatgacaga caacactcgt ttacgcatag ctatgcagaa atccggccgt
     4561 ttaagtgatg actcacgcga attactggcg cgctgtggca ttaaaattaa tcttcacacc
     4621 cagcgcctga tcgcgatggc agaaaacatg ccgattgata ttctgcgcgt gcgtgacgac
     4681 gacattcccg gtctggtaat ggatggcgtg gtagaccttg ggattatcgg cgaaaacgtg
     4741 ctggaagaag agctgcttaa ccgccgcgcc cagggtgaag atccgcgcta ctttaccttg
     4801 cgtcgtctgg atttcggcgg ctgccgcctt tcgctggcaa cgccggttga tgaagcctgg
     4861 gacggcccgc tctccttaaa cggtaaacgt atcgccacct cttatcctca cctgctcaag
     4921 cgttatctcg accagaaagg catctctttt aaatcctgct tactgaacgg ttctgttgaa
     4981 gtcgccccgc gtgccggact ggcggatgcg atttgcgatc tggtttccac cggtgccacg
     5041 ctggaagcaa acggcctgcg cgaagtcgaa gttatctacc gctcgaaagc ctgccttatc
     5101 cagcgcgatg gtgaaatgga agaatccaaa cagcagctga tcgacaaact gctgacccgt
     5161 attcagggtg tgatccaggc gcgcgaatca aaatacatca tgatgcacgc accgaccgaa
     5221 cgtctggatg aagtcatcgc cctgctgcca ggtgccgaac gcccaaccat tctgccgctg
     5281 gcgggtgacc aacaacgcgt agcgatgcac atggtcagta gcgaaaccct gttctgggaa
     5341 acgatggaaa aactgaaagc gctgggtgcc agttcaattc tggtcctgcc gattgagaag
     5401 atgatggagt gatcgccatg agctttaaca caatcattga ctggaatagc tgtactgcgg
     5461 agcaacaacg ccagctgtta atgcgcccgg cgatctccgc ttctgaaagc attacccgca
     5521 ctgttaacga tattctcgat aacgtgaaag cacgcggtga tgacgccctg cgggaataca
     5581 gcgcgaagtt tgataaaacc acggttaacg cgctgaaggt gtctgctgag gagattgccg
     5641 ccgctagcga acgcctgagc gacgagctaa aacaggcgat ggcggtggca gtaaaaaata
     5701 ttgaaacctt ccacactgcg caaaaactgc cgccggtaga tgtagaaacg cagccaggcg
     5761 tgcgttgcca gcaggtcacg cgcccggtcg cttcagttgg gttgtatatt cctggcggct
     5821 ccgccccgct cttctcaacg gtattaatgc tggcgactcc ggcgcgtatt gcgggctgta
     5881 aaaaagtggt gctgtgctca ccgccgccga ttgccgatga gatcctttat gcggcgcagc
     5941 tgtgcggtgt gaaggacgtg tttaacgtcg gcggcgcaca ggccattgcc gcgctggcgt
     6001 ttggtacgga atctgtgccg aaagtggaca aaatcttcgg gccgggtaac gcctttgtca
     6061 ccgaagcgaa acgtcaggtg agccagcgtc tggacggtgc ggctatcgat atgcccgcag
     6121 gcccgtcgga agtgttggtc attgccgaca gcggcgcaac gccggatttc gtggcttctg
     6181 atttgctttc tcaggctgaa cacggcccgg actcacaggt gattttactg acgcctgacg
     6241 ctgatatggc gcatcaagtt gccgaagccg tcgaacgcca gttagcagaa ctgccgcgtg
     6301 ccgaaaccgc ccgccaggca ctgaacgcca gccgcctgat cgtgactaaa gatttagcgc
     6361 agtgcgtaga gatctccaac cagtacggcc cggagcacct gatcatccag acccgcaacg
     6421 cccgcgatct ggtcgatggc atcaccagcg ccggttcggt atttcttggt gactggtcac
     6481 cggaatcggc aggtgattac gcctccggca ccaaccacgt tctgccgact tacggttaca
     6541 ccgccacctg ttccagcctc gggctggcag atttccagaa gcgtatgacc gtgcaggaac
     6601 tgtcgaaaga aggtttctcc gcgctggctt cgaccattga aacattggcc gccgccgagc
     6661 gcctgaccgc ccataaaaat gccgttactt tgcgtgttaa cgcccttaag gagcaagcat
     6721 gagcaccgtg actattaccg atttagcgcg tgaaaacgtc cgcaacctga cgccgtatca
     6781 gtcggcgcgt cgtctgggcg gtaatggcga cgtctggctg aacgccaacg aataccccac
     6841 tgccgtggag tttcagctta ctcagcaaac gctcaaccgc tacccggaat gtcagccgaa
     6901 agcggtgatt gaaaattacg cgcaatatgc aggcgtaaaa ccggaacagg tgctggtcag
     6961 tcgtggcgcg gacgaaggta ttgaactgct gattcgcgct ttttgcgaat cgggtaaaga
     7021 cgccatcctc tactgcccgc caacgtacgg catgtacagc gtcagcgccg aaacgattgg
     7081 cgtcgagtgc cgcacagtgc cgacgcagga caactggcaa ctggacttac agggcatttc
     7141 cgacaagctg gacggcgtaa aagtggtcta tgtttgcagc cccaacaacc cgacagggca
     7201 actgatcaat ccgcaggatt ttcgcactct gctggagcta acccgcggaa aagccattgt
     7261 ggttgccgat gaagcctata tcgagttttg cccgcaggca tcgctggctg gctggctgac
     7321 ggaatatccg cacctggcta ttttgcgcac actgtcgaaa gcttttgctc tggcgggcct
     7381 tcgttgcgga tttacgctgg caaacgaaga agtcatcaac ctgctgatga aagtgatcgc
     7441 cccctacccg ctctcgacgc cggttgccga cattgcggcc caggcgttaa gcccgcaggg
     7501 aatcgtcgct atgcgcgaac gagtgacgca aattattgca gaacgcgaat acctgattgc
     7561 cgcactgaaa gagatcccct gcgtagagca ggttttcgac tccgcaacca actacattct
     7621 ggcgcgcttt aaagcctcca gcgcagtgtt taaatctttg tgggatcagg gcattatctt
     7681 acgtgatcag aataaacaac cctctttaag cggctgcctg cgaattaccg tcggaacccg
     7741 tgaagaaagc cagcgcgtca ttgacgcctt acgtgcggag caagtttgat gagtcagaag
     7801 tatcttttta tcgatcgcga tggaaccctg attagcgaac cgccgagtga ttttcaggtg
     7861 gaccgttttg acaaactcgc ctttgaacca ggcgtgatcc cggaactgct gaagctgcaa
     7921 aaagcgggct acaaactggt aatgatcacc aatcaggatg gtctgggaac acaaagtttc
     7981 ccgcaggcgg atttcgatgg cccgcacaac ctgatgatgc agatcttcac ctcgcaaggc
     8041 gtacagtttg atgaagtgct gatttgtccg cacctgcccg ccgatgagtg cgactgccgt
     8101 aagccgaaag taaaactggt ggaacgttat ctggctgagc aagcgatgga tcgcgctaac
     8161 agttatgtga ttggcgatcg cgcgaccgac attcaactgg cggaaaacat gggcattaat
     8221 ggtttacgct acgaccgcga aatcctgaac tggccaatga ttggcgagca gctcactaaa
     8281 cgagaccgtt acgctcacgt agtgcgcaac accaaagaga cacagattga cgttcaggtg
     8341 tggctggatc gcgaaggtgg cagcaagatt aacaccggtg ttggcttctt tgatcacatg
     8401 ctggatcaga tcgccaccca cggtggtttc cgtatggaaa tcaacgtcaa aggcgacctc
     8461 tatatcgacg atcaccacac cgtcgaagat accggcctgg cgctgggcga agcgttaaaa
     8521 atggctcttg gcgacaaacg cggtatttgc cgctttggtt ttgtgctacc gatggacgaa
     8581 tgccttgccc gctgcgcgct ggatatctct ggtcgcccgc acctggaata caaagccgag
     8641 tttacctacc agcgcgtggg cgatctcagc accgagatga tcgagcactt cttccgttcg
     8701 ctctcataca ccatgggcgt gacgctacac ctgaaaacca aaggtaaaaa cgatcaccac
     8761 cgtgtagaga gcctgttcaa agcctttggt cgcaccctgc gccaagccat ccgcgtggaa
     8821 ggcgacaccc tgccctcgtc gaaaggagtg ctgtaatgaa cgtggtgatc cttgataccg
     8881 gctgcgccaa tctgaactcg gtgaagtcag ccattgcgcg ccacggttat gaaccaaaag
     8941 tcagccgtga cccggacgtg gtgttgctgg ccgataaact atttttaccc ggcgttggca
     9001 ctgcgcaagc agcgatggat caggtgcgtg agcgcgagct gtttgatctc atcaaagcct
     9061 gtacccaacc ggtactgggc atctgcttag ggatgcaact gctgggtcgg cgcagcgaag
     9121 agagcaacgg cgtcgacttg ctgggcatca ttgacgaaga cgtgccgaaa atgaccgact
     9181 ttggtctgcc gctgccgcat atgggctgga atcgcgttta cccgcaggca ggcaaccgcc
     9241 tgtttcaggg gattgaagac ggcgcgtact tttactttgt tcacagttac gcaatgccgg
     9301 tcaatccgtg gaccattgcc caatgtaatt acggcgaacc gttcaccgcg gcggtacaaa
     9361 aagataactt ctacggcgtg cagttccacc cggagcgttc tggtgccgct ggcgctaagt
     9421 tgctgaaaaa cttcctggag atgtgatgat tattccggca ttagatttaa tcgacggcac
     9481 cgtggtgcgt ctccatcagg gcgattacgg caaacaacgc gattacggta acgacccgct
     9541 gccgcgctta caggattacg ccgcgcaggg tgccgaagtg ctgcatctgg tggatctgac
     9601 cggggcaaaa gatccggcaa aacgtcaaat cccgctgatt aaaaccctgg tcgcgggcgt
     9661 taacgttccg gtgcaggttg gcggcggtgt gcgtagcgaa gaagacgtgg cggcgttact
     9721 ggaagcgggc gttgcgcgcg tggtggttgg ctccaccgcg gtgaaatcac cagaaagggt
     9781 gaaaggctgg tttgaacgtt tcggtgccga tgccttagtg ctggcgctgg atgtccgtat
     9841 tgacgagcaa ggcaacaagc aggtggcggt cagcggctgg caagagaact cgggcgtttc
     9901 actggaacaa ctggtggaaa cctatctgcc tgtcggcctg aaacatgtgc tgtgtaccga
     9961 tatctcgcgc gacggcacgc tggcaggctc taacgtctct ttatatgaag aagtgtgcgc
    10021 cagatacccg caggtggcat ttcagtcctc cggcggtatt ggcgacatta acgatgttgc
    10081 tgccctgcga ggcactggcg tgcgcggcgt aatagttggt cgtgcattac tggaaggtaa
    10141 attcaccgtg aaggaggcca tcgcatgctg gcaaaacgca taatcccatg tctcgacgtt
    10201 cgtgatggtc aggtggtgaa aggcgtacag tttcgcaacc atgaaattat tggcgatatt
    10261 gtaccgctgg caaaacgcta cgccgaagaa ggtgcagacg aactggtgtt ctacgatatc
    10321 accgcttcca gcgatggccg tgtggtagat aaaagctggg tatctcgcgt ggcggaagtg
    10381 atcgacattc cgttctgcgt ggcgggtggg attaagtctc tggaagatgc cgcgaaaatt
    10441 ctttcctttg gcgcggataa aatttccatc aactcccctg cgctggcaga cccaacctta
    10501 attacccgcc tggcggatcg ctttggcgtg caatgcattg tggtcggcat tgatacctgg
    10561 tacgacggcg aaaccggtaa atatcatgtg aatcaatata ccggcgatga aagccgcacc
    10621 cgcgtcactc agtgggaaac actcgactgg gtacaggaag tgcaaaaacg tggagccgga
    10681 gaaatcgtcc tcaacatgat gaatcaggac ggcgtgcgta acggttacga tctcgaacaa
    10741 ctgaaaaaag tgcgtgaagt ttgccacgtc ccgctaattg cctccggtgg cgcgggcacc
    10801 atggaacact tcctcgaagc cttccgcgat gccgacgttg acggcgcgct ggctgcttcc
    10861 gtattccaca aacaaataat caatattggt gaattaaaag cgtacctggc aacacagggc
    10921 gtggagatca ggatatgtta acagaacaac aacgccgcga actggactgg gaaaaaaccg
    10981 acggactaat gccggtaatt gtgcaacacg cggtatccgg cgaagtgtta atgctgggct
    11041 atatgaaccc ggaagcctta gacaaaacca tcgaaagcgg caaagtcacc ttcttctcgc
    11101 gcactaaaca gcgactgtgg accaaaggcg aaacgtcggg caatttcctc aacgtagtaa
    11161 atattgcgcc ggactgcgac aacgacacgc tactggtact ggcgaatcct atcggaccaa
    11221 cctgccacaa aggcaccagc agctgcttcg gcgacaccgc tcaccaatgg ctgttcctgt
    11281 atcaactgga acaactgctc gccgaacgca aatctgccga cccggaaacc tcctataccg
    11341 ccaaactgta tgccagcggc accaaacgca ttgcgcagaa agtgggcgaa gaaggcgtag
    11401 aaaccgcact ggcagcgacg gtacatgacc gctttgagct gaccaacgag gcatcggact
    11461 tgatgtatca cctgctggtg ctgttgcagg atcaggatct ggatttaacg acggtaattg
    11521 agaacctgcg taaacggcat cagtgagttg cagggtaagc ggatgcgatg ttgttgtcgc
    11581 atccggcaaa aaaaaagggc aaggtgtcac caccctgccc gttttcttta aagctgcaaa
    11641 gattacttcg cgttataatt acgcagagcg ttacgcccca gcacaatccc cgcgccaacc
    11701 atgccgccca gcagcaccgc cagaatcaag gtaattgcct ttttcgggct atcgcgacga
    11761 ataggtaacg tcggtttcat cacataacgg taagcatgaa tatcaagatc atcaaccttt
    11821 aagttttcaa tatccaacag gttttgacgt gtctgatagt agtttggtga gaacaccaac
    11881 ggacgggtcg cctcatgctt aatcatcgac tccagcgctt cgctccccaa aaggaacaac
    11941 gtatcttgtg tcacatcttc agtctgctga acctgtggct ttgccacctg cgcctgattc
    12001 gcatactgca acgcttcctg aatctgacgg atacgcagat ctttctgctc ctgcgcgact
    12061 acttcctgcg ttcttaaaga gtcctgcaag tttttccgtc ccagagcaat gttgtctttg
    12121 agatcctttt ctagctcttg attcacttta tcatcaactt gctgaatgta ttgggccaac
    12181 ttcatttgtg ctccctctgc agtttgccca acataagaaa cagtcaatgg taattgctgg
    12241 ttcttaacag aaggttcgat ggtaagtttt tctggttctt cctgattatc cagcgtttct
    12301 gctaatgcag agaaggcaga actgaagcga ccaattaacg tctcctgtaa atccgatact
    12361 ttcggtgcag cctgaccata gataacattc atggcattgt tatagccagc aatttgcccc
    12421 acgtcgggct gagtgataat tgctgttgac gtccatttct ccttcgctac tgccaaatat
    12481 ccaatagcca gggcaatagc cacaatgacg gaaatgatga ttgtcatctt gccacgccac
    12541 aactgcgcta gtaaatcaat caaatcaatc tgttccgggt catggttttg cccagaaaca
    12601 ttattatttt ctactctcat cattacccta actgacggaa atttccaagt ccgcatagtt
    12661 tagcgataat cctagtgaat gctataggat aagtgataaa aataatgaat tattggggag
    12721 gtaacaaaag acaatcaggg cgtaaatagc cctgataaca agatgttagt cgctgccaaa
    12781 gagatcgcgg gtgtatacct tatctgccac atctttaagc tcttctgcca ttcggttaga
    12841 gataatgacg tcggcttgtt gtttgaagat ggcgagatca cgttccagcc gagagttgaa
    12901 gaatgagtct tctttcatca ctggctcgta gatgatcact tcaacgcctt tcgccttgat
    12961 acgcttcata atcccctgaa tagaagacgc acggaagtta tctgaaccgc tcttcataat
    13021 cagacgataa atacccacca cttgcggttt gcgtgacaaa atggcatcgg caataaaatc
    13081 cttacgcgtg cggtttgcat cgacaattgc cgagatcagg ttattcggca cagactgata
    13141 gttcgccagt aactgcttgg tatctttcgg caagcaataa ccaccataac caaacgacgg
    13201 gttgttgtaa tggttgccaa tacgcggatc gagacaaacg ccttcgataa tctggcgagt
    13261 attcagacct aaactttctg cgtagctatc cagttcgtta aagtatgcca cgcgcatcgc
    13321 taggtaggtg tttgcaaaaa gtttaatcgc ttctgcttcg gtcgaatcgg tgaacagggt
    13381 cgggatattt tgcttaatcg cgccttcctg taacaacgca gcaaaacgtt ctgcgcgttc
    13441 tgaacgctca ccgatgacaa tacgtgaagg atggagatta tcgtaaaggg ctttaccttc
    13501 acggagaaat tccggagaga aaataatatt ttcagtacga tatttcttat gcatcgcttc
    13561 ggtaaaacca acgggaaccg ttgatttgat gaccataacc gcataaggat ttatctcaac
    13621 tacgtcttta attactgatt ctacactgga tgtattgaaa taattagttt taggatcata
    13681 gtcggttgga gtggcgatga tgacataatc agcatcccgg taagcttcat ttttatctaa
    13741 tgtggcatta aagtgtattt tatctgattg caaaaactgc tgaatttcct tatcaacaat
    13801 aggagatatc cgatcattca gcatagcaac gcgtgacggt aaaatatcta atgccacaac
    13861 ctcatgattt tgtgcgatta gaagcccgtt tgacaagcct acatagccag taccggaaat
    13921 ggtgattttc atttcatgct ctcagaatta acttaactgt gaatcatgat gtttttagca
    13981 tcctgataag agctaaatgt tttaacgcta cgttattagc tggtcaacgc tacgaaaaac
    14041 aattcagatt ttctt
//
LOCUS       DAZOYL010000089        13807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZOYL010000089 DAZOYL010000000
VERSION     DAZOYL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.089"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHUOY_001923"
     CDS             complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHUOY_001923"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR0682714.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHUOY_001924"
     CDS             1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHUOY_001924"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR0682715.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHUOY_001925"
     CDS             1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHUOY_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR0682716.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHUOY_001926"
     CDS             complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHUOY_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR0682717.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4201..5439
                     /locus_tag="ACHUOY_001927"
                     /pseudo
     CDS             4201..5439
                     /locus_tag="ACHUOY_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHUOY_001928"
     CDS             complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHUOY_001928"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR0682718.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHUOY_001929"
     CDS             complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHUOY_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR0682719.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHUOY_001930"
     CDS             complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHUOY_001930"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR0682720.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHUOY_001931"
     CDS             complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHUOY_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR0682721.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHUOY_001932"
     CDS             complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHUOY_001932"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR0682722.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHUOY_001933"
     CDS             complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHUOY_001933"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR0682723.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHUOY_001934"
                     /pseudo
     CDS             complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHUOY_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHUOY_001935"
     CDS             complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHUOY_001935"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR0682724.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHUOY_001936"
     CDS             complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHUOY_001936"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR0682725.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHUOY_001937"
     CDS             complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHUOY_001937"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0682726.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3390 a         3497 c         3673 g         3247 t
ORIGIN      
        1 atcaataagt tggagtcatt acccccataa tgatattaag cgttttctga tcgggcaacc
       61 ccacggcctg ttgcagcgta ttttccttac tcatgtaata gatagccggc gtgacatttg
      121 cccccagatc gtccatcagt ttctcattgt cacttaacac tttcatttgc tctgtactta
      181 cgtttgcagg cacgtttagc ttaagcttgc caccagaggc ttcatattgt tgccaggttt
      241 ttgcgggatc tttggaggca agaattgccg ctgctgtcgc cgggctttct ggcttgataa
      301 ccccaaccaa caatgttctt aattgcactt tgccagaatc tacccacggg cgcgcctgct
      361 gccagaactg tttacaatat gggcagaacg gatcggcgaa gacgtagaca atgaccggcg
      421 catctttttt accgtcgagg atccagtggg attgttccat ccgttgccac atttcgcgtc
      481 cggctggtgc gtaaatttct ttttcgataa gtgtgttact caggttttca cctttctcgt
      541 tgtacatgta accagagata gcgtgcttac catcgggagt caggtagatg gtgacgccca
      601 tatcctgata ctttccgaga taacctttca ttcctccggg ggcatcgaac gttttgatga
      661 ttgtaatgcc ctgtttttca atcgctttta ctggggaagg aagttcctct gcgaaggcga
      721 ttgcaggaag cagagccagt aaaagtatct tttttaacat taatttgtcc ttttcagtca
      781 gtgcaaaagt cgagtaaaag gcataaccta tcactgtcat aggtaagagc ttagatcagg
      841 tgattgccct ttgtttatga gggtgttgta atccatgtcg ttgttgcatt tgtaagggca
      901 acacctcagc ctgcaggcag gcgctgaaga taccaaaggg tagttcagat tacacggtca
      961 cctggaaagg gggccatttt actttttatc gccgctggcg gtgcaaagtt cacaaagttg
     1021 tcttacgaag gttgtaaggt aaaacttatc gatttgataa tggaaacgca ttagccgaat
     1081 cggcaaaaat tggttacctt acatctcatc gaaaacacgg aggaagtata gatgtccttg
     1141 attaacacca aaattaaacc ttttaaaaac caggcattca aaaacggcga attcatcgaa
     1201 atcaccgaaa aagataccga aggccgctgg agcgtcttct tcttctaccc ggctgacttt
     1261 actttcgtat gcccgaccga actgggtgac gttgctgacc actacgaaga actgcagaaa
     1321 ctgggcgtag acgtatacgc agtatctacc gatactcact tcacccacaa agcatggcac
     1381 agcagctctg aaaccatcgc taaaatcaaa tatgcgatga tcggcgaccc gactggcgcc
     1441 ctgacccgta acttcgacaa catgcgtgaa gatgaaggtc tggctgaccg tgcgaccttc
     1501 gttgttgacc cgcagggtat catccaggca atcgaagtta ccgctgaagg cattggccgt
     1561 gacgcgtctg atctgctgcg taaaatcaaa gcagctcagt acgtagcttc tcacccaggt
     1621 gaagtttgcc cggctaaatg gaaagaaggt gaagcaactc tggctccgtc tctggacctg
     1681 gttggtaaaa tctaaatttc cttcgtcttt cgcgctacca gctccggcgc gaaatacttc
     1741 ggaaattcac ctaattcttc gggtgctgcg gcacccgatt ttcttccccg caccatgatg
     1801 caagctgcat ccaggtagcc gtagaggccg cttgcatgat gatgtttaaa gcccaggaga
     1861 taaacatgct cgacacaaat atgaaaactc aactcaaggc ttaccttgag aaattgacca
     1921 agcctgttga gttaattgcc acgctggatg acagcgctaa atcggcagaa atcaaggaac
     1981 tgttggctga aatcgcagaa ctgtcagaca aagtcacctt taaagaagat aacagcttgc
     2041 cggtgcgtaa gccgtctttc ctgatcacca acccaggttc caaccagggg ccacgttttg
     2101 caggctcccc gctgggccac gagttcacct cgctggtact ggcgttgctg tggaccggtg
     2161 gtcatccgtc gaaagaagcg cagtctctgc tggagcagat tcgccatatt gacggtgatt
     2221 ttgaattcga aacctattac tcgctctctt gccataactg cccggacgtg gtgcaggcgc
     2281 tgaacctgat gagcgtactg aacccgcgca tcaagcacac tgcaattgac ggcggcacct
     2341 tccagaacga aatcaccgat cgcaacgtga tgggcgttcc ggcagtgttc gtaaacggga
     2401 aagagtttgg tcagggccgc atgacgttga ctgaaatcgt tgccaaaatt gatacgggcg
     2461 cggaaaaacg tgcggcagaa gagctgaaca agcgtgatgc ttatgacgta ttaatcgtcg
     2521 gttccggccc ggcgggtgca gcggcagcaa tttactccgc acgtaaaggc atccgtaccg
     2581 gtctgatggg cgaacgtttt ggtggtcaga tcctcgatac cgttgatatc gaaaactaca
     2641 tttctgtacc gaagactgaa gggcagaagc tggcaggcgc actgaaagtt cacgttgatg
     2701 aatacgacgt tgatgtgatc gacagccaga gcgccagcaa actgatccca gcagcagttg
     2761 aaggtggtct gcatcagatt gaaacagctt ctggcgcggt actgaaagca cgcagcatta
     2821 tcgtggcgac cggtgcaaaa tggcgcaaca tgaacgttcc gggcgaagat cagtatcgca
     2881 ccaaaggcgt gacctactgc ccgcactgcg acggcccgct gtttaaaggt aaacgcgtag
     2941 cggttatcgg cggcggtaac tccggcgtgg aagcggcaat tgacctggcg ggtatcgttg
     3001 agcacgtaac gctgctggaa tttgcgccag aaatgaaagc cgaccaggtt ctgcaggaca
     3061 aactgcgcag cctgaaaaac gtcgacatta ttctgaatgc gcaaaccacg gaagtgaaag
     3121 gcgacggcag caaagtcgtt ggtctggaat atcgagatcg tgtcagcggc gatattcaca
     3181 acatcgaact ggccggtatt ttcgtgcaga ttggtctgct gccgaacacc aactggctcg
     3241 aaggcgcagt cgaacgtaac cgcatgggtg agattatcat tgatgcgaaa tgcgaaacca
     3301 acgtcaaagg cgtattcgca gcgggtgact gtacgacggt tccgtacaag cagatcatca
     3361 tcgccactgg cgaaggtgcc aaagcctctt tgagtgcttt tgactacctg attcgcacca
     3421 aaactgcata agaagaagta agattcacct gcaattgctt agccgccggg gtcaaacctg
     3481 gcggcttttt tatggcatta aaaagccctg ccgggatgtt ccaggcaggg cggcataaat
     3541 acttttatct gttagcgaat gaccagtaca ggcagattgg cgtggcggat cacgctcgag
     3601 gcgttagaac ctaacagatg ggtcgaaatc gatgggttgc gagaaccgat aactacaaca
     3661 tcagccccca gttcttctgc caactcattg acttcatccc gcacgctacc aaaacggaca
     3721 tgttgtttaa tgcgggaagg atcgatggtg aagtggctga ccatcgtttg cagacgttct
     3781 tctgcttcat gttgcagatg ctcttcaaaa cgacgcacat cagcggcaaa acggtgcagg
     3841 ctcaggctgg ctgacccggg tagtacgtga agtagatgaa taactccgtc atcctgggcg
     3901 aggaattcag cgtggcgaac agctttgtcg ctcaattcca tttcaaatac atcaactggc
     3961 ataatgattg tcttatacat aaccctttct ccctgttaat catgaacaaa tcattcgcca
     4021 tgattataat atttatccct gatatttgtc tggttctttt tccttacgaa cggtttctgt
     4081 gattaatata ttctcactga acaccaggaa ttctcccaaa acctgcggta ccgcccgttt
     4141 tcccgctgtg atagctaccc ttaaagactg actctttttt gaactgtctc tggaggttgc
     4201 atgaaagcat tgacttatca cggcccacat cacgttcagg tagaaaatgt tcccgatccg
     4261 ggcattgaac aggctgatga tattattctg cgtattacgg caacggcgat ctgtggctct
     4321 gacctccatc tttatcgagg caaaataccc taggttaaac atggcgatat tttgggtcat
     4381 gaatttatgg gggaagtcgt tgaaaccgga aaggacgtaa aaaatttgca aaaaggcgac
     4441 cgggtggtaa ttccgtttgt cattgcttgt ggcgactgtt ttttctgtcg aatgcagcaa
     4501 tatgccgcct gcgaaaatac caatgcgggt aaaggcgctg cgctcaataa aaaacagata
     4561 ccagctcccg cggcattgtt tggttatagt cacctgtatg gtggcgttcc tggtggacag
     4621 gcggaatatg tccgcgtccc taaagggaat gtggggccgt ttaaagtacc gcctttgctt
     4681 tcagatgata aagccctttt cctttctgat attctgccaa cggcatggca ggcagcaaaa
     4741 aatgcgcaga tccaacaagg ttcaagcgtt gcagtctatg gtgccggtcc agtgggattg
     4801 ttgacaatcg cctgtgcacg gttgctcggt gcggaacaga tttttgttgt tgatcatcat
     4861 ccctaccgct tgcgtttcgc cgctgaccgc tacggcgcga tcccgattaa tttcgatgaa
     4921 gatagcgatc cggcacagtc aattattgaa caaacggcag gtcaccgggg cgtggatgca
     4981 gtaatagacg ccgtcggttt tgaagcgaaa ggcagcacca cggaaacggt gctgactaac
     5041 ctgaaactgg agggcagcag cggtaaagcg ttgcgccagt gtattgcggc ggtcaggcgt
     5101 ggcggcattg ttagcgtacc gggcgtctac gctggattta ttcacggttt cctgtttggc
     5161 gacgcctttg ataaagggtt gacgtttaaa atgggacaga cccacgttca cgcatggctg
     5221 ggtgaattat taccgttaat tgagaaagga ttactgaaac cagaagaaat tgttacccac
     5281 tatatgccgt ttgaagaggc cgcccgggga tatgagattt tcgaaaaacg tgaagaggag
     5341 tgccgtaagg tgattctggt acccggtgca caaagcgcag aggcggcgca gaaggcggtt
     5401 tcaggtctgg tgaatgcgat gccgggggga acaatatgat cgtcaggagt ggttttcgag
     5461 gtaaaggaca gccatgacga taatcgccgc cataatcaga aatcctatca ggatgtaaaa
     5521 tgcttctgcc atggttattc ccacaaacga aacggaataa ttttgcagca aaagtgaaca
     5581 gtgagaacca ggaaaaattg ctgattttgc gtaaagagga tgcgagtgca tcctctgggc
     5641 aaagcgagtt atcgcttgtg cagatgggat taaagcaggt agtcgccagc agcttctggc
     5701 tggtattcga gttccagcac ttcaaggtgg gttgcaacgc cgcccggaag ttcccagtga
     5761 atggaatcgc caacgcgcag ccccagcagt gcggcaccta ccggagccat aacggaaagc
     5821 tgagtattgc tatcggtcat ttttgccgga tacaccagcg tgcgcacacg cacttcgcca
     5881 tcgctaagat tgcggaattt aacccggctg ttcattgtca ccacgtcgtg tggcatctct
     5941 tctggcgaac acatttgggc gcgatccaac tctgcgttta acgcgtcggc gattggcaaa
     6001 ccagcatagg cgggttgctc cagcagaata tcgatgcgtt cggcatccag gtcgttaatg
     6061 atgatagttg gtctggacat ttttactcca tgtcgtcggt actgcgagtg tcgcagataa
     6121 acatacccaa aagaaaaccc tcaccgtcag gcggcgaggg tttaactcac atgatgatac
     6181 tgactgttgc tcactctttg aagtgatttg cgtcacattc agggaattcc tcaatgcagc
     6241 gcattatgta taattcttaa tcgtcacggt ttatggaaga cgaattagcg tgttttgtaa
     6301 atcagatgat taataaccca ctttatcaat cacaaagttt ttgccacagt tgcctgggtg
     6361 tggttgtggc aagaatgagt ttgcagaaag cggagcgttg atggcgtcag ctttgatcga
     6421 gatctgaatt tcagtcaaat acgcagggtt accctggcac gttagcttaa ctgctttcac
     6481 gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc tcactgtttt
     6541 accgtaatta tccgcaagga attttccggc ttcgctctct ttaatttctt gattcaggcg
     6601 taccatcgta ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt gtttggcata
     6661 ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc atgacttcac ttagtttagc
     6721 ggccgtttcc agtgataacc cggtttccgg cgatgaacac atccggctgg cgtgcgcttc
     6781 tggtagattc gggattgggc gagtagcgca accgaagcgc atccagcggc gttcatcaac
     6841 accacgggca gcaaccgatt taggcaatcc tggccacaga ccatgtacgg tcagaaaatc
     6901 agctttgttg gtcgtttcgg tttgtaggcg acattcatct cgttcgttac gatttcgatc
     6961 gtgttgactc tggcaaaatc cggtttgcca ggagagggcc aggacatagc gatcaaaatc
     7021 gccatactgt tttgcctgca acgctacggc gttggcagaa gagaagggaa gcagagaaac
     7081 cgcgagcaac gcggcgttac gccagaatgc tttcataatg ggtgtggaac tcatacatac
     7141 actgaatact atctattaaa tcataaaaaa gcccgccagg tgggcttacc tggcgggcgt
     7201 gatgatttat tcagcgtttg gcgaacgtat tagttccaca tggcgagaat cggccagcca
     7261 accaacagca acatagagat gtaaatcacc ccgaagattg cgccaagacg ccagtaatct
     7321 tttgatttca catagccaca gccgtaaata atcaccccag gaccggttgc atacggcgtc
     7381 agacagccca tgataccgat agacagcacc agcaggatac acagttgttc cattggtacg
     7441 cccggaatac ctttaccgac ggccagaata accgacagca tggttgcggt gtgcgcagac
     7501 aggctggcaa acaggtagtg tgcaaagtag aacaccagaa ccagtacaat caccgttgcg
     7561 tttggtgaga atccttccag gtgcgtactc atggtaccgg cgaaccagtc aataaagcca
     7621 gaacgagtca ggccgttagc catcacaacc agagttgcca ggttgaccag cgtgttccat
     7681 gcgctgttat agcgggtaat atctttccaa ggcacaacgt gcagggccag cattagcgaa
     7741 actgccagca gaccaactgc agtagcatta atgacttcac tgccaaaaac ccacaaacct
     7801 aagctgagca atacaaggcc aatcagtgtc cactctctgc gtgtcagcgc acccatggtt
     7861 ttcagttcat cacccgccca ggttgccact tcttcactgt gtgtgatttc cggtttgtac
     7921 agcacgtagg aaagccacgg cgcaatgata agcaagataa ccccaaccgg caggaagcag
     7981 aggaaccact gcaaccagct aatctggata ccggcaattt tgctgacgaa ctccagaccc
     8041 agcacgtttg gtgccgcacc ggtgacaaac atggacgaac tcagactggt actaatgacc
     8101 atcatccaca tcaaatagcc gccaatacga cgcgcggacg gatcgttggg gaatgattta
     8161 aacagcggcg gcaggttttt aatgaccgga aaaaccgtac ccccggtacg cgcggtgttg
     8221 gacggtgtaa acggtgccag cagaatgtcg ataatgacaa tcgcataacc caacgtcagc
     8281 gtgcgtttgc ccatgaattt caccaggaaa agggcaatgc gacgacctaa cccggaaact
     8341 tcatacccta atgcaaaaat aaatgcgcca aataccagcc ataccgtggt gctggaaaaa
     8401 ccagccagac cccatttcag cgcctgtttt tgcgcattaa acgctgggtc agctaattct
     8461 ttggcatcaa agagcaggta attactgcca ataacgcaaa tagtaaccgc aataaaactg
     8521 atcgctgttg ccggaattgg ctcgaggatc atgccgacaa tcattgccac aaacacagcg
     8581 aagtaatgcc atgcctgtgg tggcataccg tcggggacag ggataagaaa catgacaccc
     8641 atcaccacca gtggggccaa tagtttccat atattatctt ttgctaaaga catacgggtt
     8701 ctccgaaaat taatatttcc aaatttatca agtgcttaaa taattaaatc tgtgctaaaa
     8761 accaggtaag gatcagcagg tcggcactgc cgcctggact gagatttcgt tcgatacact
     8821 ccctgtcgaa ctgccggaga taatcgagat cggcgggggt tcgaatgccc cctttttgca
     8881 ataatgtttg cgcctcgcgc tgtagccagc gcaggccccc ctcgccaccg cgcgatgcaa
     8941 cgttggtatc gccgttgatc gccatcagta ggagcaaggt atcgagcaat gccagttcag
     9001 gatctaaccc ctgatccagc agagtgaggt aatgcggcaa ggcgtgattg atcaccagtg
     9061 gataacccgc ttcggcttca ccgcgtgcgc cggtaaggcc aagctgctgg tacaaccgtt
     9121 gacctgccgt cagttgtgaa ttattggtac gcagttctcg atcggtcagg ccacggcaga
     9181 aacttgccgc cgtagaacaa acggttgttg gcgttaccgg ttggttgagt tgaagcaaac
     9241 ggccaattgc cgcacatagt agccctaaag aaaaaatgct gcctttatgc gtgtttacgc
     9301 ccgcagtggc gcggaacata tcaccttcgc aagccatacc aattgggcgt aatccgtgga
     9361 gtaccgcttc tggtgctatt tccgcactac aggcaccaaa ttcaatgaaa cggggtagcc
     9421 agccctgaat cgccagcgcg ctgcggtgga aatcttccag cgccatatct ttatgtgcac
     9481 cgcagttaat ccgatccacg aggcctggtt tcggtgacag attgacttca gtcagcatgg
     9541 cgcgccagcc cagcagggcg tactcatcga tcaatgacgt cgcaagcttt gtggttttag
     9601 ttgacgttgc aggcatcgac atcgttcagc agtgcctcca tgcggttgag taaatcggtc
     9661 agttgatggg tttttccacg cgcgcagacg gctgcgcttt gctcgcacaa caggcagcgg
     9721 cgaggcggca gtgaatagtc gcggcgggag agaatttcgc cttcgggcgt caggacatcg
     9781 atatcccata accgcccgag aggatgacta tgttcaagct caatggtggc gagcttgagg
     9841 tcgcgagccg gggcggcaat gctcaacatg ccctccggcc cgctggcgga aaccagtgca
     9901 gcctgctcct gaatttgcca gccctgtttt gcggctaagg cacgcaaggc tgtcacgcca
     9961 tgattaaaaa ttcggcgtgt gacctcgctg tctttaatcg gcccaggcgc aaccacggta
    10021 aaggagacca gtggaacagg atggcgcttg agccagacgt gttgccgtgc ttgcctttca
    10081 tcccggctga cgagcagctc gggaattgac accgcatggt ggctggcgag ttcaggaagc
    10141 aggtgcatgg cttattcctt cacctgatgc acaacatcga tcaccgagcc atcgcggtaa
    10201 cgcacaacgg caacgacgcg gtcggtgaat tcaatcggct gtggttcacc ggtcagcaga
    10261 cgcgcgcgtt cgcgcagcca ctcaatggaa accactttaa tgcccgcttc ctgcagacgt
    10321 tctgccagtt ccggacgtgc cgggttaact gcgataccgt ggtctgtgac cagaatatcg
    10381 acactggagc ctggggtgat gcaggtcagc acgttatcca ccagagtcgg aatacgaccg
    10441 cgtaccagcg gcgcgacgat gatggaaagc gcagaggcaa tcgcggtatc cagtgaccac
    10501 cggaagcacc acgcagtacg ccgtcagagc cggtcagcac gttaacgttg aactgggtgt
    10561 caatttccag cgcgctcagt accaccacgt cgagacgatc aaccgatgcg cctttcgaac
    10621 cccagttagc gtactggttg gcgctgattt cgatgtgatt ggggttacgg gccagcgatt
    10681 gcgcagcatg gctgtcaaag ctctgcacat ccagcagttt gcggatcaga cctttttcgt
    10741 gcagatcaac catcgtcgcg gtaataccgc cgagggcgaa gtcggcgcga atatcgcggc
    10801 tacgcatttt gtcttccagg aaacgggtta ccgccagcga tgcgccgccg gtgccggttt
    10861 gcatggagaa accttctttg aagtagccag agttgacaat cacatccgca gcgctacggg
    10921 caataagcag ttcgcgcggg ttagtggtca tacgggtcgc gccagcgccg atttttgcag
    10981 catcgccaac gcggtcaact ttgacgatca aatcaacctg atcttgctca atgcttgccg
    11041 gattatgcgg ataaggcagc agttcttcgg taagcatcac aacctgtttt gcgttgtcgg
    11101 catcaaccat tgcatagccg agggagccgc agcaggcttt accggtgtaa ccgttggcat
    11161 taccgaattc atcacaggac gggacgccga ggaaagccac gtcgatattc agttcgccgc
    11221 tctgtaccag atgcacacgt ccgccgtgag agtggatttg caccggttcc gcaagcagac
    11281 cacgggaaat ttcttctgcc agtggaccgc gcaggccgga ggtataaatg cgggtaacca
    11341 cgccctggcg aatgtgttct accagtggcg catggcaatc actcagggag ctggacgcca
    11401 gggtcaggtt tttaaagccc atcttagcga tgacgtccat caccatattg acggtcaggt
    11461 caccgccacg gaaagcgtga tggaaggaaa ccgtcatgcc gtcctgtaaa ccagagcgac
    11521 gaatcgcttc ttcctggttg gcgcacagtt tgcgatcgcg cgctttttca gcctggtagg
    11581 tttgctttgg tgagttctgg aaagcggcaa gatcgcattc agcgcgacga ttccaggccg
    11641 ctacccgttc ttgtcgttga gattgttcaa ttttctgcgt cattttgatt gccttattct
    11701 tcgcggatgc cggaaagttc tgcacgggag agcaccagac gggcacgatc gataaccgga
    11761 ccgtccacca tcttgccgtt cagggaaacc acgccgaggc cttcgcgagc ggcggcttca
    11821 gcggcttcta cgacgcggcg ggcgtgatcc acttctttct gggtcggtgc gtagaggttg
    11881 tgcaacagat caatctgacg cgggttgatc agcgatttgc cgtcaaagcc cagctgtttg
    11941 atgtgggcgg cttcttgcag gaatccggct tcgttgttag cgtcggaata gacggtatcg
    12001 aacgcctgaa tacccgcaga gcgcgcggcc tgcaaaatgg aacagcgtgc gaacagcagt
    12061 tcagttcctt ccggggagcg ttctgtacgc aggttgcgca catagtcttc tgcaccgagg
    12121 gcgataccga tcaaacgctc ggaagcgtga gcgatttcca ctgcgcgggt aatccccagc
    12181 ggagattcaa tcgccgccag caggccggtg ctgccgggtt cacgaccaca ggctttttcg
    12241 atacgcagga tctctttttc gatatccaga acatcctgag cggtatcggt tttcggcaga
    12301 cgcacaacgt ccgcaccacc gcgaacgacg gcttccaggt cgttgacacc ccattcggaa
    12361 tccagcgcgt tgacacgcac aatggtttca atatcgcgat acagcggatg ttgcagcgcg
    12421 tggtaaacca tgcggcgggc ggtgtctttt tcacgcaatg ctacggagtc ttcgaggtca
    12481 aacatcaggg catcagccgg gtagatgaag gagttgctga ccatcgcggc attggcacca
    12541 ggcacaaaca acatgctgcg gcgggtgcga gttttacgtt gttgcagcga agcggaaatc
    12601 attggcaatc ctcccatggc agagccggga taccgctggc gcgtgccagc agggcttcca
    12661 gtcgtgcacg taaaatgcag tccagtgcgc ctttgtcatc gacattcagc tgtacgccgc
    12721 gcacgttgta gcgggcgaga acgtccagaa tggtggtgcg aattgcatcg ccaaactgtt
    12781 tctcaacgct gctattgatt tgcaggtcga tatcctgcgt atcgagtggg gcgatgcgta
    12841 tcatcacatc cccagactca agggtgcctg caacggcggg ctggtttatt ttcatttttc
    12901 acctgtttct catgcggggg tcttttgacg agctgccgcg tcctggcggg agtgctcaag
    12961 caggttctgc aaataatgca gcgtgactgc agggaccagc ggcgcgatag ccgtgagatc
    13021 gtttttcgcc agcagttgac gtacccggga agcggatatc ggcatctcct ggtaacgcag
    13081 ccgctcaatt tcaaccagtt cgatgggcgg tgcggagata gtcggcgttt ccagccagta
    13141 gcgcatatcc tggttgtact gggcggtaac gcgacaaaag ggttcagtac cgacaaagcg
    13201 gtgagttaca cccagcgcgg gagcgaggta ctgacggaaa atcttcagat caatttcggt
    13261 gtaacaatgg ttaatgacgc tctgttcttt aatgaagtag caagggaacg tagcgcggga
    13321 gatgatgtat tcggagccgc gatgcacagt caggcgtgga atatcggcgg tgccttttaa
    13381 caccagatcc aaccggtctt cataggggaa gcgtgaagaa tcttctttga ctaaaaacag
    13441 atgcaaccag tcgcactgtg ccgcagcttg ttgaatcaga taacggtgac cattcgtgaa
    13501 gggattggcg ttcatcacaa tgcagccaat cttgttccct ggatgacgaa attttttcag
    13561 cgattcggca tagcgtttca gccgcgtggc gctgttttcc atcagcacca tcacgccggg
    13621 tacgctggtc agcgtggaaa aaccgcactg gcggaacagc gcctcgtatt cggttttggt
    13681 ataaataaac agatgcgtgc tgtgccgctc ataggcgagg tttatcaatt cagtggctaa
    13741 tgtcagcgcc agtccttcac cgcgaacgga ttcactgata gcaacgcatt taataatatt
    13801 tccggca
//
LOCUS       DAZOYL010000090        13794 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZOYL010000090 DAZOYL010000000
VERSION     DAZOYL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.090"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..147)
                     /locus_tag="ACHUOY_001938"
     CDS             complement(<1..147)
                     /locus_tag="ACHUOY_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR0682727.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="ACHUOY_001939"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="ACHUOY_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="ACHUOY_001940"
                     /pseudo
     CDS             476..1021
                     /locus_tag="ACHUOY_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="ACHUOY_001941"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="ACHUOY_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0682728.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="ACHUOY_001942"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="ACHUOY_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR0682729.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="ACHUOY_001943"
     CDS             3028..3201
                     /locus_tag="ACHUOY_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0682730.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            3227..3295
                     /gene="yncP"
                     /locus_tag="ACHUOY_001944"
     CDS             3227..3295
                     /gene="yncP"
                     /locus_tag="ACHUOY_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR0682731.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="ACHUOY_001945"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="ACHUOY_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR0682732.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="ACHUOY_001946"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="ACHUOY_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR0682733.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="ACHUOY_001947"
     CDS             complement(5499..6422)
                     /locus_tag="ACHUOY_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0682734.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="ACHUOY_001948"
     CDS             6639..7982
                     /locus_tag="ACHUOY_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0682735.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="ACHUOY_001949"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="ACHUOY_001949"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR0682736.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="ACHUOY_001950"
     CDS             10002..10226
                     /locus_tag="ACHUOY_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR0682737.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="ACHUOY_001951"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="ACHUOY_001951"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR0682738.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /gene="ydcK"
                     /locus_tag="ACHUOY_001952"
     CDS             complement(10820..11800)
                     /gene="ydcK"
                     /locus_tag="ACHUOY_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR0682739.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="ACHUOY_001953"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="ACHUOY_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR0682740.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="ACHUOY_001954"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="ACHUOY_001954"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR0682741.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3196 c         3483 g         3575 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gcgcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgttggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catc
//
LOCUS       DAZOYL010000091        13694 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZOYL010000091 DAZOYL010000000
VERSION     DAZOYL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.091"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..77)
                     /gene="yojO"
                     /locus_tag="ACHUOY_001955"
     CDS             complement(<1..77)
                     /gene="yojO"
                     /locus_tag="ACHUOY_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR0682742.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFA"
     gene            186..680
                     /gene="napF"
                     /locus_tag="ACHUOY_001956"
     CDS             186..680
                     /gene="napF"
                     /locus_tag="ACHUOY_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR0682743.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            670..933
                     /gene="napD"
                     /locus_tag="ACHUOY_001957"
     CDS             670..933
                     /gene="napD"
                     /locus_tag="ACHUOY_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR0682744.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            930..3416
                     /gene="napA"
                     /locus_tag="ACHUOY_001958"
     CDS             930..3416
                     /gene="napA"
                     /locus_tag="ACHUOY_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR0682745.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3423..4118
                     /gene="napG"
                     /locus_tag="ACHUOY_001959"
     CDS             3423..4118
                     /gene="napG"
                     /locus_tag="ACHUOY_001959"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR0682746.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4105..4968
                     /gene="napH"
                     /locus_tag="ACHUOY_001960"
     CDS             4105..4968
                     /gene="napH"
                     /locus_tag="ACHUOY_001960"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR0682747.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4965..5414
                     /gene="napB"
                     /locus_tag="ACHUOY_001961"
     CDS             4965..5414
                     /gene="napB"
                     /locus_tag="ACHUOY_001961"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR0682748.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5424..6026
                     /gene="napC"
                     /locus_tag="ACHUOY_001962"
     CDS             5424..6026
                     /gene="napC"
                     /locus_tag="ACHUOY_001962"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR0682749.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6039..6662
                     /gene="ccmA"
                     /locus_tag="ACHUOY_001963"
     CDS             6039..6662
                     /gene="ccmA"
                     /locus_tag="ACHUOY_001963"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR0682750.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6659..7321
                     /gene="ccmB"
                     /locus_tag="ACHUOY_001964"
     CDS             6659..7321
                     /gene="ccmB"
                     /locus_tag="ACHUOY_001964"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR0682751.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7363..8100
                     /gene="ccmC"
                     /locus_tag="ACHUOY_001965"
     CDS             7363..8100
                     /gene="ccmC"
                     /locus_tag="ACHUOY_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR0682752.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8097..8306
                     /gene="ccmD"
                     /locus_tag="ACHUOY_001966"
     CDS             8097..8306
                     /gene="ccmD"
                     /locus_tag="ACHUOY_001966"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR0682753.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8303..8782
                     /gene="ccmE"
                     /locus_tag="ACHUOY_001967"
     CDS             8303..8782
                     /gene="ccmE"
                     /locus_tag="ACHUOY_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR0682754.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8779..10722
                     /gene="ccmF"
                     /locus_tag="ACHUOY_001968"
     CDS             8779..10722
                     /gene="ccmF"
                     /locus_tag="ACHUOY_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR0682755.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10719..11276
                     /gene="dsbE"
                     /locus_tag="ACHUOY_001969"
     CDS             10719..11276
                     /gene="dsbE"
                     /locus_tag="ACHUOY_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR0682756.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11273..12325
                     /gene="ccmH"
                     /locus_tag="ACHUOY_001970"
     CDS             11273..12325
                     /gene="ccmH"
                     /locus_tag="ACHUOY_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR0682757.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12360..13007)
                     /gene="narP"
                     /locus_tag="ACHUOY_001971"
     CDS             complement(12360..13007)
                     /gene="narP"
                     /locus_tag="ACHUOY_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR0682758.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13327..>13694
                     /locus_tag="ACHUOY_001972"
     CDS             13327..>13694
                     /locus_tag="ACHUOY_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0682759.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         2921 a         3454 c         4028 g         3291 t
ORIGIN      
        1 gcaaaagccc gtttttgcca cgcttcccgc cactcttttg atcctgctac aggttttacc
       61 ccgatcgggg tatgcatctt tgacacatcc tttaatatct tagcggctat aaaaatggct
      121 tattaattat gcggtttatt tggtcgctct caattttcag agcgcgttaa tgatggaagg
      181 tcaatgtgaa gattgatgca tcccgtcggg gcatactcac tggtcgctgg cgcaaagcca
      241 gtaacggtat ccgtccgccc tggtccggtg atgaatctca ttttctgacc cattgcaccc
      301 gttgcgacgc ctgtattaat gcttgtgaaa acaacattct gcaacgcggc gcgggcggct
      361 atccgagcgt taatttcaaa aataacgagt gcagcttctg ttacgcctgc gcgcaggctt
      421 gccccgaatc gctattttct ccgcgccaca ccagggcctg ggatttgcag ttcaccattg
      481 gggacgcgtg cctggcgtat cagtcagttg aatgccgccg ctgtcaggat agctgtgagc
      541 caatggcaat tatctttcgc ccgacgctct ccgggatcta ccagccgcaa cttaatagcc
      601 aactttgcaa cggatgtgga gcatgtgcag ccagctgtcc ggtatcagcc ataactgcgg
      661 agtatctcca tgcacactaa ctggcaagtt tgcagcctgg tcgtgcaggc caaaagcgaa
      721 cgaatttcag acatcagcac ccaactgaac gcctttcccg gctgtgaagt tgctgtcagc
      781 gacgcgccga gcggtcagtt gattgtggtg gtggaagcag aagacagcga aacgctgatc
      841 caaaccattg agtcagtacg caacgtagag ggcgtgctgg cggtgtcgct ggtttatcac
      901 cagcaggaag agcaaggtga ggaaacacca tgaaactcag tcgtcgtagc tttatgaaag
      961 ctaacgccgt tgcggccgct gcggcggctg ccggtctcag cgtgccgggc gttgcccgcg
     1021 ccgttgttgg tcagcaggaa gccattaaat gggataaagc gccgtgccgt ttctgcggta
     1081 ctggttgcgg cgttctggtc ggaacgcagc aggggcgtgt ggtggcctgt cagggcgacc
     1141 cggacgcacc ggttaaccgt ggcctgaact gcattaaggg ctatttcctg cccaagatca
     1201 tgtacggtaa agaccgtttg acgcagccgc tgctgcgtat gaaaaacggt aaatatgaca
     1261 aagaaggcga atttacccca atcacctggg atcaggcctt cgatgtgatg gaagagaaat
     1321 tcaaaaccgc cctgaaagaa aaagggccgg aatcgatcgg tatgttcggt tctggtcagt
     1381 ggactatctg ggaaggttat gccgcgtcca agctgtttaa agcgggcttc cgttcgaaca
     1441 acatcgaccc gaacgcgcgt cactgtatgg cgtcggcagt agttggcttt atgcgtacct
     1501 ttggtatgga tgagccgatg ggctgctatg acgacatcga gcaggctgac gcgtttgtgc
     1561 tgtggggcgc aaacatggcg gagatgcacc cgatcctctg gtcgcgcatc actaaccgtc
     1621 gtctctctaa tcagaacgtc accgtggcgg tgctttctac ctaccagcat cgtagcttcg
     1681 agctggcgga taacggcatc atctttacgc cacagtctga cctggtgatc ctgaactaca
     1741 tcgccaacta tatcattcaa aacaatgcga taaatcagga cttcttcagc aaacacgtta
     1801 acctgcgcaa aggggcgacg gacatcggct acggtttacg tccgacccat ccgctggaaa
     1861 aagcagcgaa gaatccaggt tctgacgcct ccgaaccgat gagctttgaa gattacaaag
     1921 ccttcgttgc cgagtatacg ctggaaaaaa ccgctgaaat gaccggcgtg ccgaaagacc
     1981 agttagaaca actggcgcag ctgtatgccg atccgaacaa gaaagtcatc tcctactgga
     2041 cgatgggctt caaccagcat actcgtggcg tgtgggctaa caacctggtc tacaacctgc
     2101 acctgctgac cggcaaaatt tcccagccgg gttgcggtcc gttctccctg accgggcaac
     2161 cttctgcgtg tggtactgcg cgtgaagtgg gcacctttgc tcaccgtctg cctgcggaca
     2221 tggtggtgac caacgagaaa caccgtgata tctgcgagaa gaagtggaat atcccgagcg
     2281 gcaccattcc ggcgaaaatc ggtctgcacg cggtggcaca agaccgtgcg ctgaaagacg
     2341 gcaaactgaa tgtttactgg accatgtgta ccaacaacat gcaggccggg ccgaacatca
     2401 atgaagagcg tatgccgggc tggcgcgatc cgcgcaactt catcatcgtc tccgatccgt
     2461 atccgacagt cagtgcgctg gccgccgact tgatcctgcc gaccgcaatg tgggtagaga
     2521 aagagggtgc ttacggtaac gccgaacgcc gtactcagtt ctggcgtcag caggtacagg
     2581 caccgggcga agcgaaatcg gatctctggc agttagtcca gttctcccgc cgcttcaaaa
     2641 ctgaagaagt gtggccggaa gagctgctag cgaagaaacc ggaactgcgt ggcaaaacgc
     2701 tgtacgaagt gctttatgcc acaccggaag tgagcaaatt cccggtatcc gaactggcgg
     2761 aagatcagct gaacgatgaa tcccgcgagc tgggcttcta tctgcaaaaa gggctgttcg
     2821 aagagtacgc atggtttggt cgcggtcacg gtcacgatct cgcgccgttc gatgactacc
     2881 acaaagcgcg cggtctgcgc tggccggtgg tgaacggtaa agaaacgcag tggcgttaca
     2941 gcgaaggtaa cgacccgtac gtgaaagcgg gcgaaggcta taagttctac ggtaaaccgg
     3001 atggtaaagc ggtgatcttc gcgctgccgt tcgaaccggc ggcggaagca ccggatgaag
     3061 agtacgacct gtggctctct accggacgcg ttctggagca ctggcacact ggcagtatga
     3121 ctcgccgtgt gccggaactg caccgcgcct tcccggaagc ggtcttgttt attcacccgc
     3181 tggatgcgaa agcgcgcgat ctgcgccgtg gcgacaaagt gaaagtggtt tctcgccgtg
     3241 gcgaagtgat ctcgattgtt gaaacgcgcg gtcgtaaccg tccgccacag ggcctggtgt
     3301 acatgccgtt cttcgacgcc gcacagctgg ttaacaaact aacgctggac gcgaccgatc
     3361 cgctctcgaa agagacggac ttcaagaagt gcgcagtcaa actggagaag gtgtaagcca
     3421 ttatgtcccg gtcagcgaaa cctcaaaatg gtcgccgccg ctttctgcgc gatgttgttc
     3481 gcacagcagg cgggctggct gccgtgggtg tggcgctggg gttacaacag caaaccgcac
     3541 gcgcatctgg cgtgcggttg cgcccgcccg gagccataaa cgagaacgcc tttgccagtg
     3601 cctgtgtgcg ttgcggtcag tgtgttcagg cttgccctta cgacacctta aaactggcga
     3661 cgctggcttc tggcctgtcg gcgggcacgc catattttgt cgcacgggat attccttgcg
     3721 aaatgtgtga ggacattccg tgcgccaaag tgtgcccgag tggtgcgctg gatcgtgaga
     3781 tcgaatcgat cgacgacgcg cggatggggc tggcggtgct ggtggaccag gaaaactgtc
     3841 tcaactttca ggggctgcgc tgcgatgttt gttatcgcga atgcccgaaa attgatgagg
     3901 ccatcaccct ggagctggag cgcaacacgc gtaccggtaa gcacgcccgc tttctgccga
     3961 cggttcacag cgacgcctgt accggctgcg gtaagtgcga aaaagtgtgc gtgctggaac
     4021 aaccggcaat caaggtgtta ccgctgtcac tggcgaaagg ggagttaggt caccattacc
     4081 gcttcggctg gctggagggg aacaatggca aatcgtaaac gtgacgccgg gcgcgaggcg
     4141 ctggtgaaaa aaggctggtg gcgcagtcac cgttggctgg tgttacgtcg cctttgtcag
     4201 ttcttcgtgc tggggatgtt tttgagcggt ccgtggtttg gtgtgtggat cttacacggc
     4261 aactacagct gtagcctgtt attcgacacc gttccgctga ccgatccgct gatgacgctg
     4321 caaagtcttg ccagtggtca tctgcctgcc acggtggcgt tgaccggtgc ggtgataatc
     4381 actgtgctct acgccctggc aggaaagcga ttattttgca gctgggtctg cccgctgaac
     4441 ccgattaccg acttagcgaa ctggttacgc aggcgctttg acctcaatca gtctgcgacg
     4501 atccctcgcc acattcgcta cgtgctgttg gtggtcattc tgctgggatc agccttaacc
     4561 ggcacgctca tctgggaatg gatcaacccc gtttccctga tggggcgtag cctggtaatg
     4621 ggcttcggca gcggcgcgct gcttattctc gcgctgtttt tatttgatct actggtcgtt
     4681 gaacacggct ggtgcgggca catttgtccg gttggcgcgc tgtatggcgt gctgggtagc
     4741 aaaggtgtga ttaccgttgc ggccagcgat cggcagaaat gtaaccgctg tatggattgt
     4801 tttcatgttt gcccggaacc gcatgtgcta cgtgccccgg tgctggatga acaaagcccg
     4861 gtgcaggtca ccagccgcga ttgcatgacg tgcggtcgct gcgtggatgt ctgttctgag
     4921 gatgtattta caataactac acgatggagt tcgggagcga aatcatgaaa agccatgacc
     4981 tgaagaaagc gctgtgtcaa tggacggcga tgctggccct ggtggtaagc ggcgcggttt
     5041 gggcggctaa tggcgtcgat tttagccagt cgccggaagt atccgggacg caggaagggg
     5101 ccattcggat gccaaaagag caggatcgga tgccgctgaa ctatgtgaat cagccgccaa
     5161 tgatcccgca tagcgttgaa ggttatcagg taacgaccaa taccaaccgc tgcttgcagt
     5221 gccacggtgt cgaaagctat cgcaccactg gcgcgccgcg tatcagtcct actcacttta
     5281 tggacagcga cggcaaagtg ggcgcggaag tggctccgcg tcgttatttc tgtctgcaat
     5341 gtcacgtacc gcaggccgat accgcgccaa tcgtggggaa tacctttacc ccatcaaaag
     5401 gttacgggaa ataagaggtt attatgggaa attctgaccg taagcctggt ctgattaagc
     5461 gcctgtggaa atggtggcgt acccccagcc gtctggcgct gggaacgctg ctgttgatcg
     5521 gttttgtcgg cggcatcgtc ttctggggcg gctttaacac cggaatggaa aaagccaata
     5581 ccgaagagtt ctgcattagc tgccacgaaa tgcgtaacac ggtgtatcag gaatacatgg
     5641 attccgtgca ctacaacaac cgtagcggcg tccgtgcgac ctgtccggat tgtcacgttc
     5701 cgcacgagtt tgtgccgaag atgatacgca agctcaaagc aagtaaagag ctgtatggta
     5761 aaatttttgg cgttattgac acgccgcaga aatttgaagc tcatcgtctg acaatggcac
     5821 agaatgagtg gcggcgcatg aaggacaata actcgcagga gtgccgtaac tgtcacaact
     5881 tcgagtatat ggatacaacc gcccagaaat cggttgccgc gaagatgcat gaccaggcgg
     5941 tgaaagatgg gcaaacctgt attgattgcc ataaagggat agcgcacaag ctgcccgata
     6001 tgcgtgaagt cgagccaggt ttttaacagg gttattgcgt gggtatgctt gaagccagag
     6061 agttactttg tgagcgggat gaacgaacct tatttagtgg cttgtcattt acgctgaacg
     6121 caggagagtg ggtacaaatc accggtagca acggcgcggg gaagacaacg cttctccgtt
     6181 tgctgacggg gttgtctcgc cctgacgcag gcgaggttct ctggcaaggg cagcccttgc
     6241 atcaggtacg cgacagctac catcaaaacc tgttatggat aggccatcag ccggggatca
     6301 aaacccggct gacggcgtta gaaaatctgc acttttatca tcgcgatggc gataccgcac
     6361 aatgtctgga agccctggcg caggccgggc ttgccggatt cgacgatatt ccggtaaatc
     6421 aactttctgc cgggcaacaa cgccgcgtcg ctttagcgcg tctgtggctg acccgtgcca
     6481 cgttatggat cctcgacgag ccttttaccg cgattgacgt taacggcgtc gatcgtctga
     6541 cccagcgtat ggcgcagcat acggagcagg gggggattgt gattctgact acccaccagc
     6601 cgctcaacgt tgctgaaagt aaaattcgcc gcatttcact gacgcaaacg agggccgcat
     6661 gatgttctgg cgcattttcc gtcttgagct gcgtgtagcg tttcgccata gcgccgaaat
     6721 cgccaacccg ctgtggttct tcctgattgt aattaccctt tttccgctca gtatcggtcc
     6781 ggagccgcaa ctgctggcgc gtattgcacc gggcattatc tgggttgctg cgctgctttc
     6841 atccttgctg gcgctggaac gactgttccg tgacgatttg caggacggca gtcttgaaca
     6901 attgatgttg ttgccgttac ccttgcccgc cgttgtgctg gcgaaggtga tggcgcactg
     6961 gatggtaacc ggtctgccgt tactcatcct ttcgccactg gtagcaatgc tactgggaat
     7021 ggatgtttat ggctggcaag tgatggcgct gacgctgctg ctgggaacgc ctacgcttgg
     7081 ctttctcggt gcaccgggtg tggcgttgac agtgggactt aagcgcggtg gtgtgctgct
     7141 cagcatactg gtgttaccgc tgactatccc attactcatc tttgccaccg ccgcgatgga
     7201 cgcggcttcc atgcatttgc ccgttgacgg gtatctggca attttaggcg cgttgctggc
     7261 aggcaccgcg acattaagtc cttttgcgac ggcggcagcg ttacgaatca gcattcaata
     7321 acgctggatt actgattttt gttcgagtct ggtatcgaaa ctatgtggaa aacactgcat
     7381 caactggcga tcccaccacg gctgtatcaa atctgtggct ggtttatacc gtggctggca
     7441 attgccagtg tggtcgtgct taccgtcggc tggatctggg gattcggctt tgctccggct
     7501 gattatcagc agggaaatag ctaccgcatt atctacctgc atgtgcctgc ggcgatctgg
     7561 tcgatgggca tttatgcatc aatggcagtg gcagcgttta ttggccttgt ctggcagatg
     7621 aaaatggcca acctggcggt ggcggcgatg gcccccattg gtgccgtgtt tacctttatt
     7681 gccctggtta ccggctctgc atggggaaaa ccgatgtggg gcacctggtg ggtatgggat
     7741 gcacgtctga cttctgaact ggtgctgctg tttttgtatg tgggtgtgat tgccctgtgg
     7801 cacgccttcg acgaccgccg tctggcgggc cgtgcggcag gtatcctggt gctgattggc
     7861 gtggtgaatc tgccgattat tcattactcc gtggagtggt ggaacaccct gcatcaggga
     7921 tcaacgcgga tgcagcaaag tatcgatccg gcgatgcgtt cgccgctgcg ctggtcgatt
     7981 tttggcttcc tgctcctgtc tgccacgctg acgctgatgc ggatgcgtaa tttgattttg
     8041 ctgatggaaa aacgccgtcc gtgggtgagt gaactgatac tgaaaagagg ccgtaaatga
     8101 cccctgcatt tgcttcctgg aatgaatttt tcgcaatggg cggttacgcc ttttttgtct
     8161 ggctggcggt ggtgatgacc gttattccgc tggtggtttt ggtcgtgcac tcggtgatgc
     8221 aacatcgcgc aattctgcgt ggtgtggcgc aacagcgggc gcgtgaggcg cgtttacgtg
     8281 ctgcgcaaca gcaggaggct gcatgaatat tcgccgtaaa aaccgcttgt ggattgcctg
     8341 tgccgtgttg gcagggctgg cgctgactat cggtctggtg ctatatgcgc tgcgctcgaa
     8401 tatcgatctc ttttatacgc cgggggaaat tctctacggc aagcgtgaaa ctcagcaaat
     8461 gccggaagtc ggtcagcgtt tgcgcgttgg cgggatggtg atgctgggta gtgtgcagcg
     8521 cgatcccaat tcgctgaaag tgaccttcac tatttacgat gctgaaggct cagtggatgt
     8581 ctcttacgaa ggcattttgc cggatctgtt ccgtgaaggg cagggcgttg tggtgcaggg
     8641 cgagctggaa aaaggcaatc atatcctcgc gaaagaagtg ctggcgaaac acgacgaaaa
     8701 ctacacgccg ccagaagttg agaaagcgat ggaagctaac caccgtcgcc cggcgagtgt
     8761 ttataaggac ccagcatcat gatgccggaa attggaaacg ggctgctgtg tctggcgtta
     8821 gggattgcgc tgctgctgtc cgtgtatccg ctatggggcg tggcgcgcgg agatgcgcgc
     8881 atgatcgcgt cttcccgctt gtttgcctgg ctgctgttta tgtctgtggc tggcgcattt
     8941 ctggtgctgg tcaatgcttt cgtggtcaac gactttaccg tcacctatgt tgccagctac
     9001 tccaataccc agcttccggt gtggtatcgc gtggcggcta cctggggcgc gcatgaaggc
     9061 tcgctactgc tgtgggtgct gctgatgagc ggctggacct ttgcggtggc gatttttagt
     9121 cagcgtattc cgctggatat tgtggcccgc gtactggcga taatggggat ggtcagcgtc
     9181 ggctttttgc tgttcattct ctttacctct aacccattct cacgcacgct gccgaacttc
     9241 ccgattgaag ggcgcgatct taacccgctg ttacaggacc cggggctgat cttccatccg
     9301 cctctgcttt atatggggta cgtgggtttc tcggtggcgt ttgcttttgc cattgcttct
     9361 ttgctgagcg ggcgtctgga cagcacttat gcgcgtttta ctcgcccgtg gacgctggcg
     9421 gcgtggattt tcctgacgct cggcattgtg ctcggttccg catgggccta ttacgaactc
     9481 ggctgggggg gctggtggtt ctgggatccg gtagaaaacg cctcgtttat gccgtggctg
     9541 gtggggactg cgctgatgca ctcactggcg gtcactgaac aacgcgccag cttcaaagcg
     9601 tggacattac tgctggcaat cagtgccttc tcgttgtgtc tgttggggac tttcctcgtg
     9661 cgttccggcg tgctggtatc ggtacacgcg tttgcgtctg atccggcgcg cggtatgttt
     9721 atcctcgcct ttatggtgct ggtgattgga ggttcgctgc tgctgtttgc cgcgcgtgga
     9781 cacaaagttc gctcacgcgt aaacaatgcg ctgtggtcgc gggaatctct gctgttagcg
     9841 aacaacgttt tgctggtcgc cgcgatgctg gtggtgttgc tggggacgct gctgccgctg
     9901 gtgcacaagc aactgggact gggcagtatt tcgattggcg aaccgttctt caacaccatg
     9961 tttacctggc tgatggtgcc gtttgcgcta ctgctgggtg tcggtcctct ggtgcgctgg
    10021 gggcgcgatc gcccacgtaa aatccgcaat ttattgatta tcgccttcat ctcaacgctg
    10081 gtgctgtctc tgctgttgcc gtggctgttt gagagcaaag ttgtggcgat gacggtgctc
    10141 ggcctggcaa tggcctgctg gattgcggtg ctggcaattg cggaagctgc gctgcgtatt
    10201 tcacgcggca cgaaaaccac cttcagttat tgggggatgg tggctgctca cctggggctg
    10261 gcagtgacaa ttgttggcat tgcttttagc cagaactata gcgttgagcg tgatgtgcgc
    10321 atgaagtccg gcgatagcat cgatattcat gaatatcgct tcaccttccg tgatgtcaaa
    10381 gaggtgactg gcccgaactg gcgtggcggt gtggcgacta tcggcgtaac gcgagagggt
    10441 aaaccggaaa cagtgctgta tgcggaaaaa cgttattaca acactgccgg gtcgatgatg
    10501 accgaagcgg cgattgacgg tggcatcacg cgtgacctgt acgccgcgct cggtgaagag
    10561 ctggaaaacg gcgcgtgggc tgtgcgtctt tactacaaac catttgttcg ctggatttgg
    10621 gcgggcgggc tgatgatggc gttgggcgga ctgctgtgtc tgtttgatcc tcgctatcgt
    10681 aagcgcgtga gtccgcaaaa aactgcgccg gaggctgtat gaagcgcaaa gtattgttaa
    10741 ttccgttgat tatcttcctg gcgattgccg cggcgctgct gtggcagctg gcgcgtaatg
    10801 ccgaagggga tgatccgacc aatctggaat cggcgctcat tggcaagcca gtgccgaagt
    10861 ttcgcctcga atcactggac aatccggggc agttttatca ggcggatgtg ctgactcagg
    10921 gcaaaccagt gctgcttaac gtctgggcga cctggtgtcc gacctgccgt gcggaacatc
    10981 aatatctgaa tcagctttct gcgcagggca tccgcgtagt cggcatgaac tataaagacg
    11041 atcgccagaa ggcaatcagc tggctgaaag agctgggcaa tccttacgcg ctaagcctgt
    11101 ttgatggcga tggcatgtta gggctggatc tcggtgtcta tggcgcgcca gaaacgttcc
    11161 ttattgacgg caacggcatc attcgctatc gccatgcggg cgatctcaat cctcgcgtct
    11221 gggaagaaga gatcaagccg ctgtgggaga aatacagtaa ggaggccgca caatgaggtt
    11281 tttattgggc atgctgatgc tgatgatctc cggctcagcg ctggcgacca tagacgtgtt
    11341 gcagtttaaa gatgaagcac aggaacaaca gttccgtcag ctcactgaag aactgcgctg
    11401 cccgaaatgc cagaacaaca gcattgccga ttccaactcg atgattgcca ccgacctgcg
    11461 ccagaaagtg tatgaactga tgcaggaagg taaaagtaaa aaagagattg tcgattatat
    11521 ggtggcgcgt tacggcaact tcgtcactta cgatccgccg ttaacgccgc tgaccgtgct
    11581 gctgtgggtg ctgccagtag tagctattgg cattggcggt tgggtcatat acgcccgctc
    11641 gcggcgtcgg gtacgcgtgg tgccggaagc gtttcctgaa caaagcgtgc cggaaggtaa
    11701 gcgtgccgga tatgtcgctt atttgccggg cattgtggtg gcgttaattg tggctggcgt
    11761 cagctactac cagaccggaa attatcaaca ggtgaaaatc tggcagcagg ccacggcaca
    11821 ggctccggcg ttgctggaca gggcgctgga tccgaaagct gatccgctca acgaagaaga
    11881 gatgtcgcgc cttgcgctgg ggatgcgtac tcaactgcaa aaaaatccgg gagatataga
    11941 cggctggatt atgttgggcc gcgttggcat ggcgctgggt aacgccagta tcgccaccga
    12001 tgcatacgct actgcgtatc gcctcgatcc gaaaaacagt gatgccgcac tgggttatgc
    12061 tgaagcgttg acacgttcat ctgatcccaa cgacaaccgc ctcggcggcg aactgctacg
    12121 tcagctggtg agaacggacc atagcaatat ccgtgtgcta agcatgtatg cgtttaatgc
    12181 ctttgagcag cagcgatttg gcgaagccgt tgccgcgtgg gagatgatgt tgaaactctt
    12241 acctgccaac gatactcgcc gtgcggtgat tgaacgtagt atcgcgcagg cgatgcaaca
    12301 tttgtcgccg caggagagta aataaaaaga ggcccgatgg tgatgccatc gggctatttt
    12361 tattgtgccc cgcgttgttg caggaacaga atggtggccg ccacgcgtga gcggacattg
    12421 agtttacgca gcagatttcg aatatgaact tttactgtct gctcggaaat attcaacaca
    12481 gaggcaatct gtttatttga cagcccctgt gccagctcgt gcagaacatc cagctcgcgc
    12541 tccgtcagca cgctgaaggg atcttcttcc gcgccaaaca tttcacgttc acgtaagtac
    12601 tgattgacgc gttcgctaaa gactttgctg cctttcgctc cggcacgaat cgcttccagc
    12661 aacacttccg ggtcgctgtc tttcagcaga taaccgtctg cgcctgcgtc tatcagcgca
    12721 aagacatcgc tggaggcatc ggatacggtc aggataataa tttgcgcggt aacgccatcc
    12781 ctgcgcaagg cattgagggt atccaggcca ctcatacctt tcatattgag atccagcaag
    12841 atcacgtcga tatccagtct attcgccaga tcgatcgcgc tcgcgccgtc gcccgcttcg
    12901 gcgaccactt caaagccagg atcaagctcc agtaactgac gaacaccgcg tcgcataagt
    12961 ggatgatcat ccacaatcat cacctgaaaa ggcgttgctt caggcatagt agtctcctga
    13021 ggttttatta gaatcattat gtttgagcgt agcgttggta acgctaccag gcgcgtgaat
    13081 gttaccccat tttatgtatg gacatgtagt gaaaactaca accttgccgt tcgctttggc
    13141 atcattttca ttataaagac ggaattttgc atcagcttct catcaagaaa aacaactaaa
    13201 aaatcaatca caatacttgt gcatttcttt aaacagtttt taaaaactga ctccgggtat
    13261 ggagctatgg gtattttctg tacccaatgc ttttaacagc aattaatttc ataggatgaa
    13321 agctcaatgc atcaatctgg ttctgtttct ctttgtcgtt ccgcaatatc tgttctggtg
    13381 gctacagcgt tatattcacc catagcattg gcatcaacag ttgagtatgg tgagacagtt
    13441 gatggtgttg gcctggaaaa agatatccag ctggtttatg ggaccgccaa taatacgaaa
    13501 atcaatcctg gcggagaaca gcatataaaa gaatttggtg taagtagtaa tactgaaatt
    13561 aacggcgggt atcagtacat tgaaatgaat ggcactgcag aatactcagt attaaatgat
    13621 ggttatcaaa ttgttcaaat gggtggcgcg gtaaaccaga ctacgctcaa taatggagtg
    13681 ctacaggttt atgg
//
LOCUS       DAZOYL010000092        13498 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZOYL010000092 DAZOYL010000000
VERSION     DAZOYL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13498
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.092"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(36..119)
                     /locus_tag="ACHUOY_001973"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="ACHUOY_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHUOY_001974"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHUOY_001974"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR0682760.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHUOY_001975"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHUOY_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR0682761.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHUOY_001976"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHUOY_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR0682762.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="ACHUOY_001977"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="ACHUOY_001977"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR0682763.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="ACHUOY_001978"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="ACHUOY_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR0682764.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="ACHUOY_001979"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="ACHUOY_001979"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR0682765.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="ACHUOY_001980"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="ACHUOY_001980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR0682766.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="ACHUOY_001981"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="ACHUOY_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHUOY_001982"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHUOY_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR0682767.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="ACHUOY_001983"
     CDS             complement(12425..12664)
                     /locus_tag="ACHUOY_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR0682768.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHUOY_001984"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHUOY_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR0682769.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13041..13328
                     /locus_tag="ACHUOY_001985"
     CDS             13041..13328
                     /locus_tag="ACHUOY_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR0682770.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3520 a         3321 c         3468 g         3189 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    13021 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    13081 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    13141 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    13201 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    13261 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    13321 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    13381 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    13441 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcatt
//
LOCUS       DAZOYL010000093        13486 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZOYL010000093 DAZOYL010000000
VERSION     DAZOYL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.093"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..2309)
                     /locus_tag="ACHUOY_001986"
     CDS             complement(<1..2309)
                     /locus_tag="ACHUOY_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR0682771.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMG"
     gene            complement(2406..2882)
                     /gene="greB"
                     /locus_tag="ACHUOY_001987"
     CDS             complement(2406..2882)
                     /gene="greB"
                     /locus_tag="ACHUOY_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR0682772.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3110..3829
                     /gene="ompR"
                     /locus_tag="ACHUOY_001988"
     CDS             3110..3829
                     /gene="ompR"
                     /locus_tag="ACHUOY_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR0682773.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3826..5178
                     /gene="envZ"
                     /locus_tag="ACHUOY_001989"
     CDS             3826..5178
                     /gene="envZ"
                     /locus_tag="ACHUOY_001989"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR0682774.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5254..6876)
                     /gene="pckA"
                     /locus_tag="ACHUOY_001990"
     CDS             complement(5254..6876)
                     /gene="pckA"
                     /locus_tag="ACHUOY_001990"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR0682775.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7255..8955
                     /locus_tag="ACHUOY_001991"
                     /pseudo
     CDS             7255..8955
                     /locus_tag="ACHUOY_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(9090..9968)
                     /gene="hslO"
                     /locus_tag="ACHUOY_001992"
     CDS             complement(9090..9968)
                     /gene="hslO"
                     /locus_tag="ACHUOY_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR0682776.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(9993..10394)
                     /gene="hslR"
                     /locus_tag="ACHUOY_001993"
     CDS             complement(9993..10394)
                     /gene="hslR"
                     /locus_tag="ACHUOY_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR0682777.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10405..11073)
                     /gene="yrfG"
                     /locus_tag="ACHUOY_001994"
     CDS             complement(10405..11073)
                     /gene="yrfG"
                     /locus_tag="ACHUOY_001994"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR0682778.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11138..13273)
                     /gene="igaA"
                     /locus_tag="ACHUOY_001995"
     CDS             complement(11138..13273)
                     /gene="igaA"
                     /locus_tag="ACHUOY_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR0682779.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
BASE COUNT         2996 a         3472 c         3625 g         3393 t
ORIGIN      
        1 cccattgccg ccgccagcgc atccatcatc gcgctattac cggcaggctg cgcttcacga
       61 ccgcgtggtt tggctgccgg gcggttgttt tgcgggcgtt cattaccgcc gccgcgacgg
      121 gcgttggttt cgccaggctg ctcgtccaga cgcatggtca gggcgatacg tttacgctga
      181 agatccactt ccagcacttt caccttcaca atgtcgcccg ctttcaccac ggtatgcgga
      241 tcttccacaa acttgttcga caatgaagag atgtgaacca ggccgtcctg atgcacgcca
      301 atatcgacaa acgcgccaaa gttggtgacg ttggtgacag cgccttcgag gatcataccc
      361 ggttgcaggt cgttcattgt ctcgacgcca tcggcaaact gagcggtttt aaattccgga
      421 cgcggatcgc gacccggttt ttccagctct ttgatgatgt cagttactgt cggcacaccg
      481 aatttttcat cagtaaagtc agacgctttc aggttacgca gttcgctgct gttacccatc
      541 agatctttca gtgcctgctg tgttgctgcc agaatgcgtt ccaccaccgg ataggcttcc
      601 gggtgaacgg tagaggcgtc cagcgggtta tcaccgtggt taatgcgcag gaagcccgcg
      661 cactgctcga aggctttcgg ccccagacgg ctgactttca gcagttgctg acggttcttg
      721 aactggccgt tctcatcgcg ccaggccacg atgttttgcg ccatcatgcg cgtcaggccc
      781 gccacgcgag ttaatagcgg aacagaagcg gtgttgagat cgacgccaac ggcgtttacg
      841 cagtcttcta ctactgcatc cagtttgcgg gccagttgcg tctggctgac gtcatgctga
      901 tactgaccta cgccgataga tttcggatcg attttcacca gctccgccag cggatcctgc
      961 aaacggcggg cgatagatac cgcaccacgc agcgaaacgt cgagatccgg gaactcctgc
     1021 gctgccagct cggaagctga gtaaaccgac gcgccagctt cgctgacgat cactttctgt
     1081 gcggtcactt tcgggaactg cttctgcacg tcgagataga aacgttcagt ttcgcgggaa
     1141 gctgtaccgt tgccgatcgc caccagttca acgttatgtt tttcacacaa ggcagcaacg
     1201 gtcatcgcta cttttgcggc ttgtccggtg tgcgggtaaa tggtatcggt cgccaccagt
     1261 ttgccagtgg catcgaccac tgccactttt accccggtac gcagacccgg atcgaggccc
     1321 atcgttgcac gcagtccggc aggggccgcc atcagcagat cgtgcaggtt acgggcaaag
     1381 acgttgattg cttcatcttc cgcgcgttcg cgcacggtac ccatcagttc ggtttccaga
     1441 tgcatcagca ccttgatgcg ccaggtccag ctcactacgc ctttgcgcca gctatccgcc
     1501 ggggcattgt tcaggcgcag gccaaggtga tccatgatga tttgctcgca atagctctct
     1561 ttgggcggct catcgaactg cggatcggca ttcagcgaaa gctggagtac gccttcgtta
     1621 cgcccacgga acatcgccag cgcgcggtga gaaggcaccg tggacaacgg ttcgtgatga
     1681 tcgaaatagt cgcggaattt cgccccttcc tcttctttac cgctcaccac cgtagaaacc
     1741 aaatgcgcgt tcttccacag ataatcacgc actttcgcca gcagcgcggc atcttcggca
     1801 aaccgttcca tcaggatata gcgcgcgccg tccagcgcgg ctttggtatc tgccacgcct
     1861 ttatcggcat caatatattg cgcagcggcg acttctggtg tgtgtgacgg atcgctccac
     1921 agcaggtcag ccaacggctc aagccctgct tcaatggcga tttgcccgcg ggtgcggcgt
     1981 ttaggtttgt agggcaggta gaggtcttcg agttcggttt tgcttagggt ggcgttgatg
     2041 gccttcgcca gatcatcggt gagtttgcct tgctcggaaa tggacttgag gatcgcctga
     2101 cgtctctctt ccagctcgcg cagatagctc agacgcgttt ccagattacg cagctgcgtg
     2161 tcatccagac cgccggtgat ttccttacga taacgtgcga taaacggcac ggtattccct
     2221 tcgtcaagca ggcgaacggc agcgtcaacc tgttccgggc gcgcctgaat ttcacccgca
     2281 ataatgcggc agaacgaatc attcatcatg gtttgagttc atcttttcgg atcaaaaatc
     2341 aggggatagt tatacggact ggctggcaaa aatgccagcc atcggcagga ggttaagact
     2401 cttcattacg gtttcacgta ttcgatagca ttaacatacc agctcgcttc cccggcaggg
     2461 gtattcacca ccgccagatc gccgacttct tttttcagca atgcgcgggc catcggggaa
     2521 tcgatagaga tgtaatcttt acggccaaaa atttcatcgt agccgacaat acggaaacgg
     2581 tgagtcacgc catcgtcgtt ttcaatctcc acccacgcgc caaaaaagac tttgccttcc
     2641 tgctgagggg agtaattgac gattttgaga ttttccatgc atttagtgag atagcgcacg
     2701 cgacggtcga tttcacgcag acgcttttta ttatactgat agtcagcatt ttcgctgcgg
     2761 tcgcccagac ttgcggccca ggtcaccttt tttgtgacct ccgggcgttc ttcacgccag
     2821 agataattaa gctcttgttt gagtttttca tacccttccc gggtaaccag gggcgttttc
     2881 atctcgttga ttccctttgt ctgtttgata atgcgcacat tgggtataac gtgatcatat
     2941 caacagaatc aataatgttt cgccgaataa attgtatact taagctgctg tttaatatgc
     3001 tttgtaacaa tttaggctga aattcatacc agatttagct ggtgacgaac gtgagctttt
     3061 ttaagaatac acgcttacaa attgttgcga acctttggga gtacaaacaa tgcaagagaa
     3121 ctacaagatt ctggtggtcg atgacgacat gcgcctgcgt gcgctgctgg aacgttatct
     3181 caccgaacaa ggcttccagg ttcgaagcgt cgctaatgca gaacagatgg atcgcctgct
     3241 gactcgtgaa tctttccatc ttatggtact ggatttaatg ttacctggtg aagatggctt
     3301 gtcgatttgc cgacgtcttc gtagtcagag caacccgatg ccgatcatta tggtgacggc
     3361 gaaaggggaa gaagtggacc gtatcgtagg cctggagatt ggcgctgacg actacattcc
     3421 aaaaccgttt aacccgcgtg aactgctggc ccgtattcgt gcggtgctgc gtcgtcaggc
     3481 gaacgaactg ccaggcgcac cgtcacagga agaggcggta attgctttcg gtaagttcaa
     3541 acttaacctc ggtacgcgcg aaatgttccg cgaagatgag ccgatgccgc tcaccagcgg
     3601 tgagtttgcg gtactgaagg cactggtcag ccatccgcgt gagccgctct cccgcgataa
     3661 gctgatgaac cttgcccgtg gtcgtgaata ttccgcaatg gaacgctcca tcgacgtgca
     3721 gatttcgcgt ctgcgccgca tggtagaaga agatccagcg catccgcgtt acattcagac
     3781 cgtttggggc ctgggctacg tctttgtacc ggacggctct aaagcatgag gcgattgcgc
     3841 ttctcgccac gaagttcatt tgcccgtacg ttattgctca tcgtcacctt gctgttcgcc
     3901 agcctggtga cgacttatct ggtggtgctg aacttcgcga ttttgccgag cctccagcag
     3961 tttaataaag tcctcgcgta cgaagtgcgt atgttgatga ccgacaaact gcaactggag
     4021 gacggcacgc agttggttgt gcctcccgct ttccgtcggg agatctaccg tgagctgggg
     4081 atctctctct actccaacga ggctgccgaa gaggcaggtc tgcgttgggc gcaacactat
     4141 gaattcttaa gccatcagat ggcacagcaa ctgggcggcc cgacggaagt gcgcgttgag
     4201 gtcaacaaaa gttcgcctgt cgtctggctg aaaacctggc tgtcgcccaa tatctgggta
     4261 cgcgtgccgc tgaccgaaat tcatcagggc gatttctctc cgctgttccg ctatacgctg
     4321 gcgattatgc tattggcgat aggcggggcg tggctgttta ttcgtatcca gaaccgaccg
     4381 ttggtcgatc tcgaacatgc tgctttgcag gtcggtaaag ggattattcc gccgccgctg
     4441 cgtgagtatg gcgcgtccga ggtgcgttcc gttacccgcg cctttaacca tatggcggct
     4501 ggtgttaagc aactggcgga tgaccgcacg ctgctgatgg cgggggtaag tcacgacttg
     4561 cgcacgccgc tgacgcgtat tcgcctggcg actgagatga tgagcgagca ggatggctat
     4621 ctggcagaat cgatcaataa agatatcgaa gagtgcaacg ccatcattga gcagtttatc
     4681 gactacctgc gcaccgggca ggagatgccg atggaaatgg cggatcttaa tgcagtgctc
     4741 ggtgaggtga ttgctgccga aagtggctat gagcgggaaa ttgaaaccgc gctttacccc
     4801 ggcagcattg aagtgaaaat gcacccgctg tcgatcaaac gcgcggtggc gaatatggtg
     4861 gtcaacgccg ctcgttatgg caatggctgg atcaaagtca gcagcggaac ggagccgaat
     4921 cgcgcctggt tccaggtgga agatgacggt ccgggaattg cgccggaaca acgtaagcac
     4981 ctgttccagc cgtttgtccg cggcgacagt gcgcgcacca ttagcggcac gggattaggg
     5041 ctggcaattg tgcagcgtat cgtggataac cataacggga tgctggagct tggcaccagc
     5101 gagcggggcg ggctttccat tcgcgcctgg ctgccagtgc cggtaacgcg ggcgcagggc
     5161 acgacaaaag aagggtaaat aaacgggagg cgaaggtgcc tcccgttttg ctttctataa
     5221 gatactggat agatattctc cagcttcaaa tcattacagt ttcggaccag ccgctaccag
     5281 cgcggcaccc gcaggggtgt cggtgtattt atcgaagttg tcgataaaca gtttcgccag
     5341 ggtttcggct ttttcctgcc actgttccgg agaagcgtag gtgttacgcg gatcgagaat
     5401 cttcgtgtct acgcccggca gttcggttgg gatcgccagg ttaaacatcg gcagagtgaa
     5461 ggtttctgca ttatccagcg aaccgttgag gatggcgtcg ataatggcgc gggtatcttt
     5521 aatcgagata cgtttgccag tgccgttcca gccagtgtta accagataag cctgcgcgcc
     5581 cgccgcctgc atacgtttca ccagcacttc tgcgtactga gtcgggtgca gcgacaggaa
     5641 cgccgcgccg aagcaagcgg agaaggttgg cgtcggttcg gtgatgccac gctcagtacc
     5701 ggccagtttg gcggtgaagc cagagaggaa gtgatactgg gtttgatcgg cagtcaggcg
     5761 agaaaccggc ggcaacacgc cgaaagcatc agcagtcagg aagataacct tagtcgcgtg
     5821 gcccgctttg gaaaccggct taacgatgtt atcgatgtga tagattggat aagaaacgcg
     5881 ggtgttctcg gtttttgaac catcatcaaa gtcgatagtg ccatcttcac gcacggtgac
     5941 gttttccagc agcgcatcac gacggatagc gttgtagatt tcaggttccg cttctttcga
     6001 cagcttgata gtttttgcgt agcagccgcc ttcgaaatta aacacgccgt catcgtccca
     6061 gccgtgttcg tcatcgccaa tcaggcggcg tttcgggtcg gtggaaaggg tggttttacc
     6121 ggtgccggaa aggccgaaga acaccgcaac atcgcctttc tcgccaacgt tggcggagca
     6181 gtgcatagaa gcgatacctt tcagcggcag caggtagttc atcatcgaga acatcccttt
     6241 cttcatttcg ccgccgtacc aggtgccgcc aatcagctgc atgcgctcgg tcaggttaaa
     6301 cgccacgaag ttttcggagt tcagaccctg ttctttccac tgcgggttag tgcacttcgc
     6361 gccgttcata acgataaagt ctggtttgaa acctgccagc tcttcatcgc tcgggcgaat
     6421 aaacatgttt ttgacaaaat gcgcctgcca ggccacttcg gtgatgaaac ggacggaaag
     6481 acgagtatcc gggttcgcac cacagaaagc gtcgacaacg aacagacgtt tgccggaaag
     6541 ctgcctggtc accaggcctt tcagatgctg ccaggtttcc ggagagagag gtttgttgtc
     6601 gttcttacct ttgcctttgt ctgcccacca gaaagtatcg cgagtggtat cgtcacggac
     6661 gatatactta tcttttggtg aacgaccggt gaagatcccg gtatcgacgg caacggcacc
     6721 cagattagtt aacaccccgc gctcataacc tgtcaggctc ggatcgagct cttcctgata
     6781 cagcaggtcg tagcttgggt tgtaaacgat atcatgtacg tcactgatac cataagcctc
     6841 gagttcttgc ggggtcaaac cattgttaac gcgcatttca ctgctcctta gccaatatgt
     6901 attgcctgaa tagtaaagtc tttttggggg tgttaaccgc gacaaggctc atagatttac
     6961 gtatctggag aaattcatat ttgacaaaaa actccgtgat tcctgtcacg aaacggttgc
     7021 tattatcgca gaaatagcat tttaggtgtg gaaaatgttc tcaaaaggtt aaatcttggt
     7081 ttatatggcg ggaatattgt gagtggaatc gcattcctga atgaaattga tgcaaattca
     7141 accggtaaag tatcgattca ccacgctaac tttttttgat tattttctga aaaaagcaaa
     7201 atactactgc ccggatgcga taattaaccc accttttgat tctggataac caacatggat
     7261 aacgttgaac tttcaccggc aacacgctgg gggatgattg ctaccggatt acttcagggg
     7321 ctggtttgct acctgcttat cgcctggctt gccggaaaaa atcacagttg gattgtttat
     7381 ggcgtgcctg cgaccgtcgc gttttcatcc gttttactct tctccgtgat ctcttttaaa
     7441 cagaagcgcc tttggggatg gctggcgctg gtgtttattg ccacgacagg gatgagcggt
     7501 tggctgaagt ggcaaactga tggcatgaac ccctggagag ctgaaaaggc gatttgggat
     7561 tttggctgat atctgctgtt gatggcaatg ttgttgctac cgtggataca acaaagcctt
     7621 cgtatccgca atggcagtag ccgctatagc tacttttacc agtcagtatg gcataacgta
     7681 cttatattac tggtgatttt tctctccaat ggcctgacgt ggctggtgct tttactgtgg
     7741 ggtgagttgt ttaaacttgt tggcatcacg tttttcaata cgctcttttt tgcaaccgac
     7801 tggtttatgt atcttacatt tggtctggtt accgcactgg tggtgatcct cgcgcgtaca
     7861 cagtcacgtt taatcgactc tattcaaaag ttgttcacgc taatcgccac ggggttgctg
     7921 ccgttagtat cattgctaac cctgatgttt atcatcaccc tgccgtttac ggacctgagc
     7981 gcgatttctc gccacatctc cgccgccggg ttgctgttaa cgctggcctt tttgcaattg
     8041 atcttaatgg ctattgtccg cgatccgcaa aaagcgtcac ttccctggac ggggccgttg
     8101 cgttgcctga ttaaaaccgc tttgctggtt gctccgctgt atgtgttcgt cgccgcctgg
     8161 gcgttatggc tgcgggtcgc tcagtacggc tggactgtcg accgcttgca gggcgcgctg
     8221 gcggtgctgg tgttactggt gtggtcgctg gggtattttg tcagtatcgt ctggcgtaat
     8281 gggcaaaatc cccttgttct tcagggcaaa gtgaaccttg cggtttcgtt attggtgttg
     8341 gtgatactgg tgcttcttaa ttcgccggtg ctggacagta tgcgcattag cgtgaacagt
     8401 catatggcgc gttatcagag cggcaaaaac acgccagacc aggtaactat ctacatgctc
     8461 gaacagagcg gtcgctatgg acgtgcggcg cttgagtcgc tgaaaagcga tgccgggttt
     8521 atgaaagacc cgaaacgcgc gcgggatctg ctgatggcgt tagatggaga gcaacatctt
     8581 caggaacagg tatcggaaaa agtattagcc gaaaatgtgt taattgctcc tggttctgtt
     8641 aaaccggatg cgacattctg gtcggcctta atccaggatc gctataacgt gatgacctgt
     8701 attgaaaaag acgcctgcgt cctggtcgag caagatctga atagtgatgg tcaggcggag
     8761 cggatcctgt ttgcttttaa tgatgacaga gtcattgtct atggctttga ctcagacaga
     8821 aaagaatggg acgcgcttga tatgagttta cttccgaacg aaataacgaa agaaaaatta
     8881 ctcacagctg cgaaggatgg aaaactggat ctgacggtag atggtgaaac gctggaaata
     8941 aacttaagta agtgacaact cttttaacgg atacgacgct gtatctgacc aacaataatt
     9001 tgcgatttat ttcacaggcc tggtaagcgc agcacatcag gccattacgt cgtaaaatcc
     9061 ggcagagccc tctctgccgg acatactcat taatgaactt gcggatctgc cggagacgcg
     9121 ttgttgcgga tttcagcaat atccatcgca ttgaacagat agtggttacc gcagtaatca
     9181 caatgcatgt caatttcgcc atcttccgcc aggatgctat cgacttcttc atcaggcagc
     9241 gttttcagcg cgtcggcgca acgttcacgc gagcaggtgc atttgaactc cacatcctgc
     9301 ggatcgtaaa ccgtcacctc ttcttcgtga tacaaacgcc acaacacttc gtttgccggt
     9361 aaggtcagca gttcttcggt tttgatggtt tcggttagcg tcgccaggtg gtcaaagtcg
     9421 tcctgctggg cattttgcgc aggcattacc tgcaacaaca taccgcctgc agccggtttg
     9481 ccgtctacgt cgccggtgcg aataaacagg cgcgtcggca gctgttcaga acgcataaag
     9541 taatcttcca ggcaggccgc cagggtatca ccttccagac caaccacgcc ctgatagcgt
     9601 tcgccttcgc tcggggtaat ggtgatcacc acgtaaccat tgccgaccag cgttttcagg
     9661 tcggcatttt ctggaatttc gccctgcacg cgcgccacac cgcgcatttg ctggttattg
     9721 ttaccgttaa taaccgccag attcatcgga ccgtcgcctt gcagctgtac ggtgatatca
     9781 ccatcaaact tcagcgtagc ggttaacagg ctggtcgcaa ccagcagttc tgccagcacg
     9841 tttttaacgg gctgcggata atcgtgattc tcaaggatct gttgcagggt ttccgaaacg
     9901 gttaccagtt cgccgcgcac ggcaaagttt tcaaacagat agcgatgtaa ttggtcatgt
     9961 tgcggcataa tcatctctct tgcaggtgac agttattcac tgtcgccgtg tttaaatcgt
    10021 aacaggtcgc ggcgctcttt tttgtccggg cgtcggtccg ggtgcggcat ggttaaggca
    10081 ttaagtttac gtgccagcgc cattttttcg cgtttctcta cactttccgc agtctcttca
    10141 tacagcaagg ctgcctcgct ggcggggcga cgctgttcag taatcgcctt tacaatcacc
    10201 gtgcgttcgt catttccctg gcgcagagtg agcgtggcat tcagctcgac gattttgctc
    10261 ggcttgctgc gctgcccgtt gtaatgcacc ttaccgcctt caatcatttc acgggccagc
    10321 gcgcgggttt tataaaaacg ggcagcccat agccatttat ccagtcgaac ctcaacagca
    10381 ggtttttctt tcatggcgtc tccttcacat tagcgagggg atcaggcggc ggtagtcatt
    10441 cagtgacgga tggcgttgat actgtttctc ggcaatcccg gaatcaggat tagtcacgcc
    10501 gaggcagtaa cgaataccaa attgcgcggc agcatcgaga atcgcttcgc tgtcatcaat
    10561 aaacagcgtt ctttcagctt tcagacccgt agcttcggcc accgcatgcc ataaccgctg
    10621 atcctctttc ggataaccaa atgtgtgggt ggaaagtaat aaatcaaggt gtgcgtccag
    10681 accggtatgc tcaagtttta ccgccaggtt gtgcggatgc gcattggtga gcaaaattcg
    10741 ctgcttaccg ctggctttca gtgcctcaag aaacggaatg gtatcttcac gcagtacggc
    10801 acgcggtccc atctcggtgg tcatcgcaca gatatccaga cccagttgct cactccagta
    10861 atcaagacag taccagttta gcgtatgctg tacgtcgtga tattgctggc gcatatattc
    10921 catcgcttcc tgtggcgtaa ccccgttttt cgcgccccat gtttcaggca ccagcttttg
    10981 ccagaaatag ttatcgaagg cgaggtcgag caacgtgccg tccatatcca gcagaacggt
    11041 atctacgtcc tgccaggcaa tgttgatatg catgagggaa atctccagag tgaagcaatt
    11101 tgcgcgacag ggtagcataa cctgccgcgc aaacgtgtta ttcgataagg ctttctgaag
    11161 gggtgatcag ttgcgggttc aggcagcttt cataataggc ctgaatcttc atcatgcgag
    11221 tgcgatgacg ctggtaacga cgccaggcct gtacgccatt gtaaatggcg ctacccagca
    11281 tcgtcagtag cagcaatgtg gtgctgagat agcgccacag gccggaacga tccgggattg
    11341 gatgaaggcc aatatgctgc gtaccattgg cgtcagtgaa gatttttgtg acgataccct
    11401 cggcgttaaa cggcgtatgc atcagcattt gtgccagttt ctggaaagcg ttccactgtt
    11461 cttgcggcgg gtagtcgtaa agtgatgccg aaggccaggg ctgatcaaca aaatcgctgc
    11521 cttcgtcgct gacaatcagg aatccgccgg gggccggact gtttagtgat tgtgccgctc
    11581 gcgccgtttc atgcgtgata aacggcgcgg tggaggttgc caccaggtta tccagcgatt
    11641 ccgcactcac cgggcgtaat aacacattca cgccatcgag cttcccggcg ttggcgcgtt
    11701 ttaccagcgc gtcccagtct ttactgttgc cgagattgac cagcgcattt ttcagtcgca
    11761 cacagtcatc tttggcagaa cataaatccg ctgtcttcag tacaatgtcg ccaaaatcat
    11821 caagcaatac catgccggat ttttgaattg ctgaacgtaa tgaggcactg acgcgagatc
    11881 catcttccgg tttagggtgc agctggcgat taactgcttc agtcaatgcc gtcgctttgt
    11941 tgaccagttc agattctggt aatggcaatg agcgggcgtc gttccagatg atctgcgagc
    12001 agtcaaacgg taaaaaaggt gaattggttt tcgcgctcca ggttccggaa gtgcgaatat
    12061 tacacattcc cgtaccgctg atacgcaatg tatcgcctac ccgcacgcca gcgtcagcca
    12121 gttgttttac gctggtggct tcaatggtct gtgcgccttt catccatgag agagtgaatt
    12181 tcagcggcat atccagcggg atccagaata acagcataaa cagcaccagc agcgagcccg
    12241 cagcgataat cgtactgcgc agccagtgct gtaacggaaa gttttttact tcatcatgca
    12301 gcgaaagata tcgcccctga cgcactacgt ggcggtcgag atagatatca atatcggttt
    12361 gttgaccgag atcctgagca atgtatggct gccagtgtgc gggatagacc aggtcgataa
    12421 taccgagtga aatattgttg atctgttcct gatcgttttc gccaaacaat ccccaacgac
    12481 ggggtgtacc gcgcagacaa tgaatttccc gcagggagga ttttgccggg ggcgcgaata
    12541 gcccccacag acctgcgccc agcagcagta atgcgccgcc tgccagccac ggaacaaata
    12601 catccggggt aatcaggcag aaaaagaaca tcaggaagga ggcgacgatc agcaacgctt
    12661 cacgcagccc gcgcggacga ctcagcgcat attcttcatg cgtttctttg cgaatattga
    12721 gtagctcgat ttgctcactc tcttccccgc gaatcgacgc ctgcgtggac ggaacaggtt
    12781 gcaaggcata gccgcgcgtt tcctgcatgt actcctgcag cgtatgaccg ttgagtgaaa
    12841 taaccagcgg cagcgaatcg gtataaatca gttcaacggt attctcatcg ttgatgtatt
    12901 gttcccagaa agggggcagg tggacttcta ccgaatcgag gtagtaacgc catttattcg
    12961 gatcgtcggt agagatgccg taacgcgtga tagcgtgtgt cagcatcatg acgttgttgc
    13021 tttcggcatt cagcgccaga gagatcggtg ccgcgctagc tcccgttggg ccaggcacct
    13081 gtaatacctg cgtcaggctc tcaagataat tttcaacggc gctacgttct tcaggtgtga
    13141 gtttacgcgt ttgcgcatcc gcgaaggcgt tggtccaggg cagctgacgc cgtctggatc
    13201 gcacttttat cagccatccc gcaagtagtg agcaggccag caaagcagct aaaaaaatca
    13261 caatggtgct catgctttcc ccatcttact tagtctgtca ggcgtggtca gtcgcaccag
    13321 acacaataca tgattctgcg gttggctatc ggcaagcgtg gagtttaact tgagcatcat
    13381 taagcacatc acagtaaccg ctgatcatag caaaccctct gaaggcggga tatcagcaaa
    13441 ttcctggagt tatttcaacc tattgacttt taatttgatt tgagga
//
LOCUS       DAZOYL010000094        13465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZOYL010000094 DAZOYL010000000
VERSION     DAZOYL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.094"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1726
                     /gene="adeD"
                     /locus_tag="ACHUOY_001996"
     CDS             <1..1726
                     /gene="adeD"
                     /locus_tag="ACHUOY_001996"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR0682780.1"
                     /translation="AEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRY
                     IAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVI
                     CDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAW
                     RDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIE
                     NCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWE
                     IAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSD
                     ARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGK
                     RYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNE
                     GALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLM
                     STDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDVEKFA
                     FTTLEVTE"
     gene            complement(1771..3162)
                     /gene="uhpT"
                     /locus_tag="ACHUOY_001997"
     CDS             complement(1771..3162)
                     /gene="uhpT"
                     /locus_tag="ACHUOY_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR0682781.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3300..4619)
                     /gene="uhpC"
                     /locus_tag="ACHUOY_001998"
     CDS             complement(3300..4619)
                     /gene="uhpC"
                     /locus_tag="ACHUOY_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR0682782.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4629..6131)
                     /gene="uhpB"
                     /locus_tag="ACHUOY_001999"
     CDS             complement(4629..6131)
                     /gene="uhpB"
                     /locus_tag="ACHUOY_001999"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR0682783.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6131..6721)
                     /gene="uhpA"
                     /locus_tag="ACHUOY_002000"
     CDS             complement(6131..6721)
                     /gene="uhpA"
                     /locus_tag="ACHUOY_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR0682784.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6796..7086)
                     /gene="ilvN"
                     /locus_tag="ACHUOY_002001"
     CDS             complement(6796..7086)
                     /gene="ilvN"
                     /locus_tag="ACHUOY_002001"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0682785.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7090..8778)
                     /gene="ilvB"
                     /locus_tag="ACHUOY_002002"
     CDS             complement(7090..8778)
                     /gene="ilvB"
                     /locus_tag="ACHUOY_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR0682786.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8884..8982)
                     /gene="ivbL"
                     /locus_tag="ACHUOY_002003"
     CDS             complement(8884..8982)
                     /gene="ivbL"
                     /locus_tag="ACHUOY_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR0682787.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9547..9636
                     /gene="tisB"
                     /locus_tag="ACHUOY_002004"
     CDS             9547..9636
                     /gene="tisB"
                     /locus_tag="ACHUOY_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR0682788.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9760..9834)
                     /gene="ysdE"
                     /locus_tag="ACHUOY_002005"
     CDS             complement(9760..9834)
                     /gene="ysdE"
                     /locus_tag="ACHUOY_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR0682789.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9916..11100
                     /gene="emrD"
                     /locus_tag="ACHUOY_002006"
     CDS             9916..11100
                     /gene="emrD"
                     /locus_tag="ACHUOY_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR0682790.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11108..11605)
                     /locus_tag="ACHUOY_002007"
     CDS             complement(11108..11605)
                     /locus_tag="ACHUOY_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR0682791.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11602..11964)
                     /locus_tag="ACHUOY_002008"
     CDS             complement(11602..11964)
                     /locus_tag="ACHUOY_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR0682792.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11954..12301)
                     /locus_tag="ACHUOY_002009"
     CDS             complement(11954..12301)
                     /locus_tag="ACHUOY_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR0682793.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12410..12859
                     /gene="yidI"
                     /locus_tag="ACHUOY_002010"
     CDS             12410..12859
                     /gene="yidI"
                     /locus_tag="ACHUOY_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR0682794.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12906..13064)
                     /locus_tag="ACHUOY_002011"
                     /pseudo
     CDS             complement(12906..13064)
                     /locus_tag="ACHUOY_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13108..>13465)
                     /locus_tag="ACHUOY_002012"
     CDS             complement(13108..>13465)
                     /locus_tag="ACHUOY_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0682795.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3165 a         3767 c         3530 g         3003 t
ORIGIN      
        1 ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
       61 tgataatgtc tctattctcg acctgatcaa tggcggagaa atttccggcc caattgtgat
      121 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatgctccgg ctttgcagcg
      181 gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
      241 atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac
      301 cgttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt
      361 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtacc
      421 cgccctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
      481 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattag
      541 cgggcagaat gcgctgctcg ataaactgga tgcatttcgc cacctgacgc tggacggtca
      601 ctgcccgggt ttggggggta aagaacttaa cgcctatatt actgcgggta ttgaaaactg
      661 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
      721 gatccgcgaa gggtccgctg cccgcaatct caacgcgctg gcaccgttga tcaacgaatt
      781 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
      841 aggacacatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
      901 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
      961 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     1021 gcagcaggta ctggtgaaag gcgagccgat tgatgcgcaa accttacagg cggaagagtc
     1081 ggcgagactg gcacaatccg ctccgccata tggcaacacc attgcccgcc agccagtttc
     1141 cgccagcgac tttgccctgc aatttacgcc cggaaaacgc tatcgggtca ttgacgtcat
     1201 ccataacgaa ttgattacgc actcccactc cagcgtctac agcgaaaatg gttttgatcg
     1261 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcttg
     1321 tggtttgctt ggcggctttg gactgaatga aggtgcgctg gctgcgacgg tcagccatga
     1381 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     1441 ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaggtcc aaagtcatct
     1501 gccgttgccc attgccggcc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat
     1561 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     1621 gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt
     1681 tgatgtcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa aaagcccggc
     1741 gtcatgccgg gcaaaagtca ccagttacgt ttatgccact gtcaactgct gaattttttt
     1801 ctcgcggcgg attttgcgtt cttccatcac cgccactatc gccatcaggc agatacaacc
     1861 aatcgcggcg atatcaagtg cggcgaaggt gcctgcccag ccggtaaggc cgaataccgg
     1921 cgtcccatcg gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa tcaggtaagc
     1981 aaaggtgcct ttaataccat cggcagcgcc aatcgctttt ttaggtacaa agccaacagc
     2041 agccacacca atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa agagagaagc
     2101 cagatagata tattcgttac tggcatgttg atacacaccg agcgtggcga taatcagcgc
     2161 cagcgcgatg caggccacca ggccacggcg accgttcgcc aggtcagaga gccagcccca
     2221 cagcagcgta ccgaccagcg caccagcttc aaacagcgta aagccctgaa tcgccaccgc
     2281 tttagagagt ttcagttcct ggaacgcgta tacggttgac cactggtcga taccaatacg
     2341 taccacatag aggaaaatgt tggcgaagca cagcagccag atcactttgt ttttcagcac
     2401 atactcaaca aagatctgcc acttggtcat atcggtagat tctgtctctt tgtcctcttc
     2461 gctgatctcc tcgccgaaca gttcttcagc tttgccgagg ccataagatt ccggggagtc
     2521 gctgccgtaa cgcaggccga taaaaccgac aatcagcgcg ataatcgacg ggaagataaa
     2581 catgccgatg acatggccat cgaacaggta atttgccccg aacagcgcca cgcctgctgc
     2641 gcctgcaccg ccaaggttgt gagaaatatt ccagaaaccg aggaatgtcc cgcgtttacg
     2701 acgcggcgtc catttggtga tggtggagta actgcacgaa ccgccggtac tctggaaaaa
     2761 gccgcttaag gcgtagaagg caatcatcag gaacaggcta accgagccgc tgcccatact
     2821 ggcactgaag cccagcatac aaatggcaga caggatcagc atgaacggca ggaattgttt
     2881 ggtgtttttg ccgtcggcgt agtaggaaac cagcgtttta cccacgccat aagtgatgga
     2941 gaaacccagg ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg agatcatgtc
     3001 gttctgcgcg atgttaaagt tcttgcgaat caggtacatc gtcaggtagc cgataaagac
     3061 caccaggtag gattgcatga acggtttgaa ccacattttg cgccgcactt cgagcggaag
     3121 gtccagggtc ggcttgcgaa cctggtttaa gaaagccagc atgggttact cctgaaatga
     3181 atacctgcgt gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc
     3241 aggcaaaacc tggaaaattt cttagttttg ccggatatga agtgaaaagg tgagatgcat
     3301 cacgcttcgc gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaatcccg
     3361 gcggcgatag agataaccac aaaaaatccg ctccagtgcc aggtatcgag tactttcgcc
     3421 agcggccaac cagcaagcga cgcccccaga taagcaaaca agccgacaaa ccccgtcgcc
     3481 gcccctgccg cctctttgtg ggaacactct gccgccgcca taccgattaa catctgtggg
     3541 ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat
     3601 ggcatcagcc acagggagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc
     3661 ggccctcggt tgccgttaaa caatttgtcc gagccccaac ccgctaccag cgcaccgata
     3721 aatccaccca gttcaaacat cgtcactgcc gtattcgccg tgaccagatc gacgcccagc
     3781 gtctcggaca tatacaaatt gccccagtcg ttgatcgccg cccggaccac atagaccagc
     3841 acatagcaaa acgaaagcag ccagatatac ggattcagca acacatattt ggtgaggatc
     3901 tctttacgcg tcagccctgc cccttcttgt tgttgagcta tctccagcgc gtcgtgtcgc
     3961 cattcaccga ccgccggtaa acctaacgcc tgcgggcgat cgcgcagccg ccagcagaga
     4021 aaaattccca cgacgatcgc catacaacca gcaatcatca tcccggcacg ccagccgtaa
     4081 tgtagtgcag ccgctgccat cacaatggga atgagtgcgc cgccgacgtt atgcgccgtg
     4141 ttccataatg cccaccaacc accgcgctcg gtacgtgaat accaggccgt taacagacgc
     4201 gcacacaccg gtgaacccca gccctggaaa aaggcgttca gcacccagag tacggcaaac
     4261 gcccatagcg acgtcgagaa gccaaacaga atgttgataa tgcccgtggc gataagccct
     4321 atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac
     4381 acgccatagg taatgtaaaa cagggtcgct aacaggccga tatcgctacg gctgagcacg
     4441 ccgttagcaa ggatttctgg tacggcggcg ttaaaacttt tccgcgtgaa gtaaaacagc
     4501 gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata gcgggcatca
     4561 atttcatatt tatcagccat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc
     4621 atcaaacctt agacatagcg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag
     4681 gaaatgtgta atgtgccacc cagcgccgtt acgcgctcgc gcattccggt gaggccaaaa
     4741 ccttgttgcc cggaacccgg cggcaaaccg ctgccatcgt cttcaataac cagcatcaac
     4801 cgttcatcct gctgccagcc ttgcagggtg accgcgctgg catcagcatg tttcacaatg
     4861 ttgttcagcc cttcctggca gacacgaaac agcgtcacgc gctggttttc gcttaacgct
     4921 gattcatcga ttcgccattc gagatggctg acaataccgc gcccttccag ctccatttcc
     4981 cgcatcagtg agcggatggc ctgctccagg gtgagatcat ccaactggcg cggacgtaac
     5041 cgacccaaca aacggcgcac cgcgtcgtaa acgcccagcg atagttgttc gatgagttgc
     5101 ccgctctgct tcacgctggc gttatctgcc gccagccgct gaacaatgcc cgcctgagta
     5161 cgaatagcag tgatggtctg gccgatatca tcatgcagct cacgcgccac atcacggcgc
     5221 acgctctctt cggtttccag caaccgttca gccagatgct gattgcgcgc cagttccttt
     5281 tgcagcgact ggttaagttc acgcaaccgc tggatgccag cgccaagcaa caaccctgtc
     5341 agactttgca ccagcagcga gagcaataaa tccaccggat gatcgcgcca ggtttgactg
     5401 gcgatcagcg cgatggcgtt catcaacgtc gcaatcagcg ccccttgcca accatagtgc
     5461 caggccagcg cgataatcgg cagcaccaga cagaatggcg taaagcgcga cagttcgtcc
     5521 ggcaatccca actggagcca gagactgata acaaacagca gcaagtacca gaccagatgt
     5581 cgcccgcgcc agttgattgg ctgagaaacc agtgacgggc cgagcggcag ccaggtgtta
     5641 ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcaggcc gccagttaaa
     5701 gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac
     5761 aacgccgccg ccgttaacgc cgccccctgt aacagcaagg tgcgccagtc acgctgatgg
     5821 cgatagcgcg agatcagcgc tacgggcagt aacgtcagta aactaccgat catcaataac
     5881 ggaaaatggg ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca ctccgcaccc
     5941 agcaatacgg gccagtatcc gcgcgggcat tgcagcatta gccccagacg cagaccaaac
     6001 ggaaataaca gcaccgccat atcagggcgc tcaaccagat gcaggctgat actccacagg
     6061 caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac
     6121 aacgtcttca tcaccagcca tcaaacatgc ggcgcgccag ctctacgtcg ttactgacgc
     6181 ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggtgac aagcccagtt
     6241 cggcggcaat ctctttcacc gccattcctt gcgccagttt ttccgccacc tggcgttcac
     6301 gtttggttag cgggtcctga cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat
     6361 aacagccacc cgtggcaacc gtatgcaccg cagcaatgag ttcatccggg ctacagcgtt
     6421 tggaaagaaa gccgcgtgcc cccgcgttaa gcgcctgctc aaccagcgca ggactgtcgt
     6481 gaacggagag cataatcgtc gccatacctt tcggcagctg gcttagcagc tccagaccgg
     6541 agatatcggg catggagata tcgcaaatac acacctgcac accgcgcccc ggcagccccg
     6601 ccagcgcctc gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca
     6661 gcagctgcgc aaagccggag cggacgatga ggtgatcgtc tataagggca acggtgatca
     6721 tggtcttgtc ctggcgggta aaaaaaaacg cgcttacctt aacgataagc gcgatgttgt
     6781 tcaagccttg agcggttact gaaaaaacac cgcgatcttg ttaaacatcg tcggatcgga
     6841 ctgattacgc tgcactttca cgacatcttc cagcttatcg atttggctta tcatctgctc
     6901 cagacgctgg tcgtcattga ccagtagcca gatatggctt ttgtcgctgt cctgaatcgg
     6961 cagacaaaga atgccttcaa cgttaaaagc gcggcgggca aaaaggccac aaacgtgggt
     7021 cattacgccc ggatggttgc gaacggtgag ctccagaatt acgttgtcat gagttgtgtt
     7081 ttgcatggct tattccccca ccatttcagt attcgccgca cctggcggca ccatcgggta
     7141 aactttttct tcggcatcaa tgcgcacatg gatcagcgcc gggctagggc gattgatgat
     7201 ttcctgcaat gcagcctgcg gatcggcttc gttattcaaa tcacaggttt cgaggccgaa
     7261 tccggcggca atctgcataa agttgatttt gcccggatag gtggcggcaa aaacgccttg
     7321 ctcgtagaac agactctgtt gctgatgcac cagccccagc gcttcgttgt tcatcagaat
     7381 gattttgaca tccagctgat tttcactggc ggtcgccatc tcctgaatat tcatcatcag
     7441 gctgccgtcg ccggagaaac acaacacttt gcgatccggg ttcgccagcg ccgcgccaat
     7501 cgccgcaggc aggccaaaac ccatcgtgcc cagcccaccg gaggtcagcc actggcgtgg
     7561 gcgattgagc ggataagctt gcgcggtcca catctgatgc tgacccacat cggtggtgat
     7621 aatcgcattg tcatcgacac aggcggcaac ggcgttgatc aggccgtaat ggcttaatgg
     7681 atcgcacgct ttcgggattg gacacgggaa ttcgcgctgc aaatccgcta ccaactggtg
     7741 ccactctgca cgcggttgcg cttccacctg cgggatcaat tgcgccagca cgtcatcaac
     7801 atccgcctga atcgccacat gcggctgctt gattttaccc agctctgcgc gatcgatatc
     7861 gacatgaata attttggcat tcggacagaa ctgctcggtt ttgccaatcg cccggtcatc
     7921 aaaacgcgca ccgagcacaa tcaacaaatc cgcctcctgc aaaatatagt tggtactgcg
     7981 cacgccgtgc atccccagca tacccagcga caacggatgc gcttttggca gcatgcccag
     8041 cgccattaaa gtcatggtgg taggcagttg cgctttctcc gccagttcac gcacccgcgc
     8101 aggcgcattg atcacaccac cacccagata aagcaccggg cgtttggcag cgttaatcat
     8161 tgtagctacg tcacgaatgc tttcttcgct aaaggcgggt gcagcggctt tttctgccac
     8221 cgcgggctga gcttcaatct caaaaaccgc cgtttgtaca tccttaggaa tgtctatcca
     8281 caccgggcca gggcggcctg attgcgcaat gcggaaggca tcgctcatga cctgcgggag
     8341 ttcttcgata tgtctgacca gatagttgtg tttggtgatg gggatagaga tgccgtaggt
     8401 gtcgacttcc tggaaggcgt cggtgccgat catcgaggcg ggaacctgac cagtgatgca
     8461 aatcagcggg atggagtcca gccgcgcatc ggcaatggcg gtcaccaggt tagtcgcacc
     8521 cggtccgcta caggccatac agaccgccgg tttaccgtcg gtgcgcgcca ttccctgagc
     8581 gataaagccc gcgccctgtt catgacgggc cagaatatgg cggatttgcg tgctttggct
     8641 taaggcatcg taaacaggca ggatagaacc gcccggaatg cctgtcacaa tcttaatgcc
     8701 ctgctgttcc aggaaatgaa cgataaattc tgcgccggta aagcgcttac gcgtcaatgt
     8761 tgtgcccgaa cttgccatgc tccagtcctt ttcttctggg ccgactttcc gggaggtgct
     8821 taaacgaaaa accccgcccg gtttgcgccg acggggtttt ggaatcgtgt gttgttccag
     8881 tccctacggc gcattgccga cgaccaccac cacacgcacg acgaccactg cggcggatgg
     8941 cgcagttggt agtagttttg cgttgaacat ggaagtagtc attggggacc ttgttggttt
     9001 tgtgtttaac aatatttata caagcacagc tttacagggg agacaatgga aaatttttca
     9061 gcaagggaaa attgaggggt tgatcacgtt ttgtactgaa ttgcagataa caaaaaaccc
     9121 cgccggagcg aggtttcgtc agtcgcccgc ggctggtaac cgcaaagcac actgtattat
     9181 gtcaacacag aaagtatacg tgttccgcgc agaacgcgca atttcggcac gaattttgac
     9241 gtatttagtg catagttgag tatcgatcac agtttgcgtt ttgtccaaat attgctgttt
     9301 atttatacag taaacttcta taatatcact gtacgcaatg tgttatgcgg gggccgcatc
     9361 gttacccggc gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc ttaaccccgc
     9421 gtgagcacac tgtgttatgt caacaagcac aaagtttctc cttgagatac cgcgtgcaca
     9481 acagctggca acaggcagcg gaaaggtacg tcagctggca gtgctcctga accacaggag
     9541 acgcgtatga gcctggtgga tatcgccatt cttatcctca aactcattgt tgcagcactg
     9601 caactgcttg atgctgttct gaaatacctg aagtaattca gattcaagtc gcaccaaagg
     9661 ggagcgggta accgctcccc ttttatattt agcgtgcggg ttggtgtcgg atgcgatgct
     9721 gacgtatctt atccgcccta ccatctctcc cggcaacatt tattgccgct tttgtttaca
     9781 tattctgccg ctaaacaact ccccattcct ggcgtatatc tggctaacat tcatcaatgt
     9841 gatagattcc tctcccgcat ttatgggaat gcgtagtgac ttactctaat tatttttata
     9901 aaagcatccg tgataatgaa aaggcataga aacgtcaatt tgttattgat gttggtatta
     9961 ctcgtggccg tcggtcagat ggcgcaaacc atttatattc cagctattgc cgatatggcg
    10021 cgcgatctca acgtccgtga aggggcggtg cagagcgtaa tgggcgctta tctgctgact
    10081 tacggtgtct cacagctgtt ttatggcccg atttccgacc gtgtgggtcg ccgaccggtg
    10141 atcctcgtcg gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac gacctccagt
    10201 ttgacggtat tgattgccgc cagcgcgatg caggggatgg gcaccggcgt tggcggcgta
    10261 atggcgcgta ctttgccgcg agatttatat gaacggacac agttgcgcca tgctaacagc
    10321 ctgttaaaca tggggattct cgtcagtccg ttgctcgcac cgctaatcgg cggtctgctg
    10381 gatacgatgt ggaactggcg cgcctgttat ctctttttgt tggttctttg tgctggtgtg
    10441 accttcagta tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc gcgcacgcgc
    10501 ctgcttacca gttataaaac gcttttcggt aacagcggtt ttaactgtta tttgctgatg
    10561 ctgattggcg gtctggccgg gattgccgcc tttgaagcct gctccggcgt gctgatgggc
    10621 gcggtgttag ggctgagcag tatgacggtc agtattttgt ttattctgcc gattccagca
    10681 gcgttttttg gcgcatggtt tgccggacgt cccaataaac gcttctccac gttaatgtgg
    10741 cagtcggtta tctgctgcct gctggctggc ttgctgatgt ggatccccga ctggtttggc
    10801 gtgatgaatg tctggacgct gctcgttccc gccgcgctgt tctttttcgg tgccgggatg
    10861 ctgtttcctc tggcgaccag cggcgcgatg gagccgttcc ccttcctggc gggcacggct
    10921 ggcgcgctgg tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc gctctctgcg
    10981 atgttgccgc aaaccggtca gggcagccta gggttgttga tgaccttaat gggattgttg
    11041 atcgtgctgt gctggctacc gctggcgacg cggatgtcgc atcaggggca gcccgtttaa
    11101 gcgcacgtca ccgcagcatc gtcatcagct ccatgggaga acgatgctgc tttatcagat
    11161 cacgcatcac ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag cccgcacaaa
    11221 gcacgctgtc tggcgtctcc tgccgagcgt gcgggcacag gcgatgccag tcgcacgcct
    11281 ggcagtcaca gcgcggttgc gggttttcgc tgccacacca tttttgtaag gtggcgtcaa
    11341 aaagctcaat cgagatacga ctgatatctg cccgcaccca acactcgaac agggcaatta
    11401 aaaatttccc ccacagctct gctgtcaccg aagcggccgt cagatcgccc cggtcatcaa
    11461 attcaagata gggaatgagc tgtacggagc ggatgccccg ctgtttgaaa ttgtgataaa
    11521 aatcggcagg ttgcagagca tccttgtact ctacaaccag tttatgcctg tcctcttcag
    11581 catcaatgac ctgacttcct gtcatgcgac tctctcaata aatacgataa gttgatgtat
    11641 atgcgttaca gcaaacagta ttgcgagaga taacaccgcg agggagatca aaaatttgtc
    11701 acggactacg tgaaattggg aaaaatcgct attcgtgaca tccattaaat tgcgattacg
    11761 ggtgtagtgc cagaggatca gcgccacgat ggcgagaatg ccaatcgaaa tccagaataa
    11821 catgcccgcc tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc cgtagcccag
    11881 catggtacga aaccacgcca gtgatgtgcg ctccggctgc agtcctgggt cggcaatgcg
    11941 tctggctttg cggctatccg gcatacaaca ccagtcccat aacgatcacc gccacgacca
    12001 tcaatattaa gctaatgatt aataagctgt tggtgtaggg caggtcctct ttcagacgca
    12061 tcgctttttc attgcgtaac cagcgcaggt agccatacat tgctaacccg ccggaaaaca
    12121 gacacaacaa caacgccagc agttcacgaa tgacgggcgt ggcgaaatct ggcgcaagtt
    12181 gatcaagccc aacccccgcc gccagaaatc ctagtgcggt acggatccac gccagaaaag
    12241 tacgctcatt agccagcgag aagcggtaat cgggcgcttc tccaaggcgg gaaattttca
    12301 ttgttgctcc tttgcaattt cactaactct ctacctttag cggttttttt taagaatttc
    12361 gtccgcccgt ttcctgatat gcttttgcca tccgctacaa ggaggtcaga tgggtatcat
    12421 agcgcaaaat aaaatcagtt cattgggcat gctgttcggt gccatcgctt taatgatggg
    12481 gattatccac ttttcattcg gccctttttc cgcaccaccg ccaacgcttg aaagcatcgt
    12541 ggcggacaaa acggctgaaa ttaaaagagg tcttctcgcc ggaataaaag gcgaaaaaat
    12601 taccaccgtg gagaaaaaag aggacgtaga tgtcgataaa atcctcgatc aaagcggtat
    12661 cgcactggcc attgctgcgc tactttgcgc atttgttggc ggtatgcgta aggaaaatcg
    12721 ctggggcatt cgcggcgcgc tggtttttgg cggcggaacg ctggctttcc ataccttgct
    12781 ttttggtatc ggcatcgtgt gcagcatttt gctgatattc cttatctttt ccttcctgac
    12841 cggcggttcg ctggtttaag cagcctgaat aatgcccgcc gggtgatcat cagcgggcat
    12901 tatcgtcaga acttctgttt tttctcttcc accttcaccc cttgtgtcgg taggccggtg
    12961 tcatagtcac gtaccaccgg cgaatagcca tcttgtgggc gtggacgaaa tgcccccatc
    13021 cagcgcggcc gtgcatcttt acgccacgga cgcaggctcc attgttgaca tcctccacgc
    13081 cctgaaggac gtggacccct gctacgttca ggctgtcgcc tgaatcattt cggtgggttc
    13141 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    13201 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    13261 tctgaattta ctttgtgaca ggcgattttc tttcgctgta tgaccacagc acacgcaacg
    13321 ctggcttgtg tacgctggcg gaacagcaag cacctgacca ccacgccaga gctgcttgta
    13381 cgcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    13441 ttttgcccgg acattgcgcc ccggc
//
LOCUS       DAZOYL010000095        13260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZOYL010000095 DAZOYL010000000
VERSION     DAZOYL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.095"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            111..2897
                     /gene="polA"
                     /locus_tag="ACHUOY_002013"
     CDS             111..2897
                     /gene="polA"
                     /locus_tag="ACHUOY_002013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR0682796.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(3279..3911)
                     /gene="yihA"
                     /locus_tag="ACHUOY_002014"
     CDS             complement(3279..3911)
                     /gene="yihA"
                     /locus_tag="ACHUOY_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR0682797.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            4208..4374
                     /locus_tag="ACHUOY_002015"
                     /pseudo
     CDS             4208..4374
                     /locus_tag="ACHUOY_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4493..5002
                     /gene="yihI"
                     /locus_tag="ACHUOY_002016"
     CDS             4493..5002
                     /gene="yihI"
                     /locus_tag="ACHUOY_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR0682798.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            5191..6564
                     /gene="hemN"
                     /locus_tag="ACHUOY_002017"
     CDS             5191..6564
                     /gene="hemN"
                     /locus_tag="ACHUOY_002017"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR0682799.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(6754..6864)
                     /locus_tag="ACHUOY_002018"
     CDS             complement(6754..6864)
                     /locus_tag="ACHUOY_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR0682800.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6976..8385)
                     /gene="glnG"
                     /locus_tag="ACHUOY_002019"
     CDS             complement(6976..8385)
                     /gene="glnG"
                     /locus_tag="ACHUOY_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR0682801.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(8397..9446)
                     /gene="glnL"
                     /locus_tag="ACHUOY_002020"
     CDS             complement(8397..9446)
                     /gene="glnL"
                     /locus_tag="ACHUOY_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR0682802.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(9620..11029)
                     /gene="glnA"
                     /locus_tag="ACHUOY_002021"
     CDS             complement(9620..11029)
                     /gene="glnA"
                     /locus_tag="ACHUOY_002021"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR0682803.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            11402..13225
                     /gene="typA"
                     /locus_tag="ACHUOY_002022"
     CDS             11402..13225
                     /gene="typA"
                     /locus_tag="ACHUOY_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR0682804.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3322 a         3382 c         3497 g         3059 t
ORIGIN      
        1 gcgatccaga agatctacaa aagattttca cgaaaagcgg tgaaaaactc atgttttcat
       61 cctgtctgtg gcatccttta cccataatct gataaacagg cacggacatt atggttcaga
      121 tcccccaaaa tccacttatc cttgtagatg gttcatctta tctttatcgc gcatatcacg
      181 cgtttccccc gctgactaac agcgcaggcg agccgaccgg tgcgatgtat ggtgtcctca
      241 acatgctgcg cagtctgatc atgcaatata aaccgacgca tgcagcggtg gtctttgacg
      301 ccaagggaaa aacctttcgt gatgaactgt ttgaacatta caaatcacat cgcccgccaa
      361 tgccggacga tctgcgagca caaatcgaac ccctgcacgc gatggttaaa gcgatgggac
      421 tgccgctgct ggcggtttct ggcgtagaag cggacgacgt tatcggtact ctggcgcgcg
      481 aagccgaaaa agccgggcgt ccggtgctga tcagcactgg cgataaagat atggcgcagc
      541 tggtgacgcc aaatattacg cttatcaaca ccatgacgaa taccatcctc ggaccggaag
      601 aggtggtgaa taagtacggc gtgccgccag aactgatcat cgatttcctg gcgctgatgg
      661 gtgactcctc cgataacatt cctggcgtac cgggcgtcgg tgaaaaaacc gcgcaggcat
      721 tgctgcaagg tcttggcggg ctggatacgc tgtatgccga gccagaaaaa attgctgggt
      781 tgagcttccg tggcgcgaaa acaatggcag cgaagctcga gcaaaacaaa gaagttgctt
      841 atctctcata ccagctggcg acgattaaaa ccgacgttga actggagctg acctgtgaac
      901 aactggaagt gcagcaaccg gcagcggaag agttgttggg gctgttcaaa aagtatgagt
      961 tcaaacgctg gactgctgat gtcgaagcgg gcaaatggtt acaggccaaa ggggcaaaac
     1021 cagccgcgaa gccgcaggaa accagtgttg cagacgaagc gccagaagtg acggcaacgg
     1081 tgatttctta tgacaactac gtcaccatcc ttgatgaaga aacactgaaa gcgtggattg
     1141 cgaagctgga aaaagcgccg gtatttgcat ttgataccga aaccgacagc cttgataaca
     1201 tctctgctaa cctggtcggg ctttcttttg ctatcgagcc aggcgtagcg gcatatattc
     1261 cggttgctca tgattatctt gatgcgcccg atcaaatctc tcgcgagcgt gcactcgagt
     1321 tgctaaaacc gctgctggaa gatgaaaagg cgctgaaggt cgggcaaaac ctgaaatacg
     1381 atcgcggtat tctggcgaat tacggcattg agctgcgtgg gattgcgttt gataccatgc
     1441 tggagtccta cattctcaat agcgttgccg ggcgtcacga tatggacagc ctcgcggaac
     1501 gttggttgaa gcacaaaact atcacttttg aagagattgc gggtaaaggc aaaaatcaac
     1561 tgacctttaa ccagattgcc ctcgaagagg ccggacgtta cgccgccgaa gatgcggatg
     1621 tcaccttgca gttgcatctg aaaatgtggc cagatctgca aaaacacaaa gggccgttga
     1681 acgtcttcga gaatatcgaa atgccgctgg tgccggtgct ttcacgcatt gaacgtaacg
     1741 gtgtgaagat cgatccgaaa gtgctgcaca atcattctga agagctcacc cttcgtctgg
     1801 ctgagctgga aaagaaagcg catgaaattg caggtgagga atttaacctt tcttccacca
     1861 agcagttaca aaccattctg tttgaaaaac agggcattaa accgctgaag aaaacgccgg
     1921 gtggcgcgcc gtcaacgtcg gaagaggtac tggaagaact ggcgctggac tatccgttgc
     1981 caaaagtgat tctggagtat cgtggtctgg cgaagctgaa atcgacctac accgacaagc
     2041 tgccgctgat gatcaacccg aaaaccgggc gtgtgcatac ctcttatcac caggcagtaa
     2101 ctgcaacggg acgtttatcg tcaaccgatc ctaacctgca aaacattccg gtgcgtaacg
     2161 aagaaggtcg tcgtatccgc caggcgttta ttgcgccaga ggattatgtg attgtctcag
     2221 cggactactc gcagattgaa ctgcgcatta tggcgcatct ttcgcgtgac aaaggcttgc
     2281 tgaccgcatt cgcggaagga aaagatatcc accgggcaac ggcggcagaa gtgtttggtt
     2341 tgccactgga aaccgtcacc agcgagcaac gccgtagcgc gaaagcgatc aactttggtc
     2401 tgatttatgg catgagtgct ttcggtctgg cgcggcaatt gaacattcca cgtaaagaag
     2461 cgcagaagta catggacctt tacttcgaac gctaccctgg cgtgctggag tatatggaac
     2521 gcacccgtgc tcaggcgaaa gagcagggct acgttgaaac gctggacgga cgccgtctgt
     2581 atctgccgga tatcaaatcc agcaatggtg ctcgtcgtgc agcggctgaa cgtgcagcca
     2641 ttaacgcgcc aatgcaggga accgccgccg acattatcaa acgggcgatg attgccgttg
     2701 atgcgtggtt acaggctgag caaccgcgtg tacgtatgat catgcaggta cacgatgaac
     2761 tggtatttga agttcataaa gatgatgttg atgccgtcgc gaagcagatt catcaactga
     2821 tggaaaactg tacccgtctg gatgtgccgt tgctggtgga agtggggagt ggcgaaaact
     2881 gggatcaggc gcactaagat tcgcctgaac atgccctttt ttcgtaagta agcaacataa
     2941 gctgtcacgt tttgtgatgg ctattagaaa ttcctatgca acaactgaaa aaaaattaca
     3001 aaaagtgctt tctgaactga acaaaaaaga gtaaagttag tcgcgtaggg tacagaggta
     3061 agatgttcta tctttcagac cttttacttc acgtaatcgg atttggctga atattttagc
     3121 cgccccagtc agtaatgact ggggcgtttt ttattgggcg aaagaaaaga tccgtaatgc
     3181 ctgatgcgct atgtttatca ggccaacggt agaattgtaa tctattgaat ttacgggccg
     3241 gatacgccac atccggcaca agcattaagg caagaaaatt attcgccgtc ctgcgtttct
     3301 tctacaggct gcatctcgct aaaccaggta tccagtttct gccgcagctt gtccacgcct
     3361 tgtttcttca acgaagaaaa cgtttcaacc tgcacatcac cgttaaacgc cagtacagct
     3421 tcacgcacca tattcaattg cgctttacgt gcgccgcttg ccagtttgtc cgctttggtc
     3481 agcagcacca gaacggcgat attgctgtct accgcccact caatcatctg ctgatccaaa
     3541 tctttcagcg gatggcgaat atccattagc accaccagac cttgcaggct ctgacgtttt
     3601 tcgaggtatt cgccgagcgc acgctgccat ttgcgcttca tctcttccgg gacttccgca
     3661 taaccgtacc caggcaagtc aaccagacgc ttgccgtcag ccacttcaaa caggttgata
     3721 agctgggtgc gccctggggt ttttgaggta cgagccaggc ttttctggtt agtcagcgtg
     3781 ttcagcgcgc tggatttacc tgcgttggaa cggcctgcaa aagccacttc aattccggta
     3841 tcggaaggta ggtggcgaat atcaggcgca ctcatcacaa aatgcgtctg ttgataattc
     3901 aaattagtca aagcggtcgt ctccgtcagt caaagctttg tggcgattat acctgaacca
     3961 taataaaagg cggatttttc ggcgtgagcg ttgtaagtaa aagccatacg ctttgtgaga
     4021 cattgccgat agtctttatg cgaaatagca gagaaaattc tgcgatgcat gtcaaataag
     4081 ctatataaat cagtgaattg cttttatata aaactctgaa aatgtgcaaa tactgatggc
     4141 ggttgattgt ttgtttaaag caaaggcgta aagtagcacc catagagcga ggacgctaac
     4201 aggaacaatg actcaggatg agggtcagga gcgccaggag gcgaagacag aggattgtca
     4261 ggaagacaaa cgtccggaga cgtaattaaa cggaaatgga atcaacacgg attgttccgg
     4321 ctaaaggaaa aacagggtgt gttggcggcc tgcaaggatt gtaagacccg ttaagggtta
     4381 tgagtcagga aaaaaggcga cagagtaatc tgtcgccttt tttctttgct tgctttctgt
     4441 tagattccgc cgcaaatcta tactgaataa aacggctaaa agacgaacca ttatgaaacc
     4501 atcatcttca aactcacgca gcaaaggtca tgcaaaagcg cgtcgaaaaa cacgcgagga
     4561 gctggatcag gaagctcgtg atcgcaagcg tcagaaaaaa cgtcgtggtc atgcgccggg
     4621 cagccgcgca gcgggcggaa acaccacgtc aggcagcaaa ggccagaacg caccaaaaga
     4681 tccacgtatt ggcagtaaaa cccctattcc attgggcgtg gctgaaaaag tcaccaaaca
     4741 gcacaaaccg aagagtgaga aacctatgct ttcaccgcag gcggagttgg aattactgga
     4801 aacggatgag cgtctggatg cgctgctgga acgtctggaa gcaggcgaaa ccctgagcgc
     4861 cgaagagcaa tcctgggtgg atgctaaact ggatcgtatt gatgagttga tgcagaaact
     4921 cggcctctct tatgacgatg acgaagaaga ggaagaagac gagaagcaag aagacatgat
     4981 gcgtctgctg cggggtaact aacggattgc caccgtgggc cttcccgttc tgctgataac
     5041 ccttccggtt atatgttatc tggtgtggtt attcgttaaa ctacagcggt tgtcgcggcg
     5101 acaaaagtgg ctgcgcaacc ggctgatgac ccgaaacggg catccgccgg tacgccgtag
     5161 ccgccagaga cgccatcgga aggagtgagc atgtctgtac agcaaatcga ctgggatctg
     5221 gccctgatcc agaaatataa ctattccggg ccacgataca cctcgtaccc gaccgcgctg
     5281 gagttttcag aagacttcgg cgaacaggcg tttttacaag ccgtggcgcg ctatcctgag
     5341 cgtccattat ctctctacgt acatatcccg ttctgccata agctttgtta cttctgcggt
     5401 tgcaataaga ttgttactcg ccagcagcac aaggccgatc agtatctgga cgcgctggag
     5461 caagaaatcg tccatcgtgc accgctgttc gccgggcgtc acgtcagcca attgcactgg
     5521 ggcggcggaa cgccgacgta tctgaataaa gcgcaaatca gccgcctgat gaagctgctg
     5581 cgcgaaaact tccagttcaa tgccgatgcg gagatttcga tcgaagtcga tccgcgggaa
     5641 atcgaactgg atgtactcga tcatttacgc gccgaaggct ttaatcgcct gagcatgggc
     5701 gtgcaggact tcaacaaaga agtgcaacgt ctggttaacc gcgagcagga tgaagagttc
     5761 atctttgcac tgcttaacca tgcgcgtgag attggtttta cctccaccaa catcgacctg
     5821 atttacggcc tgccgaaaca gacgccggag agtttcgcct ttaccctgaa acgtgtggcg
     5881 gagctgaacc ccgatcgtct gagtgtcttt aactacgcgc atctgccgac catttttgct
     5941 gctcagcgca aaatcaaaga tgctgacctg ccgagtccgc agcaaaaact cgatatcctg
     6001 caggaaacca tcgccttcct gacgcaatcg ggctatcagt ttatcggtat ggatcacttt
     6061 gcccgtccgg atgacgagct ggcggtggcc cagcgtgaag gcgtgctgca tcgtaacttc
     6121 cagggctaca ccactcaggg cgataccgat ctgctgggga tgggcgtttc cgccatcagc
     6181 atgattggcg actgctacgc gcagaaccag aaagagttga agcagtacta tcagcaagtg
     6241 gatgaacaag gcaatgctct gtggcgtggt attgcgctaa cgcgtgatga ctgtattcgc
     6301 cgcgatgtga ttaagtcgct catctgcaac ttccgtctgg attacgcccc tattgagaaa
     6361 cagtgggatt tgcacttcgc tgattacttt gcggaagatc tcaagctgct cgccccgtta
     6421 gcaaaagatg ggctggtgga tgtggatgag aagggaatac aggtgacggc gaaaggtcgc
     6481 ttgctgatcc gcaacatttg catgtgcttt gatacctatc tgcgccagaa agcgcggatg
     6541 cagcagttct cacgggtgat ttaaatagtg aatggcgctt cgtttacaaa gtagaaaacg
     6601 aaagcgccat caatgtgctg agaacaagat tgcctgatgc gctacgctta tcaggcctac
     6661 aaaacctgtt gaaaatattg cttttgtagg ccggataagg cgttcacgcc gcatccggca
     6721 tgaacaaaga gcacttcatc agcaatctga aactcaacta aacagcccaa tcattgcggc
     6781 acacagcacg gcagcaacgg cagtttgcgg tgtgtggctg tcaactgcgc cactcgatac
     6841 cagattaatt attgattcca gcataatgac tctccccgtt ttccgggcaa gatcatactg
     6901 aacttatcgg aacagtaaag cgtaaaatac cagcaatttg cgctcaataa tcaatcttta
     6961 cacacaagct gtgaatcact ccatccccag ctcttttaac ttacgcgtca gggtgttgcg
     7021 gccccagccg agtagccgcg ccgcttcctg tttatgcccc tgcgtatgtc gtaacgcggt
     7081 cgtcagtaac gtccgctcca gctctggctg cgcttcggaa agcagatttt gatgaccgga
     7141 acgcagcgct ctgtctgccc actgcgcaag aagcgttgcc cagctgtccg gttgcatttg
     7201 cgaagtactc tccgcaaccg ttgattcaaa cagttcgccg ggcaaatcct gaatcaacac
     7261 ttcctgcccg gcggccatca ccgttagcca gcggcaggtg ttttccagct ggcgcacgtt
     7321 gcctggccac gccagacgcg tcagagcagc ttcggtttcc ggatgcagta acttcgcttc
     7381 tacgcccagt tcgcgcgcgg caacctgtaa aaaatggcgc gccagacggg gaatatcttc
     7441 ccgacgttcg cgcagcggcg gcagatgaac gcggataacg ttcaggcggt ggaacagatc
     7501 ctcacggaac ttaccttcct gcactcgctg ttcgagattc tggtgagtgg cagcgataat
     7561 acgcacatcc actttcaccg gcgcatagcc gccaacgcgg taaaactgac cgtctgccag
     7621 cacgcgcagc aaacgcgtct gcacatccag cggcatatca ccaatttcgt cgaggaataa
     7681 tgtaccgcca tcggcctgtt caaaacgccc ctgacgaatg gtattcgcgc cagtaaacgc
     7741 gcctttctcg tggccaaaca gttctgattc gatcaaatct tttgggatag ctgccatatt
     7801 cagcgcgata aacggcgctt tggcgcgcgg actgtggcga tgcagggcat gagcgaccag
     7861 ttctttaccg gtgccggatt cgccgttaat cagcacgcta atagaagaac gcgaaagccg
     7921 accgataata cggaacacgt cctgcatggc tggcgcttcg ccgatgatat cggtcgttgg
     7981 gccgttaagc tgaatattac gcggctgctg ctgttcctgg taatgactga tagcgcgctc
     8041 aaccagcgcc actgcttcgt cgatatcaaa cggtttgggc agataatcaa acgccccttg
     8101 ttgataggcg ctgacggcag catccagatc ggaatgtgcg gtcataatga tgaccggaag
     8161 cattggatgg cgctgtttaa tctgcttgag cagcgccagc ccgtccattc ccggcatacg
     8221 gatatctgaa agcagcacat ccggcgtttt gctcgccagc gcctccagta cttccgcgcc
     8281 gttctcaaat gtcgtacagg ttaaacccgc tccagcgagc gcacgttcaa gcacccaacg
     8341 gatggaacta tcgtcatcga ctacccagac tatccctcgt tgcataaacg tcacctttat
     8401 ttcctgatag gcaggtaaac cgagaactcg gtatgacctg gccaactggt aaattcaatt
     8461 ttgcctgaat gctgatcaat caaattacgg gcgatggata agccaagccc ggtgccacct
     8521 tcgcggccgc tgaccatcgg gtaaaacagc gtatcctgca aatgaggcgg aatgcccggc
     8581 ccgttatctt ccacatcaat ccgcgccgcc agccggtagc gctcgccgtg taaggtcagt
     8641 tgaaacgcgg tgcgggtacg cagaatgatt tcaccgcctt ccggccccag cgcctgtagc
     8701 gcattgcgca caatattcag caagacctgt tcaatttgat ccgggtcgtg cgccagttcc
     8761 ggcaggctgg ggtcgtaatc acgaatcaac cgcacgttgt ccggcagttc catcgacacc
     8821 agcgtcacca cgcgttcagc tactttgtga atactttcgg taacgcgcgt accgggcagc
     8881 tgcggcccca acagacggtc gaccagattt cgcagccggt ccgcctgttc gataatcact
     8941 ttggtatatt cgagtagtga tgggtcaggt aacgctttgc tgagcagctg cgccgcgcca
     9001 cgtaaacctc caagcggatt tttaatctca tgtgccaggc cgcgcactaa atcacgggca
     9061 gcaacctgct gggcgtgctg tagctgttcc tgacttaagc ggcgctggtt atccatcgga
     9121 gccatctcca gcaggatcat gccgtccggc atacgctggg ccgtcacaga aaggatatgc
     9181 gagcgcccgt cgatgaccag cgtcacttcg ttatcggtaa aaccttgccc cgcctccaga
     9241 ctttcttgca tcagctcgat atttaatgag aagtagctca acagttccgg taacggtgta
     9301 ccaaacaatt tgcgggagct ttgggcgagc agttgttgcg cggcggggtt ggcgtaatgg
     9361 atcgccagat tgtcatcaat taacaaaata ctgttaatca gcgagttgag gatctgccca
     9421 gcatcgggct gcgtgcctgt tgccataaag cagtctcctg aacaggttgc accattttag
     9481 tgcattatag ctttttacgg ataaaaagcg cgaagcatca gagaattgac ggagaaaaaa
     9541 gcccatgcag agatgggctg aaagtttcca cggcaactaa atcccggcgt tgttgcgccg
     9601 ggtagtacaa cttcaactat tagacgctgt agtacagctc aaactctacc ggatgcggag
     9661 tcatacgcac gcggtcatct tcttcgcgac gtagagcgat gtacgcatca attgcttcgt
     9721 cagtgaacac gccaccggct ttcaggaact cgcggtccag atccagttcg ttcagtgctt
     9781 cttccagaga gcctgcaacc tgtgggatct ctttcgcttc ttctggcggc aggtcataca
     9841 ggtttttgtc catggcttcg cccggatgga tcttgttctt gataccatca agaccggcca
     9901 tcagcagggc agcaaagcac aggtacgggt tagctgccgg atccgggaaa cgtacttcga
     9961 tacgacgtgc tttcggagaa gaaaccaccg gaatacggat agacgcagaa cggttacgcg
    10021 cagagtaagc cagcattacc ggtgcttcat agcccgggac cagacgctta taagagttgg
    10081 tggtcgggtt tgccagggcg ttaatcgctt tagcgtgttt gattacgccg ccaatgtagt
    10141 acagcgcctg ctcagacaga cctgcgtatt tgtcgcctgc gaacaggtta acgccgtttt
    10201 tagacagaga catgtggcag tgcataccgg agccgttatc accgaacatc ggttttggca
    10261 taaaggtcgc ggttttaccg aagcggtgcg ctacgttgtg cacaacatat ttgtagatct
    10321 gaatttcgtc agcttttttg gtcatagtat tgaagcgggt agccacttcg ttctgaccag
    10381 cagtcgctac ttcgtggtga tgggcttcaa ccaccagacc catctgttcc atcaccagac
    10441 acatttcaga acgaatatcc tgagccgagt ctaccggtgg aaccgggaag taaccgcctt
    10501 tcactgccgg acggtgacct ttgttaccac cttcgtattg ggtggaggag ttccatgcgc
    10561 cttcgatatc gtcgatagca acgtgggaac cggagataga tgatccgaaa cggatgtcat
    10621 cgaacaggaa gaattcaggt tctggcccga acagtacggt gtcggcaatg ccagtggaac
    10681 gcaggtaatc ttcggcgcgc ttcgcaatgg agcgcgggtc acggtcatag ccttgcaggg
    10741 tgccaggttc aaggatgtcg caacggataa tcagggtgga gtcggcgaag aacgggtcaa
    10801 tcactgcggt ggatgcgtct ggcatcagca ccatgtcgga ctcgttaatg cctttccagc
    10861 cgccaatcga ggagccgtca aacattttgc cttcttcgaa gaattcagca ttcacctgat
    10921 gagcagggat agtgacgtgc tgttctttac ctttagtatc ggtgaagcgc aaatcaacaa
    10981 acttcacttc gtgctcgttc agcatcgtca gtacgtgttc agcggacata ctttaactct
    11041 cctggattgg tcatggtcgt cgtggtaacg aaatctgcaa ttattttggc cgtgtcgccg
    11101 taaaaaagat aaagcgaaat ctgtgccaac ttttaaattg cccctaaaag gcgttatcat
    11161 gcgcaccatc gtgcaaaagg gctgcaccac gatgtgaatg ttgcaccaat atagtgcttc
    11221 aatggaaaca ttaagcacca tgttggtgca atgacctttg gataaccctt tttatgctcc
    11281 gtgaaagcga tcacaaaggg actctgcaat acttgtttgc ggaggatgtt tgtgatcctg
    11341 ttttgtagtg cgattaatcc gtgtacaata acgcgctatt tctaatgcct gaggcaaagt
    11401 tgtgatcgaa aaattgcgta atatcgccat catcgcgcac gtagaccatg gtaaaaccac
    11461 cctggtagac aagctgctcc aacaatccgg tacgttcgac tctcgtgccg aaacccaaga
    11521 gcgcgtgatg gactccaacg atttggagaa agagcgtggg attaccatcc tcgcgaaaaa
    11581 caccgctatc aaatggaatg attaccgtat caacatcgtt gataccccgg ggcacgccga
    11641 cttcggtggt gaagttgaac gtgtaatgtc catggtagac tcagtgctgc tggtggttga
    11701 cgcatttgac ggcccgatgc cgcaaacgcg cttcgtaacc aaaaaagcgt ttgcttacgg
    11761 cctgaagccg attgttgtta tcaacaaagt tgaccgccct ggcgcgcgtc ctgattgggt
    11821 tgtggatcag gtattcgatc tgttcgttaa cctcgacgcg accgacgagc agctggactt
    11881 cccgatcgtt tacgcttctg cgctgaacgg tatcgcgggt ctggaccacg aagatatggc
    11941 ggaagacatg accccgctgt accaggcgat tgttgaccac gttcctgcgc cggacgttga
    12001 ccttgacggt ccgttccaga tgcagatttc tcagctcgat tacaacagct atgttggcgt
    12061 tatcggcatt ggccgcatca agcgcggtaa agtgaagccg aaccagcagg tcactatcat
    12121 cgatagcgaa ggcaaaaccc gcaacgcgaa agtcggtaaa gtgctgggcc acctcggtct
    12181 ggaacgtatc gaaaccgatc tggcggaagc tggcgatatc gtggcgatca cgggccttgg
    12241 cgaactgaac atttctgaca ccgtttgcga cacgcaaaac gttgaagcgc tgccggcact
    12301 ctccgttgat gagccgaccg tttctatgtt cttctgcgtt aacacctcgc cgttctgcgg
    12361 taaagaaggt aagttcgtaa cgtctcgtca gatcctggat cgtctgaaca aagaactggt
    12421 acacaacgtt gcgctgcgcg tagaagaaac cgaagacgcc gatgcgttcc gcgtttctgg
    12481 tcgtggcgaa ctgcacctgt ctgttctgat cgaaaacatg cgtcgtgaag gtttcgaact
    12541 ggcggtatcc cgtccaaaag taatcttccg tgaaatcgac ggtcgtaaac aagagccgta
    12601 tgaaaacgtg actctggacg ttgaagaaca gcatcagggt tctgtaatgc aggcgctggg
    12661 cgaacgtaaa ggcgacctga aaaacatgaa tccagacggt aaaggccgcg tacgtctcga
    12721 ctacgtgatc ccaagccgtg gtctgattgg cttccgttct gagttcatga ccatgacttc
    12781 cggtactggt ctgctgtact ccaccttcag ccactacgac gacgtacgtc cgggtgaagt
    12841 gggtcagcgt cagaacggcg tactgatctc taacggtcag ggtaaagcgg tcgcgttcgc
    12901 actgttcggt ctgcaggacc gcggtaagct gttcctcggt cacggtgcag aagtttacga
    12961 aggtcagatt atcggtattc acagccgctc taacgacctg actgtaaact gcctgaccgg
    13021 taagaaactg accaacatgc gtgcttccgg tactgacgaa gctgttgttc tggttccgcc
    13081 tatccgcatg actctggaac aagctctgga gttcatcgat gatgacgaac tggtagaagt
    13141 gactccgacc tctatccgta ttcgtaaacg tcacctgacg gaaaacgatc gtcgtcgcgc
    13201 caaccgcgca ccgaaagacg attaatttcg ttttttagtt ataaaaaacc tgccatcgct
//
LOCUS       DAZOYL010000096        13089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZOYL010000096 DAZOYL010000000
VERSION     DAZOYL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.096"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            230..1663
                     /gene="cscA"
                     /locus_tag="ACHUOY_002023"
     CDS             230..1663
                     /gene="cscA"
                     /locus_tag="ACHUOY_002023"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR0682805.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1671..2666)
                     /locus_tag="ACHUOY_002024"
     CDS             complement(1671..2666)
                     /locus_tag="ACHUOY_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0682806.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2897..3115
                     /locus_tag="ACHUOY_002025"
                     /pseudo
     CDS             2897..3115
                     /locus_tag="ACHUOY_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3133..4461
                     /gene="dsdA"
                     /locus_tag="ACHUOY_002026"
     CDS             3133..4461
                     /gene="dsdA"
                     /locus_tag="ACHUOY_002026"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR0682807.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="ACHUOY_002027"
     CDS             complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="ACHUOY_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR0682808.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="ACHUOY_002028"
     CDS             complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="ACHUOY_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR0682809.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7686..8300
                     /gene="evgA"
                     /locus_tag="ACHUOY_002029"
     CDS             7686..8300
                     /gene="evgA"
                     /locus_tag="ACHUOY_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR0682810.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8305..11898
                     /gene="evgS"
                     /locus_tag="ACHUOY_002030"
     CDS             8305..11898
                     /gene="evgS"
                     /locus_tag="ACHUOY_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR0682811.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11954..>13089)
                     /gene="yfdE"
                     /locus_tag="ACHUOY_002031"
     CDS             complement(11954..>13089)
                     /gene="yfdE"
                     /locus_tag="ACHUOY_002031"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR0682812.1"
                     /translation="ESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPPGHGD
                     DTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFRPGTM
                     EKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYPDAPP
                     VRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMAYIAT
                     GKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPRFSSN
                     ILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQARNML
                     IEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
BASE COUNT         3783 a         2981 c         2824 g         3501 t
ORIGIN      
        1 actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct
       61 ttctcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag
      121 ttaacgttaa catttgtgat attcatcgca tttatgaaag taagggacta tatttttata
      181 aaagttaacg ttaacaattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc
      241 tcgattgcat gcggcgcaaa acgcactagc aaaacttcat gagcaccggg gtaacacttt
      301 ctatccccat tttcacctcg cgccacctgc cggatggatg aacgatccaa acggcctgat
      361 ctggtttaac gatcgttatc acgcgtttta tcaacatcac ccgatgagcg aacactgggg
      421 accaatgcac tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat
      481 tgcgctagcg ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga
      541 tgacaatggt gtcctctcac ttatctacac cggacacgtc tggctcgatt gtgcaggtaa
      601 tgacgatgca attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga
      661 gaaacagggt gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt
      721 gtggcgtgaa gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg
      781 gcagatcctg ctttatcgcg gcagttcatt gcgtgaatgg accttcgatc gcgtactggc
      841 ccacgctgat gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga
      901 tcaacattat ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa
      961 tcgctttcaa agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc
     1021 cgggcatttt actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc
     1081 gaaggatggt cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc
     1141 aaaacgtgaa ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg
     1201 caaacttcta caacgcccgg ttcacgaagc tgagtcgtta cgccagcagc atcaatctgt
     1261 ctctccccgc acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat
     1321 tcagttgcag tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac
     1381 tggaatgcgg ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc
     1441 acacgagaat ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct
     1501 aaggatattt atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat
     1561 gagtagtcga atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg
     1621 agtggctgtg ctgcaacatg gagcactctg gctactgggt taacataata tcaggtggaa
     1681 caacggatca acagcggaca agggatccgc gtcactcttc ccccttcacg accttcaata
     1741 atatgcaatg cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc
     1801 ggtaaaaaca gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatcctgc
     1861 gggatacgta cccccttcgc caggagaacc tgataagcga caaaggctgc acgatcgtta
     1921 ccacatatca gaacatcaaa atctggtttg cccgatttga agtgggcatt gagtaaactt
     1981 gcgagatcgg tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga
     2041 tctcgtccag catcacgcca ggcctgctca aatccctgcc gacgataccc tgttgccaac
     2101 gcactttccg gtagccagaa gcataacggt tgacgatagc ccgccgcgag caaatgctgt
     2161 gttgattcat attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc
     2221 gccacacagt tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgt
     2281 cgcagcccca ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc
     2341 gcgcgggcag cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg
     2401 ctggcggttt gctcaatggc aagcagaata tcaacagaga aaggagtggt agccgtgtcc
     2461 tgcgccagca cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa
     2521 agatcaggaa cataattcag ggtctggatt gcctgcaata cgcggtcacg cgttgcagga
     2581 cgcacagatt ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccagg
     2641 cgtgcgacat cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag
     2701 cagaaacaga cgtcactggc gagatccaac gccctatcac ctgacacagc aatacaataa
     2761 aaaataacaa taattcccgg acaattgtcc ccaattccgc ctctgttctc gccaacgagt
     2821 aaccatatag gcaagtaagt aaaacatgcc aactggcatc gccaacctgg agaagaaaat
     2881 ataaatatgg atcattgtgc tgccgctgta tcccgacatc agcccggaaa ttattgcgat
     2941 tgctatcggt tcaggtgcaa ttggctgcac gatcgttacg gactcgcttt tctggctagt
     3001 gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt
     3061 tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca
     3121 aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta
     3181 aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct
     3241 gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta
     3301 tcccgttttg caccctatct ggcaaaagca tttgctgaaa ctgctgccac tggggggatt
     3361 attgaatcag aactggttgc cattccagct atgcaaaaac ggctggaaaa agaatatcag
     3421 caaccgatca gcgggcaact gttactgaaa aaagatagcc atttgcccat ttccggctcc
     3481 ataaaagcac gcggcgggat ttatgaagtc ctggcacacg cagaaaaact ggctctggaa
     3541 gcggggttgc tgacgcttga tgatgactac agcaaactgc tttctccgga gtttaaacag
     3601 ttctttagcc aatacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc
     3661 attatgagcg cccgcattgg ctttaaggtg acagttcata tgtctgctga tgcccgggca
     3721 tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat
     3781 ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt
     3841 gatgacgaaa attcccgcac gttgttcctt gggtattccg tcgctggcca gcgtcttaaa
     3901 gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata accctctgtt tgtctatctg
     3961 ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg ggcttaaact ggcgtttggc
     4021 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
     4081 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
     4141 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
     4201 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
     4261 caggaagaag gtattcgtct tgaaccttcg gcactggcgg gtatggccgg acctcagcgc
     4321 gtatgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca actgcgtaat
     4381 accactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
     4441 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
     4501 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
     4561 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
     4621 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
     4681 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accataatat
     4741 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
     4801 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
     4861 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
     4921 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
     4981 ccctgaaaaa actgcggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     5041 gaacgccagt aatagcaaac cgcatacatc aaaaaactaa atgtcactaa caaccgcatg
     5101 tctattttgt tgccataacg tcctatcaaa ggtgaaatta atagtggcat gatgccgatg
     5161 ggcgcataag caagtccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     5221 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc atgtgatact cacaatacca
     5281 atggtgaagt tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     5341 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     5401 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     5461 ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct
     5521 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     5581 aaaaatatcc aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat
     5641 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggc
     5701 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaacccc
     5761 tgaacgactc taaaaaatat cagcacatca agattggtcg ataggctaca cattaatgaa
     5821 gacagcgaaa aaaaggttac tgaaagtaaa aataatctta attcgcctat tctttgtgcc
     5881 aacctgccag taacagggat cgcaatggca tttgctacac caaacgaggt gataacccag
     5941 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     6001 atagtggaat ccaacatttg cataaatgtc gctaatgaca atgcaatagt gacgcaccat
     6061 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     6121 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     6181 tactggtata agccggcatg gaggtcacgg ttgaagccag ctcaggcatc tcagcaatgt
     6241 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     6301 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     6361 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     6421 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     6481 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     6541 gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa
     6601 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcgcca
     6661 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     6721 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     6781 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     6841 tcgctaaagg cacacgacgg ttataatctt ctaaggattg ttgatactga atacgtgctg
     6901 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     6961 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     7021 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     7081 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     7141 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     7201 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     7261 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     7321 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     7381 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     7441 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
     7501 catcggtata atccctgatt ttattgttga catttcattt atgccgacta tttatatggt
     7561 atacttgtcg aattatctta aaggaagctc agattttctt atttttattg agaaaatgag
     7621 atgacgcctt atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat
     7681 aatctatgaa cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt
     7741 tattgatcaa aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc
     7801 agcgggtgga aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta
     7861 acggtatcct ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg
     7921 tctccgctaa aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt
     7981 tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg
     8041 gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc
     8101 aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca
     8161 aggataataa tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacttata
     8221 aaagtcgcct gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcac
     8281 aacgtaacaa aatcggctaa ccacatgaag tttttaccct atatttttct tctctgttgt
     8341 ggtctttggt cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt
     8401 agtaacaacc gtgtcatact tgatccagta cgtctgagca acaaggaatt acgttggtta
     8461 gcgagcaaaa aaaatcttgt gattgcagta cataagtccc aaacggctac gttgttgcat
     8521 accgattcgc agcaacgagt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga
     8581 acgttaaata tcaaattaac actccgggaa tacgccgatc atcaaaaagc aatggacgcg
     8641 ctggaagaag gtgaagtcga tatagtgtta tcacatttag tagcttcgcc gcctcttaat
     8701 gatgacattg ctgcaaccaa accactgata attacctttc cggcgctggt aaccaccctt
     8761 cacgattcaa tgcgaccgct tacctcatca aaaccagtaa atattgctcg agtagcaaat
     8821 tatccccctg acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca
     8881 aatttatatc aggcattagc atccgtctca gccggacaga atgattactt tattggtagt
     8941 aacatcatta ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa
     9001 tattataact caccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcgttctt
     9061 aatgaagtac tcaatcgatt tgttgatgct ttgacaaatg aagttcgcta tgaagtatca
     9121 caaaattggc ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa
     9181 catgaaaaac aatggattaa gcagcatccc gatttaaagg tgctggaaaa tccttactcc
     9241 cctccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggatattctt
     9301 aatattatta ctttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
     9361 catgctggaa cacagctgaa tcccggcgga tgggatatat tacccggtgc tatttatagt
     9421 gaagatagag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
     9481 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
     9541 ccatattatt atgagcttca ttcgcaatta aaagagatgt atccggaggt agagtggata
     9601 aaagtcgata acgccagcgc tgcatttcac aaggtcaagg aaggcgaact tgatgctctg
     9661 gtcgcgacac agttaaattc acgttacatg atcgaccatt actatcctaa tgaactttat
     9721 cattttctta ttcccggcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
     9781 ccggaactta aggatattat taataaagca ctgaatgcaa tccccccaag cgaagttctg
     9841 cgcctgaccg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctctat
     9901 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
     9961 tggggatttt acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
    10021 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
    10081 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
    10141 tactacaaaa atgcaatgtt accattagaa aacagtgact ccccctttaa agatgttttt
    10201 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggtt
    10261 tttgaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
    10321 ctgccagcca gcgatcatgc tgtttatatt tgtggttggc aagatattac agagacgcgt
    10381 gatttaattc atgcactcga agtagagaga aataaagcga tcaatgcaac tgtcgcaaaa
    10441 agtcagtttc tggcaacaat gagtcacgaa ataagaacac caataagctc cattatgggc
    10501 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
    10561 cttgcctact ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
    10621 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
    10681 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaatttgc
    10741 agcagtacat ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc
    10801 ttatcaaatt tactgagcaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
    10861 acctccctgg ttcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
    10921 ggaagtggat tatcgcagga agaacaacaa caactcttta aacgctacag ccaaacaagt
    10981 gcaggtcgcc agcaaacagg atctggctta ggcttaatga tctgcaaaga attaattaaa
    11041 aatatgcagg gcgatttgtc attagaaagt catccaggca taggaacaac atttacgatc
    11101 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacacccc
    11161 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
    11221 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
    11281 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
    11341 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
    11401 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
    11461 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
    11521 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
    11581 aataatacgg cgaacgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
    11641 acgcataaag atctacccgc tgcgtttcaa gcactagaag ctggcgataa cagaactttc
    11701 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
    11761 aatattagcc atcagttaga aataacacct gtttcagatg acagtaagcc tgaaattctt
    11821 cagttgctga actctgtaaa agaacacatt gcagagctgg accaggagat tgctgttttc
    11881 tgtcagaaaa atgactaaat agcggctccc acaatgttct aatgtgggag ctatttacca
    11941 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
    12001 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
    12061 gcatcattat tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa
    12121 tggcctcagc cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta
    12181 accaaacttc agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct
    12241 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
    12301 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
    12361 tatcctgagt atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt
    12421 gtggtgactt ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
    12481 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
    12541 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa
    12601 cacgcactgg cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa
    12661 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
    12721 aagcatatat gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt
    12781 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
    12841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
    12901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
    12961 catgacctgg tggctcaact ttaattaccc ttgcgcccat attacaaaga agttgagttc
    13021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
    13081 tgctttcat
//
LOCUS       DAZOYL010000097        13051 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZOYL010000097 DAZOYL010000000
VERSION     DAZOYL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13051
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.097"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            491..4951
                     /gene="gltB"
                     /locus_tag="ACHUOY_002032"
     CDS             491..4951
                     /gene="gltB"
                     /locus_tag="ACHUOY_002032"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR0682813.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4964..6382
                     /gene="gltD"
                     /locus_tag="ACHUOY_002033"
     CDS             4964..6382
                     /gene="gltD"
                     /locus_tag="ACHUOY_002033"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR0682814.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6566..6970
                     /locus_tag="ACHUOY_002034"
                     /pseudo
     CDS             6566..6970
                     /locus_tag="ACHUOY_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            7062..7367
                     /locus_tag="ACHUOY_002035"
     CDS             7062..7367
                     /locus_tag="ACHUOY_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0682815.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(7443..7907)
                     /gene="nanQ"
                     /locus_tag="ACHUOY_002036"
     CDS             complement(7443..7907)
                     /gene="nanQ"
                     /locus_tag="ACHUOY_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR0682816.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(7904..8779)
                     /gene="nanK"
                     /locus_tag="ACHUOY_002037"
     CDS             complement(7904..8779)
                     /gene="nanK"
                     /locus_tag="ACHUOY_002037"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR0682817.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8776..9465)
                     /locus_tag="ACHUOY_002038"
     CDS             complement(8776..9465)
                     /locus_tag="ACHUOY_002038"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR0682818.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9513..11003)
                     /gene="nanT"
                     /locus_tag="ACHUOY_002039"
     CDS             complement(9513..11003)
                     /gene="nanT"
                     /locus_tag="ACHUOY_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR0682819.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(11113..12006)
                     /gene="nanA"
                     /locus_tag="ACHUOY_002040"
     CDS             complement(11113..12006)
                     /gene="nanA"
                     /locus_tag="ACHUOY_002040"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR0682820.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12128..12919)
                     /gene="nanR"
                     /locus_tag="ACHUOY_002041"
     CDS             complement(12128..12919)
                     /gene="nanR"
                     /locus_tag="ACHUOY_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR0682821.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         3146 a         3555 c         3484 g         2866 t
ORIGIN      
        1 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaat gcagtataaa
       61 atgctggttt tctcgtcaat tcaaggcagg ataagggtta acacgccttt atgacagtca
      121 ggaattgact gtttctctaa cgacttccct tttagcctta aagataaaat ctattttaat
      181 ttcagccatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
      241 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
      301 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
      361 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaacc cccgtcgcca
      421 cgctctttct gtacccgtgc gcagcggttc ggtagtgggg ttcccgcaga gcctggggga
      481 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
      541 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
      601 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggtgacg gttgcggctt
      661 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
      721 agcgaaaaac tacgccgtcg ggatgctatt cctgaataaa gatcctgaac tcgccgctgc
      781 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
      841 tgatgtcccc actaacgaag gcgtactggg tgaaatcgcc ctctcctctc tgccccgcat
      901 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtccg cgcgatatgg agcgccgtct
      961 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
     1021 tagcctatcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
     1081 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
     1141 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
     1201 taacggtgaa atcaacacca tcactggtaa ccgccaatgg gcgcgtgcgc gtacctataa
     1261 attccagacg ccgcttatcc ctgatctgca cgacgccgca ccgttcgtca acgaaaccgg
     1321 ctctgactcc agttcgatgg ataacatgct ggaactcctg ctggcaggcg ggatggatat
     1381 catccgcgcc atgcgtctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
     1441 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggcccggc
     1501 gggcatcgtg atgtccgacg gtcgttttgc cgcctgtaac ctcgaccgta acggtctgcg
     1561 tccggcgcgc tacgtcatca ccaaagataa gctcatcacc tgcgcctctg aagtcggtat
     1621 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
     1681 ggttatcgac acccgcagtg ggcatattct gcactcggca gaaaccgatg acgatctgaa
     1741 aagccgccat ccatataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
     1801 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
     1861 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
     1921 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
     1981 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
     2041 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
     2101 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
     2161 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
     2221 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctgtg
     2281 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgctg ctggtgctct ccgaccggaa
     2341 tatcgctaaa gatcgcctgc cggttccagc cccgatggcg gttggcgcga tccagacccg
     2401 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
     2461 cgatccgcac cacttcgccg tgttgctggg cttcggcgcg acggctattt atccatacct
     2521 tgcctatgaa acgctgggcc gcctggtaga cacccatgcg attgccaaag attatcgtac
     2581 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
     2641 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
     2701 cgatgatgta gtgggcctgt gcttccaggg ggcggtcagc cgcattggtg gggcaagctt
     2761 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgtaagcc
     2821 catcagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
     2881 ggacgtggtg cgcacgctgc aacaagcggt acaaagcggc gagtacagcg actatcagga
     2941 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
     3001 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
     3061 cttcgatacc gctgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
     3121 ggaagcgatg aacagcatcg gcggtaactc aaactccggt gaaggcggtg aagatccggc
     3181 gcgctacggc accaacaaag tgtcgcgcat caagcaggtg gcttccggtc gctttggggt
     3241 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
     3301 gccgggcgaa ggcggtcagt tgccgggtga taaagtcact ccttacatcg ccaaactgcg
     3361 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
     3421 cgaggactta gcgcagctca ttttcgacct caagcaggtt aacccgaaag cgatgatctc
     3481 cgtgaagctg gtttccgaac cgggcgtagg caccatcgcg actggcgtgg caaaagctta
     3541 tgccgattta atcaccatcg caggctatga cggtggcacc ggcgcaagcc cgctgtcgtc
     3601 agtgaaatac gcaggttgcc cgtgggagct ggggcttgtt gaaacccagc aggcgctggt
     3661 tgccaatggt ctgcgtcata agatccgttt gcaggtcgat ggcggcctga aaaccggtgt
     3721 tgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
     3781 ggtcgcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg caacgggtgt
     3841 ggcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
     3901 gaattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggggtaac
     3961 gcggctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
     4021 caaacagcag aaactggcgc tgtcgaagct gctggagacc gccgaaccgc atccaggtaa
     4081 ggcactctac tgcaccgaaa acaacccgcc gtttgataac ggcctgctga acgcgcagtt
     4141 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
     4201 ccgcaatacc gatcgctccg tgggcgcgtc gctttcaggc tatatcgccc agacgcacgg
     4261 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
     4321 tttcggtgta tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
     4381 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
     4441 ccgcagccat gaagcaagta ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
     4501 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
     4561 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
     4621 tctgggtaaa accggcgtta acttcggtgc gggcatgacc ggcggcttcg cttacgttct
     4681 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgagg tcttaagcgt
     4741 tgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
     4801 tactggctct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccactaaatt
     4861 tgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcatc gtagtcgtag
     4921 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
     4981 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgcaaaat
     5041 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
     5101 ctgcctgtcg tgcggcaacc catactgcga gtggaaatgt ccggtacaca actacatccc
     5161 gaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcggaac tgtcgcacca
     5221 gaccaacacc ctgccggaag tttgcggacg agtctgcccg caagaccgtc tgtgcgaagg
     5281 ttcctgcact ctgaacgatg agtttggcgc ggtgaccatc ggcaacattg agcgttatat
     5341 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
     5401 taaaaaagtg gcgattatcg gcgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
     5461 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
     5521 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
     5581 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tgcagctgga
     5641 cgatctgctg agtgattacg atgccgtctt ccttggcgtc gggacttatc agtcaatgcg
     5701 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
     5761 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
     5821 aggcaaacgc gtggtggtcc ttggcggtgg cgacactgcg atggactgcg tgcgtacgtc
     5881 cgtgcgccag ggagcgaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
     5941 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
     6001 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
     6061 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
     6121 cgaacatatc gttccggcag atgcagtgat catggcgttt ggtttccgtc cacacaacat
     6181 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgccccgga
     6241 aggcagcgac aacgccttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
     6301 ccgtggttcc gatctggtgg taaccgctat tgccgaaggt cgtaaggcgg cagacggtat
     6361 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
     6421 gctgaaattc tcctttcttt ctggaaagcg cctcgcataa tctgaagaaa accaatgtaa
     6481 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
     6541 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
     6601 ccagcaaatc cacgacggta ttattcacct ggtcgatagc gcccggacgg aaacggtacg
     6661 tagcgttaac gcgctgatga ccgcgacata ctgggaaatt ggccgacgaa ttgtcgaatt
     6721 tgaacaaggt ggcgaggcaa gggctgcgta tggtgcgcag ctaatcaagc gactatcaaa
     6781 ggatttaagt ctaaggtata agcgtggttt ctctgcaaaa aacttacgcc aaatgaggct
     6841 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
     6901 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
     6961 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
     7021 tcggtttgta tattcatgca aataaacgaa ggaaagttgc cgtgatttac attgaacagc
     7081 ttgaattaat ccataaaagt ggtgatgtgt tgtatccagt aaaaataacc agaaaatcat
     7141 caggaaaaac tgccttccac cttgttccat tcggcttaaa taagactcac gaccttctag
     7201 aggtggaaga tgcttcagaa gcaattcggc tggtgattga tgaacgtcat tccattcgct
     7261 gcagtacact tacagcaact attacgaata aaaagggcaa gcgaattaag cggacaggta
     7321 tatacagtat caagggtgtt aacataaaag aatacaacgt caggtaatgg cacagagcgg
     7381 tccgtcagtt caggtttggc tctgtgccaa agatgggtca ggtcacacct gatctaataa
     7441 atttaagcca ttaaatcagc cttaaccttc accaccacct ttttaatctc acccggctcg
     7501 ccaacaacgc atcccggctt atgcggctct cctagcataa acacggcgaa cattcccggt
     7561 ttcaggatga tggcttgctc gttttcaatg gtgctgcaaa gctggtaatc atcctcatgg
     7621 tggaactctt cacactgacg cgcagtgcct gccatgccaa acagaatccg ttcttcaccg
     7681 tttaataaca gctggatatc aatgtattgc tcgtgcagct ccgctttttt ctcaacgggc
     7741 gattgagtgt taaacgtcat gacattcata aaaatattgt cgccctgtaa ttcgtaacga
     7801 cccggcgctt tttcttgcgg tctggcagct aatgccagcg ttaacgcgtc ctgtaacgca
     7861 ggatgtaacc cagcagacgg taatgaccgt acttcaccca tcatcataat ttttctccct
     7921 gggccaacag cgctgcccca agtaaacctg catcatggcg gtagtgcgcc gccagtaaat
     7981 caacatgaaa taccgctggc tcctgcgcca gatacgtttc caccaacgcc agataccctt
     8041 ctgccagacc aacgctgcca ccgaccacca cgcactggca atcagttgtg gctttaatat
     8101 cagcgatcag ccttgccagc acatgtgcgg agcggtgaat cagttgctgc gcctgctcgt
     8161 cgccctgccc ggcgcgtgtg aaaatagttc tcgcatccgc gcccatgagt tcaccttgcg
     8221 ctgccgctgc aataccgcga ccagaagcaa tcgcttccac gcaacctgtg cgtccacagc
     8281 cgcagactgg gccgtgtgga tcggcaagcg tatgcccgat atgccccgcc agaccgccag
     8341 ggccggtaag cagtttaccg ccgctcacta caccgccgcc gacgccagtg gaaacggtga
     8401 taaacaccat ctcggttaca tcgccttcca gcgcctgata ctctgcccat gctgcggcct
     8461 gcgcgtcgtt aatggcaatg gtcggcaaat tggtaagttg ttccagcgtt ttgactaacg
     8521 gaaagtgtag caatccaccg agattatgcg gattcagcgc cagcaaactg ccgtcgcgaa
     8581 taattccggt tgaagcgatg gcaacccgct gcgcatgagc ttgcaacgga gagactaatg
     8641 cggataaggc atcacgcaag gcttctggtg tctggctggc tggcgtagga agttcacgac
     8701 gatcgcggat ctgcccgtca gcgccaatca gcgcggcggc aagtttagta ccgccgatat
     8761 caatcgccag tgtggtcata gcaccgcctt tttcatcgct gtgttgtacc actgacaaat
     8821 gtgttcaaga cgcgtaatgg cagaaccgac cgtcaccgcc cacgcgccgt ggcgcatcgc
     8881 atctgccgcc tgagcaggcg tgttgtaacg cccttcggca atcacccgac atccggcttc
     8941 gctcaacgtt ttcaccagcg ccagatccgg ctcttctggc gtttcaggcg tggtatagcc
     9001 agaaagcgta gtgccaataa tttcggctcc cagcttttgg catgccaggc cgtcttccgg
     9061 cgttgagcag tcggtcatcg ccagtaaacc gtgatgatgt atacgtgcca gcagcgtttc
     9121 aacaggcacc ggacgcgggc ggtcggtgcc gtcaatggcg ataatgtccg cgcccgcctg
     9181 cgccagcgca tcgacatctt caatataggc cgtgatgcgt accggagaat cctccagatc
     9241 gcgtttcaca attccaataa tcggcacgct caccaccgca cgcgtggctt gcagatttgc
     9301 cacaccttcg atgcgaatgg caaccgcgcc cgcctgctct gccgctaatg ccatggctgc
     9361 gacgatttcg ggtttatcga gcgggctgtc aggaatcggc tggcaggaga caatcaggcc
     9421 accgttagca gcgatttttt gatccagttg tgcaagtaac gacatacatc ttcccttagc
     9481 gaaaggcccg gtacatagac cgggcaacag gattaacttt tggttttgac taaatcgttt
     9541 ttggcgctgc caaatggcac ggcaccgcta aatggtttgc cgtcgatagc gtcatgagta
     9601 cgcaatgctt ccgggcgcag ccaacgctga acgcgggaag gcatatccag accaatcagc
     9661 aggatcacca caaatgtcag gctgaacgag agcgatgcca gcgcagtacc cagatccaga
     9721 cgttgagcga tcaacgcgcc gatgattggg gccagtgcgc cacccaatgc gccaacgttg
     9781 taggtaaagc ccaggcccgc tgcacgctgg tcggtatcga aataaccgcc aatcagtttc
     9841 ggtaagatcc cggcgatccc ttgtccaagc atttgctgga agaacagtaa cagaccgagc
     9901 acccagacgt ttgcgccgcc aatcgcaaat accggaataa tcagcagctg cgaggccagc
     9961 aggctacaaa cgtacgcttt gcgggttccc agccagtcac cgaggaagcc tcctacgcag
    10021 catcccacgg ccgcgccaaa gccactaaag aacagcacat tggcgacagt atgcgggtta
    10081 taagccagat cggttttcag atacgttggc agcagcgcct gaatcggcca tgagtagagg
    10141 aaagcaaaca acacgaccac catcagcatt acgcccgttg gccagcgttt gcctgtactc
    10201 tgcaccataa agctgataaa gattgcggcg cataacagcc caagaacagc gacgatcgcg
    10261 gcattttgca ggttaccggc gaagcagaac cacagcgcag tagccgccgc cagtgtcatt
    10321 acgatattgg caatgcgatg ttcaccacgg tagagaatat ccaccattgt gcgtactggt
    10381 gctttacctg cgtgtttctc tttccagtct tccgcttccg ggatgttttt acgcagccag
    10441 agagcaaaga tgattggcaa aatgccgata aagaacagcg cacgccaacc ccagaccgga
    10501 accaccaggc tatagacctg agcggcaacg acggccccca cagagaagcc tgaaatcaaa
    10561 aaaccactgg ctttgttacg cagatgtttt ggccagcttt caatgacata ggtggcgctg
    10621 gaaccgtatt cacccgccat ccccatgccg atgaccagac gagcgataaa catggtgatg
    10681 tagcctggcg caaagccgca ggccagcgtc ccggccgaga agagaacgat gctggtgacc
    10741 attgccagac gacgcccgta gcggtcaccc atagcgccga gcatcaggcc gccgaaccag
    10801 cgagagataa aggctgcgga gatcagactt gccgcctgca ccgtcgtcag cccgaattca
    10861 ccctgtactt cggtgagtac cagggcgatt aaaacgaaat caaaaccgtc aagcagatat
    10921 cccaaccagg cagcggaaaa tgcgcgccat tgtgcacggt tgagatggcg ataccacggg
    10981 atattctggg ttgtagtact cattgtgagt ctcccgcggt gggcgatgcc cacacgcttt
    11041 ggtatgaaaa ttgtagggta cagatgcgtt tatttcccct cacccggtag gggcgagcga
    11101 ggggaaaaca actcacccgc gctcttgcat caactgctgg gccagcgcct tcagttctgg
    11161 cagatatttt tcatctaccg gtccaaacgg tttgcggcac agcggcacag aaacgacatc
    11221 catataatgc aggacagttt tcaggccgcg gaatacgcca gttttgatca gtaaatcaat
    11281 gactttgttg cactcagttt gcagtttctg cgcggtctgg atatcgcctt ctttcagcgc
    11341 cttcacgatc ccctgatatc gccagcccat gatgttgtaa gtactgccga taccaccatc
    11401 agcgcctgcc agcaagccgg aagcgaagat ttcgtcgtaa ccgttataga gcaccagatc
    11461 aggatgttca cgacggatct gttccatctg atagagatcg ccagaagtct gtttcagcgc
    11521 accgacgcca ggcaacgtaa ccagggtgtt gatctgatcc agggtcagtt ttaccccact
    11581 cagggctgga atgttgtaca caaccatcgg caaaccatcc gctgaatcaa taattgcccg
    11641 atagtgatcg cagtgttctt caaagctgaa aggatagtag aacggcgtga cggcggagac
    11701 ggcatcgaag ccataacgtt tagccgatgc cgcaagttgt tggctttcgg cggtgctgac
    11761 gcaaccgacg tgggcgatga gtttaatctt acctttcgcc tcttcggcga cgatttccag
    11821 tacctgttca cgctcggaaa ggctttgtac aaaggcctcg ccggtcgaac cacccacgta
    11881 taaaccgtcg atgccctgct gaatattgaa ctgaaccagg cgacgcagac tcgctttatc
    11941 cagtgcttgt tgttggtcaa aaggagtcag gagtgcagcc attacgccac gtaaattcgt
    12001 tgccataaat acctctgaag tgatacttgt ctgataaacg atataccttt atacctgtta
    12061 taccagatca attaagcaac accccataca gaaagcttat aatgcgatct gcttcactaa
    12121 agtggcatta tttctttttg ttggtggtct gaccgaaagc gtgccaggta gcagagacgc
    12181 tgttgagatg cgattgcaac gcacgatcgg cttcgtcagg atcatgacgg cggatcgcat
    12241 caacgatcgc aatatgctgt tgatagctaa cgttgttatg ctcgtgcaga gcctgatcag
    12301 ccacggttgg acgtgcggcg atcagccagt cgagcagggc aacgtggatc gccatgaaga
    12361 ttgggttacc agggatctcc gccagcacgc ggtggaaatc aacgtctgaa cgaatgaatg
    12421 ccgcgttgtt atccagcgac tgactgttga tttccagtgc ttttgccagc aaatcgattt
    12481 gctcatcggt ggcatgttca gccgcatagc gcaccagact ggattcaaag aacagacgta
    12541 attgttcgaa atgggcaatc ccaccgggat gagaaaggaa atctttcgcc atgccggaaa
    12601 actcaccgat gatagtgtcc gcagaaggac gcgagacgcg agcgcgttcg ccgttgttta
    12661 tttgcaccag acctttgcgt tttaacgctg ccagcgcttc gcgcacagaa ggacgcccga
    12721 cgttaaagaa cgccatcagt tcgcgttcag acggtaattg ttcaccttcg ccaaattcac
    12781 gacggcggat catctgttcc agctcttctt ccaccatttc ggagagtttt ttacgcgcca
    12841 gcgggcggct acgcaagttg cgaccaattg caggtgaaga atcttcggtt tgcgaatcaa
    12901 atgcgttcat aaggcccatt ctgtaaggtc agtgtgatta acatcatcag tgacatccta
    12961 tcacaggatt gaaagtaggg gaaaatggca ggtttttctc tttgtgcctc atcattacca
    13021 taattaacgg aataattaac tattgcgaaa a
//
LOCUS       DAZOYL010000098        13002 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZOYL010000098 DAZOYL010000000
VERSION     DAZOYL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.098"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            142..378
                     /gene="lpp"
                     /locus_tag="ACHUOY_002042"
     CDS             142..378
                     /gene="lpp"
                     /locus_tag="ACHUOY_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR0682822.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(441..1445)
                     /gene="ldtE"
                     /locus_tag="ACHUOY_002043"
     CDS             complement(441..1445)
                     /gene="ldtE"
                     /locus_tag="ACHUOY_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR0682823.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(1594..2010)
                     /gene="sufE"
                     /locus_tag="ACHUOY_002044"
     CDS             complement(1594..2010)
                     /gene="sufE"
                     /locus_tag="ACHUOY_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR0682824.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(2023..3243)
                     /gene="sufS"
                     /locus_tag="ACHUOY_002045"
     CDS             complement(2023..3243)
                     /gene="sufS"
                     /locus_tag="ACHUOY_002045"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR0682825.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(3240..4511)
                     /gene="sufD"
                     /locus_tag="ACHUOY_002046"
     CDS             complement(3240..4511)
                     /gene="sufD"
                     /locus_tag="ACHUOY_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR0682826.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(4486..5232)
                     /gene="sufC"
                     /locus_tag="ACHUOY_002047"
     CDS             complement(4486..5232)
                     /gene="sufC"
                     /locus_tag="ACHUOY_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR0682827.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(5242..6729)
                     /gene="sufB"
                     /locus_tag="ACHUOY_002048"
     CDS             complement(5242..6729)
                     /gene="sufB"
                     /locus_tag="ACHUOY_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR0682828.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(6738..7106)
                     /gene="sufA"
                     /locus_tag="ACHUOY_002049"
     CDS             complement(6738..7106)
                     /gene="sufA"
                     /locus_tag="ACHUOY_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR0682829.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(7654..7842)
                     /locus_tag="ACHUOY_002050"
     CDS             complement(7654..7842)
                     /locus_tag="ACHUOY_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR0682830.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(7942..8352)
                     /gene="menI"
                     /locus_tag="ACHUOY_002051"
     CDS             complement(7942..8352)
                     /gene="menI"
                     /locus_tag="ACHUOY_002051"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR0682831.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(8349..11405)
                     /locus_tag="ACHUOY_002052"
     CDS             complement(8349..11405)
                     /locus_tag="ACHUOY_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR0682832.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            11794..12906
                     /gene="ydiK"
                     /locus_tag="ACHUOY_002053"
     CDS             11794..12906
                     /gene="ydiK"
                     /locus_tag="ACHUOY_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR0682833.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT         3041 a         3497 c         3212 g         3252 t
ORIGIN      
        1 aaaacccagc gttcgatgct tctttgagcg aacgatcaaa aataagtgcc ttcccatcaa
       61 aaaaatattc tcaacataaa aaactttgtg taatacttgt aacgctacat ggagattaac
      121 tcaatctaga gggtattaat aatgaaagct actaaactgg tactgggcgc ggtaatcctg
      181 ggttctactc tgctggcagg ttgctccagc aacgctaaaa tcgatcagct gtcttctgac
      241 gttcagactc tgaacgctaa agttgaccag ctgagcaacg acgtgaacgc aatgcgttcc
      301 gacgttcagg ctgctaaaga tgacgcagct cgtgctaacc agcgtctgga caacatggct
      361 actaaatacc gcaagtaata gtacctgtga agtgaaaaat ggcgcacatt gtgcgccatt
      421 tttttgtctg ctatttacca ctactgcgtc gcgcgtaaca tattcccttg ctctggttca
      481 ccattctgcg ctgactctac tgaaggcgca ttgctggctg cgggagttgc tccactgctc
      541 accgaaaccg gataccctgc ccgacgatac aacgctttat cgactaactt ctgatctaca
      601 gccttattgt ctttaaattg cgtaaagcct gctggcagtg tgtatggcat tgtctgaacg
      661 ttttgctgtt cttctgctga tagtggtcga tgtacttcaa cataacgcat cccgttaggt
      721 tccacggaat atttcaccgg ttcgttgatc actttcaccg gcgttcccgt ccgcacgctg
      781 gagaacaagg ctttaatatc cggtgcattc atgcgaatac accctgaact gacgcgcaaa
      841 ccaacgctgt ccggcgcact ggtgccatga atgaggtatt cgccattacc atgcgcgagg
      901 cgcagtgcgt aacgtcctag cgggttattt ggtccggcag gaacgactgg cggtaattta
      961 atgccacgct ccagcgaacg ctgacgaatg cctgccgtag gcgtccaggt tgggttaggg
     1021 attttctgcc caacacgcgt ttccatcacc ggcgtttcca gcccctgcaa tccaatacct
     1081 attggataaa cctgcacaat attttctccc ggcggataat aataaaggcg cagctctgca
     1141 aggttaacga taatcccctg acgcggtgca tcaggtaata acagttgtga aggaatagtt
     1201 atcgtcgtac caggttttgg caccgggacg atagtgttat tggcttcaag gatcaacatt
     1261 gccgcagtat caaaacgtcg ggcaatagcc tgaaggtttt tatccccttc ttgcaccgta
     1321 tacgtttgat tttgcccaac cagtcggctt ccggttggtg gtagcggata atcaaccgcc
     1381 caggcagcct ggatggcgct aaaagcgccg ataagcgtga gtgtaagcaa agacgcgcgt
     1441 ttcattgtaa acctcctgta tttgccggag actcacgctg aaacgtcgga tggcgcttat
     1501 gttcacctga aaccaaaaca ctcctgtgca ggtcagtgta aacattgacc atccggcaat
     1561 gtgagccaac cggatgaaag ctgtcccttt agtttagcta agtgcagcgg ctttggcgcg
     1621 aattgcgcga atcatcgctt ccagaccttg tgaacgagat ggggtgagat gttgggtgag
     1681 tgccattttt tcaaaccacg gacgcacatc gaaactgaca atatcctgcg gcgtcatctg
     1741 atcgtagaga ataaagacta ccgcaataag ccctttcaca atcgccgcat cgctgtcgcc
     1801 ctgcaattca ataattccgt gcgcattctg gcgcatgaca atccacacct gactctggca
     1861 gccctgaatg ctattttgtg ggcttcggtc ttcgtcgcgt aattcagtca gacgctggcc
     1921 cagctcaata atgtagagat atttctcttc ccagttggcg cagcgtaaaa aattacgcag
     1981 caatttttct ttatccggca ataaagccat agtgcctccc tgttatccca gcaaacggtg
     2041 aatacgttgc aggccggtca ccagacgatc cacttcttca tgggtgttat acatggccag
     2101 cgacgcccga cacatcgcag ggacgttgta ataggccatt aatggcatag cgcagtgatg
     2161 tccggtacgc acagcaatgc cgtaattatc gagaaaactg ccaacatcat aggcgtggtg
     2221 tttaccgaga ttaaaagcaa taacgccaag cctgttttgt gggccataga gagtgagatc
     2281 cggtacagat tccagctgtg atagcgcata atgcatcaga ttctgttcat actcggctat
     2341 gttattaagc cccagcgccg aaacatactc cagcgccgcg ccaagaccaa taatgccccc
     2401 ggtattgggt gtaccggctt caaaccgcca tggtgctttg gtccaggtag tgccttcact
     2461 caggctgacg gtggcgatca tagaaccgcc cccttcccac ggcggcatct cctgcaacaa
     2521 ggcttctttc acataaagaa tgccaattcc ggtggggcca tacagtttat gcccggagaa
     2581 cacgtaaaag tcgcaatcca gcgcctgaac atccaccgga tgatgcatca ccgcctgagc
     2641 gccatccacc agcacttttg cgccatgctg gtgcgcaagc gtgattattt ccgccagcgg
     2701 attttctgtt cccagcacgt tggagacatg agtaattgcc agcaggcgag ttttctcatc
     2761 aaacagcgta ggcagcgtct ccagttgcag cgtaccgtcg gggttgagag ggatcacacg
     2821 cagctctgcg ccaacgcgtg cgcaaagcat ctgccaggga acaatgttag cgtggtgctc
     2881 catctgactg atgatgatgt tatcgcccgc ccgcacgttg ctgttgcccc aactattggc
     2941 gaccagattg atcccttccg tcgtgccgcg gacgaacacc agctcttccg ccgaacgggc
     3001 attaataaac agcgatgccc gcttgcgcac gttctccatt ttctcggtcg cctgggcgct
     3061 taaggtatga ataccgcgat gcaccgccgc gtagccgtga cgataaaact cagcctcggc
     3121 gtcaatcacc tggcttggtt tctgcgcact ggcggcgcta tcgagataag ccagcggcaa
     3181 accgttcacc tcacgagaaa gcaccggaaa gtcggcccgc actttgtcga cggaaaaagt
     3241 catcttgcac ctcctggcag ccgttgaccg attcgggcca gcacctgctg tttaagcccc
     3301 tcatcacgca gtgcttccgt cagttcggca gcaaaggcgt aaatgatcat ctgctgggca
     3361 tcctgctgat tgatcccgcg cgagcgcaga tagaacatct gttcatcatc aatacgcccc
     3421 accgtcgcgc cgtggctgca tttcacatca tctgcataga tttccagctg cggttttgta
     3481 tccacttccg ccattttgcc catcagcaga ttattgttgg tcatctgacc atccgttttg
     3541 atggcgtgct gcgcgacgtt gatcaaaccg ttaaataccg cgcggccttt gtcgctgacg
     3601 atagttttgt gcaactgtcg gctgttacaa aaacctttat tgtgttccag ccaggtacgg
     3661 gtatcacaca cctcgttttt caccggcatc gccaggctat tgatccgcag cgtgctgttt
     3721 tcgccattga gttgcgtact ggtgttgtgt cgtaacactg cgccacccag caggaaacta
     3781 tggctaaatg cggtggcatc ctcagccagc aacaaatcgt tatgagcaaa gtggtgactg
     3841 agcgggtttt caaacgccag cttgatatgc tgcaagtggg cgttcgctgc gacattgata
     3901 gtgaaccgtg ccccggtaaa atgacgagca tcattaaggc tgacaaaatg ttcgatcacc
     3961 gttgcttcgg caccttccgc cagatccaga tgatgccggt aatgggcagt gttcacctct
     4021 tcacctgcca cgccctgggt gatatgcatt aacagcaatg gctttgccgg ccgttgaccg
     4081 cgcttcacgg cgatatgcgt cacgctttgt gccaggcttt ccgtcaaatg cagaaacact
     4141 tccgcctgaa tagcgtcggg taacccctga cggtcgtcgt taatgctcac ttcatatccg
     4201 ctgccttcaa ttgcatcgct cagcgcgggc acgtaacgtc catcgacaaa caccagccgc
     4261 acggcgtcta acgttaacgc taaggcatca cgctgctgtg gggatatatc tcccgcaatg
     4321 ctgacaaact gactattggt cagcccttcc agcggcgtat atttccagtt ttcatgttta
     4381 cgtgtcggca gtccggtacg cagcaattgt tgtaaatgct gctgtgcttg cggtgagcgt
     4441 tttgtccctt cagcttcaaa caagtgatgc cactgttgca gcgcattact gctgttcggt
     4501 aagccagcca taaccctgct cctccagttg tttgaccaac gtgaaatcgc cggatttcac
     4561 aattcgtccc tgatatagca catggacgta atcaggcttg atgtagtcga gaatgcgttg
     4621 gtagtgcgta acaatgatga atgagcgctt gccgtcacgc agcgagttca cgccatcggc
     4681 aaccactttt aatgcgtcaa tatccagccc ggagtccgat tcatcaagaa tgcataactc
     4741 cggttccagc accgccattt gcaaaatatc gttgcgcttt ttctcgccgc cggaaaaacc
     4801 aacgtttacc gaacgggtta ataaatcttc cggcatcttc aggagagcga ttttctcttc
     4861 catcaaatcc tgaaaatcaa agcggtcgag cgtttcctgg ccgcgatagc tgcgcaccgc
     4921 attaagtgcc gtttgcagga aaaactggtt actgacgccg ggaatctcca ccggatactg
     4981 gaaggccata aagatgcctt cgcctgcacg atcttccggc gacagcgcaa gcaaatcttt
     5041 gcctttgaac tcaaccgtgc cgcccgtcac ttcataatct tctcgcccgg caagcgttgc
     5101 cgataaggta cttttgcccg aaccgtttgg ccccataatg gcgtgaactt cgccgggacg
     5161 aacgtcgagg cttaatccac gcaggatagc tttatcttcc acgctgacgt gtaaatcttt
     5221 aatacttaac atgtttattc cttatccgac gctgtgttca agactgatgg cgaggagttt
     5281 ttgtgcttca acggcaaatt ccagcggcag ttctgagaac acgtctttgc agaaaccgtt
     5341 aacaatcatc gagatggcgt cttcttcgct aatcccgcgt tgcaggcagt aaaacagttg
     5401 atcttcacca atacgtgatg tcgttgcctc gtgctccagt tgtgcgctgt tattgcgaca
     5461 ttcgacatac gggaaggtat gcgccccaca attagcgcca atcagcattg agtcgcactg
     5521 agtgaaattg cgcgcattgg ttgccgtcgg catgattttc actaagccgc gataactgtt
     5581 ctgactatgt ccggcagaga tccctttcga gataatggtc gatttggtgt ttttaccgat
     5641 gtggatcatc ttggtgccgg tatccgcttg ctgatgaccg ctggtcagcg ccactgagta
     5701 aaactcacca atggagttat cgccgcgcaa aatgcagctg ggatatttcc acgtaatcgc
     5761 tgacccggtt tctgattgcg tccatgacat tttgctgttt tcgccttcgc acaaagcacg
     5821 cttggtgacg aaattgagaa taccgccggt gttgttatcg ccagggaacc agttttgtac
     5881 cgtggaatat ttcacctcgg cgtttttatg gatgatgact tccaccaccg ccgcgtgtaa
     5941 ctgatagctg tcacgcaccg gagcggaaca gccttcaatg tagctgacgt agctgtcttc
     6001 gtcggccacc agaatggtgc gctcaaactg cccggttttt tccgcgttaa tgcgaaaata
     6061 ggtggaaagt tccatcgggc agcgcacgcc tttaggcaca taaataaacg taccgtcaga
     6121 ggctaccgcc gcattaagcg cggcaaagaa gttgtcattt cccggcacca cagtgccgag
     6181 atatttacgc accagttccg gatgatcgtg gatcgcctca ccaaaggaac agaaaataat
     6241 tccctgctcc gccagttttt cgcgataagt agtggcaacc gaaacagagt cgaaaatggc
     6301 atccaccgcc acctctttgc cttcccgcac gggaacgccc aactgctcaa acgccgcctc
     6361 cacctcttta cttaaaaagg cgttcgcgcc agtttgctgc accgcgccag gttcagacgc
     6421 gcaagtgtcg tcacaattac cgcacgatgg tgctgagtag taactgtaat cctgataatt
     6481 cagcttgtcg tagtgcgctt tcaaccagtg cggttcttcc atctccagcc atgcgcgata
     6541 ggcgtttaga cgaaactcca gcatccactc cggctcatta cgcttcgccg aaattgcgcg
     6601 caccacctct tcgtttatcc cctttgccag ctcatcggtg gctaactggg tgaagaatcc
     6661 ttctttataa ttcagcgggc cgccggtcca ggttttgaca tcgtcagttg cttcagtatt
     6721 acgagacata gtaccgccta taccccaaag ctttcgccac agccacattc attctgggct
     6781 ttagggttgt gaaatttgaa tatctgatta agtccttcac gaacgaaatc gacttccgtg
     6841 ccatcaataa acggcatcgc ttgcagcggg acaaacagct tcgcgccgtc gtgttcaaac
     6901 agcagatcgt ctttgtccgg ctcgctaaca ctgtcgagca catagccaaa gcccgcgcag
     6961 cccgtttgct tcacgcctaa gcgcacgccg accatacccg gctgctttgc caccagctca
     7021 cggatgtgta tcgccgctgc gggtgtcagc gttaagcctt gccaggcgaa atcttgtggg
     7081 ttaaaggttc ctgaatgcat gtccatcgat ttacctcact tcatcgcttt cagcgtataa
     7141 cagcatgcta gtgataatga ttatcagttc aacccagcaa acgcaggggc tttaccgtaa
     7201 aacatgcttt ttgcctgctt ttaataagca tagaccctga tttgtgggtt aacaggcacg
     7261 ctaagaatta ggtatctcat tgttagataa tggttattat ctaaggagca ttaaggctgt
     7321 gatgaaaaag aaaaagttgt attgaaaatg actatttaag agataggtaa aaaagtgcag
     7381 cgttcagaaa taagaaaacc cttaagtctg tgcgacacag gcttaagggt ttctacccca
     7441 tccggcgctt atctccggca ctctcagtgg cttagctctt gaaggggcga taagaataat
     7501 ctcataaagc taacccgccg ttttaacaca aactgcgatt agtattattt ttgaacaata
     7561 tcaggcggta gataagcagt attaagaagg tcatcgaacc tggacggagg ttaatccagg
     7621 tcgatttggc gaacttgcgg cattaagtca ggatcaatgc acgcccagac gccaggcaaa
     7681 gtagatttct tcttttaatt cagtagaaga gagagtaagc aggtcagcaa attcaagttc
     7741 tagttgtttc agacgtttga gatattgggc aggtgaaaga ttgctctggt cacggcgtaa
     7801 aaattcaatg gccagctggg tgggatcaag ttgagtagac atagcatcct cgcttttaga
     7861 caagacgtgc acagtatacc accgtttact gtgcagataa tgaccaaaag caatatgcgt
     7921 cacacttttc tggtgacaac gtcacaaaat ggcggtcgtc aatcgtgacg aacagcacaa
     7981 acgccctttc tcatcgaaga tttcaatctg ccagacctgg tgacgcgaac cgagatgcaa
     8041 cggtttacat atgccgcgca cccgcccttc tcgtgccgag cggacgtgat tagcattgat
     8101 ttccagacca accacttttt gctcaccttc ggtacataaa taaccggcaa cggaaccgat
     8161 actttcggcc agtaccacgg atgctcctcc atgcagcaac ccgaaaggct gctttgtccg
     8221 cgagtctact ggcattgtcg cttcaagggt gtcatcacca atatgttcaa agcgaatatc
     8281 caggaacccc accatgtttc cttcacccat agcattcagt gcttccaggg tgattttccg
     8341 tttccatatc atttaataat ctccagtaaa gcctgcacag gatggcgtac ccccgtgcct
     8401 tcaacccgtt ttacctggct acggcaggaa tatccggtcg ccagacagcg gttacgcggc
     8461 agtcgctgca tagcctgatg ccaggataac tcatagatcc cgagcgaatt ttcatggttt
     8521 ttcgcttcat gtccgtaagt ccctgccatg ccgcagcaac ccacgctgac attttccagt
     8581 ttcgcgccaa aacgggcaaa tatcgcggcc cattgtgctg gcgcgcccgg caaagcggta
     8641 acctcggtac agtgaccaaa gaaataccat gattcaccgc tgactgtagc cactggatgt
     8701 gactcaagtg cgcttgccag ccattcattc gcaagtaaga cgttaaactc gccacgttcc
     8761 tcgcccagcg ccagtttata ttcatcgcga taacaaagta ccagcgccgg atcgacgccc
     8821 accattggca tcccaagctt agccatacgg ttgaggaaat ccgccgtctt tttcgccgtc
     8881 ttcgcaaaac gattaagaaa gcctttgata tgctgggctt tgccatttgg cgaaaatggc
     8941 agtaacacag gctggaaacc taatttttca accagacgga caaaatccgc caccacttgc
     9001 gcatcgtaat agctggtaaa ggggtcctgc accaccaaca ccgtgcgcgc tttctgctct
     9061 gcattgagag cttcaagctg ttccagcgtc atgtttgccg agcgatgccc caccatttgt
     9121 tgttgtagcg aggggaccga cagcagtggc aaatcaacca tgccgatatg tttttccgag
     9181 agtttgcgca ccagcggctg gttaatgaag aagttaaagg ttttcggcgc gcgtgccatc
     9241 agcggtgcgt agctctcgac cgtagcgacg aggtggtcgc gcagcgggcg taaataacgg
     9301 gtgtgataga gctgcaggaa acgagagcga aactccggca catcaatttt gatggggcac
     9361 tgggtcgaac acgctttaca ggccagacag cccgacatcg cctctttgac ttcgtgtgag
     9421 aagtcatatt cgcctttatt cgcatgccag ctattgcgcg tgcgggcaat taacgtccgt
     9481 aaactgacgc cggattcagg cagttcttgt tccagtttga gtggatcaac gccgcgatcc
     9541 gccaacaaac gcagccattc acgcaccagc gttgcgcgcc ctttcggtga atgaatccgg
     9601 ttctgggtga tcttcatcga cggacacatc ggactacggg catcaaagtt gaagcataaa
     9661 ccgttgccgt tacactccat cgcaccgcgc cactgctggc gtaccgcaat ggggatctgc
     9721 cgatcgaatg taccgcgctt caccgcgtcc actttcatca tcggcgcatc gagaccttct
     9781 ggcgggcaaa tcttccctgg gttgagtcgg ttatgcgggt caaatgccgc tttcactttg
     9841 cgcagttctg caaaaagttc ctcaccgaaa aacgccgggc tgtattcagc gcgaaaacct
     9901 ttgccgtgct cgccccacaa caaaccaccg tatttcgcag tcagcgccac cacgtcatca
     9961 gagatttgct tcatcaaaat ctcttgttgc ggatcgcaca tatccagtgc cggacggacg
    10021 tgcaaaacac ctgcgtcgac gtgaccgaac ataccgtagc ttaagccgtg gctgtcgagc
    10081 agcgcgcgaa attcagcaat ataatcggcc agatgttccg gcggtacgca ggtatcctca
    10141 gcaaacggaa ttggcttagc ggcacctttg gcattgccaa gcagaccaac ggcttttttg
    10201 cgcattgcat agatacgttc aacccccgcc agctcgcggc acacctgcca gccaatcaca
    10261 cctgcctgtt ggctggcaat cagctcatca agccgcacac agagtgcatt aactcgctcg
    10321 tcaatcagcg cctcatcatc accagcaaat tccacaatgt tcagcccgag catctctttg
    10381 tcaggcacat cggtaatcaa ctcgctgacg gaatgccaga caatatcttc ccgcgccaga
    10441 ttcagcactt ttgagtccac cgtctctacc gaaagcgccc gcgcctcaac cataaacggc
    10501 gcgttacgca gcgcggagtc aaaagagtca tatttgacgt tcaccagacg gcgcacttta
    10561 ggcaagggcg taatatccag ccgcgcttcg gtaataaagg ccagcgtccc ttctgaaccc
    10621 gtcagaatgc gcgtcaggtc gaactcggtc atctcatcgt taaagacatg acgcagatcg
    10681 taaccggtaa gaaagcggtt aagtttgggg aagttgtcga tgattaactg gcgttgcaga
    10741 cggcaacgtt gataaaccgt gttataaatt cgcccgattg tggtattggt tttacccagc
    10801 gtttccgcca gttcgacggg taaaggttgc gtatcgagaa tatcgccccc caacaacacc
    10861 gcgcgtacgc caagtacgtg atctgacgtt ttgccataga ccagcgatcc ctgaccggat
    10921 gcatcggtat tgatcatccc gccgagcgtt gcccggttgc tggtcgaaag ttctggtgca
    10981 aaaaagtagc cgaacggttt caggtactga ttgagttgat cttttatcac cccggcctca
    11041 acgcgcaccc agccctcttc agggttaatt tcgatgatgc ggttcatatg gcgggacata
    11101 tcaacaataa tcccctggtt gagcgcctga ccgttagtgc cggtgccgcc gccgcggggg
    11161 gtaaagatca gcgatgaata gcgttcctgc gcggcaagac gggcgatcag cgccacatct
    11221 gcggttgaac gcggaaatac caccgcatcg gggagaagtt ggtaaatact gttgtcggtc
    11281 gacattgtca gacgatcggc ataacttgtc gccgtatcgc cggtaaaacc ttgttgctcc
    11341 agctcttgca aaaaattaag caccagttga acgacgccgg gtgcctggga aatctgtgga
    11401 atcattatat tgaccctttc ctgcggtctg tgatgtaggt cgatacacta ttctttcagg
    11461 cggctgcaat agcgcactga aaggtgatgt ttatttactc tatggatttc gagttgcagg
    11521 aaggcggcaa gcgagtgaac tccaggagct tacaatagta agtgactggg gtgaacgaac
    11581 gtagccgcag cgcatgcaac ttgaaatacg acgagtaaat cgtttgcgtg ttgcctgagt
    11641 tgttgtacca catttttttc taacacgccc atcagaatta agggcagaat cggcctgtta
    11701 aaaaccgctg aaattgctca tcattatgca ggtgagtttc gcgtgttcac gtcgcgtcga
    11761 cgatttgacg cacaaaaaag gtgaaaagta gttatggtaa atgttcgtca gcccagggat
    11821 gtcgcacaaa ttctgctttc ggtgctgttt ttagccatca tgattgtggc atgtctgtgg
    11881 attgttcaac cctttattct cggctttgca tgggccggta cggtggttat cgccacctgg
    11941 ccggtattgt tacgtttgca aaagatcatg tttggccgcc gctctctcgc cgttctggtg
    12001 atgacgctgt tattagtgat ggtgtttatc atccctattg ctttgctggt taacagtatc
    12061 gtcgacggca gcggcccgct aattaaagcc atttccagcg gcgacatgac gttacccgat
    12121 ctggcgtggc ttaataccat tccggtgatt ggcgcgaagc tgtatgcagg ctggcacaac
    12181 ttgctggata tggggggtac ggcgatcatg gcgaaagtcc gcccttatat tggcaccacc
    12241 accacctggt tcgttgggca ggcggcgcat atcgggcgct ttatggtgca ttgtgcgctg
    12301 atgcttctct tcagtgccct gctgtactgg cgcggtgaac aggtggcaca aggcattcgc
    12361 cattttgcaa cccgtctggc aggcgttcgc ggagatgccg ccgtgctgct ggcggcacag
    12421 gctatccgcg cggtggcgct gggtgtggtg gtgacggcgt tagtacaggc agtgcttggc
    12481 agtatcggcc tcgccgtatc cggcgtacct tatgcaacgt tgctaacggt gttaatgatc
    12541 ctctcctgcc ttgtccagct tggcccgttg ccggtactga ttccggcgat tatctggctc
    12601 tactggaccg gcgataccac ctggggaacg gtattgttag tgtggagcgg tgtggttggc
    12661 acgctggata acgtcatccg cccaatgtta attcgcatgg gtgccgattt accgctgatc
    12721 ctgatcctct ctggcgttat tggtggtttg attgctttcg ggatgatcgg tctgtttatt
    12781 ggtccggttc tgttagccgt ttcctggcgt ctgtttgccg cgtgggtgga agaagtcccg
    12841 ccgccgacgg accaaccgga agaaattctc gaagaacttg gcgaaatcga gaaaccgaat
    12901 aagtaatttc tcatcaggcg gctctgccgc ctgattgtta atcaccgcta attgatattt
    12961 gcttgctctt ccccatattt tcctgcttac cgccattcag ct
//
LOCUS       DAZOYL010000099        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZOYL010000099 DAZOYL010000000
VERSION     DAZOYL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.099"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHUOY_002054"
     CDS             <1..191
                     /locus_tag="ACHUOY_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0682834.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHUOY_002055"
     CDS             191..1393
                     /locus_tag="ACHUOY_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR0682835.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUOY_002056"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUOY_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR0682836.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHUOY_002057"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHUOY_002057"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0682837.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUOY_002058"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUOY_002058"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR0682838.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUOY_002059"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUOY_002059"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR0682839.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUOY_002060"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUOY_002060"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR0682840.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUOY_002061"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUOY_002061"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR0682841.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUOY_002062"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUOY_002062"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR0682842.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3577 g         3153 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggcgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc atcaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gattgcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctagtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgcgggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZOYL010000100        12958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZOYL010000100 DAZOYL010000000
VERSION     DAZOYL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.100"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(349..435)
                     /locus_tag="ACHUOY_002063"
     tRNA            complement(349..435)
                     /locus_tag="ACHUOY_002063"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:Leu,seq:gag)
     gene            complement(450..782)
                     /gene="secG"
                     /locus_tag="ACHUOY_002064"
     CDS             complement(450..782)
                     /gene="secG"
                     /locus_tag="ACHUOY_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR0682843.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1010..2347)
                     /gene="glmM"
                     /locus_tag="ACHUOY_002065"
     CDS             complement(1010..2347)
                     /gene="glmM"
                     /locus_tag="ACHUOY_002065"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR0682844.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2340..3188)
                     /gene="folP"
                     /locus_tag="ACHUOY_002066"
     CDS             complement(2340..3188)
                     /gene="folP"
                     /locus_tag="ACHUOY_002066"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR0682845.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3278..5212)
                     /gene="ftsH"
                     /locus_tag="ACHUOY_002067"
     CDS             complement(3278..5212)
                     /gene="ftsH"
                     /locus_tag="ACHUOY_002067"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR0682846.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5312..5941)
                     /gene="rlmE"
                     /locus_tag="ACHUOY_002068"
     CDS             complement(5312..5941)
                     /gene="rlmE"
                     /locus_tag="ACHUOY_002068"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR0682847.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6067..6360
                     /gene="yhbY"
                     /locus_tag="ACHUOY_002069"
     CDS             6067..6360
                     /gene="yhbY"
                     /locus_tag="ACHUOY_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR0682848.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6516..6992)
                     /gene="greA"
                     /locus_tag="ACHUOY_002070"
     CDS             complement(6516..6992)
                     /gene="greA"
                     /locus_tag="ACHUOY_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR0682849.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7240..8673
                     /gene="dacB"
                     /locus_tag="ACHUOY_002071"
     CDS             7240..8673
                     /gene="dacB"
                     /locus_tag="ACHUOY_002071"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0682850.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            8728..8835
                     /locus_tag="ACHUOY_002072"
                     /pseudo
     CDS             8728..8835
                     /locus_tag="ACHUOY_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8859..10031)
                     /gene="cgtA"
                     /locus_tag="ACHUOY_002073"
     CDS             complement(8859..10031)
                     /gene="cgtA"
                     /locus_tag="ACHUOY_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR0682851.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(10047..11012)
                     /locus_tag="ACHUOY_002074"
     CDS             complement(10047..11012)
                     /locus_tag="ACHUOY_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0682852.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11139..11396)
                     /gene="rpmA"
                     /locus_tag="ACHUOY_002075"
     CDS             complement(11139..11396)
                     /gene="rpmA"
                     /locus_tag="ACHUOY_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR0682853.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11417..11728)
                     /gene="rplU"
                     /locus_tag="ACHUOY_002076"
     CDS             complement(11417..11728)
                     /gene="rplU"
                     /locus_tag="ACHUOY_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR0682854.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11987..12958
                     /gene="ispB"
                     /locus_tag="ACHUOY_002077"
     CDS             11987..12958
                     /gene="ispB"
                     /locus_tag="ACHUOY_002077"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR0682855.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
BASE COUNT         3207 a         3580 c         3039 g         3132 t
ORIGIN      
        1 ttctttttaa atgatgcggc cattgcgttc tcagtaatta aataacatta tcaatgcgtc
       61 tctaaacatt acgcagaacg tagccatcag tagttatact gacaggagca ggatggttta
      121 tcgagaagta aacatatccg tattcgtacc ggaactggag ttgacacaat aaagtgccaa
      181 ttatgtcagc agaagggaaa agtttataac taaggcgtac cggcaccatc gtttcaaggt
      241 accagctacg agtaaagcaa ctggacgaga tacagatacc tgacaaccat tcctcagacc
      301 aggaccaaaa cgaaaaaaga cgcttttcag cgtctctttt ctggaatttg gtaccgagga
      361 cgggacttga acccgtaagc cctattgggc actaccacct caaggtagcg tgtctaccaa
      421 ttccaccacc tcggcacgga tactactttt tagttcggga tatcgctggt cggcttagcc
      481 ggagcagctg gctgagtttg ttcggttttc gccggtgcac tcagattttc ccattcgcta
      541 cctttattgg ttttgttgct attgatgtta cccagcacca gactgatgat aaagaataac
      601 gttgccagca gcgccgtcat gcgggtcatg aagttaccag aaccacttga accaaacagc
      661 gtagcggaag cgcctgctcc gaaggaggct cccatatcag cgcctttacc ttgctgcagc
      721 atgatcagac caacaaggcc aattgccaca ataaggaaaa ctaccaaaag agcttcgtac
      781 ataatcaacc tgttccttgc ggagttgccg cgtaccaatg cttcaaccaa taaagcggga
      841 gtttttaatc tttcccactg aagtgggtgt gaatactaac caaagcgaat gaccttcgca
      901 agggcaattt tatcgcattg tatcaactgc ggaaaaaaac agcaaaaccg atgtgttcgc
      961 tgagaaaaaa ggcgacaatc gccgcctttt tagccagtta tttaccgttt taaacggctt
     1021 ttactgcatc ggcgatacgg tgtgcaaatt cagttacctg cgcttcgtct tcaccttcta
     1081 ccatcacgcg aattaacggt tcggtgccgg atttacgcaa cagcacacgt ccacggctac
     1141 ccagcgccgc ttcaacttcg gcggtcacag ctttaactga ctcatgctca agcggatcgc
     1201 cgctacctgc ggtgtaacgc acgttaacca gaatctgcgg gaacattttc atgccgctgc
     1261 aaaggtcgtg caggctcata tggttacgtg ccatcgccgc cagcacctgc aagccagcaa
     1321 cgatgccgtc accggtagtg gttttatcca gcaggatcac atgaccggaa ttctctgcac
     1381 cgatacgcca gcctttctcc tgcatttttt ccagtacgta gcggtcaccc actttcgcgc
     1441 gcgcaaatgg aattcctaac tgtttcaacg ccagctcaag ccccatgtta ctcatcaatg
     1501 tacccacagc accaccacgc agctggccct gacgaagacc ttcacgcgcg atgatataca
     1561 tgatctgatc accatcgact ttattgcctt catggtcaac cataatcacg cgatcgccgt
     1621 cgccgtcgaa ggcaataccg agatccgctt tttcagccag cacacgagcc tggagcgcgc
     1681 gaacgtcggt agcccccact tcggcattga tgtttacacc gttcggctca caaccgatag
     1741 cgataacgtt cgcccccagt tcacgcagca cgttcggcgc gatgtgataa gtcgcaccgt
     1801 ttgcgcaatc caccacaatc ttcagttcgc tgaggctaag ttcgttcggg aacgtggctt
     1861 tgcaaaactc gatatagcga cccgcagcat caacgatacg gctggcttta cccagttctg
     1921 ccgaatcaac acagctgatc tctttttcca tttccgcttc gatggcctct tctaccgcat
     1981 ccggtagttt ggtgccgtcg atagagaaaa atttaatgcc attatcgtag aacgggttat
     2041 gcgatgcaga tatcacaatt ccggcctctg cgcggaaggt acgcgtcaga taagccacgg
     2101 ccggtgttgg catcgggcca gtgaagagtg cggaaaggcc cgctgccgcc agacccgctt
     2161 ccagtgctga ctccagcata tagccagaaa tacgcgtgtc tttaccaata atgatcttac
     2221 gggagccgtg gcgcgccagc actttacccg cggcccaacc cagcttaagc acaaaatcag
     2281 gtgtgatcgg cgcatcccct acacgaccac gaatcccatc ggtaccgaaa tatttacgat
     2341 tactcatagc gtttgttttc ctttgcagac agagtggctt ccaccacccg catcgcttct
     2401 acggtttctt tgacgtcatg aacacgaata atgtgcgcgc cttgcattgc ggcaatgacc
     2461 gcacaggcca gactaccgct caggcgctcg gacggcccca cgttcagcag ctgcccaatc
     2521 atcgattttc gtgacatacc taccaacagc ggcaggttga aatggtgaaa ttcagccagg
     2581 cgcgccagta atgaatagtt atgggagaga tttttaccga aaccgaatcc ggggtcgagc
     2641 aacaatttct cttttgcgat acccgcctgc tcgcaacgtg ctatttgctc aataaagtag
     2701 cgattcactt ctgcaaagac atcgtcatac ttcggagctt cctgcatggt ttttggattt
     2761 ccctgcatat gcatcagaca aaccggtaaa ccggtttctg cagccgcctc cagagcgcca
     2821 ggttcggaaa gggagcggat atcgttaata atgtgagcgc caactttcgc tgactcacgg
     2881 atgacttctg gtttggatgt atctaccgag atccagactt cgaagcgttg agcaattgcc
     2941 tcaaccacag gaataacacg ttgcaactct tcttcaacgc taacttccgc cgcccctggg
     3001 cgcgtggact cgccaccaac gtcaatgatc gtcgcgccag cgttgatcat cagattcgca
     3061 tgtttcaccg catctatcag cgagttatgc gtgccaccat ccgaaaagga atcaggcgtg
     3121 acgttgagga tccccattac gtgaggatgg ctaaggtcca gtgaagtgcc ctgggcaaag
     3181 agtttcatgg tgttatccct ggtatgaatt gataagaaaa aaccccggag cacgccccgg
     3241 ggttttcggt acaaatacag tcatctgatg cgggaactta cttgtcgcct aactgctctg
     3301 acatggtgtt acccgggttc ggcgtacgcg gttcatcaac cggacgagga gcctttggac
     3361 taccattgtc gccagaattg ttagaagcgc ctggttcttc ccagcccgct ggcggacgta
     3421 catcgcgacg tgccatcagg tcatcaatct gcggtgcgtc gatagtctca tatttcatga
     3481 gagcatcttt catcgcatgc agaatatcca tattgtcggt cagaagctga cgcgcacgat
     3541 tatagttacg ctcaatcagt gctttcactt cctggtcgat gatacgtgca gtttcatcgg
     3601 acatatgttt cgctttcgct acgctacggc cgaggaacac ttcaccttct tcttccgcgt
     3661 acagcagtgg acccaatttc tcagagaagc cccactgagt caccatgtta cgcgccaggt
     3721 tggtcgcaac tttaatatcg ttggacgcac cggtagaaac atgttccggc ccgtagatga
     3781 tctcttctgc cagacgacca ccgtacagcg tagaaatctg gctttccagt ttctgacggc
     3841 tggcgctgat tgcgtcgccc tcaggcaaga agaaagtcac acccagcgca cgaccgcgtg
     3901 ggataatcgt cactttgtgc accggatcgt gttccggcac caggcgaccg ataatcgcat
     3961 gacccgcttc gtggtaagcc gtcgattctt tctgcgcttc cgtcatcacc atggagcgac
     4021 gttccgcacc catcatgatt ttatctttcg ctttctcgaa ctcaaccatc gacacaacgc
     4081 gtttgttgcc acgagcagcg aacagtgccg cttcgtttac caggttcgcc aggtcagcac
     4141 cggagaaacc aggagtacca cgggcaatga ttgccgcgtc gatatcgggt gccaatggta
     4201 cgcgacgcat gtgaactttc aggatctgct cacgaccgcg aacatctggc aagccaacca
     4261 caacctgacg gtcgaaacgg ccaggacgca gcagggccgg gtcaagaacg tccggacggt
     4321 tagtcgcggc gataacgatg ataccttcgt taccttcgaa gccatccatc tcaaccagca
     4381 tctggttcag agtctgttca cgttcatcgt gaccaccgcc cagaccagcg ccacgctggc
     4441 ggcctacggc gtcgatttca tcgataaaga tgatgcacgg tgccgctttc ttcgcctgtt
     4501 cgaacatgtc acgaacacgg gatgcaccca caccgacgaa catttctacg aagtcagaac
     4561 cggagatagt aaagaacgga actttcgctt cgcctgcaat cgctttcgcc agcagcgttt
     4621 taccggtacc cggaggaccg accatcagga cgcctttcgg gatcttaccg ccgagtttct
     4681 ggaagcggct cggctcgcgc agatattcaa ccagttcagc aacttcttct tttgcttcgt
     4741 cgcagcccgc aacgtcagca aaggtcgttt tgatctgatc ttccgtcagc atgcgcgctt
     4801 tgctcttacc aaacgacatg gcacctttgc caccgccgcc ctgcatttga cgcatgaaga
     4861 agatccacac accaatcagc aacagcatcg ggaaccagga gatgaagata gaagccagca
     4921 ggcttggttc ttcaggcggt tcaccgacaa ccttgacgtt cttggtcaac aggttatcca
     4981 gtaatttcgg atcctgaacc ggaatgtaag tggtataacg gttactatct ttcttggtaa
     5041 cgttgatttc acgtccgttg atacgcgctt cacgaacctg gtcgttattg acctcttgta
     5101 ggaaggtaga gtaatccacc ttacggccat tagactcgct gggcccaaag ctctggaata
     5161 ctgacatcag cacaacggca atgaccagcc agagtattag gtttttcgcc atgtcactca
     5221 agggattaac ctcttattac aactgtgtta aaaacagcgt caggatactc tatatccagc
     5281 atctttcaaa ctttcgtctg aaatctcccg gttagggttt acgcccggtc gctacaatat
     5341 acacttcccg cgaacgtgca cgagaagagt ccggcttacg aactttgacc ttcgtaaaca
     5401 gggagcgaat ttcccttaga tactcatcga aaccttcgcc ctggaacacc ttcactacaa
     5461 aactgccacc tggcgctaat acatcacgac acatttctag cgccagttcc accagataca
     5521 tggcacgggg gatatccacc gccggtgttc cgctcatgtt tggtgccata tcggacatga
     5581 caacctggac tttgctgtcg ccaacgcgct ccagcagtgc tttcatcacc agttcatcac
     5641 gaaaatcgcc ctgaagaaag tctacaccaa cgataggatc cataggtaaa agatcgcaag
     5701 cgatgatgcg gcctttgccg ccaatttggg tgaccacata ttgtgaccaa ccacccggag
     5761 cagcaccaag gtcgacaacc gtcattcccg gtttaaagag tttgtcactt tgctgtattt
     5821 catcaagttt aaaccaggca cgggaacgta accccttttt ctgtgcctgt tgaacatatt
     5881 tatcgctaaa gtgttcctga agccagcggc tggagctggc agaacgcttc ttacctgtca
     5941 tttaactttc ccatggggat aactcatcgt aaccaattgc gtaaattttt acgcgcctat
     6001 ttggtgatat atgggagatg gcggtagaat gacccatttt caatcccaac gtaagcaaaa
     6061 aatacgatga atctgagtac taaacaaaaa cagcacctga aaggtctggc acatccgctc
     6121 aagccagttg ttctgcttgg cagtaatggt ttgaccgaag gggtgctggc cgagattgaa
     6181 caagcgttag agcaccatga actcatcaag gtgaaaatcg ccaccgaaga tcgcgaaaca
     6241 aaaaccttga tcgtggaagc tatcgtgcgc gaaaccggcg cctgtaatgt acaggtcatc
     6301 ggtaaaacgc tggtgcttta tcgcccaact aaagaacgta aaatctcgct gccacgctaa
     6361 gattatccta aagttacaca catttgctgt gtaaaacgag gggttttccg caggcaggag
     6421 agcaaaatgc cacgctctgt tcgttgataa aaggccgcat agcggccttt ttcctttctt
     6481 tacaatacat caacatcttg agtattgggt aattcttaca ggtattccac cttaattact
     6541 tcaaattcta cttcgccgcc cggcgttttg atgaccacaa catcatcttc ttctttgccg
     6601 atcaggccac gagcaatagg cgagtttaca gaaatcaggt tttgtttaaa gtcagcttcg
     6661 tcatcgccaa cgatgcgata agtctgttct tcgtcagaat ccagattcag caccgttacg
     6721 gtagcaccaa aaataacgcg cccattgtta ggcattttgg tgacatcaat cacctgcgcg
     6781 tttgacagct tggcttcgat gtctttgata cggccttcgc agaagccctg ctgttcacga
     6841 gctgcgtggt attcggcgtt ttctttcagg tcgccatgct cacgcgcttc cgcgatagca
     6901 gcaatgattt caggacggcg cacagatttc agaaaatcca gctcttcgcg taatttttca
     6961 gcgccgcgta aggtcatcgg aatagcttgc atttgttata cctcttgaat attcctgatg
     7021 gggcaagtct tcacccaccc cggctgttcg gcctgcccgg cataactcct caccgggacc
     7081 agaagcaaaa aaataccgac ccaggtacaa gtcccaggtc agctacaatt cacattttga
     7141 tagtcatttt accctgaagt tcccgaaggg tcatcgttta ctttataggg cgttgcgccg
     7201 tagtatgacg gctcgattcc aggttgttag cgcgagatta tgcgattttc cagatttatc
     7261 atcggattga ccagctgtat agcgttcagt gttcaggccg caaatgttga tgagtacatt
     7321 actcaactcc ccgctggtgc caaccttgcc ctgatggtgc aaaaagtcgg cgcgtcggcc
     7381 cccgctattg attaccacag tcagcagatg gcgctgcctg ccagtaccca gaaagtgatt
     7441 actgcgctgg cggcgttgat tcaactcggc cccgattttc gttttaccac gacgcttgaa
     7501 accaaaggca atgtggaaaa cggcgtactt aagggtgact tagtggcgcg atttggtgcc
     7561 gatccgacgt taaaacgtca ggatattcgc aatatggtcg cgactttgaa aaaatctggc
     7621 gtcaaccaaa tcgatggcaa tgtgttgata gatacctcca ttttcgccag ccacgataaa
     7681 gcccccggct ggccatggaa tgacatgaca caatgcttta gcgctccgcc tgccgccgcc
     7741 atagttgacc gcaactgttt ctccgtctcg ctctacagtg cccaaaaacc cggtgatatg
     7801 gcctttatac gcgtggcatc ttattacccc gttacgatgt tcagccaggt acgcaccctc
     7861 ccccgtggtt ctgccgaagc gcaatactgc gaactggatg tcgtaccagg cgacctgaac
     7921 cgctttacgc tgacgggatg cctgccacaa cgttctgagc cgctcccgtt ggcttttgcc
     7981 gtgcaggatg gagccagtta tgccggtgca attctgaaag atgagttaaa acaggcgggt
     8041 atcacctgga gcggaacact gctgcgccag actcaggtta acgaacctgg aacggtagtt
     8101 gccagtaaac agtcggcccc gctgcacgat ctgcttaaga ttatgctgaa aaagtcggat
     8161 aacatgatcg ccgatacggt tttccgcatg ataggccatg cgcgcttcaa tgtacctggt
     8221 acatggcggg ccgggtcgga cgctgtgcgt cagatcctgc gccagcaagc cggtgtcgat
     8281 attggaaaca ccattattgc cgatggttca gggctttcgc ggcataacct gattgccccc
     8341 gccaccatga tgcaggtgct gcaatacatt gcccaacacg acaatgaact taactttatc
     8401 tccatgctgc cgctggcggg ctatgacggc tctttgcagt accgtgcagg tctgcatcag
     8461 gcgggcgtgg atggaaaagt ctcagcgaaa accggttcgt tgcagggggt atataacctg
     8521 gcgggattca ttaccacagc gagcgggcaa cgaatggcgt ttgtgcaata tctttctggc
     8581 tatgcagtag aacctgcgga tcagcgtaat cgccgtattc cgttagtgcg ttttgaaagc
     8641 cgtttgtata aagatattta tcagaacaat tagtcaaaaa gaaaccccgg cacatggccg
     8701 gggcttcaga ttattgacaa agtgcgcttt gtttatgccg gatgcggcgt aaacgcctta
     8761 tccggcctac aaaatcgtgc aaattcaaca tattgcaatt ctcttgtagg cctgataagc
     8821 gtagcgcatc aggctgattt ggcgtttatc atcagtgatt aacgcttgta aatgaactca
     8881 acgccttctt cgtcgtcttc gtcccagtcg tcatcccagt cttcatcatc ctcttcagca
     8941 atctcttcaa gctgctggcg atgataatcg tcccacatga attcgacttt ctctggctgt
     9001 ttcgcttctt cagcctgcac gaccgggttt tcaaggataa aggtcatcac atcccagcag
     9061 agatctttca cgcccagtcc gctcgccgca gagatcagat aatatttatc ttcccagccc
     9121 agcgcttcag caatcgcttt cgctttctct tcagcttctg ccttatccag cagatcgatc
     9181 ttattgaaca ctaaccaacg cggtttcgcc gccagatcct ggctgtattt ttccagctcg
     9241 ctgataataa tacgcgcgtt ttcaaccgga tcggtgccgt caatcggatc gatatcgatg
     9301 aggtgcaaca ggacgcggca acgttccagg tgcttcagga agcggatacc cagacctgcg
     9361 ccttccgcag cgccttcaat cagtcctgga atatcggcaa caacgaagct cttttcgttg
     9421 tccattcgta ccacacccag acttggcacc agagtggtaa acggataatc cgccactttc
     9481 ggtttagccg ccgataccgc gcgaataaag gtcgatttac ccgcgtttgg catccccaac
     9541 ataccgacgt cagccagcag catcagctcc agcagcagct cacgcttatc acccggcgta
     9601 ccgttggttt tctgccgtgg tgtacggtta acggacgatt tgaaacgggt attgcccaga
     9661 ccgtgccagc cgcccttagc aaccagcaga cgctgaccgt gtttagtcat atcgcccatg
     9721 gtttcaccag taccctggtc gattacacgc gtacctaccg gcactttaat cgtcacgtct
     9781 ttaccgcgct taccggtaca atcgcggctt gcgccgttct gaccgcgctc tgcacggaaa
     9841 gatttttcaa aacgataatc gataagcgtg ttcaggtttt cgtcggcttc catccatacg
     9901 tcaccaccat caccaccgtc gccgccatcc gggccgcctt tcggaatata cttttcgcgg
     9961 cggaagctca cgcaaccatt accgccatca cctgcaacga ccagaatcga tgcttcatca
    10021 acaaacttca ttttattctc cataaatcat tcgcctgcgc ggggttgcga taccaccgtt
    10081 gtatgcttac gtaatccgcc ccaaatacga tgaccaatgg cggaatacat cgcgcccgca
    10141 accacgacaa acgcaccgag ataacctaaa aggtttaaca tcggtctggc gaagaaatcg
    10201 ggccaggcca gtgataaaag atctgaaaaa aacagcgtaa acagtggggt gagcgtgatg
    10261 atcgcgctca cctgcgctgc ctgccagcga gccatcgctt ccgccagggc gccatatcct
    10321 accaatgtat tcagtccgca aaaaattaaa catgcgagct gccagtggct aagctgcgct
    10381 atcactccag gctttgccag agggaagagc gcaattgtac ataaagtgta cagtaaaaac
    10441 aggatctgcg gtgaggccag ccgacgcaat aaaacctttt gcgccacgcc ataactcacc
    10501 caaaccgtcg ccgcaccgac cccaaagata actccccagg tgtaatcggt gagctttgta
    10561 aatatctcga ccagactggt gttaaaaaac atcaccaggc cgctcaggag catcaatgcc
    10621 cctacaacct gagtgctgcg cattttctct ttcaggataa atacgctggc aaccatcatg
    10681 ccaactggcg agagttgccc aatcacctgc gaagcggtcg gactcaggta ttgcaaggat
    10741 gagctgaaca ggatgaagtt cccaaacagc ccggcggtcg ccactgccaa caaaatcaac
    10801 cagcgtggct tacgaaacac gcgtaatggc ggcaacctct tcttcaccgc aagaatggca
    10861 ccaaggccaa tactcgccat caagaaacgg taaaacacga ttgtcggagg ttccatcacc
    10921 tccagcacct gcttcattgc gattggcaac gccccccagc aaattgctgt ggtgagcgcc
    10981 aaaagaatgc caatgcctgc ctgctgcttc ataccgtatt ccctgcaaag cgcatttgcg
    11041 gattaacgtc gcaccatgcg catttttacc ggttatcgaa tgtaaaaagc cccgcaacgt
    11101 gttgcggggc tttcatccgt taccgggacg cgaaaaactt attcagcttc gatgctgata
    11161 aatttacggt ttttcgggcc tttaacttcg aatttcactt taccgtctgc tttagcaaac
    11221 agagtgtggt cacgaccgca acctacgtta gcgccagcgt ggaatttggt accacgttga
    11281 cgaacgatga tgctacccgc cagaacggat tcgccaccga aacgcttaac gcccaggcgt
    11341 ttagcttctg aatcgcgacc gttacgtgtg gagccgccag cctttttatg tgccatttga
    11401 aatctctcct caggtcttag gcgctgatgc cagtaatttt cacatcagtg aaccactgac
    11461 gatgaccctg ctgcttacga tagtgtttac gacgacgaaa cttaacgatt ttaactttct
    11521 cgccacgacc gtgagcaaca acttcagctt tgattacgcc gccatcaacg aaaggaacgc
    11581 cgattttgac ttcttcaccg tttgcgatca tcagcacttc agcgaactca acagtttcgc
    11641 cagttgcgat gtccagcttt tccaggcgaa cggtctgacc ttcgcttact cggtgttgtt
    11701 taccaccact ttggaaaacc gcgtacatat aaaactccgc ttccgcgcac accttttcaa
    11761 tgattcagag tgcgctataa atattcacaa tagggcgcga atattacgca aaacgcacgc
    11821 ctttgacaag tgctacagtc aatacacgaa gaaaaaaaac acaacttgta cggtaacgtt
    11881 tatctgtgcc attttttcag tacaatcacc ctatattcct aaccataaac cctaagttgc
    11941 ctttgttcac agtaaggtaa tcggggcgaa aagcccggct tttgcgatga atttagaaaa
    12001 aatcaatgag ttaaccgcgc aagatatggc gggtgttaat gcggcaatcc ttgagcagct
    12061 taattccgac gtccaactga tcaatcagtt aggctattac atcgtcagcg gcggcggtaa
    12121 acgtattcgt ccgatgattg ctgtactggc tgcacgagct gttggctatg agggaaatgc
    12181 gcatgtcacc attgctgccc tgatcgagtt tatccacacg gcgactctgc tacacgacga
    12241 cgttgtggat gaatcagata tgcgcagggg taaagctacc gccaacgccg catttggcaa
    12301 tgccgccagc gtgctggtag gcgattttat ttatacccgc gcgttccaga tgatgaccag
    12361 cctcggttca ctcaaagtgc tggaagtcat gtcagaagct gtaaacgtca tcgcagaagg
    12421 tgaagttctg caactgatga acgttaacga tccggacatc actgaagaaa actacatgcg
    12481 cgttatctat agcaaaaccg cgcgtctgtt tgaggctgcc gcgcagtgtt ccgggattct
    12541 ggctggctgt acgccggagg aggagaaagg cctgcaggat tatgggcgct atctcggcac
    12601 tgctttccag ttgatcgacg atttactcga ttacaatgcc gatggcgaac agttaggtaa
    12661 aaatgtcggc gacgatctga acgaaggtaa accgacgctg ccgctgctgc atgcgatgca
    12721 tcatggcaca ccagaacagg cacagatgat ccgtaccgcc atcgaacagg gtaacggtcg
    12781 ccatcttctg gaaccggttc tggaagcaat gaacgcttgt ggatctcttg aatggacgcg
    12841 tcagcgtgcc gaggaagaag cagacaaagc catcgcagca ttacaggtgc tcccggacac
    12901 cccttggcga gaagcactca tcggcctcgc gcacatcgct gttcaacgcg atcgttaa
//
LOCUS       DAZOYL010000101        12834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZOYL010000101 DAZOYL010000000
VERSION     DAZOYL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.101"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..477
                     /locus_tag="ACHUOY_002078"
     CDS             <1..477
                     /locus_tag="ACHUOY_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR0682856.1"
                     /translation="AACQGMPGLCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTE
                     PLTAVFADWYQQPVFASLNDDQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLS
                     ARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            492..1349
                     /gene="menB"
                     /locus_tag="ACHUOY_002079"
     CDS             492..1349
                     /gene="menB"
                     /locus_tag="ACHUOY_002079"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR0682857.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1349..2311
                     /gene="menC"
                     /locus_tag="ACHUOY_002080"
     CDS             1349..2311
                     /gene="menC"
                     /locus_tag="ACHUOY_002080"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR0682858.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            2308..3729
                     /gene="menE"
                     /locus_tag="ACHUOY_002081"
     CDS             2308..3729
                     /gene="menE"
                     /locus_tag="ACHUOY_002081"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR0682859.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            3773..4039
                     /gene="pmrD"
                     /locus_tag="ACHUOY_002082"
     CDS             3773..4039
                     /gene="pmrD"
                     /locus_tag="ACHUOY_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR0682860.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(4033..4419)
                     /gene="arnF"
                     /locus_tag="ACHUOY_002083"
     CDS             complement(4033..4419)
                     /gene="arnF"
                     /locus_tag="ACHUOY_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR0682861.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4419..4754)
                     /gene="arnE"
                     /locus_tag="ACHUOY_002084"
     CDS             complement(4419..4754)
                     /gene="arnE"
                     /locus_tag="ACHUOY_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR0682862.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4751..6403)
                     /gene="arnT"
                     /locus_tag="ACHUOY_002085"
     CDS             complement(4751..6403)
                     /gene="arnT"
                     /locus_tag="ACHUOY_002085"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR0682863.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6403..7293)
                     /gene="arnD"
                     /locus_tag="ACHUOY_002086"
     CDS             complement(6403..7293)
                     /gene="arnD"
                     /locus_tag="ACHUOY_002086"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR0682864.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7290..9272)
                     /gene="arnA"
                     /locus_tag="ACHUOY_002087"
     CDS             complement(7290..9272)
                     /gene="arnA"
                     /locus_tag="ACHUOY_002087"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR0682865.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9272..10240)
                     /gene="arnC"
                     /locus_tag="ACHUOY_002088"
     CDS             complement(9272..10240)
                     /gene="arnC"
                     /locus_tag="ACHUOY_002088"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR0682866.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10244..11383)
                     /gene="arnB"
                     /locus_tag="ACHUOY_002089"
     CDS             complement(10244..11383)
                     /gene="arnB"
                     /locus_tag="ACHUOY_002089"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR0682867.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            11691..12293
                     /gene="ais"
                     /locus_tag="ACHUOY_002090"
     CDS             11691..12293
                     /gene="ais"
                     /locus_tag="ACHUOY_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR0682868.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(12332..12757)
                     /gene="nudI"
                     /locus_tag="ACHUOY_002091"
     CDS             complement(12332..12757)
                     /gene="nudI"
                     /locus_tag="ACHUOY_002091"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR0682869.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT         3173 a         3256 c         3364 g         3041 t
ORIGIN      
        1 gcggcttgcc aggggatgcc ggggctttgt ggggttgttg tcgaaggcgg gcatcctggg
       61 ttgcaaaatg ctgaacaacg cgcggaacgt cagcgttccg atcgccaatg ggcgcagcgt
      121 tttcgcacag aaccgttaac ggcggtattt gccgactggt atcaacagcc tgtttttgcc
      181 tcgctcaatg acgatcaacg ccgggagctg gtggcgctgc gcagtaacaa taatggcgca
      241 acccttgccg ccatgctgga ggcgacttct ctcgccgtcc agcctgattt acgtgctaac
      301 cttagcgccc gcacatttgc gttttattat ttatgtggtg aacgtgacag caaattccgc
      361 gccttggcgg cggaactggc tgccgactgc catgtcattc ctcgcgccgg acataacgcg
      421 catcgggaaa atcccgctgg cgttatcgca agtctggcgc agatcttgcg tttctgacta
      481 aaggacacaa tatgatttat cctgatgaag caatgcttta cgcaccggtt gaatggcacg
      541 actgctccga aggttttgag gacattcgtt atgaaaaatc caccgacggt atcgcaaaaa
      601 tcaccattaa tcgtccgcag gtgcgcaatg ccttccgtcc tctgacggta aaagagatga
      661 tccaggcact ggcagatgcg cgttatgacg acaacgtcgg cgtgatcatt ctgactggcg
      721 caggcgataa agcgttctgc tccggtggtg accagaaagt gcgtggtgat tacggcggct
      781 ataaagatga ttccggcgta catcacctga acgtgctgga cttccagcgt cagatccgta
      841 cctgtccgaa accggttgtc gcgatggtgg ctggctactc catcggcggt ggtcacgttc
      901 tgcacatgat gtgcgacctg actatcgcgg cagataatgc catcttcggt cagactggcc
      961 cgaaagtcgg ttccttcgac ggcggctggg gcgcttccta catggctcgc atcgtcggac
     1021 agaaaaaagc gcgtgaaatc tggttcctgt gccgtcagta cgatgcaaaa caggcgctgg
     1081 atatgggcct tgtcaacacc gtggtaccgc tggcggatct ggaaaaagaa accgtccgtt
     1141 ggtgccgcga aatgctgcaa aacagcccga tggcgctgcg ctgcctgaaa gctgcgctga
     1201 acgccgactg tgacggtcag gcgggtctgc aggagctggc gggcaacgcc accatgctgt
     1261 tctacatgac ggaagaaggt caggaaggtc gcaacgcttt caaccagaaa cgtcagcctg
     1321 atttcagcaa atttaaacgg aatccgtaat gcgtagcgcg caggtatacc gctggcagat
     1381 ccccatggac gcggggatgg ttctgcgcga caggcggtta aaaacccgcg acgggctgta
     1441 tgtttgcctg cgtgaaggcg agcgcgaagg gtggggggag atctccccac tgccgggctt
     1501 cagtcaggaa acctgggaag aggcgcaaat tgtgctgctt gcttgggtaa ataactggct
     1561 ggcaggcgat ggcgagctac cgcagatgcc ttccgttgcc tttggcgtaa gctgtgcatt
     1621 ggcggagctg gcagatacgc tcccgcaggc ggccaattac cgtgcggcac cactgtgtaa
     1681 tggcgatcct gacgatctga tcctcaaact tgctgatatg ccaggcgaga aagtggcgaa
     1741 ggtcaaagtg ggattgtacg aagccgtgcg cgacggcatg gtggttaatc tgttgctgga
     1801 ggcaattccg gatctgcatt tgcgtcttga cgcaaatcgc gcctggacac cgctgaaagg
     1861 tcagcagttt gccaaatatg ttaacccgga ttatcgccac cgcatcgcgt ttctcgaaga
     1921 gccgtgcaaa acccgcgatg attcgcgagc gtttgcccgt gaaaccggca ttgccattgc
     1981 ctgggatgaa agcctgcgcg agccggattt tgcctttgtg gctgaagagg gcgtgcgcgc
     2041 ggtagttatc aaacccacgc tcacgggcag tctggaaaaa gtacgcgagc aggtacaggc
     2101 ggcgcatgcg ctggggctga cggcggtgat cagttcttcc attgagtcga gcttaggtct
     2161 aacgcaactg gcgcggattg ctgcctggtt aacgccagac accattccgg gactggacac
     2221 gctggatctg atgcaggcgc agcaggttcg tcgctggccg gggagcccac tgccgttagt
     2281 ggacgctgat gtactggagc agttgttatg atcttctctg actggccgtg gcgtcactgg
     2341 cggcaagtgc ggggagaagc catcgcctta cgtcttaatg acgagcaact caactggcgc
     2401 gagctttgtg ctcgcgtcga tgagttagct acgggctttg cggcgcaggg tgtggttgag
     2461 ggcagcggcg tgatgttgcg ggcgtggaat acgccgcaaa cgctgctcgc ctggctggcg
     2521 ttactgcaat gcggggcgcg ggtgttgccc gtgaaccctc agctgccgca accgttgctt
     2581 gaagaattgc tgcccaatct gacgctgcaa tttgcgctgg tgccggatgg ggaaaacacg
     2641 tttccggcat taacgtcgct gcacattcag cgggttgaag gcgcacatgc cgctacgtgg
     2701 cagccgacgc gtctgtgctc aatgacgttg acctcgggtt ctaccggttt gccgaaagcc
     2761 gctgtccata cttatcaggc ccatcttgcc agtgcgcaag gtgtgttatc gctgattccg
     2821 tttggcgatc acgatgactg gttgctctct ttaccgctgt ttcacgtctc cggtcaggga
     2881 attatgtggc gctggttata cgctggtgcg cggatgacgg tgcgtgataa acagccattg
     2941 gagcaaatgc tggcaggctg tactcacgct tcactggtgc caacacaact ctggcgtttg
     3001 ctggttaacc gcagttccgt ttccctgaaa gcggtgttac ttggcggcgc ggctatcccg
     3061 gtcgagttga cggaacaggc gcgcgagcag gggattcgtt gcttttgcgg ctatggtctg
     3121 accgagtttg cctccacggt gtgtgcgaaa gaagccgacg gcctggcaga cgttggttcg
     3181 ccgctgccgg gtcgggaagt gaaaatcgtt aataatgaag tgtggctgcg ggctgccagt
     3241 atggcagaag gttactggcg taacgggcaa ctggtttcac tggttaatga cgaaggctgg
     3301 tacgctacgc gcgatcgcgg tgagatgcat aatggcaagc tgaccattgt cggacgttta
     3361 gacaatctgt tcttcagtgg cggagagggt attcagccgg aagaagtcga gcgcgtaatt
     3421 gctgcacatc ctgcggtttt gcaggtgttt atcgtccccg ttgccgacaa ggagtttggt
     3481 catcgaccgg tagcggtggt ggagtatgac cagcaaaccg ttgatcttgg cgaatgggtg
     3541 aaagataagc tggctcgttt tcaacaaccg gtgcgctggc taactttacc gccggagctg
     3601 aaaaacggcg gtattaaaat ttcacgtcag gcgttaaaag agtgggtgca acgccaggat
     3661 tacgaaatca ttagtttcac ttcgcaattt tgccattcca ttgccgttga taataggttt
     3721 atctgttaat ctgtaaattg atgtgaaaac tcttagcaaa caggataatg caatggaatg
     3781 gctggtcaaa aaatcgtgct gcaataaaca agataacaga catgtactta tgctctgcga
     3841 tgctggcggc gcgataaaaa tgatcgccga agtgaagagt gacttcgccg tgaaagtggg
     3901 ggatttactc tcgcctttgc agaatgcgct ttattgtatt aatcgtgaaa agctgcatac
     3961 ggtgaaagtc ctctcggcaa gcagttatag ccccgatgag tgggaacggc agtgtaaagt
     4021 ggcagggaaa actcagtaac gttgttttgt cgtgggcagg aaaatcagca tcaacccgct
     4081 cataatacag gctactccca gtagtgcttt cagcgaaaaa gtcccttccc agccgggaag
     4141 taccattgaa gcaatccata ccagcacata actcatactg agcaaggcat aggctttgct
     4201 tagcgcaagt ttatgtagcg ttttatacca acaaaaaacg gaaagcagat aacccagcag
     4261 cccgagtaac agtatccggg catccaggcc aaaagctaac agagccgcga taaaatccca
     4321 taagtgcgtc atcggcggca gatgactcgc cgcaaaaccc aggcttaatt gcgcaactga
     4381 ggcaataatg acgctgaata atccccacat caggcccatt acaccgtact cccgaggatc
     4441 acaatgccgc caataatgaa cgccacccca caccagtgac gcggcgatac cggttcgtgc
     4501 cacagtttta ctgcagccag cgtcacccag acaaaattca gacttaacat cgggtaggca
     4561 atgcctaccg gtacgttctg caagaccagc agccagagca ccatggcaag accaagacaa
     4621 gccagcgcca gtcccagcca cagcacgata tgtttgcgcc gtttgtttat cgccacaaag
     4681 caggttgcct gtttctgaca caactgcccg gcaacgctaa gcaagctggc aaagactaat
     4741 gttagccaga tcatttggga cgatactgaa tcagcaccag acgctcctga cgatcgatgg
     4801 catctgcggg cggaatggcg agactgttga tatcttcatc gcggtcaacc gaaagcacga
     4861 gtgtaataat cccctcctga cgatgttgat taagccagtt tgcgaactca tcaccgctga
     4921 caaatctccc tttcgcatcc ggataattaa ggccgtattt caactcacct gtctggcgat
     4981 acatgatgat gtcatcgcgt tgcaggctcc atgccagacc tgcggcaaca ccgacgctat
     5041 cagtaagaat atagcggctt ggctgaagtg attcttgtgt catctcgaca aaaaattgcg
     5101 gatgttttcc ttccatgact ctgtcaggaa ttgaaaatcc taccagcaac gccagcccca
     5161 gcgggcaaag cgcggcaaaa gaccaggtct tttcgacgtt tgttaaggtg taccagccga
     5221 aaaatgccca tagcgaaaaa atcgaccagg cacaaaagac tttatagctc tcgaaggttt
     5281 gccacaccgg cgtgttcatt ggtccccacg gggagaccac aaatgtggca ataatgccag
     5341 tgaagccaaa agcgatgtta atccagccat taatccgcag cgccagagga ttattttttg
     5401 ctgccagcaa agcgtaatgc gccatcagca ttgccagaga tgcaaagcag gaaagaatat
     5461 aggtgggcaa tttaccttta gcgacggaga aaaacagcag cggcattatc gtccagctca
     5521 acaaatagac ggttgcggaa tgcttgcggt ttttccagcc tgtgtacagt gcaccgggga
     5581 gtaatcccag ccacggcagg ctaccggcaa tgatgaccgg cacgtagtac cagaacggag
     5641 ctctatgttg ggcatcatcc agtgcaaagc gttgaatatg ctcaacccag aaaaaatagt
     5701 gccagaagtc aggctcccgc tgcgctatcg ccagtcccca ggggagaacc gtcagtacgc
     5761 aactgataac cgccagccag ccgtaaataa agagatcttt ccagcgtttt tgcgtcgcta
     5821 cccatggcag cacgcttaat accggcacgg caagggcgag aaaacctttg gtcatcaccc
     5881 ccatgccgca ggtgattccc agcagtaaaa atcctgcgct tttgcctttc cacgtctgtg
     5941 cctgcattgc cagccagaag ctgcacattc ccgccaccag ccagaatgca ataaacggat
     6001 cgagcacggc ataagtgccg atggcataga caataaacaa tgagagataa attactgtgg
     6061 cgagtagagc cagacgttta tcgcgccata agcgcagagt aaaccaggtc accagcgcgg
     6121 cagttaacag ggtcgcaaag ataacgcctg cccgcacacc aaagttattc gcgccaaata
     6181 gccattgccc aatgctgtta atccagtatc cggcaatggg tttttcgaaa taacgtagcc
     6241 ctaacagatg gggcacaatc cagtcgccgg atgccagcat ttctcgactg atttccgcat
     6301 aacgcgtttc atcgggttgc cagagcagac gcgtgctgat cggtaacagg taatagcagg
     6361 caataaacgc gaagaggccg ataaggtaac gtaccgattt catcagcgac tacccgcaat
     6421 ttgttggcaa cccagccagc cttcacgtcc ggcaatattt ccgcgaacaa cttgtccgag
     6481 cggcaacgtc tctgataaca gttcgcttaa agggcaaaat gtcacgcctt cctgagctgc
     6541 gcgtttgagg agatccacaa aattatgctg ataagcgcag ccttcaactt ctgcatgaat
     6601 ggtataaacc ggcgtgcctt tatctcgcag gatgcggttg agtaaccaac cgttaaaatc
     6661 ttctgctttc acatcccgac caatcacttc atcccaggtg ggtaaggtca ccggaatttg
     6721 cgcagtgcca gggtttcctg attcgagcag tggacggaac ggcatggccc cacgacaatc
     6781 gctgttgtag cgcaaatgga acgcttcttt tgcttcgatc accttctggt cggcacgcca
     6841 gcccgcagcg gcagaacagg ttaccggttg accgataatc tcttccagag tgcgaagacc
     6901 gcgtgcaata tcgtcgatca ttgtttgccg atcccagtta ccgctacggg cttgccaggc
     6961 atggtgatcc caggcgtgca ggccgacttc gtgatgttta gccgcttcac gaatgatatc
     7021 ggcattggca tgaccaatct ctttacctgg ccaggccgta cctgccagta aaatatccca
     7081 gccataaagc gatgccgcgt ttgagcgcag catcttccac aaaaactgtg gcttcaccag
     7141 tcgccagaga tggcggccca tattgtccgg gccgacgctg aaaaaaatgc tggcctgaat
     7201 attatgctta ctcaagattt ccagcagacg tggcacgcct tcacgggtgc cacgaaaggt
     7261 atcgacatca atgcgtaagc cgactttggt catgatggtt tatccgtaag atcaacggtg
     7321 cgcaggaaga aatccagcgt ttcgtcgatg gtttcctgca tatcaatttt cggttcccag
     7381 tccaggcagc ggcgggcatt gcggatgctc ggtttacgat gctctacgtc ctgatatcct
     7441 ttgccgtagt agctgctact ttcgacaaca cgaaagcccg caaacggcgg gaagtgatgg
     7501 cgcagcggat gtttttcgaa gctcgccagc agcatctcgc ccagttcctc aatgctcgct
     7561 tcgttctcag gattgccaat gttgataatt tcgccgtcgc agcgatttcc cgcattttcg
     7621 ataatgcggt acaacgcctc gataccgtcc cggatatcag taaagcagcg tttttgtttt
     7681 ccgccatcaa ttagcttaat tggtgaacct tctaccagat tgagaatgag ttgcgtaata
     7741 gcgcgggagc tgccaattcg cgctgcatta aggttatcca gtcgtggtcc catccagtta
     7801 aacgggcgga agagggtgaa ctgtaaaccc tctttttcgc cataggccca gatcacccga
     7861 tcaagtaatt gttttgacac cgaataaatc cagcgtggtt tattcaccgg gccgacgatt
     7921 aaattagaat ggtcctcatc gaagtattta tcgctacaca tcccataaac ttctgaagtt
     7981 gacgggaaga tgattcgctt acggtactta acgcagtagc ggataatgcg cagattctct
     8041 tcaaaatcga gttcaaatac gcgcagcggg ttgcgggtat attcaatcgg cgtggctatc
     8101 gccaccagcg gcaagacgac atcacatttt ttgacgtgat actcaatcca ttcggaatga
     8161 atactgatat cgccttcgac aaagtgaaaa tgcggatgat tcagaaaacg gcttatcgca
     8221 tcgctgccaa tatccagacc gtaaacttca taatgatctt cgcgcagcag gcgttctgtc
     8281 agatggttgc caataaagcc attcaccccg aggatgagta cccgggtacg gcgtcgggcg
     8341 gtgcaggcag gctggctatt caagcgtgaa ccttgcacca ggcccagcat ctgcgctaat
     8401 tgcgagccct gcatagtaat gccgtcgccc gcctgtccgg tgacgatttc cagcgcgcca
     8461 tcgccacagg caatcagcag tggcgcaaca gaaatcacgc tccccggctg tgctttgctg
     8521 gcatgaggat gaacacgcga cgaccagacg gtgaatttct gattgccaac atagctgaag
     8581 gcacccggcc acggatcggc aacggcacgt accatgttgt gcagtacgga tgccggttta
     8641 tgccattcaa ggaaactgtc atctggcgtt ctgtgaccaa aacaggtggc ttcgttttcg
     8701 cgctgggcaa tttccagaat attaccgtgt ttaatggcgg gtaatgtctg ttccagtagc
     8761 tggcgcgcgg catggcacaa tttatgatgc agcgtaatag cgatatcgtc tggcgcaatg
     8821 gcaacgcgca gttgcgccac aatggcccca gcatcggcac gttttaccat tcggtgcaat
     8881 gtaacgcccg tttccgtttc accgttcacc agcacccagt tcagcggcgc gcgaccacga
     8941 tattttggta acagcgaacc gtgcagatta aatgcacctg cgggagcgag ctgcaaaatc
     9001 tcgtcgcaaa taagatggcg ataataaaaa gagaaaatca cctctggtga gagttgggca
     9061 atgcgttcca cccacagcgg atgattaacg ttatccggcg cataaactgg aatgcctctt
     9121 tccgccgcca gacgagccac cgaaccataa aaggcttttt cagcgggatt atcggtatgg
     9181 gtaaaaatgg cgctaatttc gtaaccggca gccagcagag cttcaatacc gaggcatccc
     9241 atatcgtggt aggcaaaaac gacggttttc attattcatt ttccttgctg gatggacgga
     9301 taacttgctg aacaaaatag cgggggcggg cgcggacatc ggtgtagatc ctgccgatat
     9361 attcaccgag caatcccatg ccgataaact gagcgccaat aaaagtaaac agcacggcaa
     9421 atagcataaa gacgccttct gccgcccatt gtggtccgaa ggtcaggcgt aaaatcacca
     9481 gcagcaccgc aatgctaaaa cctccaatcg caataatgct gccgagcaga ctcagcatac
     9541 gtagcggcgt tgtggtaagg caggtcacca ggtcgtacat caaattaatc aggcgcataa
     9601 aactgtattt ggattcacca aactcacgct cggcatgatg tactggaatt tcaatggcac
     9661 ggcgggcgaa gatattcgcc agaatcggga taaaggtgct acgttcatgg cagtgcaaca
     9721 tcgcatcgac aatatgacgg cgataggcgc gcagcataca accgtagtca cccatcgctt
     9781 tgccggtggt gcgctgaata agcctgttaa tcatcttcga agctgtttta cgaaaccagc
     9841 tgtcctggcg gttctggcgt acagtcccta ccacgtcgta accttcatcg gcttttgcca
     9901 ccaggcgggg gatttcttct ggcggattct ggagatcggc atcaagggta ataattaagt
     9961 cgccagtaac gtgactgaat cccgccataa tcgctgaatg ttgcccgtaa ttgcggttaa
    10021 gcaaaataga cacaatatgg ctgttctccg cttgtgaggc ttcgaccagc atatgcgcgg
    10081 aattatcgct actgccgtca tcaatcagca ggatctcata ctctttcccc aacgattcac
    10141 aggctgtggt ggtgcgcctg attaattccg gtaagctttc ctgctcgtta taaacgggaa
    10201 taaccaccga gactttctta acagggtgga tttcaaacat ggcttattgt cctgcgagtt
    10261 gctgaagggc tgtgatgaca cggtcggcat cggcggtagt catatccggg aacagcggca
    10321 aagaacagat gcgttcgcta ttccattcgg tattcggtaa cgacagcgtg ggaaaacgct
    10381 cgcgataata tttttgtgtg tgagcggcgc ggaaatgtaa cccggtacca atgcctcttt
    10441 cttttaacgc ttccatcaac gcatcgcgac tgataccaca acgttgttca tcgacacgaa
    10501 taataaacag atgccaggcg tgaacgtgcg gccaggcggg aaggcttaat ggctgaaagg
    10561 ggagagctgc cagtgcttgc tgatattgct gggcaatttc gcgccgatgg gtgttgaggt
    10621 gctctaattt tgctaactgt gtcagggcaa tcgcggcgtt aatatcggtc agattgtact
    10681 tatagcccgg tgttaagact tcagcctgcg gtgcacggcc ccaggtttgt ctgtcatagg
    10741 catcgacacc cagaccgtga aatttcagca tccgtagctg gcgggcaagg ttttcattat
    10801 cagttacaat caggccacct tccgcacagg taatattttt aatggcatga aatgaaaaaa
    10861 tagcggtacc ttttgcgcca atatgtcgcc ctttgtaata cgtaccgacg gcatgggcag
    10921 catcttcgat aactgcgatg ccgtaacgtt cgccaatggc gcgaatggcg tcaatatctg
    10981 ctggcgcacc ggcataatgc accggaatga tggctttagt gcgtggcgta atggctgact
    11041 cgatcgcttc aggcgtgacc atcagcgtat cgcggtcgac atccaccatt accggcgttg
    11101 cacccaacaa ggaaatcata ttgagggttg aaacccaggt cagggaaggc gtaatcactt
    11161 catcgccctt gccaatttcc aacgccatta gcgtgatatg cattccggcg gtggctgaac
    11221 tgaccgcgat ggcatgctga tttcccgtca actggcaaaa ggcttgctca agcgcctgat
    11281 tcttcggacc ggttgtgatc caaccggatt cgagaacctc tttaactgca gcgagttcct
    11341 ccacgcccat tgctggtcgc gaaaaaggca aaaattctga cattgctttt ccttccgcca
    11401 ttgaatacct gtccacttat atttgctata aagagtgttg tgtatatttt gccatttgga
    11461 gcgaaatttt aaggatagaa tattaactta accttaagaa actaatatta gacgtaaata
    11521 ttgaaatttt tatatttttt cttatttagg ctttgcattt ggcaaaattt tgaggcattt
    11581 tgccgacatc gtaggatttt taatattaca ccaactgcta attatcgcca gaaatgtcgc
    11641 tcaatttcac ggtaattgtc tggttgcgct tgtctatagg tggagtttac gtgttagctt
    11701 tttgccgctc atcattgaag tcaaaaaaat atttcatcat tttactggcg ctcgctgcaa
    11761 ttgccggact gggtactcat gccgcctgga gtagcaatgg tttgccacgt atcgacaata
    11821 aaacattggc cagactggca cagcagcacc cggttgtcgt tttgtttcgc catgctgaac
    11881 gttgcgatcg ttcaaccaat caatgcttgt cagataaaac aggtattacg gttaaaggta
    11941 cccaggatgc ccgtgaactg ggcaacgctt ttagcgctga catccctgat ttcgatcttt
    12001 attccagtaa taccgtccgg accattcagt cggcgacctg gttttccgcg ggtaaaaaac
    12061 tgacggtaga taaacgactt cttcagtgcg gtaatgagat ttatagtgca attaaggact
    12121 tacaaagcaa agcgcctgat aaaaatatcg ttattttcac ccataatcat tgcctgacat
    12181 atattgctaa aaataagcgt gatgcgacat ttaaacctga ttatctggat ggtttagtca
    12241 tgcatgtgga aaaaggcaaa gtttatctgg atggggaatt cgttaaccac taaattttca
    12301 atctgacagc cagtgatggc tgtcatcatt gttaaagaag acctttcaaa cgtaacgttt
    12361 ttcgggtggc aacatttaaa tcataatgca ccagatcttc aggttttacc cacgcgtagt
    12421 cctgaaactc ttcgtttatt ttcacttctc ggttggcaga aacgcagtca aaaatcaggt
    12481 aaatcatata aatctcttcc ttgcgaccat ctgcatacgt cttggtgcga atatcatcgc
    12541 tgaaggtcca cggcgtgatt tctgtcaaaa gcagctgttc tcccagttct tcgcgaattt
    12601 cgcggcgtag tgcctcttca attcgttcgc cagactccac gccgccaccc gaaagcgccc
    12661 attgaccggg gaaaacgccg cgatcgtcgg ccattttgca cagcaaataa gcaccatcat
    12721 tttgaatcaa agggcataca atagtccgtt gtcgcacagg ggtattcctt ataaataaaa
    12781 agaaaccatg agaatacggt aactcagaaa tatgtgaaag tgacagtaat ctct
//
LOCUS       DAZOYL010000102        12821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZOYL010000102 DAZOYL010000000
VERSION     DAZOYL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.102"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..890)
                     /gene="yhaJ"
                     /locus_tag="ACHUOY_002092"
                     /pseudo
     CDS             complement(1..890)
                     /gene="yhaJ"
                     /locus_tag="ACHUOY_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            995..1696
                     /locus_tag="ACHUOY_002093"
     CDS             995..1696
                     /locus_tag="ACHUOY_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR0682870.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1719..1883
                     /gene="yhaL"
                     /locus_tag="ACHUOY_002094"
     CDS             1719..1883
                     /gene="yhaL"
                     /locus_tag="ACHUOY_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR0682871.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2017..3327)
                     /gene="cyuA"
                     /locus_tag="ACHUOY_002095"
     CDS             complement(2017..3327)
                     /gene="cyuA"
                     /locus_tag="ACHUOY_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR0682872.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3355..4686)
                     /locus_tag="ACHUOY_002096"
     CDS             complement(3355..4686)
                     /locus_tag="ACHUOY_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0682873.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4961..6325)
                     /gene="tdcG"
                     /locus_tag="ACHUOY_002097"
     CDS             complement(4961..6325)
                     /gene="tdcG"
                     /locus_tag="ACHUOY_002097"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0682874.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6397..6786)
                     /locus_tag="ACHUOY_002098"
     CDS             complement(6397..6786)
                     /locus_tag="ACHUOY_002098"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR0682875.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6800..9094)
                     /gene="tdcE"
                     /locus_tag="ACHUOY_002099"
     CDS             complement(6800..9094)
                     /gene="tdcE"
                     /locus_tag="ACHUOY_002099"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR0682876.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9128..10336)
                     /gene="tdcD"
                     /locus_tag="ACHUOY_002100"
     CDS             complement(9128..10336)
                     /gene="tdcD"
                     /locus_tag="ACHUOY_002100"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR0682877.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10362..11693)
                     /gene="tdcC"
                     /locus_tag="ACHUOY_002101"
     CDS             complement(10362..11693)
                     /gene="tdcC"
                     /locus_tag="ACHUOY_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR0682878.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11715..12704)
                     /gene="tdcB"
                     /locus_tag="ACHUOY_002102"
     CDS             complement(11715..12704)
                     /gene="tdcB"
                     /locus_tag="ACHUOY_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR0682879.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3280 a         3509 c         3058 g         2974 t
ORIGIN      
        1 ccgctaaaaa gtttgggaat ttcccgcaga caccaggatt tcgcttcccc catactgtca
       61 cgacgccagg ccataataat atcgatctca ctggtcgatt ccgggctgac gacacgcaac
      121 cgcccttccg caatatcttt ttcgaccatc ggatacggca tcgtcgccac gccaagcccc
      181 gccagtaatg cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg ctgtttgtcc
      241 agcagttgta cggtcaacac cgggcgctca cgagcggtat ccgctaccgc aattccacga
      301 tatttcacgc gcgtcacttc agataacggt tccggctcct gatgaatcgg gtgatcaggc
      361 gcggcaacgt agacgttcat taacgtatag agcttgcgcg agttgatctc cgacgaggaa
      421 cgaaaatgca tatccggcgc gataacaata tccgcccgcc cctgctccag tcgttcctac
      481 gcccccgcca gcacttcggt gatgattgcc agttgggtat tggcttttgc cgccagtttg
      541 tcgattaacg ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat ggtgagatgc
      601 gtttcccaac cgcgcgcgag ggcttccgca tcggtagtca gtttatctgc ggcttccagc
      661 aaaacgcgcc ctcgctccag caacatcctc ccgacattgg tgaatttggt gcgatggccc
      721 gagcggtcaa acagcaccac atccagctct tcttccagtt tttgcatggt gtagctaagt
      781 gcggaaggca cgcgtcccag ctcatccgcc gccgccgcaa aactgccccg gcgatcgatc
      841 gcatccataa cccgtagtgc ttccagcgtt aatgcccttt ctttggccat ttcgttctca
      901 ttcaggaaat ttgaacatac cgggcagaat atctggctaa caatgtagcg tccagcccct
      961 taccataaaa ggaagtaaag agaggtcaag aattatgatt actacccgaa ctgccaggca
     1021 gtgtggacaa gcagactacg gatggttgca ggcccggtat actttttcct ttggacacta
     1081 cttcgacccg aaattgttag gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc
     1141 cccaggtgcc gcctttcagc cgcgaaccta tcccaaagtc gatattttaa atgtgattct
     1201 ggatggagaa gcagagtatc gcgacagcga aggcaatcat gttcaggcca gcgccggtga
     1261 ggcgttgctg ctctctaccc agccgggtgt cagctacagc gaacacaatc tcagcaaaga
     1321 caaaccgtta acacgaatgc agctttggct ggacgcctgc ccgcagcgag agaatccgct
     1381 gattcaaaaa ctggcgctta atatgggcaa gcagcaatta atcgcctcgc cagagggtac
     1441 gatgggaagc ctgcaattac gccagcaagt gtggctgcac catatcgtgc tcgacaaagg
     1501 cgaaagtgcg aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa
     1561 atttcatgcg cttacgcatc atgaagagaa agcggcgctg acctgcggtg atggggcgtt
     1621 tattcgtgac gaggctaaca ttacgctggt tgccgattcc ccactgcgcg ctttgctgat
     1681 agatttgcct gtctagttgt ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa
     1741 agcgccagcc agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg
     1801 gctatgaaga gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag
     1861 ccgaggatga agctaccgcg taaagacagg ttgccggatg cggcgaaaac gccttatccg
     1921 gcctacaaaa tccaacaaca ttaacgaatt aaacaacccg taggccggat aaggtgcgcc
     1981 agcatcgcat ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat
     2041 ctcgataatc tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc
     2101 aatcgactgc tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc
     2161 cagcgccatt aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca
     2221 gctgttcgac gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc
     2281 catcgagata gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat
     2341 tgctgcggtc gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat
     2401 tgcgctcaaa tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg
     2461 ttctgctaca accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt
     2521 actcatagcc ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat
     2581 cacaatggat gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt
     2641 cgcgccaata tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag
     2701 cttcgcggaa tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag
     2761 gatctcagcc agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc
     2821 cacacacgcc tgctgggtaa acaccacacc attgtgcgtc tcaatacgca caatgttggt
     2881 atgcccgccg acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga
     2941 gaagaggatt tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag
     3001 tgctttggca tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt
     3061 ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc
     3121 gggaacggtg acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg
     3181 ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg
     3241 ttcagtacat cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca
     3301 taacggattt aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga
     3361 aaatgccagg aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga
     3421 catccctttg tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca
     3481 ccctaccatg ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag
     3541 tacgatagcg ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt
     3601 ttcgttaatc ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt
     3661 tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag
     3721 aatgacgctg accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat
     3781 cgccagcgcg gaaatattct gctcgtaggc ttttaccgct tcgtcatgcc ccatcgccag
     3841 cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat
     3901 tgcccgcaat gctttatgcc gcgccacttc aatagatttt tcccgcgagc gataagagat
     3961 caccatcggg cttaacgtct ggataaacaa aatcgacgtc agggtaaacg gcagcgtaat
     4021 aatggcgttt ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca
     4081 cattcccacc atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc
     4141 ggtcgaaatt ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat
     4201 cagtaccaga ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg
     4261 cagataggaa gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag
     4321 cattacgaaa tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat
     4381 gacactcggg taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg
     4441 aaacagatac attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat
     4501 caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat
     4561 aatcacccag ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact
     4621 cattccagca cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcgat
     4681 ttccataatt tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa
     4741 ggcgatatgc ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg
     4801 aggtcagaga aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc
     4861 tactaaatta atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg
     4921 tgcacatttg tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa
     4981 tggccagtcc tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat
     5041 acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca
     5101 tccacgcggc gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt
     5161 gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg
     5221 ccgcattgca tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca
     5281 ttgaacaggc cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt
     5341 tcattttata cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct
     5401 cgtttaccgg acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg
     5461 caccgttagt cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca
     5521 gcgcgtacat gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag
     5581 aaaccagctg acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca
     5641 cgccttcagt gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa
     5701 atcctgcgtc aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag
     5761 atatagacag gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt
     5821 aaggtacgga cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc
     5881 cgccgccgac agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga
     5941 tccgcattcc gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta
     6001 caggaaaatc aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta
     6061 actctataaa tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc
     6121 ccatgataat ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca
     6181 gatcgaccac aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa
     6241 aactttttcc ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt
     6301 tgaaaatatc gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga
     6361 gcggaacgac cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc
     6421 ttcaatttcc agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg
     6481 gtaggtcgcc tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc
     6541 attcagatcg gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc
     6601 aaccacgatc gctttgacgt tttcgaggct taaacacgcc tgatcttgca catcagccgg
     6661 gatctcaccg gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa
     6721 atcaacgccc tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt
     6781 tttcatacat cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt
     6841 gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt
     6901 taggatattt ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga
     6961 cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc
     7021 cgacaaggtt ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga
     7081 aggtgtacga gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg
     7141 aggccacggc acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg
     7201 ttccggcgcg acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag
     7261 tgatagtcag aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt
     7321 tcttcataaa gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc
     7381 cgtactgcgg atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga
     7441 ttggtttcac gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg
     7501 cgatgccgca tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt
     7561 cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg
     7621 ccagccagtc catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt
     7681 ccatcagcgg tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt
     7741 taattgcgta cagcagtgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct
     7801 taccaatcac cattgggctg acgcaacaag caattgcgta atcgtcgctg ttgaagtcag
     7861 tacgcatcag atcgtcgttt tcatactgca aggaagaggt gacgatcgac acctgcgcgg
     7921 catatttttt gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg
     7981 gtgccggccc catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg
     8041 tacgaccgtc cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga
     8101 acagcgaatc aaattccggc gtacgcaaga agcgcaccat acggatcttc ataatgaagt
     8161 gatcgatcag ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa
     8221 tgtagatatc gaggaacgac gccgtgcgcc ccagcgacat cgcgccgccg ttctgtgatt
     8281 tcactgccgc cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg
     8341 ggcgagagat atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac
     8401 gatgctctgc cagctcctca cgcagacgga tggtggcttc cagatcttcg cctttttcca
     8461 gacgggactg gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat
     8521 acagcgctac gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac
     8581 cggtcagcac gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc
     8641 cctggttatg ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac
     8701 ggccataggc gtggaatgaa cttttaatca tattaatgcc accgaacgga tgcagcgcgc
     8761 gctttaatgg cgcatccgtt tgcagaccaa caattttttc cagcggctgg ttaatatatc
     8821 ccgcatcatg agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg
     8881 ttgcgttttc gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg
     8941 tcgcttcggc gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat
     9001 cgcggacatt aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt
     9061 acagcttatc gctggtatca atatctatct tcatgaaaaa taatctctct acaatacttc
     9121 aactaaatta tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag
     9181 caatcatttt ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg
     9241 aaacaattcg ctctccaaaa gagttagagc gattattcat ttctgtatca atcactacgc
     9301 ctaatacagc caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc
     9361 cgccggtgaa tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac
     9421 gggcaattcg gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc
     9481 aggctttttc cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact
     9541 ctttattcac tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg
     9601 acatcgcacc aaagtcgaca tcgccactgc gggttcccat catcaagcct tccagcggcg
     9661 tcattcccat tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat
     9721 tgccaagatg cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgtg
     9781 cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact
     9841 cttcataata tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga
     9901 aactggtatc aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa
     9961 taccacttaa attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat
    10021 caatgacttc atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc
    10081 ggtggccaat taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg
    10141 ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg
    10201 ataagaatgc attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt
    10261 cgctggcatc gagcacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa
    10321 ccggaaattc attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg
    10381 atgttcagga tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg
    10441 tatttcgcca gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat
    10501 gcgataatcg gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca
    10561 acaacccagg tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac
    10621 actttagttt tgtcgccttt ataaccaaac ttcaggacca ggccattcag accttccagc
    10681 gttcccagat agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc
    10741 gcatattcca gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta
    10801 gccagataag aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac
    10861 agcgtaaata ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca
    10921 cgagaaatga tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac
    10981 tcttcacgtt tagaaaccac gaaggaagag acgattggcg agaaattaaa ggagaaaacc
    11041 atgatggaaa tccccagcca gacggtgatc aggataccgt catgaccggt taacgacagc
    11101 gaaccgaggt caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa
    11161 atcagcacca ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga
    11221 tccttaccaa accagatgac gaaagccatc agcagcagca ggaacagcgc cacaaagccg
    11281 cgattcagcg gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata
    11341 gtaacgccat aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc
    11401 acgccgccag ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta
    11461 gagccagaaa gacatagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc
    11521 aataccaaca tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac
    11581 agcaccccgg cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta
    11641 cgccaggacg attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat
    11701 cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg
    11761 atattgccgc cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta
    11821 ccgcttaata atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc
    11881 tgaattaagg caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg
    11941 actaattcac gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc
    12001 gccagggtgc cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg
    12061 ccgtgaacgt tttcagactg tacgccaata acacgaatgg tcgggttaat agatttaatt
    12121 gccaccgcaa taccagcaat taaaccgcca ccaccaattg gcacaatcac gttatcgaca
    12181 tcatagagat cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga
    12241 tcatcgtaag gtgggataaa aatacggcct tccatttcga caatttcgct cactttagcg
    12301 atagtgtcgt tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc
    12361 gctactttgg attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc
    12421 gcgcaggaga gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg
    12481 cgtttttccg catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa
    12541 cccgtacgct gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa
    12601 tagttggagc gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca
    12661 ataatatcat caatagcaat cggcagatcg tatgtaatat gcattataaa acctcttcgc
    12721 ctgaattaaa tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt
    12781 atgtttaaat aaaaacaaat aactaaccaa cttctattaa t
//
LOCUS       DAZOYL010000103        12802 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZOYL010000103 DAZOYL010000000
VERSION     DAZOYL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.103"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(178..1065)
                     /gene="rarD"
                     /locus_tag="ACHUOY_002103"
     CDS             complement(178..1065)
                     /gene="rarD"
                     /locus_tag="ACHUOY_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR0682880.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1117..1584)
                     /gene="yigI"
                     /locus_tag="ACHUOY_002104"
     CDS             complement(1117..1584)
                     /gene="yigI"
                     /locus_tag="ACHUOY_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR0682881.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1749..2618
                     /gene="pldA"
                     /locus_tag="ACHUOY_002105"
     CDS             1749..2618
                     /gene="pldA"
                     /locus_tag="ACHUOY_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR0682882.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2751..4580
                     /gene="recQ"
                     /locus_tag="ACHUOY_002106"
     CDS             2751..4580
                     /gene="recQ"
                     /locus_tag="ACHUOY_002106"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR0682883.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4644..5261
                     /gene="rhtC"
                     /locus_tag="ACHUOY_002107"
     CDS             4644..5261
                     /gene="rhtC"
                     /locus_tag="ACHUOY_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR0682884.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5323..5943)
                     /gene="rhtB"
                     /locus_tag="ACHUOY_002108"
     CDS             complement(5323..5943)
                     /gene="rhtB"
                     /locus_tag="ACHUOY_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR0682885.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6054..7076
                     /gene="pldB"
                     /locus_tag="ACHUOY_002109"
     CDS             6054..7076
                     /gene="pldB"
                     /locus_tag="ACHUOY_002109"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR0682886.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7084..7884
                     /gene="yigL"
                     /locus_tag="ACHUOY_002110"
     CDS             7084..7884
                     /gene="yigL"
                     /locus_tag="ACHUOY_002110"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR0682887.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7960..8859
                     /gene="bioP"
                     /locus_tag="ACHUOY_002111"
     CDS             7960..8859
                     /gene="bioP"
                     /locus_tag="ACHUOY_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR0682888.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8747..9700)
                     /gene="metR"
                     /locus_tag="ACHUOY_002112"
     CDS             complement(8747..9700)
                     /gene="metR"
                     /locus_tag="ACHUOY_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR0682889.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9936..12197
                     /gene="metE"
                     /locus_tag="ACHUOY_002113"
     CDS             9936..12197
                     /gene="metE"
                     /locus_tag="ACHUOY_002113"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR0682890.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12482..12727)
                     /locus_tag="ACHUOY_002114"
     CDS             complement(12482..12727)
                     /locus_tag="ACHUOY_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR0682891.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3009 a         3225 c         3431 g         3137 t
ORIGIN      
        1 ttgggttttg tcaaatcctt ggtagatata cgatgtctct aggatggagt aagtactggg
       61 gagttttggg ggtatttcct gtaacaaata ttgttctttt tcttatctta atttttttca
      121 aaaaaagata aataagtcag tgggtaggct ctttaaacgg cctacccatg ttaattctta
      181 actttttcta cgctgcgtat aaatcgcatc catcacaaaa attgccagcg ccacccaaat
      241 aaaggcgaaa gtcaccatct tatcggcacc cggtttttca ccataaaacg tcacagccag
      301 caggaacatc agcgtcgggc caatgtactg gaaaaagcct aacgttgaga gacgcaagcg
      361 cgtggcagcg gcggtaaaac acaacagcgg tacggtagtg acaataccgg cggcgatcag
      421 cagtaaattc agcgacatcg ggttttgccc catatggctg gttgagctgt cggcaatagc
      481 aaacaggtaa attgccgcca cgggcagcag ccacatggtt tcgattaaca tgccggtttg
      541 cgcttcaacg gcaatcttct tgcgtaccag accgtagaag gcaaaactaa atgccagtcc
      601 cagcgcgata ataggtagcg aaccaaaagt ccacagctgg actaacacgc cacatatcgc
      661 cagaatcacc gccagccatt gcatccggcg gaatcgctcg ccgaggaaaa tcatccccag
      721 cacaatgttc accagcgggt taataaagta accaaggctc gcttccagca tatggtgatt
      781 gttcaccgcc cagataaaca gtagccagtt gccaccaatc agcacggcag agactgccag
      841 cataaaaatt ttctgtggcg tctgaatcag cgtttttaaa taggaccact ggcggcaaat
      901 gctcatcagc accaccataa agaaaaacga ccagatcacg cgatgcgtca ggatttcatc
      961 ggcgggcacg tagtaaatca acttgaagta cgctggcgct ataccccaaa taaaataagc
     1021 ggcaagagcg agtaatacgc cctgccgcgt ttgttttgca tccatcggga atactcattt
     1081 ttaattggta acagcagttt acctgctttt atgtcttcaa cctaccatat aggtggcggt
     1141 ggcactggca atataaagct gttcttcatt gtgtaattca acgcgggcga cggcgacttt
     1201 attgcctgca cgcaacaggc tactggtagc agtaaaacgc tcgcccctgc ctgggcgcag
     1261 ataatcaacg cgaagatcaa tggtccccat ccgcgatagc cgctggcgta gttcatcttc
     1321 actgatggtt tcgtggcggg ttaaggtact tcccacgcac accagaccgg cggcgacatc
     1381 cagcgccgac gcaatgaccc cgccgtgcaa aatgctttgc gcccagttgc ccaccatcat
     1441 tggctgattt ttaaaggcca gctgtgcgaa ctctttttcg taacgctcca gttccatccc
     1501 caatgcgcgg ttaaatggca tgtgataaac aaacatctca cccactaatt tcagggcttg
     1561 ttcagcggtc agtacggcag acatatcatc cttacacttc attggttaat gaaatgttga
     1621 ttttatgctt ctttgttgtt ggtttctact ttaggaaggg ataactaacg gctatggagt
     1681 taagtatgta aaatagcccg cagaaaaata ttcaccttat caataattcg ttacggagaa
     1741 cacgaccgat gcggactctg cagggctggt tgttgccggt gtttatgttg cctatggcag
     1801 tatatgcaca agaggcaacg gtgaaagagg tgcatgacgc gccagcggtg cgtggcagta
     1861 ttatcgccaa tatgctgcag gagcatgaca atccgttcac gctctatcct tatgacacca
     1921 actacctcat ttacacccaa accagcgatc tgaataaaga agcgattgcc agttacgact
     1981 gggcggaaaa tgcgcgtaag gatgaagtaa agtttcagtt gagcctggca tttccgctgt
     2041 ggcgtgggat tttaggcccg aactcggtgt tgggtgcgtc ttatacgcaa aaatcctggt
     2101 ggcaactgtc caatagcgaa gagtcttcac cgtttcgtga aaccaactac gaaccgcaat
     2161 tgttcctcgg ttttgccacc gattactgtt ttgcaggttg gacgctgcgc gatgtggaga
     2221 tggggtataa ccacgactct aacgggcgtt ccgacccgac ctcccgcagc tggaaccgcc
     2281 tttatactcg cctgatggca gaaaacggta actggctggt agaagtgaag ccgtggtatg
     2341 tggtgggtaa tactgacgat aacccggata tcaccaaata tatgggttac taccagctta
     2401 aaatcggcta tcacctcggt gatgcggtgt tgagtgcgaa aggacagtac aactggaata
     2461 ccggttacgg cggcgcggag ttaggcttaa gttacccgat caccaaacat gtgcgccttt
     2521 atactcaggt ttacagcggc tatggcgaat cgctcatcga ctataacttc aaccagaccc
     2581 gtgtcggtgt aggagttatg ctaaacgact tgttttgatg aacggttgag tggttggcaa
     2641 atctggaacc cagcatccag gattaccctc tcagagacta aaagcattgc agtttctcgc
     2701 gcaggcgctg aaaatagcgc ctgtttttat ttcaggcaat cggggggaat gtggcgcagg
     2761 cggaagtgtt gaatctggag tccggagcta aacaggtttt acaagaaacc tttggctacc
     2821 aacagtttcg ccccggccag gaagaaatta tcgacactgt gctttccggt cgcgattgcc
     2881 tggtcgtcat gcccaccggt ggcggaaaat ccctttgcta tcaaattcct gccttattgc
     2941 taaacggcct taccgtggtt gtttcaccgc tgatttcgtt gatgaaagat caggtggatc
     3001 aactgcaagc caacggcgtg gcggcggcgt gccttaactc gacgcaaacc cgcgagcagc
     3061 aacttgaagt gatgacaggc tgccgcaccg ggcaaattcg tctgctttat atcgccccgg
     3121 aacgcctgat gctggataac tttcttgagc atctggcgca ctggaatccg gtgttattag
     3181 ccgttgatga agcgcactgt atctcccaat ggggccacga tttccgcccg gaatatgccg
     3241 cgcttggtca gttacgccag cgtttcccga ccctgccgtt tatggcgctg actgccacag
     3301 ccgacgacac cacgcgccag gatatcgtgc gcttgctggg gctgaacgat ccgctgattc
     3361 aaatcagcag ttttgaccgt ccgaatattc gctacatgct gatggagaaa ttcaaaccgc
     3421 tcgatcagtt gatgcgctac gtgcaggaac agcgcggtaa gtccggcatt atctactgca
     3481 acagccgggc gaaagtagaa gacaccgctg cgcgcctgca aagcaagggt attagcgcgg
     3541 cggcctatca tgccgggctg gaaaataatg tccgcgccga cgtgcaggag aaattccagc
     3601 gcgatgacct gcaaattgtg gtggcgacgg tggcgttcgg catgggcatc aataaaccaa
     3661 acgttcgctt cgtggtccac tttgatattc cgcgcaatat cgaatcctat tatcaggaaa
     3721 ccggtcgcgc cgggcgtgat ggcctgcctg cggaagcgat gctgttttac gatccggctg
     3781 atatggcgtg gctgcgccgt tgtctggaag agaagccgca ggggcagttg caggatattg
     3841 agcgccacaa actcaatgcg atgggcgcgt ttgccgaagc gcaaacttgc cgtcgtctgg
     3901 tattgctgaa ctattttggc gaagggcgtc aggagccgtg cgggaactgc gatatctgcc
     3961 tcgatccgcc gaaacagtac gacggttcaa ccgatgctca gattgccctt tccaccattg
     4021 gtcgtgtgaa tcagcggttt gggatgggtt atgtggtgga agtgattcgt ggtgctaata
     4081 accagcgtat ccgcgactat ggtcatgaca aactgaaagt ctatggcatg ggccgtgata
     4141 aaagccatga acattgggtg agcgtgatcc gccagctgat tcacctcggc ctggtgacgc
     4201 aaaatattgc ccagcattct gccctacaac tgacagaggc cgcgcgcccg gtgctgcgcg
     4261 gcgaatcctc tttgcaactt gccgtgccgc gtatcgtggc gctcaaaccg aaagcgatgc
     4321 agaaatcgtt cggcggcaac tatgatcgta aactgttcgc caaattacgc aaactgcgta
     4381 aatcgattgc cgatgaaagt aatgtcccgc cgtacgtggt gtttaacgac gcaaccttga
     4441 ttgagatggc tgaacagatg ccgatcaccg ccagcgaaat gctcagcgtt aacggcgttg
     4501 ggatgcgcaa gctggaacgc tttggtaaac cgtttatggc gctgattcgt gcgcatgtcg
     4561 atggcgacga cgaagagtag tcagcagcat aaaaaagtgc caggatgaag actccgtaaa
     4621 cgtttccccc gcgagtcaaa tgtatgttga tgttatttct caccgtcgcc atggtgcaca
     4681 ttgtggcgct tatgagcccc ggtcccgatt tcttttttgt ctctcagacc gctgtcagtc
     4741 gttcccgtaa agaagcgatg atgggcgtgc tgggcattac ctgcggtgta atggtttggg
     4801 ctgggattgc gttgcttggc ctgcatttga ttatcgaaaa aatggcctgg ctgcatacgc
     4861 ttatggttgg cggtggcctg tatctctgct ggatgggtta ccagatgctg cgcggcgcgc
     4921 tgaaaaaaga ggcggtttct gcacctgcgc cacaggtcga gctggcgaaa agtgggcgca
     4981 gtttcctgaa aggtttactg accaatctcg ctaatccgaa agcgattatc tactttggct
     5041 cggtgttctc attgtttgtc ggtgataacg ttggcactac cgcgcgctgg ggcatttttg
     5101 cgctgatcat tgtcgaaacg ctggcgtggt ttatcgtcgt tgccagcctg tttgccctgc
     5161 cgcaaatgcg ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt
     5221 tatttgccgg atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga
     5281 gtaagtcgga taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga
     5341 tgctaacagc gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat
     5401 ctgctttggt cctttaatcc atagagcaat ccgttgagca agggtggcgt aaccgatcat
     5461 cacaataata tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg
     5521 ctgttgcggc atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt
     5581 ggtgagattc acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag
     5641 cgatttaagg tcaattgcac cagcggcgcg ccactgctgg attcccagcc aaatcaagta
     5701 agccgcgcct gcccacttca acacttcaaa cgcaatcact gagcgggaaa atagcgtccc
     5761 caaccccacg ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga
     5821 cgccaccgcg ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga
     5881 gcctggcgac aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt
     5941 catgatgaac tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt
     6001 gtcaggcacg ctaaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc
     6061 agcagcaaaa agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc
     6121 tgactgattt ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg
     6181 tgcgctttgt ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc
     6241 gtattgagag ctacgtaaaa tatgcggaac tggcctatga cctgttccat ttggggtttg
     6301 atgtcttaat catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc
     6361 atctcgggca tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc
     6421 aggaggttca gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg
     6481 cgatctccac attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg
     6541 cgccaatgtt tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact
     6601 gggccgaagc gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg
     6661 cgttgccgtt tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac
     6721 gcttctatgc tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg
     6781 aaagtattct ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgccaacgc
     6841 ttctcttgca ggctgaagag gaacgcgtgg tggataaccg catgcatgac cgtttttgtg
     6901 aactccgcac cgccgcgggc catcctgtcg aaggaggacg accgttggta attaaaggtg
     6961 cttaccatga gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccattg
     7021 ttgatttttt caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt
     7081 cttatgtacc aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg
     7141 ttatcccctt acgccaaaga aaccctgaag ctgctcaccg cgcgcggcat caactttgtg
     7201 tttgcgaccg gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagatcaag
     7261 tcgtacatga tcacctccaa cggcgcgcgc gttcacgatc tggatggcaa tctgattttt
     7321 gctcataacc tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatctg
     7381 gacatcatta ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa
     7441 gagatgcgct tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc tggattactg
     7501 gagccggaag gcgtcagcaa agtgttcttc acctgcgatt cccatgaaca actgctgccg
     7561 ctggagcagg cgattaacgc tcgttggggc gatcgcgtca acgttagttt ctctacctta
     7621 acctgtctgg aagtgatggc gggcggcgtt tcaaaaggcc atgcgctgga agcggtggcg
     7681 aagaaactgg gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgatgcc
     7741 gaaatgctgt cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa
     7801 gaccttcatc ccgagctgga agtgattggt actaatgccg acgacgcggt gccgcattat
     7861 ctgcgtaaac tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt
     7921 cgctacaatg gatactcgtt aatcaaagag ttttccattg tggcgctact tatcatcacc
     7981 acgattctgt gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat
     8041 agctattttg cggtgctggt gcgcgttggc ctggcggcac tcgtttttct gccgtttctg
     8101 cgtacccgtg gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag
     8161 ctcggcgtga tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg
     8221 ttgctgttca ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag
     8281 cgccgtctgc gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt
     8341 attcgctatg atcaggttac cgaccatttc tggactggct tgttgctggt gcaactctcc
     8401 aatatcactt ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg
     8461 ccgcagcata acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca
     8521 tggttcttgc tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg
     8581 gtgtttcttg gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg
     8641 caggtggacg ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg
     8701 gtaaacctgg ctatctggca ccaacagccg cactggccaa cgttcattac aggcgcgctg
     8761 gtgatcctgg cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg
     8821 gcagatgatc gcaggcgtga ttgcgcgctg agcgaataaa tgcttctgta atcggctgac
     8881 gctgctcgcc atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt
     8941 tggtcaccac cagaccttgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa
     9001 tacccatccg cgcggcaacc atctgaatca acaataaggt gttatcgacg cttttcagtg
     9061 acgggctgac gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca
     9121 ccggataaat caacagcgtc tcgctggcga gatcttccgg tgtaattcgc gttttcgccg
     9181 ccagtggatg gtcaggtgct aacaccagac gcacttcata gtcgaacatc ggcgaataat
     9241 gcaggccact gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca
     9301 acgcaggctg cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt
     9361 tcttatggaa attttctaac gcgggcgtca gccactgaat acagctatgg cactcaatgg
     9421 caatgcgcag acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg
     9481 gcagcacctg gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct
     9541 ggctcttacg cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag
     9601 acagggcgga ttgcgtctga tgcaacgtcg ccgcagcggc tgcgagagag ccgcagttcc
     9661 gcaacgcttg tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca
     9721 tgaataattt gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg
     9781 gacggctaaa atccttcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg
     9841 gtcacttact tcagtaagct cccggggatg aataaacttg ccgccttccc taaattcaaa
     9901 atccatagga tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg
     9961 gtttccctcg cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg
    10021 ggaactccac gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg
    10081 atcaacaaaa gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc
    10141 atgtactgac caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg
    10201 gttcggtaga tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac
    10261 ctgcggcggc agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg
    10321 agttcgttaa aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg
    10381 aggcgctggc gctgggccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt
    10441 ggctggggaa agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc
    10501 cggtttatca gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg
    10561 atgaacccgc gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt
    10621 acgacgcgct ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc
    10681 caaatctcga cacgattact gcgctgcctg ttcagggtct gcatgttgat ctcgtacatg
    10741 gtaaagatga cgttgctgaa ctgcacaagc gcctgccttc tgactggttg ctgtctgcgg
    10801 gtctgatcaa tggtcgtaac gtctggcgcg ccgatcttac cgagaaatat gcgcaaatta
    10861 aggacattgt cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc
    10921 ccatcgacct gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg
    10981 ccctacaaaa atgccatgaa ctagcactgc tgcgcgatgc gctgaacagt ggtgacacgg
    11041 cagctctggc agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata
    11101 atccggcggt agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg
    11161 tctatgaagt gcgtgctgaa gcccagcgtg cacgttttaa actgccagcg tggccgacca
    11221 ccacgattgg ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa
    11281 agggcaatct cgacgccaat aactaccgca cgggcattgc ggaacatatc aggcaggcca
    11341 ttgttgaaca ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg
    11401 acatggtgga atactttggc gagcacctcg acgggtttgt ctttacgcaa aacggttggg
    11461 tacagagcta cggttcccgc tgcgtgaagc caccgattgt tattggtgac gttagtcgcc
    11521 cggcaccgat taccgtggag tgggcgaagt atgcgcaatc tctgactgat aaaccggtga
    11581 aagggatgct gactggcccg gtgactattc tctgctggtc gttcccgcgt gaagatgtca
    11641 gccgtgaaac catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg
    11701 aagccgctgg aattggcatc atccagattg acgaaccggc tctgcgcgaa ggtttaccgc
    11761 tgcgtcgtag cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg
    11821 ccgccgtggc gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg
    11881 acatcatgga ttcgattgcg gcactggacg cagacgtcat caccatcgaa acctcgcgtt
    11941 ctgacatgga gttgctggag tcgttcgaag agtttgatta tccgaatgaa atcggtccag
    12001 gcgtctatga cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga
    12061 agaaagcggc aaaacgcatt ccggcagagc gcctgtgggt taacccggac tgtggcctga
    12121 aaacgcgcgg ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga
    12181 atttgcgtcg gggatgatga atattaacga gtcactgtaa attgcagtac gatgaaagag
    12241 gcaaaactgc ctgatactag gcctacataa tttatgcaat ttattgaatt tttgggtttt
    12301 gttggccgga caaggcgttc acgccgcatc cggcatgaac aacgcgcact ttgccaataa
    12361 tctgatggct tccatcaccg gaagccattt tgctatctgc gcccgttcag atacagaaat
    12421 aaccgaatca tttttacgca aaatataatc ctgagcgatt tgttcagtaa tcaacgctac
    12481 gttatttatt ctcacttttc acgaaattgt atcccttttc agtaacgtgg tagtgaggca
    12541 tagacttatg tccgctatcc tcaataataa ccaaaccctc gttagctaat ttgttaacaa
    12601 tgttaacaac attaccgaat cctggaattt gccttatgga tagtgtgcga tcgagattca
    12661 tccagttcca gtttatatct ttctcagcca taattttcag tacgttaatt tcagtttcag
    12721 ttatcatagt caagtgcatc ctggtggtat aagtcattaa gttctccctt aagtttataa
    12781 tcttctagag tagcagtatg cg
//
LOCUS       DAZOYL010000104        12710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZOYL010000104 DAZOYL010000000
VERSION     DAZOYL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.104"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            12..1331
                     /gene="idnT"
                     /locus_tag="ACHUOY_002115"
     CDS             12..1331
                     /gene="idnT"
                     /locus_tag="ACHUOY_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR0682892.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            1398..2396
                     /gene="idnR"
                     /locus_tag="ACHUOY_002116"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             1398..2396
                     /gene="idnR"
                     /locus_tag="ACHUOY_002116"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR0682893.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            2474..3976
                     /locus_tag="ACHUOY_002117"
     CDS             2474..3976
                     /locus_tag="ACHUOY_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR0682894.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4095..5177)
                     /gene="lptG"
                     /locus_tag="ACHUOY_002118"
     CDS             complement(4095..5177)
                     /gene="lptG"
                     /locus_tag="ACHUOY_002118"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0682895.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5177..6277)
                     /gene="lptF"
                     /locus_tag="ACHUOY_002119"
     CDS             complement(5177..6277)
                     /gene="lptF"
                     /locus_tag="ACHUOY_002119"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0682896.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6340..6390)
                     /gene="ytgA"
                     /locus_tag="ACHUOY_002120"
     CDS             complement(6340..6390)
                     /gene="ytgA"
                     /locus_tag="ACHUOY_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR0682897.1"
                     /translation="MSIIILATISTTQENK"
     gene            6544..8055
                     /gene="pepA"
                     /locus_tag="ACHUOY_002121"
     CDS             6544..8055
                     /gene="pepA"
                     /locus_tag="ACHUOY_002121"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR0682898.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8189..8632
                     /gene="holC"
                     /locus_tag="ACHUOY_002122"
     CDS             8189..8632
                     /gene="holC"
                     /locus_tag="ACHUOY_002122"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR0682899.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8632..11487
                     /gene="valS"
                     /locus_tag="ACHUOY_002123"
     CDS             8632..11487
                     /gene="valS"
                     /locus_tag="ACHUOY_002123"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR0682900.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11543..>12710)
                     /locus_tag="ACHUOY_002124"
     CDS             complement(11543..>12710)
                     /locus_tag="ACHUOY_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR0682901.1"
                     /translation="PSHSFVFHGTGERYFLICVVNVLLTIITLGIYLPWALMKCKRYL
                     YANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCVLTLSLLVLLI
                     FMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTVFFISTKMLHA
                     NSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDATYCIK
                     YAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQRKMIIAQLIY
                     YFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGL
                     AYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         3250 a         3220 c         3305 g         2935 t
ORIGIN      
        1 agataaagac tatgccatta atcattattg cggcaggcgt cgcgctgctt cttatcctga
       61 tgatcggctt taaagttaac ggctttattg ccctcgttct ggtagctgcc gtcgtcggat
      121 ttgccgaagg gatggatgca caggccgtcc tgcactctat acaaaatggt atcggcagca
      181 cgctcggcgg gctggcaatg atcctcggtt tcggggccat gttaggcaag ctgatttctg
      241 atacgggcgc ggcacaacgt atcgccacta cgctgattgc tacttttggt aaaaaacgcg
      301 tgcaatgggc gctagtgatc accggtctgg ttgtgggcct cgccatgttt tttgaagtgg
      361 gttttgtcct gctgttgccg ttggtattta ccatcgtggc atcatctgga ttacccctgt
      421 tgtatgttgg cgtaccgatg gtagcagcgc tctctgtaac ccactgtttt ctgccgccac
      481 atccagggcc tactgccatc gcgactatct ttgaggctaa tctcggaacg actttactgt
      541 atggatttat cattaccatt ccgacagtta ttgtcgcagg accgctgttt tctaaactgc
      601 taactcgctt tgagaaagca ccaccggaag gcttatttaa tcctcatctg tttagcgaag
      661 aggagatgcc ctccttctgg aacagtattt ttgctgcagt gatcccggtc atcctgatgg
      721 ctatcgccgc cgtttgtgaa attacgttac cgaaaactaa caccgtgcgc ctcttctttg
      781 aatttgtcgg taaccctgcc gttgcgctgt ttattgccat tgttattgcg attttcacac
      841 tgggccgacg taatggacgc accatcgagc aaatcatgga tatcattggg gattctatag
      901 gcgctatcgc gatgattgtg tttattatcg ctggcggcgg cgcgtttaag caggtattag
      961 tagatagtgg tgtcgggcag tatatttcac acttaatgac cggaactacg ctttcgccgt
     1021 tattgatgtg ctggactgtc gcggcgctgt tgcgtatcgc tctgggctct gccaccgtcg
     1081 cggccattac caccgcgggt gtggtgttgc cgattatcaa cgttacccat gccgatcccg
     1141 ctttaatggt actggcaact ggtgcgggca gcgtgatcgc gtcacacgta aacgaccctg
     1201 gcttctggct atttaaaggg tattttaatc tgacggttgg tgaaacgttg cgtacctgga
     1261 cagtgatgga aacccttatt tctattatgg gtttgctggg cgtgttagcc attaacgccg
     1321 tattgcactg aaagaataaa atattgtgat gtagtttcgg gggaagattg aagattcccc
     1381 cgacgttggg agaagttatg cgcaatcaca gaatttcttt acaggatatc gctacgctgg
     1441 ctggcgtaac aaaaatgacc gtgagtcgtt atatccgctc gccgaaaaag gtggcaaagg
     1501 aaacaggcga gcgcatcgcc aaaattatgg aagagatcaa ttatatccct aatcgcgctc
     1561 ccggcatgtt gttgaatgca caaagttata ccctcggtat cttgattccg tcatttcaaa
     1621 atcaactctt cgctgacatt ctcgcgggaa tagaatccgt cacctctgtg cataattatc
     1681 agacccttat tgctaattac aattacgatc gcgattccga agaagagtcg gtgattaacc
     1741 tgctctctta taacattgac gggattattc tttcggaaaa atatcacacc atcaggacgg
     1801 tgaaatttct gcgttcagcc accattccgg tcgtggaatt gatggatgta cagggagaac
     1861 ggctggatat ggaggtcggt tttgataatc ggcaggctgc ttttgacatg gtgtgtacca
     1921 tgctggataa gcgagtcagg cgcaaaattc tgtatctcgg ttccaaagat gacacccgcg
     1981 atgaacagcg ttatcagggg tattgcgatg cgatgatgct gcataatctt tccccattac
     2041 gcatgaatcc acgcgccatc tcatccattc atttaggaat gcagttaatg cgtgatgccc
     2101 ttagcgcaaa cccggattta gacggtgtat tttgcaccaa tgacgatatt gcgatgggtg
     2161 cgcttctgct gtgccgcgag cgaaacctcg ccgtgccaga acaaatttcc attgcgggtt
     2221 ttcatgggct ggagattggc aggcaaatga tcccaagcct cgccagcgta attactcccc
     2281 gttttgatat tggtcggatg gccgcccaaa tgttgctgag caagattaag aataacgatc
     2341 acaaccacaa cactgttgac ctcgggtatc agatttatca cggtaacacc ctttaggtta
     2401 ttttctcttc attacacata ttctctttgc cggacgttgc ctatactcca ggcaggaaac
     2461 tggaggaaat ctcatgagcg aacccctgtt aattgcccgc acgccggaca cagaactgtt
     2521 tttactgccg ggaatggcta accgtcacgg gctgattact ggcgcaacgg ggacgggtaa
     2581 aactgtcacg ttgcaaaaac tggcagagtc attgtcggaa atcggtgttc cggtgtttat
     2641 ggccgatgtg aaaggcgatc tgaccggcat cgcgcaggca ggaacggcgt cggaaaaact
     2701 gctcgcaaga ctgaaaaata tcggcgtcaa cgactggcaa ccgcatgcca atccggtggt
     2761 ggtgtgggat atctttggcg agaaaggcca tccggtgcgg gcgacggttt cggatctggg
     2821 gccgctgttg ctggcacgac tgttgaatct caacgatgtg caatctggcg tgctgaatat
     2881 tatcttccgc attgcggacg atcagggatt gttgctgctc gactttaaag atttgcgggc
     2941 gattacccag tacatcggcg ataacgccaa atccttccag aatcagtacg gaaatatcag
     3001 tagcgcatcg gttggtgcca tccagcgcgg attactgtcg ctggaacagc aaggcgcaga
     3061 acacttcttt ggtgagccga tgctggatat caaagactgg atgcgcaccg atgccaacgg
     3121 taaaggcgtt atcaatatcc tcagcgccga gaaactttat cagatgccga aactgtacgc
     3181 cgccagcctg ctgtggatgc tttcagagtt atatgaacaa ttgcctgaag cgggcgatct
     3241 ggagaaacca aaactggtgt ttttcttcga cgaagcacat ctgctgttta acgatgcgcc
     3301 acaggtactg ctggataaga ttgagcaggt gataaggctt attcgctcaa aaggcgtggg
     3361 cgtctggttc gtttcgcaaa acccgtcaga tatcccggat aacgtgctag ggcagctcgg
     3421 taatcgcgtt caacacgctt tgcgtgcttt tacgcctaaa gatcagaaag cagtgaaggc
     3481 agcggcgcaa accatgcggg ccaatccggc gtttgatacc gaaaaggcaa tccaggaact
     3541 ggggaccggc gaggcgttaa tctcgtttct cgatgcgaaa ggaagtcctt cagtggtgga
     3601 acgggcgatg gtgatcgcac cttgttcgcg aatggggccg gtgacggaag atgagcgtaa
     3661 tggcctgatt aatcactccc cggtgtatgg caaatatgaa gatgaggtgg accgcgagtc
     3721 cgcctatgag atgctgcaaa aaggctttca ggccagtacc gagcagcaaa ataatccccc
     3781 cgtgaaaggg aaagaggtgg cggtggatga cggtattctt ggtggattga aggatatttt
     3841 gtttggcact accggaccac gcggcgggaa gaaagatggt gtggtgcaaa caatggcgaa
     3901 aagcgccgct cgccaggtga cgaatcagat tgtacgcggg atgttgggga gtttgctggg
     3961 ggggagaaaa aggtaagtca gacgtgatct gtgcccctca ccctaaccct ctccccaaag
     4021 gggcgagggg accgttcgaa ctcgtatttg actttgtcaa caataataaa gggagctttc
     4081 gctcccttgt tagattacga ttttctcatt aacagccaca ggctgattaa gaagaagctg
     4141 gcgcttggca acagtgcgcc gatgatcggc gggatgccat aaaccaacgt cagcgggccg
     4201 aagatctggt ccagtacgta gaagacaaaa ccgaagctga taccggtgac cacacgcacg
     4261 cccatcggta cgctacgcag tgggccaaag atgaacgaca gcgccatcaa catcatcacc
     4321 gccacggata gcggctggaa gattttgctc cacatgttga gctgataacg tccggcatcc
     4381 tgaccgctcg acttcagata cttcacatag ttgtgcaaac cactgatgga gagtgcatcc
     4441 gggtccagcg ccaccacgcc cagtttgtct ggcgtgaggt tggttttcca ggtgccgctc
     4501 accgtctgcg aaccggtgat ctgtttcgga ttggtcagat cagattcatc aacctgcgac
     4561 agacgccaga ctttatgttc cgggtcaaac ttcgcggtag cggcataacg tacggattgc
     4621 agacgacgat tctcgttaaa ggcataaata ctgatgccac ctaactcttc gtcaccttta
     4681 acccgctcaa tgtagacgaa gttgttgcca tctttcgccc ataagccttg ctgagtggag
     4741 agcaacgagc cgccgtacat cgcctgcgca cggtagttac gcgccatctg ctcgccctgc
     4801 gacgcgaccc attcaccaat cgccatcgtc agcaagacca gcggaatggc ggttttcatc
     4861 accgacagcg ccacctgcat acgggtaaaa ccagaagcct gcatcaccac cagttcgctg
     4921 cgctgcgcca gcatcccaag accaagcaac gccccaagca gagccgccat cgggaagaag
     4981 atttgcacat ctttcggcac gctcagcaag gtatacattc ctgcgcctaa cgcgtcgtaa
     5041 ctcccctgcc cggctttttt cagctgatcg acaaacttga taatgcccga cagcgacacc
     5101 agcatgaaca gcgtcatcat gatggtggtg aaaatagttt taccgatata gcggtcaagt
     5161 acgccaaaag gttgcatcac accgctcctt tacgcgaaaa actggcgcgc aggcggcgga
     5221 ccggcacggt gtcccaaagg ttgagaacaa tcgccaaagc cagataaatc aggttaacgg
     5281 tccacatcca cagcgtcggg tccagcttac ctttaccgcc gttcgatttc agggaggtct
     5341 ggatcaggaa gaaaagtaga tacagcagca tggctggcag catcgacagt acgcgtccct
     5401 gacgcgggtt aaccacgctc aacggtacaa ccataagtgc catcataaac acggtgaata
     5461 ccaacgtgat acgccagttc agttctgcgc gggcacgatc ggtgtcagtg ttccacaatg
     5521 tgcgcatgtc catctggtcg gtatcgttcg ggtcgagcac caccgcctgg tgaccaatga
     5581 tcgcctgata atcctgaaag tccgtaatgc ggaaatcgcg taacagtgca gtgccttcga
     5641 agcgcgttcc ctggttgaga gtgacgacct gggagccgtc gcgcagctgg gttaaatgtc
     5701 cggaatcggc caccaccaca gaaggacgtg cattaccttt tggtcgaatt tgtgcgagga
     5761 acacatcttt gaaatcgctg ccgtcaacgc tttcgatgaa cagcaccgag ctgccattag
     5821 tcgcttgctg gaattgccct tgcgccagcg ccgccatgcc agggttcgct ttcgcttctg
     5881 ctaacacttc atcctgatga cgcgatgacc atggtcccgc ccacatcacg ttaaccgccg
     5941 cgacgattgc cgtgaacacc gcaaggatca ttgccgcttt caccagcacc gctttgctca
     6001 gaccgcaggc atgcattacc gtaatttcac tttcggtata cagtttgccc agcgtcatca
     6061 gcagcccgag gaacaggctt aatggcagga taagctgcgc catttccggc acgcccaacc
     6121 cgagaaggga gagcaccaga ttcgccggaa tatcgccgtc aaccgctgcg ccgaggatcc
     6181 tcactaactt ttgacagaag aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt
     6241 tgagcgtctc ccgcaccaga tatcttatga ttatcacttt aaatacgccc gtaaaaactc
     6301 gtcttttgca ggattttagc ttgtttcatg gcttaaacgt catttattct cttgagtcgt
     6361 cgaaatcgtc gctaagataa ttatactcaa cggattcacc tctcagattt tgttctgacg
     6421 tgccaatgcc gtaataacgt taagattaac acgaagtcat cgcaacagcg gacatgagtt
     6481 acgaaagctt gcaattctat ctgtagccac cgccgttgtc tttaagattc aggagcgtag
     6541 tgcatggagt ttagtgtaaa aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc
     6601 gtgggcgtct tcgaaccacg tcgcctttct ccgattgcag aacagctcga taaaatcagc
     6661 gatgggtaca tcagcgccct gctacgtcgg ggcgaactgg aaggaaaacc ggggcagaca
     6721 ctgttgctgc accatgttcc gaatgtactt tccgagcgaa ttctccttat tggttgcggc
     6781 aaagaacgtg agctggatga acgtcagtac aagcaggtta ttcagaaaac cattaatacg
     6841 ctgaatgata ctggctcaat ggaagcggtc tgctttctga ctgaactgca cgttaaaggc
     6901 cgtaacaact actggaaagt gcgtcaggct gtcgagactg caaaagagac gctctacagt
     6961 ttcgatcagc tgaaaacgaa caagagcgaa ccgcgtcgtc cgctgcgtaa aatggtgttc
     7021 aacgtgccga cccgccgtga actgaccagc ggtgagcgcg cgatccagca cggtctggcg
     7081 attgccgccg ggattaaagc agcaaaagat ctcggcaata tgccgccgaa tatctgtaac
     7141 gccgcttacc tcgcttcaca agcgcgccag ctggctgaca gctacagcaa gaatgttatc
     7201 acccgcgtta tcggcgaaca gcagatgaaa gagctgggga tgcactctta tctggcggtc
     7261 ggtcagggtt cgcaaaacga atcgctgatg tcggtgattg agtacaaagg caacgcgtcg
     7321 gaagatgcac gcccaatcgt gctggtgggt aaaggtttaa ccttcgactc cggcggtatc
     7381 tccatcaagc cttcagaagg catggatgag atgaagtacg atatgtgcgg tgcggcagcg
     7441 gtttacggcg tgatgcggat ggtcgcagag ctacaactgc ctattaacgt tattggcgtg
     7501 ctggcaggct gtgaaaacat gcctggcggg cgtgcctatc gtccgggcga tgtgttaacc
     7561 accatgtccg gtcaaaccgt tgaagtgctg aacaccgatg ccgaaggccg cctggtactg
     7621 tgcgacgtgt taacttacgt tgaacgtttt gagccggaag cggtgattga tgtggcgacg
     7681 ctgaccggtg cctgcgtgat cgcgctgggt catcatatta ctggtctgat ggcgaaccat
     7741 aatccgctgg cccatgaact gattgccgcg tctgaacaat ccggtgaccg cgcatggcgc
     7801 ttaccgctgg gtgacgagta tcaggaacaa ctggagtcca attttgccga tatggcgaac
     7861 attggcggtc gtcctggtgg ggcgattacc gcaggttgct tcctgtcacg ctttacccgt
     7921 aagtacaact gggcgcacct ggatattgca ggaaccgcct ggcgttctgg taaagcaaaa
     7981 ggcgcaaccg gtcgtccggt agcgttgctg gcacagttcc tgttaaaccg cgctgggttt
     8041 aacggcgaag agtaagtttg cgttttaccc tcaccctaac cctctcccta agggagaggg
     8101 aaccgatcga gcgcaataga aattcattgc tcatctttca gggtgaggaa tgcagcaata
     8161 tttaaatcca ccacaagaag ccccatatat gaaaaacgca acgttctacc ttctggacaa
     8221 tgacaccacc gtcgatggct taagcgccgt tgagcaactg gtgtgtgaaa ttgccgcaga
     8281 acgttggcgc agcggtaagc gcgtgctcat cgcctgtgaa gatgaaaagc aggcttaccg
     8341 gctggatgaa gccctgtggg cgcgtccggc agaaagcttt gtcccgcata atttagcggg
     8401 agaaggaccg cgcggcggtg cgccggtgga gatcgcctgg ccgcaaaaac gcagcagcag
     8461 cccgcgcgat atattgatta gtctgcgaac aagctttgca gattttgcca ctgctttcac
     8521 agaagtggta gacttcgttc cttatgaaga ttctctgaaa caactggcgc gcgaacgcta
     8581 taaagcctac cgcgtggctg gtttcaacct gaatacggca acctggaaat aatggaaaag
     8641 acatataacc cacaagatat cgaacagccg ctttacgagc actgggaaaa gcagggctac
     8701 tttaagccta atggcgatga aagccaggaa agtttctgca tcatgatccc gccgccgaac
     8761 gtcaccggca gtttgcatat gggtcacgcc ttccagcaaa ccatcatgga taccatgatc
     8821 cgctatcagc gcatgcaggg caaaaacacc ctatggcagg tcggtactga ccacgccggg
     8881 atcgctaccc agatggtcgt tgagcgcaag attgccgcag aagaaggtaa aacccgtcac
     8941 gactacggcc gcgaagcttt catcgacaaa atctgggaat ggaaagcgga gtccggcggc
     9001 accattaccc gtcagatgcg ccgtctcggc aactctgtcg actgggagcg tgaacgcttc
     9061 acgatggacg aaggcctgtc caatgcggtg aaagaagttt tcgttcgtct gtataaagaa
     9121 gacctgattt accgtggcaa acgcctggta aactgggatc cgaaactgcg caccgctatc
     9181 tctgacctgg aagtggaaaa ccgcgaatcg aaaggttcga tgtggcacat ccgctatccg
     9241 ctggctgacg gcgcgaaaac cgcagacggt aaagattatc tggtggttgc gactacccgt
     9301 ccggaaaccc tgttgggcga taccggcgtg gccgttaacc cggaagatcc gcgttacaaa
     9361 gacctgattg gcaaatatgt cattctaccg ctggttaacc gtcgtattcc gatcgttggc
     9421 gacgaacacg ccgacatgga aaaaggcacc ggctgcgtga aaatcactcc ggcgcacgac
     9481 tttaacgact atgaagtggg taaacgtcac gcgctgccga tgatcaacat cctgaccttt
     9541 gacggcgata tccgtgaaag cgcccaggtg ttcgatacca aaggtaacga atctgacgtt
     9601 tattccagcg aaatcccggc agagttccag aaactcgagc gttttgctgc acgtaaagca
     9661 gtcgttgctg cggttgacgc actcggcctg ctggaagaaa ttaaaccgca cgacctgacc
     9721 gttccttacg gcgaccgtgg cggcgtagtt atcgaaccaa tgctgaccga ccagtggtac
     9781 gtgcgtgccg atgtactggc gaaaccggcg gttgaagcgg ttgagaacgg cgacattcag
     9841 ttcgtaccga agcagtacga aaacatgtac ttctcctgga tgcgcgatat tcaggactgg
     9901 tgtatctctc gtcagctgtg gtggggtcac cgtatccctg catggtacga cgaagcaggt
     9961 aacgtttatg ttggccgcaa cgaagaagaa gtgcgcaagg aaaataacct cggtgccgac
    10021 gttgccctgc gtcaggacga agacgttctc gacacctggt tctcttctgc gctgtggacc
    10081 ttctctaccc taggctggcc ggaaaacact gacgcactgc gccagttcca cccaaccagc
    10141 gtgatggtat ccggcttcga catcattttc ttctggattg cccgcatgat catgatgacc
    10201 atgcacttca tcaaagatga aaatggcaaa ccgcaggtgc cgttccacac cgtttacatg
    10261 accggcctga ttcgtgacga cgaaggccag aagatgtcca aatccaaggg taacgttatt
    10321 gacccgctgg atatggttga cggtatttcg ctgccggaac tgctggagaa acgtaccggc
    10381 aatatgatgc agccgcagct ggcggacaaa atccgtaagc gcaccgagaa gcagttcccg
    10441 aacggtattg agccgcacgg caccgatgcc ctgcgcttca ccctggcggc gctggcgtct
    10501 accggtcgtg acatcaactg ggatatgaag cgcctggaag gttaccgtaa cttctgtaac
    10561 aagctgtgga acgccagccg ctttgtgctg atgaacacgg aaggtcagga ttgcggcttc
    10621 aacggtggtg aaatgacgct gtcgctggcg gaccgctgga ttctggcaga gttcaaccag
    10681 accatcaaag cgtaccgcga agcgctggac agcttccgct tcgatatcgc tgcaggcatt
    10741 ctgtatgagt tcacctggaa ccagttctgt gactggtatc tggagctgac taagccggta
    10801 atgaacggtg gcaccgaagc cgaactgcgc ggtactcgcc atacgctggt gaccgtactg
    10861 gaaggtctgc tgcgcctcgc gcatccgatc attccgttca tcaccgaaac catctggcag
    10921 cgtgtgaaag tactttgcgg tatcactgcc gacaccatca tgctgcagcc gttcccgcag
    10981 tacgatgcat ctcaggttga tgaagccgca ctggccgaca ccgaatggct gaaacaggcg
    11041 atcgttgcgg tacgtaacat ccgtgcagaa atgaatatcg cgccgggcaa accgctggag
    11101 ctgctgctgc gtggttgcag cgcggatgca gaacgtcgcg taaatgaaaa ccgtggcttc
    11161 ctgcaaaccc tggcgcgtct ggaaagcatc accgtgctgc ctgccgatga caaaggtccg
    11221 gtttccgtta cgaagatcat cgacggcgca gagctgctga tcccgatggc tggcctcatc
    11281 aacaaagaag atgagctggc gcgtctggcg aaagaagtgg cgaagatcga aggcgaaatc
    11341 agccgtatcg agaacaagct ggcgaatgaa ggctttgtcg cccgcgcacc ggaagcggtc
    11401 atcgcgaaag agcgtgagaa gctggaaggc tatgcggaag cgaaagcgaa actgattgaa
    11461 cagcaggctg ttatcgccgc gctgtaatca aaacacagtg atgaaaacga aggccggagc
    11521 atgctccggc cttttttatc tcttacagaa atggtaaaga aggcataatt ccccgtgaaa
    11581 tactggctaa gaatccttta tctggttgtt cttctttatt gattaaagga aggtcatcca
    11641 aatcgcccag caaatacgta ttttttgcct gccagtcaat catccatatt ttcagcagtg
    11701 gataagccag accgcccgta atcccggata tcaccaccaa cgcacacatg cgataaagca
    11761 taccgtggta tgttaaagtt gagcgaaaac gaatacgccc atcattcagt gacaggttgc
    11821 tcataaaatg atttcgcaac gacaccgtta aataactggt gctaacagca atcccaaaat
    11881 aatagattaa ctgcgcgatt atcattttac gttgcatttc cataaattgc tgtaaattct
    11941 caatatcatc atttgcatac ccagaactat catactcatt taatatttga tcgaagataa
    12001 tataaccagc aacagcgaga aaaggtaata aagccaaaaa tgcgagaatg gcatatttta
    12061 tacaatatgt agcatctaat ttcaccttga aacgatgtat accgaaactg gtattactcc
    12121 agagaaaact cattaccaga ctatataaag tgccattaaa aataccaatg aaaacaatac
    12181 aaactattgt catcagaacc acagatataa taacgctact tgaactattg gcatgtagca
    12241 tctttgtcga gataaagaaa acagtcccca tcccaagggc cattaaaatc ggcaagaaaa
    12301 aggtcaccca ccagaaccct ttcatagagc aattaaaact aaatcttacg ccgttgagac
    12361 tggtcatcaa ggcctgataa cgcagtcctt ttgctgccat aaatataagc aaaaccaaca
    12421 gtgacaaagt caaaacacag ccaataagcg gcatatctgc tgacactgtc attaagattg
    12481 cgaaatagaa aaaaacaaaa acaagacaac taacaaaaac attcccaccg gtaatcccat
    12541 aagaaaatcg ttgtccgtta acttccatat tagcatagag ataacgctta catttcatta
    12601 atgcccatgg taaatagata cctagcgtta taatcgttaa caacacattc accacacaaa
    12661 taagaaaata tctctcacct gtaccatgaa aaacaaacga atgggacgga
//
LOCUS       DAZOYL010000105        12494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZOYL010000105 DAZOYL010000000
VERSION     DAZOYL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.105"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1863)
                     /gene="deaD"
                     /locus_tag="ACHUOY_002125"
     CDS             complement(<1..1863)
                     /gene="deaD"
                     /locus_tag="ACHUOY_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR0682902.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTG"
     gene            complement(1856..1936)
                     /gene="yrbN"
                     /locus_tag="ACHUOY_002126"
     CDS             complement(1856..1936)
                     /gene="yrbN"
                     /locus_tag="ACHUOY_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR0682903.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(2043..2927)
                     /gene="nlpI"
                     /locus_tag="ACHUOY_002127"
     CDS             complement(2043..2927)
                     /gene="nlpI"
                     /locus_tag="ACHUOY_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR0682904.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(3036..5171)
                     /gene="pnp"
                     /locus_tag="ACHUOY_002128"
     CDS             complement(3036..5171)
                     /gene="pnp"
                     /locus_tag="ACHUOY_002128"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR0682905.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(5418..5687)
                     /gene="rpsO"
                     /locus_tag="ACHUOY_002129"
     CDS             complement(5418..5687)
                     /gene="rpsO"
                     /locus_tag="ACHUOY_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR0682906.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(5836..6780)
                     /gene="truB"
                     /locus_tag="ACHUOY_002130"
     CDS             complement(5836..6780)
                     /gene="truB"
                     /locus_tag="ACHUOY_002130"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR0682907.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(6780..7181)
                     /gene="rbfA"
                     /locus_tag="ACHUOY_002131"
     CDS             complement(6780..7181)
                     /gene="rbfA"
                     /locus_tag="ACHUOY_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR0682908.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(7345..10017)
                     /gene="infB"
                     /locus_tag="ACHUOY_002132"
     CDS             complement(7345..10017)
                     /gene="infB"
                     /locus_tag="ACHUOY_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR0682909.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(10042..11529)
                     /gene="nusA"
                     /locus_tag="ACHUOY_002133"
     CDS             complement(10042..11529)
                     /gene="nusA"
                     /locus_tag="ACHUOY_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR0682910.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(11557..12009)
                     /gene="rimP"
                     /locus_tag="ACHUOY_002134"
     CDS             complement(11557..12009)
                     /gene="rimP"
                     /locus_tag="ACHUOY_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR0682911.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(12216..12292)
                     /locus_tag="ACHUOY_002135"
     tRNA            complement(12216..12292)
                     /locus_tag="ACHUOY_002135"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12256..12258),aa:Met,seq:cat)
BASE COUNT         2818 a         3512 c         3029 g         3135 t
ORIGIN      
        1 accggtagag tcatcacgac gcggagcgcg gccttcacga cgttcgccgc taaaacgacg
       61 accatcacca cggccacctt cacggcgttc accgctgaag ttacgaccgc cttcacgacg
      121 ttcgccaccg aaaccacgac caccgccacg acgctcaccg ccagtatgcg gctgtgcatc
      181 gcccagtaac tgcatgttca tcggcttgtt gagaatgcga gtgcgcgtaa agtgttgcag
      241 cacttcaccc ggcatacctt tcggcagttc gatggtggag tgagaagcaa acagcttgat
      301 gttaccaatg taacggctac tgatgtcgcc ttcgttagca atcgcaccaa cgatatgacg
      361 aacttcaaca ccatcatcgc ggcccacttc aatgcggtac agctgcatat cgccaacatc
      421 acgacgttca cgacgcggac gatcttcacg gtcaccacgc gggccacggt cgttacgatc
      481 gcgcggacca cggtcatcac ggtcacggaa ttcacgtttc ggacgcatcg gcgcatctgg
      541 cggtacgatc agagtacgtt caccctgtgc cattttcagc agtgccgcag ccagagtttc
      601 gagatccagc tcttcacctt ctgcagtcgg ctgaattttg ctcagcagtg cgcggtattg
      661 atccagatcg ctgctttcca gctgctgctg tactttagcg gcgaattttt ccagacggcg
      721 tttgcccagc agttctgcgt tcggcagttc tacttccgga atagtcagct tcatagtacg
      781 ttcaatgttg cgcagcagac gacgctcgcg gttctcaacg aacagcagcg cgcggccagc
      841 acgacccgca cgaccggtac gaccgatacg gtgaacgtaa gactcagaat ccatcgggat
      901 atcgtagtta actaccaggc tgatacgctc aacgtccagg ccacgggctg caacgtcggt
      961 cgcaatcagg atgtccagac gaccatcttt caggcgttcc agtgtctgtt cacgcagcgc
     1021 ctggttcatg tcaccgttca gcgcggcgct gttgtagccg ttacgctcaa gagcttcagc
     1081 cacttccaga gtcgcgtttt tggtacgaac gaagataatc gccgcatcaa aatcttccgc
     1141 ttccaggaaa cgtaccagtg cttcgttttt gcgcataccc cagacagtcc agtagctctg
     1201 gctgatgtca ggacgggtag tcacgctgga ctgaatgcgc acttcctgcg gctctttcat
     1261 aaagcggcgg gtaatgcgac gaatcgcttc cggcatggtt gcagagaaca gagcggtctg
     1321 atgaccttcc gggatctgcg ccataatggt ttcaacgtct tcgatgaagc ccatgcgcag
     1381 catttcgtca gcttcatcca gaaccagacc gctcagttta gagaggtcca gagtgccacg
     1441 tttcaggtgg tccagcagac ggcccggagt accgacaacg atctgcggcc cctgacgcag
     1501 ggcgcgtaat tgcacgtcat aacgctggcc gccgtacaga gcaaccacat ttacgccgcg
     1561 catgtgttta gagaaatccg tcattgcttc agcaacctgt accgccagtt cgcgggtcgg
     1621 tgccagcacc agaatctgtg gtgctttcag ctcaggatca agattctgca acagaggtaa
     1681 agagaatgct gcagtttttc cgctccccgt ctgggccata cccagaacgt cgcggccatt
     1741 cagcagatgt ggaatacact ctgcctgaat tggagatggt ttttcgtaac ccagatcgtt
     1801 aagggcttca aggataggag ccttcaggcc cagatctgca aaagtggttt cgaattcagc
     1861 catgtagtac gtgtgcctca aaattaatgg cggccagtct acataactca tcatgaaatt
     1921 gatcagcaat tttcattgaa aagtgtgaac cggctcaaag taggtgtatt aacgaacaac
     1981 aacgccctca cccgttaagg tgatggcaat caaaaaagat tacgggctga tgtgtacgtc
     2041 agctattgct ggtccgattc tgccaggtca tcttggtcct ggcccaggag cgataattcc
     2101 aacaatgcgt atcggtgctc aacaaagtta tgaacgttgt tggcaaccgc cagtttgaac
     2161 agtgccgtgg cgctgtccaa atcccccaga cttaggtagt acttacctaa atagaagttg
     2221 gtttcactga gatgctcagc gagcgaggtg ttatccgttg cgtccgcctt gagcctttcc
     2281 attaacgttt gttcgctaat gttgcccaag tagaactcga caatgttcca tccccactgt
     2341 tccttatccg atttttcgaa gtgctgtttc aacacttctt tagcctgctt ctcatcgagc
     2401 ttctgctcgg cgagataaag ccacagacta cggaaaggat cattgggatc gtcttgataa
     2461 aacgccagca gatcatcttg cgctaacttg tcacgaccgc cgtaatataa tgcgatcccg
     2521 cgattcaagt gcgcgtagtt gtaagttgga tcaagctcaa gtacagaatc aaacgcttca
     2581 taggcagcat caaaattgcc tgcctgcgtt aaatatatgc ctaagtaatt gaatacttca
     2641 ggcatatccg gtcggattgc cagcgcttgc gaaaaatcgt tacgcgctaa tgccctcaga
     2701 ccgagactat catacaacac tccgcgctca tataaaagct gtgcgcgttc gtcatcggtt
     2761 aaagcccgac tggcaaggat ttgttccata cgtgccagaa tcacttcctg ctgtaaagtc
     2821 ggttgcaatg gtaccgcgag gacttcactt ttacgccagg aagtattact gcatcctgca
     2881 agcgtaagtg ctgtcgcaac gaaacaccag cgcaaaaaag gcttcatttc ccactcccga
     2941 agaccacggt tgaatgaacg tcctgttccc ggttgctaac aaggcgtcct gcccggttaa
     3001 aagccccccg ccgcagcgga gggcaaatgg caaccttact cgccctgttc agcagccgga
     3061 gcttccggtg ctgcagcagg ttgagactgc tcagtcgctt ctttaatgct cagacggata
     3121 cggccctggc gatcaacttc tagaactttc accggtactt cctgacccat ctgcaggtaa
     3181 tcggtcactt tctcaacgcg tttgtcagcg atttgagaga tgtggaccag accttcttta
     3241 ccgccgccga tggcaacaaa tgcgccaaag tcaacgatac gggtcacttt accagtgtag
     3301 acgcggccca cttcgatttc tgcagtgatc tcttcgatac gacgaatagc atgtttcgct
     3361 ttctcgccgt cggtcgctgc gatcttcaca gtaccgtcat cttcgatttc gatggtagtg
     3421 ccagtttctt cggtcagggc acggataaca gagccgcctt taccgataac gtctttgatc
     3481 ttatccgggt tgatcttgat ggtatggata cgcggtgcga actcagagat atcgccacgc
     3541 ggcgcgttga tcgcctgttc cattacgccc aggatatgca gacgcgcacc tttagcctgg
     3601 ttcagcgcaa cctgcatgat ctctttggtg ataccttcaa ttttgatatc catctgcagt
     3661 gcagagatac cgtcgcggga acctgcaact ttgaagtcca tatcgcccag gtgatcttcg
     3721 tcgcccaaaa tgtcagacag tacaacgtag ttgtcgcctt ctttcaccag acccattgcg
     3781 atacccgcaa cggcagcttt gattggcaca cctgcgtcca tcagcgccag agacgcgccg
     3841 cacacggaag ccatagaaga agaaccgttg gattcggtga tttcagacac tacacgtacg
     3901 gtgtacggga atttgtccat atccggcatg actgccagca cgccgcgctt cgccagacga
     3961 ccgtgaccaa tttcacgacg cttcggagaa ccgaccatgc cggtttcgcc tacggagtac
     4021 ggagggaagt tgtagtggaa caggaaggta tcggtacgtt cgcccatcag ttcatcaaga
     4081 acctgcgcgt cacgagcggt acccagcgtt gcagtaacca gtgcctgcgt ttcaccgcgg
     4141 gtgaacagcg cagaaccgtg agtacgcggc agcacgccag tacgcacatc cagaccacgg
     4201 atcatatctt tttcacgacc gtcgatacgc ggttcgcctg ccagtacgcg gctacgaaca
     4261 acgtttttct cgatagcgtg cagaatttca cccagttcgt tttcgtccag ggtttcgtct
     4321 tcagcaagca gcgtcgcgat ggtttcagat ttgatgacat caacctgcgc ataacgctct
     4381 tgtttgtcgg tgatgcggta agcatcactc agacgagctt cagccagtgc agcaacgcgc
     4441 gcgttcagcg cttcgtttac cggctccggc tgccagtccc aacgcggttt accggcttct
     4501 ttcactagtt cattgatgtt ctgaataaca acctgctgtt gttcatgacc gaacactact
     4561 gcgcccagca tctgatcttc gctcagcagt tcagcttcag attcaaccat cagtacagcg
     4621 gcttcagtac cggcaacaac cagatccagt ttgctctctt tcagctcgtc ctgagtcggg
     4681 ttcagtacgt actggtcatt gatgtaacct acgcgggcag caccaatcgg gccattgaac
     4741 ggaataccag acagagacag tgctgcggaa gcaccaatca tcgcgacgat atccgggtta
     4801 acctgcgggt taacagaaac cacggtggcg ataacctgaa cttcgttgac gaagccttcc
     4861 gggaacagcg ggcgaatcgg gcggtcaatc agacgcgcga tcagggtttc gccttcgctt
     4921 ggacgacctt cacgacggaa gaagctaccc gggatacgac cagcagcgta ggtacgctcc
     4981 tgatagttaa cggtcagtgg gaagaagtcc tgacctggtt tggctttttt ctgtccaaca
     5041 acggtaacga ataccgcggt gtcatccatg ctaaccataa cagcggcagt agcctgacga
     5101 gccatcatgc cggtttccag agtcacggtg tgttggccgt actggaattt acgaacgatc
     5161 ggattaagca atgtaatatc ctttctcttt cttagacagt accttacggc actggtgtta
     5221 atacccgatc ttctgcgcat cctcgcgact aatgacaacc ctaacccagc tctatgtggg
     5281 taaagcctct cattagccgc gcgaacctct gcaacggaag atcattcata gcaacaatac
     5341 attagtttcc agtgaattgc tgccgtcagc ttgaaaaaag gggccactca ggcccccttt
     5401 tctgaaactc gcaagaatta gcgacgcaga cccaggcgct cgatgagctg ggtgtaacgt
     5461 gctacgtctt tacgtttcag gtagtcgagc agtttacgac gctgagaaac catgcgcagc
     5521 agaccacgac ggctgtggtg atcttttttg tgctctgcaa agtggccctg caggtggttg
     5581 atctgtgcag tcagcagtgc tacctgaact tcggtagaac cggtgtcgtt tgcgtcacga
     5641 ccaaactcag aaacgatttt agctgttgct tcagtactta gagacatttt aaaactccaa
     5701 agtatataga atgaaaggac gccgatctct aattcagcga tcccagtgta cgttacgcaa
     5761 agtgttaaac aatttacgcg acgttaagcg gcagtattct actcgtagcg acctgttatc
     5821 gcaagacggt taacattacg ccgggtattc aaccaccagg cgacgaggcg cgacgcggcc
     5881 ttcatcgtca atttcgccca taccgataaa cttgccgttc tcaccttctg taacgcgaac
     5941 cagtccttcc agcggcgcac cggatgtacg aactgggtta ccatttttga agtaaacaga
     6001 agacgttaac ggaagattca ccaccgggta gtccgaagct ggactgtcca ttggcatcag
     6061 taatggatca agtaactccg cagccggaat atcctgctgt tcggcttgtt cgaccagttc
     6121 acgcagatgt tccagggtca ccatccgttc aaccggatat ttacttaccg ccagacgacg
     6181 caggtaaata acatgcgcgc cacaaccgag tttttcaccc aggtcatcaa tgatggtgcg
     6241 gatataagtg ccttttgagc agtgaatttc cagctccagc tcattgcctt cgtggcgaat
     6301 aaacagcaat tcataaacgg taatcgggcg cgcttcacgc ggtacttcaa tgccctgacg
     6361 cgcatattcg tacagttttt taccctgata tttcagtgct gaatacatcg aagggatctg
     6421 ttcgatatcg ccacggaaag tatccagcgc cgccgccagt tgctctgcgc taaaggttac
     6481 cggacgttct tcaacgattt gtccgtcggc atcagaagta tcggtacgct gtccaagacg
     6541 cgcaatgacc cgatagcgtt tgtcggagtc cagcagatac tgggaaaact tcgtcgcttc
     6601 cccgaggcaa attggcaaca tgcctgttgc cagcgggtcc agcgcaccgg tatgcccggc
     6661 acggttggcg ttataaatcc gtttcacttt ttgcagcgca tcattgctgg acataccctg
     6721 cggtttatcc agcaacaaaa cgccgttaat gtcgcgaccg cgacgacgag gacgactcat
     6781 tagtcctcct tgctgtcgtc cgggttaaca cgacgttctt cgtcatgttt gaccacgctg
     6841 gtcaccaggt ttgacatgcg catcccttca accagagagt tgtcgtagaa gaaggtcagt
     6901 tccggcacga tacgcaggcg catcgctttc cccagcaggc tgcggatgaa accagaagct
     6961 tcttgcaacg ctttgatgcc cgctttaacc gcgtcttcat ctttgtcgtt gaggaacgtc
     7021 acatatactt tggcatacgc caggtcgcga gacatttcga caccggaaac ggtggtcatc
     7081 atgcccaggc gaggatcttt aatttcacgc tgcaggatga gagcgatctc tttttgcatt
     7141 tcctgcgcta cgcgctgcgg gcgaccaaat tctttcgcca taataaattc tcctgacaaa
     7201 aaaggggctg ttagcccctt tttaaaatta atttcaggtg gaagggctgt tcacgttggc
     7261 ctgataagac gcgccagcgt cacatcaggc aatccatgcc ggatgcggcg taaacgcctt
     7321 atcccgcatg gaaccctaaa aaccttaagc gatggtacgt tggatctcga tgatttcgaa
     7381 tacttcgatc acatcgccag tgcggacgtc gttgtagttc ttaacgccga taccacattc
     7441 cataccgtta cggacttcgt taacgtcatc tttgaagcgg cgcagggact ccagctcgcc
     7501 ttcgtagata accacgttgt cacgcagaac gcggatcggg ttgtgacgtt taaccacacc
     7561 ttcggtaacc atacagcctg cgatagcacc aaatttcggc gatttgaaca cgtcacgaac
     7621 ttccgccaga ccgataatct gctgtttcag ttccggagac agcataccgc tcatcgccgc
     7681 tttcacttcg tcaatcaggt tatagatgac ggagtagtaa cgcagatcca ggctttccgc
     7741 ttcaatcact ttacgtgcag aggcatcagc acgtacgttg aagccaacca ggatggcgtt
     7801 ggacgccgca gccagggtgg cgtcggtttc ggtgatacca cctacgccag aaccgatgat
     7861 cttcacttta acttcgtcag tagacagttt cagcaaggag tcggagatcg cttccacaga
     7921 accctgtacg tctgccttca ggacgatgtt cacttcgtga acttcgcctt cggtcatgtt
     7981 ggcgaacatg ttctcgagtt tagatttctg ctgacgcgcc agtttaactt cgcggaattt
     8041 accctgacga tacagtgcaa cttcacgcgc tttcttctcg tcacgtacaa cggtaacttc
     8101 atcacccgca gccggtacgc cggacaggcc gaggatttcc accggaatgg acggacccgc
     8161 ttccagcact tcctgaccca gttcgttacg catcgcacga acacgaccgt attcgaagcc
     8221 acacagaacg atatcgccct tgtgcagagt accttcacgt accagaacgg tagcaaccgg
     8281 accacgacct ttatcgagga aggattcgat aaccgcaccg ctcgccatac ctttacgtac
     8341 cgctttcagc tccagaactt ccgcctgcag caggatagcg tccagcagtt catcgatgcc
     8401 ggtacccgct ttcgcagata cgtgtacgaa ctggctttca ccgccccact cttccggcag
     8461 gatgccatac tgggagagtt cgttcttaac acgatccgga tcggcttctg gtttatcaat
     8521 cttgttcact gcaaccacca ccggtacctg cgccgctttc gcgtgctgga ttgcttcgat
     8581 ggtctgcggc atcacgccgt cgtcggcagc aacaaccagg actacgatgt ccgttgcctg
     8641 cgcaccacgt gcacgcattg aagtaaacgc ggcgtgcccc ggggtgtcca ggaaggtgat
     8701 catgccgttt tcagtttcaa cgtggtatgc accaatgtgc tgggtaatgc cgcccgcttc
     8761 gccagaggca actttcgttg aacgaatgta gtccagcaga gaggttttac cgtggtcaac
     8821 gtgacccatg atggtcacaa ccggcgcgcg cggttcagcc gcagcacccg tgtcacggtc
     8881 gctcattacc gcctcttcca gctcgttttc acgacgcagg ataactttgt ggcccatctc
     8941 ttcagcaacc agctgtgcgg tttcctgatc gataacctgg ttgatggttg ccattgcgcc
     9001 cagtttcatc atcgctttga tgacctgaga gcctttaacc gccatcttgt tcgccagttc
     9061 gccaacggtg atggtttcgc cgatcacaac gtcacggtta acggcctgag ctggcttctg
     9121 gaagccttgc tgcagggaag aacctttacg ttttccgcct ttaccgccac gtactgctgc
     9181 gcgtgcttct tcacgatcag cttttgattc agcgtgtttg ttgcctttct tcggacgcgc
     9241 tgctttcgcg ttacgaccac ggccacgacc gccttcgact tcacgatcgc tttcgtcttc
     9301 tgcctggcga gcatgttgag aagtagtgac gtgataatcg ctggaatctt cagtcggttc
     9361 cgcgttatca gtccatttgt tttcttccgc catacgacgt gcttcttcag caacgcgacg
     9421 tgcttcttct tcgagtttac gatgcgcttc ttcttcggct ttacgcttga gctctgcagc
     9481 ttcctgctca cggcgggctt tttcagcctg ggcgttttta gtcatatcgt cttgttgatt
     9541 gctcactttg tctttttccg cagcttcacg tttcgcttgt tcagcagctt cacgcttagc
     9601 ttgttctgca gcctcacgtt cagctttttg ttgcgcctcg cgtttagccg attcttctgc
     9661 ctcacgacgg gcttgctctt ccgcttcacg ctgcgcttgc tcttccgctg caaggcgttc
     9721 agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga tttgtaccga
     9781 tttgcttttt ccaccggtac caggaatgtt aagggtgctg cgtgttttac gttgcagcgt
     9841 caatttgtcc gggcctgaat ttttctgatt caggtggtca atcaaagtct gtttctcttg
     9901 tgcagacaca gagtcgtcag cagacttccg gatacctgca tcagcaaatt gctgtaccag
     9961 gcgttccacg gaggtctgtc gctctgcggc cagcgtttta atcgttacat ctgtcatgct
    10021 gttccttcct gctacagttt attacgcttc gtcaccgaac cagcaaatat tacgggcagc
    10081 cataatcagt gctccggcct tttcgtcggt caacccttcg atatcagcca gatcatcaat
    10141 gccctgttcg gcgagatctt ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa
    10201 atcacgatct accccttcaa ggttcagcag atcgtcagcc ggtttgttat caccgaggct
    10261 ttcttcctgg gcctgtgcaa tggtggccag tgcattttta gcacgctcgc gcagtgcttc
    10321 aacggtcggc tcatcaaggc cttcgatttc caacagctct ttcatcggca cataggccag
    10381 ttcttccagc gtcgagaagc cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc
    10441 gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct tagcctgcag
    10501 gtcgtcaacg gtcatcacgt tgagttccca accgctcagc tgcgaagcca gacgcacgtt
    10561 ctgaccgtta cggccaatcg cctgcgccag gttaccggct tcaacggcga tatccatggt
    10621 gtgtttatct tcatccacca cgatagaagc aacgtctgcc ggtgccattg cgttaatcac
    10681 gaactgcgcc gggttatcat cccacaggac gatatcgata cgctcgccac ccagttcagt
    10741 agacaccgcc tgaacacgcg cgccacgcat acctacgcaa gcacctaccg gatcgatacg
    10801 tttatcgttg gttttcaccg cgattttcgc acgagaaccc ggatcgcgag ccgctgcttt
    10861 aatttcaatc acttcttcgc cgatttctgg cacttcaata cggaacagtt cgatcagcat
    10921 ttccggcttg gaacgagtga cgaacagttg cgcgccacgc gcttccgggc gaacggaata
    10981 aagcacgcca cgaacgcggt cgccagggcg gaagttttca cgcggcagca tatcttcacg
    11041 caggatcacg gcttcagcgt tgttgcccag atccagagag atgttgtcgc ggtttacttt
    11101 tttcaccacg ccggtgatga tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc
    11161 acgttcggct tcacgcactt tctgcacgat aacctgtttt gccgtctggg tagtgatacg
    11221 gtcaaaggta acagactcaa tctgatcttc aacgtaatcg cccaggttca ggctttcatc
    11281 ttcataacgt gcggcttcaa gggtgatttc cttggtcggc tgggtgactt catcaacaac
    11341 taaccagcga cggaaggtgt caaaatcacc gcttttgcga tcgatctgta cgcggacgtc
    11401 gatctcttgt tcatattttt tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa
    11461 aatcttctcg cgaggtagcg ccttttcatt ggatacggct tcaactacag ccaaaatttc
    11521 tttgttcatc gcgggctttt cacctcatcc agactattaa aagtggggaa ccaggttcgc
    11581 cttctggata ttactcagcg cgaacacttc atctttacct tcgacggtaa ctgtgatcat
    11641 ttcaccgtct accgctttga taacgccctg ccatttacga cggttttgta ccgccatacg
    11701 gagaaccaga gtcacctctt ctccgacaaa acgggcgtag tgttcagccg tgaacagtgg
    11761 gcgatcgaga cccggtgagg agacttccag gttataagca acggtgatgg gatcttcaac
    11821 atccagcaca gcacttacct ggtggctcac atcagcacaa tcatcaacat tgatgccatc
    11881 ttcactatca atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt cgatgccaac
    11941 cagttcaaaa cccagggcct caactggcgc agtaatcatc tctgttaatt tttgctctaa
    12001 tgtggacaag cccaccccca agacataaaa aaagggccta aagcccagtt attctgtagt
    12061 cagataacaa aaaaccccga taaatcgggg ctttatataa ctgaacccta taaccgcaac
    12121 tgcggtctgg agcactttcc agaaggattt tttcaaatcc cactacgaag gccgaagtct
    12181 tcacagtata tttgaaaaag gactctaagg gaaagtggtt gcgggggccg gatttgaacc
    12241 gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg cgcctgaaac
    12301 gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac tgctgggatt tggtgtaccg
    12361 agacgggacg taaaatctgc aggcattata gtgatccacg ccacattttg tcaacgttta
    12421 ttgctaatca tgtgaatgaa tatccagttc actttcattt gttgaatact tttgccttct
    12481 cctgctcccc ctta
//
LOCUS       DAZOYL010000106        12428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZOYL010000106 DAZOYL010000000
VERSION     DAZOYL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.106"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHUOY_002136"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHUOY_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR0682912.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHUOY_002137"
     CDS             433..621
                     /locus_tag="ACHUOY_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR0682913.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHUOY_002138"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHUOY_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR0682914.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHUOY_002139"
     CDS             816..989
                     /locus_tag="ACHUOY_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR0682915.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHUOY_002140"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHUOY_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUOY_002141"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUOY_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR0682916.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHUOY_002142"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHUOY_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR0682917.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUOY_002143"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUOY_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR0682918.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHUOY_002144"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHUOY_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR0682919.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHUOY_002145"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHUOY_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHUOY_002146"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHUOY_002146"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0682920.1"
                     /translation="MFSRELDDEQLTQIASAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUOY_002147"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUOY_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR0682921.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUOY_002148"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUOY_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR0682922.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2992 a         3198 c         3254 g         2984 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacata
//
LOCUS       DAZOYL010000107        12396 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZOYL010000107 DAZOYL010000000
VERSION     DAZOYL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.107"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            38..355
                     /locus_tag="ACHUOY_002149"
     CDS             38..355
                     /locus_tag="ACHUOY_002149"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR0682923.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            424..753
                     /gene="yjdP"
                     /locus_tag="ACHUOY_002150"
     CDS             424..753
                     /gene="yjdP"
                     /locus_tag="ACHUOY_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR0682924.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(900..1658)
                     /gene="phnP"
                     /locus_tag="ACHUOY_002151"
     CDS             complement(900..1658)
                     /gene="phnP"
                     /locus_tag="ACHUOY_002151"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR0682925.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(1660..2094)
                     /gene="phnO"
                     /locus_tag="ACHUOY_002152"
     CDS             complement(1660..2094)
                     /gene="phnO"
                     /locus_tag="ACHUOY_002152"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR0682926.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2081..2638)
                     /gene="phnN"
                     /locus_tag="ACHUOY_002153"
     CDS             complement(2081..2638)
                     /gene="phnN"
                     /locus_tag="ACHUOY_002153"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR0682927.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2638..3774)
                     /gene="phnM"
                     /locus_tag="ACHUOY_002154"
     CDS             complement(2638..3774)
                     /gene="phnM"
                     /locus_tag="ACHUOY_002154"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR0682928.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(3771..4451)
                     /gene="phnL"
                     /locus_tag="ACHUOY_002155"
     CDS             complement(3771..4451)
                     /gene="phnL"
                     /locus_tag="ACHUOY_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR0682929.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            complement(4562..5320)
                     /gene="phnK"
                     /locus_tag="ACHUOY_002156"
     CDS             complement(4562..5320)
                     /gene="phnK"
                     /locus_tag="ACHUOY_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR0682930.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5317..6162)
                     /gene="phnJ"
                     /locus_tag="ACHUOY_002157"
     CDS             complement(5317..6162)
                     /gene="phnJ"
                     /locus_tag="ACHUOY_002157"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR0682931.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(6155..7219)
                     /gene="phnI"
                     /locus_tag="ACHUOY_002158"
     CDS             complement(6155..7219)
                     /gene="phnI"
                     /locus_tag="ACHUOY_002158"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR0682932.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7219..7803)
                     /gene="phnH"
                     /locus_tag="ACHUOY_002159"
     CDS             complement(7219..7803)
                     /gene="phnH"
                     /locus_tag="ACHUOY_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR0682933.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(7800..8252)
                     /gene="phnG"
                     /locus_tag="ACHUOY_002160"
     CDS             complement(7800..8252)
                     /gene="phnG"
                     /locus_tag="ACHUOY_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR0682934.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8253..8978)
                     /gene="phnF"
                     /locus_tag="ACHUOY_002161"
     CDS             complement(8253..8978)
                     /gene="phnF"
                     /locus_tag="ACHUOY_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR0682935.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(8999..9778)
                     /gene="phnE"
                     /locus_tag="ACHUOY_002162"
     CDS             complement(8999..9778)
                     /gene="phnE"
                     /locus_tag="ACHUOY_002162"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR0682936.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(9904..10920)
                     /gene="phnD"
                     /locus_tag="ACHUOY_002163"
     CDS             complement(9904..10920)
                     /gene="phnD"
                     /locus_tag="ACHUOY_002163"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0682937.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(10945..11733)
                     /gene="phnC"
                     /locus_tag="ACHUOY_002164"
     CDS             complement(10945..11733)
                     /gene="phnC"
                     /locus_tag="ACHUOY_002164"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR0682938.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(11866..12309)
                     /gene="yjdN"
                     /locus_tag="ACHUOY_002165"
                     /pseudo
     CDS             complement(11866..12309)
                     /gene="yjdN"
                     /locus_tag="ACHUOY_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
BASE COUNT         2437 a         3526 c         3774 g         2659 t
ORIGIN      
        1 atattgtact tcggcaaata ctgagcacaa tttatcaatg caaccggtgt ttctttttaa
       61 aaaacgtacc ggagcaatgg gcggtcatgt cgctggcacc gcaggattct gcgttgctgt
      121 gccagccttt tattcatggt caatgagtga ggtgattgat aaagacacca ggtcgtcaga
      181 gcgcactgat tatccgcatt atgccagtga agttgcactg gcttttggtt cacgagtggt
      241 tggcctcgaa ctggcaaaaa tgattgtgga tgcgtggctg ggcgcacagt acgaaggcgg
      301 tcgtcatcaa caacgcgtgg aggcgattac ggcaatagag cagcggagaa attgagattc
      361 atccactact tgcatggatg agtaatgact aatgtggata gagtttcttt ttgaggttgg
      421 ctaatgaaac gctttccgct atttctttta ttcaccctgc tcacgctgtc caccgttccg
      481 gcccaggctg atattatcga cgatactatt ggtaatattc agcaggcgat taatgacgct
      541 tataaccctg accgtgggcg agactatgaa gattcgcgcg acgacggctg gcagcgtgaa
      601 gtgagcgacg atcggcgcag acaatatgac gaccgccgcc gccagtttga agatcgccgc
      661 aggcagctgg acgatcgcca gcgccagctt gatcaggagc gtcggcaact ggaggatgaa
      721 gagcggagaa tggaagatga gtatgggcga tgaggttggg tatggagcag gcatcgcccc
      781 tcaccccaat ggggagaggg agaaaacgag cgcaatattc aatattgagc acaatcggtc
      841 ccctcgcccc tctggggaga gggttagggt gagggggcca gcgcgcacat tccctctcat
      901 cacgccaccc caatctccat cccatcaaac cccacctcaa accctgacgg tagtgcgttt
      961 tccatcagcc acgcatcaaa ctggtggctg atatgggtca gaatcacccg tggcgagcgg
     1021 ataacctgat tcagcgcaag cacggtattt aaatcacagt gattacgcgg tgcatccgcg
     1081 cgcggcgggt gactgcaatc catcaccatt acctgcggct gattattgcg taaaaatttc
     1141 agcgtttttt ccggcaagcc agcggtgtca gacagccacg ccacccggct gtgcgccgtt
     1201 tccagtaaat agccgaaagt gagttttgag tggttaagcg gcagcggcgt gacctgcaac
     1261 ccctgcaaat caaacaccac aaacggttcc accgtgtggc tgaaatcaag cagcccggga
     1321 tgtttaaaca gatcgtcgca gccctgttca tccggcgggc cgtagatcgg gatcggatcg
     1381 cccacgcccc agcgcagcgg aaacagcccc tggacgtgat ccatatgata atgcgtcagc
     1441 aaaaactgct ggaacgatcc gggagaccag cgatcggtga gatcgtgcag cccggcgtcg
     1501 atcagggtga tcgcctcgtt aaacttcact acgccgctgc acggttggcg gcgatactgc
     1561 ggcgagcgcc gcgctcggtc gcaggccgcg cactcgcagc cccatgccgg aacgccctgt
     1621 gcgccgccgg tgccggtgag cgtgagggtc agactcatgt tacagcgcct tggtgaagcg
     1681 gaagtgactc tgctcgtagc cttctcgcag atagaaacgg tgcgcgtcgt ggcgcttcac
     1741 gttggtcgaa agctcggtca tttcggcccc ggcctggcgg gcttcttctt ctgcccacgc
     1801 cagcaacttg ctaccgacgt tcaggccgcg cgcctgcggc atcactacca gttcctgaat
     1861 ttcgccgatc cagttgacat gatgcagatg aaactgcaaa tgcaggccga tcatgccgac
     1921 aacctcgcca tccagcagcg ccagatgata gcgcatgttc gggtcgcgca gattggcgct
     1981 aaagcccacg cgaaatgcgt ggtggtcaaa ctccgcctgt tttagctcac aaatcagcgc
     2041 gtaaaccgcg tcggtatcat actgcgtggc cgggcgaagc tcacaagcag gcatggtgtt
     2101 tctccttctg atgaatcagc gtcagcagcg tgtcgaccga ctggcgcagg ctgccgtcat
     2161 tgttgagcgt atggcaatcc tgtggagtat agcgggcggc gcgcgccagg cgggcgttaa
     2221 tttcactggc gttttcacgg ccacggtttt ccaggcgctg gcggaggatc tccggcgaaa
     2281 cctgtaaaca gacgggcagc agcgccgatt gatagcgcgc ccgcgcctgc ggcagatggg
     2341 cgcgtgagcc gttgaccagc acgtcgaatc cggcgtgcag ccagaggtcg atctcgacgc
     2401 cgacgccata atacagaccg ttagcgtgcc agcttaaggc cagtaggttc tgccccgcgc
     2461 gggtaaaaaa ctcctgctcg ctcagagcga tatggttttc gcttccggcg ctggcgtcgc
     2521 gcgtgatgta gcgatgcgcc accagtagct gagtttgttc ccgcaggcgg agttccgcca
     2581 gcaggctatc tttcccggag ccggacggcc ccattaacca aatcagtttt cccatcatca
     2641 gaacaccctt ttaccctgac gccagacgtg gtcgatatga atgtgattgc ccttgcgatg
     2701 cgccagcacc aagtccgcgc gtttgccctc gccaatcacc ccgcgatcct ggagattaag
     2761 cgcctgcgct ggatttttag tcaccagctt caccgcctgc ggcagcgtaa agcggttgct
     2821 ctcgtcatcg gcgacgcgaa atgccgcatc gagcaggctg gcggggtagt agtcggaaga
     2881 gaggatatcc agcaggccaa gctgcgccag ttcactggcc gccacgttgc cggagtgcga
     2941 gccgccgcgc acaatattcg gcgcgcccat cagcacgttc atgccatgct tgcgcgaggc
     3001 ttccgccgct tcgaacgtgg tgggaaattc ggcgatcacg ctgccaagct ggtgagattc
     3061 agcaacgtgg gcgtgggtgg cgtcatcgtg gctggcaagc gcaatttttc gcgcgcggca
     3121 cagggcggcg atggattcgc gattcggctg cgaccagcgt gcggcgagcg ccagttgctc
     3181 ttcttcgtac tgctgcatct gcgcatcagt gagggagtat ttgccctgat aatattcgcg
     3241 atacttctcg cgattggcga actggcgctg gcccggcgag tggtccatca gcgacaccag
     3301 cgtcaccggc tcgcgctgca ccagtttttc aaacagcggc agcgtggtgt gatgcggcag
     3361 ttcgcagcgc agatgcagac ggtgctcggc gcggttgacg ccgcgtttct gcgtctcttc
     3421 gatggcgttg atcatcttct ccagattcac cagccgatcg ccgccgtcgc gcacgtcgcc
     3481 aattgccacg gcatccagta cggtggtgat gccgctcgcc accatcagcg cgtcgtggct
     3541 gctcatcgcc gagtgggcag gccagtcaac tttcgggcgc ggggtgaaga atttatccag
     3601 attatcggta tgcagctcaa tcagccccgg cagcagccag ccgccttcgc cgtccatcgc
     3661 ctccggcagg cggctctggc tttcggcaaa ggcgcggatt tcgccgtcct gcacctccag
     3721 tgaaccgtgt accacttcgt tttccagcac caatttgacg ttattgataa tcatgaagag
     3781 gcttccattg ggtgcaggcg gtcggcaacg tcattacgta cggcttcgtc gtggaagatg
     3841 ccgacgatgg ccgcgccacg ggctttggct tcgtgaatca gctcgactac ggcggcgctg
     3901 tttttggcgt ccagcgaggc ggtaggttcg tcgagcagca gaatggggta gtcgacgata
     3961 aagccgcggg cgatgttgac gcgctgctgt tcgccgccgg agaaggtcga tggcgccaga
     4021 tgccacaggc gttccggcac gttcaggcgg gtgagcagac gcgcggcttt agcggcgcag
     4081 gcttcacgcg gtacgccggt atcgagtagc ggctgcatca ccacttccag cgccgaaata
     4141 cgcgggatga cgcgcagaaa ctggctcacc cagccaacgg tggttttgcg gatctccacc
     4201 actttgcgcg ctggcgcggt gaccagatcg acccattcgt caccgtgttt gatctggatt
     4261 tgcccttcgt cgggcagata gttggcgtac agcgagcgta gcagggttga ttttccgctg
     4321 ccggaatggc cgtggagcac cacgcattcg cccgcgttga cgatgagcga ggcgcggttg
     4381 aggacgggca ggcgcacgcc gttttgctgg tgcaggatga aggttttact gacgttttgt
     4441 acgttaatca tttttcgcct cgtggttcgg gtgtcggatg cgacgctgac gcgtcttatc
     4501 cgacctacgg ggagcgcatt tgtaggccgg ataaggcgtt tacgccgcat ccggcaccgg
     4561 ctcaattctg caaaaccgat gacaccagca gctgtgtata cggatgatgc gggtcgtcga
     4621 gcacgcggtc ggttaaccca ctctccacca cttgcccctg cttcatcacc agcaaacggt
     4681 ccgccagcag gcgggcgacg cctaaatcgt gggtgacaat cactaccgcg aggttcagct
     4741 ccaccaccag tccgcgcagc aggtcgagca ggcgggcctg caccgacaca tccagcccgc
     4801 cggtcggttc atccataaac accagcttcg gatgcgtcac caggttgcgg gcaatctgca
     4861 aacgctgctg cataccgccg gaaaaggtgg tcggcaggtc gtcgatacgg ttggcgggaa
     4921 tctccacctc ttccagccac ttctgcgcgg tagcgcgaat atcgccgtaa tgtcgcgccc
     4981 cggtcgccat cagccgctcg ccgatattgc cgcctgccga cacctggcgg cgcaggccgt
     5041 cgagcggatg ctgatgcacc acgccccatt cggtgcgcag caggcgacgg cggtcggcct
     5101 cgctcattgc atagagggaa cggttttcat agtgaatctc cccctgctgc ggcgtcagac
     5161 gcgcggagat cgacttcagc agcgtggtct tcccggagcc ggattccccg acaatgccca
     5221 gcacttcccc cggccataaa tcaaaagaga catcgctaaa gcctttgccc ggcgcgtaaa
     5281 ggtgggtcag gttattgacc gaaagtaacg gttgattcat tggcttttcg cctcgctctg
     5341 ttggcggcaa taatcggtat cggagcagac aaacatgcgg tttccggcgt catccagcac
     5401 cacttcatca agatagctgt gggtcgatcc gcagatggcg catggctcat cccactgctg
     5461 aacggtgaac gggtgatcgt cgaaatcgag actttccacg cgggtaaacg gcggcaccgc
     5521 atagatgcgc ttctcgcgcc ctgcgccgaa cagctgcagg gcgggcatca tgtccatttt
     5581 cgggttatcg aatttcggga tcggcgacgg gtccatcacg tagcgcccgt tcaccttcac
     5641 cggataagca taggtggtgg cgatatgacc gaagcgggcg atatcttcat acagtttcac
     5701 ctgcatcacg ccgtactcct ccagcgcgtg catagtgcgg gtctccgttt cgcgcggctc
     5761 gataaagcgc agcggttccg ggattggcac ctggaagata atgatctgat cttcggtcag
     5821 cggcgtttcg gggatgcggt gacgcgtctg gataagcgtc gcatcgtccg tacattcagt
     5881 ggtgtttacc ccggtcacgc gcttaaagaa gttgcgaatc gacacggcgt tggtggtgtc
     5941 atccgcaccc tggtcaatca ccttcaacac gtcgctttcg ccaatcacgc tggcggtgag
     6001 ctggatacca ccagttcccc agccgtacgg catcggcatc tcgcgcccgc caaacggtac
     6061 ctgataaccg gggatcgcca ccgcttttaa gatggcgcgg cggatcatgc gtttggtctg
     6121 ctcgtcgagg taggcaaagt tgtagccgct cagattagcc atggttctgc tcctgttgca
     6181 gacgtttgag tagctccagt tcggcctgga aatcgacgta gtgcgggagt ttgaggtgcg
     6241 agacaaagcc tgcggcttcg acgttgtcgg catgcgccag cacgaactct tcatcctgcg
     6301 ccgggcctgt cgcgtgctcg ccgtattccg gggcttgcaa cgcacggtca accagcgcca
     6361 ttgccatcgc tttgcgctcg ctcatgccga ataccagccc gtagccgcgc gtgaagtgcg
     6421 gcggctcatc cggcgggtcg ataaaaccgt tgaccatttc acactcggtc atcagcagtt
     6481 cgccgacgtt taccgcaaat cccagctctt ccggcacaat cgacacgtcg atgtaaccac
     6541 tgcggatctc gcccgcgaac gggtggttgc gcccgtaacc gcgctgggtg gagtaagcca
     6601 gcgccagcaa atagccttcg tcgccgcgca ttaactgctg caaacgggaa gaacgtgaac
     6661 aggggtaaac cggcggcgtg cgggtgatgt catccggctg tgctccgcta tcctcttcaa
     6721 acttcgccag cccctgacgc gccagcaggc tgaaaacgtg cggcgacggc tgttgttcgc
     6781 tgtcggcggt ggtcagcgtc ggcgcttcgc cgtttgccag cagagtgaaa tcgagcaggc
     6841 gatgggtgta gtcgtaggtt gggccaagca gctggccgcc gggaatatct ttataaaccg
     6901 ctgaaatacg ccgttcgaga cgcatctccg tggtgtcgag cggctcgctt accgccagtt
     6961 tcgtcagcgt ggtgcggtag gcgcgtagca ggaaaatcgc ttcgacgtta tcgccgctgg
     7021 cctgtttcag cgccagcgcc gccagttcgc ggtcggcaat gccgccttct gtcatcacgc
     7081 gatccaccgc gaggttaagc tgctgttcaa tctgggcgac gctcagttcg ggcaaatcgg
     7141 tatcgccccg gcgtcggctc tcttgcaggg cgtgggcggc gtcgatcgcc ttttcgcccc
     7201 ctttcacggc aacgtacatc agcacacctc cacatgagtg gttcgcggga tagccagcag
     7261 gcgctcgccg caggtcagga ttaggtcgat gccgagcggg aacgggtgcg ggcgctcggt
     7321 gagttcgtgc agaatgcact ctggcagctg cggagcgatc attcgttctt cggcaatgcc
     7381 cgcgccggtg agacgcaaca tgcgtccgcc gctcaggctg gcgacctgta aaatcagcgt
     7441 cgcgcccgct tccggcgcaa cggcggtgtc ggtggaaagg gcgttgagct gttcgctgga
     7501 aatcgcctca tccgtcaccg cgaaggtcgc ctgttccggc tggctgacca gcggcgcgtt
     7561 ggtatgaaaa cgcaggctct gattgacgat atcgttactt aatggcgtgg caagccacac
     7621 cggcgtgtcg ttatcggcca gcgtcagcag cacgctggtg gtggcgatat tcagcggctg
     7681 ccagccgcgt ttgagctgat acagggcgac aatcacgccc ggctcgctca tggcctttaa
     7741 caggcgacga aaactgtgct gggcatcctg cacgggaagc ataaaagcgg tttccagggt
     7801 catgcgttgt ctccgcgaac catcgtaaag aagtcgaccc ggctggcgtt cacttcggcc
     7861 tggcgtgcgg caatgcgtgc catacggtca gcgtccagcg gggcaataag ggtttctgat
     7921 aagttttgaa agtggcgaga ttgctgcatc agcgcatcaa tcagcgcgca gcgttcggcg
     7981 tgctgtttat cacgccccag cacccagctg tagccgaggg tgccgtcagt caggcgtacg
     8041 gcggcacggg tcagcgtggc gtcgccggca aaaaagcgtt cgccggtacc gcccattcgc
     8101 gcctgaatct gcaccaggcc agtttcggca gtgcggatca cttcatagtc ggcggtgatg
     8161 ttcagcgcgt tcaggcgggc tgccagttca gccggttggc tgtgcgccag tacggacatc
     8221 cagtgctggc gggtcgcggt atctgcgtgc attcagtgct ccatagtgaa ttcaatcatg
     8281 tcggcgcgcg tcaggctgac ggagtactcc gccgggctgc tttcaccgtc acggtggtta
     8341 agggtgcgca cgcacagcag cggcgacata ttcgggattt caaggcgctg gcactctttg
     8401 gcctgggcgc ggcgggcgct gatccgcgtc tggctgcggc gcagcgcaat tccggtttgc
     8461 tcgcgcagaa aatcgtgcag cgaaccgctg tcgaaacgtt gcagtgtcgg ccagagggtg
     8521 aggtccgcaa agtagtggtc gattaaacag agcgcgacgc cgttgacgcg acgcagggtg
     8581 cgcaaatgga taacgttctc accctcggta atccccagcg cgtcagcaac gtgaccggac
     8641 gcggggcgca ataccgacag cagtttttcg ctggtgggat ggctgccctg gtccagcagg
     8701 ttctggctaa aacgcgcctg ggcgttgagc gggtaatcga acgggcgcat cagcaccagc
     8761 acgccgatgc cctgacggcg ctgtacccag cctttttcca ccagttggtc gatggcgcgg
     8821 cgcagggtgt ggcggtttac ctcaaagcgc gccgccagtt gctgctcggc gggaagatag
     8881 tcgccgcagc gatagtgttg acgaagctcc tgctcaagtt ttgcggctat ctcctgatag
     8941 cgtgttgggt agctggtcgg atgtgtagac aagtgcatag atatcaatgc ctcacttatc
     9001 agataaagtg cttacgcaac cgttgagaga ggaaatccag caggctgacc gtgacgatga
     9061 taagcaccat cagggcgcag gtttgttgga actggaaacc gcgaatcgct tcccacaggg
     9121 tgacgccgat cccgcctgca ccgaccatgc cgacgacggt cgccgagcgg acgttggatt
     9181 cgaagcgata gagggagtag gagatcagca gcggcattac ctgtggcagc acgccgtaaa
     9241 ggatctcttc cagcttgttg gcgccggtgg cgcgaatgcc ttccaccggg ccgggttcaa
     9301 tcgcttccac cgcttcggaa agcagtttcg agagcacgcc ggtggtgtga ataaatagcg
     9361 ccagcacgcc agcgaacggt ccgagaccga cggcgaccac gaacagcatg gcgaagacca
     9421 tttcgttaat ggcgcggcag gcgtccatca ggcggcgaac gggctggtaa acccaccacg
     9481 gcaccaggtt ttcggcgctc atcaggccga aggggatgga gagaaccact gccagcgcgg
     9541 tgccccagac ggcgatttgc agcgtgacgg ccatttcggt gaggtaatcc tgccactggc
     9601 tgaaatcggg cgggaagaag tcggcggcga aggtcgccat gttgccgccg tctttgatca
     9661 gcgtaagcgg ggccatttcc gcgccctgcc acgagacgac caacacggcg agaacaatgg
     9721 cccagctcag aagcgagaac cagctgcgct tgggtggggc gatggtgatg gtttgcatgt
     9781 ttggctccgt ttaatgtttt ctccctctcc ctgtgggaga gggccggggt gagggcatca
     9841 gcgtgcgcat ttccccctca ccctaaccct ctccctcaag ggagagggga ccgatcgagc
     9901 acattactgc accgctttac tcaccgaact catcgcgctt aacgcgttgt tcaggcggtc
     9961 caggtcatcc agctgcgcct gaatctcggt ggttttcgcc agcttgtcct gctcattcag
    10021 tccgttattg ctttttacgc cctgaatctc tttaaacagt gcgagctggc gaatcggcac
    10081 cagttgcagg tcgctggagg cgcgaaacgg tgcccagccc aggcgttcca gcaccgcttt
    10141 ctcttccggc gtttttccgt agttcataaa gaagtcgtag atcttgtctt tggtggtttc
    10201 ggagagattc ttgcgccaga cgatcggatc gcctgggatc agcggcgact tccagatcac
    10261 cttcagttct ttcagcttct ctggcgcgga ggttttcagc ttgtcgaggt tttcggtgtt
    10321 gttggtggca acatccacct gcttgttggc gacggccagc gcgttggttt catgcccggc
    10381 gttgacggtg cgcttaaagt cgctggcgga gatattgttt ttggcaaaga cgtagtaacc
    10441 ggggacgagg aagccagagg tggagttagg atcgccattg ccgaaggtga gatctttccg
    10501 cttcgccagc agatcgttca ggttgttgat cggactatct ttgttgacga tcaacacgct
    10561 ccagtaacct ggcgatccat ccgccgcgac cgtctgggcg aagacctggc cgttggcgcg
    10621 atccaccgct tccattgccg acaggttgcc gtaccaggcg atatccactt tattgaagcg
    10681 cattccctgg ataatgcctg cgtagtctgg ggcaaagaag gcgttcacct tcacgcccag
    10741 cttcttctcc atatcctgta agaatggcgt ccattgcggt ttcaggtttt gctgtgattc
    10801 cgttgaaata atgccgaaat tcagcgcctt ttcctgctct tcggcgtgtg ccgggcttaa
    10861 cagggtgctg aggctgaaca tgctggtgaa ggccagcgag gcaattatct tagcgttcat
    10921 tcgttttcct caatgatggg gatgtcaggc agctttcgcg ttctcttcga tgcgattaat
    10981 gctgcggtag agatggtcaa aacgttcgtt atcaaactgt tggctgctgc cgtcgtagaa
    11041 aacgtgcccc tggcgcaggg cgacgatgcg ttcgcagtag cgcagggcgt aatccacctg
    11101 atgcagcgtg acgaccacgg tgatgccgtc gttctgattg atgtcgcgca gggtgtccat
    11161 cacgatgcgg gcggattccg ggtccagcga ggcgatgggt tcatcggcca gaatcacctt
    11221 cgcctgctgc atcagcgcgc gggcaatcgc cacacgctgc tgctgtccgc cggagagggt
    11281 ggaaacgcgt tgatgggcaa aatgcaccat gccaacgcgg gtcagcgcct gtaacgcgcg
    11341 ttgtttctgc tcgcgggtaa accagctaaa acaggtgcgc cagaacggcg tgctgccgag
    11401 cgcgccaatc agcacgttct ccagtacgct caggcggttc accaggttga attgttggaa
    11461 gatgtagccg gtgttggcgc ggcttttgcg gatatcgcgc gccagacggc cttcgcgctg
    11521 gactgtgcgg cccagcagct cgatatggct gccggcggat ttatcgccgg taatcaaacc
    11581 gcttaagtga cgtaaaaggg tggatttgcc ggaacccgac ggcccaagca gagccaccat
    11641 ttcaccgtga tgaatgttca gagcaaccgc atgcagcgcc tgatgctgat tgaaggtttt
    11701 ggcgagcttc tcgacacgga taatcgtttg catgatgcag cctccctaaa aaagtggctc
    11761 catcgtggcg gattattgtg acgatttggt taattaaaag tgactaacag atgaagagtt
    11821 aacgggaatt cgatgacagt gcgggagggc gggcctcccg gcgggttatt gcgttggttg
    11881 ttgtttgacg acattaatca tccacggtac gccaaattta tcggtgactt tgccaaagcc
    11941 atgcgcctag aaagtttcct gccaggccat ttcgattttt ccgttagcgg caagattgtc
    12001 aaaccagcgt tttccttctt cgacctgttg cgaatcgagc accagcgtaa agccggagta
    12061 gctggctttt cctgacggca tggcatcgct catcatgatg tcgcttccgg caatgcgcac
    12121 attggcgtgg gcgatggcgg tatcgggaaa ttgcattccg gaagggcagt tctcggcgct
    12181 gtcctgcgct gattttggca tttcgccgaa gctgatttta tagagcagtt cagcgcccaa
    12241 cgtacgttga taataagcaa ttgcgtcggc acagttaccg gcaaaagaga ggtagggact
    12301 taacggcatg atggtgacct cagttaagag aagccaatta agtgtagttc gaaatttata
    12361 cagatgagag gctgcctgat aagacgcgca agcgtc
//
LOCUS       DAZOYL010000108        12071 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZOYL010000108 DAZOYL010000000
VERSION     DAZOYL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12071
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.108"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            311..1081
                     /locus_tag="ACHUOY_002166"
     CDS             311..1081
                     /locus_tag="ACHUOY_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR0682939.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1072..1545
                     /locus_tag="ACHUOY_002167"
     CDS             1072..1545
                     /locus_tag="ACHUOY_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR0682940.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1653..2192
                     /gene="dnaT"
                     /locus_tag="ACHUOY_002168"
     CDS             1653..2192
                     /gene="dnaT"
                     /locus_tag="ACHUOY_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR0682941.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2195..2932
                     /gene="dnaC"
                     /locus_tag="ACHUOY_002169"
     CDS             2195..2932
                     /gene="dnaC"
                     /locus_tag="ACHUOY_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR0682942.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2981..3475
                     /gene="yjjA"
                     /locus_tag="ACHUOY_002170"
     CDS             2981..3475
                     /gene="yjjA"
                     /locus_tag="ACHUOY_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR0682943.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3729..6020
                     /gene="opgB"
                     /locus_tag="ACHUOY_002171"
     CDS             3729..6020
                     /gene="opgB"
                     /locus_tag="ACHUOY_002171"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR0682944.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6159..7181)
                     /gene="lgoD"
                     /locus_tag="ACHUOY_002172"
     CDS             complement(6159..7181)
                     /gene="lgoD"
                     /locus_tag="ACHUOY_002172"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR0682945.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7320..8234
                     /locus_tag="ACHUOY_002173"
     CDS             7320..8234
                     /locus_tag="ACHUOY_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR0682946.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8449..9800
                     /locus_tag="ACHUOY_002174"
                     /pseudo
     CDS             8449..9800
                     /locus_tag="ACHUOY_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9849..11504)
                     /gene="tsr"
                     /locus_tag="ACHUOY_002175"
                     /pseudo
     CDS             complement(9849..11504)
                     /gene="tsr"
                     /locus_tag="ACHUOY_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(11623..12069)
                     /gene="hpaR"
                     /locus_tag="ACHUOY_002176"
     CDS             complement(11623..12069)
                     /gene="hpaR"
                     /locus_tag="ACHUOY_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR0682947.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         2934 a         3190 c         2947 g         3000 t
ORIGIN      
        1 aatcacacaa ccaatattta ttcaatgaaa tctgatggtt ttaacttgtt aacattaaaa
       61 aacgtgcccg gttatctatt atcctgattc gtaaaggcca aacaataaat tcattatagt
      121 gataatattc atgtgatagt ttgtttgcgg cgagagataa ttcgcttttt atcaccgagc
      181 ttgtcccgat aaccaggatt aatcagcgca attttgatct taacctaccg caaaacaacc
      241 gtgcttcatg atacaaatgg attctcatta tctgaacaat acgcagcacg tctatgacaa
      301 agggcgagtt atgcaaactg agcaacagcg agccgtaaca cggctttgta tccagtgtgg
      361 attatttctt ttgcaacatg gtgcggaaag cgcgttggtt gatgagcttt cctcacgact
      421 gggtcgggcg ttggggatgg acagcgtcga aagttctatc tcttcgaacg ccatagtgct
      481 gacaaccatt aaagatgggc aatgcctgac atcgacacgt aaaaatcacg atcgcggcat
      541 taatatgcat gtggtgactg aagtccagca cattgtgatt ctggcggagc accatctgct
      601 ggattacaaa ggcgtagaga aacgatttag ccaaattcag ccattacgtt acccaagatg
      661 gctggtagcc ttaatggttg gcctttcttg cgcctgtttc tgcaaactca ataaaggtgg
      721 ctgggatggt gccgtcatca ccttctttgc cagtacggcc gcgatgtata tccgccagct
      781 gctggcacaa cgtcatcttc atccgcagat caacttttgc ctcaccgctt tcgccgccac
      841 caccatttcc ggattgcttt tgcaacttcc cactttcagc aatacaccca ctatcgcgat
      901 ggccgccagc gttctgctgc tggtgccggg atttccgttg attaatgccg tcgccgatat
      961 gtttaaaggc cacatcaata ccggactggc acgctgggcg atcgccagtc tgctgacact
     1021 ggctacctgc gtcggcgtgg tgatggcact gacgatttgg gggctacgcg gatgggtgtg
     1081 atcgagtttc tgtttgcatt ggcgcaggat atgatcctcg ccgccattcc agcggtcggc
     1141 tttgcgatgg tgttcaacgt tcccgttcgg gcattacgct ggtgtgcgct gcttggcgcg
     1201 attggtcatg gttcacgaat gatcttaatg accagcgggc tgaatattga gtggtcaacc
     1261 tttatggcct ccatgctggt cggcaccatt ggtattcaat ggtcgcgctg gtatctggcg
     1321 catccgaaag tgtttaccgt ggcggccgtt atccctatgt tcccgggcat atcggcttat
     1381 accgcaatga tttcggcggt aaaaatcagc cagttaggtt acagcgaacc gttgatgatt
     1441 accctgttaa ccaactttct tacagcttca tcgattgttg gtgcgttatc catcggtctt
     1501 tccattcctg gattatggtt gtaccgcaag cgccctcgcg tataaaattg cctcgcttaa
     1561 agcacacgga tgagagacag cctcctctcc tccgtgtgtt actataaaag ttatctccct
     1621 tctcgttcat cgttccatat ttgagaaaca gtatgtcttc cagagttttg accccggacg
     1681 tcgttggtat tgacgccctg gtacacgatc accaaaccgt tctggcaaaa gctgaaggcg
     1741 gtgtggttgc cgtatttgct aacaatgccc cggcgtttta tgccgtcacg cctgcacgcc
     1801 tggctgaact gctggcgctg gaagaaaagc tggcgcgtcc gggaagcgat gtcgctctgg
     1861 acgatcaact ctatcaggaa ccgcaagccg ctcccgttgc tgtacccatg gggaaattcg
     1921 ccatgtatcc ggactggcaa cccgatgccg attttatccg cctggcggcg ctatggggtg
     1981 tggcactaag agagccggtg accaccgaag aactggcctc attcattgcc tactggcagg
     2041 cggaaggtaa agtctttcac catgtgcagt ggcaacaaaa actggcgcgc agcctgcaaa
     2101 tcggtcgtgc cagcaacggc ggactgccga aacgagatgt gaatacggtc agcgaacctg
     2161 acagtcaaat tccaccagga ttcagagggt aacgatgaaa aacgttggcg acctgatgca
     2221 acgcctgcaa aaaatgatgc ctgcccatat caaacctgca ttcaaaacgg gtgaagaact
     2281 actggcctgg cagaaagaac aaggagcgat ccgctccgcc gctctcgaac gtgaaaatcg
     2341 ggcgatgaaa atgcagcgca cctttaaccg ctccggtatt cggccactgc atcagaactg
     2401 ctcctttgag aactatcgcg ttgagtgtga agggcagatg aatgcgttaa gcaaagcgcg
     2461 ccagtacgtc gaagagtttg acggcaacat cgccagcttc atcttttctg gtaagccagg
     2521 aaccggcaaa aaccatctgg cggcggcaat ctgcaacgag ctgctgctac gcggtaaatc
     2581 cgtattgatc atcaccgtgg ccgatattat gtcggcgatg aaagatacct tcaggaatag
     2641 cggtaccagt gaagaacaac tgcttaacga tctgagcaac gttgatctgc tggtgatcga
     2701 tgagatcggc gtgcagaccg aatcgaaata cgaaaaagtg atcatcaacc agatcgtcga
     2761 tcgccgctct tcttccaaac gcccaaccgg gatgctgacc aacagtaata tggaagagat
     2821 gaccaagttg ctgggcgaac gcgtgatgga ccgtatgcgc ctgggtaaca gtttgtgggt
     2881 gatcttcaac tgggatagct accgtagccg ggtaacaggt aaagagtatt aatccccgtt
     2941 tctggcggta tactgcgaca caattccacc ttgagtaatg atgatgaaaa ctgttaaaca
     3001 tcttctgtgc tgtgccattg ccgccagcgc attaatttcc accggggtgc atgctgcgtc
     3061 ctggaaagat gcgctctcca gcgccgccag cgaacttggc aaccaaaaca gcacgacaca
     3121 ggaaggcggt tggtcgctcg cgtcattaac taacttgctt agcagcggga accaggcctt
     3181 aagcgcagat aacatgaaca acgccgcagg cattctgcaa tactgcgcga agcaaaagct
     3241 ggcgtcggta accgatgccg aaaacatcaa gaaccaggtg ctggaaaagc tgggcctgaa
     3301 cagtgaagag caaaaagaag acaccaacta tctggatggc attcagggtt tgctgaaaac
     3361 caaagatggt cagcaactca atctggataa catcggaacg actccgctgg cagaaaaggt
     3421 gaaaaccaaa gcctgcgatc tggtgttaaa gcaggggctg aacttcattt cctgaaccgc
     3481 gaaacgccgc gttttagctg ttacccggct gacgcggctc tctcctgccc ttttccctgc
     3541 tcctggacgg ttttccccct gttacacggg aagcgaccgc gatcaaaaaa gtctgtttat
     3601 aaactaattc ctaacaacat cgcatttttt tgacactaga attgcagcaa tatggcgacg
     3661 ccattacatt gtgcttctca aaaagcttaa acgttggcta aacggcccgg tttagtgagg
     3721 atcgcccgtt gtcagaacta ctctctttcg ccctttttct cgcctctgtg ctgatttacg
     3781 catggaaagc gggacgtaac acctggtggt ttgcagccac attaacggtg ctggggctat
     3841 ttgtcgtttt aaatatcacc ctgtttgcca gcgactattt tactggcgat ggtattaacg
     3901 acgcggttct ctatacctta accaacagcc tgaccggtgc tggcgtcagc aaatacatcc
     3961 tgccgggtat cggcattgtg ctggggctga cagcggtgtt cggtgcgctg ggctggatcc
     4021 tgcgccgtcg tcgccatcat ccgcaccatt ttggttacag cctgctggcg ctcttactgg
     4081 cgctgggttc ggtggacgcc agcccggcat ttcgtcagat aacggaactg gtgaaatccc
     4141 agtcacgcga tggcgacccg gactttgcgg cttattataa agagccgtcg aaaactatcc
     4201 ctgacccgaa actcaacctg gtttatatct acggcgaaag tctcgagcgg acctattttg
     4261 ataacgaagc tttcccggat ctcacgcctg aactgggcgc gttgaaaaat gaaggcctgg
     4321 atttcagcca cacgcagcag ctgccaggaa cggattacac gattgcgggc atggtggctt
     4381 ctcagtgcgg cataccgctg tttgccccct ttgaaggcaa cgcctccgcc tctgtctcca
     4441 gcttcttccc gcagaacatc tgtctgggcg atatcctgaa aaactcgggt tatcagaact
     4501 atttcgtgca gggcgcgaat ctgcgttttg ccggtaaaga tgtgttcctg aagtcgcatg
     4561 gcttcgacca cttatacggc tcagaagagc tgaaaagcgt ggtggccgac ccgcactatc
     4621 gcaacgactg gggattctac gacgataccg ttctcgatga agcgtggaaa aagtttgaag
     4681 agctttcccg ctcaggtcag cgattctcac tgtttaccct gacagtcgat acccatcacc
     4741 cggatggttt tatctcgcgt acctgtaacc gcaaaaaata tgattttgac ggtaagccga
     4801 accagtcatt cagcgcggta agttgcagcc aggagaatat cgcggcgttt atcaacaaaa
     4861 tcaaagcgtc accgtggttt aaagataccg tcatcgtcgt ctcttctgac catttagcga
     4921 tgaacaacac ggcgtggaaa tacctcaata agcaggatcg caataacctg ttttttgtca
     4981 ttcgtggcga caagccgcag caagagacgc tggcagtgaa gcgtaacacg atggataacg
     5041 gcgcgacggt gctggacatt ctcggtggcg ataactatct cggacttggt cgtagcagtt
     5101 tatccgggca gtcgatgtcg gaaatcttcc tcaatatcaa agagaaaaca ttggcgtgga
     5161 agccggatat catccgcctg tggaaattcc ctaaagagat gaaagagttc accatcgacc
     5221 agcagaaaaa catgattgcc ttctcgggta gccatttccg tttgccgctg ctgttgcggg
     5281 tttcagacaa acgcgtggaa ccgctgccgg aaagcgaata ctcagcaccg ctgcgtttcc
     5341 agctggccga tttcgctcca cgcgacaatt tcgtctgggt tgaccgttgc tataagatgg
     5401 cacaactctg ggctccggaa ctggcactct ccaccgactg gtgtgtctcg caagggcagc
     5461 ttggcggtca gcaaattgtt cagcatgttg acaaaacaac atggcagggc aaaacggcat
     5521 ttaaagatac ggtcatcgac atggcgcgtt ataaaggcaa tgtcgatacg ctgaaaattg
     5581 ttgataacga tattcgctac aaagccgaca gtttcatctt caacgtcgcc ggtgcgccag
     5641 aagaggtgaa acagtttagt gggatttccc gtccagagtc gtggggccgc tggtccaacg
     5701 cgcagctggg cgatgaagta aaaatcgagt acaagcatcc gctgccaaag aaatttgacc
     5761 tggtgattac cgccaaagca tacggcaata acgccagccg tcctattccg gtacgcgtag
     5821 gcaatgaaga acaaaccctt gtgctgggca atgaagtgac caccaccaca ctgcatttcg
     5881 ataacccaac cgatgccgac acactggtaa ttgtgccgcc ggaacctgtc tcaaccaacg
     5941 aagggaatat cctcggacac tcgccgcgta agctcgggat cggcatggtg gaaattaaag
     6001 tggtggaacg tgaaggataa ttcctgactg atgtgtaggt cgcataaggt gcgacctaca
     6061 caccttctgc ggcctacaaa acatcattga gaaacagaaa aaaacagcgc ccaacacttc
     6121 cccggacgct gcatggcaac tttaataatc tcgccttatc agaaagtaat tacaccctta
     6181 atcaactcac gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt ggcgaacgga
     6241 tagcgatggg ttaacatcat gtcagcagtg atttttcctt ccgccatcag tcgaccgact
     6301 ttagcaaaat cttccggcgt ggcgttgcgg ctacccatca tcgtcgtttc ttttttatgg
     6361 aattccggat cggagaactg caactcacct ttaaacaggc cgacaaatac caccgtgccg
     6421 ccgtgacgaa tcaaattcac ggtgttattc atcgcatgtt gattacctgt cgcgtcgatc
     6481 actttctgcg ccagcgaacc accaaactgc gcccgtagct gcgcgtcaaa atcttcggct
     6541 gacgggtcca gtaaaggtaa ttccagacgc gttgccacat gttcacggcg cgccggactg
     6601 gtatccgcca ccaccacctg tgcgccatcg gctttagcga ttgccgccgc gcccagaccg
     6661 attggccccg ccccgaccac cagcacctgc tcgccgggag caatggctgc gcgacgcacc
     6721 gcatgagcgc taatagcgaa aggttcaatc aatgctgccg cctgcgggtc aataccgtct
     6781 gcgggcaaaa tgttcgccac cggcacgctc aaatactcac taaaaccgcc atcctgatgc
     6841 acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc cgcttttaca cgccgggcat
     6901 tgctgacagg caacataagg gatcacagca acttgctgac catttttaag atcagcaata
     6961 tttttaccca gcccaacaat ctccccacat atttcatggc ctaaaacacg tggataacta
     7021 aaaaatggtt gatttccacc ccaggcatga atatcggtac cgcaaatccc gacagactta
     7081 atttttatta atgcttcatt gtcacccgga ataggtatct cgcgttgttt ccagactaat
     7141 tctttcggct gctggcaaat taaaacattc atcgtagaca tgattcggct ctccattttt
     7201 tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat ctgttttgtg
     7261 aaaacacacg cataaaaatg ttttaaatcg ggttttaatg gactcacaag gaggaaaata
     7321 tgagtcgttc acaaaattta cgccacaatg tgattaacca ggtcattgat gatatggcgc
     7381 ggggtcatat cccttcccca cttccatcgc aaagtgcgct ggcggaaatg tacaacatca
     7441 gccgcaccac agtgcgtcat attctcagcc acttacgcga atgcggcgtc ttgacgcagg
     7501 ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga tgacggtttc gcctgtacta
     7561 ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg atgataaacc
     7621 agcgccagtt acgcccaggg gaaacgtttt ctgaactgca actggctcgg gccgcgggtg
     7681 tcagcccggt tgtagtaaga gaatatcttt taaaattcgg ccgttataat cttattcaca
     7741 gcgaaaagcg cggccagtgg agcatgaagc aattcgatca atcctacgcc gagcagctgt
     7801 ttgagctacg cgaaatgctc gaaacacatt cacttcagca ctttctcaac ctgcctgatc
     7861 atgatccccg ctggctgcag gcgaaaacca tgctcgaacg tcatcggcta ctgcgtgaca
     7921 acatcggcaa tagctttcgc atgttctcgc aacttgacag agattttcac tccttactgc
     7981 tttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca gtcatctttc
     8041 attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt gccgtcgatg
     8101 agcatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc acccttgcgc
     8161 tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat gagaacacgc
     8221 gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca cggttttatc
     8281 aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg actaaaaatg
     8341 tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc cgttaatgtc aaccttgcgt
     8401 aattcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt ggaaaaagaa
     8461 aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga tattcccgtg
     8521 ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac caccgccatg
     8581 ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct gtcggtagca
     8641 aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc tttgctctcc
     8701 gtgttttcac tcgcttacgg gattgcgcaa cttccttgcg gcccactatt ggatcgtaaa
     8761 ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca ggcaatgtct
     8821 ggcatggtgc acaactttac gcagttcgtg ttggtgcgta tcggtatggg gattggtgaa
     8881 gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat caaagagcgc
     8941 ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgccgt aagcccaccg
     9001 attctggcgg cgatgatgct ggtgatgggc tggcgcggga tgtttattac cattggtgta
     9061 ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga gcacgtagaa
     9121 ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg ccgagatccg
     9181 ctcagttttg ccgaatggcg cagcctgttc cgtaaccgta caatgtgggg aatgatgctc
     9241 ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc tggttacctg
     9301 caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat ccctttcctg
     9361 tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt caaaggggga
     9421 atggctccga ttaaaagccg taagatctgc attattgccg ggatgttctg ttctgccgcc
     9481 tttacgctgg tcgtaccgca agcgacaaca tccatgacag cggttctgct gattggcatg
     9541 gcactgttct gtattcaaca tcctgctggg gcttgatcca cgtcgcagtt gcttctcgca
     9601 tgactgcgtc ggtgggcagt atccagaact ttgccagctt catctgcgcc tcttttgcgc
     9661 cgatcattac tggttttatt gttgatacca cccactcatt ccgtctggca ctaatcatct
     9721 gcggttgcgt caccgcagcg ggggcactgg cgtacatctt cctggttcgt cagccgatca
     9781 acgacccacg taaagattaa tctccttatg cccgataaca ttttgcttat cgggcatttt
     9841 catggcgatt aaaatgtttc ccagttctcc tcgctatctg ccacggccat tttacgcggc
     9901 gcagctggcg tcacggtttt taccacagcc gatgtttcac gctgctgttg ctgaatccgg
     9961 aacactgcca ctgcttcggt caggcgactg gcctgctctt ccagcgcggc ggcggcagcg
    10021 gcagactctt ccaccagcgc ggcgttctgt tgagttaccc ggtccatctc agcaaccgct
    10081 aagccaacct gatcgatacc acggctctgc tcatcagaag cagaagcaat ttcgcccata
    10141 atgtccgtca cgcgggtcac ggcgctgaca atctccgcca ttgtttcccc ggcgctttcg
    10201 accagcgtag agccaacatc cactttcccc accgagtctt caatcaggct tttaatttca
    10261 cgagccgcct gggcgctgcg ctgggccaga ttacgcactt ctcccgcgac caccgcaaaa
    10321 ccgcgccctt gctcacccgc acgcgcagcc tcaaccgccg cgttcaaagc cagaatattg
    10381 gtctggaagg caatgccgtc aattacgctg ataatatcgg cgattttctg cgaactggtg
    10441 gagatatcgc gcatagtctg caccacgtta tctaccactt tgccgccgcg ttgcgccgtt
    10501 tcagaagcac ttaacgccag atggctggcc tggcgcgcat tttcggcgtt ctgcttcacc
    10561 gttgcggtca gttactccat gctggctgcc gtctcttcca gcgaagcggc ctgttgctcg
    10621 gtgcgcgaag agagatcgtt attgccggtg gcgatttcgc tggcaccgct atagatggca
    10681 ttggccccgt tgcgcacatc accgacggta cgcatcagct ctccctgcat atggcgcaaa
    10741 ctctctgcca gttgccccat ctcattagag ccatccacct caatcggttt caccagatcg
    10801 ccgcctgcaa tatgacgaat gctgtcaatc aggcgattca ttggcgctac cagcgaggct
    10861 ttaataccga accagacggc gaagatgacc gccagtacga cgatcatcac gcccaccaga
    10921 atccacatcg cctggctgta ggaggcattg ttatcgctga cggcgatatc atagagccga
    10981 tcgttttgct ccatgtaagc cacatactgc ttctcgaaac cgtcctgata tccctgggtc
    11041 ggctgatcaa agaactcgtt gattttgcct gcacctaaca gttggatcag ctccgccagc
    11101 gcattgtgat aaatatcgta attacgtttg atctctgccg ctgcggcggt gctctgacgc
    11161 gggtcacgcg gcaacgcttc gtaatccgcc cagttttttt ccgcctgttt cagcgaaata
    11221 ctggcactct gcatcagctc agcaacggtt gaaccgctac caatattgtt ctgatccatc
    11281 atgtagcgga tacccgcgcg gttgagggtg ttacgcgtct gcaacaacgc gacccagctg
    11341 ccattcagcg tggattgctg ctggcgaatg gtttgtaaaa cagtgaaatt ttctttgtca
    11401 ttctttaagg cattaaagaa cagaccgcct gatgtcagtt gtaaaaggcc aaaaacggcc
    11461 aaaaccagca gtaagctggt cacgatcttg atacgtttta acatggtttc tctttcctgt
    11521 ggaccgatgc aagattttcg gcctggaaag caaaaacttt atgaaaaggt gtaacccacc
    11581 atccatggaa aaaccaggcg ctggttatca gatactaaaa agttattcat tatcgcccgg
    11641 gatggcttct tgtcgggaat tgcccagagc aataaattcc tctaacagat gggttaactg
    11701 ttgcattttc tcggcagtaa actgagcttc aatctgccga taggcttctt caatctgtgt
    11761 ctgagcgcgg ttatacagcg cctgcccctc tttggttagc gagatataga gcttccgctg
    11821 atcattgatc ggctttaatc gcaacactaa accgtcgcgt tccatccgcg tgaggatccc
    11881 ggtcaggctt gggcgcaaaa tgcaggcgcg atacgccaga tcgtgaaaat ccattgatgg
    11941 gctttccgcc agaatacgca cgatgcgcca ctgttgctca gtcaaattat gccgtttaac
    12001 gattgggcga aaataactca tcgccgcttc tcgcgcctgg agcaacgcaa tggttagtga
    12061 gtcgtgcatt a
//
LOCUS       DAZOYL010000109        11807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZOYL010000109 DAZOYL010000000
VERSION     DAZOYL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.109"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..487
                     /locus_tag="ACHUOY_002177"
     CDS             <1..487
                     /locus_tag="ACHUOY_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0682948.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            539..1474
                     /gene="ttcA"
                     /locus_tag="ACHUOY_002178"
     CDS             539..1474
                     /gene="ttcA"
                     /locus_tag="ACHUOY_002178"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR0682949.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHUOY_002179"
     CDS             complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHUOY_002179"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR0682950.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHUOY_002180"
     CDS             complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHUOY_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR0682951.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHUOY_002181"
     CDS             complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHUOY_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR0682952.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHUOY_002182"
     CDS             complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHUOY_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR0682953.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHUOY_002183"
     CDS             4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHUOY_002183"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR0682954.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHUOY_002184"
     CDS             complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHUOY_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR0682955.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6838..7746)
                     /locus_tag="ACHUOY_002185"
     CDS             complement(6838..7746)
                     /locus_tag="ACHUOY_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0682956.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7922..9232
                     /gene="abgA"
                     /locus_tag="ACHUOY_002186"
     CDS             7922..9232
                     /gene="abgA"
                     /locus_tag="ACHUOY_002186"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR0682957.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9232..10677
                     /gene="abgB"
                     /locus_tag="ACHUOY_002187"
     CDS             9232..10677
                     /gene="abgB"
                     /locus_tag="ACHUOY_002187"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR0682958.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10714..>11807
                     /locus_tag="ACHUOY_002188"
     CDS             10714..>11807
                     /locus_tag="ACHUOY_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0682959.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3005 a         2999 c         2927 g         2876 t
ORIGIN      
        1 gaatgtcaga agaaacctga cgatacttga tatgttcggt cccccaaaaa caaatgccgg
       61 gatccggacg gtaacattac tgcagccggc tcttgaagca ctgaaggagc aatacaaact
      121 gaccgggcat catcgcaaaa gcgaaatcac tttttatcat cgggagtacg gcagaactga
      181 aaagcaaaaa ctgcattttg ttttcatgcc cagggtgtgt aacgaaaaac agaaacctta
      241 ttactcggta agcagtttgg gtgcaagatg gaatgcagca gtaaaacgtg ctggtattcg
      301 ccgccgtaat ccgtaccata cgcgacatac ttttgcctgc tggctgttga cggcaggagc
      361 gaacccggca tttatagcca gccagatggg gcatgaaact gcgcaaatgg tgtatgaaat
      421 ttacggtatg tggattgatg acatgaacga cgaacagata gccatgttga atgcgcggtt
      481 atcgtagttg caaagtttgc ccccaatttg ccccatttag taccagagaa ctgaaataat
      541 gcaagaaaat caacaaatta caaagaaaga acaatacaac ctgaacaaat tacaaaaacg
      601 tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg
      661 catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa
      721 tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca
      781 accgggcttc ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa
      841 gattgtcgaa gagaacactt acggtatcgt gaaagagaag attccagagg gcaaaaccac
      901 ttgctcactg tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg
      961 ggcgacgaag atcgcgctgg gtcaccatcg tgacgatatc ctgcagacgc tgttcttaaa
     1021 tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa
     1081 acatatcgtt attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga
     1141 tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg
     1201 tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac
     1261 gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga
     1321 tttcaaaggc atcacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg
     1381 cgaagagatc ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga
     1441 tgagttacgg ctgaatgtgg ttgaagtgaa ataaccagga tggcgcccga tgcgcaagcg
     1501 tatcgggcta ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatccc
     1561 gaataagatg tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac
     1621 ccggctcgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac
     1681 agcctgacgg accgcgacat agacatgcgc cggatgcacg gcgattttgc caatatctgc
     1741 gccatcaagc ccgatatctc ctgtcagtgc acctaataca tcacccgggc gcattttggc
     1801 ttttttccca ccatcgatac acaacgttgc catttctgct tccagcggca caatggaact
     1861 attagctggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa tgatattggc
     1921 ccgctgtgct tcttccggag cacagaaact gatcgccaga ccgctatttc ctgcacgagc
     1981 tgtacggccg atgcgatgta catgaacttc agggtcccac gccagctcaa agttcaccac
     2041 cagctcaagc gatttaatat ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg
     2101 ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct ccaaatcgcc
     2161 gtgtaatgac aatgcacttt gccctacttc attcagcgcg tcgcagacag cctggcaatc
     2221 ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta ataaccgttg
     2281 caacagagga attttgcctt tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa
     2341 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattac
     2401 ggcaatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     2461 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     2521 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     2581 acgacccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac gctgcatacc
     2641 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     2701 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     2761 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     2821 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     2881 ggcggcctgc accggcgtca tagttaaata acccaactca ttaaggttcg tgagttgggc
     2941 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     3001 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     3061 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     3121 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     3181 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     3241 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     3301 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     3361 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     3421 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     3481 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     3541 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     3601 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     3661 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     3721 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     3781 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     3841 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     3901 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     3961 gctggaatga tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg
     4021 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     4081 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
     4141 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgagacggct
     4201 gactcggggc cgcgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
     4261 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
     4321 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
     4381 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
     4441 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
     4501 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
     4561 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
     4621 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
     4681 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
     4741 ggatcgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
     4801 tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag
     4861 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
     4921 ttcagcgtaa agaagaggaa acagcattaa actgtcggct tgttttgcga aagctgacag
     4981 aagcagaacc ggtgattatt ttcgaaggta tcgaagcgtc ggcaacgctg ggtttaaaag
     5041 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
     5101 ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta
     5161 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
     5221 gggaaataaa tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
     5281 ccggtggtca taaacctctg aattttattc ataagctggc ggatggttcg actcgtcatg
     5341 tgcagaccta cgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtac
     5401 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgcc caccatgacg
     5461 cgatgaccgg attactgaat cggcgacagt tttatcacat taccgagcca ggccaaatgc
     5521 agcatctcgc catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac
     5581 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca
     5641 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
     5701 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
     5761 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
     5821 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
     5881 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
     5941 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
     6001 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgca gtgcaatatg
     6061 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
     6121 gcgatttatc atcccgtcct ttgccatgaa taatcagcac gttacgcaaa ccatcagcca
     6181 tggcttgttg aatgaaactg aacatcattt tgcggcactc ttccaccggc tggcgcaaaa
     6241 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt
     6301 gcaacccttc ccgccgaaac tccagcggct gacttagtgg gatgatgtcg agaaatccgg
     6361 tggtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
     6421 ttggatgcca gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
     6481 gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat
     6541 gataaccaga ccgggtcggt ccaacaacgt attacccaaa attccagtaa taagttccaa
     6601 atattgccga tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa
     6661 tgtaacttat attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac
     6721 cgttataaca cagtcaccgg cgcagaggag acaatgccgg atttaagacg cggatgcact
     6781 gctgtgtgta ctgtagagtc tggcggatgt cgacagactc tattttttta tgcagtttta
     6841 actttgcaga tagccgcatt ctcgccggaa ttgcgtgatt aatgacgcgg tcagtggtgt
     6901 ctggcgacta tcacgccgct gaatcaaata ataggccgct ttcggtaaaa tttcgctaac
     6961 cggcaacatc accagcccct gtccgtgcaa gggatcgcag cccatttctt caggcagtat
     7021 gctgagaaaa tcgctttttg ccaccagact gatacaggct gagaacgtct cgcagactac
     7081 accgacctgt ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt tgtagtaact
     7141 gccgtgtggc gtcggcattg tccagctgta atccagtaac tgtttgatcg aacgggcacc
     7201 aatggcgggg tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta atttctcaaa
     7261 agtaaattcg tggtcgtacg gtccctgata ataggtattg atggtgaaat ccaattctcc
     7321 ctgacgcaat tcattaatca tcgacaccag ttgcccttcc ataatgcgta cttttacctg
     7381 cggatgctgc tgatgaaaac gagatatgac agctggcatc agactgcggg aaatactggc
     7441 ccccatgccg atattaatct gccctgccag ttgcccttgt cgttggcgaa tatcctcttg
     7501 ggctgcgcgc agctcttcaa gaataagact ggcgtgctga taaaaacttt caccggcatc
     7561 agttaacgtc acgcctttac tacggcgaaa aaagagttgc gccgctaatc cttcttccag
     7621 ctcctgaata gatttactca gtgccggttg cgacatattc aacattcggc tcgctccgcg
     7681 aatgctgccc tgacgagcca cttcaacaaa agcccgaatt tgatgaattt ttacctgaaa
     7741 agccatgacg ccaccgataa ccgttattta tcagaccaaa gaaactggca tctactttaa
     7801 tgcagatgat tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa tcagttagtg
     7861 ataagtaaaa actatcgcta cgtgaaccgg gtcacacttt ttactgatga cgggaaaggt
     7921 tatggagtct ttgaatcaat ttgttaattc gcttgcccca aaattatcgc actggcgacg
     7981 tgattttcat cactatgcag agtctggctg ggtggaattc cgcactgcca cccttgttgc
     8041 ggaagaattg cagcagctcg gctattcact ggcgctgggc cgcgaagtag ttaatgaaag
     8101 tagccggatg ggactacctg atgaactcac tctacaacgc gaattcgagc gcgctcgtca
     8161 acagggtgcg ctagcacaat ggattgcggt ttttgaaggt ggtttcactg gtatcgtcgc
     8221 caccctggat accggtcgcc ccggtccggt gatggctttc cgtgtcgata tggacgcgct
     8281 ggatctcagt gaagagcagg atgtcagcca tcgcccctac cgcgacggtt ttgcgtcatg
     8341 taacgccgga atgatgcatg cctgtggtca tgatggacat accgccattg ggcttgggct
     8401 ggcgcatacc cttaaacaat tcgagtccgg actacatggc gtcatcaaac tgatttttca
     8461 gcctgcagag gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg tcgtagatga
     8521 tgttgattat tttactgccg tgcacattgg cactggcgta cctgcgggca ccgtggtgtg
     8581 cggcagtgat aattttatgg caaccaccaa atttgacgcg cacttcaccg gtaccgccgc
     8641 tcacgcaggc gcaaaaccag aagacggtca caatgccttg ttggcggcag cacaagccac
     8701 tcttgcactg catgcaatcg ccccgcacag cgaaggagct tccagagtaa acgtgggcgt
     8761 tatgcaggca ggaagcggtc gtaacgttgt tcctgcctcg gcgttgctga aagtggaaac
     8821 acgcggggcc agcgacgtca ttaatcaata tgtttttgac cgtgcacaac aagcgattca
     8881 gggcgcagca accatgtatg gtgtcggcgt tgaaactcgt ctgatgggtg cagttaccgc
     8941 cagttctcct tcgccgcaat gggtcgcatg gttgcaaagt caggcggctc aggtcgcggg
     9001 ggtcaatcag gccattgaac gtgttgaagc gcctgcgggt tccgaagatg ccacattaat
     9061 gatggcccgc gtgcagcaac atcaagggca agcctcctac gtggtgtttg gcacacagct
     9121 ggcggcaggt catcacaacg aaaaattcga ttttgacgag caggttctcg ctattgccgt
     9181 cgaaacgctg gcgcgcaccg cgctcaattt tccctggacg cgaggtatct gatgcaggaa
     9241 atctatcgtt ttatcgacga tgcgattgaa gccgatcgcc aacgttatac cgatattgcc
     9301 gatcaaatct gggatcatcc agaaacacgt tttgaagagt tctggtcagc ggagcatctg
     9361 gcttcggctc tggaatctgc aggcttcacc gttacccgca acgtaggcaa tatcccaaat
     9421 gcctttattg cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg agaatatgac
     9481 gccctggcag gtttaagtca gcaagctggt tgcgcgcaac ctacatccgc gacgcccggt
     9541 gaaaatggtc acggttgcgg acacaatttg cggggaaccg ccgcctttgc cgctgcaata
     9601 gccgtcaaga aatggctgga acaatatggg caaggcggca cagtgcgctt ttatggttgt
     9661 cctggcgaag aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt atttgatgat
     9721 gtggatgcgg cactcacctg gcacccggaa gcctttgccg gtatgttcaa tacccgcacg
     9781 ctggcaaaca ttcaggcatc atggcgcttt aaagggatcg cagcacatgc cgcgaattcc
     9841 cctcatttgg gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg caccaacttc
     9901 ctcaacgaac atattattga aaaagcgcgc gtacactatg ccatcacaaa tagcggcggg
     9961 atctcgccca acgtggtcca ggcgcaggca gaagtgcttt atcttatccg cgcccccgaa
    10021 atgaccgacg tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg tgcggcattg
    10081 atgaccgaaa ccacggttga atgccgcttc gacaaagcct gttccagtta tctcccgaat
    10141 cgcaccttag aaaatgccat gtaccatgcc ctatcccatt ttggtacccc ggaatggaac
    10201 tccgaagaac tggcttttgc gaaacaaatt caggctacgc tcacccccaa cgatcggcaa
    10261 aacagtctga ataatatcgc cgcaaccggt ggcgaaaacg gcaaggcttt tgcactacgt
    10321 catcgtgaaa cggtactggc gaatgaggtc gctccatatg ccgccaccga taacgtgctt
    10381 gcggcatcga ctgatgtcgg cgacgttagt tggaaactgc ctgttgccca gtgtttcagc
    10441 ccctgttttg ccgtcggtac accgctacat acgtggcaac tggttagcca ggggcgaaca
    10501 tctattgctc ataaaggaat gctgctggcg gcgaaaacta tggcagcaac cacagtcaat
    10561 ctcttcattg attcagggct attgcaagaa tgccaacaag agcatcagca agtaacggac
    10621 acgcaaccgt atcactgccc tatcccgaaa aacgtgacac cgtcaccttt aaaataacaa
    10681 caacaacgca aacacaacaa ccgaggaatg cccatgagta tgtcatccat accgtcgtcc
    10741 tcccaatccg ggaagctcta tggctgggtc gaaagaattg gtaacaaggt tctccatcct
    10801 tttttgctct ttatctattt gattatcgta ctcatggtga cgacggcaat tttgtcggcc
    10861 tttggcgtca gtgcgaaaaa cccgaccgat ggaacgccgg tggtggtgaa aaacctgctc
    10921 agtgtggaag gattacactg gtctttaccc aatgttatta aaaactttag cggttttgct
    10981 ccacttggtg cgatcctggc gctggtttta ggtgccggtc tggcggagcg cgtcggctta
    11041 ctgccagcgc taatggttaa aatggcatcg catgttaatg cccgctacgc cagttatatg
    11101 gtgctgttta ttgctttttt cagccacatt tcttccgatg cgacgttagt gatcatgcta
    11161 ccgatgggtg cgctgatttt tctggcggtg ggcaggcatc cagttgcagg tttactggct
    11221 gccattgcag gcgtaggttg cggctttacg gctaatttac tgattgtcac aaccgacgtg
    11281 ttgctgtcgg ggatcagcac ggaagcggcg gctgcgttca atccgcaaat gcacgtcagt
    11341 gtaattgata actggtattt tatggccagc tccgtagtcg tactgacgat tgttggcggc
    11401 ctgataaccg acaaaatcat cgagccacgg ttaggtcaat ggcagggaaa cagcgatgag
    11461 aaactgcaga cattgaccga aagtcagcgt tttggtttac gcatagcagg tgtcgtatcg
    11521 ctacttttta ttgctgcgat tgcgctgatg gtgatcccgg aaaacgggat attgcgcgat
    11581 ccgattaatc acaccgtgat gccatcaccc tttattaaag gtatcgtgcc actgatcatt
    11641 ctttttttct ttgttgtctc gctggcttat ggcatcgcta cccgcacaat tcgacgtcag
    11701 gcggatttac cgcagttaat gattgaaccg atgaaagaga tggcaggatt tatcgtgatg
    11761 gtttttcccc tcgcccagtt tgtcgccatg tttaactgga gcaacat
//
LOCUS       DAZOYL010000110        11742 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZOYL010000110 DAZOYL010000000
VERSION     DAZOYL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.110"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            2..538
                     /locus_tag="ACHUOY_002189"
     CDS             2..538
                     /locus_tag="ACHUOY_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0682960.1"
                     /translation="MAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEIHQDDIDKEVE
                     LGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFDSSAKTTGADP
                     ILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFFAWMEQIDQAT
                     PVTPGAVTANATYVLDYK"
     gene            694..2726
                     /locus_tag="ACHUOY_002190"
                     /pseudo
     CDS             694..2726
                     /locus_tag="ACHUOY_002190"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2703..3047
                     /locus_tag="ACHUOY_002191"
     CDS             2703..3047
                     /locus_tag="ACHUOY_002191"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR0682961.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3062..3793
                     /locus_tag="ACHUOY_002192"
     CDS             3062..3793
                     /locus_tag="ACHUOY_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0682962.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3790..4851
                     /locus_tag="ACHUOY_002193"
     CDS             3790..4851
                     /locus_tag="ACHUOY_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0682963.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5003..6049
                     /locus_tag="ACHUOY_002194"
     CDS             5003..6049
                     /locus_tag="ACHUOY_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR0682964.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6046..6837)
                     /gene="nei"
                     /locus_tag="ACHUOY_002195"
     CDS             complement(6046..6837)
                     /gene="nei"
                     /locus_tag="ACHUOY_002195"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR0682965.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6873..7607)
                     /gene="pxpA"
                     /locus_tag="ACHUOY_002196"
     CDS             complement(6873..7607)
                     /gene="pxpA"
                     /locus_tag="ACHUOY_002196"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR0682966.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7597..8529)
                     /gene="pxpC"
                     /locus_tag="ACHUOY_002197"
     CDS             complement(7597..8529)
                     /gene="pxpC"
                     /locus_tag="ACHUOY_002197"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR0682967.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8523..9179)
                     /gene="pxpB"
                     /locus_tag="ACHUOY_002198"
     CDS             complement(8523..9179)
                     /gene="pxpB"
                     /locus_tag="ACHUOY_002198"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR0682968.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9202..9945)
                     /gene="ybgI"
                     /locus_tag="ACHUOY_002199"
     CDS             complement(9202..9945)
                     /gene="ybgI"
                     /locus_tag="ACHUOY_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR0682969.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10216..11697
                     /gene="dtpD"
                     /locus_tag="ACHUOY_002200"
     CDS             10216..11697
                     /gene="dtpD"
                     /locus_tag="ACHUOY_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR0682970.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2804 a         3145 c         2884 g         2909 t
ORIGIN      
        1 actggccgta tctctgctgt tcacagcacc tgtttatgcg gctgatgaag gatccggtga
       61 aattcacttt aaaggtgaag ttattgaagc accgtgtgaa atacatcagg atgatattga
      121 taaagaggtt gaactcggtc aggtgaccac cagccacatt aatcagtcac atcacagcga
      181 tgccgttgct gtcgacctgc gcttagtcaa ctgtgatctg gaaaactcca gcaacggttc
      241 cggtggcaag atttctaaag ttgcagtcac ctttgatagc tcagcgaaaa ccaccggcgc
      301 agatccgatt ctcaacaaca ccagcacggg tgaagccacc ggcgtcggcg tacgtttaat
      361 gaataaagat caaagcaata tcgttttagg caccgctact ccagatatcg acctggctcc
      421 gacctccagc gaacaaacgc tgaatttctt tgcctggatg gaacaaattg atcaggctac
      481 acctgtaacg ccaggcgcag ttacggcgaa tgcaacctac gtgctggatt ataaataagt
      541 attactttgg cggcgctatc gccgcctttc ataaaatatt tatcaggtat ggacaccgtg
      601 aatatttatc gactctcttt tgtatcctgc ctggtcgtgg cgatgccttg cgcattggcg
      661 gtcgaattca accttaatgt tctcgataaa tcgatgcgcg accgcattga tatttcatta
      721 ttaaaggaaa aaggagtcat tgctcccggt gagtattttg ttagcgttgc ggtaaataat
      781 aaccaaatca gtaacgggca aaagattaac tggcacaaaa atgacgataa aaccattccg
      841 tgcatcaatg atttactggt cgataaattt ggcttaaaac ctgaagtccg tcagtcgtta
      901 ccattgataa atcagtgcgt cgattttagc tcccgacctg aaatgctctt caatttcgat
      961 caagccaatc agcaactgaa tattagtatt ccgcaagcct ggctggcgtg gcactcagaa
     1021 aactggaccc caccctccac atggaaagaa ggtgtcgccg gtatcctgat ggattacaac
     1081 ttgtttgcca gcagctaccg cccacaggac ggcagcagca gcactaacct gaacgcctac
     1141 ggtaccaccg gaattaacgc cggggcatgg cgcttacgta gtgattatca gttgaatcag
     1201 actgatagcg atgataacca tgaacagtca ggcggaatat cgcgcaccta tctttttcgt
     1261 ccattaccgc aattaggctc taaattaacc ctcggcgaaa cggattttag ttccaatatt
     1321 ttcgacggtt tttcttatac cggcgcggca ctggcaagtg acgagcgaat gttgccatgg
     1381 gaactacgcg gctacgcccc acaaattagc ggtattgcac agaccaatgc cacggtgacg
     1441 atcagtcaat caggccgcgt catttaccag aaaaaagtcc caccaggccc atttatcatt
     1501 gacgacctta atcagtctgt tcagggcaca ctggatgtca aagtgacgga agaagatggt
     1561 cgggtgaaca atttccaggt ttcggcagca tcgacgccct tcctgactcg tcagggacag
     1621 gttcgctata aactggccgc gggtcagcca cggccctcca tgtcacatca aactgaaaat
     1681 gaaacctttt ttagcaatga agtttcctgg gggatgctgt caaacacctc gctgtacggc
     1741 ggcctgctgc tttctggtga tgactaccat tctgccgcaa tgggtattgg gcaaaatatg
     1801 ctgtggcttg gtgcgctgtc gtttgatgtc acgtgggcca gtagccattt tgatactcag
     1861 caggacgagc ggggcttaag ctaccgtttt aattacagca aacaagtgga tgccactaac
     1921 agcacgattt ccctcgccgc ttatcgtttc tccgatcgtc attttcacag ctacgccaac
     1981 tatctggatc acaaatacaa cgacagcgat gcgcaggacg aaaaacagac gatcagctta
     2041 ttgtgggcca accgattacc ccactaaacc tcaatcttta cgccaacctg ctacatcaaa
     2101 cctggtggaa tgcagacgcc tccacgaccg ccaacatcac ggcaggtttt aatgttgata
     2161 ttggtgactg gagagatatc tctatttcga cgtcattcaa tacaacccat tacgaagata
     2221 aagatcacga caaccagatt tacctgtcga tttcgctccc cttcggtaac ggtggtcggg
     2281 ttggctatga catgcaaaac agtagccaca gcaccacaca ccgcatgtcg tggaacgata
     2341 cgctggatga acgtaatagc tggggcatgt ctgccggact gcaatccgac cgtcctgaca
     2401 atggagccca ggtgagcggt aactatcagc acctgagttc agcgggtgag tgggatattt
     2461 ctggtaccta tgccgccaat gattacagtt ccgtcagcag cagctggagc ggttctttca
     2521 ccgcaaccca atatggtgca gcgtttcatc gccgcagctc caccaatgaa ccacgcctga
     2581 tggtcagcac cgatggcgtg gcagatattc cggttcaggg caatctcgac tacaccaacc
     2641 attttggcat tgcggtggtg ccgttgattt ccagttacca gccttccacc gtggcggtga
     2701 acatgaatga cttacccgac ggcgtaacag ttgcagaaaa cgttatcaaa gaaacgtgga
     2761 ttgaaggcgc gataggttac aaatcactgg cttcccgttc cggtaaagac gttaacgtca
     2821 tcattcgcaa cgccagcggt cagtttcctc ccctcggagc ggatatccgc caggatgaca
     2881 gcggcattag cgtggggatg gttggcgagg aaggacatgc ctggttaagc ggagtcgctg
     2941 aaaatcaaaa gtttaccgtg gtctggggtg atagccagca ttgctcgctc catcttcctg
     3001 aacatatgga agacaccgca aatcgcctga ttttaccttg tcattaatta aggaaatgaa
     3061 aatgacattt atgaaaggac tacccctttt gttgttggtt gccagtctgt gcagccacgc
     3121 tgcactacaa cccgatcgca ctcgtattgt gtttaacgct aatgataaag ctaccagtct
     3181 gcgggtggat aatcgtagcg ataaactccc ctacctcgct tattcctggc tcgaaaatga
     3241 aaaaggggag aaaagcgatg atcttctggt cgccctgccg ccgatacaac gtctggaacc
     3301 gaaagcaaca acgcaggtac ggatagtaaa gcaagcctcg accacaaaat taccgggcga
     3361 tcgcgaaacg ctatttttct acaatatgcg tgaaattcct cccgccccgg aaaaaaacag
     3421 cgaccatgcc gttctgcaag tcgccattca gagtcgtatc aaggtgttct ggcgaccggc
     3481 tgcattacgc aagaaagcag gagaaaaggt cgaactgcag ttacaggtca gccagcaggg
     3541 caaccaactg acactgaaaa atcctaccgc gtattatctg accattgcct acctgggacg
     3601 aaatgaaaaa ggcgttctcc ccggcttcaa aaccgtgatg gttgcgcctt ttagtacggt
     3661 caacacaaac accggaaatt atagcggcag ccagttttat ctcggttata tggatgatta
     3721 cggcgcgtta cgcatgacga cgctcaactg cagcggacaa tgccatttac aagccgtgga
     3781 ggcgaagaaa tgagtgctgg caagggagtg ttgctcgtca tctgtctgtt atttctgccg
     3841 ttgaagtctg cgcttgccct gaactgctac tttggttcat ccggtggctc ggtagaaaaa
     3901 tcagaagcga ttcaaccttt tgctgtacca ggcaatgcca aactcggcga taagatttgg
     3961 gaatctgacg atattaaaat tcccgtctat tgtgacaaca ataccaacgg caattttgaa
     4021 agtgagcatg tttatgcctg ggtaaacccc tacccggggg tgcaggatcg ctattatcaa
     4081 ctcggcgtaa cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac
     4141 actaaccagt gtattgacag taagaacatt gacatttata cccctgagca gattatcgcg
     4201 atgggatggc agaacaaaat ttgctcgggt gatcccgcta atattcacat gtcgcgtacc
     4261 tttctcgctc gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca tgattatcaa
     4321 agtacgctta gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt taacgaagac
     4381 cctactgccc aaaacctgaa atatcatatt actggtctgg aaaacattcg cgtgctggat
     4441 tgcagcgtca atttttccat ttcaccggaa acacaggtga ttgattttgg taaatttaat
     4501 ttgctggata ttcgtcgcca cacaatgtcg aaaacattca gtattaagac gacaaaatca
     4561 caaaatgacc aatgcaccga cggatttaaa gtcagttcct ccttttatac cgaagaaacg
     4621 ttggttgaag aagataaagc attgttgatt ggtaatggtc tgaagctacg tttgctggat
     4681 gaaaacgcct cgccttatac cttcaataaa tatgctgaat acgccgattt caccagcgac
     4741 atgttggtct acgaaaaaac ctatacagct gaactttcgt ccattgcagg tacccccatc
     4801 gaggctgggc ccttcgatac agtggtgctt tttaaaatta actataactg atttgccatg
     4861 atgccctttg cagccggaaa gggcatctct ttcagaaaat tgctgatttt ccccttttcc
     4921 ttgattgctg ttattttttc ctggcccggc tttctttatg ctgtatcgat acaccaaaga
     4981 gaaaataatg agggagcgaa ggatgccagt tttgcagtgg ggaatgttat gtgtgctatc
     5041 acttctcctt tctattggtt tcctcgcggt acatctcccg gcggcgctat tacttgggcc
     5101 gatgatcgcc gggatcatct tcagtatgcg cggaattact ctgcaacttc cccgctccgc
     5161 ttttctcgcc gcgcaggcca ttcttggctg catgattgcg caaaacctca ctggttcgat
     5221 tctcaccacg ctggccgcca actggccgat cgtgcttgct attttactgg tgacgctgct
     5281 ttccagcgcc atcgtgggtt ggttattggt gcgctatagc tcactgcctg gaaataccgg
     5341 tgcctggggt tcttctccag gcggcgcagc ggcaatggtc gctatggcgc aagattacgg
     5401 cgcagatatt cgcctggttg catttatgca atatctgcga gtgctgttcg tcgcgggggc
     5461 cgctgttctg gtcacccgca tgatgctggg tgataacgct gaagcggtta accagcaaat
     5521 cgtctggttc ccgccagtga gcattaatct cctgcttacg attttgctcg cagtcgttgc
     5581 cggtacggcg ggatgcatgc tgcgtcttcc ttccggcacg atgctcatcc cgatgctggc
     5641 gggcgcagtg ctccagtctg gtcagctcat caccatcgaa ctaccggaat ggctgctggc
     5701 gatggcgtat atggcaattg gctggcggat tggtcttggt ttcgataaac aaatcttact
     5761 gcgggcacta cgcccgctac cgcaaatcct gctgtcgatt tttgctctgc tggctatttg
     5821 tgcgggtatg gcgtgggggc tgacccggtt tatgcacatt gattttatga ctgcctacct
     5881 cgccaccagt cccggcgggc ttgataccgt ggcggtcatc gccgcaggga gcaatgccga
     5941 tatggcgctt attatggcga tgcaaaccct gcgcctgttc agtattttgc tgacggggcc
     6001 agccattgcg cggtttattt caacctatgc gccgaagcgg tcggcctagt gctggcagcc
     6061 agggcaccag taaaacgggc gagatgacag cgtggttttc tcaatgatgc tgccacagcg
     6121 ttcgcacggt tcgccatcac gatgaaaaac cttaaagcga aacagcgccc catgatgctt
     6181 attctcatcc accagccccc tcgtagcgta ggaaaatcga ggaatatcca gtaacgcgtg
     6241 tgcgagtgca tccagttgcg ccgcattgag atctttcgct ttatgatttc cagtcaaccc
     6301 aacctgccag aggatctcca cccgcaaata attgccaagc ccagccagaa acgcctgatc
     6361 gagcagtaat cctgcaaact gacggttacg aaagcgcggt gacaataacc gttcctttac
     6421 cacctccggc gtcagattcg gatccagcac atcgggacca acgcgttgta aaaacggatg
     6481 cgtggtcagt tgttccggcc tcaacatctc aatatccgag gcgctataaa gcagaatggt
     6541 tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg gtctgcggct cttcgccggt
     6601 atcaaccacg cgccagacgc cgtaaagctg attatggctg tagagcgtta agtcgttgga
     6661 aaaatgagtt aacaacgcct taccacgcgt ttccacatgg gtaacgtgtt gaccgataag
     6721 ttgtgattga taagttttta actgcgggaa ggcaaaccag acatcagtta gtggtttgcc
     6781 tttgatcgcc gcctccaggt tatccgctgc acggcggatc tccgggcctt caggcatggt
     6841 gatatccttt tttcattctt tattaatcgg ggttatgctg cgacaacaat ctccttttcg
     6901 gcaaatgtag agcgtagtcg gcgggcgaac gccagtgcgt gctcgccgtc gccatgcagg
     6961 cagactgttt gcgccgtgac cgttgcccat tcgccggtga tacttttgac tctgccgtgt
     7021 tgcaccatct ctagcgtttg cgccaacgcc tgttcttcgt tttcaatcaa cgcgcctcgc
     7081 tgacttcgcg gcaccagcga gccgtcagcc tgataaccgc gatcggcaaa cacttcctcg
     7141 cgcgttgtca ggccatattg cttgcctgca cgaatcagct cgcttcctgc cagcccaacg
     7201 agaatcaatg ccggatcgca agcgtatacc gctctggcga tggcgtctgc cagttgtgct
     7261 tctttcgccg cctggttgta caacatgccg tgcggtttga catgacgcat tacgccgcct
     7321 tgcgcacggg caatcgttgc cagcgcgcca atttgataca gcgtctgggc gtaaacggtt
     7381 tctggcggca gctgcatggc gctgcgacca aaattttccc tgtcgggaaa actcgggtga
     7441 gcgccaatcg cgacaccatt ttttattgct tcacgcacgc aagcctgcat gatttgcgca
     7501 tcgcctgcat gaaatccaca ggcaatattg gcagaggaaa ccagcgttaa tagctctgcg
     7561 tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa ttttcattgt gcagccgcca
     7621 cactaattgt tcgaaataac gttgctgatc ttgccgcgct ttcagtgcct cttccagtga
     7681 acactggaca aaatgaatcg gctgaccgag cggaatttgc gccagatggt acatatcagc
     7741 ctcaatgata caggcaatac gcgggtaacc atcggtggtc tgtgcgtcgt tcatcaacac
     7801 aatcggctgc ccgttatgtg gcacctgcac cacgcccggt aacaaaccgt gagataacag
     7861 ttcgcgatcg gtggtgcgtt ttaaaatttg cccctgtaag cgatagccca tgcggttact
     7921 ttgcgagcta agctgccagg gcgaacgcca gaatgcatcc tgcgaggcgc gatcgaactc
     7981 atgatattcc ggccccggca aggcgcgaat gcggttgccc cacagcagct gtttaacgcc
     8041 ctgcgcttcc ataaaatcac gcttcgattt gccaatcggg agtcggtcac catccttcag
     8101 taaacggcct tccagcccgc caatccccac tttgagatcg gtgctgcatg accccattac
     8161 cggcggaaca tcaataccac ccgcgaccgc cagataactg cgcatcccgt gctgcgggcg
     8221 tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc cagccggtcc agacggcatt
     8281 atcatccagc cgcgcttcgc aaccggcacc cgtcagagca aaccacccat cagtttcgaa
     8341 ctcaaccgtt aactgaccga gcgtgatctc cagcgcgggg gcattggcgt cattacccac
     8401 cagcaggtta gcaatgcgta acgcaggcat atccagtgcg ccgcagtggc tgattcccga
     8461 ctggcgaaaa ccgtgacgac cgccatcctg cacagtggta tacatgcccg cacgaataat
     8521 cttcagcata ctccctcctt ctgcggtaca aagcgcacgc tgtctcccgg acgtaataag
     8581 atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg tatgaccaat caactgccag
     8641 ccacccggcg ttgccagcgg ataaacacca gtctgcggcc cgccgatccc gacagaacct
     8701 gccggaacga gcaagcgcgg ttcagcgcgc cgtggcgtgt gtagttgttc cggcaaactc
     8761 ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga ccacgtattc cacggaggag
     8821 tgcaattcaa caacctgttt ttcgctaaac ccgcaatgcg ccgcgaccac cgccaaatcc
     8881 ggtccgcctg caccaccgta aaccaccgga atttcaataa agcgagactc aggttccagg
     8941 gcctcgctct cctcccacca gcgttgcaaa cgctctatgg catccagcgc cagcgactca
     9001 ggattacgca aaatcaccgt gatattgttc atgccgggaa tggcttcaac cacattcggc
     9061 atatccacca gacgctgcgc cagtcgccag atccgtttct ggctagccag cgtcaccggc
     9121 ggttccagtt ccagcactac cgccgtttca cctatcagat aacaacgcgc tcgttgcact
     9181 ttcgatccct ctgattattc gttatgcagg attaggaata tcaataaagg tcacatcaag
     9241 atcggtattt tcattcagcc actcgctcaa tgcgcgaata ccaccacgtt cagtggcatg
     9301 gtgacctgca gcataaaaat gcaacccctg ctcgcgggct gaatgaatgg tctgttcaga
     9361 aacttcgcca gtaataaaag catccacgcc aaaacgcgcg gcgctatcga taaaactttg
     9421 cccgccgccc gtgcaccagg caacgcgctg gaccacctca ggtccggtat cgccacacca
     9481 taacggctta cgtcccagac gcgcttcaat ccaggaggct agttccagtc ccggcacagg
     9541 catggtcagc tctccccacg gcaccaacgg ctcaatttcg cccatgaccg tgatccccag
     9601 taacgccgcc agctgtgcgt tattgcccag ctcaggatgt acgtcaagcg gcaaatgcca
     9661 gccatacagg ttgatatcat tcgccagcaa cgtttttaaa cggttacgct tcattccgcg
     9721 aatgaccgga gactctcctt tccagaagta gccatgatgc acaatgaccg catccgcgcc
     9781 cagacgcact gcctcatcga gcaaagcctg gctggcggtg acaccggtaa caattttttg
     9841 caccgtctct ttgccttcta cctgcaaacc attcggcgcg taatcactaa tcgccgcgct
     9901 gttcagtttt tcgttaatca gttgttccag ttcggtgttt ttcatcttcg cgccttccgt
     9961 attctgatag ctacaccgtt aacatagcgt tacctcgcct gttcgtcgat agccaatctt
    10021 ttgacatatt aagaaacaaa cccgacgcca aaagacgcaa attgtttata tcatcacgct
    10081 tcagctaatg tatatttatg caatcagaca gcatattgca taaataatca gatctgtatc
    10141 aaagttttaa ctttaaggaa acacaagcac gccgcgcaac tattacgtga cgttgcagtc
    10201 aattgtggaa ttattatgaa taaacacgca tcacaaccgc gcactattta ctatgtcgtt
    10261 gcgctgcaaa tctgggaata ttttagcttt tacggcatgc gtgccctgct gattctctat
    10321 ctcaccaatc aactaaaata caacgatact cacgcctacg agttatttag cgcctactgt
    10381 tcgctggtgt atgtcacgcc aatcctcggt ggctttttgg cggataaagt tctcggcaat
    10441 cgcatggcgg tgatgctggg ggcgttgttg atggcgatcg gtcatgtggt gctgggtgcc
    10501 agtgagatcc atccgtcatt cctctatctg tccctggcga ttatcgtttg tggctatggt
    10561 ctgtttaaat ccaatgtcag ttgcctgctc ggcgagctgt atgagccaac cgatccgcgt
    10621 cgtgatggcg gtttctcgct gatgtatgca gcgggtaacg tggggtctat tatcgcacct
    10681 atcgcctgtg gttacgccca ggaagagtac agctgggcga tgggctttgg cctggcggcg
    10741 gtgggtatga tcgcgggtct ggtcattttc ttatgtggca atcgtcattt cacacatacc
    10801 cgcggcgtta ataaaaaagt actgcgtgca acaaactttc tcctgcctaa ctggggatgg
    10861 ctgctggttc tgctggtggc aacgcctgcg ctgattaccg tactgttctg gaaagagtgg
    10921 tcggtatacg ccttaattgt cgcgactatt attggcctgg gtgtactggc aaaaatttat
    10981 cgcaaagcag aaaaccagaa acagcgtaag gagctggggc tgattgtgac gctcaccttc
    11041 ttcagtatgt tgttctgggc cttcgcacaa cagggcggca gctcaattag cctttatatc
    11101 gaccgcttcg ttaaccgcga tatgtttggt tataccgttc cgactgctat gttccagtcg
    11161 attaatgcct tcgcagttat gctatgcggt gtgttcctgg cgtgggtggt taaagaaagc
    11221 gtcgcaggta atcgtaccgt gcgcatctgg ggaaaatttg ctcttggcct tggcctgatg
    11281 agcgccggat tctgcattct gaccttaagc gcccgctggt ccgcaatgta tggtcactct
    11341 tctctgccac tgatggtgtt aggcctcgcg gtgatgggct ttgcggaact gtttatcgac
    11401 ccggttgcca tgtcgcaaat tacgcgcatt gaaattccgg gcgtgaccgg cgtattaacc
    11461 ggcatctaca tgctgctttc tggcgcgatc gcgaactatc tggcgggcgt gattgccgat
    11521 cagacatcgc aggcttcgtt tgatgcttcc ggggcgatca actactccat caatgcatat
    11581 attgaagtat ttgatcaaat tacctggggc gcactggcgt gtgtaggact ggtactgatg
    11641 atttggctgt atcaggcgct gaaattcaga aaccgcgcgc tggcgctgga gtcataatag
    11701 gccagcaccg atgcctgatg cgacgcttga cgcgtcttat ca
//
LOCUS       DAZOYL010000111        11657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZOYL010000111 DAZOYL010000000
VERSION     DAZOYL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.111"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            77..1154
                     /gene="ygjJ"
                     /locus_tag="ACHUOY_002201"
                     /pseudo
     CDS             77..1154
                     /gene="ygjJ"
                     /locus_tag="ACHUOY_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1171..3522
                     /gene="ygjK"
                     /locus_tag="ACHUOY_002202"
     CDS             1171..3522
                     /gene="ygjK"
                     /locus_tag="ACHUOY_002202"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR0682971.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            3748..5766
                     /gene="fadH"
                     /locus_tag="ACHUOY_002203"
     CDS             3748..5766
                     /gene="fadH"
                     /locus_tag="ACHUOY_002203"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR0682972.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(5811..6227)
                     /gene="higA"
                     /locus_tag="ACHUOY_002204"
     CDS             complement(5811..6227)
                     /gene="higA"
                     /locus_tag="ACHUOY_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR0682973.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6224..6538)
                     /gene="higB"
                     /locus_tag="ACHUOY_002205"
     CDS             complement(6224..6538)
                     /gene="higB"
                     /locus_tag="ACHUOY_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR0682974.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(6823..7959)
                     /gene="rlmG"
                     /locus_tag="ACHUOY_002206"
     CDS             complement(6823..7959)
                     /gene="rlmG"
                     /locus_tag="ACHUOY_002206"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR0682975.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8044..8547
                     /locus_tag="ACHUOY_002207"
     CDS             8044..8547
                     /locus_tag="ACHUOY_002207"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR0682976.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            8624..9307
                     /locus_tag="ACHUOY_002208"
     CDS             8624..9307
                     /locus_tag="ACHUOY_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR0682977.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9386..10372
                     /locus_tag="ACHUOY_002209"
     CDS             9386..10372
                     /locus_tag="ACHUOY_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0682978.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            10655..11620
                     /gene="alx"
                     /locus_tag="ACHUOY_002210"
     CDS             10655..11620
                     /gene="alx"
                     /locus_tag="ACHUOY_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR0682979.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2769 a         3007 c         3135 g         2746 t
ORIGIN      
        1 atagtttctg cctttatatt tttataatta caacgataaa aggctgtact ttttctttag
       61 ctcatggatt aacacaatga aattaatcac tgcaccatgc agagcattac ttgctctgcc
      121 gttttgctac gccttttctg cggcaggaga agaagcacgt ccggcagaac atgacgacac
      181 aaaaacaccc gcaattacct cgacatcttc tccttcattt cgtttttacg gcgaattagg
      241 gattaggggg tggtggatat atggatttag agggtgagaa taaacataaa tacagcgacg
      301 gtacctatat tgaaggtggc ctggagatga agtacggctc ctggttcggc ctgatttacg
      361 gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc ctgggtgcca gaccatagct
      421 ggggtggttt cgagggcgga attaaccgtt tctatggcgg ttatcgtacc aatgatggca
      481 ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga tgacctgcaa tggtggggcg
      541 atttcacccc cgatctgggc tacgtcattc ccaatacccg cgacattatg actgcgctga
      601 aggtacagaa cttaagcggc aactttcgtt atagcgtcac cgcgactcct gccggacatc
      661 atgatgaaag caaagcctgg ctacattttg gcaaatacga tcgctatgac gacaaataca
      721 cctatccggc aatgatgaac ggttacatcc agtatgacct tgccgaaggc atcacctgga
      781 tgaacggtct ggaaatcacc gacggcacag gacagctcta tctcacgggc ctgctaactc
      841 ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc cgacgggctg gacgtaccgg
      901 gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc gttaaagggc gtttatctct
      961 ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc tgacgatgaa accacctctt
     1021 tcatgcagtt tggtatctgg tacgaatacg gcggcggacg tttcgccacg gcttttgata
     1081 gccgcttcta catgaaaaat gcctctcacg atcccagcga ccaaatcttc ctgatgcaat
     1141 atttctactg gtaataagga ctgtaatttc atgaaaataa aaactatttt aacgccagta
     1201 acctgcgctc tgctgataag tttttccgcc catgccgcta acgccgacaa ttataaaaac
     1261 gtgattaacc gtactggcgc gccgcagtac atgaaggatt acgattacga cgatcaccag
     1321 cgttttaatc cgtttttcga tctcggagcc tggcatggtc atctgttgcc agacggccct
     1381 aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg aagagtacat caactttatg
     1441 gccagcaatt tcgaccgcct gaccgtctgg caggacggca agaaagtcga ctttacgctg
     1501 gaggcataca gtattcccgg tgcgctggtg caaaaactga cagcaaaaga tgtgcaagtc
     1561 gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac tggaaaccaa aatcaccagc
     1621 aataaaccgc tggatctggt gtgggacggc gaactgctgg aaaaactgga agcgaaagaa
     1681 gggaaaccgc tttccgataa aaccattgct ggcgaatacc ctgactatca gcgcaaaatc
     1741 agcgccaccc gtgatggcct gaaagtcacc tttggcaaag tgcgcgccac ctgggatctg
     1801 ctgacctccg gcgaatcgga atatcaggtg cataaatccc tgccggtgca gactgaaatc
     1861 aacggtaatc gctttaccag taaggcgcat atcaacggtt cgaccacgct ctacaccacc
     1921 tattcccatc tgctgaccgc tcaggaagtt agcaaagagc aaatgcagat ccgcgatatt
     1981 ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac gctgggaaga atacctgaag
     2041 aaagggttaa ccaatccgga tgcgacgccg gaacagacgc gcgtcgcggt gaaagcgatc
     2101 gaaacgctca acggtaactg gctctcaccg ggcggtgcgg tgaaatttaa caccgttaca
     2161 ccgtcggtga ccgggcgctg gttctccggc aatcagacct ggccgtggga tacctggaag
     2221 caggcgtttg cgatggcgca tttcaatccg gacatcgcca aagagaatat ccgcgcggtc
     2281 ttctcctggc agatccagcc tggcgatagc gtgcgtccgc aggatgtggg ctttgtcccc
     2341 gacctgatag cgtggaacct tagccccgag cgtggtggcg atggcggcaa ctggaacgaa
     2401 cgtaatacca aacccagcct tgccgcctgg tcggtgatgg aagtgtacaa cgtcacccag
     2461 gataaagcct ggttggcaga gatgtacccg aaactggtgg cctatcacga ctggtggtta
     2521 cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg gcgcgacccg cgacaaagcc
     2581 cacaacactg agagcggcga gatgctgttt acggtgaaaa aaggcgataa agaagagacg
     2641 cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag gcctgtacga cagtctggaa
     2701 attccggcac aggttgctgc atcgtgggaa tcggggcgtg atgatgccgc cgtctttggg
     2761 tttatcgaca aagaacagct ggataaatat gtcgctaatg gcggcaaacg tagcgactgg
     2821 acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt tgctgggcta ctcgctattg
     2881 caggagtcgg tggatcaggc cagctatatg tacagcgata accattatct ggcggagatg
     2941 gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc gccagttggc acagcagctc
     3001 gcggactaca tcaacacctg tatgttcgac ccgactaccc agttctacta tgacgtacgt
     3061 attgaagata aaccactggc gaacggctgc gcgggcaaac cgattgttga gcgcggtaaa
     3121 gggccggaag gctggttgcc gctgtttaac ggtgcggcaa cgcaggccaa cgccgacgcg
     3181 gtggtgaagg tgatgctcga tcctaaagag ttcaacacct ttgtcccgct gggaacggcg
     3241 gcgttaacca atccggcttt tggcgctgat atctactggc gtgggcgcgt atgggtggat
     3301 cagttctggt ttggtctgaa agggatggag cgttacggtt atcgtgatga tgccctgaag
     3361 ctggcggata cgttcttccg gcacgccaaa ggattaaccg ccgatggccc aattcaggag
     3421 aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa atttctcctg gagtgccgct
     3481 catttgtata tgttgtataa cgattttttc cgtaagcagt aatttgcaac ggctggcgga
     3541 tgcggcgtga acgccttatc cgccctacat gtgcaatccc ccaggccgga taaaacgcgg
     3601 caagcgttgc atccggcgtt accaccgggc gtattctttt tgaatcccat cacaaacccc
     3661 gcactcccct tttccctttt ctccatcgac ggctaaatta gaactcatcc gaccacataa
     3721 caattatttt acatactgga caattttatg agctatccgt cgctgttcgc cccgctggat
     3781 ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct caatgcacac cgggctggag
     3841 gaatacccgg acggtgccga gcggctggca gcgttttatg ccgaacgcgc ccgtcacggc
     3901 gtggcgctga ttgtcagcgg cggcattgca ccagatttaa caggcgttgg catggaaggt
     3961 ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc gcaccattac cgaagcggta
     4021 catcaggaag gcggcaaaat agccctgcaa attttgcata ccgggcgcta cagctaccaa
     4081 ccgcatctgg tcgctccctc cgcattgcag gcccccatca accgtttcgt tcctcacgaa
     4141 ctcacccatg aagagatcct gcaactgatc gacgatttcg cccactgcgc gcaactggca
     4201 cgggaggcag gatacgacgg tgtagaggta atggggtccg aagggtattt gatcaacgaa
     4261 tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg gcggcgatta ccgcaaccgg
     4321 atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac gcgtcggcaa cgacttcatt
     4381 attatctacc gactgtcgat gctcgacctg gtcgaagacg gcgggacttt tgccgaaacg
     4441 gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca ttatcaacac cggcattggc
     4501 tggcatgaag cacgtattcc gaccattgcc acgcccgtgc cgcgcggcgc atttagctgg
     4561 gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg taaccaccaa ccggattaac
     4621 gatccgcagg ttgccgacga tattctctcg cgcggcgatg ccgatatggt atcgatggca
     4681 cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc aatcggggcg agccgatgag
     4741 atcaacacct gcattggctg caatcaggcc tgcctcgatc agatcttcgt tggcaaagtc
     4801 acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca aaatgccaat ccttcctgcc
     4861 gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg ctgggctggc gtttgccatt
     4921 aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg cccacagcga gatcggcggg
     4981 cagtttaata tcgccaaaca gatccccggc aaagaggagt tttacgaaac gctgcgctat
     5041 taccaccgga tgatcgaagt gacgggcgtg acgctgaaac tcaatcacac cgtgacggcg
     5101 gatcagttac aggcgttcga tgaaacgatc ctcgccagtg ggatcgtgcc gcgcgttccg
     5161 cctatcgacg ggatcgatca tccgaaggta ttgagttatc tcgatgtact gcgcgacaaa
     5221 gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg ggattggttt tgatacggcg
     5281 atgtatttaa gtcagccggg cgaatccacc agccagaata tcgccaggtt ctgtaatgag
     5341 tgggggatcg acagcagcct acaacaggct ggtggcttaa gcccgcaggg aatgcagatc
     5401 ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag ccagcaaacc gggacagggg
     5461 ttaggcaaaa ccaccggctg gatccatcgc accaccctgc tctcgcgggg tgtgaaaatg
     5521 atcccaggcg taagttatca gaagattgac gatgacgggc tgcatgtggt gatcaacggc
     5581 gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg cagggcaaga gccaaaccgc
     5641 gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc atttaattgg cggctgcgat
     5701 gtggctatgg agctggacgc acgacgggca attgcccagg gaacacggct ggcgctggag
     5761 atttaaatcg ttattttaag cgccggatgc aacgcatccg gcacgtcgcg ttaatcaata
     5821 aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt tagcgtgttc cagcgttaat
     5881 ttcctcttcc cgctcaaaac gcgtgacacc atagatttac tgccaatttc cggcagatcg
     5941 gcaagggtta aaccatattg atccataagg gtacgaatca cggctatacc gccaggcatg
     6001 gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg attcttccca cgcggttatt
     6061 ttggcacaca ccagatccag caaggggttt tcaggatcgt tgagcagcag atgatctacc
     6121 agttccagcg cctgggtgta ttgttcctcg ttctgaatac ccgccagaaa aggtgccacc
     6181 gcagttagct tttctcctgc ttgcaagatg tcggcaatcg caatcatttt ttccccttag
     6241 tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt cataacttca cgtatgtagc
     6301 acttttgcga ttcaaaaaag accattgcta caacacgtaa ttcattgccc ccaacattga
     6361 aaacataatg cttatccaga tatttgaagt tatccagaga tgggaatact gcttttaatg
     6421 actcaggttt tttgaaatat cccttagcaa tcgtgttccc cagagccacc aactccgttt
     6481 tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt ttgagttatc aggtgcattc
     6541 ttcatcacgt ccgttgacaa attggcaata tgataacatc cgttgacaga ttggcaacgg
     6601 atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa cgctaaacaa gagaatgtag
     6661 aggatggtga gagggatttt ttgttttatg gaagcgcgat tccagaactg ggcagacatt
     6721 gccggatgcg acgcttgccg cgtcttatcc ggcctgggag taacgtgctg aacgtaggcc
     6781 ggataaggcg ttaacgccgc atccggcagg taactcccaa tcttaccgac gacgccccag
     6841 cttcactgct ttcagcacca caaatttatt attcgtggcg atggtggtgc agttgccgaa
     6901 aatcttcttc agtttatgga agtaatccag gtgacggttg gcaacaatat acagctcgcc
     6961 gttgattttc aggcagcggc gggcatggtg gaacatctcc caggcgacgt tatcggtcag
     7021 cgcatgttgt tggtgaaacg gcgggttgca gagcacagca ttaaagcgga aaggctccac
     7081 gccggagagc gcgttgttga tcataaactc gctgcgatct aacgcctctg gcatgttggt
     7141 ttcaacgttc aaacggctgg aagcaaccgc catcggcgat tcatcgacaa acaccacttt
     7201 cgcctgaggg tttttatcaa gcagcgtcag accaataacg ccattaccgc aaccgagatc
     7261 gacaatctca ccttcgagat tctctggcag atgttgcata aagaagcgtg cgccaatatc
     7321 cagcccggtg cgggagaaga cattcgcatg gttgtggata gtccagtcag taccttccag
     7381 cttccagcta acggtctgcg gcgcatcggc cagcggcggt tcattgaaag tgcaattaat
     7441 caggcgcgct tttttccatg ccagcgtagt ggtggtcggg ccgagcactt tctcgaacag
     7501 ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg gcaataatac gtgtatccga
     7561 cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc aatgccagtg ttttcggcac
     7621 tttgatcagc accacacccg gctgttgcgg gtagtcggcg gtgctgtcga gaaacttcac
     7681 gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc gtcgccagtt cactgatgta
     7741 tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg cagcttaacg caccaaaggc
     7801 atcattcagg atcaacaccg ggccgcggat ttctgtgtcg tccaactgtt gcagcaaata
     7861 ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca tccgtcgccg gaaaacgttg
     7921 tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc ggccctcctg aatgataaaa
     7981 tttcgacgtt gcccctgaaa agggtgcgtg agtataccct ttttctcttt ttcgtgggcg
     8041 gttatgagca atcttactta tcttcagggt tatcccgagc agctactttc ccaggtgcga
     8101 acgctgatta acgaacagcg tctgggcgat gtgctggcaa aacgctatcc gggaacacac
     8161 gactacgcca ccgataaagc cctctggcaa tatacccagg atctaaaaaa tcagtttctg
     8221 cgcaatgccc cgccgatcac taaagtgatg tatgacaata agatccacgt actgaaaaac
     8281 gcgctcggat tacataccgc tgtttctcgt gtgcagggcg gcaagctgaa agcaaaagca
     8341 gagatccgcg tcgctactgt gtttcgcaac gcgccggaac cgtttttacg catgatcgtg
     8401 gtgcacgaac tggcgcacct gaaggagaaa gagcataaca aagcgtttta ccagttgtgc
     8461 tgccatatgg agccgcagta ccaccagctt gagttcgaca cccggctgtg gctaacgcag
     8521 ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg cgttatcttt tctttcttta
     8581 gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg accatgcagc cccgccttct
     8641 tctccgtatt tgtttttctc gccggactct aaagattacc tgcttactgt tacttgtggc
     8701 aggagcgacc atttttatcg ccgatcgcgt aatggtcaac gccagcaagc agctgacctg
     8761 gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta ttgctggggg cgaggccggg
     8821 taatcgctac tttacccgac gtattgatac cgcagcagcg ttgtaccacg tcggaaaagt
     8881 gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat tacgatgaag cctcaggtat
     8941 gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg atcttctgcg actatgccgg
     9001 attctcaacg cttgattcag tggtgcgtgc caaaaaggtg tttggggaaa accatatcac
     9061 cattatctcg caagaatttc ataaccaacg cgccatctgg ctggcaaaac agtacggcat
     9121 cgacgctatc gggtttaacg cgccagacct taatatgaag cacggttttt atactcaact
     9181 gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc aaaatcctcc atcgccagcc
     9241 gaaatatctc gggccatcgg tcatgattgg gccgtttagt gagcatggct gtccggcgaa
     9301 agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc gtgctacatt gacgacagaa
     9361 tccctttatg gagtatccac gcgttatgat acgtttcgct gtgattggta cgaactggat
     9421 cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa tacaagttaa ccgccgtata
     9481 ttcccgcagc cttgaacagg cccagcactt cgccaatgat ttttctgtcg agcatctgtt
     9541 tacctcgctg gaagcgatgg cggaaagcga tgccattgac gcggtgtata ttgccagccc
     9601 gaatgccctg cacttttccc agacacaact tttccttagc cataaaattc atgtgatttg
     9661 cgagaaacca ctggcgtcga atctggcgga agtggatgcc gccattgcct gtgcgcggga
     9721 aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc ctgccgaact ttcatttgct
     9781 gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc tttttcaact attgccagta
     9841 ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc aacaccttta atccggcatt
     9901 ctctaacggt tcaattatgg atatcggctt ttactgcctg gcgtcggcgg tggcattatt
     9961 cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg gcaagcggcg tagatgccca
    10021 cggcgtggtg gtgatggatt acggtgattt cagcgttacg ttgcagcact caaaggtcag
    10081 tgattctgtc ctggaaagcg agattcaggg cgaagcggga tcgctggtga ttgaaaaact
    10141 gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc cagatgcagg atctcaccca
    10201 gccgcagcat attaatacca tgctctacga agcagagctg tttgctaccc tggtggatga
    10261 gcatctggtg gatcatccag ggctggcggt cagtcgcatc accgccaaac tgctgactga
    10321 gatccgccgc cagactgggg tgatttttcc ggcagatagc gtaaaactat aattgccaaa
    10381 gtaaaacagt gtaaaaggta tgtaacagac cattgactgg ctgaatggtc tgtcatactt
    10441 tgttacctgc aaaggggagt aacttcattg ccggtcgatc gtcattacga tgtgtgaaaa
    10501 accacatccg gtcaccgggc aacccgaaag gaatacgcag acgtattcct tttttgttgt
    10561 aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa gcagcggctg acgtcttccg
    10621 acgttggccg tttttttatg tgtaaggaac ttctatgaat actgtcggca cgccgttgct
    10681 atggggcgga ttcgctgttg ttgtcgccat tatgctggct atcgacctgt tgttgcaggg
    10741 gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg gcctggtcgc tggtctgggt
    10801 gacgctgtcg ttactgttta acgccgcttt ctggtggtat ctggtgcaaa ctgaaggtcg
    10861 cgccgttgcc gatccacagg cactggcctt tctcacgggt tatctgattg agaaatcgct
    10921 ggcggtcgat aacgtctttg tctggctgat gttgttcagc tatttctctg ttccggcggc
    10981 attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg attgttctgc gtaccatcat
    11041 gatcttcact ggcagctggc taatttcgca gttcgactgg atcctgtata tcttcggtgc
    11101 cttcctgctg tttaccggcg tgaagatggc gctcgcccat gaagatgaat caggcattgg
    11161 cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc atgaccgaca ccatcgacaa
    11221 cgagcatttc tttgtgcgta agaatggatt gctgtacgcc acaccgctga tgctggtact
    11281 gattctggtg gaattgagcg acgtgatttt cgccgtggat agcattccgg ctatcttcgc
    11341 cgtgaccact gacccgttca ttgtgctgac ctcaaacctg tttgcgatcc tcggcctgcg
    11401 tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc tcgatgctca aatatggcct
    11461 ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt gtcgacttct accatattcc
    11521 aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg atgacgttta ttatcaacgc
    11581 ctgggtgaat tatcggcatg ataagcagcg gggagaatga ttcgctgtat attaagccat
    11641 tcctagagat aactcat
//
LOCUS       DAZOYL010000112        11657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZOYL010000112 DAZOYL010000000
VERSION     DAZOYL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.112"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            266..937
                     /locus_tag="ACHUOY_002211"
     CDS             266..937
                     /locus_tag="ACHUOY_002211"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR0682980.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            947..2143
                     /locus_tag="ACHUOY_002212"
                     /pseudo
     CDS             947..2143
                     /locus_tag="ACHUOY_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2143..2703
                     /locus_tag="ACHUOY_002213"
     CDS             2143..2703
                     /locus_tag="ACHUOY_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0682981.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2705..3478
                     /locus_tag="ACHUOY_002214"
     CDS             2705..3478
                     /locus_tag="ACHUOY_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR0682982.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3666..3941
                     /gene="frmR"
                     /locus_tag="ACHUOY_002215"
     CDS             3666..3941
                     /gene="frmR"
                     /locus_tag="ACHUOY_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR0682983.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3976..5085
                     /gene="frmA"
                     /locus_tag="ACHUOY_002216"
     CDS             3976..5085
                     /gene="frmA"
                     /locus_tag="ACHUOY_002216"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR0682984.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5178..6011
                     /gene="fghA"
                     /locus_tag="ACHUOY_002217"
     CDS             5178..6011
                     /gene="fghA"
                     /locus_tag="ACHUOY_002217"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0682985.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6237..6776)
                     /locus_tag="ACHUOY_002218"
     CDS             complement(6237..6776)
                     /locus_tag="ACHUOY_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR0682986.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6878..8089)
                     /gene="mhpT"
                     /locus_tag="ACHUOY_002219"
     CDS             complement(6878..8089)
                     /gene="mhpT"
                     /locus_tag="ACHUOY_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR0682987.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8376..9389)
                     /gene="mhpE"
                     /locus_tag="ACHUOY_002220"
     CDS             complement(8376..9389)
                     /gene="mhpE"
                     /locus_tag="ACHUOY_002220"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR0682988.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9386..10336)
                     /gene="mhpF"
                     /locus_tag="ACHUOY_002221"
     CDS             complement(9386..10336)
                     /gene="mhpF"
                     /locus_tag="ACHUOY_002221"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR0682989.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10333..11142)
                     /gene="mhpD"
                     /locus_tag="ACHUOY_002222"
     CDS             complement(10333..11142)
                     /gene="mhpD"
                     /locus_tag="ACHUOY_002222"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR0682990.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11152..>11657)
                     /gene="mhpC"
                     /locus_tag="ACHUOY_002223"
     CDS             complement(11152..>11657)
                     /gene="mhpC"
                     /locus_tag="ACHUOY_002223"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR0682991.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2831 a         3136 c         2890 g         2800 t
ORIGIN      
        1 ttttaaatgc aattaattga caaactaaat cacatctaaa tacaatgtat aaaacaaaat
       61 aactatacat accaaacata caaacactta acatatgaat tgcataaaaa cattacccag
      121 tcgaacctcc gggtctgaaa gcccttgctt ttcagttatt agcaacattt aacatttata
      181 aattaacatc tggctgcgcc agagattacg tctctgagtg catcgttgtg cttgtagtat
      241 aataaataaa atggagtgtc attaagtgga taaggtttta gattcagccc tcctttcttc
      301 ggcaaataaa agaaagggta tccttgctat tggcgcacat cctgacgata tagaattagg
      361 ctgtggcgca tcgcttgctc gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat
      421 gactaccggc aactctggta cagatggaat aatcgatcgc catgaagaat cgcgcaacgc
      481 cctaaagata ttagggtgca accaaactat tcatcttaat tttgctgaca cccgcgctca
      541 tttacagctc aatgatatga tttccgccct ggaagacatc attaaaaatc aaattccttc
      601 tgatgttgaa atcatgcggg tatataccat gcatgatgcc gaccgccatc aggatcatct
      661 cgctgtttat caagcttcaa tggttgcctg ccgcactatt ccacaaattc tcggctacga
      721 aaccccaagt acctggcttt catttatgcc tcaggttttt gaatccgtta aagaagaata
      781 tttcacagtc aaacttgctg cattaaaaaa acataaaagc caggaacgac gtgattatat
      841 gcgtcatgat cgcctgcgtg cagttgcaca atttcgcggg caacaggtca atagcgatct
      901 gggtgaaggc tttgtaattc ataaaatgat tctttgagca aattgcatga aaacctggat
      961 atttatctgt atgtccatag caatgttgct atggttttta agtacgctaa gacgtaaacc
     1021 cagtcaaaag aaaggctgta ttgacgccat tatacctgcg tataacgaag gcccgtgtct
     1081 ggcgcagtca ctggataatc tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa
     1141 cgacggctcc acggacaata ccgaagcggt catggcggaa gtcaaacgca aatggggcga
     1201 ccgctttgtt gccgtcacgt aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct
     1261 caattatgcc acctgcgacc aggttttttt aagtgatgcc gacacctatg ttccgcccga
     1321 tcaagacgga atgggctata tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg
     1381 cattccctct actgcgttga aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa
     1441 gttgccgatg attgttatga agcgcacgct acagcagctc ctgggcggcg caccgtttat
     1501 tatcagcggt gcctgcggaa tgttccgtac tgatgtattg cgtaagttcg gtttctcgga
     1561 tcgtactaaa gtcgaagacc ttgatctcac ctggacatta gtggcaaacg gctaccgtat
     1621 tcggcaggcg aatcgctgca tcgtataccc acaggaatgc aacagtccgc gtgaggagtg
     1681 gcgtcgctgg cggcgttgga ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct
     1741 tttatttagc cgcttcggta tcttcagtat atttcctatg ctgttggttg tgatttatgg
     1801 cgttgggatt tatctcacta cctggtttaa tgaattcatc accaccgggc cgcatggtgt
     1861 ggtgttggca atgtttccgc ttatctggat cggcgtagtt tgtgttattg gtgcttttag
     1921 cgcctggttt catcgttgct ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt
     1981 attattagct tatgccatct ggattatcta tggacttatt gcctttttta ctggacgcga
     2041 acctcagcgc gacaaaccca cccgctattc cgcactggtg gaagcgtcaa ccgcttattc
     2101 ccaaccttct gtcacaggaa ctgaaaaact atctgaagct taatgttaaa gagaaaaatt
     2161 attcagattg cagaccaggt aattattgat gaaactgcag gtgaagttgt tatcggcgcg
     2221 aatacccgta tttgtcatgg tgccgttatt cagggtccgg tagtgattgg cgcaaactgc
     2281 ctgataggta attatgcgtt tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt
     2341 ggttttgcca ccgaaattaa aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa
     2401 tgttatattg ccgactcggt agttgcaaac caggcatatt tgggcgcaca agtacgtacc
     2461 agtaatcatc gtctggatga acagcctgtg tctgttcgaa ctccagaggg aattatcgct
     2521 accggatgcg ataaattagg ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt
     2581 attattttgc ctgggcgaat tatttccccg aacacgcaac ttggcccgcg cgtgattgta
     2641 gaacgtaatt tacctaccgg aacttattca ctccgacaag aacttatccg tacaggagat
     2701 taaaatgata aaacggacgt tattagcggc ggccattttt agcgcattgc ccgcttatgc
     2761 cgggttaact tccattaccg cgggctacga ttttaccgat tattctggcg atcatggcaa
     2821 ccgtaattta gcgtatgctg aactggtggc gaaagttgaa aacgcaacgc tgctttttaa
     2881 tctttcccag gggcgtcgtg attatgaaac tgaacatttc aatgccactc gcggtcaggg
     2941 tgcggtctgg tataaatgga ataactggct gacaacccga acgggtattg cctttgcgga
     3001 taatacgccg gtctttgccc gccaggattt tcgtcaggat attaacctgg ccctgttgcc
     3061 aaaaacgctt ttcacgaccg gttatcgcta cactaaatat tacgatgatg tcgaagtcga
     3121 tgcctggcaa ggcggcgtat cactctatac tggcccggtg atcaccagct accgctatac
     3181 ccattatgac tccagcgatg caggtggtag ttatagcaat atgatttccg tgcgtctgaa
     3241 tgacccgcgc ggcactggtt atacgcaact atggctaagc cgcggaacag gcgcttacac
     3301 ctatgactgg acgccagaaa cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat
     3361 tcaaccgctg actgagcaac ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac
     3421 cccaaccgat gattataacg gtctgcaact tgcagcccat ctgacctgga aattctgatt
     3481 gcttctgccg cccgctatcc ggggcggcct tccctgccga ttagccccac tccctttcct
     3541 cgttgttttc cgaccacatt caccggataa attttattct ccagcgttat atactatagg
     3601 gaggtatgca ttgacatata gcatacctcc ctatagtata ttgcgtgcag atgatgaggt
     3661 gcgaaatgcc cagtactccg gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc
     3721 gggggcagat tgatgctctg gaacggtcgc tggagggtga tgccgaatgc cgtgccatac
     3781 tccaacagat cgctgccgtt cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa
     3841 gccatatccg ggaaacgttt gaccgaaatg actgctacag ccgcgaagtc agccaatccg
     3901 ttgacgacac tattgaactg gttcgagcct atcttaaata gctgaatcta ttaccatatt
     3961 gaggaagagc gagagatgaa atcacgtgct gccgttgcat ttgctcccgg taaaccgctg
     4021 gaaatcgttg aaattgacgt tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc
     4081 cataccggcg tttgccatac cgacgcattt accctctccg gggatgaccc ggaaggtgta
     4141 ttcccggtgg ttctcggtca cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta
     4201 accagcgtca aacctggcga ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt
     4261 gagttctgtc gttctggcaa aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa
     4321 ggcttgatgc cagacggcac cacccgtttt tcttacaacg ggcagccgct ttatcactac
     4381 atggggtgct ctacattcag tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt
     4441 aatccagaag caaaccatga acacgtctgc ctgctgggct gtggcgtgac caccggtatt
     4501 ggcgcggtgc acaacacagc taaagtccag ccaggtgatt ctgttgccgt gtttggtctt
     4561 ggcgcgattg gtctggcagt ggttcagggc gcgcgtcagg cgaaagcggg acggattatc
     4621 gctatcgata ccaacccgaa gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc
     4681 attaacccga atgactacga caaaccgatt aaagatgtcc tgctggatat caacaaatgg
     4741 ggtatcgacc atacctttga atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa
     4801 agtgcgcacc gcggctgggg tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa
     4861 atctccaccc gtccattcca gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc
     4921 ggcgtgaaag gtcgttccca gttaccgggt atggttgaag atgcgatgaa aggtgatatc
     4981 gatctggaac cgtttgtcac gcataccatg agccttgatg aaattaatga cgccttcgac
     5041 ctgatgcatg aaggcaaatc cattcgaacc gtaattcgtt actgatttcc cgcaggtata
     5101 ccccgtccac ttcagacggg gttcttaata ctctccccgg gcagccgtcc gggggattaa
     5161 ccgtgagata atgactgatg gaactcattg aaaaacatgc cagctttggc ggctggcaaa
     5221 atgtgtatcg gcattattcc caatcactga aatgtgaaat gaatgtcggc gtctatctcc
     5281 caccaaaagc cgcgaatgaa aaattgccgg tgctgtactg gctttcaggc ctgacctgca
     5341 acgagcagaa tttcattact aaatcgggga tgcagcgtta cgcggctgag cacaacatta
     5401 ttgttgttgc gccggacacc agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg
     5461 atctcgggca aggtgccggg ttttacctga acgcaacgca agcgccgtgg aatgaacatt
     5521 acaaaatgta tgactatatc cgcaacgagc tgccggattt agtgatgcaa cattttccgg
     5581 caacgaccag aaagtctatc tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg
     5641 cgttacgtaa cccagatgaa tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat
     5701 cgcaagtacc gtggggacag caagcctttg ccgcatatct tggtgaaaat aaagatgcct
     5761 ggttggatta cgatccggtg agtcttattt cacaaggtca acgcgttgcg gaaatcatgg
     5821 ttgatcaggg gttgagtgat gatttttacg cggaacagct gcggactcca aatcttgaaa
     5881 agatctgcca ggagatgaat atcaagacgt taatccgtta tcacgagggt tacgaccaca
     5941 gctattattt tgtctccagt tttattggcg agcatattgc ctaccacgcc aataaactga
     6001 atatgcgttg ataatagtgc acgactgccg gatgcggcgt gaacgcctta tccggcctac
     6061 acttcgcccg taaaccgtag gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc
     6121 acgactgccg gatgcggcgt gaacgcctta tccggtctac acttcgccca taaaccgtag
     6181 gcctgataag acgcgcatag cgtcgcatca ggcatctgcg cacgacttat ctatgtttac
     6241 cacatcaaat catcaggcac tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg
     6301 gcactgagcg cgctgtgcaa cacaatactg ctggcatcac gctgagcaat tttatcggca
     6361 acactggcgg gaataatggt gtattcacct tcgctattgt tatcgaccaa caagcgggca
     6421 atcgccagac gaccattaat caactgcgcc tgtgtgagct tatcgacaaa aatcttctta
     6481 atcagattgc cgtcagtgaa gttaaaacca atatcgccat tggcaatggt gattcggttc
     6541 atttcaatga gctgcttcac ctgggcttta tattctttcg ccaacgccgc ttgtttttgc
     6601 tgttcgctaa gctgtttatc acgctcaagc tgtgcctttt tattttcttc taccgcagcc
     6661 cgagcttcac gcgcctgaac gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt
     6721 ttgctggtga ctaatcctgc tttgagcaac tgctcttgta aggtaagttt tgccatcttt
     6781 gtttccaaac ccgctgaata atttttagcg agtatacctg taaacgccgt gggtgtattg
     6841 ggggcaagtc cctttcgccc ctgcttaata aggcgcgtca ggcatcggca cacggctgca
     6901 ttcttgatct ccgactcatc aaaataaaca ccgccaaccc agcaacaaga atacccggtg
     6961 cagaagcggc cattacgccg accgtgccag tgcctaatgc cagcattttc ccggccagta
     7021 acggaccgct catagccccc agacgcccta ccgccacggc tgttcccaca cctgttgcgc
     7081 ggatctgcga actgtaaaac aacagtgcca gggcatacaa aacgctttgc ccacctgtcg
     7141 caaacaaccc cgcgacaaat cccgccagca acataccgtt aaatgacgac accgttccaa
     7201 gcgccagcag cgaagctaac atgccgctat aaatcagtag cgacatggtt actggacgca
     7261 gcttatccat caatgcgccc aacattaacg tcccgcttgc cgcccccatt tgcagggcaa
     7321 acatcacccc tgccgcctgc gatggctgga atccttgctc caccaaaagt agcggtagcc
     7381 agttgatcaa catataaacc accagcagag tgaagaaata acacaaccac agcagcagcg
     7441 tcgcggttgc cgtttctggc gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc
     7501 cagcgaaaac cgctgactcc ggcagccagc gcattaatag cggcaccaga atcaacggca
     7561 ccacaccacc tacccaaaac accgtttgcc atgctaagtt tgcccccgcg aaacccagtg
     7621 tcgccgccag cgccgcgcca atgggaacac cgcaatacat caggctcact gccgtcccac
     7681 gaaaacgtgg acccgcggct tcagatgtca gggcgataag attcggcaac gccgccccca
     7741 gcccgacacc ggtcatcagc cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg
     7801 ttgccagtga gaacaaacca aacagcgcaa ctgagccaat caaaatacgc ttacgaccat
     7861 aacggtctgc cagcatcccg ccaaccaacg cgccgggtag caaaccgagt attccggcgc
     7921 taaatatcca gcccatttgc attttatcga gtgcgaaagc ctgggcgatg ccacccgccg
     7981 caatgccagc cgcctgaaga tccagccctt ccatcagagc gaccaaaaaa caaagcccga
     8041 tggtcagcat caggcgggat gaagatgatg aaggggtacg agtcgacata agatgaacct
     8101 cattgcgagt gcaggtgaat cgcgactgat acattgccag ccgcgtaatc agaactattt
     8161 aatgactgcc tacgggcgaa atgtagcgaa atgtaggccg gataaggcgt tcacgccgca
     8221 tccggcagtc gtgcgcagat gcctgatgcg acgctaacgc gtcttatcag acctacaaat
     8281 tctcacgcca tccgtaggcc ggacaaggcg ttcacgccgc atccggctgt cgtgctggga
     8341 tgggcataca aaatgcacgt tatcggcagt catcattatt tgttgttgcg cagatccagc
     8401 gccacgtcga cgatcatatc ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg
     8461 agaatatcca ccgcacttaa gccataacgc gccgccgccg tttcacagtg acgcaggaag
     8521 ctcgagtaaa caccagcgta tcccagcgcc agcgtttcgc gatcgactcg taccggacga
     8581 tcctgcaacg gacgcaccag gtcgtcggcg gcatccatta acgcatagag atcggtccca
     8641 tgctgccagc ccagtttatc cgcggcggca ataaacactt ccagcggtgc gttacctgcg
     8701 cccgcgccca ttcccgcgag gctggcgtcg attcggtcgc agccctcttc caccgccgcg
     8761 atagagttcg ccacgccaag actcaggtta tggtgagcgt gcatgccggt ttgcgtttct
     8821 ggtttcaaca ctgctttcag ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca
     8881 ccgccagaat ccaccacata aatacaggtc gcaccgtagc cttccatcag ctttgcctgc
     8941 ttggcgagat tctccggcgt ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt
     9001 ccgagctcgc gggcatactg aatatgctgg gcggaaacat cagcttcggt acagtgcgtt
     9061 gccacacgaa ccacccgcgc gccagcctgc caggcatttt tcagatcgtg aatagtgccg
     9121 attcctggca gcaacaacgt cgcgattttg gcgtgcctca ccacatccgc cgccgcttca
     9181 atccattcaa ggtcgctatg tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg
     9241 tcgccgtggg ccacttcaat cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg
     9301 cgaacgtttt ccagcgaata ctgatgacga atggcgtgca taccgtcacg caatgtgacg
     9361 tccgagatat aaagtttttt accgttcatg ccgcttctcc tgccttgcgc gccagtgact
     9421 gggccatttt ttccgctgtc gccagcgcac tggaagtcat aatatcgagg ttgcccgcat
     9481 aggcaggcag ataatgcgct gcgccttcga cttccagcca gaccgctgtt ttcagtccgg
     9541 agaattgccc cacgcccggt aaattgaccg gtttatcctg cgggataact tcaaactgca
     9601 cgcgctgttt caggcgataa cccggtacgt aagcctgcac cgcctcagcc atttcattga
     9661 ttgaggcttc gatatcatct tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca
     9721 tcatcaacgg tggctctgct gggttaagca caataatcgc cttccctttt gccgcgccgc
     9781 ccaccacttc aatggctcgg gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc
     9841 caggtccggc agatttactg gcgatagaag cgataatttc ggcgtaatga acacgcgcca
     9901 cgcgtgaaac tgccgccacc attggaatgg tggcctggcc gccgcaggtg accatgttga
     9961 cgttcagttg atcgacgttc gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg
    10021 cagcaggcgt caggtcaatt aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt
    10081 ttttcacatg cgcgcccgcg ctggtcgcat caaatacaat gtcgatatca gcaaattcag
    10141 gcatgttcat cagcccgatc accccttcat gggtggtggc gacgcccata cgtctggcac
    10201 gcgccagacc gtcggactga ggatcaatgc cgaccatcac cgccatctcc agatgctgac
    10261 cgtgacgcaa aattttaatc atcagatcgg tgccaatgtt gccagaaccg ataatggcga
    10321 ctttacgctt actcatgaca gacttccttt tggggctgcg cttgaaaatg tcgcagcaac
    10381 tgaacctatg ccttcaatat gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg
    10441 gcctaatgcc ccggtaagaa tgatatctcc ggcgcgcagc ggttcaccca gactggccat
    10501 tttgcgtgcc agccagacgg cggcattaag cggatgtccc aggcattcgc tgccgcgccc
    10561 gctggaaacc tcttcgttat tacgcgtcat cttcatggcg cagtttttca ggtctaaccc
    10621 cgccggacgt tgcgccggac cgccgatgac atacaccccg caggaagcgt tatctgccac
    10681 ggtatcgaca aactgaatcg accagtcgcg aatgcggctc cccaccactt ccagcgccgg
    10741 aagtacccat tcaatggcgt tatacagttc gtcgaaggtg atatcagttg cgggtaaatc
    10801 gcggttcaac accagtgcga tctccgcttc aatgcggggt tgcagaacac gggaaaaagg
    10861 aatgatttcg ttatcgccat aacacatgtc ggcaaataac gtcccaaaat ccggttgatc
    10921 aacgcccagt tgttgttgca ctttcggatg cgtcaggccc actttacgcc ctaccacgcg
    10981 acgcccctgc acaacgtcat attgcacatt tatgtgctga atggcgtagg ccgcttcagc
    11041 gtgatcgata ccaatcagat cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg
    11101 gcgtaaatcc gccgccagtt gctcaagagt atgcttcgtc atgaccattc cttaagggcg
    11161 tgcgaggaaa ttcagcacca attgattgaa agcgtcggca tgttcccact gcgcccagtg
    11221 accacagtcg cggaagatat gcagttcaga accggcaatg ccggacagca gacgcagacc
    11281 cgcatccatc ggcacaaagc ggtcgttgcg cccccagaca atcagggttt gcgctttgat
    11341 ttccgccaga cgtgggccaa aatccgggaa ctgtttcgga ttagcttcca ggctcttaac
    11401 gaagttttcc aggtgatcgc ggcgcgacag catattattc aggcgcgctt caaacagggc
    11461 gtcggtcaaa tcgctggtat cgaaaacgaa gatatccatc atcagcttca ggttttcgat
    11521 agtcggctga cgataaagct gattcagtcg cttaatacct tcggttggca tcggcgtaaa
    11581 caaactcatg ccgcccgtac cgccgcccat cagcaccagt ttgccgacgc gctccggcca
    11641 gttaagggtg aacgcca
//
LOCUS       DAZOYL010000113        11612 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZOYL010000113 DAZOYL010000000
VERSION     DAZOYL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.113"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(71..655)
                     /gene="pth"
                     /locus_tag="ACHUOY_002224"
     CDS             complement(71..655)
                     /gene="pth"
                     /locus_tag="ACHUOY_002224"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR0682992.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            932..1210
                     /gene="ychH"
                     /locus_tag="ACHUOY_002225"
     CDS             932..1210
                     /gene="ychH"
                     /locus_tag="ACHUOY_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR0682993.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(1265..2944)
                     /gene="dauA"
                     /locus_tag="ACHUOY_002226"
     CDS             complement(1265..2944)
                     /gene="dauA"
                     /locus_tag="ACHUOY_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR0682994.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(3068..4015)
                     /gene="prs"
                     /locus_tag="ACHUOY_002227"
     CDS             complement(3068..4015)
                     /gene="prs"
                     /locus_tag="ACHUOY_002227"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR0682995.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(4166..5017)
                     /gene="ispE"
                     /locus_tag="ACHUOY_002228"
     CDS             complement(4166..5017)
                     /gene="ispE"
                     /locus_tag="ACHUOY_002228"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR0682996.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(5017..5640)
                     /gene="lolB"
                     /locus_tag="ACHUOY_002229"
     CDS             complement(5017..5640)
                     /gene="lolB"
                     /locus_tag="ACHUOY_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR0682997.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            5854..7110
                     /gene="hemA"
                     /locus_tag="ACHUOY_002230"
     CDS             5854..7110
                     /gene="hemA"
                     /locus_tag="ACHUOY_002230"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR0682998.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7152..8234
                     /gene="prfA"
                     /locus_tag="ACHUOY_002231"
     CDS             7152..8234
                     /gene="prfA"
                     /locus_tag="ACHUOY_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR0682999.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            8234..9067
                     /gene="prmC"
                     /locus_tag="ACHUOY_002232"
     CDS             8234..9067
                     /gene="prmC"
                     /locus_tag="ACHUOY_002232"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0683000.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            9064..9456
                     /gene="sirB2"
                     /locus_tag="ACHUOY_002233"
     CDS             9064..9456
                     /gene="sirB2"
                     /locus_tag="ACHUOY_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR0683001.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            9460..10268
                     /gene="sirB1"
                     /locus_tag="ACHUOY_002234"
                     /pseudo
     CDS             9460..10268
                     /gene="sirB1"
                     /locus_tag="ACHUOY_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
     gene            10304..11158
                     /gene="kdsA"
                     /locus_tag="ACHUOY_002235"
     CDS             10304..11158
                     /gene="kdsA"
                     /locus_tag="ACHUOY_002235"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR0683002.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(11307..11414)
                     /locus_tag="ACHUOY_002236"
     CDS             complement(11307..11414)
                     /locus_tag="ACHUOY_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0683003.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         2901 a         2951 c         3054 g         2706 t
ORIGIN      
        1 cagattgcag aaatggaaat aactttgcct attatacacg gcactcggca aaaatgccgc
       61 agacaacgac ttattgcgct ttaaaggcgt gcaatcggtt cgttgctttg gtcaagccat
      121 ctgtaaacca catttcagta caacgcgccg cttcgtcgat ggcttcatca attaacttct
      181 gttcactaac aggcggtttg cctaacacaa aaccgacaac tttattttta tcgccaggat
      241 gaccgattcc gatgcgtaaa cggtgaaagt tagggttatt acccaattta ctgatgatgt
      301 ctttcagtcc attgtgacca ccatggccac cgcccaattt aaatttggcg acgccaggag
      361 gcagatccag ttcgtcgtgg gccaccagaa tttcgtccgg attaatgcgg aaaaaactgg
      421 ccatcgccgc aacggctttg ccgctgagat tcataaatgt agtcgggact aacaggcgga
      481 catcttcgcc tccaagagtg actcgcgaag tataaccaaa gaatttagcc tcttcgcgca
      541 gcggagcgcg caaacgctct gccagtaagt caacgaacca ggcaccagca ttatgtcgcg
      601 ttgcggcgta ttcagcaccg gggttcgcca ggccgacaat caatttaatc gtcacgtttt
      661 ttgtcctgag tgtgtacata actggcgcgt agtttactgg ttgcggcccc gcttgacaaa
      721 aaactgcgta tcaaatgcag ataacgtaat aattgcctga gtggactatt agaaagtcaa
      781 ggtgttcagg cgtttatttg taaagttttg ttgaaataag ggttgtaatt gtgatcacgc
      841 ccgcacataa cccacagggt gttgtctata ctttacacat aaggaagagg ggtattccct
      901 gttacaaccc agaaagttcc ggaggtgaca tatgaaacgc aaaaacgctt cgttactcgg
      961 taacgtgctc atggggttgg ggctggtggt aatggtggtc ggcgtggggt attcaatcct
     1021 caaccagtta ccacagttta atatgcccca gtatttcgca catggtgcag tgctaagtat
     1081 tttcgtcggt gccattctct ggctggcggg tgcccgtgtt ggcgggcatg aacaggtgtg
     1141 cgaccgttac tggtgggttc gtcactatga caaacgttgc cgccgtagcg ataatcgccg
     1201 tcatagctaa caaataatgc agtttgctga ccagtcagtt tgcgctgact ggtcaatctc
     1261 gtacttataa atccgccatc gccgcgcgac gattcgggaa gaacgcgagg cgtcccggga
     1321 tcggttgaat gcctgcgcga gccatagtgc gcagtggctg gaattccacg ttgcacacgc
     1381 gcagttcaca tccttcgggc agacgcttca caaaacgctg gaacgcatca agaccaccag
     1441 catcaagtac cggaacggca tcccacttca gaatcacaat ccgtttgcct tcaagacgtg
     1501 actccaggtc cgtgaacaag ccttcagcag cagcaaaaaa cagcgggcca ataacgcgca
     1561 gtaccagaac atcgtctgga acatctacga ctaccggtgc caggcgagtc atacgtgcga
     1621 tacgacgcat aaacagcagc gatgccagca cgatccccac gctgatggca ataaccatat
     1681 caaacagcac ggtcagcgac atgcacagca gcatgacaat gatgtcatct ttcggtgcat
     1741 gacgcagcaa gtcgaccact ttatgcgctt cactcatgtt ccacgccacc atcaacagca
     1801 gggctgccat agcggaaagc ggcagccagg agagcagcgg tgccagtacc agcagggcaa
     1861 gaataaccag aatagagtgg atcaccgccg agatagggga agttgccccg gcacggacgt
     1921 tagcggcaga acgcgcgatg gcagctgtag cggtaatacc accaaagaac ggagcgataa
     1981 tattccccag tccctgtcca accagttcgc tgttcgcctt gtgtttcgtc ccggtcatac
     2041 catccagtac cacggcgcag agcagagatt cgattgcgcc gagcattgcc attgagaatg
     2101 ccgcaggcag cagtgtgcga atagaatccc aggttagcgt gaattctgaa ttaggcagat
     2161 cccacggcag caccagttgc ggcagcagtt gcggaatacc gttaccctga gaaccatcgg
     2221 ccaggacgta gtggaattgc gaaccgatgg tagcaacatg tccgccgagc aggttaacaa
     2281 tccccatcac cgcgcaacca gccagcaagg ccggaaggtg accgggtaaa cgaatgccca
     2341 gacgcggcca aaaaacaaga atacctagcg tcacaatgcc aatggcagca tcacccacat
     2401 taatggtcgg cagcgccata aataatgcgc cgactttttg tagataatgt tccgggacat
     2461 gggccatttg cagaccgaga aaatctttaa tctgcatggt accgatggtg atcccgatac
     2521 ccgaggtgaa acctaaggtg acggaaaccg gaatatactc aatcaggcga ccaaagcgtg
     2581 ccagacccat cagaatcaaa aagatccccg acagcaaggt cgcaaccagc agtcctgcca
     2641 gtccaaactg ttgcgacacg ggatagagaa ttaccacaaa tgccgcagtc ggaccggaaa
     2701 cgctaaagcg tgacccaccc gtcagagcaa tgacaatccc cgcaacagct gcggtatata
     2761 aaccgtactg gggtgccaca ccactaccaa tagccaacgc catcgccagc gggatagcaa
     2821 taatcccgac ggttatcccg gcaatcaggt cacgggtaaa ccgtgcggca gtatattttt
     2881 ctttccagca agcgtcgatc agagcgcgga aaggcatcac atgtgaggaa aatattttgt
     2941 tcacaataat gtttcatccg tgagcgcatc atctgtcaac taaatggcag gtgaaggagg
     3001 cataggtcat acaaatggat attacagaca aaaaaacccg ccgcagcggg tctttgagcg
     3061 ggttcgatta gtgttcgaac atggcagaga tcgattcttc gttgctgata cgacgaatcg
     3121 cttcggccag catacctgac agggtcagag tacgcacgtt cggcagtgat ttgatttcat
     3181 cgctcagcgg aatggtatcg cagacaacga cttcatcaat tacagagtta cgcaggttgt
     3241 tcgccgcgtt gccagagaag atcgggtgag tcgcgtacgc aaatacacgt ttagcaccac
     3301 gttctttcag agcttcagca gctttacaca gcgtaccgcc agtgtcgatc atatcatcga
     3361 ccagtacgca gtcacgacct gcaacgtcac cgatgatatg catcacctgg gaaacgttcg
     3421 cacgcggacg acgtttgtcg atgattgcca tatcggtatc gttcagcagc ttagcgatag
     3481 cgcgggcacg cacaacgccg ccgatgtccg gagaaaccac aattgggtta tccagattca
     3541 gctgcagcat gtcttccagc aggatcgggc taccaaatac gttatcaacc ggaacgtcga
     3601 agaaaccctg aatctgttca gcgtgcagat ccactgtcag cacacggtca acaccgacgc
     3661 tggagaggaa gtctgcaacc actttcgcag tgattggtac acgagcggaa cggacgcgac
     3721 ggtcctggcg cgcatagcca aagtagggga taacagcggt gatacgacct gcggaagcac
     3781 gacgcagggc atcaaccata acgactaatt ccatcaggtt gtcgttagta ggggcacaag
     3841 tggactggat gatgaaaata tcaccaccgc gtacattttc gttgatttgt acactgactt
     3901 cgccgtcgct aaagcgacct acagcggcgt cgccgagtga agtgtacagg cggttggcaa
     3961 tacgttgtgc tagttccggg gtggcgttac cagcaaaaag cttcatatca ggcacgagaa
     4021 gaacctcagg catgcgtcca ttggtggaaa gaatctgccg aaaactgtgc gggccaggca
     4081 tgatcctttc caggcggtgt attaaagagc gcgatgcaac gtctggaaca aggtgacgtt
     4141 gtcaccgaaa ctcagcttgc ccggcttaaa gcatggctct gtgcaatggg gaaagattaa
     4201 cacctttcgc cacaaagcca ttgagccatt ccggggcttg ctctagcacc tggcgggctt
     4261 cagactctgt atcaaattca gcaaagacac aggcccctgt cccagtcagg cgcgacgggg
     4321 cgtattctaa cagccaggaa agcaccgcat caacctcgcg aaaacgtttt cttgcgataa
     4381 cctcgcaatc attgctgaat tcacatttta gcaacgtttc tattgacctt tttggcgtat
     4441 tgcgcgggag ttcaggatct ttaaaaatca ctggtgtcgg aatacttaca ccagggtgcg
     4501 ccaccagata ccacttctct ggcggatcca ccggcgttag tatttcacca acgccttcgg
     4561 caaacgcggc atgcccccga acaaagacag gaacatctgc gcccagcgtc agccccattt
     4621 ccgccagctc atccatgctt agcccgcatt gccagagatg atttaatgcc accaggaccg
     4681 tcgcggcatt ggatgaacca ccgccgagac cgccgcccat cggcaaacgc ttgtcaatgc
     4741 tgatattcgc accgcttccc gtcggaagac gcccgctgtc tgccgcagtt ttcatcaaca
     4801 accgcgctgc gcgaacgatc aggttatctt catgttccac gccttcaacg ggcgttaaca
     4861 gacgaatatc cccatcgtca cgaagctcaa tgctgatggt gtcgccgtaa tcaagaaact
     4921 gaaacagcgt ttgcagcgtg tggtaaccat ccgcacgctg accggtaatg tataaaaaca
     4981 gattaagttt tgccggagag ggccactgtg tccgcattat ttcactatcc agttatccat
     5041 ttttaacttg atgcgttgac caccgtcggt gagttccata ttggctggca tcgcaggttg
     5101 cgttttggtg tcataaccac cataaacaac cttccagttt ttgccattct ggctgtaggt
     5161 aatttcgctc aggcggtact ggtcgtccag tttgtagtcg gttgcatcac ccggtaaacc
     5221 taaaatccat tggcgcaagc tgttgagcgg aattggcatt ccggtcaatt tgccaatcat
     5281 ctcttcggcg tcatcggcgg tataacgctg acctttattg tcgactaact gcacgttacc
     5341 cggttgagca ttcagctcca gttccgtgct gcccaatggg ttagtgagca gcagacggta
     5401 gcgatcctgg ccggtttgct gccagaaaaa gcgggcgtac actttttgtt ggtcagaaat
     5461 ataagcgaac gcgccgcgag tctgatactg attaagattg cgcacgtctt gctgatgctg
     5521 acgccattgt ggcgaatccg ggcttttgcc aggacctttg ggcgtggtaa cggaacaggc
     5581 agtgagcaca agagcagcca gcggtagcag gcggataaga cgaaaatcgg gctggggcat
     5641 agtgatgaca agtccttgag atacgttgca gttataaccc ttaatgctag cgttaccgtc
     5701 cgctatcgtc tatgttcaag ttgtcttaat tgccagaatc taacggcttt cggcaattac
     5761 tccaaaaggg ggcgctctct tttattgatc ttacgcatcc tgtatgatgc aagcagacta
     5821 accatatcaa cgttggtatt atttcccgca gacatgaccc ttttagcact cggtatcaac
     5881 cataaaacgg cacctgtatc gctgcgagaa cgtgtatcgt tttcgccgga taagctcgat
     5941 caggcgcttg acagcctgct tgcgcagccg atggtgcagg gcggcgtggt gctgtcgacg
     6001 tgcaaccgca cggaacttta tcttagcgtt gaagagcagg ataacctgca agaggcgtta
     6061 atccgctggc tttgcgatta tcacaatctt aatgaagaag atctgcgtaa aagcctctac
     6121 tggcatcagg ataacgacgc ggttagccat ttaatgcgtg ttgccagcgg cctggattca
     6181 ctggttcttg gggagccgca gatcctcggt caggttaaaa aagcgtttgc cgattcgcaa
     6241 aaaggtcata tgaaggccag cgaactggaa cgcatgttcc agaaatcttt ctctgtagcg
     6301 aaacgcgttc gcactgaaac agatatcggt gccagtgctg tgtctgtcgc ttttgcggct
     6361 tgtacgctgg cgcggcagat ctttgaatcg ctctctacgg tcacagtgtt gctggtaggc
     6421 gcgggcgaaa ccatcgagct ggtagcgcgt catctgcgcg aacataaagt acagaagatg
     6481 attatcgcca accgcactcg cgaacgtgcc caaattctgg cagatgaagt tggcgcggaa
     6541 gtgattgccc tgagtgagat cgacgaacgt ctgcgcgaag ccgatatcat catcagttcc
     6601 accgccagcc cgttaccgat tatcgggaaa ggcatggtgg agcgcgcatt aaaaagtcgt
     6661 cgtaaccaac caatgctgtt ggtggatatt gccgttccgc gcgatgttga gccggaagtt
     6721 ggcaaactgg cgaatgctta tctttatagt gtggacgatc tgcaaagcat catttcgcac
     6781 aacctggcgc agcgtaaagc cgcagcggtt gaggcggaaa ctattgtcgc tcaggaaacc
     6841 agcgaattta tggcgtggct gcgagcacaa agcgccagcg aaaccattcg cgagtatcgc
     6901 agccaggcag agcacgttcg cgatgagtta accgccaaag cgttagcggc ccttgagcag
     6961 ggcggcgacg cgcaagccat tatgcaggat ctggcatgga aactgactaa ccgcttgatc
     7021 catgcgccaa cgaaatcact tcaacaggcc gcccgtgacg gggataacga acgcctgaat
     7081 attctgcgcg acagcctcgg gctggagtag cagtacatca ttttcttttt ttacagggtg
     7141 catttacgcc tatgaagcct tctatcgttg ccaaactgga agccctgcat gaacgccatg
     7201 aagaagttca ggcgttgctg ggtgacgcgc aaactatcgc cgaccaggaa cgttttcgcg
     7261 cattatcacg cgaatatgcg cagttaagtg atgtttcgcg ctgttttacc gactggcaac
     7321 aggttcagga agatatcgaa accgcacaga tgatgctcga tgatcctgaa atgcgtgaga
     7381 tggcgcagga tgaactgcgc gaagctaaag aaaaaagcga gcaactggaa cagcaattac
     7441 aggttctgtt actgccaaaa gatcctgatg acgaacgtaa cgccttcctc gaagtccgag
     7501 ccggaaccgg cggcgacgaa gcggcgctgt tcgcgggcga tctgttccgt atgtacagcc
     7561 gttatgccga agcccgccgc tggcgggtag aaatcatgag cgccagcgag ggtgaacatg
     7621 gtggttataa agagatcatc gccaaaatta gcggtgatgg tgtgtatggt cgtctgaaat
     7681 ttgaatccgg cggtcatcgc gtgcagcgtg ttcctgctac ggaatcgcag ggtcgtattc
     7741 atacttctgc ttgtaccgtt gcggtaatgc cagaactgcc tgacgcagaa ctgccggaca
     7801 tcaacccagc agatttacgc attgatactt tccgctcgtc aggggcgggt ggtcagcacg
     7861 ttaacaccac cgattcggca attcgtatta ctcacttgcc gaccgggatt gttgttgaat
     7921 gtcaggacga acgttcacaa cataaaaaca aagctaaagc actttctgta cttggtgctc
     7981 gtatccacgc tgcagaaatg gcaaaacggc aacaggccga agcgtctacc cgtcgtaacc
     8041 tgctggggag tggcgatcgc agcgaccgta accgtactta caacttcccg caggggcgcg
     8101 ttaccgatca ccgcatcaac ctgacgctct accgtctgga tgaagtgatg gaaggtaagc
     8161 tggatatgct gattgaaccg attatccagg aacatcaggc cgaccaactg gcggcgttgt
     8221 ccgagcagga ataatggaat atcaatactg gttacgtgaa gcaataagcc aacttcaggc
     8281 gagcgaaagc ccacgccgtg acgctgaaat cttattggag tatgtcactg gcagggggcg
     8341 gacttttatt ctggcctttg gtgaaacgca gctgactgac gaacaatgtc agcaacttga
     8401 tgcgctactg acacgtcgtc gcgatggtga acccattgct catttaaccg gggtgcgaga
     8461 attctggtcg ttgccgttat ttgtttcgcc agcgacctta attccgcgcc cggatacgga
     8521 gtgtctggtg gagcaggcac tggcgcggtt gcctgaacaa ccttgccgta ttctcgatct
     8581 cgggacgggt accggggcga ttgcgcttgc gctggctagc gagcgcccgg actgcgaaat
     8641 tatcgctgta gatcgtatgc ctgatgctgt ctccctggca caacgtaatg cccagcatct
     8701 ggcgatcaaa aatatccaca ttctgcaaag cgactggttt agcgcgctag ccgggcagca
     8761 gtttgcgatg attgtcagca atccgccgta tattgacgag caggacccac atcttcaaca
     8821 aggcgatgtc cgctttgagc cgctcactgc gctggttgcg gcagacagtg gaatggcaga
     8881 catcgtgcat atcatcgaac agtcgcgtaa cgcgctggta tccggcggct ttctgcttct
     8941 ggaacatggc tggcagcagg gcgaagcggt gcgacaagca tttatcctcg cggggtatca
     9001 tgacgtcgaa acctgccgtg actatggtga taacgagcgc gtaacgctcg gccgctatta
     9061 tcaatgacaa gtttttctac actgcttagt gttcatctta ttagtatcgc gctttctgtt
     9121 gggctattaa ccttacgttt ctggctacgt tatcagaagc atccacaggc atttgctcgc
     9181 tggacgcgca ttgtgccgcc ggttgtcgat acggtgttat tgttaagcgg cattgcgttg
     9241 atggctaaag cgcacatcct gccattttcc gggcaggcac agtggctgac tgaaaagctg
     9301 tttggagtta tcatttatat cgttttgggt tttattgcac tcgattatcg tcgtatgcac
     9361 agtcagcagg cgcgcattat tgccttcccg ctggcgttgg tggtgctgta catcatcatt
     9421 aaactcgcca ccacaaaagt accgttactg gggtaagtca tgagatcgtt agctgatttc
     9481 gaatttaata aagcgccatt gtgcgaaggc atgatcctgg cttgcgaagc aatccgccgc
     9541 gattttccct cgcaagatgt ttacgacgaa ctggagcgtc tcgttagtct ggcgaaggaa
     9601 gaaatcagcc agcttctgcc tttagaagag cagctggaaa aactgatcgc gctgttttac
     9661 ggcgactggg gatttaaagc ctcacgcggc gtttatcgtc tttccgatgc attatggctg
     9721 gaccaggtgt taaagaatcg acaggcagtg cggtatcatt aggtgcggtt ttattatggg
     9781 tcgcgaatcg tctcgatttg ccgctgctgc cggtgatttt ccctacgcag ctgatattgc
     9841 gcattgaatg tccggatggc gaaatttggc tgattaatcc ttttaacggt gaatcgttaa
     9901 gcgaacatat gctggacgta tggttaaagg gaaatatcag cccgtcggcg gaactgtttt
     9961 atgaagacct tgatgaagct gataacattg aggtaatccg caaattgttg gatacactca
    10021 aagcctcgtt gatggaagaa aatcagatgg agctggcgtt acgcaccagc gaagctttat
    10081 tacaattcaa cccggaagat ccctatgaaa ttcgcgatcg cgggttgatt tatgcgcaac
    10141 tggattgcga acacgttgcg ttgaacgatt taagttattt cgttgaacag tgtccggaag
    10201 acccgatcag cgaaatgatc cgtgcgcaaa taaataacat cgcgcataaa catattgtgc
    10261 tgcattaatt aatcgacatt ttactcaaga ttaaggcgat cctatgaaac aaaaagtggt
    10321 tagcattggc gacatcaacg tagcaaatga cctgccgttc gtactgtttg gtggtatgaa
    10381 cgtgctggaa tctcgcgatc tggcgatgcg catttgcgag cactacgtaa ccgtgaccca
    10441 gaaactgggt atcccttacg tgttcaaagc ctcttttgac aaagccaacc gctcctccat
    10501 ccactcttat cgtggaccgg gcctggaaga agggatgaaa atcttccagg agttgaagca
    10561 gacttttggc gtgaaaatta tcaccgacgt tcacgaacca agtcaggcac agcccgttgc
    10621 tgatgtcgtg gatgtgattc agttgccggc gtttcttgct cgccagactg acctggttga
    10681 agccatggcg aaaaccggtg cggtaattaa cgtcaagaaa ccacagtttg tcagcccggg
    10741 acagatgggt aatatcgttg ataaattcaa agaaggcggc aacgaaaaag tgattctttg
    10801 cgatcgcggt gctaacttcg gctatgacaa cctggttgtc gatatgctgg gcttcagcat
    10861 tatgaagaaa gtgtctggta actcgccggt gattttcgac gtgacccacg cactgcaatg
    10921 ccgcgatccg tttggcgcag cttccggtgg tcgtcgtgct caggtggctg agctggcacg
    10981 agccggtatg gcggtaggtc tggcggggct gtttattgaa gcgcatccgg atccggaaca
    11041 tgcgaaatgt gatggtccat ccgcgctgcc gctggctaaa ctggaaccgt tcctcaagca
    11101 gatgaaagcg attgatgatc tggtgaaagg tttcgaagaa ctggatacca gcaagtaatc
    11161 ttttttgctt gaaaaataaa gccttagcgt tctgcgttaa gacttttttc atgggtgcca
    11221 gatacaaaaa aggccgcagg ctgttacccc tgcggccggt ttcgggcgca tattgccatc
    11281 acggcagcct gacgcccgtt ttcaccttac ttccggttac gccaccagcc gacaatcgct
    11341 gcggtaataa ttcccgccag gatcggtgct gccaggtcgt gccagaaaat catggcaaac
    11401 tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca ttttgcggct attcttgaat
    11461 tgttctggtt caagattagc ccccgttctg ttgtcaggtt aaacctctca acgtgcgggg
    11521 gttttctctt tccagcaacc aatgccacca gggataaagc ccccgcaaca ttgcgcctca
    11581 ccggataacg ccggcttgat gtggatacta cg
//
LOCUS       DAZOYL010000114        11541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZOYL010000114 DAZOYL010000000
VERSION     DAZOYL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.114"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            459..1385
                     /locus_tag="ACHUOY_002237"
     CDS             459..1385
                     /locus_tag="ACHUOY_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR0683004.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1335..2528)
                     /locus_tag="ACHUOY_002238"
     CDS             complement(1335..2528)
                     /locus_tag="ACHUOY_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0683005.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2664..4388
                     /gene="iucA"
                     /locus_tag="ACHUOY_002239"
     CDS             2664..4388
                     /gene="iucA"
                     /locus_tag="ACHUOY_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR0683006.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4389..5336
                     /gene="iucB"
                     /locus_tag="ACHUOY_002240"
     CDS             4389..5336
                     /gene="iucB"
                     /locus_tag="ACHUOY_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR0683007.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5336..7078
                     /gene="iucC"
                     /locus_tag="ACHUOY_002241"
     CDS             5336..7078
                     /gene="iucC"
                     /locus_tag="ACHUOY_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR0683008.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7075..8412
                     /gene="iucD"
                     /locus_tag="ACHUOY_002242"
     CDS             7075..8412
                     /gene="iucD"
                     /locus_tag="ACHUOY_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR0683009.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8418..10613
                     /gene="iutA"
                     /locus_tag="ACHUOY_002243"
     CDS             8418..10613
                     /gene="iutA"
                     /locus_tag="ACHUOY_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR0683010.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDFLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11164..11307)
                     /locus_tag="ACHUOY_002244"
     CDS             complement(11164..11307)
                     /locus_tag="ACHUOY_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683011.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2925 a         2895 c         2950 g         2771 t
ORIGIN      
        1 tcaacgaagg ggccgacaaa cacgttgtcc ctgagttcac attcataaat gttgaccggt
       61 ttgacaatac ggacattgat cccataaatc acatcccgga cagcacattc cagaaatttc
      121 atactcacac gtcctttaaa tacagatgac atttgccgga attataccgc ccagattcac
      181 ttgccatgtt tcagaatagc ttataagccg cacttttacc gatactcagc aacaaatcag
      241 gtctgtatcc acttcagccg gaaaccggat ctgcgcggcg gggaacgggc aagaaatatg
      301 ataattaagc aacacacata aaatcatatt gcataatcgc atctgaatcg aacacttatt
      361 cgcatcacta tcgatcatca cttcgcatct cataccctta cccacaagtt gaaataatag
      421 tattatattg aaaagacaga gattatttca gaggtgttat ggaaaactgg gttattcagg
      481 agctaaaatc actggatata ggggactcaa gacttgataa aagagtaaaa catatcctca
      541 atagcctcag tcgttcacca gaagaaagta ttcctgtttc ctgccgtacc tggagtgaaa
      601 caaaggcagc ttatcgttgt ttttcaaacg ataaagtctc tgctaataaa attatggctc
      661 ctcacaaaga aaatatcact gaaagagctc agcagtttaa aagagtttta gttttacagg
      721 atacaacaga acttaattat tctggtcaga aagaaaaaca aggagttggt cacaaaaaac
      781 ataaagatga aagaaactta tttcttcatc ctcaactcgt catcagtgag tcaggtgttt
      841 gcctcggtgt ttatgatgac tatcagtggt tcagggatga attaaaaacc agtaaaaata
      901 cccgacaaga aatctgcaat gatttacttc ataaaaaaca tgtcagtgaa aaagaaacct
      961 ggcgttgggt tgaaggatat aataaagcaa cagaactggc tcgccaatgc cctgataccc
     1021 atgtattgtc aatttctgat cgtgaaggcg atttttatga cctgtttgaa cgcgcagagc
     1081 agactcccgg aataaaagct gactggcttg tcagaatgaa atttaataac agagcaacgc
     1141 tgaatattaa tggtaaacgg gatcataggt tgttgcacga aagaatcatg gagattacac
     1201 cgcagcaact ggttgaattc actatacctg atgggcgggg gcagcaagcc agaacactgc
     1261 ctgacatgct ttcatcagac gcaggtcctc tctcatatcc tctgatttac tccagtaagg
     1321 tgatgatcat atctttattt gatagtacgg taatgccaga taaatacaga cacaatggta
     1381 aataatatag ttgcaaataa tgctgccata atcagcgcga ttgcgacacc tgttttcgat
     1441 accagcggca ccagcaaagc gcacagtcca tagccaagtg tgtgactggt agcgagccag
     1501 cccgcgcctg cgccatcagc cagcttatca ttcagtagaa gttgataagc gggtgttgcc
     1561 agagctgctc caaatgacag cactgcacag cccgggtaaa acaaccatag ctgttcggag
     1621 agcatgatag ccagaccacc actcatcaac actccggcac tcaggagcag ggccaccgga
     1681 gtcaggcgct gcggacggag taccccgaac tgcgcgataa gcgcagctac tgcggacaga
     1741 cccaacagcc aggccacctg ttggctaatg gcggtggtat ccgtgacgaa ctggcgagta
     1801 agggcaggcg aaagtccaag ctgcatcatg ctcaccgccg cagccagtaa tattgcgcaa
     1861 agcagataag gcagacaatc ccgcttcaga ctgacgctct tgcactccgg tgtgggctgt
     1921 ggtggtgtgc cgggcaaccg cagcagcatc agcagcgcca gcactggtgc tgccatcagt
     1981 agccccagtg gcgccagagg atgaatggcc agcattgctg ccgcgcacag cggcccgaat
     2041 agcctgccgc aactcaggcc ggagctgatg gttgccagag cggccatgcg attcccttct
     2101 ccagctctct gcaatgccca gacctgacag gctggcacca ttgccgacac ggtcagcccg
     2161 taggcgatcc gggcgatgat taatattccc agtcccaccg ctgttgttat ggcgctggta
     2221 gccatcagca cgcttcccag ccctagcagg gtaaagctta tgccatatcc tcccagagcc
     2281 cacaacacca caggtttact gccagtacgg gaaatttgct ttccccacca cggggacgat
     2341 ggcagaaaca gcattgagcc aagcatcagc agggcagccc atacagacag actcagattt
     2401 gtctgtatga ccagaacagg gagcacaacc agcagaccgt tctgtccgat accgagaagc
     2461 ccggcactga acgcaagtgg ccagcaggac agtgtttttt ggggcgtatc ttcgatccct
     2521 ggtgacatta taatgtttca actccatgta ttaattgtgt ttatttgtaa aattaattta
     2581 tctgacaata acatttattg ttgataatga gaatcattat cgacataatt gttattattt
     2641 tactgtgtgg gagctgtttg actatgaccc tgccctctga aaaaccagcc acagatgtgg
     2701 ctgcgcagtg cttcctgaat gcactgattc gtgaaaccac agactggaaa ctgacagaat
     2761 acccgccaga cgaattgctc atcccgctgg atgagcagaa atcgctccat ttcagagtgg
     2821 cttatttctc cccaacccag catcaccgct ttgcatttcc tgcacgtctg gtcacggcat
     2881 caggcagtta tcctgtcgac tttaccactc tctcccggct tattattgat aagctacgcc
     2941 atcaactgtt tctgcccgtt cccctctgcg aaactttcca ccagcgcgtg ctggaaagcc
     3001 atgtccatac gcaacaggca attgatgccc gtcatgactg ggccgctctg cgtgaaaaag
     3061 cgttgaattt tggcgaggct gagcaggcgc tgctgacagg acacgctttc caccctgcgc
     3121 ctaagtctca tgaaccgttt aaccgacggg aggctgaacg ctacctgcct gatatggcac
     3181 cccacttccc actgcggtgg ttttcggtgg ataaaacgca aatcgccggt gaaagtttgc
     3241 atctcaacct tcaacagcgg ctgacgcgat ttgccgcaga gaatgcgcct cagttactca
     3301 acgaattaag tgacaaccaa tggctgttcc cgttgcaccc gtggcagggg gaatatcttt
     3361 tgcagcaggg gtggtgccag gcacttgttg ctaaagggct gattaaagac ttaggtgagg
     3421 ccggcacgtc gtggctgccg accacctctt cccgttccct ctactgtgcc accagccgcg
     3481 atatgatcaa gttctccctg agcgtacggc tgaccaactc catccgtact ctgtccgtga
     3541 aagaagtgaa gcgtgggatg cgcctggcac gcctggctca aaccgacggc tggcagatgc
     3601 tacaggtccg cttcccgact ttccgggtaa tgcaggagga tggctgggcc gggctgctcg
     3661 atcttaacgg caacatcatg caggaaagtc tgtttgccct gcgtgaaaat ctgctggtgg
     3721 atcagccgaa aagccagact aacgtactgg tctccctgac tcaggccgca cctgatggcg
     3781 gtgattcgct gctggtttcg gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc
     3841 aacaggccgc ccatgcatgg gtcgatgcat actgtcagca agtgctaaag ccgctgttta
     3901 cggctgaagc ggattacggc ctggtgctgc tggcgcatca gcaaaatatt cttgtccaga
     3961 tgcttgggga tctgccggtc ggatttattt accgtgactg tcagggcagc gcttttatgc
     4021 ctcacgcgac agactggctc gattctattg gcgaggcgca ggcggaaaat attttcaccc
     4081 atgagcagtt gctgcgctat ttcccttatt acctgctggt taactccact tttgctgtga
     4141 ccgctgcgct gggggctgcc gggctggaca gcgaatcgaa tctgatggct cgtgtacgag
     4201 catcgctggc tgaagtgcgt gatcaggtga ctcataaaac ctgtctcaac tacgtgctgg
     4261 aaagtccgta ctggaacgta aaaggtaact ttttctgtta tctgaacgat cataacgaga
     4321 acaccattgt tgacccttcg gtgatctatt tcgatttcgc taacccgctg caggctcagg
     4381 aggtctgaat gtctgaggca aacattattc acagcagata tggactgcgc tgtgaaaaac
     4441 tcgacaagcc cctgaatctt ggctggggac tggacaatag cgcggtgttg cactgtcccg
     4501 gggagctgcc gacagggtgg ctgtgcgatg cgctggatca gatatttatc gccgcaccac
     4561 aactgtcagc agtggctctc ccctgggccg aatggcgtga ggagccacag gcgctgacgc
     4621 tttttgggca ggtaaaaagc gacattatcc atcgtactgc cttctggcag ttaccgttat
     4681 ggttgagttc accggcaaac cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga
     4741 tttatttccc gcagcgccca ccccgtccac agggtgaggt ttaccgccgt tacgatccac
     4801 gtattcgcag gatgctgagt ttccgcattg ccgatcccgt ttctgatgca gaacgtttca
     4861 cccgctggat gaatgatccg cgcgttgagt atttctggga gcaaagtggc tcactggagg
     4921 tacagaccgc ctatctggag cgccagctca ccggtaaaca tgcattcccg ctgattgggt
     4981 gcttcgacga tcggccattc agctatttcg aaatctactg ggcggcggaa gaccgcattg
     5041 gtcgtcacta ttcgtggcag ccctttgacc gtggcctgca tctgctggtt ggtgaacagc
     5101 aatggcgcgg ggcccactat gtgcaaagct ggctgcgcgg gctgacacat tacctgctgc
     5161 tggatgagcc ccgtacgcag cgcacagtac tggagccacg caccgataac cagcgcctgt
     5221 tccgccatct tgagcctgcg ggataccgga caattaaaga gttcgacttc ccacataagc
     5281 gctcgcgcat ggtgatggcg gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa
     5341 tcacaaggac tgggattttg ttaaccgtcg gctggtggca aaaatgttgt ctgagatgga
     5401 gtatgagcag gttttccacg cggaatctca gggcgatgac cactactgca ttaacctgcc
     5461 gggagcacaa tggcgcttca tcgctgaacg tggtatctgg ggctggctgt ggattgatgc
     5521 ccaaacgttg cgctgcacgg acgaaccagt actggcgcag acgctgctga tgcagctaaa
     5581 gccggtgctg tcaatgagtg atgccactgt tgcagagcat atgcaggatt tgtatgccac
     5641 gctactgggc gacctgcaac tgctgaaggc ccgtcgcgga ctgagtgcca gtgacctgat
     5701 taatcttgat gccgatcgtc tgcaatgcct gctgagcggt catcctaaat tcgtttttaa
     5761 taaaggtcgc cgtggctggg gcaaagaggc gctggaacga tatgcgccag agtataccaa
     5821 caccttcaga ctgcactggc tggcggtaaa acgtgaacat atgatctggc gctgcgataa
     5881 cgatctggat attcagcagt tgttgactgc cgcaatggat ccgcaggagt ttacacgctt
     5941 cagtcaggtc tggcaggaaa acgggctgga tcacaactgg ctaccgctgc cggtacatcc
     6001 gtggcagtgg cagcaaaaaa tcgccacaga cttcatcgct gattttgtcg aaggcaggat
     6061 ggtgtctctc ggcgagtttg gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac
     6121 caacgccagc cgccggggag ggctggatat caagctgccg ctgaccatct acaacacctc
     6181 atgctaccgg ggaatccctg gcagatatat cgctgccgga ccactggctt cacgctggct
     6241 gcaacaggtt tttgcgaccg acgccaccct ggtgcaaagc ggcgcagtga tccttggtga
     6301 accggctgca ggctatgtgt cccatgaagg ctatgccgcg cttgcccggg ctccctatcg
     6361 ctaccaggaa atgcttggtg ttatctggcg ggagaatccg tgccgctggc tgaaaccgga
     6421 tgaaagtcct gttctgatgg caacactgat ggagtgcgac gaaaacaatc agccgctggc
     6481 aggcgcatat atcgaccgct ccggactgga cgctgaaacc tggcttacgc aattgttccg
     6541 ggtggttgtg gttccgctgt atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca
     6601 cggacaaaat ataacgctcg ccatgaaaga gggggtacca cagcgtgttc tgctgaaaga
     6661 cttccagggc gatatgcggc tggtgaaaga agcgtttccc gaaatggact ctttgcctca
     6721 ggaggtgcgt gatgccacat cccgtctgag tgcggactac ttaatccatg atttgcagac
     6781 gggtcacttc gtgacagtac tgcgttttat ttcgccactg atggttcgtc ttggcgtacc
     6841 tgaaaggcga ttttatcaac tgctggcagc agtgttgagt gattacatga acaaacatcc
     6901 acaaatggca gagcgttttg cgcttttctc actcttcagg ccacaaatca ttcgcgtggt
     6961 gctgaacccg gtaaaactga cctggccgga cctggatggc ggcagtcgca tgctgccgaa
     7021 ttaccttgag aatctgcaaa atccgctgtg gctggtaact caggaatatg aatcatgaaa
     7081 aaaagtgtcg attttattgg tgtcgggaca gggccattta atctcagcat tgctgcattg
     7141 tcacatcaga tcgaagaact gaactgtctc ttctttgacg aacatcctca tttttcctgg
     7201 catccgggta tgctggtacc ggattgtcat atgcagaccg tctttctgaa agatctggtc
     7261 agtgcagttg cacctacaaa tccctacagc tttgttaact atctggtgaa gcacaaaaag
     7321 ttctatcgct tccttacaag caggctacgt acagtatccc gtgaagagtt ttctgactat
     7381 ctccgctggg ctgctgaaga tatgaataac ctgtatttca gtcataccgt tgaaaatatt
     7441 gatttcgaca aaaaaagtcg attgtttctg gttcagacca gtcggggaga atattttgcc
     7501 cgcaatatct gccttggtac aggaaaacaa ccttatttac caccctgtgt gaagcatgtg
     7561 acacaatcct gtttccatgc cagtgaaatg aatcttcgtc ggccggacct gagtggaaaa
     7621 cggataaccg tggttggtgg aggacagagt ggtgcagacc tgttccttaa tgcattacgc
     7681 ggggaatggg gagaagcggc ggaaataaac tgggtctcac ggcgtaataa ttttaacgca
     7741 ctggatgagg ctgcttttgc tgatgagtat tttacacctg aatatatttc aggcttctcc
     7801 ggactgaagg aagatattcg ccatcagtta ctggatgagc agaaaatgac atcggatggc
     7861 atcactgccg attctttact gaccatttat cgtgagttgt accaccgttt tgaagttctg
     7921 agaaaaccaa gaaatatccg tctgctaccc agtcgctcgg taacaaccct ggaaagtagt
     7981 ggtccgggct ggaggttatt gatggaacat catctggatc agggcagaga gagcctggaa
     8041 agtgatgtgg tgattttcgc cacaggttac cgttctgcat tgccacaaat acttccctca
     8101 ctgatgccct tgatcaccat gcacgataag aacaccttta aagtgcgtga tgacttcact
     8161 ctggaatgga gtggcccgaa agaaaataac atctttgcgg tcaacgccag catgcaaact
     8221 catggcatcg ccgaacccca gctcagcctg atggcctggc gatctgcacg tattcttaat
     8281 cgcgtactgg ggcgtgattt attcgatctc agtatgccgc ccgcactgat tcagtggcgc
     8341 agcggcagcc ggaaaaaacc gcagccggag gctgcttctt taactcacta tacagcaaat
     8401 attcaggaat aacgatgatg cgcaaaaagt atatgccccg ggctcttggt ccgctgcttc
     8461 ttgtcgtgct gtcaccagct gtcgcccagc aaaacgatga taatgagatc atagtgtctg
     8521 ccagccgcag caatcgaact gtagcggaga tggcgcaaac cacctgggtt atcgaaaatg
     8581 ccgaactgga gcagcagatt cagggcggta aagagctgaa agacgcactg gctcagttaa
     8641 tccccggcct tgatgtcagc agccagagtc gaaccaacta cggtatgaac atgcgtggcc
     8701 gcccgctggt tgtcctgatt gacggtgtgc gcctcaactc ttcacgttcc gacagccgac
     8761 aactggactc tgtcgatcct tttaatatcg accatattga agtgatctcc ggcgcgacgg
     8821 ccctgtacgg tggcgggagt accggagggt tgatcaacat cgtgaccaaa aaaggtcagc
     8881 cggaaaccat gatggagttt gaggctggca caaaaagtgg ctttaacagc agtaaagatc
     8941 acgatgagcg cattgccggt gctgtctccg gcggaaatga ccatatctcc ggacgtcttt
     9001 ccgtggcata tcagaaattt ggcggctggt ttgacggtaa cggcgatgcc accctgcttg
     9061 ataacaccca gaccggcctg cagcactcca atcggctgga catcatggga accggtacgc
     9121 tgaacatcga tgaatcccgg cagcttcaac tgataacgca gtactataaa agtcagggag
     9181 acgacaatta cgggcttaat ctcgggaaag gcttttccgc catcagcggg agcagcacac
     9241 catacgtcag taaggggctg aattctgacc gcattcccgg cactgagcgg catttgatca
     9301 gcctgcagta ctctgacagt gatttcctgg gacaggaact ggtcggtcag gtttactacc
     9361 gcgatgagtc gttgcggtac tacccgttcc cgacggtaaa tgcgaataaa caggcgacag
     9421 ctttctcctc gtcacagcag gataccgacc agtacggcat gaaactgact ctgaacagcc
     9481 aactgatgga cggctggcaa atcacctggg ggctggatgc tgagcatgag cgctttacct
     9541 ccaaccagat gttcttcgat ctggctcagg caagtgcttc cggagggctg aacaaccata
     9601 agatttacac caccgggcgc tatccgtcat atgacatcac caatctggcg gccttcctgc
     9661 aatccagcta tgacattaat gatattttta ccgttagcgg tggcgtacgc tatcagtata
     9721 ctgagaacag ggtagatgat ttcatcgact acacgcagca acagaagatt gctgccggga
     9781 aggcgatatc tgccgacgcc attcctggtg gttcggtaga ttacgataac tttctgttca
     9841 atgctggtct gctgatgcac atcaccgaac gtcagcaggc atggttcaat ttttcccagg
     9901 gggtggcatt gccggatccg gggaaatatt atggtcgcgg catctatggt gcagcagtga
     9961 acggccatct ttccctgaca aagagcgtga acgtcagcga cagtaagctg gaaggcgtga
    10021 aagtcgattc ttatgaactg ggctggcgct ttaccggtga caacctgcgg actcaaatcg
    10081 cggcatatta ctcgctttcc aataagagcg tggaaaggaa taaagatctg accatcagtg
    10141 tgaaggacga caggcgccgt atttacggcg tggaaggtgc ggtggacttc ctgatcccgg
    10201 atactgactg gagtaccggt gtgaacttca atgtgctgaa aaccgagtcg aaagtgaacg
    10261 gtcaatggca aaaatatgac gtgaaggaat caagtccatc gaaagcgaca gcttacatta
    10321 actgggcgcc ggaaccgtgg agtctgcgtg tacagagcac cacttctttc gacgtaagcg
    10381 atgcagaggg taacgatatt aatggttaca ctaccgtcga ttttatcagt agttggcagc
    10441 ttccggtggg aacactcagc ttcagcgttg agaacctctt cgaccgtgac tataccactg
    10501 tctggggaca gcgtgcacct ctgtactact gcccgggtta cggccctgct tcactgtacg
    10561 actacaaagg ccggggccga acctttggtc tgaactactc agtgctgttc tgacggggat
    10621 gcttttgcaa caagggtatg ctgtaaacaa catggtcctt ggtggccatg ttgtttacag
    10681 ccagtataat atacagcctt aacaagatat tccgtctccg gaatggactg ttcaccactg
    10741 aataacatta tttctgtttt atggatcttt gactgaaatt tatcgcatat cctcatttca
    10801 ttgtccgtct gaaaactaac cttacaagta agcgtcgtct cagcaccgtc tggcagatcc
    10861 tgaaattcct gagaggatga tctgcgccca atatcctgag ccggaacttt gctgcaatga
    10921 aggccaacga aaaatggggg agtatgttat agagtacttg ctacagggta aaaagaggta
    10981 cctgtcgctg gttctccgtt cagtttaacc gggagatttt ctcctgcgcc tgtcataaag
    11041 tccggtaatg gagattgtta atacgatggt gcacgatgca ttgtcggtgc ccggcccgga
    11101 ggatgccccg taactacata cggatcaggg ttggcaatat cgaatggcag gctataggca
    11161 aagttaaagc cccatggagt agcacaaaat atgccacaca aaggaaacgg tctgaataac
    11221 gcagtgatgg agaacttctt cggcacactg caaaatcgca tcgtggtcga tcgctaattc
    11281 gccgtgaatc actgaacgat gttccatgcg aacagatatt cgatcatatc gaaatattaa
    11341 acattacccc tccccgcatt gggcaaaaaa aaaacaaaaa acgatcaatt agtattgatt
    11401 cacataatct ctcatcatta atgagaacat tgtcacatat ttacaatata gaactgttac
    11461 ttttagtaag ctgttagtaa ccaactgtta ctaataattt taattcttaa tactttgttc
    11521 attcaataaa tgagttgaga g
//
LOCUS       DAZOYL010000115        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZOYL010000115 DAZOYL010000000
VERSION     DAZOYL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.115"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUOY_002245"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUOY_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR0683012.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUOY_002246"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUOY_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR0683013.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUOY_002247"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUOY_002247"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR0683014.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUOY_002248"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUOY_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR0683015.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHUOY_002249"
     CDS             complement(3928..5214)
                     /locus_tag="ACHUOY_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR0683016.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHUOY_002250"
     CDS             complement(5235..6026)
                     /locus_tag="ACHUOY_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR0683017.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUOY_002251"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUOY_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR0683018.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUOY_002252"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUOY_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR0683019.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUOY_002253"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUOY_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR0683020.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUOY_002254"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUOY_002254"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR0683021.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUOY_002255"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUOY_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR0683022.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHUOY_002256"
     CDS             complement(9882..10364)
                     /locus_tag="ACHUOY_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR0683023.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUOY_002257"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUOY_002257"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR0683024.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2803 a         2747 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZOYL010000116        11355 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZOYL010000116 DAZOYL010000000
VERSION     DAZOYL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.116"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..535)
                     /gene="espL1"
                     /locus_tag="ACHUOY_002258"
     CDS             complement(<1..535)
                     /gene="espL1"
                     /locus_tag="ACHUOY_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR0683025.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQY"
     gene            1060..2988
                     /gene="thrS"
                     /locus_tag="ACHUOY_002259"
     CDS             1060..2988
                     /gene="thrS"
                     /locus_tag="ACHUOY_002259"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR0683026.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2992..3534
                     /gene="infC"
                     /locus_tag="ACHUOY_002260"
     CDS             2992..3534
                     /gene="infC"
                     /locus_tag="ACHUOY_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR0683027.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3631..3828
                     /gene="rpmI"
                     /locus_tag="ACHUOY_002261"
     CDS             3631..3828
                     /gene="rpmI"
                     /locus_tag="ACHUOY_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR0683028.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3881..4237
                     /gene="rplT"
                     /locus_tag="ACHUOY_002262"
     CDS             3881..4237
                     /gene="rplT"
                     /locus_tag="ACHUOY_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR0683029.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4354..4478
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4360..4404
                     /gene="pheM"
                     /locus_tag="ACHUOY_002263"
     CDS             4360..4404
                     /gene="pheM"
                     /locus_tag="ACHUOY_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR0683030.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4687..5670
                     /gene="pheS"
                     /locus_tag="ACHUOY_002264"
     CDS             4687..5670
                     /gene="pheS"
                     /locus_tag="ACHUOY_002264"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR0683031.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5685..8072
                     /gene="pheT"
                     /locus_tag="ACHUOY_002265"
     CDS             5685..8072
                     /gene="pheT"
                     /locus_tag="ACHUOY_002265"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR0683032.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8077..8376
                     /gene="ihfA"
                     /locus_tag="ACHUOY_002266"
     CDS             8077..8376
                     /gene="ihfA"
                     /locus_tag="ACHUOY_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR0683033.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8477..9457
                     /gene="btuC"
                     /locus_tag="ACHUOY_002267"
     CDS             8477..9457
                     /gene="btuC"
                     /locus_tag="ACHUOY_002267"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR0683034.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9520..10071
                     /gene="btuE"
                     /locus_tag="ACHUOY_002268"
     CDS             9520..10071
                     /gene="btuE"
                     /locus_tag="ACHUOY_002268"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR0683035.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10071..10820
                     /gene="btuD"
                     /locus_tag="ACHUOY_002269"
     CDS             10071..10820
                     /gene="btuD"
                     /locus_tag="ACHUOY_002269"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR0683036.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10898..>11355
                     /locus_tag="ACHUOY_002270"
     CDS             10898..>11355
                     /locus_tag="ACHUOY_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR0683037.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2873 a         2631 c         3055 g         2796 t
ORIGIN      
        1 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg
       61 cactaccatt gagatttact gttggttgta tagttttttc tctatggatt tttttagaca
      121 gtttcataca atggttacga tcttcggcag ttaactgttc ctgttcaata taatccagta
      181 tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt
      241 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
      301 tgataaaaag ggcagtggaa taacaatttt gtgaaatttt tcgacagttt gcggtaaatt
      361 cctgagtaac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
      421 gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt
      481 gcaatctatg aggagactta taatgggttc tgataatgat atcgttttga ctcataatct
      541 attcgcctta tatatgtctg gaggttttga ggatgatgat taattattat cgccagaacg
      601 aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac
      661 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
      721 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
      781 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
      841 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg
      901 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
      961 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
     1021 tctttcgtgt gggtcaccac tgcaaattaa ggatataaaa tgcctgttat aactcttcct
     1081 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
     1141 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
     1201 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
     1261 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
     1321 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
     1381 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
     1441 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1501 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1561 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1621 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1681 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1741 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1801 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1861 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1921 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1981 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     2041 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     2101 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     2161 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     2221 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     2281 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     2341 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     2401 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     2461 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2521 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2581 gtaatgattc accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa
     2641 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2701 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2761 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2821 cgtcgcgtcc catatatgtt ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2881 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2941 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     3001 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     3061 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     3121 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     3181 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     3241 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat
     3301 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     3361 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     3421 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg
     3481 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3541 caagtagcac tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt
     3601 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3661 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3721 cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3781 tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3841 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3901 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3961 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     4021 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     4081 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     4141 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     4201 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     4261 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     4321 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     4381 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     4441 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4501 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
     4561 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4621 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4681 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4741 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4801 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4861 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4921 gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgctaggt
     4981 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     5041 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     5101 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     5161 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     5221 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
     5281 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     5341 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
     5401 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
     5461 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
     5521 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5581 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5641 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5701 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
     5761 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5821 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5881 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg tggtgcgcca aactgccgtc
     5941 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     6001 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttc tctgaactgg
     6061 gaatttctga cgatcacagc ggcattatcg aactgcctgc agatgcgccg attggcactg
     6121 acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgta acgccaaacc
     6181 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     6241 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     6301 cagtcgaagc gcctgaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     6361 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     6421 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6481 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6541 cgctggtact gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6601 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6661 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6721 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6781 cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6841 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6901 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgct gatgaacagg
     6961 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
     7021 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtt gaagaagtcg
     7081 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     7141 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     7201 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     7261 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     7321 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     7381 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     7441 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7501 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
     7561 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7621 cggggcaatc cgcagcgatt tatctgaaag gggaacgtat tggttttgtt ggagttgttc
     7681 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
     7741 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7801 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7861 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7921 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7981 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     8041 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     8101 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     8161 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     8221 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     8281 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     8341 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     8401 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     8461 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8521 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8581 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8641 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8701 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8761 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8821 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8881 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8941 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     9001 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     9061 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     9121 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     9181 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     9241 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     9301 acttcccggc tgcgcgctgg caggggcgag cgcagtgctg ctggccgata ttgtagcgcg
     9361 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     9421 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9481 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9541 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9601 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9661 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9721 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9781 ggggtgacgt tcccgatgtt cagtaagatt gaagttaacg gcgaaggacg ccatccgctg
     9841 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9901 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
     9961 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    10021 ccggaaaacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    10081 tgatgcagtt acaggatgtt gcggaatcta cccgcctagg gccactttct ggcgaggttc
    10141 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
    10201 cacgaatggc cggaatgacc agcggtaagg gaagtattca gttcgcgggg caaccactgg
    10261 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    10321 cgccgtttgc aatgccggtc tggcactatc tgacactgca tcagcacgat aaaacgcgta
    10381 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggacgtagca
    10441 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    10501 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10561 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    10621 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    10681 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10741 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10801 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    10861 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10921 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10981 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    11041 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    11101 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    11161 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    11221 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    11281 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    11341 ctggcaggcg agacg
//
LOCUS       DAZOYL010000117        11242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZOYL010000117 DAZOYL010000000
VERSION     DAZOYL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.117"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            3..308
                     /locus_tag="ACHUOY_002271"
     CDS             3..308
                     /locus_tag="ACHUOY_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR0683038.1"
                     /translation="MLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="ACHUOY_002272"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="ACHUOY_002272"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR0683039.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHUOY_002273"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHUOY_002273"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR0683040.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHUOY_002274"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHUOY_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR0683041.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHUOY_002275"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHUOY_002275"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR0683042.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHUOY_002276"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHUOY_002276"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR0683043.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHUOY_002277"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHUOY_002277"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR0683044.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHUOY_002278"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHUOY_002278"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR0683045.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHUOY_002279"
     CDS             complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHUOY_002279"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR0683046.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHUOY_002280"
     CDS             complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHUOY_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR0683047.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHUOY_002281"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHUOY_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR0683048.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..11242
                     /locus_tag="ACHUOY_002282"
                     /pseudo
     CDS             10353..11242
                     /locus_tag="ACHUOY_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2554 a         3090 c         2950 g         2648 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtga aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAZOYL010000118        11208 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZOYL010000118 DAZOYL010000000
VERSION     DAZOYL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.118"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHUOY_002283"
     CDS             53..352
                     /locus_tag="ACHUOY_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR0683049.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHUOY_002284"
     CDS             complement(472..651)
                     /locus_tag="ACHUOY_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR0683050.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHUOY_002285"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHUOY_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR0683051.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHUOY_002286"
     CDS             2090..2668
                     /locus_tag="ACHUOY_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR0683052.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUOY_002287"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUOY_002287"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0683053.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHUOY_002288"
     CDS             4347..5945
                     /locus_tag="ACHUOY_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0683054.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHUOY_002289"
     CDS             complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHUOY_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR0683055.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHUOY_002290"
     CDS             complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHUOY_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR0683056.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7962..8933
                     /gene="manX"
                     /locus_tag="ACHUOY_002291"
     CDS             7962..8933
                     /gene="manX"
                     /locus_tag="ACHUOY_002291"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR0683057.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8996..9796
                     /gene="manY"
                     /locus_tag="ACHUOY_002292"
     CDS             8996..9796
                     /gene="manY"
                     /locus_tag="ACHUOY_002292"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR0683058.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9809..10660
                     /gene="manZ"
                     /locus_tag="ACHUOY_002293"
     CDS             9809..10660
                     /gene="manZ"
                     /locus_tag="ACHUOY_002293"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR0683059.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10715..11173
                     /locus_tag="ACHUOY_002294"
     CDS             10715..11173
                     /locus_tag="ACHUOY_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR0683060.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2753 a         2822 c         2829 g         2804 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca gctcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggg ttatcggcat cgtgctgaac attggcctga
     6721 cgtttcccgc ccatcaaacg caggatggca tcggcagtac gcgctcgcag cggcaaagtc
     6781 gactggtggc gaataaagtt gcgacgcgca atctggttaa acacttcgat gatgatcgag
     6841 aagccaatcg cggcatacag gtaacctttc ggaatgtgga aaccgaaacc ttctgccacc
     6901 agactcagac caatcattaa caggaagctc agacagagca ccaccaccgt ggggtgctgg
     6961 ttaacgaatc gcgtcagcgg tttggatgcc agcaacataa ccgccatcgc aatcactacc
     7021 gccgccatca tcaccggcag atggttaacc atccctactg cagtaattac cgcatccaac
     7081 gagaagacgg cgtcaaggat gacaatctgt gtgacgacca cccagaaact ggcgtagcct
     7141 ttaccgtggc cggaatcatg atcgcggttt tccagccgtt catgcagttc ggttgttgct
     7201 ttgaacagca agaatatccc cccgaacaac ataatcaggt cgcgtccgga gaaggagaaa
     7261 tccatgacgg taaatagcgg tttggtcagc gtgaccatcc atgaaatcag cgacagcagc
     7321 cccagacgca taatcagcgc cagtgataac cccagcaaac gcgctttatc gcgttgtttt
     7381 ggcggcagtt tgtcagcaag aatggcgatg aagaccaggt tatcgatacc cagcacaatt
     7441 tcgagaacaa caagcgtgag tagccccgcc caaattgagg ggtccattaa gaattccatg
     7501 acaagctcct gcttaaggaa tagctattcg acgccagaaa taatgcaggc gtaacgacaa
     7561 aatgcaaacg aaaggtgcgg catagagtgc cagaaaggca ggcgttaaaa ggcctgatgc
     7621 tgaaatgacg tcggtgacga tccatactgc gggctactgc cctatactcc atggttgtta
     7681 aacgggagtt aaacatatca gagacgcctc tgatttggca aagatttacc ttcctttgca
     7741 aacgaatgtg acaaggatat tttacctttc gaaatttctg ctaatcgaaa gttaaattac
     7801 ggatcttcat cacataaaat aattttttcg atatctaaaa taaatcgcga aacgcagggg
     7861 tttttggttg tagcccttat ctgaatcgat tcgattgtgg acgacgattc aaaaatacat
     7921 ctggcacgtt gaggtgttaa cgataataaa ggaggtagca agtgaccatt gctattgtta
     7981 taggcacaca tggttgggct gcagagcagt tgcttaaaac ggcagaaatg ctgttaggcg
     8041 agcaggaaaa cgtcggctgg atcgatttcg ttccaggtga aaatgccgaa acgctgattg
     8101 aaaagtacaa cgctcagttg gcaaaactcg acaccactaa aggcgtgctg tttctcgttg
     8161 atacatgggg aggcagcccg ttcaatgctg ccagccgcat tgtcgtcgac aaagagcatt
     8221 atgaagtcat tgcaggcgtt aacattccaa tgctcgtgga aacgttaatg gctcgtgatg
     8281 atgacccaag ctttgatgaa ctggttgcgc tggcagtaga aacaggccgt gaaggcgtga
     8341 aagcactgaa agccaaaccg gttgaaaaag ccgcgccagc acccgctgcc gcagcaccaa
     8401 aagcggctcc aactccggca aaaccaatgg ggccaaacga ctacatggtt attggccttg
     8461 cgcgtatcga cgaccgtctg attcacggtc aggtcgccac ccgctggacc aaagaaacca
     8521 atgtctcccg tattattgtt gttagtgatg aagtggctgc ggataccgtt cgtaagacac
     8581 tgctcaccca ggttgcacct ccgggcgtaa cagcacacgt agttgatgtt gccaaaatga
     8641 ttcgcgtcta caacaacccg aaatatgctg gcgaacgcgt aatgctgtta tttaccaacc
     8701 caacagatgt agagcgtctc gttgaaggcg gcgtgaaaat cacctctgtt aacgtcggtg
     8761 gtatggcatt ccgtcagggt aaaacccagg tgaataacgc ggtttcggtt gatgaaaaag
     8821 atatcgaggc gttcaagaaa ctgaatgcgc gcggtattga gctggaagtc cgtaaggttt
     8881 ccaccgatcc gaaactgaaa atgatggatc tgatcagcaa aatcgataag taacgtattg
     8941 tgttgattat cactcagttt tcacacttaa gtcttacgta aacaggagaa gtacaatgga
     9001 gattaccact cttcaaattg tgctggtatt tatcgtagcc tgtatcgcag gtatgggatc
     9061 aatcctcgat gaatttcagt ttcaccgtcc gctaatcgcg tgtaccctgg tgggtatcgt
     9121 tcttggggat atgaaaaccg gtattattat cggtggtacg ctggaaatga tcgcgctggg
     9181 ctggatgaac atcggtgctg cagttgcgcc tgacgccgct ctggcttcta tcatttctac
     9241 cattctggtt atcgcaggtc atcagagcat tggtgcaggt atcgcactgg caatccctct
     9301 ggccgctgcg ggccaggtac tgaccatcat cgttcgtact attaccgttg ctttccagca
     9361 cgctgcggat aaggctgctg ataacggcaa cctgacagcg atttcctgga tccacgtttc
     9421 ttctctgttc ctgcaagcaa tgcgtgtggc tattccggcc gtcatcgttg cgctgtctgt
     9481 tggtaccagc gaagtacaga acatgctgaa tgcgattccg gaagtggtga ccaatggtct
     9541 gaatatcgcc ggtggcatga tcgtggtggt tggttatgcg atggttatca acatgatgcg
     9601 tgctggctac ctgatgccgt tcttctacct cggcttcgta accgcagcat tcaccaactt
     9661 taacctggtt gctctgggtg tgattggtac tgttatggca gtgctctaca tccaacttag
     9721 cccgaaatac aaccgcgtag ccggtgcgcc tgctcaggca gctggtaaca acgatctcga
     9781 taacgaactg gactaacagg tgagcgaaat ggttgataca actcaaacta ccaccgagaa
     9841 aaaactcact caaagtgata ttcgtggcgt cttcctgcgt tctaacctct tccagggttc
     9901 atggaacttc gaacgtatgc aggcactggg tttctgcttc tctatggtac cggcaattcg
     9961 tcgcctctac cctgagaaca acgaagctcg taaacaagct attcgccgtc acctggagtt
    10021 ctttaacacc cagccgttcg tggctgcgcc gattctcggc gtaaccctgg cgctggaaga
    10081 acagcgtgct aatggcgcag agatcgacga cggtgctatc aacggtatca aagtcggttt
    10141 gatggggccg ctggctggtg taggcgaccc gatcttctgg ggaaccgtac gtccggtatt
    10201 tgcagcactg ggtgccggta tcgcgatgag cggcagcctg ttaggtccgc tgctgttctt
    10261 catcctgttt aacctggtgc gtctggcaac ccgttactac ggcgtagcgt atggttactc
    10321 caaaggtatc gatatcgtta aagatatggg tggtggcttc ctgcaaaaac tgacggaagg
    10381 ggcgtctatc ctcggcctgt ttgtcatggg ggcattggtt aacaagtgga cacatgtcaa
    10441 catcccgctg gttgtctctc gcattactga ccagacgggc aaagaacacg ttactactgt
    10501 ccagactatt ctggaccagt taatgccagg cctggtacca ctgctgctga cctttgcttg
    10561 tatgtggcta ctgcgcaaaa aagttaaccc gctgtggatc atcgttggct tcttcgtcat
    10621 cggtatcgct ggttacgctt gcggcctgct gggactgtaa gactgttgta cactaccggg
    10681 gccttttggc cccgtttttt tatctggagg attaatgaca atcacggacc tggtactgat
    10741 tcttttcatc gccgcactcc tggccttcgc gatctacgat cagttcatca tgccccgccg
    10801 taacggcccc accctgctgg caattccttt gctccggcgt ggtcgcatcg atagcgttat
    10861 cttcgtcgga ttgattgtca ttcttatcta taacaacgtc acgaatcatg gtgcgttaat
    10921 aacgacatgg ttattaagcg cactggctct gatgggtttt tatatattct ggatccgcgt
    10981 tccgaagatc atctttaaac aaaaaggttt tttcttcgcc aatgtctgga ttgaatatag
    11041 ccgaatcaaa gcgatgaact tgtcggaaga tggcgtgttg gtgatgcaat tagaacagcg
    11101 tcggctgtta atccgcgttc gaaatatcga cgatctggaa aaaatttata agcttctcgt
    11161 ttcaactcaa taagttatga atttagccaa agctatgttt agtgtatt
//
LOCUS       DAZOYL010000119        11177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZOYL010000119 DAZOYL010000000
VERSION     DAZOYL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.119"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            3..104
                     /locus_tag="ACHUOY_002295"
     CDS             3..104
                     /locus_tag="ACHUOY_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0683061.1"
                     /translation="MIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            83..187
                     /gene="speFL"
                     /locus_tag="ACHUOY_002296"
     CDS             83..187
                     /gene="speFL"
                     /locus_tag="ACHUOY_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR0683062.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            498..2696
                     /gene="speF"
                     /locus_tag="ACHUOY_002297"
     CDS             498..2696
                     /gene="speF"
                     /locus_tag="ACHUOY_002297"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR0683063.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2693..4012
                     /gene="potE"
                     /locus_tag="ACHUOY_002298"
     CDS             2693..4012
                     /gene="potE"
                     /locus_tag="ACHUOY_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR0683064.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4068..5708)
                     /gene="pgm"
                     /locus_tag="ACHUOY_002299"
     CDS             complement(4068..5708)
                     /gene="pgm"
                     /locus_tag="ACHUOY_002299"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR0683065.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5734..6279)
                     /gene="seqA"
                     /locus_tag="ACHUOY_002300"
     CDS             complement(5734..6279)
                     /gene="seqA"
                     /locus_tag="ACHUOY_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR0683066.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6464..7228
                     /gene="ybfF"
                     /locus_tag="ACHUOY_002301"
     CDS             6464..7228
                     /gene="ybfF"
                     /locus_tag="ACHUOY_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0683067.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7368..7661
                     /gene="ybfE"
                     /locus_tag="ACHUOY_002302"
     CDS             7368..7661
                     /gene="ybfE"
                     /locus_tag="ACHUOY_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR0683068.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7801..8331
                     /gene="fldA"
                     /locus_tag="ACHUOY_002303"
     CDS             7801..8331
                     /gene="fldA"
                     /locus_tag="ACHUOY_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR0683069.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8541..8627
                     /gene="uof"
                     /locus_tag="ACHUOY_002304"
     CDS             8541..8627
                     /gene="uof"
                     /locus_tag="ACHUOY_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR0683070.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8620..9066
                     /gene="fur"
                     /locus_tag="ACHUOY_002305"
     CDS             8620..9066
                     /gene="fur"
                     /locus_tag="ACHUOY_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR0683071.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9151..9477)
                     /gene="chiQ"
                     /locus_tag="ACHUOY_002306"
     CDS             complement(9151..9477)
                     /gene="chiQ"
                     /locus_tag="ACHUOY_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR0683072.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9527..10933)
                     /gene="chiP"
                     /locus_tag="ACHUOY_002307"
                     /pseudo
     CDS             complement(9527..10933)
                     /gene="chiP"
                     /locus_tag="ACHUOY_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2715 a         2762 c         2795 g         2905 t
ORIGIN      
        1 tgttgatctt cttcacagat tatagccatt tcatggatag aataactcta ccttcaactg
       61 acacagcaag aggtaaaggt aaatggaaaa taataaccgc ttaatgcctc atataaggcg
      121 gacaacccat atcatgaagt ttgcccatcg taatagtttt gactttcatt tctttaatgc
      181 ccgctagtct tctgactaaa gggcacccca acgtacaggt ctccctgact ttaagcatta
      241 caggttaata cctgtattcc tcggtgctca tatactgcta acccttttaa actctaaata
      301 attcgagtcg cagcacttgc aacttgaggt atgacgagta tagccagtta ccgggctggt
      361 ctgggttatt gcatctgcaa aaagcaaact actgatttat ttatcagcgg tggagctttg
      421 ctttttttcc ggcgtgatcg atttctcctt tgagaaattg aggacctgct attacctgaa
      481 ataaagagat gaataaaatg tcagaattaa aaattgccgt tagccgttct tgcccggatt
      541 gtttttccac tcatcgtgca tgcgtgaata ttgacgaaag taattatatt gacgttgccg
      601 ccattatttt atcagtcaat gatgttgaac gtggaaaact cgatgaaata gacgctactg
      661 gctatggcat tcctgttttt attgcaacgg aaaatgaaga acgtgtacca gcagaatatc
      721 tgccacgtat ttctggtgtc ttcgagcatt gcgaatcacg gaaagagttt tatggacgtc
      781 agttagaaac cgctgctagc cattatgaaa cgcaactgcg cccgcccttc ttccgcgcac
      841 tggtcgatta tgtcaatcag ggcaatagcg cttttgactg cccgggtcat cagggaggtg
      901 agtttttccg tcgtcatccg gcagggaatc agttcgtgga gtacttcgga gaaacgctgt
      961 tccgttccga cttatgcaac gcagacgtag caatgggcga tctgctgatt cacgaaggcg
     1021 cgccatgcat tgcacagcaa catgcggcaa aagtgtttaa tgccgataaa acatacttcg
     1081 ttctaaatgg cacttcatct tctaacaaag tggttttaaa cgccctgcta acaccgggtg
     1141 atctggtgct gtttgatcgc aataaccaca aatctaacca ccacggagcg ttgctacagg
     1201 ctggtgcaac accggtttat ctggaaacgg cacgtaaccc gtatggcttt atcggtggca
     1261 ttgatgcgca ctgttttgaa gaaagttacc tgcgtgagct gatcgcggaa gtcgcaccgc
     1321 agcgggcaaa agaggctcgt cctttccgcc tcgctgtgat tcagttaggc acctacgacg
     1381 gtacgattta taacgcccgc caggtggtgg ataaaattgg tcatctgtgt gactacattc
     1441 tgtttgactc cgcatgggtt ggctatgagc aatttatccc gatgatggct gactgttcgc
     1501 cgctgttgct ggatcttaat gagaacgatc cgggtattct ggttacgcaa tccgtacata
     1561 aacagcaggc tggtttttct caaacttcac aaatccataa aaaagacagc cacatcaaag
     1621 ggcaacagcg ttatgtaccg cacaaacgca tgaacaacgc ctttatgatg cacgcctcca
     1681 ccagcccgtt ctatccgctg tttgccgcac tggatatcaa cgccaaaatg catgaaggtg
     1741 tcagcggtcg taatatgtgg atggattgtg tggtaaatgg cattaatgcc cgcaaactga
     1801 tcctcgataa ctgtcagcat attcgtccgt tcgtacctga actggtggat ggtaaaccct
     1861 ggcagtcgta tgaaacagcg caaattgcgg ttgatctgcg cttcttccag tttgtaccag
     1921 gggaacactg gcattctttt gaaggctatg cagagaatca atactttgtc gatccttgca
     1981 aactgttgct gacaacccca ggtattgatg cacgtaacgg cgaatatgaa gcgttcggtg
     2041 tacccgcgac gattcttgct aacttcctgc gcgaaaatgg cgtagtgccg gaaaaatgcg
     2101 atcttaactc catcctcttc ctgctgactc cggcagaaga tatggccaaa cttcagcaac
     2161 ttgttgccct gctggtacgc ttcgaaaaac tgcttgagtc cgacgcgcca ttagcagaag
     2221 tgctgccttc catctacaaa cagcatgaag agcgctacgc cggttatacc ctgcgtcagt
     2281 tgtgccagga aatgcatgat ttgtatgccc gccacaacgt gaaacaactg caaaaagaga
     2341 tgttccgcaa agcgcacttc ccacacgtca gcatgaatcc gcaagaagcc aactacgcct
     2401 atttacgcgg tgaagtggag ctggttcgtc tgccggatgc cgaaggtcgt atcgctgccg
     2461 aaggggcgct tccttatcct ccgggtgtgc tgtgtgttgt tccgggtgaa atctggggtg
     2521 gtgctgtttt gcgttacttc agcgctctgg aagaagggat caacctgctg ccaggttttg
     2581 caccggagct gcagggtgtc tatatcgaag aacatgatgg tcgtaagcaa gtttggtgct
     2641 atgtcatcaa gcctcgtgat gcgcaaagca ccctgttgaa aggggaaaaa ttatgagtca
     2701 ggctaaatcg aacaaaatgg gcgtcgttca gttaaccata ctgacgatgg tcaacatgat
     2761 gggctccggt atcatcatgc tgccgacaaa gcttgccgaa gttgggacaa tttcaattat
     2821 ctcctggctg gtgacagccg taggctcaat ggcactggca tgggcattcg caaaatgcgg
     2881 tatgttcagc cgtaaatcag gtggtatggg cggttatgcc gagtatgctt tcggtaaatc
     2941 cggtaacttt atggcgaact atacctacgg cgtctcactg ctgattgcta acgtcgcgat
     3001 tgctatttcg gcggttggtt acggcaccga actgctcggc gcgagtttgt cgccagtgca
     3061 gattggtctt gcgaccatcg gggtgctgtg gatttgtacc gtggctaact ttggtggtgc
     3121 gcgcattacc gggcaaatca gtagcattac cgtgtggggg gtcattattc cggtggtagg
     3181 cctgtgcatt atcggctggt tctggtttag cccgacgctt tacgttgatt cctggaatcc
     3241 gcatcatgca ccgttcttca gtgcagtagg ttcttccatc gctatgacgc tgtgggcttt
     3301 tctcggtctg gagtctgcgt gtgcgaatac tgatgtagtg gaaaacccgg aacgtaatgt
     3361 gccaatcgcg gtactcggcg gtacgttagg tgcggcggtg atttatatcg tctccaccaa
     3421 cgtgattgca gggattgtgc ctaatatgga gctggcaaat tcaacggcac catttggcct
     3481 ggccttcgca cagatgttca caccagaagt gggtaaagtc attatggcgc tgatggtgat
     3541 gtcctgctgc ggttcgctgc ttggctggca gttcaccatt gcccaggtgt ttaagtcttc
     3601 atctgatgaa ggctacttcc ctaaaatttt ctcccgtgta accaaagtgg atgcaccggt
     3661 gcagggaatg ttgaccattg tgattatcca gagcgggttg gcgctgatga cgattagccc
     3721 gtcgctaaac agtcagttca acgtgctggt taacctggcc gtggtgacca atatcattcc
     3781 gtatattctg tcaatggcgg cattagtcat tattcagaag gtcgctaatg tgcccccctc
     3841 aaaagcgaaa gttgcaaact ttgttgcttt tgttggcgcg atgtatagct tttatgcgct
     3901 gtactcatcc ggggaagaag ccatgctgta cggttccatc gtgaccttcc ttggttggac
     3961 actgtatggt ctggtatcac cacgctttga actgaaaaat aaacacggtt aaaaaagcaa
     4021 agtaaaaaag ggcgatcttg cgaccgccct ttttttatta aatgtgttta cgcgtttttc
     4081 agaacttcgc taacaatctc aaccgcttct ttctcaatct gcttgcgatg ttcttcaccg
     4141 aggaagcttt cgcagtagat cttatatgcg tcttccgtgc ctgacggacg cgcggcgaac
     4201 cagccgttgt cagtcatcac tttcagaccg ccgatagaag caccattgcc cggtgccgcc
     4261 gtcaggcgcg cggtgatcgg gtcgcctgcc agggtgctgg cgctcaccat ttccggagac
     4321 agcttagaca gcgccgcttt ttgtgcggaa gtcgcagctg cctgcaaacg gttgtagctc
     4381 ggcgcaccaa agcgttttgc cagttcgttg tagtgttcct gcgggttctt accggtgaca
     4441 gcggtgattt ccgccgccag cagacacatg atgatgccgt ctttgtcggt ggaccacggc
     4501 gtgccgtcga aacgcaggaa ggaagcccct gcgctctctt cgccgccaaa gccgaagctg
     4561 ccgtcgaaca gaccatcaac aaaccatttg aaacctaccg ggacttctac cagcttgcgg
     4621 cccaagtcat tgaccacacg gtcgatcatc gcagaagaaa ccagcgtttt accgacagca
     4681 acatctttgc cccactgcgg acgatgctgg aacaggtagt tgatcgccac cgccaggtag
     4741 tggttcggat tcatcaaacc tgccggagtg acgataccgt gacggtcata atccgggtcg
     4801 ttagcaaacg ccagatcgaa cttatcacgc aatgccagca ggcccgccat cgcacactcg
     4861 gaggagcagt ccatacggat cgcgccgtct ttatcgaggt gcataaagcg gaaggtttga
     4921 tcgacctgat cgttaacgat ggtcaggttg aggttgtaat actcgccaat tcgcttccag
     4981 tattcgatac cggaaccacc cagcggatca acgcccagcg tcaggcccgt tttctgaatc
     5041 gccgccatat caacgatatc ggccagacct tccacaaact gctgcaccag atcctgctct
     5101 ttcacatgac cggatgccat cgcttcgtcg agggagatac gcttcacgcc tttcaggcca
     5161 tcggccatca gtgcgttggc acgatcttcc accactttag tgacgttggt atcagccggg
     5221 ccaccatttg gcggattgta cttgatacca ccatcttccg gcgggttatg ggagggtgta
     5281 atcacgatac cgtctgccag cgggccacct tttttattat gaaccaggat ggcattggaa
     5341 acggcaggcg tcggagtgaa gccattgttt tcctgcacaa tgacatcaac gccgttcgct
     5401 gccagcactt ccagtacgga aatgaacgca ggctcggaca gggcgtgagt atctttaccc
     5461 acatagcaag ggccagtgat gccgtttttc gcacgttctt cagcaattgc ctgagcgatc
     5521 gccagaatgt gcggctcgtt aaagctgtgg cgcgctgcac tgccacggtg accggaagta
     5581 ccgaatttca ccgcgtgctc cgcattccct gcttctggtt tcagtacata atattgcgcc
     5641 gtcagttggg cgacgttaat caaatcactc tgttgtgcag gttggcctgc acgattgtgg
     5701 attgccattg ctttgtcctt tgtctgcaac gttttagata gttccgcaaa ccttctcaat
     5761 caattccgcc gggaattgca tcgactgcat gatgtgttcg atcatgctgc atttacggcc
     5821 ggtgttggtg ttggtgatca cccaatacgg cgtgcctggc acatgtttcg gcttggtctg
     5881 attaccattt ttcagcagcg tttgttcatc tgccgcaaag taaacgcgtg tacgaccgtg
     5941 caacgattcc gttgcttcgg caaacgcctg ggcgtcaaga gaatatagtg tagacaacag
     6001 cagcataaag cgattgaccg ctcgcttttg ctctgcgtat tcgtcagaaa gcagaagttc
     6061 acgcattgcg cgaaccttgt ctttaatcgt tttgaccggc ttcgcttcga cgatagcagg
     6121 tgacgcaacg cgaacctctt tcgtcaccgg agcagcaggc tgtgatgcgg cggaaaattt
     6181 caacatacgc cgtaaaatgt cggatgcgct ctcgccgata tgcttagtgt ggctggcaat
     6241 atagctgtag agttcatcat caacttcaat cgttttcatc ttaatccagt acagtgtctt
     6301 agctgaatac aagtcgccaa gagtatatga ataaatcccg gcagcggata gcgtcaagcc
     6361 tggcttgcag aaaaactcgg aataaacctt atttgttgca gccctgtggc acaatggtca
     6421 acataatacc ctaacccgac aacgcgaaaa aaagaacttt gccatgaaat tgaatatccg
     6481 cgcgcaaact gcacaaaacc agcacaataa ttctcccatc gttcttgtcc atggtctgtt
     6541 tggtagcctc gacaaccttg gcgtactggc tcgcgatctg gtaaacgatc acaatatcat
     6601 ccaggttgat atgcgtaacc acggtctttc accgagagat ccggtaatga attacccggc
     6661 gatggcgcag gatcttgttg atactctgga tgcacagcag atcgacaaag caacatttat
     6721 cggtcactcc atgggcggta aagcggtaat ggcacttacg gcactggccc gcgatcgcat
     6781 cgataaactg gtggcgatcg atatcgcacc ggtcgactat cacgtacgcc gtcatgatga
     6841 gattttcgcg gctatcaacg cggtcagtga atcggacgca caaactcgcc agcaagcagc
     6901 agcaataatg caccagcatc ttaatgaaga aggggtgatt cagtttctgt tgaaatcttt
     6961 tgttgacggg gagtggcgct ttaacgtgcc ggtattgtgg gatcagtatc cgcatattgt
     7021 gggttgggag aaaatcccgg catgggatca ccctgccctg tttatccctg gcggcaattc
     7081 tccgtatgtt agcgagcagt accgtgatga tttactggca caatttccac aggcacgggc
     7141 gcatgtgatt gcaggcgcgg gtcactgggt ccatgctgaa aaaccggatg cggtattacg
     7201 cgctatccgt cgctatctca atgattaacg caactgatta aaaacccagc gtccgcgtcg
     7261 ctttccgcgc gtgggcgttg gcgtgactca tcgcgctgat gtattatggc gcgctatcca
     7321 tccgggcaga ggcctggctg attgtttccc ccgaagtcac caagatcatg gccaaagaac
     7381 aaacggaccg tacgacatta gatctgttcg cgcacgagcg tcgaccggga cgaccgaaaa
     7441 ctaatccgct ttcgcgcgat gaacagctgc gtattaataa acgcaaccag ctaaaacgcg
     7501 acaaagtacg tggccttaag cgtgtcgaac tgaagctgaa cgcggaagct gtcgaggcgc
     7561 tgaacgagct ggcggagtcg cgcaatatga gccgtagcga actgatcgaa gagatgttaa
     7621 tgcagcaact ggcggcgctg cgtagtcagg gcatcgttta aatttccact ttcatgtagc
     7681 acagtgtgca gtcctgctcg tttgctgatt ccgcaagact gcctgttctg ctatgattgc
     7741 ctttatccgt gggcaatttt ccacccccat ttcaataagt ttcaagaggt tatttcactc
     7801 atggctatca ctggcatctt tttcggcagc gacaccggta ataccgaaaa tatcgcaaaa
     7861 atgattcaaa aacagcttgg taaagacgtt gccgatgtcc atgacattgc aaaaagcagc
     7921 aaagaagatc tggaagctta tgacattctg ctgctgggca tcccaacctg gtattatggc
     7981 gaagcgcagt gtgactggga tgacttcttc ccgactctcg aagagattga tttcaacggc
     8041 aaactggttg cgctgtttgg ttgtggtgac caggaagatt acgccgaata tttctgcgac
     8101 gcattgggca ccatccgcga catcattgaa ccgcgcggtg caacgatcgt tggtcactgg
     8161 ccaactgcgg gctatcattt cgaagcatca aaaggtctgg ctgatgacga ccactttgtc
     8221 ggtctggcta tcgacgaaga ccgtcagccg gaactgaccg ctgaacgtgt agaaaaatgg
     8281 gttaaacaga tttctgaaga gttgcatctc gacgaaattc tcaatgcctg atgtgatgcg
     8341 gcgtagactc atgtctacgc cgtattaata gataatgcca atcaaaataa ttgctacaaa
     8401 tttgtaactt ttgctgttgt acctgtacaa tgtcccggtg ttcaagtggc cttgccgttg
     8461 taaatgtaag ctgtgccacg tttttattaa caatatttgc cagggacttg tggttttcat
     8521 ttaggcgtgg caattctata atgatacgca ttatctcaag agcaaattct gtcacttctt
     8581 ctaatgaagt gaaccgctta gtaacaggac agattccgca tgactgataa caataccgcc
     8641 ctaaagaaag ctggcctgaa agtaacgctt cctcgtttaa aaatcctaga agttcttcag
     8701 gagccggaca accatcacgt cagtgcggaa gatttataca aacgtctgat cgatatgggt
     8761 gaagaaattg gtctggctac ggtatatcgc gtactgaacc agtttgacga cgctggtatc
     8821 gtcacccgcc acaattttga aggcggtaaa tccgtatttg aactgacaca gcaacatcac
     8881 cacgatcacc tgatctgcct cgactgcggc aaggttatcg aatttagtga tgattccatc
     8941 gaagcgcgtc agcgtgaaat tgccgcaaaa catggcattc gcctgactaa ccacagtctc
     9001 tatctttacg gtcactgtgc cgaaggcgat tgccgcgaag atgaacatgc gcacgaaggc
     9061 aaataagcca gcctgaatga gaaaaagcca acctgcgggt tggctttttt atgcaaggga
     9121 aataagaaaa gcgagagtta cagctctcac ttatttgtta ttactgcgaa tttcctgcca
     9181 aactttatca caatcggctt tcaccgcctg atcatttccg gtttgcgttg cacgcagata
     9241 ctgctgataa tccagtacgc ggacactttc ctggtcggca aaatgctgat gttgtttctc
     9301 tttcttcagc acgtttaaca cgctctggca ggcttcaatt ttttctggcg aaccttgtgc
     9361 ggtgttgata caggcgctgt atgcctcttt cagacgactg tcttcctgtg gcgcggttga
     9421 ttgcgcacaa gctaccagcc ccgatgccat tatggcgatg agaatcagtt ttttcatagc
     9481 aaattcctca aacgtgcggc aggcacaaaa cctgccgcgt cgggcatcag aagatggtga
     9541 atggtgcgat taccataaat ttcacgtcac gctcatcctg gaagatgttg ccgtaaccac
     9601 cgccccagct tgggatgtcg gagtggttgt cgtattcggt gaagtgcagt ttgaacatcg
     9661 tgcctttggc gcgaccgtcc tgaatggtgt agaccgcatc caggctgtat gcagactctt
     9721 caatagtccg gtttttgtcg tagtacgcat ccggattgct ctgctaggtc gcaggtttag
     9781 catcccatgc gtaaacgtag gaagcgccga tggcgaagcc tggaagattc cagtttttca
     9841 ggtcatacat cgcaccgaag aagaccgctt tttcgccgtt agcgttgaag tcggaacggt
     9901 tatcccacca gatatccagg cggccgttgg aggaagcgta ggttggagtc atacgttgca
     9961 ggaagtatcc ctgctgaccg tcagccttaa cccaggtgcc ttcgaggcgc aaatccacta
    10021 cgtcagccgc ccggtaacca aaggtcaacg cttgcagcca ggcggtgccg tcataaaggt
    10081 cgttgacgct gcgatcgtca actttatcgc gcgtaccata gaactggtag ctggtggtta
    10141 acgggctacc ggcgatatca aatttgtagc tggctttggc aaaatattga tcgatatacc
    10201 cttccgcctg accaaatgcc gcttccagta cgaagttatt tttgaagtcg tatttcgccc
    10261 caagggagtg cagataatca actttggtgg ttttatcgtt ctgataaaac tcatccattt
    10321 ccagatgcca cggcgctttg tattcgttgg tccacatgta ggagaaactc aacgcaccag
    10381 catcgccgta atcaaaattc gccccggctt ccgccccctg ataagtacct ggcataaagc
    10441 tccagtgcgg cgctaacagc gtttgaccag ttggctgaat gtaacctgcc ctcgcccaaa
    10501 ccggaccgta tttaaatttg gccgcagctt tatacaggct tataccgctt ttgtcgccgg
    10561 accagtcttc gtcataggct ttattacttt ttgaaaacgc gatttcgttc gggtgggagc
    10621 tgtcgccgtt ttccgccatt tcaatcgccg taaacgcagc aatatcaagg ccgaacatat
    10681 cagcagcata accggactga aaatcgaggt tggcattcca ggtggagtga gaaaggttgg
    10741 ttttgtattt gtcgccgtcg gtaacatctt tacggtcgcg ttcacgctgc cagtaatata
    10801 taccgccggt taaggttgaa tcgtcgatga atccttcggc gcgagcctcc ggcattgcca
    10861 taaagcccga cattgctgta acaccggcga tagccagcgc cagcgtacta cgtttgccac
    10921 taaacgtacg catgggttaa tcctctttga cgtataaatt gctgcaccaa aggtgaacag
    10981 cgaaaaaata aaaattaaaa aagtagcgtt gagaaaagtc tcgctgacta caggaacaga
    11041 ctatttttcg cgacgcgaat tatcaatctc tttctgtaac cagaatgtaa gaacatgagc
    11101 aaccgcacat attttgatga tttttgttac aaggcttaag tttgcggttg aaaggcttgc
    11161 agaagaataa aagcgtg
//
LOCUS       DAZOYL010000120        11061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZOYL010000120 DAZOYL010000000
VERSION     DAZOYL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.120"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1231
                     /gene="narU"
                     /locus_tag="ACHUOY_002308"
     CDS             <1..1231
                     /gene="narU"
                     /locus_tag="ACHUOY_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR0683073.1"
                     /translation="LAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIF
                     GGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFF
                     FPKAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANA
                     AWIWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGF
                     SAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIF
                     SALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGG
                     SDEQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIV
                     CVLLTWLVYGRRKFSQK"
     gene            1313..5053
                     /gene="narZ"
                     /locus_tag="ACHUOY_002309"
     CDS             1313..5053
                     /gene="narZ"
                     /locus_tag="ACHUOY_002309"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR0683074.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5050..6594
                     /gene="narH"
                     /locus_tag="ACHUOY_002310"
     CDS             5050..6594
                     /gene="narH"
                     /locus_tag="ACHUOY_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0683075.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6594..7289
                     /gene="narW"
                     /locus_tag="ACHUOY_002311"
     CDS             6594..7289
                     /gene="narW"
                     /locus_tag="ACHUOY_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0683076.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7286..7966
                     /gene="narI"
                     /locus_tag="ACHUOY_002312"
     CDS             7286..7966
                     /gene="narI"
                     /locus_tag="ACHUOY_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0683077.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8045..8938
                     /locus_tag="ACHUOY_002313"
     CDS             8045..8938
                     /locus_tag="ACHUOY_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR0683078.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9034..9879)
                     /gene="nhoA"
                     /locus_tag="ACHUOY_002314"
     CDS             complement(9034..9879)
                     /gene="nhoA"
                     /locus_tag="ACHUOY_002314"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR0683079.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10052..10621
                     /locus_tag="ACHUOY_002315"
     CDS             10052..10621
                     /locus_tag="ACHUOY_002315"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR0683080.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10625..10852)
                     /gene="pptA"
                     /locus_tag="ACHUOY_002316"
     CDS             complement(10625..10852)
                     /gene="pptA"
                     /locus_tag="ACHUOY_002316"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR0683081.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(10952..>11061)
                     /locus_tag="ACHUOY_002317"
     CDS             complement(10952..>11061)
                     /locus_tag="ACHUOY_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683082.1"
                     /translation="LTNDKVFKAGLRRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2571 a         2888 c         2965 g         2637 t
ORIGIN      
        1 tctagcagtt accgttaatc tcaataaaat cggttttaat tttactaccg atcaactctt
       61 tttattaacc gcattaccct ccgtttctgg cgcattattg cgtgttccct actcctttat
      121 ggtgcctata ttcggtggac gccgatggac ggtttttagt actgcaatcc tgattattcc
      181 ttgcgtctgg ctcggaattg ccgtgcaaaa tccgaatact ccttttggga tatttatcgt
      241 tatcgctttg ctatgcggtt ttgcaggtgc aaactttgct tcgagcatgg gcaatatcag
      301 tttcttcttt ccaaaagcca aacaagggag cgctcttggg attaatggcg gattaggaaa
      361 cttaggtgta agtgtaatgc agctggttgc accgctggtc atttttgtac ctgtatttgc
      421 ctttctcggc gtcaatggcg taccgcaggc cgacggttcg gtgatgtcgc tggcgaatgc
      481 cgcatggatt tgggtaccgc tactggcgat tgccacgatc gccgcctggt cagggatgaa
      541 tgatatcgcc agttcacgcg cctcaattga cgaccagctc cctgtcttac aacgcctgca
      601 tctctggctg ctgagcctgc tttaccttgc caccttcggt tcgtttatcg gtttttctgc
      661 gggttttgcc atgctggcaa aaacccagtt cccggatgtg aatattctgc gcctggcgtt
      721 ctttggccca tttatcggtg ccatcgcgcg gtcggttggt ggtgctattt ccgataaatt
      781 cggcggcgtg cgggtgacgt tgatcaactt tatttttatg gcgattttca gtgccctgct
      841 gttccttacc ttaccgggca caggctccgg taatttcatc gccttttacg ccgtatttat
      901 ggggctgttt ctgactgcgg gtctgggaag tggttctact ttccagatga tcgccgtcat
      961 ctttcgccag ataaccattt atcgggtgaa gatgaaaggc ggtagtgatg agcaagctca
     1021 gaaagaagcc atcaccgaaa cggcggcggc tctgggcttt atctcagcca ttggcgcagt
     1081 gggcggcttt tttattccgc aggcgtttgg catgtcgctc aatatgaccg gctctccggt
     1141 cggcgcgatg aaagtgtttt taatcttcta catcgtttgt gtgctgctga cctggctggt
     1201 ttatggtcgg cggaagttca gccaaaaata agagatcgat gcctgatgta caaccgtgtc
     1261 gcatccgaca aggcatcaga tcccaattca ttcctggagc aggagtcatg tcatgagtaa
     1321 acttttggat cgctttcgct acttcaaaca aaagggcgaa acctttgccg atggtcacgg
     1381 acaggtgatg catagcaacc gcgactggga ggacagctat cgccagcgtt ggcagttcga
     1441 caaaattgtg cgttccaccc acggtgttaa ctgtacaggc tcctgtagct ggaaaatcta
     1501 cgttaaaaat ggtctggtga cctgggaaat ccaacagacc gactacccgc gcactcgccc
     1561 tgaccagccc aatcatgaac ctcgcggctg cccgcgtggc gcaagttact cctggtatct
     1621 ctacagcgct aaccgcctga aatacccgct cattcgtaaa cgactgattg aactgtggcg
     1681 tgaagctctc aagcaacaca gcgatccggt actggcgtgg gcatcgatta tgaacgatcc
     1741 gcaaaagtgc ttaagctaca aacaagtgcg tgggcgcggc gggtttatcc gctccaactg
     1801 gcaggaactt aaccaactga ttgccgccgc taacgtctgg accatcaaaa tctatggtcc
     1861 ggatcgcgtt gccggtttct cgccgatccc ggcgatgtcg atggtgtcat acgctgccgg
     1921 aacgcgttat ctgtcgctgt taggcggcac ctgtttaagc ttctacgact ggtattgcga
     1981 cctgccgccc gcctcgccga tgacctgggg cgaacaaacc gacgtgccgg aatctgccga
     2041 ctggtataac tccagttaca tcatcgcctg ggggtctaac gtaccgcaga cacgtacgcc
     2101 ggacgcccac ttctttaccg aagtacgtta caaaggcacc aaaactatcg ccattacccc
     2161 tgactactct gaagtggcca aattgtgcga ccagtggctg gcaccgaaac aaggcactga
     2221 tagcgccctg gcgatggcaa tgggccatgt gattttaaaa gagtttcatc tcgataatcc
     2281 cagcgactac tttatcaact actgccgccg ctatagcgac atgccgatgc tggtaatgct
     2341 ggaacctcgc gacgatggta gctacgtacc cgggcgcatg atccgcgcgt ctgacctggt
     2401 agatggacta ggcgaaagca ataatccgca gtggaaaacc gtggcggtta ataccgcagg
     2461 tgaattggta gtgccgaatg gttcgattgg tttccgctgg ggagaaaaag gcaaatggaa
     2521 tctggaatcc attgccgccg gtaaggaaac cgaattgtcg ttaaccctgc tcggtcaaca
     2581 tgacgctgtt gcaggcgtgg ccttccccta ctttggcggc attgaaaatc cgcattttcg
     2641 cagcgtaaaa cacaatccgg tgctggtgcg ccaattgccc gttaaaaacc tgacgttagt
     2701 cgatggcaac acctgtccgg tggtcagcgt ttatgatttg gtgctggcga attacggcct
     2761 cgatcgcggg ctggaagatg aaaacagcgc gaaagattac gctgaaatca aaccgtacac
     2821 cccagcctgg ggtgagcaaa ttaccggcgt accgcgccag tatattgaaa ccatcgcccg
     2881 cgaatttgcc gataccgccc ataaaacgca tggacgctca atgattatcc tcggcgctgg
     2941 cgtcaaccac tggtatcaca tggatatgaa ctaccgtggg atgatcaata tgctgatctt
     3001 ctgcggttgt gtcgggcaaa gcggcggcgg ctgggcgcac tatgtcggtc aggaaaaact
     3061 gcgcccacaa accggctggt tgccgctggc ctttgcgctc gactggaacc gcccaccgcg
     3121 ccagatgaac agtacctcgt ttttctacaa tcattccagc caatggcgct atgaaaaagt
     3181 ctctgctcag gagttacttt caccgcttgc cgatgccagt aagtacagcg gtcatctgat
     3241 tgatttcaac gttcgcgccg aacgcatggg ctggctacct tctgcgccgc agctggggcg
     3301 taacccgctc gggattaaag ctgaagccga caaggccgga ttatccccca cagaatttac
     3361 cgcccaggcg ctgaaatcgg gcgatttacg tatggcctgc gaacaaccag atagcggcag
     3421 caatcatccg cgtaatttgt ttgtctggcg ctctaacctg cttggctcct ccggcaaagg
     3481 ccacgagtat atgcagaagt atctgctggg gaccgaaagc gggattcagg gcgaggaact
     3541 cggtgccagc gacgggatca aaccggaaga agtcgagtgg caaactgcgg cgattgaagg
     3601 caagctcgac ctgctggtga cgctcgactt ccgcatgtcc agtacctgcc tgttctccga
     3661 tatcgttctg cccaccgcca cctggtacga aaaagacgat atgaacacct cggatatgca
     3721 tccgtttatt catccgcttt ctgcggcggt cgatccggcg tgggaatcac gcagcgactg
     3781 ggaaatctac aaaggtatcg ccaaagcatt ttcgcaagtg tgcgtgggtc atcttggcaa
     3841 agaaaccgac gtggtattac aacccctact gcatgactct ccggcagagc tctcacagcc
     3901 gtgtgaagtg ctcgactggc gcaaaggcga atgcgatctg attccaggca aaaccgcgcc
     3961 gaatattgtg gcggtggagc gcgactaccc tgctacgtat gaacgcttta cctcgctcgg
     4021 gccattgatg gacaaacttg gcaatggtgg taaagggatt tcgtggaata cccaggatga
     4081 aatcgatttc ctcggcaaac tcaattacac caagcgtgat ggcccggcac aggggcgtcc
     4141 gctgattgac accgccattg acgcttcaga agtgattctg gcactggcac cggaaaccaa
     4201 cggtcatgtt gcagtcaaag cgtggcaggc gctgggcgag atcaccgggc gcgaacatac
     4261 ccatctggcg ctgcacaaag aggacgagaa gattcgcttt cgcgatattc aggcgcagcc
     4321 gcgtaaaatt atctccagcc ccacatggtc cggtctggaa agcgatcacg tctcctataa
     4381 tgcgggatac accaacgttc atgagttaat tccgtggcgc acgctgtcgg gacgccagca
     4441 gctctatcag gatcatccgt ggatgcgtgc ttttggtgaa agcctggtgg cttatcgccc
     4501 gcctatcgac acccgtagcg tcagtgagat gcgccagatc ccgccaaacg gcttcccgga
     4561 aaaagcactt aacttcctga cgccgcacca gaaatggggc attcactcaa cctacagtga
     4621 aaacctgcta atgctgacgc tctctcgcgg tggaccgatt gtctggatca gcgaaaccga
     4681 tgcccgagaa ctgaccattg tcgataacga ctgggtggaa gtctttaacg ccaacggcgc
     4741 gctgacggct cgtgcggtgg tcagccaacg tgtaccgccg ggtatgacca tgatgtatca
     4801 cgcccaggaa cgcattatga atattcctgg ttcggaagta actggcatgc gcggcggcat
     4861 tcataactcg gtcacccgtg tttgcccgaa accgacgcat atgattggcg gttacgcgca
     4921 gctggcctgg ggctttaact actacggcac cgtcggctcg aaccgcgacg aattcatcat
     4981 gatccgcaag atgaagaacg ttaactggct ggatgatgaa ggtcgcgatc aggtacagga
     5041 ggcgaaaaaa tgaaaatacg ttcgcaagtc ggcatggtgc ttaacctcga caaatgtatc
     5101 ggttgccata cctgttcggt gacctgtaaa aacgtctgga ccggacgcga aggcatggag
     5161 tacgcatggt ttaacaacgt cgaaaccaaa ccgggcattg gttatccgaa aaactgggaa
     5221 gatcaggaag agtggcaagg cggctgggtc cgcgatgtga atggcaagat acgcccgcgt
     5281 ctgggcagca agatgggcgt aataaccaaa atcttcgcca acccggtggt gccgcagatt
     5341 gatgattact acgaaccttt caccttcgac tacgaacatt tgcatagcgc accggaaggc
     5401 aaacatatcc ctactgctcg cccgcgttcg ctgattgacg gtaagcggat ggacaaagtg
     5461 atctgggggc caaactggga agaactgctg ggcggcgagt ttgaaaaacg tgcccgcgac
     5521 cgcaacttcg aggccatgca aaaggagatg tacgggcagt ttaaaaacac cttcatgatg
     5581 tacctgccgc gcctgtgcga acactgcctc aatcccagtt gcgtggcgac ctgcccaagc
     5641 ggcgctatct acaaacgcga agaagacggc attgtgctga ttgatcagga taaatgccgt
     5701 ggctggcgtt tgtgcattag cggttgtccg tacaaaaaaa tctacttcaa ctggaaaagc
     5761 ggcaagtcag aaaaatgtat cttctgttat ccgcgaattg agtcaggcca acccaccgtg
     5821 tgctcagaaa cctgcgtggg tcgcatccgg tatctgggcg tgctgcttta cgacgccgac
     5881 cgcattgagg aagcggcgag caccgagcgc gaagttgacc tctatgaacg tcagtgcgaa
     5941 gtgttcctcg atccacacga tccctcagtg atcgaggaag ccctgaaaca aggtattcca
     6001 caaaacgtga ttgaagctgc ccagcgttcg cctgtctaca aaatggcgat ggactggaaa
     6061 ctggcgctac cgctgcaccc tgaatatcgc accctgccaa tggtctggta cgttcctccg
     6121 ctgtcgccga ttcagtccta cgcagatgcg ggcggtttgc cgaaaagcga aggcgtgctg
     6181 cccgccatcg aaagcctgcg tattccggtg caatatctcg ccaatatgtt gagtgccggc
     6241 gataccggtc cggtactgcg ggcgctgaaa cggatgatgg cgatgcgcca ctatatgcgt
     6301 tcacaaaccg tggaaggcgt tactgatact cgtgccatcg acgaagtagg cctgagcgtc
     6361 gcccaggtcg aagaaatgta tcgctatctc gccattgcca actatgaaga tcgttttgtt
     6421 atcccgacca gccatcggga aatggcgggc gatgccttcg cagaacgcaa cggctgcggt
     6481 tttacctttg gcgacggttg ccacggttcg gacagtaaat tcaacctgtt caacagtagc
     6541 cgtatcgatg ccatcaacat caccgaagtg cgcgacaaag cggagggcga ataatgcaga
     6601 tcctcaaagt gatcggcctg ttgatggagt atccggatga gctgttgtgg gaatgcaagg
     6661 aggacgcgct ggcgttgatc cgccgcgacg cgccgatgct tacggatttc acccacaacc
     6721 tgcttaacgc gccgctgctg gataaacagg ccgaatggtg cgaagtgttt gaccgcgggc
     6781 gcaccacgtc gctgctgctg ttcgaacatg ttcatgccga gtcccgcgat cgcggccagg
     6841 caatggtgga cctgctggcg gagtatgaaa aggtcggcct gcagctggat tgtcgggaac
     6901 tgcccgatta tctaccgctg tatctggagt atttaagcgt actgcctgac gatcaggcga
     6961 aagaaggatt gctcaatgtt gcgccgatcc tcgccctgct tggcggtcgc ttaaaacaac
     7021 gcgaggcacc gtggtacgcg ttgtttgatg ctctgctgca actggcggga agtatacttt
     7081 caagtgacag tgtcacaaaa caagtaaaca gcgaagagcg cgatgacacc cgccaggcgc
     7141 ttgatgcagt gtgggaagag gaacaggtga agtttattga agataacgcc acggcgtgtg
     7201 acagctcgcc gcttaatcaa tatcagcgac gctttagcca ggatgtcgcg ccgcagtatg
     7261 tcgacatcag tgcgggaggt gggaaatgat tcagtatctg aacgtctttt tttacgatat
     7321 ctacccgtac atttgtgcga cggtgttttt cctcggcagc tggctgcgct acgactacgg
     7381 gcagtacacc tggcgcgcct cctcaagtca aatgctcgat aaacgcggga tggtgatatg
     7441 gtcgaatctg ttccatatcg gcattttggg gattttcttc gggcacttgt tcggcatgtt
     7501 aacgccgcac tggatgtacg cgtggttttt gccagtggca gcgaaacaac tcatggcgat
     7561 ggtgctcggc ggtatttgcg gtgtgttgac gttgattggc ggcgcaggac tgctgtggcg
     7621 cagtctgaca aatcagcgcg tgcgtgccac ttccactacg ccggatatca tcatcatgag
     7681 cattttgctg atccagtgtc tgttggggct aagtaccata ccgttttcag cacagtatcc
     7741 tgatggtagc gaaatgatga agctggtggg ttgggcgcaa agcattgtca ctttccgtgg
     7801 tggatcgtca gagatgctca acggcgtagc gttcgtcttc cgcctgcatc tggtgttggg
     7861 aatgaccatc ttcctgctct tcccgttcac ccgactggtg catgtgtgga gcgcgccgtt
     7921 tgagtacttt actcgtcgat atcaaattgt gcgttcgcgg cgataatttc accctggccg
     7981 gatagccctt cgctctccgg ccacttgcag gctatgatag ttctccagtc acaggagaac
     8041 gttaatgaaa ccgcaggtgt accacgtcga tgcttttacc tctcaaccat ttcgcggcaa
     8101 ttctgccggt gttgttttcc ccgccgataa tcttagcgaa gcacaaatgc agcttatcgc
     8161 ccgcgagtta ggccattcgg aaactgcttt tctgctgcac agcgacgaca gcgatgtgcg
     8221 catccgctac tttacgccaa cggttgaagt gccgatttgc ggtcacgcga cagtagctgc
     8281 gcactatgta cgtgcgaagg tgttaggttt aggaaattgc acgatctggc aaacatcgct
     8341 ggcaggaaaa catcgcgtga ctatcgaaaa gcacaacgat gattatcgta tttcgctgga
     8401 acaaggtacg ccgggctttg aactgccgct ggagggtgaa gtacgcgcgg cgattatcaa
     8461 cgcattgcat cttactgaag acgatattct gccgggtttg ccgatccagg tcgcgacgac
     8521 cgggcattct aaagtgatga tcccattgaa gccggaagtg gatatcgacg ctctttcgcc
     8581 tgaccttaat gcgctgaccg ctatcagtaa acagattggc tgcaatggtt tcttcccgtt
     8641 ccagatccgt ccaggcaaaa acgaaaccga tggtcgtatg ttctcgcctg cgattggtat
     8701 tgtggaagat ccggtgactg gcaatgccaa tggcccgatg ggtgcatggt tggtacatca
     8761 caacgtattg ccccacgatg gcaacgtgtt gtgtgttaaa ggccatcagg ggcgcgcatt
     8821 ggggcgtgac ggcatgattg aagtgacggt gacaattcgt gataaccaac cggaaaaagt
     8881 caccatttca ggcacagcgg tgattttgtt tcatgccgaa tgggcaattg aaatctgaaa
     8941 tcgttgcctg atgcgcttcg cctgtcaggt ctacaaccgg gctaatgtat gccggataag
     9001 gcgtttacgc cgcatccggc aacctgacat aaattatttt cccgcctccg gatgcgtatc
     9061 aaacgccgcc atcaccagcg ctaactcatc cacagtaaag ccatgtttcg catcatccac
     9121 gcccagacca aactgttcct gcatcacggc atataacgac gccacatccg gtagatttcg
     9181 ctgctccacc gcgtgcccat tttcataatg ggtaaaatga aaattggtca gtgtcagctt
     9241 gccgccgtcc ggcaaatggc ggcacatcag caaatgatgg cgaaaatgcg actgcggcca
     9301 gtgcgccgac cagaaattgc ccatcacata atcgctttgt tgctgctcgc agagatcgaa
     9361 gcggtacatc ggctgccaat gctgatgatg attaaactgc aacacccaat catcaccctc
     9421 ctgcaacaac cgatactctc cgtgtggcgt ggtctgcacg agatcggaaa ctaaacgaat
     9481 cggcgcggtt agcgtctgcc caccgaaacc gacatcagca atccattttt ccccttccag
     9541 ttccaccaac agcaaacgat gggtgcgcgg cggtaatgct ggcggatttg ataacactac
     9601 gcgccctaac aagctgcgaa cgttaaaccc cagctcgcgt aacacccgct caaacacgcc
     9661 attctgctca aaacagtaac cgccacgacg ggctatcacc agtttctctt ccagcgattg
     9721 atcatcaagc tgtatttccc tcggcagcaa aacgtcaagg ttttcaaacg gaatggtgca
     9781 attgtgtttc aggtgcaatg cacgaagcgt atcaatattg accgcagcag ctcccgacca
     9841 gttaatacgg gcaaaatagt gattcagaat gggcgtcatc aggcgtttcc cttagtggtt
     9901 ttctcaactt ataacctgga atagacgccg caccggctat tttgtgcgat cgcggacatg
     9961 catcacgttt gcaaatcgcc aatcccgtta atttcgttac ttattgcgtt acggactacg
    10021 cttaaaaaaa ccttcttcgc gaggcgatac catgagccga ttcatcccca tagaattaca
    10081 ccatgccagc cgtctgttaa atcatggccc aactgtcttg atcaccagtt tcgatgagca
    10141 atcccagcgg cgtaacatta tggctgcagc ctggtcaatg ccggtggagt ttgaacctcc
    10201 acgcgtggca attgtggtag ataaatcgac atggaccagg gagttgattg aacgtaacgg
    10261 taagtttggc atcgttatcc cgggcgttgc agcaactaac tggacgtggg cggtgggaag
    10321 tgtgtcggtg cgtgaagaag ataaatttaa ttgctatggc attccggtgg tgaaaggtcc
    10381 ggtgcttggt ttgccacttg ttgaagagaa atgcctggcg tggatggagt gccgcctgct
    10441 acctgcgact tctgcgcaac aaaaatacga cacgctgttt ggcgaggtgg tatcagcagc
    10501 ggcagacgca cgggtatttg tcgaaggccg ctggcagttt gatgatgata agctcaatac
    10561 gttgcatcat ttaggtgctg gcacgtttgt tactagcggc aagcgtgtta cggcaggtta
    10621 agcattacat gctgtaaccg ggtttcttta tcaaagcctc catttggggc tcgatttcgg
    10681 catcccagat agcttgccaa gattctggtt gaatctgctg tagagcaatg cttatcgagc
    10741 tatctttact gttcagatgg cgaataataa cgtcggtaat atctgcagca agtgctgctt
    10801 tttgttgttc gtccagttca cgcggaaagc atttaatgtc gatgtgcggc atgattacgc
    10861 tccttttgaa agatgtcagt aaaattaaca cacattgtga atgtcatgtg tccggtaaag
    10921 cgtttatacg acaataatgc ccagggcaag attacgaaag cccgctcccc gtaaggactg
    10981 acgccaggta gtttctgccc atggctgctt ttcgcatctt acgtcttaac cctgccttga
    11041 ataccttatc attcgtcaaa a
//
LOCUS       DAZOYL010000121        11058 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZOYL010000121 DAZOYL010000000
VERSION     DAZOYL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.121"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            125..1330
                     /gene="gltS"
                     /locus_tag="ACHUOY_002318"
     CDS             125..1330
                     /gene="gltS"
                     /locus_tag="ACHUOY_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR0683083.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(1499..3580)
                     /gene="recG"
                     /locus_tag="ACHUOY_002319"
     CDS             complement(1499..3580)
                     /gene="recG"
                     /locus_tag="ACHUOY_002319"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR0683084.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(3586..4275)
                     /gene="trmH"
                     /locus_tag="ACHUOY_002320"
     CDS             complement(3586..4275)
                     /gene="trmH"
                     /locus_tag="ACHUOY_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR0683085.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(4282..6390)
                     /gene="spoT"
                     /locus_tag="ACHUOY_002321"
     CDS             complement(4282..6390)
                     /gene="spoT"
                     /locus_tag="ACHUOY_002321"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR0683086.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(6409..6684)
                     /gene="rpoZ"
                     /locus_tag="ACHUOY_002322"
     CDS             complement(6409..6684)
                     /gene="rpoZ"
                     /locus_tag="ACHUOY_002322"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR0683087.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(6739..7362)
                     /gene="gmk"
                     /locus_tag="ACHUOY_002323"
     CDS             complement(6739..7362)
                     /gene="gmk"
                     /locus_tag="ACHUOY_002323"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR0683088.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            7620..9302
                     /gene="ligB"
                     /locus_tag="ACHUOY_002324"
     CDS             7620..9302
                     /gene="ligB"
                     /locus_tag="ACHUOY_002324"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR0683089.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(9299..9916)
                     /locus_tag="ACHUOY_002325"
     CDS             complement(9299..9916)
                     /locus_tag="ACHUOY_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR0683090.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10208..11032)
                     /gene="dinD"
                     /locus_tag="ACHUOY_002326"
     CDS             complement(10208..11032)
                     /gene="dinD"
                     /locus_tag="ACHUOY_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR0683091.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
BASE COUNT         2441 a         2805 c         3014 g         2798 t
ORIGIN      
        1 cattaacagc tgagaatttg acccaggaaa gcataactcg atacccgtca tagttcatga
       61 agttgccact gcgcgatgaa gtatgacgag tatgaaagag tgatgcggat acaaaggagt
      121 aactatgttt catctcgata ctttagcaac gcttgttgcc gcaacgctga cgttgctgct
      181 cgggcgtaag ttggtccatt ccgtctcctt tttgaagaaa tacaccatac cggaacctgt
      241 tgcgggtggt ttgttggtgg cgctggcgct actagtactg aaaaaaagca tgggctggga
      301 agtcaacttt gatatgtccc tgcgcgatcc gttaatgctg gctttcttcg ccaccattgg
      361 cctgaacgcc aacattgcca gtttgcgtgc cggtgggcgt gtggttggca tcttcttgat
      421 tgtggttgtt ggtctgttgg tgatgcaaaa tgccattggc attggtatgg ctagcctgtt
      481 agggcttgat ccgctgatgg ggctgttggc cggttctatt acgctttccg gcggtcacgg
      541 tacgggcgct gcgtggagta aattgttcat tgaacgttat ggcttcacca atgcgacgga
      601 agtggcgatg gcctgtgcaa cgttcggtct ggtgctgggc ggcttgattg gcggtccggt
      661 ggcgcgctat ctggtgaaac actccaccac gccgaacggt attccggatg accaggaagt
      721 cccgacggcg tttgaaaagc cggatgtggg acgcatgatc acctcgttgg tgctgattga
      781 aactatcgcg ctgattgcta tctgcctgac ggtggggaaa attgttgcgc aacttttggc
      841 tggcactgct tttgaactgc cgaccttcgt ctgtgtactg tttgttggcg tgattctgag
      901 caacggtctg tcaataatgg gcttttaccg cgtctttgag cgtgcggtat ccgtgctggg
      961 taacgtaagc ttgtcgttgt tcctggcgat ggcgttgatg gggctgaaac tgtgggagct
     1021 ggcttcgctg gcgctgccga tgctggcgat tctggtggta cagaccatct tcatggcgtt
     1081 gtatgccatc ttcgttacct ggcgcatgat gggcaaaaac tacgatgcgg cagtgctggc
     1141 tgcaggtcac tgtggttttg gcctcggtgc aacgccaacg gcaatcgcca acatgcaggc
     1201 gattactgaa cgctttggcc cgtcgcacat ggcgtttttg gtggtgccga tggtcggtgc
     1261 gttctttatc gatatcgtca atgcgctggt aattaagttg tatttgatgt tgccgatttt
     1321 tgccggttaa ccgatgaagc ggcggtagaa gtgccgccgc aacaaagaca aatgcctgat
     1381 acgcttcgct tatcaagcct gcgtattgat tcataattta ttgaatttgt agggtgatga
     1441 atcgcatccg gcaggaaggt agggtaacct gaaatggcgg tcttctcact gccgcctttt
     1501 acgcattcga gtaacgttcc gtctccggca tccagcgttc tatcagggct tttgcctgtt
     1561 gtgggtaacg ttcgtgaata tggcgtgcca ggcgctgaac ttccgggatc atcgcctgat
     1621 cgcgcagtaa atccgccact ttaaattcag cattacccgt ctgacgcgtg cctaacaatt
     1681 cgccagggcc gcgaatctcc agatcttttt gcgcaatcac aaaaccgtcg ttactgtcgc
     1741 gcagcacttg caggcgaatt tgcgccgttt tagaaagcgg cgttttgtag agtagcacgc
     1801 agtgagaagc caccgcgcca cgacctacgc gcccgcgcag ctggtgtaac tgcgccagac
     1861 ccagacgctc agggttttcg ataatcatca ggctggcgtt aggcacatca acaccgactt
     1921 caataacggt agtggcaacc agcagatgta gctcaccttg tttaaacgac gccatcaccg
     1981 cctgtttctc ggctggtttc atccgcccgt gtaccaggcc aacattcaat tctggtagcg
     2041 ccagtttcaa ctcttcccag gtagcttccg ccgcctgcgc ttccagtaac tctgactctt
     2101 caatcaacgt acaaacccag tacgcctgac gaccttcagt catgcaggcg tggcgcacgc
     2161 ggtcaatgat gtcggtacgg cgagtatcag gaatagcgac cgtagtcact ggcgtgcggc
     2221 ctggcggcag ttcatctatc accgaggtat cgagatcggc atacgccgtc attgccagcg
     2281 tgcgggggat cggcgtggcg gtcatgatca actgatgcgg atggaagcct tgctgctggc
     2341 ctttctccca taacgccaga cgctgatgca cgccaaagcg atgttgttcg tcgataatca
     2401 ccagcgccag accgttaaac tgcacctgtt cctggaagat ggcgtgcgta ccaacaatca
     2461 tctgcacctg accgctggcg atggcttcct gctgagacag tcgcgctttg cctttctgct
     2521 taccggcgag ccagcccact tcgataccga gcggttcaaa ccagttgcgg aagttattgg
     2581 cgtgctgttc ggcgagtaat tcggttggtg ccatcaatgc cacctgtttg ccgtgggcaa
     2641 tcgcacgcag cgcggcgagg gcggcgacca gcgttttacc ggaacctaca tcgccctgta
     2701 ccaggcgcat catcggcaca tccagcgcca tatcgcgctc gatctccgcc actacgcgtg
     2761 cctgtgcgcc cgttggcttg aacggtaagg ctgcgaggag tttatttttc agcgcatcat
     2821 tggcgctcag cggctgggca tgaaaacgct gtgctccggc acgtaaggct aacatgctga
     2881 ggttgtgcgc cagcagttct tccagaatca gacgacgttg cgccggatgc tgcccggttt
     2941 ccagatcgct aagctgtagc gtcggtggcg ggcggtgcaa agtgcgcaac gcttccggta
     3001 gcgtcatcat tccttgtgac agttccggcg gcaggagttc ttcaatggcg caggtgtcga
     3061 gcagatccag cgcctggtcg gttaatttac gcagcgtggc ctgctttacg ccttccgttg
     3121 ttggataaac cggcgtgagc gtttcctgta attctggcgt gctgagatcg ccctgcacgc
     3181 ggtattccgg gtggatcatc tccgcaccat atttaccgcg ctttgcttcg ccataagcca
     3241 gtacacggcg gcccgtcgcc aggctatttt tcattgccgc gctgaaattg aaaaagcgca
     3301 tggtgaggat gccggaaccg tcgctgatct ggcaggtcat catccgccga ccgccgaagg
     3361 agatattgca gttcagcact tcgccttcca ccgtggcata aacgcccggc agtagttctc
     3421 cgatggggta gagatgggtg cgatcttcgt agcgcagggg aaggtgtaag agtagatcct
     3481 gcacggtatg caggttgatt ttcgccagtt tgttactaag tgctgcgcca acgcccgtta
     3541 gggaactgag tgggacagca tctaacaggc gacctttcat ggcacttacc ctgcagcctg
     3601 catagtagcc caccagtcgg catcagcttc gatctcgcct tgctgattga cgtggggata
     3661 aggcaggcct ttgcgttttg cgactttcgc cagcaccgga tagccgcctt caaacaacag
     3721 gcgttgttgc tctgcttccg gcaacatgct gttttcacgc aggtacatgc ctgcattttg
     3781 ccgctgacgc tgggcttcgt aaagaatgag ggctgaggca acggaaacat tcagcgactg
     3841 caccatgccg atcatcggaa tgatgatgtc ctgatccgcc agggccaatg cttcctgcgt
     3901 gatgcccgtt ttctcctgtc ccatcaaaat gcaggtcggg cgagtgtaat caatttcgcg
     3961 gaaatcgaca gcgttatcag aaagatgggt tgccagaatc tgcataccct ggcctttcag
     4021 atgagcgacg gcatcgccaa tggtgcggtg tgttttcacc tgtacccagc tgttactacc
     4081 cgccgctgcc gaagccatgg tgcgcatgcg gctaccaggc cagacggcgt gaacttcatg
     4141 tacgccaacg gcatctgcgg tacgaataat cgcagaaacg ttatgaggtt tgtggacctg
     4201 ctccatgcag acggtcagat caggctgccg cctggcgagc atttcgcaga tgcgtgcata
     4261 acgtgttggg ttcataaaac attaatttcg gtttcgggtg actttaatca cgtctggcat
     4321 cacgcggatt ttgcgcatga tattcgccag atgcacacgg tcacgggcgg tcagacgaat
     4381 aaaggcgctg tagacgcgac catctttctc ttccgtattc aaactttgaa tattcgaagt
     4441 cgtggtgtta attgccgccg tcaggtttgc cagcgcgccc tgatgattga acatctccac
     4501 cttgatttcg gtgatgaact cctgcgccgt ctctttatcc cattccacag ccataaactt
     4561 ctctggctct ttctggtagc cacggatatt acggcaggat tcatggtgga tcaccagacc
     4621 tttaccgggg ctgacgtggg cgataatcgg gtcgccagga atagggcggc agcatttcgc
     4681 aaaggtgatc agcacgccat cggcaccttt aatgggcaga tgtccgtggc tttgggttgc
     4741 cggtggaatg gaggcgtccc catgttgcag atttttcgcg accaccacgc tcattgcgtt
     4801 accaagtccg atttctgcca gcagatcgtc aagcgttgcc agcttcatgc gatccagctc
     4861 gcgctgaata ttttcctgcg ggatttcatt cagcttacgg ctaccaccca aagcatggtt
     4921 gagcagacga cggcccaggc ttacagaatc atcacgcttg aggtttttca gcaactgacg
     4981 aattttggcg cgcgcttttg agctaacgac aaagttcagc cacgcggcat tcgggcgagc
     5041 gcccggtgcg gtaatgattt caacggtttg accgctggta agcggctgcg acagcgggta
     5101 aggctggcgg tcaacgcgtg cgcccacgca ggcatgaccg atatcggtat gcactgcata
     5161 agcgaagtcg acgggcgttg caccggcagg cagctcgaca attcgccctt ccggtgtgaa
     5221 aacgtaaatc tcatccggga agagatcgga tttaacgctc tcgataaatt caaacgaact
     5281 accggcgctc tgttgcagct ccagcaggct ttgcatccag cgctgggcgc ggatttgcgc
     5341 ggtggtgctg gtttcgccgt gctctttata agcccagtgc gcggcaacac ccatctccgc
     5401 catctgatcc atatcttcag tacggatctg gacctcaacc ggcacaccgt gcgggccgat
     5461 catcgaggtg tgcaaagact gatagccgtt cgcttttgga atggcgatgt agtctttcac
     5521 gcgacccgga cgcggcttgt acaggctatg catttgcccg agcacgcgat aacaggtgtc
     5581 ggaatcattg acgatcacgc ggaaagcgta gatgtccatg attgagtgaa aacgctgctc
     5641 tttgagcacc attttgcagt aaatcgaata aagatgcttt tcgcgaccac tgacgcggca
     5701 cggtattccc gcttcctgca aacgcccttc gatttcagaa agaatcttct ggatcatctc
     5761 tttacggttg ccgcgcgcgg ctttcaccac ttctttgatt acgcgataac ggttgggata
     5821 cagcgcctca aaacccagct cttcgagttc ggttttaatg tggtggatac ctaaacggtg
     5881 cgccagcggg ctgtaaattt cgagagtttc acgggcgatg cggcgacgtt tgtccgggcg
     5941 aagtgagccc agcgtgcgca tgttgtgggt acggtcggca agtttgatga ggatgacgcg
     6001 gatatcctgc accatcgcca taatcatctt gcgaaagttt tcggcctgcg cctctttctt
     6061 atcgcggaac ttgagtttat caagtttcga taccccctct accagctcgg cgacgctttt
     6121 accaaaaagc tgttccatat cctggtaggt ggcgggtgta tcttcaatca cgtcatgcag
     6181 cagcgccgcc atcagcgttt catagtcgag tttcatctcg gccagaatgc aggcaaccgc
     6241 taccgggtgc gtgatgtagg gttcaccgct tgaacgtgtt tgcccctcgt gagcatcacg
     6301 tgcaacgaga tacgcctgcc gcagacgctt gatttggtct tccggcaggt aggtttgaat
     6361 cagttgattc aggctttcaa acagatacaa gggcgacccg ctttgtgatt aacgacgacc
     6421 ttcagcaata gcggtaacgg cttgtaattc agcggcttcc tgctcttgct gttcctggcg
     6481 ttcgcgaacg tcgaggatct ggttgttgat cagaccttct tcgatttcgc gcagcgcgat
     6541 tacagtggtt ttatcgtttt cttccggtac cagcggatcc tttccgccta cctgcatctg
     6601 acgagcgcga cgcgcggcga ccagtaccag gtcaaaacgg ttaccaattt tctctacagc
     6661 gtcctgaaca gttacgcgtg ccatacttaa aaagctccac aggtgaagaa atgactgggc
     6721 atgatactga aatcaggttc agtctgccaa caatttgctg attaaagcgt catgacgctg
     6781 cttttggcgg ctcatgcgca gacgttcggc gcgaataatg gtcttcaaat cggtcaacgc
     6841 ggtatcgaag tcatcattca caatcagata atcatattcg gcgtaatggc tcatttctgc
     6901 aacagcttgc gccatacgct ttgcaatgac ctcttcgctg tcctgaccgc gaccgcgtag
     6961 acggcggtcc agttcaattt tggacggcgg taaaataaag atactccgcg cgtgcggcat
     7021 cttctggcga atttgctgcg cgccctgcca gtcgatatcg agaaaaacat cgacaccggt
     7081 cgccagtact tgctcaatgg cctcacgcga agtgccatag taattaccaa aaacttctgc
     7141 gtgttcgagg aacgcatctc tgctaatcat ttctttaaat tcatcatgat taacaaagaa
     7201 ataatgttca ccgtggactt caccaggacg cggttggcgt gtggtgtgtg aaacagaaac
     7261 ctgggtgtca tacaacggtt gggtttttaa taaagcctga atcaggctgg atttacccgc
     7321 accactgggg gcagaaacaa tataaagcgt gccttgagcc atgagtatct ttcgtatgtg
     7381 attagcgaaa taaagcctac atacgagctt attatacacg gcgcggccat gtgacgtagc
     7441 ccttgctcac actttttgcg caaagttgtt gcgttttgcg tagctgcttc cgcatttgcc
     7501 tgtcattcat tgcaataaag acgattttca ggaagcgtcc tcgcagactc cgtttttgcc
     7561 actcggcttt gctttcatca cgttgtatat ctgcctgact atctacagcg gaggtgatga
     7621 tgaaagtatg gatggcgata ttaataagta tcttgtgctg gcaatcatct gtgtgggcgg
     7681 tctgtccggc ctggtcgcca gccagagcac aggaagaaat ttcccgcctg caacagcaaa
     7741 taaaacagtg ggacgatgac tactggaagg aagggaaaag tgaggtggaa gacggtgttt
     7801 acgatcagtt aagcgcccgt cttacgcagt ggcaacgctg ctttgggagc gagccccgcg
     7861 acgtgatgat gccgccttta aatggtgcgg ttatgcatcc ggttgcccat acgggtgtac
     7921 gcaaaatggt ggataaaaac gcattaagtc tgtggatgcg agaacgtagc gatctttggg
     7981 tgcagccaaa agttgatggc gttgcggtaa ccctggttta tcgggacggg aaactgaaca
     8041 aagcaatcag tcgcggtaat ggcctgaaag gcgaggactg gacgcagaaa gttagcttaa
     8101 tttccgctgt gccgcaaacc gttagcgggc ctttagccaa cagtacgctt cagggggaaa
     8161 tatttctcca gcgcgagggg catatccaac aacaaatggg gggaataaat gcccgcgcaa
     8221 aagttgctgg cctgatgatg cgccagggga atagcgacac gctaaactca ctggctgttt
     8281 ttgtctgggc atggccggat ggaccgcagt taatgaccga tcgtttaaag gagctggcta
     8341 ccgcaggttt tactctaacg cagaggtata cccgtgcggt taaaaatgct gatgaaattg
     8401 cgcgcgtacg caatgagtgg tggaaagcga aattaccctt cgtcaccgat ggcgtggttg
     8461 tgcgagcggc taaggaaccc gaatcccgcc attggctacc aggccaggca gaatggttgg
     8521 tggcctggaa atatcaacct gtagctcagg ttgccgaagt gaaggcgatt cagtttgcgg
     8581 tgggtaagag cggtaaaata tcggtggttg cgtcactcgc acctgtcatg ctggatgata
     8641 aaaaagtcca gcgggtgaat attggttccg tcaggcgctg gcaggagtgg gatattgcgc
     8701 ctggtgatca gatcctcgtc agccttgccg gtcagggtat tcctcgcatt gatgatgtgg
     8761 tctggcgcgg tgcagaacgt acaaaaccga caccaccaga aaaccgcttt aactcgttga
     8821 cctgctactt tgcttctgat gtttgtcagg aacagttcat ttcacgctta gtctggctgg
     8881 ggtcaaaaca ggttcttggg ctggatggca ttggtgaggc cggctggcgc gcgctgcatc
     8941 agactcatcg ctttgagcat atcttttcct ggcttttatt aacgccagag caattacaga
     9001 acacgccggg gatcgcgaaa agtaaaagtg cgcagttatg gcatcagttt aatctggctc
     9061 gtaagcagcc ttttactcgc tgggtgatgg caatgggaat accgttaacc cgggcggcgc
     9121 ttaatgccag tgatgaacgg tcctggtcgc aacttttatt tagcacggag cagttctggc
     9181 agcggctgcc ggggactgga tctgggcgcg ccagacaggt tattgaatgg aaggaaaatg
     9241 cgcaaatcaa gaagctgggc agttggctgg ctgcccagca gatcacaggt tttgaacctt
     9301 agtgaccttc gtggctgtag taaaagaccg gtaatcccag cttaagacgt agcgccagta
     9361 agcgggcaaa aaagccgaat accagggtgg agataatcac cacatcatgg ttagaaacat
     9421 agtgttgcag cgcgatgtac agcacggcgg aggcaaatga gacaccggca tacaactctt
     9481 tctggaatac cagtgggatg cgtttacaga acatatcgcg caaaacgccg ccaaacacgc
     9541 cggtggttac cgccgcgaca acggcaataa ttgggccgtg ccccatatcc agtgcaacct
     9601 gtgcgccgat gatagaaaag accaccagtc cgagcgcatc gagcaccaga aacactttgc
     9661 gcaggtaagg cattacagga gcaacgatag tggtgagtac tgcggcggta gcgacgataa
     9721 tcacatattc cgggtgtttg acccaaccga gcggatagtg gcccagcaga atatcgcgca
     9781 ctgaccctcc gccaattgcg gtggcggtcg caataataat tacgccaaat gtatccatgc
     9841 gccgtcgtcc ggccgccagc gcccccgtca tggcttcggc agtaatgcca accaaataca
     9901 aaatgtgtaa cagcatggtt ctctccctaa aaatctgtct ggcagggtag cgatttgtgc
     9961 gtattgtcac gattgagatt ttttaaggtg agaggaaatt gataacttgt agttatgtga
    10021 aatcgctatt ttctgtagtg tagatgcatt cttctgactt cttgtctgag aaaatgcata
    10081 agaaaaaata ggttaaaatt gaggggtgga aaagaaaggt gcaatgttta gccattgtgt
    10141 tcactgcacc tggactttgg cttacgcatc gggtatctca attaagaaaa agagttttta
    10201 gtttctgcta tttcttctct gtaattttta cactgttttc caactgattg atgctgacct
    10261 gcggggtcgg caactcctca ggcatggttc cgccaagttc ctgaatggtt tgcctcacct
    10321 tgctacccac gtcaaagtgt gtggtattag cttgctgttt tgaatttacg ccatcccgct
    10381 tgagtttttc ttctgtttgg gtagctcgaa agagattagc cgccagttct gttgaaccca
    10441 tatgatccag gatcttctga ctctttttca gccccttccg ctgatggatg gctttctgat
    10501 ctaatccacc atacagcccc cggtaaccat gattctgaaa tatggcgaaa tcgatagctg
    10561 tcgctacccc agcttgctgc gctgcctcaa ctaattgttt gttatgttct ttcaactcat
    10621 ttctcagaaa aagacgcttt tcgtcttcac gaagttgcct gaaagcttca tcgtcagcca
    10681 gctcctgccg tcgggtctgg atagcaaaat aagtttgccc tgccgcaata accggtttcg
    10741 cagggtcgcc gttttgtact accaggtaac aggcatagcg agagagatga acatcttcaa
    10801 gttctcgttg agcccctgaa cctaatacaa ccattttggt ggtttcaacg aaatggtcag
    10861 atgcagcttg attgctggct tcgcacgctt gagtcgcgcg ggccagtacc ttttgaaaat
    10921 tgcgccagtc ccgataatcc agtaacggtg ccaattctct cgctgaccaa aattcttgcc
    10981 cttcagtgcc ataatgccgt atttcttcaa aaggttgatg atgttcgttc attgcccact
    11041 ccatgtgata tcgattgc
//
LOCUS       DAZOYL010000122        11026 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZOYL010000122 DAZOYL010000000
VERSION     DAZOYL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.122"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..210
                     /locus_tag="ACHUOY_002327"
     CDS             <1..210
                     /locus_tag="ACHUOY_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR0683092.1"
                     /translation="KIVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMF
                     AQTTKGVLETGIYRGVVSIYLSQDI"
     gene            302..2986
                     /locus_tag="ACHUOY_002328"
     CDS             302..2986
                     /locus_tag="ACHUOY_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR0683093.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2983..4071
                     /locus_tag="ACHUOY_002329"
     CDS             2983..4071
                     /locus_tag="ACHUOY_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR0683094.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4154..5451)
                     /gene="exuT"
                     /locus_tag="ACHUOY_002330"
                     /pseudo
     CDS             complement(4154..5451)
                     /gene="exuT"
                     /locus_tag="ACHUOY_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5933..7345
                     /gene="uxaC"
                     /locus_tag="ACHUOY_002331"
                     /pseudo
     CDS             5933..7345
                     /gene="uxaC"
                     /locus_tag="ACHUOY_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            7360..8847
                     /gene="uxaA"
                     /locus_tag="ACHUOY_002332"
     CDS             7360..8847
                     /gene="uxaA"
                     /locus_tag="ACHUOY_002332"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR0683095.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8930..9481
                     /locus_tag="ACHUOY_002333"
     CDS             8930..9481
                     /locus_tag="ACHUOY_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR0683096.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9486..10730)
                     /gene="sstT"
                     /locus_tag="ACHUOY_002334"
     CDS             complement(9486..10730)
                     /gene="sstT"
                     /locus_tag="ACHUOY_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR0683097.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2916 a         2672 c         2857 g         2581 t
ORIGIN      
        1 aagattgttc cgctgacagt aacctggggc ggtgaagaaa ttaaagctga tgcggcaacc
       61 acttttaccg caaccaaaat ctttgcttcc gatgcgttaa ctaacggttc tctggctaaa
      121 aacctgatgt ttgctcaaac caccaaaggt gttctggaga caggcatcta ccgtggtgta
      181 gtaagcattt atctgtctca ggatatctaa tccagataac agaattaaca cgtaacaaag
      241 ggaggggtag ccctccctta ttttaactgt tttagtattt gttatggatt tcaggattgc
      301 catggataaa aaattactgg ctcttttgat cctggcgagt ctcagcccgg cagaggcgac
      361 attaaccgaa attcccgcag ggtttgaggt tattgctcag ggacagcagg agtatatcga
      421 ggtttatttt tcagggaaaa gtctcggtaa atattatgca atggttaatc ttgataccgt
      481 aacatttctt gatccagcaa gtctatataa caagctggaa ctggatgtag acgatcagaa
      541 aatcgcgcat atagtgaaag aaaaattatc gcagccgcta gctcaccacg gtgaattggc
      601 ttgcggttat gtacgtactg actcagggtg tggttttctg aataccgata cgctggaaat
      661 aatctataat gatgaagaaa gttcggcaac gttgtttatt aatccgcaat ggaattcagc
      721 tttcgatgcg aagtcattat atttaaatcc agacaaaaat actgttaatg cttttataca
      781 tcagcaagac atcaatgttc tggcacagga tgattaccaa tcgttgtcta ttcagggaaa
      841 cggtgcgctg ggaataacag aaaatagtta tattggtgca cactggaatt tcaacggtta
      901 tgatgcagat gatgtcagtg acagtaatgc tgacgtcagc gatctctatt atcgttatga
      961 ttttttacgt cgttattatg tgcaggcggg tcgcatggac aaccgcacac tatttaatgc
     1021 acaaggcggg aactttacct ttaactttct gccactcggt gcaattgatg ggatgcgtat
     1081 cgggtcgaca ctgagctatt taaaccaggc gcaaagccag caaggaaccc cagtaatggt
     1141 tctgctttcg cgcaattctc gtgttgacgc ttatcgtaat gagcaacttt tgggatcgtt
     1201 ttatctcaat agtggttcgc aatttattga taccagttcc tttccgccag gcagctacag
     1261 cgtggcatta aaagtctacg aaaataacca actcacccgc accgagcttg tgccgtttac
     1321 caaaaccggc ggtctgactg acggaaatgc gcaatggttc ttacaggcag gtaaaactac
     1381 atcacaggtt tctgatgatg aaagttcagc ttatcagcta ggagtacgcc tgccattaca
     1441 tccgcaatat gagctctacg cagggctggc gaatgccgat gatgtgagtg ctttcgagtt
     1501 aggtaataac tggacggcag atttaggcgg ggtggggaat cttgcaatca gcgccagcgt
     1561 gttccgtaac gatgacggcg gcaaaggtga tatgcaacag gccaactgga gtaatccggg
     1621 atggccgacg ttgggctttt atcggaccaa ctctgacggc gatgcttgta caaccgacag
     1681 cagagagagc tataacgcct taagctgtta tgaaagtatt tccgcgacgg tttcacagaa
     1741 ttttgtcggc tggaatatga tgctgggtta tacccgcaca caaaataaca ctgatgatag
     1801 tttgcgttgg gataaacagc agagctttga aaataactat cttcgccaga caacagcgca
     1861 aagtatctct gaaactgtac aacttagcgc ttcccgcgct tttgtgatgc gtgactggat
     1921 tttgagtacg tcagtcggtg ttttccatcg taatgacaac ggtggcgata acgacgacaa
     1981 cggcttgtac ttatcgtttt cgttatctga cacgccaacg atggatagca ataacaacag
     2041 ccattcaaca aatgtgtcta cggattatcg ttatagcgaa caggatggcg atcaaacgtc
     2101 atggcagtta tcgcatacct tttataacga ttcattcagc cataaagaac ttggcgtaac
     2161 cgtcggaggc ctgaacaccg ataccataaa cagcgcggtt aacgggcgtt gggatgggca
     2221 atacggaaat gtctacgcta ccgtatctga tagttatgac cgtaagaatc atgatcatct
     2281 ttcggccttt acgggcactt atagctctac gctggctgtc agttgctatg gcgttaattt
     2341 gggagccagt ggtacagacg atttgctggg tgcggtactg gtggatgtga aaggcttctc
     2401 tgaacaggat gaagagagtc aggatctgca actcgaagcg cgggtggcag gcagccgaac
     2461 tttgcagctt ggtcaaagtg acagtgtgtt gttcccttat cctggatttc agtctggttt
     2521 tgttgaggtt aacgacagta gccagggcaa tcagcagggg acaacaaaca tcattaacgg
     2581 tgcagggaat cgtgaattaa tgttgttgcc tggcaagctg cgctatcgcg aagtgtctgc
     2641 cagctttaac tacaactata tcggtcgttt attactgccg gcagcggtga aaaaattccc
     2701 gattgtgggg ctgaacagcg ccatgttact ggtagctgaa gatggaggat ttacacttga
     2761 aattaacggc agcgaaaaag agctctatct gctttccggg cagcaattcc ttaagtgtcc
     2821 actgagtgtt gtaaagaaac gcgccagcat tcgttacagc ggagatgtta cttgtagtgt
     2881 ggtgacttat tcacaattac cggagtcgat tcaggttcag gcacagttga aacagcctaa
     2941 attacgtgga aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt
     3001 gcggcgatat tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc
     3061 agtgaaatta cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa
     3121 gcgtcggcag gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa
     3181 acctgtacag ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt
     3241 gggatgcgtt ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa
     3301 gcgccgccag gatgcaaggg gggcaaaggg cttcagacga atcttaagga ttctaataga
     3361 tcttcatgta cagaagatgg tcaacattat tatatatacg atacaaagtt tcttacgctc
     3421 taccttgagc agacagagat gaagaatttg ccgattggtg gcgtctggaa ggggaaagtt
     3481 aaattacatt cgaacagccc ggcccaggac tatttcgcaa atattaccct gaatacgctc
     3541 gaccccaacc atattgacgt gttcttcccg gagttcgccc acgccacgcc aagggtgcag
     3601 ttagacttgc atccaacagg aagcgttaac ggcagcaact acgcgcaaga tctgaccatg
     3661 ttggacatgt gcctgtacga tggttttaac ggtaatgcca tcagttatga aatcatgctc
     3721 aaagatgaag ggcgaccagc tgcagggcgc agagacggtg acttctctat ctatcgtcaa
     3781 ggagggacca ccaccgacga gggagaacgc attgattacc gggtcaaaat gtacaacccg
     3841 gaaaccggtg ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa cagcattaac
     3901 ctgaaacgtg tgcgtccggt ggtactgccc ggtattcgct atgccgtgat gtgtgtgccg
     3961 acacccttga ctctggcagt cgataaattc agcgtgatgg ataaacaggc cgggtactac
     4021 atgggcaaat tgtcagtaat ctttacgcct tccttgccaa ccatcaattg accccgcagg
     4081 ccagaaaaaa ctaaacaacg aagccgcctg ataaggcggc ttcgtcatta caacttacgt
     4141 taaacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
     4201 tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg
     4261 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     4321 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     4381 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
     4441 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
     4501 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
     4561 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     4621 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     4681 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcca
     4741 ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     4801 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     4861 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     4921 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga
     4981 ttgccatacc accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta
     5041 gcctactgcg atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc
     5101 cgggatcatc gctgcttccg cggcaccgac cgcaccacga gcaacagcca ggccacccca
     5161 gctacctgcc agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata
     5221 accgattttc gtacccagca catccagtac ataacctgct accggttgca tgaccgtata
     5281 agcagcagaa taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc
     5341 catcagagtt ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc
     5401 gagcgtcacc agtgcgatca tataccaacg taacccttta attttacgca tgtaaaacct
     5461 catcgttttg ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt
     5521 agttacgacc gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc
     5581 gtgctaattc ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca
     5641 atttgcattc cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacttt
     5701 aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg
     5761 aggccagtac tggcgcggcc tgcagcgaga tttatcactt tgagagtaat tttttaacta
     5821 cgtttattga tctaactcac gaaaatatct tcggactctg gaaattggtg tgataacttt
     5881 gtcagcatcg caccataagc aagctagctc actcgttgag aggaagacga aaatgactcc
     5941 gtttatgact gaagatttcc tgttagatac cgaatttgcc cgccgtctgt atcacgacta
     6001 cgcaaaagac cagccgattt tcgattacca ttgccatttg ccgccgcagc agattgcgga
     6061 agactatcgt ttttaaaacc tgtatgacat ctggctgaaa ggcgatcact acaaatggcg
     6121 cgctatgcgt accaacggtg tggccgagcg tctgtgtacc ggtgatgcat ctgaccgtga
     6181 aaaatttgac gcctgggcgg cgactgttcc gcacactatc ggcaacccgt tataccactg
     6241 gacgcacctt gaactgcgtc gtccgtttgg tatcactggc aaattgcttt ctccgtcaac
     6301 tgccgatgaa atctggaacg aatgtaacga actgctggcg caggataact tctccgcgcg
     6361 cggcatcatg cagcagatga acgtgaaaat ggtcggcacc accgatgacc cgatcgattc
     6421 tctggagcat cacgcagaga tcgccaaaga cggctctttc accatcaaag tgctgccgag
     6481 ctggcgtccg gacaaagcct tcaacatcga acaggcgacc tttaacgact acatggcgaa
     6541 gctgggcgaa gtttccgata ccgacattcg ccgctttgct gacctgcaaa ctgccttgac
     6601 caaacgtctg gatcacttcg ccgctcacgg ctgtaaagtg tctgaccatg cgctggacgt
     6661 ggtgatgttt gctgaagcga acgaagcgga actggacagc attctggcgc gccgtctggc
     6721 tggcgaaacc ctgagcgagc acgaagtggc acagttcaaa actgcggtac tggtgttcct
     6781 tggtgccgaa tatgcacgtc gcggctgggt acagcagtac cacattggcg cgctgcgtaa
     6841 taacaacctg cgtcagttca aactgctggg gccggatgta ggctttgact ccatcaacga
     6901 ccgtccgatg gcggaagagt tgtctaagct gctgagcaag cagaacgaag aaaacctgct
     6961 gccgaaaacc attctctact gcctgaaccc gcgcgataac gaagtgctgg gcaccatgat
     7021 cggtaacttc tagggcgaag gtatgccggg caaaatgcag ttcggttccg gctggtggtt
     7081 taacgatcag aaagacggta tggaacgtca gatgacccaa ctggcgcagc tcggtctgct
     7141 gagccgcttt gtcggtatgc tgactgacag ccgtagcttc ctgtcataca cccgtcacga
     7201 atacttccgc cgcattctgt gccagatgat cggtcgctgg gtggaagcgg gcgaagcacc
     7261 ggcggacatc aacctgctgg gcgagatggt gaaaaatatt tgctttaaca atgcgcgtga
     7321 ctacttcgcc attgaactga actaaggtct ggggttgata tgcaatacat caagatccat
     7381 gcgctggata acgtcgcggt cgctttagca gatttggctg aaggcacaga agtcagtgtc
     7441 gataaccaga ctgttacgct gcgccaggat gttgctcgtg gacataaatt tgcgttaacg
     7501 gatatcgcaa aaggggccaa cgtcatcaaa tatggcctgc cgattggtta tgcattggcg
     7561 gatattgcgg cgggggaaca cgtccacgcc cacaatacgc gcacgaatct gagcgatctg
     7621 gatcagtatc gctatcaacc tgattttcag gatctgcctg cgcaagcggc agatcgtgaa
     7681 gtgcagatct atcgtcgcgc taacggcgat gtcggggtgc gtaacgagct gtggatcctg
     7741 ccaaccgtgg gctgtgtcaa cggcatcgcg cggcaaatcc agaaccgttt cctgaaagag
     7801 accaacaacg ccgaaggtac cgacggcgtg ttcctcttca gccacaccta cggctgctca
     7861 caactgggcg acgatcacat caacatccgc accatgctgc aaaacatggt gcgccacccg
     7921 aacgcgggcg cagtgctggt gattggtctg ggctgtgaaa acaaccaggt tgccgcattc
     7981 cgtgaaacgt tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg ccaacagcag
     8041 gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc tgtataacgt gatgcgcaac
     8101 gataaacgcg agccaggcaa actcagcgaa ctgaagtttg gtctggagtg cggtggttct
     8161 gacggtcttt ctggtattac tgctaacccg atgctggggc gtttctctga ctacgtgatt
     8221 gctaacggcg gtactaccgt actgaccgaa gtgccggaga tgtttggcgc agagcagttg
     8281 ctgatggacc attgccgcga cgaagcaacg tttgaaaaac tggtcaccat ggtcaacgac
     8341 tttaaacagt actttattgc ccatgatcag ccgatctacg aaaacccatc accggggaac
     8401 aaagcgggcg gtatcaccac gctggaagac aaatcacttg gctgtaccca gaaagcgggt
     8461 tccagtgtcg tggttgacgt gctgcgttac ggcgagcgtc tgaaaacgcc tgggctgaac
     8521 ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg ccctggcggg tgcgggctgc
     8581 catatggtgc tgttcagtac tggtcgcggc acgccatacg gtggatttgt gccgacggtg
     8641 aaaatcgcca ccaacagtga actggcggcg aagaaaaaac actggatcga ctttgacgcg
     8701 ggtcagctga tccacggtaa agcgatgccg cagttgctgg aagaatttat cgacaccatc
     8761 gttgagtttg ccaacggtaa gcaaacttgc aacgagcgta acgacttccg cgaactggcg
     8821 atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt tgttaataaa aaacggcgtt
     8881 tcataatgtg aagcgccgtt tgcttttcca tacctgataa ggatttatca tgaccgcgta
     8941 ttggctggcc cagggcgtgg gtgtcatcgc ctttctgatt ggtatcacaa catttttcaa
     9001 tcgtgacgaa cgtcgcttca aaaagcagct ttcggtctat agcgccgtta ttggcgtaca
     9061 tttttttctt ctgggcacct atcccgctgg tgccagtgcc atccttaatg ccattcgtac
     9121 attgattacc ttacgcacgc gcagcttatg ggtaatggcg atttttattg tgctgactgg
     9181 cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta ttgccggtta tcggtacgat
     9241 tgtcagtacc tgggcgctgt tccgctgtaa agggctcacc atgcgctgcg tgatgtggtt
     9301 ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg tcgataggcg gcacaatgat
     9361 tgagggtagt tttctgctta tgaatggctt gaatatcatt cgtttctggc ggatgcagaa
     9421 aaggggaatt gatccgttta aagtagagaa aacccctccc gccgtagacg aaaggggtta
     9481 aacaattaat tacgcagggc gctatttgcc agacgatcgt cttccgcctg gcaagctgcc
     9541 gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt cgcaagagtc ctgcaatacg
     9601 ccgatgataa agccgacggc aaccacctgc atggcgatat cgttcgaaat accgaacata
     9661 ttacaggcca gcgggatcag cagcagagaa ccccccgcca cgccggatgc gccacaggca
     9721 cacagagaag ccaccacgct caacagcagc gccgtgggca gatcgaccgg gatacccagc
     9781 gtattaaccg cagccagcgt caacacggta atggtgattg ctgcgcccgc catattgatg
     9841 gtggcaccca gcggaataga aacggaatag gtatcgcgat ccagattcag cttttcacac
     9901 agtgccatat tcaccggaat gttcgccgca gagctgcggg tgaagaaggc atacacgccg
     9961 ctttcgcgca ggcacagcag caccagcggg aacgggttac gacgaatttt ccaccacacc
    10021 agcaatgggt taaccaccag cgccaccagt aacatacagc caaccagcac gaccagcagt
    10081 tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca gggtagaaga aaccagacca
    10141 aaaataccaa tcggtgcgaa gcgaatgacc agtttcacca taaaggtgac ggcattcgac
    10201 atatcgttga ccaggttttt ggtggtctcg ttaccgtgac gcagtgcgaa accgaggcca
    10261 attgcccaca ccaggatccc gatgtagtta cctttcagca gcgcgtcgat ggggttggaa
    10321 accatgctca ttaccagccc gcgcatcact tctacaatgc ctgacggtgg cgaaatatca
    10381 cccgcgctac tggataagtg cagggtagaa gggaaggcga agctgaaaac gactgcggcc
    10441 agagcagcag agaaggtacc cagcagatag aggaacaaaa tagggcggat attggttttc
    10501 tgcccatgct ggtggttagc aatagatgcc atcaccagca tcaacaccag gatgggggca
    10561 acggctttca gtgcgccgac gaacaaagta cctaacagac caacagcttc cgccgcgggt
    10621 tttgatatcc atgccagaag aatccccaga acaaggccga ccaggatttg ttttaccagg
    10681 ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt gcgtagtcat ttttcgatcc
    10741 tttcattgtg ttatcgcaca tccctggtgc gttctgtcga cgcatctttc aggatgtaac
    10801 aaagtgtttg caaggtcgag tataaggaaa gttacagctt cgggaagcga aaaatgctgg
    10861 attttaaaga gaagtcatat tttgaaactt aatggttaca tttgtttgac ataaacagag
    10921 ggaaggactg gctactaagg ctgattagat aacatatggg caaaactatt tggaatatga
    10981 gaacggttgc gctcctttaa tgtgctatgc catataaagt cactgg
//
LOCUS       DAZOYL010000123        11003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZOYL010000123 DAZOYL010000000
VERSION     DAZOYL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.123"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..280)
                     /locus_tag="ACHUOY_002335"
     CDS             complement(<1..280)
                     /locus_tag="ACHUOY_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0683098.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(447..1187)
                     /locus_tag="ACHUOY_002336"
     CDS             complement(447..1187)
                     /locus_tag="ACHUOY_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR0683099.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1280..1915)
                     /gene="argO"
                     /locus_tag="ACHUOY_002337"
     CDS             complement(1280..1915)
                     /gene="argO"
                     /locus_tag="ACHUOY_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR0683100.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="ACHUOY_002338"
     CDS             complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="ACHUOY_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR0683101.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            3110..3208
                     /locus_tag="ACHUOY_002339"
                     /pseudo
     CDS             3110..3208
                     /locus_tag="ACHUOY_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="ACHUOY_002340"
     CDS             complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="ACHUOY_002340"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR0683102.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="ACHUOY_002341"
     CDS             complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="ACHUOY_002341"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR0683103.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5779..6798)
                     /gene="epd"
                     /locus_tag="ACHUOY_002342"
     CDS             complement(5779..6798)
                     /gene="epd"
                     /locus_tag="ACHUOY_002342"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR0683104.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7170..7601
                     /locus_tag="ACHUOY_002343"
     CDS             7170..7601
                     /locus_tag="ACHUOY_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR0683105.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7624..8202
                     /locus_tag="ACHUOY_002344"
     CDS             7624..8202
                     /locus_tag="ACHUOY_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR0683106.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8203..8910
                     /locus_tag="ACHUOY_002345"
     CDS             8203..8910
                     /locus_tag="ACHUOY_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR0683107.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RQAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8898..9575
                     /locus_tag="ACHUOY_002346"
     CDS             8898..9575
                     /locus_tag="ACHUOY_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0683108.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9569..10246
                     /locus_tag="ACHUOY_002347"
     CDS             9569..10246
                     /locus_tag="ACHUOY_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0683109.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10218..10931)
                     /locus_tag="ACHUOY_002348"
     CDS             complement(10218..10931)
                     /locus_tag="ACHUOY_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR0683110.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10928..>11003)
                     /locus_tag="ACHUOY_002349"
     CDS             complement(10928..>11003)
                     /locus_tag="ACHUOY_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0683111.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2627 a         2920 c         2740 g         2716 t
ORIGIN      
        1 ctcgtgaacg acgccctgag ttatggattt cattatcaat cgcagaaagt cgttgataaa
       61 ccgggaacag tttttgatac agttcctgac cggctttatt gaggctgata ttattatctt
      121 tgcgggtaaa tagcgtatag ccaatttgtt cttccagcgc ggcaatgctt ttaccaaatg
      181 gcgaagccgt catatggatt ttttctgccg cccgggcaat attattggtt tgcgctaata
      241 aaatgaaatt gcgcatcttt ttcgaaataa aaatgtccat cccccctccc cgcatccttg
      301 tccgcttaac cgtttcagtc agcctatcct tgatgaaacc gcgagcaaag ataggtgatt
      361 acgtcatggt tttacagaaa attacagaaa aaggaggcaa tatcgggtaa aggcattagc
      421 ccgacgaata cgtcgggcta caagtattat tgtgctgcag gtgttttagc gggttgttga
      481 tccacaggtt ctaactggaa gaccacatcg acctgatcat caaactgaat agcggcctgc
      541 tcgtaagttt cctgggcaga caccggcgcg gcatcggctt tcatcatccg caccattggg
      601 ctgggctgat agttggaaac atggtagcgc acgctatata ccggccccag tttacgatga
      661 aagccgttcg ccagttcctg cgcctgatga atcgcgttat caatcgctgc cttacgcgct
      721 ttgtctttat aggcatccgg ctgcgccacg cccagcgaca cagaacgaat ttcgttaaga
      781 cccgccttca gcgcgccatc cagcaaggaa ttcagtttgt ctaactgacg gagcgtgact
      841 tccaccgttc tcacagcgcg gtagcctttc aggatacttt taccatcctg ataatcataa
      901 tctggctggg tgcgtaagtt cgctgagctg atatcttttt tcgcgatctg attgagttca
      961 aggaaggaaa tgtattgtgc gacgcgctca tctgcctgtt tcttggctgt agcggcatcc
     1021 ttcgcggcca cgttaacttc aatcgcaaga gtggcaatgt ctggcaccgc atccacactt
     1081 gccgtaccgg aggtgacaat atgcggtcca tccggcaatt cgttagcctg cgctgccatc
     1141 ccgctaatac ccattaatgc cgccagggcg ataactttga acttcacgtt agtctctccg
     1201 tgttaattga cttgcccaaa attcaggacc tctggaggca agcttagcgc ctctgtttta
     1261 tttttccatc agatagtgcc taactgaaca aggcttgtgc atgagcaata ccgtctctcg
     1321 ccagctgcaa ggctataaac cacatgacac atcccactac cagattgata atgcgctgtg
     1381 attttgccgt gcgcaggcgc ggtgccagcc aggctgcgag aatagccaga ccaaagaacc
     1441 acaggaaaga ggcgctaatt gtcccgagcg caaaccaacg ttttggttcg acgtcaagct
     1501 gcccgccaag gctgcccagt acaacaaaag tatccaggta aacatgcgga ttcagccagg
     1561 tcactgccag catggtggcg ataattttcc atctgccttg cttcaggact tcggcactag
     1621 ctaactcaat attactactc atcgctgttt taaaagcgcc aaaaccatac cacagcaaga
     1681 aggctacgcc gccccaggtg accagcgcca gcaaccacgg cgactgcatc aataacgcgc
     1741 tgccaccaaa aatcccggcg caaatcagga ccaaatcgct gatagcacaa agtaaggcaa
     1801 tcataatgtg gtactgacga cgaatgccct gattcatcac aaaagcattt tgtggaccga
     1861 gcggcagaat catagctgcc ccaagagcaa taccttgaaa gtaataagaa aacacgttgg
     1921 atattccgaa ttaagtatct gatgcagaga ctatatcgcg ggattgttat tagcggaaat
     1981 tgataatatt aattggcaat cagaataact aataagagag aggccagaga gtattatctg
     2041 gcctcagcgt tgattacgca gctttgtctt ctttcacccg cttaaagttc acatccattt
     2101 gcgggtacgg gaagctgata ccggcggcat caaattcacg tttaatacgc tccagcacat
     2161 cccagtacac gttttgcaga tcgccgctgt tgctccagac gcggaccacg aaattaatcg
     2221 acgatgcacc aagttcgttc agacgcacag tcatttcgcg atctttcaaa atgcgatctt
     2281 cagactggat aatattggtc aggatctgct taacctgatc gatatcggaa tcatacgcca
     2341 cgccaataat aaattcgtta cggcgaactg gctcgcgaga gaagttaata atatttccgg
     2401 caataatttt accgttcgga ataacgataa ttttaccgtc tgcagtacgc atggtggtgg
     2461 agaaaatctg cacactcagc acagtaccgg ctacgccgcc gagatcaaca tattctccgg
     2521 cacggaacgg gcggaacatg acaagtaaca cgccagcggc caggttagaa agtgacccct
     2581 gcaaagccag accaacagct aagcctgcgg caccgagtac agcaatgact gacgctgttt
     2641 gtacacccac gcgtcccagg gcagcgatta gcgtaaaggc gataataccg taacggacta
     2701 atgcagaaag aaaatcagca acagtggcat cgattttacg ggagatcatc aggcgattca
     2761 ccgcgttgga aatcatccgc gcgataatca aaccaacgat gatgatcgcg agtgccgcca
     2821 cgatgtttac tgcataactt agcagcagcg cctggttagc taccagccag cttcccgcgc
     2881 cgtttatgct atcgacaaca ttcaaatctt ccattcaata ttccttttca actgactcaa
     2941 aatggagaaa tacactccgc cgccttatga cgggcagtct gacaagggta aacaataaag
     3001 cataattttg ccaaatagat cacagatttc tcatctgaat caccttgctt ttggcgaaaa
     3061 aatccccgca gagcggggct cagacagata tcaatgtgcg ctttgtcatg ccggatgcgg
     3121 cgtgaacgcc ttatccggcc tacaaaaacc tgcaaattca atatattgca ggatacgtgt
     3181 aggcctgata agcgtagcgc atcaggcaat tttgcttttg gcgaaaaaaa gacccgcaga
     3241 gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg ttcagttcct
     3301 ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc catacgcgcg
     3361 gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccaac tgcccctgca
     3421 gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc cattgggtat
     3481 cggtatcgat gttcattttt actacgccgt agcttacgga gtctttgatt tcctgagcag
     3541 tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg ttgtgtttct
     3601 tggaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg ttacccggct
     3661 tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg ctgattttgc
     3721 tcagttcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca gaagcgtcca
     3781 tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg atttccagag
     3841 tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg ttctcttgca
     3901 gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg gtagctgcga
     3961 agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc ttcgcgcagt
     4021 ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga acgtgatgcg
     4081 caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc acgcctttac
     4141 cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct ttaactttag
     4201 cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact gctggcagtg
     4261 cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca gtgattacgc
     4321 caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt cgggccttat
     4381 actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact
     4441 tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa ttatttagag
     4501 tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc ctgccctgtg
     4561 attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac tttaccttcc
     4621 acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc agcaatgccg
     4681 aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc ttcgctgtct
     4741 gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc gaacacgcca
     4801 accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag ttcctgagcg
     4861 gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac agatttcagg
     4921 gtagccgatg cagtttcaga gaactcggtt gctacgcgaa catcagacgg aaccgggatg
     4981 ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta cagggattta
     5041 cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc aacaatcagc
     5101 tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac tttagaacca
     5161 ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag cgcgtccagt
     5221 tcagctgcca acagcgggcc tgcgcacgca acatcagcga atttaccgat accgtgagta
     5281 gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc acacagtgca
     5341 gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa gcgaacgttt
     5401 tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc tttaaccaga
     5461 cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag agagaattct
     5521 tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac tttcgcgcct
     5581 tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc gctggttact
     5641 ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt cccagcaaga
     5701 tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc taaaagtttt
     5761 gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg tcgtgtcgag
     5821 cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga tcaggtgtgc
     5881 gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt ggttaaaatc
     5941 tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac cttgtgctgc
     6001 tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg tcacgcttaa
     6061 atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa agcgatcgtt
     6121 aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg gaatgatcga
     6181 ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa cctgttgatc
     6241 gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg catcatctaa
     6301 caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa cgatgcggtg
     6361 ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga gatcgttact
     6421 gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt cgccatgctc
     6481 gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt cacgccaggg
     6541 gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa caaaaagttg
     6601 atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat atttcaacaa
     6661 atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa tttccgcccg
     6721 gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga agccatttat
     6781 cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt tgccagacag
     6841 agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac tgattggtta
     6901 attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac gtggaataaa
     6961 aggaatgatt gtccagtcaa agaaaacaat tatctgactt acgtcacgtt ttcagccccg
     7021 cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa atcttttcgt
     7081 tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc gatattatcc
     7141 gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca tattcttccc
     7201 ccgcacgctt ttatgcactg cgcctcctcc ccgggcagga agttttttcc cagctacacg
     7261 cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc ggtagcctga
     7321 ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctccttaac ggtacattcg
     7381 aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat ctctgcgttt
     7441 ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc gtgcggacca
     7501 cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa ccttgcgcgc
     7561 tttctggtta cgacgagttg catatttccc ccgttaaata actactttca aagaggtttt
     7621 cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat atctatcgcc
     7681 atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt tctcgactcc
     7741 atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct gaccggctta
     7801 ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt tgcacccgtc
     7861 gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt tcgcaccctg
     7921 ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt cgctgtaccg
     7981 ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt cgttgcctgg
     8041 atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacagtgat aggcgcgaac
     8101 ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca gctgccgatt
     8161 cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc atttacctca
     8221 ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca gaccattttg
     8281 ccgatctata gcgcggcgac ctttttttgt cttattgcgc taaaggccac gcggcggcag
     8341 gcaaaatatg tcgcctggct gatgttctcg ctgggagccg ggctgtggct ggtacacggt
     8401 ggctggctaa cagaatggct aagcggcacg ccgcgttcgc cggagcgttg gacgcatgct
     8461 attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg gatgcagtac
     8521 gtccccgtgc aatgttttat ccgcgctctg tttgcttctc gcctgccgcc aggcgtcgct
     8581 tatctgtttg ccggaccgtt attggtcgtt gagcagttaa aacggcaatt agcgattatt
     8641 catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca gcgtttacgt
     8701 gcgatgcccg cgttaattat tctgctaaca cacaatgcgc ttaacgatct ggctgtccgt
     8761 ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac gttgtgggca
     8821 ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct gatgctaaca
     8881 gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg acacactcaa
     8941 cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc gccatctttg
     9001 gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg tatccggact
     9061 ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt gggcatctcc
     9121 cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat ttgcagctct
     9181 ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat ctgtgtcttg
     9241 ctgaaacaga gattatggcg cggattgatg ccgccctgac tctgaccgag tgccagccgt
     9301 tgcgccatcg tcacccggca acactctccg gtggagagac acaacgcgtg gtcatcgcct
     9361 gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagt cgcctgacgc
     9421 cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa cgtggttcgc
     9481 tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag caagcgtggc
     9541 aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat cttatcgctg
     9601 gcctagcgca gcaacggatt gtctttgcga catttctctg caactcaaac aaggtgaatg
     9661 gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag taatggcagg
     9721 tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga aaaacctgaa
     9781 aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa accaactttt
     9841 tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat gcccggcgga
     9901 tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag acactgccaa
     9961 ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg cctgcctgga
    10021 agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg acgaaaattg
    10081 gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga gcgtggtagc
    10141 aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc gactggaaga
    10201 tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt catcattaag
    10261 gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc gtcggtacgg
    10321 tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg ccccaccaga
    10381 cgctcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca aaattgcgca
    10441 agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat gacgagtggt
    10501 gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta ctgcggccac
    10561 gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac gtcaaatgtc
    10621 tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt gtaatggtga
    10681 aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg cacgagatat
    10741 tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag aaaaaccacc
    10801 gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa cggtttatgc
    10861 acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt caccgttaat
    10921 tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc agcgccgttt
    10981 tgaccattcc gctaaaacgc gcc
//
LOCUS       DAZOYL010000124        10948 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZOYL010000124 DAZOYL010000000
VERSION     DAZOYL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.124"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            117..878
                     /gene="adiY"
                     /locus_tag="ACHUOY_002350"
     CDS             117..878
                     /gene="adiY"
                     /locus_tag="ACHUOY_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR0683112.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1015..2352
                     /gene="adiC"
                     /locus_tag="ACHUOY_002351"
     CDS             1015..2352
                     /gene="adiC"
                     /locus_tag="ACHUOY_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR0683113.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2456..4099
                     /gene="eptA"
                     /locus_tag="ACHUOY_002352"
     CDS             2456..4099
                     /gene="eptA"
                     /locus_tag="ACHUOY_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR0683114.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            4096..4764
                     /gene="basR"
                     /locus_tag="ACHUOY_002353"
     CDS             4096..4764
                     /gene="basR"
                     /locus_tag="ACHUOY_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR0683115.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4765..5865
                     /gene="pmrB"
                     /locus_tag="ACHUOY_002354"
     CDS             4765..5865
                     /gene="pmrB"
                     /locus_tag="ACHUOY_002354"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR0683116.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5841..5930)
                     /gene="pmrR"
                     /locus_tag="ACHUOY_002355"
     CDS             complement(5841..5930)
                     /gene="pmrR"
                     /locus_tag="ACHUOY_002355"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR0683117.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6042..7544)
                     /gene="proP"
                     /locus_tag="ACHUOY_002356"
     CDS             complement(6042..7544)
                     /gene="proP"
                     /locus_tag="ACHUOY_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR0683118.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7808..8674)
                     /locus_tag="ACHUOY_002357"
     CDS             complement(7808..8674)
                     /locus_tag="ACHUOY_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR0683119.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(8671..10899)
                     /gene="crfC"
                     /locus_tag="ACHUOY_002358"
     CDS             complement(8671..10899)
                     /gene="crfC"
                     /locus_tag="ACHUOY_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR0683120.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2741 a         2870 c         2681 g         2656 t
ORIGIN      
        1 gtaaaactcg ttatttttat gcatgtttat attcatcata caattatata accatttccc
       61 ggtatcgctt tgctttagcg agaactggtc ttttattcgc actcaggagt acatgtatga
      121 ggatttgcag cgaccaacct tgtattgttt tattgactga aaaagatgtc tggataaggg
      181 tgaatgggaa agaacctatt agccttaaag ctaaccatat ggcgttatta aattgtgaaa
      241 ataatattat cgacgtctcc tctcttaaca acactttggt tgctcatatt agtcacgaca
      301 tcatcaaaga ttacctccgg tttctgaata aagatctctc gcaaatacca gtatggcaac
      361 gtagcgctac gcccatactc accctgccat gcctgacgcc agacgtcttt cgcgttgccg
      421 cgcaacacag catgatgccc gcagaaactg agtcagaaaa ggaacgaaca cgcgcattat
      481 tattcacggt gctatcccgt tttctcgaca gtaaaaaatt cctttcatta atgatgtata
      541 tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat tgaaagcgat attcacaaag
      601 actggaatct tagtatggta gccagttgtt tatgtcttag cccaagtctg ttaaagaaaa
      661 agctgaaaag cgaaaacacc agttatagcc aaataatcac cacctgccgc atgcgttatg
      721 ccgtaaatga attaatgatg gacggtaaaa atatctctca ggtatcacag tcctgcggct
      781 acaacagtac gtcgtacttt atttctgtct ttaaagactt ctacggtatg acgccgctgc
      841 attatgttag tcagcacaga gaacgaactg tcgcctgatt tttaacctta acgaagagct
      901 atattaataa cggcatcagc gataacccgg tcgataataa ttcaactatc gaatgcaggc
      961 gtatgatatg acgtaattta ttgtcacgaa gctcgccttc gcaggagttt aattatgtct
     1021 tcggatgctg atgctcacaa agtgggctta atccccgtca ccctgatggt gtcggggaat
     1081 attatggggt caggtgtttt tctgttacct gcaaacctgg cctccactgg cgggattgcc
     1141 atttatggat ggttggtgac gattatcggt gcgctggggc tctcgatggt atacgccaaa
     1201 atgtcgttcc tcgatccaag tcctggtggt tcttacgctt acgcccgccg ctgctttggc
     1261 ccgtttctcg gttatcaaac caacgtcctc tactggctgg cctgctggat cggcaatatc
     1321 gctatggtgg tcattggcgt aggatattta agttacttct tcccgattct gaaagatcca
     1381 ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct tcgtcctgct gaacattgtc
     1441 ggtccgaaaa tgatcacccg tgtgcaggcc gttgccaccg tgctggcgct gatccccatc
     1501 gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg aaacctatat ggcggcatgg
     1561 aacgtcagcg gcctgggcac cttcggtgcg attcaaagta cccttaacgt tacgctgtgg
     1621 tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg tggtgaaaaa cccgaaacgc
     1681 aatgtcccta tcgccaccat tggtggggta ttgattgccg ccgtttgcta tgtgctctcc
     1741 accaccgcga ttatggggat gatccctaat gccgcactgc gcgtttctgc ctcgccattc
     1801 ggtgatgccg cacggatggc gttgggtgac accgctgggg ccattgtttc cttctgcgca
     1861 gctgcgggtt gcttaggttc actgggcggc tggacgttgc tggcgggtca aacggcgaaa
     1921 gccgctgccg atgacggact gttcccaccg atttttgccc gtgtaaataa agcgggtacg
     1981 ccagtggcgg ggctgattat cgtcggtatt ttgatgacca tcttccagct cagtagcatt
     2041 tcgccaaacg caaccaaaga gttcggtctg gtatcttccg tgtcggtcat ctttacactg
     2101 gtgccatatc tttacacctg tgcggcgtta ctgctgctcg gccacggtca ctttggtaaa
     2161 gcacgcccgg catatctggc tgttactacc attgccttcc tctactgcat ctgggccgtg
     2221 gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca ccctgatggt catcaccgcc
     2281 atgtatgcgc tgaattacaa ccggctacat aaaaacccgt atcccttaga tgcaccaata
     2341 agcaaagatt aattcccctt aatccagcaa acataaaagc caaccttaag aacttaaggt
     2401 tggcttaatt ttgctttgcg agcatatgcg cactttgttc gatggaaaca ccgtgatgtt
     2461 gaagcgccta ctaaaaagcc cctctttgaa tttactcgcc tggctattgt tggccgcttt
     2521 ttatatctct atctgcctga atattgcctt ttttaaacag gtgttgcagg cgctgccgct
     2581 ggattcgctg cataacgtac tggttttctt gtcgatgccg gtcgtcgctt tcagcgtgat
     2641 taatattgtc ctgacactaa gctctttctt atggcttaat cgaccactgg cctgcctgtt
     2701 tattctggtt ggcgcggctg cacaatattt cataatgact tacggcatcg tcatcgaccg
     2761 ctcgatgatt gccaatatta ttgataccac tccagcagaa agttatgcgc tgatgacacc
     2821 gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct gcgctgattg cctgctggct
     2881 aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt ttccgtggag ccaatattct
     2941 ggtttctgta ctgctgattt tgctggtcgc cgcactgttt tataaagact acgcctcgtt
     3001 gttccgcaat aacaaagagc tggtgaaatc cttaagcccc tctaacagca ttgttgccag
     3061 ctggtcatgg tactcccatc agcgactggc aaatctaccg ctggtgcgaa ttggtgaaga
     3121 cgcccaccgc aacccgttaa tgcagaacga aaaacgtaaa aatttgacca tcctgattgt
     3181 cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc tacccgcgtg aaactaaccc
     3241 gcggctggcg aaagataacg tggtctattt ccctaatacc gcatcttgcg gcacggcaac
     3301 ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt gagcattaca aagaagagct
     3361 ggcacagcac caggaaggcg tgctggatat cattcagcaa gcgggcatca acgtgctgtg
     3421 gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc gtgcctcacc agaacgtcac
     3481 cgcgctgaat ctgcctggtc agtgcatcaa cggcgaatgc tatgacgaag tgctgttcca
     3541 cgggctggaa gagtacatca ataacctgca gggtgatggc gtgattgtct tacacaccat
     3601 cggcagccac ggtccgacct attacaaccg ctatccgccg cagttcagga aatttacccc
     3661 aacctgcgac accaatgaga tccagacctg taccaaagag caactggtga acacttacga
     3721 caacacgctg gtttacgtcg actatattgt tgataaagcg attaatctgc tgaaagaaca
     3781 tcaggataaa tttaccacca gcctggttta tctttctgac cacggtgaat cgttaggtga
     3841 aaatggcatc tatctgcacg gtctgcctta tgccatcgcc ccggatagcc aaaaacaggt
     3901 gccgatgctg ctgtggctgt cggaggatta tcaaaaacgg tatcaggttg accagaactg
     3961 cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac aatttattct ccacgctatt
     4021 gggattaact ggcgttgaga cgaagtatta ccaggctgcg gatgatgttc tgcaaacttg
     4081 caggagagtg agtgaatgaa aattctgatt gttgaagacg atacgctgtt attgcaggga
     4141 ctgattctgg cggcgcaaac cgaaggctac acgtgcgatg gcgtaacaac cgcgcggatg
     4201 gcggaacaaa gccttgaggc cggtcattac agcctggtgg tactggattt agggttaccc
     4261 gacgaagatg gactgcattt tctcgcccgt atccggcaga aaaaatacac cctgccggta
     4321 ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg ccgggctgga tgtcggtgcc
     4381 gatgactatc tggtgaagcc ttttgcgctg gaagagttac acgcccgtat ccgcgccctg
     4441 ctacgacgcc ataataatca gggcgaaagt gagctgattg ttggcaatct gacgctgaat
     4501 atgggtcgcc gtcaggtatg gatgggcggt gaagagttga ttctgacgcc caaagaatat
     4561 gctctgctgt cacggttaat gctcaaagca ggcagtccgg tacatcggga aattctctac
     4621 aacgacatct ataactggga caatgaaccc tcgaccaaca ccctggaagt gcatatccac
     4681 aatctgcgcg acaaagtggg caaagcccgt atccgcaccg tgcgcggctt tggctatatg
     4741 ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt ttctgcgccg accaatatcg
     4801 ctgcgccaac ggctgatatt gaccatcggg gccattttgt tggtgtttga gctgatcagc
     4861 atcttctggc tatggcatga aagtaccgag cagattcagc tgtttgagca ggcgctacgc
     4921 gacaatcgca acaacgatcg ccacatcatg cgtgagatcc gcgaggcggt cgccagcctg
     4981 attgtccccg gcgtctttat ggtcagcctg acgctattta tctgctatca ggcggtacgc
     5041 cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg aagcgcgcac cgccgacaac
     5101 ttaacgccca ttgccattca cagcgccacc ctcgaaatcg aagcggtggt ttcggcgttg
     5161 aacgatctgg tcagtcgcct gacgagcacg ctggataacg aaaggttatt taccgctgac
     5221 gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt tgcatctgga actgctggcg
     5281 aaaacgcatc acattgatgt agcaccgtta gtggcacggc ttgatcagat gatggagagc
     5341 gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat tttcttccgg taattatcaa
     5401 catgtaaaac tgctggaaga tgtcattctc ccctcgtatg acgaactcag taccatgctc
     5461 gaccagcgac agcaaaccct gctattgcca gagagcgccg ccgacatcac cgttcagggt
     5521 gatgcaaccc tgctgcggat gttattgcga aacctggtag aaaacgccca tcgttacagc
     5581 ccacaaggca gcaacattat gattaagctg caagaagatg gcggagcggt catggccgtt
     5641 gaagatgaag gaccgggtat tgatgagagt aaatgcgggg agttgagtaa agcgtttgta
     5701 cgtatggaca gccgttatgg cgggattggt ctggggttaa gtattgtcag ccgcattaca
     5761 cagttgcatc acgggcagtt tttcctgcaa aaccggcaag agacttccgg cacgcgggcc
     5821 tgggtacggc tgaagaaaga tcagtacgta gcaaaccaga tataaagaaa gctgctgacc
     5881 accagcacac tgaacacggt agttaaactt tcataaacac ggtttttcat tgcactctcc
     5941 tccgttaacc tggaggagag tatgcgcgtc agagattaag tgaaccttaa gagttcaatt
     6001 ggcctgataa gacagcgtca catcaggcca tccgtttcag cttattcatc aattcgcgga
     6061 tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg caatctcgtg gtcaatatca
     6121 tcgattttct gctcgatatt atcgtaatgc tcgacgagaa tttccttcgc ttcctgtata
     6181 tctgacgccg ccggtgtcgc acctttcaac ggacgatttg ccgtctcttt catggttacg
     6241 ccggtgatta aaccaatcac cgccactacc atcaggtaat aggcaggcat catcagattc
     6301 tgcgagcttt cgaccagcca ggccgccagt gttggcgtca gaccggcaac cagcaccgaa
     6361 atattaaatg ccgccgccag cgcgctgtaa cggatatgcg tcgggaacat cgctggcaag
     6421 gtagaagcca taacgcccgt aaagcagtta aggatcaccg ccagcatcag taacccggca
     6481 aaaatcaggc cgatgacgtt actgttaatc agaataaacg ccgggatcgc caacacaaac
     6541 agggcaacac taccaagtag cacaaacgga cgacggccaa aacggtcact cagcaagccc
     6601 atcaccggct ggacaaacag cataccgatc ataatggcga taataatcag caccccgtgg
     6661 tcttcggagt aatgcaggtt atgcgacaaa taactcggca tataggtcag caacatgtag
     6721 taagtcacgt tggtggcaat taccagacca atacatgtca acaggctgcg ccagtattta
     6781 gtggcaatct ctttaaacga gactttcggg ccatcctgca aaccttcgcg gtcgccctgt
     6841 tccagtttat caacatgctg ctggaacgcc ggagtttctt ccagcgcatg gcgcaggtaa
     6901 agcccgataa tccctaacgg cagagcaata aagaacggaa tacgccagcc ccagtcgagg
     6961 aagttcgctt cgccgacaat ggtcgaaatt aacaccacca cgcccgcacc cagcacaaac
     7021 ccggcaatag aaccgaagtc cagccagctg cccataaagc cacgtttacg gtcaggggag
     7081 tattccgcaa caaatatcga cgccccggta tattcaccgc cgaccgagaa accttgtgcc
     7141 atcttacaga tcaacagcag aatcggtgcc caaataccaa tcgtgtcgta ggacggtatt
     7201 aagccaatac agaacgtact gatcgacata atcacaatag tgatagcgag gatcttctgg
     7261 cgaccatatt tatcgcccaa cataccaaag aagagcccgc caagcggtcg aatcagaaag
     7321 ggaacggaga aagtggcaag tgcagcaacc atctgcacgc tggggtcagc ccccgggaaa
     7381 aaaactttac ctaatgcgta agcaacaaaa ccataaacac caaaatcgaa ccattccatt
     7441 gcattaccca gtgatgctgc ggtaatggct ttacgcagtt taccgtcatc aataatggtg
     7501 acatcacgaa gggtaatcgg ttttactttt ttccttttca gcatagcttt cctcgcagag
     7561 ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg gccgcaaaag gggagcggga
     7621 attgcaggat gcaatctctg taacaagcgt agcgggtttg cttacaccct ccggttaagg
     7681 aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt aatgaccttt ttaccctacc
     7741 agtgtttaaa tttgtgatca acttcacaca tggatttcgt ttaggactca ttgatgtaac
     7801 tgattattta cgccaaattt acagagaaga tttcctcgac cattgccccc accaaacgct
     7861 gcgctgaaca gagtcgtggc atccccagcg cgactgtttg ccagcgttgg gtcactgacc
     7921 agcaaacggg ctgtttatct gccgtggacc actttcccag ccaacttaaa aaatcagcat
     7981 gctcccctcc cgaagaggca aaccactgat ggtaatagtg acgcgtggtc ggtgccgcat
     8041 tgatactctc tgccagatgt tgtgtcgctt tctcgcgcaa atcacccagt aacaacacca
     8101 ccgcttgcgg gcttcccaga cggcaggcat cgccaaatgc accccagcgc aactcctcca
     8161 gaacgataaa acagcgccca ggcaacgacc aggcgttata cgccccagca cgccagcggg
     8221 taaaaatttg ctcgctctga tcacctgcct gcaccagtag gcctcgttgc gttaacgcct
     8281 tttccttcca gcgacaacgg gctgaaaacc actcatacaa ggcggcaatt tgctggggga
     8341 gctccgggaa agcagagtgc tggcggtgat ccattttttg cagcagcgcc agatgctgcg
     8401 cgatctgcgt cagcgccaga tgaccgggat ccagcatcga cgccagtttt tccaccattg
     8461 tcagtcgcag gctaagcggc gcatttaaca tgccgccgtt cgtccatgtc tgaagctgca
     8521 cacgcgaaag aagttcctgc atcagtcgtt cacgaaactg ctgctgatgg gcttgtggaa
     8581 ggcgggcatc atcgccctgc gccagatcca ctaaaaagcg gggataaacc gactccagca
     8641 cgcgaccggg gccgtccagt aacgtcttgg tcaatatcgt tctgccgtga aaagtgtttg
     8701 aatatcatcg cgtaacagct gggcgtcggt gtaaatccag ccgtgagtca tcacagtctg
     8761 ctgcaattgc tgctgcatca gcctgaccac cgattcattt tgttgacgca gagccaggct
     8821 ttcgcgtaaa cgcgtctgta attccgtcaa acatgaagcg aactcagcgc aaaaagtatt
     8881 catgcctgcc gtaacagatt catcgacctg ctctgccaga actttagcca tttgttggca
     8941 ataaagatcg acttctgcgc ttaaagctcg ttgcaacaca ctgtaatcaa ccgtctctgt
     9001 cggggatttc tcatttcccc gtccccagtc gggctgattc aaccagcgcg aaaaagtctc
     9061 acgcacaacg cctaaacgcg tgctctgctc gtccgttgca tcctggcgcg aaatgacttc
     9121 actgaacagc tggcgagtgt tgacgtgggg aactactccg tgaaaaacag gaaaatgaac
     9181 cccaggacga aaccctgact cgctcaattc cattttgact tgttgctcaa tgggccgaat
     9241 aacatcggtt aacacccggc aaagggtgga ttcaagctcg gcaaaacgca gcgtaaagtc
     9301 gcgactgatg gtgttctgcg ccgtctgtaa cagtgtctca cagcaggtac gaatctcgct
     9361 taacggctcc gaatcatcct gaaacaaggc ggctaactgc gcattcagcg catcttgctg
     9421 ttgacgcaga aagtggttgg cggaggtcag ggccagctcg atttcatgtt taatctcgcc
     9481 gctcacctgc gcctgattga gttgcaatag ctgcaaactt tcttcgacct gatggatatt
     9541 ttgccgcaat tgttcacaag cgacgtttaa cccgtgcgca cgaaaatcca ggtattcccg
     9601 cgcctgctgc gcgtaattca acagtttatg cgcagcagat cgcagagcat acaacgaggc
     9661 gttagcgtaa gcggcatgaa gcaacgcctg aattggctgg gcgaacagcg aatcttccca
     9721 caactgatcg gcagcatgac gaatatgttc gaggtccgcc agatcggcat gacgccagcg
     9781 cctgccgagc gcggcatggg caaaatcttc cacccagcgt tgttgctctg gcgctggtaa
     9841 cttaccgttg ttggctaact catggcgcgc tcgatttgcc aagtagcccc acatcgacga
     9901 caccggaaat atctgctgtg gcgtaataca gcctttcatc agcgtcccgg aaatcagtgc
     9961 ccgcacctgg tcggcgtcgt cactgttacg atcctgttga tcgaacttat tgaccagcac
    10021 atacagtggc accgattgcc ccaccgccaa aatcgcctca cggacctctt catcggagat
    10081 cgatttcagt tgcgtataat ccagcaccgc cagtaccgcc gaggcgcgtg ccagctgctg
    10141 gttaagcatt ttttgcagat gcggttgccc ggcttcattt ggcccggggg tatccagtaa
    10201 cgtcaactga ccgggataac tctccagccc cgccagatgg acaaactcca cttcaatcac
    10261 gggaatatgc tcaatggcgg cgtaagcaga aaaaggaaaa tcgacgtcca gcgccttcgc
    10321 cagtcgcact aaatcattca aacttttcag acaatgaaaa ataggctggg cacccagata
    10381 atatttttcg aaagcgacgc cattttcgat ccgctgcata agcgcacgca tatctttatc
    10441 tatttccagc acatcggtca gatgcttaat atcgcaatca cgcaggcgct gttgtaattg
    10501 ttgaattaaa caatcgattg gcgcgacatg tgaaaaatgc agtaccggtt ccttttgccc
    10561 gggcgtatgg cgaataagcg tcggcagcgc agtcattggg cgattacgat taggcagaac
    10621 ctccgtacca acaatggcat taatggtggt tgatttccct gctttcatag taccgacaat
    10681 cgccagcacc atttccagtc gggaaatttt actcaactca ttattcagca tcgcgtgacg
    10741 ttcggcgata ttaggctgac tccagggtaa agccagttgt ggcgcgtcgt ctccgggtac
    10801 agagagaggc attttttcca gtaactgcaa ctgttggcga gaaagctgta acaggcgttc
    10861 agcctcctga cttaactcat acagggtctg tgtgtacata gaaaattctt ccttaaagca
    10921 aattttgtta cttttattta gccagatt
//
LOCUS       DAZOYL010000125        10912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZOYL010000125 DAZOYL010000000
VERSION     DAZOYL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.125"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            139..480
                     /locus_tag="ACHUOY_002359"
     CDS             139..480
                     /locus_tag="ACHUOY_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR0683121.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(471..1397)
                     /locus_tag="ACHUOY_002360"
     CDS             complement(471..1397)
                     /locus_tag="ACHUOY_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0683122.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHCRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            1487..2485
                     /locus_tag="ACHUOY_002361"
     CDS             1487..2485
                     /locus_tag="ACHUOY_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR0683123.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2482..2700)
                     /locus_tag="ACHUOY_002362"
     CDS             complement(2482..2700)
                     /locus_tag="ACHUOY_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR0683124.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2702..4717)
                     /gene="ligA"
                     /locus_tag="ACHUOY_002363"
     CDS             complement(2702..4717)
                     /gene="ligA"
                     /locus_tag="ACHUOY_002363"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR0683125.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4788..5774)
                     /gene="zipA"
                     /locus_tag="ACHUOY_002364"
     CDS             complement(4788..5774)
                     /gene="zipA"
                     /locus_tag="ACHUOY_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR0683126.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6004..6765
                     /gene="cysZ"
                     /locus_tag="ACHUOY_002365"
     CDS             6004..6765
                     /gene="cysZ"
                     /locus_tag="ACHUOY_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR0683127.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6950..7921
                     /gene="cysK"
                     /locus_tag="ACHUOY_002366"
     CDS             6950..7921
                     /gene="cysK"
                     /locus_tag="ACHUOY_002366"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR0683128.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8305..8562
                     /gene="ptsH"
                     /locus_tag="ACHUOY_002367"
     CDS             8305..8562
                     /gene="ptsH"
                     /locus_tag="ACHUOY_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR0683129.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8607..10334
                     /gene="ptsI"
                     /locus_tag="ACHUOY_002368"
     CDS             8607..10334
                     /gene="ptsI"
                     /locus_tag="ACHUOY_002368"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR0683130.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10375..10884
                     /gene="crr"
                     /locus_tag="ACHUOY_002369"
     CDS             10375..10884
                     /gene="crr"
                     /locus_tag="ACHUOY_002369"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR0683131.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2657 a         2816 c         2702 g         2737 t
ORIGIN      
        1 catcctgcac gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt gctatctgcg
       61 atttgtaaga ttgatttttc ttctcaagtt ccttgttctt ctgccgatat tacctcgtac
      121 agtgacgagg actgaacaat ggccactatc tcttcaactt caattccttc catacaaaca
      181 cagagtagta acagggcatc acaaggtagc gatgttgctt caaaaatcgc gcggataagt
      241 cagcaaatca tcaaactcac tcaacaaatt aaagagattg tagacactag cggcagtgct
      301 gaagacaaac aaaaacaggc cgaattgatt caacagcaaa tcaccctact ggaaacacaa
      361 ctggcgcagt tgcagagaca gcaggcagaa aaggcgcagg agaaagagca gcgtttgtct
      421 ctgaatgtat cccttctaaa tcctgttgaa aatacgactc atatcgatat ctatatctga
      481 tacaacggag ccccttcccg accagccatt agcgcattgc cctggtctcg taccacgtcc
      541 cataacgctt cagcggcggt ggaaagtgaa cggttcttcc tgcgcaccag catcaactgg
      601 cgttcaacaa ccggcgtaat gcgctttacc accagtgggc taccttcagg caatggtagc
      661 gccagtgcgg gaagaatact gatgccgatg cctgcggcta ccatcgggaa cagcgtcgcc
      721 ggatgtccaa tctcctgcac aatattcgcc tgaataccat tgcgcgccag cgctgcgtca
      781 atcagcggtc ggctgcctga cgcgtaatcc tgcaacacca atttcgctcc ctgtaatgcc
      841 tgccagggta cataatcttc cacggccaaa gcgctatcgc gatggcaaag cagaaagaaa
      901 ggctcggaaa gaatcgcttc acattgcaga tcgcccactg ggcctggatc gatgacgatg
      961 caaaaatcga catctccctg gcgaatactt tccatcaccc actgctgcgg acgatcgtgc
     1021 aggacaaact gaatatctgg atagcggcaa tggctttcag caatgcattg cggaataaga
     1081 tgcgcggaaa tggtctggct ggcagcgacc cgaacttttc cgctcagttg ttgccccata
     1141 cgcccggtat cgcgcaacgt gctgttaagt tcatccagca gtcgctcaag acgcaaagcc
     1201 aactgctgcc ctgcatctgt aagcaccact tcacgcgtgg ttctgtccag caggcgaaca
     1261 ccagtatgat tttccagctc cttcacactg tgacttactg ccgactggct caagccgata
     1321 cgctctcctg cacgactaaa acttttctcc tgcgctactg tgacgaaaac ctttaattgt
     1381 tttaaagaat aattcatctg ttttcttcat gaatggatgc aataaatcaa ttttatttct
     1441 caaactggaa gaagcacaat agaaccatcg atcatctgga gtctttatga aactttttcg
     1501 tatcctcgat cctttcacct taaccctgat cacggtggtg ttgctggcct ctttctttcc
     1561 ggccagaggc gatttcgtcc ccttctttga aaatctgacc accgcagcta ttgccctgct
     1621 gttctttatg cacggcgcga agttgtcgcg tgaggcgatt attgctggcg gtggtcactg
     1681 gcgactgcat ttgtgggtaa tgtgcagcac cttcgtgctg tttccgattc tgggtgtact
     1741 gtttgcctgg tggaaaccgg taaatgtcga cccgatgctc tactccggtt ttctctactt
     1801 gtgcattctc ccggctaccg tgcagtctgc aatcgccttc acgtcaatgg cgggcggtaa
     1861 cgtcgcggcg gcggtttgtt ctgcgtcagc atccagcctg ctggggattt tcctttcacc
     1921 attgctagtt ggtctggtga tgaatgttca cggtgcaggg ggcagccttg agcaggtcgg
     1981 taaaattatg ctgcaactgc tgctgccgtt tgtgttgggg catctttccc ggccgtggat
     2041 tggtgactgg gtgtcgcgca ataaaaaatg gattgcgaaa actgaccaga cgtccattct
     2101 gttggtggtt tatacagcgt tcagcgaagc cgtcgttaat ggtatctggc ataaagttgg
     2161 ctggggatca ttgctgttta tcgtggtggt cagctgcgtt cttctggcta tcgtgattgt
     2221 agttaacgtc tttatggcac gccgactggg cttcaataag gcagatgaaa ttactatcgt
     2281 cttttgtggt tcgaaaaaga gtctggcaaa tggcatcccg atggcaaaca ttctgttccc
     2341 cacatcggtg atcggtatga tggtgctgcc cctgatgatt ttccatcaga tccaattgat
     2401 ggtctgtgcg gtgctggcgc gtcgatacaa acgccagacc gaacagttac aggcgcagca
     2461 ggaaagcagc gccgataaag cttaaagcgg acgcttcagg ggctggacca gttgcgtcag
     2521 cccctcggtt ttaatcagca gcgtgatttg cattagctca ccgagcttcc ctgccgggaa
     2581 ctcatctttg cgggcaaacc acagcagata ctcctccggc aggtcgatta agcgacgccc
     2641 tttgtatttg ccaaacggca ttatcgtatt ggctatttca atcagctgct ctttttccat
     2701 ctcagctacc cagcaaacgc agcatttccg cttcgtcgat gacttcaatg cccagttcct
     2761 gcgccttcgc cagtttagat cctgcagctt cacccgctat caccagatcg gttttcttcg
     2821 acacgctgcc cgcgactttc gcgcccagtt cgaccagtcg agctttagcg tcatcacgcg
     2881 acatctggct taagctgccc gtaagcacca cggttttacc agcaaacggg ctgtcaatct
     2941 cttccgcgtt gataacgatc ggcgcaggcc agtgaacacc ttccgccaac agctcgctga
     3001 tgacattgcg gttgctttct tcggcaaaga agttgtgaac gtgggatgca acgacaatgc
     3061 caacatcagg caccttttgc agctcttcaa tcgaagcggc ttccagcact tccagcgtgc
     3121 cgaaatatgc cgccagacct gctgcggtgg cctcgccgac ttcacggatg ccaagtgcat
     3181 agaggaagcg agcaaaggtg gtttctttcg ctttttccag cgcgttaacc acgttttgtg
     3241 ccgattttgg ccccatacgc tccagtccgg tcagtttgcc tgcggtgagt ttgaacagat
     3301 ctgccggagt gtggacatat tctttttcaa ccagctgatc gatgattttg tcgcccattc
     3361 cgtcaacatc catcgcacgg cgggaaacaa agtgtttcag cgactcttta cgctgcgcac
     3421 cgcaaatcag gccaccggta cagcgggcaa ccgcttcacc ttccacacgc tcaacgtcag
     3481 aaccacatac cggacaatgc gtcgggaata caacctcacg ggtatcttcc gggcgttcag
     3541 aaagcacgac gttaaccacc tgcgggatca cgtcgccagc gcggcgaatc accactttat
     3601 cgccaatgcg taaaccaaga cgttcgattt catccgcatt gtgtaaggtt gcgttactca
     3661 ccagcacgcc agcaacatgg acaggttcca gacgcgcaac aggcgtaatc gcgccagtac
     3721 gcccaacctg aaactcgacg tcacgcacaa aggtcatctg ctcctgcgcc gggaatttaa
     3781 acgctaccgc ccagcgcggg gcacgcgcga caaagccaag ctgctcctgc tgttccagtg
     3841 agttgacctt aatcaccacg ccgtcgatat caaagcccag cgtcgggcgg tcttcttcca
     3901 ctttgtgata gaacgccagc acttcttccg ccgattcaca aagcgttacc cgatcgctga
     3961 ccggcaaccc ccacttttta aattgcagta aacggccaag atgagtatcc ggcagctcgc
     4021 caccttccag aacaccaacg ccatagcaga aaaaagtgag cggtcgcttc gctgtaatac
     4081 gcggatcaag ctgacgcagt gaaccagctg ccgcattacg tgggttagca aaaactttcc
     4141 cgcccgtgcg tcgcgcatct tcgttaatct tttcgaaccc cgcctgcggc aggaacactt
     4201 caccacgcac ttccagacgc gccgggatat tctctccgtg cagcttcagc ggaatggcgc
     4261 gaatagtacg cacattagac gtgatatctt ccccggtggt gccatcgcca cgggtcgcgg
     4321 cactgactaa aacgccattt tcatacagaa tactgacggc aaggccatcc agcttcagct
     4381 cacagcacca ggtgactttc tcgttgtttt tcagacggtc ctgcacacgt ttgttgaaag
     4441 caagaaagct ttcttcatca aaaacgttat ccagtgacag cattggtact tcatggcgta
     4501 tctggctgaa agccgccagc ggcgcagcac ctacacgttg ggtaggcgaa tcaggcgtaa
     4561 tcagttctgg atgtttggtt tccagctcgc gcagttcgcg catcagcctg tcgtattcag
     4621 cgtcgggaat ttccggcgca tccatcacat gataaagata ttcatgatgg cgaagcgtcg
     4681 ttcgcagttc tgtcagttgt tgttcgattg attccatatc gcaccatcaa tgctaaaaac
     4741 ccccgacaag cgggggttcg aagaggagtt aatttgcctt aagtgtatca ggcgttggcg
     4801 tctttgactt cgcggatgat gtcctggtac tcgcgcaatt tctgcggagt catcatacgg
     4861 cgctgatcgt caagcacgac accgcccact tcatcggcaa tatgctgcgc agattgcagc
     4921 atcagcttga agttctgcag ctcgtcaccg taagacggta cctgcataaa gatagtgaca
     4981 cccggagtag tgaaatcctt catttcagga tcaaaggttc ccggtttcac catattcgcc
     5041 aggctgaata acgccgggcc gctgccatcc gggctaagat gacgatggta aatattcata
     5101 tcgccaaaaa tgaagcccgc ttgttgaatg ctgttaagaa gcagttcacc gtttagctcg
     5161 ctaccgtgat gcgccgcgac gttcatgata atcaccgctt ctttgcgctt cggtttatcc
     5221 ataactggag ccggttccgc tacaggctca ggctgtggtg ccgctacggg ttctgcaggc
     5281 tggaaagcct gttgtgccgg ttgcggtgct gaatgcacag gctgcggcgc tggcgcgacc
     5341 tgtggcgaaa ctggctgctg caacggctgt tcaggctgcg gctgataggc aggctgctgc
     5401 atcggctgcg ctggacgcgg agcatgttgc ggcggtacct gcgcttcagg cggctgctgg
     5461 atcggttgac gcggctgcgc agacgcataa ggcggttggt actggtgttg cggcgacgga
     5521 cgagcagcct catgctcctg agcgttagcc ggggcatgat tcacgcggtg aacacgaacc
     5581 tcaccaacgc cctcatcatc ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac
     5641 ttcattcgtt ttaatggccg atcgcggaac atagaagatc gttctttacg gctggtccag
     5701 aaaccatgta ccagtaaagc gattatggcg atcgcgccaa caatgattaa tatcagacgc
     5761 aaatcctgca tcattatatt ctctgttgtt ctaacacctt gccaccacgg caaacattta
     5821 ctcactaaga gtatttgccg attacctcaa gtgcaagtgc actattaact ttcacagcac
     5881 aaagatagat gaaatcgtgc tttttgctgt tttttcgaac atatcctaac tgtccattgc
     5941 gcaattaccc ggtaaaatac gcagaatttt cctgggattg gtcaaaagga gctcatcctg
     6001 attatggttt catcattcac atctgcccca cgcagcggtt tttactattt tgcgcaaggc
     6061 tggaagctcg tctcgcaacc tgggattcgg cgtttcgtta ttttaccgct gctggtcaat
     6121 attttgttga tggggggcgc attctggtgg ctctttacac agctcgatgt ctggatcccg
     6181 actctcatga gttacgttcc ggactggcta caatggctga gttatctgtt gtggcctctg
     6241 gctgtcatct ctgtgctgtt agtgtttggc tatttcttct ccacgattgc taactggatt
     6301 gccgctccgt ttaacggttt attggctgaa caactggaag cacgattgac tggcgctaca
     6361 ccgccagata ccgggatttt cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa
     6421 tggcaaaaat ttgcctggta tctgccgcgc gcaattgtat tactaattct ttacttcatc
     6481 cctggtattg ggcaaaccgt cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta
     6541 gccatccagt attgcgatta ccccttcgat aaccacaaag tgccgtttaa agagatgcgc
     6601 accgccctgc gcacacgcaa aatcaccaat atgcagtttg gtgctttaac cagcctgttt
     6661 acgatgatcc cgctgcttaa tctgttcatc atgcccgttg ccgtttgtgg cgcgacggcg
     6721 atgtgggtcg attgctatcg cgataaacac gcgatgtggc ggtaacaatc taccggttat
     6781 tttgtaaacc gtttgtgtga aacaggggtg gcttatgccg ccccttattc catcttgcat
     6841 gtcattattt cccttctgta tatagatatg ctaaatcctt acttccgcat attctctgag
     6901 cgggtatgct acctgttgta tcccaatttc atacagttaa ggacaggcca tgagtaagat
     6961 ttttgaagat aactcgctga ctatcggtca cacgccgctg gttcgcctga atcgcatcgg
     7021 taacggacgc attctggcga aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg
     7081 tatcggtgcc aacatgattt gggatgccga aaagcgcggc gtgctgaaac caggcgttga
     7141 actggttgaa ccgaccagcg gtaataccgg gattgcactg gcctatgtag ctgccgctcg
     7201 cggttacaaa ctcaccctga ccatgccaga aaccatgagt attgaacgcc gcaagctgct
     7261 gaaagcgtta ggtgcaaacc tggtgctgac ggaaggtgct aaaggcatga aaggcgcaat
     7321 ccaaaaagca gaagaaattg tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt
     7381 cagcaatccg gcaaaccctg aaattcacga aaaaaccacc ggtccggaga tctgggaaga
     7441 taccgacggt caggttgatg tatttattgc tggcgttggg actggcggta cgctgactgg
     7501 cgtcagccgc tacattaaag gcaccaaagg caagaccgat cttatctctg tcgccgttga
     7561 gccaaccgat tctccagtta tcgcccaggc gctggcaggt gaagagatta aacctggccc
     7621 gcataaaatt cagggtattg gcgctggttt tatcccggct aacctcgatc tcaagctggt
     7681 cgataaagtc attggcatca ccaatgaaga agccatttct accgcccgtc gtctaatgga
     7741 agaagaaggt attcttgcag gtatctcttc tggagcagct gttgccgcgg cgttgaaact
     7801 acaagaagat gaaagcttta ccaataagaa tattgtggtt attctaccat catcgggtga
     7861 gcgttattta agcaccgcat tgtttgccga tctcttcact gagaaagaat tgcaacagta
     7921 atgccagctt gttaaaaatg cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc
     7981 ttcaaacttt cgcccctcct ggcattgatt cagcctgtcg gaactggtat ttaaccagac
     8041 taattatttt gatgcgcgaa attaatcgtt acaggaaaag ccaaagctga atcgatttta
     8101 tgatttggtt caattcttcc tttaacggca taatgtttaa tgacgtacga aacgtcagcg
     8161 gtcaacaccc gccagcaatg gactgtattg cgctcttcgt gcgtcgcgtc tgttaaaaac
     8221 tggcgctaac aatacaggct aaagtcgaac cgccaggcta gactttagtt ccacaacact
     8281 aaacctataa gttggggaaa tacaatgttc cagcaagaag ttaccattac cgctccgaac
     8341 ggtctgcaca cccgccctgc tgcccagttt gtaaaagaag ctaagggctt cacttctgaa
     8401 attactgtga cttccaacgg caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact
     8461 ctgggcctga ctcaaggtac cgttgtgact atctccgcag aaggcgaaga cgagcagaaa
     8521 gcggttgaac atctggttaa actgatggcg gaactcgagt aatttcccgg gttcttttaa
     8581 aaatcagtca caagtaaggt agggttatga tttcaggcat tttagcatcc ccgggtatcg
     8641 ctttcggtaa agctctgctt ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt
     8701 ctgccgacca ggttgatcag gaagttgaac gttttctgag cggtcgtgcc aaggcatcag
     8761 cccagctgga aacgatcaaa acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca
     8821 tctttgaagg gcatattatg ctgctcgaag atgaggagct ggagcaggaa atcatagccc
     8881 tgattaaaga taaacacatg acagctgacg cagctgctca tgaagttatc gaaggtcagg
     8941 cttctgccct ggaagagctg gatgatgaat acctgaaaga acgtgcggct gacgtacgtg
     9001 atatcggtaa gcgcctgctg cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca
     9061 ttcaggatga agtcattctg gttgccgctg acctgacgcc gtccgaaacc gcacagctga
     9121 acctgaagaa ggtgctgggt ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta
     9181 tcatggcgcg ttctctggaa ctgcctgcta tcgtgggtac cggtagcgtc acctctcagg
     9241 tgaaaaatga cgactatctg attctggatg ccgtaaataa tcaggtttac gtcaatccaa
     9301 ccaacgaagt tattgataaa atgcgcgctg ttcaggagca agtggcttct gaaaaagcag
     9361 agcttgctaa actgaaagat ctgccagcta ttacgctgga cggtcaccag gtagaagtat
     9421 gcgctaacat tggtacggtt cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg
     9481 ttggtctgta tcgtactgag ttcctgttca tggaccgcga cgcgctgccc actgaagaag
     9541 aacagtttgc tgcttacaaa gcagtggctg aagcgtgtgg ctcgcaggcg gttatcgttc
     9601 gtaccatgga catcggcggc gacaaagagc tgccatacat gaacttcccg aaagaagaga
     9661 acccgttcct cggctggcgc gctatccgta tcgcgatgga tcgtaaagag atcctgcgcg
     9721 atcagctccg cgctatcctg cgtgcctcgg ctttcggtaa attgcgcatt atgttcccga
     9781 tgatcatctc tgttgaagaa gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg
     9841 aactgcgcga cgaaggtaaa gcgtttgacg agtcaattga aatcggcgta atggtggaaa
     9901 caccggctgc cgcaacaatt gcacgtcatt tagccaaaga agttgatttc tttagtatcg
     9961 gcaccaatga tttaacgcag tacactctgg cagttgaccg tggtaatgat atgatttcac
    10021 acctttacca gccaatgtca ccgtccgtgc tgaacttgat caagcaagtt attgatgctt
    10081 ctcatgctga aggcaaatgg actggcatgt gtggtgagct tgctggcgat gaacgtgcta
    10141 cacttctgtt gctggggatg ggtctggacg aattctctat gagcgccatt tctatcccgc
    10201 gcattaagaa gattatccgt aacacgaact tcgaagatgc gaaggtgtta gcagagcagg
    10261 ctcttgctca accgacaacg gacgagttaa tgacgctggt taacaagttc attgaagaaa
    10321 aaacaatctg ctaatccacg agatgcggcc caatttactg cttaggagaa gatcatgggt
    10381 ttgttcgata aactgaaatc tctggtttcc gacgacaaga aggataccgg aactattgag
    10441 atcattgctc cgctctctgg cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt
    10501 gcggaaaaaa tcgttggtga tggtattgct atcaaaccaa cgggtaacaa aatggtcgcg
    10561 cctgtagacg gtaccattgg taaaatcttt gaaaccaatc acgcgttctc tatcgaatct
    10621 gatagcggcg ttgaactgtt cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa
    10681 ggcttcaagc gtattgctga agaaggtcag cgcgtgaaag ttggcgatac tgtcattgaa
    10741 tttgatctgc cgctgctgga agagaaagcc aagtctaccc tgactccggt tgttatctcc
    10801 aacatggacg aaatcaaaga actgatcaaa ctgtccggta gcgtaaccgt gggtgaaacc
    10861 ccggttatcc gcatcaagaa gtaattcttg ccgcagtgaa aaatggcgcc ca
//
LOCUS       DAZOYL010000126        10737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZOYL010000126 DAZOYL010000000
VERSION     DAZOYL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.126"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            93..1133
                     /locus_tag="ACHUOY_002370"
     CDS             93..1133
                     /locus_tag="ACHUOY_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR0683132.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="ACHUOY_002371"
     CDS             complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="ACHUOY_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR0683133.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2351..4510)
                     /gene="aas"
                     /locus_tag="ACHUOY_002372"
     CDS             complement(2351..4510)
                     /gene="aas"
                     /locus_tag="ACHUOY_002372"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR0683134.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5096..6127
                     /gene="galR"
                     /locus_tag="ACHUOY_002373"
     CDS             5096..6127
                     /gene="galR"
                     /locus_tag="ACHUOY_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR0683135.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="ACHUOY_002374"
     CDS             complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="ACHUOY_002374"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR0683136.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7518..8453
                     /gene="lysR"
                     /locus_tag="ACHUOY_002375"
     CDS             7518..8453
                     /gene="lysR"
                     /locus_tag="ACHUOY_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR0683137.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8440..9087)
                     /locus_tag="ACHUOY_002376"
                     /pseudo
     CDS             complement(8440..9087)
                     /locus_tag="ACHUOY_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9218..10636)
                     /gene="araE"
                     /locus_tag="ACHUOY_002377"
     CDS             complement(9218..10636)
                     /gene="araE"
                     /locus_tag="ACHUOY_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR0683138.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         2737 a         2989 c         2612 g         2399 t
ORIGIN      
        1 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
       61 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
      121 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
      181 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
      241 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
      301 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
      361 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
      421 tccgcgaagc gctgcatgac agcctcaagc gcctacacac tgattacctc gatctttatc
      481 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
      541 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
      601 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
      661 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
      721 tgttaaaccg cagttttgaa gtaggtctgg cagaagtaag ccagtatgaa ggggtcgaac
      781 tgctggccta ttcgtgccta ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
      841 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
      901 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggccta gaccctgctc
      961 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
     1021 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
     1081 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
     1141 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
     1201 tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
     1261 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
     1321 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
     1381 gagcggaacg acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg
     1441 cagcagctcg tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg
     1501 catacagcgt gacatggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac
     1561 gataccaatc gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag
     1621 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
     1681 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
     1741 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
     1801 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
     1861 ggcgacgagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
     1921 cgctatggta gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa
     1981 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
     2041 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
     2101 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
     2161 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
     2221 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
     2281 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
     2341 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
     2401 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
     2461 cagcaagctc cggtacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
     2521 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
     2581 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
     2641 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
     2701 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
     2761 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
     2821 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
     2881 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
     2941 cgttgataga aacgacaggc gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc
     3001 caaatttatc ctgccaaagc tgtttggtac tttcttgtaa tttttctgcg cctgccacca
     3061 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
     3121 tcgaggtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
     3181 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
     3241 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
     3301 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
     3361 cgacgccttt cggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
     3421 gctgctgttt tacctgtgcc agacgtggca tcagcagatg cgcgaagatc catactttgt
     3481 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
     3541 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
     3601 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
     3661 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
     3721 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
     3781 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
     3841 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
     3901 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
     3961 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
     4021 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
     4081 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
     4141 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
     4201 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
     4261 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
     4321 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
     4381 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
     4441 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
     4501 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
     4561 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
     4621 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
     4681 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
     4741 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
     4801 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
     4861 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
     4921 agtcaatcgc aacagcattt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
     4981 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccgcccc ttgaaccaac
     5041 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
     5101 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
     5161 taataattca cccaaagcca gcgaagcgtc ccggctggct gtgcatagtg caatggagtc
     5221 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
     5281 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
     5341 acaggtggct tatcacaccg gtaatttttt attgattggc aatggttacc acaacgaaca
     5401 aaaagagcgt caggccattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
     5461 tgccaaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
     5521 gctgatcaac cgtatcctgc ctggcttcga aaaccgttgt attgctctgg acgatcgtta
     5581 cggtgcctgg ctggctacgc gtcatttaat tcaacaaggt catacccgca ttggttatct
     5641 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
     5701 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggtgaac cagacgaaag
     5761 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
     5821 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
     5881 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
     5941 tccgcgcctg accaccgtgc gttacccaat cgtgacaatg gcgacccagg ctgccgaact
     6001 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
     6061 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
     6121 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
     6181 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt
     6241 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg
     6301 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
     6361 cgcccgattc acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
     6421 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
     6481 tcagatcgtt gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
     6541 tccgcacctg agtaattaac acgccagact gcgctaccag aaagcgaccc ggttcaattt
     6601 ccagtttcac agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac
     6661 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
     6721 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgt accatagcac
     6781 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
     6841 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
     6901 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
     6961 ccgggttaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca
     7021 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
     7081 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
     7141 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
     7201 gcattaatcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
     7261 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
     7321 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
     7381 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
     7441 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat agattaccgg
     7501 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
     7561 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
     7621 tcagccgcga actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac
     7681 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
     7741 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
     7801 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
     7861 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
     7921 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
     7981 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
     8041 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
     8101 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
     8161 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
     8221 gggcgggggt aggtgtttcg gtggttaacc cactcaccgc actggattat gcggcaagcg
     8281 gtttagtggt acggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
     8341 tgcaccgccc gtcatcagcg ctggttcagg cgtttagtga gcatttacaa gcggggttac
     8401 cgaaactggt cacttctctt gacgcgattt tgtcgtcagc tacgacagca taaaagcgac
     8461 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
     8521 aaccaataaa ccaatttctg tgcagccaaa aatgacaccc tgcgccccct gttctgcaag
     8581 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
     8641 ttcttcaaaa ataatctgat taatcttcgc ccgttcatcc gcttccggaa taagacaatt
     8701 gatggaaaat tattccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
     8761 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
     8821 cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc gctgtaagcc
     8881 aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac gctggcactc
     8941 ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa gcccaccaag
     9001 ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt cccagctcat
     9061 tcctcccagc aaaccaattg ttttcattcc tctctctcct gatgtgtgtt accgcaacaa
     9121 ggctgaataa ggaacgagac aaacgcctca acggccaggt gcccaatctc tattaacgaa
     9181 aaaagggccg gatgtacagc acatccggcc cgtgaaatca gacgccgata tttctcaact
     9241 tctcgcctgc catcagtttg cgttcgatat gttccagcgt gacatttttg gtttccggaa
     9301 tgagccagaa agtgatgccc acaaacgcaa tgttcagcgc agtgtagagc cagaacgtac
     9361 cggcagcacc aatgctgtcg agcagcgtca ggaaggtcgc gccgataatc atattcgaca
     9421 cccagttcgt ggtggtcgaa caggtaatac cgaaatcgcg gcatttcagc ggctgaattt
     9481 cagagcacag gatccacacc actggcgcgg cgctcatcgc ataaccggca atacacatca
     9541 tcgtcatgcc aacagagagc caggacaagc cactggaagc cgtaccgtta tcaaactgca
     9601 tcaggcaata gcccagcacc agagtgccta acgccatcac gctgaaacca attttcagag
     9661 ccggtttacg ccctgcttta tctaccgtaa acaccgcaat aaaggtggcg aacataaagg
     9721 tcagccctac gaccagagtc gcaatcatct gttgttctgt ggtcgtaaag cccgccattt
     9781 tgaagatacg cggcgcgtag tacatgatga tgttcatacc ggtaaactgc tgcatcgcct
     9841 gcaacaacat accgagaaac acagcacgac ggacgttacg gttgatctta aacagtgccc
     9901 aaccgccctg ttttaacttc aggctttcac gaatttcgtt gagttcttct cgcgcttttt
     9961 ccgacgtatc gcgcagcata cgcaacactt cttccgcctc aatatgacgc cccttttccg
    10021 ccagccagcg cgggctattt ggcaggaaga ccaccagaat aatcagcagg actgctggta
    10081 aagcaagaac ccccaacatt gcgcgccagt taccgctata actgaacgct gtatcggata
    10141 aaaacgccag cacgatgccg agtgtgacca tcaactggta catactgatc atcttaccgc
    10201 gaacgttttc acttgccatt tcagaaagat acagaggagc ggtgtaagac gcgatcccga
    10261 cagcaatgcc cagcaccacc cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg
    10321 accctataga accgagtaca aacaggatgg cccccgccat caggctgtat ttacgcccca
    10381 ggcggaacga cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac
    10441 taaccaccca ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg
    10501 ctccggcgat tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg
    10561 aaacaaacat attcatacgc cgcgtatccc gcaaagaacg tggcgttaaa gcagattccg
    10621 tattgatagt aaccattttt tcctgccagc agagagtaag acatagtgaa aaaatacgtg
    10681 aacaactcac acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcg
//
LOCUS       DAZOYL010000127        10686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZOYL010000127 DAZOYL010000000
VERSION     DAZOYL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.127"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHUOY_002378"
     CDS             complement(<1..784)
                     /locus_tag="ACHUOY_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0683139.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHUOY_002379"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHUOY_002379"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR0683140.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHUOY_002380"
     CDS             1860..2747
                     /locus_tag="ACHUOY_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0683141.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHUOY_002381"
     CDS             2780..3790
                     /locus_tag="ACHUOY_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR0683142.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHUOY_002382"
     CDS             3860..5293
                     /locus_tag="ACHUOY_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0683143.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHUOY_002383"
     CDS             5392..6741
                     /locus_tag="ACHUOY_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0683144.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHUOY_002384"
     CDS             6731..7618
                     /locus_tag="ACHUOY_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0683145.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUOY_002385"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUOY_002385"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR0683146.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHUOY_002386"
     CDS             8352..9224
                     /locus_tag="ACHUOY_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR0683147.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUOY_002387"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUOY_002387"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR0683148.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUOY_002388"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUOY_002388"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR0683149.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2461 a         2532 c         2855 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccggg taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAZOYL010000128        10677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZOYL010000128 DAZOYL010000000
VERSION     DAZOYL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.128"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHUOY_002389"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHUOY_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR0683150.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="ACHUOY_002390"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="ACHUOY_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR0683151.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="ACHUOY_002391"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="ACHUOY_002391"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR0683152.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="ACHUOY_002392"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="ACHUOY_002392"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR0683153.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="ACHUOY_002393"
     CDS             complement(5254..6294)
                     /locus_tag="ACHUOY_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0683154.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="ACHUOY_002394"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="ACHUOY_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR0683155.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHUOY_002395"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHUOY_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR0683156.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="ACHUOY_002396"
     CDS             7229..7669
                     /locus_tag="ACHUOY_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR0683157.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHUOY_002397"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHUOY_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR0683158.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHUOY_002398"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHUOY_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR0683159.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..>10677
                     /gene="rsxC"
                     /locus_tag="ACHUOY_002399"
     CDS             8898..>10677
                     /gene="rsxC"
                     /locus_tag="ACHUOY_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR0683160.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEA"
BASE COUNT         2456 a         2763 c         2729 g         2729 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggtcg atccgcgcaa agccgccgtc gaagccg
//
LOCUS       DAZOYL010000129        10598 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZOYL010000129 DAZOYL010000000
VERSION     DAZOYL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10598
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.129"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            113..409
                     /locus_tag="ACHUOY_002400"
     CDS             113..409
                     /locus_tag="ACHUOY_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR0683161.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(562..1338)
                     /gene="yaaA"
                     /locus_tag="ACHUOY_002401"
     CDS             complement(562..1338)
                     /gene="yaaA"
                     /locus_tag="ACHUOY_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR0683162.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1408..2837)
                     /locus_tag="ACHUOY_002402"
                     /pseudo
     CDS             complement(1408..2837)
                     /locus_tag="ACHUOY_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            3116..4069
                     /gene="tal"
                     /locus_tag="ACHUOY_002403"
     CDS             3116..4069
                     /gene="tal"
                     /locus_tag="ACHUOY_002403"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0683163.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            4184..4771
                     /gene="mog"
                     /locus_tag="ACHUOY_002404"
     CDS             4184..4771
                     /gene="mog"
                     /locus_tag="ACHUOY_002404"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR0683164.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4806..5372)
                     /gene="satP"
                     /locus_tag="ACHUOY_002405"
     CDS             complement(4806..5372)
                     /gene="satP"
                     /locus_tag="ACHUOY_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR0683165.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5521..6234)
                     /gene="msyB"
                     /locus_tag="ACHUOY_002406"
     CDS             complement(5521..6234)
                     /gene="msyB"
                     /locus_tag="ACHUOY_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR0683166.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            complement(6260..6664)
                     /locus_tag="ACHUOY_002407"
     CDS             complement(6260..6664)
                     /locus_tag="ACHUOY_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR0683167.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7041..8957
                     /gene="dnaK"
                     /locus_tag="ACHUOY_002408"
     CDS             7041..8957
                     /gene="dnaK"
                     /locus_tag="ACHUOY_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR0683168.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            9046..10176
                     /gene="dnaJ"
                     /locus_tag="ACHUOY_002409"
     CDS             9046..10176
                     /gene="dnaJ"
                     /locus_tag="ACHUOY_002409"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR0683169.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(10280..10432)
                     /gene="mokC"
                     /locus_tag="ACHUOY_002410"
     CDS             complement(10280..10432)
                     /gene="mokC"
                     /locus_tag="ACHUOY_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR0683170.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2799 a         2659 c         2738 g         2402 t
ORIGIN      
        1 tcaataaatg cggtaactta gagattagga ttgcggagaa taacaaccgt cgttctcatc
       61 gcgtaatctc cggatatcga cccataacgg gcaatgataa aaggagtaac ctgtgaaaaa
      121 gatgcaatct atcgtactcg cactttccct ggttctggtc gctcccatgg cagcacaggc
      181 tgcggaaatt acgttagtcc cgtcagtaaa attacagata ggcgatcgtg ataatcgtgg
      241 ctattactgg gatggaggtc actggcgcga ccacggctgg tggaaacaac attatgaatg
      301 gcgaggcaat cgctggcacc cacatggacc gccgccaccg ccgcgccacc ataagaaagc
      361 tcctcatgat catcacggcg gtcatggtcc aggcaaacat caccgctaaa tgacaaatgc
      421 cgggtaacaa tccggcattc agcgcctgat gcgacgctgg cgcgtcttat caggcctacg
      481 tgaattctgc aatatattga atctgcatgc ttttgtaggc cggataaggc gttcacgccg
      541 catccggcat tgactgcaaa cttaacgctg ctcgtagcgt ttaaacacca gttcgccatt
      601 gctggaggaa gcttcatcaa agaagtaacc ttcgctatta aaaccagtca gttgctctgg
      661 tttggtcagc cgattttcaa taatgaaacg actcatcaga ccgcgtgctt tcttagcgta
      721 gaagctgatt atcttaaatt tgccgttctt ctcatcgagg aacaccggct tgataatctc
      781 ggcattcaat ttcttcggct tcaccgattt aaaatactca tctgacgcca gattaatcac
      841 cacattatcg ccttgtgctg cgagcgcctc gttcagcttg ttggtgatga tatctcccca
      901 gaattgatac agatctttcc ctcgggcatt ctcaagacgg atccccattt ccagacgata
      961 aggctgcatt aaatcgagcg ggcgcagtac gccatacaag ccggaaagca ttcgcaaatg
     1021 ctgttgggca aaatcgaaat cgtcttcgct gaaagtttcg gcctgcaagc cggtgtagac
     1081 atcaccttta aacgccaaaa tagcctggcg ggcattctcc ggcgtgaaat ctggctgcca
     1141 gtcatgaaag cgagcggcgt tgatacccgc cagtttgtcg ctgatgcgca tcagcgtgct
     1201 aatctgcgga ggcgtcagtt tccgcgcttc atggatcaag tgctgggaat tgtctaacag
     1261 ctccggcagc gtatagcgcg tggtggtcaa cgggctttgg taatcaagcg ttttcgcagg
     1321 tgaaataaga atcagcatat ccagtccttg caggaaattt atgccgactt tagcaaaaaa
     1381 agagaatgag ttgatcgata gttgtgatta ctcctgcgaa acatcatccc acgcgtccgg
     1441 agaaagctgg cgaccgatat ccggataacg caacggatca aacaccgggc gcacgccgag
     1501 tttacgctgg cgtagataat cactggcaat ggtatgaacc acaggcgaga gcagtaaaat
     1561 ggcggtcaaa ttggtaatag ccatgcaggc cattatgata tctgccagtt gccacatcag
     1621 cggaagactt agcaaggtgc cgccgatgac cgttgcgaag gtgcagatcc gcaaacacca
     1681 gatcgcttta gggttgttca ggcgtaaaaa gaagagattg ttttcggcat aaatgtagtt
     1741 ggcaacgatg gagctgaagg caaacagaat aaccacgagg gtaacaaact cagcacccca
     1801 ggaacccatt agcacccgca tcgccttctg gataagctga ataccttcca gcggcatgta
     1861 ggttgtgtcg ttacccgcca gtaatatcag catggcgctt gccgtacaga tgaccagggt
     1921 gtcgataaaa atgccaatca tctggacaat cccttgcgct gccggatgcg gaggccagga
     1981 cgccgctgcc gctgccgcgt ttggcgtcga acccattccc gcctcattgg aaaacatact
     2041 gcgctgaaaa ccgttagtaa tcgcctggct taaggtatat cccgccgcgc cgcctgccgc
     2101 ttcctgccag ccaaaagcac tctcaaaaat agaccaaatg acgtggggaa gttgcccgat
     2161 atttattacg caaatcacca tgctggtcag cacccagatt atcgccatca acgggacaaa
     2221 cccctgcatg agccgggcga cgccatgaag accgcgagtg attgccagca gagcaaagac
     2281 agcgagaata atgcctgtca ccagcggggg aaaatcaaaa gaaaactcag ggctcgggca
     2341 acggcgttcg cctgaactcc gctgaaaatt atgccgtagg cgatgagcaa aaagacggcg
     2401 aacagaacgc ccatccaacg catccccagc ccgcgcgcca tataccatgc cggtcctcca
     2461 cgaaactgtc cattgacgtc acgttcttta taaagttgtg caagggaaca ttcggcaaac
     2521 gaggtcgcca tgccgataaa cgcggcaacc cacatccaaa agacggctcc aggtccaccg
     2581 gcggtaatag ccagcgcaac gccggccagg ttgccgctac ccacgcgcgc cgcaagactg
     2641 gtacacaatg actgaaatga ggttaaaccg cctggctgtg gatgaatgct atttttaaga
     2701 cttttgccaa actggcggat gtagcgaaac tgcacaaatc cggtgcgaaa agtgaaccaa
     2761 caacctgcgc cgaagagcag gtaaatcatt accgatcccc aaaggacgct gttaataaag
     2821 gagaaaaaat ctggcatgca tatccctctt attgccggtc gcgatgactt tcctgtgtaa
     2881 acgttaccaa ttgtttaaga agtatatacg ctacgaggta cttgataact tctgcgtagc
     2941 atacatgagg ttttgtataa aaatggcggg cgatatcaac gcagtgtcag aaatccgaaa
     3001 cagtctcgcc tggcgataac cgtcttgtcg gcggttgcgc tgacgttgcg tcgtgatatc
     3061 atcagggcag accggttaca tccccctaac aagctgttta aagagaaata ctatcatgac
     3121 ggacaaattg acctcccttc gtcagtacac caccgtagtg gccgacactg gggacatcgc
     3181 ggcaatgaag ctgtatcaac cgcaggatgc cacaaccaac ccttctctca ttcttaacgc
     3241 agcgcagatt ccggaatacc gtaagttgat tgatgatgct gtcgcctggg cgaaacagca
     3301 gagcaacgat cgcgcgcagc agatcgtgga cgcgaccgac aaactggcag taaatattgg
     3361 tctggaaatc ctgaaactgg ttccgggccg tatctcaact gaagttgatg cgcgtctttc
     3421 ctatgacacc gaagcgtcaa ttgcgaaagc aaaacgcctg atcaaactct acaacgatgc
     3481 tggtattagc aacgatcgta ttctgatcaa actggcttct acttggcagg gtatccgtgc
     3541 tgcagaacag ctggaaaaag aaggcatcaa ctgtaacctg accctgctgt tctccttcgc
     3601 tcaggctcgt gcttgtgcgg aagcaggcgt gttcctgatc tcgccatttg ttggccgtat
     3661 tcttgactgg tacaaagcga atactgataa gaaagagtac gctccggcag aagatccggg
     3721 cgtggtttct gtatctgaaa tctaccagta ctacaaagag cacggttatg aaaccgtggt
     3781 tatgggcgca agcttccgta acatcggcga aattctggaa ctggcaggct gcgaccgtct
     3841 taccatcgca ccggcactgc tgaaagagct ggctgagagc gaaggggcta tcgaacgtaa
     3901 actgtcttac accggcgaag tgaaagcgcg tccggcgcgt atcactgagt ccgagttcct
     3961 gtggcagcac aaccaggatc caatggcagt agataaactg gcggaaggta tccgtaagtt
     4021 tgctattgac caggaaaaac tggaaaaaat gatcggcgat ctgctgtaat cattcttagc
     4081 gtgaccggga agtcggtcac gctacctctt ctgaagcctg tctgtcactc ccttcgcagt
     4141 gtatcattct gtttaacgag actgtttaaa cggaaaaatc ttgatgaata ctttacgtat
     4201 tggcttagtt tccatctctg atcgcgcatc cagcggcgtt tatcaggata aaggcatccc
     4261 tgcgctggaa gaatggctga catcggcgct aaccacgccg tttgaactgg aaacccgctt
     4321 aatccccgat gagcaggcga tcatcgagca aacgttgtgt gagctagtgg atgaaatgag
     4381 ttgccatctg gtgctcacca cgggcggaac tggcccggcg cgtcgtgacg taacgcccga
     4441 tgcgacgctg gcagtagcgg accgcgagat gcctggcttt ggtgaacaga tgcgccagat
     4501 aagcctgcat tttgtaccaa ctgcgatcct ttcgcgtcag gttggtgtga ttcgcaaaca
     4561 ggcgctgatc cttaacttac ccggtcagcc gaagtctatt aaagagacgc tggaaggtgt
     4621 gaaggacgct gcgggtaacg ttgtggtaca cggtattttt gccagcgtac cgtactgcat
     4681 tcagttgctg gaagggccat acgttgaaac ggcaccagaa gtggttgctg cattcagacc
     4741 gaagagtgca agacgcgacg ttagcgaata aaaaaatccc cccgagcggg gggatctcaa
     4801 aacaattagt gggattcacc aatcggcaga acggtgcgac caaactgctc gttcagtact
     4861 tcacccatcg ccagatagat tgcactggca ccacagatca gaccaatcca gccggcaaag
     4921 tggatgattg cggcgttacc ggcaatgtta ccgatcgcca gcagggcaaa cagcacagtc
     4981 aggctaaaga aaacgaattg cagaacgcgt gcgcctttca gcgtgccgaa gaacataaac
     5041 agcgtaaata cgccccacag acccaggtag acaccaagga actgtgcatt tggcgcatcg
     5101 gtcagaccca gtttcggcat cagcagaatc gcaaccagcg tcaaccagaa agaaccgtaa
     5161 gaggtgaatg cggttaaacc gaaagtgttg ccttttttgt actccagcag accggcaaaa
     5221 atttgcgcga tgccgccgta gaaaatgccc atggcaagaa taataccgtc cagagcgaaa
     5281 taacccacgt tgtgcaggtt aagcagaatg gtggtcatgc cgaagcccat caggcccagc
     5341 ggtgccggat tagccaactt agtgttgccc ataattcctc aaaaatcatc atcgaatgaa
     5401 tggtgaaata atttccctga ataacagtag tgttttcagg gcgcggcata ataatcagcc
     5461 agtggggcag tgtctacgat cttttgaggg gaaaatgaaa attttccccg gtttacggtg
     5521 tcagacctga gtggcgctaa ccatccggcg caggcaggcg atttgtatta cggctggaat
     5581 tgtcacgcga taggcactgc cgctgaccgc tttaacccca tttaatgctg cgcccacagg
     5641 gcctcccaga cccgcgccgc gcagcaaacc atgcccaagc acgctcattg ctgcgtgggt
     5701 gcgtaaaatg cgggtcaatt ggctggaaag caaatgcgac acgccttttg ccaataattt
     5761 gtctttcatc agcagcggca gcagctcttc cagctcattc accctggcat cgaccgcgtg
     5821 cagaaactcc tgcttatgtt cctcgtccat attcttccag gtgttacgca gaaattgttc
     5881 cagtaactgt tgctcaatct caaacgtaga catctctttg tcggctttca gcttcaatcg
     5941 ttttgaaaca tcgagcaaaa tggcccgata caatttaccg tgtccgcgca gtttgttggc
     6001 gatactatcg ccaccaaaat gctgtaattc tccagcaatc agctgccagt tgcggcgatg
     6061 ttgctcggga tgcccttcca tcgatttaaa cagttcgttg cgcatcagta cgctggagag
     6121 gcgagttttg cctttttcat tatgggtgag caatcgggcg aaatttgcca actgttcctc
     6181 actacaatgc tgaagaaaat ccagatctga atcattcagg taattaacat tcattttttg
     6241 tggtttctat attctggcgt tagtcgtcgc cgataatttt cagcgtggcc atatccgatg
     6301 agttcaccgt atgaccggaa aaggtgattt ttgagacgca gcgtttattg tcgttatcac
     6361 tgttaatgtt gatccagtca gtggtttgcc cttcttttat ttctgaagga atattcagac
     6421 tctgactggc gctacgggcg gctttgaaat aaaccgatgc accgcttaac tgtaaatcgc
     6481 catggtcggc agagagttgt atgcgtttca caatgcgaca aacaggaagt ttcagcgcca
     6541 gatcgttggt ttcgttacgc ggcattgcaa tgacgccgag gagtttatgg tcgtttgcct
     6601 gcgccgtgca gcacagcatc aggctaatcg ccaggctggc ggaaatcgta aaaacggatt
     6661 tcataaggat tctcttagtg ggaagaggta gagggatgaa tacccactag tttactgctg
     6721 ataaagagaa gattcaggta cgtaatcttt tctttttatt acaatttttt gatgaatgcc
     6781 ttggctgcga ttcattcttt atatgaataa aattgctgtc aattttacgt cttgtcctgc
     6841 catatcgcga aatttctgcg caaaagcaca aaaaattttt gcatctcccc cttgatgacg
     6901 tgggttacga ccccatttag tagtcaaccg cagtgagtga gtctgcaaaa aaatgaaatt
     6961 gggcagttga aaccagacgt ttcgccccta ttacagactc acaaccacat gatgaccgaa
     7021 tatatagtgg agacgtttag atgggtaaaa taattggtat cgacctgggt actaccaact
     7081 cttgtgtagc gattatggat ggcaccactc ctcgcgtgct ggagaacgcc gaaggcgatc
     7141 gcaccacgcc ttctattatt gcctataccc aggatggtga aactctggtt ggtcagccgg
     7201 ctaaacgtca ggcagtgacg aacccgcaaa acaccctgtt tgcgattaaa cgcctgattg
     7261 gccgccgctt ccaggacgaa gaagtacagc gtgatgtttc catcatgccg ttcaaaatta
     7321 ttgctgctga taacggcgac gcatgggtcg aagttaaagg ccagaaaatg gcaccgccgc
     7381 agatttctgc tgaagtgctg aaaaaaatga agaaaaccgc tgaagattac ttgggtgaac
     7441 cggtaactga agctgttatc accgtaccgg catactttaa cgatgctcag cgtcaggcaa
     7501 ccaaagacgc aggccgtatc gctggtctgg aagtaaaacg tatcatcaac gaaccgaccg
     7561 cagctgcgct ggcttacggt ctggacaaag gtactggcaa ccgtactatc gcggtttatg
     7621 acctgggtgg tggtactttc gatatttcta ttatcgaaat cgacgaagtt gacggcgaaa
     7681 aaaccttcga agttctggct accaacggtg atacccacct ggggggtgaa gacttcgaca
     7741 gccgtctgat caactacctg gttgaagaat tcaagaaaga tcagggcatt gacctgcgca
     7801 acgatccgct ggcaatgcag cgcctgaaag aagcggcaga aaaagcgaaa atcgaactgt
     7861 cttccgctca gcagaccgac gttaacctgc cgtacatcac tgcagatgcg accggtccga
     7921 aacacatgaa catcaaagtg actcgtgcga aactggaaag cctggttgaa gatctggtaa
     7981 accgttccat tgagccgctg aaagttgcac tgcaggacgc tggcctgtcc gtatctgata
     8041 tcgacgacgt tatcctcgtg ggtggtcaga ctcgtatgcc aatggttcag aagaaagttg
     8101 ctgagttctt tggtaaagag ccgcgtaaag acgttaaccc ggacgaagct gtagcaatcg
     8161 gtgctgctgt tcagggtggt gttctgactg gtgacgtaaa agacgtactg ctgctggacg
     8221 ttaccccgct gtctctgggt atcgaaacca tgggcggtgt gatgacgacg ctgatcgcga
     8281 aaaataccac tatcccgacc aagcacagcc aggtgttctc taccgctgaa gacaaccagt
     8341 ctgcggtaac catccatgtg ctgcagggtg aacgtaaacg tgcagctgat aacaaatctc
     8401 tgggtcagtt caacctggat ggtatcaacc cggcaccgcg cggcatgccg cagatcgaag
     8461 ttaccttcga tatcgatgct gacggtatct tgcacgtttc cgcgaaagat aaaaacagcg
     8521 gtaaagagca gaagatcacc atcaaggctt cttctggtct gaacgaagat gaaatccaga
     8581 aaatggtacg cgacgcagaa gctaacgccg aagctgaccg taagtttgaa gagctggtac
     8641 agactcgcaa ccagggcgac catctgctgc acagcacccg taagcaggtt gaagaagcag
     8701 gcgacaaact gccggctgac gacaaaactg ctatcgagtc tgcgctgact gcactggaaa
     8761 ctgctctgaa aggtgaagac aaagccgcta tcgaagcgaa aatgcaggaa ctggcacagg
     8821 tttcccagaa actgatggaa atcgcccagc agcaacatgc ccagcagcag actgccggtg
     8881 ctgatgcttc tgcaaacaac gcgaaagatg acgatgttgt cgacgctgaa tttgaagaag
     8941 tcaaagacaa aaaataatcg ccctataaac gggtaattat actgacacgg gcgaagggga
     9001 atttcctctc cgcccgtgca ttcatctagg ggcaatttaa aaaagatggc taagcaagat
     9061 tattacgaga ttttaggcgt ttccaaaaca gcggaagagc gtgaaatcaa aaaggcctac
     9121 aaacgcctgg ccatgaaata ccacccggac cgtaaccagg gtgacaaaga ggccgaggcg
     9181 aaatttaaag agatcaagga agcttatgaa gttctgaccg actcgcaaaa acgtgcggca
     9241 tacgatcagt atggtcatgc tgcgtttgag caaggtggca tgggcggcgg cggttttggc
     9301 ggcggcgcag acttcagcga tatttttggt gacgttttcg gcgatatttt tggcggcgga
     9361 cgtggtcgtc aacgtgcggc gcgcggtgct gatttacgct ataacatgga gctcaccctc
     9421 gaagaagctg tacgtggcgt gaccaaagag atccgcattc cgactctgga agagtgtgac
     9481 gtttgccacg gtagcggtgc aaaaccaggt acacagccgc agacctgtcc gacctgtcat
     9541 ggttctggtc aggtgcagat gcgccaggga tttttcgctg tacagcagac ctgtccacac
     9601 tgtcagggcc gcggtacgct gatcaaagat ccgtgcaaca aatgtcatgg tcatggtcgt
     9661 gttgagcgca gcaaaacgct gtccgttaaa atcccggcag gggtggacac tggagaccgc
     9721 atccgtcttg cgggcgaagg tgaagcgggc gagcatggcg caccggcagg cgatctgtac
     9781 gttcaggttc aggttaaaca gcacccgatt ttcgagcgtg aaggcaacaa cctctattgc
     9841 gaagtcccga tcaacttcgc tatggcggcg ctgggtggcg aaatcgaagt accgaccctt
     9901 gatggtcgcg tcaaactgaa agtgcctggc gaaacccaga ccggtaagct tttccgtatg
     9961 cgcggtaaag gcgtcaagtc tgtccgcggt ggcgcacagg gtgatttgct gtgccgcgtt
    10021 gtcgtcgaaa caccggtagg cctgaacgag aagcagaaac agttgctgca agagctgcaa
    10081 gaaagcttcg gtggcccaac cggtgagcac aacagcccgc gctcaaagag cttctttgat
    10141 ggtgtgaaga agttttttga cgacctgacc cgctaacctc cccaaaagcc tgcccgtggg
    10201 caggcttggg taaaattagg gtgcgttgaa gatatgcgag cacctgaaaa gtggcgggga
    10261 tcactccccg ccgttgctct tactcggatt cgtaagccgt gaaaacagca acctccgtct
    10321 ggccagttcg gatgtgaacc tcacagaggt cttttctcgt taccagcgcc gccactacgg
    10381 cggtgataca gatgacgatc agggcgacaa tcatcgcctt atgctgcttc attgctctct
    10441 tctccttgac cttacggtca gtaagaggca ctctacatgt gttcagcata tagggggcct
    10501 cgggttgata gtaaaatatc actcggggct tttctctatc tgccgttcag ctaatgcctg
    10561 agacagatag cctcaagcac ccgccgctat tatatcgc
//
LOCUS       DAZOYL010000130        10436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZOYL010000130 DAZOYL010000000
VERSION     DAZOYL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.130"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            109..684
                     /gene="ssuE"
                     /locus_tag="ACHUOY_002411"
     CDS             109..684
                     /gene="ssuE"
                     /locus_tag="ACHUOY_002411"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR0683171.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            677..1636
                     /gene="ssuA"
                     /locus_tag="ACHUOY_002412"
     CDS             677..1636
                     /gene="ssuA"
                     /locus_tag="ACHUOY_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR0683172.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1633..2780
                     /gene="ssuD"
                     /locus_tag="ACHUOY_002413"
                     /pseudo
     CDS             1633..2780
                     /gene="ssuD"
                     /locus_tag="ACHUOY_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2792..3583
                     /gene="ssuC"
                     /locus_tag="ACHUOY_002414"
     CDS             2792..3583
                     /gene="ssuC"
                     /locus_tag="ACHUOY_002414"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR0683173.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3580..4347
                     /gene="ssuB"
                     /locus_tag="ACHUOY_002415"
     CDS             3580..4347
                     /gene="ssuB"
                     /locus_tag="ACHUOY_002415"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0683174.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4554..7166)
                     /gene="pepN"
                     /locus_tag="ACHUOY_002416"
     CDS             complement(4554..7166)
                     /gene="pepN"
                     /locus_tag="ACHUOY_002416"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR0683175.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7432..8634
                     /gene="pncB"
                     /locus_tag="ACHUOY_002417"
     CDS             7432..8634
                     /gene="pncB"
                     /locus_tag="ACHUOY_002417"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR0683176.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8807..10207
                     /gene="asnS"
                     /locus_tag="ACHUOY_002418"
     CDS             8807..10207
                     /gene="asnS"
                     /locus_tag="ACHUOY_002418"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR0683177.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2348 a         2691 c         2819 g         2578 t
ORIGIN      
        1 aaaaagatac acaaaattta tatttggcat tttcttgtct ctccgacaga ctgaccgaat
       61 tgttatcaat gttaacaaaa aaagaacaat tggttataag gagagagtat gcgtgtcatc
      121 accctggcgg gtagtcctcg ctttccttct cgctccagct ccttgctgga atatgcgcgg
      181 gaaaaactaa atggcctgga tgtagaggtt tatcactgga atctgcaaaa cttcgccccg
      241 gaagatctgc tttacgctcg tttcgatagt ccggcactca agaccttcac cgaacagctg
      301 caacaggctg atggactgat agtcgctaca cctgtgtata aagccgccta ttccggcgcg
      361 ctgaaaaccc tgctcgacct gctgccagag cgcgctttgc aaggcaaagt ggtgctaccg
      421 ctggcgacgg gcggtacggt ggctcatctg ctggcggtcg attatgccct taagccagtt
      481 ttaagtgcga tgaaagccca ggagctcctg cacggcgtgt ttgccgatga ctcacaagtt
      541 attgattacc atcacaaacc ccagttcacg ccaaatctgc aaacccgtct tgataccgcg
      601 ctagaaactt tctggcaggc attgcaccgc cgcgatgttc aggttcctga ccttctgtct
      661 ctgcgaggta atgtccatgc gtaacatcat taaactggcg ctggcgggat tgcttagcgt
      721 ctctactttt gcggttgctg cagaatcctc gcctgaagcg ttacgtatag gctatcagaa
      781 aggcagtatt ggtatggtat tggcaaaaag ccaccagtta ctggaaaaac gctatccgca
      841 atcaaaaatc tcctgggtgg agttccccgc gggtccgcaa atgttggaag cgttaaacgt
      901 tggcagtatt gatctcggca gtaccgggga tattccgcca atctttgccc aggctgccgg
      961 ggctgatttg atatatgtgg gcgtcgagcc accgaagccc aaagccgaag tgattctggt
     1021 ggcagaaaac agcccgatca aaaccgtagc cgatcttaaa ggtcacaaag ttgcctttca
     1081 gaaaggttcc agttcacaca accttttact gcgtgcactg cgtcaggccg gacttaagtt
     1141 tactgacatc cagcccactt atctgacgcc agctgatgcc cgcgccgcgt tccagcaagg
     1201 taacgttgac gcctgggcta tctgggatcc ctactactcc gctgcgttat tacagggcgg
     1261 cgtgcgggtg ctgaaagacg gcaccgatct caatcaaact ggatcgtttt atctggcagc
     1321 tcgcccctat gcagaaaaaa acggcgcttt tattcagggc gtactggcaa cctttagtga
     1381 ggccgatgcg ttaacccgca gccagcgcga gcaaagcatc gctttactgg caaaaacgat
     1441 gggcttaccg gcaccggtga ttgcctcgta tctggatcat cgtcctccca ccaccatcaa
     1501 accggtgagc gctgaagttg ccgccttaca gcagcaaacg gcagatctgt tttatgaaaa
     1561 ccgtctggtg tcgaaaaaag ttgatattcg ccagcgcatc tggcagccca ctcacctgga
     1621 aggaaaacaa ttatgagtct gaatatgttc tggtttttac cgacccatgg tgacgggcat
     1681 tatcttggaa cggaagaagg ttcacgcccg gttgatcacg gctatctgca acaaattgcg
     1741 caagcggcgg atcgtctggg ctataccggt gtgctgatcc caacggggcg atcgtgtgaa
     1801 gatgcgtggc tggtggcggc gtcgatgatc ccggtgacgc agcggctgaa gtttcttgtc
     1861 gccctgcgtc ccagcgtaac ctcacctacc gttgccgctc gccaggccgc cacgcttgac
     1921 cgtctctcaa atggtcgtgc gttgtttaat ctggtcacag gcagcgatcc acaagagttg
     1981 gcaggcgacg gcgtgttcct tgatcatagc gagcgctacg aagcctcggc ggaatttacc
     2041 caggtctggc ggggcgctta ttgctggggg aaagcgtcga tttcaacggt aaacatattc
     2101 atgtgcgggg agcaaaactg ctcttcccgc cgattcaaca gccgtatcct ccgctttact
     2161 ttggcggatc gtcagatgtc gcccaggagc tggcggcaga acaagttgat ctctacctca
     2221 cctggggcga accgccagag ctggtaaaag agaagatcga acaagtgcgg gcgaaagctg
     2281 ccgcgcatgg acgcaaaatt cgtttcggta ttcgtctgca tgtgattgtt cgtgaaacta
     2341 acgacgaagc gtggcaggcc gccgagcggt taatctcgca tcttgatgat gaaactatcg
     2401 ccaaagcaca ggccgcattc gcccggacgg attccgtagg acaacagcga atggcggcgc
     2461 tacataacgg caagcgcgac aatctggaga tcagccccaa tttatgggcg ggcgttggct
     2521 tagtgcgcgg cggtgccgga acggcgctgg tgggcgatgg tcctacggtc gccgcgcgaa
     2581 tcaacgaata cgccgcgctt ggcatcgaca gctttgtgct ttcgggctac ccgcatctgg
     2641 aagaagcgta tcgggttggc gagttgctgt tcccacatct ggatgtcgcc attccggaaa
     2701 ttccccagcc gcagccgctg aatccgcaag gcgaagcggt ggcgaatgat tttatccccc
     2761 gtaaagtcgc gcaaagctaa ggaacacgat gatggcaacg ccagtgaaga agtggttatt
     2821 gcgcgttgcc ccctggtttt taccggtggg catcgtggcg gtgtggcaac tggcctcctc
     2881 ggttggctgg ctgtcgacgc gtattttgcc ttcaccggaa ggagtggtga cggcgttctg
     2941 gacgctctcc gccagcggcg aattgtggca gcatctggcg atcagttcct ggcgggcgct
     3001 gattggcttt tcaattggcg gatcgctggg cttgattctg ggactgatta gcgggctgtc
     3061 acgctgggga gaacggctgc tggatacctc aattcagatg ttgcgcaacg taccgcatct
     3121 ggcgctgatc ccgctggtga ttttgtggtt tggcattgat gaatccgcaa aaatctttct
     3181 ggtggcgctc ggcacgctat tccccattta tatcaacacc tggcatggca tccgtaatat
     3241 cgatcgcggt ctggtggaaa tggcgcgtag ctatggatta tccggcatac cgctgtttat
     3301 ccatgtgatc ctgcctggtg ccctgccctc aattatggtc ggcgtgcgtt ttgcgttggg
     3361 cctgatgtgg ctgacgctga ttgttgccga aaccatttct gccaattcag gcattggtta
     3421 tctggcgatg aatgcgcggg agtttttgca aacggacgtg gtggtagtcg ctattattct
     3481 ttacgccctg ctcggcaaac ttgctgacgt cagcgcgcag ctgttagagc gcctctggtt
     3541 gcgctggaac ccggcttatc atttgaagga ggccacggta tgaatactgc tcgtctgaac
     3601 cagggcacac cattgttgct caatgcagta agcaaacatt acgcggaaaa tatcgtcctg
     3661 aaccaactgg atttacatat tccggcaggt cagtttgtgg cggtggtggg ccgcagcggt
     3721 ggtggcaaaa gtaccctgct gcgcctgctg gcaggtctgg aaacgccaac cgcaggcgat
     3781 gtgttagcgg gcaccacacc gctggctgag attcaggaag acacgcgaat gatgtttcag
     3841 gatgcgcgtc tgctgccgtg gaaatcggtg attgataacg ttgggttagg ccttaaaggc
     3901 cagtggcgcg atgccgcgcg tcgggcgttg gctgcagtag gactggagaa tcgcgccggg
     3961 gaatggcctg ccgcactttc tggcgggcag aaacagcgtg tggcgctggc aagagcgttg
     4021 attcatcgac cgggtttatt gttgcttgat gaaccgctcg gggcactgga cgccttaacg
     4081 cgactcgaga tgcaggattt gattgtgtca ctttggcagc agcacggctt taccgtactg
     4141 ttggtgacgc atgatgtgag cgaagcggta gcaatggctg accgggtgtt gttaattgaa
     4201 gagggaaaaa ttggtctgga tttgacagtg gatatccccc gtccgcgtcg attagggtcg
     4261 gtgcgattag cggagctgga agcggaagta ttgcaacggg taatgcggcg tgggcattct
     4321 gaacaactga ttcggcgtca tggttaatat gccggatgtg gcgcacaaaa ccagcatttt
     4381 caacggactt gtaggcctga taagacacgt caagcatcgc atcaggcagt tgggcacggc
     4441 tgccggatgc ggcgtgaacg ccttatccag cctacaaaac cagcaatttc aacggagttg
     4501 taggcctgat aagacgcgtc aagcgtcgca tcaggcattt tggctcaaaa gtatcaagcc
     4561 agtgctttgg tgatcttctc gtacagatcg ccagagagat tttccagccc tttcaactgt
     4621 tccagcgccg cgcgcatttt ctcctgacgt ttggcatcat aacgtttcag gcgaatcagc
     4681 ggttcaatca gacgtgaagc tacctgcggg ttacggctgt tgaggtcggt aagcatttcc
     4741 tccaggaact ggtaaccgct gccatcttcg gcatggaacg ctgccgggtt gctgcccgca
     4801 aacgcaccaa tcaacgaacg aatacggttc gggttgctca tggtaaatga gcgatgctgc
     4861 aacagaccac gcaccgtctc cagcacattc gccgccgggc tggtggcttg caggataaac
     4921 catttatcca tcaccagacc gtcctgatgc cacttatcgt cgtactcctg catcagcgcg
     4981 tcacggcaag gcagctgtgc agcaaccgcc gcagaaagcg ctgccagcgc atcggtcata
     5041 ttgtttgctt catggtattg cttgctcacc agcacgtcag ccagatgcgt ttcaccaaaa
     5101 gcaaggaagc gcaggcaggc atttcgcaga gtgcgttttg caatatcttc atgctcaaca
     5161 cggtactcgc tctggtaatt cgcgttgtaa atagccagca gctcatccgc cagttcagtc
     5221 gccagagtac gagtgagtgc ttcgcgtact tcggcaatag caatcgggtc gatgatatcg
     5281 aacagttcag ccatttcatt gacagaaggc agcgtcagga tttctgccgc cagcgccgga
     5341 tcaatcttct catcgagcag caccgcgcgg aaagcgtcag ccacatgtac cggcagagac
     5401 agcggctgcc cttgctgatg acgggcgacg ttcagcttga tgtaggttgc cagcaaactt
     5461 tgcgccgcat cccaacggga gaaatcatta cgcgcatgac gcatcaggaa ggtcagttgc
     5521 tgatcactcc acttatattc cagtttcact ggcgcagaga attcgcacag cagcgcaggc
     5581 accggctgga agtagacatt atcaaaaaca aaggtctgtt ccgcctgggt gacgttcagc
     5641 acggaattca ccggatgacc gcctttctgc aacgggatca ctttgccttc gttgtcatac
     5701 aattcgatgg caaacggaat atgcagcggc tgtttttctg cctgatccgg cgtggctggc
     5761 gtgcgctggc tgatggtcag ggtgtactgc tcagtttccg gattgtagtc gtctttgacg
     5821 gtcacaatcg gcgtaccgga ctggctgtac caacggcgga aatgggagag atcaacattc
     5881 gacgcatctt ccatcgcctg cacaaagtcg tcacaggtcg ctgcgctacc atcatgacgc
     5941 tcgaaataga gttgcatccc tttctggaag ttttcttcgc ccagcagggt gtggatcata
     6001 cggatcactt ccgcgccctt ctcgtaaacg gtcagggtgt agaagttgtt catctcaatg
     6061 accatgtccg ggcggattgg gtgcgccatc gggctggcgt cttccgcaaa ctgcaatccg
     6121 cgcatggtgc gcacattatt gatgcggtta actgcgcggg aaccaaggtc agagctgaat
     6181 tcctgatcgc ggaagacggt taaaccttct ttcaggctga gctggaacca gtcgcggcag
     6241 gtcactcggt taccggtcca gttatggaaa tattcatggc cgataacgcg ttcaatatcg
     6301 aggtaatctt tgtcggtggc ggtgtcggtg cgggccagca catatttgga gttaaagata
     6361 ttcagaccct tattctccat tgcgcccata ttgaagaaat ccaccgcgac gatcatatag
     6421 atgtcgaggt catactccag gccgaagcgt tcttcatccc atttcattga gtttttcagc
     6481 gaggtcatcg cccacggcgc gcgatcaagg ttgccgcgat cgacgtacag ctccagcgcc
     6541 acttcgcgac cagaacgcgt ggtaaaggtg tcgcgcagta catcaaagtc gcctgccacc
     6601 agcgcaaaca ggtagcacgg tttcgggaac gggtcctgcc actgtaccca atggcgtccg
     6661 ttttccagtt caccttgcgc aacgcggtta ccgttggaaa gcaggaaggg atatttggtt
     6721 ttatcggcaa taattttggt ggtaaaacgc gccagcacgt ccgggcggtc gagataatac
     6781 gtaatatggc ggaaaccttc ggcttcacac tgggtgcaaa gcgcatcgcc tgactgataa
     6841 agcccttcca gcgcggtatt cgccgccggg cttatttcat taacgatctt aagcgtaaaa
     6901 tgctccggca aattactgat gaccagtgcg ccctcttctt ctttccaggc ggtccacggc
     6961 tcatcattaa tatgaacaga aaccagtttg aggtcttcgc cgttgagacg aagtggagca
     7021 tctgatgcac catgacggac agcctggctg accgcggtaa cgaccgtctt ttgcgcgtcg
     7081 aggtcaaagg tcaagtcaat atcagtaatc tggtaatctg gcgcacgata atcgtgacgg
     7141 tatttggctt gtggctgttg agtcataaat aacctttagc atcttttata gagtctggtg
     7201 ttcagtctat tcctgttgcg taaatcgcgc tatgcagaat cttcatcttt tcaggtacaa
     7261 acgcctttat tgctacattt ttataacata caccgcgtaa tgccatcgac cagaaaggtg
     7321 gcatatggtg tgatcggggt tcaataaatt gcgaaacaag gtatactcca gcagttcctg
     7381 aagatgttta ttgtactaaa cgctcctgta cgaggacgct actgcgcacc tatgacacaa
     7441 ttcgcttctc ctgttctgca ctcgttgctg gatacagatg cttataagtt gcatatgcag
     7501 caagccgtgt ttcatcacta ttacgatgtg catgtcgcgg cggagtttcg ttgccgaggt
     7561 gacgatctgc tgggtattta tgccgatgct attcgtgaac agattcaggc gatgcagcac
     7621 ctgcgcctgc aggatgatga atatcagtgg ctctctgccc tgcctttctt taaggccgac
     7681 tatcttaact ggttacgcga gttccgcttt aacccggaac aagtcaccgt gtccaacgat
     7741 aatggcaagc tggatattcg cttaagcggt ccgtggcgtg aagtcatcct ctgggaagtt
     7801 cctttgctgg cggttatcag tgaaatggta catcgctatc gctcaccgca ggccgacgtt
     7861 gcgcaagccc tcgacacgct ggaaagcaaa ttagtcgact tctcggcgtt aaccgccggt
     7921 cttgatatgt cgcgcttcca tctgatggat tttggcaccc gtcgccgttt ttctcgcgaa
     7981 gtacaagaaa ccatcgttaa gcgtctgcaa caggaatcct ggttcgtggg caccagcaac
     8041 tacgatctgg cgcgtcggct ttccctcacg ccgatgggaa cacaggcaca cgaatggttc
     8101 caggcacatc agcaaatcag cccggatcta gccaacagcc agcgagctgc acttgctgcc
     8161 tggctggaag agtatcccga ccaacttggc attgcattaa ccgactgcat cactatggat
     8221 gctttcctgc gtgatttcgg tgtcgagttc gctagtcggt atcagggcct gcgtcatgac
     8281 tctggcgacc cggttgaatg gggtgaaaaa gccattgcac attatgaaaa gctgggaatt
     8341 gatccacaga gtaaaacgct ggttttctct gacaatctgg atttacgcaa agcggttgag
     8401 ctataccgcc acttctcttc ccgcgtgcaa ttaagttttg gtattgggac tcgcctgacc
     8461 tgcgatatcc cccaggtaaa acccctgaat attgtgatta agttggtaga gtgtaacggt
     8521 aaaccggtgg cgaaactttc tgacagccct ggcaaaacta tctgccatga taaagcgttt
     8581 gttcgggcgc tgcgcaaagc gttcgacctt ccgcatatta aaaaagccag ttaatatcat
     8641 cagggagcta atcggctccc tttttttacc tttaattccg aaatctttcg ctgcatttgc
     8701 gaattctgct tgtctgattg cagatgccag gtaacatagg tatccccccc cccatttacg
     8761 ggtggacaag tgtttatttt ttccgactat taacagagag aatattatga gcgttgtgcc
     8821 tgtagccgac gtactccagg gccgcgtagc cgttgacagc gaagtcaccg tacgcggatg
     8881 ggtacgtacc cgccgagatt caaaagctgg catctccttc ctcgccgttt atgacggttc
     8941 ctgctttgat cctgtacagg ctgtcatcaa taattctctg cccaattaca atgaagacgt
     9001 cctgcgtctg accaccggct gctcggtcat tgtgacgggt aaagtcgtgg catcgccggg
     9061 ccaggggcaa caatttgaaa ttcaggccag caaggttgaa gttgctggtt gggttgaaga
     9121 tccagacact tacccgatgg cggcaaaacg ccacagcatt gagtatctgc gtgaagtcgc
     9181 tcacctgcgt ccgcgcacaa acctgattgg tgccgtcgcg cgcgttcgcc atacgctggc
     9241 gcaggcgctg catcgcttct ttaacgagca gggattcttc tgggtttcaa cgccactgat
     9301 taccgcatct gataccgaag gtgcaggcga aatgttccgc gtttctacgc tggatctgga
     9361 aaacctgccg cgtaacgatc agggcaaagt ggatttcgac aaagacttct ttggtaaaga
     9421 gtctttcctg accgtatctg gccagttgaa cggcgaaacc tacgcttgcg cattatccaa
     9481 aatttatacc ttcggcccga ctttccgtgc tgaaaactcc aacaccagcc gtcacctggc
     9541 ggaattctgg atgctggagc cggaagtggc gtttgctaac ctgaacgata tcgcgggtct
     9601 ggctgaagcc atgctgaaat atgtcttcaa agcggttctc gaagaacgcg cagacgacat
     9661 gaaattcttc gctgaacgcg tagataaaga tgccgtttca cgtctggaac gcttcattga
     9721 ggccgatttc gcgcaggtgg attacaccga cgcagtgacc attctcgaaa actgcggcag
     9781 gaagtttgaa aacccggttt actggggcgt cgatctctct tctgagcatg agcgttatct
     9841 ggcggaagaa cactttaaag caccggtagt ggttaaaaac tatccgaaag atattaaagc
     9901 gttctatatg cgcctcaacg aagacggtaa aaccgttgcg gctatggacg ttctggctcc
     9961 gggcatcggt gagatcattg gtggctccca gcgtgaagaa cgtctggacg ttctggacga
    10021 gcgtatgctg gaaatgggcc tgaataaaga agattactgg tggtatcgcg atctgcgtcg
    10081 ctacggtact gttccgcatt caggtttcgg tcttggtttt gaacgcctga ttgcttacgt
    10141 aactggcgtg caaaacgtgc gtgatgtgat tccgttccca cgtactccgc gcaacgccag
    10201 cttctgattt tcttcctcaa agagccagcg cctgctggct cttttttatc ctttctgtca
    10261 agttatctgt ttgttaagtc aagcaatcta tttgcaaccc cgccataacg atcatcctgt
    10321 tacggaatat tacattgcaa catttacgca caaaaaataa cccgcattct tattgcggat
    10381 tagttttttc ttagctgata gcacaatttt catactattt tttggcattc tggatg
//
LOCUS       DAZOYL010000131        10421 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZOYL010000131 DAZOYL010000000
VERSION     DAZOYL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.131"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            173..1642
                     /gene="dtpB"
                     /locus_tag="ACHUOY_002419"
     CDS             173..1642
                     /gene="dtpB"
                     /locus_tag="ACHUOY_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR0683178.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(1691..2443)
                     /gene="rsmJ"
                     /locus_tag="ACHUOY_002420"
     CDS             complement(1691..2443)
                     /gene="rsmJ"
                     /locus_tag="ACHUOY_002420"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR0683179.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(2451..4493)
                     /gene="prlC"
                     /locus_tag="ACHUOY_002421"
     CDS             complement(2451..4493)
                     /gene="prlC"
                     /locus_tag="ACHUOY_002421"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR0683180.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            4696..5538
                     /gene="rlmJ"
                     /locus_tag="ACHUOY_002422"
     CDS             4696..5538
                     /gene="rlmJ"
                     /locus_tag="ACHUOY_002422"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR0683181.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5610..6962
                     /gene="gorA"
                     /locus_tag="ACHUOY_002423"
     CDS             5610..6962
                     /gene="gorA"
                     /locus_tag="ACHUOY_002423"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR0683182.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7016..7099)
                     /gene="dinQ"
                     /locus_tag="ACHUOY_002424"
     CDS             complement(7016..7099)
                     /gene="dinQ"
                     /locus_tag="ACHUOY_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0683183.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            7127..7300
                     /locus_tag="ACHUOY_002425"
     CDS             7127..7300
                     /locus_tag="ACHUOY_002425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683184.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            7839..8192
                     /gene="arsR"
                     /locus_tag="ACHUOY_002426"
     CDS             7839..8192
                     /gene="arsR"
                     /locus_tag="ACHUOY_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR0683185.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            8246..9535
                     /gene="arsB"
                     /locus_tag="ACHUOY_002427"
     CDS             8246..9535
                     /gene="arsB"
                     /locus_tag="ACHUOY_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR0683186.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            9548..9973
                     /gene="arsC"
                     /locus_tag="ACHUOY_002428"
     CDS             9548..9973
                     /gene="arsC"
                     /locus_tag="ACHUOY_002428"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR0683187.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(10107..10403)
                     /locus_tag="ACHUOY_002429"
     CDS             complement(10107..10403)
                     /locus_tag="ACHUOY_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0683188.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
BASE COUNT         2342 a         2779 c         2697 g         2603 t
ORIGIN      
        1 acactatcat attttccatc attagtgtga tcagctggtt attttctgtt gtaatagtgt
       61 attaatctat tcaccgcatc aatattaaga atctctgaca gatgtaaact ttttcgcgcg
      121 ttatccctta cgcgttcata cttttcagga tggtattgga aggttaataa atatgaatac
      181 aacaacaccc atggggatgc tgcagcaacc tcgcccattt ttcatgatct tttttgtcga
      241 gttatgggag cgattcggct actacggcgt gcagggcgta ctggcggttt tcttcgttaa
      301 acagcttgga ttctcgcaag agcaggcttt tgtcactttt ggtgcttttg ctgcgctggt
      361 ctatggcctc atttccattg gcggctatgt cggcgaccac ctgctgggga ccaaacgcac
      421 cattgttctc ggagcacttg tgctggcgat tggctacttc atgaccggca tgtcgctact
      481 taagcctgac ctgattttca tcgccctggg gactatcgct gtcggtaacg gcctgtttaa
      541 agctaaccca gccagcttgc tttcgaagtg ctatccgccg aaagatccgc ggcttgatgg
      601 cgcattcacc ctgttctata tgtcgatcaa tatcggctcg ttgatagcgt tatcgctggc
      661 ccctgtgatc gctgatagat acggttattc agtcacctac aacctgtgcg gggcggggtt
      721 aattatcgca ttactggttt acatcgcctg tcgtggaatg gtgaaagaca ttggttctga
      781 acccgacttc aagccgatga gcttcagcaa actgttgtac gtattacttg gcagcgtggt
      841 gatgatcttc gtatgtgcat ggctgatgca caacgtagaa gtcgccaatc tggtgctgat
      901 tgttctctcc atcgtcgtca ccatcatctt ctttcgtcag gcattcaagc tggataaaac
      961 cgggcgcaat aaaatgtttg tcgcctttgt cctgatgctc gaagcggtgg tgttttacat
     1021 tctctacgcc cagatgccaa cgtcgctgaa cttctttgcc atcaacaacg tgcatcatga
     1081 aattctcggc ttttccataa acccggtcag cttccaggcg cttaacccgt tctgggtggt
     1141 actcgccagc ccaatactgg caggcattta cacgcatctg ggtaacaaag gcaaagacct
     1201 ctcgatgccg atgaaattta ctctcggcat gtttatgtgc tcactgggct ttttgacggc
     1261 ggcagctgcg ggaatgtggt ttgcggatgc acaagggctg acatcgccat ggtttatcgt
     1321 gctggtgtat ttattccaga gcctgggtga attgtttatt agcgcccttg gcctggcgat
     1381 gattgctgcc ctggtgccgc agcatttgat gggctttatt ctcgggatgt ggttcctgac
     1441 gcaggctgcc gcattcttgc tgggcggcta tgtggcaaca tttaccgcag tgccggacaa
     1501 cattaccgat ccgcttgaga cgttacccgt ctataccaac gtgtttggta agattggcct
     1561 ggttacgctg ggcgttgcag tagtgatgct gttgatggtg ccgtggctga aacgcatgat
     1621 tgcgacgccg gaaagccatt aattattctt gcagaaagca gggtagcgtt atcgctaccc
     1681 tgctttactt ttacacgggc gtacctgcat agatatcaaa gcgatgccct ttagtcacaa
     1741 ccgcgtttgg cgtggcgaca ttcgctagcg gtggcgcgta gtccgggcgc tttactacca
     1801 cgcgtttggt cgccagcaag cgtgcaggct ccagcaatcc atcggcatca agatccggcc
     1861 ccaccagcga ctgaaagaca cgcatctctt ttttcaccag cgcgcttttc tgcttatgcg
     1921 ggaacatcgg gtcgagatag accacctgcg ggcgtggggt aatatcggtt agcgccgtca
     1981 ggctggaggc gtgaattaac tgcaaacgct cctgcaacca gccgccgatt tccgcatccg
     2041 cataaccacg cgccaggccg tcgtcgagca gcgcggcaac aactggatta cgctccagca
     2101 tccgcacgcg gcaaccgact gaagccagca caaaggcatc gcgccccagt cctgcagtgg
     2161 catccaccac atccggcaaa taatcgcctt taatgcccac cgctttcgcc accgcctcac
     2221 cgcgaccacc gccgaatttg cgtcggtgcg ccatcgctcc gccaacaaaa tcaacaaaga
     2281 tgccgccaag ttttggctca tcacgcttgc gcaattccag atgttccggc gttaacacca
     2341 gcgccatcag gttgtcttca tcgtgctcca gcccccagcg ggccgccaga acagataagg
     2401 cgccgtctcc ggcgcctgtt tcatcaatta agcagatttt cactgaatga tcagccctta
     2461 atgccgtaat gctccagcat cgcatccagc tgcggttcac gaccacggaa gcgtttgaac
     2521 agatccatcg gctcttctga accgccacgg ctcagaatgt tgtcgaggaa cgactgcccg
     2581 gtttcacggt tgaaaatgcc ctcttcctca aagcgcgaga aagcatctgc cgccagtacg
     2641 tcagcccaca ggtagctgta gtaacctgcc gcataaccac cggcgaaaat atggctgaaa
     2701 gcgtgcggga aacggcccca ggacggagat ggcaccacgg caaccagttt cttgatttct
     2761 gccagagttt cgaggatttt tgccccctga tccgggcgga actcggcatg aaggcggaaa
     2821 tcaaacaggc cgaactccag ctgacgcaga ataaacagcg ccgcctggta gttcttcgcc
     2881 gccagcattt tatccagcaa ctctttcggc agcggttcgc cggtttcata gtgaccagag
     2941 ataaacgcca gcgcctccgg ctcccagcac cagttttcca taaactgact cggcagttcg
     3001 accgcatccc acggcacacc gctgataccg gaaacaccag cggtttcgat gcgggtcagc
     3061 atatggtgca ggccgtgacc gaactcgtgg aacagggtga tcacttcgtc gtgagtgaac
     3121 agcgccggtt taccatttac cgggcggttg aagttacaag tcaaatacgc gaccggtttt
     3181 tgcagagaac catcagcttt acgcatctgg cctacgcagt catccatcca cgccccgccg
     3241 cgcttgtttt cacgggcata cagatcgagg tagaagctgc cgcgcagttc gttattttcg
     3301 tcatacagtt cgaagaaacg tacatccgga tgccagacat caacctcttt acgctcttta
     3361 gcggtgatgc cgtaaatacg tttcaccact tcaaacaggc cgttaaccga tttgttttcc
     3421 gggaagtacg gacgcagctg ctcgtcgctg atgctgtaga ggtgctgttt ctgtttttcg
     3481 ctgtagtaag cgatatccca cggctgcaac tcatcgacac caaattcggc tttagcgaag
     3541 gcacgcagtt gctccagctc tttttcgcct tgcggacgcg cgcgttttgc cagatcggtt
     3601 aagaaatcca gcacctgctg cgggttttct gccattttag tggcaaggga tttaaaggcg
     3661 tagttttcaa agcccagcag ttgcgccagt tcgtgacgca gcgcgaggat ctcttccatc
     3721 accttgctgt tatcccactt accggcgttc gggccttgat cggaggcgcg ggtgctgtaa
     3781 gcgcgataca tctcttcgcg cagagcctgg ttgtcgcagt aggtcattac cggcaggtag
     3841 cttgggatat ccagcgtcag caggtagcct tccagctctt tcgcttcggc ctgggctttt
     3901 gccgcagcca gcgcgctttc tggcatcccc gccagccccg cttcgtcggt aacgagtttg
     3961 gtccagccca tcgtcgcatc gaggacgttg ttgctgtact ggttgcccag ttcagaaaga
     4021 cgggtagcaa tttcgccgta acgctgctgt ttctctttcg gcagacctat gccagagagt
     4081 tcaaaatcac gcagggcgtt atcaaccgct ttcttctgcg ccgtgttcag cgtggcgtaa
     4141 tgatcgccat cgcgcaggtc gcggtacgct ttatacagcc cttcatgttg ccctacccag
     4201 gtgctgtatt ccgacagcag cggcagggtt tgctcgtagg cttcacgcag ttccgggcta
     4261 tttttcaccg agttcaggtg gctgaccggg gagaagatac gccccaacac atcgtccact
     4321 tccgccaacg gctggcagag attttcccag gtgtacggtg ccccttgcgc tactacgcgc
     4381 gccacatttt cgcggcaatc gttcagcgcc tgagtcacgg ctggaacgac atgttccggg
     4441 agaattttag aaaacggagg caattcaaag ggagtcagta acggattcgt cattcgcgca
     4501 gtcctggtta aagaggttaa ggaagcgctc aacaggcgct ttacataatg tgtgtagcat
     4561 ggggttaagt gtagtgaatt tcaatgagaa acgttacgct ttcgcggcgg cggcctcttt
     4621 tcggtatact gtcctgatac gcttttgtgc gccccgaata cgggccgatt tttacttacc
     4681 ggaacacctt tacccatgct cagttatcgc cacagctttc acgctggcaa ccacgccgac
     4741 gtccttaaac ataccgttca gagcctgatc atcgagtcgc tgaaagagaa agataaaccg
     4801 tttctctatc tcgataccca cgcaggggcc gggcgttatc agttaggcag cgaacatgcc
     4861 gagcgtaccg gcgaatatct cgaaggtatc gcccgtatct ggcagcagga cgatttgccc
     4921 gcagaactgg aggcgtacat caatgtggta aaacacttca accgtagcgg tcagttgcgt
     4981 tactaccccg ggtcgccgtt gattgctcgc cagctactgc gcgaacaaga cagcctgcaa
     5041 ctgaccgaac tgcacccgag cgattacccg ttgctgcgtt ctgaatttca gaaagacagc
     5101 cgtgcgcgcg tcgaaaaagc cgacggtttc cagcagctta aggccaaact tccgccggtt
     5161 tcccgccgtg gtttaatcct tatcgacccg ccgtatgaaa tgaaaaccga ctatcaggcg
     5221 gtggtcagcg ggatagcaga aggttacaaa cgtttcgcca ctggtactta cgcactgtgg
     5281 tatccggtgg tgctgcgtca gcaaattaag cgcatgatcc acgatctgga agcgaccggt
     5341 attcgcaaaa ttctgcaaat tgaactggcg gtactgccag acagcgatcg ccgtggcatg
     5401 accgcttccg gcatgattgt gattaacccg ccgtggaaac tggaacagca gatgaataac
     5461 gtgctgccgt ggctgcacag caaactggtt ccggcaggca ccgggcacgc caccgtaagc
     5521 tggatcgtgc cggagtaatt gcagccattg ctggcaccta ttacgtctcg cgctacaatc
     5581 gcggtaatca acgataagga cactctgtca tgactaaaca ctatgattac atcgccatcg
     5641 gcggcggcag cggcggtatc gcctccatca accgcgcggc tatgtacggc cagaaatgcg
     5701 cactgattga agccaaagag ctgggcggca cctgcgtgaa cgtcggctgc gtgccgaaaa
     5761 aagtgatgtg gcacgcggcg caaatccgtg aagcgatcca tatgtacggc cctgattatg
     5821 gtttcgatac caccatcaac aagttcaact gggaaacgtt gatcgccagc cgtaccgcct
     5881 atatcgaccg tattcatact tcctatgaaa acgtgctcgg taaaaataac gttgatgtaa
     5941 tcaaaggctt tgcccgcttc gttgatgcca aaacgctgga ggtaaacggc gaaaccatca
     6001 cggccgatca tattctgatc gccacaggcg gtcgtccgag ccacccggat attccgggcg
     6061 tggaatacgg tattgattct gatggcttct tcgcccttcc tgctttgcca gagcgcgtgg
     6121 cggttgttgg cgcgggttac atcgccgttg agctggcggg cgtgattaac ggcctcggcg
     6181 cgaaaacgca tctgtttgtg cgtaaacatg cgccgctgcg cagcttcgac ccgatgattt
     6241 ccgaaacgct ggtcgaagtg atgaacgccg aaggcccgca gctgcacacc aacgccatcc
     6301 cgaaagcggt agtgaaaaat gccgatggta gcctgacgct ggagctggaa gatggtcgca
     6361 gtgaaacggt ggattgcctg atttgggcga ttggtcgcga gcctgccaat gacaacatca
     6421 acctagaagc cgctggcgtt aaaactaacg aaaaaggcta tatcgtcgtc gataaatatc
     6481 aaaacaccaa tattgaaggt atttacgcgg tgggcgataa cacgggtgca gtggaactga
     6541 caccggtagc agttgcagcg ggtcgccgtc tctctgaacg cctgtttaat aacaagccgg
     6601 atgagcatct ggattacagc aacattccga ccgtggtctt cagccatccg ccgattggta
     6661 ctgttggttt aacggaaccg caggcgcgcg agcagtacgg cgacgatcag gtgaaagtgt
     6721 ataaatcctc tttcaccgcg atgtataccg ccgtcaccac tcaccgccag ccgtgccgca
     6781 tgaagctggt gtgcgttggg ccagaagaga agattgtcgg tattcacggc attggctttg
     6841 gtatggacga aatgttgcag ggcttcgcgg tggcgctgaa gatgggcgca accaaaaaag
     6901 acttcgacaa taccgtcgcc attcacccaa cggcggcaga agagttcgtg accatgcgct
     6961 aaatgttaaa gggctaagag tagtgtgctc ttagccctta attacgtttc cgctatcagt
     7021 tcagaagctg aagcagaaag cggatcagtt ccagcagcgc aattaacgct ccaagaacga
     7081 tgattgcttt atcaatcacc cgttttctcc atgcgatgga gtgagaatgc atccgcttac
     7141 tcatccactg cctgtcacgg cgcatgtctc attgttagat gacgactatc tcactccggc
     7201 cagagcatca gttaacggca ccacccgtac ttctgaccag gactttgaaa gcgtttatgc
     7261 gcattaccag agtgaaaatg cctcagagct aactggataa tcatacagta cattcaggtt
     7321 ataaaaccag catgtccttg caatagtttc agtatggtat tagcattgat gcgttagatg
     7381 atgactatct cactccagcc agagccacca actcagggct gaagagtaaa aaaaccgacg
     7441 caaagtcggt ttttttacat ccggattcgg acaaggctta atatgacgat gacccagtga
     7501 aagtatataa atcgtcattg cgatatatac cgaagtgccc cctccgccag ctgaagaaat
     7561 cgctaattct tgcaatgtta gccactggct aatagtattg aaccgttaga tgtgggcatt
     7621 ctcactccag ccagagccac caactcaggc tgaagagtaa aaaaccgacg caaagtcggt
     7681 ttttttacgt cctgattcaa accgcctttc aaatgaatag ccaactcaaa attcacacct
     7741 attaccttcc tctgcactta cacattcgtt aagtcatata tgtttttgac ttatccgctt
     7801 cgaagagaga cactacctgc cacaatcagg agcgcaatat gtcatttctg ttacccatcc
     7861 aattgttcaa aattcttgct gatgaaaccc gtctgggcat cgttttactg ctcagcgagc
     7921 tgggagagtt atgcgtctgc gatctttgta ctgccctcga tcagtcgcag cccaagatct
     7981 cccgccacct ggcattgctt cgtgaaagcg ggctattgct ggaccgcaag caaggtaagt
     8041 gggttcatta ccgcttatca ccgcatattc cagcatgggc ggcgaaaatt attgatgagg
     8101 cctggcgatg tgaacaggaa aaggttcagg cgattgtccg caacctggct cgacaaaact
     8161 gttccgggga cagtaagaac atttgcagtt aaaaatttag ctaaacacat atgaattttc
     8221 agatgtgttt tatccgggag gcattatgtt actggcaggc gctatctttg tcctgaccat
     8281 cgtattggtt atctggcagc cgaaaggttt aggcatcggc tggagtgcaa cgctcggcgc
     8341 agtactggcg ttagttacgg gcgtggttca tccgggtgat attccggtgg tgtggaatat
     8401 cgtctggaac gcgacggctg cgtttatcgc cgtcattatc atcagcctgc tgctggatga
     8461 gtccggcttt tttgaatggg cggcgctgca cgtctcacgc tggggtaatg gtcgtggtcg
     8521 cttgctgttt acctggattg tcctgctcgg tgctgccgtt gccgccctgt ttgccaatga
     8581 tggcgcggcg cttattttga cgccgattgt catcgccatg ctgctggctt taggattcag
     8641 taaaggcacg acgctggcgt tcgtgatggc ggccggattc attgccgata ccgccagcct
     8701 gccgcttatt gtctccaacc tggtgaatat cgtttccgct gatttctttg gcctcggctt
     8761 tcgcgaatac gcctcggtga tggtgccggt ggatatcgcc gcgattgttg ccacgctggt
     8821 gatgctacat ctctattttc gcaaagatat tccgcagaac tacgatatgg cgctgctgaa
     8881 atctcccgca gaagcgatca aagatcctgc tacgttcaaa actggctggg ttgttttact
     8941 gcttctgctg gtgggatttt tcgtcctgga accgctcggc attccggtga gcgccattgc
     9001 agctgtgggc gcgctgatat tatttgtcgt cgctaaacgc ggtcatgcga ttaatacggg
     9061 taaagtcctg cgcggtgccc cctggcagat tgtcatcttc tcgctcggca tgtatctggt
     9121 ggtttatggc ctgcgcaatg ccggattaac ggaatatctt tctggcgtac tcaacgtgct
     9181 ggcggataac ggcctgtggg ccgcgacgct cggcaccgga ttcctcaccg ccttcctctc
     9241 ttctattatg aacaatatgc cgacggtact ggttggcgcg ttgtccattg atggcagcac
     9301 ggcatctggc gttatcaaag aagcgatggt ttatgccaat gtgattggct gcgatttggg
     9361 accgaaaatt accccaattg gtagcctggc tacgctactc tggctgcacg tactttcgca
     9421 gaagaatatg actatcagct ggggatatta cttccgtaca gggattatca tgaccctgcc
     9481 tgtgctgttt gtgacgctgg ctgcgctggc gctacgtctc tctttcactt tgtaatgaga
     9541 tactgatatg agcaacatta ccatttatca caacccggcc tgcggcacgt cgcgtaatac
     9601 gctggagatg atccgcaaca gcggcacaga accgaccatt atccattatc tggaaactcc
     9661 gccaacgcgc gatgaactgg tcaaactcat tgccgatatg gggatttccg tacgcgcgct
     9721 gctgcgtaaa aacgtcgaac cgtatgagga gctgggcctt gcggaagata aatttactga
     9781 cgatcggtta atcgacttta tgcttcagca cccgattctg attaatcgcc cgattgtggt
     9841 gacgccgctg ggaactcgcc tgtgccgccc ttcagaagtg gtgctggaaa ttctgccaga
     9901 tgcgcaaaaa ggcgcattct ccaaggaaga tggcgagaaa gtggttgatg aagcgggtaa
     9961 gcgcctgaaa taaagcggcg atatccactg ccgatagttg gtaaagtgcg ctttgtttat
    10021 gtcggatgcg acgctaacgc gtcttatccg acctacaaaa ccgtgcaaat tcaatatatg
    10081 gcatgaaata tgtagagcgt agcgcatcag gcaattttgc tatttacgca gcactcctcg
    10141 cgtaagaatt caattatcgc ttccagcatt gcgtattgcg aaacacacat cagcgtccga
    10201 ctctcacggt tttgtttgac cagtcctacc gataccagtg ctgatatatg gtggctgagt
    10261 gtggagccag gtatcccaag ctgcttttgc aactcaccga ccggcaaccc ttgttccccg
    10321 gctttgacaa gatgtttaaa tatgaacaaa cgggtcgggt gacctagctc tttcagcgct
    10381 ttagcgactt cttccaactg catatgacac ccctctgtct g
//
LOCUS       DAZOYL010000132        10420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZOYL010000132 DAZOYL010000000
VERSION     DAZOYL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.132"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..698)
                     /locus_tag="ACHUOY_002430"
     CDS             complement(<1..698)
                     /locus_tag="ACHUOY_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0683189.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            954..1109
                     /gene="rseD"
                     /locus_tag="ACHUOY_002431"
     CDS             954..1109
                     /gene="rseD"
                     /locus_tag="ACHUOY_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR0683190.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1106..1681
                     /gene="rpoE"
                     /locus_tag="ACHUOY_002432"
     CDS             1106..1681
                     /gene="rpoE"
                     /locus_tag="ACHUOY_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR0683191.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1714..2364
                     /gene="rseA"
                     /locus_tag="ACHUOY_002433"
     CDS             1714..2364
                     /gene="rseA"
                     /locus_tag="ACHUOY_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR0683192.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2364..3320
                     /gene="rseB"
                     /locus_tag="ACHUOY_002434"
     CDS             2364..3320
                     /gene="rseB"
                     /locus_tag="ACHUOY_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR0683193.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3317..3796
                     /gene="rseC"
                     /locus_tag="ACHUOY_002435"
     CDS             3317..3796
                     /gene="rseC"
                     /locus_tag="ACHUOY_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR0683194.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3994..5793
                     /gene="lepA"
                     /locus_tag="ACHUOY_002436"
     CDS             3994..5793
                     /gene="lepA"
                     /locus_tag="ACHUOY_002436"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR0683195.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5809..6783
                     /gene="lepB"
                     /locus_tag="ACHUOY_002437"
     CDS             5809..6783
                     /gene="lepB"
                     /locus_tag="ACHUOY_002437"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR0683196.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7056..7736
                     /gene="rnc"
                     /locus_tag="ACHUOY_002438"
     CDS             7056..7736
                     /gene="rnc"
                     /locus_tag="ACHUOY_002438"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR0683197.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7733..8638
                     /gene="era"
                     /locus_tag="ACHUOY_002439"
     CDS             7733..8638
                     /gene="era"
                     /locus_tag="ACHUOY_002439"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR0683198.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8650..9378
                     /gene="recO"
                     /locus_tag="ACHUOY_002440"
     CDS             8650..9378
                     /gene="recO"
                     /locus_tag="ACHUOY_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR0683199.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9390..10121
                     /gene="pdxJ"
                     /locus_tag="ACHUOY_002441"
     CDS             9390..10121
                     /gene="pdxJ"
                     /locus_tag="ACHUOY_002441"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR0683200.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10121..>10420
                     /gene="acpS"
                     /locus_tag="ACHUOY_002442"
     CDS             10121..>10420
                     /gene="acpS"
                     /locus_tag="ACHUOY_002442"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR0683201.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAE"
BASE COUNT         2589 a         2579 c         2807 g         2445 t
ORIGIN      
        1 cagcccgcgc gccacgccat agcaatgcca tcgccagaag aaatatccgg attggtggtg
       61 tactgataca ccttcgacgc accgccggtt gccagtacca ctgcttttgc gtggcaggtt
      121 tccaccgttt ctttatttcg gttccatacc cacgcgccta caacccgtcg cgtgcccggc
      181 aggccaattt tgtcagagac aatcagatca actgcgttgc tgcgctccag cacacgaata
      241 ttcggatggt tctgcgcctt gctcaccagc gtggattgta cttctctacc ggtggcgtcg
      301 gcggcatgaa gaatacgacg gtgactatgt ccaccttcac gggtcagatg gtagctttct
      361 tcgccattcg gttgaacgtg ggtatcaaac aaaaccccct ggtcaattaa ccattgcaca
      421 caggaacgag cattgctggc gacaaattca actgcatggc gatcgcaaat accagccccg
      481 gcaatcaatg tgtcttccac atgcgagtca atgctgtcag tttcatcaaa cacggcggca
      541 ataccgccct gggcataaaa tgttgaacct tccgttaccg ggcctttact tagaacgatg
      601 acctgatgct ggtcagccag gcgtagcgcc agtgaaagtc cggctgcgcc gctaccaata
      661 atcaacacgt cacatgaatg ttcagggaga gtattcattt tctttgttta atttactaaa
      721 cattgtttgg tcagcatagc atcatgttgt gcgaataaac acctgctatt ttaatatttg
      781 ttacagtggc taaacacgtt gacgcagggc ggcgagaaaa agagaagtta ctggctggtg
      841 gaggattagg tggtgaaata aaaaggccgt tgggttactc ttcaggcagt taaatgggca
      901 tttctacaca gataatgcga tgttcagatt ctgtagactt ataatgatag ataatgatcc
      961 gtctacagca tgacaaacaa aaacagatgc gttacggaac tttacaaaaa cgagacactc
     1021 taaccctttg cttgctcaaa ttgcagctaa tggagtggcg tttcgatagc gcgtggaaat
     1081 ttggtttggg gagactttac ctcggatgag cgagcagtta acggaccagg tcctggttga
     1141 acgggtccag aagggagatc agaaagcctt taacttactg gtagtgcgct atcagcataa
     1201 agtggcgagt ctggtttccc gctatgtgcc gtcgggtgat gttcccgatg tggtacaaga
     1261 agcttttatt aaagcctatc gtgcgctgga ttcgttccgg ggagatagcg ctttttatac
     1321 atggctgtat cggattgctg taaatacagc gaaaaattac ctggttgctc aggggcgtcg
     1381 tccaccttcc agtgatgtgg atgccattga agctgaaaac ttcgaaagtg gcggcgcgtt
     1441 gaaagaaatt tcgaaccctg agaacttaat gttgtcagaa gaactgagac agatagtttt
     1501 ccgaactatt gagtccctcc cggaagattt gcgcatggca ataaccttgc gggagctgga
     1561 tggcctgagc tatgaagaga tagccgctat catggattgt ccggtaggta cggtgcgttc
     1621 acgtatcttc cgagcgaggg aagctattga taacaaagtt caaccgctta tcaggcgttg
     1681 acgatagcgg gatactggat aagggtatta ggcatgcaga aagaacaact ttccgcttta
     1741 atggatggcg aaacgctgga tagtgagctg cttaacgaac tggctcataa cccagaaatg
     1801 cagaaaacct gggaaagcta tcacttaatc cgtgattcaa tgcggggtga tactcccgag
     1861 gtgctccatt tcgatatctc ttcacgcgtg atggccgcca ttgaagaaga gccagtacgt
     1921 caaccggcga cattgatccc ggaagcccag cctgcgccgc atcaatggca caaaatgcca
     1981 ttctggcaga aagtacgtcc gtgggcggca cagcttaccc aaatgggcgt agcagcatgc
     2041 gtatcgcttg cagttatcgt tggcgtccag cactataatg gacaatctga aacgtcccag
     2101 cagcccgaaa cgccggtatt taatacactg ccgatgatgg gtaaagccag cccggtaagc
     2161 ctgggagtac cttctgaagc gaccgcaaac aatggtcaac agcagcaggt acaggagcag
     2221 cgtcgtcgca ttaatgcaat gttgcaggat tacgaactgc aacgccgact ccactctgaa
     2281 cagcttcagt ttgagcaggc gcaaacccag caagccgctg tacaggtgcc aggacttcaa
     2341 actttaggaa cgcaatcgca gtaatgaagc aactttggtt tgccatgtca ttagtgacag
     2401 gtagcctgtt attctctgct aacgcctcgg ccactcccgc gtccggggcg ttattacagc
     2461 agatgaacct ggccagtcag tcactgaatt acgagctgtc attcatcagc atcaataaac
     2521 agggtgttga gtctctgcgt tatcgacatg cacgcctcga taaccgtcct cttgcacaat
     2581 tgttgcaaat ggatggcccg cgccgggaag tggtacagcg cggcaatgaa atcagctatt
     2641 ttgaaccggg acttgaaccg ttcacgctta atggcgatta cattgttgat tctctgccat
     2701 cgcttatcta taccgatttc aaacgccttt ctccttacta cgactttatc tccgtcgggc
     2761 gcacgcgtat tgctgatcgt ctttgcgaag tcattcgcgt ggttgcccga gatggtacac
     2821 gctacagcta catcgtgtgg atggacactg aatcgaaatt accgatgagg gttgatctcc
     2881 ttgatcgcga tggtgaaacg ctggaacaat ttcgcgtgat ttcttttaac gtcaatcagg
     2941 atatcagcag cagtatgcag acgctggcga aggcaaattt gccgccgttg ctttctgttc
     3001 ctgtaggtga aaaagctaaa ttcagctgga cgccaacctg gttgccacag ggttttagcg
     3061 aagtttccag tagtcgacgt ccgctaccga cgatggacaa catgcctatc gaatcacgtc
     3121 tctattccga cggattattc agcttctcgg taaacgttaa ccgcgctacg ccatcgagca
     3181 ccgatcagat gttgcgcacc ggacgcagaa ccgtcagtac aagcgtacgt gataacgccg
     3241 aaatcaccat tgtcggtgaa ctgccgccgc aaacggcgaa acgcattgcc gagaatatta
     3301 agttcggggc agcgcaatga tcaaagagtg ggctaccgtc gtctcctggc aaaacgggca
     3361 ggcgctggtc agttgtgatg ttaaagcctc atgcagcagc tgcgcttcac gcgccggttg
     3421 cggtagccgc gtgttgaata aacttggccc gcaaaccacg cataccattg tcgtaccctg
     3481 tgatgaaccg ttagtgccgg ggcaaaaagt ggaattgggg atcgccgaag gcagcctgct
     3541 tagctccgca ttactggttt atatgtcgcc gctggtggga ttattcctta tcgcttcgct
     3601 atttcagcta ctctttgctt ctgacgttgc agcattatgt ggtgcgatcc ttggcggtat
     3661 tggcgggttt ctgattgcgc gcggttactc gcgtaagttt gctgcccggg cagaatggca
     3721 accgatcata ttaagcgtgg cattgccgcc tgaccttgtg cgatttgaaa catcttcgga
     3781 agacgcgagc cagtgatttc actggctttg ttgcattttg cataacaaat gcgaggacgt
     3841 ttccagatac cacaatccat acggtatgaa catttcctcg cctcaatgtt gtagtgtaga
     3901 atgcggcgtt tctattaata cagacgttaa gctcagaaca gcgacttcta aaagcctggt
     3961 taaccgggca ttaaggcaca ataatcatac tttatgaaga atatacgtaa cttttcgatc
     4021 atagctcaca ttgaccacgg taaatcgacg ctgtctgacc gtattatcca gatctgcggt
     4081 ggcctgtctg accgtgaaat ggaggcgcag gttctcgatt ccatggatct tgagcgtgag
     4141 cgtggcatta ccatcaaagc gcaaagcgtg acgctggact acaaagcgtc tgacggcgaa
     4201 acctatcagc ttaactttat cgacaccccg ggccacgtag acttctccta tgaagtttcc
     4261 cgttcgctgg ctgcctgtga aggtgcattg ctggtggtcg acgccgggca gggcgtggaa
     4321 gcgcaaaccc tggcaaactg ctacaccgcc atggaaatgg atctcgaagt ggtgccagta
     4381 ctgaacaaga ttgacctgcc ggcagccgat cctgaacgcg tggcggaaga aattgaagat
     4441 atcgtcggca tcgacgccac tgacgcggtg cgctgttcag cgaaaaccgg cgttggcgtg
     4501 caggacgttc tcgaacgtct ggtgcgcgac attccgccgc cggaaggcga tccggaaggc
     4561 tcgttgcagg cactaattat cgactcatgg ttcgacaact acctgggcgt tgtttcactt
     4621 atccgtatta aaaacggcac cctgcgtaag ggcgacaaag tgaaagtcat gagtaccggg
     4681 cagacctata acgccgaccg tctgggcatc ttcacgccga aacaggttga ccgcactgaa
     4741 ctgaaatgtg gcgaagtagg ctggctggta tgtgcgatta aagatatcca tggcgctcca
     4801 gtcggcgata ccttaacgct ggcgcgtaat ccggcagaaa aggcgctgcc tggctttaag
     4861 aaagtcaaac cgcaggtata cgccggtctg ttcccggtaa gttccgacga ctatgaagcc
     4921 ttccgtgacg cgctgggtaa actcagcctg aatgatgcct cactgttcta tgagccggaa
     4981 agctccagcg cgctgggctt tggtttccgc tgcggcttcc ttggcctgct gcacatggag
     5041 atcatccagg aacgtctgga acgtgaatac gatctggatc tgatcaccac tgcgccgacc
     5101 gtagtgtatg aagttgaaac cacgtcaagg gaagttatct acgtcgacag cccatccaag
     5161 ctgcctgcgg taaataacat ttacgaactg cgcgagccga ttgcagagtg tcacatgctg
     5221 ctgccgcagg catatctcgg caacgttatt acgctgtgcg tagaaaaacg cggcgtgcag
     5281 accaatatgg tttaccacgg taatcaggtg gcgctgacgt acgagatccc gatggcggaa
     5341 gtggtgctcg acttcttcga tcgcctgaaa tctacctcgc gtggttatgc gtctctggat
     5401 tacaacttca aacgcttcca ggcgtccgac atggtacgtg tagacgtatt aatcaacggt
     5461 gaacgtgttg atgcgttggc gctgatcacc caccgtgata attcgcaaaa ccgcggtcgc
     5521 gagttggtgg agaagatgaa agatctgatc ccacgccagc agtttgatat cgccattcag
     5581 gcagcgattg gtacgcacat tattgcgcga tccaccgtga aacagctgcg taaaaacgta
     5641 ctggctaaat gttatggcgg cgatatcagc cgtaagaaaa agctgctgca gaagcagaaa
     5701 gaaggtaaga aacgcatgaa gcagatcggt aacgtcgagc tgccgcagga agcgttcctc
     5761 gccattctgc acgtcggcaa agacaacaaa taacccttag gagttggcat ggcgaatatg
     5821 tttgccctga ttctggtgat tgccacactg gtgacgggca ttttatggtg cgtggataaa
     5881 ttctttttcg cacctaaacg gcgggaacgt caggcagcgg cgcaggcggc tgccggtgac
     5941 tcactggata aagcaacgtt gaaaaaggtt gcaccgaagc ttggctggct ggaaaccgga
     6001 gcttctgttt ttccggtgct ggctatcgta ttgattgtac gttcgtttat ttatgaaccg
     6061 ttccagatcc cgtcaggttc gatgatgccg actctgttaa tcggtgattt tattctggta
     6121 gagaagtttg cttatggcat taaagatcct atctaccaga aaacgctgat cgaaaccggt
     6181 catccgaaac gcggcgatat cgtggtcttt aaatatccgg aagatccaaa gcttgattac
     6241 atcaagcgcg cggtgggttt accgggcgat aaagtcactt acgatccggt ctcaaaagag
     6301 ctgacgattc aaccgggatg cagttccggc caggcgtgtg aaaacgcgct gccggtcacc
     6361 tactcaaacg tggaaccgag cgatttcgtt cagaccttct cacgccgtaa tggtggggaa
     6421 gcgaccagcg gattctttga agtgccgaaa aacgaaacca aagaaaatgg aattcgtctt
     6481 tccgagcgta aagagacact gggtgatgtg acgcaccgaa ttctgacagt gccgattgcg
     6541 caggaccagg tggggatgta ttaccagcag ccagggcaac aactggcaac ctggattgtt
     6601 ccgccgggac aatacttcat gatgggcgac aaccgcgaca acagcgcgga cagccgttac
     6661 tggggctttg tgcctgaagc gaatctggtc ggtcgggcaa cggctatctg gatgagtttc
     6721 gataagcaag aaggcgaatg gccgacaggt gtgcgcttaa gtcgcattgg cggcatccat
     6781 taatagccca tcttcgttca cgttgtcgcc gttatggcga caacgtgaat tatttatgag
     6841 ataaatctcc cgtggctaac gacatccccc gtcgttgtgt atagaatatt cccccgaagt
     6901 ttaaggttgg cacctccagg ttgccacggc acacgaaaca gcgttggtcc ccatataccg
     6961 gtaaactgaa actgcagcga agcagttagc agagccatgt atatcaggtc tgtttcgtgt
     7021 gctgaattgt tgacgcattt atttattggt atcgcatgaa ccccatcgta attaatcggc
     7081 ttcaacggaa gctgggctac acttttaatc atcaggaact gttgcagcag gcattaactc
     7141 atcgtagtgc cagcagcaaa cataacgagc gtttagaatt tttaggcgac tctattctga
     7201 gctacgttat cgccaatgcg ctttatcacc gtttccctcg tgtggatgaa ggcgatatga
     7261 gccggatgcg cgccacgctg gtccgtggca atacgctggc ggaactggcg cgcgaatttg
     7321 agttaggcga gtgcttacgt ttagggccag gtgaacttaa aagcggtgga tttcgtcgtg
     7381 agtcaattct cgccgatacc gtcgaagcat taattggtgg cgtattcctc gacagtgata
     7441 ttcaaaccgt cgagaaatta atcctcaact ggtatcaaac tcgtttggac gaaattagcc
     7501 caggcgataa acaaaaagat ccgaaaacgc gcttgcaaga atatttgcag ggtcgccatc
     7561 tgccgctgcc gacttatctg gtagtccagg tacgtggcga agcgcacgat caggaattta
     7621 ctatccactg ccaggtcagc ggcctgagtg aaccggtggt tggcacaggt tcaagccgtc
     7681 gtaaggctga gcaggctgcc gccgaacagg cgttgaaaaa actggagctg gaatgagcat
     7741 cgataaaagt tactgcggat ttattgccat cgtcggacgt ccgaacgttg gcaaatccac
     7801 attgttgaac aaactgctgg ggcagaagat ctccatcacc tcccgcaaag cgcaaacaac
     7861 tcgtcaccgc attgtgggga tccatactga aggcgcgtat caggcgatct acgttgatac
     7921 ccctggcctg catatggaag aaaaacgcgc tattaaccgc ctgatgaaca aagcggcgag
     7981 cagctctatt ggcgacgttg agctggtgat ttttgtcgtt gaaggcaccc gctggacgcc
     8041 ggacgacgaa atggtgctca acaaactgcg cgatggcaaa gcgccggtga tcctcgcggt
     8101 gaacaaagtg gataacgtgc aggaaaaagc cgatctgctg ccgcacttac agttcctggc
     8161 aagtcagatg aacttcctcg atatcgtgcc gatctctgct gaaaccgggc tgaatgtcga
     8221 cactatcgca gcaatcgtgc gtaagcatct gcctgaagcg acccatcact tcccggaaga
     8281 ttacatcacc gatcgctcac agcgttttat ggcgtctgaa atcatccgcg aaaaactgat
     8341 gcgtttcctc ggcgctgaac tgccgtactc cgtgaccgtg gagatcgaac gtttcgtctc
     8401 taacgaacgc ggtggttatg acatcaatgg tttgattctc gttgagcgtg aagggcagaa
     8461 gaagatggtt attggcaaca aaggggccaa gatcaaaacc atcgggattg aagcgcgtaa
     8521 agacatgcag gaaatgttcg aagcgcctgt ccaccttgaa ctgtgggtaa aagtgaaatc
     8581 cggttgggcc gacgacgaac gcgcactgcg cagtctcggt tacgttgacg atctttaaga
     8641 gtcactccga tggaaggctg gcagcgcgca tttgtcctgc atagtcgccc gtggagcgaa
     8701 accagcctga tgctggacgt cttcacggag gaatcgggtc gcgtgcgtct ggttgccaaa
     8761 ggcgcacgct ctaaacgctc tactctgaaa ggtgcattac agcctttcac ccctctcttg
     8821 ctacgtttcg gcgggcgtgg cgaagtcaaa acgctgcgca gtgctgaagc tgtctcgctg
     8881 gcgctgccat taagcggtat cacgctttac agcggtctgt acatcaacga acttctctcc
     8941 cgcgtactgg aatacgagac gcgcttctct gaactctttt tcgattactt gcactgcatt
     9001 cagtctcttg caggtgccac tggtacgcca gaacccgcgc tgcgccgctt tgaactggca
     9061 ctgctcgggc atctgggcta tggcgtcaat tttacccatt gcgcgggtag cggcgagccg
     9121 gtagatgaca ccatgacgta tcgttatcgc gaagaaaaag ggtttatcgc aagcgtcgtt
     9181 atcgacaata aaacgttcac cggaaggcag ttaaaagcgt taaacgcgcg ggaatttcct
     9241 gacgcagaca cactacgcgc cgcgaaacgc tttacccgca tggcgcttaa gccgtatctt
     9301 ggcggtaaac cattaaagag cagggaactg ttccggcagt ttatgcctaa gcgaacggtg
     9361 aaaacacatt atgaatgatg aggattgtca tggctgaatt actgttaggc gtcaacattg
     9421 accatattgc aacgctgcgt aacgcacgcg gtactgctta cccggatccg gtgcaggcgg
     9481 cgtttattgc cgagcaggcg ggagcggacg gcattaccgt gcatttacgt gaagatcgcc
     9541 gccacattac tgaccgcgat gtgcgcatcc tgcggcagac gctggatacc cgaatgaatc
     9601 tggagatggc agtgaccgaa gagatgctgg cgatcgccgt tgagacgaag ccacattttt
     9661 gctgcctggt accggaaaag cgtcaggaag taacaaccga aggcggcctg gacgttgcag
     9721 ggcagcgtga aaaaatgcgc gatgcctgca aacgtctgac agatgccggg attcagattt
     9781 ctctctttat cgacgccgat gaagagcaga tcaaagctgc ggcagaggtt ggcgcaccgt
     9841 ttatcgagat ccacaccggt tgctatgctg atgccaaaac tgacgccgaa caggcgcaag
     9901 agctggtgcg tatcgccaaa gccgcgacct ttgccgctag tctcggtctg aaagttaacg
     9961 ccggacacgg tctgacctat cacaacgtga aagccattgc cgccatccct gagatgcatg
    10021 aactgaatat cggtcatgcc attattggcc gtgcagtgat gaccggactg aaagatgcgg
    10081 tggcagaaat gaagcgtctg atgctggaag cgcgtggcta atggcaatat taggtttagg
    10141 cacggatatc gtagagatcg ctcgcataga agcagtgatc gcccgatccg gtgagcgcct
    10201 ggcacgccgc gtattaagcg ataacgaatg ggcaatctgg aaaacgcacc accagccggt
    10261 gcgttttctg gcgaagcgtt ttgctgtgaa agaagccgca gcaaaagcgt ttggcaccgg
    10321 gatccgcaat ggtctggcgt ttaatcaatt tgaagtattc aatgatgagc tcggcaaacc
    10381 acggctacgg ctatggggtg aggcattaaa actggcggaa
//
LOCUS       DAZOYL010000133        10368 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZOYL010000133 DAZOYL010000000
VERSION     DAZOYL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.133"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHUOY_002443"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHUOY_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR0683202.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUOY_002444"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUOY_002444"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR0683203.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUOY_002445"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUOY_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR0683204.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUOY_002446"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUOY_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR0683205.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUOY_002447"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUOY_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUOY_002448"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUOY_002448"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0683206.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVTAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUOY_002449"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUOY_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR0683207.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUOY_002450"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUOY_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR0683208.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHUOY_002451"
     CDS             complement(9457..10149)
                     /locus_tag="ACHUOY_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR0683209.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2530 a         2504 c         2738 g         2596 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tgactgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgcc
//
LOCUS       DAZOYL010000134        10325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZOYL010000134 DAZOYL010000000
VERSION     DAZOYL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.134"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            111..1505
                     /gene="galP"
                     /locus_tag="ACHUOY_002452"
     CDS             111..1505
                     /gene="galP"
                     /locus_tag="ACHUOY_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR0683210.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1582..2079
                     /locus_tag="ACHUOY_002453"
     CDS             1582..2079
                     /locus_tag="ACHUOY_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR0683211.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2174..2881
                     /gene="endA"
                     /locus_tag="ACHUOY_002454"
     CDS             2174..2881
                     /gene="endA"
                     /locus_tag="ACHUOY_002454"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR0683212.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2961..3692
                     /gene="rsmE"
                     /locus_tag="ACHUOY_002455"
     CDS             2961..3692
                     /gene="rsmE"
                     /locus_tag="ACHUOY_002455"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR0683213.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3705..4655
                     /gene="gshB"
                     /locus_tag="ACHUOY_002456"
     CDS             3705..4655
                     /gene="gshB"
                     /locus_tag="ACHUOY_002456"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR0683214.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4764..5327
                     /locus_tag="ACHUOY_002457"
     CDS             4764..5327
                     /locus_tag="ACHUOY_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR0683215.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5327..5743
                     /gene="ruvX"
                     /locus_tag="ACHUOY_002458"
     CDS             5327..5743
                     /gene="ruvX"
                     /locus_tag="ACHUOY_002458"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR0683216.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5918..6898)
                     /locus_tag="ACHUOY_002459"
     CDS             complement(5918..6898)
                     /locus_tag="ACHUOY_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR0683217.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            6916..7620
                     /gene="yggS"
                     /locus_tag="ACHUOY_002460"
     CDS             6916..7620
                     /gene="yggS"
                     /locus_tag="ACHUOY_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR0683218.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7638..8204
                     /gene="yggT"
                     /locus_tag="ACHUOY_002461"
     CDS             7638..8204
                     /gene="yggT"
                     /locus_tag="ACHUOY_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR0683219.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8201..8491
                     /gene="yggU"
                     /locus_tag="ACHUOY_002462"
     CDS             8201..8491
                     /gene="yggU"
                     /locus_tag="ACHUOY_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR0683220.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIVALIN"
     gene            8499..9092
                     /locus_tag="ACHUOY_002463"
     CDS             8499..9092
                     /locus_tag="ACHUOY_002463"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR0683221.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9085..10221
                     /gene="hemW"
                     /locus_tag="ACHUOY_002464"
     CDS             9085..10221
                     /gene="hemW"
                     /locus_tag="ACHUOY_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR0683222.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2418 a         2716 c         2739 g         2452 t
ORIGIN      
        1 agataaatgt tagtgtaagc gattacactg atgtgatttg cttcacatct ttttacgtcg
       61 tactcaccta tcttaattca caataaaaaa taaccatatt ggagggcatc atgcctgacg
      121 ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt cgtctgcttc cttgccgctc
      181 tggcgggatt actctttggc ctggatatcg gtgtaattgc tggcgcactg ccgtttattg
      241 cagatgaatt ccagattact tcgcacacgc aagaatgggt cgtaagctcc atgatgttcg
      301 gtgcggcagt cggtgcggtg ggcagcggct ggctctcctt taaactcggg cgcaaaaaga
      361 gcctgatgat cggcgcaatt ttgtttgttg ccggttcgct gttctctgcg gctgcgccaa
      421 acgttgaagt actgattctt tcccgcgttc tactggggct ggcggtgggt gtggcctctt
      481 ataccgcacc gctgtacctc tctgaaattg cgccggaaaa aattcgtggc agtatgatct
      541 cgatgtatca gttgatgatc actatcggga tcctcggtgc ttatctttct gataccgcct
      601 tcagctacac cggtgcatgg cgctggatgc tgggtgtgat tatcatcccg gcaattttgc
      661 tgctgattgg tgtcttcttc ctgccagaca gcccacgttg gtttgccgcc aaacgccgtt
      721 ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac cagcgcggaa gcgaaacgcg
      781 aactggatga aatccgtgaa agtttgcagg ttaaacagag tggctgggcg ctgtttaaag
      841 agaacagcaa cttccgccgc gcggtgttcc ttggcgtact gttgcaggta atgcagcaat
      901 tcaccgggat gaacgtcatc atgtattacg cgccgaaaat cttcgaactg gcgggttata
      961 ccaacaccac agagcaaatg tgggggacag tgattgtcgg cctgaccaac gtacttgcca
     1021 cctttatcgc aatcggcctt gttgaccgct ggggacgaaa accaacgcta acgctgggct
     1081 tcctggtgat ggctgctggc atgggcgtac tcggtacaat gatgcatatc ggtatccact
     1141 ctccgtcggc gcagtatttc gccatcgcca tgctgctgat gtttattgtc ggttttgcca
     1201 tgagtgccgg tccgctgatt tgggtactgt gctccgaaat tcagccgctg aaaggccgcg
     1261 attttggcat cacctgctcc actgccacca actggattgc caacatgatc gttggcgcaa
     1321 cgttcctgac catgctcaac acgctgggta acgccaacac cttctgggtg tatgcggctc
     1381 tgaacgtact gtttatcctg ctgacattgt ggctggtacc ggaaaccaaa cacgtttcgc
     1441 tggaacatat tgaacgtaac ctgatgaaag gtcgtaaact gcgcgaaatc ggcgctcacg
     1501 attaatctcc tcaggcttcc tcccgtcgcg gaggaagccg cctcttgcag tcatcttttc
     1561 ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc ctatcgccat ccaacaggcc
     1621 gttatgcgcg gcctgcggga aaaactcgcc caggccaacc tgaagctagg gcgtaactac
     1681 ccggagccaa aactctctta cacccagcgc ggaacctccg ccggaacagc ctggctggaa
     1741 agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa acagtgaagc ttttattgaa
     1801 gaagtggtac cgcacgaact ggcacatttg ctggtatgga aacatttcgg ccgcgtagcg
     1861 ccacatggca aagagtggaa gtggatgatg gaaagcgtgc tgggtgttcc cgcccgtcgt
     1921 acgcatcagt tcgaactgca atccgtgcgt cgcaacacct tcccctaccg ctgcaagtgc
     1981 caggagcatc agcttaccgt acgccgccat aatcgcgtag ttcgtggcga ggccgtctat
     2041 cgctgtgttc actgcggtga acagctggtt gcgaaataac catctgaact atcaggaact
     2101 ttcctgatct ggctgattgc ataccaaaac aactttcgct acgttgctgg ctcgttttaa
     2161 cacggagtaa gtgatgtacc gttatttgtc tattgctgcg gtggtactga gcgcagcatt
     2221 ttccggcccg gcgttggccg aaggtatcaa tagtttttct caggcgaaag ccgcggcggt
     2281 aaaagtccac gctgacgcgc ccggtacgtt ttattgcgga tgtaaaatta actggcaggg
     2341 caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag gtgcgcaaaa atgaaaaccg
     2401 agccagccgc gtagagtggg aacacgtcgt tcccgcctgg cagttcggtc accagcgcca
     2461 gtgctggcag gacggtggac gtaaaaactg cgctaaagat ccggtctatc gcaagatgga
     2521 aagcgatatg tataacctgc agccgtcagt cggtgaggtg aatggcgatc gcggcaactt
     2581 tatgtacagc cagtggaatg gcggcgaagg ccagtacggt caatgcgcca tgaaggtcga
     2641 tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc ggtgccattg cgcgcaccta
     2701 cttctatatg cgcgaccaat acaacctgac actctctcgc cagcaaacgc agctgttcaa
     2761 cgcatggaac aagatgtatc cggttaccga ctgggagtgc gagcgcgatg aacgcatcgc
     2821 gaaggtgcaa ggcaatcata acccgtatgt gcaacgcgct tgccaggcgc gaaagagcta
     2881 acctacacta gcgggattct ttttgttaac ccctacccca cgcgtacaac cgcgtgggga
     2941 gacgacgcgg atttttaact atgcgtatcc cccgcattta tcatcctgaa ccactgacca
     3001 gccattctca catcgcgctt tgcgaagatg ccgccaacca tatcgggcgc gtactgcgca
     3061 tggggccggg gcaggcgttg caattgtttg acggtagcaa ccaggtcttt gacgccgaaa
     3121 ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct ggaaggcaag atcgacgatc
     3181 gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc gcgtggtgaa aaaatggaat
     3241 ttacgatcca gaaatcgatc gaactcggtg taagcctcat tacgccactt ttttctgagc
     3301 gctgcggcgt taaactggat agtgaacgtc tgaacaagaa gctccagcag tggcagaaga
     3361 ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt gccggaaatc cgtccagcga
     3421 tggatctgga agcctggtgt gcagagcagg atgaaggact gaaactgaat cttcacccgc
     3481 gcgccagtaa cagcatcaat acgttgccgt taccggttga acgcgtccgc ctgctgattg
     3541 gcccggaagg cggtttatcg gcagatgaaa ttgccatgac tgcccgctat caatttactg
     3601 atatcctgtt gggacctcgc gttttgcgta cagagacaac tgcgctcacc gccattaccg
     3661 cgctacaagt acgatttggc gatttgggct aacggagaag aataatgatc aagctcggca
     3721 tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga ttccagtttc gctatgttgc
     3781 tggaagcaca gcgtcgtggt tacgaacttc actatatgga gatgggcgat ctgtatctga
     3841 tcaatggcga agcccgcgcc catacccgca cgctgaacgt gaagcagaac tacgaagagt
     3901 ggttttcgtt cgtcggtgaa caggatctgc cgctggccga tctcgatgtg atcctgatgc
     3961 gtaaagaccc gccgtttgat accgagttta tctacgcgac ctatattctg gaacgtgccg
     4021 aagagaaagg gacgctgatc gttaacaagc cgcagagcct gcgcgactgt aacgagaaac
     4081 tgtttaccgc ctggttctct gacttaacgc cagaaacgct ggttacgcgc aataaagcgc
     4141 agctaaaagc gttctgggag aaacacagcg acatcattct taagccgctg gacggtatgg
     4201 gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa cctcggcgtg attgccgaaa
     4261 ccctgactga gaatggcact cgctactgca tggcacaaaa ttacctgcca gccattaaag
     4321 atggcgacaa acgcgtgctg gtggtggatg gcgagccggt accgtactgc ctggcgcgta
     4381 ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg tggtcgcggt gaacctcgtc
     4441 cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg gccgacgctg aaagaaaaag
     4501 ggctgatttt tgttggtctg gatatcatcg gcgaccgtct gactgaaatt aacgtcacca
     4561 gcccaacctg tattcgtgag attgaagcag agtttccggt gtcgatcacc ggaatgttaa
     4621 tggatgccat cgaagcacgt ttacagcagc agtaacccac cttagcgaga aggatctcgt
     4681 tgagactctg agtgacagcg cccttctttc cacgcatact gggcgctgtt gcttttttga
     4741 accaggaaac agaacctctg acaatgaatt tacagcatca ctttcttatt gccatgcctg
     4801 ctctccagga tccgattttc cgtcgttccg tggtctacat ttgcgaacat aataccaatg
     4861 gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct caaaattgaa gggattctgg
     4921 aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat ccgtctggat aaaccggtta
     4981 tgctcggcgg tccgctggct gaagatcgcg ggtttatttt gcatactccg ccctccaatt
     5041 ttgcttccag cattcgcatt tcagacaaca cagtgatgac cacttcccgc gatgtgctgg
     5101 aaacgctcgg caccgataaa caaccgtctg acgtattggt ggctctaggt tatgcctcct
     5161 gggaaaaagg tcaactggaa caagaaattc tcgataacgc gtggctaacg gccccggcgg
     5221 atctgaatat tctgttcaaa acgccgattg ccgaccgctg gcgcgaggcg gcaaaactga
     5281 ttggtgtgga tattctcacc atgcctggtg tggcagggca cgcctgatga gtggaacctt
     5341 actcgccttc gacttcggca ccaaaagcat tggcgtagcg gtcggccaac gcattaccgg
     5401 cactgctcgc cctttgcctg caattaaagc acaggacggt acgccggact ggaacctcat
     5461 cgaacgttta ctgaaagagt ggcagccgga cgaaatcatc gtcggtttgc cgctgaatat
     5521 ggacggcacc gagcaaccat tgactgccag agcgcgtaaa tttgccaacc gtattcatgg
     5581 tcgtttcggt gttgaagtaa agctccatga cgaacgtctt agcaccgtgg aagcccgttc
     5641 cggtctgttt gaacagggcg gctatcgggc gctcaacaaa ggcaaagtcg actctgcctc
     5701 tgcggttatt attctcgaaa gctatttcga gcagggatat taaggcgatt taaaagcctg
     5761 gcggatagtc gttaccgcca tcttgccgga tgcggcgtga acgcctatcc ggcctacatg
     5821 tggcagcgat tgtaggtctg ataagacgcg caaacgtctc atcagacgtt tattgccttt
     5881 gtaggcccga taagcttgca catcgggcat ggcaacgtca caaacgccct tcccccactc
     5941 gctgctgaaa actctgctga aacgttatca tccccacctg ctgcccggtt tgaataacat
     6001 gcggtaactg gtgggttttc ccttcgcgaa tcaaattccc caccgcggga gtgttaatca
     6061 ataattcaaa cagcgccacg cgtccttcct gtttatccac ttccagcttt tgtgacaaca
     6121 cggcccgtaa actacctgcc agttgattac gtacgggatc tttttcctgc gccggaaatg
     6181 aatccaccag tcgctcaact gcctgcgcgg caccacgcgt atgtaatgtt gccagcacca
     6241 aatgcccggt ttctgccgcc gtcagtgcca gacggattgt ctcgctgtca cgcagctctc
     6301 cgagcaaaat cacatcagga tcttcccgca atgcggcccg caatcccgat gcgaacgtca
     6361 tacagtgcaa accaatttcc cgcttctgga tcaaacatcg ctggctggca tagatatatt
     6421 caacaggatc ttccagcgtc agaatatgcg catcggcatg ttgattgaga tagccaacca
     6481 tcgccgccag cgtggtagat ttgctgctcc ccgtcgcccc cgtcaccaga atcaggccat
     6541 tctcactctt gagtaattcc ggcaataccg ttggtgcgcc aagctgttcg agctgcgggc
     6601 agtgcgaagg taacagccgt aacgccagcg aaatgccatg ccgttgcgca aatgcgctgc
     6661 cacgcaatcg ctggttttcc gccagcgaca cggcaaaatc cagctggcca ttttccagca
     6721 atattgcccg ctgatcgtca tccagccact cccgcagtag ctcttcgacg tccggcgcct
     6781 caaacggcgc agcttccatt agcccgcgaa tgcgccatcg tgcgggccag gcgctgcaca
     6841 ggtgtagatc cgagacgtta tgctttacac taagggccac aatttcttcc atattcatac
     6901 taagatcctc ggaaaatgaa cgatattgcg cataacctgg cacaggtccg ggacaaaatc
     6961 tcagcggctg caacgcgttg cggccgttct ccagaagaaa ttacgctgct tgcagtcagt
     7021 aaaacaaaac ctgcgagcgc catcgcagaa gccattgatg ccgggcagcg tcaatttggt
     7081 gaaaactacg ttcaggaagg ggtagataaa attcgccact ttcaggaact gggcgtaaca
     7141 ggattagaat ggcattttat tggcccgttg cagtctaata aaagccgcct ggtggcagag
     7201 catttcgact ggtgtcatac catcgaccgt ttgcgcattg ctacccgcct caacgatcag
     7261 cgcccggcag aacttccccc tcttaacgta ctgattcaaa ttaatattag tgatgaaaac
     7321 agtaagtccg ggattcaact ggcagaactg gacgagctgg cagcggcggt cgctgaacta
     7381 ccgcgtttac gtctgcgcgg gctgatggca atccctgcgc ctgagtcaga atatgtaagg
     7441 cagtttgaag ttgcacgcca aatggctgta gcatttgccg gactgaaaac gcgctacccg
     7501 catatcgaca cgctctctct gggaatgtcg gacgatatgg aagccgccat tgcggcaggt
     7561 agcacgatgg ttcgtatcgg cactgcaatt tttggtgcgc gtgattactc taaaaaataa
     7621 ggaattaaag gaacgccatg aatacgttga ctttcctgct ttcaacggtc attgagctgt
     7681 ataccatggt gctgttatta cgcatctgga tgcagtgggc tcattgtgat ttttacaacc
     7741 ccttctcaca gtttgtagtg aaggtaacgc agccaattat cgggccactg cgccgcgtta
     7801 ttccggcaat gggccccatt gacagcgcct cgctgctggt tgcctatatt ctcagtttta
     7861 tcaaagccat cgtgctgttt aaagtggtga ccttcctgcc aatcatctgg attgctggtt
     7921 tactgattct gctgaaaacc atcggcctgc tgattttctg ggtcctgctg gtgatggcga
     7981 ttatgagctg ggtaagccag ggtcgtagtc cgattgaata cgtgctgatt cagctggccg
     8041 atccgctgct gcgcccgatt cgccgcctgc taccggcaat gggtgggatt gatttctcgc
     8101 cgatgatcct cgttctgctg ctgtatgtca tcaatatggg tgtcgcagaa gtattacagg
     8161 caaccggaaa catgctgctg ccggggctgt ggatggcgtt atgagtgccg taacagttaa
     8221 tgatgacggt ctggttttac ggctctatat tcagccgaaa gccagccgtg attctattgt
     8281 cggtttacat ggcgacgaag ttaaagtcgc cattaccgcg ccgccggttg acggccaggg
     8341 caacagtcat ctggtgaagt ttctcggtaa gcaattccgg gttgccaaaa gccaggtggt
     8401 gattgaaaaa ggcgaacttg gccgccacaa acaaattaaa atcattaatc cgcaacaaat
     8461 cccgccagaa atcgtggcgt taattaatta ggtatcctat gcaaaaagtt gtcctcgcaa
     8521 ccggcaatgc cggtaaagtg cgtgagctgg cgtcgctgct tagcgacttc ggtcttgata
     8581 tcgtggccca aacagacctc ggcgtagatt ccgctgaaga aaccggcctg acctttatcg
     8641 aaaacgcgat tctgaaagcg cgctatgcgg caaaagtgac cggtttaccg gcaattgccg
     8701 acgactctgg tctggcggta gatgcgctgg gtggcgcgcc gggtatttac tccgcgcgtt
     8761 attccggtga agacgcgacc gatcaaaaga atctgcaaaa actgttggaa acactgaaag
     8821 acgtaccgga cgaccaacgt caggcgcgtt tccactgcgt gctggtatat ctgcgtcacg
     8881 cggaagatcc gactccgctg gtatgccacg gcagctggcc gggcgtgatt actcgcgaac
     8941 cagcgggcac tggtggcttt ggttatgatc caatcttctt cgtaccttcc gaagggaaaa
     9001 ccgccgccga actgacccgc gaagaaaaga gcgccatttc ccaccgtggt caggcgttga
     9061 aactgctgct ggacgcttta cgtaatggtt aaattaccgc cgctgagtct ctacattcac
     9121 atcccgtggt gcgtgcagaa atgcccgtac tgcgatttca actctcacgc gttgaaagga
     9181 gaagtgccgc acgacgatta tgttcagcat ctgctttgcg atctggacaa tgatgtggct
     9241 tacgctcagg gccgtgaagt aaagacaatc tttattggcg gtggtacgcc gagcctgctt
     9301 tccggcccgg cgatgcaaac gctgctggac ggcgtgcgtg cgcgtttgcc gctggcagcg
     9361 gatgcagaaa ttactatgga agcgaaccct ggaacggtag aagccgatcg ctttgtcgat
     9421 tatcagcgtg ctggtgtgaa ccgcatctct attggtgtgc agagttttag cgaagaaaag
     9481 ctgaaacgac ttgggcgtat tcatggcccg caagaagcga aacgcgcggc aaagctggcg
     9541 agcgggctgg ggctgcgtag ctttaacctc gatttgatgc atggactacc ggatcaatca
     9601 ctggaagagg cgcttggcga tctacgccag gccattgaac tgaatccgcc gcatctttcc
     9661 tggtatcaac tgaccatcga acccaatacg ctgtttggtt cgcgaccacc ggtgctgccg
     9721 gacgatgacg cgctgtggga tattttcgaa caggggcatc agttattaac cgcagcgggt
     9781 tatcagcaat atgaaacttc cgcttacgcc aaacccggtt atcagtgcca gcacaatctc
     9841 aactactggc gatttggtga ctacatcggt attggctgcg gcgcgcacgg caaagtcacc
     9901 ttcccggatg ggcgcattct gcgtaccact aaaacgcgtc atcctcgtgg ttttatgcaa
     9961 ggaaggtatc tggaaagcca gcgtgatgtc gaagccgcag ataagccgtt tgagttcttt
    10021 atgaatcgct tccgtctgct ggaggccgcg ccgcgcgtgg agtttattgc gtataccggg
    10081 ctttgcgaag atgtgattcg cccacagtta gacgaggcga ttgcccaggg ttatctcacc
    10141 gaatgtgcgg attactggca gataaccgag cacggtaaat tattcttaaa ctcacttctt
    10201 gaattattcc tcgctgagta atcattaatg cgcctgcggg cgcattaatc acttctttcc
    10261 cccccgttaa ttaacttcgg caaaatatat ttcttactga ataaacatcg atgatattta
    10321 ttaga
//
LOCUS       DAZOYL010000135         9959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZOYL010000135 DAZOYL010000000
VERSION     DAZOYL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.135"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="ACHUOY_002465"
     CDS             complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="ACHUOY_002465"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR0683223.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="ACHUOY_002466"
     CDS             complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="ACHUOY_002466"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR0683224.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="ACHUOY_002467"
     CDS             complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="ACHUOY_002467"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0683225.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4042..4800
                     /gene="loiP"
                     /locus_tag="ACHUOY_002468"
                     /gene_synonym="yggG"
     CDS             4042..4800
                     /gene="loiP"
                     /locus_tag="ACHUOY_002468"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR0683226.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4943..5863)
                     /gene="speB"
                     /locus_tag="ACHUOY_002469"
     CDS             complement(4943..5863)
                     /gene="speB"
                     /locus_tag="ACHUOY_002469"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR0683227.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6001..7977)
                     /gene="speA"
                     /locus_tag="ACHUOY_002470"
     CDS             complement(6001..7977)
                     /gene="speA"
                     /locus_tag="ACHUOY_002470"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR0683228.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="ACHUOY_002471"
     CDS             complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="ACHUOY_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR0683229.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8211..8354)
                     /gene="yqgG"
                     /locus_tag="ACHUOY_002472"
     CDS             complement(8211..8354)
                     /gene="yqgG"
                     /locus_tag="ACHUOY_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR0683230.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8465..8716)
                     /gene="yqgD"
                     /locus_tag="ACHUOY_002473"
     CDS             complement(8465..8716)
                     /gene="yqgD"
                     /locus_tag="ACHUOY_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR0683231.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8772..9926
                     /gene="metK"
                     /locus_tag="ACHUOY_002474"
     CDS             8772..9926
                     /gene="metK"
                     /locus_tag="ACHUOY_002474"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR0683232.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2503 a         2646 c         2518 g         2292 t
ORIGIN      
        1 tagcaaagga caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt
       61 tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac
      121 cgtccagtaa gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg
      181 tcagcggctt catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa
      241 taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca
      301 gcagcagccc taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt
      361 taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga
      421 ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta
      481 ccagcgcctc aatacactct ttgacgagag tattggcaat taacaccgcc gggccgataa
      541 cttcaaaacc cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa
      601 ccatctcaaa accggcaggt atgcgttttt ccagtgcctt atcaacatat tttatgacca
      661 gcccaccgag cggccccata atcattgagc caaggaacat cgggatctct gcgccgacga
      721 tcacaccaat agtacctatt ccgcccatga ccgcgccgcg tttaccgccg accagatgac
      781 caccggtaga accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg
      841 cgaaatgttc gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg
      901 caataaaagc accaatattg gggatgacca ttgccgtcaa aaagccccca aaagcctgga
      961 cctttgcacg agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc
     1021 cgcggctgat tatgtccgct aattgttttt ctgatgctgc tgttaagagt tgttcgagta
     1081 tttcttcttc gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat
     1141 cggcagcaga aaggccgatg agtaatttta ttggttcgtc attccccgga aaataaacac
     1201 cttgttcaag taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat
     1261 gaggcattgc cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt
     1321 taattgcctg aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga
     1381 aatcgatagc ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa
     1441 aatagtcact aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta
     1501 aaaataaaat actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg gtgttttgaa
     1561 aacagtgaat tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa
     1621 tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc
     1681 tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt
     1741 cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg
     1801 acgttgtcga cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg
     1861 aaggtggtca taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca
     1921 atacccgctt ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa
     1981 gccgcatcct gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg
     2041 ccttcggcag tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg
     2101 atgaagatca gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg
     2161 tttgccagtt gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc
     2221 gcggtcggac cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg
     2281 tcacacggac gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc
     2341 ggctggtgag tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc
     2401 tgacgctgtt tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc
     2461 aggaaggtgg aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg
     2521 gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt
     2581 gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg
     2641 aattccggca acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg
     2701 attttcgccg gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca
     2761 gacggcattt cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc
     2821 gcgtaagccg caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca
     2881 tcccactgcg catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt
     2941 tcgcgggtca gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg
     3001 gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca
     3061 gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg
     3121 tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag
     3181 ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc
     3241 agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg
     3301 tcgcccatga aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc
     3361 gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc
     3421 ggaccggtgg tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta
     3481 gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc
     3541 agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca
     3601 gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg
     3661 tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg
     3721 ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg
     3781 atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa
     3841 tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc
     3901 agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt
     3961 ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg
     4021 accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca
     4081 acggtactga ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa
     4141 gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt
     4201 caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg
     4261 acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg
     4321 tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc
     4381 gggctgatgg atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg
     4441 cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg
     4501 cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt
     4561 gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat
     4621 gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc
     4681 tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg
     4741 gcatccgccg aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa
     4801 agcctggtgg tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg
     4861 cacatttgtt accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta
     4921 cgggtggcat cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct
     4981 ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca
     5041 cttccactac gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag
     5101 cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat
     5161 ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt
     5221 gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc
     5281 cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca
     5341 gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat
     5401 aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag
     5461 cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg
     5521 cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa
     5581 aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc
     5641 acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac
     5701 cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc
     5761 agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat
     5821 tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac
     5881 aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg
     5941 tcacaataaa ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac
     6001 ttactcatct tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg
     6061 ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag
     6121 cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga
     6181 cagttctact tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc
     6241 gaacaggttg tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag
     6301 catcggcgga ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc
     6361 atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg
     6421 acgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc
     6481 ccatgcgtcc ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg
     6541 ttcctgcagt tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg
     6601 cacttcatgg cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa
     6661 ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca
     6721 ttcacgcaaa gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct
     6781 ttgcagcgcg cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc
     6841 cacgccgatg atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc
     6901 ggtgattacc gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat
     6961 gatgttgttg gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt
     7021 gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag
     7081 cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat
     7141 attcgccatc tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc
     7201 ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt
     7261 ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc
     7321 cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat
     7381 cttctcaatg accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat
     7441 atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc
     7501 cagtactgcc atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg
     7561 aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta
     7621 atcgccgtta tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa
     7681 acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc
     7741 acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca
     7801 cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt
     7861 gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg
     7921 tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc
     7981 gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc
     8041 gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca
     8101 accggtttct ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg
     8161 cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg
     8221 taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa
     8281 gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc
     8341 agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag
     8401 ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt
     8461 ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa
     8521 tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac
     8581 acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt
     8641 taatccatcc ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg
     8701 taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg
     8761 tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg
     8821 acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga
     8881 aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa
     8941 tcaccaccag cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg
     9001 gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg
     9061 gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg
     9121 cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag
     9181 cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca
     9241 ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca
     9301 aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga
     9361 aatcgctgca agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc
     9421 tgacttctgc caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga
     9481 tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac
     9541 gtcacggtgg cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct
     9601 acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa
     9661 ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg
     9721 gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc
     9781 gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag
     9841 catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc
     9901 tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcg
//
LOCUS       DAZOYL010000136         9933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZOYL010000136 DAZOYL010000000
VERSION     DAZOYL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.136"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(48..123)
                     /locus_tag="ACHUOY_002475"
     tRNA            complement(48..123)
                     /locus_tag="ACHUOY_002475"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88..90),aa:Lys,seq:ttt)
     gene            complement(127..202)
                     /locus_tag="ACHUOY_002476"
     tRNA            complement(127..202)
                     /locus_tag="ACHUOY_002476"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Val,seq:tac)
     gene            complement(352..427)
                     /locus_tag="ACHUOY_002477"
     tRNA            complement(352..427)
                     /locus_tag="ACHUOY_002477"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(392..394),aa:Lys,seq:ttt)
     gene            complement(592..1383)
                     /gene="cpoB"
                     /locus_tag="ACHUOY_002478"
     CDS             complement(592..1383)
                     /gene="cpoB"
                     /locus_tag="ACHUOY_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR0683233.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1393..1914)
                     /gene="pal"
                     /locus_tag="ACHUOY_002479"
     CDS             complement(1393..1914)
                     /gene="pal"
                     /locus_tag="ACHUOY_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR0683234.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1949..3241)
                     /gene="tolB"
                     /locus_tag="ACHUOY_002480"
     CDS             complement(1949..3241)
                     /gene="tolB"
                     /locus_tag="ACHUOY_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR0683235.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3374..4558)
                     /gene="tolA"
                     /locus_tag="ACHUOY_002481"
     CDS             complement(3374..4558)
                     /gene="tolA"
                     /locus_tag="ACHUOY_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR0683236.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSS
                     GKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGK
                     TCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDF
                     KP"
     gene            complement(4623..5051)
                     /gene="tolR"
                     /locus_tag="ACHUOY_002482"
     CDS             complement(4623..5051)
                     /gene="tolR"
                     /locus_tag="ACHUOY_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR0683237.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5055..5747)
                     /gene="tolQ"
                     /locus_tag="ACHUOY_002483"
     CDS             complement(5055..5747)
                     /gene="tolQ"
                     /locus_tag="ACHUOY_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR0683238.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5744..6148)
                     /gene="ybgC"
                     /locus_tag="ACHUOY_002484"
     CDS             complement(5744..6148)
                     /gene="ybgC"
                     /locus_tag="ACHUOY_002484"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0683239.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(6298..6591)
                     /gene="ybgE"
                     /locus_tag="ACHUOY_002485"
     CDS             complement(6298..6591)
                     /gene="ybgE"
                     /locus_tag="ACHUOY_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR0683240.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6591..6704)
                     /gene="cydX"
                     /locus_tag="ACHUOY_002486"
     CDS             complement(6591..6704)
                     /gene="cydX"
                     /locus_tag="ACHUOY_002486"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR0683241.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6719..7858)
                     /gene="cydB"
                     /locus_tag="ACHUOY_002487"
     CDS             complement(6719..7858)
                     /gene="cydB"
                     /locus_tag="ACHUOY_002487"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0683242.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7874..9442)
                     /gene="cydA"
                     /locus_tag="ACHUOY_002488"
     CDS             complement(7874..9442)
                     /gene="cydA"
                     /locus_tag="ACHUOY_002488"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR0683243.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2473 a         2668 c         2369 g         2423 t
ORIGIN      
        1 tacgggtgtt acctgaaaga tttctactcg gggccaatct ttaagattgg tgggtcgtgc
       61 aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact gagctaacga
      121 cccgagtggt gggtgatgac gggatcgaac cgccgacccc ctccttgtaa gggaggtgct
      181 ctcccagctg agctaatcac ccgatactgc gctggatact acaaactact tccaccgaac
      241 ccaccgtaat tagtggtggg tcgtgcagga tgactcgctt cgctcgccct acgggccgtt
      301 gctgacgcaa cgttatccta cacgcttaac atctgtcttc gatgctaaaa ctggtgggtc
      361 gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta
      421 acgacccact ttttcgttgc tttcggtttg tttgatatcc cgtggcaacg gcggcatata
      481 ttactgattt cagacttgag cgcaacaaaa atttcgatgc agatcactca actgcttatg
      541 attcgcacga cacgaccaga aataatgcga cttctggtcg tgtgttatgc attacatcgc
      601 gttcagacgt ttttgtgcct gtttagcgcc atcagtacca gggtatttac tgataacctg
      661 ctggtatacg gctttcgctt ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt
      721 aaacatcgca tcagcagcct ttggtgactt cggatagttt ttcactaccg aagcaaaata
      781 gtacgccgca tcatcttttt tacccttgtt gtagtttaac tgacccagcc aataattggc
      841 gtttggcagg taagttgaat cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc
      901 gtcatcctgg cgggatttat cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc
      961 atcgccgctt ttcaccggcg cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt
     1021 tgatgccgcc gcaccgcttt gatcgctgct ggttgattgc gccgctgcac caccgctgct
     1081 gaggctgtcg atctgcaaca ggatctgctt ttgccgctcc acgacctgat tcagttgata
     1141 ctgattttcc tgaatctgac cacgcaggga atcaatatcg gattgattat cagaaagttg
     1201 ttgctggagt tgggttaaaa gctggctgtg agcattagaa atacgctcaa gttgagtgac
     1261 gcggtcttcg accgagcctg agccgacact actgattggt gcctgagcaa aagcggccca
     1321 gggggccgct ataccaacca gtaacgacag actcaatagt tgatgtctga agttactgct
     1381 catgcaattc tcttagtaaa ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc
     1441 atgacccagt actgcaggtt tttctttacc gtaagaaacg atggagatct ggtctgcaga
     1501 aacgccttta ccctgcaggt acatcttaac ggcgttcgca cgacgttcac ccagggagat
     1561 gttgtattcc ggagtaccac gttcgtccgc gtgaccttct acggtgactt tgtaagacgg
     1621 gttgctacgc aggaagtttg catgtgcatc cagcatttga gcgaagtcag aacggatatc
     1681 gtacttgtcc agatcgaagt aaacgatgtt gttctgctgc agctgttgca tttgcagacg
     1741 agcctgctct tcggaagaca tgttgccgtt gccgccgttc gcatccatac cagtgccggc
     1801 acccagcatg ccttcgctgc cgtcattgct ggcgttcttg ttggaagaac atgccgcaat
     1861 tgccataaca ggcagagcaa tcatcagccc tttcagcact ttgttcagtt gcatttcaat
     1921 gattccttta ctattcaatt aattattatc acagatacgg cgaccaggca gggaatttga
     1981 cctgtccatc agttgccgga agacgcgctt tgaaacgccc atctgtagaa accaaattca
     2041 gcacggatcc catcccctga gaagagctgt agattaccat agtgccgtta ggtgccagac
     2101 ttggcgtttc atccaggaac gtggacgaca gaacttgtac gcctcccgtt gccagatctt
     2161 gtttggcaat gtgctgctgc ccaccgttgg agctgaccat taccataaat ttaccgtcgc
     2221 tgctgacatc cgcatcctgg ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc
     2281 cgttgatatt cactttataa acctgtggac gaccggcctg gtcagaagta aatgccaggt
     2341 tctggctgtc cgggaaccag gtcggttcgg tattgttact gcgaccatca gtcacctggc
     2401 ggatctgacc agaagccaaa tccattacgt acaggttcag gctaccggtt ttcgacaagg
     2461 cgaatgccag tttgctgccg tctggcgaga atgcaggcgc accgttgtga cgcgggaatg
     2521 aagccacctg acgtacagcg ccgtttgcca gcgtttggat aactagcgcg gaacgaccgc
     2581 tttcgaaggt cacataagcc agtttagaac cgtctggtga ccacgccgga gacatcagcg
     2641 gctgtggtga acggtgaacg acaaactggt tgtaaccgtc atagtcagat acgcgcagtt
     2701 catacgggaa ctgaccgccg ttggtctgaa caacgtaggc aatacgggta cggaacgcac
     2761 ctttaatgcc ggtcagtttt tcaaacactt catcactggc ggtatgacca gcatagcgca
     2821 gccactgctt gttcactttg tacgagttct gagcaagtac agtacccggt gcgccgccag
     2881 tgtcaacaag ttgataagca acattgtaag aaccatccgg attcggagtg acctgaccga
     2941 caactacagc gtcaataccc agcgcggacc atgcagctgg ttgtacttcc tgcgcactac
     3001 ccggctgctg tggcagacga gcgcgatcta acggattaaa tttaccgctg ttacgcaagt
     3061 cggcagcaac gatgccgcca atatcttcag gtgccgcacc agggcccgcc cactggaaag
     3121 gaacaacacc aataggacga ccggaatcta caccgctgtc gatcacaatg cggacttcag
     3181 catgcagaac tgatgcccac agtatgagaa aaccaaatgc tactcgtaat gcctgcttca
     3241 tcatatctcc cttatctgga ccgatggccc acgataattt agcagaatgt taacaaacta
     3301 aaatacacaa aactaccaga accccgtggc aaccggtgcc tgatgttgac cgtcggaaca
     3361 gtcaacatcg cgattacggt ttgaagtcca atggcgcgtt tttgaacact tcatatactg
     3421 cctggcttgg tggtttcggg atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa
     3481 gtgcgggatc gccaccttca ggtttgatat ccagtaacat accatcgggt gccagtttta
     3541 tgcgcagcgt acaggttttg cctgcatagg acgatgcgtc atagaactta ctttcgatag
     3601 cagatttaat ctgcccggca tagttattga tatcggcccc tgatgcgcca ttgtttttag
     3661 tattaccact cccggcagac gaagcattgt tccctttcgc ccctcccccc gttttcggtg
     3721 cattcttacc agagcttagc tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg
     3781 cagcggcttt ttcagctgct gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt
     3841 ctgccgctgc tttcttatca gctgctgcct tatcagcagc cgctttttct gctgcttttt
     3901 tgtcggctgc agctttctct gctgccgctt tctttcttgc ttcagctgca gcagcttctg
     3961 ccgcttccgc tttcttcttc agtgccgcag cggctgcctc ggcttttttc tgcgcttcgg
     4021 ctgcggcttt ggcggcttct gcttctgctt ttttcttcgc gtctgccgcc gctttcttcg
     4081 ctgcttcttc cgcagcttta gcatccgctt cggccttcgc tttagcatct gccgccgctt
     4141 tcgctgccgc ctcttccgct tgcttctgct ttaactcggc ctgttttgcg gcttcttcag
     4201 cctgcttttt ctgttcctga gcagctaacc gctctttctc aagttgcttc aggcgttcct
     4261 gttcagccgc ttgtttctca cgcagttctt cagcagcctg ctgttccttt atcttgcgct
     4321 gctcatcaga acgcttcgcg cttgattcct ggctttgcat gcgtttgtac tgctcaacta
     4381 ccgcacctga atcaaccatg acagcgtcga tggacgaacc accgccgcct ccggctgaag
     4441 cttctatatt ctcatcgaac gaactccaga gcagcgccgc aaataagatg acatgcagca
     4501 ctgctgaaat aattatcgcc cgcttgagct tgtcgttttg ttcggttgcc tttgacactc
     4561 tcggtttcca aaaactgttc gcctgttacc cgctctcttt caagcaaggg aaacgcagat
     4621 gtttagatag gctgcgtcat taaaccaacc gatttcacac ccgcactatg taacaagttc
     4681 agtgctttaa ttatttcatc gtaaggcaca tcttttgcgc caccgatcag aaagaccgtt
     4741 ttcgggttgg ccttgaaacg gctggacact tccgccacca cctgctctgg tggtaaacgc
     4801 tccaggcgat ctttctcaac caccacggtg tactgaccaa taccagacac ttcaacaatc
     4861 actggcggat tatcgttact gctcaccgcc tgtgattcag tagcgtctgg cagatcgacc
     4921 tccacgctct gggtgatgat gggcgctgtc gccataaaga tcagcaacag caccagcagt
     4981 acgtccagca acggtacaat gttgatttcg gacttgagat cgcgacgacc tcgtccacgc
     5041 gctctggcca tggcttaccc cttgttgctc tcgctaacgg taaacgcctg gcggtgcaga
     5101 atcgcggtaa actcttccat aaagttgtcg taattcagtt ccagtttgtt tacgcgctgg
     5161 ttgaggcggt tataagccat taccgccgga attgcggcga acagaccaat tgcagtcgca
     5221 atcaacgctt ctgcgatacc gggcgcaacc atttgcagtg ttgcttgttt taccgccccg
     5281 agggcgataa aggcgtgcat gatcccccag accgtaccaa acagaccaat atacgggctg
     5341 atggagccaa ccgtaccgag gaacggaatg tgcgtttcca gattttcaag ttcacggttc
     5401 atggagatac gcatagcacg cgacgcccct tccactacgg cttccggcgc atggctattg
     5461 gcacgatgca ggcgcacaaa ctctttgaac ccgctgtaaa atatttgttc cgaaccagtc
     5521 agattatccc gtttcccctg actctcttga tagaggcgag agagttcgat tccagaccag
     5581 aatttatctt caaacgcttc ggcttcgcgc gccgctgcgt taagaatacg ggtccgctgg
     5641 ataataatgg cccaggatgc gattgaaaaa ccaatcaaaa tcaacatgat aagtttaacc
     5701 agaaggctag ccttcaggaa caaatcaagg atattcatgt cagtcactgc ttaaactccg
     5761 cgacaataga cttgggaagc gcacaaggct tcattttgag tgggtcaacg caaacaacca
     5821 gaacctctgc ttcattcagc aaagtattct cggcgttgac aatacgttgc gtgaaaacca
     5881 aagaggtgcc acgcattgat gttatttcag tctgtatttc gagcatatcg tcgagccgcg
     5941 caggtgcgta atattccacc gtcattttac gtaccacaaa ggcaacgcgt tcagccatca
     6001 gcgcctgctg actgaagtga tgatgacgca gcatctctgt gcgtgctctt tcataaaaag
     6061 cgacgtaact ggcgtggtac accacaccac cggcatcggt atcttcatag tagacgcgaa
     6121 ccggccatcg aaacagcgtt gtattcactt tacatcccgg taatgcaaca aaagttagag
     6181 cttttaaacg ctgctactat acgcgcgcga ttgaggtttg ggaatgaggg caagttaagg
     6241 gagcgtaaaa atttatgggc ctggagcggc ccataatttt ttaaagaaaa ttctgattta
     6301 aaagaagaag aaaatcagcc cgacaatgag aacaatatcg gcaagcaatg ggcaaaaaat
     6361 cccttgccaa agaaccttct gcggacgaaa gcccacgccg tgaatcacac cagcacagac
     6421 ggcccacatc agcaataaac catgccagat ttccagttca ctggtcttcg cggcaaaacg
     6481 agatgggtcc caaaacatac atcctgctaa cagaagcgcc atcacgaagg aaagcgcccg
     6541 cagggggcgc ttgtccatta ccgcatacaa ggtcgcgata atcttactca tcagatgtct
     6601 tcttgaccgg ctttgcctga ttcgacgtgt tcaagcgcca gcgcggtgat taccccaaac
     6661 gaacaggcaa gaagcgttcc cagaatccat gcgaaatacc acatttttag ctccttactt
     6721 agtacagaga gtgggtgtta cgttcaatat cttctttggt gatacgaccg aacattttcc
     6781 agtaacacca agcggtgtag agcagaatga tcggtaccag aaccaccgca acccaggtca
     6841 tgacgttaag cgtcagctgg ctggaagttg catcccacat tgtcagactt gcgttcatca
     6901 tggtgctgga cggcatcaca aacgggaaca ttgcgatacc ggctgtcagg atgatgcagg
     6961 ccagcgtcag ggaggagaac acaaacgccc acgcggcttt atccatacgt gcagtcagga
     7021 tggtcagcag cggaagaacc acacccagtg ccggaatagc ccacagaatt ggcgtgttgt
     7081 tgaagttaac cagccatgcg ccagcttcac gaaccacttc tttattcagt gggttagagg
     7141 ctgcgtaatg gtccattgtc gatttcacga cataaccatc gataccgtac atcacccata
     7201 cgccagccag tgcgaaacag accagtgtca ccagcgcagc cacctgagcc gttgcacggg
     7261 tacgcaggtg cagttcgccc acggtacgca tttgcagata ggttgcgccc tgagtaatga
     7321 tcatccctac gctcaccacg cctgccagca ggccgaacgg gttaagcaac tggaagaagt
     7381 taccggtgta gtacagacgc agatattcat caacgttgaa cggtacgccc tgcaacaggt
     7441 taccgaacgc tacaccaatt accagcggcg gaacgaaact accaatgaag atgccccagt
     7501 cccacatgtt acgccagcgg gtttcttcaa tcttggagcg gtagtcaaaa ccgaccggac
     7561 ggaagaacaa agacgccagc acgaggatca tcgccacata gaagccggag aacgcagcgg
     7621 catagaccat cggccaggca gcaaagagtg cgccgcccgc ggtgatcagc caaacctggt
     7681 taccgtccca gtgtggtgca atggagttaa tcataattcg acgctcggtg tcgttacgac
     7741 cgaggaaacg ggtgagcatg cccaccccca tgtcgaaacc gtcagtgact gcaaaaccaa
     7801 tcagcagaac gccaaccagc agccaccaga taaaacgcaa tacttcataa tcgatcattt
     7861 gacgactcct gtcttagcgt gccggctgag tagtcgtgga agactgctca aagtgatagc
     7921 gaccggtttt caggctgctt gggccgaggc gtgcaaactt gaacattaag aacaattctg
     7981 ccaccaggaa cagggtatac aggccgcaaa tcagcaccat tgagaagatg agatcgcctg
     8041 cggtcagtga cgagttcgcc acagctgtcg gcagcacttc accgatagcc cacggttggc
     8101 ggccatattc agccacgaac cagcccgctt ctacagcaat ccacggcagc ggaataccgt
     8161 acagcgcggc gcgcagaagc cattttttct cgccaatgcg gttgcggatg acactccaga
     8221 aagagagcgc gatgattgcc agaagcagga agccacacgc caccatgata cggaacgcaa
     8281 agtacagcgg cgctacacgc gggatggagt ctttggttgc ctgttgaatc tgcgcttcag
     8341 tcgcatcagc cacgtttggc gtatagcgtt tcagcagcag accgtaaccg aggtctttct
     8401 tcatgctatt gaactggtca cgaaccgcct ggtcggtaga accagaacgc agttgttcga
     8461 gcagagagta cgccttcatc ccgttacgaa tgcgttcttc atgctgcacc atcagctctt
     8521 tcaggccgat aaccggggta tccacggaac gcgttgcaat gatgcccagt gcgtaaggga
     8581 tctgaatcgc aaatttgttc gtctcctctt cctgatcagg aatgccgaac agagtaaagg
     8641 cagcaggcgc aggttgcgtt tcccactcgg cttcaatagc agccagtttg gttttctgca
     8701 cgtcgcccat ttcgtagccg gattcatcac ccagaacaat aacagacaga acagcagcca
     8761 taccgaagct ggcagcgata gcaaaggagc gtttagcgaa ggcgaagtca cgacctttca
     8821 gcatatacca tgcgctgata ccgaggatga acatcgcgcc agtcacataa ccagacgcta
     8881 cagtgtgaac gaatttcacc tgagcaaccg ggttaagcac cagctcggag aagctcacca
     8941 tctccatacg catagtttca aagttgaaat cggacgcgat tgggttttgc atccagccgt
     9001 tcgcaaccag aatccacagt gcggacaggt ttgaaccgag cgccaccagc caggtgacac
     9061 acatatgctg aactttaccc agacgatccc aaccgaagaa gaacagacct acaaaggtgg
     9121 attcgaggaa gaaggccatc agaccttcga ttgccagcgg cgcaccgaag atatccccta
     9181 catagtggga atagtaagac cagttagtcc cgaactggaa ctccatggtc agaccggtag
     9241 ccacacccag agcgaagttg ataccaaaca acttgcccca gaacttggtc atatctttat
     9301 aaatctgttt gccggagagg acgtagaccg tttccataat ggccagcagg aacgccatac
     9361 cgagcgtcag tggcacaaaa aggaagtggt acatcgcggt caaggcaaac tgtaagcgcg
     9421 acagttcgac tatatctaac atcatgactc cttgctcatc gcatgaagac tccgagagtg
     9481 aaccccgtta gaaagggtca cacgcatgcc ccaatagaaa tttgtttgaa cactttcatc
     9541 ataccatgtg aatgcacaca aaaacggcgt aaagaaaggt tgcaaacatg ttaataaaaa
     9601 ctcaaattga tcccacgtat atattacgct gcaaaatcct tacaataaac aggtttttgt
     9661 tgaagcaaat ttgcattttt cgacggtgat caatttatag ctaaattacc gcctttcagc
     9721 caatttgatc gagaacaatt tatctctttt tgatgccaat ttccaagact tatacattga
     9781 taaatatcaa ttcctacaaa ttatgttaat catgtttcaa taaggtaact ttagtgacat
     9841 ttatgtttaa aatgtgtgag ttataaggct gaaattagtt tcggttggca atttaaagat
     9901 aaaaagagac gaagcaaaaa cagcacggtt gac
//
LOCUS       DAZOYL010000137         9895 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZOYL010000137 DAZOYL010000000
VERSION     DAZOYL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.137"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(113..1108)
                     /gene="gapA"
                     /locus_tag="ACHUOY_002489"
     CDS             complement(113..1108)
                     /gene="gapA"
                     /locus_tag="ACHUOY_002489"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0683244.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1450..1863
                     /gene="msrB"
                     /locus_tag="ACHUOY_002490"
     CDS             1450..1863
                     /gene="msrB"
                     /locus_tag="ACHUOY_002490"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR0683245.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1905..2177
                     /locus_tag="ACHUOY_002491"
     CDS             1905..2177
                     /locus_tag="ACHUOY_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR0683246.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="ACHUOY_002492"
     CDS             complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="ACHUOY_002492"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR0683247.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="ACHUOY_002493"
     CDS             complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="ACHUOY_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR0683248.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="ACHUOY_002494"
     CDS             complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="ACHUOY_002494"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR0683249.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6190..6741
                     /locus_tag="ACHUOY_002495"
     CDS             6190..6741
                     /locus_tag="ACHUOY_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR0683250.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6858..7901
                     /gene="selD"
                     /locus_tag="ACHUOY_002496"
     CDS             6858..7901
                     /gene="selD"
                     /locus_tag="ACHUOY_002496"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR0683251.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7906..9867
                     /gene="topB"
                     /locus_tag="ACHUOY_002497"
     CDS             7906..9867
                     /gene="topB"
                     /locus_tag="ACHUOY_002497"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR0683252.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2454 a         2471 c         2633 g         2337 t
ORIGIN      
        1 aacaagtgag cgtttccgga ttcttacaca gccactggat cggtcaactg atccttaact
       61 gatcggcatt acgaccgaag tcgctctttt tagatcacag tttcatctca acttatttgg
      121 agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg tcgtaccagg
      181 ataccagttt cacgaagttg tcgttcagag cgataccagc tttagcatcg aacacggaag
      241 tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag cccagaacgc
      301 ctttcatttc gccttcagca gcggctttaa cggcagcttt gatctgctcg taagttgcag
      361 ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga acgcggaacg
      421 ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct ttagcagcac
      481 cggtagagga cgggatgatg ttctgggaag cgccgcggcc gccgcgccag tctttgtgag
      541 acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc atcagacctt
      601 cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag ttggtggtgc
      661 aggaagcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg cctttaacga
      721 acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca ccagcggtga
      781 tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg acaacgtcaa
      841 caccaacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca cggatttttt
      901 taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa cggccgtgag
      961 tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg ttgattgcaa
     1021 cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg atacggccaa
     1081 aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta gtgaataaaa
     1141 ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg tcaatcattg
     1201 cgacaaatca atcctgtgcc taagcattac gcgactgact cgcctcactc ttcctttggg
     1261 cttgaaacca catggggtcg gcgccccgaa ttttaaaggg caattacgat aaaaatgtga
     1321 ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac aaaattttgc
     1381 cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat taaagtgaga
     1441 tgtgagcaaa tggctaataa accttcggca gaagaactga aaaaaaattt gtccgagatg
     1501 cagttttacg tgacgcagaa tcatgggaca gagccgccat ttacgggtcg tttactgcat
     1561 aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt tcattcccaa
     1621 accaagtatg attccggctg tggttggccc agtttctacg aaccggtgag tgaagaatcc
     1681 attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg ttgcggtaac
     1741 tgtgatgccc atctggggca tgtcttccct gacgggccgc agccaacggg cgaacgttat
     1801 tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga aatcaacggt
     1861 tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat cttgatgaca
     1921 ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt gaactgggga
     1981 aatggcccga tggcgttgcg ttgaccgagg aacaaaaagc gaactgcctg caactggtga
     2041 tgctgtggca agctcgccat aataccgaag cacagcatat gacaattgac acaaatggtc
     2101 agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca gcaaagccta
     2161 ttgctatgtt taagtgaatg agttatctag tatttaagcc tgttattaag ggagggcaaa
     2221 tatacttgtt aggacgtcgt gttttagctg caagtaggtt aaatgcaaca ctgacaagta
     2281 ttatctggcc tacaatgtca ttgaggaaat gtaggccaga gatgaattca actaatatta
     2341 cccctgtgtc tcttcccagt ctgccagcgt atatagcgtt gccccagctg ctgacatctc
     2401 cataaacgcg tgcgcactgt cctggggctg gatattcacg ccacgacaac catcggtaat
     2461 cacatttacc ttataaccta actgtaacgc gtccagcacg gtaaacttca cgcaatagtc
     2521 agtagccagg cccataacga tcaattcatc gatttcatga tcgcgtaacc agtcatcgag
     2581 cgaggttttc tgccgacggc cgttatcaaa aaaggcactg taactgtcaa ctaaaggatt
     2641 ttcgccttta tggaacaccg ctgcgatcgc tttttggttc agtaacggat gtaattgtgc
     2701 gccttcactg ttctgcacac agtgatctgg ccagaaggtt tgtggcaaac cgtcgagttg
     2761 gcctggcgta taaggctcta caccgtgctg actggcaaaa ctgccgtgat tcgccgggtg
     2821 ccagtcctga ctggcgataa ccgcttcacc gcgcgactgg caccagtcaa tcaggcggtt
     2881 agcgacatcc accgtactgt caccttccgg cacggcgagc gcgccaccag cacagaaatc
     2941 attttgtaaa tcgaccagta acagggcgcg agggggcatt acagtctcct taatcatccg
     3001 gcgtcagttc gccgcgcagg ttttggctca tggccttgcg aatggtttca gtatccagtt
     3061 cctggctcag caggtaatgc agtttggtta gcgtggcttc tacagtcata tctgcaccgc
     3121 caataacgcc ggcatgggcg agggcgttac cggtggcata accacccata ttcactttac
     3181 cggacataca ttgtgtcagg ttgaccacca caataccgcg atcgctggct tcttgtaatt
     3241 cctgcaggaa ggctttgttt tgtggcgcat tacccacgcc ataggagcgc agaatcaatg
     3301 ctttcaccgg ttggcgcaga aaattgcgca cgacgtcagc agaaatccct ggataaatcg
     3361 tcactacgcc aattggttgt ggcgtgattg gatgaacgat taattcccct tcaccgtgcg
     3421 gggcgggtgg cgtattcaaa cgacgaatat ggataccagc ttccagtaac ggaggaaggt
     3481 ttggagaggc aaacgcatca aaaccatcgg catgggcttt ggtagtgcgg ttgccgcgat
     3541 acaatcggtt attgaaaaag agcgttactt cgttgatcgg ataattcgcc gcaacgtaca
     3601 acgcattcag cagattaatt tgtccgtcag agcgtaactc agccagcggg atttgtgacc
     3661 ctgtcacaat gaccggttta ccgagattct cgagcatgaa cgacagcgca gaggcagtat
     3721 acgccatcgt gtcggtgccg tgcaggatga caaaaccatc atagtcgtca tagtgcgctt
     3781 taatatcttc agcaatatgc tgccagtctt ctggcgtcat atcagaagaa tccatcagcg
     3841 gcgtatattc atgaatggtg aaatctggca tctccgggcg atggaattcc ggcatcagcg
     3901 ccagttggcg ttgtagatga cctgacaccg gaatataacc ctgctcggaa cgctgcatcc
     3961 cgatggtccc gcccgtgtag gcgacgtaaa ttgatttctt ttgcatgata ttgatatgaa
     4021 tgatatcgaa agagcaaaag tatatacgtg aggcaggagg agcgcacctg atactatgcg
     4081 gataaggaaa tatggggcga gacagcggga agaggattcg tcgacgtaaa aaataatgtg
     4141 gccgacgatc tgtcggccac tcagtacaag acttaacgca cgttggcgca ggtcaggcaa
     4201 aacgcataac ggttttgtgg gtcgttaaac gcggccagct tgtcgctttc acttttaacg
     4261 gtagaggcta ccgaggcaag cggtgcaggt aacatggcct ggaacgcatc tggcaacatt
     4321 gcccggacag aaccagacat gttgtccatc actttgtcga agaaggtcgg ttcatcaacg
     4381 tagtattcca gatgccactg tttcactttt gccagctctg ctgctttggc gaccgcatca
     4441 tcgaaatccc cgagactatc gaccagcccg ttagcttttg catcctgacc ggtccagacg
     4501 tggccctggg cgattttatc aatctgctcc ggcgtcgaat gacgcgcatc agcaaccagc
     4561 gtgataaagc gtttatagcc attctcaatg cttaattgca tcatctgctg cgcttccggc
     4621 ggcagtgccc tggtgataga aacatccgcc agcggtgaag ttgagacacc atcagtatga
     4681 acaccaatcg aatccagact attttctacg gtggtgatca cgccgaagat accgatagaa
     4741 ccggtcaggg tgctggggtt agccacaatg taattagctg gcgtggaaat ccagtaacca
     4801 ccagatgccg ccatgccgcc catcgataca accacaggct tacccgctgc ccgggctgct
     4861 gccagttcag cgcgaatcac ttcagacgcg gtaacgctgc cgcctgggct attaacacgc
     4921 aggacaatcg ctttcacttt cgggtcaagg cgagcgtcgc ggatttgtgc cgcagtggta
     4981 tcaccgccaa cattcccctg agtttcctcg ccatccataa ttgcgccatt agcgaagacg
     5041 acaccgatgc tgtcaccggt atctgccggc gttttcaatg cgtaatcgta ataactgatg
     5101 gcgcgataat ttttatcagt cttactccag ccgaactctt tggttagagc tttttcgatt
     5161 tcggcactcg atgccagtgc atcgaccagc ttgttttcca gtgcatattt cgcggtatcg
     5221 ccaccggttt tggttaaacc ctcaagcaac ccttgcgcgc cagggaatac ctgctgagca
     5281 gggatctgcc ggttagcggc aacagtattc agatagtttt gccacagctc accaatccag
     5341 cggctgtcag cttcgcgggc tgccggtgac atgtcatcac gaataaacgg ttcaacggca
     5401 gatttatacg tacccacgcg gaacacatgg gtggaaactt tcagcttatc cagcaacgat
     5461 ttgtagtaca gaccgttggt agcaaaaccg tgcaaatcca ccacgccttg cggtgacagc
     5521 caaattttat tggcgaaact ggcgagataa tattgcccct ggctgtagtt ctcgccaacg
     5581 gcataaaccg gtttcccgct gtcacgaaac tctttcaggg ctttgccgat gtactgcata
     5641 gacggctggt cgccgcctgc gaagtttttc agatccatca cgataccggt gatattgcgg
     5701 tcgtccttcg cctggcgaat ggtgttgacg atatcaaaca gtgagttttc ctgcagacga
     5761 tcggaactgg caccaagcag ctggcggctt aatttactaa accgctgaga actgtcgggt
     5821 ttatcgacga tcacaccaga aatgtccagc agcagtgcgc cacgactggc cgtttctttc
     5881 gaatcaccac cactgacctg catccagatc cccacaccaa ccagtacgag gaaaataaag
     5941 aacaggttaa gtaccatttc acggacgaaa ttcagcagac gccacgtcca tttaaaaaat
     6001 ccggcaataa atcgccaaag ggttcgcatg tattctccca acttaaggtc acacctgtca
     6061 caggcgcaat agatgtggtt atcgtaatga cacaaccggc acttgtcagc aggaattgcg
     6121 gactgtgctg taacaaaatc cactctcgtg ttaattttgt gaataaatat cacgacagga
     6181 gttaatcaaa tggatgcact cgaactattg atcaatcgcc gtagcgcctc ccgcctggct
     6241 gaacccgcgc caacgggtga acaactgcaa aacatcctgc gtgcgggtat gcgtgcgccg
     6301 gaccataagt ccatgcaacc gtggcatttt tttgtgattg aaggggaagg gcgcgagcgt
     6361 ttcagcgccg tactggaaca gggggcgatt gctgccggta gtgatgacaa agctatcgac
     6421 aaagcccgta atgcgccgtt ccgcgcaccg ctcatcatca cggtggtggc gaaatgcgaa
     6481 gaaaatcata aagtcccgcg atgggaacag gaaatgtctg ccggatgcgc ggtcatggcg
     6541 atgcaaatgg cagccgttgc ccagggattt ggcggcatct ggcgcagtgg cgcattaact
     6601 gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc aggataaaat tgtcggtttt
     6661 ctctacctcg gcacgccgca gttgaaagca tctacgtcga ttaacgtccc ggacccgacg
     6721 ccgtttgtaa cttatttctg ataattgtcg cgaaactgtc tggattgtga gcaaaggcgc
     6781 aggaattcag acactctcac ttatcacttc acggaatgag ggttaccata cccggattgc
     6841 attgacagga gatgtccatg agcgagaact cgattcgttt gacccaatac agccacggag
     6901 ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac catcctgcac agtgagcagg
     6961 cgaagtttgt tgatccgaat ttgcttgtgg gtaatgagac ccgcgacgat gcggcggtgt
     7021 acgatctggg caatggcacc agcgttatca gtaccaccga cttctttatg ccgatcgttg
     7081 ataatccttt cgattttggc cgcattgcgg cgactaacgc catcagcgat atcttcgcga
     7141 tggggggcaa accgattatg gcgattgcga tcctcggctg gccgattaac aaactttccc
     7201 cggaaattgc ccgcgaagta accgaaggtg gacgctatgc atgccgtcag gcgggtattg
     7261 cgctggctgg cggtcactcc atcgatgcga cggagccgat ttttggtctg gcggtaacgg
     7321 ggatcgtacc gaccgagcgg gtgaagaaaa acagtaccgc acaagccgga tgcaaactgt
     7381 tcctgacgaa accgctgggg atcggcgttc ttaccacggc tgagaaaaaa tcactgctga
     7441 aaccagaaca tcagggacta gcgacggaag tgatgtgccg gatgaacatc gcaggcgcgt
     7501 cctttgccaa catcgaaggc gtaaaagcga tgaccgacgt tacgggtttt ggtctgctgg
     7561 gccatttgag cgaaatgtgt cagggcgcag gtgtgcaggc gcgcgttgac tatgacgcga
     7621 tcccaaaatt gccgggggtt gaagagtaca ttaagttggg cgcagtgcct ggcggcaccg
     7681 aacgtaactt tgccagctac ggtcatctga tgggtgaaat gccgcgtgaa gtgcgcgatt
     7741 tgctgtgcga tccgcaaact tctggcggtt tgctgctggc ggtcatgccg gaagcagaaa
     7801 atgaggtcaa agctacagcc gccgagtttg gcattgaact gacggcaatt ggcgaactgg
     7861 tgccagcgcg cggcggtcgt gccatggttg agattcgtta attcaatgcg gttgtttatt
     7921 gccgaaaaac cgagtctggc gcgcgccatt gctgatgtcc tgcccaaacc gcaccggaaa
     7981 ggcgatggct ttatcgagtg cggtaatggt caggtggtga cctggtgtat cggtcacctg
     8041 cttgagcagg cgcagccaga cgcctacgac agccgctatg cgcgctggaa tcttgcggat
     8101 ttgccgatta tcccggaaaa gtggcaatta cagccccgac cctccgtgac caaacaactt
     8161 aacgtcatca aacgcttcct gcatgaagcc agcgaaatcg ttcacgccgg ggacccggat
     8221 cgtgaagggc aactgctggt ggatgaagtg ctggactatc tgcaactggc accggaaaag
     8281 cgccagcagg tgcagcgttg cttgataaac gacctgaacc cgcaggcggt tgagcgggcg
     8341 ctcgaccgtc ttcggtccaa cagtgagttt gtgccattgt gcgtttctgc gctggcgcga
     8401 gcgcgtgccg actggctgta cggcatcaat atgacccgtg cgtataccat tctcggtcgc
     8461 aatgccggtt atcagggcgt actttccgtg ggacgcgtgc agacacccgt gctcgggctg
     8521 gtggtgcgcc gcgatgaaga gattgaaaac ttcgtggcga aagacttctt tgaagtcaaa
     8581 gcacatatcg tgacacctgc cgatgagcgg tttaccgcta tctggcaacc gagcgaagcg
     8641 tgtgaaccgt accaggatga agaagggcgc ttgttacatc gtccactggc ggagcatgtg
     8701 gttaaccgca ttagtggtca accagcaatt gtcaccagct ataacgataa acgggaatca
     8761 gaatccgcgc cgctgccttt ttcgctttcg gcgttgcaga ttgaagcggc aaaacgcttt
     8821 ggtctgagcg cgcagaacgt gcttgatatc tgccagaagc tgtacgaaac gcacaagcta
     8881 atcacttatc cgcgttccga ttgtcgctat ttgccagaag aacattttgc cggacgccac
     8941 gcggtgatga atgccatcag cgttcatgca ccagatctgt tgctgcagcc agtggtagat
     9001 ccagatatac gcaaccgctg ttgggatgac aaaaaggtcg atgcacacca cgccatcatc
     9061 ccgaccgcac ggagttcagc catcaacctg acggagaacg aagcgaaggt ctataacctg
     9121 attgcccgtc agtacttgat gcagttctgc ccggatgtgg tgttccgcaa gtgtgttatc
     9181 gaactggaca ttgccaaagg caaatttgtc gctaaagcgc gttttcttgc tgaagcaggc
     9241 tggcgcacgc tgttaggcag caaagagcgc gatgaagaaa acgacggtac gccattgcct
     9301 gtggtggcga aaggcgatga gttgctgtgt gaaaaaggtg aagtggtaga gcggcaaacc
     9361 cagccgccgc gccattttac cgatgcaaca ctgctttcgg cgatgaccgg gatcgcgcgc
     9421 tttgtgcagg acaaagatct gaaaaagatc cttcgtgcga ccgatggtct ggggacagaa
     9481 gcaacccgtg ccgggattat tgaactgctg ttcaagcgtg gttttctgac caaaaaaggg
     9541 cgctatatcc actctaccga cgccggaaaa gcgttattcc attcgctgcc agaaatggcg
     9601 acgcgaccgg acatgaccgc gcactgggaa tcggtgctga cgcaaatcag cgaaaagcag
     9661 tgtcgctatc aggactttat gcagccgctg gtggggacgc tatatcagct tattgatcaa
     9721 gccaaacgta cgccggtgcg gcagtttcgc ggcattgtgg ctccgggcag tggtggcagt
     9781 gctgataaga aaaaggctgc accgcgtaaa cgtagtgcga aaaaaagtcc gccagcagat
     9841 gaagtcggaa gcggggcgat agcgtaagcg agtgaatctt tcgtgctatt cgagt
//
LOCUS       DAZOYL010000138         9878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZOYL010000138 DAZOYL010000000
VERSION     DAZOYL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.138"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..190
                     /gene="bdm"
                     /locus_tag="ACHUOY_002498"
     CDS             <1..190
                     /gene="bdm"
                     /locus_tag="ACHUOY_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR0683253.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            292..429
                     /gene="sra"
                     /locus_tag="ACHUOY_002499"
     CDS             292..429
                     /gene="sra"
                     /locus_tag="ACHUOY_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR0683254.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            586..2283
                     /gene="maeA"
                     /locus_tag="ACHUOY_002500"
     CDS             586..2283
                     /gene="maeA"
                     /locus_tag="ACHUOY_002500"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0683255.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDIGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2417..3419
                     /gene="adhP"
                     /locus_tag="ACHUOY_002501"
                     /pseudo
     CDS             2417..3419
                     /gene="adhP"
                     /locus_tag="ACHUOY_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3565..3849
                     /locus_tag="ACHUOY_002502"
     CDS             3565..3849
                     /locus_tag="ACHUOY_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0683256.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHUOY_002503"
     CDS             complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHUOY_002503"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR0683257.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHUOY_002504"
     CDS             complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHUOY_002504"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR0683258.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHUOY_002505"
     CDS             complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHUOY_002505"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8080..8082),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0683259.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8899..9780
                     /gene="yddG"
                     /locus_tag="ACHUOY_002506"
     CDS             8899..9780
                     /gene="yddG"
                     /locus_tag="ACHUOY_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR0683260.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2390 a         2593 c         2503 g         2392 t
ORIGIN      
        1 ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcaagg
       61 tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa
      121 cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc
      181 tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct
      241 tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg
      301 aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata
      361 gttaccgaag gtgacaaatc cagcgtggta aataacccaa ccggcagaaa acgccccgct
      421 gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg
      481 gtgtttttgt ttttatctgc tttatacttg gggccgacgc cctggcggta aagcaaagac
      541 gataaaagcg tgccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca
      601 aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg
      661 ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta
      721 ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag
      781 ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga caccaacgaa
      841 accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc
      901 ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg
      961 tttatctctt accagaaccg ccacaatatg gacgatattc tgcaaaacgt accgaaccat
     1021 aatatcaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg tgaccagggc
     1081 atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc
     1141 agcccggcgt atacccttcc ggtggtgctg gatatcggaa cgaacaacca acaactgctt
     1201 aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa
     1261 ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cagacgtgct gttgcagttt
     1321 gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt
     1381 tcttttaacg atgacattca gggcactgcg gcggtaacag tcggcacact gatcgcagca
     1441 agccgagcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca
     1501 gcgggatgcg gcattgccga aatgatcatc gcccagaccc agcgcgaagg attaagcgag
     1561 gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg gcctgctgac ggacaagatg
     1621 ccgaacctgc tgcctttcca gaccaaactg gtgcagaaac gcgaaaacct cagtgactgg
     1681 gataccgaca gcgatgtgct gtcactgctg gatgtggtgc gcaatgtaaa accagatatt
     1741 ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcatccg tgagatgcat
     1801 aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg cgtggaagcc
     1861 acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac gggcagcccg
     1921 tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt
     1981 attttcccgg gcatcggcct gggggttatt gcttccggcg cgtcacgtat caccgatgag
     2041 atgctgatgt cggcaagtga aacgcttgca cagtattcgc cgctggtgct gaacggcgaa
     2101 ggtctggtac taccggaact aaaagatatt cagaaagtct cccgtgcaat tgcgtttgcg
     2161 gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cctctgccga agctttgcaa
     2221 caagccattg acgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc
     2281 taagcctgcg cccggtagtg aaggctaccg ggcaattttc ctctcccttt ttcagatctc
     2341 atccatactg ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat
     2401 ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcatg ttgacgttac
     2461 ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg
     2521 tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa ccgacgtaat
     2581 tctgggccat gaaggcatcg gtgtggtggc agaagtgggt ccaggtgtca cctcattaaa
     2641 accaggcgat cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt gcgaatactg
     2701 taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg
     2761 cggaagagtg catcgtggtc gccgattacg cggtaaaagt gccagatggt ctggactcgg
     2821 cggcggccag cagcattacc tgtgcggggg tcaccaccta caaagccgtt aagctgtcaa
     2881 aaattcgtcc cgggcagtgg attgctatct acggtcttgg cggtctgggt aacctcgccc
     2941 tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc cattgatgtc aatgatgagc
     3001 agttaaaact ggcaaccgaa atgggtgcag atttagcgat taactcacgc accgaagacg
     3061 ccgccaaaat tgtgcaggag aaaaccggtg gcgctcactc tgcggtggta acagcagtag
     3121 ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg
     3181 tcggtctgcc gccggagtct atgagcctgg atatcccacg tcttgtgctg gatggcattg
     3241 aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac cgaagccttc cagtttgccg
     3301 ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt agcggacatc aacaccatct
     3361 ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt gattgatttc cgtcactaag
     3421 aggtctttgc tgcgactccc atgttcgggt cgcagcatcg cacactctcc aacatgaaat
     3481 ggctaaatgg attattgttg cgtaggaagg aacaataatg atagtgttcc cagagaagga
     3541 acgctactga gagtaaagac actaatgaaa atggccaatc atccccgccc gggggacatt
     3601 attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa
     3661 attgcgccct caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg
     3721 gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
     3781 gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgattacg ccgtctggtt
     3841 acgcaataaa aaatggcacc ttataaagcg ccatttgaaa ctgatgacaa acgcaacatt
     3901 gcctgatgcg ctacgcttat caggcctaca cgttcacttc aatctattgt atttgaaggg
     3961 ttttcatgcc ggataaggca ttcacgacgc atccgggatg aacaaagccc acgttgtcta
     4021 caatatgaaa atggcgcctt ttacagcgcc attttcagct taaagttcaa aagagattat
     4081 atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata ccagcgcgga
     4141 tggtgtttct tcgcccagcg acggctcacc ttcccttcga tcatcccttt aatcgaccct
     4201 ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat gatacccgca
     4261 gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg ggaagtactg cgcaaagtac
     4321 ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag
     4381 aacatcatct tttgcccggc gttgtactta ccaacatccg ccactttgtg ctcattgcct
     4441 tttaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg gatgttgtga
     4501 tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa tcgcaatgcc gaagaacggg
     4561 tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg
     4621 aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac ggtccagtga
     4681 caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat gatgatcctc
     4741 ctcgtcatcg tccacttcct tattcgggcc aataccgatg tagtggaaaa tcaacccggc
     4801 aaaagtggca ataaagccag ccgctgccag cggtttcaac gcgcctttcc acaggcttac
     4861 cgaggtgtcg atcttcggat ctttcggcag accgtaatac agctccggct gatcggcgtg
     4921 atgcagcacg tacataacgt gcgtaccacc gacccctttc gggttgtaga cgccagcatg
     4981 ttcgtaacca cgcgctttca gtttcgccac gcgctcttcc gccagctcca gcatctcctt
     5041 cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg gttcctggcc
     5101 gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct ctttgttgag
     5161 gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc agttttccga
     5221 ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg ggcacgcctt
     5281 caggcagccg ggatcttcac agtgcataca gccgtcttta cggatcagcc actccagctt
     5341 gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc tcagatcggc
     5401 ggggttatcg taaaccccga cgcagtgccc cacttcatca cggatgtcgt tccactccga
     5461 acacgccacc tgacaggctt tacagccgat acaggtggaa acgtcgataa gttttgcgac
     5521 ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg cggacctttt
     5581 gataatatcc tgcgtttcca tagccatctg ttcgccccct tacgccttct cgatgttgac
     5641 taagaacgct ttatattccg gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt
     5701 gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggattg gaatacccac
     5761 cgtttcaacc tgctgaccgt ttacattcag cggtttcaga cggcgcgtta ccaccgccac
     5821 cgcgcggata aagccacgct tgcttgagac agtgacacga tcgccattat taatgccttt
     5881 cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcaa ttgcgttgag
     5941 caatgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg tacccacata
     6001 cgggaactgc tcttttttac ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg
     6061 gttagacacc acgttcggat gcagcgggtt agtgcccagc ggcgtttcaa tcggctcgta
     6121 gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc ccatcccttc
     6181 cggctgcatg ataaacggcc cggttggcgt acccggtgcg gcattgccga agtccggaat
     6241 atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg gatcccacgg
     6301 tttaccattg atatccgccg aagcacggtt gtagagcacg cgacggttga gcggccacgc
     6361 ccaggcccat cccagcgtat tccccagacc ggatgggtcg gagttatcgc ggttagccat
     6421 ctggttgccc tgctctgtcc agctaccggt gtagatccag caagaagatg cggttgtacc
     6481 gtcatcacgc agatgcgcaa agctactcag caactgacct ttcttcgcaa tcagcacgcc
     6541 attagcgtca tagagatctt ccagcgcata gccgttattc tctttcgcca cttcgtcaga
     6601 ttgcggttcg tgcggctgct tgtagttcca gctcatcttc atcagcggtt ctacgccttt
     6661 accaccttcg gcctggtaca gctcgcgcag atgatggtag atacccgcca gaatttcgcc
     6721 gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca accagcgacc
     6781 ggagttagcg atagaaccat cttcttcggc aaagcaggtc gaaggcagac ggaatacttc
     6841 agtctgaata gacgccggat cgacatcgtt cgactcgccg tggttctgcc agaaggtaga
     6901 ggtttcagtc accagcggat cgataaccac catgtacttc agcttgctca ggcagctcac
     6961 cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga agtaaccggt
     7021 gactttgcct tcgtccatca tgttgaaata cttgatgacg tcgtaggtct ggtcccactt
     7081 cggcagccag tcatagcccc agttgttctc tttctgcgcg gcatcgccat agaaagattt
     7141 catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat cagccagcgt
     7201 cgctttcggc gtgttcgctt ccagatacga ctgcaaatca acctgttttt ctgacggaag
     7261 cgtcagataa cctggcaggc tggtagagag cagacctaag tcagtcagcc cctgaatgtt
     7321 ggagtgacca cgcaatgcgt tcacgccgcc accggccata cccatgttac cgagcagcaa
     7381 ctggatcatc gccatagtac ggatgttctg cgcacccacg gtgtgctgcg tccagcccag
     7441 cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg gaggccagca cttcacacac
     7501 tttcaggaag tcggcttttg gcgtaccgca gatgttttca acgacgtccg gcgtgtagcg
     7561 ggaaacgtgc gctttcagca ggttccacac acagcgcgga tgagtcagtg tttcatcgcg
     7621 ttttgcatag ccgttttcat cgaactgata gttccaggac gacttatcat actggcgttt
     7681 ttcagcgtcg tagccgctga acaggccgtc ttcgaaagca aaatcatcac gcaccagcag
     7741 gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt ttgttgtttt cgatcaggta
     7801 gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc
     7861 cgccacagaa gcggtacgcg taaaacgagg atcgacaacg atcaaggttg catcgttgtt
     7921 gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag cgttaccgcc
     7981 cattaccatt acgacgttag cgtttttgat atccacccag tggttggtca tcgcaccgcg
     8041 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc
     8101 taccgccagc atcccgaggg agcgggcaaa tttctgggtc agcatcccgg tttcgttgct
     8161 ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta cgccctgctc
     8221 gttcttttca ataaagttag cgtcacggtc agctttcatc agcttcgcaa tacgggagaa
     8281 tgcttcttcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat attccgggta
     8341 gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gccccttttg ggcacagcgc
     8401 accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt
     8461 tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac agtatgtgca
     8521 ggtgttacgg atctctttag cgcgtaataa tttgtagttt cgcgcctgag ccagtgcttg
     8581 cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg cgcagatttt
     8641 aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt attactgcgc
     8701 gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga acacgtgtat
     8761 gccacaaaat aatccgcctg cgcaaattaa cctacctcaa tagcggtaga aaaacgcacc
     8821 actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt taacggcaag
     8881 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
     8941 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct cggtccggtc
     9001 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
     9061 ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt attcgtcagc
     9121 tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca ggcgattgaa
     9181 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
     9241 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt cggcgtctgc
     9301 tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
     9361 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca
     9421 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
     9481 gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt tagcacgccc
     9541 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc tgcatggaat
     9601 gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt tacgcctgta
     9661 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
     9721 ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggttaa
     9781 aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa tattaaaaaa
     9841 taaatttcaa ataccagtaa ttcaccttat atctcatt
//
LOCUS       DAZOYL010000139         9753 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZOYL010000139 DAZOYL010000000
VERSION     DAZOYL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.139"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            425..2056
                     /gene="oppA"
                     /locus_tag="ACHUOY_002507"
     CDS             425..2056
                     /gene="oppA"
                     /locus_tag="ACHUOY_002507"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR0683261.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2142..3062
                     /gene="oppB"
                     /locus_tag="ACHUOY_002508"
     CDS             2142..3062
                     /gene="oppB"
                     /locus_tag="ACHUOY_002508"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR0683262.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3077..3985
                     /gene="oppC"
                     /locus_tag="ACHUOY_002509"
     CDS             3077..3985
                     /gene="oppC"
                     /locus_tag="ACHUOY_002509"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR0683263.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3997..5010
                     /gene="oppD"
                     /locus_tag="ACHUOY_002510"
     CDS             3997..5010
                     /gene="oppD"
                     /locus_tag="ACHUOY_002510"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR0683264.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5007..6011
                     /gene="oppF"
                     /locus_tag="ACHUOY_002511"
     CDS             5007..6011
                     /gene="oppF"
                     /locus_tag="ACHUOY_002511"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR0683265.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6064..6393)
                     /locus_tag="ACHUOY_002512"
     CDS             complement(6064..6393)
                     /locus_tag="ACHUOY_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR0683266.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6428..7888)
                     /gene="cls"
                     /locus_tag="ACHUOY_002513"
     CDS             complement(6428..7888)
                     /gene="cls"
                     /locus_tag="ACHUOY_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR0683267.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8031..8204
                     /locus_tag="ACHUOY_002514"
     CDS             8031..8204
                     /locus_tag="ACHUOY_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR0683268.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8259..9512)
                     /gene="kch"
                     /locus_tag="ACHUOY_002515"
     CDS             complement(8259..9512)
                     /gene="kch"
                     /locus_tag="ACHUOY_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR0683269.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
BASE COUNT         2553 a         2276 c         2423 g         2501 t
ORIGIN      
        1 agacatgctt agccaatctc tatttgattg aattgaaaga tgtttgttaa ggcatggatg
       61 caagctatag attctgatac ggtcaataaa agagaattgc ttaacaattt tgcaaaatgt
      121 attggcgagt aagaaccgca tttggtactt tccgggcaac cgccagacga ttctttattg
      181 gtaatgagaa taattaacaa ttaaagagcg tcgcgaaaga ataatgtgtc tcgacagggg
      241 agacacagta cgaatcgaca taaggtgatc gtctgaatca ccagaataaa taaagtcggt
      301 gatagtaata cgtaacgata aagtaacctg acagcagaaa gtctccgagc ctgtgcaggg
      361 tcccaatccg ggattacaca tgctggttaa taccagtaat tataatgagg gagtccaaaa
      421 aacaatgacc aacatcacca agagaagttt agtagcagct ggcgttctgg ctgcgctaat
      481 ggcagggaat gtcgcgctgg cagctgatgt acccgcaggc gtcacactgg cggaaaaaca
      541 aacactggta cgtaacaatg gttcagaagt tcagtcatta gatccgcaca aaattgaagg
      601 tgttccggag tctaatatca gccgagacct gtttgaaggc ttactggtca gcgatcttga
      661 cggtcatcca gcacctggcg tcgctgaatc ctgggataat aaagacgcga aagtctggac
      721 cttccatttg cgtaaagatg cgaaatggtc tgatagcacg ccagtcacag cacaagactt
      781 tgtgtatagc tggcaacgtt ctgttgatcc gaacactgct tctccgtatg ccagttatct
      841 gcaatatggg catatcgccg gtattgatga aattcttgaa gggaaaaaac cgattaccga
      901 tctcggcgtg aaagctattg atgatcacac attagaagtc accttaagtg aacccgttcc
      961 gtacttctat aaattacttg ttcacccatc aacttcaccg gtgccaaaag ccgctatcga
     1021 gaaattcggc gaaaaatgga cccagcctgg taatatcgtc accaacggtg cctatacctt
     1081 aaaagattgg gtcgtaaacg aacgaatcgt tcttgaacgc agcccgacct actggaacaa
     1141 cgcgaaaacc gttattaacc aggtaaccta tttgcctatt gcttctgaag ttaccgatgt
     1201 caaccgctac cgtagtggtg aaatcgacat gacgtataac aacatgccga tcgaattgtt
     1261 ccagaagctg aaaaaagaga tcccggacga agttcacgtt gatccatacc tgtgcactta
     1321 ctattacgaa attaacaacc agaaaccgcc attcaacgat gtgcgtgtgc gtaccgcact
     1381 gaaactaggt atggaccgcg atatcattgt taataaagtg aaagcgcagg gcgacatgcc
     1441 cgcctatggt tacactccac cgtatactga tggcgcaaaa ttgactcagc cagaatggtt
     1501 tggctggagc caggaaaaac gtaacgaaga agcgaaaaaa ctgctggctg aagcgggtta
     1561 taccgcagac aaaccgttga ccatcaacct gctgtataac acctctgatc tgcataaaaa
     1621 gctggcgatt gctgcctctt cattgtggaa gaaaaacatt ggtgtaaacg tcaaactggt
     1681 taaccaggag tggaaaacgt tcctcgacac ccgtcaccag ggcacttttg atgtggcccg
     1741 tgcaggctgg tgtgcagact acaacgaacc aacttccttc ctgaacacca tgctgtcgaa
     1801 cagctcgatg aataccgcgc attataagag cccggccttt gacagcatta tggcggaaac
     1861 gctgaaagtg actgacgagg cgcagcgcac agctctgtac actaaagcag aacaacagct
     1921 ggataaggat tcggccattg ttcctgttta ttactacgtg aatgcgcgtc tggtgaaacc
     1981 gtgggttggt ggctataccg gcaaagatcc gctggataat acctacaccc ggaatatgta
     2041 cattgtgaag cactaatggc aatacgtggg gcaggagtgt cctgctccac ggtgtctgat
     2101 ttttatcgca ttacagaagg cacaggccag aaggtagggc aatgttaaaa tttattctac
     2161 gtcgctgtct ggaagcgatt ccgacgctat ttattcttat tactatttcg ttctttatga
     2221 tgcgcctcgc gccgggaagc ccttttaccg gcgaacgtac tttaccgccg gaagtgatgg
     2281 ccaatatcga agcgaaatat catcttaatg atccgatcat gacacagtat ttcagctacc
     2341 tgaaacaact ggcgcacggt gatttcggtc catcgtttaa atataaagat tattcggtca
     2401 atgacctggt tgcatccagt tttcccgttt ctgccaaact gggagccgcc gcatttttcc
     2461 ttgcggtaat actgggtgtt agtgctggcg ttattgtcgc attaaaacaa aacaccaaat
     2521 gggactatac cgtgatgggg ctggcaatga ccggggttgt tatccccagt tttgtggttg
     2581 cgccattatt agtcatgata tttgcgatca ttttgcattg gctgccgggc ggtggctgga
     2641 atggtggggc gcttaaattc atgatattgc cgatggtggc gttgtcactc gcttatatcg
     2701 ccagtattgc gcgtattacc cgaggctcta tgattgaagt attacactcc aactttattc
     2761 gtactgcccg ggcgaaaggg ttacctatgc ggcggatcat tttacgccac gcattaaaac
     2821 ctgctctgtt acccgtgctc tcttatatgg gccccgcatt tgtcggcatt attaccggtt
     2881 ctatggttat cgaaaccatt tatggtttgc cggggattgg acaactgttc gttaatggcg
     2941 cattgaaccg tgactattcc ttagtgttaa gcctgaccat cctggttggt gctttaacca
     3001 ttttgtttaa tgccattgtc gatgtgctat atgcggttat cgacccgaaa atccgttact
     3061 gatactggag ctcgcgatga tgttaagtaa gaaaaacagc gagacgctgg aaaatttcag
     3121 tgaaaagctg gaggtcgaag ggcgcagctt gtggcaggac gcacgtcgac gttttatgca
     3181 taaccgtgcg gcggttgcca gtctgatagt gctggtgctg atcgcgttat ttgtaatcct
     3241 ggcaccgatg ctttcgcagt ttgcctatga cgatactgac tgggcgatga tgtccagcgc
     3301 cccggatatg gagtccggtc actactttgg tactgactca tccggtcgcg acctgcttgt
     3361 gcgcgttgcg attggcgggc gtatctcact catggtcggt gttgctgcgg cactggtggc
     3421 agtggtcgtg gggacacttt acggttcgct ttccggttat ctgggcggta aagtggattc
     3481 ggtaatgatg cgtctgctgg aaatcctcaa ctccttccca ttcatgttct tcgtcatttt
     3541 gcttgtgacc tttttcggtc aaaacatcct gctgattttc gtggcgattg gcatggtttc
     3601 ctggctggat atggctcgta ttgtgcgtgg acaaaccctg agtctgaagc gcaaagagtt
     3661 tattgaggcg gcacaagttg gcggtgtatc gacatcgggc attgttattc gccacattgt
     3721 gccgaatgta ctcggtgtgg tggtggtcta cgcatcactt ctggtgccca gcatgatcct
     3781 ctttgaatct ttccttagct tcctggggtt gggtacgcaa gagccgttaa gcagctgggg
     3841 tgcattgctg agtgatggcg cgaactcgat ggaagtctct ccatggttat tgttgttccc
     3901 agcgggattc ctcgtggtga cgctgttttg tttcaacttt atcggcgatg gcttgcgtga
     3961 tgctctcgac ccgaaagatc gttaaggagt gcagccatga gcgtaattga aactgcaact
     4021 gtgccgctcg cacaacaaca ggctgacgta ctgctgaacg tgaaagattt gcgtgtcacc
     4081 tttagtaccc cggacggcga tgtcacggcg gtcaatgatt tgaatttttc tctacgtgcc
     4141 ggagaaacgc tgggcattgt aggtgagtct ggttcgggta aatcgcaaac tgcatttgcg
     4201 ttgatgggcc tgttggctgc caacggacgt attggcggat cggcaacctt caatgggcgt
     4261 gaaatcctca atttgccaga gcgtgaactc aataaactgc gcgctgaaca aatctcaatg
     4321 atttttcagg acccaatgac ttcgttgaat ccctatatgc gcgtcggtga gcagttgatg
     4381 gaagtgctga tgctgcataa gaacatgagc aaagctgagg cgtttgaaga gtcggtgcgg
     4441 atgctcgatg cggtaaaaat gccggaagca cgtaaacgca tgaaaatgta cccgcacgaa
     4501 ttttctggtg gcatgcgtca gcgagttatg attgcgatgg cattgctatg tcgacctaag
     4561 ctgctgattg cggatgaacc cactactgcg ctggacgtca ccgtacaggc gcagatcatg
     4621 acgctattaa atgaactgaa gcgggaattt aataccgcca tcattatgat tacccacgat
     4681 cttggcgtgg tggcggggat ctgcgacaaa gtgctggtaa tgtacgccgg gcgcacgatg
     4741 gaatatggca acgcgcgcga tgtcttttat caacccgttc atccttattc tatcggtttg
     4801 ctcaacgcgg tgccgcgtct cgatgcggaa ggtgaaacaa tgttgaccat ccctggtaat
     4861 ccgccaaacc tgctgcgatt accgaaaggt tgcccgttcc agccacgttg tccgcatgcg
     4921 atggaaattt gtagtagcgc accgccgctg gaagagttta cgcctggccg tctgcgtgct
     4981 tgctttaaac cggtggagga actgttatga atgctgtaac cgaaggaaga aaagtcctcc
     5041 tcgaaatcgc cgatctgaaa gtgcactttg aaatcaaaga tggcaaacag tggttctggc
     5101 aaccgccgaa aactctcaaa gccgtcgatg gtgtcacgct tcgcctgtat gaaggggaaa
     5161 cattaggtgt ggtaggggaa tcgggatgcg gtaagtccac ctttgctcgc gccatcattg
     5221 gcctggttaa agcgaccggc ggtcatgttg cctggttagg taaagagttg ctgggcatga
     5281 agcccgatga atggcgtgcc gttcgcagtg atattcagat gattttccag gatccgttgg
     5341 catcgctaaa cccgcgtatg accatcggcg agatcatcgc tgaaccactg cgtacttatc
     5401 atccgaaaat gtcacgccag gaagttcgcg agcgcgtgaa ggcgatgatg ctgaaagtcg
     5461 ggttattgcc taacctgatt aaccgctatc cgcatgagtt ctccggtggg cagtgccagc
     5521 gtatcgggat tgctcgtgct cttattcttg aaccgaagct gattatctgc gatgagccga
     5581 tttcggcgct ggacgtgtca attcaggcgc aggtggtcaa cctgctccag cagctgcaac
     5641 gtgagatggg attgtcatta atttttatcg ctcatgacct ggccgtggta aaacacattt
     5701 ccgatcgtgt gttggtgatg tatctcggcc atgcggtaga actggggacc tatgatgagg
     5761 tctaccacaa tccactacat ccttacacca gggcattgat gtcggcagtc cccatacctg
     5821 atccggatct ggagaagaac aaaaccatcc agttactgga aggggaatta ccgtcgccga
     5881 tcaacccgcc ttccggttgt gttttccgta cctgttgccc gattgccggt ccggagtgcg
     5941 ccaaaacacg tcctgtgctg gaagggagtt tcagacacgc cgtttcttgc ctgaaagtcg
     6001 atccgcttta aacacaataa gggctgacag ttgtcagccc tttttcacgc cgaaagcgat
     6061 tatttattcc cgccagatga tatggcaaag tttgtgatct ttttcgcggc ataacagaat
     6121 gcgggcgaaa atatcgttga tttcaccatc ctcactgtcc gccagaccaa tcaccacttc
     6181 ggcaaaaaag tccgggttca aatcgaaatc aacatgttcc tgccagtctt ccgccggatc
     6241 gaataactcc gcgccgccgc gctcttcaaa ctgaagattg aacagcagaa catcagctgg
     6301 gtcgagattg tcggcagcca gttcgagaaa aatatcgtag gcctgctcaa gcgtttcatc
     6361 ttcagtcagg cgattgttta gatccatatc catagtcact acctgtttaa cctctgttgg
     6421 cgacgtttta cagcaacggg ctgaagaagt aaaacagtcg ctcggcgaca cgttgccata
     6481 atggacgttt tagccataaa cgggcatcga gcagacgtga acgcgaaata taatcgtcct
     6541 gaacggcggc aaggtctgca ccaaaacctt tatcgtcgat tgccagggta atctcgaaat
     6601 tcagccacag actacgcata tcaaggttaa cggtgccaac cagacttagt tcgccatcga
     6661 ccagcacgct cttggtatgc agtaacccgc cttcaaactg ataaatttta accccagcag
     6721 caagcagttc cgtaaagaat gcgcgactgg cccagccgac cagcatcgag tcattttttc
     6781 gcggaaggat aatactgaca tccaccccgc gctgcgccgc cgtgcaaatc gcatgaagta
     6841 aatcatcgct tggcacaaag tagggcgttg tcatgatcaa atattcacgc gccgaataag
     6901 ccgcagtcaa taatgcctgg tgaatgagat cttccggaaa gccggggcca gaagcaattg
     6961 tgtgaatggt gtgaccgctg gcctgttcaa acggcataat attgacatct ggtggtggcg
     7021 gcagaatacg ttttccggtt tcaatctccc agtcgcagga ataaataatc cccatcgcgg
     7081 tggcgatggg gccttccata cgcgccatca gatcaatcca ttgccctacg cccgcatctt
     7141 gtttgaagta gcgaggatcg accatattca tgctgccggt gtacgcgatg taattatcga
     7201 tcatgatcat cttgcgatgt tggcgcaggt ccatacggcg taaaaacaca cgcatcagat
     7261 tgacctttaa ggcttcgacc acttcaatac cggcattacg cattagctcg ggccacgggc
     7321 tgcggaaaaa agccacactc ccggcggagt cgagcatcaa tcggcaatga ataccacgcc
     7381 gtgcagccgc cattaatgat tcagccacct ggtccgccat gccgccgggc tgccagatat
     7441 aaaacaccat ctcaatatta tggcgcgcga gctggatgtc gcggattaac gcctgcatca
     7501 catcatctga ctcggtcatc agttgtagct gattcccttt gaccccagcg atcccctgac
     7561 gacgctcgca aagcttgaat aatggcgcag cgacactgct attttcttcg gcgaagatat
     7621 gcttacaggc tttaaggtcg ttaagccatt ttgcggtgga aggccacatc gctctggcgc
     7681 gctcagcgcg gcgtttgcct aaatggagct cgccaacggc aagataggca ataattccga
     7741 ctaacggcag aatgtaaata atcaacagcc aggccatcgc ggagggaact gcgcgtcgtt
     7801 tcattagaat gcgtaaagtt acgcctgcaa tgagcaacca gtatcccaga atggccaacc
     7861 aactcaccaa cgtataaacg gttgtcatag attagaaatc cttttgaaag cgcattgtta
     7921 tgagtttacg catatggatt catctggcaa ataaaaacgc gggaaaagca ctggtcagcg
     7981 tcgggggtgg gggtataatg accattctgt tattgcatag agtagttaac atgaagcgga
     8041 gtagaacgga agtggggcgc tggcgcatgc agcgtcaggc tagccgacgt aaatcgcgtt
     8101 ggcttgaggg gcaatcgcgc cgaaatatgc gtatccacag catcaggaag tgcattctaa
     8161 acaaacagcg taactcgtta ttgtttgcga tctacaatat ctaaatatac tgggcaccat
     8221 tgcggtgccc aataagttta ctctttcgtc tttaaactct atttttgcac tgattcttta
     8281 ctgtcagctt tcgtttcttg ttcatcgtta tcgctgaaaa tgccatgacc ggagttcaac
     8341 aacattgaaa cgagcatatc attattaatc tcttcaccat ttaaaactcg cgccagaatt
     8401 tcgctgccaa acagttgcgg tgagagaatg atatccggat gtaccatctt aatcttattc
     8461 aggtttttgc tatcactgac ggcgagaact gttttgacat cactgctcat atcttttgcc
     8521 gagagtacaa caaacgcgtt atctgcatcg ttatcactca gcgccagaat ggcccggcaa
     8581 cgatcgattc ccgctttctt taatactgaa ctgtcattac tgtcaccggg gataacatca
     8641 gcgttatcgc ctaagcgttg ctcaagttgc ttgatatcat cttcaggcaa gttgctgata
     8701 accgttacgt tttgtccgcg ttgattcagt tgcaggatcg tattgatggc gagaatcgaa
     8761 tgtccgcaaa caataaaatg atctttacga tgcattgtat gattgtttcc ttttacaagt
     8821 ttgttgaatc ccccgcggat aagcggacca aaaattgagg tcatagatgt ggcaaaaacg
     8881 gtaatgccgg aaataatgac cgaaatagtg aacaatcgtg ctgattcaga aacagggaca
     8941 atatcgccat agccgacggt tgacatggtt tctatcgaaa aatagaacgc ggtcatcaaa
     9001 ctttctattc gcggattaaa accttcgctt aaataaagcg caccgtaggt tgagtaaaac
     9061 agtaatgtcg tgaaactaat aaatgcaaaa attgtcccgg ctgcggcgct actgtgggag
     9121 aagtctttgc gcagtatcag caaaaacacc agcgtaaaaa tgcaaaatcc aatactaaat
     9181 ttcagccagg gataaaaatg cagggtgtaa attagcgcta tcaacaacaa aatgatactg
     9241 attgcccatg cgagctttgc ccggaacagt aagccaatgg agttcaggac aagaaagacg
     9301 ccgagcataa atagtggggc gtttgccagc aaggagagat ccaactctga aaatgctttg
     9361 atatggaaaa tatcaagcaa attgaccgac ataccataga ttgttttaaa aataagcaat
     9421 ccatttaaaa agacggccag cgcgagaatg tcgtgccgaa gggtcaccca taaatttgtt
     9481 gctgtttgtt tgaatgtagc ccagtgactc actgatacct ctgtaaccac aattttgttt
     9541 aattatctta gcgtgcacat tttctgaaca tacatgcagc gcgtgaatgt gttaaacaat
     9601 agtagatgac tttatccgca gtaatcaaag gatttgagcg agcaactgta cctcaggaaa
     9661 ggtcattttt cctgaatatg ctcacatcat ataaagaaat acagataaag ttattatctg
     9721 cttgtggtag tgaatgcact gaccggctat aag
//
LOCUS       DAZOYL010000140         9731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZOYL010000140 DAZOYL010000000
VERSION     DAZOYL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.140"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHUOY_002516"
     CDS             267..1925
                     /locus_tag="ACHUOY_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR0683270.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHUOY_002517"
     CDS             complement(1922..2878)
                     /locus_tag="ACHUOY_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR0683271.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHUOY_002518"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHUOY_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR0683272.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHUOY_002519"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHUOY_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR0683273.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUOY_002520"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUOY_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR0683274.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUOY_002521"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUOY_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR0683275.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUOY_002522"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUOY_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR0683276.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUOY_002523"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUOY_002523"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR0683277.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9343..>9731)
                     /locus_tag="ACHUOY_002524"
     CDS             complement(9343..>9731)
                     /locus_tag="ACHUOY_002524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683278.1"
                     /translation="RARTKVRAFFSHFIFLYGSEHGGRVEYVTCEFLSCLYGSERVRK
                     GFTAPRRFLSCLYGSELKYYTFSPLLIKQNHLIFLLSPFFILTFSSSTNYQQITFTSK
                     NRVFTVPPHKQKVRNLFRDYGLHQPT"
BASE COUNT         2384 a         2275 c         2704 g         2368 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt c
//
LOCUS       DAZOYL010000141         9646 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZOYL010000141 DAZOYL010000000
VERSION     DAZOYL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.141"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..669)
                     /gene="ydcF"
                     /locus_tag="ACHUOY_002525"
     CDS             complement(<1..669)
                     /gene="ydcF"
                     /locus_tag="ACHUOY_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR0683279.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDS"
     gene            complement(941..4843)
                     /gene="hrpA"
                     /locus_tag="ACHUOY_002526"
     CDS             complement(941..4843)
                     /gene="hrpA"
                     /locus_tag="ACHUOY_002526"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR0683280.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5044..5649
                     /gene="azoR"
                     /locus_tag="ACHUOY_002527"
     CDS             5044..5649
                     /gene="azoR"
                     /locus_tag="ACHUOY_002527"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR0683281.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5700..7016)
                     /locus_tag="ACHUOY_002528"
     CDS             complement(5700..7016)
                     /locus_tag="ACHUOY_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR0683282.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7006..8763)
                     /locus_tag="ACHUOY_002529"
     CDS             complement(7006..8763)
                     /locus_tag="ACHUOY_002529"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR0683283.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8779..9646)
                     /locus_tag="ACHUOY_002530"
                     /pseudo
     CDS             complement(8779..9646)
                     /locus_tag="ACHUOY_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2448 a         2628 c         2274 g         2296 t
ORIGIN      
        1 gctatcatcg cgtaaacgcg gcagctcgcc agtgagtagt gagagataac gctcaaccgg
       61 ccataatcct tgtaactgat taataaagat tacactgtct gcgttatttc ctaactgagg
      121 aacgaatccg ggataactta accagcgtgg tgcatcggga ttgtccccag tcatacggcg
      181 gaacgtcgcc atcgtgcgcc gctgcatggt ggggtcctga acaacgatag ccgtatgaac
      241 tcgttctacg gcctgattca atagcgcgat gctaaagcgt gcgttttcac cgcagtttgt
      301 tgactggtct tcaatccaga ttttttcatg cggaatgtgc cagaactgat gagcgatatc
      361 cgcctggatg gtcgcttctg ctctgccagt ggtgcggata gtgttgtagt gcggatgctg
      421 tgcgatggcg ctatacaaaa aagttgtcga gtgaccgata ccaccactaa tcagtaaagg
      481 aatttgctga tcgcgagcaa tcttacatgc cgcgtcgata gtcggcataa ccgcattgcc
      541 tgcaaggatc acgcaatcgg cctgatacgg cacctcaccg gagaaatcat cctgcgccag
      601 ccactgtccg ataacattta tggcatctat agttgccggc gaaagcgtcg gaaacggggt
      661 aatgttcatg gttcctcctt atcgttccct taagaatact gcgtgagata atagtgagaa
      721 gcggagcact ctgaaaggag gaagtcgtat ttccttaacc gccactccgc aagcctgaca
      781 tatcttttaa ggaaatgctg tagataaaca ttattcatct tgcagcagag aattgtattt
      841 ttctgaattc ctgaattttt acccttaccg ttttagaatt aataaaaaac ccggcgctaa
      901 atgcttaccg ggttttttct ttatcaggca aatagcaggg ttaaccgcta atctgctcca
      961 tcgcctgcaa aatacgctta tctgaaatcg gataaggcgt accaagttgt tgagcgaagt
     1021 aactaacacg caactcttct atcatccaac ggatctcttt cacgtcttca tcctcacgac
     1081 gtgcaggcgg cagtttgttg atccattgct gccacgcctg ctggacgttt tcgactttca
     1141 gcatctgagc acggtcgcga tgtggatcaa ccgccagttt ttccagccgt ttttcaatcg
     1201 cctgcaaata tcgcagcgtg tcgcccagcc gtttgaagcc gttaccagtg acaaaaccgc
     1261 gatataccag cccgcccatc tgcgctttaa tgtcagaaag ccccagcgcc atcgtcatat
     1321 ccacccgccc cttcagacgt ttgttgatat tgaacactgc cgtaaggatt tgctcgacct
     1381 gcttcgcaat atccaccacc gtgtcgttca gttcggcacg cactttttca tgcagcgcag
     1441 caaagccttc ttccgtccag accgggccac cattggcgtc gatcaattta tccacaccgc
     1501 aggagataca gtcgtcgatc agctccagca ctttgccata cgggttaaag tacagcccca
     1561 gcttggcttt gtttggtaac ttctcatgta aatacttgat cggcgaggga atattcagca
     1621 gcagtagacg gcgaagaccg ttccacatta cctgtttttg ctccagcgga ttatcaaaca
     1681 gtttgatcgc cacactgtcg cgttcatcca ctagcgctgg ccacgccttc actttgtagt
     1741 tgccacgctt ctgttcgtag ctttccggca gctgaccaaa actccagata tgcagtccgc
     1801 tctgctcgat accgtcatcc gccaccgcag acagcgtttc ctgcactttg cctttcagcg
     1861 catctttcag atcttgtagc gagcgccctt cttttagctt cttgtttttg tcatccacca
     1921 cgcggaaggt gattttcagg tgatcgggca cctgatccca gtgccagtct tcgcggtcaa
     1981 cggtaacgcc ggtcatccgc cgtaactcgc gctcaaggct gtcgagcaac ggtaactcca
     2041 gcggtgtgac gcggcctaaa aacgcttcgg cgtagtttgg tgcaggcaca aaattacggc
     2101 gtaccggttt cggcaacgat ttaatcagag caatcaccaa ttcgcggcgc agaccgggga
     2161 tctgccattc aaacccgctt tcctcaacct ggttaagtaa cggcagcgga atatgtacgg
     2221 tcacaccgtc cgcgtccgcg cctggctcaa actgatagct caaacgcagc ttgagattgc
     2281 cctgatgcca gaagttcggg taatccagct ttctgatttt ttccgcccct tctttgatca
     2341 acatgctctt ttcaaagttg agcaaatcag gcgtttcgcg gctgactttt ttccaccagc
     2401 tatcgaagtg acgagcggag attacatcgt ggctgatgcg ctggtcgtag aactcaaaca
     2461 gcgtttcgtc atcaaccaga atatcgcggc gacgtgattt gtgttccagc tcttccactt
     2521 cggcccgcag tttcaggttt tcacggaaga atgcgtgacg cgtctgccag tcaccttcca
     2581 ccagagcgtg gcgaataaag agttcacgac ataacgccga atcgatctgg ctgtagttga
     2641 ccttgcgcgc agcaacaatc ggcaaaccat aaacagtgac tttttccgtt gccatcaccg
     2701 cgccctgcgc ccgttcccag tgcggttcgc tgtaggtgcg tttaatcaaa tgctgagcaa
     2761 cgggttctac ccattccggg tcgatacgcg cagcaatgcg cccccacagg cggctggttt
     2821 ctaccagttc cgccaccatt acccatttcg gcggcttttt gaataaacca gaaccgggga
     2881 agatggagaa acgcgcgtta cgtgcaccgg tatattcctg tttatcggca tctttcatgc
     2941 cgatatggga aagcaaaccg gtcagtaagg cgatgtgaat ttcacgatac tccgccggtt
     3001 cgctgttgac cggaatgcca agttctttca ccacctggcg caactgggtg tagatatcct
     3061 gccattcgcg cacgcgcaga tagttgaggt aatcggtacg acacaggcga cggaaggcgt
     3121 tggaagaaag cgccttttgc tgctcgccaa gataattcca cagatttaca aacgcgagga
     3181 agtcggattc tttgtcgtgg aagcgacgat gtttttcgtc cgatgcctgc tgtttgtcca
     3241 tcggacgttc gcgcggatcc tgaatggaga gcgcggacgt gataatcatc gcctcacgca
     3301 cgcagccatg tttttgcgct tccagcacca tacgcgccag acgtgggtcg acaggcaact
     3361 gcgagagctg gcgaccgagc ggcgtcagtt tataggcgct ggcctgttca tccgtggtga
     3421 tcgcgcccag ttcttcgagc agacgcacgc catcctggat attgcgttta tccggtgctt
     3481 cgacaaacgg gaacgcagcg atatcgccca gccccagcgc ggtcatctgc aaaataaccg
     3541 aggccaggtt ggtacgcaga atctccggat cggtaaactc cgggcgcgag aggaaatcgt
     3601 cttcggaata gagacgaata cagatccctt cggacacacg accacagcgg cctttacgct
     3661 gattggcaga cgcctgcgaa atgggttcaa tcggcaaacg ctgcactttg gtgcgatagc
     3721 tgtagcggct gatacgcgct gtaccggggt cgatgacgta tttaatcccc ggtacggtca
     3781 gcgaggtttc cgcgacgttg gtcgccagca caatgcgccg tccgctgtgc gactggaaca
     3841 cgcggttctg ttcgctgttc gaaagccgcg cataaagcgg caagatttcg gtatggcgta
     3901 agttcagctt gttcagcgca tcggcggtat cgcgaatttc ccgctcacca ctcataaaga
     3961 tcagaatgtc gccagggctt tcctgactca gttcgtctac ggcgtcaaaa atcgcctgca
     4021 actgatcgcg ctcggtgtca tcggcttctt caacaatcgg tcgatagcgc acttccaccg
     4081 gataggtccg accggagact tcgataatcg gcgcattatt aaagtggcgc gaaaagcgtt
     4141 ccgggtcgat agtcgcggaa gtgataatga tttttaggtc aggacgccgc ggcagcaact
     4201 ctttcaaata gccgagcaga aaatcgatat tcaggctgcg ttcgtgcgct tcgtcaataa
     4261 tgatggtgtc gtactgcatc agcaggcggt cttgctggat ctccgccagc aggataccgt
     4321 cggtcatcag cttgaccatc gtgttatcac ttacgtgatc gctgaaacgc actttgtaac
     4381 cgatgcaacc gcccggctcc gttttcagct cttccgcaat acggttcgcc accgttcttg
     4441 ccgccaggcg acgcggctgg gtatggccga tcagcccttt aatcccgcgc cccagctcca
     4501 tacagatttt cggtaactga gtcgttttac cagaacccgt ttccccggcg acgatcacca
     4561 cctggtgatc acgaatcgct tcgagaatgt cctgtttttt ctgactaacc ggtaaattgt
     4621 caggataagt aatttccggt cgtgccgctt cacgcagcag gactttccct gccgcctggt
     4681 caatctcttt cgccatctcc tggaaaatgg cctgttgtgc atcaggattt ttaaccttct
     4741 tcacgccgtg cagacggcga gaaaaacgca gtctgtcacg cagcatcagc gaatccagcc
     4801 gctgctgcaa ggccgtaaag gtcaattttt gttgttctgt cataacgtta gtgggcagta
     4861 gtctgcctga ttaattttct ttgaaatgat agatatagag taccacatca cggctttcta
     4921 tgcgttgttc aataaattcg aacataggct tcgatttatt gcgctatctc tgtggcagga
     4981 ttgtgaataa agtgtcaaca agcaacgggg catcgcccat tcaaacatct ataaggaaac
     5041 accatgagca aggtattagt tcttaaatcc agcatcctgg cagggtactc tcagtctaat
     5101 cagttgtccg attattttgt tgaacaatgg cgcgaaaagc actccgctga tgaaatcacc
     5161 gttcgcgacc tggctgcaaa tccgattccg gtactggatg gcgaactggt tggtgcactg
     5221 cgtccgagcg atgcgccgct gactccgcgt cagcaggaag ctctggcgct ttccgatgag
     5281 ctgattgccg agctgaaagc ccacgacgtt atcgttattg cggcaccgat gtataacttc
     5341 aacatctcaa ctcagttgaa aaactatttt gacctggttg cacgcgcagg cgttactttc
     5401 cgctataccg agaacggtcc ggaaggtctg gtaacgggta aaaaagccat cgttattacc
     5461 agccgcggtg ggatccacaa agatggacca acggacctgg tgacgccgta tctgtccacg
     5521 ttcctcggct ttatcggcat tactgatgtg aaatttgtct tcgccgaagg gatcgcatac
     5581 ggtccggaaa tggcagcgaa agcgcagtct gacgcgaaag cagccatcga cagcattgtt
     5641 gctgcataac cggacacgat cttgatgtgg actgtagtcc agtccacatc ccaatccact
     5701 tacctgtttt cccataattt cagcatcgct ttgtgatctt ccttaagcac aatatgcgag
     5761 cgtctggctc gaataaagct aatcgcttca tcaacggttt tacagtatcc gtaacataac
     5821 agccatgccg ccactaccag cgcactgcgc gataatccca gtgcgcaatg gaccagaacg
     5881 ctgccttgct cttcgcgtaa tgtttccagc atcgccacgg cctgtcggag ctccccctct
     5941 tccggaacca ccagatctag catcggtaca cagaaataga gtcgatcttt tgtcgctcgt
     6001 ccacgaggga attcaaaggt gacgtccaga accgcattct gtgccggaat atgtcgtgga
     6061 aacgccccta aataaacacc agcagtcatt ttgctcaccg gctccaggcg acgacaaaac
     6121 caacgcatag atagccacat cccgatgcgc cagggcaatg tcagccagta aacggcgggc
     6181 gggagtttcc cctgactatc tttccctgtt gttatcgcgc caagcccacc gtacccacgg
     6241 ccaataatga gtagcgataa tactggccaa cataaccaga ctgaccacca taactgaacc
     6301 atcatcatta gctccatcaa cacaatgcac gcgcacgcgc ctacgacata tggcagtgct
     6361 attttgattc gacgttgatc aggtttctga taattccaac gacggtcgac gggcaccatc
     6421 cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg atgctgccat
     6481 gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cgcagacttt acgccacctc
     6541 acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat atgcagcgaa
     6601 ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca tcccgtcacc
     6661 ccactcactt caggacggat aaaactaaac ttcagcggat agagcaaaaa accgcagcag
     6721 gccattaccg ttgccagaat aagccggtgg acaagtcggc gctgttcgaa tgtcgtgcta
     6781 caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac aatagtccag
     6841 ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tgggaatatc atggctatta
     6901 ctgtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa aaacggggcc
     6961 agcaacaata accagccagc tccttgtagc aatacgttcc ggcgttcagt tatcacgacg
     7021 caccgccatc gaaaccgtaa aaatgcccca ctcatcaatc cgttgtgtgc atttatcaaa
     7081 tccagcatca tgcacgagca aatccatctc cccttgcgta cgcacgcgca ttacccacag
     7141 tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg ggtgccacgg
     7201 ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggcaagac ctgcgagtga
     7261 gttttttacc tgctcatttt cgggaaacag ctcatacagt ccagagacaa tcgccagcgt
     7321 aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct gttcaaaacg
     7381 cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa catttaactc
     7441 actgtaatca cgtaataaaa tatcgcttac ggcaggctcg tttgccagtg catccagtac
     7501 atagcgccca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt tgacgtggag
     7561 atcggcaacg gcttgtttaa tcagtatttg taaatgagtt ttgcgctggc gaataccgcg
     7621 ccagccaaca ctgttcaggt aaattttgtc gattaagcgc ccgaatgcgt tactgccttg
     7681 cggttgattt tgatagacat aatccagcga actgcctgaa tcaaagccag tgctcattcc
     7741 gagatgcagg cccgcagagt gcgtcccgaa caatttcatc gctttacgca taaagcgata
     7801 cgccaaatca accggcgata atggcacggg tccacctgaa aggaggcgcc agcgatccgc
     7861 tgatggtcca gtgcggtctt catgctgata atcaaatttt tgtgacttgt tagcatataa
     7921 acgacgaata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt cacccaacgt
     7981 gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta atctctgata
     8041 aaaatcaatc tgcggttggc gatgcaccac atagtcatcg cctgatatca gaagttgcgt
     8101 ggggagcgta atcgccgccg catctctaat aatacgttca gacgttttat agagatcgag
     8161 caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac tcgccacccg
     8221 ttgccgatcg tgggtcaaat agcgtctttt cacataggaa ttaataaaaa acagaccacg
     8281 cagacgatgc catagcgtca gcgcaggacg tgccagcggc acatacaatt taaccttaaa
     8341 ggccggagaa gccagcacca gcccgcgaat tgcaggcgca taatcatgaa cccatgtggc
     8401 aaccagcact gcgccgacgc tttgcgcgat cacaaccacc tcttccagtc caacctggct
     8461 gtcgctggca gcaaaacgga caaattcatc gacatcctgc actgaacgcg caagagatgg
     8521 gctataacca cgcggccccg aagtttttcc atgcccccgg gcatcccatg cataaaaagc
     8581 agtatctggc atcgccagtt catcaacgat atgttgtaga cgaccagaat gttcatggcc
     8641 acgatgaaat aagacgatga cctttttcgc ccctggctgt aaagcgggcc agtgccgata
     8701 aaacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagagatgc gtgaattttc
     8761 cattttctct attttccttc agtaacagca gtaacgaata aaataaaaaa ataccggagc
     8821 ggtgaaaatt aatgagtcaa tcctgtcaag gattccgcca tgtccaggta atagtttccc
     8881 gctatcttta acaccaacat ctcgtttgat ggctgacatc actacatcgc cgcaaaaacc
     8941 actaatacca attaacaaac ccgctaatat tgcctgtaat gtatttagcg gtgtcagtaa
     9001 cggtccaatg atcatcgacg ctatcatggt ggtgattacg ccgccgacca gaccttccag
     9061 cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt tcccccataa
     9121 atactgtgca atatcgtttg acgcggttaa tgccaaaaga aataacacca gtaaggcccc
     9181 gccctggata ttcatggatg gcaacattaa taactaggcg gcatgactca aggcgaaaac
     9241 ggtcattatc cagccccaaa aaatggcgct cacggtatgc agaaaaccgg aggggtcacc
     9301 cacaaaaact cgccaggcgg ctaatatcag aaacccagcc aaaggtataa ataacagaaa
     9361 cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc aatataacca
     9421 ccgcggaaaa tgtacggata taagcataca atattctttc aatgccagaa aacttatcag
     9481 cgcaaaaaac gccaacgtca tccagcgtgg acctgaaatc accagtgaaa aacaaatgac
     9541 gatgccccac caggtacgaa tacgtaatgt aacctcaccc ccttttctct ccggtaaccg
     9601 ccatagcaga aaacggttta tcagcgttgc taaaattaac aacgcg
//
LOCUS       DAZOYL010000142         9646 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZOYL010000142 DAZOYL010000000
VERSION     DAZOYL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.142"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..541
                     /locus_tag="ACHUOY_002531"
     CDS             <1..541
                     /locus_tag="ACHUOY_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR0683284.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
     gene            complement(597..1139)
                     /locus_tag="ACHUOY_002532"
     CDS             complement(597..1139)
                     /locus_tag="ACHUOY_002532"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0683285.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1123..1434)
                     /locus_tag="ACHUOY_002533"
     CDS             complement(1123..1434)
                     /locus_tag="ACHUOY_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0683286.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(1619..2509)
                     /gene="cyoE"
                     /locus_tag="ACHUOY_002534"
     CDS             complement(1619..2509)
                     /gene="cyoE"
                     /locus_tag="ACHUOY_002534"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR0683287.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2521..2850)
                     /locus_tag="ACHUOY_002535"
     CDS             complement(2521..2850)
                     /locus_tag="ACHUOY_002535"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR0683288.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(2850..3464)
                     /locus_tag="ACHUOY_002536"
     CDS             complement(2850..3464)
                     /locus_tag="ACHUOY_002536"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR0683289.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3454..5445)
                     /gene="cyoB"
                     /locus_tag="ACHUOY_002537"
     CDS             complement(3454..5445)
                     /gene="cyoB"
                     /locus_tag="ACHUOY_002537"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR0683290.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5467..6414)
                     /gene="cyoA"
                     /locus_tag="ACHUOY_002538"
     CDS             complement(5467..6414)
                     /gene="cyoA"
                     /locus_tag="ACHUOY_002538"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR0683291.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(6875..8350)
                     /gene="ampG"
                     /locus_tag="ACHUOY_002539"
     CDS             complement(6875..8350)
                     /gene="ampG"
                     /locus_tag="ACHUOY_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR0683292.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8394..8972)
                     /locus_tag="ACHUOY_002540"
     CDS             complement(8394..8972)
                     /locus_tag="ACHUOY_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0683293.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9277..9594
                     /gene="bolA"
                     /locus_tag="ACHUOY_002541"
     CDS             9277..9594
                     /gene="bolA"
                     /locus_tag="ACHUOY_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR0683294.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
BASE COUNT         2640 a         2418 c         2412 g         2176 t
ORIGIN      
        1 gggcattatg tacgccaatg gtcagtatgt tgaggtagat tgtaagaaag cgaaggaata
       61 tcttgataaa ggtgtccaca tttatggtgg cccggaagac tttctggcta cttgtcgaaa
      121 agacatgatt gacagaaaaa cggttgacga tacgttaccc gtgattacgg ttacccgttc
      181 tgggatgcga gataattttt tagataaagg tttttcctgc atggatagcc tcttcgccac
      241 caccaataaa ttaggcgaag tggccaatct tcgcgtgaca ttcagcattc gtcgcccatc
      301 cggtaaggag attaaccaaa cggttggctt cgcgcctttc ggtttaaacc ggctgaatat
      361 tagctttact gattacctat ttggttcatt taccagtaat tcgtctctta ttctttataa
      421 accggaattt gagcgaaaat cttgtgcaac tgtaaggacc acaatcgttg ccgcaacggc
      481 aaccattaac ggcaaggatg tggagttgct gaaagcgggg gcaattgaac aaaagtggta
      541 acgctgctca gcccccggct aaggcaaaac ttgccctggc gggtggcgat aataatctaa
      601 gccgcgagaa tatgctgaat cgccaaaaac ataggtacag caccaaaagc atttggtgtc
      661 gctgaaagct gaacaaagcc gagacgagca taaaaattaa tggcggcagg gtcagcatca
      721 agataaaccc ctttaattgg taatgcctgg tgaattattt ttacatgttc aaaaaaatca
      781 catagtaggt cctgaccaaa gccccgcttt tgatacttcc gtgctacccc caacatgact
      841 aatctgacaa caccaatttc tgaaggttgt gaaccctgaa ggacgccaga aacgcgttgt
      901 ttttccagcg aatatgccgt aaaagtacag atgccgatca gttcaccact ttgtctgtca
      961 ataagtgctt tagccgcaca gtcgctatta cgcacacttt tctttagcga tgcgcgtaca
     1021 aatttatcga taacagggtt accgcaatca aactgttttt gcccaggata agtaatatca
     1081 gactgataat tacaggagag gactatgggc agagtaatct cttcatgctt gtcgaccatc
     1141 gctgtttttc cttttcatta acgccttgag ggcgagcgtc ggttgagcag gttcagcgat
     1201 gagttggttc atcagttccc agctttgatt cgaaagctca cggcgacgtt ggttatcaag
     1261 cacactttcg gcgcgttcca tagcagcgct caaaataaaa gcgcttaaat ctaccccaac
     1321 ggcttgcgca gcttcacgaa gtttgttttt ggcgtcggga ctagttttga gttcgacccg
     1381 cgcatttttc agcgttttat cgacatcggg tagagtggct gtttttccag gcattatgac
     1441 ctctttgatt aactgaatga aatcgtaggg agtcgatctc atacataata cggctatcaa
     1501 ccgtatgatt aatttatgtt catctgctca ctctgtcaag acatacggtt attagccgta
     1561 tctctgatct cagacaataa aaaaggtgcc gtagcacctt tttaatagag aggttgtgtt
     1621 accaaacagc agccagcagc gtatgcgagt ccggcaccat aaaatcaacg gacatcatca
     1681 ccgagagggc tgtgatggcg atgatagaga agccgaacag cttgcgcgcc cagatcctgt
     1741 catcagcaac tttataaccg cgcagggcca tacctaacca ccagacgctc accgccgcgg
     1801 cgaccaccag atatttatac ccagcgtaac cgccaagaga gagcatcagc gtggcaacgg
     1861 caaaggcgat gatatacagc gtgatgtgat tcttcgccac cgaaatgcct tttaccactg
     1921 gcaataccgg aatgtttgcc gcctgataat ccttaaagcg gaaaatggcg atggcatagg
     1981 agtgaggcat ctgccacagg ctgaagatag ccagcaggat cgctgcgccg ctatcgaact
     2041 cgccggttac tgcacagtag ccaatcaccg gcggcgcagc gccggagagc gaaccaatca
     2101 atgtgccgta gaccgagtgg cgtttcatgt acaggctata aacgccgaca taaaccacaa
     2161 agcccatcac ccccagccag caggccagcg gattcgcgcc aaaccacagc agcataaagc
     2221 cagcaaagcc tagcaaggtg gcgtacacca gcgagacagc aggagagatc aggcctttaa
     2281 ccagcacccg attcttcgtc ctttccatct ttctgtcgat atccctgtcg atgtagttgt
     2341 taaacacaca acccgacgcc acaaccagtg acaccccaac cagcgtgtag ataaacaggg
     2401 gataatcaat gctgcccttt gaggccagca ggaatccccc aatcaccgag atcaggttgc
     2461 caaagatgat gcctggtttc gttacttgca ggtattgctt aaacatcata accgccgctc
     2521 ttagtgcatc atcatgttgt agttgaggtt ccacataatc cagatggagc ctacaaccag
     2581 gatagcgatg attagcacgg tgaagacaaa cgccgtcatg ttccagcctt catctgattt
     2641 ggtattcatg tgcaggaagc acaccagatg caccagaacc tgtaccactg ccattgccag
     2701 gattgttccc agaattacgg ccggagaggc agctcctgtc atcaccatcc agaacggaat
     2761 caccgtcaga atgatcgaca ggataaagcc tgtcatgtag gtttttacgc tgccatggga
     2821 cgcgccgctg tgatcggtag aatgactcat tacatcgccc ccatcagata aacaacagtg
     2881 aacacacaga tccaaaccac atccaggaag tgccagaaca ggctcaggca catgatgcgg
     2941 gtacggttag tgctggtcag gccgcgacga gcgatttgta ccatcagcac cgccatccag
     3001 ataagaccgg aagtgacgtg cagaccgtgc gtgccgacca gcgcaaagaa cgctgacagg
     3061 aagccgctgc gatccggacc catgccgtta acaatcaggt gatggaattc gtagatttcc
     3121 atcccgataa atccggcacc aaacaaccag gtcaacgcca gccaggagat aacctggctt
     3181 ttgttgtttt tgtacatggc gatagccgcc atgccgtagg tgatggagct gaacaacagc
     3241 aagaaagttt caaccagaac gaacggcagt tcgaaaatgt ccttacctgt cgggccgcct
     3301 gcggtgccgt tcaccagaac ggcataggta gcaaacaaga ttgagaacag aatgcagtcg
     3361 ctcatcaggt agatccaaaa tccgaagatt ttggttccgc ctgcatcgtg gtgcccgtgt
     3421 tcgtgcgcgt gggcagtcgc gtgcgtcaaa gtatcagttg ccatttttca gccctgcctt
     3481 agtaatctca tcgaaatgct ggttttccag tttttcgatt tctgccaccg gcacgtagta
     3541 atccacgtcc tcgtcgaagc ttttcacgat ccaggtgatg atcatgcctg cgaagccaac
     3601 aatcgccagc caccagatat gccagatcat ggcgaaaccg aagatggtgg agaaagctgc
     3661 aatgacgata cctgcaccgc tgtttttcgg catatgaatt tcttcatagt ggtcaggctt
     3721 tttatacgct tcgcctttct ctttcatttc ccagaatgca tcacgctcgt gaacgtgcgg
     3781 cacaacggca aagttataga acggaggcgg ggaagaggtt gcccactcca gcgtacggcc
     3841 accccacggg tcgccagtca ggtcacggtt ctggtcgcgg tcgcgaatag aaacgtacat
     3901 ctgaataacg aggcagagaa tacccagcgc aatcagtact gcaccgctag ctgcaatcat
     3961 cagcatggtg tggaactgcg ggtcaatctg ctggctcaaa cgacgggtca tgcccatgaa
     4021 gcccagcgca tacagcggca taaaggcaac gaagaagccg atgatccaga accagaacgc
     4081 gcgtttaccc caggtttcat tcagtttgaa accgaacgct ttaggccacc agtaggtcat
     4141 ccctgcgaag cagccgaaga ccacgccgcc gatgataacg ttatggaagt gcgcaatcag
     4201 gaacaggctg ttatgcagaa cgaagtccgc gcccggtact gccagcagca cgccagtcat
     4261 cccgcccacc gagaaagtga cgataaaacc gatggtccac agcatcgcag aatggaacac
     4321 gatgcggccc tgatacatgg tgaacagcca gttgaagatc ttcaccccgg tcgggatggc
     4381 gataatcatt gtggtgatac caaagaaggc gtttacgttc gcgcccgcac ccatcgtaaa
     4441 gaagtggtgc agccaaacga tgaacgacag cacggtgata cagacggttg cccataccag
     4501 cgaggtataa ccaaacagac gtttacgcga gaaggttgcc gcaatttcgg agaacacacc
     4561 gaaaacaggc aggatcagga tgtaaacttc cgggtggccc caggcccaaa tcaggttgat
     4621 gtacatcatc atgttgccac ccatatcgtt ggtaaagaaa tgggtgccca gatagcgatc
     4681 cagggtcaac aacgcgacgg taaccgtcag aattgggaag gaagcaataa tcagtacgtt
     4741 cgcgcacagt gatgcccagg taaatactgg catcttgaac atggtcatgc ccggtgcgcg
     4801 catcttcaga atggtaacga agaagttgat accggtaagc gtcgtaccta taccggatag
     4861 ctggagactc catatccagt aatcgacacc gactcccgga ctgtactcta ttcccgatag
     4921 cggtggatag gccagccagc cggtctgcgc aaattcgccc acgccgagag aaacgttaac
     4981 cagaatcaca ccaacaacag taaaccagaa gcttaagttg ttgaggaacg ggaacgcaac
     5041 gtcacgcgcg ccgatctgca gcggaaccac caggttcatc agaccgataa cgaaaggcat
     5101 cgccacgaag aagatcataa tcacgccgtg cgcggtgaag atctgatcgt agtggtgagg
     5161 tggcaggaag cccgcttcgc ccgccgaggc aagagcctgc tggctacgca tcataatggc
     5221 gtcagcaaaa ccacgcagca acatcacaat cgccacgatg atatacatga taccgaggcg
     5281 tttatggtcg acggaggtca gccactcttt ccacaggtag gtccacttac cgaagtaagt
     5341 gatcaggcca acgagcgcca gacctcccaa aataatgcca gcgatcgtaa ccatgacgat
     5401 aggttcatgg aacgggactg catcaagtga taattttccg aacatcttta ttcttcctca
     5461 acccctttaa tgggcggatt ccgcgtggct catgtccatg ccttccatac cttcgtgtgc
     5521 actgtgctca ccttctggct gggtcatgtc catgctctta ccgtgagcca taaacttgtt
     5581 aatcacatcg gcaaacaagt ctggtttcac gttggagaaa tattccacct ggttgtattc
     5641 gctaggcgcg gccagttttt cgaacgcagc catgtcagac atggtgttcg gcgactgttt
     5701 cgcttttgcg acccattggt cgaattcggc gcgatccggt gttgcaatag ctttgaactt
     5761 catgcctgag aagcccggcc cgctgtagct ggcggagata ccgtcataag taccgggttc
     5821 gttggcgatc agatgcaggc gagtctgcat accggccatg gcataaatct ggctacccag
     5881 acgcggaatg aagaaggagt tcatcacgga gttggaggtc actttgaagt acaccggagt
     5941 gttcgccggg aaagcgattt cattcacggt tgcaatgccc tgttccgggt agatgaagaa
     6001 ccatttccag tccatggaaa ccacttcgat ggtaatgggc ttctcgtcgt gtgccagcgg
     6061 cttgctaggc tcaagagcgt gagtggtttt ccaggtcagt accgcaagga agatgatgat
     6121 taagataggt accgtccaga ccacagcttc cactttattg gagtgtgacc agttcgggct
     6181 gtacttagca tctttattgc tcgcacgata cttccaggcg aaaccaacag ccatcaagat
     6241 tgcgggaata acgacaatca acatcaggcc aaatgccgtc agtatcagtg aacgttgctc
     6301 cagaccaatc tgtcctttgg gatctaacag cgcagaatta cagccactga gcaatacagt
     6361 gcctgcaaat aatgacaacc atcccaaact tttattgtat ttcctgagtc tcatttaacg
     6421 acctcaattc cacgggatct ggtggcgttt aaagtgtgtg ggcattttac ggggaggtta
     6481 cattactgta aacatgatta aattcctgtt acttggattt ggctgtcagg ttacatatgt
     6541 tgcaaaacat atcaagttga ttttaagaag ctgttgcaaa agggcgttat aacgaaaggg
     6601 gagtgcagaa ggagtttgga cgggcaattg gtataaccaa tcttgaataa aacaattgtt
     6661 taacaaataa ttatcattat ggtgacaatt gggtgggggg aaaataaaga ctcattagct
     6721 gaatcgtgta agaaaaaata gaattcataa attgcgtcaa taatttccaa acgctaagcc
     6781 gcacaaaaga acaaatatta ttaagcgata ctgatgttta tttagccgta ataattacgg
     6841 cgagtgatta ctacagctaa ataatattta cagattacgt cagatgcgtt tttcgtagag
     6901 ccagataatc cagcaaacca ccaagcacga cgccagaaag ggcgactaaa accccgactt
     6961 ccagcagagc aggcaggaaa gagaagtgcg tcaaatccag cgcgtccatc gtcaacagca
     7021 gtaaccacac ggccaacagg ctgacgcccg ccgccagtgt ccacatggca aaggcataac
     7081 ctgtcggata ttcggtacgg gagataaagt tgtcatttac tcgtgtatat tcaagcgtct
     7141 ggcggcaaac cagcagcaaa ataagccccg gtacggcagc ggcgacggag aatagataga
     7201 atgtcgacca gccgtgtgct tcaacaaacc aacccgccac ggggccgaca taaactcgtc
     7261 ctacagcgga aagcgctgag agcagggcaa attgagtagc ggaaaatgac ttattacaca
     7321 gcgtcattaa cagtgcgaca aaggctgatg tgcccatccc gccacagagg ttttcgaaaa
     7381 agacggctgc gcccatgctg tagagatgct tatcagtaat cgacagcagc cagtaaccag
     7441 cgttagacgc accttgtaaa atgccgaaaa tcagcagcgc ccggaacagc gacaggcgct
     7501 gcatcaaaat cccaccgtac aatgcgccca caatggtcgc cagtaagcca agcgttttgt
     7561 taaccacgcc tacttcaccc gcatcaaacc cgacgccgcg aatcaaaaac gtggttgtca
     7621 ggctcatggc gaatgcgtcg cccagcttat acagcacgat aagaagcaaa ataagccagg
     7681 cattattgcg accaaagaaa tctcgcagag gtgcaacaac cgcttgttcc agcgtttttg
     7741 gcacaggaat ggtgtcggtc ggttctggtg caagcaacgt cgcgataata caagggatca
     7801 acagcgccgc catcaaccag tacatgccct gccagcccag ccatttatct gccagccaca
     7861 gggctaagcc gccggaaacc agcatcccta aacggtaacc cagcacgctg attgccgcgc
     7921 ccgcaccacg ttcttctgcc gggagcacat cggttttcca cgcatcgaag acaatatcct
     7981 gggaggccga gcaaaaagcg atcaccaccg ccagtgccgc catccagcgg agttgggtgc
     8041 caggttcgag aaaacccatc gccgcaatgg cgactaataa caggatttgc gtggcgagca
     8101 gccaaccgcg ccgccgccca aaaaatgggg gcgtgtagcg gtccatcagc ggtgaccaga
     8161 ggaatttaaa aacgtaagcc tggcctacca gagagaagaa accaatggtt ttgagatcaa
     8221 tattctcgac cgtcatccag gcctgtaagg taccggatgt cagggcgagc ggtagcccgg
     8281 aagcaaaacc caggatcagc aatatggctg aacgcggctg ttgaaaaata cgtaaatatt
     8341 gactggacat gggcgtctac aggcccggtt tgcaccgggc cagagggcag aaattaacgc
     8401 gcgttctgct tgatgaattc gtggatgctg gtgtcctgag acatatcagc gatggtatcg
     8461 gtcagcacac tgttaaccgc atcggcgata tttttgttgg aggcctggaa cgcaccttca
     8521 acgttgtagc tggcacgata gtttttggtc attttgttgc cattctgcgc ggtagcgatg
     8581 atggcgatat ccgctttggt cgcgatgttg tagcgcacgt tgccctggga cacgtcagca
     8641 tacagttggc taacgatgat ttgcagatta accgggccat tcggaccaac catgtaacca
     8701 cgcgcggtca tctgtttttc cagcacttct tgcagcagga aacgcagatc tcgggaggcg
     8761 gtcagggtaa cgatttgatt atcgcgggtg acttttgcca gcgcctgatc ggtacgctga
     8821 tcggcaccat taatgcttac ggtgacgccc atcaggcttg gatcctgctg tggcagtgta
     8881 atcgtcgggg aaacttcaat agttgttggc ggttttgcgc atcctgcaag cataaacaga
     8941 gcaactaacg ggaagaggat ttttttgaac atgttcgggc tctcagagac tcttaagcgt
     9001 gtttggtaaa aattcccgcc atcataacat tgccaacggc gaggggaagt gggtaaggca
     9061 tgtaaattca tcatgttggc gaaataatca cccctggtaa aagaaacgct gatgcgaggc
     9121 ctgtgtttca atctttaaat cagtaaactt catacgcttg acggaaaaac caggacgaaa
     9181 cctaaatatt tgttgttaag ctgcaatgga aacggtaaaa gcggctagta tttaaaggga
     9241 tggatgacat ctcagcgttg tcggaggaga tatttcatga tgatacgtga gcggatagaa
     9301 gaaaaattaa gggcggcgtt ccaacccgta ttcctcgaag tagtggatga aagctatcgt
     9361 cacaatgtcc cagccggctc tgaaagccat tttaaagttg tgctggtcag cgatcgtttt
     9421 acgggtgaac gttttctgaa tcgtcatcga atgatttaca gtactttagc ggaggaactc
     9481 tctactaccg ttcatgcgct ggctctgcat acttacacta ttaaggagtg ggaagggttg
     9541 caggacaccg tctttgcctc tcctccctgt cgtggagcag gaagcatcgc gtaaaaacgc
     9601 atttgcaact gtcggcgctt ttccagtatg ttgctaaaga ttttat
//
LOCUS       DAZOYL010000143         9619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZOYL010000143 DAZOYL010000000
VERSION     DAZOYL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.143"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..203)
                     /locus_tag="ACHUOY_002542"
     CDS             complement(<1..203)
                     /locus_tag="ACHUOY_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR0683295.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPAGNDSNLLIVFYVQI"
     gene            complement(203..1129)
                     /gene="glsB"
                     /locus_tag="ACHUOY_002543"
     CDS             complement(203..1129)
                     /gene="glsB"
                     /locus_tag="ACHUOY_002543"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR0683296.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1193..2581)
                     /gene="sad"
                     /locus_tag="ACHUOY_002544"
     CDS             complement(1193..2581)
                     /gene="sad"
                     /locus_tag="ACHUOY_002544"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR0683297.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2682..3563
                     /locus_tag="ACHUOY_002545"
     CDS             2682..3563
                     /locus_tag="ACHUOY_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0683298.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3640..4755
                     /gene="yneK"
                     /locus_tag="ACHUOY_002546"
     CDS             3640..4755
                     /gene="yneK"
                     /locus_tag="ACHUOY_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0683299.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4905..6095
                     /gene="ydeA"
                     /locus_tag="ACHUOY_002547"
     CDS             4905..6095
                     /gene="ydeA"
                     /locus_tag="ACHUOY_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR0683300.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6120..6785)
                     /gene="marC"
                     /locus_tag="ACHUOY_002548"
     CDS             complement(6120..6785)
                     /gene="marC"
                     /locus_tag="ACHUOY_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR0683301.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6997..7431
                     /gene="marR"
                     /locus_tag="ACHUOY_002549"
     CDS             6997..7431
                     /gene="marR"
                     /locus_tag="ACHUOY_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR0683302.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7451..7834
                     /gene="marA"
                     /locus_tag="ACHUOY_002550"
     CDS             7451..7834
                     /gene="marA"
                     /locus_tag="ACHUOY_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR0683303.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7866..8084
                     /gene="marB"
                     /locus_tag="ACHUOY_002551"
     CDS             7866..8084
                     /gene="marB"
                     /locus_tag="ACHUOY_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR0683304.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(8141..9580)
                     /locus_tag="ACHUOY_002552"
     CDS             complement(8141..9580)
                     /locus_tag="ACHUOY_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0683305.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2485 a         2465 c         2167 g         2502 t
ORIGIN      
        1 attatctgaa cataaaacac tatcaataag ttggagtcat tacctgccgg agcaagacgt
       61 ttagcgacaa aatccgccgc atgttgttcg atgactggcg cgcctttctc caggcaatac
      121 tgacgctctt tcatgcccag acgaaagcga gagcgaaatt ttgaacggga taaacgcgcg
      181 aagagtggat cgagagactg cattaataaa ccgaacgtcc taactgtttc gtcaattgtt
      241 caaggacggc gatacccgca agcgagttac ctgcatcatc cagttccgga ctccagactg
      301 cgatggccat ttcatgcgga acaatcgcca caataccgcc accaacgcca gatttcgccg
      361 gtagtcccac ccgccaggca aactcccccg cgttctggta cataccactg gtcgccatca
      421 gcgcgttaat ttgccgcgcc tgcattggcg tcactacagg ttcatcaata tgaatagctt
      481 tcccctgatt agccagaaag acaaacgtcc gagccagctc tacacagctc attttcagag
      541 cgcagtaatg aaagtagttt tgcagaacgg ttgtcacgtc atgatggaaa ttgccaaacg
      601 acttcatcag ccaggcgata gccgcatttc gcgcggaatg ttcaaattcg gaacgcgcta
      661 ccaccgtatc gtatgaaata tcagacacac cgcttaagcc gcgcacgact tccagcatac
      721 gttgccgtgg tgcgcttaat cgcccttgca acatatcgca gaccaccagc gcaccagcat
      781 taataaacgg attacgcgga ataccctgtt ccatttccag ttgcactaaa gaattgaacg
      841 gtgatccaga cggatctttg ccgacgcgtt gccagatttc ctcttcggag taatgacgca
      901 tggcgacaac gagactcagc actttggaaa tagactgaat ggaaaaacgt tcttgcgcgt
      961 ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc aatgcccaat cgggaaccgt
     1021 ctactgtagc cagcgccgga atataatccg cgactttacc ctgaccaatg agcggccgca
     1081 cttgccgcaa gatgttttct aaaattgcat tatccatggc gactgccact ttctactcct
     1141 ggaccgcagg tctgaaaaga cctgcgagta tatcagagct gaatatgtcg cgtcagatcc
     1201 ggtctttcca caccgtctgg atattacaga attcgtgtaa gccgaaatgg gaaagctcac
     1261 gaccaaagcc actctttttc acgccaccaa aggccactcg cgcgtcgctg gcacaataac
     1321 cattgataaa caccccaccg cattccagac gtgccgccat ctgtctggcc tgtgtttcgt
     1381 cggtggtaaa aatggtcgct gaaaggccga actcactatc attagccagt tccagcgcat
     1441 gttctgcatc tttcgcaacg gtgattgccg caacggggcc aaacatttct tcccgaaacg
     1501 cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg atagtagtta cctgccccag
     1561 ccatcttttc cccgcccagt aacaaacgcg caccctgcgc cagggttttc tctacctgat
     1621 gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc gagagtgttc tcttcgtcac
     1681 ggggatcgcc cattttcaag gctgccgcag ctgccacaaa acgttcggta aatgcagaag
     1741 caattccctc ttcgataata aagcgttttg ccgctgcaca tacctgtccg gtattctgat
     1801 aacgtccggc taccgccgct ttaaccgcca gttccagatc ggcatcgtta agcacaataa
     1861 acggatccga accgcccagt tccagtacgc attttttcag tgccgctcca gcctgtgcgc
     1921 caatagccgc tccagcacga acacttccgg tcaccgtgac agcagcaatg cgcgagtctt
     1981 taatcatctg actgacaccg tcgttgtcgg cattcagcca gccatatacg ccttgcggga
     2041 ttcccgcatc tttaaacacc tgggcaatga gctgagcaca tcccatcaca ttcggcgcat
     2101 gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac agcgccacgc atcacctgcc
     2161 ataacggaaa attccacggc ataatcgcca gaatcgtccc caacggtcga tactcaataa
     2221 ccgcctgctg attttccacc agtgtaggtt ccgccttcag cattgccgga ccatgttctg
     2281 cataccagtc acacaaattc gccgatttcg ccacttcagc gcgcgcctgg ttgattggtt
     2341 tgcccatttc gcgggtgatc atttgcgcca tttcttcgct acgggcgcgc agagccttac
     2401 cgatatcacg cattttttca gcacgataat ctatatttgt ctcgcgccag tcgcgaaagc
     2461 ctgctgccgc cagctcaagt gcgttttcga tatcgttagc gccagcccac ggcagcacag
     2521 aaagttgttc acccgtggca ggatttattg aaattgcatg agttgccgga gtaatggtca
     2581 tcggggtatc tcctttatga gtcatggtat gaagatacgc agatttactc ttgctttaaa
     2641 atgaataata ttaagccact tattcacgaa tcgagaatgt tatggatctg acccaactgg
     2701 agatgttcaa cgcggttgcc gaagctggca gcattaccca ggctgcagca aaagtgcatc
     2761 gcgtaccgtc gaatctcact acccgtctac gccagctgga aacagaactg ggggtagatc
     2821 tgtttattcg cgagaatcag cgtttacgtc tctctcctgc cgggcataac tttttacgct
     2881 atagccaaca aattctcgcg ttagtggatg aagcgcggag cgttgtcgct ggcgatgagc
     2941 cgcaaggttt attttctctt ggttcgctgg aaagcaccgc agcagtgcgc attccggcca
     3001 cgctggcgga attcaaccat cgttatccca aaattcagtt ttcactttcc accggccctt
     3061 ccggcactat gctggaagga gtgctggagg gaaaactgaa tgcggcattt attgatgggc
     3121 ccattaacca tactgccatt gacgggatac cggtataccg cgaggagctg atgatcgtca
     3181 cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt taacggcagt aacatttatg
     3241 ccttccgcac caattgttcg tatcgtcgcc acttcgagag ctggtttcat gctgacggtg
     3301 ccgctccggg aactattcac gagatggagt cgtatcacgg gatgttggcc tgtgtggtcg
     3361 caggagcagg cattgcgctt attccacgct ctatgctgga aagtatgccg gggcatcacc
     3421 aggttgaagc gtggccgtta gctgagcaat ggcgttggtt aacaacctgg ctggtctggc
     3481 gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca actactggat gtgtctgact
     3541 cggcaaaaca gagttaccaa tgaactattt tgataattct tgcggctaat atgctctata
     3601 tagtgatgtt ccgatgactt atgactatat ggggcaaata tggttacgcc agtaagcatc
     3661 agcaattaca tatctcttcc tgatgatttt cccgttcgta atattgcacc acaggtaaaa
     3721 gaagttttaa aggactttat tgatgcgcta agtacaataa tatgtgatga agagtggcgc
     3781 acgtctttaa acatcaactc agccacaaaa aagatattta ataaccttga taacttatca
     3841 tatattcaga ggacttcttt ccggggtaac gacacgctat acaatgaaaa ggttcagttt
     3901 aaactcactt atcccgtcaa gaatggaaga cacaaagaaa atattgaatt tcaggtagta
     3961 ataaatttaa gccctattta tttagataat tttcgccatg atggagaaat taatattttt
     4021 tgcgctccca acccaaagcc tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt
     4081 gtgctctttc tgtttctgaa tgattttatt gaacaatttc caatgttcaa ccctggcgtt
     4141 ccgatcaaaa tagcgcatac accacatatt gaacccctgc ctccggatca ccataccgct
     4201 gcagattact tacgccagtt tgatttgctt gtcctgaatt ttatctctcg cggtaatttt
     4261 gtcatactcc cccgattatg gaataactct gaggttcaca gatggttcgt caataaagat
     4321 cctaacttga tcaccgccat tctcgacata acggacagcg aattaaaaga ggatttattg
     4381 caaagcctga tggattcatt aggttctaac aaacatgtac tacccgaagt ctgcatctgc
     4441 tttttattcc ttttagcaga acaagaatct cctcattttc aggacttgtt tttatttttc
     4501 gccaatatgt tattgcacta tcaccaattt atgaatccca atgaaagtga tttgaatgac
     4561 gtgttgatgc cagcatcatt aagtgatgat aaaattatca agcatatggc gcgcaggacc
     4621 ctcgaactgt ttgtaaaaaa tgaaacaccg ccaaaagtta ctcacgaaga cctggtgaaa
     4681 aacaggcctc gctcccctgt cagaccacct atacccgcaa cctccaaaac gccagacctc
     4741 cctgaacgtc attaaaccgt gatgttaccg actctctgac gcggaacaga atcagcgtca
     4801 gagaaacgga aaacgcgatc cagatcacaa atacattgta ttcacatcat taaccgtttt
     4861 aagatcattt catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg
     4921 tttcccgcaa agtggcgtgg ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca
     4981 acaccaccga atttgtccct gttggcctgc tctctgacat tgcgcaaagt tttcacatgc
     5041 aaaccgctca ggtcggcatc atgttgacca tttacgcatg ggtagtagcg ctaatgtcat
     5101 tgccttttat gttaatgacc agtcaggttg aacggcgcaa attactgatc tgcctgtttg
     5161 tggtgtttat tgccagccac gtactgtcgt ttttatcgtg gagctttacc gttctggtga
     5221 tcagtcgcat tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtctctgg
     5281 cgatccgtat ggctccggcc gggaagcggg cacaggcctt gagtttaatt gccaccggta
     5341 cagcactggc gatggtctta ggtttacctc tcgggcgcat tgtggggcag tatttcggtt
     5401 ggcgaatgac cttcttcgcg attggtattg gggcgcttat cacccttttg tgcctgatta
     5461 agttacttcc cttactgccc agtgagcatt ccggttcatt gaaaagcctc ccgctattgt
     5521 tccgccgccc ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt
     5581 acacggcata cagctatatc gagccttttg tgcaaaacat tgcgggattc agcgccaact
     5641 ttgccacggc attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg
     5701 gtaaactggg taatcagtat gcgtctgcgt tggtgagtac ggcgattgcg ctgttgctgg
     5761 tgtgtctggc actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta
     5821 ttttctgggg gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc
     5881 tggcaccaga tgctaccgac gtcgcgatgg cgctattctc cggcatattt aatattggaa
     5941 tcggggcggg tgcgttggta ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg
     6001 gttatgtggg cgcggtgcct gcttttgccg cgttaatatg gtcaatcatt atatttcgcc
     6061 gctggccagt gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt
     6121 taatggtacg ttttaatgat ttccaggatg ccgttaataa taaactgcac acccatacat
     6181 accagcagga atcccatcag acgggagatc gcttcaatgc cacccttgcc caccagccgc
     6241 ataattgcgc cggagctgcg taggcttccc cacaaaataa ccgccaccag gaaaaagatc
     6301 agcggcggcg caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggac
     6361 gccgagctaa taatcatcgc tatggttccc ggaccagcgg tacttggcat tgctagcggc
     6421 acaaaggcaa tattggcact gggttcatct tccagctctt ccgacttgct tttcgcctcc
     6481 ggtgaatcaa tcgctttctg ttgcggaaag agcatccgaa aaccgataaa cgcgacgatt
     6541 aagccgcctg caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc
     6601 ccggcgtaat acgccaccat catgatggca aatacgtaca ccgaggccat caacgactga
     6661 cgattacgtt cggcactgtt catgttgcct gccaggccaa gaaatagcgc gacagttgtt
     6721 aatgggttag ctaacggcag caacaccacc agccccaggc caattgcttt aaacaaatct
     6781 aacattggtg gttgttatcc tgtgtatctg ggttatcagc gaaaagtata aggggtaaac
     6841 aaggataaag tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat
     6901 ttagcaaaac gtggcatcgg tcaattcatt catttgactt atacttgcct gggcaatatt
     6961 atcctctgca actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc
     7021 aatgaaatta ttccattggg tcgcttaatc catatggtta atcagaagaa agatcgtctg
     7081 cttaacgagt atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct
     7141 atccgctgcg cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg
     7201 ggagcactga cccgtatgct ggatcgcctg gtctgtaaag gctgggttga aaggttgccg
     7261 aacccgaatg ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgc
     7321 gaacaatgcc atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg
     7381 gcggacgaag ttgcaacact tgagcatttg cttaagaaag tcctgccgta aacaaaaaag
     7441 aggtatgacg atgtccagac gcaatactga cgctattacc attcatagca ttttggactg
     7501 gatcgaggac aacctggaat cgccactgtc actggagaaa gtgtcagagc gttcgggtta
     7561 ctccaaatgg cacctgcaac ggatgtttaa aaaagaaacc ggtcattcat taggccaata
     7621 catccgcagc cgtaagatga cggaaatcgc gcaaaagctg aaggaaagta acgagccgat
     7681 actctatctg gcagaacgat atggcttcga gtcgcaacaa actctgaccc gaaccttcaa
     7741 aaattacttt gatgttccgc cgcataaata ccggatgacc aatatgcagg gcgaatcgcg
     7801 ctttttacat ccattaaatc attacaatag ctagttgata acgtgacaac gtcactgagg
     7861 caatcatgaa accactttca tccgcaatag cagctgcgct tattctcttt tccgcgcagg
     7921 gcgttgcgga acaaaccacg cagccagttg ttacttcttg tgccaatgtc gtggttgttc
     7981 ccccatcgca ggaacaacca ccgtttgatt taaatcacat gggtactggc agtgataagt
     8041 cggatgcgct cggcgtgccc tattataatc aacacgctat gtagttattg tcaggctctg
     8101 atcacagagc ctgacatctc cgtaatatct tcctcaagtc tcaaatattt tcgccattgt
     8161 tggcgatgag atcccgatac cagtaaaacg acgctttacg tatccgttcc agcgttccct
     8221 tcccttcgtt gtctttatcg acataaatca ttccgtagcg ctttttcatt tcccctgttc
     8281 cggcagaaac caggtcaatg cagccccacg gggtgtagcc aattaagtca acaccatctt
     8341 caactacggc ttttttcatt tcccgaatat gggaagcaaa gtaatcaatg cgatagtgat
     8401 cgttcaccgt gccgtcagct tgtctctggt caaccgcacc aaatccattt tcgacaataa
     8461 acagcggcaa ctggaaatga tcccagaacc agttgagtga ataacgtagc cctgccggat
     8521 caatttgcca tccccattcg gatgtatcga tataagggtt gctgaccaaa tctcgtggtt
     8581 caacataatc cagttgcggg ttatcgggag aaaattgtgt cgtaaaagac atgtagtagc
     8641 taaaaccgat aaagtcgaca catccactgg caagaatcgc gttatcttct ggtgtgatat
     8701 cgaggttgaa tcctttcctg gcaaagtaat tcagcatatg ttgcggataa tatccacgag
     8761 catgaacatc agtaaaccag taacgacgat gcatcgcttt cgtggccatc atcatatcgt
     8821 tgggtgcaca cgtcagagga tagatggggc acatggcgat catacagcca atgttaaatt
     8881 caggattgat cgattttcca gtctgtaccg ccagggcact ggcaactaac tcgtaatgta
     8941 ccgcctggta cattatttgt tcgcgctcat tgatatcttc ctctggcgaa tacaagatac
     9001 cggaattagt aaatggacac aggctttcgc tgaaattcac ctgattattg atttcgttaa
     9061 acgtcatcca gtactttact ttttctttat agcgcgtgaa gaccgttgat gcgaagcgga
     9121 taaaaaagtc gatcagctta cggtttcgcc agccaccata ttttgtcacc agatgataag
     9181 gcatctcaaa atgcgaaagc gtcaccacag gttccattcc ttgcttcagg cattcatcga
     9241 acagatcatc ataaaattgt aaaccctctt cattcggctc ctgttcgtca ccttgcggaa
     9301 agattcgtgt ccaggcaatg gaagttcgaa agcatttgaa tcccatctcg gcaaataact
     9361 gaatatctgt tttatagcga tgataaaaat caattgcttc atgattggga taattaagcc
     9421 cgtcgataac gccttctgtc acttcacgcg gcaccccgtg agcgccagca gtcatcacat
     9481 cagcgatact gatgcctttt cctccttcat tccagccacc ttccaactga tgcgcggcta
     9541 ccgcgccacc ccataaaaaa ccttttttaa atcctgacat acacaactcc ttaactaaaa
     9601 tagattacca ccagacttc
//
LOCUS       DAZOYL010000144         9566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZOYL010000144 DAZOYL010000000
VERSION     DAZOYL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.144"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(10..2190)
                     /gene="katG"
                     /locus_tag="ACHUOY_002553"
     CDS             complement(10..2190)
                     /gene="katG"
                     /locus_tag="ACHUOY_002553"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR0683306.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(2519..3409)
                     /gene="metF"
                     /locus_tag="ACHUOY_002554"
     CDS             complement(2519..3409)
                     /gene="metF"
                     /locus_tag="ACHUOY_002554"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR0683307.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            3485..3619
                     /locus_tag="ACHUOY_002555"
     CDS             3485..3619
                     /locus_tag="ACHUOY_002555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683308.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(3758..6190)
                     /gene="metL"
                     /locus_tag="ACHUOY_002556"
     CDS             complement(3758..6190)
                     /gene="metL"
                     /locus_tag="ACHUOY_002556"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR0683309.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6193..7353)
                     /gene="metB"
                     /locus_tag="ACHUOY_002557"
     CDS             complement(6193..7353)
                     /gene="metB"
                     /locus_tag="ACHUOY_002557"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR0683310.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            7630..7947
                     /gene="metJ"
                     /locus_tag="ACHUOY_002558"
     CDS             7630..7947
                     /gene="metJ"
                     /locus_tag="ACHUOY_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR0683311.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            8131..8739
                     /locus_tag="ACHUOY_002559"
     CDS             8131..8739
                     /locus_tag="ACHUOY_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR0683312.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            8884..9566
                     /locus_tag="ACHUOY_002560"
                     /pseudo
     CDS             8884..9566
                     /locus_tag="ACHUOY_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2297 a         2664 c         2428 g         2177 t
ORIGIN      
        1 aaggtcagat tacagcaggt cgaaacggtc gaggttcatc actttcaccc atgccgccac
       61 gaagtcttta acaaacttct cgtgagcatc gctgctggcg taaacttcag ccaccgcacg
      121 caggacagag ttagaaccaa ataccagatc cgcacggctg gcagtgtatt tcacttcgcc
      181 ggtttcgcgg tcacggcctt caaacagctc tttcgattcg tcggtcgctt tccactcgta
      241 acgcatatcc agcaagttca cgaagaagtc attgctcaat acgccaacgc ggtcagtgaa
      301 gacgccgttt ttgctgccat cgaagttggc acccagtacg cgcatcccgc ccaccagcgc
      361 ggtcatttcc ggcgcggtca gggtcagttg ctgggctttg tcgatcagca gtgactcggt
      421 ggtggaaaca tccagacgag cgcgatagtt acggaaaccg tcagcaattg gctccagcag
      481 ctcgaacatc tcaatgtcag tctgatcctg acgcgcatca acgcggcccg gcgcaaacgg
      541 tacatgaatg ctcaaacctg cggcgcttgc ggctttctca acaccaacca caccagccag
      601 cacgatgata tccgccagcg aggctttacc agactctttc tggattttct ccagaacagg
      661 cagagcacga acggctgcgg cgttcacatc ccagtcgcgc tgcggcatta atgccagacg
      721 cgcaccgttg gcaccaccgc gtttgtcgcc accacggaag gtagaagcag atgcccaggc
      781 caccgatacc cgctcactaa cagacagacc agaatccgca atcgcgaatt tcagatcgat
      841 aatgtcctgc tcggtcgggt tgtagatcgg ctgcggcagc ggatcttgcc agatcagatc
      901 ttctttcggc acttccggcc cgatgtagcg agatttcggc cccatatccc tgtgcgtcag
      961 tttgaaccag gcacgggcaa aggcttcgtt gaacgcctgc ggatcgttga ggaaacgacg
     1021 agagatcttc tcgaactcag gatcaaaacg cagcgtcagg tcggtcacca gcatcgtcgg
     1081 tttacgtttc ttcgacggat cgaatggatc cgggataatt tccggtgcgt ctaccgcttc
     1141 gaactggatt gcgccagccg gactgcgggt ctgtacccac tcatatttga acaggttttc
     1201 gaagaaatag ttgctccact gggtcggcgt ctgggtccag acaacttcca gaccagaggt
     1261 aatggcatct gcgccaacgc cgctgccgta agtgctcgcc cagcctaaac cttgttcttc
     1321 aatcggtgca gcttctggat caggacctac gtttgatgtc ggaccggcac cgtgggtttt
     1381 acccagcgta tgaccacccg caatcagcgc cacggtttct tcgtcgttca tgcccatgtt
     1441 gccgaaggtc gcgcggatag ctgctgccgc agaaagcggt tcgccgctgt gatccgggcc
     1501 ttccgggtta acgtaaatca ggcccatctc ggttgcaccc agcggtgctt tcgccagcgc
     1561 ttccggatga cggtgagtca gccaggcttt ttcatcaccc cagttaacat ccagatccgg
     1621 ttcccagacg tcttcacgac cggcaccaaa accgaaggta cggaagccgg agttttccag
     1681 cgccacgtta cctgcgagga taaacaggtc ggcccaggag attttctgac catatttctg
     1741 tttgattggc cacaacaggc gacgcgcttt atcgaggctt acgttatccg gccaggagtt
     1801 cagcggtgca aaacgttgct gaccacgacc cgcgccgccg cgtccatcga ttgaacggta
     1861 agtacctgcg ccgtgccagg ccatacgaat aaacagaccg gcgtagctgc cccagtcggc
     1921 tggccaccac ggctgagatt ctgtcagcag ggctttcaga tcttttttca ggccgtagta
     1981 atctaatttg ctgaattctt tgcggtagtc aaagtcctca cccagcgggt tagaacgatt
     2041 agaatgttgg tttaacaggt caacacggag ttgatttggc caccagtcgc gagtggttgt
     2101 gcccgccccc gcgctctggt cgtgaccgcc ctgatggaac gggcatttgc cagtggctgt
     2161 cgtgttatgg atatcgtctg acgtgctcat caatgtgctc ccctctacag tgttaccgtt
     2221 acgatacaca gcgttagaga gaagttataa ttgaattaat ccacggatgc gatagttgag
     2281 atcttacata ttgttggtta aagagatgta ggtcaaattg atcgtaatta agcttttttg
     2341 attatgtccg aattcggaca aatgctttat aaaaaagggt tttagcttca tatccttcaa
     2401 gtaatgaggg agaaaacaag gctgacaacc ttattttccc gccctcattt cgaggcagca
     2461 ttttgtgctc tgtttaaaat ttgtgatcac tgtgtgattt tcacaaaagc cacactattt
     2521 ataaaccagg tcgaacaccc agcgtatggc aaatcgcgta actcatttca gcgcggttaa
     2581 gcgtatagaa gtggaaatct ttcactcctt cacggcttaa aatcttcacc atatccatgg
     2641 caatattcgc gcccaccagt ttgcgggttt cggcatcatc atccaggccg tcgaacattt
     2701 gcgccatcca cgccggaata cgcacgttgg tcatatcggc aaatttcttc gcctgtttaa
     2761 agttagatac cggcaaaatt cccggaataa tttccacatc aatgcccgcc gatacacagc
     2821 ggtcacgaaa acgcaggtag ctttcgacat cgaagaagaa ctgagtaatc gcgcggttgg
     2881 ctccggcatc gactttgcgt ttcagattaa gcaaatccgc ctgagcgctt tttgcttccg
     2941 ggtgaacttc cggatacgcc gccacggaga tatcgaaatc tgccacttct tttaatagcg
     3001 tcaccaggtc agaagcatac atttctggct taccacttcc cggcggcaga tcgccacgca
     3061 gcgccacgat atgacgaata ccgttattcc agtagtcgcg tgcaatggtg cgcaactcgt
     3121 cgggcgtcgc atcaatgcaa gtaagatgcg gtgccgcttc cagaccagtg cgatctttaa
     3181 tgcctttaat aatgctgtgc gtacggtcgc gctcgccgga gttcgcgcca taggtcaccg
     3241 atacaaactt cggtttcagg ctgctaaggc gatcgatgga gttccacagg gtctgctcca
     3301 tttcactggt acgcggcggg aaaaactcga acgaaacgtt aatctgcccc tggacttctg
     3361 ccaggctctg attcagggca tcccgctggc tggcgtgaaa aaagctcata ccttacctca
     3421 tcaatcgctt gtagttatat gttgtgttgt gcacatctat ccgtttagac gtccagatgt
     3481 aaagatgaag gaaaagccga agggcgtcaa ccgaaaaatg accaatgatg gtgaggaaaa
     3541 atcagggaag atgaaaaaac ttcaggatgg taagaaaaag aaaatccggt atgcaaagca
     3601 aaccggactg caaatataaa ctaaccatgt acttattgct tcttgccgga ttcgtcgtaa
     3661 acgcttaatc cggcctaatc ctatgataat tcaatatatt tcattagaaa ccataaaccc
     3721 gaaaacatga gtaccgggca ttattaaatt tctgaaatta caacaactgt gccagccggt
     3781 tgatatccga ctgaatcgcc ccggcggtga cgtcgcgccc agcgccaggc ccacggatca
     3841 ccagcggatt atcgcgatac cagcggcttt cgatggcaaa gacgttatcg cacggcagca
     3901 gtgatgccag cggatggtct tcacgcaccg cttccacgcc cacacgcgct ttgccattgg
     3961 catcgaaacg cgccacgtag cgcagcacca gccccatttc gcgggccgct tccagccgtt
     4021 gcaccatctg ctcgttaagt tcatcgccat tttcaaagaa atggtcgatg ctgccgcctt
     4081 cgcaatgggc aggcaccagc gactccacgc gtacctgatc cggttcgatg ttgtaacctg
     4141 cttcacgcgc cagaatcacc agcttgcgca tcacgtcttt gccagagaga tcgtcacgcg
     4201 ggtcaggttc ggttaagccc tgctgccacg cctgatccac cagctcggta aacggcacgc
     4261 taccgtcgaa ttgcaggaac aaccaggaga gcgtgccgga gaagatcccg ctgatcgaca
     4321 aaatagtatc gccgctgtcg atcagatcgc gcacggtgtg gttgatcggt aagcccgcac
     4381 cgacggtggc attgtacagc cagtgacgcc cggttttttc gaaggcgtcg tggatctggc
     4441 gatatttatt gctgtcgctg gctcccgcca gtttgttggc gctgatgacg tggaaaccgt
     4501 ggctggcgaa atcaagatac tgatcagcaa gctgctggct ggcggtaacg tccagcacca
     4561 ctaaatcatc atacggatgg gcgcgcatcc acaggaacaa cgactcttca tcctgctcaa
     4621 ccgcttcatc gttgaagaag gctaacgcgc ggctggcgtc cagcccgtca tagctcaaca
     4681 gactgcggcg gctgtccacc acgcctgcca gcacaaattc aaagtcggta cgtgccgaaa
     4741 gcgtgctctg ctcacgggcg aacagttcca gccaacggga accgatattg cccttaccga
     4801 acaataccag gccgatgcgt ttttctgcgc ggaagacgga ctgatgcagc ccctgaatca
     4861 ggctttcggt cgggccggtg cgcagtactg ccaccaggct gatgccgtca tcggactgcc
     4921 aggtaaattc gaccggctgg cctttcagtt gctgccagaa gcggtggcaa tgcagcgggt
     4981 tacgggtgac gcctgcaccg accatcgcca ccagcgccag cccctgacgc aggcgcagtt
     5041 cgccaggtaa ccccgcttcg tcgaggattt tcagcgcact gtcggccact tctgaggtgt
     5101 agcaaaattg cagcaactgg cgatcgttat gtacgccaac cgccagcggg cgtacctgcg
     5161 cgcgtttcag gatttggtcg atctctttat gcgccagttt gaaatcctga ctggcgggca
     5221 cctgaaactc aatcaaacag acatcatcgt ggctggtgac aatacgcgca ccagtaccgg
     5281 aggccagcac gcgttcaatg cgcgtggaac cttgatccgg cgtgtagcta cagcgcagtt
     5341 gcaggtcgat ttcgctgcca gaaaccggct gtaaagtacg ggcgtgaaga acgggagccg
     5401 ccagacgagc cagttcgctg gcttcatcca gacgcagcaa cggcagcaga caggcatctt
     5461 tcactttacg cgggtcggca ctgtataccc cggcgacgtc gctccagatg gttacacgag
     5521 aaacacccgc cagcgcaccg atttgtgtcg cggaatagtc ggaaccgtta cgccccagca
     5581 gcaccgtttc accggcgttg ttacggctga taaacccggt caccaccaga cgtttgcccg
     5641 gatgttgcac cagtagctgt tgcagcaacg ggtaagaaag cccttcatca acctgcggtt
     5701 gtgcggcgcg ttcagcgcgt aaaaactcgc gggcatcaag ccaggccgct ggcagccctt
     5761 gttgattaag taccgcagac atcaggcgtg ccgaccatac ttccccgtgg cccaccactt
     5821 ccgcatacac tgcgtcgtta ataccgctgt cgagcagcgc cgccaggcgt tccagatcgc
     5881 tgacaaaagc gctaatgagg ctatcggctt cttcagcggg tagcagaccg ctaatcagat
     5941 cgcactgata gcgacgcagc gtttgttgaa cctgatgcgc agagagacga tcggtctggc
     6001 ttagtttcaa ccagttaatc aactggttag tggtgctacc ggcggcggaa accaccatca
     6061 tatcgtcagg ctgagagtac tccgccataa tgcccgcgac acgcaaataa cacttcacat
     6121 cagccagact actgccacca aatttatgca gctgacgacc tttcgcccct gcctgcgcaa
     6181 tcacactcat ttttacccct tgtttgcagc ccggaagcca ttttccaggt cggcaattaa
     6241 atcttcgcca tcttcaatac cggtggagat acgcagcagc gtctcggaga tcccggcggc
     6301 agcacgcgct tctggtgcca tgcctgcatg tgtcatggtt gcggcgtgag agattaaact
     6361 ttccactccc cctaatgatt ccgccagcgt aaacaacgac agcccgctca ggaaacgacg
     6421 cagcgtctgc tcatcgccat ccagttcaaa actcaacatt gcgccaaagc ctttttgctg
     6481 acgcgcggca atttcatgcc cctgattttc cggcagcgac gggtgataca gttttttcac
     6541 caacggctgg gtttgcagat atttcacaat cgcctgcgcg ttgcgctgcg ccagctccat
     6601 acgcggcacc agcgttcgca acccgcgtag cagcagatag ctgtcaaacg cgccgcccgt
     6661 cacgccaata ttgtttgccc accaggcaag ttcagtgaca acgtccgggt ctttagcaat
     6721 caccacgcca gccaccacgt cagagtgacc gttcagatat ttcgtgcatg aatgcaacac
     6781 cagatcggca cctaatgcca gcggattttg taatgccggg cttaagaagg tgttatccac
     6841 cacgctcacc gccccgactt cccttgccag atggcagatt ttcgcaatat ccacgacgcg
     6901 taacaatgga ttacttgggc tttctaccag taccagtttg ggtttttctg ccagcgctgc
     6961 ccgtaatgcc tgttcatcgc cttgatcaac aaacaacacg cgatagcaac cgcgtttcgc
     7021 cagactgtcg aacaggcgat agctaccgcc gtagcagtcg tgcggcgcaa ccagcagatc
     7081 gccaggtttc aaaaagacgg tcgttaccag gtgaatcgcg gacatgccgg tattagtaag
     7141 tactgcgcca gcaccgcctt ccagttctgc cagcgcacgc tgaaccacat cgcgcgttgg
     7201 gttgccgcga cgcgagtaat catgcgcgcg cggttcatta aatccggtaa agttataggt
     7261 gctggaaaga tggatgggag ggacaacgca accatactgt tcgtcgtcat ttaacccgct
     7321 acgcactgcg atggtggcct gtttacgcgt catgtgatga agtttcctgg gctttgtcgg
     7381 tgaaatgtca ggcaccagag taaacattgt gttaatggac gtcaatacat ctggacatct
     7441 aaacttcttt gcgtatagat tgagcaaatc ccaaatagcc gttaaaatta tatgcattat
     7501 cacgccgaca ggtgcattac acgatgtcac ggtaacgcct gtacggtaaa ctatgcgggt
     7561 ttacggtcag tacccacata aattgtgtgg tctggtctca atttattgac gaagaggatt
     7621 aagtatctca tggctgaatg gagcggcgaa tatatcagcc catacgctga gcacggcaag
     7681 aagagtgaac aagtcaaaaa gattacggtt tccattcctc ttaaggtgtt aaaaatcctc
     7741 accgatgaac gcacgcgtcg tcaggtgaac aacctgcgtc acgctaccaa cagcgagctg
     7801 ctgtgcgaag cgtttctgca tgcctttacc gggcaacctt tgccggatga tgccgatctg
     7861 cgtaaagagc gcagcgacga aatcccggaa gcggcaaaag agatcatgcg tgagatgggg
     7921 attaacccgg agacgtggga atactaaagc aaaaaagagc ggcgcggagt ggaatcgcct
     7981 gatgcgctac gcttatcagg cctacgtcat attgcaattt attgaatttg cacgaacttg
     8041 taggccggat aaggcgttca cgccgcatcc ggcataaaca acgagcacgt tgtctgcgac
     8101 ccaccgcttt ttatacatgg acgtttaact atgaaaaaca ggctgctgat cctcagcctg
     8161 ctggtttctg tacctgcctt tgcctggcag ccacaaaccg gcgacatcat ctttcagatc
     8221 tctcgctcat cgcaaagtaa agcgatccaa ctggcgaccc attccgatta tagccacacc
     8281 ggtatgctgg tgatacgcaa caaaaagccc tacgtttttg aagcggtcgg cccggtgaaa
     8341 taccccccgc tcaagcagtg gatcgcccat ggtgaaaagg gcaaatacgt tgttcgccgc
     8401 gttgaaggcg gactgagtgt tgaacaacag caaaaactgg cgcaaacggc aaaacgttat
     8461 ctcggtaaac cgtatgattt cagcttctca tggagcgacg atcgccagta ctgttcggaa
     8521 gtggtgtgga aggtttacca gaacgcgctg ggaatgcgcg tgggcgagca gcagaagttg
     8581 aaagagttcg atctcagcaa cccgctggtc caggctaagc tcaaagaacg ctacggcaaa
     8641 aatattccgc ttgaggaaac ggtagtttca ccacaagccg ttttcgacgc gccacaactc
     8701 accacagtcg ccaaagagtg gccgctgttt tcgtggtaat ggatgatgaa aaaaataatt
     8761 cccgccgtag tctgtttgtt gctttgtagt tgcacgacct ttccgcaagc tgtcgctccg
     8821 gtaaacggtg atgcctccaa ttagttgaat taaggatata atactcgctt ttaaggttgt
     8881 ttcatggtca cagttaacct tcattgcccc cgttgccagt ccgttcaggt ttaccgtcac
     8941 gggcagaacc caaaaagaca tgagcgattc cgcggccgtg actgtcaccg ggtatttcag
     9001 ctcagttact gttacgagcc ccgtaaaccg ggagttaaag accaaatcgc tgaaatgact
     9061 ttcaacggtg ccggggttcg cgatactgcc cgtacgctga aaatcggcat taatacagtc
     9121 atccgaactt taaaaaactc tcgccacgaa gaataacgtc atccccggtc gctcatgctg
     9181 atgtggcact catctgcaaa cttagtcagc aatggagctt tgtcggcagt aaagcgcgac
     9241 agcactggct ctggtacgtg tacaacacga aaactggtgg tgttctggcc tacacttttg
     9301 gcccccggac tgacgaaacc tgtcgtgaat tgcgggcgct gcttaccctt ttaccatcgg
     9361 catgttgacc actgacgaat ggggtagtta cgctagagag ttaccgaagg agaagcatct
     9421 gactggtaag atttttactc agcacattga gcgtaacaat ctgaccctga gaacccgaat
     9481 caaacgtctg gcccgcagaa ccatctgttt ctcgcgttct gtagaacttc atgaaaaagt
     9541 catcggtgct tttatcgaaa atatat
//
LOCUS       DAZOYL010000145         9542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZOYL010000145 DAZOYL010000000
VERSION     DAZOYL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.145"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1321
                     /locus_tag="ACHUOY_002561"
     CDS             <1..1321
                     /locus_tag="ACHUOY_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0683313.1"
                     /translation="PQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHY
                     VLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCAR
                     AALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTLRV
                     MSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDH
                     NGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNPAE
                     IYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGFQN
                     RFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRVRV
                     RLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1324..2652
                     /gene="ssnA"
                     /locus_tag="ACHUOY_002562"
     CDS             1324..2652
                     /gene="ssnA"
                     /locus_tag="ACHUOY_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR0683314.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2703..3482
                     /gene="ygfM"
                     /locus_tag="ACHUOY_002563"
     CDS             2703..3482
                     /gene="ygfM"
                     /locus_tag="ACHUOY_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR0683315.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3479..6418
                     /locus_tag="ACHUOY_002564"
     CDS             3479..6418
                     /locus_tag="ACHUOY_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR0683316.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6351..6665
                     /locus_tag="ACHUOY_002565"
     CDS             6351..6665
                     /locus_tag="ACHUOY_002565"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0683317.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6701..7051
                     /gene="ghxQ"
                     /locus_tag="ACHUOY_002566"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6701..7051
                     /gene="ghxQ"
                     /locus_tag="ACHUOY_002566"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7120..7608)
                     /locus_tag="ACHUOY_002567"
     CDS             complement(7120..7608)
                     /locus_tag="ACHUOY_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0683318.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="ACHUOY_002568"
     CDS             complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="ACHUOY_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR0683319.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2301 a         2559 c         2488 g         2194 t
ORIGIN      
        1 tcctcagttc cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca
       61 cggcgtgaaa tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca
      121 gggcttccac tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc
      181 gggcgacaac caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac
      241 agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg
      301 cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc
      361 actacaagag atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga
      421 gttccgtttc ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt
      481 tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac
      541 agtaacactg catgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc
      601 cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg
      661 cgaaactcgt ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat
      721 tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat
      781 ccgttcccac cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg
      841 taaaggcgat atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca
      901 ggaagccgct cgctgcctcg aatgtaacta cgtttgcaac aagtgtgtgg atgtctgccc
      961 gaaccgcgcc aacgtctcca ttgcagtccc tggcttccag aatagattcc agacgctgca
     1021 cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa
     1081 accatacgaa gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcac
     1141 cccaggcttc cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgggt
     1201 gttaaacatc gacagcgaag gtcagtttaa caacgtacca ccggagctga acgatatgtg
     1261 ccgcatcatc agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta
     1321 atcatgttga ttctgaagaa tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa
     1381 ggcgttgata tcgctatcga aaatgacgtg attgtcgcta tcggcgatgc cctgacgcaa
     1441 cgctaccccg atgccagcta caaagagatg catggtcgga ttgtgatgcc gggaattgtc
     1501 tgctcgcaca accattttta ctcggagctt tcccgcggaa ttatggcaaa catcgcccct
     1561 tgcccggatt tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat
     1621 gaagagtcgc tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt
     1681 acatcggtta tcgatcacca tgcctcgccg gcgtatatcg gcgggtcgct ctccacattg
     1741 cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt
     1801 aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg
     1861 attgatgagg cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct
     1921 cacgcgccgt ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc
     1981 acaggccgtg gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac
     2041 cactggtacg gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa
     2101 acgctggtcg ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc
     2161 gatgcgttcc tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat
     2221 caccttagcg acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt
     2281 gaagagatga aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac
     2341 agttttgcca aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa
     2401 tttgggcttc tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg
     2461 acgccgctgc tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc
     2521 gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat
     2581 tgcgattcca tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat
     2641 gcgctggcat aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga
     2701 agatgattga acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc
     2761 gctaccagga tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc
     2821 gtaccgataa aaagattgcc atttccttac aggatctgga actggactgg gttgactggg
     2881 ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca gccactgcgt gatgcgcgct
     2941 ttattcctgc agcactgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc
     3001 agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga gtcagtgctg cttcccgtcc
     3061 tgctagcact ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact
     3121 acctggcctg cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca
     3181 cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg
     3241 ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac
     3301 tgcgtctgca cgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg
     3361 ccaacgccat tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca
     3421 cgggagttct ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat
     3481 gatcatccac tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa
     3541 cgtgcaaaag ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggctt
     3601 cgggtttgcc ggttctgacg caataatctt taacggtaat atcgttaacg cgtccttgct
     3661 tattgccgca cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa
     3721 cgagttaagt ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa
     3781 cgatccagct gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg
     3841 cgaagagatc gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata
     3901 ctatcaggtc attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat
     3961 cgctccgact ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc
     4021 cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac tgcgtaacgg ctgacgcctg
     4081 cgtcattaaa atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag
     4141 caaagctgaa gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat
     4201 ctactatacc ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt
     4261 cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga
     4321 aattgcgctc gaagcactga agctcatcga cgttgaatat gaagtgctta agccggtaat
     4381 gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta
     4441 tgttgctggt gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga
     4501 gcatatgatc atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat
     4561 tcatggtcat attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg
     4621 aacctataac tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg
     4681 gatggacggc gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg
     4741 ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt
     4801 tggcggcggt tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac
     4861 ctgcgtgacc gggcgtccgg tactattccg ctacacccgt gaagaagagt ttattgctaa
     4921 cacctctcgt cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct
     4981 gacggcagtg aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac
     5041 cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt
     5101 ccaggtcacc acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc
     5161 accgaaaggt aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat
     5221 cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct
     5281 cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact
     5341 ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa
     5401 tggtgactgg cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga
     5461 tatcgatcag gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc
     5521 tggcggtgcg gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt
     5581 gctgcactgc ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt
     5641 tgataaaggc gcatatgcct cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc
     5701 agcggaaaat ctacgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt
     5761 ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt
     5821 cggggatatt gcccataaag gcgaaaccgg catcggcttt ggttcactgg tgggaactgg
     5881 cagttatatc acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt
     5941 caacacgcgt acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac
     6001 accggtcaac ccggaattag cattgggaca aatctacggt gctaccctgc gagctatcgg
     6061 ccacagtatg agcgaagaga tcatttatga cgccgaaggt cacccgttaa cgcgtgattt
     6121 acgcagttac ggcgcaccga aaattggtga cattccgcgt gatttccgcg ctgtgctggt
     6181 gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg atctcggaaa tcggtgtaaa
     6241 tggcgcagct ccggcgattg ctaccgcaat tcacgataca tgcggcatct gggcagaaga
     6301 aagtcaaagt gggcatggga acggatatcg gtgccggaac aactttcaac atgctgcaaa
     6361 cgctgaacga agcctacaaa gtgttgcaat tacaaggcta tcgcctctcg gcatatgaag
     6421 cgttttacct ggccacgctc ggcggagcga aatctctggg ccttgacgat ttgattggca
     6481 actttttacc tggcaaagag gctgatttcg tggtgatgga acccaccgcc actccgctac
     6541 agcagctgcg ctatgacaac tctgtttctt tagtcgacaa attgttcgtg atgatgacgt
     6601 tgggcgatga ccgttcgatc taccgcacct acgttgatgg tcgtctggtg tacgaacgca
     6661 actaataata aaactttaac atcctcgtga ggacatcatt atgtctggag acatcctaca
     6721 aacaccggac gcaccaaagc cacagggcgc gctggataat tattttaaaa ttaccgctcg
     6781 tggcagtacc gttcgtcagg aagtactggc tggcttaacg acctttctgg ccatggttta
     6841 ttccgttatc gtcgttccgg gaatgctggg caaagcagtt tttcctcccg cagctgtgtt
     6901 tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg atggggttgt aggccaattt
     6961 gccaatggcg attggttgtg cgatttcctt gacggcattt accgcattca gtctggtact
     7021 cgggcaacaa attagcgttc ctgtcgcact ggtcgcattt tacgcgggtg gttgggcaat
     7081 ctaatagatt ctccgcgcct ttcagcgcgg agatttcttt cacagaggat tctcaccgct
     7141 ggcaacaata cgctgctggc ggactcgcct gagttctctt tcagtaagca actgcagcgc
     7201 ctgtgtcggg caagattcaa cacaggctgg cccctcttcc cgctgttcac agagatcgca
     7261 tttcacaatt tgttgtcggg tatcccccgg caacgactga atggtgatca ttccaaacgg
     7321 gcacgcactg acgcaactct gacagccaat acaacgggca gaattggtct gcaccacctg
     7381 ctcgcccatc gtcagcgccc ccacggggca agcgccaaca caaggggcgt tttcacactg
     7441 atggcacatc accggcgcac tgatgctgtc cagtcgctgt accttcagcc ggggtaaaaa
     7501 gacatcggca ttcaactcct gttctgaagg gtgagcgacc acacaggcta cttcacaagt
     7561 acggcagcca atacagtcag ccggattaac gataattaac gatttcatca cgatgccttc
     7621 gtatcaaaca gagttaacat atcgcgcgcc gcctgccttc ctgcggccat tgcagtgaca
     7681 accagatccg cgccatgaac tgcatcacca ccagcaaaga cttttttcag atgcgtctgg
     7741 gtaggtaaat acccgacgtc accggtttga atcaggcccc atttatcgag tttaattccg
     7801 ctgccctgca accacggcat ggcatgcgcc tggaaaccaa aggccataat cagaacatcg
     7861 gcgggcaatt caaactctga acccgctacc ggacgaggac gacggcgacc atccggcccc
     7921 ggctcaccca tggcggtacg aatcaggccc accgcagtta aacgcccatc ttcgtcacaa
     7981 gcgatatatt gcggttgaac attgaactga aactcgacac cttcctcgcg cgcattaacc
     8041 acctctttgc gcgagcccgg catactgact tcatcacgac gatacgcgca ggtcacgctg
     8101 gcagcattga ggcgaatgga agtccgcaaa caatccattg tcgtatcgcc accgcccaat
     8161 accacgactc gcttaccttc cacgtccgtc agcggatact cttcagactc cggcaatccc
     8221 atgagctggc gggtatgggc agtcaggaac ggtagagcct gaatgacacc gggcgcatct
     8281 tcatgcggca gatctgctcg catcatcccg taagtcccca cgccgatgaa aactgcatca
     8341 tattcagaag ttaaatcgct aaaggtaata tcgcggccaa tttcacagtt aagatgaaag
     8401 tcgattccca ttgcggtgaa tatctctcgc cgctggctta ataccgtttt atcgagtttg
     8461 aaaggaggaa tgccaaaagt cagcataccg ccaatttctg gatggcgatc aaagacatca
     8521 acctgaactc ccgcgcgcgc cagaatatca gcacacccta accctgctgg cccagcgcca
     8581 atcaccgcca ctttttcgat acggggaaca actttgctga catcgggacg ccagcccatc
     8641 gccagcgcgg tatcggtgat gtagcgttcc agattaccga tagagactgc gccagagtga
     8701 tctttcaaag tacatgcgcc ttcacaaaga cggtcctgtg ggcataccct gccgcagatt
     8761 tctggtaagg aactggtctg gtggcaaagt tctgccgctt caataatctt tccttcctgt
     8821 accagacgga tgtaatccgg aatagcgtta tgcagcgggc aatgccagtt gcagttagct
     8881 ttttcggcac aataaacaca gcggtcactc tcataagtcg cttgcagtgg atccagcccg
     8941 caatagattt cgccaaagtg ggttttccgt tcactcgctg aaattttatc tgcgccttta
     9001 cgcgagttaa cggggagcaa cgctgcactg cgagatggct gagcgtctga tgacgctttt
     9061 cctgctgccg ttttgcgctg gcgagccacc tttatccact gtaacccttt atcgtccatc
     9121 agtcgtaacg cctgtgttgg gcagacttca atacaggctt gcgtgccgga actgcgctgg
     9181 ttacaaaggt cgcatttctg cgcaatcgta tcgaccatct caacgacgcc aaaggggcaa
     9241 gcgattgcgc atcttttaca gccaatacat ttttgctcgt ccagttgtac gctatcggac
     9301 tggaaagtca gagcattaac cggacaagcc gtaacacaag gggcattgtt gcagtgatgg
     9361 caggccaccg gattcgcagc ctggcctttc cctacaacgt ggatacgcgg tcgaaagtca
     9421 ctgtgactca gcggccagtt ttcttgattg tgtgccaccg cacaggcaat ttcacaagca
     9481 tgacagccta tacattccgc agcttcagca gcgataaact tattcatttg catccctttc
     9541 at
//
LOCUS       DAZOYL010000146         9519 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZOYL010000146 DAZOYL010000000
VERSION     DAZOYL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.146"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHUOY_002569"
     CDS             complement(<1..716)
                     /locus_tag="ACHUOY_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0683320.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUOY_002570"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUOY_002570"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR0683321.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUOY_002571"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUOY_002571"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR0683322.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHUOY_002572"
     CDS             complement(3650..4705)
                     /locus_tag="ACHUOY_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0683323.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHUOY_002573"
     CDS             complement(4715..5503)
                     /locus_tag="ACHUOY_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR0683324.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHUOY_002574"
     CDS             complement(5521..6573)
                     /locus_tag="ACHUOY_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR0683325.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7207)
                     /locus_tag="ACHUOY_002575"
                     /pseudo
     CDS             complement(6604..7207)
                     /locus_tag="ACHUOY_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7194..8075)
                     /locus_tag="ACHUOY_002576"
     CDS             complement(7194..8075)
                     /locus_tag="ACHUOY_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0683326.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8096..9406)
                     /locus_tag="ACHUOY_002577"
     CDS             complement(8096..9406)
                     /locus_tag="ACHUOY_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0683327.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9441..>9519)
                     /locus_tag="ACHUOY_002578"
     CDS             complement(9441..>9519)
                     /locus_tag="ACHUOY_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFR0683328.1"
                     /translation="NITNLHGRDLISEVDLLGNEITLRP"
BASE COUNT         2212 a         2682 c         2397 g         2228 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac aggcagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttacctcttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cacttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc agcccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtcgac gtaatgctcc agcgtccact gctgcggcag cagcgtagga tgcagtgaga
     7081 tcgcctcttt cgcgcccttg aacgaggtca tcagcatcac aaaaaatgga aacagcgtga
     7141 tgatgagaaa cagcgccagc ccgcagtaaa aaccgatgcg actgagtgtg cgtttatttg
     7201 ttgccattga ggttcacccg tttcctggtc agcagaatga cagcgaaaat aatgatgaag
     7261 agcaccaccg aaatcgccgc cgctttccct aagtcattaa aggcaaaggc ggttttgtag
     7321 agatagacgc cgagaatatc gaccttggtg gtcagcagat aaacatcagc aaacatgtag
     7381 aacatccaga tagtgcgcag ggtcaccacc gtcgccagga ccggcataat tgcgggcagc
     7441 gtgacgatgc gaaaccgctg ccaggcatta gcgccatcca tctctgccgc ttcgtacagc
     7501 gatttgtcga tggtctgcaa aatcgccaga aacgagataa aggcatacgg gaagtagcgc
     7561 cagatggcga acagcaccac cagcacaaag ctactgcccg gattatcgaa ccacagcggt
     7621 gcctgctcat agagatgcag aagatcgacg ccgaggtagt tcacaatgcc gtagccgttg
     7681 ttgaacatgt atttccaggc aaacaccagc gaaatggacg gcgttacgta ggagaggatc
     7741 accagcgaac gcgcggtttt gcgcaggcgg aactcgcggt tgaaaaacat cgccactgcc
     7801 agcccgagca cggtgctgcc cgccaccacc agcgcggtat accagaccgt catccacagc
     7861 gagtgccaga agccgggatc ggagaggata cgcacatagt tactcacccc aacaaaggtt
     7921 gactcgatgt tgggattgag cggcagacgt aaaaaactga tttcgatatt cgacaccatt
     7981 ggccaggcca ccagaccacc cagcagtaat aagctgggcg cgagaagcag cagcgcaaag
     8041 ggcatatcgg aacgacctga aaaaagcctg ttcattgcgg attcctgtcc tgggtttagt
     8101 gctgttcgat cagttcatcc agctttttct ggctcgcttg cagcgtgcca gggagatcgg
     8161 ctttacccac ggtgacgtta tgcaccatgg agctcaccac gccagaaccc gtcacatcac
     8221 ccatgcgggt aaagttttta tcccctactg cgccaaagac ctgaatattt ggcagttcac
     8281 cgattagctg attcggtagt tcacccagcg ccttaataac gtcgttgtct ttccaggtgg
     8341 cggtagtgac aaccgcttta ttcaccggca gcgcagcacc tggcgacatc atcacccagt
     8401 cggcaatgtt gtctgcctgc tccataaagg tgacaaattt ttctgctgct tccgtctctt
     8461 cggccttttg cccggcggta atggtcagcg aggtcaacat gccgtagacc gcagagtttt
     8521 tctcggttgg caccacgaaa ccgacgtttt tcgggtcgcc ttctttaatt acagccggaa
     8581 ggatataggt ggagtaaatc gccatcggcg cggtgccgtt cataaaggcg tctttcactt
     8641 ccatgatgtc gttagaaccc ggcatgtcgt tagaacccgg catagtgttg gcagcaaggt
     8701 cgcggtaata ggtcagtgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
     8761 cgttaaagac attagcctgg ttggataacg caaactggga gaaggattgt tccgtcaaca
     8821 cgctttctgc tgtaggcagc gcaatgccgt attttttatt cgccgggtca ttcagtttct
     8881 gtgcaacgtc cagtagctgt tgccagtttt tcggctcctc cagccccgct tttgccagca
     8941 catctttgcg ataccagatg ccgccaatcc aggcgctgac gggaacaccg gtccaggcac
     9001 tcccatcttc ggtgcgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt
     9061 tgctgatgac cgtggcaacc gtcttgcgat cgataagctg ctctttatcc ataactttgg
     9121 cgtagtcatg gctggtttcg atcacttccg gcagcgagcc gctacgtgaa agggtgatga
     9181 ctttggtgtt ataagcgtcc tcttccacgg gcacttgttt aacggtgata cctgggtttt
     9241 ctttttcaaa acgggcaatc aatttgctga taactgcctg acgctcctgc tccaccgaag
     9301 aatgcataaa ttcgatggat acatgggttt tattttcttc tttacagcct gaaatcaggg
     9361 cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaga tgttcctttt
     9421 tatttaatcc acataacctg ttaggggcgc agcgttattt cattacccaa tagatcaact
     9481 tcactaatca gatcccgacc atgcagatta gtaatattt
//
LOCUS       DAZOYL010000147         9454 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZOYL010000147 DAZOYL010000000
VERSION     DAZOYL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.147"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            237..1424
                     /gene="ygeW"
                     /locus_tag="ACHUOY_002579"
     CDS             237..1424
                     /gene="ygeW"
                     /locus_tag="ACHUOY_002579"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR0683329.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            1482..2678
                     /gene="dpaL"
                     /locus_tag="ACHUOY_002580"
     CDS             1482..2678
                     /gene="dpaL"
                     /locus_tag="ACHUOY_002580"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR0683330.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            2736..3947
                     /locus_tag="ACHUOY_002581"
     CDS             2736..3947
                     /locus_tag="ACHUOY_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR0683331.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            4000..5384
                     /gene="hyuA"
                     /locus_tag="ACHUOY_002582"
                     /pseudo
     CDS             4000..5384
                     /gene="hyuA"
                     /locus_tag="ACHUOY_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            5432..6364
                     /gene="arcC"
                     /locus_tag="ACHUOY_002583"
     CDS             5432..6364
                     /gene="arcC"
                     /locus_tag="ACHUOY_002583"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0683332.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFVRSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6405..8030)
                     /gene="yqeB"
                     /locus_tag="ACHUOY_002584"
     CDS             complement(6405..8030)
                     /gene="yqeB"
                     /locus_tag="ACHUOY_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR0683333.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(8078..8848)
                     /gene="yqeC"
                     /locus_tag="ACHUOY_002585"
     CDS             complement(8078..8848)
                     /gene="yqeC"
                     /locus_tag="ACHUOY_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR0683334.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            8951..>9454
                     /locus_tag="ACHUOY_002586"
     CDS             8951..>9454
                     /locus_tag="ACHUOY_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HFR0683335.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIE"
BASE COUNT         2459 a         2496 c         2309 g         2190 t
ORIGIN      
        1 ataaataacg cgataaaaaa agccaaacgt tttcgtattt tacaaacaac cagaagctgg
       61 catcaatttg tgaacaaccc cacacattat ccgtcaaatt agtcttttgc agccgcgcgg
      121 ataattctgg cacacttatt gttagtccca ggtatagcta tgaaaacacc aatcactttg
      181 gcaagtcaca gtgaaataaa ccactttgcc tgtcattcca ctaccgggac tgtatgatga
      241 aaactgttaa tgagctgatt aaggatatca attcgctgac ctctcacctt cacgagaaag
      301 attttttgtt aacgtgggaa cagacgccag atgaactgaa acaagtactg gacgttgccg
      361 cagcattaaa agcactgcgt gctgaaaaca tctcaaccaa agtctttaat agtggattag
      421 gtatttccgt attccgcgac aactccaccc gtacccgctt ctcttatgct tccgcgctta
      481 acctgctcgg ccttgcacaa caagatctcg acgaaggcaa atcacaaatc gctcacggcg
      541 aaaccgtgcg tgaaaccgcc aatatgatct ccttctgcgc cgacgctatt ggtattcgcg
      601 acgatatgta tctgggcgca ggcaacgcct atatgcgtga agttggcgct gcacttgatg
      661 acggttacaa gcagggtgta ctgccacagc gtccggcttt agtgaacctg caatgcgata
      721 ttgaccaccc gactcagtca atggctgacc tcgcgtggtt acgtgaacac tttggttcac
      781 tggaaaacct gaaaggtaaa aaaatcgcca tgacctgggc ctactctcca agctatggca
      841 aaccgctctc tgtaccacaa ggcatcatcg gtctgatgac tcgcttcggt atggatgtca
      901 ccttggccca tccggaaggc tacgacctga tcccggatgt ggttgaagtg gcgaaaaaca
      961 atgctaaagc ctccggtggt agcttccgtc aggtcaccag catggaagaa gccttcaaag
     1021 acgcagacat cgtttatccg aagtcatggg caccttacaa agtgatggaa gagcgtactg
     1081 aattgctgcg tgcgaacgac cacgaaggct taaaagcact ggaaaaacag tgtctggcac
     1141 agaacgcgca acacaaagac tggcattgta ctgaagagat gatggaactg acccgtgatg
     1201 gcgaagccct gtacatgcac tgcctgccag ctgatatcag cggcgtatcc tgcaaagaag
     1261 gtgaagtgac tgaaggcgta ttcgaaaaat accgtatcgc tacctacaaa gaagccagct
     1321 ggaagcctta tatcatcgcc gcgatgatcc tgtcccgtaa atacgccaaa ccaggtgcac
     1381 tgctcgagca actgctgaaa gaagcgcaag aacgcgtgaa ataacccctc gggccgatct
     1441 gctggtcggc cctctatttc cagaggccaa aaggatagga tatgtccgtt ttctcattga
     1501 agattgatat cgccgataac aaatttttca acggcgaaac atcaccgctc ttttcgcaaa
     1561 gccaggccaa actggcgcgc cagttccacc agaaaatagc tggttatcgc ccaacaccgc
     1621 tttgtgcgct ggacgatctc gcaaaccttt ttggtgtgaa gaaaattctc gtcaaagacg
     1681 aatcaaaacg attcggtctg aacgccttca aaatgcttgg cggtgcgtac gccatcgctc
     1741 aattattgtg tgaaaaatat catcttgata tcgaaacgct gtcatttgag cacctgaaaa
     1801 atgccatcgg cgaaaaaatg actttcgcga ccaccaccga cggcaaccac gggcgcggtg
     1861 tggcgtgggc agcacagcaa ctcggacaga atgcggtgat ttacatgccg aaaggttctg
     1921 ctcaggaacg cgttgacgcc attctgaacc tcggtgccga gtgcatcgtc acggatatga
     1981 actatgacga taccgttcgc ctgaccatgc aacacgcgca gcagcacggc tgggaagtgg
     2041 tacaggacac ggcatgggaa ggttacacca aaatcccaac ctggatcatg caaggctacg
     2101 caaccctggc agatgaagcc gtcgagcaaa tgcgtgaaat gggcgtaacc ccgacgcacg
     2161 ttctgctgca agccggtgtc ggagcaatgg ccggtggtgt gctgggttat ctggtcgacg
     2221 tctacagccc gcaaaatctg cacagcatta ttgttgaacc tgacaaagct gactgtattt
     2281 atcgctccgg cgtcaaaggc gacatcgtca acgttggcgg tgatatggcc accatcatgg
     2341 cgggcctggc ctgtggcgaa cctaacccgc tgggctggga aatcctacgt aactgcgcca
     2401 cccaattcat ctcctgccag gacagcgttg ccgcattagg tatgcgcgtg ctgggtaatc
     2461 cgtacggcaa cgacccgcgc atcatctccg gtgaatccgg cgctgtcggt ttgggcgttc
     2521 tcgcagcggt tcattatcac ccgcaacatc aaagcctgat ggaaaaactg gcgctggaca
     2581 aagatgccgt agtgctggtt atcagcactg aaggcgacac cgacgtgaag cactaccgcg
     2641 aagttgtctg ggaaggcaaa cacgctgtag caccttaatc accctattgg aactactccc
     2701 tgcaaaacgg ggagtaaaaa atctggagaa aaataatggc taagaatatt ccattcaaac
     2761 tgattcttga aaaagcaaaa gattaccagg cggacatgac tcgcttcctg cgcgacatgg
     2821 ttgctattcc cagtgaaagc tgcgacgaaa aacgcgtagt acatcgtatt aaagaagaga
     2881 tggaaaaagt cggcttcgat aaagttgaaa tcgacccgat gggcaacgtt ctcggttata
     2941 tcggccacgg cccgcgtctg gtggcaatgg acgctcatat cgataccgtc ggcattggca
     3001 acatcaaaaa ctgggacttc gatccgtacg aaggcatgga aactgatgag ctaatcggtg
     3061 gtcgcggtac ttccgaccag gaaggcggca tggcatctat ggtttatgcc ggtaaaatca
     3121 ttaaagacct cggtctggaa gatgaatata ccctgctggt taccggtact gtgcaggaag
     3181 aagactgcga cggtctgtgc tggcagtaca ttattgaaca atccggcatt cgcccggaat
     3241 ttgtggtcag taccgaacca accgactgcc agatataccg tggtcagcgc ggtcgtatgg
     3301 aaattcgtat tgatgttcag ggtgttagct gccacggttc tgcgccagaa cgcggtgaca
     3361 acgccatttt caaaatgggt ccgattcttg gcgaattaca agaactctcc caacgtctgg
     3421 gttatgacga attcctcggc aaaggcaccc tcaccgtttc tgaaatcttc ttcacatccc
     3481 caagccgttg cgctgtagca gatagctgcg ccgtctctat tgaccgccgt ctgacctggg
     3541 gcgaaacctg ggaaggcgcg ctggacgaaa tccgcgccct gcctgcagta cagaaagcta
     3601 acgcggttgt ttctatgtac aactacgacc gtccgtcctg gactggcctg gtttacccaa
     3661 ccgaatgcta cttcccgacc tggaaagtgg aagaagatca cttcaccgtt aaagcactgg
     3721 tgaatgccta cgaagggctg tttggcaaag cgccggttgt tgataagtgg accttctcaa
     3781 ctaacggcgt atctatcatg ggccgtcacg gcattccggt gatcggcttt ggcccgggta
     3841 aagaacctga agcgcatgca cctaacgaaa aaacctggaa atctcacctg gtgacctgtg
     3901 ccgcgatgta cgctgcaatc ccgttaagct ggctggcaac agagtaatta ctcttcactt
     3961 attcccctcc ggttcgccgg agggtttttg gagtttgcta tgcgcgtatt gatcaaaaac
     4021 ggcactgtcg ttaacgcaga tggacaagcc aaacaggatt tgctgattga aagcgggatt
     4081 gttcgccagt tgggcaacaa tatttcgccg cagctcccgt atgaagaaat tgatgccact
     4141 ggctgttacg ttttccctgg cggcgtggat gtccatacgc atttcaatat tgatgtcggc
     4201 atcgcgcgca gttgtgatga ttttttaccg gtacccgcgc agctgcgtgt ggcggtacaa
     4261 caaccattat tgaccatatg ggatttggcc caaacggctg tcggttacgc catcaactgg
     4321 aggtttatcg tggttatgcc gcccataaag cggtcatcga ttacagcttt cacggtgtga
     4381 tccagcacat taatcacgca atcctcgacg aaatcccgat gatggtcgag gaaggactga
     4441 gcagttttaa actctattta acctatcaat acaaactcaa cgatgacgag gttttgcagg
     4501 cattacgccg cttgcatgaa tccggcgcgc tgaccaccgt gcacccggaa aatgatgcgg
     4561 ctatcgccag caagcgggcg gaatttatcg ccgcagggtt aaccgcgccg cgctatcatg
     4621 ccttgagtcg ccctctggaa tgcgaagcgg aagccatcgc ccgcatgatt aacctggcac
     4681 aaattgccgg taacgccccg ctctatatcg tgcacctgtc taacggctta ggtctggatt
     4741 atctgcgtct tgcccgtgcg aatcaccagc cagtctgggt tgaaacctgc ccacaatatc
     4801 tcctgttgga cgaacgcagt tacgatacag aagatggcat gaagttcatt cttagcccac
     4861 cgctgcgtaa cgtacgcgag caggacaaac tgtggtgtgg catcagcgat ggtgcgattg
     4921 acgtggtggc gaccgatcac tgcaccttct cgatggctca acgcctgcaa atttctaaag
     4981 gcgatttcag tcgctgccca aatggcttac ccggtgtgga aaaccgcatg cagttactgt
     5041 tttccagtgg cgtgatgacg ggacgtataa caccggaacg ctttgttgaa ttaaccagcg
     5101 caatgcccgc caggctgttt ggcctgtggc cgcaaaaagg attattagcg cccggttccg
     5161 atggcgacgt ggtgattatc gacccacgtc agagccaaca aattcagcat cgccatctcc
     5221 acgacaacgc cgactactcg ccatgggagg gttttacctg tcagggcgcg attgtcagaa
     5281 ccttatcccg tggtgaaacg attttctgtg acggcacctt tacaggcaaa gccgggcgag
     5341 gtcgtttcct gcgacgcaaa ccgtttgtcc ctcccgtgct ctaaccactg tcagggatct
     5401 atacacaatc aagaatgatg agtgaggaaa aatgagtaag aaaattgttc tcgccctggg
     5461 cgggaatgcg ctgggcgatg acctggccgg acaaatgaaa gcggtaaaaa ttacttctca
     5521 ggcaattgtt gatttaattg cccaaggaca tgaagttatc gtcactcatg gtaacgggcc
     5581 gcaggtgggc atgattaacc aagcctttga agctgccgca aaaactgaag cacactcgcc
     5641 aatgctgccg atgtctgtct gtgttgcctt aagccagggt tatattggtt acgatctaca
     5701 aaacgcgtta agagaagaac tgctttctcg gggcattaat aaacctgtag caacgctggt
     5761 tactcaggtc gaagtcgacg ctaacgatcc ggcattcctc aacccgacca agccgatcgg
     5821 ttcgttcttt accgagcaag aagcagagca actgacaaaa cagggttaca ctctgaaaga
     5881 agatgctggt cgcggctatc gccgtgttgt tgcctcgcca aaaccggttg atatcattga
     5941 aaaagaaacg gttaaagctc tggtagatgc aggccaggtg gtgatcgccg ttggcggtgg
     6001 tggtatccct gttattcgtg aaggtaacca tctgcgtggt gccagcgcgg ttatcgataa
     6061 agactgggcc agcgcccgtt tagcagaaat gattgatgcc gatatgctga tcattctgac
     6121 tgcggtagaa aaagtggcca ttaacttcgg taaagagaat gaacagtggc tcgatcgcct
     6181 gtcattaagt gatgctgaac gctttatcga agaaggtcat tttgcgaaag gttctatgct
     6241 gcctaaagtg gaagctgccg cctcgtttgt tcgctctcgc gcaggtcgtg aagcactgat
     6301 taccgtattg agcaaagcga aagaagggat tgaaggcaaa accggaacgg tgatttgtca
     6361 gtaattttaa ggccgggtgg gtaataccat ccggccattt tcatttaagc cacttccagc
     6421 acttcttttg tcgctttcac gccctgatgc atcaacatca ttaacgcctc aagtacgccg
     6481 ccgccaatcg cccgggcttt atcagaaacg ctggtgaaat cagcggtttc accacgagga
     6541 tcgatatcac caattttaaa accaccaacc actgccaggc cgtcgttcaa caagccacgc
     6601 accatccccg tcaacggtgc tttaatttca tgctcaccaa tccaggcaat cacatcgccc
     6661 tctttcacca gatcgcctaa tttcacgttg gatcgcataa tgcctgcagc aggagcacgg
     6721 atcacccgtc gggtggtatg ccccataata ttgccaggaa catcggtatt ctcctgcgca
     6781 caaccagagt aaatcacctg accgagccag tgcccgcgat ttgtttcaat taccgcatga
     6841 caatccttcc ctgcagtaaa gcccggccca agcgcgattg ttactggtgc catatctgcc
     6901 cgcgttccca aattctgttt cgccagaata gcgtccacca cgcaaagcgg tttcagttca
     6961 tcaagcaatg aacaggcggg atctaccatc acagggatga atccgcgttc ggtaagtttc
     7021 atcgcttccg cagagctggt tgccaggcga gcagtgacgc cttcgaccgt catttcgcca
     7081 tcgaacacgg cctgggcaaa cgccacggta caacgaatca ctgtcggttt ttccacttcc
     7141 agcatgatca ctttaaaacc cgcatgatac agacgtagcg ccacaccact ggcgatatct
     7201 cccgcaccgc gaatcaccac cagctggtgt ccggaaggat gagaaggttt catcatcagc
     7261 ccacccggcg cgttattttt cacccataat atttctgcca gcacgctgat ggcgatctcc
     7321 tgcggcgttt ccgcacctat gttgtaacca acgggcgcat gtaaacgggc aatatgttct
     7381 tcagccacgc ctttctcacg caattgacgc aggaaaagct gaaccttgcg gcgacttgcc
     7441 agcaacccca accatgcaat gggctgttca atgagtttgt cgagggcttc acgatcctga
     7501 ttattcgtgg caatcaggac aaaattatca gggcgaatat ccagttcttc cactgccgca
     7561 ccaaacgact cagcatggag aagcgtggtt gatggtggga atagttcagg attgaggctt
     7621 tcgcgataaa tatcggcaac ggcaatatca aatcctaata gcgccgcact ctgggctatc
     7681 gcccggttga catgccccgc gccaatcaac accagacgtg gacgcatacc atggacgctg
     7741 ataaatactg acattgcacc tccacaatct gacccgacag catccgcacc gttacgagcc
     7801 atacgtccat ggaataatcg cggcttacgt tcctgcaatg cctgaagtga ctcttcaatc
     7861 accttccgct caaccattcc gccaccaatt gtaccgacga tagaaccatc ggcgcgcact
     7921 aacatttgtg cagaatggcg gggagtcgag cctcggctat caacaatttg cgccatcgca
     7981 aacggacaat tttgctcttc gagttttgca gcctctgtga aaatattcat accaacctca
     8041 atggatatcc tttcagtaac ccggaatacc cgggccgcta attcacaaat ctgcgcgcga
     8101 ttgcaggatg ttcttgtata tcgcccagcc agattgcctc tacgtcgtgt tgttgcagcg
     8161 gttgaaggag ctcgctttgt gcaatcgcat tctcacattg agaaaaacgg ttaatgaacc
     8221 agactcgccg acaaccttgc ggtacgtttt taaacgcccc ctgagggtgg cgaaccagcg
     8281 caacgagatc gctcagttgc aaggttgcat caggtgttaa cccggtaata tcagcaaact
     8341 gcgaccagcg atggacattt tctgtgctca ctttcgcgcc caaagtatgt cctcccatca
     8401 cagcaatcac gcagcaactg cttttgggta tgcaaggttc gtgctcatca ggcgctttta
     8461 acggcattcc acgcgagcca tctgcttcaa tgagaattac gtcacactct ggtcgttgca
     8521 ccagtgcatc aatcgcttct ggcgtaaatc cctgcacttt tccctggttc gctttccagc
     8581 tgtgaaaaca aaatgaaatg ggggatgtaa gagacgcatg aggaagcatg gcgggatcac
     8641 gacagaaaac cacgggccag tgagacgttg gcataaacat atgtgtcgtc gtcgtgatta
     8701 acacacgcct gccgctagcc tggagcagtt ctgccagcca aaaaagcaag ctggttttcc
     8761 ccccagcacc aacaacagaa atcacagtgg gacgtttctg cgcacctaaa tcaatgacta
     8821 atgccgatgg gtcaactata cttttcacga gtctttatga cctctctggg ataaattatc
     8881 cccaacttaa tccatcagga agtaacgcaa ttatcaggcg ttattagccc ctataaataa
     8941 tggaaccact atgtcagcca tcgactgtat aattaccgct gccggattat catcaagaat
     9001 ggggcaatgg aaaatgatgt tcccctggga acagggaaca attcttgata caagtatcaa
     9061 aaatgcgttg cagttttgta gccgaattat tttagtcacc ggctatcgtg gtaatgaact
     9121 gcacgaacgt tatgcgaacc agagcaatat cactattatt cacaacccag attatgcgca
     9181 gggtttacta acatccgtca aggccgcagt acccgcagtg caaacagaac attgttttct
     9241 cacccacggt gacatgccaa ccctcaccat cgatattttt agaaaaatct ggtcgttacg
     9301 aaatgatggc gcaatactgc cactccataa tggcatcccc ggccatccga ttttagtgtc
     9361 aaaaccatgc ctgatgcagg caatccagcg acccaatgtc accaatatgc gtcaggcact
     9421 ccttatggga gatcattatt ccgtcgaaat agaa
//
LOCUS       DAZOYL010000148         9432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZOYL010000148 DAZOYL010000000
VERSION     DAZOYL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.148"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="ACHUOY_002587"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="ACHUOY_002587"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR0683336.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUOY_002588"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUOY_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR0683337.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="ACHUOY_002589"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="ACHUOY_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUOY_002590"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUOY_002590"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR0683338.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUOY_002591"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUOY_002591"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR0683339.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUOY_002592"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUOY_002592"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR0683340.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="ACHUOY_002593"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="ACHUOY_002593"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR0683341.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="ACHUOY_002594"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="ACHUOY_002594"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR0683342.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="ACHUOY_002595"
     CDS             complement(6556..7449)
                     /locus_tag="ACHUOY_002595"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0683343.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="ACHUOY_002596"
     CDS             complement(7446..8099)
                     /locus_tag="ACHUOY_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0683344.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="ACHUOY_002597"
     CDS             complement(8110..9369)
                     /locus_tag="ACHUOY_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0683345.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2159 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAZOYL010000149         9407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZOYL010000149 DAZOYL010000000
VERSION     DAZOYL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.149"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            370..801
                     /gene="osmC"
                     /locus_tag="ACHUOY_002598"
     CDS             370..801
                     /gene="osmC"
                     /locus_tag="ACHUOY_002598"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR0683346.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(857..1747)
                     /locus_tag="ACHUOY_002599"
     CDS             complement(857..1747)
                     /locus_tag="ACHUOY_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR0683347.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(1776..2762)
                     /locus_tag="ACHUOY_002600"
     CDS             complement(1776..2762)
                     /locus_tag="ACHUOY_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0683348.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            complement(2759..3655)
                     /gene="ddpC"
                     /locus_tag="ACHUOY_002601"
     CDS             complement(2759..3655)
                     /gene="ddpC"
                     /locus_tag="ACHUOY_002601"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR0683349.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3652..4674)
                     /locus_tag="ACHUOY_002602"
     CDS             complement(3652..4674)
                     /locus_tag="ACHUOY_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0683350.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(4676..6226)
                     /locus_tag="ACHUOY_002603"
     CDS             complement(4676..6226)
                     /locus_tag="ACHUOY_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0683351.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(6240..6821)
                     /gene="ddpX"
                     /locus_tag="ACHUOY_002604"
     CDS             complement(6240..6821)
                     /gene="ddpX"
                     /locus_tag="ACHUOY_002604"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR0683352.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            complement(7080..>9407)
                     /gene="dosP"
                     /locus_tag="ACHUOY_002605"
                     /gene_synonym="pdeO"
     CDS             complement(7080..>9407)
                     /gene="dosP"
                     /locus_tag="ACHUOY_002605"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR0683353.1"
                     /translation="AVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDMLIPRDLRPA
                     HPEYIRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLALVRDAS
                     VEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQCNRAFTEMFGYCISEASGMQPDTL
                     LNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVYDVLAHLQNLV
                     MTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGM
                     PIHWASSSHGAEVQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHM
                     AALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDKAVSPVVYLIGVDHIQD
                     VIDSLGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIADEL
                     RNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSP
                     AMNEMVKERLVLGAALKEAISNNQLKLVYQPQIFAETGELYGIEALARWHDPQHGHVP
                     PSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPALSVNLSALHFRSNQLPN
                     QVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGL
                     SRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEM
                     LRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLKI"
BASE COUNT         2300 a         2441 c         2388 g         2278 t
ORIGIN      
        1 tttctgcctg ataataagta aacatagtga ttctccgtgt ctgtgtattt atggtgtctg
       61 ctacggatcg cagatttata aagcacattc agcatggcaa atatttgccg cttcgttgtt
      121 aagattagtc ctggttgatg atttttatat tttaactcca tgatattgat atgtattatt
      181 attgtggtga gttgattgat attgatgcaa tatctcttga gatatatatt cccggttatc
      241 tattgtggga atttaattta agtgcagaag taatattttc gccgtatttt attcggaata
      301 tcctgcttat cctcgtgctg tttctcacgt agtctataat ttccttttta agcccacagg
      361 agagcaacaa tgacaatcca taagaaaggt caggcacact gggaaggcga tatcaaacgc
      421 gggaagggaa cagtatccac ccagagtggc gtgctgaacc aacagccgta tggatttaac
      481 acgcgttttg aaggcgaaaa aggaaccaac cctgaagaac tgattggcgc agcgcatgcc
      541 gcatgtttct caatggcgct ttcattaatg ctgggggaag cgggattcac gccaacatcg
      601 attgatacca ccgccgatgt gtcgctggat aaagtggatg ccgattttgc gattacgaaa
      661 atcgcactga agagtgaagt tgcggtgccg ggtattgatg cctctacctt tgacggcatc
      721 atccagaaag caaaagcagg atgcccggtt tctcaggtac tgaaagcgga aattacgctg
      781 gattaccagt tgaaatcgta aagcattgcc ggatgacgcg tcaggcgcgt gaatgcctga
      841 tgcgttgtta gcatctctac agcgcccgcc agcaccggag ctcacgtccg tcctccctta
      901 ttgctaatga ttgccggact tcacatccgt gggttgccag agggcaacgt tcgtagaaaa
      961 aacagccttg tggcaacgtg cggtttcccg gcagatccgt tttacataat gcccattctt
     1021 cctccagcgg tttatcaatg gcggggaggg aatccagcaa taatcgggtg tatggatgtg
     1081 caggtgccgt cagcacctgc tgcgcgtcgc ccagttctac aatctgcccg agatacatca
     1141 ccgccacccg atcgctcata tgacgtatca ccgagacatt gtgtgaaatc agcacataag
     1201 tcagcccgtg attttcctgt agcgttacca gtaaattgag gatctgcgcc tgcacggaga
     1261 tatccagcgc cgaggttggc tcatcaagca caatcacgtc aggctgcgaa gagagtgctc
     1321 tggcaatggc gatgcgttgc cgctgcccgc cggagaacgc atgaggcagg cggtcgagat
     1381 actccggacg aatacccacc tgcacagcca actcctttgc caacgctcgc cgctgttgtt
     1441 cactactacg cttagctatc cagagcggtt ctgtgatgat gcgccacacc gataagcgcg
     1501 gattaagcga agagagcggg tcctgaaaca ccatctgcat aatgcgttgt gatcctgaac
     1561 ggatgtattg cccgtggctc ggttgcagca tacccattaa aagctgtgcg agggtgcttt
     1621 tgccgcagcc tgactcaccg acgatcccta aggtttcacc acggcggatc tgtaaatcaa
     1681 taccattaat cgcatgaaca tgttccgtag ttttaccaag ccagctttta cgggccggga
     1741 aattgatatg gacgtcgcgt aacgttaata acgtgtcaga tacttatgac ctcctgctgc
     1801 ggataccaac aggcgcagcg ctggttgttg tcaccacacg ctgtcagcgc cgggacgttt
     1861 tcacactgtg cacctgccgc atagcaacga tcgcgaaaag cgcagccgtc aggcaaatgg
     1921 gtgaggtttg gtaccgtccc tggaatggcg ggtaatggct ggcgtggtac tccatgttct
     1981 ggtgcgcatt gcagcaaacc aatggtatac ggatgccggg gatgatggat aacgtctgcc
     2041 gtcacgccgc tttcaattac gcttccggca tacatcacgt aaacgctatc gcacagttgc
     2101 gacaccacgg ccatatcatg gctgatgaac agtaccgcag ttccactggc gcgagcttta
     2161 tgtttaagca aacgcagtac ctgcaactgt accgtgacgt ccagcgccgt agtcggttcg
     2221 tcggcaataa tcaattgcgg ctcgcaggag aatgccagcg caatcattac ccgctggcgc
     2281 ataccacctg aaagctcaaa cggatagcgc gacataactt ccacggcatc cgggatttgc
     2341 atctcttcca gcagggtaat cgctttagct ctggcttccc gacgacttat tggttgatga
     2401 tggcggatca cgtccatcat ctgaagacct attcgacgtg tcggattgag ggcggtcatc
     2461 ggttcctgaa agatcatcgc cactcgtgcg ccgcgccact gacgaagctg tttctcccga
     2521 gcgttaagaa catcgtctcc cagcagtgaa atctgccccc gatgtacgca ataactgccc
     2581 gtcggtagca gacgcataat cagcattgcg gtgactgatt tacctgagcc ggattctccc
     2641 accagaccga caatttcacc gcggttaatc tgcaaggaca cattgttgag cacgtgaaca
     2701 taaccgttaa aaccggggaa actcaaatgc agctgttgaa tgtccagaac gggttgggtc
     2761 atgactgctt tcctcctgct ttcgggtcaa gcagatcgcg aataccatca ccaaagagat
     2821 taaacccgac ggcggtaagc aaaatcgctg cccccggaaa tgcgcaatac caccactgat
     2881 cgagtacata gttgcgacca ttagctacca tcgcgcccca ttcagcactc ggttgttgag
     2941 cacccaggcc aataaatccc aacgtggcgg ccattaaaat cgcgctaccg atatccagcg
     3001 atgcctgcac gatcagcggc ggcagggagt tacgtaaaat atgccagctg atcagatgcc
     3061 aacgcgacgc accaaaggtt ttcgccgcct gaacataggt atactggcgt acaactaatg
     3121 cttgcccccg cgccaggcgc acataaaagg gaattcgcac aatagcaatc gccagcatgg
     3181 cgttaaataa actcggcccg agagcggcag ccagtgccat tgtcagtacc aacgaaggaa
     3241 tcgacagcat aatgtccatg atgcgcatga tgatggcgtc tgcgcgtccg ccaagcacac
     3301 cggatagaca tccgagtagc gaaccaatca tacccgcaat ggcaaccacg actaatccgg
     3361 cgagaattga ttgctgactg ccgaccagta cgcggctgaa cagatcgcgt cccacttcat
     3421 cggtgccaaa ccagtgcgcc gcagaaggcg gcaaaaggcg ggcggttaaa tcaatggcgt
     3481 tgggatcatg cgtcgcaagc cacggtgaaa aaatcatcat caatagcatt aatacaatga
     3541 tgactgcgcc ggtcacggtg agcggactgc ctttcagcat ccataccagt tttgcaccgt
     3601 taaatcgcgt ttgtttttgt gggcgtacgg cggacgtttc ctcgcttagc atcattcacc
     3661 acctccacgt ccgatacgcg gatcaatcca caaatagagc aaatccacca ccaggttgac
     3721 cagcacataa gcaaatgaaa ccacgacggc aaagcccatc actgccggga agtcgagcgc
     3781 ctgtattgat gttactaccc atgcacccat tccgggccag gcaaaaacgg tttcggtgag
     3841 cactgcgcca tacaacaaat cgcccagcgc cagacccaat acggtaatcg atgggatcaa
     3901 cgcattgggt agcgcataac ataaaacgat ataccagccg ggcaagccgc tggcccgggc
     3961 ggtacgaatg tagtcttcac tcaattgttc cagcatcgct gagcggatct ggcgagcgac
     4021 aattcccagg tgaacgaacg ccagcgttaa tgccggtaag atgagatgtt gcaacgcatt
     4081 gaagaagact tcaccgttgc cttcaagcag cgcatcgagc agataaaagc cggtaacgtg
     4141 cgttggtgga tccagccagt catcaagcct tccgccgccg ggaagaattt gcagatgacc
     4201 ataaaacagc acaatgacgc ccagtccaag ccagaacgca ggtgtggaga ttccggtaat
     4261 ggccattatt cgcaccagat gatccagcca gcgatttcgc cagactgcag agagtatgcc
     4321 cagcgggatg ccaatcagga gtgccagcag cagggcacaa aaagccagtt ccagcgtcgc
     4381 cgggaaaaat atacgcaact cttccagcac cggacgcccg gtacgaatgg atgttcccag
     4441 gtcaccatga aacaggtcgc tgaagtagcg gtaaaactgt acatacagtg gctggtccag
     4501 tcccagttgc tggcgaatat tttccacgat agcatcgctg gcgcggtcac ccgccagtaa
     4561 ccgcgccgga tcgccaggga tcagatgcga gataataaac gtaatcacgc agacgcccgc
     4621 caccacgagc accagccccc agcagcgttg gcgtaaaata ctccagaagg tcacgttatt
     4681 tactcatggt attgatattg aagacctgtt ccagcatggg attgaacaca aagcctttca
     4741 cctctttgtt catcgccagt tggtagtttt tctggaacag gtacacataa gcagcgtcat
     4801 caatgacgat tttctgtgcc tgctggtagt cccgggtacg ctgcgtctgg tcggtggtcg
     4861 caagcgcatt gcgcagtaac ttatcgacct cactgttttc atagaacgag cggttacccg
     4921 gcagaccttt tttgtctgac tcaaaccagt aattcataaa catatacggg tcggcaaaat
     4981 ccggactcca gttgccaatc gcaatgtcgt aatcaccttt acccactctg tcgcgcatgg
     5041 tggcgttcgc cagcttttcc agcttcacat tgatgcccag cttgttgaga ctggattgtg
     5101 tcgccagagc aataggctcc cagttcggat cgttatcgga gtagagaaac gtcaggctgg
     5161 tgggtttgct cgtcacttta tcccattcgg ctttggcttt cgtttcgtca tggttgtatt
     5221 gcattgccgt cgcatcgtag ccccacatgc cttccggaat cgggccgcgc atctgttttc
     5281 cgttaccact cagaatgccg ttaaccatgc cctgataatc ggtagaccag gaaatggccc
     5341 gacgcagatc cgcctgatta agaggcgctt tgctgttatt cagatacaga taggtaacgc
     5401 gcagtgacgg atactctgcc acattgactt tattttcctg cttcagggcg ttgagttgat
     5461 ccaccggcag cgcatcggca atgtcaatgt cgccacggga gagctgcagg cgacgggagg
     5521 cactttcacc gataattttt accgatactc gcttaaagtt cggtttattg ccggggtaat
     5581 gcggatttgg caccagaact aattgctgac ctttttgccc gcttttcagc ataaatggtc
     5641 cggaaccggc ggtattttgc gcgaggaagc cgcgagcatc atccgctgca tgttccttta
     5701 agaccgccgg attgataatg gatgcaccgt cattcgctag cgtgtagagg aacggtgcga
     5761 atggttggct aagggtaaac ttcaccgtat gttcgtcggg agtatcaatc tttaaatctt
     5821 tgggaaatgc ttctgctggc ccctggccga tttttagtaa ccgctcaaaa gagagtttta
     5881 ctgcttcggc agtgacaggt gtgccatcgg caaatttagc attgtccttc agggtgaacg
     5941 tccactcttt ttgatcgtca gacgctttcc agctacttgc cagatcgcct tcaacgtcgg
     6001 ttgagccttt atcaccgtcc gttttgtact gaaccagccg ctgataagac gggtaggtca
     6061 ctgtccagtc gttattatct attgttaccg ccgggtcgag ggtttgtgga tcggcggcct
     6121 taccaatcac cagcatatct ttcggtacgg cggcgtgcgc aaccgggaaa gttgtggcaa
     6181 ggacgagcgc gagcaatgtg ggacgaaacg atatcgatct cttcatgaca gtttcctgat
     6241 taactgacgt gctgtgtgcc aggggatata aaacaagaga attgatcagc gagcagaggg
     6301 taactcgctg cctggggtaa ttcgaagtgc caccattcgc tggagatacc gacaaaaccg
     6361 ccaccagtca ttatcgcatt cagcaacagg cgattgcgct gagcggcggg cgggacggaa
     6421 gggtgatagg catgggagcg ctcgtgcatt tcatcgaacc ctgcgcccat atccaggatg
     6481 ttcccgtgct catcacgaag cgtcaggtcg atcgccgtgc cacggctgtg attagaaccg
     6541 accgtcacat caacaacata ttgcgggtct gggcaggctt gccacaacat cgcctgtgct
     6601 tgttgtgggc gatacgcatc gtaaatcacc agttgtaagc ctgacagctg ggcgatactg
     6661 atacttttcg ccagcgcggt aatcgcatcc ttgtgtaaca gacaacgcgc ttgctggtaa
     6721 atagctttac ctgtgatgtt atcagcgcag gcgtatttca attcgatctc cagatcaggg
     6781 aagatcacgg ctaaatcaac cagttcggtg gtatccgaca taaacggttc ctgtattaag
     6841 acaaaaattg ctgcgctttc cgcttatgca gatctcatgc catgccggga taagcgccag
     6901 aatgctggct taaagttata ttttttaact tttgatcaac atttgtgcag cgtagtgcag
     6961 ttttggtgca agaggggaag ttaaggaagg aatctcccgg aatcgtagct gaaatcacag
     7021 tatttaagtg acagcgtcac gttaaatgaa aacccgcgag tgcgggcgag aggaatttgt
     7081 cagattttca gcggtaacac gctgctcatc cagcctggaa tttcctcggc gggtaggggg
     7141 cgggaaaaga aatatccctg aataacgcga cagtggatct tgcgtagcat ctcaaattgc
     7201 tctttggttt cgacgccttc cgccacgacg gttaaattga ggctttgccc aatgctggta
     7261 atggcttcaa gtaaggcaag gatgcgtttt tccgtcagac aacgatcgac aaaacttttg
     7321 tcaattttga tttccgttac cggaagactg actaagcggg ataatccgga aaagcccgtg
     7381 ccaaaatcat ctaccgataa gcccacgccc atatcgcgca ggatctgaat gcgcttaaag
     7441 atttcggtat cgtgttccat catcatgctt tccgtgattt ctaccgtcag ctggtggccg
     7501 tcaatacccc aggcttgcat tgcatcagac acctgattag gcagctgatt actgcgaaag
     7561 tgcagcgccg acaagttcac ggataacgcc gggatatgaa tattctggct acgccattct
     7621 gctaactgac ggcaagcttc cgcgatgacc cagcgcccaa tattttcgat ttcaccaatc
     7681 tcttctgcga gaggaataaa ccgtgaaggg ggtacatgac catgctgggg atcgtgccag
     7741 cgagcaaggg cttcgatgcc gtacagttca cccgtttctg cgaagatttg cggctggtaa
     7801 accagtttca gttggttatt gctaatcgct tctttcagcg ctgcgcctaa aaccaaacgc
     7861 tcttttacca tttcgttcat cgccgggctg aagaactgcc agccgttacc gccattcttg
     7921 cgaatataat ccattgcatt gtgagcagtg gagagcaggt aatcgcggtt tttacccacg
     7981 tcgtagctga tgccaatact caaggttagc gggaatggtt tatcgtcaat cattatcggc
     8041 ttgctgacca cattccgtag ctcatcggcg atttgggtaa tgttactgac gtcgttttcc
     8101 aggctcacga ggacaaactg cgtaccttcg atacgacaga gatactgatc cggtttgagt
     8161 ttttcacgaa agcgattgac cacttccagc aatgcctgat cggcccacgc atagccaagg
     8221 ctatcaatca catcctgaat atggtcaaca ccgatgagat acaccaccgg agagacggct
     8281 ttgtcgacaa ggtcatcgag gtaattgtgc aggttattgc gatttggcag accggtcatc
     8341 ggatcaaatt ggatgagttg ttcaatatgc tgacggcttt tttcctgttc cagcgccagc
     8401 gcggccatat gctggctgat atctgccacg cgttcgataa aggcgctggt ttctgctcct
     8461 gacgaggttt taatttgcag gatccccgca ggcgcaccat cacgctgacg aatggtcgct
     8521 gaccagcttt gcgcattttg aacttctgca ccgtgcgaag atgacgccca gtgtatcggc
     8581 atcccgttgc gcagtgcgaa cagcgaaaca tgcgattcgt tgagaacaga ttcgatgtta
     8641 cgacaaatga tttcccccat ttcatgaaat ggcgggctgc tgcacatggc ggcgagaata
     8701 ttgccttcaa gctggcggat ctgccgttct tcggtgatat ccgagaaggt cattaccagg
     8761 ttctgcagat gcgcgagcac gtcataaacc gggctgatag aggctttaat ccagattttt
     8821 tcaccggtgc gtgtcaacag cagaaattcg tcctgatcgc gggcggtttt ccatagcaac
     8881 tgttgtaaac gaatgcggtt atcggcaggg aattcaggaa tgttcaggag tgtatcgggc
     8941 tgcataccgc tggcttcgct aatgcagtaa ccaaacattt cggtaaatgc gcgattgcac
     9001 tgcacaatat ggcgttccgg atcgaggaca atcaccggtc ggtcgagatg gtcaacggca
     9061 ataatcaatt gtcgggtctg ttctttttgc gccatttcta cgctggcatc ccgtaccagc
     9121 gccaggtaat aaactttccc ctcggcgctc actttcgata gcgcaaaacg ggtccagatt
     9181 ttactgccgt cttttttctc cagctgcagc tcccgactca tcccctcgac gcgcgcttta
     9241 ccaccttcac ggttatgacg aatgtattca ggatgcgcag gacgcaaatc ccgtggaatc
     9301 agcatatcaa tgttattgcc aatgacttct tcacgtttgt acccccagag cttctctgcg
     9361 gcggggttga aaaacatcac ttcatcattt tcgttaatta acaccgc
//
LOCUS       DAZOYL010000150         9307 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZOYL010000150 DAZOYL010000000
VERSION     DAZOYL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.150"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(10..636)
                     /gene="upp"
                     /locus_tag="ACHUOY_002606"
     CDS             complement(10..636)
                     /gene="upp"
                     /locus_tag="ACHUOY_002606"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR0683354.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            961..1998
                     /gene="purM"
                     /locus_tag="ACHUOY_002607"
     CDS             961..1998
                     /gene="purM"
                     /locus_tag="ACHUOY_002607"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR0683355.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            1998..2636
                     /gene="purN"
                     /locus_tag="ACHUOY_002608"
     CDS             1998..2636
                     /gene="purN"
                     /locus_tag="ACHUOY_002608"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR0683356.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2807..4873
                     /gene="ppk1"
                     /locus_tag="ACHUOY_002609"
     CDS             2807..4873
                     /gene="ppk1"
                     /locus_tag="ACHUOY_002609"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR0683357.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4878..6419
                     /gene="ppx"
                     /locus_tag="ACHUOY_002610"
     CDS             4878..6419
                     /gene="ppx"
                     /locus_tag="ACHUOY_002610"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR0683358.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6458..8701)
                     /gene="pdeF"
                     /locus_tag="ACHUOY_002611"
     CDS             complement(6458..8701)
                     /gene="pdeF"
                     /locus_tag="ACHUOY_002611"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0683359.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(8883..9035)
                     /locus_tag="ACHUOY_002612"
     CDS             complement(8883..9035)
                     /locus_tag="ACHUOY_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683360.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            9053..9244
                     /locus_tag="ACHUOY_002613"
     CDS             9053..9244
                     /locus_tag="ACHUOY_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR0683361.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT         2417 a         2286 c         2267 g         2337 t
ORIGIN      
        1 ttttattctt tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat
       61 gtatccgtgc tcgttcagtc cctggtcaat cgatgcggta tacagttcga cgtccgggtg
      121 cgctttttcc agcgcagcga taccttctgg cgcagctacc agcaccagaa ctttgatgct
      181 gctgcagccc gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag
      241 cattgggtca acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta
      301 cggtaccggc tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct
      361 cggaacattt tccagcacac cgtccatcat ccccagaccg gcacgcagaa ttggcacaac
      421 ggtaattttc ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt
      481 tactttttcc gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga
      541 agcgagttcg cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg
      601 tttgacgagt gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc
      661 cacaaaaaaa atcgacggat tatacctcct ttcttcaagg cggcaatatt cttttcgttg
      721 actttagtca aaatgataac ggtttgagat aaagttattt tatattcaaa tggttatgaa
      781 agaagattat tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc
      841 gtctggggaa aagacgtgca aaaaggttgt gcaaagcagt ctcgaaaacg tttgctttcc
      901 ctgttagaat tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca
      961 gtgaccgata aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat
     1021 gctctggttg gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc
     1081 ggtctgggcg gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg
     1141 gtttccggca ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac
     1201 gacaccattg gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgca
     1261 gaaccgctgt ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca
     1321 gcggtgatca gcggtatcgc ggaaggatgt ctgcaatcgg gctgttcact ggtgggtggc
     1381 gaaacggcag aaatgccggg gatgtatcac ggtgaggatt acgatgtcgc gggtttctgc
     1441 gttggcgtgg tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg
     1501 ctgattgcac tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt
     1561 cttgaagtca gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat
     1621 catctgctgg caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc
     1681 gatgtgcatg ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta
     1741 ttgccagata atactcaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc
     1801 aactggctgc aaacggctgg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc
     1861 ggcgtcggga tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta
     1921 aatgccaacg gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa
     1981 cgcgtggtta tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac
     2041 aggcaattat tgacgcctgt aaaaccaaca aaattaaagg caccatacgg gcagttttca
     2101 gcaataaggc cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata
     2161 cgctcatcgc cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa
     2221 tcgacatgta cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg
     2281 cgtttgtctc ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat
     2341 atcccggatt acacacccat cgtcaggcgc tggaaaatgg tgatgaagag cacggtacat
     2401 cggtgcattt cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc
     2461 cggtatttgc tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg
     2521 ctatttatcc actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg
     2581 ccgcgtggct ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc
     2641 ccgtagttaa agcgccagct ctgccgctgg cgtttttcaa ttcacctgta aatcgcaagc
     2701 tccagcagtt tttttccccc ttttctggca tagttggaca tctgccaata ttgctcgcca
     2761 taatatccag gcagtgtccc gtgaataaaa cggagtaaaa gtggtaatgg gtcaggaaaa
     2821 gctatacatc gaaaaagagc tcagttggtt atcgttcaat gaacgcgtgc ttcaggaagc
     2881 ggcggacaaa tctaacccgc tgattgaaag gatgcgtttc ctggggatct attccaataa
     2941 ccttgatgag ttctataaag tccgcttcgc tgaactgaag cgacgcatca ttattagcga
     3001 agaacaaggc tccaactctc attcccgcca tttactgggc aaaattcagt cccgggtgct
     3061 gaaagccgat caggaattcg acggcctcta caacgagctg ctgctggaga tggcgcgtaa
     3121 ccagatcttc ctgattaatg aacgccagct ctccgtcaat caacaaaact ggctgcgtca
     3181 ttattttaag cagtatctgc gtcagcacat tacgccgatt ttaatcaatc ctgacactga
     3241 cttagtgcag ttcctgaaag atgattacac ctatctggcg gtggaaatta tccgtggcga
     3301 taccatccgt tacgcgctgc tggagatccc atcagataaa gtgccgcgct ttgtgaattt
     3361 accgccagaa gcaccgcgtc gacgcaagcc gatgattctt ctggataaca ttctgcgtta
     3421 ctgccttgat gatattttca aaggcttctt tgattatgac gcgctgaatg cctattcaat
     3481 gaagatgacc cgcgatgccg aatacgattt agtgcatgag atggaagcca gcctgatgga
     3541 gttgatgtct tccagtctca agcagcgttt aactgctgag ccggtgcgtt ttgtttatca
     3601 gcgcgatatg cccaatgcgc tggttgaagt gttacgcgaa aaactgacta tttcccgcta
     3661 cgactccatc gtccccggcg gtcgttatca taattttaaa gactttatta atttccccaa
     3721 tgtcggcaaa gccaatctgg tgaacaaacc actgccgcgt ttacgccata tttggtttga
     3781 taaagcccag ttccgcaatg gttttgatgc cattcgcgaa cgcgatgtgt tgctctatta
     3841 tccttatcac acctttgagc atgtgctgga actgctgcgt caggcttcgt tcgacccgag
     3901 cgtactggcg attaaaatta acatttaccg tgtggcaaaa gattcacgta tcatcgactc
     3961 gatgatccac gctgcgcaca acggcaagaa agtgaccgtg gtggttgagt tacaggcgcg
     4021 tttcgacgaa gaagccaaca ttcactgggc gaagcgcctg accgaagcag gcgtgcacgt
     4081 tatcttctct gcgccggggc tgaaaattca cgccaaactg ttcctgattt cacgtaaaga
     4141 aaacggtgaa gtggtccgtt acgcacacat cgggaccggg aactttaacg aaaaaaccgc
     4201 gcgtctttat actgactatt cgttgctgac cgcagatgcg cgtatcacca acgaagtacg
     4261 gcgggtattt aactttattg aaaacccata ccgcccggtg acatttgatt atttaatggt
     4321 gtcaccgcaa aactctcgcc gtctgttata tgaaatggta gaccgcgaaa tcgccaacgc
     4381 gcagcaaggg ctgcccagtg gtatcaccct gaagctaaat aaccttgtcg ataaaggcct
     4441 ggttgatcgt ctgtatgtgg cctccagctc cggcgtaccg gttaatctgc tggttcgcgg
     4501 aatgtgttcg ctgatcccca atctggaagg cattagcgac aacattcgtg ccatcagtat
     4561 tgttgaccgt taccttgaac atgaccgggt ttatattttt gaaaatggcg gcgataaaaa
     4621 ggtctacctt tcttccgccg actggatgac gcgcaatatt gattatcgta ttgaagtggc
     4681 gacaccgctg ctcgatccgc gcctgaagca gcgggtgctg gacatcatcg acatattgtt
     4741 cagcgatacg gtcaaagctc gttatatcga taaagaactc agtaatcgct acgttccccg
     4801 cggcaatcgc cgcaaagtac gggcgcagtt ggcgatttat gactacatca aatcactcga
     4861 acaacctgaa taaccctatg ccaatacacg ataaatcccc tcgtccgcag gagtttgctg
     4921 cggtcgatct tggttcaaac agttttcaca tggtcatagc ccgtgtggta gatggtgcca
     4981 tgcagattat tggccgcctg aaacagcggg tgcatctggc ggacggcctg gggccagata
     5041 atatgttgag tgaagaggca atgacgcgcg gtttgaactg tctgtcgctg tttgccgaac
     5101 ggctacaagg gttttctcct gccagcgtct gtatagtcgg cacccatacg ctgcgtcagg
     5161 cgctgaacgc cactgacttt ctgaaacgcg cggaaaaggt cattccctac ccgattgaaa
     5221 ttatttccgg taatgaagaa gcccgtctga tttttatggg cgtggaacat acccaaccgg
     5281 agaaaggtcg caaactggtt attgatattg gcggcggatc tacggaactg gtgattggtg
     5341 aaaatttcga accgattctc gttgaaagcc gccggatggg ttgtgtcagc tttgcccagc
     5401 tttacttccc tggcggggtc atcaataaag agaattttca gcgcgctcgt atggcggcag
     5461 cacaaaaact ggaaacttta acctggcaat tccgtattca gggctggaac gtggcaatgg
     5521 gcgcttccgg taccataaaa gccgcccatg aagtgttaat ggaaatgggc gagaaagacg
     5581 ggataattac cccggaacgt ctggaaaaac tggtaaaaga agttttacgt caccgtaatt
     5641 tcgcatcgct gagtttaccg ggtctttccg aagagcggaa aacagtcttc gttcctggac
     5701 tggcgatttt atgcggtgtg tttgatgctt tagccatccg tgaactgcgc ctttctgacg
     5761 gggcgttacg cgaaggcgta ctgtacgaaa tggaaggacg tttccgtcat caggatgtgc
     5821 gtagtcgcac cgccagcagc ctcgccaacc agtatcacat cgacagcgaa caggcccgac
     5881 gggtgctgga taccactatg caaatgtacg aacagtggcg ggaacagcaa ccgaagctgg
     5941 cgcatccgca actggaggcg ctactgcgat gggccgccat gctgcatgag gtcgggttga
     6001 atatcaacca cagcggtttg catcgccact ccgcttatat tctgcaaaac agtgacttgc
     6061 cgggttttaa tcaggaacag cagctgatga tggcgacact ggtgcgctat caccgtaaag
     6121 cgattaaact cgacgatctg ccgcgcttta ccttgtttaa gaagaaacag ttcctgccac
     6181 tgattcagct attgcgcctt ggcgtattac tcaacaatca acgtcaggca accaccacac
     6241 cgccaacatt gacactgatt actgatgaca gtcactggac actgcgtttc ccgcatgact
     6301 ggtttagtca gaatgcgttg gtactgcttg atctggaaaa ggagcaagaa tactgggaag
     6361 gcgtggctgg ctggcggttg aaaattgaag aagaaagtac acctgaaatc gccgcttaat
     6421 gctttgcggg ccgacgagaa atgtcggccc gcattattca ggcactttcg cgaatgggtt
     6481 cgatttcatt caacgtatca attaacggct gcggcttacc aataagataa ccctgcatat
     6541 aatcgatccc caaagagagc accgcctcgc ggatctcttc gttttcaacg tactctgcca
     6601 ctacccgcat tttcttcatt cgcgccagat ggcaaatcga cgccacaatc tgataatcca
     6661 gactatttga cacaatattg cggataaagc tgccgtcaat tttaagcaga tcggcattca
     6721 catttttaag ccgtgcatag ctggcgtagc cggtgccgaa atcatcaatc gcaatctggc
     6781 agcctaattc ctgaagatgc tgcaaggtga tttgcgcctg cttaacattg gtcagagcat
     6841 tactttcggt gacttcaaaa ataagttgcc acgcttcaat ctgatattta gccagcaact
     6901 gactgacttc aacaggaaaa cgagcctgac ataccgaggt tggagacaga ttaatagcaa
     6961 aacggtgagc gggcatcttc gctctgtttt cagccataaa ttgcagcgta tgctcaatga
     7021 cccacatgtc gatactcgac gataaaccaa attcgtgcgc gaccggcaag aagctatcgg
     7081 ggacgatcag ttcatcattc tcacctttca tgcgcagaag aatttcatgg taaacatcac
     7141 cacgcatacc ggtaatcggc tgggccatca ggaaaaaatg gttgtgttcc agcgcctgct
     7201 gtagtcgatt catcatcgcg actttatctt tcaattcgcg ttgcaaatac attgccccgc
     7261 gacgctgcat attttccggg gcgttggtca cgatggaaag ttcggcgacc gtatttagct
     7321 ctcccagcag caggtagata tgattcactg gcgagcgcac atagcagtaa ctgacgccaa
     7381 tctgcggttg catcggcatc ccatcccaaa agaaacgaaa ttgcttgaga tgactatcca
     7441 gtgcggtaat gcgctcctgg tgcgattctg tattcagtcg cagcgcgaga tcgttacccg
     7501 aaagctgata aacatcttca cccggttcca gcaagggtga cagccagtga gaaagttttt
     7561 gcttgtattg aatccgcagc atgatgccat agttcttaac cagcatttcc atgccaggga
     7621 tgcgtaaata acaaagcgca gaccagggag catcacgcaa cgcgcgattc agggcgcgaa
     7681 catttggcag atgaaccacc gggtccatat acgcaagccg ctgaatgcgt ctgactaccg
     7741 ctctctgacg ggttgccagt acagccatat aattgacaat aaaagagaat accagataac
     7801 tggaggaggt tatggtcagc tgcgtggtat agccaggata aatgggaatg taattttgat
     7861 agctgtggat gctgatcatc aacacgaccg cccagagcag cgaaatcagc ttataaccat
     7921 agcgcatcgc tccccacatc atcaggggca gcaataatga caaggtataa ttggtgctaa
     7981 aaattgtgct tttttcattt aacggcatgc ataacagcaa caataaagca cctaatgcca
     8041 gtagccagag cgcgaactct tttttggtga ctttggcatc aacctgctgt tttaattgcg
     8101 aataatagct acgtaaataa aatggatttc gcactacccg aatgatgaag tagcacagcg
     8161 ggacaccgat cagattaccc accagtaagg cctgataatt gattaaagtc ccgaggttaa
     8221 aaggcatgac accgaccaga ttttctctgc tcgccagtaa tcctacaaat gcagcaaact
     8281 ggaaaagtat cagaaacagc gttgcaggaa acacaatctg ccagaatata cgctgggaaa
     8341 ttaaacgggt atcaccatgt gaaacgttgt tacgccgggg agtaaagacc ctgtaaccgc
     8401 cccaacagag aatgataatg ataataaaat tagccgttaa tgatagcgtt tcgtaaaaac
     8461 ccagctctgc atatttgcgg acaaaaatcc ctaaggcgat ccctggcaat gccgcccagc
     8521 tgaaaaacat catcatgctg atcatcagtg ccagaggcag ataaaagaga aaaacctcac
     8581 cggaagaaat atgcgcgaaa gtattaatgt gggcgaaaat gggcaagatt aaagaaggca
     8641 ggaacagcgg aagcccccac catttatcac gcatttttat ataagttgca ttcagtttca
     8701 tagatgctca gcagaatccc ccacatcctg aaggaggtgt attcagacag gcatcccacc
     8761 tgacttcgaa tgatgattat tcatcactat agagagcatt gattctaagt gtcatatgaa
     8821 agttccaatt gatatatatc aaacaaaata accctgatta atgaattatt acgtttatca
     8881 tgttaattca tcattattac atcatcattg taaataatta aattaacttc cataacatta
     8941 aaatatgtat ccactgacgt ttttttacat aacgaagaat tgaccatttt gtcctgttgt
     9001 gccttaatgt aagtaccgtc cacagcgtgg gacatacttc aaggaacctt ttgtgagtca
     9061 ggcaaccagt atgcgaaaac gacaccgatt taacagtcgc atgacccgta tcgtactgct
     9121 catcagcttt atcttcttct ttggccgttt tatctactcg tccgtcggtg cctggcagca
     9181 ccatcagagc aaaaaagaag ctcagcaatc cacactctcc gtcgaatcac cggtacaacg
     9241 ttagcggtta ccttcttcac tttcacagaa cataacggca cttcgctgtc ggatgctttt
     9301 gctcttt
//
LOCUS       DAZOYL010000151         9188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZOYL010000151 DAZOYL010000000
VERSION     DAZOYL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.151"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUOY_002614"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUOY_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHUOY_002615"
     CDS             complement(1751..2509)
                     /locus_tag="ACHUOY_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR0683362.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUOY_002616"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUOY_002616"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR0683363.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUOY_002617"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUOY_002617"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR0683364.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUOY_002618"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUOY_002618"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR0683365.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHUOY_002619"
     CDS             complement(5474..6079)
                     /locus_tag="ACHUOY_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683366.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="ACHUOY_002620"
     CDS             complement(6082..6849)
                     /locus_tag="ACHUOY_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683367.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="ACHUOY_002621"
     CDS             complement(6852..7562)
                     /locus_tag="ACHUOY_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683368.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="ACHUOY_002622"
     CDS             complement(7578..9083)
                     /locus_tag="ACHUOY_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0683369.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2357 c         2262 g         2197 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac tctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctttgg
//
LOCUS       DAZOYL010000152         9098 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZOYL010000152 DAZOYL010000000
VERSION     DAZOYL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.152"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1407)
                     /locus_tag="ACHUOY_002623"
     CDS             complement(<1..1407)
                     /locus_tag="ACHUOY_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR0683370.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDR"
     gene            1737..3035
                     /gene="dadA"
                     /locus_tag="ACHUOY_002624"
     CDS             1737..3035
                     /gene="dadA"
                     /locus_tag="ACHUOY_002624"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR0683371.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            3045..4115
                     /gene="dadX"
                     /locus_tag="ACHUOY_002625"
     CDS             3045..4115
                     /gene="dadX"
                     /locus_tag="ACHUOY_002625"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR0683372.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(4502..6238)
                     /locus_tag="ACHUOY_002626"
     CDS             complement(4502..6238)
                     /locus_tag="ACHUOY_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR0683373.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(6333..7247)
                     /gene="ldcA"
                     /locus_tag="ACHUOY_002627"
     CDS             complement(6333..7247)
                     /gene="ldcA"
                     /locus_tag="ACHUOY_002627"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR0683374.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            7347..7958
                     /gene="emtA"
                     /locus_tag="ACHUOY_002628"
     CDS             7347..7958
                     /gene="emtA"
                     /locus_tag="ACHUOY_002628"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR0683375.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(7960..8694)
                     /locus_tag="ACHUOY_002629"
     CDS             complement(7960..8694)
                     /locus_tag="ACHUOY_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR0683376.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            8895..>9098
                     /locus_tag="ACHUOY_002630"
     CDS             8895..>9098
                     /locus_tag="ACHUOY_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0683377.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         2233 a         2312 c         2419 g         2134 t
ORIGIN      
        1 ccgatccagc ggtgcgcgat tatgtggaat ataacgcagc gtcagcgaac ggtcgccgcg
       61 caaatccacg ttccagatct gaatattcgg ctccagatta cttaagttat attgcgacga
      121 taaccggtta cgcatctccc gataaccttc ttcattatga atagcggaaa tctccagata
      181 attatgccga tcatcatcca gcacggtgaa gaagcggaaa tcacgcatca ctttcggtga
      241 cagaaactgg ctgataaaac tttcatcttt gaaatcacgc atcgcgaaat gcagcgtttc
      301 cagccagtcg gaaccggcga tatctgggaa ccagtatttg tcttcttccg ttggcgactg
      361 acaaatccgt ttaatatcct ggaacatggc gaacccgagg gcatacgggt tgatgccgct
      421 gtaccacggg ctgttatagg ggggctggaa gaccacattg gtgtggctgt gcaaaaactc
      481 cagcataaaa cgttccgtta ctttcccttc atcatacaga tggttaagga tggtgtagtg
      541 ccagaaggtc gcccagcctt cgttcatcac ctgagtctgt ttttgcggat aaaaatactg
      601 gctcacctta cgcacaatac gcaggatttc acgctgccat gattccagca gtggcgcatt
      661 tttctccata aaatagagca ggttttcttg tggttcggac ggatagcggc gcgcttcagc
      721 aaccgttttc tcttcctcgc gcttcggcag agtacgccag agcatattga cctgactttg
      781 cagatactct tcgcgacttt tctgccgggc tttctcttct tgcagcgaga ttttttgcgg
      841 gcgtttgtac cggtccacgc cgtagttcat cagcgcgtgg cacgagtcca gaagccgttc
      901 tacttcatcc acgccataac gttcttcgca ctcggtaata tatttacggg caaaaatcag
      961 ataatcgaca atcgaactgg cgtcggtcca actgcggaat aagtaattgt ttttaaagaa
     1021 agagttatgc ccatagcagg cgtgtgccat caccagcgct tgcatggtaa tggtgttctc
     1081 ttccatcagg taagcgatac acgggttaga gttaatgacg atttcatagg ccagcccttg
     1141 ctgaccgtgc ttatacagcc gttcagtctc gataaatttt ttaccgaatg accagtgcgg
     1201 atagttaatt ggcatgccga cgctggagta ggcatccatc atctgttctg aggttatcac
     1261 ttcaatctgg tgcgggtagg tatccagccg gtagagtttc gccacccggt ctatctctgc
     1321 cagataaaca tccagcaggt cgaacgtcca gtcgggtcca tcgctcaaac gtgtggtgtc
     1381 cttattcata gaatcgatcg tcgccatacg cgcacctcat tgttgtcggc gctctctgtg
     1441 tggagcacct catttcaagc atagaacacc tgttaaaaac cgcgtcgccg gagaattttt
     1501 ttctttgcga tttcttatta tcagagtgcc actaatccgc ttctgaacgg aattttatgc
     1561 tggataaaaa gggcgttcag caggagatac taaagacgcc atattgccgc agagtcaggg
     1621 agatgtgagc cagctcacca taaaaaagct gtatgttgaa taatgttttc aactgagtta
     1681 tcaagatgta attagattat tattctttta ctgtatctac cgttatcgga gtggctatgc
     1741 gagttgtcat actgggaagt ggtgtggtag gcgttgccag cgcctggtac ttaaatcagg
     1801 caggacatga ggtcaccgtc attgatcggg agccgggggc cgccctggaa accagcgccg
     1861 cgaatgccgg gcaaatctcc cccggatatg ctgcaccgtg ggcggcacca ggtgtgcctt
     1921 taaaagcgat caaatggatg ttccagcgcc atgcgccgct ggcggttcgt ctcgacggta
     1981 cgcagtttca gttgaaatgg atgtggcaaa tgttacgtaa ctgcgacacc agccactaca
     2041 tggaaaacaa agggcggatg gtgcgtctgg cggaatacag ccgtgattgc ctgaaagcat
     2101 tgcgcgccga aaccaatatt cagtatgaag ggcgtcaggg tgggacgctg caactgttcc
     2161 gtaccgaaca acagtatgaa aatgcgaccc gcgatatcgc cgtgctggaa gatgccggcg
     2221 taccgtatca gctgctggaa tccagccgcc tggcggaagt ggagcccgcg ctggcagaag
     2281 tagcgcacaa actgacgggt ggtctgcaac tgcccaatga tgaaaccgga gactgccagc
     2341 tgtttaccca gaatctggcg cggatggcag agcaggcggg ggttaaattc cgctttaata
     2401 cgcccgttga ccaactgctt tgcgacggcg agcaaatcta cggcgtgaag tgtggcgatg
     2461 aagtgattaa ggccgatgcg tatgtgatgg cgtttggttc ttactcgacg gcgatgctca
     2521 aaggcattgt tgatattccg gtgtatccgc tgaaaggcta ctcgctgact attccaattg
     2581 cgcaggaaga tggtgcgcca gtatccacca ttcttgatga aacctacaaa atcgccatta
     2641 cccgtttcga taaccgcatt cgtgttggcg gaatggcgga aattgttggt tttaacaccg
     2701 agctgttgca accgcgtcgt gaaacgctgg aaatggtggt tcgcgatctc tatccacgcg
     2761 gcggtcatgt cgagcaggcg actttctgga cggggttgcg cccgatgacg ccagacggta
     2821 cgccggttgt cgggcgtaca cgctttaaaa atctgtggct gaataccggt cacggcacgc
     2881 tcggctggac gatggcttgc ggttccggtc agttgttaag cgatctgctc tctggtcgca
     2941 cgccagcgat cccatatgag gatctaagcg tagcgcgcta cagccgtgga tttacgccat
     3001 cacgtccggg ccatttacat ggcgcacaca gctaaggaaa cgagatgacc cgtccgatac
     3061 aggccagcct cgatctgcag gcattaaaac agaatctgtc cattgtccgc caggccgcgc
     3121 cgcacgcgcg cgtctggtcg gtggtaaaag cgaacgctta cgggcatggt attgagcgta
     3181 tctggagcgc gctcggggcc accgatggct ttgcattact taacctggaa gaggcaataa
     3241 cgttacgtga gcgcggctgg aaggggccga tcctgatgct ggaaggattt ttccatgctc
     3301 aggatctgga gatttatgac cagcaccgcc tgaccacctg cgtacacagc aactggcagc
     3361 tcaaagcact gcaaaatgcg cggctaaaag caccgttgga tatttatctt aaagtgaaca
     3421 gtgggatgaa tcggttgggc ttccagcccg atcgcgtgct taccgtctgg cagcagttgc
     3481 gggcaatggc gaatgttggc gagatgaccc tgatgtcgca ttttgccgaa gcggaacatc
     3541 ctgatggaat ttccagcgcg atggcgcgta ttgagcaggc ggcggaaggg ctggagtgtc
     3601 ggcgttcgtt gtccaattcg gcggcgactc tgtggcaccc ggaagcgcat tttgactggg
     3661 ttcggcctgg cattattttg tatggcgctt cgccgtccgg tcagtggcgt gatatcgcca
     3721 ataccggatt acgtccggtg atgacgctaa gcagtgagat tattggtgtc cagacgctaa
     3781 aagcgggtga gcgtgtgggc tacggcggtc gctatactgc gcgcgatgaa cagcgaatcg
     3841 gcattgtcgc cgcagggtac gccgacggtt atccgcgcca cgcgcctacc ggtacccctg
     3901 ttttagtgga cggcgtgcgc accatgacgg tggggaccgt ctcgatggat atgctagcgg
     3961 tcgatttaac gccttgcccg caggcgggta ttggtacgcc ggttgagctg tggggcaagg
     4021 agatcaaaat tgatgatgtc gccgccgctg ccggaacggt gggctatgag ttgatgtgcg
     4081 cgctggtgct acgcgtcccg gttgtgacgg tgtaacttgt tgtaagccgg atcggaggca
     4141 acgtcttctg ggtgcaaaaa aaatcatcca tccggctggt cagcaactgt agttgttaat
     4201 gtgacagagc cattgcccat gatagtgtcc attaaaagga tggacactat ttccccggaa
     4261 cctgaactca ccgcacaggc gttctacata aaacgcttac gcttcattgt tgactcgaac
     4321 tcgacttcag ataaatcacg ctttaccctt gatggagcct gtacatagat ttgtgtaatt
     4381 gcctgatttt gatatgttca attcaacatc aaatgaaggt taaattatgg acgacaaaca
     4441 attgcaggct caggctgcgt tcagcaaagc atcgcaaccg gcgatagatg cttcattaaa
     4501 tttaagattc agcttcctct tcagccaccc gtacgccaat cttcaacact tcattatctt
     4561 ctttctcggc caccgtccag atcatcccgg caaactctac ctggtcaccg acaaccggtg
     4621 ccgcgcctaa caactgctgg acaatttcac ccagcgtttg ctgcttatca cgatattctc
     4681 gcccgtcttc caggccatat atcagcgcaa catcagcata tttggcgctg gcttcgagaa
     4741 tgaagtcacc aaagaagcgt tgatccagcg cgaccggtgg cgactggctg aacagtttac
     4801 cgagcgccgg gagatcgcgt tcccgaccaa ttacacacaa cacatcgcct tcgcgcagtc
     4861 gggtgctgcc ggtaggatga agcaactggt tatcacgaaa tagtgccgca atacgcgtct
     4921 cttttggcat atgcagatcg cgcagtgccg cacctacaca ccatttatcg gcactcaatt
     4981 gataaacaaa ctgctcccac ggattttccg gatgaatatc cagaccaacg cgtgacactg
     5041 gacgtccgac gggcggaacc accactttgg cttttttcgc cgcccacgag agtgatgttc
     5101 cctgcaagag cagtgaaacc agaaccacaa agaaggcgac attaaagaac agacgtgcat
     5161 tctccagccc cgccatcatc gggaacacgg ccaggatgat tggcaccgcg ccgcgtaatc
     5221 ctacccagct gataaacacg cgctcacgca gattgaaacc gcggaagggg agcaatccag
     5281 caaataccga aaggggacgg gcgaagaata tcatccatgc cgacaaaatg agcgccggaa
     5341 tggcaatagg cagcagatcg cttgggttaa ccaatagccc cagcaccagg aacatggcga
     5401 tttgcgccag ccaggcgagg ccgtcgaaat tttgcaggat gccgtagcgg ttgcgaatcg
     5461 gccgattacc cagcagaaaa ccgcacagat aaacagccag aataccgctg ccttccagcg
     5521 ccgtggttaa agcaaaaatt aaaataccgc cgcttaatgc cagcaatgga tataatccgg
     5581 caggcagggc gatgcgatta atcatttgca gcagtaaata accaccgcca agtccgatga
     5641 caatcccgag gccaaattgc tgcagaatat cgaccacgaa catccaactg acactgcttt
     5701 catgttgctg gatcatcgca atcagggtaa tcgtcagaaa gaccgccatt ggatcattac
     5761 tgccggattc aatttccagc gtcgagccaa cacgttcgtt aagcccctta ccacccagca
     5821 gagaaaagac cgctgcagca tcggtagagc cgacgatagc gccgattaat aagccttcaa
     5881 tcaaatcaag attaaacagc cacgccgcca tcatgccggt taaaccagag gtgataagca
     5941 cgcccagcgt cgccagcgac agtgccggtc ctaacgccac acgaaaggaa ctggcctgag
     6001 tgcgcatccc gccgtcgagc aaaataatcg ccagtgccag gttacttacc atgtaggcga
     6061 aggggtaatt atcaaacggg ataccgccga cgccatcgac tcctgccagc atgccgatcg
     6121 ccagaaaaat aaccagaata ggaatgccaa gacgggaaga aaatgaacta agtaatatac
     6181 tgctggtgac aaggatggat cctaagatga aaaggctaat tattgttgtg gcatccaacg
     6241 gtcggttact cctgattacg ctgtctctta tataaaccct accatattag cggcagagac
     6301 agcgttttac ttagccctga agcgtgattt ttttacattt taagaacagg atgaccgcta
     6361 atcgtcaact gagtgccttc ccgggtgtta ttcagaatgg catgtgcgcc caacggcagc
     6421 gtaaccgtgc gttgttcatg accaaaatcg aggccggtaa tgagtggaat cgacaggcgg
     6481 gaacgcaaaa acgcgtacac tgactccagg ttgtaacctg cgtcataatc attgggcgtg
     6541 ctgccgctaa agctaccgag aatgatcgcc ttctgacgtg gcaaaattcc cgcatgatag
     6601 agctgcaaca gcatacgttc gtcccggaaa gggtgctcgt taatatcttc cagcaccaga
     6661 ataccgttct caatttttgg catccacggt gtaccgatca gtgaaatcag catcgcaaga
     6721 ttgcctcccc acaacgtgcc ttcggcccga catgtcgggc cttccccttg ccattcaatg
     6781 gtgaaggttt cattgcgtaa cgccagccag aaatggtgct cggtaaaggc gttcagttca
     6841 tccgcgccaa agtttgctac cagcatgggg ccgctaaagg tgatgacatt gccatgcgcc
     6901 agaagaccgc actgaatggc ggtaaaatcg ctatgcccgc aaatgagcaa cgggtcatgt
     6961 tgttggcgag ccaccagtgc ctgccagtca atatctgcca gtaaacgact ggcaccgtaa
     7021 ccgccgcgta cagccagcac gatggtgttg ggggtggtca gtctggcaag ggaattgaga
     7081 tcttccaggc gctccgtttc cgtacccgca aaacgctcac aacgacgggc aataacctcg
     7141 acgttattga cctgatgccc ctcgtcggta aggcgctgga taccacgcag cgcggcgtgc
     7201 tgtttaatgc agtaacccga tggggcaatt aagtgaaaca gagacatggc aattccttgc
     7261 tgacaacaga aacgaaatgt atatcatgcc gcttaggtgt gccgttgtca cctcaacggc
     7321 gattccaggc tataaggata gaagaagtga aattgagatg gtttgccttt ttgattgtgt
     7381 tattagcggg ttgttcatca aagcatgact atacgaaccc gccgtggaac gcgaaagttc
     7441 cggtgcaacg tgcgatgcag tggatgccaa taagccagaa agccggtgca gcctggggcg
     7501 tcgatccaca attgatcacg gcgattatcg ctatcgaatc gggtggtaat ccgaatgcgg
     7561 tgagtaaatc gaatgccatt ggtttgatgc agttaaaagc ttcaacctcc ggacgtgatg
     7621 tttatcgccg tatgggctgg agtggtgagc cgacgaccag cgagctgaaa aatccggagc
     7681 gtaatatttc aatgggggcg gcttacctga atattctgga aaccggcccg ctggcaggca
     7741 ttgaagatcc gaaggtactg caatatgcgc tggtggtgtc atacgctaac ggggcaggtg
     7801 cgctgctacg gactttctcg tcagatcgga aaaaggcgat cagcaaaatc aacgatttgg
     7861 atgctgacga gttcctcgaa cacgtagcgc gaaatcaccc tgcgccgcag gctccgcgct
     7921 atatctacaa acttgagcag gcactggacg cgatgtaaat cagtcgcgca ctttgtccgc
     7981 tttttcccgg gcttctcgct cgagagagaa aataatccgc tgtaattgcc gctccaccgt
     8041 cgggctgacg ttaagaaaac ggaagctcag acggggagtg gtgatggttt cattcttgcc
     8101 atcaatcact ttgcgctcgc tgatagagat taactgggcg tcaaagtgaa aaacacccca
     8161 ttgccccatg ttgacttcaa tctgagcgaa gcgcatgcct tcttgtaatt cggcaggctt
     8221 tgctgtttcc agtaatgcgc ccatgccgcc taacgacaaa tcatacaggc ggaaacgtaa
     8281 cgtactgtta tccgccagtt tggtctggca aaaataaggc ggatggagtg gggcggagat
     8341 gcggaaatat cggcgtcgtt gtacaaacca taaggtggga ggcggtacgg tgataaatgc
     8401 cggaagctgc aagtattcac tctgctgtag ttgttcaaca gtaaactcga ctttcgcacc
     8461 ctgagtttcg gcggtaatgg taatgtgctg tgcctttagc acagcgatgt tgtcttcggc
     8521 ttgactgccg aaatccagca ccagtttatc cggggttatt gccagtattt tgctgatcag
     8581 ctgcccgcca ttccaactga gacgcaaagg aattgcggct ttgtgcaaat cgcgtaacac
     8641 gcccaggacg gctaacggat tttgtttcag gaactgctca tggtaatgac tcacgcggaa
     8701 aaactcctga ctgacaaact gtctttggtt tatcggtcac agttaacaaa acttaataca
     8761 aatgcgtgaa tattttttac atgttgttct taaatcagcc gacatacgcc cagcatcgat
     8821 cccgcccttg cctatactta gagcgttgac gtaagcattt cttgcgtccg attcatcgaa
     8881 acgagggcct gaacatggga attattgctt ggattatttt tggcctgata gccggcatta
     8941 tcgccaagct aatcatgccg gggcgtgatg gtggtggatt tttcctgacc tgtattctcg
     9001 ggatagtcgg tgcggtggtc ggcggctggc tggcgaccat gtttggcatt ggcggctcca
     9061 tcagcggttt taatttgcac agcttcctgg tggcggtg
//
LOCUS       DAZOYL010000153         9085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZOYL010000153 DAZOYL010000000
VERSION     DAZOYL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.153"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            123..659
                     /gene="hpt"
                     /locus_tag="ACHUOY_002631"
     CDS             123..659
                     /gene="hpt"
                     /locus_tag="ACHUOY_002631"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR0683378.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            complement(700..1362)
                     /gene="can"
                     /locus_tag="ACHUOY_002632"
     CDS             complement(700..1362)
                     /gene="can"
                     /locus_tag="ACHUOY_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR0683379.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            1471..2397
                     /locus_tag="ACHUOY_002633"
     CDS             1471..2397
                     /locus_tag="ACHUOY_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0683380.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            2394..3164
                     /locus_tag="ACHUOY_002634"
     CDS             2394..3164
                     /locus_tag="ACHUOY_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0683381.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            3269..3709
                     /locus_tag="ACHUOY_002635"
     CDS             3269..3709
                     /locus_tag="ACHUOY_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR0683382.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            3773..5002
                     /locus_tag="ACHUOY_002636"
     CDS             3773..5002
                     /locus_tag="ACHUOY_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0683383.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5006..5386)
                     /gene="panD"
                     /locus_tag="ACHUOY_002637"
     CDS             complement(5006..5386)
                     /gene="panD"
                     /locus_tag="ACHUOY_002637"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR0683384.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            5660..6556
                     /gene="rpnC"
                     /locus_tag="ACHUOY_002638"
     CDS             5660..6556
                     /gene="rpnC"
                     /locus_tag="ACHUOY_002638"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR0683385.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            complement(6848..7699)
                     /gene="panC"
                     /locus_tag="ACHUOY_002639"
     CDS             complement(6848..7699)
                     /gene="panC"
                     /locus_tag="ACHUOY_002639"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR0683386.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(7711..8505)
                     /gene="panB"
                     /locus_tag="ACHUOY_002640"
     CDS             complement(7711..8505)
                     /gene="panB"
                     /locus_tag="ACHUOY_002640"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR0683387.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(8618..>9085)
                     /locus_tag="ACHUOY_002641"
     CDS             complement(8618..>9085)
                     /locus_tag="ACHUOY_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0683388.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
BASE COUNT         2179 a         2141 c         2383 g         2382 t
ORIGIN      
        1 gctttattaa cagattccgc gaatgaatag ttttactggt atactgcgtg tcttgcgctt
       61 tgttgcggtg ccaaaacctg cccgtgcgaa gtgatttgtt tttaaatcat atggttagag
      121 atatgaaaca tactgtagaa gtaatgatcc ccgaagcgga gattaaagcg cgtatcgccg
      181 aactgggtcg tcagattact gagcgttaca aagacagcgg cagcgatatg gtgctggtgg
      241 gtctgctgcg tggctcattt atgtttatgg cggacctgtg ccgtgaagtt caggtatctc
      301 atgaagtcga ctttatgacc gcctccagct acggtagcgg catgtccacc acccgtgatg
      361 tgaaaatcct caaagatctg gatgaagata tccgtggcaa ggacgtgctg attgttgaag
      421 atatcatcga ctcggggaat acactgtcga aagtgcgtga gatcttaagc ctgcgcgaac
      481 cgaagtcgct ggcgatttgt acgctgctgg ataaaccgtc ccgtcgtgaa gtgaacgtcc
      541 cggtagaatt tatcggtttc tcgatcccgg atgagtttgt ggtgggttac ggcattgatt
      601 acgcacagcg ttaccgtcat ctgccgtata tcggcaaagt gattctgcgg gacgagtaag
      661 tgtgaagttg ccggatgtgt tgcatccggc atggcatttt tatttgtggt tggcgtgttt
      721 cagcttgagg ttggaaatcc cgtgacggta acgttgctca agggtttcgc ggttggtcgc
      781 tgtgacatcc agatcacgca gcaagccgtc gtgaatgccg taggcccagc cgtgaatggt
      841 aactttctgc ccgcgtttcc acgctgattg cataatggtg gagtggccta ggttataaac
      901 ctgttccatg acgttcagtt cacacaaggt atccagacgg cgctcttgcg gcatttcgcc
      961 gagcaatgag ctatgtttga accagatatc gcggatatgc agcagccagt tgttgataag
     1021 ccccagttcc gggttttcaa cggcggcttg tacgccgccg caaccgtagt ggccacagat
     1081 aataatgtgt tcaacttcga gtacatccac tgcatactga accacggaaa ggcagttcag
     1141 gtcggtgtga atgaccaggt tagcaacatt acggtgaaca aagagttcgc ccggctcaag
     1201 accggttaaa cgttctgcag gaacgcgact gtcggaacat ccaatccata gaaagcgcgg
     1261 tttttgcgct tgtgccagtt tctcaaaaaa cccgggatcc tcttccacca gcatttttga
     1321 ccatagtgca ttgttgctga tgagtgtatc tatgtctttc atggaggtta acgacctgta
     1381 accaaataat tacgtttggc taatataggg caactccggg acgatttaaa ccacagataa
     1441 agtgtaagaa cgtaaggtaa gtaaaaattt atgaccattg cactggaact tcaacagctt
     1501 aaaaaaacct atccaggcgg cgttcaggcg cttcgtggaa tagatttgca ggtcgaagcg
     1561 ggtgattttt atgcgcttct cgggccgaac ggggccggga aatcgaccac tatcggtatt
     1621 atcagctctc tggtaaataa aacctccggg cgggtcagcg tatttggtta cgatctcgag
     1681 aaagatgtcg tgaacgctaa acgtcagttg ggactggtgc cgcaggaatt taacttcaac
     1741 ccgtttgaaa ccgtgcagca aattgtggtg aatcaggcag ggtactacgg tgtggagcgc
     1801 aaagaggcgt acatccgcag cgaaaaatat cttaaacaac tcgatctatg gggaaaacgc
     1861 aacgaacgtg cgcgtatgtt atctggcggg atgaagcgcc gtttaatgat tgcccgtgcg
     1921 ttaatgcatg aacctaaact actgattctc gacgaaccga ccgcaggcgt ggatattgaa
     1981 cttcgccgct caatgtgggg ctttttgaag gatttaaacg acaaaggcac caccatcatt
     2041 ctcaccacac actacctgga agaagcagaa atgctgtgcc gcaatatcgg cattattcaa
     2101 cacggtgagc tggtggaaaa tacctcgatg aaggcgctgc tggcgaagct gaaatcggaa
     2161 acctttattc tcgatctcgc accgaaaagt ccgttaccga agctcgatgg ctatcagtat
     2221 cgactggtcg ataccgcgac gctggaagtt gaagtgctgc gtgagcaggg gatcaacagc
     2281 gtatttacgc agttaagtga gcagggcatt caggtattaa gtatgcgtaa caaagctaac
     2341 cgtctggaag agctgtttgt ttcactggtt aatgaaaaac aaggagatcg cgcatgatgc
     2401 atctttactg ggtggcgcta aaaagcatct gggcgaaaga gatccatcgc tttatgcgta
     2461 tctgggtgca gacgctggtg ccgccagtca tcaccatgac cctttacttt attatcttcg
     2521 gtaacctgat tggttcgcgt attggcgata tgcatggctt cagctatatg cagttcatcg
     2581 taccggggct gatcatgatg tcggtgatca ccaatgccta cgccaacgtt gcgtcatcat
     2641 tttttggtgc caagttccag cgtaatattg aagagctgct ggtagcgccg gttccgactc
     2701 acgtcattat tgccggatat gtcggcggtg gcgtggcgcg tggtctgttt gttggcattc
     2761 tggtgacggc aatttcactg ttttttgtgc cgtttcaggt gcattcgtgg gtattcgttg
     2821 ccttaaccct ggtgctcacg gcggtgttgt tctcccttgc gggtttgctg aacggtgtgt
     2881 ttgccaaaac gttcgatgac atcagcctgg tgccaacctt tgtgttaacg ccactcacgt
     2941 atttgggtgg ggtcttttac tcactgactt tgttgccgcc gttctggcaa gggctgtcgc
     3001 acctgaaccc aatcgtttat atgatcagtg gtttccgcta cggcttcctc ggtatcaatg
     3061 atgttccgct ggtcactacc tttggcgtac tggtggtctt tattgtggcg ttttatttga
     3121 tctgttggtc gctgatccaa cgtggacgtg gtttgcgtag ctaaggctat ttcctctcct
     3181 ctggatttgg gggagaggag ttttgacggc tatcaccctt tatcaacaat ggtcagggta
     3241 gactgatttt cggctaagga ggaaggcgat gttaggttgg gtaattacct gtcacgatga
     3301 ccgggcgcaa gagatactgg atgcgctgga gaaaaaacat ggggcgcttc ttcagtgccg
     3361 ggccgtgaat ttctggcgtg ggttaagctc taatatgctc agccgcatga tgtgcgatgc
     3421 tctgcatgaa gcggactctg gtgagggtgt catcttctta accgatatag ccggagcgcc
     3481 accgtaccgc gtggcttcat tattaagcca caaacactcc cgttgcgaag tgatttctgg
     3541 tgtcacgtta ccgttaattg aacagatgat ggcttgccgt gaaaccatga ccagttcaga
     3601 gtttcgcgag cgtattgtcg aactgggtgg gccggaggtg agtagtcttt ggcaccaaca
     3661 acaaaaaaat ccgcctttcg tcctcaaaca taatttgtat gagtattaac ccacgattct
     3721 tatagcgctt ttgctacaat aaaagcgttg tttcaccctc ggttattttt tcatgtacaa
     3781 acaagctgtt attctcctgc tgatgctgtt taccgcaagt gtcagtgccg cgttacctgc
     3841 ccgttatatg caaaccatcg aaaatgctgc ggtctgggcg caaattggcg acaagatggt
     3901 gaccgtgggg aatattcggg ccggacaaat cattgccgtg gagcccactg ccgcaagtta
     3961 ttacgcattt aattttggct ttggtaaagg gtttatcgat aaagggcatc tcgagccggt
     4021 tcaggggcga caaaaagttg aagacggttt gggtgacctc aacaagccgc tgagtaatca
     4081 gaacttagtt acctggaaag atacgccggt ctataacgcg ccgagtgtgg gcagtgcgcc
     4141 atttggggta ttggcggaca atttgcgcta cccgattttg cataaactga aagacaggtt
     4201 aaatcaaacc tggtatcagg tccgtattgg cgatcgactg gcctatatca gcgcgctgga
     4261 tgcccaaccc gataatggcc ttccggtgct aacttatcac catattctgc gcgacgaaga
     4321 aaacacccgt tttcgccata cttcgacgac cacctcggta cgcgctttca ataaccaaat
     4381 ggcctggctg cgcgacaggg gatacgcgac actgagcatg gcgcagctgg aaggttacgt
     4441 gaagaataag atcaatctcc ctgcgcgagc ggtggtgatt acctttgatg atggcctcaa
     4501 gtcggtgagc cgctatgcgt atcctgtatt gaaacaatat ggcatgaagg cgacggcgtt
     4561 tgttgttacc tcgcgcatca aacgtcaccc gcagaagtgg aacccaaaat cgctgcaatt
     4621 tatgagcgtt tctgagctta acgaaattcg cgatgttttt gattttcagt cacataccca
     4681 ttttttgcat cgggtggatg gctatcgccg acccatatta ctgagccgta gtgagcacaa
     4741 tattctgttt gattttgcac gttcacgccg cgctctggcg caatttaatc cgcatgtctg
     4801 gtatctttcg tatccgtttg gcggatttaa tgacaaagcc gtgaaggcag caaacgatgc
     4861 cggatttcac ctggcggtga caaccatgaa aggcaaagta aaaccggggg ataatccgtt
     4921 gttactaaaa cgactttata tcttaagaac ggattcgctg gagacgatgt cgcggctggt
     4981 gagtaaccag ccgcagggat aacaatcaag caacctgtac cggaatcgct ttcgcggtac
     5041 gtttcatttc attgtcgcct tcaaaatagg cgacgttggg tcgccaggtg cgagcttctt
     5101 catctggcat ggtaacgaag ctggcgatga tgacaatatc gccgacactg gcgcagtggg
     5161 ccgccgcacc gttaacagaa ataattctcg aaccgcgttc tgccgcgatg gcataagtgg
     5221 agaaacgctt gccgttggtg acattccaga tatcaatggc ttcgttttcg agaataccgg
     5281 ctgcgtcaag aaaatcctgg tcaatggcgc aagaaccttc atagtgcagg tccgcatgag
     5341 tcactttcac gcggtggagt ttgccctgca gcatcgtgcg aatcataact tctacctttc
     5401 taccctgtcg ttaacgaagc aggcgatgcc tgctttgagg aaattctcac gcagtattgc
     5461 ccgatttttg tttagtgtct actcatctga cggcatttac atcagcagtt tgcgtaccgt
     5521 gccgaagcgt gccatgtaac ggctgtaacc aattgaaatt tctctgaacg ctgctcggta
     5581 aacactaaag aggtggctga cgatggcacg ccttgactgg atactgcctt cacgcaagac
     5641 aacacggaag gcagggaaga tggatgcacc gagtaccaca ccgcatgatg cagtgtttaa
     5701 acaattttta atgcacgcgg agacggctcg cgactttctg gagatacatt tgccagtgga
     5761 attacgagaa ctttgtgacc tcaacacgct tcatttagag tcggggagtt tcattgaaga
     5821 gagcctgaaa ggacacagca cggacgtgct ctattccgtg caaatgcagg gcaatcccgg
     5881 ttatctgcat gttgtgattg aacaccaaag caagccggat aagaaaatgg tctttcgcat
     5941 gatgcgttat tctattgctg ccatgcaccg gcatctggag gcggatcacg ataagctgcc
     6001 gctggtggtg ccgatactgt tttatcaggg cgaggccaca ccttatccgc tctcaatgtg
     6061 ctggtttgat atattttact cgccggagct ggcacgacgc gtctataaca gtcctttccc
     6121 gctggtggat atcaccatca caccggatga cgaaatcatg caacatcggc ggattgcgat
     6181 tctcgaacta ctgcaaaaac atattcgcca gcgcgactta atgttattgc tggagcaact
     6241 ggtcacgctg attgacgaag ggtacactag cggaagtcag ttagttgcca tgcaaaacta
     6301 tatgctgcaa cgcggtcata ctgaacaagc ggatttgttt tatggtgtgc tgagagacag
     6361 ggaaacggga ggggagtcta tgatgacgct ggcgcagtgg tttgaagaga aagggattga
     6421 gaaggggatt cagcagggaa gacaggagga aaggcaagaa ttcgcccagc gttttctgag
     6481 taaagggatg tctcgggaag acgttgcaga gatgacaaat ttatctcttg ctgagattga
     6541 taggctgatt aactaagttg tctgcgttat gaataattgc ttacagacgc catcgccatg
     6601 acgtggggca aattaataat tggcgccatc agccgtagcg ccagttatac tcgtcatact
     6661 ttaaattgca tgtgctgcgt ctgcgttcgc taaccccagt cacttactta tgtaagctcc
     6721 tggggattaa tgagagacat ccatgtctct caccgaaggc cagcctgcgg ctgggcaaat
     6781 tcgttcctga cgaatttgtc actcgcttgc cgccttcctg caacttgaat tatttagagt
     6841 aaaagcatta cgccagttcg acgattttgt tgtcgatcag gcgggcatcg ccaagccagg
     6901 cggctaccag aattacagct cgtttgctgg tttcagagac ttccagcaac gtgtcggcat
     6961 cgcgaatctg aatatcatcg gcgcggaagc ctttttcatt cagttcttgc cctgcaatgg
     7021 caataatttc atcaagatcc cgctcgccag cctgcaattt gtcggcaatt gaacttaaaa
     7081 ctttgtacag accgggcgca attttgcgtt gttccgccgt cagataacca ttacgggaac
     7141 tgagcgcaag gccatctttg gcgcgcataa tcgggacgcc gacaatttca atatcgaagc
     7201 ccatatcggc aaccattttg cggattagcg ccagttgctg aaagtccttt tcaccgaagc
     7261 aggcgatgtc cggctggacc aggttaaaca gcttgctgac gatagtcgaa acgccgcgaa
     7321 aatgccccgg acggctcgcg ccttccagca tggtcgaaag cccgggaacg tcaacgtaag
     7381 tatgggtttc agtaccgttc gggtagatct cttttaccga aggggcgaaa actaaatcca
     7441 ctttacgttt gtttagcttc tcgcagtcct cctgcaaggt ccgtggataa cgtgccagat
     7501 cttccgggcg gtcgaactgc atcgggttaa cgaaaatact gacgacgacc acatcggcgc
     7561 gggctttggc ttcgtcgacc agcttcatat ggccatcgtg cagattaccc atagtaggca
     7621 ccagcgccac gcgcttgcct tccatacgca ggcggcgaat ttgctgacgc agcagcggca
     7681 gggtttcgat aattaacaca acgtgactcc ttaatggaaa ctgtgttctt cgcccggata
     7741 aacgccggac tccacttcag ccatatactg ccgcacagcc gcgcggatgt cgcccgtttc
     7801 ggcgaggaaa tttttagcga atttaggaat gtgaccgccg gtaatgccga aggcgtcgtg
     7861 catcacgagg atctgcccgt cagtgacgtt gcctgcgcca atgccaataa ccgggatcgc
     7921 cagtgcttcg gtaatacgtt ttgccagttc aatcggcacg cattccagca ccagcagctg
     7981 tgccccagcg gcttctaagg ctaatgcatc gctgagcagt tgatcgcccg cttcatcgcc
     8041 gcgcccctga actttgtagc caccgaaaat attcactgac tgtggggtta aacctaagtg
     8101 accacataca ggaacggcac gttcggtcag catttttacg gtttcgacca gccactcacc
     8161 gccctcaatt ttgaccatat tggcaccggc acgcataacc gttgcggcgt tttcaaaggc
     8221 ttgttccggc gtggcatacg ccataaacgg caggtcagcc aacagcaggc agtttggcgc
     8281 accacgacgt acggaggcag tgtggtaggc gatatcggca acggtcaccg gcagggtgga
     8341 gtcgtgaccc tgaaccgtca tgcccagcga gtcgcctacc agcatgacgt taagtccttc
     8401 atcagcaaat agtttcgcga agctgtagtc gtaagcggtg atggtcgcga aacgcttttt
     8461 atcctgctta catttctgaa gtgaagcaat ggtggtcggt ttcataacgt gtcctgataa
     8521 atgatgtcgt gttgcctggc attttatcag tcacattggt gggggcaatg atttatcaat
     8581 taccgcaccg ccagatgatg gcggtgcggt ggacttatta agggtaggtt acctggaaag
     8641 tactggtggc tttaaactca ccagcttcta ttggtatagt tccatcctgt tgtagcgtgg
     8701 cctggaaatg gagagggtat gttatacctg tatcttgatc cgggtaaact cctcctgttg
     8761 tatcgtcttt gcctctcggg tcgtaatctt tataaacaga atttgaatca ttaggtttca
     8821 atgtcattag aggattttta ctagttgcta aaccttctat gagtacaccg actccttttg
     8881 ctgcgtcatt acctgtgaga gtattaccaa ggagttgccc gttttcagta ccaacctttg
     8941 ttgaaactaa ttttgtctca atattagtca cacgaacgca gttttgaagt gaaatatcaa
     9001 aaggaaccgg tgtggcaccg tttctgattt gttctgcact atattcaccc atttttaccg
     9061 tagaaccagt cacagaagtt ccact
//
LOCUS       DAZOYL010000154         9082 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZOYL010000154 DAZOYL010000000
VERSION     DAZOYL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.154"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /locus_tag="ACHUOY_002642"
     CDS             complement(<1..101)
                     /locus_tag="ACHUOY_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683389.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHUOY_002643"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHUOY_002643"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0683390.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHUOY_002644"
     CDS             complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHUOY_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR0683391.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHUOY_002645"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHUOY_002645"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR0683392.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHUOY_002646"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHUOY_002646"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR0683393.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHUOY_002647"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHUOY_002647"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR0683394.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHUOY_002648"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHUOY_002648"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0683395.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
BASE COUNT         2011 a         2511 c         2136 g         2424 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cagcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagtt cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 cttgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aa
//
LOCUS       DAZOYL010000155         9074 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZOYL010000155 DAZOYL010000000
VERSION     DAZOYL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.155"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(506..1273)
                     /gene="fecE"
                     /locus_tag="ACHUOY_002649"
     CDS             complement(506..1273)
                     /gene="fecE"
                     /locus_tag="ACHUOY_002649"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR0683396.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="ACHUOY_002650"
     CDS             complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="ACHUOY_002650"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR0683397.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="ACHUOY_002651"
     CDS             complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="ACHUOY_002651"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR0683398.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="ACHUOY_002652"
     CDS             complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="ACHUOY_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR0683399.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="ACHUOY_002653"
     CDS             complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="ACHUOY_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR0683400.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="ACHUOY_002654"
     CDS             complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="ACHUOY_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR0683401.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="ACHUOY_002655"
     CDS             complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="ACHUOY_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR0683402.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8188..8256
                     /gene="ykiE"
                     /locus_tag="ACHUOY_002656"
     CDS             8188..8256
                     /gene="ykiE"
                     /locus_tag="ACHUOY_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR0683403.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8343..9040
                     /locus_tag="ACHUOY_002657"
                     /pseudo
     CDS             8343..9040
                     /locus_tag="ACHUOY_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1987 a         2509 c         2585 g         1993 t
ORIGIN      
        1 cactttttag aaaaatcgat ggtcagaaac tggattagca attccgttcc agggttgctt
       61 ttgatttacg ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat
      121 gaaccagctc cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tcccccccct
      181 gaaatctaaa cttagtcatg tcacgttttt gggtttctaa aattttaact tcgcgttttt
      241 cgttgccgta agggttatac agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg
      301 atcacgactg gtttgaaagc cgcgcccaag cctgtacagc tctggtttgc gttgattatg
      361 aacctgtcag cctaaagcaa gcggatggac gatgagtatt ggtaatcttt cagagtccgg
      421 aaaagttcag ccccagtctg aacaggcttg ctggcgccag tccagtttca ttcagtcgtg
      481 gtttggttct tacggcctgt gcaatctacc tcattaggca catcggcctg ccagataccg
      541 gctcggggtg tatttccgct tccacgctga atactgttct cagcaatcct ggggtcatca
      601 cctcttctgg tgtgccttgc gccataacat gtccgtttgc cattaccacc agttgatcgc
      661 agtaccggct agcctgatta aggtcgtgca gcacagcgac caccgttttc ccctgagtcc
      721 ggagttcgcc catcaaccgc atcaggtcca cctggtgatt gatatcaaga taggtggttg
      781 gctcatcaag taatacaacg ggcgtattct gggccaggac cattgccaga aatgcgcgct
      841 ggcgctgacc gccggaaagc tcggttaacc gacgaacggc aagatgattg atccgggtct
      901 ggttcatggc gacattaact cgtgcattgt cttcagcgga gagacgcccc cagagtgaca
      961 gccagggatt acgaccatac gaaaccagct cctggactgt gatcccctct ggcgttaaat
     1021 ggtgctgagg cagcagcgaa agcctgcggg ccaactggcg cgatgagagc atatttatgg
     1081 gattatcgcc gagaaatacg gtgccagact gcggcattaa aagccgcgaa aaacagttta
     1141 acagcgtcga tttcccgcaa ccgttaggac cgatcagggc ggtgatcttc cccgttggca
     1201 gtgagagtga aacgtcgtta agtaccttgt ctgttccgta attgaccgtc agattttcag
     1261 ttcgtaaagt catttatcgc attctcacaa gcaaccagac aaaccacggc gcaccgataa
     1321 tggcggtcag cacgccaacc gggagctcca gtgggggatg aataattctc gccagcagat
     1381 cggcaaccac caacagcaac gcacctgtca gggccgaaac aggcagcagt ctgcggtgac
     1441 gtccaccggt gatgctacgc atcatatgcg gcaccacgag accaataaag ctaatcgggc
     1501 cgcaggcggc cacgccggta gatgtcatgg cgacagctag taacaaagcc cagaatcggg
     1561 tatagggcac cgacacaccg agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga
     1621 gatcgcggca aaaactcagg ctcagcggca gaaataaaat catcagcggg atggcaatct
     1681 tcacaaagct ccagtcacgg ccccataagc tgccggtcag ccacagcagg gcgttgttca
     1741 catcctgcgg gcgcgagagc atcagataat ccgtcaggct ggcccagcat gcagaaagcg
     1801 ccacgccggt gagtgccagc ttcatcggct ggtgggtctt tgccagcatc ttcagcaata
     1861 tcaaccccgc catgccgccc gcaaaggcca gcagcggcag caccatcacg ggcagtgacg
     1921 gcataagaag tagagccccc acagaggcca ggctggcggc atggttaacg ccgagaatat
     1981 ccggtgatgc cagagggttg cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg
     2041 ctgcaccgac aaacagtgcc agcagcaagc gcggcagtcg gtactccatc aatacataat
     2101 aatgctcgcg tccggcctgc cagtcggtca gcagcgcgcg ccacggcacg gggatcactc
     2161 ccatatggag tgataacagc gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg
     2221 caattttcat cctcgcctcc tcacaagcca gacaaagcaa gggctgccaa tcagcgccag
     2281 cactgcgcct gcgggcagat ctccggggaa ggccagcgcg cgtgcgagta catctgccag
     2341 cagcatcagc gtggccccca gcagcatgct cactggcagt acgttgcgct gatcgaagcc
     2401 tgcccagaag cgcgccagat gtggcaccag cagaccgata aacgccaccg gacctgccac
     2461 gctgacgcac gcaccaacca gaagcagcac taacatattg atgaccaaac gtagcctcgt
     2521 caggttcact cccagcgtat gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt
     2581 cgccagcagc aacacgacag ggactgcagt gaccaccacc ggcaagagct gccagacatc
     2641 ctgccagcgg gcgtgggaca ctccgccagc cagccagtaa aagatgccgt aagcatgatc
     2701 ttcggccagc agcagggtga tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc
     2761 cgcgaggatc agtttgtttc tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac
     2821 cagcagccag ctcacgccgc ccccgcatgc cgcgatgaac gacagagaat agcctgcaat
     2881 cggcgtcgga ctcagcgcgc tggtaagcgc catagccagc gccgcgccgc tgttaatgcc
     2941 gagcagtgaa ggagaggcca ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag
     3001 cgccaggctt gcgccgatca gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac
     3061 cagcgcttct ggtagcgttg gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc
     3121 ggaaacagga atggccgagt agcaaaacag actcagccag aaaataataa taagtgctgc
     3181 aacgggaagc ccccacagca gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc
     3241 ggctgatggt ggaagatttt taccgtgtca gcggcaatac gctctgcagc aaaaataccg
     3301 cgcatccgcg cccaggtgtt actgtcgacc gaagcaacct gctgcttctg cgcggcggtt
     3361 aacatctgcc agagcggatc ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg
     3421 gcaaccagca gccaggcagg attgaccgcc agcagttgct ccaggccgat ggacggcatg
     3481 gacgcacccg ccatcgcagc gggaacgttc agccccagag aggccagcac gctgccggtc
     3541 caggtctcct gagtatgcag gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt
     3601 gtccctttgg gaagctggct ggcccactgc gccatcctct ctttatgttg ttccagacgt
     3661 gcctgcatct ctcgcttttt acccaccatt tcgccgatga tagccgcaga ttgcaaattt
     3721 tcagcgtagg tttcgttgcg ggacttaagc agcagtaccg acgcgatttg ctgcaaggcg
     3781 atgtaaaccc ccgcatggcg actgctgtcg gcaatgatca ggtctggttt cagagcggca
     3841 atggcttcca ggctcggctg cgcgcgcgtt ccgacggact gccacggttt caggtgcgca
     3901 cgcacttcgg gcaggatgcg ttttgcatcg ttatcgtcgg caataccgat cgggctgacg
     3961 tccacggcgg ccagcgcatc ggcgaacgag agttccagca ccacaatccg ttgtggcgtt
     4021 ttttcgagtg taaacgtgcc gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc
     4081 accagcagca ggcctgcaaa aagaaaacgg ataaatgcca acataatcac attccagcta
     4141 aaagcccggc aagccgggcg ttaacacatc agaacttcaa cgccccctgc atatacagcg
     4201 tgcgcggctg gcctgcatag atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt
     4261 cctggtcgaa gatgtttttc acaccgaatg ccaggttcag atctgccatc tgcgggccaa
     4321 agtcatacac cacgcgtgcg ccccagagca tgaagccggg aatgcggccg gtactgccgt
     4381 cggcgctctc tttcaccgta ttggcgttat ccgcaaactg gctggactgg aaatcgctgt
     4441 tcagattgaa catccagttt cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg
     4501 gggagaatgg taccagattg ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat
     4561 acgcatagct ggcgtagatg gaaacgttat caagcgttgg cgttagcgta cccagatcgt
     4621 aacgtgcctg cgtttccagc ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt
     4681 tggtctggtt ggagtcgtac tgattgttaa agttaatcag gaacagcccc atttccgccg
     4741 tcagcgcgcc gtcgtcgtag cgggtaccga gttcccaggt tcgcgctttt tccggttcca
     4801 cattgccgct ttgcacagcc ttgccaatct ggctgtactg tacggtgccg aacgagcctt
     4861 cagtgtttgc ataaagattc cagctgtcag tcaggtgata gagcacgttc aacgccggaa
     4921 gcggtgcgtt atagctcact tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact
     4981 cgatatgttc gaaacgcata cccggcgtga tggtccagtt gccgatgtcg attttgtcat
     5041 ccagatacca ggcgtgcgcc tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc
     5101 cggacggcaa ctgcccgctg ctggtggcgg tgtagtaacg catttcatgc gttgattcat
     5161 tcacatagcg atagcccacg cccacttcgt gcgcggaagg gccgatcata aagatctggc
     5221 tgtagcgtgg ctcaataccg cgcacccagt agttacgcgg cgagagggtg atgcgtttgc
     5281 cttgctccag gtagccgctg cgcagggttt gggtgtagaa cccctgaatg ttgaatttat
     5341 gctggctgtc tggctggaac tggtagccca ggctcgccag cttgcgacga ccccagaagc
     5401 ggtcatacgg gcgggtggat tgccagcgat cggcgtcgta atccgcgcga gacaggccac
     5461 cgggcatgtc ggcttcaccg tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat
     5521 ccggcgcata tttgcttttc agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc
     5581 agtcactgcc gcgcgtgccg gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc
     5641 cgcccaccat caggttgtgc gtctctttcg ggttgttttg tgaagaggtt gggctgagct
     5701 gaccttccac gcccgcctcg ataccaaagt cctgcggaat ggcacgggta acaaagttca
     5761 ccacgccgcc cacgctctgc ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa
     5821 tggcattcat gttgccgagc gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg
     5881 cgaaggggac ggggatgccg tccatcagga cggtcgagcg gctggcgagg cgcgggttca
     5941 ggccccggat gccaaagttc atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg
     6001 cgctgacgcc agggatgcgg ttaagtacct cacgcatggt ggttgcgccg gttttggcga
     6061 aatcctcacg gcggatcacg tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg
     6121 catcacccag ccagtcgccg accacggtca gggcatcttc ttttggtgcg ggcgcgggct
     6181 ccagcgtcca gctgttattt cccagcggtt ttacctgcag tccgctgccg tccagcagtt
     6241 gttgcaggcc gctctcgacg tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg
     6301 tcaggctggc gtcaaccgag agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt
     6361 tatcgagcga tcccggtgcg atattaacct gtgcagcaaa agcggaaaac gagagaccgg
     6421 ccagcggcag caggctcagg cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc
     6481 gtaacggcgt cataccttcc ccatcatcat ttttgttgtg ttcagctatg agtcgaacga
     6541 gaagggtaaa aaggacaatc gaaataagaa ttattttcct tacagtggtg aaatgtttat
     6601 ccagtaccgc gtaatagact gaattttaac gggaagcgtt tgcgcgataa cgttcaggat
     6661 cgcatcggta tttttcagcg ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc
     6721 gcagcgcagc acgccgttgc ggtaacgggt tagcgtggct atcacctcac ccagcggttt
     6781 atcgctgaag ctcaggatgt ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac
     6841 tgcgccaaac tcagaggcgc tgaactgcag gctttcacca gcgttcacga tgcgtttttg
     6901 cgcgggggca ctggcgagaa gcacttccac ggcgtgctgc tgcacgtcaa gctgcgtgaa
     6961 attatcctgc tggcggacgg taaattctgt ccctaaagca gtgagctggc cctgacgggt
     7021 caggacacgg aaggggcgtt gcagggcatc tttcgcggta gtaatggcga tttcaccgta
     7081 ccagagccgg acggtgcgct gatgcgcatc aaaacgcata tccgccgcgc tttgggtatt
     7141 cagcgtgagc agggagccat cttccagttg ctgacggctg actgtgcctt tggcggtgcg
     7201 gtaatctgcc cgcagacctt cgccggtttc cgactgccag agctgccagc ctccgccagc
     7261 gccgagcaac agcagtaatc ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa
     7321 cgcccggctg gcaacgtcgc caggcacacc gccaagctgg ttgcgcaggt tttcaacctg
     7381 ctgccaggcc cactggttat cctgatcctg ttcataccac tgttgccagc gcgcttcctg
     7441 ttgtgggctg acgcgctcgc cgcttagcac ggcataccag tgggaagctg aacgcagcgc
     7501 ctgacggcgg gaatcggtta acaaaggatt cataacccat actccagacg gaacagcagg
     7561 cagtgctcga cggctttcgc cacgtatttt ttcacggagc tgatggaaac accgagtttg
     7621 tgcgcaatct cgctgtatgt cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt
     7681 ttgccgttta gcccgtccag catgctgtcg aggagttgta gggtctcgag ttggctttcg
     7741 cgttcctcag gtgaaggcgc tcccccctcc ggcataagcg ccagcatctc cagatacgct
     7801 ttttccagcg cgtttcggcg aaacaggtcc accatcacgc gtttggcgat agtgcagagg
     7861 aaggagcgag gatcgcggat cgtcgagagc gtttcgctga ccattacccg caaaaaagtg
     7921 tcctgggcaa tgtcatctgc atcaaaagca gactggagtt tgcgcgtcag ccagcttttc
     7981 aaccagccgt gatgtgtgcc ataaagcgac tcgaatgtta aggaagctgt ggtagtggcg
     8041 cggtcagaca tgcggagtgc atcaaaagtt aattatcacg tagccatatt aatatgagaa
     8101 tagttatcat tacaattgga aataaaattg tttccaatag acatttttaa catgttgttt
     8161 ttctaagtgt tataaggtag gtataaaatg ggatggagcc tctgcttctg gcatgtgtcg
     8221 gtcagaatga ctcatgatgt ggtctgctat tattgacatc ctcactgccc taaaggatag
     8281 ggatttcggt aatgctgtca acttactgat ttagtgtatg atggtgattt taaggtgctt
     8341 gcgtggcttc catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta
     8401 acggcaaaag cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc
     8461 aactacagtt cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg
     8521 ccatgaatgg cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg
     8581 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag
     8641 tgatgtgatt gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg
     8701 tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt
     8761 cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt
     8821 cgtggtatag atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca
     8881 cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca
     8941 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
     9001 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttacctcctc
     9061 ccgaactcaa aact
//
LOCUS       DAZOYL010000156         9064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZOYL010000156 DAZOYL010000000
VERSION     DAZOYL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.156"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            71..802
                     /gene="artJ"
                     /locus_tag="ACHUOY_002658"
     CDS             71..802
                     /gene="artJ"
                     /locus_tag="ACHUOY_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR0683404.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(977..2104)
                     /gene="rlmC"
                     /locus_tag="ACHUOY_002659"
     CDS             complement(977..2104)
                     /gene="rlmC"
                     /locus_tag="ACHUOY_002659"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR0683405.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(2145..2633)
                     /locus_tag="ACHUOY_002660"
     CDS             complement(2145..2633)
                     /locus_tag="ACHUOY_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR0683406.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2693..3538)
                     /gene="potI"
                     /locus_tag="ACHUOY_002661"
     CDS             complement(2693..3538)
                     /gene="potI"
                     /locus_tag="ACHUOY_002661"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR0683407.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(3535..4488)
                     /gene="potH"
                     /locus_tag="ACHUOY_002662"
     CDS             complement(3535..4488)
                     /gene="potH"
                     /locus_tag="ACHUOY_002662"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR0683408.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4498..5631)
                     /gene="potG"
                     /locus_tag="ACHUOY_002663"
     CDS             complement(4498..5631)
                     /gene="potG"
                     /locus_tag="ACHUOY_002663"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR0683409.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5726..6838)
                     /gene="potF"
                     /locus_tag="ACHUOY_002664"
     CDS             complement(5726..6838)
                     /gene="potF"
                     /locus_tag="ACHUOY_002664"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR0683410.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7189..7665)
                     /locus_tag="ACHUOY_002665"
     CDS             complement(7189..7665)
                     /locus_tag="ACHUOY_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR0683411.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7753..8655)
                     /gene="rimK"
                     /locus_tag="ACHUOY_002666"
     CDS             complement(7753..8655)
                     /gene="rimK"
                     /locus_tag="ACHUOY_002666"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR0683412.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8716..>9064)
                     /gene="nfsA"
                     /locus_tag="ACHUOY_002667"
     CDS             complement(8716..>9064)
                     /gene="nfsA"
                     /locus_tag="ACHUOY_002667"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0683413.1"
                     /translation="GVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPR
                     LPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPF
                     ILDYLHKQGWATR"
BASE COUNT         2346 a         2491 c         2199 g         2028 t
ORIGIN      
        1 ttgatggcat tgttatccca tgccgcagac acggccaaaa atcataagat tgacagacgg
       61 gagttccatc atgaaaaagt tagttcttgc cgctttactt gcttccttta ctttcggtgc
      121 ttctgccgca gagaaaatca attttggcgt ttcagccacc tatccaccct ttgaatctat
      181 aggtgctaat aatgagattg tcggctttga tatcgatctg gcaaaagcct tgtgcaaaca
      241 aatgcaggca gaatgtactt ttactaatca cgcgttcgac agcctgatcc cgtcactgaa
      301 attcagaaaa tatgactccg taatctccgg tatggatatc acgcccgagc gtagcaagca
      361 ggtatcgttt accacgccct actatgaaaa ctcagccgtc gtgattgcca aaaaagatac
      421 ctacaaaacg tttgccgatc tgaaaggcaa acgtattggg atggaaaacg gcaccacgca
      481 ccagaaatat attcaggatc agcaccccga agtgaaaact gtctcctatg acagttatca
      541 gaatgccttt atcgatctga aaaatggtcg tattgatggg gtatttggtg acacagcggt
      601 ggtaaacgaa tggctgaaaa ccaatccgca actgggcgtt gctactgaaa aagtgaccga
      661 tccgcaatac ttcggcaccg gtctgggcat cgctgtacgt ccggataaca aagctctgct
      721 ggaaaaactg aataacgcgc tggcagcaat taaagctgac ggtacatatc agaaaatcaa
      781 tgaccagtgg ttcccgcagt aattgagact gatgacaaaa gtaagattgc ctgatgcgct
      841 gcgcttatct ggcccacatc aactctgcaa tgtattgatt tagcatgatt ttgtaggccg
      901 gataaggcgt acacgccgca tccggcatga acaaagcgca cctgatcagc aatctgaagc
      961 cgcacctgcg gcttttttat tgcttcacca gcagcgtcat cacttcatag tgcgcggtgt
     1021 gcgggaacat atcaaaaagc tgtacccgtt cgatgcggta acctggcagt tcgcggatat
     1081 ctttcgccat ggtttgggcg ttacagctgg agtagataat aaaacgcggt gccatcgttg
     1141 agagataatc acacagcggt ttaccaatgc cgcggcgcgg cgggttaacc agcaccagct
     1201 ccggcacatc cccctgagcg gtggcaaact gagtggagtc cagcgcctga aattgcaaac
     1261 gcgttaagcc cagttcagcg gctgactgct ttgcacaggc aatggcctct gatgcaattt
     1321 cgatcccggt taactgcatg tcaggcgtcg cgcagtgtaa accaaagcca cccacaccgc
     1381 agaacagatc ccacatatgt ttaactggca gctgccgtac ccagtcgcgc gcggtagcgt
     1441 acagctggct ggcgaccgcc ggattagtct ggaagaaact ttgcggacgg atccacagcg
     1501 gtacgtcatt aaaacgctct gccagcgcct gttgttcagt caggtagatc tccgtctccc
     1561 cttccataat cgccatatgt accggctgaa tattgacggt aataactttc agttgcggta
     1621 gttgttcctg taaccacggc agcgccttac gcagttgtgc cagtttggtt tcagaacgca
     1681 gtacaaagcg cagcatcatg ccgccatcgc tctggctttc agtcagcaga atgtatttca
     1741 gttcgccacg cttacgcgcc acgttgtagg gcgttaaccc cgcacgggcg ataaacggtt
     1801 ttagcgccgc aaaaacgggc gcaaaagagg caggataaag cgggcagtca caaaggtctt
     1861 ccggcgtgcc atcgcgatgc agcataccga gcagtggttt ttcaacgcta ccactcacca
     1921 ccattttggc tttattacga aacccttgtt ccgggcctga caccggcgcg caccattcct
     1981 caaccggaaa atcggcgagc agatttttaa gatcggcggt tttagcggag agttgctctg
     2041 gaatcggctg cgttatccac tgacaggaac gacagcgacc cgcgtcgtaa agtgcgcact
     2101 gcatacattg accttcacat catcaggggc gacgattata cacattattg caactggaag
     2161 aaccgccgac tggtcgaggg aacgaacagc agcatcagga tgagcatatc cggcagcttc
     2221 tgcagcatca ggctatggaa gatttcacgt tttgattcac cgggaatgct gaacagctcc
     2281 ggataaccgt accccagcga agccgcccac aagtaacttg cggcggtgat ttgtgtcagc
     2341 agatatagcc agcgcgccca gcgacgccct ttcaccagtg aaaacgcaca ccagatctca
     2401 atgaaaacca gcaccagact gcttaaaaag accagcgtta aactccaggt ttgtacgctg
     2461 cgatgaatga actcgccaat accgcgcacg cccagcgtat tgaaaatcat cagcacgtcg
     2521 aggccacgga tcataataat ggcgagcgcc gccacctgca ccagcgcagg cacattcagg
     2581 cgagcatgag atgaagatgt tttcttaaaa aatcccaacg tttcgtcttc catgaaaaca
     2641 atgccgcggc atgcgcggca ttatgtagcc aggttggcaa attttagtgt cttcagccat
     2701 gtcttgcacg ctggatatcg cgtatccgct gtttttctgc gcgagccatc agataccagg
     2761 cgataaatcc gacaattccg accgcaccga gaattaacgt tgcaagggcg ttgatttccg
     2821 gattcactcc catccgcacg ctggaaaaga ccagcatcgg taacgtggtg gctcccggcc
     2881 cggaaacaaa gctggcgatc accagatcat caagcgacaa agtaaaagcc agtaaccagc
     2941 cagaaatgat cgcgggcatg atcatcggta gcgtaatgac gaaaaacact ttcagcggcg
     3001 tcgcaccgag atccatcgct gcttcttcta tcgagcgatc cagttcccgc aatcttgaag
     3061 aaataacgac cgccacataa gccgtacaaa aagtgacatg cgccagccag atggtgagca
     3121 taccgcggtc cgcaggccag ccaatggcat gagcaagcgc gacgaataat aacaacagcg
     3181 acaagcccgt gatgccatct ggcatcacca gcggcgcggt gatcataaag gcaaaaccat
     3241 ttgatccgcg aaacctgcca aaacgcacca gcaccaccgc cgcaatagtc ccgaggatcg
     3301 ccgccgccgt tgccgcacag gcagcaattg tcaggcttaa accaaccgca ctcatcatcg
     3361 cgtcatcgcg caataactca ccataccagc gcgttgacca gccggcccac accgtcacca
     3421 gcttcgagct gttaaacgaa tagataacca gcatcagcat tggcgcgtag agaaaggtaa
     3481 agcccagcag caaaatcaca atccgccagg gcgaacgaac taccggtaaa ttattcatcc
     3541 gtgttctccc acgctttttt gctggtgttt gtggaaccac attatcggca caattagcag
     3601 cagcaacatg atgatcgcta ccgccgaggc caccggccag tcgcggttat tgaaaaactc
     3661 ttgccataac acgcgcccga tcatgatgct gtccgggcca ccgagcagtt ccgggatcac
     3721 aaactcgccc acagccggga taaacaccag catcgatccg gcaataatcc cacctttagt
     3781 cagcggcacg atcacagtaa agaacgtttt cagcggtcgt gcaccgagat ccagcgctgc
     3841 ttccaccagc gaataatcaa tacgaatcaa cgcggtataa atcggcagta ccataaacgg
     3901 cacgtaagcg taaacaatgc cgatataaac ggccagattg gtatgcagaa tggtcagcgg
     3961 ttgatcgata acccccagcc acagcagaaa attattcagc acaccgttgt tttttaatat
     4021 tcccatccag gcataaacgc ggatcagaaa cgaggtccac gacggcagga tcaccagtag
     4081 taataaaata ttacgggtcg aaggcttact gtgcgccacc gcccacgcca gcggatagcc
     4141 gatcagtaaa cagcaaaatg tcgaaatcgc cgccacctgt aacgactgga gataagcatc
     4201 aaaatagagc ggatcgtcgg tcagttgcag aaaattaccg agattaagag tgatggaaag
     4261 ttgcccgtca gcccactcca tcagttcggt ataaggtgga atagcgcgcg ccatctctcc
     4321 caggcttatt ttaaagacaa tcagaaatgg cagcagaaac agcaagatca accagatata
     4381 tagcaacgca atgaccagtt tgcgcccatg cttcatttgc agctgcgaca accacagctt
     4441 aaatccgccc ggcggtttcg actgggcagc aggttcaagt gtactcatct ttgctcctta
     4501 aaccgtcagc accacacagc tgtccacttc ccagcacaaa cgcacttcgt cgccccaggt
     4561 cggtaaccct ttacgatgac gatgggcgtt ttgtagctgg gcgctgatca tctgcccact
     4621 tttcagtcga acgtgataca ccgaaagatc gccgagatag gcaatgtgta tcacctcccc
     4681 caccgcgaag ttacaaccat tggcgggcgg ctcttcgcaa agcatgattt tttccgggcg
     4741 cagcgctacg tgtaccggca cgttatcgac cactgaagca tccgcatcga ctttcagtgg
     4801 atgcaccagc cccggcgaat caagcaccag gccatcttcc tgacgctctt tgagtacacc
     4861 ttcaaagaca ttaaccgaac cgataaattc agcgctgtag cgggtagttg gatgctcgta
     4921 gatctcttcc ggctcgccaa tctggacaaa tttcccgcga ttcataatgg cgatacgccc
     4981 cgccatggtc atcgcctctt cctgatcgtg ggtgaccatc acacaagtca caccgacgcg
     5041 ctccagaata tccaccactt caagctgcat ccggtcacgc agctttttat ccagcgcgcc
     5101 catcggctca tcgagcagta atagtttcgg gcgtttggca aggcttcggg ccagggccac
     5161 acgctgtcgc tgaccaccgg aaagctgatg cggtttgcgt ttggcgaact cctgcatgtg
     5221 caccaggccg agcatctcat ggacccgact ggcaatttcc gctttcggta gtttgtcctg
     5281 tttcaggcca aaagcgatgt tctgttccac ggtcatatgc ggaaacagcg cgtaagactg
     5341 aaacatcata ttgatggggc gcaggtaagg cggcacctgt gacaaatcga cgccatcgag
     5401 cattatctgc ccggcagaag gttgttcaaa acctgccagc atacgcagca gcgtggactt
     5461 gccacagccg gatgcgccca gcagcgcgaa gatttcacct ttgtagatgg tcaggctgac
     5521 atcatcgacc gcatgctgac catcgtagga tttggtgagg ttgcggattt ctaatagcgg
     5581 cgtcagcgcc ttacgggttt tcgcctgcgg gcgagggata gcgtcattca cggctgctct
     5641 ccggcattga atgtgtgcaa gcgcaccatg atggtgcgaa ttgccggctg gtgtggtgcg
     5701 cccgcccggc atctaaggct gcggattatt ttccgctctt cactttggtc cacgcgcggg
     5761 tgcgcacacg gtcgattttc ggatcctgta ctttcagagt gaatagcttc gcacgaacgt
     5821 ccgccggagg ataaataccc gggttctcac ggacttccgc actcaccagc ggcgtggctg
     5881 ctttattggc gttggcatag aacacatggt cggaaatatg cgctactaca tccgggcgca
     5941 gcaggtaatt caggaactga taggcttcgt ctttgttttt ggcatccgca ggcatggcga
     6001 atacatcaaa gaacgccatc gccccttctt ttggaatcga gaacgagaca ttcactccat
     6061 tcttcgcttc cttcgcgcgg tttgacgcct gccagacatc acctgcccag ccgatagcga
     6121 cgcaaatatc gccgtttgcc aggtcgttaa tgtattgaga tgaatggaaa taacgaatgt
     6181 tcgggcgcag ctttaacagc agatcggttg ccggtccggt gtaatcatcc gctttagtgc
     6241 tgttgggatc tttgccgaga taattcaaca cggtagcaaa aacttcttct ggcgcatcca
     6301 ggaaagagac accgcagctt ttcagttttt ccagattttc aggtttgagg atcaagtccc
     6361 agctatcgac gggcgcgttt tcgcccagca cagctttaac tttatcaacg ttatagccaa
     6421 tcccggtcgt cgcccacata tagggcatag caaatttatt gtcgggatcg tgtttggcga
     6481 ccagcttcag cagctccggg tcgagattct tccactccgg caatttgctt ttgtccagcg
     6541 gctggaaaac tcccgcagtc aactggcgtt caagaaagct ggcagatgga accaccaagt
     6601 caaagccggt gctacccgcc attaatttgc cttccagtac ttcgttagag tcgaaaacat
     6661 cgtagacgac tttaataccg gtttcttttt caaaattggc caccgtgtcc ggggcgatat
     6721 aatcagacca gttataaatg tggagtgttt tttgttcagc cgcgagcgtg ccgacagaga
     6781 cggccatcag agcacccgca gccagacccg aaagccattt tttatttaag gcggtcatat
     6841 ctctttcctt ctgaaagttc gttaacaaac gaacaaaagt tgtcacacta aaagatatgc
     6901 aataaacgtg catatttttt atcggcgggg tgtaagaaaa gagaactctc tcccggccag
     6961 cactgctcgc tatataaaag catcgcaaga ataactttag ccagggtttg agactatagc
     7021 tcggattaaa aaacggtatt taacaaattt ttatgcgtat ttgctctatg tgataaagcc
     7081 aaatcgacac aagagaagtg aaataatctt taagaaaagg ttaaaaacag cagataattt
     7141 tgcagcgcgc agccactgca ccagcgactg cgtgctcgaa aggagttatc agtggaggaa
     7201 atagttttcg gtaacgttgt tttgcccttc atcgtcagtc ggtaacagca gttgatgggc
     7261 attagcttca agaatgacca tcgaaatctg ctcttcgctc tgacgcacga accatgcaaa
     7321 ctgctcatag gttacgccct gcataacgga taaagactgg caaaccacca gctttggcag
     7381 attatcatcc tgcatatcaa gaaatgcttt cacggtcagc gaactggcat tgatggcaga
     7441 taaatccgcc gccagcggca atacggctga aggtcggact tccgccatgg cagaaaacag
     7501 gatcgtgtta tcgatcagat cgattttggc atcaaagaca ccgtcgaaat tctgcatatg
     7561 gggcagatgc agagcctgac agttatcaca ttcaaaaaaa ctcatcccca ggtcatcgag
     7621 ccattgacgc agcgtatcca gaccaggaac gaccagcgat gtcataattt tgtacaacct
     7681 ctttcgataa aaagaccggc acagcttacg caaaaagcgc aggcaaaacc atgatcagta
     7741 atgtgattgc gactaaccac ccgttttcag gcaatattct gtcgtagcgt ggcgttcgat
     7801 ccagcggatc attttacccg cgatgtcgat accagtggtt ttttctattc cttccagccc
     7861 cggcgacgca ttcacttcca tcaccaacgg cccgcgatta gcacgcagaa tatccacacc
     7921 agcaacgtcc agcgccatcg ttcgcgcggc ttttatcgcg atttcacgct cctgtggtgt
     7981 gatactggcg acgcttgccg cgccgccacg atgcaaattg gaacgaaaat cgccctcttt
     8041 cgcccgccgt tcaatcgcag cgaccacttc atcgccaaca accagacagc ggatatcgca
     8101 cccttgcgcc tctttgatat attcctgcac cagaatatgc gcgttcagac cgcggaaagc
     8161 gtcaatcacg ctttccgccg cctgacgcgt ctccgccagc acgacgccaa ttccctgcgt
     8221 gccttcaacc aacttgacca ccagcggcgc accaccgacc atgtcgatta aatcgctggt
     8281 atcatccggc gaatgcgcaa tgcccgtgac aggcaggtcg atgccctgac gcgccagcag
     8341 ttgcatggaa cgcaatttgt cacgcgcccg ggcaatggcg accgactcat tgagcggata
     8401 gctccccagc atctcgaact ggcgcagtgc cgccgtccca taaaaggtaa tggcggtgcc
     8461 aatacgcggg atcactgcgt caaaatgggg taacttgcgg cctttgtagt gaatagaaga
     8521 cgccgcagga tttatgttca tgtagcaaga aagcggatca agaatttcaa ccaggtgacc
     8581 gcgctgtata gcggcttcac gcagccgctt acacgaatag agcgttccat cccgggacaa
     8641 tatggcaatt ttcaccctgc acctctctgt cagacctggt caaaagcccg cgtatcatac
     8701 atcgacgtgg cggttttagc gcgtcgccca accctgtttg tgcaaataat ccagaataaa
     8761 tgggcggctt tctttaatga ttgttcggcg gatatgatcg ctccaggtat cccggcgatt
     8821 attgctgcca cgggtgaggt aatattccgc cagttgctcg tcatactgcg ccagtgcgtc
     8881 tttatccagc ggttgatagc tgttttcatg caccaaaatg gaggccggta aacgcggctt
     8941 gagatccgga ttatccgcag gccagccaag gcacagccca aacagcggca gaacatgctg
     9001 cggtaatttc agcagtttcg tcaccgcttc aatattattg cgcaggccgc cgatatatac
     9061 cccg
//
LOCUS       DAZOYL010000157         9018 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZOYL010000157 DAZOYL010000000
VERSION     DAZOYL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.157"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            159..440
                     /gene="drpB"
                     /locus_tag="ACHUOY_002668"
     CDS             159..440
                     /gene="drpB"
                     /locus_tag="ACHUOY_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR0683414.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            480..1136
                     /locus_tag="ACHUOY_002669"
     CDS             480..1136
                     /locus_tag="ACHUOY_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR0683415.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            1203..2621
                     /gene="dcm"
                     /locus_tag="ACHUOY_002670"
     CDS             1203..2621
                     /gene="dcm"
                     /locus_tag="ACHUOY_002670"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR0683416.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2602..3072
                     /gene="vsr"
                     /locus_tag="ACHUOY_002671"
     CDS             2602..3072
                     /gene="vsr"
                     /locus_tag="ACHUOY_002671"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR0683417.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3061..3982)
                     /gene="yedA"
                     /locus_tag="ACHUOY_002672"
                     /pseudo
     CDS             complement(3061..3982)
                     /gene="yedA"
                     /locus_tag="ACHUOY_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            4155..5072
                     /locus_tag="ACHUOY_002673"
     CDS             4155..5072
                     /locus_tag="ACHUOY_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR0683418.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5151..5333
                     /locus_tag="ACHUOY_002674"
     CDS             5151..5333
                     /locus_tag="ACHUOY_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR0683419.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            5504..7198
                     /gene="dgcQ"
                     /locus_tag="ACHUOY_002675"
     CDS             5504..7198
                     /gene="dgcQ"
                     /locus_tag="ACHUOY_002675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR0683420.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(7195..8010)
                     /locus_tag="ACHUOY_002676"
     CDS             complement(7195..8010)
                     /locus_tag="ACHUOY_002676"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR0683421.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8308..8535)
                     /gene="yodD"
                     /locus_tag="ACHUOY_002677"
     CDS             complement(8308..8535)
                     /gene="yodD"
                     /locus_tag="ACHUOY_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR0683422.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            8698..8886
                     /gene="dsrB"
                     /locus_tag="ACHUOY_002678"
     CDS             8698..8886
                     /gene="dsrB"
                     /locus_tag="ACHUOY_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR0683423.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(8930..>9018)
                     /gene="rcsA"
                     /locus_tag="ACHUOY_002679"
     CDS             complement(8930..>9018)
                     /gene="rcsA"
                     /locus_tag="ACHUOY_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="with RcsB is a positive regulator of capsular
                     polysaccharide synthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR0683424.1"
                     /translation="IKTHNKQVIYHVVRLTDNVTNGIFVNMR"
BASE COUNT         2201 a         2182 c         2454 g         2181 t
ORIGIN      
        1 cagtaattat tgccgttgcg caaaatttat ctatttgttc aaaaaatgat tgagtcttga
       61 ctggctcatc caatgtggaa aaatgtgact tttatcacat aatagtacta agtctgaatt
      121 ttccgggtta tctcaaaatg gaatacggtt cgacaaagat ggaagagaga ctctctcgca
      181 gccctggcgg caaattggct ttgtgggctt tctatacatg gtgtggctat ttcgtctggg
      241 caatggcgcg ttacatctgg gtgatgagcc ggatccctga tgccccggtg tcgggatttg
      301 aaagcgattt aggctctacc gcgggaaagt ggttaggggc actggtcgga tttttattta
      361 tggctctggt cggggcattg ttaggcagca ttgcctggta cacccgacct cgtcccgcac
      421 gtagcagacg ctatgaataa aaactgctaa ggtggcgact ctttggcaac ggagcgcgta
      481 tggacttaca acactggcag gcacagttcg aaaactggtt gaaaaaccat catcagcacc
      541 aggacgcggc acagaagctg gcggcagatg acgatgtcga tatgctggtg attttaaccg
      601 cctgttattt tcacgatatt gtgagcctgg cgaaaaatca tccgcaaagg cagcgttctt
      661 caatcctggc ggcagaagag acgcgtcgcc tgctccgtga agagttcgag caatttccgg
      721 cggagaaaat cgaggccgtt tgtcatgcca ttgccgctca cagtttcagc gcgcaaatcg
      781 cccccttaac aacggaagct aaaatcgtcc aggatgcgga tcggctggaa gccctggggg
      841 cgattggcct ggcgcgcgtg tttgccgttt caggggcatt gggcgtggcg ctgtttgatg
      901 gtgaagatcc gttcgcacag catcgcccgc ttgatgataa acgctacgcg ctggatcatt
      961 tccagactaa gttgctgaag ctaccgcaaa ccatgcaaac cgcaagaggc aagcagctgg
     1021 cgcagcacaa cgcgcatttt ttagtcgagt ttatggcgaa gctcagtgcc gaactggcgg
     1081 gggagaatga aggtgtcgat cacaaggtga tagatgcgtt ttcacccgcg ggataagcgc
     1141 gtggccctaa atggctgtaa ttatgttaac ctgtcggcca tctcagatgg ccggttaaat
     1201 ctatgcagga aaatatatca gtaaccgatt catacagcac cgggaatgcc gcacaggcaa
     1261 tgctggagaa actgctgcaa atttatgatg ttaaaacgct ggtggcgcag cttaatggtg
     1321 tgggtgagaa tcactggagc gcggcaattt taaaacgtgc gctggcgaat gactcggcat
     1381 ggcaccgttt aagtgagaaa gagttcgccc atctgcaaac gttgttaccc aaaccatcgg
     1441 cacatcatcc gcattatgcg tttcgcttta tcgatctatt tgccggaatt ggcggcatcc
     1501 gtcgcggttt tgaatcgatt ggcggacaat gcgtgtttac cagcgaatgg aacaaacatg
     1561 cggtacgcac ttataaagcc aaccattatt gcgatccggc gacgcatcat tttaatgaag
     1621 atatccgcga catcaccctc agccataaag aaggcgtgag tgatgaggcg gcggcggaac
     1681 atattcgtca acacattcct gaacacgatg ttttactggc cggtttccct tgtcagccat
     1741 tttcgctggc tggcgtatcg aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg
     1801 atacccaggg cacgctgttt tttgatgtgg tacgcattat cgacgcgcgt cgtccggcga
     1861 tgtttgtgct cgaaaacgtc aaaaacctga aaagtcacga ccagggtaaa acgttccgca
     1921 tcatcatgca gacgctggac gaactgggct atgacgtggc tgatgcagaa gataacgggc
     1981 cggacgatcc gaaaatcatc gatggtaaac attttctgcc gcagcaccgt gaacgcatcg
     2041 tgctggtggg ttttcgtcgc gatcttaatc tgaaagccga ttttactctg cgtgatatca
     2101 gcgaatgttt ccctgcacag cgagtgacgc tggcgcagct gttggacccg atggtcgagg
     2161 cgaaatatat cctgacgccg gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc
     2221 aggcgcgcgg taacggcttc ggttatggaa tggtttatcc gaacaatccg caaagcgtca
     2281 cgcgtacgct gtctgcgcgt tattacaaag atggcgcgga aattttaatc gatcgcggct
     2341 gggatatggc cacgggtgag aaagattttg acgatccgct gaatcagcaa catcgtccac
     2401 gtcggttaac gcctcgggaa tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga
     2461 aattccgcat tccggtttcg gacactcagg cctatcgcca gttcggtaac tcggtggtcg
     2521 tgccggtctt tgccgcggtg gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt
     2581 tgcgtcagca agaggcacaa catggccgac gttcacgata aggccactcg cagcaaaaat
     2641 atgcgcgcga ttgccacgcg tgatacggcg attgagaagc gcctcgccag tctgttaacc
     2701 gggcagggcc tggcgtttcg cgttcaggac gccagtctgc ccggaagccc ggattttgtc
     2761 gttgatgaat atcgctgcgt gatatttacc catggctgct tctggcatca tcatcactgc
     2821 tatctgttta aagtgcctgc gactcgaacc gagttctggc tggagaagat aggtaaaaat
     2881 gttgaacgcg atcgccgcga tatcagtcgt ttgcaggaac tcggctggcg cgtactgatt
     2941 gtctgggagt gtgcattacg tgggcgcgag aagctgacgg atgaagcgct taccgagcgt
     3001 ctggaagagt ggatctgcgg cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat
     3061 ttactcgctt gatgcgtcct gaataactgg cgcaactacg ggttttgccg ggaagagata
     3121 ttttcccaac gtgaccagta ccaccgcgaa gacaattacg ccgagcgcca gccattcaat
     3181 cttcgacagc gtctctccgc ccagtcccgt acccagcaag accgcgacca ccgggttaac
     3241 gtaggcgtag ctggtggcga gagccggact gacattacgg attaaataca tataagcgtt
     3301 gatggcgata atcgaaccaa acagcgccag atagccgacc gcaaggaagc ctgaaaggga
     3361 agggagcgcc gtcagttttt cacctgcaat catcgacgcg atcattaaca ccacgcctgc
     3421 cgccagcatc tcaatcgcac ccgccatcat ccctacaggt aaggtaatgc gcgagccata
     3481 aaccgagcca aacgcccagc taatcgagcc gattaaaatc agaatcgcgc cccacggatt
     3541 gccgcttaaa tttccaccgc tattgagcat gatgattccg gcaagcccaa tggcgatacc
     3601 cacccattcc agtttgcgcg ttttaatgcc aaacaggcgg ctgaagcacc agggtaaaga
     3661 ggggcacggt tgcaaccaca acggcggcga tgccggaagg aacattttga tgctcggcaa
     3721 ccgtcaccat gccattaccg acagccagca ataacaggcc aatcagcgcg gcattgagca
     3781 gcggacgtag cggggggagc ttgtgtccgc gcagtagcaa aaatgccatc aataaaatac
     3841 cggctgccag gaatcgaacg cctgccatca ttaacggagg ccagctttcc acgccaatcc
     3901 gaatgacaaa ataggttgag ccccaaatga tatacaacgc aaacagcgcg ccaaaaagcg
     3961 gtaacaactg gcggaaacgc ataatccctc acggtggaaa taaaaaggtg gttcatagta
     4021 aacgtgaaaa tcattctgct ggcgagagat ataattgcac ttgattgtaa aaaaaatgtt
     4081 gacctgtgaa gcaagtatca gagctgccgt ttttgcttca tacttacacc cttcaacaat
     4141 aaaaatgaga gggaatgctt ttggccggga gtagtttact gacgttgctc gatgatatcg
     4201 ccacactact ggacgatatc tccgtgatgg gcaaactggc ggcgaagaaa accgccggtg
     4261 tattagggga tgacttatcg ctcaatgcgc aacaagtttc aggcgtgcgg gccaaccggg
     4321 aacttcccgt ggtctggggc gtggcgaaag gatcgctgat taataaagtg attctggtgc
     4381 cgctggcgct gatcatcagt gcgtttatcc cgtgggcgat tacgcctctg ttgatgattg
     4441 gtggcgcgtt tctctgcttt gaaggagtag agaaagtgct gcatatgctg gaggcgcgta
     4501 aacataaaga agatccggcg cagagccagc agcgtctgga gaagctggcg gcgcaggatc
     4561 cgctgaagtt tgaaaaggac aaaataaaag gggcgattcg taccgatttt atattgtctg
     4621 ccgaaatcgt cgccatcacg ctggggattg tggccgaagc gccgttgctt aatcaggtgc
     4681 tggtgctttc aggcatcgcg ctggtagtga ccgtgggcgt ctacggtctg gtaggggtta
     4741 tcgttaagat tgatgacctg gggtattggc tggcggaaaa atccagcgcg ctgatgcagg
     4801 cattaggtaa agggttattg atcattgcgc cctggttgat gaaggcgtta tcgattgtcg
     4861 gcacgctggc gatgttcctc gtcggcggcg gtattgtggt acatggtatt gcgccgctgc
     4921 atcacgccat tgaacatttc gccgggcagc aaagtgcagt ggtggcgatg atattaccga
     4981 ctgttttaaa tctgattctt ggatttatca tcggcggcat cgtggtgctg ggagtgaaag
     5041 ccgtagcgaa aatgcgcggt caggcacatt aatactcgta caggctatta tgcaaaattc
     5101 gccatcttca actaaggtga acaagtttca ctcgaaaaaa ggaggcgagt atgagcttta
     5161 tggttagtga ggaagttacg gttaaagagg gcggcccgcg gatgattgtc actggatact
     5221 ccagcggtat ggttgagtgt cgttggtatg acggttacgg ggtcaagcgg gaagcttttc
     5281 atgaaaccga gcttgttccg ggggagggga gtcgttctgc ggaagaagtt tgaaagggta
     5341 tcattaacac caggccggat cacttccggc ctggtgtgcc aatgcctgca atgaatgata
     5401 gtcgcgtctt atcatgccac cattataaaa ttatcgctgt tttgcggtac gctatcgcca
     5461 ggttatgcca gaatcataaa aaagcaggtt gggagtcgtc agggtgcagc acgagacaaa
     5521 aatggaaaac cagagctggt tgaaaaaact cgcacgccgc ctggggcctg gtcatgtcgt
     5581 taatctctgc tttatcgtgg tattgctttt ttccaccttg ctgacctggc gtgaagtggt
     5641 agtgctggaa gatgcctata tctccagcca gcgtaatcat ctggaaaacg tagccaacgc
     5701 gctcgataag catttgcagt ataacgtcga caaactgatc tttttgcgta atggcatgcg
     5761 cgaagctctc gtagcgccac tggatttcac ttcactgcgt aatgctgtaa ccgagttcga
     5821 acagcatcgc gacgagcacg cctggcaaat tgaactcaac cgacgacgca ccctgtcagt
     5881 caatggcgta tcggatgcat tagtcagcga ggggaatctc ctgtctcgcg aaaatgaaag
     5941 cctcgacaat gaaattaccg ctgcactgga agttggttac ttgctgcgac tggcgcacaa
     6001 cacctcgtcg atggttgaac aggcgatgta tgtctcgcgt gccggatttt acgtttcgac
     6061 gcagccgacc ttgtttacgc gcaatgtacc aacgcgttat tacggctatg tcacccaacc
     6121 ctggtttatc ggccattcgc aacgagaaaa tcgtcaccgt gcggtacgct ggtttacttc
     6181 gcaaccggaa cacgccagca atactgaacc gcaggttacc gtcagtgttc cggtagacag
     6241 taataactac tggtatggcg tgctggggat gagtattccc gtgcgtacca tgcagcaatt
     6301 tttaagaaac gccatcgata aaaacctcga tggtgagtat cagctctatg acagtaagct
     6361 gagatttttg acctcttcca atcctgacca tccaacaggg aatatttttg atcctcgtga
     6421 actggccttg ctggcgcagg cgatggaaca tgacacgcgg ggcggcattc gtatggacag
     6481 tcgctatgtt agctgggaac gtctggacca tttcgacggt gtgctggtgc gcgtccatac
     6541 gctaagcgaa ggcgtgcgcg gcgatttcgg cagtatcagc attgcattaa ccctgctgtg
     6601 ggcgctcttt accaccatgt tactcatctc ctggtatgtg attcgccgga tggttagcaa
     6661 catgtatgtt ctgcaaagct cgttgcagtg gcaggcgtgg cacgacacct taacccgttt
     6721 atataatcgt ggcgcactgt ttgaaaaagc ccgtccgctc gcgaaattgt gtcagacgca
     6781 ccaacatcct ttttctgtca ttcaggtcga ccttgaccat tttaaagcga ttaatgaccg
     6841 ctttggtcat caggcgggcg accgtgttct ttctcatgct gccggattaa ttagcagttc
     6901 cttgcgtgcg caggacgttg ccgggcgggt cggtggtgag gagttttgtg tgattctgcc
     6961 aggcgcgagt ctgacggagg ctgcggaagt cgcagaacgt attcgcctga agttaaatga
     7021 aaaagagatg ttgattgcta agagtacgac gatacgcatc agtgcctcgt tgggggtaag
     7081 tagcagcgag gaaaccggtg attatgattt tgaacaactc cagtcactgg ctgaccgtcg
     7141 gctttatctc gctaaacagg ccgggcgtaa tcgggtatgc gcgagcgata acgcttaacg
     7201 agcggagaaa aaatggtcca gcccttcacg ccagccttcc ggtccttcac gctgcgttcg
     7261 ccagacgcgg gtcggatcct catcatgcag atgcacccct tcacggttta gccctttcac
     7321 aatcaccgcg taatccatta cctccagtaa gggcgcatcg tttggcccat cgcccaggcc
     7381 aagtgtggtt gggcgtttgc ctgataattg ttgataggtc gcgataatcc agttggcggc
     7441 ctgatctttt ccggcagagg catccaggac gtgccagaag cgcgcaccct gcataaactg
     7501 taagcccagt tcgttcagac gagcggtaaa ttgtgccata cgctcgtcac tgtcgcgcca
     7561 gattagcgtt accgacgcct catgcagctg cgtcagcgcc gcctggctac ggcttaatcc
     7621 cgtccattcg gcgatggttg catcgtcgac atcatcgaaa gtcgtgaatt taaaatgttc
     7681 tttctcgcgt agcgtattta aaacctggct gatttcgcca tggctaatac ctgagatgat
     7741 gcgtggaaaa ccgtctatat cctgccattg ttcagcaagc tggatcactg cgccattttc
     7801 ggcaatcagt ggtaaccctt gtagccccaa cgttttttgc aagtacagca tttccgctga
     7861 agttttactg ctacagagaa tgacgggaac attcgcttcg cgtaaacggc tgagccagag
     7921 ggcagtcggt tgccagtcat aactatgact gtccagcagg gtgccatcaa gatcgctaaa
     7981 aaccagtagt ggttgttgaa ttgaaaacat ggtcgtgcag ttctcccctt accaggatga
     8041 ttctcaaaac agcacaaata atcaaagatg attttttcgg gagcgatccc gttgacgcca
     8101 gagccaccaa ggggtgacgc acgtcacatt tctattcata agtagcgtta attattcata
     8161 tcgcgaatat tttcttgtca gcgaaaaaaa ttgcggataa ggtgatgaac acatcagatt
     8221 tcctggtgta acgaattttt taagtgcttc ttgcttaagc aagtttcatc ccgaccccct
     8281 cagggtcggg atttttttat tgtgcattca acgattcact tcagagacgc tgaagtcatg
     8341 aatatccagt tcgaaggcat ccgccaattc acgccatgta tgatattcac gtccatcgac
     8401 gctaacgtgt tcagaatcgt tctggctgcg atgaacttcg ctttcgctaa tttcattgat
     8461 cgccgccagc agggcatcga catcgacgct gacctcacgt tttgcggtat cgctgtactc
     8521 ttttgcggtt ttcatcatct ttcctcgcaa ccgttactct gttgttgccg ccagtcgtta
     8581 agtccgcctg gcatctctta agtatagacc gtcgagaaaa tcagcactcg cgcgcgcctg
     8641 gagctgccag aaaaacgaaa ttgttctaca ctggcacaaa gccacaggag gaaaacgatg
     8701 aaggtgaatg atcgggtaac agtcaaaacg gatggcggtc cgcgtcgtcc tggcgtggta
     8761 ctggcagttg aggagtttag tgaaggcaca atgtacctgg tttcgctgga agactacccg
     8821 ctcggcatct ggttctttaa tgaagcaggg catcaggacg gtatctttgt ggagaaagca
     8881 gagtaatctt attcagcctg actggtggga aaccaccagt cagaatgtgt tagcgcatgt
     8941 tgacaaaaat accattagtc acattatccg tcagtcggac gacatggtag ataacctgtt
     9001 tattatgtgt tttgatct
//
LOCUS       DAZOYL010000158         8982 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZOYL010000158 DAZOYL010000000
VERSION     DAZOYL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8982
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.158"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..1091
                     /gene="dacC"
                     /locus_tag="ACHUOY_002680"
                     /pseudo
     CDS             1..1091
                     /gene="dacC"
                     /locus_tag="ACHUOY_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1138..1896)
                     /gene="deoR"
                     /locus_tag="ACHUOY_002681"
     CDS             complement(1138..1896)
                     /gene="deoR"
                     /locus_tag="ACHUOY_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR0683425.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1954..2550)
                     /gene="ybjG"
                     /locus_tag="ACHUOY_002682"
     CDS             complement(1954..2550)
                     /gene="ybjG"
                     /locus_tag="ACHUOY_002682"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0683426.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2835..4062
                     /gene="mdfA"
                     /locus_tag="ACHUOY_002683"
                     /pseudo
     CDS             2835..4062
                     /gene="mdfA"
                     /locus_tag="ACHUOY_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4103..4387)
                     /gene="ybjH"
                     /locus_tag="ACHUOY_002684"
     CDS             complement(4103..4387)
                     /gene="ybjH"
                     /locus_tag="ACHUOY_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR0683427.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4473..5288)
                     /gene="ybjI"
                     /locus_tag="ACHUOY_002685"
     CDS             complement(4473..5288)
                     /gene="ybjI"
                     /locus_tag="ACHUOY_002685"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR0683428.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5288..6496)
                     /locus_tag="ACHUOY_002686"
     CDS             complement(5288..6496)
                     /locus_tag="ACHUOY_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0683429.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6580..7116
                     /gene="rcdA"
                     /locus_tag="ACHUOY_002687"
     CDS             6580..7116
                     /gene="rcdA"
                     /locus_tag="ACHUOY_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR0683430.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7291..8976)
                     /locus_tag="ACHUOY_002688"
     CDS             complement(7291..8976)
                     /locus_tag="ACHUOY_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR0683431.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
BASE COUNT         2338 a         2258 c         2293 g         2093 t
ORIGIN      
        1 tgcgcgtgca tggattttaa tggattacgc cagcggtaaa gtgctggcag aaggcaacgc
       61 ggatgagaaa ctggatcccg cgagcctgac taaaatcatg accagctatg tggttgggca
      121 ggcgcttaag gccgataaga ttaaactcac cgatatggtg acggtcggta aagatgcctg
      181 ggcgacggga aatccggcac tgcgtggttc atcggtaatg ttcctcaaac cgggcgatca
      241 ggtttcggtg gcagacttga acaaaggtgt gattatccag tccggtaatg acgcctgtat
      301 tgcgctggcc gattacgttg ccgggagcca ggagtcattt attggtttga tgaatggtta
      361 tgccaaaaaa actgggtctg accaacacta ccttccagac ggtgcacggt ctggatgcgc
      421 cggggcagtt cagtaccgcg cgcgatatgg cattgctggg taaagcattg atccacgatg
      481 tgccggaaga gtacgccatt cataaagaga aagagttcac cttcaacaaa attcgtcagc
      541 ctaaccgtaa ccgtctgctg tggagcagca atctgaatgt tgatggcatg aagacaggaa
      601 ccacggcagg tgcgggatat aatctggttg cttcggctac ccagggtgat atgcgtttaa
      661 tctccgtagt gctgggggcg aaaactgacc gtatccgttt taatgagtct gagaaattat
      721 tgacctgggg tttccgcttc tttgaaactg tgacgccaat taaacctgat gccacctttg
      781 tgactcagcg cgtctggttt ggtgataaga gcgaagtgaa tctcggggca ggcgaagcgg
      841 gctccgtgac cataccgcgt gggcagctga aaaacctgaa ggcgagctac acgttaacgg
      901 aaccgcagct taccgcaccg ctgaaaaaag gtcaggttgt cgggaccatt gatttccagc
      961 ttaacggtaa atccattgag cagcgtccgc tgatcgtgat ggaaaatgtg gaagagggcg
     1021 gattctttgg tcggatgtgg gatttcgtga tgatgaaatt ccatcagtgg ttcggcagct
     1081 ggttctctta atcttctgat aaccggatgg cgcgaaacgt catccggtta tacgtcatta
     1141 atacatcaac ttaatacggt gcgtctgcgc gtacttcaca tactcatctt ccgggcaaca
     1201 atcactcacc acaatatcaa agcgtttcag gtcacccatg cgcgccggac gcaccttgcc
     1261 aaatttactg tggtcgacaa ccagcacatg cttttgcgcc atcgacatgg cccagtgttt
     1321 taccggcaac tcttcaagat taaaacaggt agcgccttta ctgacatgca cgcccgccgc
     1381 ggaataaaaa gcgatatccg ggcaaaaatt attcaacgtt tgctgaaaat cgatggggtt
     1441 gaaaatggcg ttgctggcgt gaaattcacc accgcacaga aatgcgcggc aatggggttt
     1501 ctctttcagc gccagaaagg tatttagcga ataacaaacg gcggtaaaag ggatttcatt
     1561 atcaatcgct tcaataatcc acggcgtggt ggtgccacag tcaaaaaaga gggtctgatc
     1621 gggttctacc agtgtcgcag ccagttttgc cgcccggcgt ttttcttcca ccaggcggga
     1681 tttttgatcg cttaacaggt aatggctggc actgcgcggt tccagaacaa tatagccgcc
     1741 gagcaaaacg acgggcgcac tgtggttgtt cagatcgcga cgaatcgtca tctccgaaac
     1801 cccaagcagg gcggcggcgt ctttaagatg taacttatcg ctgcgtttta attcttgcag
     1861 cagctgcccg atacgctctt cgcgacgtgt ttccataatc cctctgaata gttattgaag
     1921 cgagccgctc aatactacac tttttagcag agatcagtca cgcacccagc ctttgcggat
     1981 cggtaatgca aaacagacgc gataccacga ttgcagacgt tgatagagtt tatgccccat
     2041 cgcttgccag ataatctggg cactaaggca gccaatcata cctgccagca atccaccgag
     2101 catatccagc ggccagtgga cgccaagata aacgcgcgac caggcaatga cgacggccag
     2161 aaccattaaa agtgagccgg accacaggcg atgccagcat aaaaatgcca gtgcaaaggt
     2221 gaaaatcacc gtaccgtgat cgctcgggaa tgagtcatcc gccgcatgat gcaggaagtt
     2281 atagccgata ttttcgacaa agggtcggtc gtgcggaaaa agatgtccca tcgtccagga
     2341 cacaaacagg ctgaccgcca gcgcgatagc gatttttatc accagttgcc gttgtgctgt
     2401 aagcccccac aaccaaagta ccgcggccag caacggcacc acggtaatca aatctttagc
     2461 aataaaaatc gccaacgaga tcatccacgg agccgagtct ggcgtcgcgt taataagaga
     2521 gaatagagag agattcaaat tttccagcat aacttcccga cgcaaagtga ttaaaaggga
     2581 gccaatacag gcaagtcgtt gagaataaag tgcaggttaa actgggtaaa gcggcatcgt
     2641 cttatttccc tcaagcggcc tgtttacggt gggtgattgt aacgggcata ggttaaataa
     2701 aacttaaaga aagcgtagct atactcgcaa taatgtaaga atgtgcttaa ccgtggtttc
     2761 agctacaaaa ttcgctttct cgttagctgc gcttttatta aactctgcgc gattattatt
     2821 ggcgaagaaa ttgcatgcaa aataaattag ctttcggtgc caggcttgga cgtcaggcgt
     2881 tacttttccc tctctgtctg gtgctttacg aattttcaac ctatatcggc aacgatatga
     2941 tccaacccgg tatgttggcc gtggtggaac aatatcaggc gggcattgat tgggttccta
     3001 cttcgatgac cgcgtatctg gcgggcggga tgtttttaca atggctcctg gggccgctgt
     3061 cggatcgtat tggtcgccgt ccggtgatgc tggcgggtgt ggtgtggttt atcgtcacct
     3121 gtctggcaat attactggcg caaaacattg aacaattcac cctgttgcgc ttcttgcagg
     3181 gcataagcct ctgtttcatt ggcgctgtgg gatacgccgc aattcaggaa tccttcgaag
     3241 aggcggtttg tatcaagatc accgcgctga tggcgaacgt ggcgctgatt gctccgctac
     3301 ttggtccgct ggtgggcgcg gcgtggatcc atgtgctgcc ctgggagggg atgtttgttt
     3361 tgtttgccgc attggcagcg atctcctttt tcggtctgca acgagctatg cctgaaaccg
     3421 ccacgcgtat aggcgagaaa ctgtcactga aagaactcgg tcgtgactat aagctggtgc
     3481 tgaagaacgg ccgctttgtg gcgggggcgc tggcgctggg attcgttagc ctgccattgc
     3541 tggcgtggat cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg
     3601 aatatggctt gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag
     3661 cgcgtctgac ctcgcgccgc accgtacgtg ataattatgg gcggctggcc gattatgatt
     3721 ggtctgttgg tcgctgctgc ggcaacggtt atctcatcgc acgcgtattt atggatgact
     3781 gccgggttaa gtatttatgc tttcggtatt ggtctggcga atgcgggact ggtgcgatta
     3841 accctgtttg ccagcgatat gagtaaaggt acggtttctg cggcgatggg aatgctgcaa
     3901 atgctgatct ttaccgttgg tattgaaatc agcaaacatg cctggctgaa cgggggcaac
     3961 ggacagttta atctcttcaa ccttgtcaac ggaattttgt ggctgtcgct gatggttatc
     4021 tttttaaaag ataaacagat gggaaattct cacgaagggt aaaaaaatgc ctgactgctt
     4081 tgtgcgatca ggcattctcg aattaatggt gatggtcgtc aatctggtgt tcgataacca
     4141 tcccttcacc tacgctggca agatggcgag cataaggatg cgggcggtaa gccggagctg
     4201 gcgcaggagc cacatagacg gtttgcggca cagtcgcgac gctgaccgct tgtggaacgc
     4261 tgactgaatc aggaactaca accactttgt atccactcgg cacatcgacg gtgacacttt
     4321 gcgccatcgc cacaccagta aagcccatta aaagcgcgcc caacagtaga caatttttca
     4381 taatcatttc gcctgaaata atgcagcggg ttaatttccg cgaattatgc agccttcatt
     4441 caggtattgt gttgccgggc gtgctgccgt ttttattggt caaatggcgc ttcgtgttta
     4501 agcactttat cgatcacatc cagtacgcct tcacggttat tggagcctgc ccggtatttt
     4561 gccgctgcga cgaccgcgct gccggcattt tccattgcaa aactaaagcc tgcctgacgc
     4621 agcatctcaa tatcgttacc gccatcgcca aagaccacca cttcgctgtc gtctattccc
     4681 cataatttct gcagttggcg aaggccattg gctttatgta cgccggggat aatcagatcg
     4741 atgctgccgt tgccggtgtg gactggcacc ataatatcgc cgatggcctc atgtaatgct
     4801 ttttgtactt gtggaatcag ttcatcggaa agattcaggc caaacttaaa gaaaatatct
     4861 tctaagttgt caaagttatc gacgtattcc agacggtgat aatacatttc tgccaccgtt
     4921 ttcatggcat cgtcatattt tttgagcgta taggcgctat tttttccgca ggcaataatt
     4981 tccacttccg ggcgcgtcag caaatgttcc acgacagtag taaaggcatc cttcgacagc
     5041 tcgccattaa aaacatcttt gccttcactc actacccagc cgccgttttc agccacaaag
     5101 gcaatttcat tagcaatttc agggaagaaa gagagcaact gataatattg attcccgctg
     5161 gcgaccacaa agcgaatgcc ttgtgctttc atttgctgat actgagccat aaaccgctca
     5221 cggttatagg ttttttgatc gcttaagaaa gtaccatcca tgtctaccgc aattaattta
     5281 atgctcatca actattctcc atcgccgtcc gcgttttggt atcgggtttg gcgacggctt
     5341 tcgcgacaat agccgcgaga ataaccagcg ccagtacaac cagcattgca ctacgtaatc
     5401 cataatgttc gccgagatag cccagcagcg gcggcccgac gaggaaagcc agataaccgg
     5461 tcgttgctac cacactgacg cgggttggtg catcggggcc ggtatcgctg gcggcagaaa
     5521 tggtcagcgg gaagcccagc gaggcaccca gtccccagag tacaacagac accccagcga
     5581 cccaggcgct atcgacaaaa ataatcagcc caatacccaa cgcccccatt agtgcactgg
     5641 cccgaaccac ggcaacgcga ctgtaacggt cgatgaacca accgccagtg aagcgtccaa
     5701 cggtcatccc tagggtaaaa ccggcataaa tcagcgagcc ggaagtaggg ctaaaaccgt
     5761 gaccgtcaac cattaataag ggtaaccagt cgttggcaga accttcggca aaggccatcg
     5821 ccagcactac aacaccaatc aacagcaact ggatatcgcg ataaaaagga atgccttttt
     5881 cgccatgctg ggtgccatcg gcagcatttt tgcccgtacc gtcagggatt gcctgaatgg
     5941 cgatataaat aggcgcgata cctaccagcg ccgccaataa aatgtgcacc gttgccggaa
     6001 cgccaaaggc cgtcagtgcc atcccgacac cagcgcctgc cagcgtgcct aggctataaa
     6061 aaccgtgcat catcggcaaa accgttttat tcatttctcg ctcaacggcg gcaccttcaa
     6121 cgtttatcgc cacttccgca gaaccaaaac ttgccccaaa gacgccgaga ccaacggcaa
     6181 agagcagggg cgatgtcagc cagagtgcca gacttaatat catcatcccg atcaatgcgc
     6241 aggacatcgt gaccaggatg acattacgtg tcccaaagcg tttcactaac cacgccgagc
     6301 agagaatacc gctcatcgaa ccgatcgaca gaccaaagag aacaccgccc atttcagcga
     6361 tcgagacaga gagaatatcg cggatagcag gcgtacgggt tgcccaggac gccattaaca
     6421 ggcctggcaa aaagaagaac ataaacagcg cccaggttcg gcgtttcaat gcattacgtg
     6481 aagaatttac ggtcatagat cacgtcaaaa taagaagagg gaagacaaca ttagcaaggt
     6541 tgtgtacatt tgtacacaat tgcagacaga ggaaatgaca tgcgtcgcgc taacgatccg
     6601 caacggcgag aaaaaattat ccaggccacg ctggaggcgg tgaaacttta cggaatacat
     6661 gctgttacgc accgcaaaat tgctaccctt gccggggtac cgttgggatc gatgacctac
     6721 tatttttcag gaattgatga gttgttgctg gaggcgttca gcagttttac tgagattatg
     6781 tcccggcaat atcaggcatt ttttagcgat gttagtgatg ctcaaggcgc atgccaggct
     6841 atcaccgata tgatctacag ctcacaggtt gcaacgccgg ataatatgga gctgatgtac
     6901 cagctctacg cgctggctag ccgaaaaccg ctattaaaaa cggtaatgca aaactggatg
     6961 cagcgcagtc agcaaacgct cgaacaatgg tttgaacccg gaaccgcccg cgcgcttgat
     7021 gcgtttattg aggggatgac gctgcatttt gtcaccgacc gtaagccgct atcgcgcgag
     7081 gagattttga ggatggttga gagggttgca gggtagtaga taaattttag ataacaaaaa
     7141 acccatcaac cttgaaccga aatggcgggg ttgatgggct ccacaaaatg gggacatcaa
     7201 agaaaagcag tggcactaat taagactgat gccctgcgga aaagttctgc ggttgtgcaa
     7261 aaaaatttca ttttcagggc aacttcagtt ttatcctaat cctggccata ccatgacgat
     7321 gattgtccct gccagcgtca gcaggacgtt ggcgattgcg taggtgcccg catagcccag
     7381 cgcagggatg ttactgcgag ctgtatcact gatgatctcc attgccggcg cgcaggtgcg
     7441 tgcgcccatc attgcgccga acaacagtgc gcggttcatt cgcaatacat aagcaccgaa
     7501 caagaaacag ataaccacgg gcacaagact gacaattaat ccggcaatca acatctgacc
     7561 gccaatcgcg cccaggccgt tattaatacc gctaccggcg ctcagaccaa cgcctgccat
     7621 aaacaccatc aagccgaact ctttcaccat gcttaatgca ccttgcggaa tgtaaccgaa
     7681 ggtcgggtgg ttagcacgca taaagcccag cataattccg gcgaataaca acccggcagc
     7741 gttccccatg ccgaaactga atgtgctgaa ctggaaggtg atcatcccga tcatcagccc
     7801 aataacaaag aaggcgcaaa atgccagcag gtcagtgacc tggctgtgaa tcgagataaa
     7861 gccgatgcga tcggcgatgg tttttacgcg acgggcatca ccgctgactt gtaaaacgtc
     7921 acctttgtta agcacgacgt tgtcatctat cggcatctca atctggctac gaatgacgcg
     7981 gttaaggaag caaccgtgat cggtcaactt cagttgtgcg agacgtttac ctacagcgtt
     8041 atggttttta acgaccactt cttcagtgac gatacgcatg tcgagaaggt cacgatcgaa
     8101 aacttcttta ccgttacgga agctgggatc gagtcgggca tgggcgtcgg gatagcctac
     8161 caacgctatt tcatcgccca tttgtagcac ggcatcaccg tctggatttg ccagaatccc
     8221 gttacgtcga atacgttcaa tgtagcagcc ggtttgtcga taaataccca gttcacgcag
     8281 atttttgccg tcggtccagg ccaccagctc cgggccgacg cgataggcgc ggatcaccgg
     8341 taaataaacc ttacggttgg catcagtgtc caggccacgt tcgcgggcga tttgctgggc
     8401 gctggtctgt aagtcctgat gctgcaattt cggcaagtaa cgcgcaccaa caatcaaact
     8461 caccagaccg attaaatagg ttaaggcata cccgaggctc agattatcca gtgccagtga
     8521 gagctgcctg ctttccatgc cggaatgacg cagtgtatcg ccagcaccga ccagaaccgg
     8581 tgtcgacgtc atagagcctg ctaacatacc ggccgtcagg ccaatatccc agccaaacag
     8641 cttacctaac cctaaggcga tcaccagcgc actgccaacc atcaccagtg ctaacattag
     8701 gtaatttttc ccatcgcgaa aaaaaatgga aaaaaagttc ggtccggctt cgaccccgac
     8761 gcagaaaata aacagcataa agccaagatt aagcgcatcg gtgttaatgc tgaaatgttg
     8821 ttggcctaat aacagcgata cgactaaaac gccaatggaa ttacccagtt ggatcgaacc
     8881 aagtcgtaac tttccgagac atagcccaag cgtgaggacc acaaataata acagaatgta
     8941 attcccattt aacaattcgg cgacgtttat attcacggag gc
//
LOCUS       DAZOYL010000159         8917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZOYL010000159 DAZOYL010000000
VERSION     DAZOYL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.159"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            235..2628
                     /gene="malP"
                     /locus_tag="ACHUOY_002689"
     CDS             235..2628
                     /gene="malP"
                     /locus_tag="ACHUOY_002689"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR0683432.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2638..4722
                     /gene="malQ"
                     /locus_tag="ACHUOY_002690"
     CDS             2638..4722
                     /gene="malQ"
                     /locus_tag="ACHUOY_002690"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR0683433.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4767..6083)
                     /gene="gntT"
                     /locus_tag="ACHUOY_002691"
     CDS             complement(4767..6083)
                     /gene="gntT"
                     /locus_tag="ACHUOY_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0683434.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6444..7019)
                     /gene="nfuA"
                     /locus_tag="ACHUOY_002692"
     CDS             complement(6444..7019)
                     /gene="nfuA"
                     /locus_tag="ACHUOY_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR0683435.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7078..7761)
                     /gene="gntX"
                     /locus_tag="ACHUOY_002693"
     CDS             complement(7078..7761)
                     /gene="gntX"
                     /locus_tag="ACHUOY_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR0683436.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7799..8569
                     /gene="bioH"
                     /locus_tag="ACHUOY_002694"
     CDS             7799..8569
                     /gene="bioH"
                     /locus_tag="ACHUOY_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR0683437.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(8598..>8917)
                     /locus_tag="ACHUOY_002695"
     CDS             complement(8598..>8917)
                     /locus_tag="ACHUOY_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0683438.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         2194 a         2228 c         2489 g         2006 t
ORIGIN      
        1 gaaaatacgc aacggccatt ttttgcactt agatacagat tttctgcgct gtattgcatt
       61 gatttgctgc taatcctgtg gtttgcacta gctttaagtg gttgagatca catttccttg
      121 ctcatccccg caactcctcc ctgcctaatc ccccgcagga tgaggaaggt caacatcgag
      181 cctggcaaac tagcgataac gttgtgttga aaatctaaga aaagtggaac tcctatgtca
      241 caacctattt ttaacgataa gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat
      301 ggcttaaatt ctgcggctga aatgactcct cgccagtggt ggctagcagt gagtgaagca
      361 ctggccgaaa tgctgcgtgc tcagccattc gccaagccgg tggcgaatca gcgacatgtt
      421 aactacatct caatggagtt tttgattggt cgcctgacgg gcaacaacct gttgaatctc
      481 ggctggtatc aggatgtaca ggattcgttg aaggcttatg acatcaatct gacggacctg
      541 ctggaagaag agatcgaccc ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc
      601 ttcctcgact caatggcaac tgtcggtcag tctgcgacgg gttacggtct gaactatcaa
      661 tatggtttgt tccgccagtc ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg
      721 catcgcagta actacccgtg gttccgccac aacgaagcac tggatgtgca ggtagggatt
      781 ggcggtaaag tgacgaaaga cggacgctgg gagccggagt ttaccattac cggtcaagcg
      841 tgggatctcc ccgttgtcgg ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag
      901 gcgacgcacg cgcatccgtt tgatctgact aaatttaacg acggtgattt cctgcgtgcc
      961 gaacagcagg gcatcaatgc ggaaaaactg accaaagttc tctatccaaa cgacaaccat
     1021 actgccggta aaaagctgcg cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg
     1081 gatattttgc gtcgccatca tctggcgggg cgtaaactgc acgaactggc ggattacgaa
     1141 gttattcagc tgaacgatac ccacccaact atcgcgattc cagaactgct gcgcgtgctg
     1201 atcgatgagc accagatgag ctgggatgac gcttgggcca ttaccagcaa aactttcgct
     1261 tacaccaacc ataccctgat gccagaagcg ctggaacgct gggatgtgaa actggtgaaa
     1321 ggcttactgc cgcgccatat gcagattatt aacgaaatta atactcgctt taaaacgctg
     1381 gtggagaaaa cctggccggg cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat
     1441 aaacaagtgc atatggcgaa cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg
     1501 gcgctgcact cggatctggt ggtgaaagat ctgttcccgg aatatcacca gctatggccg
     1561 aacaaattcc ataacgtcac caacggtatt accccacgtc gctggatcaa acagtgcaac
     1621 cctgcactgg cggctctgtt ggataaatca ctgcaaaaag agtgggctaa cgatctcgat
     1681 cagctgatca atctggaaaa attcgctgat gatgcgaaat tccgtcagca atatcgcgag
     1741 atcaagcagg cgaataaagt ccgtctggcg gagtttgtga aagttcgtac cggtattgag
     1801 atcaatccac aggcgatttt cgatattcag atcaaacgtc tgcatgagta caaacgccag
     1861 cacctgaatc tgctgcatat tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct
     1921 gatcgcgtac cgcgcgtctt cctcttcggc gcgaaagcgg caccgggcta ctacctggcg
     1981 aagaatatta tctttgcgat caacaaagtg gctgacgtga tcaacaacga tccactggtt
     2041 ggcgataagt tgaaggtggt gttcctgccg gattattgcg tttcggcggc ggaaaaactg
     2101 atcccggcgg cggatatctc cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc
     2161 ggcaatatga aactggcgct caatggtgcg cttactgtcg gtacgctgga tggtgcgaac
     2221 gttgaaatcg ccgagaaagt cggtgaagaa aatatcttta tttttggtca taccgtggaa
     2281 caagtgaagg caattctggc caaaggctac gacccggtga aatggcggaa gaaagataag
     2341 gtgctggacg cagtattgaa agagctggaa agcggtaaat acagcgacgg cgataagcat
     2401 gccttcgacc agatgctgca cagtatcggc aaacaaggcg gcgatccgta tctggtgatg
     2461 gcggatttcg cagcctatgt tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag
     2521 gaggcctgga ctcgcgcggc gatcctcaat accgcccgct gcggtatgtt tagctcggat
     2581 cgctctattc gcgattatca ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg
     2641 gaaagcaaac gtctggataa tgccgcgctg gcggcgggga ttagccccaa ttacatcaat
     2701 gcccacggta aaccgcagtc gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg
     2761 catcaacgta ccgccacgaa agtggcggta acgccagtcc cgaatgtcat ggtttatacc
     2821 agcggcaaaa aaatgccgat ggtggtggag ggcagcggcg aatatagctg gctgctgacc
     2881 accgaagaag gaacacagta caaaggccat gtaacggggg gcaaagcgtt caatctaccg
     2941 acgaagctgc cggaaggtta tcacacgctg acactcaccc aggacgacca gcgcgcgcat
     3001 tgccgggtga ttgtcgcccc gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa
     3061 aagctgtggg gtgcctgcgt tcagctttat acgctgcgat cggaaaaaaa ctggggtatt
     3121 ggggattttg gcgatctcaa agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc
     3181 atcggcctga acccgattca tgcgctctat ccggcaaatc cggagagtgc cagcccatac
     3241 agcccgtctt ctcgccgttg gctgaatgtg atttatatcg acgttaaagc cgtcgaagat
     3301 ttccatctta gcgaagaggc tcaggcctgg tggcagttgc cgaccacgca acagacgctg
     3361 caacaagcgc gggatgccga ctgggtcgat tactccacgg ttaccgccct aaaaatgaca
     3421 gcattacgaa tggcgtggaa aggtttcgcg caacgtgatg atgagcagat ggccgcgttt
     3481 cgccagtttg ttgcagatca aggcgacagc ctgttctggc aggcagcctt tgatgcgcta
     3541 catgcccagc aagtgaaaga ggacgaaatg cgctggggct ggcctgcatg gccagagatg
     3601 tatcagaacg tggattcacc agaagtgcgt cagttctgcg aagaacatcg taatgacgtc
     3661 gatttttatc tctggttgca gtggctggct tacagccagt ttgccgcctg ctgggagata
     3721 agccagggct atgaaatgcc gattggcttg tatcgtgatc tggcggttgg cgtagcggaa
     3781 ggtggggcgg aaacctggtg tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg
     3841 ccgccggata tcctcggccc gttggggcag aactggggat taccgccaat ggacccgcat
     3901 atcatcaccg cgcgtgccta tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac
     3961 tgcggcgcat tacgaattga ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat
     4021 ggcgagacgg cagatcaggg cgcgtatgtt cactatccgg tggatgatct gctctcgatt
     4081 ctggcactcg aaagtaaacg tcatcgctgt atggtgattg gtgaagatct cggtaccgta
     4141 ccggtagaga ttgtcggtaa gctgcgcagc agcggtgtgt actcctacaa agtgctctat
     4201 ttcgaaaacg accacgagaa gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg
     4261 gcggttgcgg cgacacatga cctgcctacg ctacgcggtt actgggagag cggggatcta
     4321 acgctgggca aaaccctggg gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag
     4381 gatcgcgaac tggcgaagca agggctgctg gatgcactgc ataaatatgg ttgtctgcca
     4441 aaacgtgccg ggcataaggc atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg
     4501 cagcgctaca ttgccgacag taacagtgct ctgttaggac tacagccgga agactggctg
     4561 gatatggccg aaccggtgaa tattcctggc accagttacc agtataaaaa ctggcgacgc
     4621 aagctttccg caacgcttga gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag
     4681 gatttggaca gacggcgcag agctgcagcg aagaagaagt agagtgcggt tgatgccgga
     4741 tgcgatgcat ccggcagtca gtgttatcaa atcaccatat tcagcagcag acagcctacc
     4801 agaccgcaca ccgagataat ggtttccagc atcgaccagg atttgatggt ctcgccgata
     4861 gtcaggttaa agtactcttt gaacagccag aagcccggat cgttcacatg agagaaaatc
     4921 acactaccgg aacccaccgc aataaccatc agctctgggc taacacccgt cgttgcaatc
     4981 agcggtgccg cgataccacc cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata
     5041 cgcagtacgg cagcaatcga ccaggccatc agcagcggag aaatgttggt ttcgtgcatc
     5101 atggaagcaa tgtatttgtc cacgccgctg tctaccagca cctgcttgaa cgcaccgcca
     5161 ccaccgatga tcaacagcat catcgcaatg attttgatgg aagaaaccag cgtgtcgtta
     5221 atctgatcca ttgaacgacc acggttcaga ccaaaggtga acatcgcaat cagcacggca
     5281 atcagcgttg ccattaccgg gtcaccgagg aactccgcta ccggcaggaa agcgtgacct
     5341 ttcggcagga tcatttcggc aatcgcacgc atcgccatca gcacaaccgg caccagagaa
     5401 gtccagacgc tgacgccaaa gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag
     5461 agaccttccg ggattggctt atcgatacct ttcaacacgc gagcgtaaac cggaccggcg
     5521 agaatcacgg tcgggattgc cagaatagta ccgtacagca gggttttacc catatcggca
     5581 ttgaaaatgg tggcaatcgc agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca
     5641 gacagtgcag ccgccattgg tacaccaaca tacagcagtg gaatattcgc ggaagccgcg
     5701 atggtaaaca ccagcggcag catcagcacg aagcccactt catagaacag ggcaaaacca
     5761 acggtaaaac cggttaacac caccgcccac tggatgcgct ttttaccaaa tttggcaatc
     5821 agcgtggtgg cgatacgttg tgcgccaccg cagtctgcca gcattttgcc cagcattgcg
     5881 ccaaaaccca tgatcagggc aaggctaccg agcgtcccgc cgacaccgac tttgatggag
     5941 tcaataactt tatccagcgg cattccttgc attaatccaa cagcaagcgc cacgaggacg
     6001 agagcgatga agccgttcat tttgaagcgg atcatcagga gcaacaacaa gattacaccg
     6061 atagcaacaa tgactaatgg catgatttac ctggcctttc atttgttatg ggtaacgtca
     6121 attttctgac gacaaactct aattatccca atcgggaaca gagatattgc ggcaccacga
     6181 ctgataccca ctaaaactaa ttattgtagt cagatgtcag gagtatgttt ggtacccatg
     6241 tgaatgatac gggtaacatt tggcgtttga gaatcaccag agcggggtaa atttaaatta
     6301 tgagaggttg gtcatattat cgcggggaaa cgaagggagg gatttgccaa agcaatgctg
     6361 tgccaacgtc tggcacatgt tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg
     6421 caatctacaa aagaggggat aacttagtag taggagtgtt cgccgcgctg gtgttcggtg
     6481 agatcgcgca cacctttcag ctccgggaat tcgttcagca gctgcttctc gatcccttct
     6541 ttcagcgtca catcgaccat ggaacaaccg ttacagccgc cgccaaattg cagaatggcg
     6601 taaccgtctt cggtgatttc catcagcgaa acgcgaccac cgtgaccagc aagctgtggg
     6661 ttgatctgcg actgcagcat atactccacg cgctccatca gcggtgcatc gtctgccact
     6721 ttacgcattt tggcgttcgg ggctttcagc gttaactgag aacccaactg gtcggtaaca
     6781 aagtcgatct ctgcatcttc caggtatggt gcgcttaact catcaacata cgcggtcagc
     6841 aggtcaaatt tcagggcagt gtcggtggct tccacggcat ccggcggaca ataagaaaca
     6901 ccacattcag cgttaggcgt gccagggtta atcacaaata cgcggatttg tgtcccttct
     6961 tcctgatttg ccagcagttt ggcaaagtgc gcttgtgcag catcggaaat acggatcata
     7021 gtaatggcct aatagttgac tattttagtt ggttataata cgcccatcat cgaggctcta
     7081 caaggttcga caaaggcacc agacctggac agtcgccgca ccattgcgta aaagcaactg
     7141 cgcaatctct gcgacggtac ttccggtggt aacgacatca tccacaatca ccatatggcg
     7201 accttgcacg ggcaattcaa gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc
     7261 actgagaaaa tgctgggtcg cagtggcccg tgtacgtgtg acggcttcgc tatcccattg
     7321 gcagtgcaac cagcgtgata acggctgaca cagcaaatcg ctctgattaa atccccgacg
     7381 ccagtgacgc cgctgccata acggaacgct gatgatgcga tccggcaatt gcaacccggt
     7441 ggtgcgacga gcgtgtaaga cttccaatag taacagacgt gacagggcgc tggcgatttc
     7501 actgcgccgg gaaaatttaa gctggtggat gagcagactt aacggcggcg catagtcggc
     7561 aaccgtgacc agtctttgcc agggcggcgg tttttgcagg cagcgaccgc agggaagatg
     7621 ggagtgtgtg gcgggtaatc cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg
     7681 tgaacagacc gaacaaatcc cccaatgacc taacgccagt ggcattcggc atagccagca
     7741 taatcccggt actgttagca tatgttcatc cttgtaagtc aaaagagaac aatagcggat
     7801 gaataacatt tggtggcaga ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg
     7861 atggggactg aatgccgaag tgtggcgttg cattgacgag gaacttagct cgcattttac
     7921 gctgcacctt gttgatctgc ccggcttcgg gcgcagccag ggatttggtg cgctgtcact
     7981 tgctgatatg gccgaagccg tgctgcaaca ggcacctgat aaagccattt ggttaggctg
     8041 gagtctgggc gggctggtgg caagccagat tgcgttaacc catcccgagc gtgttcaggc
     8101 gctggtcacc gtggcgtcgt caccttgttt tagtgctcgt gacgattggc cggggataaa
     8161 accggacgtg ctggcgggat ttcagcagca actcagtgat gattttcagc gtacagtgga
     8221 gcggttcctg gcgttacaaa ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt
     8281 gaagaaaacc gttctggcgt taccgatgcc ggaggttgac gtgcttaatg gcgggctgga
     8341 aatcctgaaa acggttgatc tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg
     8401 attgtatggc tatctcgatg gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact
     8461 ctggcctcgc agcaaatcat atatcttcgc caaagcggcc catgcgccgt ttatttcgca
     8521 tccggtcgag ttttgtcacc tgctggtggc gttgaagcag agggtgtagg caacttttga
     8581 aatggcgaga cagaaattta ctggctcgcc gcagccaact cttcttctga caccccggta
     8641 aagcgcatga tgtccgcaag agggactccg gattcaagca ttattttggc tatatgcagg
     8701 gctttggatt gttccccctc ctgccgcaat ctttccgcaa ttgtcattaa actctccttg
     8761 tgtttcggtg aacgttcggc aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg
     8821 gtttgcagta tgtaattaaa cagccctttg atttgtctgt cattagcgta tccactactt
     8881 aataagcagg ccatttgctc taccagcccc atcaggt
//
LOCUS       DAZOYL010000160         8881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZOYL010000160 DAZOYL010000000
VERSION     DAZOYL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.160"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            143..646
                     /locus_tag="ACHUOY_002696"
     CDS             143..646
                     /locus_tag="ACHUOY_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR0683439.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(725..976)
                     /gene="yecJ"
                     /locus_tag="ACHUOY_002697"
     CDS             complement(725..976)
                     /gene="yecJ"
                     /locus_tag="ACHUOY_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR0683440.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1091..1177)
                     /gene="azuC"
                     /locus_tag="ACHUOY_002698"
     CDS             complement(1091..1177)
                     /gene="azuC"
                     /locus_tag="ACHUOY_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR0683441.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1440..1763
                     /locus_tag="ACHUOY_002699"
     CDS             1440..1763
                     /locus_tag="ACHUOY_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR0683442.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1934..2431
                     /gene="ftnA"
                     /locus_tag="ACHUOY_002700"
     CDS             1934..2431
                     /gene="ftnA"
                     /locus_tag="ACHUOY_002700"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR0683443.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2469..2708)
                     /locus_tag="ACHUOY_002701"
     CDS             complement(2469..2708)
                     /locus_tag="ACHUOY_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR0683444.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            2899..4110
                     /gene="tyrP"
                     /locus_tag="ACHUOY_002702"
     CDS             2899..4110
                     /gene="tyrP"
                     /locus_tag="ACHUOY_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR0683445.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4172..4837)
                     /gene="yecA"
                     /locus_tag="ACHUOY_002703"
     CDS             complement(4172..4837)
                     /gene="yecA"
                     /locus_tag="ACHUOY_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR0683446.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5033..5119)
                     /locus_tag="ACHUOY_002704"
     tRNA            complement(5033..5119)
                     /locus_tag="ACHUOY_002704"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5083..5085),aa:Leu,seq:taa)
     gene            complement(5132..5205)
                     /locus_tag="ACHUOY_002705"
     tRNA            complement(5132..5205)
                     /locus_tag="ACHUOY_002705"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5171..5173),aa:Cys,seq:gca)
     gene            complement(5260..5335)
                     /locus_tag="ACHUOY_002706"
     tRNA            complement(5260..5335)
                     /locus_tag="ACHUOY_002706"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5300..5302),aa:Gly,seq:gcc)
     gene            complement(5487..6035)
                     /gene="pgsA"
                     /locus_tag="ACHUOY_002707"
     CDS             complement(5487..6035)
                     /gene="pgsA"
                     /locus_tag="ACHUOY_002707"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR0683447.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6092..7924)
                     /gene="uvrC"
                     /locus_tag="ACHUOY_002708"
     CDS             complement(6092..7924)
                     /gene="uvrC"
                     /locus_tag="ACHUOY_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR0683448.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7921..8577)
                     /gene="uvrY"
                     /locus_tag="ACHUOY_002709"
     CDS             complement(7921..8577)
                     /gene="uvrY"
                     /locus_tag="ACHUOY_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR0683449.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         2193 a         2244 c         2074 g         2370 t
ORIGIN      
        1 gttattctta tttcagatca tcgtcagaat tgactccacg atcacatttc ggaccggcag
       61 aaaggaatta ttctgcaaac agtaattatg gtgttttgat ttatcttgca cctctccact
      121 tctggatata aggatattag gtatggcaac cgctggaatg cttctcaaac tcaactctca
      181 aatgaaccgc gagttttacg catccaatct ctaccttcac ctgagtaact ggtgttctga
      241 acagagtctg aacggcaccg ccactttcct tcgcgcccag gcacagagta atgtgaccca
      301 aatgatgcgc atgtttaact ttatgaagag tgtcggcgct acccccatcg ttaaagccat
      361 tgatgttccc ggtgaaaaac tgaactctct ggaagaactg ttccaaaaaa cgatggaaga
      421 atacgagcaa cgttctagta cgttggcaca gttagccgat gaagcgaaag aactgaatga
      481 tgattcaacc gtcaatttcc tgcgcgatct ggaaaaagaa cagcagcatg atggtctgtt
      541 gctgcaaacc attcttgatg aagtgcgcag tgcgaaactt gcgggtatgt gccctgtgca
      601 gaccgaccaa catgttctga atgtcgtgtc acaccagctg cattgatcat catcggcgct
      661 aatgcattgc gccgatgaag gttttgagaa accgctgcct catctgtttg aagcagcggt
      721 ttttttaatg ggattcaccc tgtggggtaa acttgagttc aataagcgcg atggcttttt
      781 ggattgcccg catggtgacc gggtctgcgg cggcgggatg gttagtaaag tcgatattct
      841 tcagctgact ggacattttt tcacgaactt caacgggcgc gattacatcg agaacatcca
      901 gaatttgttt gataaccaac tggcaagcaa ccacatcaga aaccagttcc tgatcggcat
      961 tcagcggctg ggacatcgta aactcctgat agcattttga aagccgttat agtagcggct
     1021 tcacatcttc agcgatagtc acatccaccg tcatcaggac acaaaaaaac ctgccggagc
     1081 aggttttttg ttatcggaac atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt
     1141 tattgaacat acttttcagg attttgcgca gtttcatcgc ggcactccga ccatttgtta
     1201 tacaggtgtt attgtctttg cgcttataat atgacaacca tcacaaaaat caatctttat
     1261 gtgatacaaa tcacataaat acccctttaa tgttataaaa atgataatca aaaaacagcc
     1321 cccctatttc tgacacctac agatggcaag aaatagcgcc tgccaggcgt cttttccggc
     1381 cattgtcgca gcgctgtaac gcgtaaaata gtgctttctc ttactcttct ggctggacca
     1441 tgagacttct gattctgact ctttcattaa taacgctggc ggggtgtacg gttactcgcc
     1501 aagcccatgt gagcgaagtt gacgcggcaa caggtattgt acggttggtt tatgaccagg
     1561 cttttttgca gcatgcccat accgatcgtt atgtcagtcg cgggatcgcc gatcgcgcat
     1621 gccagcagga aggctatacc cacgcggtcc cctttggtca gccagtaggc aactgtagcc
     1681 tttttgcggg ttccttatgt ttgaatacgg aattcacttt gtcatatcag tgccatcatt
     1741 ccgccttccc tgtctttctc taaatatttt aatcagcgag gggatcttcg ctgattaaag
     1801 aaatagcggt aatgcgtttt attcccattc gtatttttaa taattgaagt ttatatttta
     1861 ccttttgcaa ataataaaat aacaaattat agtgacgcca cagcaacaaa tataaccttt
     1921 gtggagcact atcatgctga aaccagaaat gattgaaaaa cttaatgagc agatgaacct
     1981 ggaactgtac tcttcactgc tttatcagca aatgagcgcc tggtgcagct atcatacctt
     2041 cgaaggtgct gccgcgttcc tgcgccgtca cgcccaggaa gagatgacgc atatgcagcg
     2101 tctgtttgat tacctgactg ataccggcaa tttaccgcgt attaataccg ttgaatctcc
     2161 gtttgctgaa tattcctcac ttgatgaatt attccaggaa acctataaac acgaacaatt
     2221 aatcacccag aaaattaacg aactggctca tgctgcaatg accaatcagg actacccaac
     2281 atttaatttc ctgcagtggt atgtttctga gcagcatgaa gaagagaaac tgttcaaatc
     2341 gattattgat aaattaagcc tggcaggcaa aagcggcgaa ggtctgtatt ttatcgacaa
     2401 agaactctct accctcgaca cacaaaacta atgctctccg gcggcaggtt tactgccgcc
     2461 gtttcaaatc agtgacggca aatcttactc tgatcggtcg aaaaaccgtc tttcgaaggt
     2521 ataaatttgc cttttgctgc cagaaacgct accagctctc ccgctgtcat cccttctgcc
     2581 gagcaggtgt gaaaacgtgc ctgttcacca aaacgcgctt taatcgcagc ttccagactg
     2641 gcatgcgtat attgctcgcc tgattcaatc atcatattta acacttcatg accgtgaata
     2701 gagtccatcg ttcctcctca aaaaaagcct agcgtagcga ttgccgctta tgaagacttt
     2761 gcgccagcgc aggactgaat gctttttatt gtacatttat atttacacta tatgtaacgt
     2821 cggtttgacg aagcagccgt tatgccttaa cctgcgccgc agatatcact cataaagatc
     2881 gtcaggacag aagaaagcgt gaaaaacaga accctgggaa gtgtttttat cgtggcggga
     2941 accacaattg gcgcaggcat gctggcaatg ccgctggctg cggccggtgt tggttttagc
     3001 gttacgttaa tcttgttgat tgggctttgg gcgttgatgt gctacacggc gctattactg
     3061 ctggaggtgt accagcatgt tccggcagat accggtctgg gcacgctggc aaaacgctat
     3121 ctgggacgct acggtcaatg gctgacgggc ttcagtatga tgttcttaat gtatgctctg
     3181 actgcggcat acatcagcgg tgtcggtgaa ttgttggcct ccagcatcag cgactggaca
     3241 ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca cttttgttgc cggtggcgtg
     3301 gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt ttctgttcag cgccaaaatt
     3361 atttttctgg tggtgatgct ggtattgctg ctaccgcata ttcacaaagt gaatctttta
     3421 accctgccgt tgcaacaggg gctggctctg tcagcaatcc cggtgatttt tacctcattt
     3481 ggttttcacg gtagcgtgcc gagtattgtc agctatatgg atggcaacgt tcgtaagcta
     3541 cgctgggtgt ttataaccgg tagtgcgatc cccctggtgg catatatttt ctggcaggtg
     3601 gcaacgcttg gcagcattga ttcaacaacc tttatgggat tgctggctaa tcatgctgga
     3661 ttaaacgggc tgttacaggc gttacgcgaa atggtggcct ctccgcatgt tgagctggca
     3721 gtgcatttat ttgctgattt agccctcgcc acgtcatttc tcggcgttgc gttaggctta
     3781 tttgattatc tggctgattt atttcagcgt tcaaataccg ttggtggacg gttgcaaact
     3841 ggtgcaatta cctttctgcc gccgttggcg tttgcactgt tttatccacg aggatttgtg
     3901 atggcgctgg gttacgccgg tgtggcgctg gcggtactgg cattgattat cccttcgctg
     3961 ttgacctggc aaagcagaaa gcacaatcct caggcgggtt accgggtcaa aggtggtcgt
     4021 ccggcgctgg tggtggtgtt tctctgtggt attgctgtga ttggcgtgca atttttgatt
     4081 gcggcagggt tgttaccaga agtggggtga tcagatagcc tcaaattcct tattgggtgc
     4141 cagaattaac gctgacaccc aatttggcct cttaatgcag gcagcactgc ttaaatttct
     4201 taccactacc gcacgggcaa ggatcgttac gccccggttt ctcttctgct ttgatcggtt
     4261 gctgaacagc tttttcctgc ggatgcgcca tccagtacgc atgtagatca agcgccgcca
     4321 gtcgaatggc atctacgctc tcttcaaacg cttctggcga catcttttct acccgctcga
     4381 agttttcctc agtaccgtgc agcgcaatcg cctccagcgc tggttttaac gaatcgggca
     4441 acgttgacca gtcagaaagt gccacgcccc gcatatagcc aaagcaccac tcctcaacaa
     4501 tcgtcagctc gctgccatca acttctcgca agccgaataa cggctcaaac tgctccggga
     4561 attcgttcag acgctctgcg gtatcggcca tatgttgaaa agccaggttc ataaagcgcg
     4621 tcatctcttt ctctgacgcc cagcgcggca catagtcagc cccaccccac acggcaacca
     4681 gccactgttc cggttcaatc tcttgcggag aactcaacac cgccgtcaat aaaccgtcca
     4741 gctccgccac atcaaggatg gcgtggttag tgttgtattt ggtcagaata tcgtccagcc
     4801 attccaactc actttcgttt aacggtcccg ttttcatacg cttttccttg tggatctcaa
     4861 ctcgccagca cctatcttac atgccggtcc gtatcagaga tactttttga gtggctttgc
     4921 cggtgattaa aaattaagga gggtgtaacg acaagttgca ggcacaaaaa aaccacccga
     4981 aggtggtttc acgacactgc ttattgcttt gattttattc ttatctttcc catggtaccc
     5041 ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc cttgtgtcta
     5101 ccgattccac catccgggct cgggaagaaa gtggaggcgc gttccggagt cgaaccggac
     5161 tagacggatt tgcaatccgc tacataaccg ctttgttaac gcgccaaatt cttcaggcct
     5221 ttcagccaga catccgcttg acgccgatgt cttttaaact ggagcgggaa acgagactcg
     5281 aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt cccgcattca
     5341 tcaagcaatc agttaatcac ttgattttat tatcgtctgg caatcagtgc cgccgttcga
     5401 tgcgttgcat tctacttacc tggcgcgatg agtcaacgat atttttcacc acttttgatc
     5461 gtttgctgaa aattacgccg aaacgatcac tgatcaagca aatctgcacg cgcagcgctc
     5521 aaatattgca acattgacca cagagtcagt accgcagcca caaagaaaag tgcaataccg
     5581 gcgtactcaa cccaaatgtt cggacgccac agcagccatg ccaacgccac catctgggca
     5641 gtggttttca ctttcccaat ccaggagaca gccacgctac tgcgtttacc caactccgcc
     5701 atccattcgc gtagcgcaga aataataatt tcacgggcga tcatcgttgc tgccggtaag
     5761 gtcacccacc agctgtggta atgctcggtt accagcacca tggcgatagc cacgagaact
     5821 ttatctgcta cagggtcaag gaaagcacca aaccgggtac tctggttcca gcggcgtgcc
     5881 agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga aaatgagcgc ggcggcaaac
     5941 ggcgaccagg tgacaggcag ataaaagacc aatacaaaga atgggataag gatgacacgg
     6001 aacagtgtaa gcaacgtagg gatattaaat tgcataatga cgggtaacta tctgttgtca
     6061 gtaagattac ccctatgttg ctacagagac atcaatgttt caacgaccag aagatctttt
     6121 ctgccagacc ttgcgaaata cccggcactt ttgcaatttc ctcgacgctg gcgttacgta
     6181 aaccttgcaa accgcccata tatttcaaca acatttgccg acgttttggc ccgacgcctt
     6241 caatggtttc cagggaactg gtatttttga ccttcgcccg ttttttacgg tgcccgccaa
     6301 tcgcgtgatc atgtgattca tcgcgaatat gctggataac atgcagcgcg ggagaatctg
     6361 gcggcaaact gaatccctca ccttccggct caaagaacag cgtttccagc ccagccttac
     6421 gatctgctcc tttggcaacg ccaagtagca gcggatgatt tttatcccat gagacatcca
     6481 gttcggcgaa gacatttttc gcctgcgcaa gctggccttt gccgccgtcg ataaggatca
     6541 catccgggat cttactgtcg tcaatggctt taccataacg ccgacgcagc acctgattca
     6601 tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt atagcgccga tactccgcac
     6661 gcagcgggcc gttagcatca aacaccacac aggaagcgac ggtttgttcg cccatggtat
     6721 ggctgatgtc aaagcactcc atccgcttca cttccggcaa tttcaacacg ctggcaagcg
     6781 cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt gctggttaag gccgtcgccg
     6841 cattggtgcg cgcgagtttc agataacgcg ccctatcgcc gcgaggtttg gtttgaacat
     6901 taatcttgcg tcccgccagt tctgaaaggg aatcggcgag cagcgtttta tcgctaagat
     6961 taaaatcgag caggatctca cccggtaagg tgcgcatctg gctgccttgt aaatagaact
     7021 ggcctacgaa ggtttctacc acctcgctca gttccgtacc gccaggcact ttcgggaaat
     7081 agctgcggct gccgagcact ttgccctgac gaatgaacaa tacgtggaca caagccatgc
     7141 ccgcatcgaa cgccacacca ataacgtcga ggtcgtcgcc ggtattggaa acgaattgtt
     7201 tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat acgtgcagct tcttcaaact
     7261 ccagattctg gctggcagtt tccatacgac taatgagttg cgtaagcacc tgatcatctt
     7321 tgccagacaa aaacaggcgc acatactcaa cctgctgagc gtattcttct tcactcacca
     7381 gtccttcaac gcacggtccc agacagcgcc ctatctgata ttgcagacac ggacgcgagc
     7441 gattgcgata aacactattt tcgcactggc gaatggggaa aatcttttgc agtagcgcca
     7501 gtgtttcacg tacggcatag ccattcggga acgggccgaa atattcacct ttggcatgct
     7561 tcgcaccacg atgcatcgcc agacgcgggt gggtatcgcc actcaggaag ataaaaggat
     7621 aagatttatc atcgcgtagc aaaacgttgt aacgcggctg atagagtttg atgtagttgt
     7681 gttccagcaa cagcgcttcg gtttctgtat gcgtaaccgt tacatcaatt tgctggatct
     7741 gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct gcggaaatag ctggaaagcc
     7801 gttttttcag gtctttcgct ttgccgacat agataaccgt accaccagca tcgtacatgc
     7861 gataaacgcc tggctggcag gttacggttt ttaaaaacgc ttttgcgtca aactgatcac
     7921 tcactgactt gataatgtct ccgcattaca cagaccatgg cgaattgcca ggtgagtcag
     7981 ctcaacatcg ccatgaatgt ttagtttact gaacatacga tagcggtagc tgttcaccgt
     8041 tttcggactg agattgagct gttctgagat ctcattgacc ttctggccct tggtgatcat
     8101 cagcataatc tgcaattcac gttcagacaa actggcaaat gggctttctg ttttttctgg
     8161 ttcgatctgg cttaacgcca tttgttgagc gatatcagaa gcaatgtaac gctgccctga
     8221 atagacagaa cgaatcgcac tcacgacttc ctgcggagcc gcgcctttgc tgaggtagcc
     8281 cgcagcaccg gcctgcatga ctttcgctgg taaagggttt tctgtatgga cggtaagcat
     8341 gatgattttg acatcagctg tggaacgcgc gattttacgc gtcgcctcaa gaccgccaat
     8401 gcccggcata ctcatgtcca ttagcaccac gtcaacggca tttgtccggc accacttaac
     8461 ggcgtcttca ccgcacgatg cctcaccgac gacttttata ccctttatat cttccagaat
     8521 gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca agtagaacgt tgatcaaagg
     8581 aatatctcca gaaataggga taacgctact gatagttagt cattcgtata ttagcggttt
     8641 ttattgcaac tttgaaacgt taaaaatggt gggtattcga tttttctctc gtttttgtaa
     8701 attcgattgt ccacagttat ggtcacgccc gtcaccaacg gcttccggga tcgtttcaca
     8761 gtaagcccgg cgatgaaatg tgacaaaaat tgactttatt cagcaaaaat gaaaatcagc
     8821 ccgctgaata tgtaacatta attaacccga acaatacggg caaaaaacat tcatgataaa
     8881 a
//
LOCUS       DAZOYL010000161         8740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZOYL010000161 DAZOYL010000000
VERSION     DAZOYL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.161"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            111..2645
                     /gene="mrcB"
                     /locus_tag="ACHUOY_002710"
     CDS             111..2645
                     /gene="mrcB"
                     /locus_tag="ACHUOY_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR0683450.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2865..5054
                     /gene="fhuA"
                     /locus_tag="ACHUOY_002711"
     CDS             2865..5054
                     /gene="fhuA"
                     /locus_tag="ACHUOY_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR0683451.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            5105..5902
                     /gene="fhuC"
                     /locus_tag="ACHUOY_002712"
     CDS             5105..5902
                     /gene="fhuC"
                     /locus_tag="ACHUOY_002712"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR0683452.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5902..6792
                     /gene="fhuD"
                     /locus_tag="ACHUOY_002713"
     CDS             5902..6792
                     /gene="fhuD"
                     /locus_tag="ACHUOY_002713"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR0683453.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6789..>8740
                     /gene="fhuB"
                     /locus_tag="ACHUOY_002714"
     CDS             6789..>8740
                     /gene="fhuB"
                     /locus_tag="ACHUOY_002714"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR0683454.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPY"
BASE COUNT         1918 a         2144 c         2531 g         2147 t
ORIGIN      
        1 caagggagag gggaccgatc gagcacaaat tttgagagat atcttcttct gtcttgtaac
       61 agaagaacag aaaatcgggc ttttgcgcct gaatattgcg gagaaaaagc atggccggga
      121 atgaccgcga gccaattgga cgcaaaggga aaccgacgcg tccggtcaaa caaaaggtaa
      181 gccgtcgtcg ttacgaagat gacgatgatt acgacgatta tgatgactat gaggatgaag
      241 aaccgatgcc gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt ggcaaacgcg
      301 gctggctatg gctgctgtta aaactggcta tcgtttttgc cgtgctgatc gccatttacg
      361 gcgtttatct cgatcaaaaa attcgtagcc gtattgatgg caaggtctgg caactgcccg
      421 cggcagttta tggccgaatg gtcaaccttg aaccagatat gaccatcagc aagaacgaaa
      481 tggtgaagct gctggaggca acacagtatc gtcaggtgtc gaaaatgacc cgtcctggcg
      541 aatttaccgt gcaggccaac agcattgaga tgatccgccg tccgtttgat ttcccggaca
      601 gcaaagaagg gcaggtgcgt gcgcgtctga cctttgatgg cgatcatctg gcgacgatcg
      661 tcaatatgga gaacaaccgt cagttcggtt tcttccgtct tgatccgcgc ctgatcacca
      721 tgatctcttc gccaaacggt gagcagcgtc tgtttgtgcc gcgcagtggt ttcccggact
      781 tgctggtgga tactttgctg gcgacagaag accgtcattt ctacgagcat gatgggatca
      841 gtctctactc catcggacgt gcggtgctgg caaacctgac cgccggacgc acggtgcagg
      901 gagcgagtac gctgacgcaa cagctggtga aaaacctgtt cctctccagc gagcgttctt
      961 actggcgtaa agcgaacgaa gcttacatgg cgctgatcat ggacgcgcgt tacagcaaag
     1021 accgtattct tgagctgtat atgaacgagg tgtatctcgg tcagagcggc gacaacgaaa
     1081 tccgcggctt cccgcttgcg agcttgtatt actttggtcg cccggtagaa gagctcagcc
     1141 tcgaccagca ggcgctgtta gtcggtatgg tgaaaggggc gtccatctac aacccgtggc
     1201 gtaacccgaa actggcgctg gagcgacgta atctggtgct gcgtttgcta caacaacagc
     1261 agattatcga tggtgaactg tatgaaatgc tcagcgcgcg tcctctgggc gtacagccgc
     1321 gcggtggggt aatctctcca cagccagcct ttatgcaact ggtgcgtcag gagctgcagg
     1381 cgaaattagg cgataaggta aaagatctct ccggcgtgaa gatcttcact acctttgact
     1441 cggtggccca ggacgcggca gaaaaagccg ccgtggaagg cattccggca ctgaagaaac
     1501 agcgtaagtt gagcgatctt gaaactgcaa ttgtggtcgt cgaccgcttt agtggtgaag
     1561 ttcgtgcgat ggtgggaggt tctgagccgc agtttgcggg ctacaaccgt gcgatgcagg
     1621 cgcgtcgttc gattggttcc cttgcaaaac cagcgactta tctgacggct ttaagccagc
     1681 cgaaaatcta tcgcctgaac acgtggattg ccgatgcgcc aatcgcgctg cgtcagccga
     1741 atggccaggt ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc ggcagagtga
     1801 tgctggtgga tgcgttgacc cgttcgatga acgtgccgac ggtaaatctg gggatggcgc
     1861 tggggctgcc tgcagttacg gagacctgga tcaaactggg cgtgccgaaa gatcagctgc
     1921 atccggttcc ggcgatgctg ctgggggcgt tgaacttaac gccaatcgaa gtggcgcagg
     1981 cattccagac catcgccagc ggcggtaacc gtgcaccgct ttctgcactg cgttcggtaa
     2041 tcgcggaaga tggcaaggtg ctgtatcaga gcttcccgca ggcagaacgc gctgttccgg
     2101 cgcaggcggc gtatctgaca ctatggacca tgcagcaggt ggtacaacgt ggtacgggtc
     2161 gtcaacttgg ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg actaccaaca
     2221 ataacgtaga tacctggttt gcgggcattg acggcagcac ggtgaccatc acctgggtag
     2281 gccgtgataa caaccagccg accaaactgt atggtgccag cggggcaatg tctatttatc
     2341 agcgttatct ggctaaccag acgccaacgc cgctgaatct tgttccgcca gaagatattg
     2401 cagatatggg cgtggactac gacggcaact ttgtttgcag cggtggcatg cgtgtcttgc
     2461 cggtctggac cagcgatccg caatcgctgt gccagcagag cgagatgcag cagcagccgt
     2521 caggcaatcc gtttgatcag tcttctcagc cgcagcaaca gccgcaacag caacctgctc
     2581 agcaagagca gaaagacagc gacggtgtag ccggttggat caaggatatg tttggtagta
     2641 attaacatct aagcgtgaaa taccggatgg cgaattgcca tccggtaaaa taacatccca
     2701 cctaaagtat taaccctttc ttttcatctg gttgtttatt aacccttcag gaacgctcag
     2761 attgcgtacc gcttgcgaac ccgccagcgt ttcgaatatt atcttatctt tataataatc
     2821 attctcgttt acgttatcat tcactttaca tcagagatat accaatggcg cgttccaaaa
     2881 ctgctcagcc aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca gcggttagcg
     2941 gcatgtctgt ttatgcacag gcagcggttg aaccgaaaga agacactatc accgttaccg
     3001 ctgcacctgc gccgcaagaa agcgcatggg ggcctgctgc aactattgcg gcgaagcact
     3061 ctgctactgc gactaaaacg gatacaccaa ttgaaaaaac gccgcagtct atttcggttg
     3121 tgacaaatga agagatgcag atgcgtcaat ttcagtctgt aaaagaagca ttaggttata
     3181 cacctggcgt tacggttagc agtcgtggtg cttctaatac atatgatttt gttatcattc
     3241 gtggtttctc atctgttggt ctgaatcaaa ataattacct tgatggacta aaacttcagg
     3301 gcaactttta taacgatgct gtgattgatc cttacatgct cgagagggtt gaactgatgc
     3361 gtgggccgac gtctgttctc tatggaaaaa gtaatcctgg tgggattatt tctatggtga
     3421 gtaagcgtcc gaccactgaa cccctgaaag aaattcagtt taaaatgggg acggataatc
     3481 tgtttcagac cggattcgat ttcagtgatg cactggatga taacggtgag ttctcttacc
     3541 gtttgacggg cctcgctcgc tcaacaaacg aacagcagaa aaactctgaa tctcagcgtt
     3601 ataccattgc tccatcattc tcatggcgtc cagacgacaa aaccaatttt actttcctgt
     3661 cctatttcca gaatgaacca gaaacgggtt attacggttg gttgccgaaa gaggggaccg
     3721 ttgagccatt gcctaatggt aagcgtctac cgactgactt caatgaaggt gcatcgaata
     3781 atacatactc ccgtaaccag aaaatggtgg gatatagttt tgaacatggt tttaatgaca
     3841 ccttcaccgt gcgtcagaat ctgcgtttca gtgaaatgaa aacctcacag aaaagtgttt
     3901 atggcacagg gattgctggt gatggtcata ccctaaaccg cgggacagtg gtggataatg
     3961 agcgtctaca aaactttagc gttgataccc aacttgaaag taagtttgct acaggtgacg
     4021 tcgagcatac tttgctgaca ggggtagact tcatgcggat gcgtaatgat atcaatgcca
     4081 gctttggatc cgcaccatcc atcgatcttt ataacaaata tcaccctgaa tactttgcat
     4141 ttggtagcgc agagccatac cagatgaatg aaagcaaaca aacaggtatt tatgttcagg
     4201 atcaggcgga atggaataaa tgggtattca ctctgggggg acgctacgat tggtctaagc
     4261 aagcgaccac tgttcgtgaa aactcttata cgccgactga aggttatatt gagcgcaatg
     4321 atcatcagtt cacctggcgc ggtggtgtaa attacttatt cgataatggt atttcacctt
     4381 actttagcta tagccagtcc tttgaaccga gtgctttcga tctgtggagc aacccgcgca
     4441 tttcctataa gccatcgaaa ggtgaacagt atgaagctgg cgtaaaatat gttccgaatg
     4501 atatgccggt cgttgttacg ggcgcagtct atcaattgac gaaaacaaat aacctgacag
     4561 cagacccaac aaacccatta gcgcaagtcc cagcaggtga gattcgcgct cgtggtgtgg
     4621 aacttgaagc aaaagcggcg ttaaatgcca atattaactt gacggcttct tatacctaca
     4681 ccgatgcgga atacaccaaa gacaccaatc tcaaaggtaa aactccagaa caagtaccgg
     4741 agcatatggc atctctctgg ggggattata ccttcaatga agggccgctt tctggtttaa
     4801 cattgggaac aggtggtcgt tttattggtt ccagctatgg tgatccggca aacactttta
     4861 aagtgggtag cacagctgta atggatgctg ttgtaaaata tgatctggca cgctttggta
     4921 tggcgggatc cagccttgct gtgaacgtca acaatttgct cgatcgtgag tatgttgcca
     4981 gttgcttcca gacttatggc tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa
     5041 ccttccgttt ctaatttctc ttttgggaca cggatttccg tgcccatttc acaagttggc
     5101 tgttatgcag gaatacacga atcattccga taccactttt gcactgcgta atatctcctt
     5161 tcgtgtgccc gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg ccgggaaagt
     5221 gaccggtctg attggtcaca acggttctgg taaatccact ctgctcaaaa tgcttggtcg
     5281 tcatcagcct ccgtcagaag gggagattct tcttgatgcc cagccgctgg aaagctggag
     5341 cagcaaagcg tttgcccgca aagtggctta tttgccgcag cagcttcctc cggcagaagg
     5401 gatgaccgtg cgtgaactgg tggcgattgg tcgttacccg tggcatggcg cgctggggcg
     5461 ctttggtgcg gcagatcgcg aaaaggtcga ggaagctatc tcgctggttg gcttaaaacc
     5521 gctggcgcat cggctggtcg atagtctctc tggcggcgaa cgtcagcggg cgtggatcgc
     5581 catgctggtg gcgcaggata gccgttgtct gttgctcgac gaaccgactt cggcgctgga
     5641 tatcgcccac caggttgatg tgctggcgct ggtgcaccgt ttaagtcagg aacgtggcct
     5701 gacggtcatt gccgtgttgc acgatatcaa tatggcggca cgctactgtg attatctggt
     5761 cgccctgcgc agtggtgaaa tgattgctca gggaacgcct gcggaaatta tgcgcggcga
     5821 aaccctcgaa atgatttatg gcatcccgat gggtattttg ccgcatccgg cgggtgctgc
     5881 acctgtgagt tttgtttatt gatgagcggc ttacctctta tttcgcgccg tcgactgtta
     5941 acggcgatgg cgctttctcc gttgttatgg cagatgaata ccgcccacgc ggcggctatt
     6001 gaccccaatc gtattgtggc gctggagtgg ttgccggtgg aattactgct ggcgctcggc
     6061 atcgtgcctt acggcgtggc ggataccatc aactatcgcc tgtgggtcag cgaaccacca
     6121 ttgccggact cagtgattga cgtcggtttg cgtacagaac ctaaccttga actgctgacc
     6181 gaaatgaaac cgtcgtttat ggtctggtcg gcaggatatg gcccttcatc agaaatgctg
     6241 gcacgtattg cgccgggtcg cggatttaac ttcagtgacg gcaaacagcc gttggcgatg
     6301 gcgcgtaaat cgctgacgga aatggcagat ttacttaacc tgcaaagcgc agcggaaacg
     6361 catttagcgc aatacgaaga ctttatccgc agcatgaaac cccgctttgt gaagcgtggt
     6421 gcacgcccgt tactgctgac gacgcttatc gatccgcgcc atatgctggt cttcggtcca
     6481 aacagcttgt tccaggaaat tctcgatgag tacggcatcc caaatgcctg gcaaggggaa
     6541 accaacttct ggggcagtac cgccgtcagt atcgatcgtc tggcagcgta taaagacgtt
     6601 gatgtgctct gttttgatca cgacaacagc aaagacatgg atgcgctaat ggcaaccccg
     6661 ctgtggcagg ccatgccgtt tgtccgcgcc ggacgctttc agcgcgtacc tgcagtctgg
     6721 ttttatggtg cgacactctc ggcaatgcac tttgtgcgcg ttctggataa cgccatcgga
     6781 ggtaaagcgt gagtaaacga attgcgcttt ttccggcgtt attgctggcg ctgttagtga
     6841 ttgtcgctac ggcgctcacc tggatgaact tctcgcaggc gctgccgcgt agccagtggg
     6901 cgcaggctgc ctggtcgcca gacatcgatg tcatcgagca gatgattttt cactacagct
     6961 tgttgccgcg tctggcgatt tcgctgctgg tgggcgcggg cctggggctg gtgggcgtgc
     7021 tgtttcagca agtgctgcgt aacccgctgg cggagccgac gacgcttggc gttgctacag
     7081 gcgcgcaact gggaattacc gtcaccacac tttgggcgat cccaggtgct atggcgagcc
     7141 agtttgccgc actggtgggc gcttgtgtcg ttggcttaat cgtctttggc gtcgcgtggg
     7201 ggaaacggct ttcgccggtt acgcttattc tcgcggggct ggtggtgagc ctttattgcg
     7261 gggcaattaa tcagctactg gttatcttcc atcacgacca gctgcaaagc atgttcctgt
     7321 ggagcaccgg aacgctgacg caaaccgact ggggcggcgt tgagcgttta tggccgcagt
     7381 tgctgggcgg cgtgatgttg acgttattgc tacttcgccc gttaaccctg atggggcttg
     7441 atgatggcgt ggcgcgcaat ctcgggctgg ccttgtcgct cgcgcgtctg gcggcgctgt
     7501 cgctggcgat agtcatcagt gcgctgctgg tgaatgcggt ggggattatc ggctttatcg
     7561 gtttgttcgc gccgctgctg gcgaaaatgc tgggggcgcg gcgtttgttg ccacggttga
     7621 tgctggcgtc gctgattggg gcgctgatcc tctggctttc cgatcaaatc atcctctggc
     7681 tgactcgcgt gtggatggaa gtgtccaccg gttcggtcac tgcgttgatc ggtgcgccgc
     7741 tgctactgtg gctgttgccg cgtttacgca gcattagcgc gccggatatg aaggtcaacg
     7801 atcgtgtcgc ggctgaacgc caacatgtgc tggcgtttgc cctcgcgggc ggcgtgctgc
     7861 tgttgatggc tgtggtggtg gcgctgtcgt ttggtcgtga tgcgcacggc tggacgtggg
     7921 cgagcggggc gttgctcgag gatttgatgc cctggcgctg gccgcgaatt atggcggcgc
     7981 tgtttgcggg cgtcatgctg gcggtggcgg gctgtattat tcagcggctg accggaaacc
     8041 cgatggcaag cccggaagtg ctggggatta gctctggcgc ggcgtttggc gtggtgttaa
     8101 tgctgtttct ggtgccgggt aatgcctttg gctggctgtt acccgcgggc agtatcgggg
     8161 cggcggtgac gctgttgatc attatgatcg ccgccggccg cggtggattt tccccacacc
     8221 gtatgttact ggcggggatg gcgttaagta ccgcattcac catgcttttg atgatgttgc
     8281 aggcgagcgg tgacccgcga atggcgcaag tgctgacctg gatttccggt tcgacctaca
     8341 acgcgaccga tgcgcaggtc tggcgtaccg gaattgtgat ggtgattttg ctggcgatta
     8401 ccccgctgta ccgccgctgg ctgaccattt taccgctggg tggtgatacc gcacgagccg
     8461 taggaatggc gctgacgccg acgcgaattg cgctgctgct gttagcggct tgcctgacgg
     8521 cgaccgcgac gatgactatt ggaccgttga gttttgttgg tttaatggca ccgcatattg
     8581 cgcggatgat gggctttcga cggacgatgc cacacatcgt aatttcggcg ctggtgggtg
     8641 gtttactgct ggtattcgct gactggtgtg ggcggatggt gctgtttcca ttccagatcc
     8701 cggcggggct gctgtcgacc tttatcggcg cgccatattt
//
LOCUS       DAZOYL010000162         8724 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZOYL010000162 DAZOYL010000000
VERSION     DAZOYL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.162"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(279..542)
                     /gene="rpsT"
                     /locus_tag="ACHUOY_002715"
     CDS             complement(279..542)
                     /gene="rpsT"
                     /locus_tag="ACHUOY_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR0683455.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            645..863
                     /locus_tag="ACHUOY_002716"
     CDS             645..863
                     /locus_tag="ACHUOY_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR0683456.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            871..1812
                     /gene="ribF"
                     /locus_tag="ACHUOY_002717"
     CDS             871..1812
                     /gene="ribF"
                     /locus_tag="ACHUOY_002717"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR0683457.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQKEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1855..4671
                     /gene="ileS"
                     /locus_tag="ACHUOY_002718"
     CDS             1855..4671
                     /gene="ileS"
                     /locus_tag="ACHUOY_002718"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR0683458.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4671..5165
                     /gene="lspA"
                     /locus_tag="ACHUOY_002719"
     CDS             4671..5165
                     /gene="lspA"
                     /locus_tag="ACHUOY_002719"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR0683459.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5290..5739
                     /gene="fkpB"
                     /locus_tag="ACHUOY_002720"
     CDS             5290..5739
                     /gene="fkpB"
                     /locus_tag="ACHUOY_002720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0683460.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5741..6691
                     /gene="ispH"
                     /locus_tag="ACHUOY_002721"
                     /gene_synonym="lytB"
     CDS             5741..6691
                     /gene="ispH"
                     /locus_tag="ACHUOY_002721"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR0683461.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6757..7671
                     /gene="rihC"
                     /locus_tag="ACHUOY_002722"
     CDS             6757..7671
                     /gene="rihC"
                     /locus_tag="ACHUOY_002722"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR0683462.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7838..8659
                     /gene="dapB"
                     /locus_tag="ACHUOY_002723"
     CDS             7838..8659
                     /gene="dapB"
                     /locus_tag="ACHUOY_002723"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR0683463.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         2000 a         2169 c         2467 g         2088 t
ORIGIN      
        1 tcttaagttt aatacctcaa tgtgtatcac aatatccaca ttctttgtag gggagtctgg
       61 agattgagta gatattcttg ttcagaatgt atcagccgat ggttctacga ttcttaagcc
      121 acgaagagtt cagaaagtgt aacggcatgt ctgcaaaagc cagcatcttt tgactatctg
      181 gcaaccggta gtgtgttctc tcacgcatca taaaagcagc aggcataaaa aaaacccgct
      241 tgcgcgggct ttttcacaaa gcttcagcaa attggcgatt aagccagttt gttgatctgt
      301 gcagtcaggt tagccttatg acgtgcagct ttgtttttgt ggatcagacc tttagcagcc
      361 tgacggtcca cgatcggttg catttcgtta aatgctttct gtgcagcagc tttgtcgcca
      421 gcttcgatag ctgcgtatac tttcttgatg aaagtacgca tcatagagcg acggcttgcg
      481 ttgtgcttac gagccttttc agactgaatg gcgcgcttct tagctgattt gatattagcc
      541 aaggtccaac tcccaaatgt gttctatatg gacaattcaa aggccgagga atatgccctt
      601 ttagccttct tttgtcaatg gatttgtgca aataagcgcc gttaatgtgc cggcactcgt
      661 tacgtagtga tggcgcagga ttctaccagc ttgcggggtg tgaatacagc ttttccgcga
      721 taaaaattgc agcaggcggt cactttcttc ccgtgatttg cgccatggca atgaaaagcc
      781 acttctttct gatttcggta ctcaatcgcc ggttaacctt gaccgctgta caaggtatac
      841 tcggacgatt ttcactgttt tgagccagac atgaagctga tacgcggcat acataatctc
      901 agccaggccc cgcaagaagg gtgtgtgctg actattggta atttcgacgg cgtgcatcgc
      961 ggtcatcgcg cgctgttaca gggcttgcag aaagaagggc gcaagcgcaa cttaccggtg
     1021 atggtgatgc tttttgaacc tcaaccactg gaactgtttg ctaccgataa agccccggca
     1081 agactgaccc ggctgcggga aaaactgcgt taccttgcag agtgtggcgt tgattacgtg
     1141 ctgtgcgtgc gtttcgacag gcgtttcgcg gcgttaaccg cgcaaaattt catcagcgat
     1201 cttctggtga agcatttgcg cgtaaaattt cttgccgtag gggatgattt ccgctttggc
     1261 gctggtcgtg aaggcgattt cttgttatta cagaaagcta gcatggaata cggcttcgat
     1321 atcactagta cgcaaacttt ttgcgaaggt ggcgtgcgta ttagcagcac cgctgtgcgt
     1381 caggcgctgg cggatgacaa tctggctctg gcagaaagtt tactggggca cccgtttgcc
     1441 atctccgggc gtgtagtcca cggtgatgaa ttagggcgca ctataggttt cccgacggcg
     1501 aatgtaccgc tacgccgtca ggtttccccg gtgaaagggg tttatgcggt agaagtgctg
     1561 ggcctcggtg aaaagccgtt acccggcgtg gcaaacatcg gaacacgccc aacggttgcc
     1621 ggtattcgcc agcagctgga agtgcatttg ttagatgttg caatggacct ttacggtcgc
     1681 catatacaag tagtgctgcg taaaaaaata cgcaatgagc agcgatttgc gtcgctggac
     1741 gaactgaaag cgcagattgc gcgtgatgaa ttaaccgccc gcgaattttt tgggctaaca
     1801 aaaccggctt aagcctgtta tgtaatcaaa ccgaaacacg gaaccgagaa tctgatgagt
     1861 gactataaat caaccctgaa tttgccggaa acagggttcc cgatgcgtgg cgatctcgcc
     1921 aagcgcgaac cgggaatgct ggcgcgttgg actgatgatg atctgtacgg catcatccgt
     1981 gcggctaaaa aaggcaaaaa aaccttcatt ctgcatgatg gccctcctta tgcgaatggc
     2041 agcattcata ttggtcactc ggttaacaag attctgaaag acattatcgt gaagtccaaa
     2101 gggctttccg gttatgactc gccgtatgtg cctggctggg actgccacgg tctgccgatc
     2161 gagctgaaag tagagcaaga atacggtaag ccgggtgaga aattcaccgc cgccgagttc
     2221 cgcgccaagt gccgcgaata cgcggcgacc caggttgacg gtcaacgcaa agactttatc
     2281 cgtctgggcg tgctgggcga ctggtcgcac ccgtacctga ccatggactt caaaactgaa
     2341 gccaacatca tccgcgcgct gggcaaaatc atcggcaacg gtcacctgca caaaggcgcg
     2401 aagccagttc actggtgcgt tgactgccgt tctgcgctgg cggaagcgga agttgagtat
     2461 tacgacaaaa cttctccgtc catcgacgtt gcttttcagg cggtcgatca ggatgcactg
     2521 aaagcaaaat ttgccgtaag caacgttaac ggcccaatct cgctggtgat ctggaccact
     2581 acgccgtgga ctctgcctgc gaaccgcgca atctctattg cacctgattt cgactatgcg
     2641 ctggtgcaga tcgacggtca ggccgtgatt ctggcgaaag atctggttga aagcgtaatg
     2701 cagcgtatcg gcgtgaccga ttacaccatt ctcggcacgg taaaaggtgc ggagcttgag
     2761 ttgctgcgct ttacccatcc gtttatgggc ttcgacgttc cggcaatcct cggcgatcac
     2821 gttaccctag atgccggtac cggtgccgtt cacaccgcgc ctggccacgg cccggacgac
     2881 tatgtgatcg gtcagaaata cggcctggaa accgctaacc cggttggccc ggacggcact
     2941 tatctgccgg gcacttatcc gacgctggat ggcgtgaacg tcttcaaagc gaacgacatc
     3001 gtcgttgcgc tgctgcagga aaaaggcgcg ctgctgcacg ttgagaaaat gcagcacagc
     3061 tatccgtgct gctggcgtca caaaacgccg atcatcttcc gcgcgacgcc gcagtggttc
     3121 gtcagcatgg atcagaaagg tctgcgtgcg cagtcactga aagagatcaa aggcgtgcag
     3181 tggatcccgg actggggcca ggcgcgtatc gagtcgatgg ttgctaaccg tcctgactgg
     3241 tgtatctccc gtcagcgcac ctggggcgta ccgatgtcac tgttcgtgca caaagacacg
     3301 gaagagctgc atccgcgtac tctcgaacta atggaagaag tggcaaaacg cgttgaagtt
     3361 gacggcatcc aggcgtggtg ggatcttgat gcgaaagaga tcctcggcga tgaagctgat
     3421 cagtacgtga aagtgccgga cacattggat gtatggtttg actccggatc tacccactct
     3481 tctgttgttg acgtgcgtcc ggaatttgcc ggtcacgcag cggacatgta tctggaaggt
     3541 tctgaccagc accgtggttg gttcatgtct tccctaatga tctccaccgc gatgaaaggc
     3601 aaagcgccgt atcgtcaggt actgacccac ggctttaccg tggatggtca gggtcgcaag
     3661 atgtctaaat ccatcggcaa taccgtttcg ccgcaggatg tgatgaacaa actgggcgcg
     3721 gatattctgc gtctgtgggt ggcatcaacc gactacaccg gtgaaatggc cgtttctgac
     3781 gagatcctga aacgtgctgc cgatagctat cgtcgtatcc gtaacaccgc gcgcttcctg
     3841 ctggcaaacc tgaacggttt tgatccagca aaagatatgg tgaaaccgga agagatggtg
     3901 gtactggatc gctgggccgt aggttgtgcg aaagcggcac aggaagacat cctcaaggcg
     3961 tacgaagcat acgatttcca cgaagtggta cagcgtctga tgcgcttctg ctccgttgag
     4021 atgggttcct tctacctcga catcatcaaa gaccgtcagt acaccgccaa agcggacagt
     4081 gtggcgcgtc gtagctgcca gactgcgctg tatcacatcg cagaagcgct ggtgcgctgg
     4141 atggcaccaa tcctctcctt caccgctgat gaagtgtggg gctacctgcc gggcgaacgt
     4201 gaaaaatacg tcttcaccgg tgagtggtac gaaggcctgt ttggcctggc agacagtgaa
     4261 gcgatgaacg atgcgttctg ggacgagctg ttgaaagtgc gtggcgaagt gaacaaagtc
     4321 attgagcaag cgcgtgccga caagaaagtg ggtggctcgc tggaagcggc agtaaccttg
     4381 tatgcagaac cggaactgtc ggcgaaactg accgcgctgg gcgatgaatt acgatttgtc
     4441 ctgttgacct ccggcgctac cgttgcagac tataacgacg cacctgctga tgctcagcag
     4501 agcgaagtac tcaaagggct gaaagtcgcg ttgagtaaag ccgaaggtga gaagtgccca
     4561 cgctgctggc actacaccca ggatgtcggc aaggtggcgg aacacgcaga aatctgcggc
     4621 cgctgtgtca gcaacgtcgc cggtgacggt gaaaaacgta agtttgcctg atgagtcaat
     4681 cgatctgttc aacagggcta cgctggctgt ggctggtggt agtcgtgctg attatcgatc
     4741 tgggcagcaa atacctgatc ctccagaact ttgctctggg ggatacggtc ccgctgttcc
     4801 cgtcgcttaa tctgcattat gcgcgtaact atggcgcggc gtttagtttc cttgccgata
     4861 gcggcggctg gcagcgttgg ttctttgccg gtattgcgat tggtattagc gtgatcctgg
     4921 cagtgatgat gtatcgctcg aaggccacgc agaagctaaa caatatcgct tacgcgctga
     4981 ttattggcgg cgcgctgggc aacctgttcg accgcctgtg gcacggcttc gttgtcgata
     5041 tgatcgactt ctacgtcggc gactggcact tcgccacctt caaccttgcc gatactgcca
     5101 tctgtgtcgg tgcggcactg attgtgctgg aaggtttttt gccttctaga gcgaaaaaac
     5161 aataataaac cctgccggat gcgatgctga cgcatcttat ccggcctaca gattgctgcg
     5221 aaatcgtagg ccggataagg cgtttacgcc gcatccggca aaaatcctca aatataagag
     5281 caaacctgca tgtctgaatc tgtacagagc aatagcgccg tcctggtgca cttcacgcta
     5341 aaactcgacg atggcaccac cgccgagtct acccgcaaca acggtaaacc ggcgctgttc
     5401 cgcctgggtg atgcttctct ttctgaaggg ctggagcaac acctgttggg gctgaaagtg
     5461 ggcgataaaa ccaccttctc gttggagcca gatgcggcgt ttggcgtgcc gtcaccggac
     5521 ctgattcagt acttttcccg ccgtgaattt atggatgcag gcgagccaga aattggcgca
     5581 atcatgcttt ttaccgcaat ggatggcagt gagatgcctg gcgtgatccg cgaaattaac
     5641 ggcgactcca ttaccgttga tttcaaccat ccgctggccg ggcagaccgt tcattttgat
     5701 attgaagtgc tggaaatcga tccggcattg gaggcgtaac atgcagatcc tgttggccaa
     5761 cccgcgtggt ttttgtgccg gggtagaccg cgctatcagc attgttgaaa acgcgctggc
     5821 catttacggc gcaccgatat atgtccgtca cgaagtggta cataaccgct atgtggtcga
     5881 tagcttgcgt gagcgtgggg ctatctttat tgagcagatt agcgaagtac cggacggcgc
     5941 gatcctgatt ttctccgcac acggtgtttc tcaggcggta cgtaacgaag caaaaagccg
     6001 cgatttgacg gtgtttgatg ccacctgtcc gctggtgacc aaagtgcata tggaagtcgc
     6061 ccgcgccagc cgtcgtggcg aagagtcgat tctcatcggc cacgccgggc atccggaagt
     6121 ggaaggtaca atgggccagt acagtaaccc ggaaggggga atgtatctgg tcgaatcgcc
     6181 ggacgatgtg tggaaactga cggtcaaaaa cgaagagaag ctctccttta tgacccagac
     6241 cacgctgtcg gtggatgaca cgtctgatgt gatcgacgcg ctgcgtaaac gcttcccgaa
     6301 aattgtcggt ccgcgcaaag atgacatctg ctacgccacg actaaccgtc aggaagcggt
     6361 acgcgccctg gcagaacagg cggaagttgt gttggtggtc ggttcgaaaa actcctccaa
     6421 ctccaaccgt ctggcggagc tggcccagcg tatgggcaaa cgcgcgtttt tgattgacga
     6481 tgcgaaagat atccaggaag agtgggtgaa agaggttaaa tgcgtcggcg tgactgcggg
     6541 cgcatcggct ccggatattc tggtgcaaaa tgtggtggca cgtttgcagc agctgggcgg
     6601 tggtgaagcc attccgctgg aaggccgtga agaaaacatt gttttcgaag tgccgaaaga
     6661 gctgcgtgtc gatattcgtg aagtcgatta agtcattagc agcctaagtt atgcgaaaat
     6721 gccggtcttg ttaccggcat tttttatgga gaaaacatgc gtttacctat cttcctcgat
     6781 actgaccccg gcattgacga tgccgtcgcc attgccgccg cgatttttgc ccccgaactc
     6841 gacctgcaac tgatgaccac cgtcgcgggt aatgtctcgg ttgagaaaac cacccgcaac
     6901 gccctgcaac tgctgcattt ctggaatgcg gagattccgc tcgcccaggg agccgctgtg
     6961 ccgctggtac gcgcaccgcg tgatgcagca tctgtgcacg gcgaatcggg aatggctggc
     7021 tacgactttg ttgagcacaa ccgaaagcca atcgggatac cagcgtttct ggcgattcgt
     7081 gatgccctga tgcgtgcacc agagcctgtt accctggtgg ccattggccc gttaaccaat
     7141 attgcgctgt tactttcaca atgcccggaa tgtaaaccgt atattcgtcg tctggtgatc
     7201 atgggcggtt cttctggtcg cggcaactgt acaccaaatg ccgagtttaa tatcgctgcc
     7261 gatccagaag cggctgcctg tgtcttccgt agcggaatcg aaatcgtcat gtgcggtctg
     7321 gatgtcacca atcaggcaat attaacccct gactatctcg ctactttgcc ggagttgaat
     7381 cgcaccggaa aaatgcttca tgcactgttt agccactacc gtagcggcag tatgcaaagc
     7441 ggtctgcgaa tgcacgatct ctgcgccatc gcctggctgg tgcgcccgga cttattcacc
     7501 ctcaagccgt gttttgtcgc ggtagaaact caaggcgaat ttacctcagg cacgacggtg
     7561 gttgatatcg acggttgcct gggcaaacca gccaatgtaa aggtggcatt ggatctggat
     7621 gtgaaaggct tccagcagtg ggtggctgag gtgctggctc tggcgtcgta acctgtcaca
     7681 tgttatcggc atgcagtcat tcatcgactc atgcctttca ctgatatccc tccctgttta
     7741 tcattaattt ctaattatca gcgtttttgg ctggcggcgt agcgatgcgc tggttactct
     7801 gaaaacggtc tatgcaaatt aacaaaagag aatagctatg catgatgcaa acatccgcgt
     7861 tgccatcgcg ggagccgggg ggcgtatggg ccgccagttg attcaggcgg cgctggcatt
     7921 agaaggcgtg cagctgggcg cggcgctgga gcgcgaagga tcttctttac tgggcagcga
     7981 cgccggtgag ctggctggtg cagggaaaac aggcgttacc gtgcaaagca gcctcgatgc
     8041 ggtaaaagat gattttgatg tgtttatcga ttttacccgt ccggaaggta cgctgaacca
     8101 tctcgctttt tgtcgccagc atggcaaagg gatggtgatc ggcactacgg ggtttgacga
     8161 agccggtaaa caggcgattc gtgacgccgc cgccgatatt gcgattgtct ttgccgccaa
     8221 ttttagcgtt ggcgttaacg tcatgcttaa gctgctggag aaagcagcca aagtgatggg
     8281 tgactacacc gatatcgaaa ttattgaagc acatcataga cataaagttg atgcgccgtc
     8341 aggcaccgca ctggcaatgg gagaggcgat cgcccacgcc cttgataaag atctgaaaga
     8401 ttgcgcggtc tacagtcgtg aaggccacac cggtgaacgt gtgcctggca ccattggttt
     8461 tgccaccgtg cgtgcaggtg acatcgttgg tgaacatacc gcgatgtttg ccgatattgg
     8521 cgagcgtctg gagatcaccc ataaggcgtc cagccgtatg acatttgcta acggcgcggt
     8581 aagatcggct ttgtggttga gtggtaagga aaatggtctt tttgatatgc gagatgtact
     8641 taatctcaat aatttgtaac cacaaaatat ttgttatggt gcaaaaataa cacatttaat
     8701 ttattgatta taaagggctt taat
//
LOCUS       DAZOYL010000163         8654 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZOYL010000163 DAZOYL010000000
VERSION     DAZOYL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.163"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..2893)
                     /gene="nifJ"
                     /locus_tag="ACHUOY_002724"
     CDS             complement(<1..2893)
                     /gene="nifJ"
                     /locus_tag="ACHUOY_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR0683464.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDG"
     gene            3167..3433
                     /locus_tag="ACHUOY_002725"
     CDS             3167..3433
                     /locus_tag="ACHUOY_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR0683465.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(3430..3852)
                     /gene="hslJ"
                     /locus_tag="ACHUOY_002726"
     CDS             complement(3430..3852)
                     /gene="hslJ"
                     /locus_tag="ACHUOY_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR0683466.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(3963..4952)
                     /gene="ldhA"
                     /locus_tag="ACHUOY_002727"
     CDS             complement(3963..4952)
                     /gene="ldhA"
                     /locus_tag="ACHUOY_002727"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0683467.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5160..7799
                     /locus_tag="ACHUOY_002728"
     CDS             5160..7799
                     /locus_tag="ACHUOY_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR0683468.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            7796..7981
                     /locus_tag="ACHUOY_002729"
     CDS             7796..7981
                     /locus_tag="ACHUOY_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR0683469.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            7986..8315
                     /locus_tag="ACHUOY_002730"
     CDS             7986..8315
                     /locus_tag="ACHUOY_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR0683470.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(8487..>8654)
                     /locus_tag="ACHUOY_002731"
     CDS             complement(8487..>8654)
                     /locus_tag="ACHUOY_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0683471.1"
                     /translation="NRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFG
                     MTPGEYRRKFR"
BASE COUNT         2141 a         2100 c         2281 g         2132 t
ORIGIN      
        1 agccatcacc accaatcagc cagattgatt tttccaccag tgcatctgca tcacgtagca
       61 gttcatgtgc ttcggcaaca tcgttgagtt gctggcgtaa agctgcgacc tgttctcgac
      121 gaacctctgg cgtagcgtct gatttcaacg ccgtcagtaa ttccgccggg attttatcgg
      181 caaattgatc cagcagacgc agcacgcgga cacggtgttg atcgaccgtc aggcggaaac
      241 caaggccaaa ttcggcatta tcttcaaata gagagttcgc ccatgccggc ccacgaccgt
      301 tggcatcggt ggtatacggt gtagagggca ggttaccgcc ataaattgaa gaacagccgg
      361 tggcgttagc gatcaacatc cggtcgccat agagctgagt cagcaattta atatacggcg
      421 tctcgccaca accggagcaa gcaccggaat attcaaacag cggtgtaatc agctgcgatg
      481 tacgaatatc aatacgttcc agtttgctac ggtcgatttc tggcaggttg aggaagaaat
      541 cgtaattgat tttctcttct tcgacatgtt ccaggcgcga catcatattg atggctttaa
      601 tctctggatt ctgacggtct ttcgccgggc aaacttcgac gcacaggtta caaccggtgc
      661 aatcttccgg agccacctgc aagacatatt tctgcccgcg catatcacgc gatttcacat
      721 ccagcgaatg caggctggca ggggcgtttt ccatcgcttc gggtggtacc acttttgcgc
      781 gaatagctga gtgtgggcaa gctgcgacgc agtggttaca ttgggtacag agttcctctt
      841 tccagatggg gatctcttcg gcgatattgc gtttttccca gcgagtggtg cccatcggcc
      901 aggtgccgtc tggcggcagc gccgaaacgg ggagggcatc accaagccct gcgagcatcg
      961 ctgcggttac ggttttcacg aaatcagggg cggcatcgga aaccactggc ggtcgattgg
     1021 cgctgtgcgg atttaccggt tgcaacggaa cttcttctac ggattcacgc gccagcgcca
     1081 gagcctgcca gttgcgttcc accagatcct ggcctttgct actgtaactt ttggcaatcg
     1141 caccctgcaa ttctgcgagg gcgctatcgc caggcagaat ttgcgtcaga tggaaaaaag
     1201 ccatctgcat gacggtatta atacgggccg ccaggccaca tttgcgggcg attttcgccg
     1261 cgttaatcac atagaagcgc gcttttttct ggtttaacac ggcctgaact tcttgcggca
     1321 agcgcgacca cacttcatct gcgctgtacg gcgtgttgag caggaaaatg ccgccaggtt
     1381 ttaaacgctc agccatctga tatttatcga taaactgcaa ctggtggcag ccaacaaaat
     1441 cagcctggga aatgagataa gcggaacgaa tcggctgttc gctcacgcga aggtgagaaa
     1501 ccgtcaggcc gcccgctttt ttggagtcgt aaacaaaata gccctgtgcg taccacggcg
     1561 tggaattacc gataatcttg atattgtttt tggtcgcgga aacgctgcca tcactaccaa
     1621 ggccataaaa caaggcttca agtttcgccg agtttggctg ggtgttttcc ggcaacggca
     1681 gggacagatt ggttacatca tcgtaaatac caaccgtaaa gcgcgctttc ggtttagccg
     1741 cgttgagctc ggcaaatacc gccagtacac agtccgggcc aaattctttg gatgaaagac
     1801 catagcgccc accaatgaca cggggcagag tttcgcgctt gccattatta aaggcttctg
     1861 ccagtgcggt catcacatcc agatagagcg gttctgcctg ggcaccgggt tctttggttc
     1921 tgtccagtac cgccacgctg cgtacggatc ccggtagagt ttgtagcaag tgtttggctg
     1981 agaaggggcg gtacaggcga actttcagca cgccgacttt ttcgccacgg gttagcaatt
     2041 catcaaccac ttcttcacag gtgccaatgg ctgagcccat caggataatc acccgttcag
     2101 cttgcggatg cccgtaatat tcaaacggct gatactgacg acctgtcgcg gcagagaaat
     2161 cattcatcgc ctgttcaaca tggtcataga ctgcgttgta ccaggggttg gtggcttcgc
     2221 gagactggaa ataagtgtca ggattggcgg acgtaccacg gatcaccgga tgttccgggt
     2281 tgagcgcccg ggcgcgatga gcatcaattt cggcctgcgg catgagatca agaatcgtgt
     2341 catcggccag cgggacaatt ttgttgattt cgtgggacgt gcggaaacca tcaaagaaat
     2401 gaataaatgg cacgcggctt ttcagcgtcg cgatgtgcga aatgagagca aagtcttgcg
     2461 cttcctggac gtttgccgca cacaacatcg cgcaacccgt ctggcgcacc gccatgacgt
     2521 cggaatgatc gccaaaaata gagagtgcat gtgtggcaac ggtacgtgcc gctacatgca
     2581 ggacaaacgg tgtgagttcg cctgccagtt tgtacagtgt cgggatcatc agcagcaaac
     2641 cctgcgatga cgtaaacgat gttgaaaggg cacccgtctg caaagcgcca tgcacggtag
     2701 cgatagcacc cgcttccgac tgcatttcaa ccacgcatgg tgtgtctccc caaacgttct
     2761 tcaagccgtt tccggcccag gcatcagcct gttctgccat cgtggaactg ggggtaatag
     2821 ggtagatggc gataacttca ctggtgcgaa acgccaccga agcaaccgcg ccattaccat
     2881 caatagtaat catatgacac ccttacattg cgcaaatgag gggcgcacga aattgctgcg
     2941 cgcccagtag taatccttca attttagcaa atggctttct tctgcatttt cgcttttgtg
     3001 tcccccacat cagcgtaatg aatgttttga tcaaacagag ggcaaaaaaa tagccataaa
     3061 aaagtaaaaa tgcggggcaa cgcgcaggac agctctcgac gccgccctct gtgctgccta
     3121 ttatttatgg cggtgtcgtt tggcttgaag atcagaggaa gaagagatgc gagcagcgtt
     3181 ttgggtaggg tgtgccgctt tattgttgtc ggcgtgtagt agtgaacctg ttcagcaggc
     3241 gactgcggcg cacgtagcgc caggtttaaa agcgtcgatg tccagtagtg gagaagcaaa
     3301 ttgtgcaatg atcggcggtt cgctttctgt tgcccgtcaa ctggatggta cggcgattgg
     3361 gatgtgtgca ttacccaacg gcaaacgctg tagcgaacag tcacttgccg ccgggagctg
     3421 tggcagctat taattcatta aatccgccag cttataagtt aatgtctgtt tcgcggtcgc
     3481 cagcgttaac tggttcgcgg tcagatccac ttgtgcacct tctttcagca tttcgctaat
     3541 ggtgttatcg agttcattaa gctgcgggtt agcgcacatc atacgggtca ttgccagccc
     3601 tttggctgtc agttcaccat tagacagttt gccttcaccg ctaaagcggt tacacatgct
     3661 gccggaaatc atcatttttt caccaaagct gatttctggc ggatttttat cgctggtcac
     3721 gggcttaccg tttacgcttt ccagcacaaa gcgatgatgc tgtagctgtt ctggcgtaac
     3781 agcaattttg tcattactta cacatcccgc catcagcagg cttagcgcaa caaacgcggc
     3841 tactttcttc attgtggttc tcaattacag tttctgactc agggctattt taagaataga
     3901 agatgaaagg tctttgggga ttatctgaat aagctcccct ggaatgcagg ggagcggcaa
     3961 gattaaacca gttcgttcgg gcaggtttcg cctttttcca gattgcttaa gttttgcagc
     4021 gtagtctgag aaatactggt cagagcttct gctgtcagga atgcctggtg cccggtaaac
     4081 agcacgttgt ggcaggcaga caggcgacgg aatacgtcat cctggatcac gtcgttggat
     4141 ttatcttcaa agaatagatc gcgttcgttc tcatagacgt ccatacccaa cgacccaatt
     4201 ttctgatttt tcagcgcttc aattgctgcc tgagaatcaa tcaatgcacc gcgactggta
     4261 ttgacgatca tcacgccatt tttcatctga tcgaaggcgg cttcgttcaa cagatggtag
     4321 ttttccggtg tcagcgggca gtgcagagag ataacgtctg attcagagaa cagggttggc
     4381 agatcgacat actccacacc gagttccagc gccgctgcac ttggatacgg atcgaacgcc
     4441 agcagacgca taccaaaacc tttcagaatg cgcagcatcg ccacaccgat tttaccggta
     4501 ccgataacgc ctgccgtttt gccatacata gtaaagccgg tcagaccttc cagagagaag
     4561 ttagcgtcac gggtacgctg atacgcgcgg tgaatacggc ggttcagcgt catcatcata
     4621 ccgatggcgt gttcagcaac ggcctctgga tcataggctg gaacacggac tactttcagc
     4681 cccagttctt ttgccgcgtc aaggtcgacg ttattgaaac cggcacagcg cagggcgata
     4741 tatttaacgc cgtgcttttt cagctcttcc agcaccgggc ggctgccgtc atcgtttacg
     4801 aaaatacata ccgcttcgca gccattggca gttttagcgg ttttttccgt cagcagaaag
     4861 tcaaaaaatt ccagctcaaa gccaaaggac tcgttcacct gttgcaggta cttcttgtcg
     4921 tactgttttg tgctataaac ggcgagtttc ataagacttt ctccagtgat gttgaatcac
     4981 atttaagcta ctaaaaatat tttacaaaat ttcaaatata attgaaagct atggcgatat
     5041 tgaaaaattc atcaacaaca atgcttagtg taggcgcaac cttcaactga acggttaaac
     5101 atgccacaat acccgtattg aatgcttaat ttttcgctaa atcaggatat taactaccca
     5161 tgctgggtaa atataaagcc gttctcgcgc tgttattact gattattctt gtgccgttga
     5221 cgctgctgat gacgctcggg ctgtgggttc ccacgctggc gggcatctgg ctaccgctcg
     5281 ggacacgtat tgcattagat gaaagcccac gcattacgcg taaaggttta atcattcccg
     5341 atctccgtta tctggtggga gattgtcagc ttgcgcatat caccaacgcc agcctttcac
     5401 atcccagccg ctggttattg aacgtcggca tggtagaact tgattctgct tgtctggcga
     5461 aattgccgca gacggagcaa tcgccagctg ctccaaaaac cctcgcgcag tggcagtcca
     5521 tgctgcctaa cacctggatc aatatcgata aactgatttt ttctccctgg caggaatggc
     5581 agggaaaact ctctctcgca ttaacctctg atattcagca actgcgttat cagggcgaaa
     5641 aagttaaatt tcaaggccag ctgaaagggc aacaacttac agtcagcgaa ctggatgtca
     5701 tcgcgtttga aaatcagccg ccggttaaac tggtggggga atttactatg ccgctcgtgc
     5761 cggatggact tcctgtaagt gggcatgcta ctgcgacgtt aaacttgccg caggaaccgt
     5821 cactggtgga tgcagagctg gactggcagg aaaatagcgg gcaattgatt gtgctggcac
     5881 gggataacgg cgatccgttg ctcgatttgc cgtggcaaat tactcgtcaa caattgaccg
     5941 taagcgatgg tcgctggagt tggccgtatg caggttttcc tttgagtggc cgactgggtg
     6001 tcaaagtcga caactggcag gcagggcttg agaacgctct gatcagcgga cgactgagtg
     6061 tgctgaccca ggggcaagcg ggtaaaggca acgcggtgct aaattttggc ccaggaaaat
     6121 taagcatgga taacagccag ctgcctctgc agctgaccgg tgaagcgaaa caggcggacc
     6181 tcattttata tgcccgctta cccgcgcagc taagtggaag tctgactgac ccaacgctgg
     6241 cctttgagcc aggcgcgtta ttacgttcga agggaagagt catcgattcg ctggacatcg
     6301 atgaaatccg ctggccttta gcgggtgtaa aagtcaccca acgtggtgtt gacggacgtt
     6361 tgcaggccat tttgcaggcg catgaatatg aactgggcga tttcgtgctg catatggatg
     6421 ggctggcgaa tgattttctc cctgacgcgg gccgctggca gtggcgctac tggggaaaag
     6481 gcagttttac accgataaat gccacctggg atgtcgcagg aaaaggtgaa tggcatgaca
     6541 gcacgattac gctgaccgac ctctccaccg gtttcgacca gttacaatac ggtacgatga
     6601 cggtagaaaa gccgcgatta attctcgaca agcccgtcgt ctgggtacgt gacgcacagc
     6661 atccctcctt cagcggcgcg ctgtcactgg acgccgggca aacgctgttc actggcggca
     6721 gtgtgttacc gccatcaacc ttaaaattta gcgtcgatgg gcgcgatccg acgtatttcc
     6781 tctttaaagg cgatttacat gctggtgaga ttggcccggt tcgggtaaat ggtcgctggg
     6841 acggtattcg tctgcgcggt aacgcctggt ggccaaaaca atcactgact gtattccagc
     6901 cgctggtgcc acccgactgg aagatgaact tacgcgatgg ggaactgtat gctcaggttg
     6961 cattttctgc tgcgcctgaa caaggattcc gcgcgggagg acacggcgtg ttgaaaggcg
     7021 gtagtgcctg gatgccagat aatcaggtta acggtgtcga ttttgtcctg cctttccgtt
     7081 ttgccgatgg agcctggcat ctggggactc gcggccccgt tacgttgcga attgccgaag
     7141 tgattaatct ggtgacagcg aaaaatatta cggctgattt gcaagggcgt tatccgtgga
     7201 ctgaagaaga acccttgctg ttgactgatg ttagcgtcga tgtgttaggc ggtaacgtac
     7261 tgatgaaaca attacgtatg ccgcaacatg acccggcgct gttgcggctg aataatctct
     7321 catccagcga actggttagc gccgtcaatc cgaaacaatt cgccatgtcc ggggcattta
     7381 gtggtgcatt gccgttatgg ctaaacaacg aaaaatggat agtgaaagat ggttggctgg
     7441 caaatagcgg gccaatgacc ttgcggctgg ataaagacac cgcagatgcg gtggtgaaag
     7501 acaatatgac tgcgggttca gcaattaact ggttgcgcta tatggaaatt agccggtcat
     7561 cgacaaaaat taacttagat aatctcggtt tattaaccat gcaggccaac attacaggta
     7621 ccagtcgcgt tgatggtaaa agcggtacgg taaaccttaa ttaccatcat gaagagaata
     7681 tttttacgct gtggcgcagt ttacgctttg gcgataatct ccaggcatgg ttggagcaga
     7741 acgcacgtct gccgggaaat gactgtccgc aaggaaaaga gtgtgaggaa aaacaatgaa
     7801 aattttactg gctgcgttga cgtcatcttt tatgctggtt ggctgtacgc cgcgtattga
     7861 agtcgctgcg cccaaggaac cgatcactat caatatgaac gttaaaattg agcatgagat
     7921 catcatcaag gcagacaaag atgtcgaaga gctgcttgaa actcgtagcg atcttttctg
     7981 aggcgatgat gaagaaaaca ttacttcttt gtgcgtttct tgttgggctg gtaagcagca
     8041 atgtaatggc attgactctg gatgaagcca gaactcaggg gcgggtaggt gaaacatttt
     8101 atggttatct ggttgcgctg aaaacggatg ctgaaacaga gaaattagta accgacatta
     8161 atgccgaacg taaagcgagt taccaacaac tggcaaagca aaataatgtg tcggtagatg
     8221 atatcgcgaa actcgccggg caaaagctcg tagctcgggc caaaccgggg gaatatgtac
     8281 aagggataaa cggtaaatgg gtgcgaaaat tttaatagca aaaccgcaat atcgggataa
     8341 cgctacagga gaaagcgatt gatctatttt cctgaaacaa ggtgaatatt caaaaactcc
     8401 tgtcaaattg ccttttgccc tgaaaaatgc atagtcaaat atctgtttta actaattggc
     8461 gttgcagtac acacaacgcg aattagttaa cggaatttac gtcgatactc gcctggcgtc
     8521 atcccaaagc gttgcttaaa taccgttgaa aaatgactct ggtcagaaaa tccccaatga
     8581 aagccgatgc ctgccagttt ttcatcatct gcggcatggc gaatcgcatc tgcacaaaaa
     8641 tcgagacgac ggtt
//
LOCUS       DAZOYL010000164         8633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZOYL010000164 DAZOYL010000000
VERSION     DAZOYL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.164"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="ACHUOY_002732"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="ACHUOY_002732"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR0683472.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="ACHUOY_002733"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="ACHUOY_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR0683473.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHUOY_002734"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHUOY_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR0683474.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHUOY_002735"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHUOY_002735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR0683475.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHUOY_002736"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHUOY_002736"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR0683476.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHUOY_002737"
     CDS             complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHUOY_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR0683477.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHUOY_002738"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHUOY_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR0683478.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2247 c         2051 g         2179 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taa
//
LOCUS       DAZOYL010000165         8573 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZOYL010000165 DAZOYL010000000
VERSION     DAZOYL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.165"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(151..1815)
                     /gene="glnS"
                     /locus_tag="ACHUOY_002739"
     CDS             complement(151..1815)
                     /gene="glnS"
                     /locus_tag="ACHUOY_002739"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR0683479.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2075..4021)
                     /gene="nagE"
                     /locus_tag="ACHUOY_002740"
     CDS             complement(2075..4021)
                     /gene="nagE"
                     /locus_tag="ACHUOY_002740"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR0683480.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4354..5154
                     /gene="nagB"
                     /locus_tag="ACHUOY_002741"
     CDS             4354..5154
                     /gene="nagB"
                     /locus_tag="ACHUOY_002741"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0683481.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5214..6362
                     /gene="nagA"
                     /locus_tag="ACHUOY_002742"
     CDS             5214..6362
                     /gene="nagA"
                     /locus_tag="ACHUOY_002742"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0683482.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6371..7591
                     /gene="nagC"
                     /locus_tag="ACHUOY_002743"
                     /gene_synonym="nagR"
     CDS             6371..7591
                     /gene="nagC"
                     /locus_tag="ACHUOY_002743"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR0683483.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7639..8391
                     /gene="nagD"
                     /locus_tag="ACHUOY_002744"
     CDS             7639..8391
                     /gene="nagD"
                     /locus_tag="ACHUOY_002744"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR0683484.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8442..8573)
                     /locus_tag="ACHUOY_002745"
                     /pseudo
     CDS             complement(8442..8573)
                     /locus_tag="ACHUOY_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2098 a         2260 c         2183 g         2032 t
ORIGIN      
        1 ggcctacaaa agcacgcaaa ctcaatatat tgcagagatc atgtaggcct gataagcgta
       61 gtgcatcagg caatttagcg tttgaaactg tttgtctgtt ccttgccgga tgcgacgtaa
      121 acgccccatc cggcatagcg aaacttaaaa ttactcgcct actttcgccc aggtatcacg
      181 cagcccaacg gtgcggttaa ataccggttt ttccgccgta gaatggcggc tatcgaggca
      241 gaagtaacct tcacgctcaa actggaatgc tttacccgca accgcatctt tcagcgacgg
      301 ttcagcaaag ccctgtttga tcaccagcga ttccgggtta atcaccgaca ggaaatcatc
      361 cgcagcacca gggttcggca cgctgaacag acggtcgtac agacggattt caaccggcag
      421 cgcatgtgcc gcgctcaccc agtgaataac acctttgact ttacgaccat ctgccggatc
      481 tttgcttaag gtgtcggcgt cataagtaca gaagatggtg gtgatattgc cttcggcatc
      541 tttctccacg cgttctgcct taatgacgta agcattacgc agacgcactt ctttacccag
      601 caccagacgt ttgtactgct tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat
      661 ctcaccgcta aacggcacct ggcggctacc catttccggt ttgttcggat ggttcggcat
      721 ggtaaccatt tcgccttcgc cctgatagtt ttcgataacc agtttcaccg gatcgataac
      781 cgccattgcg cgcggggcgt tttcgttgag atcttcacgg atgcaggatt ccagcgacgc
      841 catctcaatg gtgttgtcct gcttggtcac gccgatgcgt ttgcagaact cacgaataga
      901 agcagcagtg taaccacgac gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca
      961 gccttcaacg tgcttgtcgg tcaccagcag gttcagctta cgcttggaca tcacagtgta
     1021 ttccagattc aggcgcgaga attcgtactg gcgcgggtga acaggaatgg tgatgttgtc
     1081 cagcacccag tcgtacaggc gacggttgtc ctggaactca agcgtacaca gagagtgcgt
     1141 aataccttcc agggcatcgc tgatgcagtg ggtgaagtcg tacatcgggt agatgcacca
     1201 cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat
     1261 cacgataaac ggtgaagcca tgtcgatttt cgcacgcagg caggctttac cttcttcaaa
     1321 accaccggca cgcatttttt cgaacagcgc caggttctct tcaacgctgc ggtcgcggta
     1381 cgggctgttt ttacccggtt gcgtcagggt gccgcggtat tcgcggatct gttccggcgt
     1441 cagttcatca acgtacgcca ggcctttatt gatcagttcg atcgcatagg cgtggagctg
     1501 atcaaaataa tcggaggagt aacggacgtt accagaccag tgaaaaccta accactctac
     1561 gtcgtttttg atcgactcaa catactcgat atcttctttt accgggttag tgtcgtcgaa
     1621 acgcaggttg cactggcctt tatagtcctg ggcgatcccg aagttcaggc agatagattt
     1681 cgcatggcca atatgcagat agccattcgg ctccggcggg aaacgggtgt gtactgtggt
     1741 gtgcttacca ctggccagat cttcatcgat gatctgacgg ataaagttag tcgggcgggc
     1801 ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct
     1861 tataagcggg acaggctgac aactgttagt tagcgaaaat cgaaaatctc actgagatta
     1921 tggggatgat tgatgaaaaa aaaaagcggc agagaagatc tccaccgctt ttgccagtcc
     1981 cgcgtcattg ttggatgcgc gtcttatcca accaggactg agcttttgta ggcctgataa
     2041 gacgcgtcaa gcgtcgcatc aggcataaag cagattactt tttgatttca tacagcggtg
     2101 tttgacccgc cacaacattg ccctgagctt taatgatcaa gccactgaaa tcgtcgatat
     2161 tgctgcaaac caccgggcta atcatcgaac gggcgttagc gttcaggtaa tccagatcca
     2221 tttccagaat cggttgccct gcgctcacct gcgcgccctc ttccaccaga cgtttaaagc
     2281 ctttaccttc cagcgctacg gtgtcgatac ccatgtggac gacaatctcc gcgccttttt
     2341 cggtttctag gcagaacgcg tggttggtgt tgaagatttt cacgattgtc cctgcggctg
     2401 gtgatacgac gattttatct gtcggtttca ccgccacacc gtcacccacc gctttgctgg
     2461 cgaatgcttc gtcagaaacc tgatccagtg ccacgacatc accggtaatc ggcgatacca
     2521 gctccgcgat agataccgcg tttggtacag cctgcggttt tgctacagga gcggcagttg
     2581 ccggagttgc ttcagctgac gcagctgcta ccgggccacg ggcaacgact ttcttcatcg
     2641 catcgccaat ggattctgct ttcgcgccaa caatcacctg aatggtctgt ttgttcagtt
     2701 ttactacacc agaagcaccc agacgtttac acatcgcatc gttgacgcga gctgagtcag
     2761 ccacggtaag gcgcagacgg gtaatacagg cgtcaatcgc tttcaggttg tcagtaccgc
     2821 caaccgcagc aatatagttg gttgccagtt gagtcagacc ttcttcagta ttgctgttag
     2881 cctcttcagt tacgatctcg tcttctttat cttcacgacc cggcgttttc aggttgaaca
     2941 tgcggataac caaactgaac accacgaagt agatagcgaa gaagacaaca cccatcacca
     3001 gcagcatcca gacgttctgg ctggcggccg gcaggttata catcaacgcg tagtcgatag
     3061 cccccgcaga gaaagagaag ccagcatgga tacccagcag cgttgccaca aacaggctga
     3121 taccggtcag cagtgcgtgc aggaggtaca gcagcggagc aaggaacatg aacaggaatt
     3181 ccagcggctc agtcacaccg gtcaggaacg cagtaacagc aacagaaagc agcatcccgc
     3241 caaccatcgg acgacgctct ttcggtgctg cgaagtacat cgccagcgcc gcacccggca
     3301 gaccgaacat catgatcggg aagaagccgg acatgaacat ccccgcggtg ccgtcaccag
     3361 cgtagaaacg gttgatgtcg ccgtggaaaa ccgtacccgc cgcgttggtg aattcaccaa
     3421 tctggaacca ggcgatggtg ttcagcacct gatgcagacc ggttgggatc agcagacggt
     3481 tgatgaaacc aaagataccg gaacccagcg cgcctgcaga aacgatccac tcgccgcctg
     3541 catggatagc gtgctgtact ggcggccaga cgtaaccaaa aatggccgcc agcaccaggc
     3601 agaaaaagcc ggtggcaatc ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg
     3661 gcagtttaat atcggaccaa cggttatagg ctgcgccacc aaccagaccg gtaatgatac
     3721 ccgccagtac acccatgtta atttctgggt tgatggtcac catcgctttg gttaacacaa
     3781 agtaacctac cgcacccgcc agcgccgccg cacctgcgct gtctttcgac cagctggatg
     3841 ccacaccgat ggcgaagatt aatgcgaggt tatcaaaaat cgcaccgccc gcctgggcaa
     3901 taaacgcaac gttaagtaaa tctggctgac cgaatcgcag caacagtgcc gccaccggca
     3961 gcaccgcgat agggagctgt aacgccctac cgagtcgctg gaaaaaacct aaaatattca
     4021 tcttattccc cctacgagaa ccctatttgg ctcgtttaaa gccgtatttt tattttgctg
     4081 caaattgtac tgccgatgtt ctgtaatcag attgttagat catctgctac agagtgtgtg
     4141 aaaatttaat tcgtatcgca aattaaacgc gtgccttttg tgagttttgt caccaaatat
     4201 cgttatcatc actccctttt actggctaaa gcagaaaact tattttatca ttcaaaaaat
     4261 caggtcggat tgacgcctgt ctgcgcaaat ccaggttacg cttaaagatg cctaatccgc
     4321 caacggctta cattttactt attgaggtga ataatgagac tgatccccct aactaccgct
     4381 gaacaggtcg gcaaatgggc tgctcgccat atcgtcaatc gtatcaatgc gttcaaaccg
     4441 actgccgatc gtccgtttgt tctgggcctg ccgactggcg gcacgccgat gaccacctat
     4501 aaagcgttag tcgaaatgca taaagcaggc caggtcagct ttaagcacgt tgtcaccttc
     4561 aacatggacg aatatgtcgg tctgccgaaa gagcatccgg aaagctacta cagctttatg
     4621 caccgtaatt tcttcgatca cgttgatatt ccagcagaaa acatcaacct tctcaacggc
     4681 aacgccccgg atatcgacgc cgaatgccgc cagtatgaag aaaaaatccg ttcttacgga
     4741 aaaattcatc tgtttatggg cggtgtaggt aacgacggtc atattgcatt taacgaaccg
     4801 gcgtcttctc tggcttctcg tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg
     4861 aactctcgtt tctttgataa cgatgtcaat caggtgccaa aatatgccct gactgtcggt
     4921 gttggtacat tgctggatgc cgaagaagtg atgattctgg tgctgggtag ccagaaagca
     4981 ctggcactgc aggctgcagt tgaaggttgc gtgaaccaca tgtggaccat cagctgtctg
     5041 caactgcatc caaaagcgat catggtgtgc gatgaacctt ccaccatgga gctgaaagtt
     5101 aagactttaa gatatttcaa tgaattagaa gcagaaaata tcaaaggtct gtaattgtta
     5161 tccctgccct ggctccttgc tcagggcaat attttttaaa atcggggggc agaatgtatg
     5221 cattaaccca gggccggatc tttaccggcc atgaatttct tgatgaccac gcggttgtta
     5281 tcgctgatgg cctgattaaa agcgtctgtc cggtagcgga actgccgcca gagatcgaac
     5341 aacgttcact gaacggggcc attctctccc ccggttttat cgatgtgcag ttaaacggct
     5401 gcggcggcgt acagtttaac gacaccgctg aagcggtcag cgtggaaacg ctggaaatca
     5461 tgcagaaagc caatgagaaa tcaggctgta ctaactatct gccgacgctt atcaccacca
     5521 gcgatgagct gatgaaacag ggcgtgcgcg ttatgcgcga gtacctggca agacatccga
     5581 atcaggcgtt aggtctgcat ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc
     5641 ataatccgaa ttttgtgcgt aagcctgatg ccgcgctggt cgatttcctg tgtgaaaacg
     5701 ccgacgtcat taccaaagtg accctggcac cggaaatggt tcctgcggaa gtcatcagca
     5761 aactggcaaa tgccgggatt gtggtttctg ccggtcactc caacgcgacg ttgaaagaag
     5821 cgaaagccgg tttccgcgcg gggattacct ttgccaccca tctgtacaac gcgatgccgt
     5881 atattaccgg tcgtgaaccg ggcctggcgg gcgcgatcct cgacgaagct gacatttatt
     5941 gcggtattat cgctgatggc ctgcatgttg attacgccaa cattcgtaac gctaaacgcc
     6001 ttaaaggcga caaactgtgt ctggttaccg acgccaccgc gccagcaggt gccaacattg
     6061 aacagttcat ttttgcgggt aaaacaatat actaccgtaa cggactttgt gtggatgaga
     6121 acggtacgtt aagcggttca tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac
     6181 attgcggtat cgcactggat gaagtgctgc gtatggcgac gctctatccg gcgcgtgcga
     6241 ttggcgttga gaaacgtctc ggcacactcg ccgcaggtaa agtagccaac ctgacctcat
     6301 tcacacctga ttttaaaatc accaagacca tcgttaacgg taacgaggtc gtaactcaat
     6361 aagagaaagt atgacaccag gcggacaagc tcagataggt aatgttgatc tcgtaaaaca
     6421 gcttaacagc gcggcggttt atcgcctgat tgaccagtac gggccaatct cgcggattca
     6481 gattgccgag caaagccagc ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat
     6541 cgaacgcggg ctgatcaaag aagttgatca gcaggcctcc accgggggcc gccgcgctat
     6601 ctccatcgtc accgaaaccc gcaatttcca cgcaatcggc gtacggcttg gtcgtcacga
     6661 cgccaccatc accctgtttg atctcagcag caaagtgctg gcagaagaac attacccgct
     6721 gccggaacgt acccagcaaa cgctggaaca tgccctgttg aatgccattg ctcagtttat
     6781 tgatagctac cagcgcaaac tgcgcgagct gatcgcgatt tcggtgatcc tgccagggct
     6841 tgttgacccg gacagcggca aaattcatta catgccgcat attcaggtag aaaactgggg
     6901 gctggtagaa gctctggaag aacgttttaa agtgacctgt ttcgttggtc acgatatccg
     6961 tagtctggcg ctggcggagc actacttcgg tgcaagtcag gattgcgaag actccattct
     7021 ggtgcgtgtc catcgcggaa ccggggccgg gattatctct aacgggcgca tttttattgg
     7081 ccgcaacggt aacgtcggtg aaattggcca tattcaggtc gaaccgctgg gtgaacgctg
     7141 ccactgcggc aactttggct gcctggaaac tatcgccgcc aacgctgcca ttgaacaacg
     7201 ggtgttgaat ctgttaaagc agggctacca gagccgcgtg ccgctggacg actgcaccat
     7261 caaaactatc tgcaaagccg cgaacaaagg cgatagtctg gcgtcggaag taattgagta
     7321 tgtcggtcgt catctgggta aaaccatcgc cattgctatc aacttattta atccgcaaaa
     7381 aattgttatt gccggtgaaa tcaccgaagc cgataaagtg ctgctccctg ctattgaaag
     7441 ctgcattaat acccaggcgc tgaaggcgtt tcgtactaat ctgccggtgg tacgttctga
     7501 gctggatcac cgctcggcaa tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg
     7561 tattttgctc cagcatttgc tggaaaatta atgtgctttt atagtggcgc ttattgttgt
     7621 caatattctg ggtagtccat gaccattaaa aatgtaattt gcgatatcga cggcgtgctg
     7681 atgcacgata acgtcgccgt accgggtgca gcggaatttt tgcacgggat tatggataaa
     7741 ggcctgccgc tggtgttgct gaccaactat ccttcgcaga ccgggcaaga tctggcgaac
     7801 cgctttgcca ctgccggtgt cgatgtgccg gatagcgtgt tttatacctc tgcgatggcg
     7861 accgccgatt tcctgcgtcg ccaggaaggc aagaaagcat atgtggtggg cgaaggcgca
     7921 ctgattcatg aactgtacaa agccggtttc actattaccg atgtgaaccc tgatttcgtg
     7981 attgttggcg aaacgcgttc ctacaactgg gacatgatgc ataaagcggc ctatttcgtc
     8041 gctaacggtg cacgctttat cgccaccaac ccggacaccc acgggcgcgg tttttatccc
     8101 gcttgtggcg cgttgtgtgc agggattgag aaaatctccg ggcgcaaacc gttctatgtt
     8161 ggtaagccca gcccgtggat catccgcgca gcattaaaca aaatgcaggc gcattcggaa
     8221 gaaacggtga ttgtcggcga taacttgcgc accgacattc tggcgggctt ccaggcaggt
     8281 ctggaaacga ttctggtgct ttctggtgtt tcatcgctcg acgatatcga cagtatgccg
     8341 ttccgcccaa gctggattta cccgtcagtc gctgaaatcg acgttatctg aataccaccc
     8401 acgtctgcgg acgtggttgg ctctgatgac aaacgccaaa ctgcctgatg cgctacgctt
     8461 atcaggccta cgcagctcct gcaatatatt gaattttcat gcttttgtag gccggataag
     8521 gcgttcacgc cgcatccggc atgaacaaat cgcactttat cagcaatttg gcc
//
LOCUS       DAZOYL010000166         8533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZOYL010000166 DAZOYL010000000
VERSION     DAZOYL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.166"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="ACHUOY_002746"
     CDS             complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="ACHUOY_002746"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR0683485.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHS"
     gene            complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="ACHUOY_002747"
     CDS             complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="ACHUOY_002747"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR0683486.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1322..2986)
                     /locus_tag="ACHUOY_002748"
     CDS             complement(1322..2986)
                     /locus_tag="ACHUOY_002748"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR0683487.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLERLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3028..3861
                     /locus_tag="ACHUOY_002749"
                     /pseudo
     CDS             3028..3861
                     /locus_tag="ACHUOY_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            3938..5020
                     /gene="lacI"
                     /locus_tag="ACHUOY_002750"
     CDS             3938..5020
                     /gene="lacI"
                     /locus_tag="ACHUOY_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR0683488.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            5143..8217
                     /gene="lacZ"
                     /locus_tag="ACHUOY_002751"
     CDS             5143..8217
                     /gene="lacZ"
                     /locus_tag="ACHUOY_002751"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR0683489.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPHWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            8269..>8533
                     /locus_tag="ACHUOY_002752"
     CDS             8269..>8533
                     /locus_tag="ACHUOY_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0683490.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         1941 a         2389 c         2334 g         1869 t
ORIGIN      
        1 aactatggcc gcccatcgag ttgcccagca ggtggatttt ggcgatatcc agttgatcca
       61 ccacgctttt caggattcgt gcattaagat ccgatcgtga accactatta acgatcgaat
      121 cgcttttgcc ccaacccgga caatccagca ggatcacccg atagcccgcc tctaccagcg
      181 gatcgatatt gcggctgaag ttcgcccagc cagtagcacc cgggccggaa ccatgcagca
      241 ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg gatgcgcagc gttttacccg
      301 cttcttctac attcagaaaa cggctggtgg cggcttcggt ttgtggctga taactcatca
      361 tcttctcctg catattcagt tctctgttct ggcgcttaac gagccaaatc cggcaatcca
      421 ctccgggatt gggcggtaat aacgcccttc gctacgccag ttgccaaatg cagaaatagc
      481 ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac tttccggcaa tggcggaaag
      541 ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc tgctccagca aagtcatgaa
      601 ctggttatcc caaatcgggt tgagcggatg cagagttctc tgatcctcaa caaacttctc
      661 agcggcgcta atcacccttt gctgacgcaa ttcgcgctca ctggcgggta aatctttccc
      721 gctccccaac agacggtcgc gcatatgggc atcggctttc gccagttcgg gcaccggcgg
      781 ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt ttattgagag tgctggtgaa
      841 acgtccaatg gcttcaccca acatgcgggt acgctggaaa ccgggcagcg gcgtggcgac
      901 accgttgatg aacacaggca gaactggcac cttatccagc ccaccgagca ggaactccag
      961 cggctgggcg aacccgtggt ccacctgcat acagtaagaa acggcaagat cgatcccgct
     1021 cttcatgaca gcatgcgcac aggcctccgc cagctccaca ggcacgggca gctctcctgc
     1081 cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct aaacagaacg gtggcatcac
     1141 gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc accaccagtt caggggagaa
     1201 agccgcaata cgctcgcggg cgctggcaat cacgccattg acctcatcga gtacctcttg
     1261 cgccgggtcg acatacccca ccaacggcga gtgggaaaga cagtgaagat aagcgtgcat
     1321 atcaggctac cttttcgaca gaaacgtcgg catcagggcg ggtcagcgtc atcaccgacg
     1381 ccagtttatt cagcgtattg cccagggttt gcggaatggc ggtggcggca acaaagcgat
     1441 ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa ccagctacgc agacgacctt
     1501 gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg tgcggtatga atttgcactt
     1561 ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca ctgctggatt tgctcgtcgc
     1621 tcatccccca cagtggattg catccccagc caattaccgc gaagttcgcg ccgatcgcgt
     1681 tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt cggctgaata aacatcttgc
     1741 cgaccggaga gtgcttcgct tcgccctcac gcaccagcgc accgccgtaa tattgcggca
     1801 tcggcttgaa gcgcatttcg aggaagtagc gttttactgg cggcagataa ttcaacagcc
     1861 aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg cggagccagc acgttgcccg
     1921 ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg acgttcttgt tgataggtat
     1981 cgagcagcgc atcgcgggct ttcccctgga taaccaacgc cagtttccag gcgaggttaa
     2041 aggcgtcgcg cataccactg ttatagccct gcccctgcca caccggcatg atgtgcgcgg
     2101 catcgcccgc cagcagtacg cgatcaatac ggaaacgttg cgccagtcgc gcgttatggg
     2161 tgtagacacg ctggcgaatc aattcaacat tgtccggatt aggcagcact ttgcttaaca
     2221 gcttgcgcat attttgcggc tcacgcagct gctcttcggt ttctcccggc atcaccataa
     2281 attcaaagcg acgtaccgca tgaggcagcg cggcagaaac atacgggcgc accggatcgc
     2341 aacacaaata gatatgcggc gtacttaacg gatcgttggc gatatctacc acaatccact
     2401 gatttggcgc agttttacct tcaaacggca cattcagggt gcgacgaaca aaacttgccc
     2461 caccatcaca ggccaccagc cactgggctt tgatcgtttc ccgctgccct tctgccgttt
     2521 tcaggtgcaa ggtcacttcg tcatcttgct ggctgaaggc ctccagctcg cgggaaaaca
     2581 agcagcgcac attcggaaaa cgcgacaacc tttccagcat caccgcatcg acctgtggct
     2641 gaataaaggc gttacggcgc ggccagccaa attcatcggt cattggctga atatcagcaa
     2701 aacagcggcc ttttggggtg agaaaacgca tcgcgtgcca cggtgtagtg tgcggcagaa
     2761 cattctcgac caggccgacc gactgcatgg tgcgcagcgc ctcgtcatca ataccaatcg
     2821 cacgcgggta gtcgatcaac ttatcgagtt tctccaccac cagcacgtca atgcccatct
     2881 gaccgagata gttcgccatc atcagcccaa ccggaccggc accagcgatc gccacctgaa
     2941 cgctatggtt aacagcaggc tggatgtcag ggtgttgtat tgccatttct gcacaacgtt
     3001 gttttaacta tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc
     3061 gtgcgcggct taacccgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt
     3121 gccagcgtcg ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg
     3181 ctggagacgc tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt
     3241 ctgaccatca aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca
     3301 ctggcggccc cgctgctggg cgatctgttg cgcgaagtgg tatgaccaac agatgtgtcc
     3361 accctggatg ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc
     3421 tttcaccggg caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc
     3481 tggctggcct tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc
     3541 cccggtgatg actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc
     3601 gcgcgcaaag agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc
     3661 tctatcgccg taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat
     3721 atggcgagcg caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg
     3781 gtagcaaaac agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga
     3841 agcggcgtgc atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg
     3901 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
     3961 tgtcgcagag tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag
     4021 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     4081 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     4141 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     4201 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     4261 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     4321 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     4381 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     4441 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     4501 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     4561 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     4621 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     4681 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     4741 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     4801 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     4861 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     4921 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5101 gtgaaattgt gagcggataa caatttcaca caggaaacag ctatgatcat gattacggat
     5161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     5341 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     5401 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
     5461 cccattacgg tcaatccgcc ttttgttccc gcggagaatc cgacgggttg ttactcgctc
     5521 acatttaata ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggt
     5581 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagc
     5641 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggaaaaaa ccgcctcgcg
     5701 gtgatggtgc tgcgctggag tgacggtagt tatctggaag atcaggatat gtggcgaatg
     5761 agcggcattt tccgtgacgt ctcgttgctg cacaaaccga gcacacaaat cagcgatttc
     5821 catgttgcca ctcactttaa tgatgatttc agccgcgcgg tactggaggc agaagttcag
     5881 atgtacggcg agctgcgcga tgagctgcgg gtgacggttt ctttgtggca gggtgaaacg
     5941 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
     6001 gccgatcgcg tcacgctacg tctgaacgtc gaaaacccgg cgctgtggag cgccgaaatc
     6061 ccgaatctct atcgtgcggt ggttgaactg cacaccgacg acggcacgct gattgaagca
     6121 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     6181 ggcaagccgt tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag
     6241 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     6301 gccgtgcgtt gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgcttc
     6361 ggcttgtacg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     6421 ctgaccgatg atccgcactg gttacccgcg atgagcgaac gcgtaacgcg aatggtgcag
     6481 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgagtc aggccacggc
     6541 gctaatcacg acgcactcta tcgctggatc aaatctgtcg atccatcccg cccggtgcag
     6601 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     6661 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     6721 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     6781 cttggcggct tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
     6841 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaatgg caatccgtgg
     6901 tcggcttacg gcggtgattt tggcgataca ccgaacgatc gccagttctg catgaacggt
     6961 ctggtctttg ccgaccgcac gccgcatcca gcgctgacag aagcaaaaca ccagcagcag
     7021 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     7081 catagcgata acgagcttct gcactggtcg gtggcgttgg atggcaagcc gctggcaagc
     7141 ggtgaaatgc ctctggatgt tgctccacaa gataaacagt tgattgaact gcctgaacta
     7201 ccgcagccgg agagcgccgg acaactctgg cttacggtac acgtagtgca accgaacgcg
     7261 accgcatggt cagaagccgg acacatcagc gcctggcagc agtggcgtct ggcggaaaac
     7321 ctcagcgtga cactccccgc cgcgccccac gccatcccgc aactgaccac cagcgaaacg
     7381 gatttttgca tcgagctggg taataaacgt tggcaattta accgccagtc aggctttctt
     7441 tcacagatgt ggattggcgc tgaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     7501 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga cccgaacgcc
     7561 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     7621 acggcagata cacttgccga cgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     7681 ggaaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     7741 attaccgttg atgttgaagt agcgagcgat acaccgcatc cggctcggat tggcctgacc
     7801 tgccagctgg cgcaggtagc agaacgggta aactggctcg gattagggcc gcaagaaaac
     7861 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     7921 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     7981 tatggcccac accagtggcg cggcgacttc cagttcaata tcagtcgcta cagccaacaa
     8041 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     8101 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtttcagcg
     8161 gaattccagc ttagcgccgg tagctaccat taccagttgg tctggtgtca aaaataataa
     8221 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     8281 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatgggg
     8341 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     8401 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     8461 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
     8521 gtgatgtttg cgc
//
LOCUS       DAZOYL010000167         8532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZOYL010000167 DAZOYL010000000
VERSION     DAZOYL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.167"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            12..542
                     /locus_tag="ACHUOY_002753"
                     /pseudo
     CDS             12..542
                     /locus_tag="ACHUOY_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1112..1201)
                     /locus_tag="ACHUOY_002754"
     tRNA            complement(1112..1201)
                     /locus_tag="ACHUOY_002754"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1165..1167),aa:Ser,seq:cga)
     gene            1295..2092
                     /gene="mtfA"
                     /locus_tag="ACHUOY_002755"
     CDS             1295..2092
                     /gene="mtfA"
                     /locus_tag="ACHUOY_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR0683491.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2193..2268
                     /locus_tag="ACHUOY_002756"
     tRNA            2193..2268
                     /locus_tag="ACHUOY_002756"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2226..2228,aa:Asn,seq:gtt)
     gene            2582..8532
                     /gene="yeeJ"
                     /locus_tag="ACHUOY_002757"
                     /pseudo
     CDS             2582..8532
                     /gene="yeeJ"
                     /locus_tag="ACHUOY_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         2402 a         2077 c         2026 g         2027 t
ORIGIN      
        1 gagctacaaa aatgaaccga ttctcaaaaa ctcaaattta tttacattgg ataacgctgc
       61 ttttcgttgc aataacctat gccgcgatgg aactccgtgg ctggtttcct aaaggtagta
      121 gtacttatct gctgatgcga gaaacacatt acaatgcggg tatattcgtt tgggtgttaa
      181 tgttttcacg cctgattata aaacaccgtt atagtgatcc ttctattgta ccaccgccac
      241 ctgcctggca aatgaaagcg gcttcgctaa tgcacatcat gctttatata accttccttg
      301 cacttcctct gctggggatt gctttgatgg cttacagtgg aaaatcgtgg agtttccttg
      361 gtttcaatgt gtctcccttt gttaccccaa acagcgaaat taaagcactg ataaaaaata
      421 ttcacgaaac ctaggcaaat ataggttact ttttaatcgc agctcacgct ggcgcagcac
      481 tctttcatca ctacattcag aaagataata ctctgttacg aatgatgcat cgccgcaaat
      541 aagtcgatcc cctgacaagg aaatgtgaac catgtacatc aacgcgtcat aaatgtttac
      601 gcaagtgcac aatgcatcct ttctactatc aacaaggagg agcagaaact gtgtaaatcg
      661 tacaatggta agacttatca aaaatacagg ttaattttgg tatttaaacc ttttttactt
      721 gttagtgtga tacaaaggct ttcaaaaaag ctgggaaatt caacaactct tggaaattat
      781 ggactttgtc ccgcggcttt aatctgacgg ccgcgttcct tttttgactc aattattgac
      841 cccttctcta cgcaacttca gttcccacca ccaactttgc ggcagtttca taggatgaat
      901 gtctaaaaga ataatggtga gcgataagaa aacgactgaa taactgcaca ttttcgctcg
      961 aaagcttcct gtcagatcca tagcgaatca agtgctgaat gtcacagtat cgaacagaaa
     1021 acagtgacga tctaaccctt caagaatatt ctacaattgt tctgtttagg aaaagcaagg
     1081 cgggaagtca ggagataagt cattgataaa gtggcggaga gagggggatt tgaacccccg
     1141 gtagagttgc ccctactccg gttttcgaga ccggtccgtt cagccgctcc ggcatctctc
     1201 cgttcagatg gttgccatga tgccaggaaa tttggcattt taacagtccc tgttcgtgca
     1261 attttgttca agtgacgagt ttgcgagcaa aacgatgatt aagtggccct ggaaagtaca
     1321 agaatcagca catcaaactg cccttccctg gcaggaagca ctatcgatcc cccttttaac
     1381 gggtctgacg gaacaggaac aaagcaaatt agtcactctt gccgaacgtt ttttacagca
     1441 aaagcggctt gttcctttac agggctttga actggattca ttaagaagct gccggatagc
     1501 acttctattt tgcctacccg ttctggagtt aggactggaa tggctggatg gttttcatga
     1561 agtcttaatt tatcctgcgc catttgtggt cgatgatgaa tgggaagacg atatcggtct
     1621 ggtgcataac caacgtattg ttcagtcagg tcagagctgg cagcaagggc ctatcgtttt
     1681 gaactggttg gatatacaag attcttttga tgcatctggt tttaacctga ttattcatga
     1741 agtcgctcat aagctggaca cccgtaacgg cgatcgcgcc agcggagttc cctttattcc
     1801 gttgcgtgag gttgctggct gggaacacga tcttcatgct gcaatgaaca acattcagga
     1861 agaaatcgaa ttggttggcg agaatgcggc gagcattgat gcttatgcag ccagcgatcc
     1921 tgctgaatgt tttgccgtac tttctgaata tttctttagc gccccagaac tttttgctcc
     1981 tcgtttccct tcattgtggc aacgtttctg ccaattttat caacaagatc ctttgcagag
     2041 actgcatcac gctaatgata cagactcgtt ttcggcgacg aatgttcatt aattaacaac
     2101 tttgcagatt aattaaccaa ttgaaatgac ttatgaaatt tagtgttgac agacaaggta
     2161 ccgctaagta atatgtgccc cgttcacacg attcctctgt agttcagtcg gtagaacggc
     2221 ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa ttctaaaaat
     2281 tcgctttttt agcgcaatgt cactgacctt agttgaacat tgttttttaa cggatagcgg
     2341 gtttttaaca ccttaagcgc cctcgacctt tatggttgag ggcgttttgc tatgaacgcc
     2401 atcaccattt tcccctcgat tataaaactt gagttattca gtagtctccc ctcttgcaac
     2461 tcacacccaa aactgcctaa cgaaaagtta ttaattttca atcgtattgc tatcagtatt
     2521 tacatttttt cgctgtgcta gaaagggcgc atttatgtta gctcgttcag ggaaggtaag
     2581 catggctacg aagaagagaa gtggagaaga aataaatgac cgacaaatat tatgcgggat
     2641 gggaattaaa ctacgccgct taactgcggg tatctgtctg ataactcaac ttgcgttccc
     2701 tatggctgcg gcagcacaag gtgtggtaaa cgccgcaacc caacaaccag ttcctgcaca
     2761 aattgccatt gcaaatgcca atacggtgcc ctacaccctt ggagcgctgg aatcggccca
     2821 aagcgttgcc gaacgtttcg gtatttcggt ggctgagtta cgcaaactca accagtttcg
     2881 tacgtttgct cgaggttttg ataatgtccg ccagggtgat gaactggatg tcccggcaca
     2941 agttagtgaa aaaaaattaa ccccgccgcc gggtaatagc agtgacaacc tcgagcaaca
     3001 gatagccagt acttcacagc aaatcgggtc tctgctcgcc gaagatatga acagcgagca
     3061 agcggcaaat atggcgcgtg gatgggcctc ttctcaggct tcaggcgcaa tgacagactg
     3121 gttaagccgc ttcggtaccg caagaatcac gctgggcgtg gatgaagatt ttagcctgaa
     3181 gaactcccag ttcgattttc tccatccgtg gtatgaaacg cctgataatc tctttttcag
     3241 tcagcatact ctccatcgta ctgacgagcg tacgcagatt aacaacggct taggttggcg
     3301 tcatttcact cccacatgga tgtcgggcat caacttcttt ttcgaccacg atcttagccg
     3361 ttaccactcc cgcgtcggca ttggcgcgga gtactggcgc gactatctaa aattaagcag
     3421 taacggctat ttgcgactga ccaactggcg cagcgcacct gaactggaca acgattataa
     3481 agcacgcccg gccaatggct gggatgtacg cgcagaaagc tggctacccg cctggccgca
     3541 ccttggcggt aaactggtct atgaacagta ttatggcgat taagtggccc tgttcgataa
     3601 agacgatcgg caaagtaatc ctcatgccat aaccgctgga cttaactata cccccttccc
     3661 gctgatgacc ttcagcgcgg agcaacgcca gggtaaacag ggcgaaaatg acacccgttt
     3721 tgccgtcgat tttacctggc aacctggcag cgcaatgcag aaacagcttg acccgaatga
     3781 agtcgctgca cggcgtagcc ttgcaggcag ccgttatgat ctggtggatc gcaacaacaa
     3841 tatcgttctg gaatatcgca aaaaagaact ggttcgccta accctgacag accccgtgac
     3901 agggaagtca ggagaagtga aatcactggt ttcgtcgcta caaaccaaat atgccctgaa
     3961 aggctataac gtcgaagcca ccgcactgga agctgccggt ggcaaagtgg tcacaacggg
     4021 taaagatatt ctagttaccc tgccggctta ccggttcacc agtacgccag aaaccgataa
     4081 cacctggccg attgaagtca ccgccgaaga tgtcaaaggc aatttgtcga atcgtgaaca
     4141 gagcatggtg gtcgttcagg cacctacgct aagccagaaa gattcctcgg tatcgttaag
     4201 tacccaaaca ttgaacgcgg attcccattc aaccgccaca ctgactttta ttgcgcatga
     4261 tgcagcaggt aatcctgttg tcgggctggt gctctcgacg cgtcacgaag gtgttcagga
     4321 catcaccctt tctgactgga aagataatgg tgacggaagc tatacccaga tcctgaccac
     4381 aggagcgatg tctggcacgc tgacgctgat gccacagctg aatggtgtgg atgcggctaa
     4441 agcccccgcc gtggtgaata tcatttctgt ttcgtcatcc cgaactcact cgtcaattaa
     4501 gattgataag gaccgttatc tctccggcaa tcctatcgag gtgacggtag aactgagaga
     4561 tgaaaatgac aaacctgtta aggaacaaaa acagcaactg aataacgcag tcagcatcga
     4621 caacgtgaaa ccaggagtca ctacagactg gaaagaaacc gcagatggcg tctataaggc
     4681 gacctatacc gcctatacca aaggcagtgg acttactgcg aagctattgg aatgaagatt
     4741 tgcataccgc tggatttatc atcgacgcca acccgcagtc agcgaaaatt gcgacattat
     4801 ctgccagcaa taatggtgta ctcgccaatg agaatgcagc aaacaccgtc tcggtcaatg
     4861 tcgctgatga aggaagcaac ccaatcaatg atcataccgt cacgtttgcg gtattaagcg
     4921 gatcggcaac ttccttcaac aatcaaaaca ccgcaaaaac ggatgttaat ggtctggcga
     4981 cttttgatct gaaaagtagt aagcaggaag acaacacggt tgaagtcacc cttgaaaatg
     5041 gcgtgaaaca aacgttaatc gtcagttttg tcggcgactc gagtaccgcg caggttgatc
     5101 tgcagaagtc gaaaaatgaa gtggttgctg acggcaatga cagcgccaca atgaccgcga
     5161 ccgtccggga tgcaaaaggc aacctgctca atgacgtcat ggtcactttc aatgttaatt
     5221 cagcagaggc gaaactgagc caaaccgaag tgaatagcca cgacgggatc gccacagcta
     5281 cgctgaccag tttgaaaaat ggtgattata gggttacggc ctctgtgagc tctggttccc
     5341 aggctaatca acaggtgaat tttatcggtg atcaaagtac tgctgccctg accctcagtg
     5401 tgccttcagg tgatatcacc gtcaccaaca cagctccgca acatatgact gcaaccttgc
     5461 aggataaaaa tggcaaccca ctaaaagata aagaaatcac cttctctgtg ccaaacgacg
     5521 tcgcaagtaa gttctcgatt agcaacggag gaaaaggcat gacggatagt aacggggttg
     5581 caatcgcctc cctgaccggc acgttagcgg gcacgcatat gatcatggct cgtctggcta
     5641 acagcaatgt cagcgatgca cagccaatga cgtttgtggc ggataaagac agagcggttg
     5701 tcgttttgca aacatcgaaa gcggaaatca ttgggaatgg cgtggatgag acaactctga
     5761 cagcaacagt gaaagatccg tcgaatcatc cggtggcggg gataacggta aacttcacca
     5821 tgccacagga cgttgcggca aactttaccc ttgaaaataa cggtattgcc atcactcagg
     5881 ccaatgggga agcgcatgtc acgctgaaag gtaaaaaagc gggcacgcat acggttaccg
     5941 caacgctggg taataacaat accagtgatt cgcagccggt aacatttgtg gcggacaaag
     6001 cctcggctca ggttgtcctg cagatatcaa aagatgagat cacaggtaat ggcgtcgata
     6061 gcgcaacgct aactgcaacg gttaaagatc agttcgacaa tgaggtgaat aatcttccgg
     6121 taacattcag ctcagcctct tcaggactca ccctgacccc gggagtaagt aataccaatg
     6181 agtctggcat cgcgcaggcc actctcgcag gcgttgcctt tggtgagcag acggtcactg
     6241 catcactggc taataatggt gccagcgaca acaaaactgt gcattttatt ggcgacacag
     6301 cggcggcaaa aattatcgag ttgacgcctg tcccagacag cataatcgcc ggtaccccgc
     6361 agaacagctc cggcagcgcc atcaccgcca cagtcgttga taataatggc tttccggtga
     6421 aaggtgtgac tgtgaacttc accagcagaa caaactctgc cgaaatgacg aatggcggcc
     6481 aagccgtaac gaacgaacag ggtaaggcta ccgtcactta taccaatacc cgctcctcga
     6541 tagaatcagg agcgagaccg gataccgttg aggccagtct ggaaaatggt agctccacgc
     6601 ttagcacatc aattaatgtc aacgctgatg cgtctacggc acatctcacc tggctacagg
     6661 cactttttga tacagtctcc gcaggcgaga caaccagtct gtatattgag gtgaaggata
     6721 attacggcaa cggtgtcccc cagcatcagg tgaccctcag cgtttcacca agtgaaggcg
     6781 tgacccccag taataacgct atatatacta ccaaccacga cggcaatttt tacgcaagct
     6841 ttaccgctac aaaagccggg gtttatcaag tgacggcaac cctcgaaaat ggcgattcga
     6901 tgcaacaaac agtgacctat gtgccgaacg tcacgaatgc cgaaatcacg ctggcaacct
     6961 cgaaggatcc ggtgattgcc gacaataacg atctcacgac actaacagca acagtcgctg
     7021 atacagaggg caatgcgata gccaacactg aagtaacatt tactctgccg gaagatgtta
     7081 aggcgaactt cacgctgagc gatggcggta aagcgattac tgatgctgaa ggcaaagcga
     7141 aagtcacgct gaaaggtaca aaagcaggcg ctcatactgt tacagcatcg ataactggcg
     7201 gtaagagtga gcagttggtg gtgaacttta ctgcggatac gctcactgcg caggttaatc
     7261 ttaacgttac cgaggacaat tttatcgcta ataacgtcgg gatgaccagg ctgcaggcaa
     7321 cagtgactga tggaaacggc aacccgttag ccaatgaggc ggtgacattc acgctaccgg
     7381 cagatgtgag cgcaagcttt actctcggac aaggcggttc cgccattact gacatcaacg
     7441 gcaaggctga agttacactg agcggtacaa aatccggcac ctaccccgtg acagttagcg
     7501 tgaacaatta tggtgtcagt gatacgaaac aggtgacttt gattgccgat gctggtaccg
     7561 caacactagc ctccttaacc tctgtatact cattcgtcgt cagcacgacc gaaggcgcga
     7621 ccatgactgc aagcgtcact gacgctaacg gcaacccggt agaaggcata aaagttaatt
     7681 tccgcggaac ctccgtcacg ctaagcagca ccagcgttga aacggatgat cggggtttca
     7741 ctgaaattct tgtgacaagc accgaagtcg gactgaaaac agtttcagcc tctctggcag
     7801 ataaacctac tgaagtcata tcgcgattac tgaatgcaaa agcagatatt aattctgcaa
     7861 cgattaccag tctggagata cctgaaggtc aggtcatggt cgcacaagac gtagcagtta
     7921 aagctcacgt caacgaccag tttggcaacc cgattcttaa tgaatctgta acattcagtg
     7981 cagagccacc agagcacatg accatcagcc aaaatattgt ctctactgat acgcatggta
     8041 tagccgaggt ctccatgacg cccgaaagaa acggttcgta tatggtgaaa gcatccctgg
     8101 cgaatggagc ctcacttgag aaacaactgg aggctattga tgaaaaactg acactctcgg
     8161 cgtccagccc gcttatcggt gtcaattccc caacaggtgc aactctgacg gcaacgctaa
     8221 cttctgcaaa tggcactcca gtggagggtc aggtcatcaa ctttagcgta acgccagaag
     8281 gtgcgacgtt aagtggcgga aaagtgagaa ctaactcttc tggtcaggct ccagtcgttc
     8341 tgaccagcaa taaagtcggt acatatacgg tgactgcatc gttccataac ggcgtaacaa
     8401 tacagacaca gacaaccgtg aaagtcactg gcaactcaag caccgcccat gttactagct
     8461 ttatcgctga tccatcgact atcgccgcca ccaacagtga tttaagtacc ttaaaggcaa
     8521 cggttgagga tg
//
LOCUS       DAZOYL010000168         8522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZOYL010000168 DAZOYL010000000
VERSION     DAZOYL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.168"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            135..1457
                     /gene="dcuC"
                     /locus_tag="ACHUOY_002758"
     CDS             135..1457
                     /gene="dcuC"
                     /locus_tag="ACHUOY_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0683492.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(1500..1997)
                     /gene="sspB"
                     /locus_tag="ACHUOY_002759"
     CDS             complement(1500..1997)
                     /gene="sspB"
                     /locus_tag="ACHUOY_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR0683493.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2003..2641)
                     /gene="sspA"
                     /locus_tag="ACHUOY_002760"
     CDS             complement(2003..2641)
                     /gene="sspA"
                     /locus_tag="ACHUOY_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR0683494.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMCLGRS"
     gene            complement(3036..3428)
                     /gene="rpsI"
                     /locus_tag="ACHUOY_002761"
     CDS             complement(3036..3428)
                     /gene="rpsI"
                     /locus_tag="ACHUOY_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR0683495.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3444..3872)
                     /gene="rplM"
                     /locus_tag="ACHUOY_002762"
     CDS             complement(3444..3872)
                     /gene="rplM"
                     /locus_tag="ACHUOY_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR0683496.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4091..5218)
                     /gene="zapE"
                     /locus_tag="ACHUOY_002763"
     CDS             complement(4091..5218)
                     /gene="zapE"
                     /locus_tag="ACHUOY_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR0683497.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5412..5810
                     /gene="zapG"
                     /locus_tag="ACHUOY_002764"
     CDS             5412..5810
                     /gene="zapG"
                     /locus_tag="ACHUOY_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR0683498.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5964..7331
                     /gene="degQ"
                     /locus_tag="ACHUOY_002765"
     CDS             5964..7331
                     /gene="degQ"
                     /locus_tag="ACHUOY_002765"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR0683499.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            7421..8488
                     /gene="degS"
                     /locus_tag="ACHUOY_002766"
     CDS             7421..8488
                     /gene="degS"
                     /locus_tag="ACHUOY_002766"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR0683500.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT         2065 a         2160 c         2185 g         2112 t
ORIGIN      
        1 aatgaaaaat cacatcacct acaattaaaa acacgacatc cgcaccataa atagacttgc
       61 aaaaaatata acatcattgt tttcaatctg ccgtttatgg gattgaccgt tttcttttga
      121 cacggagttc aacaatgttc ggcataatta tatctgtcat cgtattaatt acgatgggct
      181 atttgatcct gaaaaactac aaacctcagg tggtgctggc tgccgcaggt gtcttcctga
      241 tgatgtgcgg agtctggtta gggttcggtg gtgtactcga tcccgccaaa agcagcggct
      301 acttgatcgt cgacatttat aatgaaatcc tgcgcatgct gtccaaccgc attgccggat
      361 tggggctgtc gattatggcg gtgggcggtt atgcccgcta catggagcgc ataggagcca
      421 gtcgcgcgat ggtgagcttg ctaagccgcc cgttaaaact cattcgctcg ccgtatatta
      481 ttctgtcggc aacttacgtc atcggccaaa tcatggcgca gtttattacc agcgcctccg
      541 gtctgggtat gttgctgatg gtcaccttat ttccgacgct ggtgagtctg ggagtaagtc
      601 gcctctctgc ggtggcggtt atcgcaacca cgatgtccat tgagtggggg attctggaaa
      661 cgaactccat ttttgcagcc caggtcgcgg gaatgaaaat tgccacatac ttcttccact
      721 accagcttcc ggtcgcctct tgcgtcatta tctcggtggc gatctcccac tttttcgtgc
      781 aacgcgcttt tgacaaaaaa gataaaaata tcaatcacga acaggcagag caaaaaactc
      841 tcgataatgt cccgccgctc tattacgcca ttttacctgt gatgccgtta atcctgatgc
      901 tcggctcgct gttcctcgcc cacgtcgggc tgatgaagtc agaactgcat ctggtggtgg
      961 tgatgttact aagtttgacg gtgacgatgt ttgttgagtt cttccgcaag cataacttgc
     1021 gcgaaacaat ggacgatgtg caggcgtttt ttgacggcat gggtacgcag tttgccaacg
     1081 tggtaacgct ggtggtcgcg ggtgaaatat ttgcgaaagg cttaacgacg attggcactg
     1141 ttgatgcggt tatcaggggt gcggagcatt ctggtctggg cggtattggc gtgatgatta
     1201 ttatggcgct agtcattgcc atttgtgcca ttgtgatggg ctctggcaat gcgccgttta
     1261 tgtcatttgc cagtcttatt ccgaatatcg cagccggact acatgtacca gcggttgtaa
     1321 tgattatgcc gatgcatttt gccacgacgc tagcgcgcgc ggtttcgccg attactgcgg
     1381 tggtgaagcg gacagcgatc cccatggcag tcggtttcgt ggtgaatatg attgccacaa
     1441 tcacgctatt ttattaagtc attaaaaaga caaaacaggc cgcctgggcc tgttttgtat
     1501 tacttcacaa cacgtaatgc cggtcgacca ccgcgtggtg gctgcggagg ttcatcgtca
     1561 ggatgagtgt catcatcgtg atctggcttg tcgccatcaa taaccgacat aacggtttcg
     1621 ttgtctgccg atgcctcttc atcattcatg atgctggtat cttcatcgta ggcagcttca
     1681 ggctcaaaca tcgtgcctgc gccattttca cgggcgtaga tagccagcac ggcagccagc
     1741 ggcacagaaa cctgacgcgg aatgccacca aagcgcgcgt taaagcgcac ctcatcattc
     1801 gccagttcca gattgccgac agcacgcggc gcaatgttga gtacgatttg cccgtcacgc
     1861 gcatattcca taggaacctg cacgccaggg agcgtcacat ccaccaccag gtgcggcgtg
     1921 agctggttat ccagcaacca ctcatagaat gcacgcagca gatagggacg acgtggtgtt
     1981 agctgtgaca aatccataca gattaactcc ggcccagaca catttcacgt tctgcttcag
     2041 ttaaagaagc aaggaaagag tcacgctcaa agacgcgggt catatagcct ttcagctctt
     2101 tcgcacccgg gccgctgagc tcgataccca gttgcggcag acgccacagc agcggagcaa
     2161 gatagcaatc gaccaggctg aactcatcgc tcaggaagta cggcttctga ccgaagaccg
     2221 gcgcaatcgc cagcagttct tcgcgcagtt gcttacgtgc ggcatctgct tcagaagctg
     2281 aaccgttgat gatggtgttc atcagcgtgt accagtcttt ttcgatgcga tgcatgtaca
     2341 gacggctttc accgcgagct accgggtaaa caggcatcag tggcggatgc gggaaacgct
     2401 catccagata ttccataatg atgcgagatt cccacagggt cagctcacga tccaccaggg
     2461 tcggaacgct ctgattcggg ttgaggtcaa tcagatcctg aggcggattg tccttttcca
     2521 cgtgttcgat ctcgaaactt acacctttct cagccagcac aatgcggacc tgatggctat
     2581 agatgtcagt aggaccggaa aacagcgtca ttaccgaacg tttgttggca gcgacagcca
     2641 tgaaaacctc caggtatagt cagaattttt actgctacca gccaccaggt ggccagtcag
     2701 aagttgtgtt acccaataag gaacgactct ctttgttcga aaatcaaaca aaaaatgagc
     2761 aatacccgac atttgggcag aaaattggat gatagtttac cagattttgc gaccattgtg
     2821 gtgagtggat gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat agttgactta
     2881 gacccttatt ggcgatgtgg tttttgtttt gcctgtcttt cagacggcag caaaaagcaa
     2941 cttcccattt tttacgctga ttcagaattt agccataaaa aaacccgccg aagcgggttt
     3001 tttcgtaaat tgttttctgc cggagcagaa gccaattaac gtttggagaa ctgcggacga
     3061 cgacgtgctt tacgcagacc gactttctta cgttcaacct gacgagcgtc acgagtaacg
     3121 aagccagctt tacgcagttc agaacgcagg gactcgtcgt attccatcag agcgcgggtg
     3181 ataccgtgac ggatcgcacc agcctgacca gagataccac cacctttaac ggtgatgtac
     3241 aggtccagtt tctcaaccat gtcgaccagt tccagcggct gacgaactac catgcgggca
     3301 gtttcacgac cgaagtactg ttccagagaa cgttggttga ttacgatttt accgttgccc
     3361 ggtttgatga aaacgcgagc tgcggaattt ttgcggcgac cagtgccgta gtattgattt
     3421 tcagccattg cctataatcc cgattagatg tcaagaactt gcggttgctg tgccgcgtgg
     3481 ttgtgctcgt tacccgcgta aactttcagt ttacggaaca tagcacgacc cagcgggcct
     3541 tttggcaaca tgcctttaac cgcgatttca atcacacgct caggacggcg agcaatcatc
     3601 tcttcaaagc tcacttgttt gataccaccg atgtggccgg tgtggtgata gtacactttg
     3661 tcagtacgct tgttgccggt tacagcaact ttgtcagcgt tcagaacgat gatgtaatca
     3721 ccggtatcta cgtgcggagt gtattccgct ttgtgcttac cgcgtaggcg acgagccagt
     3781 tcagtagcca gacggcccag agttttaccg gtcgcgtcaa caacatacca gtcgcgtttt
     3841 acggtttctg gtttagctgt aaaagttttc attaaaagct tacccaataa atagttacac
     3901 gttggtgaac acccaaacgt cttcaattgt tgaggttcac acgacaaagt ccggcaaacc
     3961 taccccttcg aatagcctat gccagcacac aaaaagtttt gggaaaaaaa ctttcttgta
     4021 acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt tttgtgattt
     4081 gtgacaggtt ttaacccgcc aaatgctcgc gcttcagata ctcttcgctt tgcatctctt
     4141 gcagacgtga caggcaacgc tggaactcaa acttcagccg atcgccctga taaatttcat
     4201 acagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac tcatccacca
     4261 gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt cataactggt acatcaaaca
     4321 acatgaccgt atgaaagaga cgtgagagcg caatatagtc atgctgactg cgggcgtcga
     4381 cgcacagcgt agtaaaagag accgccagcg tctggttctc gacgcccatt gtcgctaatg
     4441 gccgatggtt gatttctaac gtcggtgaat tttctcgttt cccccccgcc agcgccaacc
     4501 atagtttatc catttgcgcc cgggtttcat cgtggagtgg cgaaagccac agatgcgcct
     4561 gagtgagtgt acgcagtcga taatcaacac cagcgtccac gttcattaca tcacaatgct
     4621 gtttaatggc atcgattgca ggcagaaaac gcgcacgttg caagccattt cgataaagtt
     4681 cgtccggcgg aatatttgac gtcgctacca gggtaatacc gcgggcgaac aaggctttca
     4741 tcagaccgcc aagtagcatg gcatcggtaa tatcagaaac aaaaaattcg tcaaaacaga
     4801 gcacgtcagt ttcggcttta aagcgatcgg caataatttc cagcggatcg gtctgcccct
     4861 gtaaggcagt tagctcttca tgcacccgca gcataaaacg gtgaaagtgc aggcgctgtt
     4921 tccgctctcc cggcaggctt tgatagaaaa ggtccatcag ccaggttttc ccgcgtccta
     4981 caccgcccca catatataag ccacgcactg gcgtatgctt tgtgtcttcg cgtttacccc
     5041 acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggtggc gtgctattga
     5101 tgagttcctg ataaataatt tccaggcggc tgacggcctc tttttgaacg tcgtcgggtt
     5161 gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggggtaacg ctttgcatga
     5221 tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg aaaaaaaggc
     5281 cgttctacac tacgcgatat gcagtcggga ttccacttct gtggaattaa cggttatagt
     5341 ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac aaaagacaac
     5401 gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc atcattattg
     5461 gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg ttgcagtacg
     5521 aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt agccactttg
     5581 cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg tatcagcaca
     5641 tggcgaaaag ttccagcagc ctgctgccgg aactgtctgc tgaagcaaac ccgttccgta
     5701 atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcagatg cctcgcgact
     5761 attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa tttatttttc
     5821 gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta atctggtgtc
     5881 tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc ttttcttatc
     5941 attcaggtac gagagcagga ataatgaaaa aacaacccca gctgttgagt gcattagcgt
     6001 taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt ccaggccagg
     6061 ttgccgatca ggcccctctc cccagtctgg ctccaatgct ggaaaaagtg cttccggcag
     6121 tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg gaagaattca
     6181 aaaagttttt tggtgatgat ttaccggagc aacctgcaca acccttcgaa ggtttaggct
     6241 ccggtgtcat catcaacgcc agtaaaggct atgtgctgac caacaaccat gtgattaatc
     6301 aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca aaactgattg
     6361 gtagcgatga ccagagcgat atcgccctgt tacaaattca aaacccgagc aaattaacgc
     6421 aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgta gcggtcggta
     6481 acccatttgg ccttgggcaa accgccacct ctggcattgt ttccgcatta ggacgcagcg
     6541 ggttgaatct tgaaggtctg gaaaacttta tccagacaga tgcttccatt aaccgcggta
     6601 actccggcgg tgcactgtta aaccttaacg gtgagttaat tggcatcaac actgcaatcc
     6661 ttgcgcctgg cggcgggagc gtcgggattg gatttgccat ccccagtaat atggcgcgaa
     6721 cactggcgca gcagcttatc gactttggtg aaatcaaacg cggtttgtta ggcatcaaag
     6781 gcaccgagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag cgtggcgcgt
     6841 ttgtcagcga agtgttgcca ggttctggct cggcgaaagc gggcgtcaaa gcgggcgata
     6901 ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc tctcgtatcg
     6961 cgaccaccga gccgggcaca aaagtgaaac ttggcctgct gcgtaacggc aaaccactgg
     7021 aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa atgatcgcgc
     7081 cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc aaaggtatta
     7141 agatcgatga agttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa aaagacgatg
     7201 tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt aaagtgctgg
     7261 cggcaaaatc ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc atctatctgc
     7321 tgatgcgtta atgtcgtaaa ccgggcatca ggcttacgtg tgatgtccgg ttaactcgtg
     7381 gtatgctgct gccgttccct tttttaatga cgcctccatc atgtttgtga agctcttacg
     7441 ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc cttcgctgcg
     7501 cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgagacgc ctgccagcta
     7561 taatctggcg gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc gtggtttgaa
     7621 caccaactct cacaaccagc ttgagatccg caccctggga tccggtgtaa tcatggatca
     7681 acgcggttat atcatcacca ataaacacgt catcaacgac gccgatcaga tcatcgtcgc
     7741 cttacaggat ggtcgtgtat ttgaagcatt gctggtggga tctgactctc taaccgatct
     7801 ggcggtactt aaaattaatg ccactggcgg tttacctacc attccaatca atgcacgtcg
     7861 cgtaccgcat attggcgacg tggtactggc gatcggtaac ccgtacaacc tcgggcagac
     7921 cattacccag gggattatta gtgccacggg tcgaattggt ctgaacccga ccgggcggca
     7981 aaacttcctg caaaccgatg cttccattaa ccacggtaac tctggcggcg cgctggtgaa
     8041 ctcgctgggc gaactgatgg gcattaacac gctgtcgttt gataagagta acgatggcga
     8101 aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa ttatggataa
     8161 gctgatccgc gatggtcgcg tgatccgcgg ctacattggc attggcggac gtgagatcgc
     8221 accgctgcac gcacagggcg gtggtataga tcaactgcaa gggatcgtgg ttaatgaagt
     8281 gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc aacgatctga ttatttcggt
     8341 ggataacaaa ccggccatct ctgctctgga gacgatggat caggtggcgg aaattcgccc
     8401 tggttcggtg atcccggttg tagtgatgcg tgatgataag cagttaacgc tgcaggtcac
     8461 cattcaggaa tatccggcaa ccaattaagt cgagtgctca aaacaaaaaa ccggagtctg
     8521 tg
//
LOCUS       DAZOYL010000169         8510 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZOYL010000169 DAZOYL010000000
VERSION     DAZOYL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.169"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            370..1347
                     /gene="glaH"
                     /locus_tag="ACHUOY_002767"
     CDS             370..1347
                     /gene="glaH"
                     /locus_tag="ACHUOY_002767"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR0683501.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1367..2635
                     /gene="lhgO"
                     /locus_tag="ACHUOY_002768"
     CDS             1367..2635
                     /gene="lhgO"
                     /locus_tag="ACHUOY_002768"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR0683502.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2658..4106
                     /gene="gabD"
                     /locus_tag="ACHUOY_002769"
     CDS             2658..4106
                     /gene="gabD"
                     /locus_tag="ACHUOY_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0683503.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4120..5400
                     /gene="gabT"
                     /locus_tag="ACHUOY_002770"
     CDS             4120..5400
                     /gene="gabT"
                     /locus_tag="ACHUOY_002770"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR0683504.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5711..7111
                     /gene="gabP"
                     /locus_tag="ACHUOY_002771"
     CDS             5711..7111
                     /gene="gabP"
                     /locus_tag="ACHUOY_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR0683505.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7132..7794
                     /gene="csiR"
                     /locus_tag="ACHUOY_002772"
     CDS             7132..7794
                     /gene="csiR"
                     /locus_tag="ACHUOY_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR0683506.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7795..8244)
                     /gene="kbp"
                     /locus_tag="ACHUOY_002773"
     CDS             complement(7795..8244)
                     /gene="kbp"
                     /locus_tag="ACHUOY_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR0683507.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8328..8486)
                     /locus_tag="ACHUOY_002774"
     CDS             complement(8328..8486)
                     /locus_tag="ACHUOY_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR0683508.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1926 a         2222 c         2368 g         1994 t
ORIGIN      
        1 aacaggagaa ccgccgaact atctctattt ataatttaat gttatatctg ccccgataaa
       61 acggggcaga taatatgttt agtttactaa cggtcatttt gcagtgaagc catttactgt
      121 tttttatcga ccagataatc tgttctctaa tgttaactcc ccctaacctg ttgctttagt
      181 tattcatttc ctgtctcact ttgccttaat actctacgtt aaatgttact aatttgttgc
      241 ttttgatcac aataagaaaa caatatgtcg cttttgtgcg catttttcag aaatgtagat
      301 atttttagat tatggctacg aaatgagcat cgccatgtca ccctacatct cataagagga
      361 tcgcttctga tgaatgcact gaccgccgta caaaataacg ctgtcgattc aggccaggac
      421 tatagcggat tcactctcat cccgtcggcg caatccccgc gtctgctgga actcaccttc
      481 accgaacaga cgaccaaaca gtttctcgag caggttgccg aatggcccgt acaggcgctg
      541 gagtacaaat cgtttctgcg ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaat
      601 cagctgcaac cattgctgtt gaagaccctg ctaaaccgtg ctgaaggtgc gctgttgatc
      661 aatgcggtgg gtatcgatga tgtggcgcag gcagatgaga tggtgaagct ggcaacggcg
      721 gtagcgcatc tgattggtcg ctcaaatttc gatgcgatga gcggtcagta ttacgcgcgt
      781 ttcgtggtga aaaatgtcga taactcagac agctatctgc gtcagccgca ccgcgtgatg
      841 gaactgcaca acgacggcac gtatgtcgaa gagatcaccg attacgtgct gatgatgaaa
      901 atcgatgagc aaaacatgca gggtggaaat tcgttgctgc tgcatctcga tgactgggaa
      961 catctggacc actatttccg ccatccactg gcgcgtcgcc cgatgcgctt tgccgcgccg
     1021 ccgagcaaaa acgtcagcaa agatgttttc catccagtgt tcgacgttga tcaacagggt
     1081 cgcccggtga tgcgctatat cgaccagttc gtccagccaa aagattttga agaaggcgtg
     1141 tggttgagcg agctttccga tgccattgaa accagcaaag gcattctttc tgtacccgtt
     1201 cccgttggca aattcctgtt gataaacaac ctgttctggc tgcacggtcg cgaccgcttt
     1261 actccgcacc cggatctgcg ccgtgaactg atgcgtcagc gtggctattt cgcttacgcc
     1321 actcaccact accagacgca tcagtaagcg caaaggaatt gagcggatgt atgattttgt
     1381 gattattggc ggcggcatca tcggcatgtc gaccgccatg caactgattg atgtttatcc
     1441 ggatgcccgc attgcgttgc tggaaaaaga gtccggcccg gcctgtcacc agacgggcca
     1501 caacagcggc gtgatccatg ccggggtcta ttacacgccc ggcagcctga aggcgcagtt
     1561 ttgcctggcg gggaaccgcg ccaccaaagc cttttgcgat caaaacggca ttcgctatga
     1621 caactgcggc aagatgctgg tcgccacgtc tcagctcgaa atggaacgga tgcgcgcctt
     1681 atgggaacgc acagcggcga acggtatcga gcgcgagtgg ataaacgccg tggaactgcg
     1741 cgagcgcgaa ccgaatatca ccgggcttgg tggcattttt gtgccgtcca gcggcattgt
     1801 cagctaccgc gaagtcacag cggcaatggc aaaaatcttc caggccagag gcggcgagat
     1861 tatttataac gccgaagtca gcgccctcag taagcataaa aacggcgtgg tgatacgcac
     1921 ccgtcagggc ggtgaatatg aagcatcgac gctgattagc tgttccgggc tgatggctga
     1981 ccggctggtg aaaatgctcg gcctcgaacc gggctttatc atctgcccgt tccgtggtga
     2041 gtatttccgc cttgcgccgg agcataacca gattgttaac cacctgattt accccattcc
     2101 cgaccccgcg atgccatttt tgggcgtgca tctcacccgc atgatcgacg gcagcgtgac
     2161 cgtcgggcca aacgcggtgc tggctttcaa acgcgaaggc tatcgcaagc gcgacttctc
     2221 atttagtgac acgctggaga ttttgagctc gtcggggatt cgccgggtgc tgcaaaacca
     2281 tctacgctca ggactgggcg agatgaaaaa ctcgctgtgc aaaagcggct acctgcggct
     2341 ggtgcaaaag tattgtcccc ggctttcgtt aagcgatctc cagccctggc ccgccggtgt
     2401 gcgggcgcag gcagtatcgc cggacggcaa gctgattgac gattttctgt ttgtcaccac
     2461 cccacgcacg atccacacct gcaatgcgcc ctccccggca gcgacatcag caattcctat
     2521 tggtgcgcat attgtcagca aggtacaaac gctgttggca agccagagta accccggacg
     2581 cacgctgcga gcggcacgta gtgtggatgc cttacacgcc gcatttaatc aataaccttt
     2641 gaaaacagga cgtaacaatg aaacttaacg acagtaactt attccgccag caggcgttga
     2701 ttaacgggga atggctggac gccaacaatg gcgggatcat cgacgtcacc aatccggcga
     2761 acggcgacaa gctgggtagc gtgccgaaaa tgggcgctga tgaaacccgc gccgctatcg
     2821 acgccgccaa ccgcgccctg cccgcctggc gtgcgctcac cgccaaagaa cgcgccaaca
     2881 ttctgcgcaa ctggttcaat ttgatgatgg agcatcagga cgatttagcg cgcctgatga
     2941 ccctcgaaca gggcaaacca ctggccgaag cgaaaggcga aatcagctac gctgcctctt
     3001 ttattgagtg gtttgccgaa gaaggcaaac gtatttatgg cgataccatt cccggtcatc
     3061 aggccgataa gcgcctgatt gttatcaagc agccgattgg cgttaccgca gctatcacgc
     3121 cgtggaactt cccggcggcg atgattaccc gtaaagccgg tccggcgctg gcggcaggct
     3181 gcaccatggt gctgaagccc gccagtcaga caccgttctc tgcgctggcg ctggcggagc
     3241 tggcgattcg cgcgggcatt ccggctggcg tgtttaacgt agtcaccggt tcggcaggcg
     3301 cggtcggtaa tgaactgacc agcaacccgc tggtacgcaa actgtcgttt accggttcga
     3361 ccgaaattgg ccgccagtta atggaacagt gcgcgaaaga catcaaaaaa gtgtcgctgg
     3421 agctgggcgg caacgcgccg tttatcgtct ttgacgatgc cgatctcgat aaagccgtgg
     3481 aaggcgcgct ggcctcgaaa ttccgcaacg ccgggcaaac ctgcgtctgc gccaaccgtt
     3541 tatacgtgca ggacggcgtg tatgaccgct ttgccgaaaa attgcagcag gcggtgagca
     3601 aactgcacat cggcgacggg ctggataaag gcgtcaccat cgggccgctg atcgatgaaa
     3661 aagcggtagc aaaagtggaa gagcatattg ccgatgcgct ggagaaaggt gcgcgtgtgg
     3721 tttgcggcgg taaagcggat gaacgcggcg gcaacttctt ccagccgacc attctggtgg
     3781 acgtgccggc caacgccaaa gtgtcgaaag aagagacgtt cggccccctc gccccgctgt
     3841 tccgctttaa agatgaagcc gatgtgattg cgcaagccaa tgacaccgag tttggccttg
     3901 ccgcctattt ctacgcccgt gatttaagcc gcgtcttccg cgtgggcgaa gcgctggagt
     3961 acggcatcgt cggcatcaat accggcatta tttccaatga agtggccccg ttcggcggca
     4021 tcaaagcctc gggtctgggt cgtgaaggtt cgaagtatgg catcgaagat tacttagaaa
     4081 tcaaatatat gtgcatcggt ctttaactgg agaatgcgaa tgaacagcaa taaagagtta
     4141 atgcagcgcc gcagtcaggc gattccccgt ggcgttgggc aaattcaccc gattttcgct
     4201 gaccgcgcgg aaaactgccg ggtgtgggac gttgaaggcc gtgagtatct tgatttcgcg
     4261 ggcgggattg cggtgctcaa taccgggcac ctgcatccga aggtggtggc cgcagtggaa
     4321 gcgcagttga aaaaactgtc gcacacctgc ttccaggtgc tggcttacga gccgtatctg
     4381 gagctgtgcg agattatgaa tcagaaggtg ccgggcgatt tcgccaagaa aacgctgctg
     4441 gttacgaccg gttccgaagc ggtggaaaac gcggtaaaaa tcgcccgcgc cgccaccaaa
     4501 cgtagcggca ccatcgcttt tagcggcgcg tatcacgggc gcacgcatta cacgctggcg
     4561 ctgaccggca aggtgaatcc gtactctgcg ggcatggggc tgatgccggg tcatgtttat
     4621 cgcgcgcttt atccttgccc gctgcacggc ataagcgagg atgacgctat cgccagcatc
     4681 caccggatct tcaaaaatga tgccgcgccg gaagatatcg ccgccatcgt gattgagccg
     4741 gttcagggcg aaggcggttt ctacgccgcg tcgccagcct ttatgcagcg tttacgcgct
     4801 ctgtgtgacg agcacgggat catgctgatt gccgatgaag tgcagagcgg cgcggggcgt
     4861 accggcacgc tgtttgcgat ggagcagatg ggcgtggcgc cggatcttac cacctttgcg
     4921 aaatcgatcg caggcggttt cccgctggcg ggcgtcaccg ggcgcgcgga agtgatggac
     4981 gctgttgcac cgggcggtct gggcggcacc tatgccggta acccgattgc ctgcgtggct
     5041 gcgctggaag tgttgaaggt gttcgagcat gaaaatctgc tgcaaaaagc caacgatctg
     5101 gggcagaagc taaaagacgg attgctggcg atagccgaaa aacacccgga gatcggcgac
     5161 gtacgcgggc tgggagcgat gattgccatc gagctgtttg aagatggcga tcacagcaaa
     5221 ccggacgcaa aactcaccgc cgagatcgtg gcacgcgccc gcgataaggg cctgattctt
     5281 ctctcctgcg gcccgtatta caacgtgctg cgcatccttg taccgctcac cattgaagac
     5341 gctcagatcc gtcagggtct ggagatcatc agccagtgtt ttgctgaggc gaagcagtag
     5401 ctatgtccgt ttgtaagagt taaccgttgc taagggaatg ataacgatgc agtaaaaaat
     5461 aataacgatg aggcgaacga caaagttaaa aacgactgcg gacagtcccc tcgcccctct
     5521 ggagagaggg ttagggtgag gggtaaaaca cgcggctaaa ccctcccctg gccctctccc
     5581 taagggagag ggagaaaacc ctcccgcttt cgtttttaag attttgttga ccgtctcaat
     5641 taaacgcgtg acccggcgcg ggaaatgtcg gggcgctctc ccaagtgaca cactttcgag
     5701 aggattcagg atggggcaat catcgcaacc acatgagtta ggcggcgggc tgaaatcacg
     5761 ccacgtcacc atgttgtcta ttgccggtgt tatcggcgca agtctgtttg tcggttccag
     5821 cgtcgccatc gccgaagcgg gcccggcggt attactggcc tatctgttcg ccgggctact
     5881 ggtggttatg attatgcgga tgttggcgga aatggcggtt gccacgcccg ataccggttc
     5941 gttttccacc tatgccgata aagccattgg tcgctgggcg ggctatacca tcggctggtt
     6001 gtactggtgg ttttgggtac tggttatccc gctggaagcc aatatcgccg ctatgatcct
     6061 gcactcgtgg gttccaggca ttcccatctg gttattttcc ctcgtcatta cccttgcctt
     6121 aactggcagt aatttattaa gcgttaaaaa ctacggcgaa tttgagttct ggctggcgct
     6181 gtgcaaagtc atcgctatcc tggcctttat tttccttggt gcagtcgcaa ttagcggttt
     6241 ttacccttat gccgaagtga gcgggatctc aagattgtgg gatagcggcg gctttatgcc
     6301 caacggtttc ggtgcggtat taagcgcgat gttgatcacc atgttctcgt ttatgggcgc
     6361 agaaattgtc accattgccg ccgcggaatc cgacacgccg gaaaaacata ttgtccgcgc
     6421 caccaactcg gttatctggc gtatttctat cttctatttg tgctctattt ttgtcgtcgt
     6481 ggcattaata ccgtggaata tgccggggct gaaagccgtt ggatcttatc gctcggttct
     6541 ggaattgctc aatattcccc atgcgaaatt aatcatggac tgtgtgatat tactttccgt
     6601 aaccagttgc ctgaactcgg ccctgtatac cgcctcaagg atgctctact ccttaagccg
     6661 tcgcggtgat gctcccgcgg taatgggtaa aatcaaccgc agtaaaaccc cgtacgtggc
     6721 ggtgttactc tccaccggcg cggcattctt aacggtggtg gtgaactatt acgcgcctgc
     6781 gaaggtattt aaatttctga tcgacagctc cggcgctatc gccctgctgg tttatttagt
     6841 catcgccgtt tcacagttgc ggatgcgcaa aattctgcga gcggaaggaa gcgaaattcg
     6901 cttgcgaatg tggctttatc cctggctcac ctggctggtc atcggcttta ttacctttgt
     6961 gttggtagtg atgctattcc gtccggcgca acagttagaa gtgatctcca ccggcttatt
     7021 agcgataggg attatctgta ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg
     7081 gcaaaaaaca cccgttcata atacgcgctg ataatgatca ggagtcacac catgaccatt
     7141 acgtctctgg atggctatcg ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg
     7201 gatgaaaaat tacggatgag tttgctgaca tcgcgttatg cacttggcgt tgggccgttg
     7261 cgggaagcac tttctcaact ggtggcggaa cggctggtca cggtggtgaa tcaaaaaggg
     7321 tatcgggtgg cgtctatgtc agagcaggag ctgctcgata ttttcgatgc ccgcgccaat
     7381 atggaagcga tgttagtgag tctggcgatt gcccgcggtg gcgatgagtg ggaggcagac
     7441 gttctcgcaa aagcgcatct gctcagtaag ctggaagcct gtgacgccag cgagaaaatg
     7501 cttgatgagt gggatctgcg tcatcaggcg tttcatacag caattgtggc gggctgtggt
     7561 tctcactatt tgctgcaaat gcgtgaacgg ttgtttgatc tggcggcgcg ctatcgattt
     7621 atctggctgc ggcgaacggt gctttcggta gaaatgctgg aggataaaca cgatcagcac
     7681 cagaccctga ctgcggcggt actggcgcga gataccgcgc gcgccagtga gttaatgcgc
     7741 cagcatttac tgacgccaat tcccattatc cagcaggcga tggctggcaa ttaactactc
     7801 ttccggaata cgcaacactt gccccggata aattttatcc gggcttttta gcatcggttt
     7861 attcgcttcg aagattttat tgtacagatt agcgttaccg tagacctgtt tggaaatggc
     7921 actcagagtg tcgccagact taacggtata aaactggctg gcagtggctg gtgtcgccgt
     7981 tttcacctga tcatcaacac tggcaatacc ggaaatattc cccaccgcaa caaggatttt
     8041 ctccttcgcc tcctgactca ggccgtcacc agtgaccgtc gctttgccgt cggcaatttg
     8101 aatgttcact ttatcagcat caggtatacc ggttttgctc agatgctcct gcaccttctt
     8161 cgcctgatcg tctttatcgt gctgacctgt aaccgcgtcc cagagttttt ctccggcatc
     8221 tttcacaaaa ttgaacagac ccatagctac ctcattcgtt aacggaaaca ctcagaaagt
     8281 gtagcagagg aacgcggcgc gttaacgccg cgggggtgag cacaggttta atcgcgggtt
     8341 tgcacccaga acgcgtgaat caaaccgggg atatacccaa gcagtgtcag cagaatatta
     8401 ataatgaacg cccaaccgaa ccctttaccg agcagcacgc cgagcggcgg tagaatgatg
     8461 gtgatgacga ttctccagaa acccatatgt actccctata agaaaattac
//
LOCUS       DAZOYL010000170         8379 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZOYL010000170 DAZOYL010000000
VERSION     DAZOYL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.171"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHUOY_002775"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHUOY_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR0683509.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(1360..1518)
                     /locus_tag="ACHUOY_002776"
     CDS             complement(1360..1518)
                     /locus_tag="ACHUOY_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0683510.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            2089..2238
                     /locus_tag="ACHUOY_002777"
     CDS             2089..2238
                     /locus_tag="ACHUOY_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683511.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="ACHUOY_002778"
     CDS             complement(2983..3423)
                     /locus_tag="ACHUOY_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR0683512.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="ACHUOY_002779"
     CDS             3641..4030
                     /locus_tag="ACHUOY_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683513.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4262
                     /locus_tag="ACHUOY_002780"
                     /pseudo
     CDS             4074..4262
                     /locus_tag="ACHUOY_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4229..5428)
                     /gene="tet(A)"
                     /locus_tag="ACHUOY_002781"
     CDS             complement(4229..5428)
                     /gene="tet(A)"
                     /locus_tag="ACHUOY_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR0683514.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            5534..6184
                     /gene="tetR(A)"
                     /locus_tag="ACHUOY_002782"
     CDS             5534..6184
                     /gene="tetR(A)"
                     /locus_tag="ACHUOY_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR0683515.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(6216..6458)
                     /locus_tag="ACHUOY_002783"
     CDS             complement(6216..6458)
                     /locus_tag="ACHUOY_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFR0683516.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(6764..7600)
                     /locus_tag="ACHUOY_002784"
     CDS             complement(6764..7600)
                     /locus_tag="ACHUOY_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR0683517.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(7600..>8379)
                     /locus_tag="ACHUOY_002785"
                     /note="aph(3'')-Ib"
     CDS             complement(7600..>8379)
                     /locus_tag="ACHUOY_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR0683518.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1882 a         2316 c         2332 g         1849 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aaagcccgcg acgcagcgcc ggcaggcaga gcaagtagag ggcagcgcct gcaatccatg
     4321 cccacccgtt ccacgttgtt atagaagccg catagatcgc cgtgaagagg aggggtccga
     4381 cgatcgaggt caggctggtg agcgccgcca gtgagccttg cagctgcccc tgacgttcct
     4441 catccacctg cctggacaac attgcttgca gcgccggcat tccgatgcca cccgaagcaa
     4501 gcaggaccat gatcgggaac gccatccatc cccgtgtcgc gaaggcaagc aggatgtagc
     4561 ctgtgccgtc ggcaatcatt ccgagcatga gtgcccgcct ttcgccgagc cgggcggcta
     4621 cagggccggt gatcattgcc tgggcgagtg aatgcagaat gccaaatgcg gcaagcgaaa
     4681 tgccgatcgt ggtcgcgtcc cagtgaaagc gatcctcgcc gaaaatgacc caaagcgcgg
     4741 ccggcacctg tccgacaagt tgcatgatga agaagaccgc catcagggcg gcgacgacgg
     4801 tcatgccccg ggcccaccgg aacgaagcga gcgggttgag agcctcccgg cgtaacggcc
     4861 ggcgttcgcc tttgtgcgac tccggcaaaa ggaaacagcc cgtcaggaaa ttgaggccgt
     4921 tcaaggctgc cgcggcgaag aacggagcgt ggggggagaa accgcccatc agcccaccga
     4981 gcacaggtcc cgcgaccatc ccgaacccga aacaggcgct catgaagccg aagtgccgcg
     5041 cgcgctcatc gccatcagtg atatcggcaa tataagcgcc ggctaccgcc ccagtcgccc
     5101 cggtgatgcc ggccacgatc cgcccgatat agagaaccca aaggaaaggc gccgtcgcca
     5161 tgatggcgta gtcgacagca gcgccggcca gcgagacgag caagaccggc cgccgcccga
     5221 aacgatccga cagcgcgccc agcacaggtg cgcaggcaaa ttgcatcaac gcatacagcg
     5281 ccagcagaat gccatagtgg gcggtgacgt cgttcgagtg aaccagatcg cgcaggaggc
     5341 ccggcagcac cggcataatc aggccgatgc cgacagcgtc gagcgcgaca gtgctcagaa
     5401 ttacgatcag gggtctgttg ggtttcacgt ctggcctccg gaccagcctc cgctggtccg
     5461 attgaacgcg cggattcttt atcactgata agttggtgga catattatgt ttatcagtga
     5521 taaagtgtca agcatgacaa agttgcagcc gaatacagtg atccgtgccg ccctggacct
     5581 gttgaacgag gtcggcgtag acggtctgac gacacgcaaa ctggcggaac ggttgggggt
     5641 tcagcagccg gcgctttact ggcacttcag gaacaagcgg gcgctgctcg acgcactggc
     5701 cgaagccatg ctggcggaga atcatacgca ttcggtgccg agagccgacg acgactggcg
     5761 ctcatttctg atcgggaatg cccgcagctt caggcaggcg ctgctcgcct accgcgatgg
     5821 cgcgcgcatc catgccggca cgcgaccggg cgcaccgcag atggaaacgg ccgacgcgca
     5881 gcttcgcttc ctctgcgagg cgggtttttc ggccggggac gccgtcaatg cgctgatgac
     5941 aatcagctac ttcactgttg gggccgtgct tgaggagcag gccggcgaca gcgatgccgg
     6001 cgagcgcggc ggcaccgttg aacaggctcc gctctcgccg ctgttgcggg ccgcgataga
     6061 cgccttcgac gaagccggtc cggacgcagc gttcgagcag ggactcgcgg tgattgtcga
     6121 tggattggcg aaaaggaggc tcgttgtcag gaacgttgaa ggaccgagaa agggtgacga
     6181 ttgatcagga ccgctgccgg agcgcaaccc actcactaca gcagagccat gtagacaaca
     6241 tcccctcccc ctttccaccg cgtcagacgc ccgtagcagc ccgctacggg ctttttcatg
     6301 ccctgcccta gcgtccaagc ctcacggccg cgctcggcct ctctggcggc cttctggcgc
     6361 tcctgctgcg gcgtccgctc gtgggccgtg gcgcgggtcc gcgcgccggc ctcgtgcgcc
     6421 tggcgctcgc gggcgaggtc cagggcggcc gtcttcacgt tctgccttgc gcagatgaga
     6481 tagatctagc gtggactcaa ggctctcgcg aatggctcgc gttggaaact ttcattgaca
     6541 cttgaggggc accgcaggga aattctcgtc cttgcgagaa ccggctatgt cgtgctgcgc
     6601 atcgagcctg cgcccttggc ttgtctcgcc cctctccgcg tcgctacggg gcttccaggg
     6661 gtcgtttgcg ggaaggggcg gaatcctacg ctaaggcttt ggccagcgat attctccggt
     6721 gagattgatg tgttcccagg ggataggaga agtcgcttga tatctagtat gacgtctgtc
     6781 gcacctgctt gatcgcggcc gcgatagcta gatcgcgttg ctcctcttct ccatccgcgt
     6841 tccaagctgc ggaaaggcac ccataagcgt acgcctggtc gagcaggcga cgcggatcga
     6901 cgtccagcgc acgagagaat gcgtccgcca tctgtgcaat gcgtctagga tcgagacaaa
     6961 ggtcgtctct gtcagccgga tcgtagaaca tattggcggc gccaaagccc acttcaccga
     7021 ccagaccgac gggatctatc accagccagc cgcgactgga gaacatgatg ttttcatgat
     7081 gcagatcgcc atgtagccca cgcagttccg aggcattgct catcatttga tcggctataa
     7141 tcgccgcgtg gacgtagtca gtttgacaac ctgcgttttg atcatcgcgc gcccgctgaa
     7201 acaaagctgc aaagcgatcc cggatcggga gaagggcaga aggcaggggt tcctcagatg
     7261 cggcatacag cttcgccatt agttccgctg caatttcggt cgcctggtag tcgccgtgct
     7321 cggcaacgat gtgagagagc attcgctccc cggcatattc gagcaacatc agattgttct
     7381 cacgaccgag caaccggact gctcccctcc cattgcgcca taccagatag tcggccccgc
     7441 gcagttcatc agcaatgtct tctataggtt tcaatccctt gacgattgca ggagtcccgt
     7501 ctggcaatga aactttccaa acgaggctgg aaaaggtgtc cgcaatgaga acaggttgcg
     7561 aaacgtgcca atgagcagga aaaacaggcg gcatgaacat caaccccaag tcagagggtc
     7621 caatcgcaga tagaaggcaa ggcgttcgcg gtcgggggct tcgatcccca atacattgaa
     7681 taggacagcg aaggcgcgct ctgcttcatc tggcgctgcc cagttctctt cggcgttagc
     7741 aatcatgagt gccaaatcgg catagcgatc tgctgttccg agccgcccaa ggtcgatcag
     7801 acccgtgcat tgaagagttt tagggtccac catgaagttc ggcatgcagg gatcaccatg
     7861 gcaaacaacc atatcggtgc gctcttggtc gagccgcacc ggtagctctc gttcgacacg
     7921 agccaaaaga tcgagctgcg gcgtactctt gtcctcgtcc ggtaagaagt cgggattgac
     7981 ggcattgcgg gacaccacat caacggcgcg tccgaacatt cgcgacagcc tgcgctcaaa
     8041 ctgacattga tcaaccgata ggctgtgaac agcgccaagt tgctgcccca ttgacggcca
     8101 cgctttgagc aaatccgctc cagacagatc agccgccggt actcccggaa ttgccgttat
     8161 caccaagcat gcaccctcct gttcctcctg ccagttgatg acctcggggc aagccacacc
     8221 tcgacctttg agccaaatga ggcggtcacg ctctccagcg agctcaccgc ggcgggaagc
     8281 aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga aaaacaaaat caccagattc
     8341 tccgcctctg acaggcaacc agtcagaatg cgattcacc
//
LOCUS       DAZOYL010000171         8332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZOYL010000171 DAZOYL010000000
VERSION     DAZOYL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.172"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(6..212)
                     /locus_tag="ACHUOY_002786"
     CDS             complement(6..212)
                     /locus_tag="ACHUOY_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR0683519.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            525..614
                     /gene="kdpF"
                     /locus_tag="ACHUOY_002787"
     CDS             525..614
                     /gene="kdpF"
                     /locus_tag="ACHUOY_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR0683520.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            614..2287
                     /gene="kdpA"
                     /locus_tag="ACHUOY_002788"
     CDS             614..2287
                     /gene="kdpA"
                     /locus_tag="ACHUOY_002788"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR0683521.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2310..4358
                     /gene="kdpB"
                     /locus_tag="ACHUOY_002789"
     CDS             2310..4358
                     /gene="kdpB"
                     /locus_tag="ACHUOY_002789"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR0683522.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4367..4939
                     /gene="kdpC"
                     /locus_tag="ACHUOY_002790"
     CDS             4367..4939
                     /gene="kdpC"
                     /locus_tag="ACHUOY_002790"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR0683523.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4932..7616
                     /gene="kdpD"
                     /locus_tag="ACHUOY_002791"
     CDS             4932..7616
                     /gene="kdpD"
                     /locus_tag="ACHUOY_002791"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR0683524.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7613..8290
                     /gene="kdpE"
                     /locus_tag="ACHUOY_002792"
     CDS             7613..8290
                     /gene="kdpE"
                     /locus_tag="ACHUOY_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR0683525.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1728 a         2175 c         2430 g         1999 t
ORIGIN      
        1 agcaatcagt aaaaatcacc ggttgccttt tcggcctgat ccatccacac cggtttatcg
       61 ctggttttcg accagacgcg gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa
      121 agcatgaaag gcagcgccag aacgcccgct gcaaccgcat atgtacggcg taaaaagata
      181 agccatgcag gatattctct gtagagttcc atacttttct cccccttttg tatctacccg
      241 gtgaatagca ccggaaaaat gaatttgttt atctgatgaa aatagtaccg ccttttgtgt
      301 aattttacta ctcatccgac cacttatttt tgcttattga tggtttattt acattcactc
      361 tgtaattaag ttacacaaaa gttaaattaa tactaaacat tagttaaatc atggcttttg
      421 ccatttttat acttttttta caccccgccc gcagattttt gcgaaatctt tgcagccaga
      481 attctactct tccggtatca cttttaggcc actggaggtg cactgtgagt gcaggcgtga
      541 tagccggcgt gttgctggtg tttttattac tgggttattt ggtttatgcc ctgatcaatg
      601 cggaggcgtt ctgatggctg cgcaagggtt cttactgatt gccacgtttt tactggtatt
      661 aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg acattcctct
      721 tccaggtaca gcgggcgttg aacgtatact ctttcgttta cctggcgtct ctgaccatga
      781 gatgaactgg aagcaatatc tttgtgccat tctcggcctg aacatgctgg ggctggcggt
      841 gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc agttgccggg
      901 gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata ccaactggca
      961 atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcaggct taacggtaca
     1021 aaactttctt tctgccgcca gcgggattgc ggtgattttt gccttcatcc gtgcgtttac
     1081 tcgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg
     1141 ggtgctagtc cctgtggcgt tgttgatcgc actgtttttt attcaacaag gtgcgctgca
     1201 aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc tgttacccat
     1261 gggaccagta gcttctcagg aagcgatcaa gatgctcggt actaacggcg gtggcttctt
     1321 taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat
     1381 gctggcgatc ttcttgatcc caacagcgct gtgctttgcc tttggtgaag tgacgggcga
     1441 tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg
     1501 cgtggttatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag
     1561 cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta gcctgtttgc
     1621 ggtcgtgact acggcggcgt cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc
     1681 tctcggcggc atggtgccga tgtggctaat gcaaattggt gaagtggtgt tcggcggtgt
     1741 cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct
     1801 gatgattggt cgtacaccgg aatatctggg taaaaaaatc gacgtacgcg agatgaaact
     1861 gactgcactg gcgattctgg ttactccaac actggtactg atgggcgcgg cgttggcgat
     1921 gatgaccgac gccggacgta gcgccatgct taaccctggc ccgcatggtt ttagcgaagt
     1981 gctgtacgcc gtgtcgtccg ccgctaacaa caacggcagc gcctttgccg gattaagcgc
     2041 caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc gcttcggggt
     2101 gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc aacccgccag
     2161 ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt
     2221 gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg cggaatatct
     2281 ctcctgatga tattgagcga gcactgaata tgagtcgtaa acaactggcg ctattcgaac
     2341 caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat
     2401 ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc tgtattagca
     2461 tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca attagcggct
     2521 ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta
     2581 aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg
     2641 agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt aaaggtgaca
     2701 tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg
     2761 catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt gaatccggcg
     2821 gcgattttgc ctccgtcacc ggcggcacgc gtattctttc tgactggctg gtgattgagt
     2881 gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg gaaggcgcac
     2941 agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct ctgaccatcg
     3001 tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt aatgccgtga
     3061 gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt ggcggcctgt
     3121 tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg attgccacca
     3181 gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat aaaaccggca
     3241 ccatcacact cggtaaccgt caggcgtcgg agtttatccc cgcgcagggc gtggatgaaa
     3301 aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg gaaggtcgca
     3361 gtattgtgat cctcgccaag caacgtttta acctgcgcga gcgcgatgtg cagtcgctcc
     3421 atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaac atcgacaacc
     3481 gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tattgaggct aacggcggtc
     3541 acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcaggga gccacgccgc
     3601 tggtggtggt ggaaggttct cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag
     3661 gcggtattaa agagcgcttc gcccagctgc gcaaaatggg cattaaaacg gtgatgatta
     3721 ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggtgtc gatgattttc
     3781 tcgccgaagc aacaccggag gccaagctgg cattgatccg tcagtatcag gcggaaggtc
     3841 gtttggtagc gatgaccggc gacggcacca acgatgctcc ggcgctggcg caggcagatg
     3901 tcgcggtggc gatgaactcc ggcacccagg cggcgaaaga ggcgggcaat atggtggatc
     3961 tcgactctaa cccgaccaag ttgatcgagg tggtgcacat tggcaaacag atgctgatga
     4021 cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta
     4081 ttccggcggc gttcgcggca acatatccgc agttaaatgc gctgaacatc atgcgcctgc
     4141 attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt
     4201 tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct gccatgttgc
     4261 gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag
     4321 tcattgattt actgctgacc gtttgcggtc tggtgtgagg tttaccatga gtggattacg
     4381 tccggcatta tcgacatttc tctttctgtt attgattact ggcggcgttt acccgctgct
     4441 gaccaccgca ctggggcaat ggtggtttcc ctggcaggcc aacggttcgt tgatccgtga
     4501 aggtgatacg gtgcgtggct cggcattaat cgggcagaat tttaccggca acggctattt
     4561 tcatggtcgc ccgtcagcaa cggctgaaat gccctataat ccacaggctt ctggcgggag
     4621 caatctggcg gtcagtaacc ctgagctgga taaactaata gccgcacgcg ttgctgcatt
     4681 acgggccgct aacccggatg ccagcgcgag cattccggtt gaactggtga cggcatcggc
     4741 aagcgggctg gacaataata tcaccccgca agcggcggcc tggcagatcc cacgcatcgc
     4801 gaaagcgcgt aatctcagcg ttgaacagct cacgcagctg atcgcaaaat acagccaaca
     4861 accgctggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca atctggcgct
     4921 ggataagctt gatgaataac gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa
     4981 ctgccgcgcc gcatcggggg aagctgaaag ttttcttcgg tgcctgtgca ggcgtcggga
     5041 agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg gatattgtgg
     5101 ttggcgtggt agaaacccac gggcgaaaag ataccgccgc aatgctggaa gggctggctg
     5161 ttctgccgcc aaaacgcctg gcgtaccgtg ggcggcacat ctgcgagttt gacctcgatg
     5221 ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac agtaatgcgc
     5281 caggttctcg tcaccctaaa cgctggcagg acatcgaaga gctgctggaa gcaggcattg
     5341 atgttttcac taccgtcaac gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg
     5401 tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg
     5461 tggttctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct
     5521 atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc aatctgatcg
     5581 ccctgcgcga actggcactg cgccgtactg ccgatcgcgt tgatgagcaa atgcgcgcct
     5641 ggcgggggca tcctagcgaa gagaaagtct ggcacacgcg cgacgcgatc cttttatgca
     5701 tcggccataa caccggtagt gagaagctgg tccgcgcggc ggcgcggctg gcgtcacggc
     5761 tgggtagcgt ctggcacgcg gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga
     5821 aaaaacgtcg tgcaattctc agcgccttac gtctggcgca ggaactgggc gcggagacgg
     5881 caacactttc tgatccagcg gaagagaaag cggtagtgcg ttatgcccgt gaacataatc
     5941 tcggcaagat tattctcggt cgcccggcct cgcgccgctg gtggcgtcgg gaaacgtttg
     6001 ctgaccgact ggcgcgcatc gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac
     6061 cacccgcccg cacgattaac aacgcgccgg atagccgctc ttttaaagac aagtggcggg
     6121 tacagattca gggatgcgtg gttgccgccg cgttatgcgc cgttatcacc ttaattgcca
     6181 tgcagtggct gatggcgttt gatgccgcca acctggtgat gctgtatctg cttggcgtgg
     6241 tggtggtggc gctattttat ggacgctggc cttcggtggt tgccaccgtc attaatgtgg
     6301 tgagtttcga tctctttttt atcgccccac gcggcacgct cgccgtctct gatgtgcaat
     6361 atctgctgac cttcgcggtg atgttaaccg tcgggctggt gatcgggaac cttactgctg
     6421 gcgtgcgtta tcaggcgcgg gtagcccgtt accgcgagca acgcacacgg cacttatatg
     6481 aaatgtcgaa agctctggcg gtgggccgca gtccgcagga catcgccgcc accagcgaac
     6541 aatttattgc ctccacgttt catgcccgca gtcaggtgtt gttgcccgat gacaacggta
     6601 aattgcagcc gttaacacat ccgcaaggaa tgacgccatg ggacgatgcc atcgcgcagt
     6661 ggagttttga taaaggcctg cctgcgggcg cgggcaccga cacgttaccc ggtgtaccgt
     6721 accagatttt gccgctaaaa agcggcgaga aaacctacgg gctggtggtg gtggagccgg
     6781 ggaatctgcg ccagttgatg atcccggaac agcagcgcct gctggagacg tttacgctgt
     6841 tagtcgccaa tgcccttgag cggctgacgc taaccgccag cgaagaacag gcgcggatgg
     6901 caagcgaacg tgaacagatc cgcaacgccc tgctggcagc gctttcgcat gatttacgca
     6961 cgccgcttac ggtgctgttt ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag
     7021 gatcacccca cgcccgccag gccagcgaga ttcgtcagca tgtgctgaac actacccgac
     7081 tggtgaataa tctactggat atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag
     7141 agtggttaac gctggaagaa gtagtcggca gcgcgctgca aatgctggaa ccgggtttat
     7201 cgtcgcccat caatctttct ctgccagaac cgctgacctt aatccacgtt gacgggccac
     7261 tctttgaacg ggtgctgatt aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg
     7321 ccgaaattgg tatcgatgcc cactttgagg gcgaaaatct acaactggat gtctgggata
     7381 acggccccgg tcttccgcca ggccaggagc agacgatatt tgataagttt gctcgcggga
     7441 ataaagagtc ggcggtgccg ggtgtcgggc ttgggttggc aatttgtcgg gcgattgtgg
     7501 atgtacacgg cggcaccatt accgcgttca accgaccgga aggtggtgcc tgttttcgtg
     7561 ttacacttcc ccagcaaacc gctcctgaac ttgaagactt tcatgaggat atgtgacaaa
     7621 cgttctgatt gttgaagatg aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg
     7681 cgacgggatg cgcgtctttg aggccgaaac tctgcaacgc ggcttgctgg aagcggcaac
     7741 ccgtaagcca gatttgatta ttctcgatct cggcctgccc gatggtgatg ggattgagtt
     7801 tatccgcgac ctgcgccagt ggagcccggt gccggtgatt gtgctttccg cacgcagcga
     7861 agagagcgac aagatcgccg cgctggatgc cggagcggat gattatctga gtaagccgtt
     7921 tggcattggc gaattgcagg cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc
     7981 cgcgcccgat ccgctggtga aattttccga tgttaccgtc gatttagccg cccgcgtgat
     8041 tcaccggggt gaggaagagg tgcatctcac accaattgag ttccgcctgc tggcggtgct
     8101 gctcaacaat gccggaaaag tactcaccca gcgccagctc cttaaccagg tgtgggggcc
     8161 aaacgcggtc gaacacagtc actatttgcg tatttatatg ggacatctgc gacaaaaact
     8221 ggaacaggat ccggcccggc ctcgccactt cattactgaa accggtattg gatatcggtt
     8281 tatgctttga attgttattc aataaattcg cttaaatagt gattaattta tt
//
LOCUS       DAZOYL010000172         8331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZOYL010000172 DAZOYL010000000
VERSION     DAZOYL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.173"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..188
                     /locus_tag="ACHUOY_002793"
     CDS             <1..188
                     /locus_tag="ACHUOY_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683526.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
     gene            411..908
                     /gene="ubiC"
                     /locus_tag="ACHUOY_002794"
     CDS             411..908
                     /gene="ubiC"
                     /locus_tag="ACHUOY_002794"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR0683527.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUOY_002795"
     CDS             921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUOY_002795"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR0683528.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUOY_002796"
     CDS             complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUOY_002796"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR0683529.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUOY_002797"
     CDS             4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUOY_002797"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR0683530.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUOY_002798"
     CDS             5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUOY_002798"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR0683531.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUOY_002799"
     CDS             5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUOY_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR0683532.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7142..7351
                     /locus_tag="ACHUOY_002800"
     CDS             7142..7351
                     /locus_tag="ACHUOY_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR0683533.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUOY_002801"
     CDS             complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUOY_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR0683534.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8226..>8331
                     /gene="yjbL"
                     /locus_tag="ACHUOY_002802"
     CDS             8226..>8331
                     /gene="yjbL"
                     /locus_tag="ACHUOY_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR0683535.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRG"
BASE COUNT         1919 a         2013 c         2260 g         2139 t
ORIGIN      
        1 atatgttgaa tcctacaaaa gataccaatt ggaattccac gtgtatctat aaatccagac
       61 ataagatgtt gcctattaat cttactcagg aaacactttt tagctccaaa tctcatggta
      121 aatatgcgct tttcccaatt tttactgcga gttggcgagc tactcgtata aagaatatag
      181 gtatttaagt aaaggaaaac atcaccgttc ctggcatcat gggcggtgat gcccctacgg
      241 ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc aacagcgttt taccacttca
      301 tgcgattgtt gcgtttttgt tgcgcattag atcacttaat ttgctttaca tctcccgtaa
      361 acacttttct gcgatacaat gcctttacgt tatgtaacgg agagttcggc atgtcacacc
      421 ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga gatccctgcc ctggattcgc
      481 aactgctcga ctggctgttg ctggaagatt ccatgacaaa acgttttgaa cagcagggaa
      541 aaacggtgag cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa atccccgaag
      601 aactgccgct gctgccgaaa gagtctcgtt actggttacg tgaaattttg ttatgtgccg
      661 atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg
      721 agctggcgtt acaaaaattg ggtaaaacgc cgctaggacg ctatctgttc acatcatcga
      781 cattaacccg ggactttatt gagataggcc gtgatgccgg actgtggggg cgacgttccc
      841 gcctgcgatt aagcggtaaa ccgctgttgc taacagaact gtttttaccg gcgtcaccgt
      901 tgtactaaga ggaaaaaaat atggagtgga gtctgacgca gaataagctg ctggcgtttc
      961 atcgcttaat gcgtacggat aagccaattg gcgcgttact gctgctctgg ccaacattat
     1021 gggcgttgtg ggtggcgaca ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg
     1081 cgggtgtctg gctgatgcgc gctgccggat gtgtggtgaa tgattatgct gaccgcaagt
     1141 ttgatggtca tgttaagcgc acggcgaacc gaccgcttcc cagcggcgcg gtaacggaga
     1201 aagaggcgcg cgcgctgttt gtagtgctgg tattgatttc atttttactg gtgctgacgc
     1261 tgaatacgat gaccattctg ttgtcgattg ccgctctggc gttggcgtgg gtgtacccgt
     1321 ttatgaagcg gtatacccat ctcccgcaag tggtgctggg cgcggcgttt ggctggtcga
     1381 ttccaatggc ttttgccgct gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc
     1441 tcgccaatat tctctgggcg gtggcttacg acacgcagta tgcaatggtt gaccgtgacg
     1501 atgatgtgaa gattggtatt aaatccaccg caatcctgtt cggccaatac gataaattga
     1561 ttattggtat tttgcagatt ggcgtactgg cactgatggc gatcatcggt gagttaaatg
     1621 gcttaggctg gggatattac tggtcaattc tggtggctgg cgcgctgttt gtttatcaac
     1681 aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa agcatttatg aataataact
     1741 atgttggtct ggtactgttt ttaggactgg caatgagtta ctggcatttc tgatgatgta
     1801 aaaaagccgg atgatcatcc ggctttcttc tgggttgcct gatgcgctgc gcttctcagg
     1861 cctacacaac acatcgcaat ttattgaatt tgcagattat ggaaggccgg ataaggcgtt
     1921 ttcgccgcat ccggcaattc tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt
     1981 caaacgcacg tctgatgtaa tcaactccgc cagcaactga taaaccttca tcgtttctgc
     2041 cggttcggcg tcgccgctat cgctgatata cccttcatca cgcagcgtca gcaccagaga
     2101 actgaacacc gccttgtcga agaactccgg cgcgttgatg ccgtgcagca cggagagacg
     2161 ttgcgcaacg gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg ggttggcact
     2221 caacaaccag aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg cggccagcag
     2281 ctgtaggggg cgagaatgcg ccgggttgat atgcaactca tcatcttgca gggtaatcag
     2341 cccctgacgt tgcatctcat ttgccagcgc atcaataacg tccggcaact cgtcgcgatc
     2401 ccagcgcagg aacagctccg ctttcagcat tgggtaaagc acattgacgt gctccatcaa
     2461 tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc gccatcagcg aaggcagcac
     2521 caacatatgc gcaatgttgt tgcgatagta ggtcatcagc accgcttgct cgcgcggcag
     2581 aatgatgatg tcgccgatag tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg
     2641 atcgataagc tcgctggcgc tggctgaagg aacggtagag tccgtggagt aaggtacgtt
     2701 gcgcatcaga tccaggtagc agttgagttg ctcggttaac tgctcgcggg tgagtgagcg
     2761 ctgacgtgat gccagaagcg cagtacaaca caggttcatg gcgtttgccg cgcctgcgtt
     2821 gttaatgcgt accatcagat cggcagcaat attattgacc gtcggcgtta accaggccgg
     2881 acgcaccgct tcgatgggat cgatagattc acgccagtct ggtacgtgct ggttaaggta
     2941 ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc tgaccgagat tacgcagctt
     3001 gcttaaaccg cgcagcatct gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc
     3061 tttggcgtaa gtacccactt ccatgacgtg ctcataaccg atatagatcg gaatcagcgt
     3121 aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg taccggtttt
     3181 cggatccagc aaacgccccg tgcgggaacg accgccttcc acgaagtact cgacggaata
     3241 accacggctg aacagttcgc cgagatactc acggaaaacg gtggaataaa gtttattgcc
     3301 tttaaaagta cggcgaataa agaacgcccc cagacggcgg aaaatcggcc cggccggcca
     3361 gaagttcagg ttgatcccgg cggcgatatg cggcggcacc agcccctggt ggtacagcac
     3421 gtaagaaagt agcaggtagt ccatgtgact gcggtggcaa ggcacataca ccagctcatg
     3481 accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg acgttgatgc cctgataaag
     3541 tcggttccag gtgaagccca gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt
     3601 cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc gctttttcat gggagatttt
     3661 tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt
     3721 aaacagatcc tgacgagcag gcagacgtgg gcctacggca gccagacgtt gacgggcaaa
     3781 gtgcatacgc gccacgcgcg ccagtttctg tgcgatagtt ttatccgtgc cgtgttcatc
     3841 cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca
     3901 cagtacggcg aaaaatttct gtacgccgtt aagcatacgc agcggcgggt ttacttcgcc
     3961 tttttcacgc cccggcgcgc gaccaaacat caccgatact ggcaccatct gcacatccag
     4021 atttgggttg ctacggtgca aatcgagata gtcgtggaac agcttaatag actcttcttt
     4081 cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg aacacatagc gcggcagtag
     4141 cgtgccgtcg atttctagcg gctctaacgg gtcaggcaag tcatgagcca gacactgggc
     4201 gcgtaacgtc agcaaatctg ctttcgagtt gtacggtaaa acgtacataa ttggacgaga
     4261 ggtatccagc cccagttccg gggcaggatc tgccggaata gacttgcttt ttaccaggat
     4321 gcttaatggt aaattcagta atttgtagta aattcgtggc cagccggaca taaacgatgt
     4381 aaagcctctg gttaataatg caaatgcgcg gcaaggatag cagaaagtca tgggaaattc
     4441 tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg
     4501 tacactgata ttgacgctca taatgtaaaa aggttctttc aatggccaat aataccactg
     4561 gattcacccg aattatcaaa gctgctggct attcctggaa aggtttacgc gctgcatgga
     4621 tcaacgaagc ggcattccgt caggaaggcg tagcggtatt gttggcggtg gtcatcgcct
     4681 gctggctgga tgtggacgca attacccgcg tgctgcttat cagctccgtg atgctggtga
     4741 tgattgtgga aattctcaat agcgccatcg aagcagtggt tgaccgaatt ggctctgaat
     4801 accatgagct ttccggacgc gctaaagata tgggatccgc cgcggtgctg attgcgatta
     4861 tcgtcgccgt gataacctgg tgcatcctgt tatggtcgca ttttggataa cccttccaga
     4921 tttcgataaa tctctggttt attgtgcagt ttatggttcc aaaatcgcct tttgctgtat
     4981 atactcacag cataactgta tatacaccca gggggcggaa tgaaagcgtt aacggccagg
     5041 caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg
     5101 cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg
     5161 aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt
     5221 ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg
     5281 cttctggcgc agcagcatat tgaaggtcat tatcaggtcg atccttcctt gttcaagccg
     5341 aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg cattatggat
     5401 ggtgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca
     5461 cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac agggtaataa agtcgaactg
     5521 ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc
     5581 attgaagggc tggcggttgg ggttattcgc aacggcgact ggctgtaaca tatctctgag
     5641 accgcgatgc cgccaggcgt cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc
     5701 atggcattcc tcacttcatc tgataaagca ctctggcatc tcgccttacc catgattttc
     5761 tccaatatca ccgttccgtt gctgggactg gtcgatacgg cggtaattgg tcatcttgat
     5821 agcccggttt atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg
     5881 ctgttgctgt ttttacgcat gagcaccacc gggctgactg cgcaggctta tggtgccaaa
     5941 aatcctcagg cattagcccg tgcgctggtg caaccgttgc tgttggcgtt gggggctggg
     6001 gcgttaattg cgctgctgcg tacaccgatt atcgatctgg ctctgcatat tgttggcggt
     6061 agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa tccgctggtt aagcgcaccg
     6121 gcgtcgctgg cgaatctggt attactcggt tggttactcg gcgtgcaata tgcccgtgcg
     6181 ccagtaattt tgttagtggt cggcaatatc ctcaacattg tgctggatgt ctggctagtg
     6241 atggggctgc atatgaacgt gcagggcgcg gctctggcga cggttattgc ggaatatgca
     6301 acattgctga ttggtctgct aatggtgcgt aaaatcctca aactacgcgg aatttccggc
     6361 gaaatgtcga aaactgcctg gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat
     6421 atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg
     6481 cgactgggga gtgacattat cgctgtaaac gcggttctga tgacgctact cacctttacc
     6541 gcctatgcgc tggatggttt tgcctacgcg gttgaagcac attccggtca ggcatacggt
     6601 gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag cgtgccgcca gtcggggatc
     6661 gtagcgttac tattttcggt ggtttatttg ctggcagggg aacacatcat tgcgttgctg
     6721 acgtcgttaa cccagattca gcagctggct gaccgctatc ttatctggca ggtgattttg
     6781 ccgttggttg gcgtctggtg ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc
     6841 gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg
     6901 ctgccgtggc tgggtaatca tggtttgtgg ctggcactaa ccgtctttct ggcgttgcgc
     6961 gggctttctc tggcggctat ctggcggcgt cactggcgta acggtacctg gtttgccgca
     7021 acgtgacggt taaaaattct gaataaatta tcctaagcca aattgctgac tacacttaat
     7081 ctcacgttca gaagaaaagt gaacgtactc tcattcacaa cctaacgatg aggtcttgat
     7141 tatgaataaa gatgaagccg gcggtaactg gaaacagttt aaaggtaaag tgaaagagca
     7201 atggggcaaa ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg atcaactggt
     7261 cggtaaaatc caggaacgtt atggttatca gaaagatcag gcagaaaaag aggtcgtgga
     7321 ttgggaaacc cgcaatgaat atcgctggta attaatccct cctgcccgac gtgtacaagg
     7381 atgtacgccc tcttaacgcg gtttcttttt cacctgcaca gagtgatcat gctggcactg
     7441 ttcaggatga cgacacgctt ccacttccac gcatgccgca cagagcccat gtgcttcaat
     7501 cacattatgc cgcagggcaa accccatttt tgccgccagc gtatgcataa tgtcttccac
     7561 gccttctgca cactcttctt tcaccgcgcc gcagcgatcg catataaaca tggctgacgt
     7621 atgcgtgggc tggtcgaaca gatgacagag cacataactg ttggtggatt ccaccttatg
     7681 cacaaaacct tgctcaagca gaaaatccag cgcgcgataa accgttggcg gcttggcttg
     7741 cggttcagct tcgcgcagta aatcaagcag atcataagcg ctgatagcgc catcctgcag
     7801 actcatcagg cgcaacactt ccaggcgctg tggggtcagg cgcacattac gctgcgcgca
     7861 gattttttca gcctgcgcta ataactcctg cgttgtggtc ttttccatcg ggcacctcaa
     7921 agtgggggtt aagaatgtcc ttactttacc atgttccagg aaaaacaccg agaaccctta
     7981 ttgttgccgt aatgttgatt ttctgttttg taggtaaggt gttatgttgc cttgtcgtac
     8041 cattatcaac acgataataa ttaataattc atttttaaat aaattgcttc aagttaactc
     8101 gctatttatt taactgggtt taaacgcata cctgtagttc agaaattatg agcgattttt
     8161 gatagtgacc agatggcaaa agaattataa ggaggacacg gtacgacaaa aaaggtagat
     8221 gaatcatgtt gaagataata cccggtgcaa ctggatactt taataaaaca ctcaattcaa
     8281 atcaattcga taatgaagat gcaatcaaag ataaattaga taatagaggt t
//
LOCUS       DAZOYL010000173         8292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZOYL010000173 DAZOYL010000000
VERSION     DAZOYL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.174"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(194..1084)
                     /gene="malG"
                     /locus_tag="ACHUOY_002803"
     CDS             complement(194..1084)
                     /gene="malG"
                     /locus_tag="ACHUOY_002803"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR0683536.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1099..2643)
                     /gene="malF"
                     /locus_tag="ACHUOY_002804"
     CDS             complement(1099..2643)
                     /gene="malF"
                     /locus_tag="ACHUOY_002804"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR0683537.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2797..3987)
                     /gene="malE"
                     /locus_tag="ACHUOY_002805"
     CDS             complement(2797..3987)
                     /gene="malE"
                     /locus_tag="ACHUOY_002805"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR0683538.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4352..5467
                     /gene="malK"
                     /locus_tag="ACHUOY_002806"
     CDS             4352..5467
                     /gene="malK"
                     /locus_tag="ACHUOY_002806"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR0683539.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5539..6879
                     /gene="lamB"
                     /locus_tag="ACHUOY_002807"
     CDS             5539..6879
                     /gene="lamB"
                     /locus_tag="ACHUOY_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR0683540.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7031..7951
                     /gene="malM"
                     /locus_tag="ACHUOY_002808"
     CDS             7031..7951
                     /gene="malM"
                     /locus_tag="ACHUOY_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR0683541.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVSYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            8180..>8292
                     /locus_tag="ACHUOY_002809"
     CDS             8180..>8292
                     /locus_tag="ACHUOY_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683542.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVT"
BASE COUNT         2053 a         2147 c         2146 g         1946 t
ORIGIN      
        1 aaagagaatt atcgtaagtg ggaaaacaaa taacgtaaaa ataataagct ctatgatgga
       61 aatattaacc ggcgaacgat tcagattgca gacgaaagaa aaaaaggcgc tccgtggagc
      121 gccgaataac agtcacaagt tgggataacg taagttgagg gtgcagcggc ataacattgg
      181 cagaacaaca tctttaacct ttcacaccac ctgccgtcag gccgttgacc agccagcgtt
      241 gagccagcaa gaagacgatg gtgatcggta atgcagacat cacggcagcg gcggcaaagt
      301 caccccacag gtagttttgc gggttgaggt attgctgcat ccccacggcc agggtgtagc
      361 tgtttacgtc acgcagtaac agcgacgcga ccggaacttc agtaatggca gcgataaacg
      421 acaggatgaa taccaccgcc agaatcggta ctgacagcgg caacaggaca aggcggaagg
      481 cctgccacgg tgtcgcacca tccagcgcag ccgcttcttc cagcgaacta tcgatggttt
      541 cgaaatagcc tttgatggtc cagacatgca gcgcaatccc acccagatac gcgaaaatta
      601 cgccgccgtg agtattcagg ccaatgaatg gaatgtactc acccagacga tcaaacaacg
      661 catacaacgc gaccagtgaa agtactgccg ggaacatctg gaaaatcagc attcctttca
      721 gcagcgtcgc tttgcctgga aagcgcatac gggcgaaagc gtaggcgcag gtggtagaga
      781 gcgcgacaat gccaatcgcg gaaatcccgg cgacctttac cgagttccat agccacagca
      841 gtaccgggaa tggcggtggc gtaatgcgac catcagcctg ttccacgcta aaacctaaag
      901 ccagtttcca gtgatcccag gagatttgct ccgggatcag gctgccgatc gcaaagttcc
      961 cctggcgcag tgagatagcg acgaccatca gcagcgggaa cataatcgcc gcgataaaaa
     1021 gtagcagtag caggtgagta ataaacaaac gtgctttttg cgatttcggt tggaccattg
     1081 ccatttttgt tatctccctt aatcaaactt cattcgcgtg gctttcaggt tcactatcgc
     1141 cagcgcaccc accagcagga agagcagcgt ggcaattgct gcagccagac cgaagtcctg
     1201 acccccgccg ccttcaaaag cgatacggta ggtgtagtta acaagcaggt cggtataacc
     1261 ggctggcgtg gtcgtgccaa gacgatccgg gccgccgttg gttaacagtt gaatcagcac
     1321 gaagttgtta aagttaaagg cgaagctggc gatcatcagc ggcgtcagcg gtttaatcag
     1381 cagcggcagc gtaatcttaa agaagttctg gaacggatct gcgccatcca ttgctgaggc
     1441 ttcatacaaa tcgtccggaa tcgctttcag caagcccatg cagaggatca tcatgtacgg
     1501 ataacccagc caggtattga cgataattag catcgtgcgg gcggtggtcg gatcgctgaa
     1561 ccaggcgggc ttcacgccaa acagcgcgct caacatcatg ttgatttcac cgaagctctg
     1621 gttaaacaac cctttgaaaa tcaagattga aatgaatgat ggcaccgcgt agggcagaat
     1681 cagcagaacg cgatagaccg ctttgccgcg caatgcttcc cactgcacca gacatgccag
     1741 aaccatgccg accgccaccg ttaaaaagac agtgatcagc gagaacacca cggtccagac
     1801 gaaaatggcg aggaacggtt tctgaatgcc ttcgtcggtg aagacgcggg taaagttttt
     1861 ccagccagta gtcacggtgt aaccggggct tagcttttca tcaccccagt tgccgtcggc
     1921 ggtaatggac tggtaaaagc caatttggtt attcggacga tatttcacgc cgctctgatt
     1981 attcgtcaac gtgccgtcac cgtcgagtgt gtagagcggc tgcgtgccag aaaactggcg
     2041 cagggagctc atcatcactt tgttgccatc cggcagaatg gcggtaatgt cactcagcgc
     2101 ctgacgattc tgggtaatca cgcgtagatt cgcgcgttcg ccttcgggct gggcggtcgt
     2161 ttctttcagt tgcagttttt gttcgccgcc aaatttaaac gcgtcggaga ggtaattttt
     2221 gccgatttcg ccgtcactga gcgccagttg ccactcatcg cccgccgggt aaagaccaaa
     2281 gttataggtt ttgcctgctt gccaggagcg atctaacaac acttcctgcg cacattcaaa
     2341 agtcagctgg ttagtgctgc tgtagttggt gaaggcaatg gcgatggtgc agaccagagg
     2401 gaagaggacg aataatccca ttccagccat tcccgggtaa acatagcgcc aggcgtaggc
     2461 tttacgattg gcgaaaatat acagccccgc tgaactcaat atcagcgtgg taatggcgaa
     2521 caggtattcc ccttgtgcgt acattaaaac aacaaggtaa cccaccagca ggccgagcag
     2581 acctagcact gaccatttca gcgcgtcgct ttgccaccaa tgtttctttt taatgacatc
     2641 catggggttc ttcctcattc caggacggat aaggctttca cgccttatcc gacaacaact
     2701 gcctgatgcg acactgatgc gtcttatcag gcctacatgc gtttcagttt tgtaggccgg
     2761 acaaggcgtt cacgccgcat ccggcatttc acagcattac ttggtgatac gagtctgcgc
     2821 gtctttcagg gcttcatcga cagtctgacg accgctggcg gcgttgatca ccgcagtacg
     2881 cacggcatac cagaaagcgg acatctgcgg gatgttcggc atgatttcac ctttctgggc
     2941 gttttccatg gtggcggcaa tacgtggatc tttcgccaac tcttcctcgt aagacttcag
     3001 cgccactgca cccagcggtt tgtctttatt gaccgcttcc agaccttcgt cagtcagcag
     3061 atagttttcg aggaactctt tcgccagctc tttgttcgga ctggcggcgt taataccggc
     3121 gctcagcacg ccaacgaacg gtttagatgg ttgacccttg aaggtcggca gtaccgttac
     3181 accataattc actttgctgg tgtcgatgtt ggaccatgcc cacgggccgt tgatggtcat
     3241 cgctgtttcg cctttattaa aggcagcttc tgcgatggag taatcggtgt ctgcattcat
     3301 gtgtttgttt ttaataaggt caaccaggaa ggtcagaccc gctttcgcgc cagcgttatc
     3361 cacggccacg tctttaatat cgtacttgcc gttttcatac ttgaacgcat aacccccgtc
     3421 agcagcaatc agcggccagg tgaagtacgg ttcttgcagg ttgaacatca gcgcgctctt
     3481 acctttcgct ttcagttctt tatccagcgc cgggatctct tcccaggttt ttggtgggtt
     3541 cggcagcaga tctttgttat aaatcagcga taacgcttca acagcgatcg ggtaagcaat
     3601 cagcttgccg ttgtaacgta cggcatccca ggtaaacgga tacagcttgt cctggaacgc
     3661 tttgtccggg gtgatttcag ccaacaggcc agattgagcg tagccaccaa aacggtcgtg
     3721 cgcccagaag ataatgtcag ggccatcgcc agttgccgca acctgaggga atttctcttc
     3781 cagtttatcc ggatgctcaa cggtgacttt aattccggta tctttctcga atttcttacc
     3841 gacttcagcg agaccgttat agcctttatc gccgttaatc cagattacca gtttaccttc
     3901 ttcgattttg gcgagagccg aggcggaaaa catcatcgtc gttaatgcgg ataatgcgag
     3961 gatgcgtgca cctgttttta ttttcataat ctatggtcct tgttggtgaa gtgctcgtga
     4021 aaacacctaa acggactcta gtttctttat acggcaacct ctttccatcc tccttgcccc
     4081 tacgccccac cgttgctttg tgtgatctct gttacagaat tggcggtaat gtggagatgc
     4141 gcacataaaa tcgccacgat ttttgcaagc aacatcacga aattccttac atgacctcgg
     4201 tttagttcac agaagccgtg ttctcatcct cccgcctcct cccccataaa aaagccaggg
     4261 ggtggaggat ttaagccatc tcctgatgac gcatagtcag cccatcatga atgttgctgt
     4321 cgatgacagg ttgttacaaa gggagaaggg catggcgagc gtacagctgc aaaatgtaac
     4381 gaaagcctgg ggcgaggtcg tggtatcgaa agatatcaat ctcgatatcc atgaaggtga
     4441 attcgtggtg tttgtcggac cgtctggctg cggtaaatcg actttactgc gcatgattgc
     4501 cgggcttgag acgatcacca gcggcgacct gttcatcggt gagaaacgga tgaatgacac
     4561 tccgccagca gaacgcggcg ttggtatggt gtttcagtct tacgcgctct atccccacct
     4621 gtcagtagca gaaaacatgt catttggcct gaaactggct ggcgcaaaaa aagaggtgat
     4681 taaccaacgc gtcaaccagg tggcggaagt gctgcaactg gcgcatttgc tggatcgcaa
     4741 accgaaagcg ctctccggtg gtcagcgtca gcgtgtggcg attggccgta cgctggtggc
     4801 cgagccaagc gtatttttgc tcgatgaacc gctctccaac ctcgatgctg cactgcgtgt
     4861 gcaaatgcgt atcgaaatct cccgtctgca taaacgcctg ggccgcacaa tgatttacgt
     4921 cacccacgat caggtcgaag cgatgacgct ggccgacaaa atcgtggtgc tggacgccgg
     4981 tcgcgtggcg caggttggga aaccgctgga gctgtaccac tatccggcag accgttttgt
     5041 cgccggattt atcggttcgc caaagatgaa cttcctgccg gtaaaagtga ccgccaccgc
     5101 aatcgatcaa gtgcaggtgg agctgccgat gccaaatcgt cagcaagtct ggctgccagt
     5161 tgaaagccgt gatgtccagg ttggagccaa tatgtcgctg ggtattcgcc cggaacatct
     5221 actgccgagt gatatcgctg acgtcatcct tgagggtgaa gttcaggtcg tcgagcaact
     5281 cggcaacgaa acccaaatcc atatccagat cccttccatt cgtcaaaacc tggtgtaccg
     5341 ccagaacgac gtggtgttgg tagaagaagg tgccacattc gctatcggcc tgccgccaga
     5401 gcgttgccat ctgttccgtg aggatggcac tgcatgtcgt cgactgcata aggagccggg
     5461 cgtttaagca ccccacaaaa cacacaaagc ctgtcacagg tgatgtgaaa aaagaaaagc
     5521 aatgactcag gagatagaat gatgattact ctgcgcaaac ttcctctggc ggttgccgtc
     5581 gcagcgggcg taatgtctgc tcaggcaatg gctgttgatt tccacggcta tgcacgttcc
     5641 ggtattggct ggacaggtag cggcggtgaa caacagtgtt tccagactac cggtgctcaa
     5701 agtaaatacc gtcttggcaa cgaatgtgaa acttatgctg aattaaaatt gggtcaggaa
     5761 gtgtggaaag agggcgataa gagcttctat ttcgacacta acgtggccta ttccgtcgcg
     5821 caacagaatg actgggaagc taccgatccg gccttccgtg aagcaaacgt gcagggtaaa
     5881 aacctgatcg aatggctgcc aggttccacc atctgggcag gtaagcgctt ctaccaacgt
     5941 catgacgttc atatgatcga cttctactac tgggatattt ctggtcctgg tgccggtctg
     6001 gaaaacatcg atgttggctt cggtaaactc tctctggcag caacccgctc ctctgaagct
     6061 ggtggttctt cctcttttgc cagcaacaat atttatgact ataccaacga aaccgcgaac
     6121 gacgttttcg atgtgcgttt agcgcagatg gaaatcaacc cgggcggcac attagaactg
     6181 ggtgtcgact acggtcgtgc caacctgcgt gataactatc gtctggttga tggcgcatcg
     6241 aaagacggct ggttattcac tgctgaacat actcagagtg tcctgaaggg ctttaacaag
     6301 tttgttgttc agtacgctac tgactcgatg acctcgcagg gtaaaggtct gtcgcagggt
     6361 tctggcgtcg cgtttgataa cgaaaaattt gcctacaata tcaacaacaa cggtcacatg
     6421 ctgcgtatcc tcgaccacgg tgcgatctcc atgggcgaca actgggacat gatgtacgtg
     6481 ggtatgtacc aggatatcaa ctgggataac gacaacggca ccaagtggtg gaccgtcggt
     6541 attcgcccga tgtacaagtg gacgccaatc atgagcaccg tgatggaaat cggctacgac
     6601 aacgtcgaat cccagcgcac cggcgacaag aacaatcagt acaaaattac cctcgcacaa
     6661 caatggcagg ctggcgacag catctggtca cgcccggcta ttcgtgtctt cgcaacctac
     6721 gccaagtggg atgagaaatg gggttacgac tacaacggcg atagcaaggt taacccgaac
     6781 tacggcaaag ccgttcctgc tgatttcaac ggcggcagct tcggtcgtgg cgacagcgac
     6841 gagtggacct tcggtgccca gatggaaatc tggtggtaat agcaaaacct gggccggata
     6901 aggcgtttac gccgcatccg gcataaaaac aggttgtcat tatctgaaag gggcgaaagc
     6961 ccctctgatt atcgggttta gcgcgctatt gcctggctac cgctgagctc cagattttga
     7021 ggtgaaaaca atgaaaatga ataaaagtct catcgccctc tgtttatcag cagggttact
     7081 ggcaagcgcg cctggaatta gccttgccga tgttagctac gtaccgcaaa acaccagcga
     7141 cgcgccagcc attccatctg ccgcgctgca acaactcacc tggacaccgg tcgatcaatc
     7201 taaaacccag accacccaac tggcgaccgg cggccaacaa ctgaacgttc ccggcatcag
     7261 tggtccggtt gctgcgtaca gcgtcccggc aaacattggc gaactgaccc tgacgctgac
     7321 cagcgaagtg aacaaacaaa ccagcgtttt tgcgccgaac gtgctgattc ttgatcagaa
     7381 catgacccca tcagccttct tccccagcag ttatttcacc taccaggaac caggcgtgat
     7441 gagtgcagat cggctggaag gcgttatgcg cctgacaccg gcgttggggc agcaaaaact
     7501 ttatgttctg gtctttacca cggaaaaaga tctccagcag acgacccaac tgctcgaccc
     7561 ggctaaagcc tatgccaaag gcgtcggtaa ctcgatcccg gatattcccg atccggttgc
     7621 tcgtcatacc agcgatggct tactgaaact gaaagtcaaa acgaactcca gctccagcgt
     7681 gttggtagga ccactatttg gttcttccgc tccagctccg gttacggtag gcaacacggc
     7741 ggcaccagct gtggctgcac ctgctccggt gccggtgaag aaaagcgagc cgatgctcaa
     7801 cgacacggaa agttatttta ataccgcgat caaaaacgct gtcgcgaaag gtgatgttga
     7861 taaggcgtta aaactgcttg atgaagctga acgcctggga tcgacatctg cccgttccac
     7921 ctttatcagc agtgtaaaag gcaaggggta attacgcccc acagtgctga ttttgcaaca
     7981 actggtgcgt ctcctggcgc accttttttt atgcttcctt cctgggatat gagcgatttt
     8041 ttatagtact tcacttcttc ttcactaaga atgtccatta tctcaatgcc ttatcagaga
     8101 ttcttttcct ttcgccggta gtgtctggac gctcaggcta cttttccagg ttattttatt
     8161 tctgtattac agaggtttta tgataggtca tatcacaaat tctggcggca ataactcttt
     8221 gatgaaacat gatgtggttc aaggaaataa tatagtagat cttgatttac tacgtaattt
     8281 caatggggtg ac
//
LOCUS       DAZOYL010000174         8281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZOYL010000174 DAZOYL010000000
VERSION     DAZOYL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.175"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            85..1581
                     /gene="ravA"
                     /locus_tag="ACHUOY_002810"
     CDS             85..1581
                     /gene="ravA"
                     /locus_tag="ACHUOY_002810"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR0683543.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1575..3026
                     /gene="viaA"
                     /locus_tag="ACHUOY_002811"
     CDS             1575..3026
                     /gene="viaA"
                     /locus_tag="ACHUOY_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR0683544.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="ACHUOY_002812"
     CDS             complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="ACHUOY_002812"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR0683545.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            4175..4633
                     /gene="asnC"
                     /locus_tag="ACHUOY_002813"
     CDS             4175..4633
                     /gene="asnC"
                     /locus_tag="ACHUOY_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR0683546.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4723..5166
                     /gene="mioC"
                     /locus_tag="ACHUOY_002814"
     CDS             4723..5166
                     /gene="mioC"
                     /locus_tag="ACHUOY_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR0683547.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5545..7434
                     /gene="mnmG"
                     /locus_tag="ACHUOY_002815"
     CDS             5545..7434
                     /gene="mnmG"
                     /locus_tag="ACHUOY_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR0683548.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7498..8121
                     /gene="rsmG"
                     /locus_tag="ACHUOY_002816"
     CDS             7498..8121
                     /gene="rsmG"
                     /locus_tag="ACHUOY_002816"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR0683549.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2090 a         2105 c         2106 g         1980 t
ORIGIN      
        1 ctttgagact attttagcca ccagcaagaa acgtctatac tcgcaattta cgcagaactt
       61 ttgacgaaag gacgccactt cattatggct caccctcatt tattagcgga aagaatttcc
      121 cgcctgagca gttcgttgga aaaggggctt tatgaacgta gccacgccat ccgcttgtgt
      181 ttattagcgg cattaagtgg tgaaagtgtg ttcctccttg gcccgccagg tattgccaaa
      241 agtttgatcg cccggcgctt aaaattcgcc tttcagaatg cccgcgcgtt tgaatatctg
      301 atgacccgct tctccacgcc ggaagaagtt tttggtcccc tttctattca ggcgctaaaa
      361 gatgaagggc gctatgaacg tttaaccagc ggttacctgc cggaagcaga aattgtcttt
      421 ctggatgaga tctggaaagc gggcccggca attcttaata ccttgctcac cgccattaac
      481 gagcgccagt tccgcaacgg tgcacacgta gaaaaaatcc cgatgcgtct gctggtggcg
      541 gcctccaacg agctgccgga agcagacagc agtctggaag cgttatatga ccgcatgctg
      601 attcgtctgt ggttagataa agtgcaggat aaagcgaatt tccgctccat gctgaccagt
      661 caacaggatg aaaacgacaa tccggttcct gatgccttgc aggtcacaga tgaagaatat
      721 gaacgctggc agaaagagat tggtgaaatt acgctgcccg atcatgtatt tgagctgatt
      781 tttatgctgc gcctgcaact ggataaatta ccggatgcgc cttatgtctc ggatcgtcgc
      841 tggaaaaaag cgatccgatt attgcaggcc agcgcctttt ttagcggtcg cagtgctgtt
      901 gccccggtag atctcatttt gctgaaagat tgcctgtggt atgacgcgca aagcctgaat
      961 ttgatacaac aacaaattga tgtattgatg accggtcatg cctggcaaca gcaagggatg
     1021 ttgactcgcc ttggcgcgat tgtgcaacgt cacctgcaac tccagcagca acaaagcgat
     1081 aaaacagcct taacggtaat tcgtctgggc ggcattttca gccgtcgtca gcagtatcaa
     1141 cttcctgtta acgttactgc ttccactctg actctactgc tgcaaaaacc gttaaaactg
     1201 catgatatgg aagtggttca tatctccttt gagcgtagcg cgctggaaca gtggctgagc
     1261 aaaggtggtg aaattcgcgg taaactgaac ggtatcggct ttgcccagaa actgaatctg
     1321 gaagttgata gcgcccaaca tcttgtcgtt cgcgatgtaa gtttacaagg cagtacgctg
     1381 gcacttcccg gttcatcggc tgaaggtctg cctggtgaaa taaaacaaca actggaagag
     1441 cttgaaagcg actggcgcaa gcaacacgct ttattcagcg aacagcaaaa atgtctgttt
     1501 atccctggcg actggttagg tcgcattgaa gccagcctac aggatgtcgg tgcacagatt
     1561 caccaggcac aacaatgcta acgctggata cgcttaatgt gatgctggcc gtcagcgaag
     1621 agggattgat cgaagagatg atcatcgcac tgctggcctc accgcagctg gcggtcttct
     1681 ttgaaaaatt cccacgcctg aaggcggcaa tcactgatga tgttccccgc tggcgtgagg
     1741 cgctgcgcag tcggctgaaa gatgcccgag tcccgccgga actcaccgaa gaggtgatgt
     1801 gctatcagca aagccagctc ctctccacgc cgcagtttat tgtgcagcta ccacagatcc
     1861 tggacttact gcatcgtctg aattccccat gggcagaaca agcccggcag ttggttgatg
     1921 ctaacagcac aataacttca gcgttacaca cgctttttct ccagcgttgg cgtttaagtc
     1981 tgatcgtgca agcaacgacg ttaaatcaac agctattaga agaagaacgc gaacaactgt
     2041 tgagtgaagt tcaagaacgc atgacgctga gcggacaact tgaaccgatt ctcgcagata
     2101 acaatactgc agctggtcgt ctgtgggata tgagcgccgg tcagcttaaa cgtggcgact
     2161 atcagttgat tgtgaaatac ggtgaatttc ttaacgaaca gccggaactg aaacgcctgg
     2221 cagagcagct ggggcgttct cgggaagcca aatcaatacc gcgcaacgat gcgcagatgg
     2281 aaaccttccg caccatggtg cgcgaaccgg cgacggttcc tgagcaggtt gatggtctgc
     2341 aacaaagcga tgatatttta cgtcttctgc cgccagaact ggcgacacta gggataacgg
     2401 aactggagta tgagttttac cgtcggctgg tggaaaaaca gttgctcacc tatcgcctgc
     2461 acggtgagtc gtggcgtgaa aaagtgatcg aacgtccggt ggtacataaa gattacgatg
     2521 aacagccgcg cgggccgttt attgtctgtg tggatacttc cggctcaatg ggcggcttta
     2581 atgaacagtg tgcgaaagcg ttctgcctgg ccttgatgcg cattgctctc gctgaaaacc
     2641 ggcgctgcta tattatgcta ttttccaccg agatcgtccg ttatgagctt tcaggccccc
     2701 aaggcatcga acaagcaatc cgttttttaa gccagcagtt tcgtggcggc accgatcttg
     2761 ccagttgttt tcgcgccatt atggaacgct tgcaaagcag ggaatggttt gatgccgatg
     2821 cggtggtgat ttctgatttt atcgctcagc ggttgcctga cgacgtgacg agtaaagtga
     2881 aagagctgca gcgggtacat cagcatcgct ttcatgccgt ggcgatgtcg gcacacggca
     2941 aacccggcat catgcgcatt ttcgatcata tctggcgctt tgataccggg atgcgaagcc
     3001 gcctgctcag acgctggcgg cgataaatta ttacagcaga gaagggacgt tctcgcgaac
     3061 agcagctggc catacaccac actgaacctg gccgatatgc ggcagttgca gcagcagcat
     3121 ggtcaaacga gactggccga taccgccgcc gatggtctgc ggcatttcac cgcgcagcag
     3181 cgcctgatgc cattccagct gcagacgatc ttcatcaccg gtcagcgcca actgatgctt
     3241 caacgtgtcg gcatccacgc ggatccccat ggaagaaagc tcaaacgcat cttccagtac
     3301 agggttccac accagaatat cgccgttcag acccgcatga cccagctctg acggggtgct
     3361 ccagtcatca taatccggtg cgcgcacgtc gtggcgatga ccatcgctca gcttgccgcc
     3421 aatcccgaca aggaataccg cgccaagatc tttcgctatc gcccgctcac gccctttggc
     3481 atcgagatcc ggataacgag acagtaactc ctggctgtgt acgaagtgga tctgatccgg
     3541 caggaacggt gccaggccaa actcttcgct aaccgcagct tcggttgctt taattcccgc
     3601 ccagatcgcc tctaccgtgc ttttcagagt cgagaattga cgctcaccgt cgcccattac
     3661 gcgttcccag tcccactggt caacatagac cgagtgcaac ggagaaagac ggtcttcatc
     3721 ggggcgaagg gctttcatgt gcgtgtacag cccttcgccc gcgctgaagt cgtgttgccc
     3781 taaggtctga cgtttccact tcgccagtga atgaaccact tcgaactggg catcaggcag
     3841 agctttcact tttacctgca ccgctttttc acagcccgac aagttatcct gcgtgccatc
     3901 ccccacacgg ctaagaatcg gcgcctggac ttcgatcagc cccagacgtt cttccagttg
     3961 gcgagaaaag tgagatttca cgaagctaat ttggcgttgt ttggcaatgt aagcggtttt
     4021 cattttttat actcctgcgt cctgttgctt atgattaagc aacaaaaacc gacaaacatg
     4081 caataatcat tcaataaaaa gcctaacaca cttttgattc attaaaaaag aaggctaaaa
     4141 tagaatgaat catcaatctg cataagaaaa tcctatggaa aattatctga tcgacaatct
     4201 ggaccgtggc atcctggaag cattaatggg caatgcgcgc accgcttacg ccgaactggc
     4261 gaaacaattt ggcgtcagtc cggggacgat tcacgttcga gtagagaaaa tgaagcaggc
     4321 ggggatcatt accggggcgc gtattgatgt cagcccgaag cagctcggtt atgacgtagg
     4381 ctgctttatc ggcattatat taaagagcgc caaagactac ccttccgcgc tggcaaagct
     4441 ggaaagcctt gctgaagtca ctgaagccta ttacacaaca ggccactaca gcatctttat
     4501 aaaagtaatg tgccgttcga tcgacgctct ccagcatgta cttatcaaca agatccaaac
     4561 aattgatgaa attcagtcca ccgagacatt gatcgtcctg caaaacccga tcatgcgtac
     4621 catcaagcca taatcggctt ttttaatccc atacttttcc acaggtagat cccaacgcgt
     4681 tcacagcgta caatacgcca ctcttaataa aggtggcggt ttatggcaga tatcactctt
     4741 atcagcggca gcaccctcgg cggtgccgaa tatgtagcag aacacctggc tgaaaagctg
     4801 gaagaggcgg gttttaccac cgaaacgctg cacggtccgc tgttggaaga tttatctgcc
     4861 tcggggatct ggctgattat cagctccacc cacggtgccg gagatattcc ggacaacctt
     4921 tctcctttct atgaagcatt gcaggaacag aagcccgatc tttctgcggt ccgctttggc
     4981 gcaatcggta ttggtagtcg tgaatatgac accttttgtg gggctatcga taaactcgag
     5041 gcagaactca aaaattccgg tgcaaaacag acaggcgaaa cactgaagat caacattctt
     5101 gatcacgaca ttccggaaga tccggcagaa gaatggctgg gatcgtggat taatttactc
     5161 aaataagtat acagatcgtg cgatctactg tggataactc tgtcaggaag cttggatcaa
     5221 ccggtagtta tccaaggaac aacggttgtt cagtttttga gttgtgtata acccctcatt
     5281 ctgatcccag cttatacggt ccaggatcac cgatcattca cagttaatga tcctttccag
     5341 gttgttgatc ttaaaagccg gatccttgtt atccacaggg tagtgcgatc ctaataagag
     5401 atcacaatag aacagatctc taaataaata gatcttcttt ttaataccca ggatcccagg
     5461 tctttctcaa gccgacaaag ttgagtagaa tccacggccc gggcttcaat ccattttcat
     5521 accgcgttat gcgaggcaat caccatgttt tatccggatc cttttgacgt catcatcatt
     5581 ggcgggggtc atgcaggcac cgaggccgcg atggccgcgg cgcgtatggg tcaacagact
     5641 ctgcttttga cacacaatat cgacactctg gggcagatga gctgcaaccc ggcgatcggc
     5701 ggtattggga agggacatct ggtaaaagaa gtggatgcac tcggcggtct gatggcgaaa
     5761 gcgatcgatc aagcgggtat ccagtttagg atactaaacg caagcaaagg accggcggtt
     5821 cgcgctaccc gagctcaggc ggatcgtgtg ctctaccgtc aggcggtacg tacggcgctg
     5881 gagaaccaac cgaacctgat gatcttccag caggcggttg aagatcttat tgtcgaaaac
     5941 gatcgcgtgg tcggtgctgt tacccaaatg ggactgaagt tccgtgccaa agccgtcgtg
     6001 ctcaccgttg ggacgttcct cgacggtaaa attcatatcg gtctggataa ttacagcggt
     6061 ggtcgtgctg gtgatccgcc gtccattccg ctttctcgcc gtttgcgtga actgccgctg
     6121 cgcgttggtc gtctgaaaac cgggacacca ccgcgtattg atgctcgaac tatcgacttt
     6181 agtgtgctgg cgcaacagca tggcgataac ccaatgccgg tattctcgtt tatgggcaat
     6241 gcgtcccagc atccccagca ggtgccgtgt tatatcactc ataccaacga gaaaacccat
     6301 gatgtgatcc gcagtaacct cgatcgtagc ccaatgtacg caggggtgat cgaaggtgtc
     6361 ggcccacgct actgcccatc gatcgaagac aaagtcatgc gcttcgccga cagaaatcag
     6421 catcagatct tccttgaacc ggaagggctg acctctaacg aaatttatcc gaacggtatc
     6481 tccaccagcc tgccgttcga tgtgcagatg caaatcgtcc gctccatgca ggggatggaa
     6541 aacgcgaaga tcgtgcgtcc gggttatgcc attgagtatg acttcttcga tccacgcgac
     6601 ctgaaaccga cgctggagag caagtttatc caggggctgt tctttgctgg tcagattaac
     6661 ggcactaccg gttacgaaga agccgctgcg caaggtttgc tggtcggtct taacgctgcc
     6721 cgtctgtctg ctgacaaaga aggttgggct ccggcgcgtt ctcaggcgta tctcggcgta
     6781 ctggttgatg acctgtgcac tttaggaacc aaagaaccgt atcgtatgtt tacttcgcgc
     6841 gcagaatatc gtctgatgct gcgcgaagat aatgcggatc tgcgtttgac tgaaatcggt
     6901 cgtgaactgg gcctggtgga tgacgaacgt tgggcgcgct ttaacgagaa acttgagaat
     6961 atcgagcgtg agcgtcagcg tctgaaatcg acctgggtaa ccccgtcggc ggaagctgca
     7021 gccgaagtga atgctcacct gactgcgccg ctttcccgtg aagccagtgg tgaagatctg
     7081 ctgcgtcgtc cggaaatgac ttatgaaaaa ttaaccacgc tgacgccgtt tgcccctgcg
     7141 ttgacagacg aacaggcggc ggaacaggtt gagattcagg ttaaatacga aggttatatc
     7201 gcgcgccagc aagatgagat cgaaaagcag ctgcgtaacg agaacaccct gctacccgcg
     7261 acactggatt accgccaggt atccggtctt tctaacgaag tgatcgccaa acttaacgat
     7321 cacaaaccgg cctctatcgg ccaggcttcg cgtatttctg gcgtcacgcc tgcggctatc
     7381 tccattctgc tggtgtggct gaaaaaacag ggtatgctgc gtcgtagcgc ataacgcatt
     7441 aaaaatgcct ggtaagcacc cgcttaccag gcaacgcatc aagaacaggt aatcaccgtg
     7501 ctcaacaaac tctccttact gctgaaagac gcaggtattt cgcttaccga tcaccagaaa
     7561 aaccagctta ttgcctacgt gaatatgctg cataaatgga acaaagcgta caacctgact
     7621 tcggtccgcg atcctaatga gatgctggta cgccatattc tcgatagcat tgtggtggca
     7681 ccgtatctgc aaggtgaacg gtttatcgat gtcggcaccg gaccaggact gccaggcatt
     7741 ccactctcta tcgtgcgtcc tgaagcccat ttcactctgt tggatagcct tggtaaacgc
     7801 gtgcgtttcc ttcgtcaggt gcaacatgag cttaaactgg agaatattga gccagtacag
     7861 agcagggtag aagagtttcc ttcagagccg ccatttgatg gcgtaattag ccgcgctttt
     7921 gcctctctga acgatatggt gagctggtgc caccatcttc ctggtgagca aggccgtttc
     7981 tacgcgctga aagggcaaat gccggaagat gaaatcgctt tgttgcccga agaatatcag
     8041 gtcgaatcag tggttaaact tcaggttcca gctctggatg gcgaacgtca tctggtggtg
     8101 attaaagcaa ataaaattta atttttatca aaaaaatcat aaaaaattga ccggttagac
     8161 tgttaacaac aaccaagttt tctactgata taactggtta catttaacgc cacgttcact
     8221 cttttgtatc aacaagataa cgtggctttt tttggtaagc agaaaataag tcattagtga
     8281 a
//
LOCUS       DAZOYL010000175         8227 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZOYL010000175 DAZOYL010000000
VERSION     DAZOYL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.176"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(359..1555)
                     /gene="hemY"
                     /locus_tag="ACHUOY_002817"
     CDS             complement(359..1555)
                     /gene="hemY"
                     /locus_tag="ACHUOY_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR0683550.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1558..2805)
                     /gene="hemX"
                     /locus_tag="ACHUOY_002818"
     CDS             complement(1558..2805)
                     /gene="hemX"
                     /locus_tag="ACHUOY_002818"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR0683551.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPQGDTPAAAPQGE"
     gene            complement(2827..3567)
                     /gene="hemD"
                     /locus_tag="ACHUOY_002819"
     CDS             complement(2827..3567)
                     /gene="hemD"
                     /locus_tag="ACHUOY_002819"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR0683552.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(3564..4505)
                     /gene="hemC"
                     /locus_tag="ACHUOY_002820"
     CDS             complement(3564..4505)
                     /gene="hemC"
                     /locus_tag="ACHUOY_002820"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR0683553.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4892..7438
                     /gene="cyaA"
                     /locus_tag="ACHUOY_002821"
     CDS             4892..7438
                     /gene="cyaA"
                     /locus_tag="ACHUOY_002821"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR0683554.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7478..7798)
                     /gene="cyaY"
                     /locus_tag="ACHUOY_002822"
     CDS             complement(7478..7798)
                     /gene="cyaY"
                     /locus_tag="ACHUOY_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR0683555.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7960..8102)
                     /locus_tag="ACHUOY_002823"
                     /pseudo
     CDS             complement(7960..8102)
                     /locus_tag="ACHUOY_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
BASE COUNT         1830 a         2174 c         2150 g         2073 t
ORIGIN      
        1 agctcaggtt aaatattcac tcaggaagca aagaggatta cagaattatc tcataacaag
       61 tgttaaggga tgttatttcc cgattctctg tggcataata aacgggtaga tgctcattcc
      121 atctcttatg ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt
      181 gcttatcgtc cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg
      241 agtgaagcac ccactgttgt catacagacc tgttttaacg cctgctccgt aataagagca
      301 ggcgtttttt tatgcatcag aaaggtcccg gaggtgcttg cctccgggtg agaaggaact
      361 actgtggtgg gttgttctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt
      421 cttccggctt gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg
      481 gacgttgttt cagcgctgcg cggaaggcga gcgatgcttc ctgccattct ccgtgcttca
      541 tcagtgactg gcccagtgtg ctccacaaca gcgggcgatc gccgacgttt ttgatctgct
      601 ggcgcagcac tttttccagc tgttccggat tgttggtttt cagtcgagga atcggcagca
      661 gtaggcgatc gtcgtactgg cgtttcaggc catcgatgat aatttgctgg gcagtatcat
      721 gatcgtcaca ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg
      781 ttttccggct ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg
      841 cctgatccat caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac
      901 caacatgggc tttcgccatt gatgggatga tatccagcag agaactccat gcacctgtgc
      961 ggatatacgc ctgttccgcc agacgtaata cttccggatg gcgtggcgta acttccagca
     1021 gcttatccac gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac
     1081 gggtgatttc taccggaatg gtgtcattgc cggccagctc tgctgcgcgt tccagatgtt
     1141 ggttggcgcg tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca
     1201 ccaccggttg ttccgcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat
     1261 cgccttccgc cagtttcagc agcgcctgtt cggtctgctt acgggcacgg cgacgcttac
     1321 gtccgacaaa ccacccacgg gtgtgtgcgc ctgtgcggaa gatccgccgc agtagccact
     1381 caatggcaaa cagcactacc atcgccagaa tcaaaataat cgccaggccc gtgacgctgg
     1441 tttcgatatt gtagttgtca gtctggatca gcacgtaacc ctgatgaccg gcaatcatcg
     1501 ggccaaccac tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta
     1561 ttctccttgc ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc
     1621 aggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggttt
     1681 agcttccgtt gtccccgctg ccggttgtgc cagcaggtta cgcacgcggg tctgcatcag
     1741 tttttccagc atcgcctggc tttgcagggt ttccggaaga tccatcgaga tattttgctg
     1801 gcttaactgg tccacctcgt cgaggaacgc tttggtggtg gcatcatcag tatcgtagta
     1861 agcacgtacc caggtggaga cgttctccag cgcctggcga taagtctctt cctgatgacg
     1921 cggtacagct tgtgctgcga ccagcaggcg agagcgaata ttttcgcgca gatagatatc
     1981 ctgatttggc gctaacagcg gtacggcggt gtcatcacga cggcgaatcg taatgaagtt
     2041 gtccataaag ttctgccagc ttttttgcag attgatacgc cattcgctga tggaactgga
     2101 aagctcttca ccgtctgaat ccatcggcga accatcgctg tcattatcgg ccagacgcag
     2161 gttatctacc tgatttgaaa gctgattaag cttaaggatg atgccgtcat aatccacctg
     2221 cgatactgca gaaaggctgg cgatatcatc ggtaattgcc cgacgaacgg taatcagact
     2281 cgggtcattc atatccgcca ggctggcgtc tgcacttttc agcaacgctg cagcggtcgt
     2341 gacgtcctga tcgctccaca gcttccgtcc ggcgagtttc accagaaaat cggcctgggc
     2401 cagcagccag gttttagcat cgctgccgga aatggtggcg accttttgct ggacttcatc
     2461 cagctgtttt gccagcgttt cttgctgacg attcgcctgt tcaagctgcg tagcctgttg
     2521 cttaataatg ccttccagtt cggctttttg gctctcctgg gctttttgca atgccgtcag
     2581 ttggttagcc agggcatcgc tggtggcggt ctgattgacg gcctgttgtt taccccagcc
     2641 atacaaaccg acgcccgccg ccagagcaat agcgatagcc gccgcgctga gaatcaatgc
     2701 ggtattgttc ttactctttt tttctgttgc gacaggttgt gacgtggtgt ccacggcctc
     2761 cctagtctct tcaaccacgg cggaggtttt ttcttgttcc gtcattatgg cttcctgtta
     2821 tgagagttat tgtaatgccc gtaaaagcgc atcgttggca gcgttatcgg cgaccttaat
     2881 gtcttgccag cccagttccc gggcgagttt cgccaaacgc tcactgacga ccaatagtcg
     2941 acagtgtagt aaccagtgct cacgatacca ttgtgggatc agcgaccaga gttgctgcaa
     3001 catttcaccg ctggtaacaa cgaccgtcgt cacctcgcgg gattgccagc gcatcgcttc
     3061 ttctgcacca tcgtaatgga ttgcgcatcg ttgataacat tcacaaaaag tgacctcagc
     3121 accgcgcgcc gtcagggtat ccccaattag ctcacgaccg ccattgccac gtaatatcag
     3181 cgcacgtttg cccgcaatat tttgtaattc aggtaattgt agcaagactt cgctgatttc
     3241 ccgatcctgc gggtagagaa tcttctgtcc gcttacggta tgtagtgcca gtgcggtggt
     3301 gcgtccaatg gcgaaataat taggtagtcg gggccattta cgatcttgct gatgtagctg
     3361 tgattgggca aaagcaaccg cgtgttgcga gagggcaaac aacagatcgc tctcccccag
     3421 cgctgccagt tgatcagcaa gttgcggtaa ttgttgaccc ggagaaaact caatcagcgg
     3481 aaaatgccag gccacctgcc ccagtgtgcg cagacggctc actaactctt ctccagcggg
     3541 agacgggcgg gtgacaagga tactcatgcc ggggcgtctc cgttatagac ttcagcgagg
     3601 atctcgcgcg cgccgttatt cagtagctct tctgccagcg aaatccccat ttgtttggca
     3661 ttttgcggcg caccgcggcg ttcaccgcga ataatctgcg aaccgtccgg cgcgccgacc
     3721 agcgcacgca gccagatttc gccatcaata agctcggcgt agctaccaat tggcacctga
     3781 catccgcctt cgagacgggt attcatggcg cgttctgcgg taacgcgcag tgcagtttcg
     3841 tggtgattca gcgcggcaag cagctcgcga gtgcgcgtat catcaaggcg gcattcaata
     3901 ccgactgcac cttgtcctac cgccggaaga gaaatctcgg gtggtaacgc ggcgcgaatg
     3961 cgggactcca gacctaaacg ttttagtccg gctacggcaa gaatgatggc atcgtattcg
     4021 ccgttatcta gtttgctcag gcgagtgccg acgttgccgc gcagggagcg gataatcaga
     4081 tccgggcggc gttcagccag ttggcactgg cgacgtaaac tggacgtccc gacgatactg
     4141 cctgctggta acgcatccag actgtcatag ttattggaca caaaggcatc gcgaggatct
     4201 tcacgctcac aaatagtgac cagtcccaga ccttgcggga attcaaccgg cacatctttc
     4261 attgaatgta cggcgatatc ggcgcgattt tcgaggagcg cgacttccag ctcttttaca
     4321 aataagcctt ttccgcctac tttcgccagc ggcgtatcaa gaatcacatc gccgcgcgtc
     4381 accatcggta ccagttcaac gaccaggccc ggatggctcg ccatcaactt gtctttgaca
     4441 tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg caattcttaa aacattgtct
     4501 aacatgcttg ttaccgtcat tatcatccgt ggtccatcct aacatccttg ccagagtgat
     4561 gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt aaaatgaggt ctggcagtgg
     4621 atcctgacag acgtttcacg ctgtcgtaat aaggaattta cagagaataa acggtgctac
     4681 acttgtatgt agcgcatctt tctttacggt caatcagcaa ggtgttaaat tgatcacgtt
     4741 ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg cgaaaagtgg taacggttac
     4801 ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt tgatgttggc ggaatcacag
     4861 tcatgacggg tagcaaatca ggcgatacgt cttgtacctc tatattgaga ctctgaaaca
     4921 gagactggat gccataaatc aattgcgtgt ggatcgcgcg cttgctgcta tggggcctgc
     4981 attccaacag gtctacagtc tactgccgac attgttgcac tatcaccatc cgctaatgcc
     5041 gggttacctt gatggtaacg ttcccaaagg catttgcctt tacacgcctg atgaaactca
     5101 acgccactac ctgaacgagc ttgaactgta tcgtggaatg tcagtacagg atccaccgaa
     5161 aggtgagctt ccaattactg gtgtatacac catgggcagc acctcgtccg tagggcaaag
     5221 ttgttcctct gacctggata tctgggtctg tcatcaatcc tggctcgata gcgaagagcg
     5281 ccagttacta caacgtaaat gcagcctgct ggaaagctgg gccgcctcgc tgggtgtgga
     5341 agtcagcttc ttcctgattg atgaaaaccg ctttcgtcat aatgaaagcg gcagcctggg
     5401 gggcgaagat tgtggctcca cccagcatat actgctgctt gacgaatttt atcgtaccgc
     5461 cgtgcgtctc gccggtaagc gtattctgtg gaatatggtg ccgtgcgacg aagaagagca
     5521 ttacgacgac tatgtgatga cgctttacgc gcagggcgtg ctgacgccaa atgaatggct
     5581 ggatctcggt ggcttaagct cgctttctgc tgaagagtac tttggtgcca gcctttggca
     5641 gctctacaag agtatcgatt ccccatacaa agcggtgctg aaaacactgc tgctggaagc
     5701 ctattcctgg gaatacccga acccacgtct gctggcgaaa gatatcaaac agcgtttgca
     5761 cgacggcgag attgtatcgt ttggcctcga tccatactgc atgatgctgg agcgtgttac
     5821 tgaatacctg acggcgattg aagatttcac ccgtctggat ttagtacgtc gctgcttcta
     5881 tttaaaagtg tgcgaaaaac tcagccgtga acgcgcctgt gtaggctggc gtcgcgcagt
     5941 gttgagccag ttagtgagcg agtggggttg ggacgaagct cgtctggcaa tgctcgataa
     6001 ccgtgctaac tggaagattg atcaggtgcg tgaggcgcac aacgagttgc tcgacgcgat
     6061 gatgcagagc taccgtaatc tgatccgctt tgcgcgtcgc aataacctta gcgtctccgc
     6121 cagtccgcag gatatcggcg tgctgacgcg taagctgtat gccgcgtttg aagcattacc
     6181 aggtaaagtg acgctggtaa acccgcagat ttcacccgat ctctcggaac cgaatctgac
     6241 ctttatttat gtgccgccgg gccgggctaa ccgttcaggt tggtatctgt ataaccgcgc
     6301 gccaaatatt gagtcgatca tcagccatca gccgctggaa tataaccgtt acctgaataa
     6361 actggtggcg tgggcatggt ttaacggtct gctgacctcg cgcacccgtt tgtatattaa
     6421 aggtaacggc attgtcgatt tgcctaagtt gcaggagatg gtcgccgacg tgtcgcacca
     6481 tttcccgctg cgcttacctg caccgacacc gaaggcgctc tacagcccgt gtgagatccg
     6541 ccatctggcg attatcgtta acctggaata tgacccgaca gcggcgttcc gcaatcaggt
     6601 ggtgcatttt gatttccgta agctggatgt cttcagcttt ggcgagaatc aaaattgcct
     6661 ggtaggtagc gttgacctgc tgtaccgcaa ctcgtggaac gaagtgcgta cgctgcactt
     6721 caacggcgag caatcgatga tcgaagccct gaaaactatt ctcggcaaaa tgcatcagga
     6781 cgccgcaccg ccagatagcg tggaagtctt ctgttatagc cagcatctgc gcgggttaat
     6841 ccgtactcgc gtgcagcaac tggtttctga gtgtattgaa ctgcgccttt ccagcacccg
     6901 ccaggaaacc gggcgtttca aggcgctgcg cgtttctggt caaacctggg gcttgttctt
     6961 cgaacgcctg aatgtatcgg tacagaagct ggagaacgcc atcgagtttt atggcgctat
     7021 ttcgcataac aaactgcacg gcctgtcagt gcaggttgaa accaatcacg tcaaattacc
     7081 ggcggtggtg gacggctttg ctagtgaagg gatcatccag ttcttcttcg aagaaacgca
     7141 agacgagaat ggctttaata tctacattct tgacgaaagc aaccgggttg aggtgtatca
     7201 ccactgcgaa ggcagcaaag aggaactggt gcgtgacgtc agtcgcttct actcgtcatc
     7261 gcacgaccgc ttcacctacg gctcaagctt catcaacttc aacctgccgc agttctatca
     7321 gattgtgaag gttgatggtc gtgaacaggt gattccgttc cgcacaaaat ctatcggtaa
     7381 catgccgcct gccaatcagg atcacgatac gccgctatta cagcaatatt tttcgtgatg
     7441 aacgtgccgg aaagcgaggc ttatccggca tgcaatctta gcggaaactg actgtttcac
     7501 ccgcctgctg cgtcgccgcc tgttccagca aatcccagaa ggtttcgccg ctgcgatcgc
     7561 aaatccactc atcgcctttc agatcaaaat ggtagccgcc ttgtttggtt gccagccata
     7621 cctggtgcag cggttcctgg cggttgataa tgattttgct gccgttttca aaggtaatgg
     7681 tcagcacgcc gccgttgatt tcgcagtcga tatcgctgtc gccatcccag tcgtccaggc
     7741 gttcttcaat ggtcagccag agttgatcag ccaggcgatg aaattcactg tcgttcattg
     7801 ttgtatcctg tttttaagtg atggcggcag tatagcggca tggggtcagg gcttcaaagt
     7861 ttgcacctct gcggctgcgt tccggcacga ttcatccgtc accggaataa tgatgtctct
     7921 gtgtagcgaa agatttgtct cttcattagg gcgcagttac accacgcctt tccctgtttc
     7981 tggtaaacat tatgatcagg tttaccgagc gagcatcctc acgctgacgg aactaaaaaa
     8041 gacaacaaac aaaccacatt gcgatagtgc atagagccat cctggcgcga ggtgccgatc
     8101 acgaaactac cagcaaaaca taaatcccca cgagtaagcg ttatactcgc agcatttcct
     8161 cacttttcag acttcataaa gagtcgctaa acgcttgctt ttacgtcttc tcctgcgatg
     8221 atagaaa
//
LOCUS       DAZOYL010000176         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZOYL010000176 DAZOYL010000000
VERSION     DAZOYL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.177"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUOY_002824"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUOY_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR0683556.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUOY_002825"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUOY_002825"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR0683557.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUOY_002826"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUOY_002826"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR0683558.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHUOY_002827"
     CDS             6679..>8182
                     /locus_tag="ACHUOY_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR0683559.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2029 c         2321 g         1853 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact tggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZOYL010000177         8158 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZOYL010000177 DAZOYL010000000
VERSION     DAZOYL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.178"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..450)
                     /locus_tag="ACHUOY_002828"
     CDS             complement(<1..450)
                     /locus_tag="ACHUOY_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0683560.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
     gene            complement(567..1613)
                     /gene="ansB"
                     /locus_tag="ACHUOY_002829"
     CDS             complement(567..1613)
                     /gene="ansB"
                     /locus_tag="ACHUOY_002829"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR0683561.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1789..2508)
                     /locus_tag="ACHUOY_002830"
     CDS             complement(1789..2508)
                     /locus_tag="ACHUOY_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR0683562.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2692..3018)
                     /locus_tag="ACHUOY_002831"
     CDS             complement(2692..3018)
                     /locus_tag="ACHUOY_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR0683563.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3018..3737)
                     /gene="trmB"
                     /locus_tag="ACHUOY_002832"
     CDS             complement(3018..3737)
                     /gene="trmB"
                     /locus_tag="ACHUOY_002832"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR0683564.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3898..4950
                     /gene="mutY"
                     /locus_tag="ACHUOY_002833"
     CDS             3898..4950
                     /gene="mutY"
                     /locus_tag="ACHUOY_002833"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR0683565.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4978..5253
                     /locus_tag="ACHUOY_002834"
     CDS             4978..5253
                     /locus_tag="ACHUOY_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR0683566.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5318..6397
                     /gene="mltC"
                     /locus_tag="ACHUOY_002835"
     CDS             5318..6397
                     /gene="mltC"
                     /locus_tag="ACHUOY_002835"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR0683567.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6599..7855
                     /gene="nupG"
                     /locus_tag="ACHUOY_002836"
     CDS             6599..7855
                     /gene="nupG"
                     /locus_tag="ACHUOY_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR0683568.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7904..>8158)
                     /locus_tag="ACHUOY_002837"
     CDS             complement(7904..>8158)
                     /locus_tag="ACHUOY_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0683569.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1923 a         2159 c         1987 g         2089 t
ORIGIN      
        1 ataattgaaa tcttcgctat tcacgatcag atatttatta ttcgttttga tatcgtcgac
       61 cacccgccca cgatcgctgg ctgccgcctg aaaagagtaa aagttcaccg accagccaga
      121 cgcgggcgtt cctttactgg ttaacatcgc cgagaatttt accggtttat ctttcgtcca
      181 ggtctgttcg agcagttcgt aatctgccaa ctgaccgacc caggtataga gatcgtcact
      241 ggaagagaca tcgccttccg ctgaccaggt ggcctgatta cccttagcat caagattttt
      301 taaggtgata gcatcaagct tgagaatgcc gtgatactgc tttttgaatt gatcttttaa
      361 gatattttca gttggcggtt cgccatctgc ccaggcatta ccagtcatca acataagcag
      421 tgccgtgcca acaatccatt gctttttcac ccgaaaaacc tcacgcggaa taatttattc
      481 cggtgattat cattagggta atgataattg gcaatggacg taagtctgaa gtgaaaaagc
      541 cccggcacga taccggggcg aggtgattag tactgattga agatctgctg gatctgctgc
      601 ggatctttgg tttgcgtcag agccagctgc agcaggacgc gcgctttttg cgggttcagc
      661 gtgccagagg cgacgaagcc gtatttcgca tcatccactt cagcatcctg agtggtagca
      721 cccgtcggta cgcgggaaga acgcactact gcagtgccgt ttttcgcggc ggttgccagg
      781 gtgtcgaaca cggatttata caggttacca ttacccacac cagcgctaac gatgccatca
      841 tagcccgcat ctaccagtgc tttagccgga agatcggatg cgttagcgta gttataaacg
      901 atgccgactt tcggcagctc attcagctta gagacatcga atggcgtatc gctggtgtgc
      961 ttacgtgccg gggtacgttg gtagtcaatc ttaccgttgt gaatgtatcc cagaggaccg
     1021 tagttaacag acttgaaggt cgctacgtcg gtggtgttgg ttttggtgac atcgcgaccg
     1081 tccagtacgg tgtcgttcat caccaccagc acgccacgat tagcggaggc tttatcagct
     1141 gcggtcacta ccgcgttata caggttgaat ggaccgtctg cgctcatgga cgtggacggg
     1201 cgcattgcgc cgaccatcac caccggtttg tcgcatttca ccgtcaggtc gaggaagtaa
     1261 gcggtttctt ccatcgtgtc ggtaccgtgg gtaatgacga agccgtcggt tttatcgcag
     1321 tcggtgttaa tttttttcgc cagtgtcagc cagacattat cgttcatgtc ctgggagccg
     1381 atattcacta cctgctcgcc tttaacgttc gcaatgtctt ttagttgcgg caccgcatta
     1441 accagatttt ctacgccaac tttacccgct gtgtagttag atttggttgc ggagtcacca
     1501 ccaccggcaa tggtcccgcc ggttgctaaa atggtgatat tgggtaatgc caatgctgca
     1561 ccactaaaac ccataaccag tgcggcaagt gccgtctttt tgaaaaactc catttcattc
     1621 ctccagttac gtgaacgcta cgcattatcc cttagctctg tatgggaaat ttgacgttaa
     1681 acaatttaca acgtgaatat attttggaga tctacaaagt tagaggcagg taacaaaacg
     1741 aagaattaaa cggcataaaa aagtattatg ccgtcttaaa atagaggatt attttaaatt
     1801 accgaccagg gctttgcggc tatcttccag agtcacaacg cggctacaaa catctttgcc
     1861 aaactgctgg aaatcttttt cctgcttttt ccactctgtt tggattgagg attgcagccc
     1921 ccccaggctt cccagcacat tctgtaatgg gttaccgccg cttttcagca ccgctttcgc
     1981 gcccatttca ttaatgctgt cctgtaaaat tccacccatt gcctgattca ctaattgctg
     2041 gccttcggcg cgaacctgat caatggcttt atagtgaaac gtcaggccat cgctgcgcgt
     2101 ttcgataatg cggttcatct gctctttcag ctgcgcatca agtttggtca gacggctgcg
     2161 cattttgctg ctttcgccca tctcctgaac gataatttta tccagcgcaa tacgggcttt
     2221 ttcgacgcgg cttttcgcgc cttcatcaat ccacggcagc gtgctgcgta gttcagcctg
     2281 ataatccttc gcctgctcgc gctgggcggc attcagggaa tattgcttac cgttatacat
     2341 cacgttgccg tctggcgtga tcaccagatt gccgttttcg cccttcacct gcacggtttg
     2401 cgggctgaca atcacatcgt cacgcggcgt gacgctgcac tggtagtcgg cgtgagcggt
     2461 cattgccgtc actgaaagtg ctgccgccag cagcattttg cgcatcatag tcttccctca
     2521 agaaaaaatc aggccagcat ttgctggccc cagattgata acaaagtacg cgttttccat
     2581 gccggatgcg gcgtaaacac cttatcccgc ctactcgatc gtgcatcttt ccaggcctga
     2641 taagcgtagc gcatcaggca gttttgcatt tgtcatcgcc cgtatgcttt cttagtccca
     2701 ccaaacgtcg aaaagttcgc tggtgcgtac ctcatccagt ttgcgctctt ccagccactt
     2761 acgcacaatc gcctgatgtt cttcggtgca tttgccgatt tcctgcatgc agatcagacc
     2821 ttcccaggcc agataaccgc tgccgtcaaa ggccagtttg ttcggttcga taacctcgtt
     2881 aataaaatca tcaacggttt tatcaatctg ttcttccgat gtaccttccg ggaatcgcca
     2941 tgccaccgaa aatcctaatt cctggaattc gtcgatgtgc atttttttac gcagacgacg
     3001 gctacggttc tttgccatta tttcaccctc tcgaacatta agtcccatac tccgtgacca
     3061 agacgatgac cacgttgttc aaatttcgtc accggacgtg atgccggacg cggcacgtaa
     3121 tcattgctct ctgacaggtt tttataaccg tcaatagaag acatcacttc aagcatatgt
     3181 tccgcataag gttcccagtc ggtcgccata tggaatacac cccccagctg cagtttgctt
     3241 tttaccagtt cggcaaacgg cacctgtacg atacggcgtt tattatggcg cgctttgtgc
     3301 cacgggtcag ggaaaaagag ctgcaccatg cgcaatgaat tgtcaggaat cattttatgc
     3361 agcacttcaa ccgcatcgtg acacatcacg cgcaggttgc ttaaaccttc ttcatgcgca
     3421 gaagccaggc acgcaccaac gcccggtgaa tgcacttcaa tgccgaggaa gtcctgctca
     3481 gggcgatctt tagccattgc caccagcgac gcccccatgc caaaaccaat ctcaagcgtc
     3541 accggcgctt cacggccaaa aagcgcgggg aaatccagca tatcttcgct gaactcaacg
     3601 cccatcaccg gccagtagtt ttccagcgca tgttcctggc ctttggtcag tcgcccctgg
     3661 cggcgcacaa aactacggat acggcgcagt gggcggccgt tttcatcaaa ttccggtgaa
     3721 atgacgtcgt ttttcataaa gatttagtcg cttgtgaaag tgttctgaaa acgggcatta
     3781 tccaaagtta gttgccggat gcaagcatga taaggccgtg gctgcggaaa gttccggttt
     3841 acaccctgcc gtcgctgtgc tgcaatcttg cccccaacaa cagtgaattc ggtgaccatg
     3901 caagcgtcgc aattttcagc ccaggttctg gactggtacg ataaatacgg gcgaaaaacg
     3961 ctgccctggc aaattgacaa gacgccctac aaagtatggc tctcagaagt gatgttgcaa
     4021 caaactcagg ttgcgaccgt tatcccctat tttgaacgct ttatggcgcg cttcccgacg
     4081 gtgaccgatc tcgccaatgc gccgctcgac gaagttctcc acttgtggac cgggcttggc
     4141 tattacgccc gcgcgcgcaa tctgcataaa gcggcacaac aagtggcgac cttacacggc
     4201 ggtaaattcc cggaaacctt tgaagaagtc gcggcgttac cgggcgtcgg gcgttccacc
     4261 gcaggcgcga ttctctcgct ttctctgggt aagcactttc cgattctcga cggtaacgtc
     4321 aaacgcgtgc tggcgcgctg ctatgctgta agcggctggc ctgggaaaaa agaggtcgag
     4381 aataaattat ggagtttgag cgagcaggtg acgcccgcgg tcggcgtgga acggtttaat
     4441 caggcgatga tggatttggg cgcgatgatt tgtacgcgct cgaagccgaa atgttcgctc
     4501 tgtccgctac aaaacggatg tattgccgcc gccaataata gctggtcgct ttatccgggc
     4561 aaaaaaacga aacagacgct gccggagcgc accggctact ttttgctgtt acagcacgaa
     4621 gatgaagtat tgctggcgca gcgtccgccg agcggattgt ggggcggttt atactgtttc
     4681 ccgcagtttg ccgacgaaga aagtttgcgg cagtggctgg cgcaacggca gattgctgcc
     4741 gataacctga cgcaactgac cgcgtttcgg cataccttca gccatttcca cttagatatt
     4801 gtgcctatgt ggcttcccgt gtcgtcattc accggctgca tggatgaagg caatgcgctc
     4861 tggtataact tagcgcaacc gccgtcagtt ggcctggcgg ctcccgtgga gcgtttgtta
     4921 cagcagttac gcactggcgc gccggtttag cgcgtgagtc gataaagagg atgatttatg
     4981 agcagaacga ttttttgtac tttcctgcaa cgtgaagcag aaggtcagga ttttcagctg
     5041 taccccggcg agctgggaaa acgcatctat aacgagatct ccaaagaagc ctgggcgcag
     5101 tggcagcaca agcaaaccat gctgattaat gaaaagaaac tcaacatgat gaatgccgag
     5161 caccgcaagc tgcttgagca ggagatggtc aacttcctgt tcgagggtaa agaggtgcat
     5221 atcgagggct atacgccgga agataaaaaa taaaaacagt gccggagcac gcctccggca
     5281 acttgcataa aaacaaacac aacacgcacc cggaatgatg aaaaaatatc tcgcgctggc
     5341 tttgattgcg ccgttgctca tctcctgttc gacgaccaaa aaaggcgata cctataacga
     5401 agcctgggtc aaagatacca acggttttga tattctgatg gggcaatttg cccacaatat
     5461 tgagaacatc tggggattca aagaggtggt gatcgccggt cctaaggact acgtgaaata
     5521 caccgatcaa tatcagaccc gcagccacat caacttcgat gacggtacga ttactatcga
     5581 aactatcgcc gggacagaac ctgccgcgca tttgcgccgg gcaattatca aaacgttgct
     5641 gatgggtgac gatccgagtt cggtcgatct ctattccgac gttgatgata ttacgatttc
     5701 gaaagaacct ttcctttacg gtcaggtggt ggacaacacc gggcagccga ttcgctggga
     5761 aggtcgcgca agcaacttcg cggattatct gctgaaaaac cgtctgcaaa gccgcagtaa
     5821 cgggctgcgt atcatctaca gcgtcaccat taacatggta ccgaaccacc ttgataaacg
     5881 tgcgcacaaa tatctcggca tggtccgcca ggcgtcacgg aaatatggcg ttgatgagtc
     5941 gctgattctg gcaattatgc agaccgaatc ttcctttaac ccgtatgcgg tcagccgttc
     6001 cgatgcgctg ggattaatgc aggtggtaca acatactgcc gggaaagatg tgttccgctc
     6061 gcaggggaaa tccggcacgc cgagccgcag tttcttgttt gatcctgcca gcaatattga
     6121 taccggcacc gcgtatctgg cgatgctgaa caatgtttat ctcgacggaa ttgataaccc
     6181 aacatcgcgg cgttatgccg tcatcaccgc ctataacggc ggcgcaggca gcgtgctgcg
     6241 agtcttttcg aatgataaga ttcaggctgc caatattatt aacaccatga cgccgggcga
     6301 tgtttatcag acgctgacga cccgccatcc ctctgcggaa tctcgccgtt atctttataa
     6361 agtgaatacc gcgcaaaaat cctaccgccg ccgataattc cattaaccgc ccctgacgat
     6421 gctcaggggc aaaaatgtta tccacatcac aatttcgttt tgcaaattgg gaatgtttgc
     6481 aattatttgc cacaggtaac aaaaaaccag tccgcgaagt tgatagaatc ccatcatctc
     6541 gcacggtcaa atgtgctttt tcaaacactc atccgcatca caatgtgagg aaattaacat
     6601 gaatcttaag ctgcagctga aaatcctctc ttttctgcag ttctgtctgt ggggaagttg
     6661 gctgacgacc ctcggctcct atatgtttgt taccctgaag tttgacggtg cttctattgg
     6721 cgcagtttat agctcactgg gtatcgccgc ggtatttatg cctgcgctgc tggggattgt
     6781 ggccgacaaa tggttaagtg cgaaatgggt atatgccatt tgccacacca ttggcgctat
     6841 cacgctgttc atggcggcac aggtcacgac gccggaagcg atgttccttg tgatattgat
     6901 taactcgttt gcttatatgc caacgcttgg gttaatcaac accatctctt actatcgcct
     6961 gcaaaatgcc gggatggata tcgttactga cttcccgcca atccgtatct ggggcaccat
     7021 cggctttatc atggcaatgt gggtggtgag cctgtctggc ttcgaattaa gccacatgca
     7081 gctgtatatt ggcgcagcac tttccgccat tctggttctg tttaccctga ctctgccgca
     7141 tattccggtt gctaaacagc aagcgaatca gagctggaca accctgctgg gcctcgatgc
     7201 attcgcgctg tttaaaaaca agcgtatggc aatcttcttc atcttctcaa tgctgctggg
     7261 cgcggaactg cagattacca acatgttcgg taataccttc ctgcacagct tcgacaaaga
     7321 tccgatgttt gccagcagct tcatcgtgca gcatgcgtca atcatcatgt cgatttcgca
     7381 gatctctgaa accctgttca ttctgaccat cccgttcttc ttaagccgct acggtattaa
     7441 gaacgtaatg atgatcagta ttgtggcgtg gatcctgcgt tttgcgctgt ttgcttacgg
     7501 cgacccgact ccgttcggta ctgtactgct ggtactgtcg atgatcgttt acggttgcgc
     7561 attcgacttc ttcaacatct ctggttcggt gtttgtcgaa aaagaagtta gcccggcaat
     7621 tcgcgccagt gcacaaggga tgttcctgat gatgactaac ggcttcggct gtatcctcgg
     7681 cggcatcgtg agcggtaaag ttgttgagat gtacacccaa aacggcatta ccgactggca
     7741 gaccgtatgg ttgattttcg ctggttattc cgtggttctg gccttcgcgt tcatggcgat
     7801 gttcaaatat aaacacgttc gtgtcccgac aggcacacag acggttagcc actaattacg
     7861 caaagaaaaa cgggtcgcca gaaggtgacc cgttttttta ttcttacttc aacacataac
     7921 cgtacaatcg tttcatgcca tccgcatcgg tttcgctata gacaccttgc aactccggcg
     7981 aaaatcccgg caacaaattc acgccttctt ccagcgcaag gaaataacgt tgaaccgccc
     8041 caccccagac ttccccggga accacgcaaa gcacgccagg tggataaggc aacgcccctt
     8101 ctgccgcaat tcgcccttcg gcatcacgaa tccgcaccaa ctccacttca ccgcgaat
//
LOCUS       DAZOYL010000178         8125 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZOYL010000178 DAZOYL010000000
VERSION     DAZOYL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.179"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            321..437
                     /locus_tag="ACHUOY_002838"
                     /pseudo
     CDS             321..437
                     /locus_tag="ACHUOY_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            812..1054
                     /gene="yiiF"
                     /locus_tag="ACHUOY_002839"
     CDS             812..1054
                     /gene="yiiF"
                     /locus_tag="ACHUOY_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR0683570.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHUOY_002840"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHUOY_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR0683571.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHUOY_002841"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHUOY_002841"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0683572.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHUOY_002842"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHUOY_002842"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR0683573.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHUOY_002843"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHUOY_002843"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0683574.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHUOY_002844"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHUOY_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR0683575.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1892 a         2079 c         2095 g         2059 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tagtgacgtt ttcttttttc
     8041 atttattatg ttcactctct tatctatata tttataaggc aattaaatga aaaggaattt
     8101 attatcttcc gctattatag tcgcc
//
LOCUS       DAZOYL010000179         8123 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZOYL010000179 DAZOYL010000000
VERSION     DAZOYL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.180"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            163..317
                     /locus_tag="ACHUOY_002845"
                     /pseudo
     CDS             163..317
                     /locus_tag="ACHUOY_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            363..779
                     /gene="hicB"
                     /locus_tag="ACHUOY_002846"
     CDS             363..779
                     /gene="hicB"
                     /locus_tag="ACHUOY_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR0683576.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            858..2264
                     /locus_tag="ACHUOY_002847"
     CDS             858..2264
                     /locus_tag="ACHUOY_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFR0683577.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2509..3654
                     /gene="ydcS"
                     /locus_tag="ACHUOY_002848"
     CDS             2509..3654
                     /gene="ydcS"
                     /locus_tag="ACHUOY_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR0683578.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3672..4685
                     /locus_tag="ACHUOY_002849"
     CDS             3672..4685
                     /locus_tag="ACHUOY_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0683579.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4686..5586
                     /locus_tag="ACHUOY_002850"
                     /pseudo
     CDS             4686..5586
                     /locus_tag="ACHUOY_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5576..6370
                     /locus_tag="ACHUOY_002851"
     CDS             5576..6370
                     /locus_tag="ACHUOY_002851"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0683580.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6392..7816
                     /gene="patD"
                     /locus_tag="ACHUOY_002852"
     CDS             6392..7816
                     /gene="patD"
                     /locus_tag="ACHUOY_002852"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR0683581.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            8026..>8123
                     /locus_tag="ACHUOY_002853"
     CDS             8026..>8123
                     /locus_tag="ACHUOY_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HFR0683582.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         1919 a         1908 c         2222 g         2074 t
ORIGIN      
        1 ttcagttttg caaattagcg caaagaaatt ctggaatctt ccttcctgat tttgcattgc
       61 attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa ttacacatag gggtaaacac
      121 taatgtttac tttattgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc
      181 agacgttggc tcgaatctca gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc
      241 aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac
      301 aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa
      361 ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg
      421 tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa
      481 aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt atcccgttac
      541 cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaaag
      601 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
      661 ttggcaaacc taaacaggag attactcgcc tatttaactt gcaccatgcg acaaaaatcg
      721 acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat
      781 tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac
      841 ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
      901 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
      961 tttcaggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
     1021 atattatcgc acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga
     1081 aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg
     1141 atatgttgca ggccagtcgc gaaccgtcgg tcgttccgtt tgcctcggcc tttcccgacc
     1201 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
     1261 cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
     1321 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attactgccg
     1381 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
     1441 tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg
     1501 cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt
     1561 tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccactcggat
     1621 ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt actcaatcag tacaacgtaa
     1681 cgctgattga agatgacgtt tacagcgaac tttattttgg acgggaaaaa ccgctgcctg
     1741 cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc ttcgttttcg aaatgtctgg
     1801 tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct
     1861 tgcagttgat gagtacgctt tccaccagct caccgatgca acttgcgctg gtggattacc
     1921 tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt gcggaacgta
     1981 aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc ggaagttaaa attcatcata
     2041 gtgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgcc ggcgaattaa
     2101 gcctggcggc actgacgcat catatcagta ttgcgccggg taaaatgttt tctaccggtg
     2161 aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa cgtgaagaac
     2221 aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat
     2281 tcctcttaga ttgggtaata tgaatttcga atagcagtca tatttcctaa ccccttgtct
     2341 atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa
     2401 ttttctcagg gacgaaggtg tgcctgcaag ccgccgtcta tggttaaaca aggagatatt
     2461 tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca
     2521 tttgcccgca gcagcctgtg tgcgctcacc atgacaataa tgaccgctca cgccgccgaa
     2581 ccacctacca atttagataa accggaaggg cgactggata ttatcgcctg gccgggatac
     2641 atcgaacgcg gacaaactga taaacaatac gactgggtaa cgcaatttga aaaagagaca
     2701 ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg aaatggtcag tctgatgacc
     2761 aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggc
     2821 aaacgcgtgc agccgattaa taccgccttg attcccaact ggaaaacgct cgatccgcgc
     2881 gtggttaaag gcgactggtt taatgttggc ggcaaagttt acggcacacc ttaccaatgg
     2941 gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa
     3001 gtggtttttg ttgagcaaaa tctgccggac ggcaagagca ataaaggccg cgttcaggct
     3061 tatgatggcc ctatctacat tgcggacgct gcgttgttcg ttaaagccac tcagccgcag
     3121 ttgggcatca gcgatccgta tcaactaacc gaagaacagt accaggcggt gctgaaagtg
     3181 ctgcgcgctc aacacagttt gatccatcgc tactggcatg acactaccgt gcaaatgagc
     3241 gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc cctatcaggc caacgccctg
     3301 aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg agggtgttac cggttgggct
     3361 gataccacca tgctgcatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac
     3421 tggtcattaa cgccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccggta
     3481 gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa aaggttgtga aaccaacggt
     3541 tttaactatt tcgacaaaat cgccttctgg aaaacgccta tagcagaagg gggcaagttt
     3601 gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc
     3661 tggggtgctt tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg tacggtgacg
     3721 tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg
     3781 ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat tgctggcttc gaacagcttt
     3841 ccggcggggc tatctctatc tttggtaaac ccgccagcaa tctgccaccg tgggagcggg
     3901 acgtgaatac tgtctttcag gactacgcgc tatttccgca tatgtcgatt cttgacaatg
     3961 tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca gcggcacgca atggcgcaag
     4021 aggcgctgga gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg
     4081 gtggtcagcg ccagcgggtt gctatcgcca gagcattggt gaatgaaccg cgcgtattgc
     4141 tgttggatga accgctcggc gcactggatc tcaaattgcg tgagcagatg cagctggaac
     4201 tgaaaaaact gcaacagtct ctcggtatca cttttatctt cgttacccac gatcagggcg
     4261 aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt gagcaggtcg
     4321 attccccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgccggg ttcgttggta
     4381 catcgaatgt ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg
     4441 ccctgcgacc ggaacatatc cgcctcaaca cccctggtga actgcaggcc aatggcacga
     4501 tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga actgaaattg aacggcggtg
     4561 aaaaactgct tgtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc
     4621 ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg gttgaggaga
     4681 ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc aggtctgggg ctgtttttac
     4741 tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg ctgacactgt
     4801 tatggcaggg attttatact tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     4861 tggcgaatat ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     4921 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     4981 tggcgcgcta taccagcggg aaaatgaaag cgtttttata ttgcggtaat gttgccgatg
     5041 tgggcgagct acattgttaa agcctatgcc tggacgttat tgctggcaaa agatggcgtg
     5101 gctcagtggt ttttacaaca tcttgggctg gaaccactgc tgactgcgtt ccttacatta
     5161 cctgcggtgg gcggaaatac gctgtcaact tccgggctgg ggcgctttct ggtgtttctc
     5221 tatatctggt tgccgttcat gatcctgccc gttcaggcgg cgcttgagcg tttgccgccg
     5281 tcattgttgc aggcgtcggc tgatctcggc gcacgtccac gacaaacctt tcgctatgtg
     5341 gtgctgccgc tggcaatccc gggtattgcc gctggctcta tctttacctt ctcactcact
     5401 ctgggcgatt ttatcgtccc gcagctggtt ggtcctccag gatattttat cggcaatatg
     5461 gtttattccc agcagggggc gattggcaat atgccgatgg cggcggcatt caccttggtg
     5521 ccgattattc tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca
     5581 ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgttg ttttcctaca
     5641 ttttcccatc ctgataatcg ccgcctatgc gtttaacact gaagatgcgg cgtttagttt
     5701 tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct
     5761 tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaattg cgctggtgtt
     5821 agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc
     5881 gctgttactg ctgctgccca ttgcgctgcc gggcagcgtc actggtctgg cgttattaac
     5941 cgcctttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac
     6001 tttttgtgta gttgtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcctggag
     6061 tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc gctacgtagt
     6121 gttgccgaat ctcagttcgg cgttactggc aggaggaatg ctggcgtttg ccttgtcgtt
     6181 cgatgaaatc atcgttacga cctttacggc aggtcatgaa cgaacgttac tgttgtggtt
     6241 gctcaatcag cttgggcgac cgcgtgatgt accggtaact aacgtggtgg cactgctggt
     6301 tatgttggta acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg aaggcgacaa
     6361 tggtcaataa ccactgatac aggaatatgc tatgcaacat aagttactga ttaacggaga
     6421 actggttagc ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg gggacgtttt
     6481 actggaaatt gccgaggcat ccgcagagca ggtcgatgct gctgtgcgcg cggcagatgc
     6541 agcatttgcc gaatgggggc aaaccacgcc gaaagtgcgt gcggaatgtc tgctgaaact
     6601 ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc gtaattgtgg
     6661 caaaccgctg catagtgcgt tcaatgatga aatcccggcg attgtcgatg tttttcgctt
     6721 tttcgcgggt gcggcgcgct gtctgaatgg tctggcggca ggtgaatatc ttgaaggtca
     6781 tacttcgatg atccgtcgcg atccgttggg ggtcgtggct tctatcgcac cgtggaatta
     6841 tccgctgatg atggccgcgt ggaaacttgc tccggcgctg gcggcaggga actgcgtagt
     6901 gcttaaacca tcagaaatta ccccgctgac cgcgttgaag ttggcagagc tggcgaaaga
     6961 tatcttcccg gcaggcgtga ttaacatact gtttggcaga ggcaaaacgg tgggtgatcc
     7021 gctgaccggt catcccaaag tgcggatggt gtcgctgacg ggctctatcg ccaccggcga
     7081 gcacatcatc agccatgccg cgtcgtccat taagcgtact catatggaac ttggtggcaa
     7141 agcgccagtg attgtttttg atgatgcgga tattgaagca gtggtcgaag gtgtacgtac
     7201 atttggctat tacaatgctg gacaggattg tactgcggct tgtcggatct acgcgcaaaa
     7261 aggcatttac gatacgctgg tggaaaaact gggtgctgcg gtggcaacgt taaaatctgg
     7321 tgcgccggat gacgagtcta cggagcttgg acctttaagc tcgctggcgc atctcgaacg
     7381 cgtcagcaag gcagtagaag aggcgaaagc gacagggcac atcaaagtga tcactggcgg
     7441 tgaaaagcgc aagggtaatg gctattacta tgcgccgacg ctgctggctg gcgcattaca
     7501 ggacgatgcc atcgtgcaaa aagaggtatt tggtccagta gtgagtgtta cgcccttcga
     7561 caacgaagaa caggtggtga actgggcgaa tgacagccag tacggacttg catcttcggt
     7621 atggacgaaa gatgtgggca gggcgcatcg cgtcagcgca cggctgcaat atggttgtac
     7681 ctgggtcaat acccatttca tgctggtaag tgaaatgccg cacggtgggc agaaactttc
     7741 tggttacggc aaggatatgt cactttatgg gctggaggat tacaccgtcg tccgccacgt
     7801 catggttaaa cattaagtta gatgttagtg gctgttatag gacggccact atcgttttag
     7861 gttctgagtt taatggcggt taagtattag aatgattaca ttaataacta gcgtaaaaat
     7921 taggctaaat gtttgatggc gatttttcat cctgtcacag ttgattagca taaggcttta
     7981 gagtatcgtt attaaactaa taatttataa gtagaagtat taacaatgaa agttcaattg
     8041 tacaaattca cggaagataa aaataaaacc cttaccttca gatggacgaa aaaacacttt
     8101 gagttttgta tggataataa aat
//
LOCUS       DAZOYL010000180         8106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZOYL010000180 DAZOYL010000000
VERSION     DAZOYL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.181"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            175..234
                     /gene="ypfM"
                     /locus_tag="ACHUOY_002854"
     CDS             175..234
                     /gene="ypfM"
                     /locus_tag="ACHUOY_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR0683583.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(333..3446)
                     /gene="acrD"
                     /locus_tag="ACHUOY_002855"
     CDS             complement(333..3446)
                     /gene="acrD"
                     /locus_tag="ACHUOY_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR0683584.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(3610..5310)
                     /gene="narQ"
                     /locus_tag="ACHUOY_002856"
     CDS             complement(3610..5310)
                     /gene="narQ"
                     /locus_tag="ACHUOY_002856"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR0683585.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSPPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            5516..7495
                     /gene="aegA"
                     /locus_tag="ACHUOY_002857"
     CDS             5516..7495
                     /gene="aegA"
                     /locus_tag="ACHUOY_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR0683586.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            7563..>8106
                     /gene="nudK"
                     /locus_tag="ACHUOY_002858"
     CDS             7563..>8106
                     /gene="nudK"
                     /locus_tag="ACHUOY_002858"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR0683587.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLL"
BASE COUNT         1996 a         2127 c         2125 g         1858 t
ORIGIN      
        1 ccgtatgaat atctcgaaat gttgctgtgt atcacataca aatcatcacc gcaggaccat
       61 agtatttaca taagatttgt ccccccggag gtagtcatcg cagcaatcga gtaaattagc
      121 ttcaccacgg tatattgttt ccgtagaata cccataatta taagagaggt tgttatgatt
      181 gaacgtgaac tggggaactg gaaagacttt atcgaagtta tgcttcgtaa gtaattcctg
      241 agacaaagta taaacagcaa gaacccgctg agcaggttct taatcggaaa gttgaaaaag
      301 gcgacacatt ggcatgtcgc cttttttatt gcttattccg ggcgcggctt tagcgggaag
      361 cggcggcgca ccagcacaaa gaacagcggc acgaagtaaa tagccagaat agtggctgaa
      421 atcatcccgc ccattacgcc agtacccacc gcatgctgac caccggaacc ggcacccgtg
      481 ctggttgcca ttggcaatac gccgaagata aatgccagcg aggtcatcag aatcgggcgt
      541 aaacgctgac ggcaggcgtg gagcgtcgct tcaaacaggt cgtggccttt ttggttcatc
      601 tcgttagcaa actcgacgat caggatggcg tttttcgccg ataaaccaat gaccgttaac
      661 aggcccacct ggaagtaaac gtcgttttcc agcccgcgca tccaggttgc cagcagcgcg
      721 ccgattaccc ccagcggcac gaccagcatt accgagaacg gcaccgacca gctttcatac
      781 aacgcagcca gacacaggaa taccaccagc aaggaaatgg cgtacagcgc cggagcctgc
      841 gcgccggaaa gtcgctcctg atacgacatc gccgtccact ccagaccaaa gccgtttggc
      901 agctgcttca ctaacgattc cataatatcc atcgccgtac cggtactgac ccccggtgcg
      961 gcttccccaa caatctcaac cgcagaataa ccgttatagc gttccagacg cggcgagcct
     1021 gtttcccagc gtgaggtcgc gaaagcagag aagggcacca tgccgccatc tttatttcgg
     1081 acataccaga gattgatgtc atctggcagc atgcgatacg gcgcagctgc ctgcacatag
     1141 actttcttca cgcgaccgcg atccataaag tcattcacat agctcgaacc ccaggcggtt
     1201 tgcagtgtgt cgttaatatc gtcgatagca acgcccagcg cctgagcttt acgctggtcg
     1261 atatcaatct gcaactgcgg actgtcgtcg aggccgttat ggcgcacacg ggttagctcc
     1321 gggttttccg ccgccagcgc cagcaactgg ttacgtgctg ccatcagcgc atcgtgacct
     1381 gctccagcgt ggtcctgcaa ctccatatca aaacccgcag aactaccaag accgctaatt
     1441 gctggcgggc tgctggcgat aacgcgagct tctttaatct tgttaaacgc ctttgttgca
     1501 cgctcgataa tggcaaacga ggtgccggtc ttactgtcgc gttcgctcca gtctttcagg
     1561 cggataaaca ttcgcgccac gttttgcccg ttacccccag ggccagaacc aacggtggca
     1621 aacaccgaca tgatgttgtc tttttcatgg gtgaagtaat atttctcgat ttgctcaacg
     1681 actttcaggg tctgttgttg ggttgaaccg ctgggcaatt gtaccgaggt agtaaacatg
     1741 ccacggtctt ccagcggtaa gaacgacgtc ggcaaacgca ggaacaggaa caccatgccg
     1801 ccaagcagca ggacataaat cacaatccag cgcaggctgc ggtggagaat tttcgccacc
     1861 cctttttcgt agcgttcggc gttgcggtta aacatctggt taaaccaggc aaaaaagcct
     1921 ttttgcccgt gatgctcacc ttttttcagt ggcttaagta gtgtggcaca cagagccgga
     1981 gtgaggatca tcgctaccag tactgacagc accatcgccg caacaatggt aatagagaac
     2041 tggcgataga tggcaccggt ggtgccgccg aagaaggcca ttggtacaaa taccgccgaa
     2101 agcaccatcg caatcccgac cagtgccccc tggatctgcc ccatcgattt acgtgtggct
     2161 tcgcgaggag tgagtccttc ctcactcata atacgttcga cgttttccac caccacgatt
     2221 gcgtcatcca ccagcagacc gatcgccagt accatcgcga acatggttaa ggtgttgacg
     2281 ctgtaaccga aggcgtaaag tacggagaag gttcccatca acaccaccgg cacggcgata
     2341 gtgggtatca gcgtggcgcg gaagttttgc aggaacagat acataacgag gaaaaccagg
     2401 gcgatagctt ccagcagcgt tttcaccacg tcttcaatgg aggctttaac aaacgaggtg
     2461 gtttcatacg ccaccttgta ttccagtccg tgcgggaagt actgcgccag ctcgtcgaga
     2521 cgattgagca ccagctccgc tgtcgccatt tcgtttgcgc cggaggccag ttttaccccc
     2581 agcccggagg ctggcttacc attgaagcgg ctaagataat cgtatttctc cgcccccatt
     2641 tcgacggtgg cgacatcgcc cagccttacc tctgagccgt cttgattcac ccgcaaggtg
     2701 atatcgcgga actgttctgg tgtttgcagc agtgactggg cgttaatggt ggcgttgagc
     2761 gcctgcttat cgacggaagg tgtaccacca agttgcccaa ccgcaatctg cgcgttctgt
     2821 gactcaatgg catcagtgac atctttagcc gtcatctgga aactgttgag tttcgccggg
     2881 tccagccaga tacgcatgga atattgcgaa ccataggcat cgatatcccc gacgccattc
     2941 acgcggctta acgggtcctg aatattactg gcaacataat cggcaatatc ctgtttatcc
     3001 atcgaaccat cggtagagac gaaagcaatg gtcagaatat tggtatcgcc ggttttacgc
     3061 accgtcacgc cctgattttg caccgcctgc ggtaactttc gcatggctga ttgcagctgg
     3121 ttttgtactt gctgcacggc ttcgtccgga tcggtgcctg ctttaaaact taaagtgaca
     3181 gatgcctgac cgatgccact gctctgagat gacatataca tgagattatc gaggccggtc
     3241 atattttgct cgataacctg ggtcacggtg ttttccagcg tctgggccga tgcgccggga
     3301 tagttagcgg tcactcgcac attcggtggc gcgagatcgg ggtattgttc aacgggcaat
     3361 gaaaaaatcg ccagggtccc tgtcagacac aacaggattg ccagcaccca ggcaaaaatg
     3421 gggcgatcaa taaagaaatt cgccattaaa agaggacctc gtgtttctgc gtatcgttgt
     3481 aggcttgact tgcttcactg tagcggcaag gtacgagcca atcgtggaga aaaaaaggag
     3541 ttaatgtaaa ttatcatgcc cttttatact gaaccttaag tgcaagtatt ctttggtcag
     3601 taggaggcat tacatcaact gactttcctc accctccgca gagcgaaagc taatactcac
     3661 taacgtgccg ccgccggaag gtggtgaaaa agtcagcgtc ccacccagcc gttccgcgcg
     3721 ttcgcgcatg atattcagac cataatgacc ttcgggttct ttcggttcac cgataccaat
     3781 cccgttatca cggatataaa ccgtgtgatt gccgtccggc gcggtgacgc aactgacggc
     3841 gatttcgctg gcgttggcgt gcttcatcgc attcagcacc gcttcgcgaa taatttgcaa
     3901 caaatgcacc tgcatttgcg catccagcgc cagggttggc agacggcagt cgagggtcag
     3961 tttggcgctg gtttgatttt gtaacgtatc cagcatttcc ctcaacgcgg aggggagatc
     4021 cgcctgctgc agcgtcaggc ggaaagtagt caacagctcg cgtaactgcc gataagcatc
     4081 attcaacgcc tgggaaaaat cggccatgat actttgtgcg gtggcgttat cttccggtat
     4141 cgaacgcttc agtaacgtca actggatacg taagtaagaa agtacctgag ccagcgagtc
     4201 gtgcaattcg cgggcgatgg ttgcacgttc ttccatcaac aataactgct gaaaatgctt
     4261 ttgcgcctga ttaaagtaca aaccgcgtcc cagcatcgac gaaacgctgt taagcagcgg
     4321 ttctgatgat gaaacgtgac tattttgcca gtgcagttcg ccgtaaaccg tctcttgcat
     4381 tgtcaccggt aaaatctgca tcggcaattc cgggtttggt tgcccttcgc taatccgcca
     4441 gttttcaccg acatttaact ccagatattc agccgcttca ttgtcgcgaa caatctgcaa
     4501 aatatggcgg aaacaatgca catcaatctg gcttgtgttc agcgcctgcg aacattgata
     4561 caacacctcc agacgacgct tggcctcgtg gagatcgcgg gtcttttctt ctactgacgc
     4621 ctccagcgaa aggtacaatt tatgcagctc gctcgacatc tggttaaagg tttttgcaag
     4681 cagaccaagc tcattcggca gactggtatc cagcggcggc gagtcgaact gcccgtgttc
     4741 aatacgttga ctggcggtaa ccagctgatt cagcggggca accacctgat ggcgtatacg
     4801 gcgcaaagta aaaaagacca gcgtgaaaat accgataccg ccagccaggg aaatcgccac
     4861 caccagcagc attttgcgtt cagcgtagtg ctgtaaagcc agcacgaaca ggtctatctg
     4921 attaacataa ttattaatat tggcctgata ccacggcaaa tcgcccttgc tgagccgatt
     4981 attcatctcc agccagttgg cattcagatg cgcatagcga gtttttactg cttctggcac
     5041 ataccagacg ttgagattgg ttaataccga tgaatgcagt gcctgctgaa acaactggcg
     5101 atgtgcattg agttgtggac tgccactttg caagtcgtag cccaggcggt aactctgcat
     5161 acgcagcgat ccggcaatat tgatagcctc agcgtcgcgc aaactgctcg ccagagttag
     5221 cagagcgata cccgtggaaa gaatcgacag cagcacaatg taaaaaaagg cccgggccag
     5281 actggccgag acgggtcgtt taacaatcac acgcgtcttc tccacaaaaa atcattaatg
     5341 ttccagttcg ctaaaacgca atttagtcag tgatgggatt atttgatctg ccacagttta
     5401 tagcgaaata gttacttatt ctgggcacat gaacattgcc gattcccctg taagtcgggt
     5461 aataacaaca ctcatataaa gaataaggtt tttacaacca aaaaagaagg tcgttatgaa
     5521 tcgttttatt atggccaaca gtcagcaatg tctgggttgt catgcttgtg aaatcgcctg
     5581 tgtcatggct cacaatgatg agcaacatgt cctgagccaa caccattttc atccccgaat
     5641 tacggttatc aaacatcagc agcaacgtag tgcagtgacc tgtcaccatt gtgaagatgc
     5701 gccctgcgcc cgtagctgcc ctaatggcgc aatcagccac gttgatgaca gcattcaggt
     5761 caatcagcaa aagtgtattg gctgtaaatc ctgcgtggtg gcctgtccgt ttggtacgat
     5821 gcaaatcgtc ctgacacccg tcgcggcagg aaaagtaaaa gccacggcgc ataaatgcga
     5881 cctttgtgca gggcgcgaaa acggtcctgc ctgtgtcgag aattgcccgg cggacgcgct
     5941 gcaactggtc actgacgtcg cactctccgg catggcgaaa tcccgccgct tgcgcaccgc
     6001 acgtcaggag catcaaccgt ggcatgccag taccgctgca caagaaatgc cggtaatgag
     6061 taaagtcgaa caaatgcagg caacgcccgc gcgtggcgag ccggataaac tggcgattga
     6121 agcgcgcaaa accggttttg atgaaattta tctgccattc cgtgccgatc aggcacaacg
     6181 ggaagcctcg cgctgcctta aatgcggcga gcacagcgtt tgtgaatgga cctgcccgct
     6241 gcataaccat attccgcagt ggattgaact ggtgaaagcc ggaaacatcg acgccgccgt
     6301 cgagctttct cacctgacca acaccctgcc ggaaattacc ggacgcgttt gtccgcagga
     6361 ccgtttgtgt gaaggtgcct gtactattcg cgatgagcac ggcgcggtaa ctatcggcaa
     6421 cattgaacgc tacatttcag atcaggcgtt ggcgaaaggt tggcgtcctg acttaagcca
     6481 tgtcaccaaa gtggacaagc gggtggcgat tatcggtgcg ggtccggcag ggctggcctg
     6541 tgcggatgtt ctgacccgca atggcgtggc ggtgacggtg tacgatcgcc atccagaaat
     6601 tggtggcttg ctcactttcg gcattccttc tttcaaactg gataaatccc tgctggcacg
     6661 ccgtcgggaa atcttcagcg cgatggggat tcacttcgaa ctcaattgtg aagtgggtaa
     6721 agatgtctct ttggattcgc ttttggaaca atacgacgcg gtcttcgttg gcgtaggcac
     6781 ttaccgttcc atgaaagcgg gtttacccaa tgaagatgcg ccgggcgttt atgacgcgct
     6841 gccgttcctc atcgccaaca ctaaacaggt gatgggactc gaagagctac cggaagagcc
     6901 gtttatcaat accgccggac tcaacgtcgt ggtactgggc ggcggcgaca ccgcgatgga
     6961 ctgtgtgcgt accgcgctgc gccacggcgc gagtaacgtc acctgcgctt atcgtcgtga
     7021 tgaagctaac atgccaggct cgaagaaaga ggtgaagaac gcccgcgaag agggggccaa
     7081 cttcgaattt aacgtccagc cggtggcgct tgagctgaat gagcaaggtc acgtttgcgg
     7141 aattcgtttc ctgcgcacgc gtcttggcga gccggatgcc caggggcgcc ggcgacttgt
     7201 accgattgag ggcagtgaat ttgtcatgcc agccgatgcg gtgattatgg tgtttggctt
     7261 caatccacac gggatgccgt ggctggagtc gcacggtgta acggtagaca aatggggccg
     7321 catcatcgcg gatgtggaaa gccagtaccg ttaccagacc accaatccga aaatcttcgc
     7381 cggtggcgac gccgtgcgtg gtgcggatct ggtggttacc gcaatggcag aaggacgtca
     7441 tgcggcacag gggattattg actggctggg ggtaaaatca gtcaaatctc actgatagcc
     7501 tgcgcagaca aacccgactt cacagcgtaa tataattgtt catttcgcgc tgtggagtcg
     7561 gtatgacgca acaaatcacc ctcattaaag acaaaattct ctccgataac tatttcaccc
     7621 tgcacaacat tacttacgat ctcacgcgca aagatggcga agttatccgc cataaacgtg
     7681 aagtttacga tcgcggtaat ggtgcgacga tcctcctgta caacgcgaag aaaaagaccg
     7741 tggttctgat tcgtcagttc cgtgtcgcta cctgggttaa tggcaatgaa agcgggcagc
     7801 tgattgaaac ctgcgccggg ctgctggata acgacgaacc ggaagtgtgc attcgtaaag
     7861 aagcgattga agagacgggc tatgaagtgg gcgaagtgcg caaattattt gaactgtata
     7921 tgtcgcctgg cggtgtgact gagctaatcc acttttttat cgccgaatac agtgacaatc
     7981 agcgcgctaa cgccggtggc ggtgttgaag atgaagatat tgaagtgctt gagctgccgt
     8041 tcagccaggc gctggagatg atcaaaaccg gcgagatacg tgacggtaag acggtgttat
     8101 tgctta
//
LOCUS       DAZOYL010000181         8103 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZOYL010000181 DAZOYL010000000
VERSION     DAZOYL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.182"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            44..568
                     /gene="radC"
                     /locus_tag="ACHUOY_002859"
     CDS             44..568
                     /gene="radC"
                     /locus_tag="ACHUOY_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR0683588.1"
                     /translation="MNKLESLPGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYN
                     VRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEV
                     HPREIIREAIKINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVI
                     GRGEYVSFAERGWI"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="ACHUOY_002860"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="ACHUOY_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR0683589.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHUOY_002861"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHUOY_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR0683590.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="ACHUOY_002862"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="ACHUOY_002862"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR0683591.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHUOY_002863"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHUOY_002863"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR0683592.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHUOY_002864"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHUOY_002864"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR0683593.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4332..5390
                     /gene="rfaQ"
                     /locus_tag="ACHUOY_002865"
     CDS             4332..5390
                     /gene="rfaQ"
                     /locus_tag="ACHUOY_002865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR0683594.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5387..6511
                     /locus_tag="ACHUOY_002866"
     CDS             5387..6511
                     /locus_tag="ACHUOY_002866"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR0683595.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6504..7301
                     /gene="rfaP"
                     /locus_tag="ACHUOY_002867"
     CDS             6504..7301
                     /gene="rfaP"
                     /locus_tag="ACHUOY_002867"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR0683596.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7317..>8103
                     /locus_tag="ACHUOY_002868"
     CDS             7317..>8103
                     /locus_tag="ACHUOY_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0683597.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETV"
BASE COUNT         2081 a         1861 c         2053 g         2108 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
     4141 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc
     4201 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
     4261 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
     4321 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
     4381 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
     4441 aaatcgatgt gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta
     4501 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
     4561 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
     4621 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
     4681 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg
     4741 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
     4801 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
     4861 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
     4921 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
     4981 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
     5041 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
     5101 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
     5161 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
     5221 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
     5281 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
     5341 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
     5401 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
     5461 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
     5521 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
     5581 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
     5641 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
     5701 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
     5761 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
     5821 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
     5881 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
     5941 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
     6001 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
     6061 gcgccacaac acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct
     6121 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
     6181 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
     6241 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
     6301 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
     6361 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
     6421 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
     6481 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
     6541 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
     6601 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
     6661 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
     6721 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
     6781 atggggtggc atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta
     6841 ccgaagatct gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc
     6901 caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
     6961 tgcatgctgt gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
     7021 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
     7081 ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt
     7141 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg
     7201 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
     7261 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga
     7321 gtgcccacta ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc
     7381 cagcggcgtc agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt
     7441 ttggttgtgg tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt
     7501 tccacgtttt tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga
     7561 aaagctatcg tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac
     7621 cgacgaccaa aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta
     7681 ttgatcaaca agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa
     7741 agccgctgat aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg
     7801 ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact
     7861 ttaattcagg tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta
     7921 aagcgatgtc gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag
     7981 atatacttaa tcttatcctg tcagggaaag ttaaattcat tgatgctaaa tacaatactc
     8041 aatttagttt aaattatgaa ttaaaaaaat catttgtttg tccaattaat gatgaaaccg
     8101 tat
//
LOCUS       DAZOYL010000182         8018 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZOYL010000182 DAZOYL010000000
VERSION     DAZOYL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.183"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(69..1079)
                     /gene="pyrD"
                     /locus_tag="ACHUOY_002869"
     CDS             complement(69..1079)
                     /gene="pyrD"
                     /locus_tag="ACHUOY_002869"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR0683598.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(1190..1927)
                     /locus_tag="ACHUOY_002870"
     CDS             complement(1190..1927)
                     /locus_tag="ACHUOY_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0683599.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1893..2408)
                     /locus_tag="ACHUOY_002871"
     CDS             complement(1893..2408)
                     /locus_tag="ACHUOY_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0683600.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2416..2958)
                     /locus_tag="ACHUOY_002872"
     CDS             complement(2416..2958)
                     /locus_tag="ACHUOY_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0683601.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2970..4040)
                     /locus_tag="ACHUOY_002873"
     CDS             complement(2970..4040)
                     /locus_tag="ACHUOY_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0683602.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(4031..6630)
                     /locus_tag="ACHUOY_002874"
                     /pseudo
     CDS             complement(4031..6630)
                     /locus_tag="ACHUOY_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(6655..7356)
                     /locus_tag="ACHUOY_002875"
     CDS             complement(6655..7356)
                     /locus_tag="ACHUOY_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0683603.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(7439..7981)
                     /locus_tag="ACHUOY_002876"
     CDS             complement(7439..7981)
                     /locus_tag="ACHUOY_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0683604.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT         2067 a         2031 c         1771 g         2149 t
ORIGIN      
        1 gaatataaaa caaccagggc tgaaaataaa gccctgatta tcttataaag aggaattcga
       61 agaaataatt agatatgggt aacgatttct ttaatcagcg gcggaccttt aaaaataaaa
      121 ccagaataaa tttgcaccag tgaggcaccc gcagcaatct tttcacgcgc agcgataacc
      181 gagtcgatgc cgccaacacc gatgatcggt aagcgaccgt ttaattccag tgacaagcgg
      241 cgaataattt cggtgctttt taactgaagc ggacgaccac ttaagccacc ggtttgatcg
      301 caatttttca ttccctgaac aagagaacga tcgagtgtgg tattggttgc aataacgcca
      361 tcaatattat ggcgaactaa actatcggca acctggatca attcttcttc agaaagatcc
      421 ggcgcgatct tcactgcgat cggcacatat ttatggtgca tcgcttgcaa atcattttgc
      481 ttatttttaa tcgcggttaa gagatcatcc agcgcttcac catattgcag cgtgcgtaat
      541 cctggggtat tcggcgatga aatattgatg gcgatatatc ccgcataggc atagattttt
      601 tccatacaaa tcagatagtc atctttgccc tgctccactg gcgtatcttt atttttgccg
      661 atgttaatac ccaggacgcc gtcataatgg gcctttttta cgttctctac gaggttatca
      721 acgccaagat tattaaagcc catacggttg atcaaacctt cggcatctac cagacgaaag
      781 agacgcggct tgtcattacc tggctgtgga cgtggcgtga cggtaccgat ctcgatcgat
      841 ccaaatccca tcgcgcctaa cgcgtcaatg cactccccgt ctttatcaag accggctgcc
      901 agaccaagcg gatttttaaa cgtcaggccc atgcagttaa caggtttcgc aggcactttc
      961 tgccgcacca gtgcttcaaa cggcgttcct gtaatacggc gtaattgctg aaaagtaaac
     1021 tcatgagcgc gctctggatc gagctggaaa agggctttac gaacgaaggg gtagtacatg
     1081 aactctcctg gattcccggt gtgcaaaccg ggggcgtatt atgtgcgagt tcggaccaaa
     1141 agggaattga cctgcggcaa aaaaaacgca aacgttttcc gaacctgttt cagggcagat
     1201 tttgctcctt ctttgcactc aacccgccgt aatcatttat cgcccgccag gcgacatgac
     1261 acggtgcgat tgcctgacac cagcttgtct gacgctggct tttaggcggc acaactcccg
     1321 cattacttaa cgctttacca tttacgctca cctgaatcag gttaatgtag taaggcgttg
     1381 ggttagtgag ttgtacaccc tgcgaattcc gtgtccagcg taattgttga taagcttcca
     1441 gcgggtcgcc cggcaaacct tcgggtcgcc agaacagttt aaataccgag cgcatcgcca
     1501 cttttacgct ctgattttca actttgccgg atggcacgct ggcaatgctt aactcaaata
     1561 gcgtttcgcg atccacaggc aagatgtcgc tttccgttct cagcaagcgc aacgtgcctg
     1621 tagtaccggg cttcaggaga ataagtggcg gagcggccag taatggtgct ggcactgtcg
     1681 acgcttcccc cccggcccaa cgcgttgggc ggttgatttt actgttaata agccaggatt
     1741 cctgactggt attagtcagt aaaatagata tcgattctct gtctgccgga aagataaatc
     1801 gcgttccgcc gaccaccact cctgcccgca attcacccgc caccagcagc gataaaacgg
     1861 cgaaaatcaa gagcgccaat ctgttccacg tattagtcat aatgaagtac aaatgtcgcg
     1921 ctggcattca ctttacccga ctgcacatcg ctattgacag gtcgcaattg cgcataaaaa
     1981 gttaatggca ccttaccgct atgtaggctg tagtctttac tcgcctggtt aagcggaatc
     2041 aatatcttcg catcatccag aatcgcaatt gccagcccgc tggctgcatc cgtaccactt
     2101 tcaatggaca gcatttcgct ctgcactccg tcgggggttc ctgaaaaggt gacgtcaacc
     2161 gtgctgacat ctgttccgca atcctgcaaa ttaatgatga aagatttctc cgggccataa
     2221 ttcccatgaa aaaaacgtgt cagcgagaca tcgcctaact gcacattgat ttgcttgcta
     2281 tcctgtgcca gcacgcatgt attacggcga aacgatccgc taatgttgac cgtgccatca
     2341 tgggcaaata gctcacagcc ccccgttaat cccagtaaga acagaatcca gattatccgt
     2401 ttcaacatca ctccttcact ggtaaaccag atcaaaatag agaaccgccg tcgcattacc
     2461 gcccgttgct cccgcctttg tggacttata acgaagttga tatttgagtg tgttatcccc
     2521 ggcttttaag ggcgtaagag gctggacctg attgagcggg atttcttgct gactttgcgc
     2581 atcaagaatt tgcacggcaa tccccgttgc cataccaccg cttccgcctg tgtcagttag
     2641 tttgagcagt gtcggcgtaa gggtgtcagc ctcgccgcta aaaaggacgt ccgcgccagt
     2701 aatactagcg gcacaaccgg tgatatcgat attcaaatca gcagcagtgc ttacggtatt
     2761 cgcggcctga aaatcactga tattaaaatc accaatatga atattttgta atgcactttt
     2821 ggtggcgaca tcacagccct gatcgaccac attcccataa atatgcatac taacttgttc
     2881 aggcccagcc atcccgacat ggatgttaag aatgctaaag aacaagataa cgcactgaaa
     2941 tatcgtttct ttcttcatac gttcacgcgt taatcaaaat ttattctcac cgttgccgtg
     3001 gcttcaaatc gcccggtttc tggtggtaca cctgtcgtgc tgactggata gacattcatt
     3061 cgcgcgatac tgtctgcatc atcaggaata gagagcggta atgttccacc agcaggggaa
     3121 ataatgttgt tctggctatc ggtcaccacc acgcccacgc caggacgtga cgtcttaatc
     3181 gcctggggat agctggggtc tgtggttgcc gtcagactca ggttgacgtt aactaacccc
     3241 gcgtttcccg tgttacacct gactggaatt gcaagttctg ccgttctggc cccaagaggc
     3301 ttatgcccta caacacgaaa gttagccgca aatgcgtcgc ccagatcgac ctcgattgtt
     3361 tctccggcat tgacggaaca tgagccaagt gaattgatcg taccagacag gtaatacact
     3421 aaaaccggtg tacctgtagt cgtgcaggaa tcacctgcgg gtatgttgta gcattcaaat
     3481 aatcgcgcca gcgccatagg aggaatgacg accgaaccaa cgaacggctg caatattttc
     3541 agggaaaagc taccgctatt accgctagcc ccctggccac ctaaacgttg accgccggga
     3601 tcgcaaatat gccatcgtgt atctgcccct gtgcccacgt tttcgaaagg aaccgcagta
     3661 ggagaactgc ctttaacctg caataaaact ttggcggcaa gatagtcatt aattttgtac
     3721 cagttgtcgc ctaaatagac taacggtgaa tccgcggcga atgcccatcg gccacttctg
     3781 acattgtctt tatcgcaatc acaggaaaca taataatcat tcgattcgtt ccactgataa
     3841 aatttgtctt taactactcc agccgtattt tcttcttcct ccagggtttt aacgccaaaa
     3901 ttgacgccaa tatacggatt cccctgagtg gtatgacact gcccgctata agcagcagtt
     3961 tgagcgcacc atagcattaa gacggcggca aaaaatagtc gtagtaataa cacgcatttt
     4021 tctccaaaga ttatctgcat atggcatcca ttcgcgtaat cgcagagtta atttgttgtt
     4081 cggtaagctg gtaactgata tgacaaattt gatcgggagc ttcgccccag cgtacagtta
     4141 tcgctccctg agcgggcaaa ccggacatat agagcgagcc atcatcatca ataatgccgc
     4201 tattagtctg tacgccgtcc agcgtcgcta tcgcgccaaa acgcagcggt ataccctgct
     4261 ttgatgtttt cattaatacc cttgctcccg catgggcatt gaactccgcc agaaccaacg
     4321 cccctttggt gggaaccacg ttgactactg cattttcaag atccacgtta cgttttaacg
     4381 atgccgcatc aagtgcgatt cgattttccc gataagaagt ggcatagggg acaacggtat
     4441 atccgcgcca gtcagttttc agcccggtgc tgttcgccac acgagtattc tctgcgcctg
     4501 gtgctgcaat cagcacgtta gtttccccca gcgattgccc cagggtaatg ccctgtgaat
     4561 gggcaactaa actgcctgaa agagcgtagt tcagttgttg ttaactgccg ttatcgctgt
     4621 agttgtagcc cactcgggca tctgcaaacg cccctttgta gtccatgctg gcgctaccat
     4681 tagccgtttt cccctcgctg ttatatccct gttgcacgct gtagctcagg ttaccgtcat
     4741 cgagtagtgt ctccgttaaa cccaggtttt gtgtaatgtg cccttcgtta tcgatgctgg
     4801 tgttagaggt catccaggcg taattttttt cctggtatag ctatcattac ttctggggag
     4861 caacagattg agcggcaggg aaaaatttag tgcaaacacc tgatcagcat caggttgacc
     4921 acgggactta ctgtagttcc aggaaccccc cagcgagaga tctttaattt gcgtgttgta
     4981 gccaaactgt aatagccgat cctgttgatc ggtatgccag taagtttgct ggctaccact
     5041 taaataaaac gaaccgtact cgcctaattg ctgggagata ttgacctgca gtttgccacg
     5101 tttggtgtaa aacaaattgt aataacgcga ccacatcggc gtatcttcat catcaccgtc
     5161 attaaattcg tagccatcca tatgtttata catggtgtcg gaaagggtgt agaaacccga
     5221 cgtcgaatag cgatagccca tcaattgaaa attagtgccc aattggttga gcgttttggc
     5281 atacagaaaa cgatatgact gacctttatg ttcgctttgg tctgccagcg tactcttcgc
     5341 ctgggtcgca tcgaacgaaa ttgctccgaa atcgccaagg ttaaatccca gaccaaacat
     5401 ggcggcacgg taatattcag catattgtcc accaccatac catgtcgttc cccacggtcc
     5461 gccccactgc aacgtggcct aggcaaattt gctctcctgc tgttcattac tattggttct
     5521 gtatttcgcc agtgtcaccg cgtatttgat tcgcccctga cgctggagta atggcacgct
     5581 ggaaaacggt acgctgtagc tattgacgct gccgtccgct tctttgattt caactaacaa
     5641 atcaccgctc gacgacgtgg aatagagatc actaatttca aaagcaccag gcgatacata
     5701 agtttggtaa atggtgtaac cattttgctt aatcgttatc tgggcattgc ttttggcgat
     5761 accacgcacc acaggcgcaa accctttcag gctgtctggc agcatgttgt catctgacgc
     5821 cagctgcaca ccacgaaagc tgacactatc gaaaaaatcg cctgcagtat aatcatcacc
     5881 tacggtcagt tcgcctttta gtggaataac cgcccgctgc aaatagctgc tgagattctt
     5941 ccattctgcg gtttggccac tactgcgact ccatgttgaa ttgttgcgca atctccatgg
     6001 gcctaaatta acgccactgt ttaaattcag aaaatagctg tcgccagaat cactgtctgc
     6061 gctgctatga atactgttag ccccgctaaa cgaatatccc agtaatagcg cgttgatccc
     6121 ttcatcccaa cgttcagggg ggacataacc acgggcatta cttttgatgg cgatttgcgg
     6181 tactgaaatt tccagacgga gtttattcac attaaacgtt acgctggcat ccggaataat
     6241 ctccgccaat ggcacacacg cttgtttgtc cagttgttta aaagccggaa acgcatcagt
     6301 attaacaccc attgcatcca ggctttcagt cgtcaaacag gcggcaatcc ccttctctgg
     6361 cgttatctca acaaaattga cgttgcgcgt atctacaatt gtctgattca gaacaatatc
     6421 gacacgataa acccctggcg ttattttttg tcctttttcg aaacgagaca aatcggccac
     6481 cattgaggga tcatctttga aaaaagcagg atcgaaccag gtttcagctt gtgcattgaa
     6541 cgctgccgac acaaacacca ccagcccacc aataaacgat ggcactccac cagagcttaa
     6601 caggctgtgt tgtcgatgag ttctatacat ctcgctctct taactgaaga cggcttacga
     6661 tttacagttg agttgtggcg tttccgcccc gtaatcattg atggttcgat acgttacgct
     6721 gctatttttg gccccctttg gcagagagaa ttggtattta tcaaaggggg aaatcatttg
     6781 atttttaact acctttcccc ctattttcag gtctgtcagg gtaatgaaat aaggtgttgg
     6841 gttactgacc gtaaccatgt cagctgacga agtgcaacgc agtttcgccg gagcctcgcc
     6901 taagggatac gctaaacctt ttggtcgcca gaaaagtttg atgacgcttt gtgtggcaat
     6961 cttcagggta tttccctctt cccgcttcgt tgttgaaggg actgcccgta cgctagtgaa
     7021 gaaaagggtt tctctgtctt tcgccaacgg cgcaccaatg tacataatgc gtaacagatt
     7081 ctcattagcc ggattcagca caaatagcgg tggtgtaata ataaaatctg ctgatttttt
     7141 attatctggg gtcatcaccc atgactgaat aagataagga acatccggac tggtgttacg
     7201 tacacccagt gaaatttgct cttcaccttc tgaataaaca agacgtgttg ctgcaagtcc
     7261 aatcccgcct ttacctgccg cccatgcgga tgagcagctg agtaatattg ccgttaaact
     7321 tacggtcaca attccctttg ttatgcaagt tttcataaat agtcactctt aaaaaatatg
     7381 aatatcccct ccagaacact agaggggtac taattaataa aacaattaat gagttctatt
     7441 aataatagtt aatatggaaa tttgttaccg cattcacatt accagaagtc gctttccccg
     7501 tagcgatata atcaacttta aaggtcactg tttgctcagc agaatccgct aactgaatat
     7561 tcgacgtttc ggtaccggga gttaccgatg tagaaccatc tggcagatac atttgtagag
     7621 caacattcgt cgctgcatcg gtggtagcct ggttggcaaa tgctgtaggt tgcacgccgt
     7681 cagccgtacc actaaaggta aacgtcgcat ttttagttac agtaacatca cagccttcta
     7741 aggcaattgg tacatcagtt ttaacgccag agactgctcc attagtttgc aacttggtgg
     7801 cgctgacatt tggcaatgtg actactgaat ctttcgtcgc agctaccagg gtacaagctg
     7861 gagcaataac tttaccatta aatgttactt tgccatccgt gatagcatta tcatcagctg
     7921 ccataacgct ggacgttgca cataccacag ttataaacgc cgtcaataca ctttttttca
     7981 tcgtcatcat cctgatctct tcgtaaatat gataatgc
//
LOCUS       DAZOYL010000183         7986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZOYL010000183 DAZOYL010000000
VERSION     DAZOYL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.184"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1340
                     /gene="yjeM"
                     /locus_tag="ACHUOY_002877"
     CDS             <1..1340
                     /gene="yjeM"
                     /locus_tag="ACHUOY_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0683605.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(1491..4814)
                     /gene="mscM"
                     /locus_tag="ACHUOY_002878"
     CDS             complement(1491..4814)
                     /gene="mscM"
                     /locus_tag="ACHUOY_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR0683606.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4836..5804)
                     /gene="asd"
                     /locus_tag="ACHUOY_002879"
     CDS             complement(4836..5804)
                     /gene="asd"
                     /locus_tag="ACHUOY_002879"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR0683607.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5901..6953)
                     /gene="rsgA"
                     /locus_tag="ACHUOY_002880"
     CDS             complement(5901..6953)
                     /gene="rsgA"
                     /locus_tag="ACHUOY_002880"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR0683608.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7048..7593
                     /gene="orn"
                     /locus_tag="ACHUOY_002881"
     CDS             7048..7593
                     /gene="orn"
                     /locus_tag="ACHUOY_002881"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR0683609.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7804..7879
                     /locus_tag="ACHUOY_002882"
     tRNA            7804..7879
                     /locus_tag="ACHUOY_002882"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7837..7839,aa:Gly,seq:gcc)
     gene            7915..7986
                     /locus_tag="ACHUOY_002883"
     tRNA            7915..7986
                     /locus_tag="ACHUOY_002883"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7948..7950,aa:Gly,seq:gcc)
BASE COUNT         1821 a         2077 c         2153 g         1935 t
ORIGIN      
        1 taatgatggc tgaaatggga gctgcttatc gcaaagaaga aggtggtatc tattcctgga
       61 tgaataatag tgtcggacca cgttttgcct tcattggtac gtttatgtgg ttttcctctt
      121 atatcatctg gatggtgagt acctccgcga aagtttgggt accgttctca acattcctct
      181 atggtagcga catgacccag cactggcgta ttgccggact ggagcctacg caggtggttg
      241 gtctgctggc agtagcatgg atgattctgg tcaccgtcgt tgcttcaaag gggattaata
      301 aaattgcccg cattactgcg gtgggcggta ttgcagtaat gtgtctgaat ttagtattgc
      361 tgttagtaag cattactatt ttgttattaa atggtgggca tttcgcgcag gatattaatt
      421 tccttgcatc accgaacccg ggttatcagt ccggtctggc aatgctatcg tttgtggtat
      481 ttgctatttt tgcctatggc ggaattgaag cggttggtgg tctggtcgat aaaacggaaa
      541 atccagaaaa gaactttgcc aaaggtattg tttttgccgc tattgttatt tcaatcggtt
      601 attcgctggc aatattttta tggggcgtca gcacaaactg gcagcaggta ttaagtaatg
      661 gttccgttaa ccttggcaat attacctatg tgctgatgaa gagcctcggg gtgacgctgg
      721 gtaacgcact gcatttgtca cctgaagcgt cattgtcgct gggcgtatgg tttgcgcgta
      781 ttaccggact ttcgatgttc ctcgcttata ccggtgcgtt ctttacgctt tgctattcac
      841 cgctgaaagc catcatccag gggacgccga aagcgttgtg gccggaaccg atgacgcgcc
      901 tgaatgcgat ggggatgccg tctatcgcca tgtggatgca gtgcgggttg gttactgtct
      961 tcatcctgct ggtttcgttt ggtggcggta ccgcatccgc gttctttaac aagctgacgc
     1021 tgatggcgaa cgtgtctatg acgcttcctt acctgttcct cgcgctggct ttcccattct
     1081 ttaaagcacg tcaggatctc gacagaccat ttgtgatttt caaaacgcgt atgtcggcaa
     1141 tgattgcgat ggtggttgtc gtactggtgg tgacatttgc gaacgtcttc accattattc
     1201 agcctgtggt tgaagccgga gactgggaca gcacattgtg gatgattggc ggccctgtct
     1261 tcttctcgct gttagcgatg gcgatttacc agaactattg cagtcgcatg gcgaataaac
     1321 ctgagttagc tctcgactga tcatatgaaa tcctgcaact catcgtctct ccggcgatga
     1381 gttgtcagga agtaatgcgt atggatgata ctctccaggg ccggtgataa ctgaagatga
     1441 tgttaagctg ctaaatgagc cacgttgaca tcatttggca gcttcatgca ttacaaactt
     1501 cccgcctgac gacctttgcc cgcagacgtc agcgttctcc ccgtttgttt accgttgagg
     1561 ctttccagac gcatctggaa gggcgggaat ggcatatcga taccgtgggc atggaagcca
     1621 gccagaatca gctggtggat ctcatggcgt agcggcatac ggtgacccat ctcagcggca
     1681 taaatacgca gctcgaaaat ctgaatccct tgttgcagat ccaccaggaa gacttccggt
     1741 gccgggttgt cgatcaccag cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact
     1801 tcttcactgt tggcatcagc aggggccgga atcgtcaaca ctacgcgcgt gaccgagtca
     1861 gagagcgacc agttgataaa ctgctcggta ataaacgcct tgttcggcac gattatctct
     1921 ttacggtccc agtcgctgat ggtggtggcg cgggtgttaa ttttcgtcac gctaccggtg
     1981 agatcgcgaa ttgtcaccgt atcgccaatg cgaatcggtt tttcgaacag gataatcaga
     2041 ccagagataa agttggcgaa aatttcctgc aaaccaaaac cgagaccaac accgagcgcg
     2101 gcgaccagcc actgcaattt cgaccactca ataccaatca tcgagaagcc gaccagcccg
     2161 ccaatcagca tcagcagata tttggtgatg gtggtgatgg cgtagcccgt acccggcgtt
     2221 aaatccaggt gctgcaaaat cgccagttcc agcagcgcgg gcaggttgcg caccagctgc
     2281 gtggtgatga taaacaccag aatggcaatc agcaccgcac cgagggtaat tggctccaga
     2341 ctttctacgc cctgtaccgt ggaggtgaca tcccacagcg aaatattttc gaggaagccg
     2401 aaagcggaat ggatttctga ccacagcaca atgacagaaa gcagggcgat caacatcaaa
     2461 attgagcgca ccagccgcaa ggattgcgca ctgatggcat cgagatcgac ttcgctttca
     2521 tcgacttcaa ttgctccttc cgggctactg tgatgatgcg cttcctcttc gccacgcgca
     2581 cgttgcgcta acatctctgc ccggcgatgc ttcgcccggt caaacgccag cctgcggcgc
     2641 tggatcagca tccagcggcg gataacgtga taaaccacca gtagcaggaa ccagatggca
     2701 accgaggttt caagcctcgc taacagcgcc tgtgccgttg ccagataacc cacagccgac
     2761 gccagaatgg caaccaacgg cgcgccaatc atcatgttcc acagcatatg gttggtaatg
     2821 ttgtcgccgc tgccttcttt gttgagatac agcggaatcc cggccttttt caggctgagg
     2881 gtgaccaccg ccagcgcacc gcaaatgagg ataaagcaaa gccttcccag cgaaccggag
     2941 aattcacggt cgtcgaggtt atcgaacatc atcagcgcca taatcagcgg cacaataagc
     3001 ccgatgctca tcaggtagta gcgcatccca cgagatacgc gttcgcgcgg ccagccaaaa
     3061 tgagcgataa acaagccgtt cgggcgggca aaggtggcgc aaatcatcac tacccacagc
     3121 agcggcacgg tggccgttac accatcgcca atcgcgaccg ccagcggata aggccacgcc
     3181 tcgcgcaagc cgtaacccag cgtcatccac agcaccggta acggtgacgc gacgagaatc
     3241 gaccagaaaa gagtgcgcaa cgtcagccag aagtgatcct gagtcacttt gccaactttc
     3301 gccgccgaac gttcaagaaa acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc
     3361 agaatcaacg cgccaaacag cggcaaaatt gtctctttgc tggtcagcat catcacactg
     3421 gctttgccca actgactgaa ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt
     3481 tccagcggcc aggcgatggt catcggacgc acgtcagagg tccagaacag atagcggtgc
     3541 gttgcttcgt tcacctcttt cagcgcatcc tccagttgcc cgttggagac tttcagcttg
     3601 gtcagttcca atagtagcgt gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga
     3661 gtgcgcagtt gtgcttccag aatacggttt tgctcggcgg tcagcggctg accgtcggcc
     3721 tgatggattt gccgtagcag cggctgttta ttaagcagat cctcataacg taaccgttgc
     3781 acacgcaact gcgccatttc ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt
     3841 gccacctgcg ctcgcaacgc ttcaccgagc agattggacg atcccagcca ttgcgactgt
     3901 tcacgcagcg tattcaacgc ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt
     3961 tgcgaggcaa cgagatccat ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg
     4021 ttaattttga attgcgcgac gatatctttc ggcaaatcgg cactgttttc tgccagtaat
     4081 tcggtacttt ccagcgcccg ctccgcctca agctgacgtt ggctattcaa ctgattacgt
     4141 aaggcctgca aatacgcatc cagttgctgg ctctcttttt ccgcaagctc tgagcgtaag
     4201 cgcgctaatt cttggcggtt attggcagac agctgcgcca gctccagttc atcaacgagc
     4261 gccttaagac gtgcagagtc agactgcaac gcgaaatttt gtgcctgatt gagcggagta
     4321 ttgccggtaa gcgttcccag gcggcgctcg atctcattta actgacggcg ggcgtcggtt
     4381 tgctgttgcg gcagttgatt cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc
     4441 tgggcctgac ggcttttatc cagcaactgg ctgctgacct ggagaatttc ctgattcagc
     4501 gcgtcggtag acattcccgg cgacacgctg cgcggcttgt cacgcatgtt gtttaattgt
     4561 gcgcgcagag tagcggagag tttcggataa ttatcgataa cttgctgata ttgtttgatg
     4621 cgctcaaggg aaccttttcg ttcctcaagc gcatttaagg cagactgaag cgcctctacg
     4681 acttccggct gtgcgggttt cgccgctttt gcctgctcca gttcctgagt gatttgtttg
     4741 ctatcggggg ccgtcgcggc gtacgccccc caactgaggc accaggccat cagaaaagtg
     4801 ataatcaggc gcacgtcagc gtttcctttg atggattaga cctggtcttt tttgtcgtca
     4861 accaatgggc tggcgtcgtg ttctgcttcg atctcttcag caggaagcgg ggcaggttca
     4921 gcgtctggcg taacaaaggt ttcggtagat actgccagcg gctggcaaat tttcgtgaca
     4981 gacaggcttt ccagttgctc aaccagattc actttacccg gtgcaaacag gttgataacg
     5041 gtggaaccga gtttaaagcg acccatttcc tggcctttca gcagtgccac agaaccgtcg
     5101 ttttccccgg caggccaggt ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg
     5161 cccgcccaga ccgtctcaat gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt
     5221 gggccaaatt cggtatcgaa aaggcaaatc acgcgttcgt tacgggcaaa cagattcggc
     5281 acgttctgag ccgtgagatg gttaacggag aagagatcgc ccggcacgta gatcatctca
     5341 cgcagaatac cgttgcacgg catgtgtacg cggtggtagt cacgcgggga gaggtaagtg
     5401 gtcacaaacg taccgttgcg gaacaggtcc gccatcagat agttgcctgc cagcagggct
     5461 tcgaggctgt agttgtggcc tttggcttgc aggattttat cttcttcgat tttacccaac
     5521 tggctgataa cgccatcggc aggcatgacc agtacattcg gatcggtatc gattgggcgt
     5581 acttcgtcac gcagcggacg gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc
     5641 ggcttttgcg cctctttcat gtcgaccttg tagtatttaa cgaacagatc gataaccagt
     5701 tttgtcaacc atcctgcccg cttgcttgcg ccccaacccg ccaggcgagt aagccatagt
     5761 ttcggcagaa tgtactgtag cgaaagttta aatgaattta acaaggtagc ctccaggcca
     5821 ttgttttgtc gttcctgatt cggcctacat gccggatcct gaaaaaaagg ggacgattct
     5881 aacgacggtt agcttaattg tcagtcatcc gtatcagaaa agtttttacg cgtttttacc
     5941 tgcgccatgc tttccagaat acggtgatag ttttcgaaac gggtttccgc gattttccct
     6001 tcctcaaccg cttcccggat agcgcagccc ggatcggtat cgtgtttgca atcgcgatat
     6061 ttacacagac ctaaatagtc atggaattcg acaaagccct gagtgatttg ttccggctcc
     6121 aggtgccaga ggccgaactc acgcacacca ggggaatcaa tcacatcacc gccgtgcggg
     6181 aagtgataca gccgagcggc ggtcgtggtg tgctgaccaa gccccgagtt gtcagagaca
     6241 tcgttggtca ggatctcttt ttgcagcccc agcaacgcgt tcagcaggct ggatttgccg
     6301 acgccagact gcccggcaaa aatgctgatg cgcccggtca acgcctcttc cagcggtttt
     6361 agcccatcct gagtatggct ggaaaccatc aatacgcgat aaccgatatt gcggtagata
     6421 tccatctgct cgttgacgaa cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc
     6481 acaataatcg gctcaatctg caaggtttcg caggccacta ggtaacggtc gataatattg
     6541 agcgacagct ccggcaaaat ggcggagaca atgacaatct ggtcgatgtt ggcggcaata
     6601 ggttttacgc cgtcgtagaa atccggacgc gtcaacaccg aggtacgctc atgcaccgct
     6661 tccacgatcc ctttgacatt tacgccttcc gccgccggtt tacccggacg ccagactacg
     6721 cggtcgccgg ttaccagcga acggatggta cggcgaatat tgcagcggtg aacgtcgcca
     6781 tcggcggatt ccacatcagc gtgcatacca aagcggctga tgacgatacc ttcatcaggc
     6841 tcgccaaaca gattgtcgtc gtagtcgggc ttctccttag acgttttaag acgacgctgg
     6901 tgattggcgt tcacgcggcg ctgctggcct ttggagagtt tatttttact caatcgtact
     6961 gactcctggt cgcccctgat gggcaaaaca tctatgatac acgcaattgt ggatcaatat
     7021 agtcactgtg aatgggtgga aaatagcatg agtgccaatg aaaacaacct gatttggatc
     7081 gatcttgaga tgaccggtct ggatcccgag cgcgatcgca ttattgagat tgccacgctg
     7141 gtgaccgatg ccaatctgaa tattctggca gaagggccga ccattgcagt acatcagtcc
     7201 gatgaacagc tggcgctgat ggatgactgg aacgtgcgca cccataccgc cagcgggctg
     7261 gtagagcgcg tgaaagcgag cacgatgggc gatcgggaag ctgaactggc aacgctcgaa
     7321 ttcttaaaac agtgggtgcc tgcgggaaaa tcgccgattt gcggtaacag catcggacag
     7381 gaccgtcgtt tcctgtttaa atacatgccg gagctggaag cctacttcca ctaccgttat
     7441 ctcgatgtca gcaccctgaa agagctggcg cgccgctgga agccggaaat tctggatggt
     7501 tttaccaagc aggggacgca tcaggcaatg gatgatatcc gtgaatcggt ggcggagctg
     7561 gcttactacc gcgagcattt tatcaagctg taaaattttt cctggtcacg taagcgccgg
     7621 aatccacttg cggcggcgtg attttgacgc taaattgaac gtcttgctga taatttcagc
     7681 acttgagata aaaacgcaaa aaaaactttt ttggggggtt gcagagggaa agatttctcg
     7741 tataatgcgc ctcccgtaac gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag
     7801 tacgcgggaa tagctcagtt ggtagagcac gaccttgcca aggtcggggt cgcgagttcg
     7861 agtctcgttt cccgctccaa aatttgaaag tgctgtaagg cacagaccac ccaagcggga
     7921 atagctcagt tggtagagca cgaccttgcc aaggtcgggg tcgcgagttc gagtctcgtt
     7981 tcccgc
//
LOCUS       DAZOYL010000184         7968 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZOYL010000184 DAZOYL010000000
VERSION     DAZOYL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.185"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHUOY_002884"
     CDS             <1..239
                     /locus_tag="ACHUOY_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR0683610.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="ACHUOY_002885"
     CDS             279..1142
                     /locus_tag="ACHUOY_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0683611.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHUOY_002886"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHUOY_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR0683612.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="ACHUOY_002887"
     CDS             2679..4037
                     /locus_tag="ACHUOY_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0683613.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="ACHUOY_002888"
     CDS             4180..5130
                     /locus_tag="ACHUOY_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR0683614.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="ACHUOY_002889"
     CDS             5140..6522
                     /locus_tag="ACHUOY_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0683615.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6738..6812
                     /locus_tag="ACHUOY_002890"
                     /pseudo
     CDS             6738..6812
                     /locus_tag="ACHUOY_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="ACHUOY_002891"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="ACHUOY_002891"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR0683616.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2019 c         2308 g         1722 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggcgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctg
//
LOCUS       DAZOYL010000185         7936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZOYL010000185 DAZOYL010000000
VERSION     DAZOYL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.186"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="ACHUOY_002892"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="ACHUOY_002892"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR0683617.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="ACHUOY_002893"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="ACHUOY_002893"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR0683618.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="ACHUOY_002894"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="ACHUOY_002894"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0683619.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="ACHUOY_002895"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="ACHUOY_002895"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR0683620.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3010..4599
                     /gene="prfC"
                     /locus_tag="ACHUOY_002896"
     CDS             3010..4599
                     /gene="prfC"
                     /locus_tag="ACHUOY_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR0683621.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4992..5597
                     /gene="osmY"
                     /locus_tag="ACHUOY_002897"
     CDS             4992..5597
                     /gene="osmY"
                     /locus_tag="ACHUOY_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR0683622.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5724..5885
                     /locus_tag="ACHUOY_002898"
     CDS             5724..5885
                     /locus_tag="ACHUOY_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR0683623.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6007..7080
                     /locus_tag="ACHUOY_002899"
     CDS             6007..7080
                     /locus_tag="ACHUOY_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR0683624.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7077..7859
                     /locus_tag="ACHUOY_002900"
     CDS             7077..7859
                     /locus_tag="ACHUOY_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0683625.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1895 a         2047 c         2152 g         1842 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt cacgccgcat
      241 ccgacacggt attcggcgag ataattaacc tttcttcgcc tggcgggtca taatggcgcg
      301 atacaccttg aaacgcccgg tttgcgcaat cacttcatgg aagccaaatg tctcatccag
      361 cacgtccggg taaggcagga aggcgttcgc tacaattcgc agctcgccgc cgctattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacacagtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acccccgcgc cacagccgac atccagcact ttacctttcg tgtgcggagt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat cggcatcaaa
      841 taccggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatctg tccccactgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc atatcgcaat ctgcgacgtc
     1081 atccaccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacag atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa acctttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgcg gtcgatcctg actatcagcg tcagggattg ggaagggtgc tgctggaaca
     2041 tctgatcgac gaactggaaa aacgcggcgt ggcgacacta tggctggaag tccgtgcctc
     2101 aaatgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gttttgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgccaaaatc
     2581 ggcatcatca ccaacggctt tagcgccttg cagcaagtgc gtctggaacg cacgggcctg
     2641 cgtgattact tcgatttgct ggtgatttcc gaagaagttg gcgttgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaacaggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacctgc
     2821 tggctgaatg cgcacaatcg cgagcaacca gaaggcatcg cgcccacctg gaccgtttct
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc acccggatgc cggtaagact accatcaccg agaaggtgct gctgttcgga
     3121 caggccattc agaccgccgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg
     3181 gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt
     3241 ccgtatcacg attgcctggt taacctgctc gacaccccgg ggcacgaaga cttctcggaa
     3301 gatacctatc gtaccctgac ggcggtggac tgctgcctga tggttatcga cgctgcaaaa
     3361 ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc
     3421 ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact gctcgatgaa
     3481 gttgagaacg agctgaaaat cggctgcgca ccgatcacct ggccgattgg ctgcggcaag
     3541 ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctctatca gagcggtaaa
     3601 ggccacacga ttcaggaagt ccgtattgtt aaagggctga ataacccgga tctcgacgct
     3661 gcggttggtg aagatctggc acagcagcta cgtgacgaac tggaactggt gaaaggcgcg
     3721 tctaacgagt tcgacaaaga attgttcctt gcgggcgaaa tcactccggt attcttcggt
     3781 actgcgctgg gtaacttcgg cgttgatcat atgctggatg gcctggtgga gtgggctcct
     3841 gcgccgatgc cgcgtcagac tgatacccgt accgtagagg cgagcgaaga caaatttacc
     3901 ggcttcgtat ttaaaattca ggccaacatg gacccgaaac accgcgaccg cgtggcgttt
     3961 atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg
     4021 aaagatgtgg ttatctccga cgcgctgacc tttatggcgg gcgaccgttc gcacgttgaa
     4081 gaagcgtatc cgggcgatat cctcggcctg cacaaccacg gcactattca gatcggcgac
     4141 acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg
     4201 ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta
     4261 cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc
     4321 gttggtgctg ttggtgtgct gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac
     4381 agcgttgaag cagtatatga atcagtcaac gttgccactg cccgctgggt agaatgtgcg
     4441 gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc
     4501 ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt
     4561 tatcccgacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc agggcgcggt
     4621 agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg ttcttaaaac
     4681 attcactttt gcttatgttt tcgctgatat cccgagcggt ttcaaaattg tgatctatat
     4741 ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta gatatcagta
     4801 tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg ctgtaatggt
     4861 cgttattcat cacccatcgc ttttgtgatg gcgaccattg acttctgtag agggtgaagt
     4921 ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag caaacataca
     4981 ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg gctgtaatgt
     5041 tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag actaccaatg
     5101 aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc atggatgaca
     5161 gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc aagagcaccg
     5221 atatctctgt caaaaccgat caaaaagtcg tgaccctgag cggtttcgtt gaaagccagg
     5281 ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc tctgtcagcg
     5341 acaaactgca cgttcgcgac gctaaagaag gctctgtgaa gggctacgcg ggtgacaccg
     5401 ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct tcccgtcatg
     5461 tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtcgat tcccaggcac
     5521 agagcgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg
     5581 atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat
     5641 gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa gcggtgacat
     5701 taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg gttatcgcgt
     5761 taatcgccgc cgcacttggg tttggtggtc tggccggtac cgctgcaggc gcagctaaaa
     5821 ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc cgaaaacgac
     5881 cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga ctggcttttg
     5941 cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa aaacaggaag
     6001 gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg
     6061 ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg tggaattttc
     6121 acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga tctttatctc
     6181 ggcacatctg ccggggcgca gaacctctcg gcgtttatct gcaatcagcc cggttacgcg
     6241 cgcaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc
     6301 cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag ccagatgccg
     6361 ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta tatgtgcgcc
     6421 tgtcgtcagg atgactacgc gcctaattac tttttaccaa ccaaacaaaa ctggctggat
     6481 gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc gctggaaggc
     6541 attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag
     6601 ggcgctaaaa cgttggtcgt cattcgcact gtgccgtcac aaatgtacta cacgccgcag
     6661 tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct ggtcaatctg
     6721 gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa accaccgggc
     6781 aagctgcgga tattcgaaat ttatccgccg aagccattac atagtatcgc gcttggcagt
     6841 cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggtcg ttatttcctc
     6901 gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct ggtgccagtg
     6961 gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac gctggttgcc
     7021 gaagtgagcg atgctccgca ggcgaacgac ccgacattta acaatgagga tctggcttga
     7081 tttgccgttt tatcgatacc cactgccatt ttgatttccc gccgtttagt ggcgatgaag
     7141 aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag
     7201 aggcggaaaa ttttgcccgc gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg
     7261 cattggggtt gcatccgggg atgttggaaa aacatagcga tgtgtctctt gatcagctac
     7321 agcaggcgct ggaaaggcgt ccggcgaagg tggtggcggt gggggagatc ggtctggatc
     7381 tcttcggcga cgatccgcaa tttgagaggc agcagtggtt actcgacgaa cagctgaaac
     7441 tggcgaaacg ctacgatctg ccggtgatcc ttcattcccg gcgtatacac gacaaactgg
     7501 cgatgcatct taaacgtcac gatttaccgt gcactggcgt ggttcacggt ttttccggca
     7561 gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta ggcggtacta
     7621 tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt
     7681 tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg cagcctaacc
     7741 gaccggaaca ggctgtgcgg gtgtttgacg tgctttgcga gttgcgcccg gaaccagaag
     7801 atgagattgc cgaagtgttg cttaataata cgtatgcgtt gtttagcgtt agtgggtagc
     7861 ctacccttcg taggcagcat ccggcagtcg gcgcataatg cctgatgcga cgcttaacgc
     7921 gtcttatcat gcctac
//
LOCUS       DAZOYL010000186         7818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZOYL010000186 DAZOYL010000000
VERSION     DAZOYL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.187"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="ACHUOY_002901"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="ACHUOY_002901"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR0683626.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="ACHUOY_002902"
     CDS             complement(2362..2802)
                     /locus_tag="ACHUOY_002902"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0683627.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHUOY_002903"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHUOY_002903"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR0683628.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="ACHUOY_002904"
     CDS             3520..4218
                     /locus_tag="ACHUOY_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR0683629.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="ACHUOY_002905"
     CDS             4447..5655
                     /locus_tag="ACHUOY_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0683630.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="ACHUOY_002906"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="ACHUOY_002906"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR0683631.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
BASE COUNT         1790 a         1918 c         2076 g         2034 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttc
//
LOCUS       DAZOYL010000187         7795 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZOYL010000187 DAZOYL010000000
VERSION     DAZOYL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.188"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1962
                     /gene="acnA"
                     /locus_tag="ACHUOY_002907"
     CDS             <1..1962
                     /gene="acnA"
                     /locus_tag="ACHUOY_002907"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR0683632.1"
                     /translation="GQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVE
                     KFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELV
                     EKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKVFAASNE
                     LEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVI
                     LGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNSGPLPDP
                     IETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEP
                     IGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDT
                     YGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSP
                     AGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSD
                     VVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRS
                     NLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVP
                     CRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2026..2616)
                     /gene="ribA"
                     /locus_tag="ACHUOY_002908"
     CDS             complement(2026..2616)
                     /gene="ribA"
                     /locus_tag="ACHUOY_002908"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR0683633.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2786..3550
                     /gene="pgpB"
                     /locus_tag="ACHUOY_002909"
     CDS             2786..3550
                     /gene="pgpB"
                     /locus_tag="ACHUOY_002909"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR0683634.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3699..4007
                     /gene="lapA"
                     /locus_tag="ACHUOY_002910"
     CDS             3699..4007
                     /gene="lapA"
                     /locus_tag="ACHUOY_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR0683635.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4014..5183
                     /gene="lapB"
                     /locus_tag="ACHUOY_002911"
     CDS             4014..5183
                     /gene="lapB"
                     /locus_tag="ACHUOY_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR0683636.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5376..6113
                     /gene="pyrF"
                     /locus_tag="ACHUOY_002912"
     CDS             5376..6113
                     /gene="pyrF"
                     /locus_tag="ACHUOY_002912"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR0683637.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6113..6439
                     /gene="yciH"
                     /locus_tag="ACHUOY_002913"
     CDS             6113..6439
                     /gene="yciH"
                     /locus_tag="ACHUOY_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR0683638.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(6565..6783)
                     /gene="osmB"
                     /locus_tag="ACHUOY_002914"
     CDS             complement(6565..6783)
                     /gene="osmB"
                     /locus_tag="ACHUOY_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR0683639.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(7052..>7795)
                     /locus_tag="ACHUOY_002915"
     CDS             complement(7052..>7795)
                     /locus_tag="ACHUOY_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR0683640.1"
                     /translation="SRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKLSY
                     LRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLART
                     LGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHFSK
                     AFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVERF
                     NRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         1925 a         1833 c         2058 g         1979 t
ORIGIN      
        1 ggccagccgg tttccatgct tatcccggac gtagtgggct ttaaacttac cggtaaatta
       61 cgtgaaggta ttaccgccac agacctggtt ctcactgtta cccaaatgct gcgcaaacat
      121 ggcgtggtgg agaaattcgt cgaattttat ggtgatggtc tggattcact accgctggcg
      181 gatcgcgcca ccattgccaa tatgtcgcca gaatatggtg ccacctgtgg cttcttccca
      241 atcgatgctg taaccctcga ttacatgcgt ttaagcgggc gcagcgaaga tcaggttgag
      301 ttggtcgaaa aatatgccaa agcgcagggc atgtggcgta acccgggcga tgaaccgatt
      361 tttaccagta cgttagaact ggatatgaat gacgttgaag cgagcctggc agggccaaaa
      421 cgcccacagg atcgcgttgc actgcccgat gtaccaaaag tatttgccgc cagtaacgaa
      481 ctggaagtga atgccacgca taaagatcgc cagccggtcg attatgttat gaacggacat
      541 cagtatcagt tacctgatgg cgctgtggtc attgctgcga taacctcgtg caccaacacc
      601 tctaacccaa gtgtgctgat ggctgcaggc ttgctggcga aaaaagccgt aattcttggc
      661 ctcaagcggc aaccgtgggt caaagcgtcg ctggcaccag gctcgaaagt tgtttctgac
      721 tatctggcaa aagcgaaact gacaccgttt ctcgacgaac tggggtttaa ccttgtggga
      781 tacggttgta ctacctgtat tggtaactct gggccgctgc ccgatcctat cgaaacggca
      841 atcaaaaaag gcgatttaac cgtcggtgca gtgctgtccg gcaaccgtaa ctttgaaggc
      901 cgtatccatc cgctggttaa aaccaactgg ctggcctcgc cgccgctggt ggttgcctat
      961 gcgctggcgg gaaatatgaa tatcaacctg gcttctgagc ctattggtca tgatcgtaaa
     1021 ggcgatccgg tttatctgaa agatatctgg ccatcggcac aagaaattgc ccgtgcggta
     1081 gaacaagtct ccacagaaat gttccgcaaa gagtacgcag aagtttttga aggcacagca
     1141 gagtggaagg aaattaacgt cacacgatcc gatacctacg gttggcagga ggactcaacc
     1201 tatattcgct tatcgccttt ctttgatgaa atgcaggcaa caccagcacc agtggaagat
     1261 attcacggtg cgcggatcct cgcaatgctg ggggattcag tcaccactga ccatatctct
     1321 ccggcgggca gtattaagcc cgacagccca gcgggtcgat atctacaagg tcggggtgtt
     1381 gagcgaaaag actttaactc ctacggttcg cggcgtggta accatgaagt gatgatgcgc
     1441 ggcaccttcg ccaatattcg catccgtaat gaaatggtgc ctggcgttga aggggggatg
     1501 acgcggcatt tacctgacag cgacgtagtc tctatttatg atgctgcgat gcgctataag
     1561 caggaacaaa cgccgctggc ggtgattgcc gggaaagagt atggttcagg ctcaagccgt
     1621 gactgggcgg caaaaggtcc tcgtctgctt ggaattcgtg tggtgattgc cgaatcgttt
     1681 gaacgaattc accgttcgaa tttaattggc atgggcatcc tgccgctgga atttccgcaa
     1741 ggcgtaacgc gtaaaacgtt aggactaacc ggggaagaga agattgatat tggcgatctg
     1801 caaaatctac aacccggtgc gacggttccg gtgacgctta cgcgcgcgga tggtagccag
     1861 gaggtcgtac cctgccgttg tcgtatcgac accgcgacgg agttgaccta ctaccagaac
     1921 gacggcattt tgcattatgt cattcgtaat atgctgaagt aacaaatatt tgcttgccgg
     1981 ttattttgct tccggcaagc aaattaattc cacaatgcga gagggttatt tgttcagcaa
     2041 atgacccatt ttctcggctt tggtatcgag ataatgttcg ttattggggt tacgacctac
     2101 aatcaatggt acgcgttcaa caatattaat ccctgcttcg gtcagaattt cgactttttt
     2161 cgggttattg gttaacaagc ggacttcatt gacgccaagg agtttgaaca tatcagcgca
     2221 aagagtgaag tcgcgctcat cagcggcgaa gcctaactga tggttagcct ctacggtatc
     2281 gtaaccttga tcctgcagtg cgtaagcgcg gattttattc agcagaccaa tgttacgacc
     2341 ttcctgacgg tgatacagca aaataccacg gccttcctcg gcaatttgcg tcaatgccgc
     2401 ttcgagctgg aagccacaat cgcagcgcaa gctgaacagg gcgtcaccgg tcagacattc
     2461 ggaatggacg cgcgcaagta ccggggtatg cccggaaata tcgccataga ctagcgcgac
     2521 atgatcgtgt ccggttgcca gttcttcaaa tcccaccatc aggaaatcgc cccatggggt
     2581 tggcagtttg gcttctgcca cacgtttaag ctgcatgaaa ttctccagat aatgctggtt
     2641 ctgtattggc ttattttgcc ataacgagaa ggggttcacc taatcacgac gcgtcgatcg
     2701 ttcacggaat ggcacaaatc tgtcaatttt tcctggaact ggcgttttca gttatgattg
     2761 tgggacttat caaaaaggag aggccatgcg ttcgattgcc agacgtaccg cagtgggagc
     2821 tgcactattg cttgtcatgc cagtagccgt atggatttct ggctggcgtt ggcaacctgg
     2881 agaacaaagt tggctactaa aagcggcttt ttgggttact gaaactgtca cccagccctg
     2941 gggcgtcatt acacatttga ttttattcgg ctggtttctc tggtgtctgc gtttttgcat
     3001 taaggctgcc tttgtattat ttgccattct ggcggccgta atcattgtgg gacaaggcgt
     3061 taaatcctgg atcaaagaca aagtccagga accacgacct tttgttatct ggctggaaaa
     3121 aacacatcat attccggttg atgagttcta cactttaaag cgagcagaac gcggaaatct
     3181 agtgaaagaa cagttggctg aagagaaaaa tatcccacaa tatttgcgtt cacactggca
     3241 gaaagagacg gggtttgcct ttccttccgg tcacacgatg tttgctgcca gttgggcact
     3301 gctggccgtt ggtttgctgt ggccgcgtcg gcgaacgtta accattgcta tcttgctggt
     3361 ctgggcaacg ggagtcatgg gaagccgcct gctgctcggg atgcattggc cacgcgatct
     3421 ggtagtagct acgttgattt cgtgggcgct ggtggcggtg gcaacgtggc ttgcgcaacg
     3481 aatttgtggg ccattaacac cacctgcgga agaaaatcgc gaaatagcgc aacgagaaca
     3541 agaaagttaa cgctggttga ttttccgaat ttagccctta aatcatcaac aatgcgcgtg
     3601 gatgccattt cgcagacggc gcgaaaatgg tactttaaag ggctattgcg gtaagttgac
     3661 cataatttat tcgctttaac cacataacgg gaagtaatgt gaaatattta ctcattttct
     3721 tactggtgtt agcgatcttc gtgatttcgg tcacgttggg tgcgcagaac gatcaacagg
     3781 tgacgtttaa ttatctgtta gcgcaagggg agtaccgtat ttccacattg ctggcggtat
     3841 tgtttgctgc gggggttgct atcggttggt tgatttgtgg cctgttctgg ctgcgagttc
     3901 gtgtttccct ggcgcgcgct gaacgtaaaa taaagcgact ggaaaaccag ctttcacccg
     3961 cgactgacgt ggctgtagtg ccgcactcgt cagcggcgaa ggaataactt tctatgctgg
     4021 agttgttgtt tctgcttttg cctgtagccg ctgcctatgg ctggtatatg ggccgcagaa
     4081 gtgcgcaaca aaacaagcaa gatgaagcca accgcttgtc gcgtgattac gtagcggggg
     4141 ttaacttcct gcttagtaat caacaggata aagcggtaga cctgtttctc gatatgctta
     4201 aagaggatac aggcaccgtt gaagcccacc ttacgctcgg aaacctgttc cgttcgcgtg
     4261 gcgaagttga tcgcgctatt cgcatccatc agaccctaat ggaaagcgcc tcactgacct
     4321 atgaacagcg tctgttggcg attcaacaac tggggcgtga ttacatggcc gccgggttat
     4381 acgaccgcgc ggaagacatg ttcaatcagc tgaccgatga aactgacttc cgcattggcg
     4441 cgctgcaaca gttgctacaa atctaccagg ctaccagcga gtggcagaaa gcaattgatg
     4501 ttgccgaacg cctggtgaag ctgggtaaag ataaacagcg cgtcgaaatt gcccatttct
     4561 actgtgagtt agccctgcag catatggcca gcgacgatct cgatcgtgcc atgaccttgc
     4621 taaaaaaagg ggcggcggca gataaaaaca gcgcccgcgt atccatcatg atgggacgcg
     4681 tgtttatggc gaaaggagaa tacgccaaag ccgtcgaaag tttgcaacgc gtgatatccc
     4741 aggacagaga actggtcagc gaaacgctgg aaatgttgca aacctgctat cagcagttgg
     4801 gtaaaactgc cgaatgggca gaattcctgc aacgcgcggt ggaagagaac accggtgccg
     4861 atgctgaatt gatgcttgct gatatcatcg aagcgcgcga cggtagtgag gccgcacagg
     4921 tctatattac gcgccagctt cagcgtcatc cgaccatgcg tgtgttccat aagttaatgg
     4981 attaccactt aaatgaagcg gaagaagggc gtgccaaaga gagcctgatg gtgctgcgtg
     5041 acatggttgg cgaaaaggta cgtagtaagc ctcgttatcg ctgccagaaa tgtggtttta
     5101 ccgcatacac cctctactgg cattgtccgt cttgtcgggc ctggtcaacc attaaaccga
     5161 ttcgcggtct tgatggcctg taatttttaa aaaaatccga ctttagttac aacatactag
     5221 ttattgatgt tccattgtgc tccggcaacg acggagcaga aaaagcctgc caggggaaaa
     5281 atcgcaactg ttaatttttt atttccacgg gtagaatgct cgccgtttac ctgtttcgcg
     5341 ccacttccgg tgcccatcat caagaaggtc tggtcatgac gttaactgct tcatcttctt
     5401 cccgcgctgt tacgaattct cctgtggttg ttgcccttga ttatcataat cgtgatgacg
     5461 cgctgtcctt tgtcgacaag atcgacccac gcgattgtcg tctgaaggtc ggcaaagaga
     5521 tgtttacatt gtttgggcca cagtttgtgc gcgaacttca acagcgtggt tttgatatct
     5581 ttcttgacct gaaattccac gatattccta acactgcagc gcacgctgtc gctgctgcag
     5641 ctgacttagg cgtgtggatg gtgaatgttc atgcctctgg tggggcgcgt atgatgaccg
     5701 cagcgcgtga ggcactggtt ccgtttggca aagatgcacc gcttttgatt gctgtgacag
     5761 tgttgaccag catggaagcc agcgacctgg tcgatcttgg catgacactg tcacctgcag
     5821 attatgcaga acgtctggcg gcactgacgc aaaaatgtgg ccttgatggt gtggtgtgtt
     5881 ctgctcagga agctgtgcgc tttaaacagg tattcggtca ggagttcaaa ctggttacgc
     5941 cgggcattcg tccgcagggg agtgaagctg gtgaccagcg ccgcattatg acgccagaac
     6001 aggcgttgtc ggctggtgtt gattatatgg tgattggtcg cccggtaacg caatcggtag
     6061 atccagcgca gacgctgaaa gcgatcaacg cctctttaca gcggagtgca tgatgagtga
     6121 ttccaacagc cgtctggtct actcaacgga aaccggacgt attgatgagc ccaaagcggc
     6181 ccctgtacgc cctaaaggcg acggtgtggt gcgtattcag cgtcagacca gtggacgtaa
     6241 aggtaagggc gtttgcctga ttaccggtgt cgatctcgat gatgccgaac tgacaaaact
     6301 tgcagcggaa ctgaagaaaa aatgcggctg cggcggagca gttaaagatg gagttattga
     6361 aatccagggc gataagcgtg atttattaaa atcactactt gaagcgaaag ggatgaaggt
     6421 aaaactcgca ggcggttaac ataaaaagcc acggatatat ccgtggcttt cgaatatttt
     6481 actgtgcgta ttattcagtt atttcgtact gaacagaatc aagcgtatta taattgcgag
     6541 tcgataatcg acatttattt acgattattt accgacctgg tgaccaataa caccaccgac
     6601 agctgcacca cctaatgtac ccaacgtact gccatcggtc agtactgcac cgcctaatgc
     6661 ccctgcaccc gcgccgattg cggtgttgcg gtcccgttta gaccagttag aacaggcact
     6721 cagagacatt gccaaagtaa ttgccagaac agccgcggtc atttttttgc tcgttacaaa
     6781 cataatactc tctcctgaat ttatgattca cggaagtaag ctctctataa ctataatagc
     6841 taagaataag cctggtgaaa ttattccgtg aaatctgctc gcgcgcaggg ttatatcacg
     6901 caggtgataa tgacttcctg ttatatcgct gataataatt ttatatctta agcgtgttaa
     6961 taacaggtaa atagtcttaa ttatcaacca ggaatcatct tagagcggat gatttgccaa
     7021 actgcaaatc atccgtagag aagggaaatg gttaaatgtc aacgacgtga acggtgagtt
     7081 tactgtgctc aagatgcatc agatcgctgg cgcgtatttt cgaatcggta atcacgcgat
     7141 tgaatcgctc aacgggaccg atggagtatg aatgtacagc accaaatttc gagctgtcag
     7201 tcaggactat cgcttcgcac tctttttcca gcacggcatt gaccacatcg gtacgcatca
     7261 tatcgcgacc ggtgaatcca gtttcaggtt gccagccatc aataccaata aatgctttgc
     7321 tgaaatgcac ctgttggatg cactggcgtg tcaacgggcc aaccatactt tcgctttttt
     7381 tctggtacac gccaccgagc aaaatgactt cacaaggcgc gtctttcagc aaatgcgcga
     7441 tgtagctgct gaccgtgatg atagtgacat ttttcttctg ctcccccaga gtccgggcga
     7501 gcagggcatt gctgctgcca ttttcgataa agatggtttc gcccggttga accagtgacg
     7561 cggcaaactc ggcgagttca cgcttcagcg tatagttgct catcatacgg gtttcgacgt
     7621 catcactatc aagcgaaact gcaaagccat gtgcacggcg gaggtaactc agtttttcga
     7681 gggtgttgag atcctggcga atggtaactt cagaaactcc agtggctttt gccagatcgg
     7741 ttacgctaac ctgaccctgg tcaatgacca tctgtagaat agtttgttgt cggga
//
LOCUS       DAZOYL010000188         7776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZOYL010000188 DAZOYL010000000
VERSION     DAZOYL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.189"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHUOY_002916"
     CDS             complement(<1..1780)
                     /locus_tag="ACHUOY_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR0683641.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPEWLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUOY_002917"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUOY_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0683642.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUOY_002918"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUOY_002918"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0683643.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUOY_002919"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUOY_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR0683644.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUOY_002920"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUOY_002920"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR0683645.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHUOY_002921"
     CDS             complement(5968..7005)
                     /locus_tag="ACHUOY_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0683646.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHUOY_002922"
     CDS             7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHUOY_002922"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0683647.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc attcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaag ccagcacccg
     7141 gctggctaag taatactgga agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag
     7201 ggtcttcctg atcgcgagac aataataatc attctcattc gcacttgtcc aacacttttt
     7261 gcaaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg gacatttacc
     7321 ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg
     7381 aggccatcag gcagattcac gcccagccgg aggtgtattg caacacacta caggtgcctc
     7441 atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc aagcaactcg
     7501 tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa cagcacccac
     7561 gccgcagtca tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg
     7621 tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg cgggtagatc
     7681 gcattgaact aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg
     7741 gctttgaaat tgaagggact ggtaagaagt acgcat
//
LOCUS       DAZOYL010000189         7715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZOYL010000189 DAZOYL010000000
VERSION     DAZOYL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.190"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHUOY_002923"
     CDS             <1..643
                     /locus_tag="ACHUOY_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0683648.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUOY_002924"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUOY_002924"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR0683649.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHUOY_002925"
     CDS             2474..2986
                     /locus_tag="ACHUOY_002925"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR0683650.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUOY_002926"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUOY_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR0683651.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHUOY_002927"
     CDS             5579..5782
                     /locus_tag="ACHUOY_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0683652.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUOY_002928"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUOY_002928"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR0683653.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="ACHUOY_002929"
     CDS             complement(6827..>7715)
                     /locus_tag="ACHUOY_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR0683654.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1785 a         2101 c         2020 g         1809 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg gacgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAZOYL010000190         7704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZOYL010000190 DAZOYL010000000
VERSION     DAZOYL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.191"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /locus_tag="ACHUOY_002930"
     CDS             complement(<1..611)
                     /locus_tag="ACHUOY_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR0683655.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHUOY_002931"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHUOY_002931"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0683656.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHUOY_002932"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHUOY_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR0683657.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHUOY_002933"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHUOY_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR0683658.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /locus_tag="ACHUOY_002934"
     CDS             complement(5085..6716)
                     /locus_tag="ACHUOY_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR0683659.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHUOY_002935"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHUOY_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR0683660.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="ACHUOY_002936"
     CDS             7139..7411
                     /locus_tag="ACHUOY_002936"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0683661.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..>7704
                     /locus_tag="ACHUOY_002937"
     CDS             7638..>7704
                     /locus_tag="ACHUOY_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683662.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSS"
BASE COUNT         1910 a         1865 c         1984 g         1945 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcagga gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggtgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcag
//
LOCUS       DAZOYL010000191         7667 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZOYL010000191 DAZOYL010000000
VERSION     DAZOYL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.192"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            131..634
                     /gene="dps"
                     /locus_tag="ACHUOY_002938"
                     /gene_synonym="pexB"
     CDS             131..634
                     /gene="dps"
                     /locus_tag="ACHUOY_002938"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR0683663.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1038..1784
                     /gene="glnH"
                     /locus_tag="ACHUOY_002939"
     CDS             1038..1784
                     /gene="glnH"
                     /locus_tag="ACHUOY_002939"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR0683664.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1923..2582
                     /gene="glnP"
                     /locus_tag="ACHUOY_002940"
     CDS             1923..2582
                     /gene="glnP"
                     /locus_tag="ACHUOY_002940"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR0683665.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2579..3301
                     /gene="glnQ"
                     /locus_tag="ACHUOY_002941"
     CDS             2579..3301
                     /gene="glnQ"
                     /locus_tag="ACHUOY_002941"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR0683666.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3418..5643
                     /gene="ybiO"
                     /locus_tag="ACHUOY_002942"
     CDS             3418..5643
                     /gene="ybiO"
                     /locus_tag="ACHUOY_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR0683667.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(5640..6566)
                     /gene="rlmF"
                     /locus_tag="ACHUOY_002943"
     CDS             complement(5640..6566)
                     /gene="rlmF"
                     /locus_tag="ACHUOY_002943"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR0683668.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6842..7102
                     /gene="mcbA"
                     /locus_tag="ACHUOY_002944"
     CDS             6842..7102
                     /gene="mcbA"
                     /locus_tag="ACHUOY_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR0683669.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7367..>7667
                     /locus_tag="ACHUOY_002945"
     CDS             7367..>7667
                     /locus_tag="ACHUOY_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0683670.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         1853 a         1962 c         1934 g         1918 t
ORIGIN      
        1 attagttttg tttttcacgc ttgttaccac tattagtgtg ataggaacag ccagaatagc
       61 ggaacacata gccggtgcta tacttagtct cgttaattac tgggacataa catcaagagg
      121 atatgaaatt atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc tgctttatac
      181 ccgcaacgat gtctccgaca gcgagaaaaa agcaacagta gagttgctga atcgccaggt
      241 tatccagttt attgatcttt ctttgattac caaacaagcg cactggaaca tgcgcggcgc
      301 taacttcatt gccgtacatg aaatgctgga tggcttccgc accgcactga tcgatcatct
      361 ggataccatg gcagaacgtg cagtgcagct gggcggtgta gctctgggga ccactcaagt
      421 tatcaacagc aaaaccccgc tgaaaagtta cccgctggac atccacaacg ttcaggatca
      481 cctgaaagaa ctggctgacc gttacgcaat cgtcgctaat gacgtacgca aagcgattgg
      541 cgaagcgaaa gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg acctggataa
      601 attcctgtgg tttatcgagt ctaacatcga ataaatccat cgctgatggt gcagaacttt
      661 agtacccgat aaaagcggct tcctgacagg aggccgtttt gttttgcagc ccacctcaac
      721 gcacttattt agtgcatcca tctgctatct ccagctgatt aagtaaattt tttgtatcca
      781 catcatcaca caatcgttac ataaagattg ttttttcatc aggttttacg ctaaataatc
      841 actgtgttga gtgcacaatt ttagcgcacc agattggtgc cccagaatgg tgcatcttca
      901 gggtattgcc ctataaatcg tgcatcacgt ttttgccgca tctcgaaaaa tcaaggagtt
      961 gcaaaactgg cacgattttt tcatatatgt gaatgtcacg caggggatcg tcccgtggat
     1021 agaaaaaagg aaatgctatg aagtctgtat taaaagtttc actggctgca ctgaccctgg
     1081 cttttgcggt ttcttctcat gccgcggata aaaaattagt tgtcgcgacg gataccgcct
     1141 tcgttccgtt tgaatttaaa cagggcgata aatatgtggg ctttgacgtt gatctgtggg
     1201 ctgccatcgc taaagagctg aagctggatt acgaactgaa gccgatggat ttcagtggga
     1261 tcattccggc actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt accatcaccg
     1321 acgagcgtaa aaaagcgatc gatttctctg acggctacta caaaagcggc ctgttagtga
     1381 tggtgaaagc taacaataac gatgtgaaaa gcgtgaaaga tctcgacggg aaagtggttg
     1441 ctgttaagag cggtactggc tccgttgatt acgcgaaagc aaacatcaaa actaaagatc
     1501 tgcgtcagtt cccgaacatc gataacgcct atatggaact gggcaccaac cgcgcagacg
     1561 ccgttctgca cgatacgcca aacattctgt acttcatcaa aaccgccggt aacggtcagt
     1621 tcaaagcggt aggtgactct ctggaagcgc agcaatacgg tattgcgttc ccgaaaggta
     1681 gcgacgagct gcgtgacaaa gtcaacggcg cgttgaaaac cctgcgcgag aacggaactt
     1741 acaacgaaat ctacaaaaaa tggttcggta ctgaaccgaa ataataacgc tacacctgta
     1801 aagcgcactg gcagttccct ctcccctctg gggagaggat tagggtgagg ggcgcaaacc
     1861 cgctccgggg ccattaatta ccctgaattt gattatttac accacggtaa caggaacaac
     1921 atatgcagtt tgactggagt gccatctggc ctgccattcc gcttctgatt gaaggtgcca
     1981 aaatgaccct gtggatttcg gtcctcggtc tggctggcgg tctggtaatc ggattgctgg
     2041 caggttttgc acgcaccttc ggtggttgga tagccaacca cgtcgcgctg gtctttattg
     2101 aggtgatccg cggcacacct atcgtcgtcc aggtgatgtt tatttatttc gccctgccga
     2161 tggcgtttaa cgacttacgc atcgacccat ttactgcggc ggtggtcacc atcatgatca
     2221 actccggcgc gtacattgcg gaaatcacgc gtggtgcggt gctgtctatt cacaaaggtt
     2281 ttcgtgaagc aggactggcg ctcggtcttt cacgttggga aaccattcgc tacgtcattt
     2341 taccgctggc actgcgtcgt atgctgccgc cgctgggtaa ccagtggatc atcagcatta
     2401 aagacacctc gctgtttatt gtgatcggcg tggcggaact gacccgtcag gggcaagaaa
     2461 ttattgccgg taacttccgc gcccttgaga tctggagcgc cgtggcggtg ttctatctga
     2521 ttattaccct ggtgctaagc tttattttgc gtcgtctgga aagaaggatg aaaatcctgt
     2581 gattgaattt aaaaacgtct ccaagcactt tggcccaacc caggtgcttc acaatatcga
     2641 tttgaacatt gcccagggcg aagtcgtggt gattatcggg ccgtccggtt ccggtaaatc
     2701 gaccctgctg cgctgcatca acaaactgga agaaatcacc tccggcgatc tgattgtcga
     2761 tggcctgaag gttaacgatc cgaaagttga cgagcgcctg attcgccagg aagcaggtat
     2821 ggtgttccag cagttttacc tcttcccgca tctgacggcg ctggaaaacg tcatgtttgg
     2881 tccgctacgc gtgcgtggcg cgaacaaaga agaggcggaa aaactggcgc gtgagctgct
     2941 ggcgaaagtc ggtctggcag aacgtgcaca tcactaccct tccgaacttt ctggtggtca
     3001 acagcagcgt gtggcgattg cccgcgcgct ggcggtgaag ccgaaaatga tgctgtttga
     3061 tgaaccgact tccgctcttg acccggaact gcgccatgaa gtgctgaagg ttatgcagga
     3121 tctggctgaa gaagggatga cgatggtgat cgtgacccac gaaatcggtt ttgccgagaa
     3181 agtagcttcg cggctgatct ttatcgacaa aggccggatt gcggaagatg gcgatccgca
     3241 ggtattgatc aagaacccgc cgagccagcg cttgcaggaa tttttgcagc acgtctctta
     3301 acttctctgc ctccctttca cccgaaaggg aggcacacca gattcctctc atttaaaatc
     3361 gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg aggagtcatg
     3421 cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc ggtatccata
     3481 cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc ggaaccggat
     3541 atcgaacaaa aaaaagcggc ctatggcgca ctggcggatg tgctggataa cgacacctcg
     3601 cgtaaagaat tgatcgacca gttgcgtact gtcgccgcga cgccccctgc ggaaccggtc
     3661 ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg tgctgcaaaa ggtcaccgaa
     3721 gtcagccgcc attatggtga agccctttcc gcccgcttcg ggcaacttta tcgcaatatc
     3781 accggctccc cgcataagcc atttaatcca caaaccttca gcaatgcgct gagccatttt
     3841 tcaatgttag cggtattagt gtttggtttt tactggctga ttcgcctgtg cgcactgccg
     3901 ctgtatcgca aaatgggtca gtgggcgagg caaaaaaatc gtgagcgcag caactggttg
     3961 cagcttccgg cgatgattat cggggcgttt attatcgacc tgctgttact ggccctgaca
     4021 ttatttgtcg gccaggtatt aagcgacaac ctgaatgcag gcagtcgcac catcgctttc
     4081 caacaaagtt tgtttctcaa cgcctttgcc ctgattgaat ttttcaaagc cgtactacgc
     4141 ctgatttttt gcccaaacgt ggcggagctg cgcccgttca cgattcagga cgagagcgcc
     4201 cgttactgga gtcgtcgcct gagctggtta agcagtttga taggttatgg cctgattgtg
     4261 gccgtgccga ttatctctaa tcaggtgaat gtacagatag gtgcgctggc gaacgtcatc
     4321 attatgctgt gcatgaccgt ctgggcgttg tacctgatct ttcgtaataa aaaagagatt
     4381 acccagcatt tgctcaactt tgcggagcat tcgctggcct ttttcagcct gtttatccgc
     4441 gcctttgcgc tggtgtggca ctggctggca agcgcctatt ttatcgtgct gtttttcttt
     4501 tcgttgttcg atccgggcaa cagcctgaaa tttatgatgg gtgcaacggt gcgcagcctg
     4561 gcgattattg gtatcgcagc gtttgtttcc ggtatgtttt cccgctggct ggcaaaaacc
     4621 atcactctct cgccacatac tcagcgtaac tatccagagt tgcaaaaacg gctgaatggc
     4681 tggctttcgg cggcgctgaa aacggcgcgt attctgaccg tctgcgtggc ggtaatgttg
     4741 ctgttgagcg catggggatt gttcgatttc tggaactggc tgcaaaacgg cgcggggcag
     4801 aaaaccgtag atatcctgat ccgtatcgca cttattcttt ttttctcggc ggttggctgg
     4861 acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg atattcatgg ccgcccgcta
     4921 cccagcgccc gtacgcgtac cctgctgacg ctgttccgta acgcgctggc ggtgattatc
     4981 agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg tcaatatcgc gccattgctg
     5041 gcaggtgccg gggcattagg tctggctatc tcgtttggtt cgcaaacgct ggtgaaagat
     5101 atcattaccg gggtgtttat tcagtttgaa aacggcatga acactggaga tttggtgact
     5161 atcgggccgt tgaccggcac agtggaacgg atgtcgattc gctccgtggg cgtgcgccag
     5221 gataccgggg cgtatcacat cattccgtgg tcttcgataa ccacctttgc taacttcgtc
     5281 cgcggtattg gttcggtagt ggcaaactac gatgttgatc gccatgaaga tgctgataaa
     5341 gccaatcagg cactgaaaga tgcggtagcg gaattaatgg aaaacgaaga aattcgcggg
     5401 ctgattattg gtgaaccgaa ttttgccggg attgtcggct taagcaatac cgcgtttaca
     5461 ctgcgtgttt cgttcaccac gctgccactc aaacagtgga cggtacgctt tgccctcgac
     5521 agccaggtga aaaaacattt cgacctggcg ggcgttcgcg cgccagtgca gacttatcag
     5581 gtgctgccta ctccgggcgc gaccccggct gaaccgttac cgccggggga gccaacgctt
     5641 taacgctggc gattgacaaa acggcggcgc tgctcgtcgt tcataaaggt ccaggcaata
     5701 aagcgactct gcttttgccc ctgggccatc tcttttttta ccaccttcac cgcgcccacg
     5761 tcggtcagag cacgatacaa cggcggtaag ttttcaccac gagataccag cgaggtaaac
     5821 cacatcacct gcttcgcgaa gcctttgctc tcttcaatca tctttttgat aaaggcgact
     5881 tcaccacctt cacaccacaa ctcctgttgc tgaccaccaa agttcagtgc atcgtctttg
     5941 ttcagcccca ggttacggcg tttacgctca ctacctgccc gcgctgcagc ggcggaatcg
     6001 tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt ttttatggat gatgccgtta
     6061 aaaattgccc cactctcttt ttgccgacgc agacgaatgg cgcggttaag ccccggatta
     6121 gcactgataa tcgcctgcgc actgcttaac gcctggctgc tggtttcact accggtaaaa
     6181 cgccagccat attcatgtac gccaatcagc ggataaatac agttcgcacc aacgccgata
     6241 tccagaatgc tggcattcgc tggaattgtt ccgcttgctt cagccagtaa atcggcaagg
     6301 tgatgaatat aatccgcccg gcccggtact ggtgggcaga gaaaaccgtc ggggatatcc
     6361 cagttcgcta cggcgtaaaa atgggccagc aacgccttat tgagcgcctt caccgccagc
     6421 ggattggcaa agtctacgct ttgctccccg gcgggtgtaa gcgtgaggaa ttgcctgagt
     6481 tcaggattga cctgacaaag cgtggcgaga tcgtagcggc tgtgatgacg gttgcgcgga
     6541 tgcaaccccg gtttctgggc ggacatagca ttctccttta agcagcggcg taagataccc
     6601 gcataacgcg acagggtaaa tatctggctg cgtttatcat tccccaaaac cttaacatgg
     6661 ctatggttta tggcaattca tcataaaaat cagcattatg catgtcatta atttgtctta
     6721 ttttgtaaaa atatatcgtt caaaaaataa gcacatctct gtcaattttt tgcgtcattt
     6781 gcatcgcgca ggcgacctac acttatcagg cactacccgt tgtatttatg aggatgtcat
     6841 tatgaaaaag tgcctcacac tactgattgc caccgtcctg agcggaatct ctctcacggc
     6901 ttatgccgcg caaccgatga gtaacctgga cagcggtcag ctacggcccg ccggtaccgt
     6961 ttcggcaact ggcgcatcaa acctaagcga tctggaggat aaactggcag aaaaagcgcg
     7021 cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc agatgttcgg
     7081 tactgcaacc atctacaaat aaccccacca acgtaactgc attcgcctct ggatgcagtt
     7141 ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaactc aatgtaaata aatgtatttc
     7201 tttttcgcgc aatgggtgat agaaaatcgc gccaagtgat aatgcttatc aaaattatta
     7261 tcacttgcat gaacactatc acgggattaa cagtggcatc gcatccgcag agatgctttc
     7321 tcgtggcagt gaaaatttca acatataaga aaaagtcacc tgcaaaatgg aaaacaatcg
     7381 caatttccct gccagacaat ttcattcgct cacgttcttt gccggtcttt gtattggcat
     7441 cacgcctgtg gctcaggcac tcgccgccga agggcaaact aacgcggatg acacgctggt
     7501 tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa tctgccgatc cgaaattctc
     7561 gcgtccggta gcggatacta cccgcacgat gacggtgatt tctgaacaag tgattaaaga
     7621 tcagggcgca accaacctta ccgacgcgct caaaaacgcc cccggcg
//
LOCUS       DAZOYL010000192         7653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZOYL010000192 DAZOYL010000000
VERSION     DAZOYL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7653
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.193"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..859
                     /locus_tag="ACHUOY_002946"
     CDS             <1..859
                     /locus_tag="ACHUOY_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0683671.1"
                     /translation="AVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRS
                     VLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLS
                     LFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMI
                     PHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAM
                     TIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPASRNF
                     RIHLLNALR"
     gene            994..2214
                     /gene="mlc"
                     /locus_tag="ACHUOY_002947"
                     /gene_synonym="dgsA"
     CDS             994..2214
                     /gene="mlc"
                     /locus_tag="ACHUOY_002947"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR0683672.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2339..3034
                     /gene="bioD"
                     /locus_tag="ACHUOY_002948"
     CDS             2339..3034
                     /gene="bioD"
                     /locus_tag="ACHUOY_002948"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0683673.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(2987..4279)
                     /gene="clcB"
                     /locus_tag="ACHUOY_002949"
     CDS             complement(2987..4279)
                     /gene="clcB"
                     /locus_tag="ACHUOY_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR0683674.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4438..5052)
                     /gene="dmsD"
                     /locus_tag="ACHUOY_002950"
     CDS             complement(4438..5052)
                     /gene="dmsD"
                     /locus_tag="ACHUOY_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR0683675.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5095..5949)
                     /gene="ynfH"
                     /locus_tag="ACHUOY_002951"
     CDS             complement(5095..5949)
                     /gene="ynfH"
                     /locus_tag="ACHUOY_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR0683676.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(5951..6568)
                     /gene="ynfG"
                     /locus_tag="ACHUOY_002952"
     CDS             complement(5951..6568)
                     /gene="ynfG"
                     /locus_tag="ACHUOY_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR0683677.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(6579..>7653)
                     /locus_tag="ACHUOY_002953"
     CDS             complement(6579..>7653)
                     /locus_tag="ACHUOY_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR0683678.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         1932 a         1962 c         2005 g         1754 t
ORIGIN      
        1 tgctgttgcg gaagagctgc atttcgggcg cgccgctgcc cgcctgaata tttcgcaacc
       61 accgctaagt cagcagattc aggcactgga gcaacaaatt ggtgcccgac tgctggcacg
      121 aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag atagtcggca
      181 aatcctgtct atggtagatg acgctgccgc tcgcgctgaa agactgcatc agggtgaagc
      241 gggggagttg cgcattggtt ttacttcgtc cgctcctttt attcgggcgg tgtccgatac
      301 gttatcgctg tttcgccgtg attatcccga tgtccattta caaacccgcg aaatgaacac
      361 tcgtgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc tgcgtaacac
      421 agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta tggcgatgat
      481 cccgcacgat catcccctgg caaataaccc gaatgtaacg ctggctgaac tggcgaaaga
      541 acccttcgtc ttttttgatc cgcacgtcgg gacagggctg tatgacgata ttctcgggct
      601 gatgcgacgt taccatttga cgcccgtcat cactcaggag gtgggcgagg caatgaccat
      661 catcggtctg gtttccgccg gtctgggtgt ttcaattttg cctgcttcat ttaaacgtgt
      721 tcagctcaac gaaatgcgct gggtgccgat tgctgaagag gatgcggttt ctgaaatgtg
      781 gttggtctgg ccgaaacatc atgaacaaag tccagcttcg cgtaactttc gtattcatct
      841 gctgaatgct ctcaggtgag ggaaatttca gcgaaaaagc ccgaaaaatg tgctgttaat
      901 cacatgccta agtaaaaatt tgacgacacg tattgaagtg cttcaccata gcctacagat
      961 tatttcggag cgcgaaaata tagggagtat gcggtggttg ctgaaaacca gcttgggcac
     1021 attgatcaaa taaagcagac caacgcgggc gcggtttatc gcctgattga tcagcttggt
     1081 ccagtctcgc gtatcgatct ttcccgtctg gcgcagctgg ctcctgccag tatcactaaa
     1141 attgtccgtg agatgctcga agcacacctg gtgcaagagc tggaaatcaa agaagcgggg
     1201 aaccgtggcc gtccggcggt ggggctggtg gttgaaactg aagcctggca ctatctttct
     1261 ctgcgcatta gtcgcgggga gattttcctt gctctgcgcg atctgagcag caaactggtg
     1321 gtggaagagt cgcaggaact ggcgttaaaa gatgacttgc cattgctgga tcgtatcatt
     1381 tcccatatcg atcagttttt tatccgccac cagaaaaaac ttgagcgtct aacttcgatt
     1441 gccataaccc tgccgggaat tattgatacg gaaaatggta ttgtacatcg catgccgttc
     1501 tacgaggatg taaaagagat gccgctcggc gaggcgctgg agcagcatac cggcgtaccg
     1561 gtttatattc agcatgatat cagcgcatgg acgatggcag aggccttgtt tggtgcctca
     1621 cgcggggcgc gcgatgtgat tcaggtggtt atcgatcaca acgtgggggc gggcgtcatt
     1681 accgatggtc atctgctaca cgcaggcagc agtagtctcg tggaaatagg ccacacacag
     1741 gtcgacccat atggtaaacg ctgttattgc gggaaccacg gctgcctcga aaccatcgcc
     1801 agtgtggaca gtattcttga gctggcacag ctgcgtctca atcaatccat gagctcgatg
     1861 ttacatgggc aaccgttaac cgtggactca ttgtgtcagg cggcattgcg cggcgatcta
     1921 ctggcaaaag acatcattac cggggtgggc gcgcatgtcg ggcgcattct tgccatcatg
     1981 gtgaatttat ttaacccaca aaaaatactg attggctcac cgttaagtaa agcggcagat
     2041 atcctcttcc ctgtcatctc ggacagcata cgtcagcagg cccttcctgc gtatagtcag
     2101 cacattagcg ttgagagtac tcagttttct aaccagggca cgatggcagg cgctgcgctg
     2161 gtaaaagacg cgatgtataa cggttctttg ttgattcgtc tgttgcaggg ttaacatttt
     2221 ttaactgttc taccaaaatt tgcgctatct caatttgggc caggaaagca taacttagac
     2281 tttcaaggtt aattattttc ctagtttata tttgtgaagc ataacggtgg agttagtgat
     2341 gctggagcgt ttctttatta ccggtacaga cacttctgta gggaaaacgg tggtttcccg
     2401 cgcattgcta caagcgttag cctcccaggg aaaaacagtt gcgggatata aacccgtagc
     2461 gaaggggagc aaagagacac ccgaagggct gcgtaataaa gatgccctgg tgttgcagag
     2521 tgtttcaacc atcgaactgc cttatgaagc agttaatcct atcgcgttaa gcgaagaaga
     2581 aagtagcgtg gcgcacagtt gcccaatcaa ttacaccctc atttcaaacg gcctggcaaa
     2641 cctgaccgat aaagtcgatc atgtcgtggt agaagggact ggcggctggc gcagtctgat
     2701 gaatgatttg cgtccactct ctgaatgggt ggtgcaggaa cagttgccgg tgttgatggt
     2761 tgtcggtatt caggaaggtt gcattaacca tgcactgcta acagctcagg cgattgccaa
     2821 cgacgggctg ccgctcattg gctgggtggc taaccgaatc aacccaggac tggcgcatta
     2881 tgcggaagtc attgatgtgc tgggtaaaaa acttccggca ccgctcattg gtgaactgcc
     2941 ttatctgccg cgcgccgaac agcgagaact ggggcaatac atccgcttag ctatgctgcg
     3001 cagtgtgctg gcggtagata gagtcacggt gtaacgtccg cgaaattacc gacgcaatga
     3061 cgcaggcaat caataaaccg gggagtagct gatactcccc ggtcatttca catatcatca
     3121 acgtcgacat aatcggcgcg tgcgtggttg ccgccaacag tgtcgccatc ccggtcaatc
     3181 cgagtaaaag cgtaatttct tcgccatcag ggaaccataa tcccaggcta cgaccataca
     3241 acatgccaat ggcaagaccg ataaatagcg tcggtgtaaa gacaccaccg ggtgcgccgg
     3301 aaccgctact cgccagcacg gcacacagtt tacaaaggaa gatcccggca atgatcatta
     3361 acagtggtgg ggcggttaaa aaggattgta cggtgctata gccgttgccc cacactgcag
     3421 gtgtgaacag ggaaagcaga cccacgatca acccgcctag tgccagttgc cagggcggcg
     3481 caagtttgag actcacaaat ccacgatgac aggcgttcat taacgttaac aacagtggtc
     3541 cgcacagacc tgccagcaca cctgtactga taatcagcgc atagtcacta gcctgaaccg
     3601 ttactgagag ttgtacgctg tagagtaacg cgtcgctatg attaataaga ttgctaacca
     3661 gcaacgccac gacggcggaa ataatcaccg ggccgagaga ggccaacatc atagtgccaa
     3721 acagcacttc ggctataaat aaactgccag caagcggggc gcgataggcc gcagccattc
     3781 ccgccgcggc cccacaggcg atccataatt tccattcctg gcgtggcgta aaacgttggg
     3841 caaaacagga ggcggcaagg gcagctaaaa gaatcatcgc accttcgcga ccaattgcac
     3901 tgccgctggt tactaccagc agcgaggcaa gcgatttaac caggcttgct gcgtaatcga
     3961 actgtccatc ggtttgcaac gcttccatgt aatcggtcgg cgcatgaggg cgttgttggg
     4021 taaatttctg ccagcccatc agcaacaaac ccgccgccag tccgccgagc gccggagtta
     4081 gcagccgtcg ccagggggaa aggtttgtcg ctgcattgac cagactgccg gagtcattat
     4141 tgaggaacaa ccactccagt agcagcatcg catgacgaaa cccggcaacg gcaaaggccg
     4201 cgagaatacc gacgactgtt gcgataagca gacggcggaa catagtgcgc agatccggat
     4261 agatgtgtag atgatgcatg agcagaaatg ggcaatgaaa cacgggcgac tattttgcgg
     4321 caaaagaggc caaccgcaaa agcagtgcgt gctttaagag ataaaaaaac gtgacattgt
     4381 aacttattat tgccgaatcg cgcgtaatcg tcaccatccg gcaatattac ggtgatccta
     4441 tcgaaagagt ggtttaaccg cgacaggaat caacagttgt gattgccatt gcgccagcgt
     4501 taagcgagcc agttcaccca gtgcacggta gaaagggtgt tctgcttttt cgataaaaac
     4561 atcaagaaaa cgtgttgacc acggaaaaag gtgccatgcc agcaattctt cgcattctgt
     4621 ctggcgacca ttctccgcca accacgcagc catcaacagc agtgaaccaa aatgatcttc
     4681 cggttcgttt tgcttcattt caaactgaat gcctttctcg cgcatccact gacgaagtgc
     4741 caatgttgaa tcgccaaaca gcacagattc gcgatccagc cagaccgatc cccacggcgg
     4801 agacggcagt gcccacgggc cgacaaacaa acgctgccag gcctgggcgt gagtctcttc
     4861 acactgtgtc tgaaaagccg tcaccagcgg cgctaatgac gcttctggta gaggccactg
     4921 agtttcccag ccgtcactgg tgagtaccgc aaccagaggt gcggcttccg cactctctgg
     4981 agcgtaataa aacagcgcgc ccaacacgcg cgccgcgaca gaaaaattat cttgctgtga
     5041 aaaatgggtc attcctaaca tcctgaaatg tgcgggttgc cccgcaccaa tttgttaacc
     5101 tgcaatagcc attcctactg tcatgtgcag gccataaaag agcacacgcc cgatcatctc
     5161 accaacaagt atgaggatta gccccagaac aagtccggca acgtgaggtt cacggcgacg
     5221 aatgagcgga caaagccagc atccaagacc tgcgcataac aataccacac gccagacctg
     5281 taaggaggcg taatctggta ccagtgcgct ggcttgttgc acggatgagt gaatagacgc
     5341 cagagacaaa ccttgcagca caatcacccc ggcacaagcg atcagcgcga gaacgctaat
     5401 gatggcaaat ggtgtggtat taaatgtaac gcgggctgcc cgcagaattg cggcagcaag
     5461 tattggaccg ctcaacaata ccgtcaggaa gaaagccagc gtagtgtaac cgttatgcca
     5521 ggttggcaca gtgtcgattt gatacacgca ggtcatcatc cagacgaaaa tgacgccgag
     5581 cgccatgctg acaagtaacc agagtttccc caacgcttgc ggcattttac cgatgaccgc
     5641 caccagccac catagtccgc caacggcaaa aaatatggaa ccggcagcaa tctcgttact
     5701 cagaccagaa gctccgattc gattcagcga gttaaacgcc cgcagtggcg atcccaggtg
     5761 catgacagag gccataaacc cgatgcccat caacagccag aggaaaaaca tgccacggac
     5821 aatacgctga cggtctgcgt catttttggc ggcgaaccag ccaattccgc taacaatcag
     5881 cgcgcctact acgcattgac ccaatacggt aaagataacc agtggccact catgccatcc
     5941 atttcccatt ttacacttcc tccggattag ccagataacc agtggtatcg ctggtcgggc
     6001 ggctgttggc gttaggtttg ataacgatat tgggttttgt gaaatgcgcg cgcggcagtg
     6061 gcgcgacggc agccagagtg ccgtgcttct ggcgcagctc ttcaattgga ccgaactcaa
     6121 gtgcacgcag cgggcaggat tcgacacata tgggttgttt cccctcggcg acgcgcgaat
     6181 aacaaccatc gcacttcgtc atgtgccctt tttcagcatt gtactgtggc gcgccgtacg
     6241 gacaagccat gtggcagtag cggcagccga tacaaacatc ttcatcaacc accacaaagc
     6301 catcttcacg cttatgcatt gcgccgctgg ggcagacttt tgtacatgcg gggtcatcgc
     6361 aatggttaca ggagatggag agataataag cgaacacgtt ctggtgccag acgccattat
     6421 cttcctgcca gtcgccgcca gcatattcat aaatacggcg gaaactgact tccgggccaa
     6481 gatctttaaa atctttgcac gccagttcgc aggttttaca gccagtacag cggctggaat
     6541 cgataaaaaa tccatattgt gtggtcacgg gctactcctt aaaccttttc gatctggaca
     6601 agattgctgt gcgacgggtt tccctttgcc agcggtgaag ggcggtgaga ggtcagaata
     6661 ttgatactgc cgccgtgatc gacccggtca ccaaacatat ccgctttaag ccaagcacct
     6721 tgcccgatgg cggtaacgcc aggcagaata cgcggagtca cttttgcggc aatcagcatt
     6781 tctccattat tgttaaatac ccgcacggta tcgccatgac ggataccgcg tgcctgagca
     6841 tcaatggggt tgatccacac ctcttgtggg caggcctgct gtaacacatc aatattgccg
     6901 tagctggagt gggtacgcgc tttgtaatgg aagcccgtta actgcagtgg ataggttttc
     6961 cgcaggggat cgtcccagcc atcaaaacct ggggtatacg caggaagggg atgaataact
     7021 tcatcttttt tcaattccca ggtatccgca atcttcgcca gtcgttcaga ataaatttcg
     7081 attttccccg aaggtgtttt caacgggttt gcctgtggat cttcacggaa tgcgcggaaa
     7141 gcgacgtagt gttcttccgg gcattttttc ttaaagatcc cggtcgtttt catctcctcg
     7201 tagtcgggca tctcagggtt acgttccttc gttttcgcat ggagatattt gatccattca
     7261 tgctgactgc gaccttcagt aaaggtttga taaacgtctg gtcctaagcg tttggcgact
     7321 tcactcagca tccagtagat gggtttgcgt tcaaattttg ctgaggttgc gggttgggcg
     7381 aggatcacat agcccatatt ccctgcagat tcatgagaga taaggtcttc ttgctctgtt
     7441 ggcatcaggt cgggcaacag gatatcgcaa tacttagccg aggccgtcat gaagtggtca
     7501 atgccaacaa tcatctcgca cttgctgtca tcctgaagca cctcatgggt gtgattgatg
     7561 tcgccatgtt gattgatcaa tgtgttactg gcgtagcacc ataaaaactt gatggggaca
     7621 tccagttttt cttttccacg aacaccatca cgg
//
LOCUS       DAZOYL010000193         7545 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZOYL010000193 DAZOYL010000000
VERSION     DAZOYL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.194"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            60..722
                     /locus_tag="ACHUOY_002954"
     CDS             60..722
                     /locus_tag="ACHUOY_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR0683679.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            818..1276
                     /gene="mgsA"
                     /locus_tag="ACHUOY_002955"
     CDS             818..1276
                     /gene="mgsA"
                     /locus_tag="ACHUOY_002955"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR0683680.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(1308..3362)
                     /gene="helD"
                     /locus_tag="ACHUOY_002956"
     CDS             complement(1308..3362)
                     /gene="helD"
                     /locus_tag="ACHUOY_002956"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR0683681.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISSQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            3485..3931
                     /locus_tag="ACHUOY_002957"
     CDS             3485..3931
                     /locus_tag="ACHUOY_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR0683682.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            3941..6103
                     /gene="yccS"
                     /locus_tag="ACHUOY_002958"
     CDS             3941..6103
                     /gene="yccS"
                     /locus_tag="ACHUOY_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR0683683.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(6066..6695)
                     /gene="sxy"
                     /locus_tag="ACHUOY_002959"
     CDS             complement(6066..6695)
                     /gene="sxy"
                     /locus_tag="ACHUOY_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR0683684.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            6914..7423
                     /gene="sulA"
                     /locus_tag="ACHUOY_002960"
     CDS             6914..7423
                     /gene="sulA"
                     /locus_tag="ACHUOY_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR0683685.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
BASE COUNT         1754 a         1980 c         1815 g         1996 t
ORIGIN      
        1 tgattaaaga aaaattttga cacattatta ttcgggcggt cttttgacag gagctaccta
       61 tgaaaaccgg catcgtgacc accttaattg cattatgttt gccggtatcc gtttttgcca
      121 ccacgctgcg gctttcaacc gatgtcgatc ttctggtact cgacggcaag aaggtttcca
      181 gctctctgct gcgcggtgcg gacagcattg aactggataa tggaccgcat cagttagtgt
      241 ttcgcgttga gaagacgatt catctttcca atagtgaaga acggctgtat atctccccac
      301 cgctggtggt cagttttaat acccagctca tcaaccaggt caattttcgc ctgcctcgcc
      361 tggagaatga gcgggaagct aaccattttg atgccgcgcc gcgccttgaa ttgttggatg
      421 gcgatgcgac gccgattccg gtaaagctgg atattctcgc cattacctca acggcaaaaa
      481 cgattgatta tgaggtcgag gttgaacgct ataacaaatc cgcgaaacgc gcttcactac
      541 cgcaatttgc cacgatgatg gcagatgaca gtacgctgct ttcgggtgtt tccgagctgg
      601 atgctattcc accgcagtct caggtgctca cagaacaacg gctgaagtat tggtttaaac
      661 tggctgaccc acaaacgcga aatactttcc tgcagtgggc ggaaaaacaa ccatcttcct
      721 gagatttttg tgcctgtgcg caggcttttt cggtctttat cttgcagcga taagtgctta
      781 cagtaatctg taggaaagtt aactacggat gtacattatg gaactgacga ctcgcacttt
      841 acctgcgcgg aaacatattg cgctggtggc acacgatcac tgcaaacaaa tgctgatgag
      901 ctgggtggaa cggcatcaac cgttactgga acaacacgta ctgtatgcaa caggcactac
      961 cggtaactta atttcccgcg cgaccggcat gaacgtcaac gcgatgttga gtggcccaat
     1021 ggggggtgac cagcaggttg gcgcattgat ctcagaaggg aaaattgatg tattgatttt
     1081 cttctgggat ccactaaatg ccgtgccgca cgatcctgac gtgaaagcct tgctgcgtct
     1141 ggcgacggta tggaacattc cggtcgccac caacgtggca acggcagact tcataatcca
     1201 gtcgccgcat ttcaacgacg cggtcgatat tctaatcccc gattatcagc gttatctcgc
     1261 ggaccgtctg aagtaatatt gcacaggtgg caaacgccac ctgtttctta cggttttctc
     1321 gccaccggca catccagatt tttcagtatt tccacaaagg gagagggatt ctctttgtta
     1381 aacagtgccc atacccgatg gcgtgcccgg gtcagcgcca cgtacattaa ccgccgttct
     1441 tcagcgtccg ggaaatcctc aaccggtggc agtagcgcct cttccataat cgactcccgc
     1501 gccgcagccg gaaaaccatc acttccctcc tgcaagccaa cgatgatgac gtaatccgcc
     1561 tgttgccctt tgctggcatg aatggtcata aagtcgattt gcaacttcgg ccagcgtgtt
     1621 gccgcttttt ccaggctggc aggcctcatg tgatggtaac gcgccaggat cagaatgcgc
     1681 tcttccggtt tggcataacc agagagctta tccagcaaag cgtcaagttg actctcatcc
     1741 aataacgtga cggctttttt gtctccattg gttaagctgt ttagcggctt tttcagctgg
     1801 cctgggttct gctgaataaa ccggtttgcc accttaccga tacgactgtt aaaacggtaa
     1861 gtcgtgtcta aatcacaacg ttcgccttca ccaaagtttt catggaaagc ggtggtgagc
     1921 gacatttgcg caccgctgaa tcggtaaatc gcctgccagt catcaccaac agcgaacaac
     1981 gtcgtctgac tgttttgctt gcgtaatgcc gctaacaacg ctgcccgctg cgacgagata
     2041 tcctgaaatt catcaaccag aatatgcttc cacgggctga taaagcgacc tttctccaga
     2101 atcacaatcg cctgatgaat aaggcccgaa aaatcgacag cgttttctgc cttcagcgca
     2161 cctttccagg cttttaataa cggggccatc aacttgatac gtttactgaa cagatcgcga
     2221 atctcttcgg gtgcactggc aatcatttct gcctgtgcac caccgtgcat ccgcatcaga
     2281 cttacccagc gatcgaggcg agaggcaagg cgacgctgta atttttcatc atcccagaag
     2341 ttaccttctg gcactgacca ctgcatttct tccgtcagcc attgccgcca gcccttcgcc
     2401 tgcgctttct tttcgctgca ttgcttgcgc cactcagcaa taaagagttc atgacgggca
     2461 gcggtatcat tttccagttt gctgactatc ggaacttttt tgctgccctg ctgaataata
     2521 tgcagcgcca gcgcatgaaa cgtgcgtgcg gtaatgtctt cggtatgtag ccgttcgcga
     2581 atccgctcgt ccatctcttc agcggctttg cgaccaaacg ccagcaataa aatttgctca
     2641 ggggacgctt caccacgcgc cagcaaccag cctgcacggg ccaccagcac cgacgttttt
     2701 ccgcttcctg cacctgccag cactaacaga gaatgctcgc cattaacgac tgcccgggcc
     2761 tgcgccggat tcagcggtga agactcgacc tggcggaaaa aatccgcata ctcggtaagc
     2821 atggcttcgg tatacgcctg gttatgctgc aaccgagcgc tttcaatatc tttcaaccag
     2881 gcctgacatt tacgccacgc ctcacggcag ttatcgaatt cttccagtcg gttaaccggc
     2941 aacggcaacg ccgacaaagc ctggcggatt tgttgctgaa cgccagaggt ttgctcacgc
     3001 gtcagccatt tattttctcc agtgcgcgtg gcaatcaaat ccagttgctg gcgtaaaaca
     3061 ccagacgcaa tttcgctcat ctcgccactc caccgccgcc agtgagcatc aagatgatgg
     3121 taaaaacgct gcgtctcgcc ccattcggtg ccgtgcagac gcaccacttt ttcgtccggc
     3181 agtacaaatt ccagctcgcc ccataccaga ccgcgcttac agtgaatcgc cagtaattga
     3241 ttgaaaggaa taaggtattc gtggcgatcg ccggagactt taatcccggc attgaggatc
     3301 accgcccgat cgtaagggtg ctgtgccaga cgttttccaa gcgttgtcgc tttcagttcc
     3361 atgactctgt agatccacac gaaattattt cttatcagtg taaccgtcag agaaaacgtg
     3421 ctccagccca aaaaaacgtt acaattgccg cccattactg aaaaccacag taaagcgagg
     3481 ttttatgcgt accgttttga acattctgaa ctttgtgctt ggcggatttg ccaccactct
     3541 gggctggctg ttggcgactc tggtcagtat tgtgctgatt tttaccttac cgctgacacg
     3601 atcctgctgg gagatcacta aactatctct ggtgccttat ggcaatgaag ctattcatgt
     3661 cgatgaactg aacccggctg gcaaaaatgt gctgctgaat actggcggta cggtattgaa
     3721 tattttctgg ctgattttct ttggctggtg gttatgcctg atgcacattg caacgggcat
     3781 cgcacaatgt atttcaatca ttggcattcc tgtcggcatt gcgaacttta aaattgccgc
     3841 tattgcacta tggccggttg gtcgtcgcgt ggtatcggta gaaacagcgc aagctgcgcg
     3901 tgaagccaat gcacgtcgtc gttttgaata atccactaat atggccttta tgctaagtcc
     3961 tttgctcaaa cgctatacct ggaacagcgc ctggctgtat tacgcgcgta tttttattgc
     4021 gctttgtgga accacagcgt ttccgtggtg gctgggtgat gtaaaactga cgattccgct
     4081 aacgctgggg atggtggcag cggcgctgac cgatctcgat gaccgactgg cgggacgttt
     4141 gcgtaacctc atcattacgc tgttctgctt ttttatcgcc tcggcctcag tagaattgct
     4201 gtttccctgg ccctggctat ttgcgattgg cttaacgctc tctaccagcg gcttcatttt
     4261 gctcggcggt ctgggtcaac gctatgcaac aattgccttc ggtgcattgc tgatcgccat
     4321 ttacactatg ttgggaacat cactgtatga gcactggtat cagcagccga tgtatctgct
     4381 ggccggtgcc gtctggtaca acgtcctgac acttattggt catctgctgt tcccggtccg
     4441 cccgctgcag gacaacctgg cgcgttgcta tgaacaactg gcgcgttatc ttgagctcaa
     4501 gtcgcgcatg tttgatcctg atattgaaga tgaaagccag gcaccgctgt acgatttggc
     4561 tctcgccaac ggtctgctga tggcgacatt gaatcagacg aaactctcgc tgctgacccg
     4621 cttacgtggc gatcgtggtc aacggggaac gcgtcgcacg ctgcattatt actttgtcgc
     4681 acaggatatt cacgagcgtg ccagctcttc tcatattcag tatcaaacat tgcgtgaaca
     4741 ttttcgccac agcgacgtgc tgttccgttt tcagcggctg atgtcgatgc agggccaggc
     4801 gtgccagcaa ctgtcacgct gtattttgtt gcgtcagcct tatcaacatg atccgcattt
     4861 tgagcgcgct tttacgcata ttgatgctgc gctggagcgg atgcgcgata acggcgcacc
     4921 cgccgattta ctcaaaacac tgggattttt gctgaacaat ttacgcgcca ttgatgccca
     4981 actggcaaca attgaatcag aacaggccca ggcactaccc cataataatg acgaaaatga
     5041 gctcgctgat gacagcccgc acgggttgag tgatatctgg ctgcgtctta gccgtcactt
     5101 cacgccggaa tccgccctct tccgtcatgc ggtaagaatg tcgctggtat tgtgcttcgg
     5161 ctacgccatc attcagataa ccggaatgca tcacgggtat tggatcttgc tgacaagttt
     5221 gtttgtctgc cagccaaact ataacgccac gcgccaccgc ctgaagttaa ggattattgg
     5281 tacgctggta ggtatcgcca ttggcattcc tgtgctgtgg tttgtgccat cactggaagg
     5341 gcagctggtg ctgctggtta ttaccggcgt gctctttttt gccttccgta acgtgcaata
     5401 cgctcatgca acgatgttca tcacactttt ggtgctactg tgttttaact tactgggtga
     5461 aggttttgaa gtagcgttac ctcgcgtaat cgatacgctg attggttgtg ccattgcgtg
     5521 ggcggcagtg agctacatct ggcctgactg gcagtttcgc aatctgccgc gcatgctcga
     5581 acgcgccaca gaggccaact gtcggtatct cgatgccata ctggagcaat accatcaggg
     5641 gcgtgataac cgtctggcgt atcgtattgc ccgccgcgat gcacacaacc gtgatgctga
     5701 gctggcgtcg gtggtatcaa atatgtccag cgagccgaac gttaccccgc aaattcgcga
     5761 agccgcgttt cggttgctgt gccttaacca tacgtttacc agctatatct cagccctcgg
     5821 tgctcaccgg gagcagttaa ctaatcctga aattctggcg tttcttgatg acgcagtttg
     5881 ctatgttgat gacgcgttac atcatcaacc tgctgatgaa gaacgcgtca atgaggcatt
     5941 agctagcctg aaacagcgga tgcagcaact tgaaccacgg gcagacagca aagaacctct
     6001 ggtcgtacaa caagttggat tattgattgc attactgcct gagattggtc gtctgcaacg
     6061 ccagattact caagttccgc aggaaactcc tgtttcggcg taagagagtc agcccattct
     6121 gcaagctcct ggcggcgtgc caccgggagc gcagcttcat gaatgccgat aatggcacct
     6181 tcaagcataa acagaatctt ttctgtcacc agactgtttt gctgccgcag tcgcaaccaa
     6241 cacatttttg ccccaagtat acgtaacgcc cgtacatcct taatccccac ctccccgaga
     6301 atcgcttcca gatgaaaaga catattgggc aaatctttca gtctttcccg ggtattgcgc
     6361 gtgcttttct ctttcagcgc tgcatcgaga gaatatttcg acagacgcac cagcttcagt
     6421 tgatttcgcc atagactttc atcaacccga tagtaattga gggtaacgga tcggccacac
     6481 tttttatatg tcagccagac aggcggatgt tttacacagt actgtgcact ttgctcacaa
     6541 gcccgaagat acaactcacc atcagaaacc atcgcaaaca ccgtgtcgtc aacggtcagg
     6601 ctgtaactgc caaacaatga tcggtattca attgtgccca acgttgccag gtattcttgt
     6661 gatttataga tccgcttata ggagaggctt ttcataaaat tccttttaaa atcataacat
     6721 aaaagaatga ttcacattaa cggatccgtt aactacgaaa ataggcaact tattcttaag
     6781 gggcaagatt aatttatgtt ttcccgtcac caacgacaaa atttgcgagg ctctttccga
     6841 aaatagggtt gatctttgtt gtcactggat gtactgtaca tccatacagt aactcatagg
     6901 ggctggattg attatgtaca cttcaggcta tgcacatcgt tcttcgtcgt tctcatccgc
     6961 agcaagtaaa attgcgcgtg tctctacgga aaacactaca gccgggctta tcagtgaagt
     7021 tgtctatcgc gaagatcagc ccatgatgac gcaacttcta ctgttgccat tgttacagca
     7081 actcggtcag caatcgcgct ggcaactctg gttaacaccg caacaaaaac tgagtcggga
     7141 atgggttcag gcatctgggc tacccttaac gaaagtaatg cagattagcc agctctcccc
     7201 ttgccacact gtggagtcaa tggttcgcgc tttacgcacg ggcaattaca gtgtggtgat
     7261 cggttggttg gcagatgatt tgactgaaga agagcatgct gaacttgttg atgcggcaaa
     7321 tgaaggtaac gctatggggt ttattatgcg tccggtaagc gcatcctctc acgccacgag
     7381 acaactttcc gggctaaaaa ttcactctaa tttgtatcat taagtaaatt taggattaat
     7441 cctggaactt tttttgtcgc ccagccaatg ctttcagtcg tgactaattt tccttgcgga
     7501 ggcttgtctg aagcggtttc cgcgattttc ttctgtaaat tgtcg
//
LOCUS       DAZOYL010000194         7522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZOYL010000194 DAZOYL010000000
VERSION     DAZOYL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.195"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            11..1300
                     /gene="hpaG"
                     /locus_tag="ACHUOY_002961"
     CDS             11..1300
                     /gene="hpaG"
                     /locus_tag="ACHUOY_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR0683686.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1297..2763
                     /gene="hpaE"
                     /locus_tag="ACHUOY_002962"
     CDS             1297..2763
                     /gene="hpaE"
                     /locus_tag="ACHUOY_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0683687.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2765..3616
                     /gene="hpaD"
                     /locus_tag="ACHUOY_002963"
     CDS             2765..3616
                     /gene="hpaD"
                     /locus_tag="ACHUOY_002963"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR0683688.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3626..4006
                     /locus_tag="ACHUOY_002964"
     CDS             3626..4006
                     /locus_tag="ACHUOY_002964"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR0683689.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4117..4920
                     /gene="hpaH"
                     /locus_tag="ACHUOY_002965"
     CDS             4117..4920
                     /gene="hpaH"
                     /locus_tag="ACHUOY_002965"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR0683690.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4931..5719
                     /gene="hpaI"
                     /locus_tag="ACHUOY_002966"
     CDS             4931..5719
                     /gene="hpaI"
                     /locus_tag="ACHUOY_002966"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR0683691.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5894..7270
                     /gene="hpaX"
                     /locus_tag="ACHUOY_002967"
     CDS             5894..7270
                     /gene="hpaX"
                     /locus_tag="ACHUOY_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR0683692.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            7280..>7522
                     /locus_tag="ACHUOY_002968"
     CDS             7280..>7522
                     /locus_tag="ACHUOY_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR0683693.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         1716 a         2117 c         2141 g         1548 t
ORIGIN      
        1 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
       61 tgcatggcag gaagcgttcc agcaatcccc ctacaaagcc ccgcctaaaa ctgcggtctg
      121 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
      181 tgaaaaggta ctgagcggtg cgactgttgc gctgattgtg ggaaaaacgg cgacgaaagt
      241 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
      301 gccggaagag agcttttacc gcccggcaat caaagcaaaa tgtcgtgatg gattctgccc
      361 cattggcgaa accgtggctc tcagcaatgt cgataatctg accatctata ccgagatcaa
      421 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cgcagttgct
      481 gagcgccctg agcgaatttg ccacgctgaa tccaggcgac gccattctgc tcggcacgcc
      541 acaggcgcgc gtggaaatac agccaggcga tcgcgttcgt gttctcgcag aaggtttccc
      601 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcacaaga gcttcccaac
      661 gcagccacac ccgcacggca cgctgtttgc cctcggcctg aactacgccg accacgccag
      721 cgaactggaa tttaagccac cggaagaacc gctggtgttc ctgaaagcgc caaataccct
      781 cactggcgat aaccagacct ccgtgcgtcc aaacaatatt gaatacatgc actatgaagc
      841 ggagctggtg gtagttattg gcaaacaggc gcgtaacgtc agcgaagccg atgccatgga
      901 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgtgactatc tggaaaacta
      961 ctaccgccct aacctgcggg taaaaagccg cgacggactg acgccgatgc tttcaaccat
     1021 cgtgccgaaa gaggcgatcc cggacccgca taatctgacc cttcgcacct tcgtcaacgg
     1081 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
     1141 ctacttaagt gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacacccaa
     1201 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
     1261 cctggtgaac cgaattgtga gtgaggatac agcgaaatga aaaaagtaaa tcattggatc
     1321 aacggcaaaa atgttgcagg taacgactac ttccagacca ccaatccggc aacgggtgaa
     1381 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
     1441 aaagaggcgt tcccgaaatg ggccaatctg ccgatgaaag agcgtgcgcg cctgatgcgc
     1501 cgtctgggcg atctgatcga ccagaacgtg ccagagatcg ccgcgatgga aaccgcggac
     1561 accggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc ccacaacttt
     1621 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
     1681 atgctcaact acacgctggt gcagccggtg ggcgtttgtg cgctggtatc gccgtggaac
     1741 gtaccgttta tgaccgccac atggaaggtc gcgccgtgtc tggcgctggg caataccgcg
     1801 gtactgaaaa tgtcggaact ctccccgctg accgctgaca gcctgggtga gctggcgctg
     1861 gaagccgata ttccggcagg cgtgctgaac gtggtacagg gctacggcgc aaccgcaggg
     1921 gatgcgctgg ttcgtcatca tgacgtacgt gccgtgtcgt tcaccggcgg tacggccacc
     1981 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggaact gggcggtaaa
     2041 tcgccggtgc tgatttttga agatgccgat attgaacgcg cgctggacgc cgccctgttc
     2101 accatcttct cgatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcaa
     2161 agcatctacc cggaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
     2221 gatccgaacg atccgaatac ccaggttggc gcgcttatta gccagcagca ctgggaaaaa
     2281 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgctggc gggcggcccg
     2341 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaaccgtg
     2401 ctggcagacg ttgataaccg tatgcgagtc gcccaggaag agattttcgg gccggtcgcc
     2461 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgtc tggcaaacga cgtggagtac
     2521 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
     2581 attgaagctg gcatggtgtt cgtcaacacc cagaacgtgc gtgacctgcg ccagccattt
     2641 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
     2701 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
     2761 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcga tgtatcactc
     2821 tgaactgcca gggaaaaacc acggttgccg ccagggcgcg atcgacgggc ataaagagat
     2881 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata cccactggct
     2941 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
     3001 cgagctgccg cattttatcc gcgatatgac ctacaactac gagggcaacc cggagttggg
     3061 gcagcttatt gccgatgaag ccttaaagct cggcgtgcgg gcaaaagcgc acaacattcc
     3121 cagcctgaaa ctggaatacg gcacgctggt gccgatgcgc tacatgaatg aagacaagca
     3181 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
     3241 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
     3301 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag
     3361 ctacacccgc gagttcgacc gccagatgga cgagcgcgtg gtgaagttgt ggcgcgaagg
     3421 ccagttcaaa gagttctgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
     3481 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
     3541 ggtggagttt atcaccgagc tattcccaag ctctggcacc ggtcaggtta acgctgtttt
     3601 cccgcttccc gcgtaaggac gttttatgcc gcactttatc gttaaatgca gtgacaacat
     3661 ccgcgaagaa gccgacctgc cggggttgtt cgccaaagtg aatccgacgc tggcagccac
     3721 gggtattttt ccgctggcgg gtattcgcag ccgcgtgcat tgggtcgata cctggcagat
     3781 ggccgacggg cagcgtgatt atgccttcgt gcatatgacg ttgaaaatcg gcgcaggtcg
     3841 cagcctggaa agccgccagc aggcgggtga aatgctgttt gaactgatta aaacgcactt
     3901 cgccgccctg atggagagcc gcctgctggc gttgtcgttt gagattgaag agctgcatcc
     3961 gacgctgaat tttaaacaaa acaacgtgca cgcattgttt aagtagcgcg cagattgccc
     4021 ggtggcgctg cgcttaccgg gcctacaaaa ccccaaaccg tacaccgtag gccggataag
     4081 gcgcagccgc atccggcaat gccacaggat atcgctatgt tcgataaaca cacccacacc
     4141 ctgatcgccc agcgtctgga tcaggcagaa aaacagcgcg aacagatccg cgcgatctcg
     4201 ctggattacc cggagatcac catcgaagac gcttacgcgg tgcagcgtga atgggttcgc
     4261 ctgaaaatcg ccgaaggtcg cacgctgaaa ggccacaaaa tcggcctgac ctcgaaagcg
     4321 atgcaggcaa gctcgcagat cagcgaaccg gattacggcg cgctgctgga tgacatgttc
     4381 ttccacgatg gcagcgatat cccgaccgat cgctttatcg tgccgcgcat tgaagtggag
     4441 ctggctttcg tgctggcaaa accgctgcgt gggccaaact gcacgctgtt cgacgtctac
     4501 aacgccacgg actacgtgat cccggcgctg gagctgatcg acgctcgctg ccacaacatc
     4561 gatccggaaa cccagcgccc gcgcaaagtg ttcgacacca tttctgataa cgccgccaac
     4621 gccggggtga tcctcggcgg tcgtccgatc aaaccagacg aactggatct gcgttggatc
     4681 tccgccctga tgtatcgcaa tggcgtgatt gaagaaaccg gcgtcgccgc tggcgtgctg
     4741 aatcatccgg caaacggcgt ggcctggctg gcgaacaaac tcgcaccgta tgacgtacaa
     4801 ctggaagccg ggcaaatcat tctcggtggt tcgttcaccc gcccggttcc ggcgcgtaag
     4861 ggcgacacct tccacgtcga ttacggcaac atgggttcca ttagctgccg ctttgtttaa
     4921 ggagagaacg atggaaaaca gctttaaagc ggcgctgaaa gcaggccgtc cgcagatcgg
     4981 attatggctg gggctgagta gcagctacag cgcagagtta ctggccggag caggattcga
     5041 ctggttgttg atcgacggtg agcacacgcc gaataacgtg caaaccgtgc tcacccagct
     5101 acaggcgatt gcgccctacc ccagccagcc ggtagtacgt ccgtcgtgga acgatccggt
     5161 gcaaatcaaa caactgctgg acgtcggcac acaaaccttg ctggtgccga tggtacaaaa
     5221 cgctgacgaa gcccgtgaag cggtacgcgc cacccgttat ccccccgccg gtattcgcgg
     5281 tgtgggcagt gcgctggccc gcgcctcgcg ctggaatcgt attcctgatt acctgcaaaa
     5341 agccaacgat caaatgtgcg tgctggtgca gatcgaaacg cgtgaggcaa tgaagaactt
     5401 accgcagatt ctggacgtgg aaggcgtcga cggcgtgttt atcggcccgg cggatctgag
     5461 cgccgatatg ggttatgccg gtaatccgca gcacccggaa gtacaggccg ccattgagca
     5521 ggcgatcgtg cagatccgcg aagcgggcaa agcgccgggg atcctgatcg ccaatgagca
     5581 actggcaaaa cgctatctgg aactgggcgc gctgtttgtc gccgtcggcg ttgacaccac
     5641 cctgctcgcc cgcgccgccg aagcgctggc agcacggttt ggtgcgcagg ccaccgccgt
     5701 gaagcccggc gtgtattaat gccggatgcg ctgcgcttat ccggcctaca ctcgcaccaa
     5761 accgtaggcc ggataaggcg tttacgccgc atccggcaaa aagccgtacc aaatcgcgga
     5821 ccggataagg cgttacgccg catccgccat aaaccttgtc gtaccctaca aaaatcccat
     5881 tagaggaaga aaaatgagcg acacctcacc tgccataccg gagagtatcg atccggcgaa
     5941 tcagcataaa gcgctgactg ccggacaaca ggcggttatt aagaagctat ttcgccgcct
     6001 gagcgtcttt ctgttcgtgc tgtttatctt ctcgttcctt gatcgcatca acatcggctt
     6061 tgccggactc acgatgggac gcgacctcgg tctgagcgcc accatgtttg gcctcgctac
     6121 caccctgttc tatgccgctt atgtcatctt cggcattccc agcaacatta tgctgagtat
     6181 tgtcggtgcg cggcgctgga tcgccaccat catggtgctc tggggcatcg cctctactgc
     6241 caccatgttt gccactggcc ccaccagctt gtacgtactg cgtatactgg ttggcattac
     6301 cgaagccggc tttctgcctg gtattctgct gtatttaacc ttctggtttc cggcctattt
     6361 ccgcgcccgt gccaacgcct tgtttatggt agcaatgccg gtaacgacag cgttgggctc
     6421 gatcgtttcc ggctacattt tgtcgctgga tggcgtcatg gcattaaaag gctggcagtg
     6481 gctgtttttg ctggaaggct tcccgtcggt attactcggc gtcatggtgt ggttctggct
     6541 tgatgactca ccggacaaag ctaagtggct gacgaaagaa gacaaaaaat gcctgcaaga
     6601 gatgatggat aacgatcgtc tgacgctggt tcagccagag ggagccatca gccaccacgc
     6661 catgcaacaa cgcagtatgt ggcgggagat cttcactccg gtggtgatga tgtataccct
     6721 ggcgtatttc tgcctgacca acacacttag tgcgatcagc atctggacac cgcagatcct
     6781 gcaaagtttt aatcagggca gcagtaatat caccatcggc atgctggccg ccgtaccgca
     6841 gatttgtacc attctcggga tggtctactg gagccgtcac tcagatcgcc gccaggaacg
     6901 aaggcatcac accgcccttc cttatttgtt cgctgccgca ggttggttac tggcttcggc
     6961 aactgatcac aacatgatcc agatgctggg gatcattatg gcttcgaccg gatcattcag
     7021 cgcaatggcg attttctgga caacaccgga tcagtccatc agcctgcggg cacgagcgat
     7081 cggtattgcg gtgatcaacg ccactggcaa cattggttca gcgttaagtc cgtttatgat
     7141 cggctggttg aaagatttga ccggcagctt taacagtgga ttgtggtttg ttgccgcgct
     7201 gctggtgatt ggtgcgggga ttatctgggc gattccaatg cagtcctccc gtccgcgagc
     7261 gaccctgtaa ggaacgacga tgtgtgaccg tcagattgcc aatattgata tcagcaaaga
     7321 gtacgatgaa agcctgggca cggacgatgt gcattatcag tcgttcgccc gcatggcggc
     7381 attttttggc cgccatatgc tgccacatcg ccacgaacag tactttcaga tgcatttcct
     7441 caatagcgga cagattgagc tacagcttga cgatcatcgc tactcggtgg aagcgcccct
     7501 gtttgtcctg acgccgccgt ca
//
LOCUS       DAZOYL010000195         7518 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZOYL010000195 DAZOYL010000000
VERSION     DAZOYL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.196"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..675)
                     /locus_tag="ACHUOY_002969"
     CDS             complement(<1..675)
                     /locus_tag="ACHUOY_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR0683694.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAV"
     gene            complement(686..1687)
                     /gene="ltaE"
                     /locus_tag="ACHUOY_002970"
     CDS             complement(686..1687)
                     /gene="ltaE"
                     /locus_tag="ACHUOY_002970"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR0683695.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1724..3442)
                     /gene="poxB"
                     /locus_tag="ACHUOY_002971"
     CDS             complement(1724..3442)
                     /gene="poxB"
                     /locus_tag="ACHUOY_002971"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR0683696.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3575..4543)
                     /gene="hcr"
                     /locus_tag="ACHUOY_002972"
     CDS             complement(3575..4543)
                     /gene="hcr"
                     /locus_tag="ACHUOY_002972"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR0683697.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4555..6207)
                     /gene="hcp"
                     /locus_tag="ACHUOY_002973"
                     /gene_synonym="priS"
     CDS             complement(4555..6207)
                     /gene="hcp"
                     /locus_tag="ACHUOY_002973"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR0683698.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6351..7250)
                     /gene="lysO"
                     /locus_tag="ACHUOY_002974"
     CDS             complement(6351..7250)
                     /gene="lysO"
                     /locus_tag="ACHUOY_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR0683699.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1813 a         2038 c         1980 g         1687 t
ORIGIN      
        1 caccgccata aaatgttcaa actgttgctg ataactgagc acctctggtc cggcggcttc
       61 gaagatgcgg tgttcgctgg ccggatgatc taacaacgcc accagatagt gcagcaagtt
      121 ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta acactggcag
      181 gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcgccaa cgataattcc
      241 ggcccgaagt tcggtcacag gtacattcgc ttcacgaaga atgtccgccg tagcctgacg
      301 agcacgcaga tgatccgact gctcatgtgg cggggcctgc aacgaactga gaaagattaa
      361 ttgcttaact ggtacttcac gtagcgcatc gcggacgttg agagccacct ggcgctcctg
      421 agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga aaatagacgg tatcgatatc
      481 ctgcaacagg gccggaaggt tatccggcca gctgagatcg actttatggc aactgacgtt
      541 tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgccagga tctgatgccc
      601 ttgctggctg agtgtgcgca ccagatgctg accaatgtag ccactggcac cgagaactaa
      661 aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa tgcgcgccag tgggcggcga
      721 cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac caggcggaca atcggcgagg
      781 cgttaatcag cacgtttctc gctttcatgt attcgcctaa cgcggcagca ttttcttccc
      841 cgacgcgaac aaacagcata ttagtgtcct gacgcatcac atccgcgcct gcttcacgca
      901 gttgctccgc catccaggcg gcgttgtcgt gatcttcctg caaccgtgcg acgttatttt
      961 tcagggcata catcccggcg gcagccagaa tcccggactg acgcatcccg ccaccggcca
     1021 ttttccgcca gcggatggca cgtttaatgt aatcacgatt accgacgagt aatgaaccga
     1081 ctggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat tgcgtgatct
     1141 ctttcagttc gcagccgtaa gccaccacgg cattaaagat gcgcgcaccg tcaacatgca
     1201 gcgccagatt gcgcttgcgg gtaaattccc atgcttcttt caggtattca cgcggcagca
     1261 ctttgccgtt gtgggtgttt tctagactga gtaatttggt gcgggcgaaa tggatatcgt
     1321 cgggtttgat tttcatcgcc actttatcca gcggtagcgt gccgtcggca gccgcgtcga
     1381 tgggttgcgg ttgaatactg cccagcaccg ctgcgccacc ggcttcaaac agataattat
     1441 gcgcggcctg accgacaata tactcttcgc cgcgttcgca gtgactgagc agagcgacca
     1501 ggttagcctg ggtgccggtc ggcagaaaaa tggcggcttc tttaccggaa agctctgcgg
     1561 cgtagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc ccaaccgggg
     1621 cggccatcat tgcttcgagc atggcgcggc tcggtcgggt aacggtatca ctgcgtaaat
     1681 caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt agccagttcg
     1741 ttttcgccag ttcgatcact tcatcgccgc gtccgctgat gattgcgcgc aacatataca
     1801 agctaaatcc tttggcctgt tcgagtttga tctgcggtgg aatggctaac tcttctttgg
     1861 cgaccaccac atccaccagc accggaccgt cgatggagaa ggcgcgttgc agggcttcat
     1921 caacttcaga cgctttttct acacggatac ccgtaatgcc gcacgcttcg gcaatgcggg
     1981 caaagtttgt gtcgtgtagt tcggtgccgt cagtcaaata gccaccagct ttcatctcca
     2041 tcgccacaaa gcccagcacg ctgttgttaa agacgacaat tttcactggc agtttcatct
     2101 gcactactga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg cacatggcga
     2161 ccacctgacg ttctggctct gtcgcctgcg cacccagcgc ctgcggcatg gcgttagcca
     2221 tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttt agataacgtg
     2281 ccgcccacac cgttggcgta ccaacgtcac aggtgaaaat agcgtcatcg gcggcaaaat
     2341 gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt ttagctaaat
     2401 cgtccagccc tttgcgggcg tcgcggtaat cttccagcgc tttatccaga aacttgcgat
     2461 cggctttttc ttccaccaat ggaagcaatg cacgcagagt cgacttgata tcgccgacca
     2521 gtgccatatc caccttgctg tgagcgccga tgctggctgg gttgatatca atctgaatga
     2581 ttttggcatc ggtcgggtag aaggcgcggt agggaaattg cgtgccgagt agcactaacg
     2641 tgtcggcgtt catcatggta tggaaacctg acgagaagcc gattaacccg gtcattccaa
     2701 catcatacgg attatcgtat tcgacatgtt ctttaccgcg cagggcatga acaataggcg
     2761 ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcgcag ccgctgccac
     2821 acatcagggc gatattgctg gaataacgca gcagttgcgc cagtttgcgt aactcttctt
     2881 cttccggcgt cacgactggt tgtggcgcat gataccagtg cgtggttgcc ccttctggcg
     2941 caggttttaa cgccacgtcg cctggtaaca cgacaaccga aacgccacgg ttaagcaccg
     3001 ctttgcgcat ggcaatcgcc agtacttgtg ggatctgctc cgggctggaa accagctcgc
     3061 aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa tagccgctgc
     3121 caatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtga ttgcggtggc
     3181 aatcgaacag gccgttgatt aagtgcaggt tgccggggcc gcacgatccg gcgcagaccg
     3241 ccagttctcc gctaagttgt gcttcagcgc cagcggcaaa ggccgccact tcttcgtggc
     3301 gggtggacat ccactcgatg gtgcccatgc gattaagact gtcactaaga ccgttcagag
     3361 agtcgcctgt gactccccag atgcgtttca cccctgccga ttcgagtgtt ttggcgatat
     3421 aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg gtaacaagtt
     3481 tagttcatct gacggagggg gaagggatgg gagagaaagg aggcactaac ggttaaatag
     3541 cccgatgaaa ggaatatcat cgggcataag gcgattatgc gagaaccaaa tccccctgcg
     3601 gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc agcgtcattg
     3661 tactgctcac cgtatattca ccggaaacca cttttgtctt acagcagccg caaacacccg
     3721 cacggcaggc agcgacaacc ggaacgttat tgctttccag cgcctccagt agcgtggtgc
     3781 caaccggggc gtaaaattct cgtgccggtt gcagtttggt gaatttcaga ccgctggtcg
     3841 ccgcttctgc tactggagtg aagaatttct ctttaaagaa acgcgtcacg ccgagcgctt
     3901 tcacttcctg ctctacccaa tccatatacg gagccgggcc gcaggtcatc acggtacgtg
     3961 aagctaagtc aggtacacct gccagcagtt cgcgagtgag acgaccagcg ataaagcctt
     4021 cggtaacgtt attttctgcc accagcgtta ccggatagtt acgccactca tcggcgaaaa
     4081 taacatcctg cggcgtacgc acgttgtaga tcacccgcac atcggcctgt ggacggttct
     4141 tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc gccagcaaca
     4201 ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc gaaagccaga
     4261 gataatcacc gcgttttaca tcgcgcgtca gccactggga gccgacaccg tcatcaatcc
     4321 gccgcacggt cagggtgata tattcactca cgcctggcgt ggaggaaatg gtgtaagcac
     4381 gcagcgtttc cgctgagttt cgcacgctga ccagtgcata ttgcccggcg cgatatgggt
     4441 agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc gtaatgtgat
     4501 gaacctgcat ccgccacggg cattgattcg ttggcatcgt catcgacaaa ctccttacgc
     4561 gctcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc cgaatttctc
     4621 gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc cggtgacgat
     4681 atttttcacg cccagagaaa gcagcgtcag cagaatgacg atcgctttct gttcaaacca
     4741 ggagagcacc agcgacagcg gcagatcgtt cacaccacag cccagtttct ctgccagagt
     4801 gacagccaga ataatcgctg agtaagcatc gttacattga cctgcatcta ccagacgcgg
     4861 cagaccttcg atatcgccaa actcaagttt gttaaagcga tatttaccac aggcgagggt
     4921 caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt ggcgctcgcc
     4981 gcgtgcgccg tcacagccac caagcaggaa gatatgacgc agtttttcac ggctcaccag
     5041 atcaatcagc gtatcagcag cgccaagcag cgtctggcga ccaaaaccca cggtgataag
     5101 gtgcggaatt tcgctgtacg ggaagcctgc catctgttgc gcctgggtga taaccgcaga
     5161 gaaatcatca ccgtccagat gacgcacgcc aggccagcca acaatgctgc gggtccagat
     5221 acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca tcacgatggg
     5281 gccagggaaa cgagcgaact ccacttgctg attctgccag ccgctgccgt agttaccgac
     5341 cagatgcttg aatttacgca gctccgggta gccatgcgca ggcagcattt cgccgtgggt
     5401 gtagacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat ctttgagatc
     5461 gtgaccggaa atcagaatgc atttacccgc cgtcgctttg acgttgacct gggttggcgt
     5521 cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt tgaagttcat
     5581 ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc aggtccccag
     5641 ccacgccatg attttatggt actgggcata aatatcgttg tcgtattgac cgagaacatg
     5701 cgcgtgttcc atataggccg ccgcaccttt caggccatac aggcacagca gacgcaggcc
     5761 gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg ctgcctgacg
     5821 ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca gccatcgggt tatcgacgcg
     5881 cgcgttggca tctacagcca ggcattgtgc tttcagcgcc tcgcgcaagg caatcgcttc
     5941 acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg ttgagaaaaa
     6001 ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg ccgtattcac acgcttttac
     6061 cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt cagaagtttc
     6121 cgccgtttta ccacacatcc cctgcgcgta tgagcagccg tttcctgccg gagtacggat
     6181 agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat ttaatataca
     6241 tgtttaaggt taagacgctt aacgcgggga taaaagggat ttttcatgca actttaaggg
     6301 agattgattt agcgcaattt tggcggcagg gatctaccgc cagagaggta ttacgcagag
     6361 aaaaaggcga tgaggatcgg cactaacagg ctaagaataa aaccgtgaac aattgccgcc
     6421 gggaccatat ccagcccgcc agtacgttga agaacgggca gggtgaaatc cattgatgtg
     6481 gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa tcagcccagg gatcaacata
     6541 atagcaatca gttcacgggc cagatcatta aaaaacgccg cgctcccgat taccggacca
     6601 aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccgga ggccattgcc
     6661 agcgcggtat tgatggggag atcaagaata aaggcgttaa ttaaaccacc aattaatgaa
     6721 ctgacaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat ctgctttaag
     6781 gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa gtagcaaaat taacgtgtat
     6841 tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac tcagaccaat ggcaaaacca
     6901 atcactacta cgccgcacag ttttagcgac tccagcgcca tcgcaatacg cgacgggagt
     6961 ttttcttgct gatggtggtt gcgccacggc aggcctcgct ccagccacat cagggcggca
     7021 atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat cgccaacagg
     7081 ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag aataaggtaa
     7141 accatccagc ttaatagctg attaataact tttaacgcag cttgttggcg aagcggaatg
     7201 aggtaaccta caatcagggg aaccagaatg attaacagcc cagaaaacat gaaaacccag
     7261 tccttgcaaa gatgaagtcg aaatgcgcga tgacacacta ctgaaagcgg aaggacgagt
     7321 aaagttgcaa ttaaaaggaa atgttatgca taaggagcag tagagtattc gttttcattt
     7381 aaagatattc ttgcgcttta attacaaact gcaccgatgt tggtggcgtc aaaatcgccg
     7441 aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg cgttttacag
     7501 ggatgttacc tcgcgccc
//
LOCUS       DAZOYL010000196         7479 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZOYL010000196 DAZOYL010000000
VERSION     DAZOYL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.197"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(41..781)
                     /gene="cysQ"
                     /locus_tag="ACHUOY_002975"
     CDS             complement(41..781)
                     /gene="cysQ"
                     /locus_tag="ACHUOY_002975"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR0683700.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            971..2914
                     /gene="cpdB"
                     /locus_tag="ACHUOY_002976"
     CDS             971..2914
                     /gene="cpdB"
                     /locus_tag="ACHUOY_002976"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR0683701.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDAPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3032..3412)
                     /locus_tag="ACHUOY_002977"
     CDS             complement(3032..3412)
                     /locus_tag="ACHUOY_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR0683702.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3501..4361
                     /gene="qorB"
                     /locus_tag="ACHUOY_002978"
     CDS             3501..4361
                     /gene="qorB"
                     /locus_tag="ACHUOY_002978"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0683703.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4469..5434
                     /locus_tag="ACHUOY_002979"
     CDS             4469..5434
                     /locus_tag="ACHUOY_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0683704.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5542..6204
                     /gene="ytfE"
                     /locus_tag="ACHUOY_002980"
     CDS             5542..6204
                     /gene="ytfE"
                     /locus_tag="ACHUOY_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR0683705.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6249..>7479)
                     /gene="cycA"
                     /locus_tag="ACHUOY_002981"
                     /gene_synonym="dagA"
     CDS             complement(6249..>7479)
                     /gene="cycA"
                     /locus_tag="ACHUOY_002981"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR0683706.1"
                     /translation="IIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYFTGW
                     TYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEMEFW
                     FAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGFQIA
                     VFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSVVPE
                     KSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKAFAK
                     LSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILCSYL
                     VYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWFIAL
                     GLGWLFIGKKRAAELRK"
BASE COUNT         1903 a         1969 c         1906 g         1701 t
ORIGIN      
        1 atggcagaat caggaaatac actgtttctg ccatctgaat ttagtaaata gacactctga
       61 accccggatt caggaacgac tcgcgcggag tgtaatccag cggtttaccc tgccagtcgt
      121 gaacgtgcgc tccggcagct gcagccacag catgtccagc ggcggtgtcc caaatattcg
      181 ttggtccgaa gcgcgggtac agctgcgcct gtccttccgc caccaggcag aatttcagcg
      241 aagagccgat ggacgtggtc tgatgttcgc caagctgttg cagatactct ttcagctccg
      301 catctgcatg ggaacggctg ataaccacca gcggcgggcg cgcatcgcgg acctgaatct
      361 gcttgcgcac accgcactct tctttccagg ccttgccttc tgccgcgctg tacattacgt
      421 tcattaccgg cgcatacacc acacctaaaa tcggtttgcc atggtcaatg agcgcaatgt
      481 taacggtgaa ttcgccatta cgtttaataa actctttagt accatccagc gggtctacca
      541 gccagtaacg ctgccagtgc tgacggactt cccaaccggg aggatcttct tcagaaagga
      601 ccggaatatc cggtgtcagc gtacgtaaac cgtccatgat aacggtgtgt gcggcaatat
      661 ccgctgccgt taccggagaa ttgtccgctt tgctgacgac gtccatcggt ttcgtcccgt
      721 cgtagacctg cataatggca tcgcctgcat tccgtgcaag ctggcatact tgatctaaca
      781 tttctccacc tcgtctctgt gagcggtgtt aacttattgt tttacttata ccctatcgtt
      841 aatgaatgcg ccaactgtga tagtgtcatc attttcaaag cgtaaaattg tggcattctt
      901 cactgttcta taagtaagac gtttattctc ccttttcttt cgtattcccg atgataaaag
      961 gatgtccctg atgattaagt ttagcgcaac gctcctggcc acgctgattg ccgccagtgt
     1021 gaatgcagcg acggtcgatc tgcgtatcat ggaaaccact gatctgcata gcaacatgat
     1081 ggatttcgat tattacaaag acaccgccac ggaaaaattc ggactggtac gtacggcaag
     1141 cctgattaac gatgcccgca atgaagtgaa aaacagcgta ctggtcgata acggcgattt
     1201 gattcagggg agtccgctgg cagattacat gtcggcgaaa ggattaaaaa caggtgatgt
     1261 tcatccggtt tataaggcgc tgaatacgct ggattatacg gtcggtacac tcggcaatca
     1321 tgaatttaac tacggtctgg attacctgaa aaatgcgctg gcgggagcga aattccctta
     1381 tgtaaatgcc aacgtcattg acgccagaac caaacagcca atgtttacac cgtatttaat
     1441 taaagatacc gaagtggtcg ataaagacgg aaaaaaacag acgctgaaga ttggctatat
     1501 tggcgtcgtg ccgccgcaaa tcatgggctg ggataaagct aatttatccg ggaaagtgac
     1561 ggtgaatgat attaccgaaa ccgtgcgcaa atacgtgcct gaaatgcgcg agaaaggtgc
     1621 cgatgtcgtt gtcgttctgg cacattccgg gctgtctgcc gatccgtata aagtgatggc
     1681 ggaaaactca gtttattacc tcagtgaaat tccgggcgtt aacgccatta tgtttggtca
     1741 tgctcacgcc gttttcccgg gtaaagattt tgctgatatc gaaggggctg atatcaccaa
     1801 aggcacgctg aatggtgttc cggcggtaat gccgggcatg tggggcgatc atcttggggt
     1861 ggtcgactta caactcagta atgacagcgg taaatggcag gtgacgcagg cgaaagcgga
     1921 agctcggccg atttacgaca tcgccaataa aaaatccctc gcggcggaag acagcaagct
     1981 ggtagaaaca ctcaaagccg atcacgatgc cacacgccag ttcgtcagca agccaatcgg
     2041 taaatctgcc gacaatatgt atagctatct ggcgctggtg caggacgctc cgaccgtgca
     2101 agtcgtgaac aacgcgcaaa aagcgtatgt cgaacattac attcagggcg atccggatct
     2161 ggcaaaactg ccggtgcttt cagctgccgc accgtttaaa gttggtggtc gcaaaaatga
     2221 cccggcaagc tatgtggagg tggaaaaagg ccagttgacc ttccgtaatg ccgccgatct
     2281 ttatctctac cccaatacgc tgattgtagt gaaagccagc ggtaaagagg tgaaagagtg
     2341 gctggagtgc tccgccggac agtttaacca gattgatcct aacagcacga aaccacagtc
     2401 actcatcaac tgggatggtt tccgcactta taactttgat gttattgatg gtgtgaatta
     2461 tcagattgat gttacccagc ccgcccgtta tgacggcgag tgccagatga ttaataccaa
     2521 tgcggaaagg attaagaacc tgacctttaa tggcaagccg attgatccga acgccatgtt
     2581 cctcgttgcc accaataact atcgcgctta cggcggcaaa tttgccggta cgggcgacag
     2641 ccatatcgct tttgcttcac cggatgagaa ccgctcggtg ctggcagcgt ggattgctga
     2701 tgagtcgaaa cgtgcggggg aaattcaccc ggcggcagat aacaactggc gtttagcacc
     2761 gatagctgcc gataagaaac tggatatccg tttcgaaact tccccgtcag ataaagccgc
     2821 agcgtttatt aaagagaaag ggcagtatcc gatgaataaa gtcgcgaccg atgatatcgg
     2881 gtttgcgatt tatcaggtgg atttgagtaa gtaaaacact tctttttcgg cctataaatc
     2941 atcaatcgca tccggcattt attggcggat gcgatgctgg cgcatcttat ccgccctaca
     3001 agtcatacac cgtaggccgc gcatccggca gttatgccgc acgttcatcc cgcaccgcca
     3061 acacctcggg caaattcaac tcaatccagt ccgccagtgc agcaaccttt tcgctcacct
     3121 gctcgcccag cggcgtgagg ctgtattcaa catgcggtgg caccaccgga tacgcgatac
     3181 ggttaagaaa accatcctgt tctaacgcct gtaacgactg cgcaagcatc ttttcgctca
     3241 ccccgccgat tttgcgccgc aggtcgctaa agcgatgagt accttcgcgt agcgccacca
     3301 gaatcaacac cccccaacgg ctggtgacgt gtttcaacac ctcgcgcgac gggcactgtt
     3361 ccgcaaagag attaccctct ttcagttgtt gcgacaggct aacctgactc atttcatact
     3421 tacctttttg tacgtactta ctaaaagtaa gtttaggtgt tagcgtattt aaacacaaga
     3481 caaaacgatg gagacttccc atgatcgcta ttactggtgc cactggccaa cttggtcact
     3541 atattattga atccttgatg aaaacggttc ctgccagcca aatagtggct atcgttcgta
     3601 atccggcaaa agcccaggct ctagcagcac aaggcattac cgtgcgtcag gctgactacg
     3661 gcgatgaagc cgcactgaca tctgcacttc agggagtgga aaaactactg ctgatctctt
     3721 ccagcgaagt gggtcaacgt gccccgcagc atcgtaatgt tattaatgcc gcaaaggcgg
     3781 ctggcgtgaa atttatcgct tataccagcc tgctacatgc agatacctcc ccgctcggcc
     3841 tcgccgatga gcacattgag acggagaaaa tgctggctga ttctggcatc gtttacaccc
     3901 tgctgcgcaa cggctggtac agcgaaaact acctcgccag cgccccggca gcactggaac
     3961 acggcgtatt tatcggtgcg gcgggcgatg gcaaaatcgc ctcagcaacg cgggcagatt
     4021 atgcggcagc tgcggcacgc gtgattagcg aagccggtca cgaaggcaag gtttacgaac
     4081 tggcgggcga tagtgcctgg acgttgacac agttagcggc agagctgacc aaacagagcg
     4141 gcaaacaggt tacctatcaa aatctgagcg aagccgattt cgccgcggca ctgaaaagcg
     4201 tcggactgcc cgacggactg gcggatatgc tggcggattc tgacgttggc gcatcgaaag
     4261 gtggtctgtt tgataacagc aaaacgctta gcaaattgat tggccgccca acgacaacgt
     4321 tagccgaaag cgtaagccat ctttttaatg ttaataacta gttaattaaa gtggcatcct
     4381 cccgcatcct ctctgataat gacgggatgc cgggagcaat catgtctgct tcctgaactt
     4441 tcttctgaca gaccaatgga tgccagtaat gattagcggc gtgctgtacg ccctgttagc
     4501 agggttgatg tgggggctta tttttgtcgg gccgttgatc gtaccggaat acccggcgat
     4561 gttgcagtcg atggggcgtt atctggcgtt agggttaatt gcgctgccca ttgcctggct
     4621 gggacgcgtg cgtctgcgtc agttggcgcg tcgcgactgg cttaccgcct tgatgctcac
     4681 catgatgggc aaccttatct attacttctg ccttgccagt gccattcaac gtactggcgc
     4741 gcctgtttcc acgatgatta tcggcaccct gccggtggtg attcccgttt ttgccaatct
     4801 gctttatagc cagcgcgacg gcaaactcgc atggggaaaa ctcgccccgg cactggtttg
     4861 tattggcatc ggcctggcgt gtgtgaatat tgctgagtta aaccacggac tccccgattt
     4921 tgactgggca cgttatacct ctggcatcgt gctggcgtta gtttccgtgg tctgctgggc
     4981 gtggtatgcc ctgcgcaacg cccgctggct gcgggaaaat cccgacaaac atccgatgat
     5041 gtgggcaacg gcgcaggcgc tggtcacact gccggtttct ctcatcggct atctcgtcgc
     5101 ctgttactgg ctgaatatac aaacgccgga cttctcctta ccttttggcc cccgtccgct
     5161 ggtgtttatt agtctgatgg ttgcgatagc cgtgctttgc tcatgggttg gcgcactctg
     5221 ctggaacgtc gccagccagc gattaccgac agtgattctc gggccgctga ttgttttcga
     5281 aacgctggca ggtttgctgt acaccttttt actccgccag caaatgccgc cgctaatgac
     5341 gctgagcggt atcgcgctgt tagtgattgg cgtggtcatt gcggtcagag caaaaccaga
     5401 aaaaccttta actgaatctg tctcagaaag ttgacacgct ggcagtgagt taaataagcc
     5461 tctgctacgt aagggttata gcttttgcct taaagatgca tttaaaatac atcttatatt
     5521 attaagaatg aggtatcagc tatggcttat cgcgaccaac ctttaggtga actggcgctc
     5581 tctattcctc gcgcttcagc tctgtttcgt aaatatgata tggattactg ctgtggcggt
     5641 aagcaaacgc tggcgcgcgc ggcggcacgt aaagaactgg atgttgaggt cattgaagct
     5701 gaactggcaa agcttgctga acaaccgatt gagaaagact ggcgtagcgc cccgctggca
     5761 gaaatcattg accatatcat cgtgcgctac cacgatcgtc accgcgagca actgccggag
     5821 ctgatcctgc aagcgactaa agtcgagcgc gttcacgccg acaaaccgag cgtgccaaaa
     5881 gggctgacaa aatacctgac tatgctgcat gaagagctat ccagccacat gatgaaagaa
     5941 gagcagatcc tcttcccgat gatcaaacaa ggcatgggca gccaggcaat gggaccaatc
     6001 agcgtgatgg aaagcgagca cgatgaagcg ggcgaactgc tggaagtgat taaacacacc
     6061 accaataacg tcacaccgcc gccggaagcc tgcaccacct ggaaagcgat gtataacggt
     6121 attaatgaac tgattgatga cctgatggat cacatcagcc tggaaaacaa tgtactgttc
     6181 ccacgcgcgc tggcgggtga gtaatctaaa agctggatgg cattgcgcca tccagcatga
     6241 taatgcggtt atttccgcag ttcagcagcc cgcttcttac caataaacaa ccagcccagc
     6301 cccagtgcga taaaccacag cggggtgacc aacagcgcct ggcgagtgtc atcttccagt
     6361 gtcagcaaca cgaccacgaa cacaaagaac gccatacata cccagcacat cagcttgccg
     6421 agcggcatct tgtagatcga cttctcatgc agatgaggac gctgtttgcg gtaaaccagg
     6481 tacgagcaaa ggataattgt ccagacgaac ataaacagaa tcgcggaaac ggttgtaatc
     6541 atcgtgaacg cgccaatcac gctaggattc acatacaaca tcaccacgcc gccgagcaga
     6601 cagatacacg agaaggtcaa ccctttcgcg ggtactgcgc gcttagaaag tttagcgaac
     6661 gctttcggtg ccacaccttc ctgcgccaga ccaaacagca tacggctggt agagaagacg
     6721 ccgctgttag cggaagacgc cgcagaggtc agcaccacaa agttgatcac gctggcagcc
     6781 gcaggcaaac ctaccaacac aaacagttca acaaacgggc tcttctccgg gactaccgaa
     6841 ctccacggcg tcacggacat aatcacaatc agcgcgaaga cgtagaacat aatgatacgg
     6901 atcggaatgg agttaatcgc gcgtggcagt gatttctccg gatctttggt ttccgcagcg
     6961 gttgtaccta ccagctcaat ccctacgaaa gcgaaaaccg ctatctggaa tccggcaaag
     7021 aagccactta aacctttcgg gaaccagccg ccatcattcc acaaatgcgc gaatgacgct
     7081 tccacaccag tcggtgactg aaagtgcatc gccaccataa ccaggccgac gacaatcagc
     7141 gacacaatgg cgacgatttt gatcatcgca aaccagaact ccatctcacc gaacattttc
     7201 acggtggcga gattgagcgt cagcagcagc actatcaccg ccagcgaggc gacccagtcg
     7261 gagagatcgg ggaaccagaa ctgagcataa gcagtgatcg ccaccacgtc tgccataccg
     7321 gttacaaccc agcagaacca gtaagtccag ccggtgaaat atcctgccca cggcccgagt
     7381 aaatcggaag cgaagtcact aaaagatttg tattccagat tcgaaagcag caattccccc
     7441 attgcccgca tcacgaaaaa gagcataaaa ccgatgatc
//
LOCUS       DAZOYL010000197         7471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZOYL010000197 DAZOYL010000000
VERSION     DAZOYL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.198"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            275..1228
                     /locus_tag="ACHUOY_002982"
     CDS             275..1228
                     /locus_tag="ACHUOY_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0683707.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEEELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            1228..1524
                     /locus_tag="ACHUOY_002983"
     CDS             1228..1524
                     /locus_tag="ACHUOY_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683708.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            1848..2138
                     /locus_tag="ACHUOY_002984"
     CDS             1848..2138
                     /locus_tag="ACHUOY_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683709.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            2359..2574
                     /locus_tag="ACHUOY_002985"
     CDS             2359..2574
                     /locus_tag="ACHUOY_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683710.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            2721..3026
                     /locus_tag="ACHUOY_002986"
     CDS             2721..3026
                     /locus_tag="ACHUOY_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683711.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            3502..4212
                     /locus_tag="ACHUOY_002987"
     CDS             3502..4212
                     /locus_tag="ACHUOY_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0683712.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            4237..4443
                     /locus_tag="ACHUOY_002988"
     CDS             4237..4443
                     /locus_tag="ACHUOY_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683713.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            4542..6722
                     /locus_tag="ACHUOY_002989"
     CDS             4542..6722
                     /locus_tag="ACHUOY_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0683714.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            6879..7471
                     /locus_tag="ACHUOY_002990"
                     /pseudo
     CDS             6879..7471
                     /locus_tag="ACHUOY_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640578.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
BASE COUNT         2458 a         1318 c         1608 g         2087 t
ORIGIN      
        1 aatttataat cacatctgtt atagttttta tatggggtga taaaagaact cagaacatta
       61 agaggttaca acgttgggag gcgtcagggt attgacagat tttcacggcc tcttattcaa
      121 tcaaggcttt tccttttgct gttaatactc gcgtaatcgc tcaggcttct gagttccttt
      181 atcagtccat cttctaatta attcactccc tctggggttg gtcactatac gtggtcagcc
      241 ctttctttgt tttattttta aaaaggaaaa tataatggaa aaattccaca tgtttgatga
      301 ccgtatttat cagatgacta atggcgttaa agctgccgac cgtattattt acactgtcat
      361 gttgaatgaa caaaagtttt atcacgataa ggggcaatat tatacccctt catatgctca
      421 tcttggtcag tatgctggaa tcacaagaac atccgtgatt gcatgtatga aacaacttga
      481 ggcacatggt ttaatcaaga aaactaacgt tgcaagcaat cagtgtgtta ttaaagttta
      541 ttcacttgat gaagtaccgg aggttttaac agagcttaca gctaaggaag ccagaaaggc
      601 aaagctggag tctaaagtta ctcagtcagt caagaaagat gacccactga gtcaaaattc
      661 tttacccact gagtcaaaat tttttactca gtcagtcaaa atctttgaca cgtataataa
      721 cagtaataac aagaaagaac ttattaataa caataaagaa gatgactttg aagaagaata
      781 ttttaagaag ggagcttcgc tctgtgattc acatgaatgt gattctcttg gtattgaaga
      841 ggctattaaa gagactgtta aaacatctga cttagatgac cttgatagtg aagctgctaa
      901 agaggtcgtt aaaaaggaag ataaaatctc tgatgtgatt gctagtagtg accataaaaa
      961 cacacagtgc cccgccactg atgatgaatt accggatgtt gaccttgatg atgaagaaga
     1021 ccctgaagag gaattgaaac gtgaacagcg taaagcagaa attaaagaaa gattcagaaa
     1081 tgcgaagcta aagaacaagg ttagtgacct cgatgacttt gatgatagtg acgatgattt
     1141 tacttttaca ccaaaaaaga aaactcacac cgagccaaag aaagaaaaag ttaaagtcag
     1201 acgtgtttat ggtggaggaa tgttttaatg aaaacttata atggcgagag aaaataccgt
     1261 gactatttac tcagacgtta cagcatttct cgtgaaggtt acttgatgaa agatactcac
     1321 ggggaagttt accggattcg accgaagaaa gaagggaggg attattattt cgtcgatgat
     1381 gtgacagatt taaagattga cgctttgaag tttgcggtgc tgtatcatta cgatttatgg
     1441 gattcagtcc accagctacg attaaaagac ggtgaccccg gtaatctgag ggccaccaat
     1501 attatcaagg gaaagtgccg gtaaggccac cagctattta tttactgaaa tctctatctc
     1561 aacacgaaga agtttaagtc gaattgtaag ctttgtcttt tttgtaatga ctcgattcat
     1621 aacgcaaaac accaatagca caataaatac cgcaaagccc aacgagctaa aacgatgaga
     1681 ttcaataatt gctgtgaaaa aatcatgcat taattttcct tttgtgaaag gaaacagcac
     1741 ctgtatcagc ccctacaacc atagtatcat gtaaatgagg tactgacaat atgttaaata
     1801 gatgttaaga ttgtagtatg agtaagagat ttaaggagat ttttacaatg actcacacag
     1861 ttacgcgcct tttgccagag gcatttgaac gactggcagc aatcaaagcc aaatatcccg
     1921 atatgaagca cacagcgctt gttggggaga ttttaaaaga gtcggtaaac cttagccctt
     1981 tcgataacgt taagggcgtc gcagagggtt ctaaggccat cagaatcaca acgggccaac
     2041 tggaactact caaagcacag tccgcagtag caggttgctc aatgattcag gtgttaagcg
     2101 ctaaggtcca tcaactggcg gatagattgg aggtttaatg tgttataggt tccaatattc
     2161 cacacttggc cgccacctaa tcagacagaa tttagcagat gctgaatttg aagatgtgac
     2221 cctcaaagca aacaaacgat taaaaccctc tcaaatccac cagcttaaac acgaattaaa
     2281 gccactggta atactcgact ataacgaccc tgaacagctt gagcagggaa ttaagcttat
     2341 ttctgaatgg ggtgcttgat gaaaactatc acagtacaac ttcagactaa taaagcattt
     2401 cgttattttg aaaacttgct tgagttatac gaaggatggg gaagtatcca cggcaaagat
     2461 gatatctatc ttcacttgtc agcccctaac tattctctga agactccggt taagcaaagc
     2521 tggttaaaag actacggtca ccagatgggg ctattagtct cagacttgag ctaaaaacag
     2581 caaaacatat ctctcaggct ttaccagacg caccagaaac gtttttaagc ccctaatcag
     2641 ggtaacttag ggaatcgcct taagtggtct aaaaaagggg ttaaaacgtc ttagaatgcg
     2701 atacaggaag gattttaaaa atgaattacc caatgcaaat tatggttgac caagaaacca
     2761 tgaattggtt gattgacctg aacaggtcgg gctttaatat ctatttcatg cattgttttg
     2821 aagcgtcaac gtatattgac aagcatataa tcaacgaaat taaacttggt gactatgttc
     2881 acaagctgga agtaacagac ccatacaata agcgctttat cattgaagag tgcaatcgac
     2941 ttgatttaag ccctgagcaa ttattgaagc tgaatgtatt cttgacctat cagaatgaat
     3001 taaaccctta cagcgaggtg atgtaatgac actcgttatt ctctggatga ctttagggtt
     3061 atttgttggt tgctgggtag gtaatacggt aaataagaat tgatgttacc aatctgttaa
     3121 aaagctgttg acaaataacc ttaagcatga gatgatggta atagggaaga gcaactcttc
     3181 ctgaattaaa ctattaatca ttctacatca atgatagtta ttctgaaagg tgcaatgctt
     3241 ttcgctacat gacctttcag gttagtctga gaggtcatct cgaaaagtac tcttgttcat
     3301 tcgtttgaat cctttttgaa tcctttagat gttttaattt cttcacctta tgaatggccc
     3361 tttataagaa attatctagg gctattcagc ttttaaaaag catacttggc aaaccgcctt
     3421 ttaagtctct tctaagttta gataggctgt caggtgtgct ttttaaaaac tgaaacactt
     3481 ccgcttagaa gaggaaaagc aatgacaaat gatatttaca ttaatggcaa agtgattgat
     3541 agcttcagtg actggtcgcc acagactgaa catccagcta tcactattca gcgtaaagag
     3601 catgacaagc gtattgagct tgagcaatcg ctaaagatgt caccaaaaac aaccatcttt
     3661 gtgtctcctg agcctcaaga gatgcctgat atttgcaaga gtgaagcatt acttgagctt
     3721 gaaaagaaat attaccaact gctgaaagaa cagcgcctta gattagatga cgcttacagc
     3781 aaagttacct tgatgcaatc agctattgag ccgtcagaat ttgagattca ggatgaattg
     3841 tcacagaaac catttgtcta ttaccaatac gaagataatg atggttttgg tacttttcaa
     3901 gaaaacgtcg cagccgtgat tagtagtctt cctgctggac atcgaattgt gaagatggtt
     3961 aaggcatcaa gaggtgctgg tcagtttatt tacatgacta ataagagcga tgaagaactc
     4021 gctgaaattg ctagacagaa catcttggca tcacgtaaca agcaacttga taatgttaag
     4081 ttgtatctct caagagagct tcaggctatg aaagagcttg tgaaagaata tgaagatgct
     4141 aagaagattg ctatgcaggc tgatattgag cagcttacga agatttctag caaatatgca
     4201 aaggctattt aattaataat taaaaggtgg tggtgaatga ttgatttaac agaacgctat
     4261 gagcttatta aagcaattag tgaacgattg aaagaacaac ccaacaaacc aaaacgaatc
     4321 aaatcattca cctcctctaa gcaaaagaat agaatcagga aaatagctaa gtggtgtctt
     4381 gataaattac caagtgattg tcagattgtt ggtgaagatg gtaattatta tctggttagg
     4441 taacaagatt ggttgggtga aaatctacgg gagagattat cgagtgatat cggtagtctt
     4501 tccctttttc gtatatgaac acttaaagag gtgaaacatg aatgagcaaa ttcacagttg
     4561 atagttttat cgttgaactt agtttcagtg aaaacgttat taagggcttg caaagagttg
     4621 aaaaggcagc tttgcagtca gcacaaagaa ttgaacgcaa tttgaataga gcgtttaagg
     4681 tcgataccaa gcaacttgac agcaatctga caagttcttt agggcagctt gaaaggaagt
     4741 ttaataagac ctttgacaag attgagcaga gagcaagaag cacaaaagca tttcagtttg
     4801 ccaccagatt taatgacact gttaatcctc caaaacaatc aaggcaacca cgaattagcg
     4861 gaaacagagc aatcacagca gcgcatagcg tgaatatgtc taagcttggt gatattaatc
     4921 cgctgatggc taaaatgttt aaagcacagt attacagctt aagcggtaag gctggtaata
     4981 ttggaagtga aaagtttaac gctgaacttg caaaattaaa tcaatctctt cgtgaaacgt
     5041 tgaataaact caggtcacag acaaagaccg ccagcattaa caatacaagc gacgcattta
     5101 ataatcttgc aagcaatgcg attaaattat ctggtgcatt ttactctgtg atgggtgctc
     5161 ttaacgctta caaggccatt atgaatgcag gtcttaaacg agattccgca cagagagccg
     5221 ctaagtttgt acttggtgat aaagcatctg aagctgaggt atttattcga aatttagcag
     5281 acaagacagg tcttaatatc tctgaagggc tatcaagcta cgctaagttt gcagcaggtg
     5341 ctcagggttc tatgtcacag gaacagacac aggagctatt tggtaatgca acggctatga
     5401 gtcgcttaat ggggctttct aacgatgagc taaacggcat tttgagagca tttgagcaga
     5461 tggcgagcaa gggcaagatt caggctgagg aattacgtgg gcagttaggt gaccgtatgg
     5521 ctggtgcatt taagctattc gctgaagcat tgggtatgac tgctacagag cttgatgcag
     5581 ccatgaaaaa cgggaaggta ctttccgctg atactctccc taaagtcgct aaacagatgg
     5641 gcttgatgat tgataaagcg ggtggctggg ctgaagtcac taagagcact cagacagctt
     5701 taggtaagtt agcaaacaat tgggatgata cgatggtgaa aatcttctca ggttcacagg
     5761 atgagctaaa cggcttctta tccagcctga gtaacctgct tagtgaaatg gggatgagtt
     5821 cttctatcgc tggtgatgct atcggtggac ttatcgacat gcttaaagct ggtgtagatg
     5881 atattcgcgt attcaataac accattgaag gctggatttt acagactaag aagttctatt
     5941 acagccttga tgacactaaa cgaaaactac ttgatgaagt cggtgatggt tttatcaact
     6001 tcgtcaaagg tcttgctata gcgttatctg caaagacgct attcagtgct actacagggg
     6061 ttatgaacct gacaagagca atcacaacgt taggtacaag agctaatcag gtggctggtc
     6121 aggtagctac tggtaaaggt ggtggaaaac tgaaaggtgt agctggtggt gtaggtagtg
     6181 ctcttgcaat tggttatgct gatgacggtt atgaaacagc gttagcatta gcttcaatga
     6241 ttccacaaat cagaggcgtc actcttggtt tatatgcatt gaagaaagca ctggatttta
     6301 tgaatcagga agtagtcaaa aacgctaata tgcatccgtc tggggtaggt gtgggtagtg
     6361 actttaatcc cgtgtattct ggtgacccta ataaaccgaa tatgattaac gaaggatgga
     6421 gtagaatttt tagttcaatg ggtgaattgt ttaacaatgc cacaatgaat attgcaagat
     6481 tcaatcatcc agagttaaac aacattaagc aagattcagc attaacgagt gcagatattc
     6541 agggtctgag ggatgaaatt agtgctttga gtaagcgtat tcaggagcca gtgaaagttt
     6601 cacttggtgg tcaagttgtc gttcgaccag atgaaccatc tttctttact ttcaatcaat
     6661 cagctaatga ccagttcacg caggatatgc tcttaagttc aagctatcca gaggaagaat
     6721 gatttaacgg tgttctacgt gaataaagca aagttaatac ccacaccaaa aattattttt
     6781 cacgtagctc acgcttaaca gagaaaatac atatatatca aaggcttggt taaaaatgat
     6841 taaaatacac taagcctttg aaatcaaagg agaatttaat gatttacact aatcagcttc
     6901 gttacagtgg caaagatggc atctatttcc acttaagaga caatgtagat gccttcttaa
     6961 ccttatcagc aactgaaaac atggaatttg atagccctat gcaggtgact acacagaaca
     7021 tgcaattatg aaggaagctt attattaact cgtcagggtc aattagtaga aaacttcatt
     7081 gacactctgg aaaactggcg tgaccagaag cagattattt ctgttatctg taaagatgga
     7141 attaaaattg acgattccat tattaccaac tttaaagcct ctaaagatgt tactatctct
     7201 aacggtcttc gtatccagct aacttttcag gaaattaact ttaaagcaat cgtaggtcaa
     7261 actgatattt cagcagccac tggcaaaacg gctaccacaa atgacggtgg tgtgaccagt
     7321 aagaagaatg caggtaacgt gaccactgaa cgcaataacg cgcctatgct atcgtgcaaa
     7381 gagttaatga atattagcga tgtatccggt tacgctccag ctatcagaaa tgatattctc
     7441 gatgctcaga ttaaatgtaa taaggcttgg a
//
LOCUS       DAZOYL010000198         7416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZOYL010000198 DAZOYL010000000
VERSION     DAZOYL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.199"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..733
                     /locus_tag="ACHUOY_002991"
     CDS             <1..733
                     /locus_tag="ACHUOY_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFR0683715.1"
                     /translation="MIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQVLNPMN
                     IAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAFEQNISA
                     LALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRIIDTKKIN
                     SRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKVAQLEKL
                     RGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(763..1767)
                     /gene="dppF"
                     /locus_tag="ACHUOY_002992"
     CDS             complement(763..1767)
                     /gene="dppF"
                     /locus_tag="ACHUOY_002992"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR0683716.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(1764..2747)
                     /gene="dppD"
                     /locus_tag="ACHUOY_002993"
     CDS             complement(1764..2747)
                     /gene="dppD"
                     /locus_tag="ACHUOY_002993"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR0683717.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(2758..3660)
                     /gene="dppC"
                     /locus_tag="ACHUOY_002994"
     CDS             complement(2758..3660)
                     /gene="dppC"
                     /locus_tag="ACHUOY_002994"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR0683718.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(3670..4689)
                     /gene="dppB"
                     /locus_tag="ACHUOY_002995"
     CDS             complement(3670..4689)
                     /gene="dppB"
                     /locus_tag="ACHUOY_002995"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR0683719.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(4997..6604)
                     /gene="dppA"
                     /locus_tag="ACHUOY_002996"
     CDS             complement(4997..6604)
                     /gene="dppA"
                     /locus_tag="ACHUOY_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR0683720.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(7349..>7416)
                     /locus_tag="ACHUOY_002997"
     ncRNA           complement(7349..>7416)
                     /ncRNA_class="other"
                     /locus_tag="ACHUOY_002997"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         1716 a         2043 c         1906 g         1751 t
ORIGIN      
        1 gatgatcccg cactggaatt tcgccaatat aaccgccttc ccgcaagcct ccgtcttttt
       61 ccgcgatgtc ttgcttacca ttccattttg cttcttttct gcagtattta ttcaggtact
      121 taacccaatg aatattgcct atcgtaaacg ggaagcggat aaagtactgg caacccggct
      181 cgcgctgcgt acccaccgaa ttagttatat cacgctcatc gcggtgatcc tgttttttgc
      241 cttttcgttt accttctcaa ttagccacga agaagccgtt tctgcctttg aacaaaatat
      301 ctcagcactg gcgctggccg cgcaggtgat ccctgggcat atcattcata tcacctctac
      361 ggtgcttaat atctttgccg tactgaccgc attctttggc atttatctcg gtttccacga
      421 ggccattaaa ggcattattc tcaatctgtt aagccgaatt attgatacca agaaaattaa
      481 ctcacgcgtg ctgactctgg cgatctgcgc ttttatcgtc attacgttga cgatttgggt
      541 ttcgtttcgt gtatcggtgc tggtgttctt tcagttggga agcccgttat atggtattgt
      601 gtcgtgcctc attccgtttt tcctgatcta taaagtcgca caactggaaa aacttcgcgg
      661 atttaaagcc tggctgattc tgctgtacgg cattttgcta tgcttgtcgc cactgttgaa
      721 gctgattgag taaaccggag cgcatggccc cggttttgtg agttaacgct gcggattttc
      781 atcctgatca acagcaaaac aagctaccag ttgaccgccg tagtctttta gctgcggctg
      841 caactgggtg caggggccga agcgccgacg acagcgggcg ttgaaggcgc aacccggcgg
      901 tggattcagt gggcttggta gttcaccgct gagcttgatg cgctcgcggc gatcgtccgg
      961 gttcaggcgc ggcgtcgcgg aaagtagcgc ctgagtgtac ggatggcgcg ggttattgaa
     1021 gatttggtct ttcgttccct tctccacgca gcggcccagg tacatcacca tcacttcatc
     1081 agcaatgtgc tccaccaccg acaggtcgtg ggagataaag acataagaca gccccaactc
     1141 ctgctgcaaa tccatcatca gattcagcac ctgcgcgcgc actgaaacat ccagcgcgga
     1201 aaccggttca tcggcaatca ccacatccgg gtcgagcatc agaccacggg cgatggcgat
     1261 acgctgacgc tgaccgccgg agaacatatg cggatagcgg tcatagtgct cggttttcag
     1321 gccgactttc gccatcatcg acagggcttt ttcccgacgc tgttctttgc ttaagctggt
     1381 gttgatcagc agcggctctt caagaatttg cccgactttt ttacgcggat tcagcgaacc
     1441 gtaagggttc tggaagacga tctggatttt ctgccgacgc agcttctgcg cctgcggatc
     1501 gtgcttaagc agatcctgcc cctggtaata cagctcgcca ccggtgggcg tttcaatcat
     1561 cgtcagcaac cgaccgaggg tcgatttacc gcagccagat tcgcccacta ctgccagcgt
     1621 tttgccacgt tcaaggttaa acgaaacgcc atccagcgct ttaaccagac gttccggcgc
     1681 gaacatgcct ttcttcaccg gataatgttt tttcaggtcg atagcctgca acagcggttg
     1741 ttgcagggtg gcctcttgcg tactcatagt gtcggcctcc cggcatcatc aagtgggtaa
     1801 tggcatttgg actggcgccc gtcagcgagc atattcagcg ccggttcttc agcgcgacat
     1861 ctgtcagtgg cataggggca gcgcgggtta agcaggcagc cgttcgggcg gtcgtacttg
     1921 ccgggaacga cacctggcaa cgacgccaga cgttctttgt cctgagcaaa ttctggcagc
     1981 gcacgcagca atgcctgagt atacgggtga cgcggcgcat ggaagatggc gtgcgcatcg
     2041 cccgtttcca ccacctggcc tgcatacatc acaatgattt tatgcgcagc ttccgccacc
     2101 agcgccagat catgggtaat taacaccagc gccatgttct ctttctgctg tagctccagc
     2161 aacagttcga tgatttgcgc ctgaatggtc acgtccagcg cagtggtcgg ctcatcggca
     2221 atcagcagtt ttggccgaca ggcaatcgcc atggcgatca tcacgcgctg gctcatgccg
     2281 ccggaaagct gatgcgggta aacatccaga cgcgatgccg gatcgggaat accgacctga
     2341 ttcagcaggt caatcgcccg ctgacggcga gtgctcttat taccgccctg atgcacctta
     2401 atcgcttcca tgatctggaa acccacggtg tagcacgggt taaggctggt catcgggtcc
     2461 tggaagatca tcgccacttc ggcacccacc aggttgcggc gctctttttc tgatatacgc
     2521 tgcaaatcct gaccgttaaa caccagcttt tccgccatta cgcggcccgg ataatcaatc
     2581 agccccataa tcgccagtga actgaccgac ttaccggagc cagactcacc cacaatcccg
     2641 accacttcgc cctgttttac gctgtagctg atgcggtcta cggcgcggaa cggtgcgctt
     2701 tcgtcgccga aatgcaccga taatttatct acatttaata acgccatctc gaacctctta
     2761 ctgcttcagt ttgggatcga gcgcgtcacg cagaccgtca cccatcaggt taaatgccag
     2821 caccgtcagc aggatcgcca gacccgggaa ggtcacgacc caccaggcgc tttgcgcgaa
     2881 ctgcaacacg tcggagagca tggtgcccca ctctggtgtt ggcggctgcg ctcccatgcc
     2941 gaggaagcca agggcggcca tatcgagaat ggcgttagag aaaccgagcg acgcctgaac
     3001 aatcagcggc gccaggcagt tcgggaagat gttaataaac atctgacgca tcgccccggc
     3061 acctgccacg cgagacgcgg taacgtaatc gcgattcacc tccaccagca cggcggcgcg
     3121 ggttaagcgc acatagtgcg gcaaggcaac gaaggtcagt gccagcgcgg cgttaccaat
     3181 cgacgggccg aaaatcgcca ccagcaccag tgccaacagc agacttggca gcgccagcat
     3241 gatatcgacc acgcgcatga tgatgttatc gaccaggccg ccgaagtaac cggcgatcag
     3301 gccgagaata acgcccataa tcagcgataa caccaccacc agacagccaa ccagcagcga
     3361 caagcgcgcg ccgtacatca gccgcgacag cacatcacgg cctacgtcat cggtgcccag
     3421 caagtgcgcc atgctgccgc cttcctgcca ggctggcggg gcgagcagcg catcgcggaa
     3481 ctgatccgcc gggttataag gcgcaatcca gttggcaaag atcgtgataa acagcacgat
     3541 gacgatgtaa accagcccga caaccgcgcc tttgttgcgt ttaaaatagt gccagaactc
     3601 ctgtaacggg gtcatcggca ccggtgcgct aatcacttta ttttcagtaa cctgtgacat
     3661 gatgtcccct tacttcttat gacgaatacg cgggttcacc acgccgtaca gcagatcgac
     3721 cagcaggttg acgaggataa tcatcgtcgc caccagcaat acgccgccct gcactaccgg
     3781 atagtcgcgg cgttgcagtg cgtcaatcaa ccagcgtccc agaccgggcc acgagaagat
     3841 ggtttcggtc agaatcgccc ccgccagcaa tgttcccacc tgcaggccga taacggtcac
     3901 taccggcagc atcgcgttac gcagcgcatg gacgataatc acccgcatgc gggtcaaccc
     3961 tttggcgcgc gcggtgcgga tgtaatcttc acccagcact tccagcatcg aagagcgcgt
     4021 catacgaacg ataaccgcca gcgggatagt acccagcaca atagccggta agatcatatg
     4081 ggcaacggcg tcgataaagt tgccatcttc accccagatg gcggtgtcga tcagcataaa
     4141 accggttaac ggattggagt catcgaggaa caccatatcg ctcacgcgac cggagacggg
     4201 cgtcaggttc cagtgcaccg aaaccagcat gatcagcatc atgccccacc agaagatcgg
     4261 catcgagtag ccggtcagcg ccaggccaac tgcagtgtga tcgaaaatgg aaccgcgttt
     4321 aaccgcagcc agcacaccaa ccggaatacc gactgccgta gcaaaaatca tcgcgcagac
     4381 gccaagttcc agcgtggcct gaaagcgcgg cacgaactct tcccatactg gaattcggct
     4441 tttcattgaa atgcctagat cgccgtgcat tacgccccaa atgtaatgga gatactgctg
     4501 ccacatcggt ttatctaagc cgagttcagc cagcagctgc gcgtgacgct caggggagat
     4561 cccgcgttcg cctgccatga tcatcaccgg atcgcccggg atcatgtgga caaaggcaaa
     4621 tgtgagaagg gtaataccga taaacgtggg gatgacgagt cccaaacgtc ggagaataaa
     4681 ctgcaacata acccggattc tctatagtga cccgcagcct ggaactgcgt gtctgtattg
     4741 ctcacaaatc aattcccgcc cttatcggac agggaatgaa gtattgctgc cggatggcgc
     4801 tgcacgcaga ggcacatcgc caccgggtat acagattgtt gacaaagtgc gctttgttta
     4861 tgccggatgc ggcgttaacg ccttatccgg cctacaaaat cgtgcaaatt caatacattg
     4921 cagaattttc gtaggcctga taagcggagc acatcaggca gttttgcctt tgccatcagt
     4981 cttgtatggc ttttaattat tcgatagaga cgttttcgaa gtgatgtttg cctaatggat
     5041 caaccacata gcctttgact tctttgcgta ccggttcaaa cacggtggag tgagcgatga
     5101 tcagtgccgg agtctgatcg tgcatcacca cctgcgcttg cttgtacagt tcaacgcgtt
     5161 tgttatggtc gtcggtagca cgcgccggtt gaatcagatc ttcaaacggt ttgtagcacc
     5221 attttgagta gttagaacct tgttcagagg cggcgcagct gaacagggtg gcgaagaagt
     5281 tatccgggtc cccgttatcg ccagtccagc ccatcattac cgtctgatgt tcgccatctt
     5341 tcgcacgctt gaggtactca ccccattcgt aggtgacgat tttggcctgc acgcccactt
     5401 tcgcccagtc tgcctgaatc atctccgcca tgcggcgagc gttcgggtta tacggacgtt
     5461 gtaccggcat cgcccacagg tcgatggaga aacctttttc cagacccgct tctttcagca
     5521 aggctttcgc tttttcagga tcgtaggtgt aatcctgaac gtcgtcgtta tagccccaca
     5581 tggttggcgg gatcaggttt ttcgctgata cacccgcgcc ctgataaacc gctttgatga
     5641 tcgcgtcttt gttcaccgcg taggtcagtg cctggcgaac tttcacgtca tccagcggtt
     5701 ttttctgcac gttataggag aggtagccga cgttcagccc cggcatttcc atcaggttga
     5761 tggatttatc ctgcttcatg cgagcgatgt ctgccgggtt cgggtacggc atcacctggc
     5821 attcattctt ctgcaatttc gcgtaacgca cggaagcatc aggggtaata gagaaaacca
     5881 gcgtatcgat ctgcggtttg gtgccccagt agccatcaaa cgctttgtag cggatacggg
     5941 aatctttttg gtactgctgt aactggaacg gaccggttcc gattgggttg aggtccagtt
     6001 tttccggtgt accggctttc atcatcgcat cagcatattc ttttgacaga atagaggcga
     6061 agtccattgc cagatcagcg aggaacggcg cttccgggcg agtcagtacg aactgaactg
     6121 tgttgtcgtc cacctttttc acttcactga tcagctccgg caagcccatg ccttcgaagt
     6181 attcgtagct gccgccagag actttatggt acgggttttg cgcgtttttc tgacggtcga
     6241 acgagaacac cacgtcatcg gcgttcagtt cacgcgtcgg tttgaattct ttattgtcgt
     6301 gccacttcac acctttacgc agatggaagg tataggtttt accgtcttcg ctgacttccc
     6361 acttttcagc gaggcccggg atcacttcag tggtgccgat tttaaattca accaggcggt
     6421 tataaagcgg tacggaagag gcgtcatagg tggtgccgga ggtaaacagc tgcgggttaa
     6481 acccttccgg agatccttct gagcaataaa ccagagtttt agcctgaaca cttgctgcga
     6541 cggtcatagc caccaggctg agaccaagct tcagcatccc tgactttttc aaggaaatac
     6601 gcattattct gctccaattg tgatgtttgt tgttttaacc ctttgcagtg ggttgtcgct
     6661 gcctgacctt ttttgttttt tgcccggtcg ggtcaacgtt atgaggtggg gatgccgtac
     6721 taattaacat cagtgtaaca gtgcggatcc tccataaaat gcccctcgtg acctacaatc
     6781 tgtcaacaga atgtgaaaac gtcaatacag ccaaccggga tttacaccaa cggtgagaat
     6841 ccacaaacaa agattaaaaa aacttcaaac agctatttgc agcagagaaa tttgtgctac
     6901 tccaacataa ccagaacaat cagcttaata tttagcaaca tttggtgatt aagttttatg
     6961 catttaatga aaaaaatctg aggaaaaggt ggatatctga gcgattaata ccatgaacgc
     7021 taaaacgcac agcggaaaat gccagcgcca gccataagta acgcaaaaaa agatttaagc
     7081 aaatataaaa aaagacaatg gtttctgtca caaatccgtt aatggatagt gagatatggg
     7141 gcgcaaagtt ggcaggaaaa tgtgtggtgt gttggtcgaa taaggcggtc gcgccgcatc
     7201 cgacaataaa tgcggtgccg ttgcctgatg cgacgctggc gcgtcttatc aggcctacgt
     7261 cgagtcgaac ctccctgccc ccggaggttc tcatcctcat gagctacaaa tgcaaaaaag
     7321 cctgctcgtt gagcaggctt ttcgaatttg gtcggtgata gaggattact cgccacttcg
     7381 tggctcgccc tgcgggccgt tgctggcgca acgttc
//
LOCUS       DAZOYL010000199         7363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZOYL010000199 DAZOYL010000000
VERSION     DAZOYL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.200"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            119..379
                     /gene="dinJ"
                     /locus_tag="ACHUOY_002998"
     CDS             119..379
                     /gene="dinJ"
                     /locus_tag="ACHUOY_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR0683721.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            382..660
                     /gene="yafQ"
                     /locus_tag="ACHUOY_002999"
     CDS             382..660
                     /gene="yafQ"
                     /locus_tag="ACHUOY_002999"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR0683722.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            816..1556
                     /gene="dpaA"
                     /locus_tag="ACHUOY_003000"
     CDS             816..1556
                     /gene="dpaA"
                     /locus_tag="ACHUOY_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR0683723.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1527..2294)
                     /locus_tag="ACHUOY_003001"
     CDS             complement(1527..2294)
                     /locus_tag="ACHUOY_003001"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR0683724.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2500..3078)
                     /gene="lpcA"
                     /locus_tag="ACHUOY_003002"
     CDS             complement(2500..3078)
                     /gene="lpcA"
                     /locus_tag="ACHUOY_003002"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR0683725.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3318..5762
                     /gene="fadE"
                     /locus_tag="ACHUOY_003003"
     CDS             3318..5762
                     /gene="fadE"
                     /locus_tag="ACHUOY_003003"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR0683726.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5805..6251)
                     /gene="ivy"
                     /locus_tag="ACHUOY_003004"
     CDS             complement(5805..6251)
                     /gene="ivy"
                     /locus_tag="ACHUOY_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR0683727.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6432..7202
                     /gene="yafV"
                     /locus_tag="ACHUOY_003005"
     CDS             6432..7202
                     /gene="yafV"
                     /locus_tag="ACHUOY_003005"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR0683728.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(7244..>7363)
                     /locus_tag="ACHUOY_003006"
     CDS             complement(7244..>7363)
                     /locus_tag="ACHUOY_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0683729.1"
                     /translation="AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         1752 a         1849 c         1960 g         1802 t
ORIGIN      
        1 atatatttca gtttattatg taaaaacatc cgtttattaa attactcatg gattgtcgct
       61 gttgctcatt tgagctacaa ttcaagctga ataaatatac agcacaggag atcccccaat
      121 ggctgctaac gcgtttgttc gcgcccgaat cgatgaagat ctgaagaatc aggcggcgga
      181 cgtactggcc gggatggggc tgaccatctc tgacctggtt cgcataaccc tcacaaaggt
      241 cgcgcgtgaa aaggcattgc cgtttgattt acgcgagcct aatcaattaa ccattcaatc
      301 aatcaaaaac agcgaagctg gcgttgatgt tcataaggcc aaagacgctg atgatttatt
      361 tgataaatta ggaatttaaa tatgattcaa agggatattg aatactcggg acaattttca
      421 aaggatgtaa aacttgcaca aaagcgtcat aaggatatga ataaattgaa atatcttatg
      481 acgcttctta tcaataatgc tttaccgctt ccagctgttt ataaagacca cccgctgcaa
      541 ggttcatgga aaggttatcg cgatgctcat gtcgaaccgg actggatcct gatttacaaa
      601 cttaccgata aacttttacg atttgagaga actggaactc acgcggcgct ctttgggtaa
      661 agcacgctgc cgatgaaatt tgtaaaccct gcgccgacag gcttgcgtaa aactcttttg
      721 gtgataatgt gagccttcca aatccagact attccgattt gggatctatt ttgccgtcct
      781 ggcgtgtgta accgttttat caaggaataa gcagtatgcg taaaatcgca ttaattcttg
      841 cgatgttgtt gatcccgtgc gtttcgtttg ccggtttgct gggcagcagt agctcgacca
      901 cgccagtgag caaagagtat aagcagcagt tgatgggatc ccctgtctac atccagatct
      961 tcaaggaaga acgtacgctc gatctctacg tcaaaatggg cgagcaatat cagctgctcg
     1021 acagttataa aatctgtaaa tattccggcg gcttagggcc aaaacagcgt cagggcgatt
     1081 tcaaaagccc ggaagggttt tatagcgtcc agcgtaatca gttaaaacca gacagccgtt
     1141 actacaaagc gattaatatt ggtttcccca atgcctatga ccgtgcgcat ggttatgaag
     1201 ggaaatacct gatgatccac ggcgattgtg tttccatcgg ctgctacgca atgaccaatc
     1261 agggtattga tgagatcttc cagttcgtta ctggtgcgct ggtgtttggt cagccgagcg
     1321 tgcaagtgag tatttacccg ttccgcatga ccgacgccaa tatgaagcgc cataaatatt
     1381 ccaactttaa ggacttctgg gagcaactga agccgggtta cgactacttt gagcaaaccc
     1441 gtaagccacc gaccgtttct gtggtcaatg gtcgttacgt ggtcagcaag ccgttaagcc
     1501 acgaagttgt gcagccacaa ctggcatcaa actacacgct ccccgaggca aaataagcgc
     1561 cattcgcctg gcataatctt ttgccaggtt tcattgcccg tcagcggctg tgttgcaatc
     1621 accgtgacca catcattcgg tgtggtctgc gagctgaagt cgatttccac atcctgatcc
     1681 agcagcgttg ccacgccaaa cggcgcgcgg cgggtgatcc agtgtaaatt agtcgagcaa
     1741 tacgccatca catagcgccc gtcagaaagc agcatgttga aaacgccctt ctgccgcagt
     1801 tcatccgcca gtgaggcgat atatttaaac actgccgcca tgttgcccgg tgtgcgcggg
     1861 taacgctgcg ttaatttatg caggagccag cagaaggctt tttcgctatc ggtttcaccg
     1921 accgggcgga agttgccggt ttccagtgat ttgtagcccg tcagttgtcc gttatgggca
     1981 taagtccagt tacgccccca taactcacgg gtaaacgggt gagtattttc cagcgccacc
     2041 tcgccccgat tggcctggcg aatatgagcc accaccgaac aggattttat cgggtagtcc
     2101 tggacaagtt tggcgatggg ggaattaaag ctgggttgtg gatctttaaa tgtgcgacag
     2161 cctttacctt cgtaaaaggt aatgccccag ccatctttat gtggcccggt tccaccacca
     2221 cgctgcacca gaccggtgaa actaaagcag atatcggtag ggacgttggc gctcatcccg
     2281 agcaattcgc acatgggtaa cctccacaac agcgggagcg catggccccc ggcgaagatt
     2341 gatttaagaa ttttcaggcc ggatgcggcg taaacgtctt attcggccta cggttcgcgt
     2401 acagatttgt aggcatgata agacgcatca gcgtcgcatc aggcatcagc gcacaagtgc
     2461 cggatgcggc gtaaacgcct tatccggcct acgccagact tacttaacca tctctttttc
     2521 aattaactgg atcaggatat ggatcacttt aatgtgaatc tcctgaatgc ggtcggcata
     2581 accaaagtgc ggaacgcgaa tttcgatatc cgccgtgcca gccattttac cgccgtcttt
     2641 accggtcagg gtgatcactt tcatcccctt ctcacgcgcc gctgcgatcg ctttgatcac
     2701 gtttgcagag ttaccggagg tggagatccc cagcagtaca tcgccttcgc gacctaccgc
     2761 ttcaacgtag cgggagaaaa tatcattgaa accgaaatca ttaccgacgc aggaaatatg
     2821 actaacgtca gaaatagcaa tcgccgggta gcccggacgg ttttcacggt agcgaccggt
     2881 caactcttcg gcaaagtgca tagcgtcgca atgggaaccg ccgttgccgc aggaaagcac
     2941 tttgccaccg gctttaaagc tgtctgctaa caggaccgcc gcgcgctgaa tggcgtgaat
     3001 attggcgtca tcttttaaaa agttagccag cgtttccgcc gcttcgttca gttcgttacg
     3061 aataagatcc tggtacatga ggatatcctt cagcataaat gtaatagaca aaatgcagtg
     3121 taccggatac cgccaaaagc gagaagtacg ggcaggtgct atgaccagga ctttttgacc
     3181 tgaagtgcgg ataaaaacag caacaatgtg agctttgttg taattatatt gtaaacacat
     3241 tgctaaatgt ttttacatcc actacaacca tatcatcaca agtggtcaga cctcctacaa
     3301 gtaaggggct tttcgttatg atgattttga gtattctcgc tacggttgtc ctgctcggcg
     3361 cgttgttcta tcaccgcgtg agcttattta tcagcagtct gattttgctc gcctggacag
     3421 ccgccctcgg cgttgctggt ctgtggtcag cgtgggtact ggtgcctctg gccattatcc
     3481 tcgtgccatt taactttgcg cctatgcgta agtcgatgat ttccgcgccg gtatttcgcg
     3541 gtttccgtaa ggtgatgccg ccgatgtcgc gcactgagaa agaagcgatt gatgcgggca
     3601 ccacctggtg ggagggcgac ttgttccagg gcaagccgga ctggaaaaag ctgcataact
     3661 atccgcagcc gcgcctgacc gccgaagagc aagcgtttct cgacggcccg gtagaagaag
     3721 cctgccggat ggcgaatgat ttccagatta ctcatgagct ggcggatctg ccgccggagt
     3781 tgtgggcgta ccttaaagag catcgcttct tcgcgatgat tatcaaaaaa gagtacggcg
     3841 gactggagtt ctcagcttat gcccagtctc gcgtgctgca aaaactctcc ggcgtgagcg
     3901 ggatcctggc gattaccgtc ggcgtgccga actcattagg cccgggcgag ctgctgcaac
     3961 attacggcac tgacgagcag aaagatcact atctgccgcg tctggcgcgt ggtcaggaga
     4021 tcccttgctt tgcactgaca agcccggaag cgggttccga tgcgggcgcg attccggaca
     4081 ccgggattgt ctgcatgggc gaatggcagg gccagcaggt gctggggatg cgtctgacct
     4141 ggaacaaacg ctatattacg ctggcaccga ttgcgactgt gcttgggctg gcgtttaaac
     4201 tctccgaccc agaaaagctg ctgggtggcg cagaagattt aggtattacc tgtgcgctga
     4261 tcccgaccac cacgccgggc gtggaaattg gtcgtcgcca cttcccgctg aacgtaccgt
     4321 tccagaacgg accgacgcgc ggtaaagatg tcttcgtacc gatcgattac atcatcggtg
     4381 ggccgaaaat ggccgggcaa ggctggcgga tgctggtgga gtgcctctcg gtaggccgcg
     4441 gcatcaccct gccttccaac tcaaccggcg gcgtgaaatc ggtagcgctg gcaacgggcg
     4501 cgtatgctca cattcgccgt cagttcaaaa tctctatcgg taagatggaa gggattgaag
     4561 agccgctggc gcgtattgcc ggtaatgcct acgtgatgga tgctgcggca tcgctgatta
     4621 cctacggcat tatgctcggc gaaaaacctg ccgtgctgtc ggctatcgtt aagtatcact
     4681 gtacccaccg cgggcagcag tcgattattg atgcgatgga tatcactggt ggcaaaggca
     4741 ttatgctcgg gcaaagcaac ttcctggcgc gtgcttacca gggcgcaccg attgccatca
     4801 ccgttgaagg ggctaacatt ctgacccgca gcatgatgat cttcggacaa ggagcgattc
     4861 gttgccatcc gtacgtgctg gaagagatgg aagcggcgaa gaacaatgac gtcaacgcgt
     4921 tcgataaact gttgttcaaa catatcggtc acgtcggtag caacaaagtt cgcagcttct
     4981 ggctgggcct gacgcgcggt ttaaccagca gcacgccaac cggcgatgcc actaaacgct
     5041 actatcagca cctgaaccgc ctgagcgcca acctcgccct gctttctgat gtctcgatgg
     5101 cagtgctgga cggcagcctg aaacgtcgcg agcgcatctc ggcccgtctg ggggatattt
     5161 taagccagct ctacctcgcc tctgccgtgc tgaagcgtta tgacgacgaa ggccgtaatg
     5221 aagccgacct gccgctggtg cactggggcg tacaagatgc gctgtatcag gctgaacagg
     5281 cgatggatga tttactgcaa aacttcccga accgcgtggt tgccgggctg ctgaatgtgg
     5341 tgatcttccc gaccggacgt cattatctgg caccttctga caagctggat cataaagtgg
     5401 cgaagatttt acaagtgccg aacgccaccc gttcccgcat tggtcgcggt cagtacctga
     5461 cgccgagcga gcataatccg gttggcttgc tggaagaggc gctggtggat gtgattgccg
     5521 ccgacccaat tcatcagcgg atctgtaaag agctgggtaa aaacctgccg tttacccgtc
     5581 tggatgaact ggcgcacaac gcgctggcga aggggctgat tgataaagat gaagccgcta
     5641 ttctggtgaa agctgaagaa agccgtctgc gcagtattaa cgttgatgac tttgatccgg
     5701 aagagctggc gacgaagccg gtaaagttgc cggagaaagt gcggaaagtt gaagccgcgt
     5761 aattgaataa cggagccgaa aggctccgtt tctttatccg ctaattattt aaaattaaag
     5821 ccatccggat ggttttccag gctgccggtc aacgccgcga acagcaccgt tttaccatca
     5881 atcgaaagcg catcgttcac attcagccag gtgagtttct cttgcgacgt tttctcatca
     5941 atagtcgaga acagccccgt catctgatta gatttctcgg accacatcac agcgatacgt
     6001 tgcgagccac agtcatgcgg tttgcacgcg ctcatcacct gatacgtatc atctcccaac
     6061 gttacggttt gtgcgggagt ataagtaccg cctttcatca cccaggcagg cagcttatgc
     6121 ccttgtacca tctgattaaa tgcagctttg gtggtttcgc cctttgcaag gctgctaatg
     6181 gttaaatcat cctgcgccat tgcactggtg gcgatgacca gagcggcgac tgtcgttatt
     6241 gtcttaaaca tcattcctcc cgagcttatc ctgcccatgt tattaacctt ctaaagcctg
     6301 gcgataacac gataatttgc aaatcagata tttccaatat cagcatttgt tatcaattta
     6361 ttctgcgcta aggcaactgc ctgataaggt gttaaagtta taaaaccctt cgcataaagg
     6421 agttgtacac cgtgcctggt ttgaagatta cgcttttgca gcaaccactg gtgtggatgg
     6481 atggccctgc caacctgcgt cattttgatc gtcaactgga aggtattacc gggcgcgatg
     6541 tgatcgttct accgaagatg tttaccagcg gctttgccat ggaagcggca gcttcgtcgc
     6601 tagcacaaga tgacgtagtg gactggatga cagctaaggc gcagcagtgc aatgcgctga
     6661 ttgcaggcag tgttgcatta caaacggagt ctggttcggt taaccgcttt ttgctggttg
     6721 agccgggcgg cacggtacat ttttatgata agcgtcatct gttccgcatg gcagatgagc
     6781 atctacatta taaagcgggc aatgcgcgag tgattgtgga atggcgcggc tggcgtattt
     6841 tgccgttggt gtgctacgac ttacgttttc cggtgtggtc gcgcaatctc aacgattatg
     6901 acctcgccat ctacgttgcc aactggcctg ctccgcgctc tctgcactgg caggcattgc
     6961 tgacggcccg cgcgattgag aatcaggcgt atgtggcggg atgcaatcgc gtcggcagcg
     7021 atggcaacgg ctgccattat cgcggtgaca gccgggtgat taatccacaa ggcgagatta
     7081 tcgctactgc cgacgcgcat caggcaacgc gcattgatgc ggagctgtcg atgatggcat
     7141 tgcgggaata tagagaaaag tttccggcat ggcaggatgc ggatgagttt aggttgcggt
     7201 gagtgggggt tgtgtttaag gctgggggag agtcggggta agactacgaa agcccgctct
     7261 ccgcaaggac tgacgccaga tagtttctgt ccatagctgc ttttcgcatc ttacgtctta
     7321 accctgcctt gaatacctta tcattcgtca aaatattaat agc
//
LOCUS       DAZOYL010000200         7264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZOYL010000200 DAZOYL010000000
VERSION     DAZOYL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.201"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..4518)
                     /locus_tag="ACHUOY_003007"
                     /pseudo
     CDS             complement(1..4518)
                     /locus_tag="ACHUOY_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4538..4999)
                     /locus_tag="ACHUOY_003008"
     CDS             complement(4538..4999)
                     /locus_tag="ACHUOY_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR0683730.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5027..6928)
                     /locus_tag="ACHUOY_003009"
     CDS             complement(5027..6928)
                     /locus_tag="ACHUOY_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFR0683731.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1379 a         2206 c         1958 g         1721 t
ORIGIN      
        1 ttatttgact acgtgataaa cgaaccccat ccaggaaacc cgtatgccca ctaacacgac
       61 gaccatcgcc aatatagtcc atcaaactgc tgattttaga acttgcagcc aatcccagcg
      121 gatcaaccca cccacaagga ttcggcacat accggtaagt ctgctccccg ccctgcaacc
      181 ccagcggatc actcaccagg tacattccgg tttccggctc gtaataccgg aaccgattat
      241 agcacagccc ggattccgca tcctgccact gtcctgcata cagcaggccg gggtcggctt
      301 ccgggtccag ttccccgcgc gggtccgggt agccggtgtc tgcgggcagg ctgagtgcca
      361 gcccccacag gctggtctgc cccggtcgcc agaccgtctt tccttcactg ttaaagagca
      421 tcagcgggcg gcccgtcagg tcgctcaccg catgattcgt ccgcgtcacc cactgcgggg
      481 ccaccgacgg atgcgcctgc cgtacccggc tgaactgctg gcttatcagc tcaaagccgt
      541 taaacaccag gtgccttacg ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa
      601 tactgtcgcc gtcccacaga tacgtgaagc ggattttctt ccggtcgcag cgtttctctg
      661 ttcgtctgcc gctggcgtcg tggcggtatt cccacagctc accctgtgcg ctgcaatacc
      721 cggtcagctg gtcccggctg tcccaccgga accgctctgt ttccggtcgg tagccgtcac
      781 ggtgtctggt gcggctgacc atccggcctg cggcgtcata gtcatactgc gtgtttccgg
      841 cctgtttcag ccggtggcct ccggcatact ggtcagtctc ttcacttatc cggtgcctgc
      901 ccgcagactg cgccttcagg tagccgcagc tgtcatagcc ataggactca tcgccttcgc
      961 catagcccag cccggtgcct gaggctgtca ccgccgtgac ctggccgttc cggtccagcc
     1021 gatactcccg ccgtgtttcc cgtccgtaat cttcccggct ggtgacgcct gacacgttac
     1081 ccgcgctgtc gtaacggtac tcccgtacca gtgagggaac cagcccgtcc catgtggctt
     1141 ctgccggaaa aaatccggcg cgctgactga caggctgacc ggctgcattc cagccctgtt
     1201 cacaggcaaa accccggtta ctgtgccgga gcacttcacg tcccgcctca tcacggctga
     1261 acgtcagcgt ctcatccccc ggcagaatga cctctgtcag ttctccggcg gcgttgtagc
     1321 ggtacgtggt tttcagcagg gattcaccgg ggtgtctgtc gcgttcatct tccggtggca
     1381 ggatacagcg gatgacggtg tgttcatcgg ggtagttgta ttcaatggcc cgtcggccct
     1441 gagtggtgac attcacccgc ccgcgttcgt catagccggt gcgctgtgat gtgccgtccg
     1501 ggctgaagat acccgtcagc ctgccggatt tgtcataggc atactgcgtt ttgccttccg
     1561 gcgagatgat ttctgccagg cggcctgcgg cgtcccggct gtagaggatg gtttcctcca
     1621 ggccgttaac cttggcggta cagaggccat cggcgtcata gcggtaacgc cagaccacgc
     1681 cgccccagtc acgctcttcc gtcacccggc cgcagttatc cagaacataa agatatgact
     1741 caccctgcgg gttgatgact tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc
     1801 agcggtggcc ttcaccgtcc gttcgcgcca ccggaaaatc gaacggaccg tattccatcg
     1861 tccagacaac accgttgccg tccgtccagg ccagcaggtt gccgtggcgg tcatagcgaa
     1921 atccttccgc gccggttcca gcacgttcaa tgccgctcag tttatggtgc tgctgccact
     1981 gtaaccgcac tgactgaccg ccggggccgc gcatcttcac tggcaggtcg tgaccgctgt
     2041 attcggtgtg gtgttcacgg cccatcacgt cggtgagtgt ttccggccgg ttgtggtggt
     2101 cgtactctat gcccagacgc cgttcatcct ggcggaatat tcccgacagc agcccctgag
     2161 cggtgtatgc cagccgggtc tcttcgccct gtggcgacac cagacttacc ggcaggccgc
     2221 gttcatcaca ctgctgtgtc cacgtctgcc cggcagggtc ggtgatgttg ctgagcaggc
     2281 cgcgctcatc atagcggtac tgccactccg cgccgtctgc cgccgtgtaa cggcttaact
     2341 gaccgtgtgc ggcgttgtat tcaaatttct ccacccgccc ggcaggggag tggcgggcca
     2401 gcaggtgatg gaattcatcc cattcataca tggtggtgct gccgtccggc gcttcttccc
     2461 gcagaatatt gccgtcagga tcatagtaat aacggtgaat gcccttgccg tccgtcacgg
     2521 tggtgcagcc gtcaccatag tccagcgtgg cgttgtaata gccctccgcc ccggtgacat
     2581 tcacgcaccg gccctgttca tcgtaggtga aacggctcca cgtctggtcg ttatcggacc
     2641 agcggatgat ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat
     2701 ccgcttctgt cagccgcccc cgcgcatcct gttcgtaggt cgccagcgtc tgtatgtcgt
     2761 tatccgtgcg gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt ttaagccgga
     2821 tcccgtcggt gtgactgaca cccgtgatcc ggtactggct gtcgcgctca aacgtgatgc
     2881 ggttgctggc ggtgtcgctc atcccgccca gcatgtacat ccggtcattg gggctgtgtg
     2941 ccaggcggtc atcgctggta tcgtcagtca ccgggtggcg cagacgcatt cccggaaagg
     3001 cgtcataaaa gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga taatcatcgc
     3061 ggcggcgcag aatgtactgg gcgtgatacg ggttaaccgc cgtatcactg tcaccgtcga
     3121 aggcaaatct cagcgtggct ccctggctga tgatatccac ccggtttccc tgttcacgca
     3181 cccaggcgta ttcgctccag gagtcgctcc agcccacgcc ggtcaggcca tgcagcggca
     3241 gtacagaact gtggaaacgg gtgaaggaga gggggagggt ctggcccagg gtgaagtcgg
     3301 tacgttcgtc gcagtatgcg ccggttaccg gatcgacagg atctgttacc catctgatga
     3361 ctttattggc aatacttttt aacgacttac ctgttttccc cagcaacgcg ccgagctttg
     3421 cgcccagcag cttcccgctt ttccccagca gtttcccagt ttgccgagtc tcgccagatt
     3481 cttcagtttt cccagtagtc ccgctggcgg cagaagatag ctggctatcg ttattccccc
     3541 caggatcatt ctctgccacg gcgggaactc cggctggata tccagatatg tgacctgctc
     3601 accgccaata aagacgtccg gcgaacctcc cacgatcgcc gcactgcact ccagtttgtc
     3661 gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctgctgccct gggctatcct
     3721 cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac
     3781 atcttttttg atttcgccac gcccgcccac catggtggca aactccatta ataccggacc
     3841 ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg cattgctgtc
     3901 cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt tttccgctgc
     3961 cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg ggacttcagg
     4021 tattgctcca ggtgggggag gagggggaag ggtgaccgct gcacgtgcgg ccagctcgcc
     4081 gttaattttt acgttttcag aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt
     4141 tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct tatagctggc
     4201 atacatcagc gcaggtacca gaaccagcgc ataagggccg gtggcacagg cggccataat
     4261 caggcaacct tctgcgatgg tcactatcgc ccccagtacg gcccctgcca gcgcccccaa
     4321 aaaactcttg tgctgaattt catcgcccag cctggcggcg ggggggccag acttttttga
     4381 tgccaacatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc tggcgaccat
     4441 cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc cctgtggccc
     4501 gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg tccttttgga
     4561 tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc atcgtctttt
     4621 gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta tctccacgcc
     4681 gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc ggaccgttcc
     4741 aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg caggcatgtt
     4801 cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac agctctggtg
     4861 tcagggttcc ctgtggaacg gcttcgcggg taatgaccag attgatgcca ctgttatctg
     4921 tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga atgtggcctt
     4981 cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag ttgatgaaaa
     5041 cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc gattccccga
     5101 cctgtagctg aatgctatcc ggcgtcagcg caattttgct ttcctccacg gccagcaaaa
     5161 tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg tttacagcgt
     5221 caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca gataaagacc
     5281 ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg ttaccttcct
     5341 ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt acggtggtgg
     5401 tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag acctgttctc
     5461 tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac gttttggagc
     5521 ggatggtcat ctgcgtcttt gttcccggca ggctgccggg ggggcggttt tcctggtggt
     5581 aggtgcgccc cataatgatc ggctggtccg gatcgccgtt gaggaagtct acaatcactt
     5641 cctgacccac acgcggtatc gccaggtggc caaaaccggt gcctgcccac gcctgtgcca
     5701 cacggatcca gcacgaactg tcttggtctg ccgggttata acggtcccag ttgaacttca
     5761 cccgcacgcg accatgttcg tcgcagaaga tttcctcgcc tgccggtcct gtcacgacag
     5821 cgctctgcgg gccgtcgacc agcggtttca gcaacggctg tggtcgccat gttctgtctg
     5881 ccgggatcac cgcaaaatgg ttctccagcg ccgtccccgc tccctgccgt cctggcaccg
     5941 cctgtggctg ttcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg
     6001 cctgcggatg ccccgtcagc acaattcgtc gtcccggcca tatctccggc gagcgactca
     6061 ttccccgcgc ggtttctgca ttgtttcgcc agccgtccat ctgccagcgg gcaaagttct
     6121 gcccgtgagc acccttgaaa cgtccggggt agtcatacac ttcatactgc gtgcgctggt
     6181 agtcctggtg ctggccttcc tgttcaaaac gtccggccca gccggggcgt ttaaaggtgt
     6241 agtctttggt caccacggaa gaagggcgga tttgtgcgct gtagcggaac tggctgatgc
     6301 agagggtgct cacctcggta cgggtgttcg ggttccatgg gatttcaaaa gattcgggca
     6361 gatggcggac tgtgtcgcac agcaccaggc tctggtcggt acttttgtaa gcatgctcct
     6421 cataaaagaa gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcagtctcac
     6481 cgtactggac acaaaactca cgggaaggat gcggctcgct gaacagcgga ctccattcgg
     6541 tcaccccgtt ttcctgcaac atcgtgccga ggatgctttt gatgtcctcg ttctggaaga
     6601 tacggaagtt ctggcgcaga ccggcacgcc acagcggcgg gtgcaccttc atgctgtaca
     6661 gcatctggtt tttgtcgttc tccccgagtt caaaccaggt caccacgcct ttcacccggc
     6721 gctgtacttc atcgccctgc catatcgtca ggtaggccat tttgtccagc acctgcgcaa
     6781 attcaaggga gagaaactgc tggctgacca gggagagatc gagggaaaag agcgaagaga
     6841 gtgactggtt cagatgaaag gaaaccaccg caaaagcgtc cggcggcagg ccgtccactt
     6901 ccagtgtgaa acgtaatccg gttgacatag caacctccag taaaatgaat aatgatccgt
     6961 accgtacaac tgcggataat gtgataagcg ggacgacttc agacgttatt agggaaggaa
     7021 cagaaaatgc tatggttatg tgagaatttt gtttaaatat tactttacag aaacgggtgt
     7081 ctgttcagtc aaaaaaataa tcacaagctc aacctcttgt ttcttggcgt tattcaggac
     7141 aggtacgcat atcacaacga gtaacaaaaa cgcaaagtta aaccaatatt cataatatat
     7201 ttttattcac agcaataaat aatccaatta acattattaa aatgaatgat tgcataagaa
     7261 atat
//
LOCUS       DAZOYL010000201         7246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZOYL010000201 DAZOYL010000000
VERSION     DAZOYL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.202"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..440)
                     /gene="folK"
                     /locus_tag="ACHUOY_003010"
     CDS             complement(<1..440)
                     /gene="folK"
                     /locus_tag="ACHUOY_003010"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR0683732.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREV"
     gene            complement(437..1834)
                     /gene="pcnB"
                     /locus_tag="ACHUOY_003011"
     CDS             complement(437..1834)
                     /gene="pcnB"
                     /locus_tag="ACHUOY_003011"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR0683733.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1894..2790)
                     /gene="gluQRS"
                     /locus_tag="ACHUOY_003012"
     CDS             complement(1894..2790)
                     /gene="gluQRS"
                     /locus_tag="ACHUOY_003012"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR0683734.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2857..3312)
                     /gene="dksA"
                     /locus_tag="ACHUOY_003013"
     CDS             complement(2857..3312)
                     /gene="dksA"
                     /locus_tag="ACHUOY_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR0683735.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3490..4194)
                     /gene="sfsA"
                     /locus_tag="ACHUOY_003014"
     CDS             complement(3490..4194)
                     /gene="sfsA"
                     /locus_tag="ACHUOY_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR0683736.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4209..4739)
                     /gene="thpR"
                     /locus_tag="ACHUOY_003015"
     CDS             complement(4209..4739)
                     /gene="thpR"
                     /locus_tag="ACHUOY_003015"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR0683737.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4813..7242
                     /gene="hrpB"
                     /locus_tag="ACHUOY_003016"
     CDS             4813..7242
                     /gene="hrpB"
                     /locus_tag="ACHUOY_003016"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR0683738.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1648 a         1893 c         2034 g         1671 t
ORIGIN      
        1 acttcacgca acgtctcccc atcaggaaac gccagctccg gtgcaatctc aaacagcggc
       61 cacagcataa atccacgatt cttcatatcg tagtgcggaa cggtcaggcg ttcggtattt
      121 atcacttcat taccaaacag catgatatcg agatccagcg tgcgtggtcc ccagcgttca
      181 gctttacgaa cacgaccctg ttgcagttct atgcgctgtg tatggttgag taactcttcc
      241 ggggcgagcg aagtttcaag cgccacggcg gcgttgaggt aatcgggttg atcttgcggc
      301 cccagcggtg gagtgcggta aaacgaagaa acggcaagaa tgtggctttc agggatatcg
      361 cctaatgctt tcagggcagc attgacctgc tccagcggag aggccagatt gctgcctatg
      421 gcaatatacg ccactgtcat gcggtaccct cacgacgcgg tgcgcgtttg cgtggacgac
      481 gagtacggcg acgcggtgac ggctcttcat ccaactcgtt aagcatccct ttttgatctg
      541 gtggcgcgga aacctggaac tcaccccacc atttcaccag acgctgcagt tcagcgttac
      601 gttcaacttc agctcgcaag gccaacaggt cataagccgc acggaactta ggatgctcca
      661 gcagtttcca tgcgcgttta ccctgacgtc gggacatacg caactgcaac tgccagatat
      721 cgcgggttaa cgtcgtcagg cgtttcggga ttgccagtga acggcaggct tcgtccagca
      781 cgtcgttcat cgccagcgcg aaagcgtcgt gataggtcag gccgctttcc tgggcgatct
      841 tctgtgccgt ctccagcagt gggtaccaga acatggcggc aaacaagaac gccgggttca
      901 cgcgcatatc gttatggata cgcgtatcgg tattcttcag cacctgttca atgatccgct
      961 ccatcgggct gtcgccattt tccgtgaagt agcgggtaat ggtcgggaac agcggctgga
     1021 acagatgata ttcacacaac agcttatagg tttcgtaacc gtagcccgct tgtagcagtt
     1081 taagcgattc ttcaaacagg cgtgccggtg ggatatcgtt cagcagggta gcgaggcgag
     1141 ggatcggttc tgcggtttcc gggctgatgc gcatacccaa tttggcggca aaacgtaccg
     1201 cgcgcagcat ccgtaccgga tcttcacggt agcgcgtttc cgggttacca atcagacgga
     1261 taacgccgtc cttcagatcc ttcataccgc caacgtaatc acggacggta aaatccgcta
     1321 cgctgtaata gaggctgttg atagtgaaat cgcggcgctg ggcgtcttct tcgatggagc
     1381 cgaaaatgtt gtcgcgcagc aacatgccgt tttgcccgcg ttgggaggtc gtgcggtcgc
     1441 tgacgttacc ttcgtggtgt ccacggaagg tcgcaacttc gataatctcc gggccaaaca
     1501 ttacatgagc cagacggaaa cggcgaccca ccaggcggca gttacggaac agtttgcgca
     1561 cctgctcagg cgtggcgtta gtggttacgt caaaatcttt cggctttttg ccaagtaaca
     1621 ggtcgcgcac gccgccgcca accagccagg cttcgtatcc cgctttgttg agcctgtaca
     1681 ttaccttcag ggcattttca ctgatatctt tgcgggaaat agcatgctgc tcacgcggga
     1741 tcaccgtcac ctgtggacgg gcgactgcct gttcagcctc gctttcctcg cggcttagca
     1801 ccttgcggca aaaattagcg actcgggtaa aaatagtaca cctcggtagt gtcaaacatc
     1861 attcaggaca aaaaaatagc ggctaatcat agctcagcat gacgcatttg agaatgttga
     1921 atttacaatt gccgactcgg gcacggcggt cagcgtccag tttttgactg ctgactgaag
     1981 gatttgttcg acgctgaaat cctgccagtg tgcttctgcc tgctgaccca gaaattgaag
     2041 tgccgcgatt agtaccggac gtggatcgcc tttcggcaac gcaggcgcat gattctgctt
     2101 ggaaagttta gcgccttgtg gattaagcgc cagcggcaga tgaatgtaat ctggcacttt
     2161 ccagccaaaa agctggtaca gcgagatttg ccttactgtt ggttcaatca gatcagcccc
     2221 acgcactatt tctgtaacgc cctggaaatg atcatcaacc acaacagcca ggttgtaggc
     2281 gaacaaccca tcacggcgat gaatgataaa atcttcccgt gccagttttt cgtcggcgtg
     2341 aataatgccg cgcagctggt cagtaaattg cgtgaccgga tgctgctggc ggatacgcac
     2401 tgcggcattg tctggtccat gatgcaacac ccggcaatga ccgtcgtaaa taccgccaat
     2461 gctttgaata cgcgcacgcg tacaggtgca gtaataactt agtccttgtt catgtaacca
     2521 ggcgagtgct tcacgatagg cgtcgtgacg ttgcgattgc cagagaacat cgccgtccca
     2581 gtgcagaccg taatgttcca gctggcgcag gatagtttct gcggcaccgg gaacttcacg
     2641 aggcgggtcg atatcttcta tgcgtaccag ccagcgacct tgccgggcgc gagcctgcaa
     2701 atagctgccg agcgcggcga tcagagagcc aaaatgaagc tcgccggaag gagagggggc
     2761 gaagcggcca atatactgtg tgtctgtcat ctctttgaac aaaaaataag gcgggagcat
     2821 ttcccgcctg tggtaaacgt gatggaacgg ctgtaattag ccagccatct gtttttcgcg
     2881 aatttcagcc agcgttttgc agtcgatgca cagatcggct gtcgggcgcg cttccagacg
     2941 gcgaatacca atttcaacac cgcaggattc gcagtagccg aaatcttcgt cttccacttt
     3001 tttcagcgtc ttctcgatct ttttgatcag cttacgctcg cgatcgcggt tacgcagttc
     3061 gaggctgaac tcttcttcct gggctgcacg gtctaccggg tccgggaagt tggctgcttc
     3121 atcctgcata tgtgtaacgg tgcgatcgac ttcatccctg agttgattac gccatgcttc
     3181 cagaatacga cggaagtgcg ccagctgggc ttcattcata tactcttcgc ccggcttctc
     3241 ctgatatggt tccaccccag cgatggcgag aatactcagg gacgatgttt tacggttttg
     3301 cccttcttgc atgttgcttc tccttaacac gcactatcga tccccatgtt cgggggaaaa
     3361 atgaggccgc tataaatagc agatgctttt ccggatagca attatctaaa cgtaacactt
     3421 gacaactgtg tgaggaaaag cgtatttgcg cacgcgacca gaatgtaaat taaccagtta
     3481 cttactttac tacaatgtaa ccgacagtga ttttttaaga gccatgcctt cagcagaaat
     3541 ttccgctttg taagccagaa tttctacccc cctctgttga gcttctgaca atagttgcgc
     3601 gtatttctca tcgatgtggc gcgcgggtga aaaccgtgta atggctgaat gcagcacggc
     3661 gaaaaagata accgcacgct ggccttcagc cgctacgttc atcaactccc gaaggtgttt
     3721 ctgacctcgt tcagtgaccg catcgggaaa atatccctgt tcgttctccg ctaacgtaac
     3781 cgatttcact tcaatatagc agtctggacg cgaatccgcc tgcaacataa agtcaatacg
     3841 gctgcgttct gcgccgtatt ttacttcgct tttcagcgag ctatagcctg acagttctga
     3901 aattgattca ttaaggatag cctctttcgt caacctgtta gcccaaagcg tattgacgca
     3961 aataaatgcg ccgctctggc tttgagttag ttcccaggtg tgtgggtatt tccgtttggt
     4021 gttgtctgaa gtcgaatacc agacggtatc gccacgcgtt gcacaaccgg tcatcgcacc
     4081 cgtattcggg cagtgtagcg ttaattcgcg accatcgggt gtgatcacat cggctaaaaa
     4141 acgtttgtaa cgctgaatta gcgtcgcgcg ctgtagaggg ggagagaatt ccattgcgac
     4201 aatccttgtt attgcgttag cgcccagcgt tttagcggcg tgtagcgtgt gcgtccacgg
     4261 gcaaacgagg aggcgtaaag ggtgaactcc gtcaccgcat aagaccagtt aaaacctggc
     4321 ggcgggattg tcaccgcctc gctggcgtcg cgcaataagg taatatgtgg atgaaacgga
     4381 cgattgcttt gaaaacaacc gctgcgggca gcctgtgaac ggagcatatt cgccagctgg
     4441 attaagccgc gtggcggctg acgcatccct aaccacacca cacgcgaacg cagccattgt
     4501 ccggcgtcat caagcgtgag tgtgaaacca ggttgacgaa tccgtccggc taaaagagaa
     4561 agcgccttct ctttctcggc gctcacttcg cctaaaaatg ccagagtcag atgcaaatta
     4621 tcggcggcga ccggacgtcc cgcctcaggt gggaagtgtg tggcgcgcca gtggataatc
     4681 tgttcgcgga tttctgcagg taagtcgata gcaaagaaca gacgttgcgg ttcagacatg
     4741 tgaggcactc ggttatgaat taccgcgatg ctacaatgtg gcgcgaagaa tgttaaccct
     4801 ctggagcgtt ttgtgtcgtc gttgcccgtt gctgccgtct tacctgaatt acttaccgct
     4861 ctcgattgtg cgccgcaggt attattaagt gcgccgaccg gggccgggaa atcaacctgg
     4921 ctgccgctgc aactgctggc gcatcccggc attaacggga aaattatcct gctggagccg
     4981 cgtcgtctgg cggcacgtaa cgtcgcgcaa cggctggcgg agctgcttaa cgaaaagcca
     5041 ggcgataccg ttggctaccg gatgcgtgcg caaaactgcg tcgggccaaa tacccgtctg
     5101 gaagtagtta ccgaaggcgt gctgacgcgg atgatccagc gtgacccgga gttgagcggt
     5161 gttggattgg tgatccttga tgaatttcat gagcgcagct tgcaggcgga tttggcgttg
     5221 gcgctgttac tcgatgtgca acaaggtctg cgtgatgacc ttaaactgct gattatgtcg
     5281 gctacgctgg ataacgaccg cttgcagcaa atgctgccag aagcgcccgt cgtcatctca
     5341 gaagggcgct cgtttccggt tgaacgccat tatttaccgc tgcccgcgca tcagcgtttt
     5401 gacgaagccg ttgcggtagc caccgccgaa atgctgcgtc aggaaagcgg atcattactg
     5461 ttatttttac ctggcgtcgg agaaattcag cgtgtgcagg aacaactggc ttcgcgcatc
     5521 ggcagtgatg tgttgctctg cccgctgtat ggcgcgttgt cgctgaacga tcagcgaaaa
     5581 gcgatcctcc cggcaccgca agggatgcgc aaagtggtgc tggcgaccaa tattgctgaa
     5641 accagtttaa ccattgaagg tattcgtctg gtggtggatt gtgcccagga gcgtgtggcg
     5701 cgttttgagc cgcgcacggg gcttacgcga ctgattactc aacgcgttag ccaggcatcc
     5761 atgacgcagc gtgccgggcg cgccgggcgt ctggagccgg gtatctgcct gcatttaatc
     5821 gccaaagaac aagcagaacg cgctgccgcg caaagtgaac cggagatctt acaaagcgat
     5881 ctttccggtt tgctgatgga attactgcaa tggggatgca gcgatccggc gcagatgagc
     5941 tggctggatc aaccgccagt agtgaatcta atggccgcga aacgtctgtt acaaatgctg
     6001 ggggcgctgg acggtgaacg gcttagtgcg caagggcaaa aaatggcagc gctgggtaac
     6061 gatccgcgtt tagcggcaat gctggttagc gcgaagaacg acgacgaagc tgctaccgcg
     6121 gcaaaaattg ccgccattct cgaagagccg ccactgatgg gcaatagtga cctgggcgtg
     6181 gcgttttcgc gcaatcaacc agcctggcag caacgtagtc agcaactgtt aaaacgctta
     6241 aacgtacgcg gcggtgaggc agacagttcg cttatcgcgc cgctacttgc cggggcgttt
     6301 gccgatcgca ttgctcatcg ccgtgggcaa gatggacgct atcaactggc aaacggcatg
     6361 ggagcgatgc tcgatgccga cgacgcgcta agccgccacg aatggttgat cgcaccgtta
     6421 ttattgcagg gcagcgcctc gccggatgcg cggattttac tggcgctacc ggtcgatatt
     6481 gatgaattag tacaacgctg tccgcagctg gtgcagcaat ctgacaccgt ggaatgggat
     6541 gacgcgcaag gtacgctgaa agcctggcgt cggctacaaa tcggtcagtt gacggtgaaa
     6601 gtgcagccgc tggcgaaacc gtcagaagac gagttgcatc aggcgatgct taatggcatc
     6661 cgtgataaag gtttaagcgt gctcaactgg acggcggaag cggaacagct acgcttgcgt
     6721 ttgttatgcg ccgcaaagtg gttgccggaa tatgactggc cagcggttga tgatgaaagt
     6781 ttattggcag cgctggaaac gtggctgctg ccacatatga caggcgtaca ttcactacgc
     6841 ggcctgaaat cactggacat ttatcaggca ctacgcggat tacttgattg gggaatgcag
     6901 caacgtctgg atagtgaatt gcctgcgcat tacactgtgc cgacgggaag ccggatcgcc
     6961 attcgttatc atgaagataa cccgcccgca ctggcggtga gaatgcagga gatgtttggt
     7021 gaggccacta atccgactat cgcccagggg ggcgtgccgc tggtgctgga gttgctttca
     7081 cccgcccaaa ggccactaca aatcacacga gatttgagcg ccttctggaa aggagcgtac
     7141 cgtgaggtgc aaaaagagat gaaagggcgt tatcccaaac acgtctggcc ggacgacccg
     7201 gcaaacacgg caccgacgcg acggacgaaa aagtattcgt aaggtt
//
LOCUS       DAZOYL010000202         7233 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZOYL010000202 DAZOYL010000000
VERSION     DAZOYL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.203"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            112..723
                     /gene="ruvA"
                     /locus_tag="ACHUOY_003017"
     CDS             112..723
                     /gene="ruvA"
                     /locus_tag="ACHUOY_003017"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR0683739.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            732..1742
                     /gene="ruvB"
                     /locus_tag="ACHUOY_003018"
     CDS             732..1742
                     /gene="ruvB"
                     /locus_tag="ACHUOY_003018"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR0683740.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1889..2674)
                     /gene="znuB"
                     /locus_tag="ACHUOY_003019"
     CDS             complement(1889..2674)
                     /gene="znuB"
                     /locus_tag="ACHUOY_003019"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR0683741.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2671..3426)
                     /gene="znuC"
                     /locus_tag="ACHUOY_003020"
     CDS             complement(2671..3426)
                     /gene="znuC"
                     /locus_tag="ACHUOY_003020"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR0683742.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3505..4437
                     /gene="znuA"
                     /locus_tag="ACHUOY_003021"
     CDS             3505..4437
                     /gene="znuA"
                     /locus_tag="ACHUOY_003021"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR0683743.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4453..5775
                     /gene="mepM"
                     /locus_tag="ACHUOY_003022"
     CDS             4453..5775
                     /gene="mepM"
                     /locus_tag="ACHUOY_003022"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR0683744.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5895..6866
                     /gene="lpxM"
                     /locus_tag="ACHUOY_003023"
                     /gene_synonym="msbB"
     CDS             5895..6866
                     /gene="lpxM"
                     /locus_tag="ACHUOY_003023"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0683745.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6954..7233)
                     /locus_tag="ACHUOY_003024"
                     /pseudo
     CDS             complement(6954..7233)
                     /locus_tag="ACHUOY_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
BASE COUNT         1900 a         1771 c         1859 g         1703 t
ORIGIN      
        1 ttttcagttc atcgagacac ctcgcaagtt ttcttcatcc ttcgctggat atctatccag
       61 cattttttta tcatacagca ttatctttga ttcattacgc aggagcgtca tgtgataggc
      121 agactcagag gcatcatcat tgaaaaacaa cccccgctgg tgttaattga agtgggcggc
      181 gtaggctatg aagtgcatat gccgatgacc tgtttttatg aactccctga agcgggtcag
      241 gaagcgatcg ttttcaccca ctttgtggtg cgtgaagacg cgcaactgct gtacggtttt
      301 aacaataaac aagagcgcac gttgtttaaa gagttaatca aaaccaacgg cgtcggcccg
      361 aagttggcgc tggcgatcct ctccggaatg tcagcgcagc agttcgttaa tgccgttgaa
      421 cgtgaagaag tgggagcact ggtgaaactg ccgggcattg gtaaaaaaac cgccgagcgt
      481 ttaattgtcg aaatgaaaga ccgatttaaa ggtttgcacg gcgatctctt tacgccagcc
      541 gccgatctgg tgctcacgtc gcctgccagc ccggcaaccg acgatgctga acaggaagcg
      601 gttgccgcgc tggtggcgct gggctataaa ccacaagaag caagccgcat ggtgagcaaa
      661 atcgctcgca ctgacgccag cagtgaaaca ttaattcgcg aagccctacg cgccgcgtta
      721 tgaggtaaag gatgattgaa gcagaccgtc tgatttctgc cggtaccact ttgccggaag
      781 atggggcaga tcgcgccatt cgccctaaat tactggaaga gtatgttggt cagccgcagg
      841 ttcgttcaca gatggagatt ttcatcaaag cagcgaaact gcgcggcgat gccctcgatc
      901 atctgttgat ttttggtcct ccggggttgg gtaaaactac gcttgccaac attgtcgcca
      961 atgaaatggg cgttaattta cgcacgactt ctggtccggt gctggaaaag gcgggcgatc
     1021 tggcagcgat gctcactaac cttgaaccgc atgacgtgct gtttattgat gagatccacc
     1081 gtctatcgcc agttgttgaa gaagtgctgt acccggcaat ggaagactac caactggata
     1141 tcatgattgg tgaaggtccg gcggcacgct ccattaaaat tgatttgccg ccgtttaccc
     1201 tgattggtgc aaccacgcgc gcaggttcgc tgacatcacc gttgcgcgac cgttttggta
     1261 ttgtgcaacg tctggagttt tatcaggtgc cggatctgca atatatcgtc agtcgcagcg
     1321 cacgctttat ggggcttgag atgagtgatg acggcgcgct ggaagttgct cgtcgcgctc
     1381 gcggtacgcc acgtattgcc aaccgtctgc tgcgtcgagt gcgtgatttc gccgaagtga
     1441 agcacgatgg caccatctcg gcagatatcg ctgctcaggc gctggatatg ttgaatgtcg
     1501 atgctgaagg tttcgattat atggaccgca aattgttgct ggcggtaatc gataagttct
     1561 ttggtgggcc ggtaggtctg gataacctgg cggcagccat tggcgaagaa cgtgaaacca
     1621 ttgaggatgt gctggagcct tatctgattc agcaaggctt tttgcagcgt acaccgcgtg
     1681 ggcgtatggc gacgacgcgg gcgtggaatc actttggcat aacgccgcca gaaatgccgt
     1741 aagtcggatt ggtcgctcgc accgcatccg acattggcag tgtgcctgat gcgacgctat
     1801 tcgcgtctta tccggcctac cagagatagc gaaccgtagg tcggataagg cgctcgcgcc
     1861 gcatccgaca aatgtgttca gcgacagatt agctggcctg ctttttcatc atactgataa
     1921 taaataacag tgcagcacat agcactaccg acggacccgc cggcgtatcg taaaacgcgg
     1981 aaaaggttaa accgccagtt actgccacca tccccaccaa aacagcgaca ccagccatct
     2041 gttccggcgt acgggcaaag cgacgcgcag tagctgcagg aataatcagc aacgaagtaa
     2101 taatcaacgc accgacaaat ttcatcgcta caccaatcgt caatgccgtc accagcatca
     2161 acaacaattt cacgcgctgt aatttcacac catcaacaaa cgccagatcc gggctaatcg
     2221 tcatcgacag caaattgcgc cattgccaga acaaaatagc caccacgatg accacgccaa
     2281 tcgcaataga gatgagatct tctggcgtca ctgccagcaa atcaccgaac aggtaagcca
     2341 tcaaatcaac acgaatatta gacatcagac taacgaccac caggcccagc gacagggcac
     2401 tgtgcgccat aatccctaat aacgtgtcga tcgccagctg tggacgcttc tccagccata
     2461 ccagaccgcc cgccagcagc agcgtaacgg caatcaccgc atagaatgga ttcacgtcca
     2521 gcaacaaacc aaacgcgaca ccaagtaatg aggcatgagc cagcgtatca ccgaaataag
     2581 acatacgacg ccagactaca aacgaaccca gcggacccgc ggcacaggcg agcatgatcc
     2641 cggctaacca accgggaaat aataattcaa tcatgagcga tcatttcccc gacgcaaaac
     2701 aattcgtccc tgtaaatcgt gacgatggtt atgatgatgg cgatagatac ccagttgttc
     2761 agcaccacga ggaccaaaca ttgaaataaa ctccggatgc agggaaacaa cttccggtgt
     2821 gccggaacaa caaatgtggt gattcaggca cagcacttca tcggtttttg ccatcaccag
     2881 atgcagatcg tgagaaacca ttaaaacgcc acaatccagt tcacgacgca gttggtcaat
     2941 aaggtcatat aacgccacct ggccatttac atccacgcct tgagtgggtt catccagcac
     3001 taataattgc gggcgattta acaatgctcg cgctaacagt acacgctgcg tttcgccacc
     3061 agagagcttt tgcatcggtg cgttaatcag gtgcccggcc tggacacgtt tcagtgcagg
     3121 caaaatatct tctttatgtg taccagggcg taagcgtaaa aaacggttta cggtcagtgg
     3181 caacgtggtg tcgagataca gcttctgcgg tacatagccg atgcgcagtt ttccgttgcg
     3241 cttgataacc ccttcatcgg gtgttaccag cccgagcact acccgtacca gtgtcgactt
     3301 acctgcgcca tttggcccaa gtaaagtcaa aatttttcca ggtttaagtt ccagcgacac
     3361 atcagagagg acgcggcgtt ggccaaaaga aaccgagaca ttttccaagg aaaccagact
     3421 tgtcatgtta attttagtct tgcagtagtc atgaaatgtt ataatatcac acttctcata
     3481 ttcattacga tgattggtcg cattatgtta cataaaaaaa cgcttctttt cgcagcatta
     3541 tccgccgctc tctggggagg tgcaacacag gccgcagatg ccgccgttgt cgcttcgctt
     3601 aaacccgttg ggttcatcgc ttctgccatt gctgatgggg taacagaaac agaggtttta
     3661 cttcctgacg gcgcttcaga acatgattat tcactgcgcc catcggatgt aaaacgctta
     3721 cagaacgcgg acttagtcgt ttgggttggc ccggagatgg aagcgtttat gcaaaaaccg
     3781 gtaagcaaat taccaggagc gaagcaggta acgattgcgc agcttgaaga tgtgaaaccg
     3841 ctactgatga aaagtattca cggcgatgat gatgatcacg accacgcgga aaaaagtgac
     3901 gaagatcacc atcacggcga tttcaacatg catctttggc tttccccaga gatagcgcgg
     3961 gctacagcgg ttgcaatcca tggaaaatta gtggaactta tgccgcaaag tcgagccaaa
     4021 cttgacgcca acctgaagga ttttgaggca caattagcct caaccgaaac gcaggttggt
     4081 aacgagctcg cgccgctcaa ggggaaaggt tatttcgttt ttcacgatgc ttacggctat
     4141 ttcgaaaaac agttcggact gacaccgctt ggtcatttta ccgttaaccc tgagattcaa
     4201 cctggcgcgc agcgtttaca tgaaataaga acacagttgg ttgagcaaaa agcaacctgc
     4261 gtttttgctg agccacagtt caggccagcg gtcgttgaga gcgtcgcacg agggacatcc
     4321 gttcgtatgg gaacgttgga tcccctgggg acgaatatca aactgggtaa aacaagctat
     4381 tcagaattcc tgagtcaatt agccaaccag tatgcgagct gcctgaaagg agattaatga
     4441 ggaagtgatt acgtgcaaca gatagcccgc tctgtcgccc tggcgtttaa taatttaccg
     4501 cgaccacacc gcgttatgtt ggggtcgctc accgttctta ctctggccgt cgctgtctgg
     4561 cggccctatg tttatcaccg cgatgccacg ccaattgtca aaaccattga gctggaacag
     4621 aacgaaattc gttcgctctt acctgaagcc agtgagccga ttgatcaagc tgcacaagaa
     4681 gatgaagcca ttccccagga cgaactggat gacaaaatcg ccggtgaagc gggcgtgcat
     4741 gaatatgttg tttccactgg cgatacgcta agcagcattc tcaatcagta tggtattgat
     4801 atgggtgata tcacccaact ggctgcggcc gacaaagaat tgcgtaacct gaaaatcggt
     4861 caacaactct cctggacatt aaccgcggac ggcgaactgc agcgcctcac ctgggaagtg
     4921 tctcgtcgtg aaacccgaac ctatgaccgt actgccgcta acggttttaa aatgaccagc
     4981 gaaatgcagc aaggagagtg ggttaacaat ctgctgaaag gtaccgtcgg ggggagcttt
     5041 gttgccagcg ccagaaacgc cggtttaacc agcgccgaag tgagcgcagt gattaaagcc
     5101 atgcagtggc aaatggattt ccgcaaactg aaaaaaggcg atgaatttgc ggtgttaatg
     5161 tctcgagaaa tgcttgatgg taaacgtgag caaagccagc tgctgggcgt acgtttgcgt
     5221 tcagaaggta aagattatta cgctatccgc gctgaagatg gcaaattcta cgatcgtaac
     5281 ggtactggtc tggcgaaagg attcttgcga ttcccgacgg cgaaacagtt ccgtatctcg
     5341 tctaacttta acccgcgtcg tactaatccg gtgaccggtc gcgttgcagc acacagaggt
     5401 gttgatttcg ccatgccgca gggtacgcca gtgctttcag tgggtgacgg tgaagtggtg
     5461 gttgccaaac gcagtggcgc agcaggttat tatgtggcta ttcgtcatgg tcgcagctac
     5521 accacgcgtt atatgcactt gcgcaagatt ctggtgaaac cgggacagaa ggtgaaacgt
     5581 ggcgaccgta tcgcgctttc cggtaatacc ggacgttcaa ccgggccgca tctgcactat
     5641 gaagtatgga taaaccagca ggccgtaaac ccgctgacgg caaaactgcc gcgtaccgaa
     5701 gggctgaccg gctccgatcg tcgcgaattc ctggcgcagg ccaaagagat tgtgccgcag
     5761 ctacggtttg attaattaac atccattcgc agccggtacg cagtcagtat cggctttttt
     5821 tatttggtgc ggggcaagtt gcgccgctat actatcacca gattgatttt tgccttatcc
     5881 gaaactggaa aagcatggaa acgaaaaaaa ataatagcga atacattcct gagtttgata
     5941 aatcctttcg ccacccgcgc tactggggag catggctggg tgtagcagcg atggcgggta
     6001 tcgctttaac gccgccaaag ttccgtgatc ccattctggc acggctggga cgttttgccg
     6061 gacgactggg aaaaagctca cgccgtcgtg cgttaatcaa tctgtcgctc tgctttccag
     6121 aacgtagtga agctgaacgc gaagcgattg ttgatgagat gtttgccacc gcgccgcaag
     6181 cgatggcaat gatggctgag ttggcaatac gcggaccgga gaaaattcag ccgcgcgttg
     6241 actggcaagg gctggagatc atcgaagaga tgaggcgtaa taacgagaaa gttatctttc
     6301 tggtgccgca cggttgggcc gtcgatattc ctgccatgct gatggcctcg caagggcaga
     6361 aaatggcagc gatgttccat aatcagggca acccggtttt tgattatgtc tggaacacgg
     6421 tgcgtcgtcg ctttggcggt cgtctgcatg cgagaaatga cggtattaaa ccattcatcc
     6481 agtcggtacg tcaggggtac tggggatatt atttacccga tcaggatcat ggcccagagc
     6541 acagcgaatt tgtggatttc tttgccacct ataaagcgac gttgcccgcg attggtcgtt
     6601 tgatgaaagt gtgccgtgcg cgcgttgttc cgctgtttcc gatttatgat ggcaagacgc
     6661 accgtctgac gattcaggtg cgcccaccga tggatgatct gttagaggcg gatgatcata
     6721 cgattgcgcg gcggatgaat gaagaagtcg agatttttgt tggtccgcga ccagaacaat
     6781 acacctggat attaaaattg ctgaaaactc gcaaaccggg cgaaatccaa ccgtataagc
     6841 gtaaagatct ttatcctatc aaataaaaaa agcctctcaa attattgaga ggctgtactc
     6901 gataaaaaat ctcaatgagt gttcccacta ttttgagttt ttaggttagt acgctattga
     6961 gttaatcgta gcgtgataat ttcaaaaggt ttgaactcaa gattgcaata ttgattgcaa
     7021 taaaaactgt catctaactt attttcttga agatcgacat attgaacgta gtgataatta
     7081 catactcacg aaagcatggc tgaaatttct aatccatgac tctcatataa tcttacgatt
     7141 ataccgtcgc cgtcttcagc taattttatt gtttcgatga taacgctgtt agtatcgata
     7201 gcaaaaaatg atagtgaagg gggtaattca ccc
//
LOCUS       DAZOYL010000203         7121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZOYL010000203 DAZOYL010000000
VERSION     DAZOYL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.204"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..435
                     /locus_tag="ACHUOY_003025"
     CDS             <1..435
                     /locus_tag="ACHUOY_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR0683746.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="ACHUOY_003026"
     CDS             518..1420
                     /locus_tag="ACHUOY_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR0683747.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHUOY_003027"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHUOY_003027"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR0683748.1"
                     /translation="MDVSYLLDSLNDKQRETVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHUOY_003028"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHUOY_003028"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR0683749.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHUOY_003029"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHUOY_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR0683750.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="ACHUOY_003030"
     CDS             complement(5330..6037)
                     /locus_tag="ACHUOY_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR0683751.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHUOY_003031"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHUOY_003031"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR0683752.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="ACHUOY_003032"
     CDS             complement(6895..7098)
                     /locus_tag="ACHUOY_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0683753.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1640 a         1990 c         1853 g         1638 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgtt tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAZOYL010000204         7062 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZOYL010000204 DAZOYL010000000
VERSION     DAZOYL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.205"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..302
                     /locus_tag="ACHUOY_003033"
     CDS             <1..302
                     /locus_tag="ACHUOY_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0683754.1"
                     /translation="QRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVP
                     SSAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(391..903)
                     /locus_tag="ACHUOY_003034"
     CDS             complement(391..903)
                     /locus_tag="ACHUOY_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR0683755.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1098..1862
                     /locus_tag="ACHUOY_003035"
     CDS             1098..1862
                     /locus_tag="ACHUOY_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683756.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1813..3216
                     /locus_tag="ACHUOY_003036"
     CDS             1813..3216
                     /locus_tag="ACHUOY_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR0683757.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3216..4682
                     /locus_tag="ACHUOY_003037"
                     /pseudo
     CDS             3216..4682
                     /locus_tag="ACHUOY_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4573..5316
                     /locus_tag="ACHUOY_003038"
     CDS             4573..5316
                     /locus_tag="ACHUOY_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR0683758.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5320..6540
                     /locus_tag="ACHUOY_003039"
     CDS             5320..6540
                     /locus_tag="ACHUOY_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR0683759.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6544..7050
                     /locus_tag="ACHUOY_003040"
     CDS             6544..7050
                     /locus_tag="ACHUOY_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683760.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1873 a         1685 c         1934 g         1570 t
ORIGIN      
        1 gccagcgtga tgctgatgca ctcgatgcta aatacacgaa ggagttagct gatgcgaaag
       61 ctgaaaatga tgctcttcgg cgcaagcttg ataatggtgg tcgggtgctc gtcaaaggaa
      121 aatgccctgt gccatcctca gccgaaacct ccagcgcctc cggcatgggc aatgatgcca
      181 ccgtcgaact ctctccagtt gctggacgaa acgttctcgg tatccgggac ggaatcatca
      241 gcgaccagac agcattgaga atgcttcagg agtacatcag gatccaatgc cttggggggt
      301 agcggtaatt ttactcatta tccttcaaat cagattctgt tgtcagagga atgggggaag
      361 ctggattcgc aagagctttt tatgggcagt ttagctaaaa aaaggcaaca cgaagctaaa
      421 actaccaaca taaagtgctg accagctagc agctgaaatc agactcactg tatacacttt
      481 cactggatga agttgcaaca ttcctgccac caaaggagca atataacgca gcacggcgat
      541 aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc aactgaaggc gaacccgttc
      601 tattgtattg gaacggcttg atattataca tgcaagttga gggatatgcc caattattat
      661 accaagatgg taattaacga tagtaccgca ccatgcgcca ctcatgaccg tgattccagc
      721 ttcccatggt gaaagagtcg tcttgctgac tgtaattaca gtggccatca taattgatgc
      781 tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg aacgctatta taaaaagaag
      841 cccccatagg cgatatggat gaagtacaaa atagttcatc aaagcattta tccactccat
      901 caatgtcttt ccttctgtga atttttcttt gctatttaac cgtgatacga cttaatttag
      961 gcttaaacac cttacattta caaggtactc ctgtgggggg ccctgccacg gagcgtcgca
     1021 ggagtgagtg atgaggaaga attaaagcaa cctattgtga caggcagcga taacagtaaa
     1081 tcacaggaag cataatcatg gcaaaaccgg actgggaggc catcgaatcg gcataccggg
     1141 ccggagtcct tagcctccgt gatataggcg agaaatacgg cgttacagaa ggggctatca
     1201 ggaagagggc caaaaagctt ggttgggcac gcagtggcgg tacgcaggtt tgcaaaaatg
     1261 gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc cattactggc cttacacaaa
     1321 aaagtacgca actaaaaaca gaatctacac cggatacgaa accgatacgc ggaatgcgta
     1381 ccgatccccc gactaaccca ttccaacccg gtaaccagca ggcattaaaa cacggtggtt
     1441 atgcccgtcg ccttctgctc aaagatgagg tgatagagga cgctaaagcg ttgacgctcg
     1501 aggacgaatt atttcgcctt cgtgctaaca accttgtcgc tgcagagaat attggtcggt
     1561 ggttggtgtc gctggaagat gctaatgggg accaggaaag gaagatgctg attgaaaata
     1621 tcagcgccgc cgagaaagca atgatgcgca atacagttcg tattgagtcc atcgttggca
     1681 cgcttgcgac ggtaggaaaa atatttgctg atacagccta ccgcaaggcc gccactgata
     1741 aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg tattgatgat ggcaatggag
     1801 agcgtgacct caatgacttc tactctgaca tccaaaccga cgctgaatcc ggctttacgt
     1861 agtttctgga ctatgcgggc acgtaacaaa gtgctttatg gtggtcggtc atcgtcaaaa
     1921 tcatgggatg ccgctggcat tgccatattt ctgtcgaata aatacaccct gcgtttttgt
     1981 tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt ataccctgct caaaattcag
     2041 atagacaggt ttggtctgcg gcaccgtttc cgtattctga acaacaaaat cattaaccgg
     2101 gttactggct cggaatttgt tttttatgga ttatggcgca acatcgaaga aattaagtca
     2161 ctggagggga tcgatgtgtt gtggctggaa gaagcccacg cactgacgga ataccagtgg
     2221 aaaattctgg agccaacgat ccgtaaagag ggttcggaat gctggttcat attcaacccc
     2281 ggacttgtta ctgatttcgt ctggcgcaac ttcgttgttg atccgcccga aggcactctc
     2341 atccgcaaaa ttaactatga cgaaaatccg tttctgtctg acaccatgct taaggttatc
     2401 gacgcggcgc gacgccgtga tccggatggt tttaaacatg tgtatgaggg cgttccggag
     2461 tctgatgatg atgcggcaat catcaaactg tcttggatag aagccgcagt ggatgcgcac
     2521 aaaacgttaa atttcgaacc cagtggaaga aagcgtattg gctttgacgt ggctgacagt
     2581 ggtacagata agtgcgctaa cgtttaccgt cacggatccg ttgttttctg ggccgacgaa
     2641 tggaaggcca aagaagatga attactgaag agctgccagc gtacttatca ggcggcgctg
     2701 gagcgtgaag cagatattgt ttacgactct atcggtgttg gtgcgtctgc cggtgctaaa
     2761 ttctctgaaa ttaacgctga ccggaagagc gagaacgcat acgcgcgacg tgtgaattac
     2821 cagaggttta acgccggtgc tggtgtgcat gagccagatg acgagtacaa cggcatcccc
     2881 aacaaagact ttttcgcaaa tcttaaggct caggcatggt ggctggtggc tgaccgtttc
     2941 agaaatacgt ttaacgccat taacaacgga gaacagtatc ctgtggatga gctgatcagc
     3001 atagattctc gttgtccgtt gcttgaaaag ctgaaactgg aactgacaac acctcatcgt
     3061 gatttcgacc gtaacggacg tgtgatggtc gaaagtaaaa aagacctcgc aaaacgcgag
     3121 ataccgtcac caaacgttgc tgatgcattc attatggcct tcgcgccaat cgatacatcg
     3181 ctggatatct gggaacagct ggggagacag gcctgatggc acgaaacaaa caagccctgc
     3241 ggcgaactgt gcaggccaca gctgatggtt atgagaattt tattgcccgc gtagggatgc
     3301 agacacctaa ccagcactca gcatccacct accgggctaa tttcaccagt cgtaaccgca
     3361 tgctggtgga atggtcctat cgttcatcct agatcatcgg cgaagcagtc gatgctatcc
     3421 cggatgatat gacccgcaaa ggcattcgca tcacttcgga aattgatgca aaagatcgtg
     3481 gcattctcga atcacaactg gatgagttgc aaatctggga tgcgctgaat gacgtgctga
     3541 aatggtcgcg cctctacggc ggcgcggtgg gtttcatcat gattgagggg taggcaccaa
     3601 tgaccccgct gcgacccgaa accatcggta agggcaagtt taaggggatt ctcccgctcg
     3661 accgctggat ggtcgacccg gcactgaccc gccgcattaa agatatgggg ccggacctgg
     3721 gtaaacctga gttttacgat gtggtgacca cagcaacggg aattcctgcc tggcgcattc
     3781 atcacagtcg cctgattcgc tttgatggcg tcacgttgcc atttcagcag aagatgaccg
     3841 agaacgaatg gggaatgtcg gttgtagagc gtatctggga tcgtcttacc gcgttcgaca
     3901 gcgctactgt cggcgcggcg cagctggtct acaaggcgca tttgcgtacc tacagcgtgg
     3961 agaagctacg cgagcttatc gcacttggtg gtcctgcgta tgaagcgttg ctgaagaata
     4021 tcgacctgat tcgacagttc cagagtaatg aaggcatgac gctcatggac tcgcgggata
     4081 agtttgaaac ccatcagtac agcttcagtg gtctggatga catcctttcg cagtttgtag
     4141 aacagattag tggcgctgtt ggtatcccac tggtgcggtt gttcggacag tccccgaaag
     4201 gattttctac cggtgatgca gaccttgcca actattacga tcgcatcagt tcgttgcagg
     4261 agaggcgttt acgtcttccg gtgcggcgga tactggacat catgcatcgt tcggaacttg
     4321 gcaagccgct gccggacgat ttcacgtttg agtttaaccc gctctggcaa atgtctgatg
     4381 tcgatcgctc aacggtggcg ttaaacacca ccaacgcaat cagtacggcg ctgggtgatg
     4441 gtctgatgac actgaaagcc gctatgactg atttgcgaga aaattctgac gtaaccggca
     4501 tcggggcatc cattaccgac gaggacatcg agaatgccga agatgaagcg ccgcccggca
     4561 tcggcgaacc tgatgacgaa ccgcaggagc cgtcaggcgg aaatccggta tcgaaccagc
     4621 ctacgcagga tagcgagggc ggtcggagac atcgtaaatg gtcgctacga tggttcaaat
     4681 gacagtatca cggaaattat tgcggcgctg gagcgttaca gtgaaatcat caccccctgg
     4741 gcgacaaagg tcgcggaaaa ctttactgcg gacctaaccc ggcagaacga gaaagtttgg
     4801 cggcaacaca gcaagaacat cagtcgcgag ctccgcaatc ttgtggaaag cgctcctgtg
     4861 ggccaggtga tgcaatccat catcgccgaa caggtcaagt acatcaaatc tctgcctctc
     4921 gaggccgcag acagggtgta cgacatccag aacaaagcga tagaggctgt tgtcaccggt
     4981 ggtcgggcgg agcagtttgc taaagagatt gcatctaccg gcgatgttgc taagtctagg
     5041 gccgatctga ttgcccgaac ggaactggga agagcaacgg gcgcgctgga tatggcccga
     5101 gcgatagcta ttggctcgga tggttatatt tggcgtacag ccgatgatgg cgatgtccga
     5161 gattcccacg atcacatgaa aggtaaattc gtccgctggg attcacctcc aactttggac
     5221 ggcatgaccg gccacgcggg cgagctgcca aactgccgct gctataaaga gatcgtgttt
     5281 gttcgcgttc cattcgcaat gaaaagggca gcataaccca tgaaatactt ttttgagacc
     5341 aggctcgggg aaacccgata ccgcctggct gacggctcgt tgctgtgcaa agacgtgccg
     5401 ataggacgaa caggtaagca gctctatggt gctgatgacc tgccaaaact gaaacccgat
     5461 aagttcggtg aaatagtcgt cacgcgttct cctgagcagg tattccatcc ggccacgctt
     5521 gcctcattcg aagggatgag catcacgatc ctgcatcctg aagatgaaaa cgggaatgtg
     5581 cggctggtaa atcccgagaa ctggaaagag cttgctgtcg ggcacctcca gaatgtccgg
     5641 cgcgggacgg gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa agacgaaagc
     5701 gccattcagc ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga cgcggagtac
     5761 gagcagaccg agccaggtaa agccgagcag gtcgatatta ccggaaacca tgtggctctt
     5821 gtccctaaag gcagagccgg aaatcgttgt gcaattggag acagagacac aatggcaaat
     5881 caaaagaaaa actggtggaa ccgcatgcgt gcggccatca agacaggaga tgccgacacc
     5941 atgaacgaac tggtggagtc ggctcccgca tcggttacag gagatgaggg ggatttgccg
     6001 cagggcgtta atctcaacat caacctgtcc ccgcagcaac cactaccgga caaagcacca
     6061 gagatgggcg gagatccaac cggcgacagt gatgatgacc tcaaaacatt actgaaagcc
     6121 ctgctggcta agctggaaag aaatgccacg ggcgataacg ataataagcc tgacgataat
     6181 ccgaccggtg acggcgagga cgatgaagag gaaaccacga ttactggtga ctcagcctgg
     6241 cgtgccgaag ttatcgttcc gggtatcgat ctgagccgta agatgaaacc gaccgcgttc
     6301 aaacgcgagg ttctggcttc tgctgacaaa acgctggttc gccagatagt cggtgatgcg
     6361 gatatccgca aattaccgaa acaatcggtc gacatggcgt ttaatgccgt gtctgaggtt
     6421 gccaaagggc gaaacacccg cgccaccacc ggcgatgcac agcgcctaaa catgggcatg
     6481 accagtatcg cttccctgaa caaacaaaac gctgaattct gggcaaaccg taaagggtaa
     6541 aaaatgaata atgtatttct gtaccggatg cctgttggca ttgccggggc tgtctctcgc
     6601 ccgcaggact taaccgtcga accggtggtc cttaaatccg ataacgcctt cgctgcctat
     6661 ggcctggctg gtaaatacga tgatgacggt tttttcgtgc cgctggcaga tggtgatacc
     6721 gcagacaagg tgaaggggat ctacgtgcgc ccttatccga ccacgtcgca gccggacatg
     6781 gttcgccagg tgggaacagg caagaacttc ccgggcgacg ccatgaagcg tggctacgtg
     6841 accgttaatc tcggttctga ttttgatgcc agcactatca aaaaaggcga tccggtatac
     6901 gttgtcgtct ccactgatga atccatcaaa gtgccgctgg gtggattcat gtccacgtca
     6961 gtcagtggca aaaacgtggt gctgaccaac gctgaattca caggtgccgg tgatgctgac
     7021 ggcaatgcag aaatttcctg gaagatttaa ggaacagacg aa
//
LOCUS       DAZOYL010000205         7057 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZOYL010000205 DAZOYL010000000
VERSION     DAZOYL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.206"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            337..1374
                     /gene="dusA"
                     /locus_tag="ACHUOY_003041"
     CDS             337..1374
                     /gene="dusA"
                     /locus_tag="ACHUOY_003041"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR0683761.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1508..1750
                     /gene="pspG"
                     /locus_tag="ACHUOY_003042"
     CDS             1508..1750
                     /gene="pspG"
                     /locus_tag="ACHUOY_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR0683762.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1916..2899)
                     /locus_tag="ACHUOY_003043"
     CDS             complement(1916..2899)
                     /locus_tag="ACHUOY_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR0683763.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            2982..4397
                     /gene="dnaB"
                     /locus_tag="ACHUOY_003044"
     CDS             2982..4397
                     /gene="dnaB"
                     /locus_tag="ACHUOY_003044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0683764.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4450..5529
                     /gene="alr"
                     /locus_tag="ACHUOY_003045"
     CDS             4450..5529
                     /gene="alr"
                     /locus_tag="ACHUOY_003045"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR0683765.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5782..6975
                     /gene="tyrB"
                     /locus_tag="ACHUOY_003046"
     CDS             5782..6975
                     /gene="tyrB"
                     /locus_tag="ACHUOY_003046"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR0683766.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1660 a         1747 c         1879 g         1771 t
ORIGIN      
        1 aggcattata taaaaggacc caatatttat tgggttcttt tttctctatc aatgctatta
       61 gcagggagat atatcaccag agtttaatgt gtgatttttt atttatcgtc gaacctggat
      121 tgtttatcat tggccttaac aaagttaacg gctaataaga ttatttccat cacttcgtga
      181 gagcttcatg ccttgagagg atctcaattt tcttttgcaa tgagacaggc gcttcctgtt
      241 gttatggtat agtaccccgc tattgagcct cctgaatagt gatgctgaat aacataaccc
      301 catgatatat cgataaaata atctctacat ttgaaaatgc acggtaattc tgaaatgcaa
      361 aaaatcaacc aaaccagcgc aatgcctgaa aaaactgacg ttcactggag tggtcggttt
      421 agcgttgcac caatgctcga ctggacggac agacattgcc gctatttctt gcgtctgctt
      481 tcccgcaata cgttgctgta taccgaaatg gtgaccacag gggcgattat tcacggtaaa
      541 ggtgattacc tggcgtacag tgaagaagaa catccggtag cgttgcaact cggcggtagc
      601 gatccggcgg cgctggcaca gtgtgcgaag ctggcagaag cgcgtggata tgatgagatc
      661 aacctgaatg tcggctgccc gtctgaccgg gtgcagaacg gcatgtttgg tgcgtgtctg
      721 atgggtaatg cgcagctggt tgccgactgc gtgaaagcga tgcgcgatgt ggtgtcgatt
      781 ccagtgacgg tgaaaacgcg tattggcatc gatgaccagg acagctatga atttctctgc
      841 gatttcatca acaccgtttc cggcaaaggc gagtgtgaga tgtttatcat ccacgcacgt
      901 aaagcctggc tttcggggtt aagcccgaaa gaaaaccgtg aaatcccgcc gctcgattat
      961 ccgcgtgtgt atcaactgaa gcgtgacttt ccgcatctga caatgtcgat taacggtggt
     1021 atcaagtcgc tggaagaggc caaagcacac ctgcaacata tggatggcgt gatggtcggg
     1081 cgcgaggcgt atcagaatcc gggtattctg gcggcggtag accgggagat ctttggttcc
     1141 tcggataccg atgccgatcc ggtggcggta gtgcgcgcca tgtatccgta cattgagcgt
     1201 gaactcagcc aggggacgta tctcggccat attacccggc atatgttggg cttgttccag
     1261 ggtattcctg gcgcgcggca gtggcggcgt tatttaagtg aaaatgccca taaagcgggt
     1321 gcagacatta atgtgctgga acacgcgctc aaactggtgg cggataagcg ttaacttttc
     1381 accaaaaagt agtcaaattc accacgccct gcgcaccgtc gcggggcgtt ttgctgttaa
     1441 atcaatagat tatttttggc atgattcttg taatgccagc aagagatttc atatttggga
     1501 gagcatcatg ctggaactac tttttgtgat tggctttttt gtcatgctga tggtcaccgg
     1561 cgtttcgttg ctgggcatta tcgccgcgct ggttgtggcg acggccatta tgttcctcgg
     1621 cggtatgctg gcattgatga ttaagttgct gccgtggtta ctactggcga ttgcggtggt
     1681 gtgggttatt aaggcgatta aagcaccaaa agtgccgaaa tatcagcgtt atgaccgctg
     1741 gcgttactaa gggattgtgc ggatgatcac aacctaaggt tttatcctta gaacaaaata
     1801 ggaattgata atcaaatctg tcactattgc gcctctaaca gattcatcgt gctgtaccct
     1861 acatacagcc gaactataaa aagaaagggc ttcccaggtg gaagccctat ttcttttatg
     1921 gaatcaacag gctggaacct tgcgtcgccc ggctttccag aatctcatgc gcacgctgcg
     1981 catccttcag cggatatttc tgctgctcgg cgacatcgac cttaatcaca ccgctggcaa
     2041 tcaaagagaa cagttcatta ctggcctcgg ttaattcctc ccgcgtggtg atatagcctt
     2101 gcagggaagg gcgtgtcaca tacaacgagc ctttttgatt gagaatgcct aagttcacac
     2161 cggtaaccgc acctgatgag ttgccaaaac tgaccattaa gccgcggcgt tgcaggcaat
     2221 ccagcgaccg ttcccaggtg tctctgccca cggaatcgta caccacgcgc actttcttac
     2281 cgccggtgat ctcttttaac cgctcgacca gatcctcttc acgatagtta ataacctgcc
     2341 acgcgcccgc ttttagcgcg ctctgcgctt tttgcgcggt tcctacggtg ccgataagtt
     2401 tcgcgcccag ggcttttgcc cactggcagg caattaagcc aacgccgcca gccgctgcgt
     2461 ggaacaggaa ctgttcatcg ggtttaattt cataggtttt gcgcagcaga taataaaccg
     2521 ttaagccttt caggaaggat gccgcagctt gctcaaaaga aattgccgca ggcagaatcg
     2581 ccgctttatc cgcattaatg ttatgcacag agctgtaagc gcctaacgcc gactgcgcat
     2641 agactacacg atcgcctgcc ttaatatgct ttacaccact gccgacttta ctcacgatgc
     2701 ctgccgcttc ggtacctaat ccgctgggta gtgatggcgg cgggtaaagg ccgctgcgga
     2761 tatacgtgtc gataaaattg atgccgatgg ctttattttc gacctggatt tcattctctg
     2821 ccggatcggc aggagtgaac tctacggctt gaagtacttc cgggccaccg tgcttgtgaa
     2881 attcaattcg tgttgccatg tgtcctccag aacgtatcgt cagggtctgc ttcatatggt
     2941 aaagtttcga cccattcttt atctcggtaa ctccattcac tatggcagga aataaaccct
     3001 tcaacaaaca gcaggctgaa ccccgcgaac gcgatccaca agttgccggg ctgaaagtgc
     3061 ctccgcactc gatcgaagcg gagcagtcgg tgttgggcgg tttaatgctg gataacgaac
     3121 gctgggacga tgtagccgag cgtgtggtgg cagacgattt ttacacccgc ccacaccgcc
     3181 atatctttac tgaaatggcg cgtttgcagg aaagcggtag tcctatcgat ctgattaccc
     3241 ttgcggaatc gctggaacgc caggggcaac tcgatagcgt cggtggtttt gcttatctgg
     3301 cagagctgtc aaaaaatacg ccaagtgcgg cgaacatcag tgcttatgct gacatcgtgc
     3361 gtgaacgtgc cgttgtccgt gagatgatct cggttgcgaa tgagattgct gaagccggtt
     3421 ttgatccgca ggggcgtacc agcgaagatc tgctggacct tgctgaatcc cgcgtcttta
     3481 aaattgccga aagtcgtgca aacaaagacg aagggccgaa gaacatcgcc gatgtgctcg
     3541 acgcaaccgt ggcgcgtatt gagcagttgt ttcagcagcc acacgatggc gttaccggag
     3601 taaacaccgg ttatgacgat ctcaacaaaa aaaccgctgg cttgcagccg tcggatttga
     3661 tcatcgtcgc cgcgcgtccg tcgatgggta aaacaacatt tgcgatgaac ctcgtcgaaa
     3721 acgcggcgat gttgcaggat aaaccagtac ttatcttctc gctggagatg ccttcagaac
     3781 agattatgat gcgttctctg gcgtcgctgt cgcgcgttga ccagactaaa atccgtaccg
     3841 ggcagctcga tgatgaagac tgggcgcgca tttccggcac catgggtatt ttgctcgaaa
     3901 aacgcaatat ctatatcgat gactcctccg gcctgacgcc aacggaagtg cgttcccgcg
     3961 cacgccgtat tgcccgtgaa cacggcggca tcgggcttat catgatcgac tacctgcaac
     4021 tgatgcgcgt accggcgctt tccgataacc gtacgctgga aattgcagaa atctctcgct
     4081 cgctgaaagc actggcgaaa gaactgaacg tgccggtggt ggcgctgtcc cagttgaacc
     4141 gttctctgga acaacgtgcc gacaaacgcc cggtcaactc cgacctgcgt gaatctggct
     4201 ctatcgagca ggatgcggac ttgattatgt ttatctatcg tgatgaggtg tatcacgaaa
     4261 acagtgattt aaaaggcatc gcggaaatta ttatcggtaa acaacgtaac ggcccaatcg
     4321 ggacggtacg cctgaccttt aacggtcaat ggtcgcgctt cgacaactat gcggggccgc
     4381 agtacgacga cgaataataa ttattttatg aattaggtaa ttaaagcaaa cacttatcaa
     4441 ggaacacaaa tgcaagcggc aactgttgtg attaaccgcc gcgctctgcg acacaacctg
     4501 caacgtcttc gtgaactggc ccctgccagt aaaatggttg cggtggtgaa agcgaacgct
     4561 tatggtcacg gtcttcttga gaccgcgcga acgctccccg atgctgacgc ctttggcgta
     4621 gcccgtctcg aagaagctct gcgactgcgt gcggggggaa tcaccaaacc tgtactgtta
     4681 ctcgaaggct tttttgatgc cagagatctg ccgacgattt ctgcgcaaca ttttcatacc
     4741 gccgtgcata acgaagaaca gctggctgcg ctggaagagg ctagcctgga cgagccggtt
     4801 accgtctgga tgaaactcga taccggtatg caccgtctgg gcgtaaggcc ggaacaggct
     4861 gaggcgtttt atcatcgcct gacacagtgc aaaaacgttc gtcagccggt gaatatcgtc
     4921 agccattttg cgcgcgcgga tgaaccaaaa tgtggcgcaa ccgagaaaca actcgctatc
     4981 tttaatacct tttgcgaagg caaacctggt caacgttcca ttgccgcgtc gggtggcatt
     5041 ctgctgtggc cacaatcgca ttttgactgg gtgcgcccgg gcatcattct ttatggcgtc
     5101 tcgccgctgg aagatcgctc catcggtgcc gattttggct gtcagccagt gatgtcacta
     5161 acctccagcc tgattgccgt gcgtgagcat aaagccggag agcctgttgg ttatggtgga
     5221 acctgggtaa gcgaacgtga tacccgtctt ggcgtagtcg cgatgggcta tggcgatggt
     5281 tatccgcgcg ccgctccatc cggtacgcca gtgctggtga acggtcgcga agtaccgatt
     5341 gtcggacgcg tggcgatgga tatgatctgc gtagacttag gtccacaggc gcaggacaaa
     5401 gccggggatc cggtcatttt atggggcgaa ggtttgcccg tagaacgtat cgctgaaatg
     5461 acaaaagtga gcgcttacga acttattacg cgcctgactt caagggtcgc gatgaaatac
     5521 gtggattaat cgttctgtaa tatttgattg tctgtgccgg atgcggcgtg aatgccttat
     5581 ccggccaata aaatcctaaa aattcaataa gttgatgttc tttcatgctc ttataaaggt
     5641 cgtgcctctg gcggatgttc gtttgtcatg agtctcactc tgttgctaat tgccgttcgc
     5701 tcctgaacat ccactcgatc ttcgccttct tccggtttat tgtgttttaa ccacctgccc
     5761 gtaaacctgg agaaccatcg cgtgtttcaa aaagttgacg cctacgctgg cgacccgatt
     5821 cttacgctta tggagcgttt taaagaagac cctcgcagcg acaaagtgaa tttaagtatc
     5881 ggtctgtact acaacgaaga cggaattatt ccacaactga aagccgtggc ggaggcggaa
     5941 gcgcgcctga atgcgcagcc ccatggcgct tcgctttatt taccgatgga agggcttaac
     6001 agctatcgcc atgccattgc gccgctgctg tttggtgcgg accatccggt actgcaacaa
     6061 cagcgcgtag caaccattca aacccttggc ggctcagggg cattgaaagt gggcgcggat
     6121 ttcctgaaac gctacttccc ggaatcaggc gtctgggtca gcgatcctac ctgggaaaac
     6181 cacgtagcaa tattcgccgg ggctggattc gaagtgagta cttacccctg gtatgacgaa
     6241 gcgactaaca gcgtgcgctt taatgacctg ttggcgacgc tgaaaacatt acctgcccgc
     6301 agtattgtgt tgctgcatcc atgttgccac aacccaacgg gtgccgatct cactaatgat
     6361 cagtgggatg cggtgattga aattctcaaa gcccgcgagc ttatcccatt cctcgatatt
     6421 gcctatcaag gatttagtgc cggtatggaa gaggatgcct acgctattcg cgccattgcc
     6481 agcgctggat tacccgctct ggtgagcaat tcgttctcga aaattttctc cctttacggc
     6541 gagcgcgtcg gcggactttc tgttctgtgt gaagatgccg aagccgctgg ccgcgtactg
     6601 gggcaattga aagcaacagt tcgccgcaac tactccagcc cgccgaattt tggtgcgcag
     6661 gtggtggctg cagtgctgaa tgacgaggca ttgaaagcca gctggctggc ggaagtagaa
     6721 gagatgcgta ctcgcattct ggcaatgcgt caggaattgg tgaaggtatt aagcacagag
     6781 atgccagaac gcaatttcga ttatctgctt aatcagcgcg gcatgttcag ttataccggt
     6841 ttaagtgccg ctcaggttga ccgactacgt gaagaatttg gtgtctatct catcgccagc
     6901 ggtcgcatgt gtgtcgccgg gttaaatacg gcaaatgtac aacgtgtggc aaaggcgttt
     6961 gctgcggtga tgtaatgcag gaaagcaggc tggagttacc cagcctgcag tgaaattaaa
     7021 ctgtcgtcgc tttcactatt tctttgtaga tgatttt
//
LOCUS       DAZOYL010000206         6957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZOYL010000206 DAZOYL010000000
VERSION     DAZOYL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.207"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            152..1333
                     /gene="tsgA"
                     /locus_tag="ACHUOY_003047"
     CDS             152..1333
                     /gene="tsgA"
                     /locus_tag="ACHUOY_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR0683767.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYSVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            1595..4138
                     /gene="nirB"
                     /locus_tag="ACHUOY_003048"
     CDS             1595..4138
                     /gene="nirB"
                     /locus_tag="ACHUOY_003048"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR0683768.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            4135..4461
                     /gene="nirD"
                     /locus_tag="ACHUOY_003049"
     CDS             4135..4461
                     /gene="nirD"
                     /locus_tag="ACHUOY_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR0683769.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            4587..5393
                     /gene="nirC"
                     /locus_tag="ACHUOY_003050"
     CDS             4587..5393
                     /gene="nirC"
                     /locus_tag="ACHUOY_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR0683770.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            5412..6785
                     /gene="cysG"
                     /locus_tag="ACHUOY_003051"
     CDS             5412..6785
                     /gene="cysG"
                     /locus_tag="ACHUOY_003051"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR0683771.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         1602 a         1783 c         1844 g         1728 t
ORIGIN      
        1 tcacttttta ttccgtgatc aaaatcacct cttaaaatgc aatttagcaa ccgattgcaa
       61 taaaacattt aaacagatca caaaatcacc taaaatcgcc cgctcgcggc atatcccgct
      121 atgccatttt atttttgaac gcaggccaat catgactaac agcaatcgca tcaagctcac
      181 atggattagc tttctctcct acgcactgac tggtgcgttg gttattgtca ccgggatggt
      241 gatgggaaat atcgccgatt atttcaatct gcctgtttcc agtatgagta acaccttcac
      301 cttcctcaac gccggcattt taatctctat cttcctcaac gcctggctga tggaaatcgt
      361 cccgttgaaa acgcagttac gttttggctt tctcctgatg gtgctggcgg ttgccggttt
      421 gatgttcagc cacagcctgg cgctgttctc gacggcgatg ttcattctcg gggtggtcag
      481 cggcattact atgtcgattg gtacattcct gataacacaa atgtatgaag ggcgtcagcg
      541 cggttcccgc ctgttattta ctgactcctt cttcagtatg gctgggatga ttttcccaat
      601 gatcgccgcg tttctactgg cgcgcagcat tgagtggtac tcggtttatg cctgcatcgg
      661 gctggtgtat gtcgctattt ttattctgac cttcggctgt gagttcccgg cactgggcaa
      721 acatgcacca aaaacggatg ctccggtaga gaaagaaaag tgggggatcg gcgtactgtt
      781 tctctctgtt gcggcgctgt gctacatcct cggtcagtta ggttttatct cctgggtgcc
      841 tgagtatgcc aaaggcctgg gcatgagcct gaacgacgcg ggcacgctgg tgagtaactt
      901 ctggatgtca tacatggtcg gcatgtgggc gttcagcttt attcttcgct tctttgattt
      961 gcaacgcatt ctgaccgtac tggctggtct ggctgcgatt ctgatgtacg tctttaacac
     1021 cggaacacca gcacatatgg cgtggtcaat tctcgctctg ggcttcttct ccagcgcgat
     1081 ctataccacc atcatcaccc tgggttcaca gcagaccaaa gtaccgtcgc caaaactggt
     1141 taactttgtc ctgacctgcg gaaccatcgg tactatgttg acctttgtgg ttaccggccc
     1201 gattgttgaa catagcggtc cgcaggcggc actgcttaca gcaaacggtc tgtacgctgt
     1261 cgtctttgtg atgtgcttcc tgttaggttt cgtcagccgt caccgtcagc ataacaccct
     1321 gacctctcat taattgctaa tgccggacgg cactatcgtc gtccggcctt ttcctctctt
     1381 cccccgctac gtgcatctat ttctataaac ccgttcattt tgtccatttt ttgcacaaac
     1441 atgaaatgtc agacaattcc gtgacttaag aaaatttata caaatcagca acataccact
     1501 taaggagtat ataaaggtga atttgattta catcaataag cggggttgct gaatcgttaa
     1561 ggtaggcggt aatagaaaag aaatcgaggc aaaaatgagc aaagtcagac tcgcaattat
     1621 cggtaacggt atggtcggcc atcgctttat cgaagatctt cttgataaat ctgatgcggc
     1681 caactttgat attaccgttt tctgtgaaga accgcgcatc gcttatgacc gcgtacacct
     1741 ctcgtcttac ttctctcacc acaccgccga agagctgtcg ctggtgcgcg aaggcttcta
     1801 cgagaaacac ggcatcaaag ttctggtcgg cgaacgcgct atcaccatca accgtcagga
     1861 gaaggtgatt cactccagcg ccggacgtac cgttttttat gacaagctga tcatggcaac
     1921 cggttcctac ccgtggatcc caccaatcaa aggttctgat actcaggact gctttgtcta
     1981 tcgcaccatt gaagacctca acgccattga atcctgcgcc cgtcgcagca aacgcggtgc
     2041 cgttgttggt ggcggcctgt taggtctgga agccgctggc gcgctgaaaa acttaggtat
     2101 tgaaacccac gttatcgaat ttgcccctat gctgatggcg gaacagcttg atcagatggg
     2161 tggcgagcag ctgcgtcgca aaatcgaaag tatgggcgtg cgcgttcaca ccagcaaaaa
     2221 cacccttgag attgtgcagg aaggtgtcga agcgcgtaaa accatgcgtt ttgccgacgg
     2281 cagcgaactg gaagtcgact ttatcgtctt ctctaccggt atccgtccgc gcgataagct
     2341 ggcaacccag tgtggtctgg acgttgctcc gcgtgggggt atcgtcatta atgattcctg
     2401 ccagacttcc gatccggata tctacgccat cggtgaatgc gcaagctgga acaaccgtgt
     2461 atttggtctg gtagcacctg gctacaaaat ggcgcaggtc gccgttgacc atattctcgg
     2521 tagcgaaaac gcctttgaag gtgctgacct tagcgccaag ctgaaactgc tgggcgtaga
     2581 cgtaggcggt attggtgatg cgcacggtcg cacgcctggc gcacgtagct acgtttacct
     2641 cgacgaaagt aaagagatct acaaacgcct gattgtcagc gaagacaaca aaaccctgct
     2701 cggtgcggta ctggtgggcg ataccagcga ctacggtaac ctgctgcaac tggtgctgaa
     2761 cgctatcgaa ctgccggaaa acccggattc cctgatcctg ccagcacact ctggtagcgg
     2821 caagccgtct atcggtgttg ataaactgcc ggacagcgcg caaatctgct cctgcttcga
     2881 cgtcactaaa ggtgatctga ttgctgccat caacaaaggc tgccacacag ttgcggcgct
     2941 gaaagctgaa accaaagcgg gtactggctg cggtggctgt atcccgctgg tcactcaggt
     3001 actgaacgcg gaactggcga aacagggcat cgaagttaac aacaacctgt gcgaacactt
     3061 tgcttattcg cgtcaggaac tgttccattt gatccgcgtt gaaggcatta aaaccttcga
     3121 agaactgctg gcgaaacacg gcaaaggcta cggttgtgaa gtttgtaaac caaccgtcgg
     3181 ttcgctgctg gcctcctgct ggaacgaata cattctgaag ccggaacata ctccgctgca
     3241 ggattctaac gacaacttcc tcgctaacat ccagaaagac ggcacctact cggtgatccc
     3301 gcgttctccg ggcggtgaaa tcaccccgga agggctgatg gcagtgggtc gtatcgcgcg
     3361 tgaatttaat ctctacacca aaatcaccgg ctcccagcgc ctggcgatgt ttggcgcaca
     3421 gaaagacgat ctgccggaaa tctggcgtca gctgattgaa gctggcttcg aaaccggtca
     3481 tgcctatgcg aaagcactgc gtatggcgaa aacctgcgtg ggtagcacct ggtgccgcta
     3541 cggtgttggc gatagcgtcg gcctcggcgt ggaactggaa aaccgctaca aaggcatccg
     3601 tacgccgcac aaaatgaagt tcggtgtctc cggctgtacc cgtgaatgtt cagaagctca
     3661 gggtaaagac gtgggtatta tcgccactga aaaaggctgg aacctgtatg tttgcggtaa
     3721 cggcggcatg aaaccgcgtc atgcggatct gctggcagcg gatatcgatc gcgaaacgct
     3781 gatcaaatat ctcgaccgct tcatgatgtt ctacatccgt accgccgaca aactgacgcg
     3841 taccgcgccg tggttagaaa acctcgaagg cggcatcgat tacctgaaag cggtgatcat
     3901 tgacgacaaa ctggggctga acgctcatct ggaagaagag atggcgcgcc tgcgtgaagc
     3961 ggtagtgtgt gagtggactg aaacggtcaa tacgccgtct gcgcagactc gcttcaaaca
     4021 cttcatcaac agcgacaagc gtgacccgaa cgtgcagatg gtgccagagc gcgaacagca
     4081 ccgtccggca acgccgtatg aacgtatccc ggtaactctg gtggaggaca acgcatgagc
     4141 cagtggaaag acatctgcaa aatcgatgac atcctgcctg aaaccggcgt ctgcgcgctg
     4201 ttaggtgacg agcaggtagc gattttccgc ccgtatcaca gcgaccaggt gtttgcgatc
     4261 agcaacattg atccgttctt cgagtccagc gtgctatcac gcgggctgat tgcggaacac
     4321 cagggcgagc tgtgggtcgc cagcccgctg aaaaaacagc gttttcgctt aagtgatggc
     4381 ttgtgcatgg aagacgaaca gttttccgtc aaacattacg aagcgcgagt gaaagacggc
     4441 gtggtgcagc tgcgcggtta atgttttaac gggaggcgca atgcctcccc tttttgcatg
     4501 gtcctgtaat gaccttcggt atattgcaga acatttttta aactttttgt tttatttttt
     4561 gtttttattt tttaaaggat aatcaaatgt ttacagacac tattaataag tgtgcggcta
     4621 atgctgcgcg cattgcacgc ctgtcggcaa ataacccgct cggcttttgg gtcagctccg
     4681 ccatggcggg cgcatatgtg ggtcttggga tcatcctgat tttcacgctc ggtaatttgc
     4741 tcgacccgtc cgtacgtccg ctggtgatgg gcgcgacctt tggtatcgcc ttaacgctgg
     4801 tgattatcgc cggttctgaa ctgttcaccg gacacgccat gttcctcacc tttggggtaa
     4861 aagcgggcag catcagccac gggcaaatgt gggcaatcct gccgcaaacc tggctgggta
     4921 acctggtcgg ttccgtcttc gttgccatgc tctatagctg gggcggcggt agcctgctgc
     4981 cggtagatac cagcatcgtt cactccgtcg cgctggctaa aaccactgca ccggcaatgg
     5041 tactgttctt caaaggtgca ttgtgtaact ggctggtttg cctggctatc tggatggcgc
     5101 tgcgcactga aggggcggcg aaatttatcg ctatctggtg gtgtctgctg gcatttatcg
     5161 cgtccggcta cgagcactcc atcgctaaca tgacgctgtt cgccctctcc tggttcggca
     5221 accacagcga agcctacacg ctggcgggta ttggtcataa cctgctgtgg gtgacgctgg
     5281 gtaatacttt atcgggtgcc gtattcatgg gattgggtta ttggtatgct acgccgaaag
     5341 cgaatcgtcc ggttgcggac aaatttaatc aaactgaaac ggctgccggt taattactaa
     5401 ggggttttta cgtggatcat ttgcctatat tttgccaatt acgcgatcgc gactgtctga
     5461 ttgtcggcgg tggtgatgtc gcggaacgca aagcaaggtt gctgttagac gcaggcgctc
     5521 gcttaacggt gaatgcatta gcgtttattc cacagttcac cgcatgggca gatgcaggca
     5581 tgttaaccct cgtcgaaggg ccatttgatg aaagccttct cgacacctgc tggctggcga
     5641 ttgcagcgac ggatgatgac acgcttaacc agcgcgtcag cgaagccgct gaagctcgtc
     5701 gcatcttctg taacgtagtc gatgcgccga aagccgccag ctttattatg ccgtcgatta
     5761 ttgaccgctc accgctaatg gtagcggtct cctctggcgg cacctctccg gttctggcgc
     5821 gcctgttgcg cgaaaaactc gaatcactgc tgccgttgca tctgggccag gtggcgaaat
     5881 atgccgggca attacgcggg cgagtgaaac aacagttcgc cacgatgagt gagcgtcgcc
     5941 gtttctggga gaaattgttc gttaacgacc gcctggcgca gtcgctggca aacaacgatc
     6001 agaaagccat tactgaaacg accgaacagt taatcaacga accgctcgac catcgcggtg
     6061 aagtggtgct ggttggtgca ggtccgggcg atgccgggct gctgacgctg aaaggactgc
     6121 aacaaattca gcaggcagat gtggtggtct acgaccgtct ggtttctgac gatattatga
     6181 atctggtacg ccgcgatgct gatcgcgttt tcgtcggcaa acgcgcggga taccactgtg
     6241 taccgcagga agagattaac cagatcctgc tgcgggaagc gcaaaaaggc aaacgcgtgg
     6301 tgcggctgaa aggtggcgat ccgtttattt ttggccgtgg tggcgaagag ctggaaacac
     6361 tgtgcaacgc gggtattccg ttctcggtgg ttccgggtat taccgcagct tctggttgct
     6421 ctgcctattc gggtattcca ctcacgcatc gcgattatgc ccagagcgta cgcttaatta
     6481 ccggacactt aaaaaccggt ggcgagctgg actgggaaaa cctggcggca gaaaaacaga
     6541 cgctggtgtt ctatatggga ttgaatcagg ccgcgactat tcagcaaaag ctgattgaac
     6601 acggtatgcc tggcgaaatg ccggtggcaa ttgtcgaaaa cggaacggca gtcacgcagc
     6661 gcgtgattga cggtacgctc acgcaactcg gtgaacttgc tcagcaaatg aacagtccat
     6721 cgctaattat tattggtcgg gttgttggcc tgcgcgataa actgaactgg ttctccaacc
     6781 attaatttaa cccgggccag agaattctgg ccttcttaat gactcttttt atatattcag
     6841 caaataaaac ataacccctt aataaatatt atctgctaat gggttatatt caccgttaca
     6901 gcgacttaca aaattaagtc attcgctcac tgacgccaat taaatattcc tgtatcg
//
LOCUS       DAZOYL010000207         6912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZOYL010000207 DAZOYL010000000
VERSION     DAZOYL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.208"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            121..1899
                     /locus_tag="ACHUOY_003052"
     CDS             121..1899
                     /locus_tag="ACHUOY_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR0683772.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1939..2418)
                     /gene="xdhC"
                     /locus_tag="ACHUOY_003053"
     CDS             complement(1939..2418)
                     /gene="xdhC"
                     /locus_tag="ACHUOY_003053"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR0683773.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2415..3293)
                     /gene="xdhB"
                     /locus_tag="ACHUOY_003054"
     CDS             complement(2415..3293)
                     /gene="xdhB"
                     /locus_tag="ACHUOY_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR0683774.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3304..5601)
                     /gene="xdhA"
                     /locus_tag="ACHUOY_003055"
     CDS             complement(3304..5601)
                     /gene="xdhA"
                     /locus_tag="ACHUOY_003055"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR0683775.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKRDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6016..6771
                     /gene="actS"
                     /locus_tag="ACHUOY_003056"
     CDS             6016..6771
                     /gene="actS"
                     /locus_tag="ACHUOY_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR0683776.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
BASE COUNT         1732 a         1655 c         1785 g         1740 t
ORIGIN      
        1 aaatgataaa aatcttatgt ttttatcaaa gccaatttcg cctgagttaa tatcatcatg
       61 atctgtgaac catcaacgtc ttcaatgatc gacttttaag taagtctgga aggtaaagct
      121 atggagcttg ctactacgca gtcagtattg atgcaaattc aaccgacaat tcagcgtttt
      181 gccagaatgc ttgccagcgt tttgcagttt gaggttgaga tcgttgatga aaacttgtgt
      241 cgcgtcgccg gaacgggcgc gtatgggaag tttcttggtc gccagttgag cggcaactca
      301 cgcctgctcc gccacgtcct ggaaacgaaa actgaaaaag ttgtgacaca gtctcgcttc
      361 gatccccttt gcgaaggttg cgatagtaaa gaaaattgcc gcgaaaaagc atttctgggt
      421 acgcctgtca ttttacagga tcgttgtgtt ggggtgataa gtttgattgc cgttacccac
      481 gagcaacaag agcatatcag tgataattta cgcgaatttt ctgattatgt tcgccatata
      541 tccaccattt ttgtttcgaa acttctggag gatcaggggc caggagataa catcagtaaa
      601 atattcgcga ccatgatcga taatatggat cagggcgtat tagttgttga tgatgaaagt
      661 cgggttcagt ttgttaatca gactgcctta aaaacacttg gtgttgtaca aaataatatt
      721 attgggaaac ctatccgttt cagaccatta acatttgaga gtaattttac tcatggacat
      781 atgcagcata ttgtttcgtg ggacgataaa agtgaattaa tcattggtca attgcataac
      841 attcagggcc gacaattatt tttaatggca tttcaccaat cgcataccag tttttctgta
      901 gcaaatgcac ctgatgaacc acatattgaa caattggttg gcgagtgccg tgttatgcgg
      961 caattaaaac gactcattag ccgtattgca cccagcccat ccagcgttat ggtggttggt
     1021 gaaagcggca cgggtaaaga agtcgtcgcc cgagcaatcc ataagttgag cggaagacgg
     1081 aataaaccct ttattgctat caactgtgcc gcgattccgg agcagcttct ggaaagcgaa
     1141 ctgttcggtt atgttaaagg cgcatttact ggtgcttctg ccaacggtaa aacagggttg
     1201 attcaggcgg cgaatacggg cacgctgttt ctcgatgaaa taggtgatat gccattaatg
     1261 ttgcaggcta aattactgcg cgctattgag gcgcgtgaaa ttctgccgat tggtgccagt
     1321 agcccaatac aagtcgacat tcgcatcatt tctgcaacta atcagaattt ggcccagttc
     1381 attgccgaag gtaaattccg cgaagatctc ttctaccgac ttaatgttat cccgataact
     1441 ctgccaccgc tgcgtgaacg tcaggaagat attgaactat tggtgcatta ctttttacat
     1501 ctgcataccc gtcgtctggg atcggtttat cctggcattg ctcccgatgt cgtcgaaata
     1561 ttgcgtaagc atcgttggcc cggaaacctg cgcgagttaa gcaatttgat ggaatatctg
     1621 gttaacgtgg ttccttcagg tgaagttatc gacagcacgc tattgccgcc aaatctgctg
     1681 aataatggca caacggagca aagtgatgta acagaggtca gtgaggcgca cctgtcactc
     1741 gatgatgcgg gcggcacggc gctggaggag atggaaaagc aaatgatccg cgaggcgctt
     1801 tcacgtcata acagcaagaa gcaagttgct gatgaactgg gcatcggcat tgctacgctc
     1861 tatcgcaaga ttaagaaata tgagttgtta aacacataac ctcagtgaat ccggcctgaa
     1921 ttcaggccgg atatcctttt acttcgtttt ctcgcaatcc agaactgtat ttacaatcat
     1981 ctgatacccc gtgcagcgac aaagatttcc cgccagtccg cgacgaattt ccgtaatggt
     2041 taatggcttc tcgcgtggtt tcgccagcat tgccgtggta gccataatca ggccaggcgt
     2101 acaaaacccg cactgcactg cgccggattt cgcataagcc tgctgaacat gagaaagttt
     2161 tccgcctttc gcttcacctt ccagcgtgcg gatctccttt ccttcagccc aggcggcaag
     2221 gtataagcaa ctgtctattg ctgtgccgtc gaccaacacc gtacaggcac cacattcacc
     2281 cacgcagcac ccttgtttga cacttagcag tccttgttcg cggagtaatt ccgagagcgg
     2341 cgtgcctggt gcggcgtgaa gctgaaaagg catcccgtta atggtgcatt cgatggtaat
     2401 tgtttcgctg tgattcattg caattttccc cccgccgcgg cgacggcttc gctaatcact
     2461 tttttggtca tcgtctggat gagatgcaga cgaaactctt tactggcccg ccatgaagaa
     2521 cgcggggcga catcttgcag gacagattcg ctgatagctt ccagcgtttg caggtttaat
     2581 ggcgcatttt gtgcagtctg ttcggcatgt tggcagcgaa tcggcgttgg cgcggcaaca
     2641 ccaaatgcca ggcgtaattc gctgaaattg ccgttatcca gtcggcaatg tgcggcgcag
     2701 ccaatcgttg aaatatccat tgcgtcgcgc atggcatatt taaaatgcgc gctgcccgcg
     2761 tgttctttcg gctgtggcgg aaaatgaaag gcgacgagga tttcgtcatg ctcaagagac
     2821 actttgcccg gcccggtgtg aaagccatta atcgggacga aacgaacacc gcgcggggag
     2881 tggatctcca gtttcgcgtc ataaattagc gttggcgtgg cagaatctgc gctggtggca
     2941 ccgttgcaaa tatttccacc gtaggtagcg acgttacgga tctgcggtcc agcaatggac
     3001 gtggccgcag cacataacgc cgggagatga cgttgagtta taggagcttc tattagctgg
     3061 gtaaatgtcg ttgcagagcc gatacgtagc gagccatctt ccgccagcgt aattccccgc
     3121 agctccgcca gattatgaat atcaacaata tggcgataac ggtcattgtg atggtggagc
     3181 tgaatcagta cgtcagtgcc atcggcgagc agtttggcct gcgggttgtc agccagcagg
     3241 ttgatggcat cggcaagggt tgctgcgcga tggtaagaag caaaatcaaa catgatgtta
     3301 tcctcaaatc aatcctgcca gatggaactc ttcatataac cgttttggcg tcagcggcag
     3361 tgtattgatt gcaacaccgg tagccatctt caccgcgtta cgaatagcag cggcaacagg
     3421 aattattggt ggctcaccca gtgacttatg tccgtatgcg gattgcggct cattgatttc
     3481 gacgaacgcg ctttccagtt gtggcagatc cggcatggtc ggcattttgt aatccagcag
     3541 attggggtta cggaccacgc cgcttttagc atcgatgatc atctcttcaa atagcgccca
     3601 gccaatgccc attcccattc cgccgtgtac ctgaccttct gccagcagtg gattaagaat
     3661 atgccctgaa tcatgaacgt tgaggatgcg gttgatggtg actttgcaca gcgcaatatc
     3721 gaccgtcaga tcaacaaagg tacagccaaa cgccgatggg ttagtggtgg ttttgatgga
     3781 gctttcagca gagagctgcc cgccgcgttc agggtggtag aaagcgtcca tcgccaaatc
     3841 ttttaacgac attaacggct cttccggtcg ttcaaccagc acgatatggc cttttatcag
     3901 ggtcagattc atcgctgact gatgtagcat gactgcggcg tgagcgatga ttttctcttt
     3961 taataatagt gccgcactgc gcagcgcagg cgcggcaaca tagctctggc gtgaggcaaa
     4021 tgcgccggga gcgaacggcg taacgtcggt atcttgagtt gaaataacgc gaacgtcgct
     4081 gaccggaacc cccacggttt ctgccaccat ttgcgagaag acggtgtcgg caccctgacc
     4141 gatttccgtc gcgccgcttt gcacgttgat ggttccatcc tgattcatca gaaggcgcgc
     4201 gcctgctatt tctacgccga caggccaggt gttagaggta tagctaaaac aggcgacgcc
     4261 aacgccgcgg cgcaaattgc cttgctggtt ctggcattct gcacggcgtt tttcccattc
     4321 aaagattttc cggccttttt caagacactc cggcaaccct gcgctgtaaa tacgtttgcc
     4381 cgtgagcgga ttagcatctc cttcgcgggc ggcgttgcgt aaacgaattt caacaggatc
     4441 aatacctaac gctgtcgcgg cgtcatcaag catagactca acggcaaata cgacttgtgg
     4501 cgcgccataa ccacgcatcg caccagccga ggggaggttg gtatagcagg tctttgaact
     4561 gtaagcgtag gcacaacgag gataaaggta agcgacttta ttcctccccg cagaagcgat
     4621 ggagtgcccg tgagatgcat aagcgccggt gttagacaga acatccagac tataaccttt
     4681 caatgttccg tcgcggttca cgcccatttg cccgtcaatg gtaaaagcgt ggcgggtacg
     4741 ggttgcgagg aaacactctt cacggctaag ggaaactttc accggaatgc cgccaagctt
     4801 gctggtcagg aatgctgcca ttggctcttc cagtacatcc cgtttattac caaaaccgcc
     4861 accgacaaac ggtttgatga ctcgtacgca tgaccaggga atatccagcg cctgaccaac
     4921 cacgcggcga acaatgtgcg ggatctgggt gctggaaacg atggtaattc gcgagtcatc
     4981 ctccatccac gccagcgatg ttacgctttc catgtgacaa tgttgaataa cgggggtctg
     5041 atagtgcccc tgtacctggt agtcggcggc atcgattgtt tgttggacat tacccgtcga
     5101 catcgtgctt tgtttcagta aattgccacc gttatggatt ggtgcagcgt cttctgccag
     5161 cgccgcttct ggcgtggtga taacgggtaa ttcttgccac tcaatgctga ccaattgcgc
     5221 cgctttttct gccgtgagtt catcgcgggc cacgacgatg gcaacggcgt caccatgatg
     5281 acgaacatgg cgagttagca gtgcgcgatc ggcggtatcg cgcttgtttt cgtcaagtgt
     5341 ccaggcatgc ccagctgtag cgaatggaat atcaggcaca tcttcccagg taaaaatcgc
     5401 cagtacgccc ggcaaacttc tggcttgttc atcattaata cttacggcat aaccatgtgc
     5461 gatagggcta cgtacatatt tcgcataaca catgcccgcc ataacataat cgtcagtata
     5521 tcgtgcccgc ccggtgacct tagcaatggc atcgacgcgc atgcatgatt cacccgtagc
     5581 ggttgcttcc cgcgcttcca tagaaatccc ctcgatttga acatttggtt cttatggcaa
     5641 agatttagtc taagcaggca agatttacgc cagaaacgac gataagcgtt aggggaggta
     5701 ggaagtgtga tgacgttaaa aaaacacaga tatgatacga aacaggactc tgaatagggg
     5761 cagtgatacg tatcaatatg agtggcgata aaatcaatgt gataaattgg gattattacg
     5821 catcaggatg ggaaataggg taaaaagtca ggaagttcga tacctctaca gcgaatattt
     5881 tgctgatgat acttcccttt gccaaatgaa attaacctct ttctctatta ccacgttttt
     5941 ccagaagcaa gagattgggt atcaagaggc tggctgctat gataaggcgt cttgtttttt
     6001 aagcgaggaa agattttgag tgcgggacgc ctgaataaaa aatctctggg tatcgtgatg
     6061 ttgttatcgg ttggactgct tttggcgggc tgttcgggta gcaaatcatc cgatacagga
     6121 acgtattccg gctccgttta caccgtgaaa cggggggata cgctatatcg tatttcgcgc
     6181 accacgggaa ccagcgtaaa agaactggcg cgactgaacg gcatttcccc cccttacacc
     6241 attgaagttg gtcagaaact aaaactgggt ggggcgaaaa gtagcagtag tacacgtaaa
     6301 tcaaccgcca aatcaacgac caaaaccgca tcggttacac cgtcatcagc ggtaccgaaa
     6361 tcatcctggc cgccagtagg gcaacgttgt tggttatggc caacgacagg gaaagttatc
     6421 atgccgtatt cgacagcaga tggcggcaat aaagggattg atatctcagc tccacggggt
     6481 acacctattt acgccgcggg tgcaggaaag gtggtgtatg tgggcaacca gctgcgtggc
     6541 tacggtaatc tcatcatgat taaacacagt gaagattaca ttacggctta cgcccataat
     6601 gacacgatgc tggtaaataa tgggcaaagc gtgaaggctg ggcaaaaaat cgccactatg
     6661 gggagcacgg atgcggcatc tgttcgcctg catttccaga ttcgttaccg tgcaacggca
     6721 attgatccgc tacgttactt gccgccgcag ggcagcaagc caaaatgctg atggcgaatt
     6781 aatcagcagt cagcagcgcg gcacttgcta aggagagcgt aaggtttata atgccttacg
     6841 catctcgaag cgggcgtagt tcaatggtag aacgagagct tcccaagctc tatacgaggg
     6901 ttcgattccc tt
//
LOCUS       DAZOYL010000208         6900 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZOYL010000208 DAZOYL010000000
VERSION     DAZOYL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.209"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            110..382
                     /gene="asr"
                     /locus_tag="ACHUOY_003057"
     CDS             110..382
                     /gene="asr"
                     /locus_tag="ACHUOY_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR0683777.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            475..558
                     /locus_tag="ACHUOY_003058"
     CDS             475..558
                     /locus_tag="ACHUOY_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683778.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            658..1479
                     /locus_tag="ACHUOY_003059"
     CDS             658..1479
                     /locus_tag="ACHUOY_003059"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR0683779.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="ACHUOY_003060"
     CDS             complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="ACHUOY_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR0683780.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="ACHUOY_003061"
     CDS             complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="ACHUOY_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR0683781.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2611..3645
                     /gene="tqsA"
                     /locus_tag="ACHUOY_003062"
     CDS             2611..3645
                     /gene="tqsA"
                     /locus_tag="ACHUOY_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR0683782.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="ACHUOY_003063"
     CDS             complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="ACHUOY_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR0683783.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="ACHUOY_003064"
     CDS             complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="ACHUOY_003064"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR0683784.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
BASE COUNT         1765 a         1824 c         1641 g         1670 t
ORIGIN      
        1 ccattcccag ggatatagtt atttcaacgg ccccgcggtg gggttaaatg aaaaaacaaa
       61 ttgagggtat gacaatgaaa aagtattagc tctggttgtt gccgctgcta tgggtctgtc
      121 ttctgccgcc tttgctgcag agactgcgac cacacctgct ccgactgcga cgaccaccaa
      181 agcagcgccg gcgaaaacga cacatcataa aaaacagcat aaagcagcac ctgcccagaa
      241 agcgcaggcg gctaaaaagc atcataaaaa tacgaaagct gaacagaaag cccctgaaca
      301 aaaagcgcag gcagcgaaga aacacgccgg gaaacacagc catcagcaac cggcaaaacc
      361 tgctgcacaa cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg
      421 aaaattacgg tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg tagtatggtg
      481 ggccgttatc gctttgagtt cattcttatc atccttattt tatgcgcact gattgccgcc
      541 cgtttttatc tttcctgact gtagttatct gattttactc ccactttcat cccgtcccgt
      601 ctatagtatt tatgagggtt tgcttttaat aatcataatt acccaccaga gtgtgatatg
      661 cgtacaacca ttgctgtagt gttgggtgca attagtttga cgtctgcttt tgtgtttgca
      721 gataaaccag acgttgccaa atcggcaaac gatgaggtca gcaccctgtt ttttggtcat
      781 gatgatcgtg tgccagtgaa tgacacgacc caatcaccgt gggatgcggt tgggcaactg
      841 gaaacggcca gcggcaattt atgtacggca acgctgattg cacccaatct ggcattaacg
      901 gcaggacact gtttattgac acctccaaag ggtaaagccg ataaagcagt ggcgctgcgt
      961 tttgtgtcaa ataaaggtct ttggcgctat gagatccacg acatagaagg ccgcgttgat
     1021 ccgacactgg gaaagcggtt aaaagcagat ggggatggtt ggattgtacc tcccgcagcc
     1081 gcgccgtggg acttcggatt gattgtgcta cgtaatcccc cttctggcat tacaccgttg
     1141 ccgttatttg agggagataa agccgcgctt actgccgcat taaaatcggc tggccgtaaa
     1201 gtaactcagg cgggataccc tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt
     1261 gaagtgactg gctgggcgca aacgtcggtg atgtcacatc agtgcgatac cttgccgggt
     1321 gacagcggtt cgcctctgat gttgcatacc aatgacggct ggcaattaat tggggtgcaa
     1381 agttcggctc ctgccgcgaa agatcgctgg cgcgccgata accgggccat ttctgttacc
     1441 ggttttcgcg acaagctgga tcaactgtcg caaaaataat gttcaaacgc tgcccgactg
     1501 tgcgggcagc gtgttcatca ggcaagtttc accatgatca ttccagccaa cagcaagacc
     1561 aggccaatcc agcctttacg atttaaccgt tgaccaaaca agatccaacc tgcggctaac
     1621 gtggcggcaa taccaaaccc gccccacaat gcataagcga cagacaagtc gatcccttta
     1681 acggcctgag aaagcgcact aaaggcagcc agcaccgccg ccagggagag caagccaaat
     1741 attttgcgac gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat ttccagcacg
     1801 attgccaatg ccagccaggc ggcgtgaacc cattcaaact gcgccatggt tcacctccag
     1861 ttcaggttta cgcgctttac gggtacctga ttttatcaac acaatcccgg cgaccagggt
     1921 ggttaacccg gcaattttca tcagcgataa actttcgtcg aataacaaaa cgctaaacaa
     1981 ggtaataaat aaaataccga taccttccca cagcgcataa gctacgccta aggcgatctt
     2041 tttaacggcg aaagagagaa atatatacga cagagaaatc atcaccagca ttaaaataaa
     2101 gccgccattt ccctcactga cgctcgccca tttcattgac agcgtaccgg taatttctgt
     2161 agcaatagcc aggcctaata aaatccaata aatatacatt gtccttctcc tgcaagagaa
     2221 ttattttaat tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgcata
     2281 gggcaagctt aggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga
     2341 gcaagatagt ggatgaggta cgaagatggg taaatgttga aaataacgtc ctgaacaaat
     2401 tgtccataat attacaatta tccgcagtgt tgcttctcgt catcgcggat gataattgtc
     2461 ctcggtagtt gaacacgcct gatttgtatc atagcttaag aattaactca aaatattttc
     2521 acttctttac ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa gacattatta
     2581 ttagcaaagg aagaaaaaac ggggacaagc atggcaaagc cgatcatcac gctcaatggc
     2641 ctaaaaatcg tcattatgtt gggaatgctg gtcattattc tctgcggtat ccgttttgcc
     2701 gccgagatca tcgtgccgtt tattctcgca ttatttattg ctgttattct taacccgctg
     2761 gtgcaacaca tggtccgctg gcgagtgccg cgtgtactgg cggtgtcgat tttgatgacc
     2821 atcatcgtga tggcgatggt gttgctatta gcttatctgg gttccgcgct caacgagttg
     2881 acgcggacgt taccgcaata tcgcaactct attatgacgc cgctgcaagc tcttgagccg
     2941 ttgttgcaac gcgtagggat tgacgtctca gttgaccagc tggcgcatta cattgatccg
     3001 aacgcggcga tgacgttgct caccaactta ttgacgcagt tatctaatgc catgtcatca
     3061 atatttttat tgctgctgac ggtgctgttt atgctgctcg aagtgccaca attgcccgga
     3121 aaatttcagc aaatgatggc gcgtccggtt gaagggatgg cggcgattca acgtgcaatt
     3181 gacagtgttt ctcattatct ggtgctgaaa acagccatca gcatcatcac cggcctggtc
     3241 gcctgggcga tgctcgccgc actcgatgtt cgcttcgctt ttgtctgggg attgctggcc
     3301 tttgcgctta attacatccc taatattggt tcagttctcg cggcaatccc ccctatcgct
     3361 caggtactgg tgtttaatgg cttctacgaa gcgttgctgg tgctggcggg atatctgctg
     3421 attaatctgg tcttcggcaa tattctggag ccgcgcatca tgggacgtgg gctggggctt
     3481 tccacattgg tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt
     3541 atgctgattt ccgtgccgtt aacaattatt gtcaaaattg cgcttgaaca aacagcggga
     3601 ggtcaaagca tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg
     3661 ccgtcagggt tacagagctt tcaggattgc atccacgcta gctttggcgt caccaaacag
     3721 catgtgggtg ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat
     3781 cgaacgttta aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc
     3841 aataggactc ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac
     3901 cagtacggta tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc
     3961 atacggtact tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac
     4021 cgggtggata ccgaaacgca cgttgatacc acgagcgcgc agtttctcgg taatttcagc
     4081 gacaggatat tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg
     4141 ggagtttttc agcagttccg ctgtctcttc tgcggtgatt tcgcggtgct cgcccacttc
     4201 ctgatcatcg ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa
     4261 ggaacggttc atcgccttac acataatgta agaaaggata gcccccgaag aaccgaccag
     4321 cgcaccggtc acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca
     4381 gccggagtac gagttcagca tcgacaccac cactggcata tctgcaccac cgatggaggc
     4441 gactaaatgc cagccgaata ccagcgcaat ggcggtcatt attagcaatg ccagcacttg
     4501 caggccgacg ctgtccgtgc gaacaaatac aatcagcagc aggaaggaaa cgaccagagc
     4561 cgccaggttc attttgtgac ggtttggcag catcaacggt ttagacgaaa tcttgccaca
     4621 cagtttgccg aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag
     4681 gaacacttcc gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag
     4741 atagctgtta aagccaacca gcactgccgc cagacccacg aagctgtgca ggatcgccac
     4801 cagttctggc atttcggtca tttcaacttt cttcgccaga cggataccaa ttgccccacc
     4861 aatgaccatc gccagcaaga tccagccaac attacccgta tccggtccaa aaatggttgc
     4921 gattaacgca atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg
     4981 tttcgaaaga ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt
     5041 aactaatcct ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct
     5101 gagtcacggt gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa
     5161 ggaagctaac ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg
     5221 aaatcgcgtt ggtgaccgac atcaacggtg tatgcagcgc gtgcgataca ttccacacca
     5281 cgtaataacc gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg
     5341 gcgcaacgct tgccagccaa ccgaaaagga tgattgccag cgccatcaac gcgtatttac
     5401 gccacggtga gcaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgccg
     5461 cctgcggctg agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga
     5521 tcacggtcac gccgcgaatc accacatcat caaaatcaac agtgatattg ccgtctttct
     5581 ctttgcacaa cagtttcagc agattaacga ggttagtacc gtaaagctgc gaggattgcg
     5641 tcggcagacg gcccggaaga tcggtataac caatcacttt gacaccattt tccgtagtga
     5701 agatttcacc cggcacggtg tattcacagt tgccaccgtt ttgggctgcc aggtcgacaa
     5761 tcacactgcc cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt
     5821 tgcctggaat aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga
     5881 gttccatttc cgctttaatg aacgcgtccg acatcacttt ggcatagcca tcgccgctgc
     5941 ccgcttcctc tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt
     6001 tcacttccgg acgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa
     6061 tggcggccag acctgcaaca cccgcaccaa tcaccatcac ttttgccggt ggcactttcc
     6121 cggccgcagt aatttgcccg gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg
     6181 cgcgataacc ggcgatgttc gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga
     6241 tacgcggcac agaatccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta
     6301 attccggatt ctgcgcaggc cagataaaac tcaccagcgt tgtcccagga ttcagtaacg
     6361 caatttcatc atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat
     6421 tcccttctac aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt
     6481 gacccgcgcc gctctctacc gcgacggtaa aacccagttt cagcagctgt tccactgttt
     6541 ttggcgttgc tgcaacacgg gtttcattgg ttaaccgttc tcttggtatg ccaattcgca
     6601 tgatattccc ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag
     6661 ccgctcatgt acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata
     6721 acgtactgaa aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg
     6781 caaaatatta acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga
     6841 ctgataattg tcttcgacgg ccgggtaaaa cgagacatcg ccccggcacg attcactact
//
LOCUS       DAZOYL010000209         6810 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZOYL010000209 DAZOYL010000000
VERSION     DAZOYL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.210"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..2657
                     /gene="cas3"
                     /locus_tag="ACHUOY_003065"
     CDS             <1..2657
                     /gene="cas3"
                     /locus_tag="ACHUOY_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR0683785.1"
                     /translation="TFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFG
                     LADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEKI
                     SHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGWPPDRMDELDN
                     FLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSS
                     TRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQ
                     KVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANAM
                     YDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCA
                     AWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMS
                     CILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQEAPFLEKDDYPWLT
                     HVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDAI
                     KVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDSSQRAGKVLICTQV
                     LEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAP
                     VWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGED
                     VVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVS
                     LWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQD
                     PEMANVILVNDDESCGYSATEGLIGKVG"
     gene            2755..4316
                     /gene="casA"
                     /locus_tag="ACHUOY_003066"
                     /gene_synonym="cse1"
                     /pseudo
     CDS             2755..4316
                     /gene="casA"
                     /locus_tag="ACHUOY_003066"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            4313..4849
                     /gene="casB"
                     /locus_tag="ACHUOY_003067"
                     /gene_synonym="cse2"
     CDS             4313..4849
                     /gene="casB"
                     /locus_tag="ACHUOY_003067"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR0683786.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            4861..5916
                     /gene="cas7e"
                     /locus_tag="ACHUOY_003068"
     CDS             4861..5916
                     /gene="cas7e"
                     /locus_tag="ACHUOY_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR0683787.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5927..6673
                     /gene="cas5e"
                     /locus_tag="ACHUOY_003069"
     CDS             5927..6673
                     /gene="cas5e"
                     /locus_tag="ACHUOY_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR0683788.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6598..>6810
                     /gene="cas6e"
                     /locus_tag="ACHUOY_003070"
     CDS             6598..>6810
                     /gene="cas6e"
                     /locus_tag="ACHUOY_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0683789.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
BASE COUNT         1690 a         1617 c         1856 g         1647 t
ORIGIN      
        1 tgactttttt tgatttctgg ggaaaaaccc gacgcggcga gaaagacggg ggcgacgact
       61 atcaccttct ttgctggcat tcgctggatg tagccgcgat gggctattta atggttaaaa
      121 gaaattgctt cgggctggct gattattttc gtcaattagg gatttctgac aaggaacagg
      181 cggctcaatt tttcgcctgg ttgctgtgct ggcacgatat tggaaaattt gcccgctctt
      241 ttcaacaact ttacctgccc cctgaactca agattcagga aggtgccaga aaaaattacg
      301 aaaaaatctc tcattcaacg ctgggctact ggttgtggaa tcattattta agtgaatgtc
      361 aggagttact tccttcatct tcgctctctc cgcgcaaact taggcgagtg atagaaatgt
      421 ggatgcccgt gacgacaggg catcatggtt ggccccctga tcgtatggat gaattggata
      481 attttctgcc tgaagacaaa gctgcagcac gagattttct ccttgaaatc aaaccactgt
      541 ttccgctcat agagatcccc gcattctggg atgatgatga gggcattgaa cttataaaac
      601 acctttcctg gtatatctct gcaaccgtcg tgctcgcaga ctggacgggt tcgtcaacgc
      661 gattttttcc acgcgtcgca cacccaatgg atattaaagg ttactggcag aaaactttag
      721 ttcaggctca aaacgcctta accgtctttc ctctaaaagc aaaagtcgcg cctttcaacg
      781 gaattaatac gctgtttcct tttattgaga atccgacacc gttacagcaa aaggtgctgg
      841 atctggatat cagccagcaa ggaccgcagt tatttattct ggaagatgtg actggcgcag
      901 gtaaaacaga agcggcgctt atcctggcgc acaggttgat agctgcgggg aaggctcagg
      961 ggctgttttt tggtctgcca acaatggcaa cggccaatgc catgtacgat cggctggtca
     1021 aaacctggct tgctttctat tcgccagaat cccgccccag cctggtgctg gcacatagtg
     1081 cccgcacatt aatggaccgc tttaatgaat cactctggtc tggtgattta gtcgggtcag
     1141 aagaaccgga tgaacaaacg ttcagtcagg gatgtgcggc ctggtttgcc gacagtaaca
     1201 agaaggcgtt gctggctgaa attggcgtcg gcacgctgga tcaggcgatg atggcggtga
     1261 tgccgttcaa acataataat ctgcggcttc tggggctgag taacaaaatc ctgctggctg
     1321 atgagatcca cgcctgtgac gcttacatgt cgtgcattct tgaagggttg atcgagcggc
     1381 aggcgcgtgg cggaaacagc gttattttgc tttctgccac gttatctcaa cagcagcgcg
     1441 acaaactcgt cgctgccttt gcgcggggta cagagggcct gcaagaagct ccgttcctgg
     1501 aaaaggatga ttacccctgg ctgacgcatg tcacgaaatc cgatgtgaac tcacaccggg
     1561 tagcgacgcg caaagacgtt gagcgtagcg tcagcgtggg ttggcttcat agtgaacaag
     1621 agtgtattgc gcgtatcgaa tcggcggtaa gtcaggggaa atgcatcgcc tggatccgga
     1681 attctgtcga tgacgctatt aaggttcatc gtcagctgct tgcccgcggc gtcattcctg
     1741 cttccagcct ttcacttttt catagccgct ttgcttttag cgatcgccag cgaattgaaa
     1801 cggagacact ggcacgcttt ggtaaagaag atagctcaca gcgtgccgga aaagtcctta
     1861 tttgtactca ggtattagag cagagtgtcg attgtgacct ggacgaaatg atctccgacc
     1921 tggcccctgt tgatttgctg attcagcgag caggacgatt acagcggcat atccgcgata
     1981 ttaatggtca gttaaagcgt gacggaaagg acgagcgttc cccccctgaa ttgctgattc
     2041 tggctcccgt ctgggacgac tctcctggcg acgaatggtt tggcagtgcc atgcgtaaca
     2101 gtgcatttgt ctatcccgat catgggcgaa tctggctgac gcagcgtgtg ctgcgtgagc
     2161 aaggtgctat tcagatgcca cacgcagccc ggcttcttat tgaatccgtc tacggtgagg
     2221 acgtggtaat gccggaagga tttgcccgca gcgagcagga gcaagtgggc aaatattact
     2281 gtgatcgcgc aatggctaaa aagtttgtgc tgaacttcag gcccggctat gccgccaata
     2341 tcaacgatta cctcccagaa aaactgtcga cacgtctggc tgaggagtct gtttccctgt
     2401 ggctggctac ctgtattgac ggtgtggtga agccttatgc caccggtgct cacgcatggg
     2461 aaatgagcgt tgtcagagtg cgccgaagct ggtggaaaaa acatcgggat gagttttctt
     2521 tactggaggg agaggcgttc aggctgtggt gcattgaaca gcgacaagat ccggagatgg
     2581 caaacgttat tttagtcaac gatgacgaaa gttgtgggta ttcggctacg gaaggattga
     2641 ttggcaaggt tggttgagat gtctgcgaca ggactctctg aatagttgat acaccgaaaa
     2701 ggatggaaag atggacaacc gacctgggat ggtctttttt acgagaggat gcgtatgaac
     2761 tcgttttcac ttctgacaac cccgtggttg cccgttcgtt ttaaagacgg aacgacaggc
     2821 aaactggcgc cagtcgatct ggcggatgaa aatgttgtcg atatttctgc gccgcgggca
     2881 gatctccagg gtgcggtatg gcagtttttg ctggggctac tacaaaccag cttcgcgcca
     2941 aaagatcatc gtcgttggga tgatatctgg gaagacggac tggaagctga aaagctacgg
     3001 gaagcattgc tgtcattaga acatgctttc cagtttgggc cagattcccc ttcatttata
     3061 caggatttcg aggcgctcac cggagataaa gttccggtcg cttcgctact gcctgagatt
     3121 cccggcgctc aaacaacgaa gtttaataaa gaccacttta tcaagcgtgg tgtaacggaa
     3181 tacctgtgcc cacattgctc tgcgttagct ctgttctccc tacagttgaa tgcgccgtca
     3241 ggtggcaaag gctaccgcac cggtttacgc ggcggtgggc cgatgaccac tctgattgaa
     3301 ttacaggaat atcagggtaa tcaacaaact cccttgtggc gcaaactgtg gatcaatgtc
     3361 atgccgcagg atgaagccga tttaccgcta cccaaaaaat ttgacgatct ggttttcccc
     3421 tggcttggcc cgacgcgcat cagcgaactg gccggtgcgg tggtaaccga tgagcaggtc
     3481 aataaactcc aggcgtactg gggaatgcct cgccgtattc gtattgattt taataccacc
     3541 acagtcggca actgcgatat ttgcggtgag cagagcgacg cgcttctgag cctgatgacg
     3601 accaaaaatt acggcgctaa ctatgccatg tggcagcatc ccttaacgcc ttaccgtgta
     3661 ctacttaaag agggcggcga gttttactcg gtcaaaccac aaccgggcgg tttaatctgg
     3721 cgcgactggt taggccttat cgaaacgggt aagtcagaaa acaatacgga acttcccgcg
     3781 ctggtggtga aactctttaa tgccagcagt ctcaagcagg caaaagtggg cctgtgggga
     3841 tttggctatg atttcgacaa catgaaagcg cgctgttggt acgaacatca tttcccgctg
     3901 ctgctcaata aaaagaaggc cagataccga aactgcggct ggctgcgcaa acggcttcac
     3961 ggattctaag tctgttacgg agtgcattga aagaagcatg gttctccgat ccaaaaggtg
     4021 caaggggtga ttttagtttt gtggatatcg acttctggaa caaaactcag catcgcttcc
     4081 tgaggttagt gcgccaaatt gaagaaggtc aggatgcgga tgaattactc agcaaatgga
     4141 ataaggaaat ttggttattc gcgcgtcagg attttgacga gcgtgtattc accaatcctt
     4201 atgagcccgt tgatttggaa cgcatcatga ccgcgcgcaa gaaatatttt acaacatcgg
     4261 cggagaagca aagtgctaaa gccgccaggg agaaaaagca ggaggctgct gaatgagtat
     4321 tgtaaaagag gagcacaaag ccacgctgcg caaatggcac gaggaattgc aggagaaacg
     4381 cggcgaacgt gccagtctgc ggcgtagtac gacggttaat gatgtttgtc tgacggatgg
     4441 atttcgcctg tttctgaaga acagacaaat aaagtggcag gatgaacctg agtggcgtat
     4501 caccgcactg gcgctgatag ccgcagttag cgccaatgtt aaagccattg atgaacggca
     4561 gccttttgcc gcgcaactgg cggcggtaat gtcaaagggg cgttttactc gcctgtcagc
     4621 agtaaaaaca ccggatgaat tactgcgcca gttacgccgc gccgtcagat tattaaacgg
     4681 ttccgtcaat cttgactccc tggcagatgg cattttccgc tggtgccagg agagcgacga
     4741 cctgctcaat catcaccgcc gccagcagcg gccaaccgaa tttatccgta tccgctgggc
     4801 gctggaatat taccaggctg gcgacgccga taacgaacaa aatcaataaa ggactatctc
     4861 atgacaacat ttattcagct tcatgtgtta accgcttacc ctgctgccaa ccttaaccgt
     4921 gatgacaccg gtgcgccaaa aactgtggtg ctgggtggag caacgcgtct gcgcgtttcc
     4981 tcgcaaagtc tgaaacgtgc gtggcgcact tctgcacttt ttgaacaggc actggcgggc
     5041 catattggta ttcgcagtgg gcgtattgcg cgtgaggcgg caactatcct gattgagaaa
     5101 gggattgaag ataaaaaagc catcgaatgg gcggcaaaaa ttgctgatta tcttgggaaa
     5161 gctaaaaatg acaaaaaacc aaaagatccg ctcactaacg ccgaaactga acaattagtt
     5221 catatcagcc cggcagaatt tgacgccgta aaagcgcttg ctcatcaact ggcagaagaa
     5281 aagcgcgcgc caaaagagga agatctcgcg ttgttacgta aagaccgtat agcggtagat
     5341 atcgctatgt ttggtcgtat gctggcgaat aaacctgagt ttaatgttga agccgcctgc
     5401 caggtcgcgc atgcgtttgg tgtcagtgaa acgattgtcg aagatgattt tttcaccgcc
     5461 gttgatgatt tgcgccaggc ctctgaagat gccggtgccg ggcatctcgg agaaaccgga
     5521 ttcggttcag cacttttcta cacctatatc tgcattgata aagatctgct ggtagaaaac
     5581 ctcggcggtg acgaagcgtt agctaatcag accatacgcg cttttacgga agccgcgctt
     5641 aaagtttccc caaccggtaa acaaaatagc tttgccagcc gtgcctatgc ttcctgggcg
     5701 ctggcagaaa aaggcaccga tcaaccacgc tctctggcgg cggctttcta tgaacccatt
     5761 aatggcacac gtcagttaga tgtggcggtg cagcgtatta caacgcttcg cgaaaacatg
     5821 aatacggtct acgaacagaa gaccgaatgc gcaagttttg acgtgatgaa caaacaggga
     5881 agcatgaagg acgtgctgga ctttatctgc gcgtaagggg cggcttatga gccaatatct
     5941 gatttttcag cttcatgggc cgatggcgtc ctggggtgtc gatgctccag gtgaagttcg
     6001 tcatacccat gaacttcctt cgcgctcagc attgctgggg ctactggctg ccggggtagg
     6061 gattcggcgt gatgataccg aacgcttaaa cgcgtttaat cgccactatt cactggtggt
     6121 ttgcgccagc cgtaacccgc gctgggcacg ggattatcac acggtccaga tgccaaaaga
     6181 ggtgcgtaaa gcacgttatt tcagccgtcg tgaagagttg agcgaccctg agcttttgag
     6241 cgcgattatc tcccggcgcg actactacac cgatgcctgg tggatggtgg cggtggcaac
     6301 aacagccgat gcgccttaca gcctcgaaca gttgcaggac ggtttacgcc atccagtttt
     6361 tccgctttat ctggggcgaa aaagtcatcc gctggcatta cctcttgcgc cgttactgct
     6421 cgaaggcaac gcgtgtgatg ccttatgtaa tgcataccaa cagtatcagg atcatttcca
     6481 taaactgaaa gtctcactcc cgaaacttca ggatgaatgc tggtgggaag ggaaacacga
     6541 cggcctggtc gcgagcaaaa tattacgccg ccgggatgtt cctttaaatc gtcagcagtg
     6601 gctgtttggg gaacgcaccg tcaatcaggg gccgtggctc agcaaggagg aaccatgtac
     6661 ctctcaagaa taaccttaca taccggccag ctttcgcctg cgcagttgct gcatctggtg
     6721 gatcgcggtg aatatgtgat gcatcagtgg ctgtgggatc ttttccctgg cggcaaagaa
     6781 aggcaatttc tttatcgtcg ggaagaactc
//
LOCUS       DAZOYL010000210         6746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZOYL010000210 DAZOYL010000000
VERSION     DAZOYL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.211"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(195..680)
                     /gene="sixA"
                     /locus_tag="ACHUOY_003071"
     CDS             complement(195..680)
                     /gene="sixA"
                     /locus_tag="ACHUOY_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR0683790.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(883..3027)
                     /gene="fadJ"
                     /locus_tag="ACHUOY_003072"
     CDS             complement(883..3027)
                     /gene="fadJ"
                     /locus_tag="ACHUOY_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR0683791.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3027..4337)
                     /gene="fadI"
                     /locus_tag="ACHUOY_003073"
     CDS             complement(3027..4337)
                     /gene="fadI"
                     /locus_tag="ACHUOY_003073"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR0683792.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4517..4801)
                     /locus_tag="ACHUOY_003074"
     CDS             complement(4517..4801)
                     /locus_tag="ACHUOY_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR0683793.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5173..6513
                     /gene="fadL"
                     /locus_tag="ACHUOY_003075"
     CDS             5173..6513
                     /gene="fadL"
                     /locus_tag="ACHUOY_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR0683794.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1674 a         1813 c         1614 g         1645 t
ORIGIN      
        1 acattattca tttcaacatg taactttggg caaaatcaag atacgcgcag aagaataatt
       61 tccgaatgaa agtagaaagt gttttattta tagatgggga gagtcttcga tagtagtgag
      121 attgacagat atccgctgcc tgatgagcaa ttatgcccaa tgaatctacc tcattgggca
      181 taatttggaa ctcatcagat agcttttgcc atcttcagat tacacgggct catctgccag
      241 ttgaatgtgc cgtttccgct ttcatcaagc gttacgctgg caatggccga ggtggtaaac
      301 ataggcggcg tttcgcccgg acataactcg gcaaccagat acccgactaa cggtaagtga
      361 gagatcacca gtaccgaagc aacaccttca ttggtcaacg cctgcaaata ggcactgacc
      421 agaccaacat cgccgcaggg cgttaactcc ggcagaactt ctgcactgga aggcaggttc
      481 agacaatccc ctacttcttc cagtgtttgc tcggctcgca ggaacgggct caccagaacg
      541 cgttcgattt ccactttttg acctttcagc cagttcgcca tcaggcgaga ttcgtcacaa
      601 ccattagtgg tcagaggacg aaccgaatca ctggcggcat caagggctgc gtcgccgtga
      661 cgcatgataa aaacttgcat attgcaccgc ttttgttaac cagattcgtc cgttttcttt
      721 catctgaaac cagagaaaga aaacggtggc cggcattgtg ccttatccat tcaccgaatg
      781 aaacgctgtt ttttacctca atggcgtaag tatagtcaat atgcgtttac attttgacca
      841 acactccgcc attcagcgcg gattcatata gctttgacct tcttattgaa ggtcagttgc
      901 agttgttttc caaaaacttt ccccacgcgc gcccatctcg accaaacgct cgcaaggggt
      961 aaaacgggaa ccatactgcg tggccagtcg ttgcattatt gcaaccactt cgcccgcgcc
     1021 gagagaatcg atatagcgga acggtccacc gagaaatggc ggaaaaccaa tgccaaatac
     1081 cgcgccaata tccccgtcac gcacgctacg gataacctgc tcatcaacac aacgtactgc
     1141 ttcattcagc atcaacatca cacaccgttc agcaacctgc ggtgcggaga gtcgcccctg
     1201 cccttgtgcg ccaatgagcg ggtaaatggc gggatcgacc tgttttttgc ttttacgccc
     1261 tttctgacca taaagataga aaccccgacc attttttctg cctttgcgat cgtcgttcaa
     1321 aattgaagaa acaacatttg caggcgcgct aaaacgttct ccataagcgg cttccagtac
     1381 aggaataatt ttagtcccgg tgtcgattcc tacctcatcc aaaagttgga ttgggcctac
     1441 cggaaaacca aatttcacta gcgcggcatc aatgtgctct acccgttcac cttcggtcag
     1501 catgcggata gcttcattaa tgtaaggcgc taagatgcga ttgacgtaaa acccggcttt
     1561 gtcacgcacg acaatgggcg ttttgccctg ttttttcgcc agttttaccg tagtggcgat
     1621 ggtttgcgcc gatgtacccg catgaggaat aatctctacc agcggcattt tttccacagg
     1681 actgaagaaa tgcaggccaa taacttgctc aggtcgcgcg gcgtgagcgg cgatatcacc
     1741 aatcggtaaa gatgacgtgt tcgaagcaaa aatggtatga gcggcgcaat tttgctccac
     1801 ttccgccacc atctgttgtt ttaattcgag attttcaaac accgcttcaa taatcagatc
     1861 gcgatgggca aagccgcgat agtccgtcgt tccggagatt aatgccagct gtttgtcacg
     1921 ttcgctggct ttgagatgac gacggcgaac tttgccctcc agctgatccc aactgtactt
     1981 cagcgcatga tttatgccct gcgggttgat atctttaatt ctgaccggaa gccccgcttt
     2041 acaagcagtg acataagcaa taccgccgcc catcaagcca ccacctaaaa ttcccacgct
     2101 gtttaatggc gcaggcggtg catcactacc gggatctttc ttcacgtccg tactggcaaa
     2161 aaagatacta cgcaaagctt gcgattgcgg cgtcatcgcc agttcgccaa aggcgcgagc
     2221 ttcagcgtca tagccgctgc tggtgccctg cgctaatccc gtttcaacaa cctccaggat
     2281 gcgttctgtc gccggataat tgccttgagt tttgtgttct gttttcttgc cgaccatttt
     2341 gaacagcagc gcacgaccta acggccccgc cagaatacgc tcgcgtacag gtagagggcg
     2401 ggaagatggg cgatccttct tcgccagctc aacagcggct tccagcaaaa tggagtgcgg
     2461 aacaacgtca tccaccagcc ccagctttaa tgcctgtttc gcccgaagtt gttttccggt
     2521 gagaatcatc tctaatgctg tactgacgcc tatcagacgc ggtaaacgct gggtgccgcc
     2581 tgaaccgggt aacaatccaa gttgtacttc aggcaaaccg agcaccgttt taggatcgtc
     2641 agtacaaacg cgaccgtggc acgccagcgc cagctccagc ccaccgccca ggcaagcgcc
     2701 atgaatagcc gcgataaccg gaatgggcaa agcatgaatc tccgccatca actgttggcc
     2761 ctgccgcgcc agtgcttccg cttcttgcgc cgttttgcag ttgccaatca tgttgatgtc
     2821 tgctccggcg ataaaattgt ccggtttcgc ggagataaac accacgccgc gcaactcttt
     2881 gttttcacgg agttgcttaa taatggcgcg cacctgcgag gcaaattccg ccttcagggt
     2941 attcattttc tcatccggta catcgatggt gatgatagca atgttgtcca gacgaacatt
     3001 aagagtaaac gctgatgcca tttccattat tccgcctcca gaaccattgc cgcgccaagc
     3061 ccaccggcag cacaggcggt aactaaacca aatccaccgc cgcgacggcg aagttcatgc
     3121 aatgtctggg taatcatccg cgcgccggtc gccgcgaagg gatgcccgta agcaatcgaa
     3181 ccgccaagca cgttaaattt gctatcgtcc acttcgccag tggcatgtgc acgccccagt
     3241 acttcacgag caaaacgttc actacccagc aactgaatat tcgccagtgt ctgagctgca
     3301 aaggcttcgt gcatatcgat caatgtcagt tcgctcatcg tcaaaccggc acgttccagc
     3361 gccagcggtg ttgaccaggc tggaccgagc aacatgtcct gccagacatc aatcgcagta
     3421 aatgcgtagc tgcgcagata ccccagcggc accagcccta attctttcgc ccgggattca
     3481 gtcatcagga tcaccgctgc cgcgccatcg gtcagcggcg tactgtttgc cgccgttacc
     3541 gttccgtgtt tgcgatcaaa cgccgggcgc agctttgcgt aatcggcaag cgaggaatta
     3601 ccgcgaatat tgttgtcttc gacaagcggt tgtttataag gagggataaa ggcagtcatc
     3661 acctcttctt tgagttttcc gtctgaccat gcctgagcgg cacgctgatg cgaacggtgc
     3721 gctaatgcat cttgctgttc tcgggtgatg ccgtaggttt tcgccatttg ttcggcggtg
     3781 tcgcccatcc gcaagccagt agaatattct gctaccgcag gcggtacggg cattaagtcg
     3841 cacaaacgca ggcgagagaa gagtttcagg cgctggctca tggttcgagc tttgttgaca
     3901 tcaaccagca cgcgcgccag ttttttactg acgccaattg gcaatacgga agaggaatct
     3961 gccccaccgg caatccccgc tcggatagtt cccgccatca ggctttctgc gacgtttgca
     4021 accgcctgga aactggtagc gcaagcgcgg ctgacgctgt aagcatcggt atgcacattc
     4081 attccggtac cgagaacaat ttcacgcgca atgttggggg cttcaggcat ttgtacgacc
     4141 tgaccaaaga ccagctgttc aatcacttca gcggggatct cgctgcgtgc cagcagttcg
     4201 cctaccacca tcttccctaa atcaaccgcg ggaatgccat gaaaagccgt cgcctggcgg
     4261 gcaaaaggcg tacgtaaacc gctaacaatg gcgatacgat cgccctggcg ggtaaccagc
     4321 ggtaaaacct gacccataac actcccctgt aaaaaaataa ataaagtggt ctgacctgat
     4381 catagtctta actatttttt tacatttagc caagcggaga aaaaggaaag tgggagctat
     4441 gacacagtga agacaggaga aagaaaagaa aacgcccctg ccagcaactg acaggggcga
     4501 acgtacccga gaggaattaa cgcagaccca gctggaagat cagcatttca gcttcgcagg
     4561 caaaagtgaa atcgatatcc aggcgcacac cgtcagactc ttcagtgaaa gtcggggaga
     4621 ttttgcacgg ttcggattcc acgctacggg ctttttcagt cagcgccgcc agcgtttgtt
     4681 ctgcttctgc gcggtttgca aacacgcggc tgtaagacgc ggtgcagtcg gagttgtcca
     4741 taatggtgcc aacatccata cagcagcaaa ccggggtttc atcagcacta catttactca
     4801 tcgttgattt cctctgtatg tgcacccaag gtgccagata aacgttgtgg atattttacg
     4861 cttccggaaa gtgctgctcc agttgttaat tctgcaaaat cggataagtg accgaaatca
     4921 cacttaaaaa tgatctaaaa caaaattcac ccgaatccgc gagtgcgcaa cctccaaatt
     4981 ttgccagctg gatcgcgttt cttagatcat atttgaaaaa agatggaaac atacttgcag
     5041 cattccagct ggtccgacct atactctcgc cactggtctg atttctaaga tgtacctcag
     5101 accctacact tcgcgctcct gttacagcac gtaacatagt ttgtataaaa ataaatcatt
     5161 gaggttatgg tcatgagcca gaaaaccctg tttacaaagt ctgctctcgc agtcgcagtg
     5221 gcacttatct ccacccaggc ctggtcggca ggctttcagt taaacgaatt ttcttcctct
     5281 ggcctgggcc gggcttattc aggggaaggc gcaattgccg atgatgcagg taacgttagc
     5341 cgtaaccccg cattgattac catgtttgac cgcccgacat tttctgcggg tgcggtttat
     5401 attgacccgg atgtgaatat cagcggaacg tctccgtctg gtcgtagcct gaaagccgat
     5461 aacatcgcgc ctacggcatg ggttccgaac atgcactttg ttgcaccgat taacgaccaa
     5521 tttggttggg gcgcttctat tacctctaac tatggtctgg caacagagtt taatgatact
     5581 tatgcaggcg gctctgtcgg gggtacaact gaccttgaaa ccatgaacct gaacttaagc
     5641 ggtgcgtatc gcttaaataa tgcatggagc tttggtcttg gtttcaacgc cgtctacgct
     5701 cgcgcgaaaa ttgaacgttt cgcaggcgat ctggggcagt tggttgctgg tcagattatg
     5761 caatctcctg ccgggcaaac tcctcaaggg caagcattgg cagctaccgc caacggtatc
     5821 gacagtaata ccaaaatcgc tcatctgaac ggtaaccagt ggggctttgg ctggaacgcc
     5881 ggaatcctgt atgaactgga taaaaataac cgctatgcac tgacctaccg ttctgaagtg
     5941 aaaattgact tcaaaggtaa ctacagcagc gatcttaatc gtgcgtttaa taactacggt
     6001 ttgccaattc ctaccgcgac aggtggcgca acgcaatcgg gttatctgac gctgaacctg
     6061 cctgaaatgt gggaagtgtc aggttataac cgtgttgatc cacagtgggc gattcactat
     6121 agcctggctt acaccagctg gagtcagttc cagcagctga aagcgacctc aaccagtggc
     6181 gacacgctgt tccagaaaca tgaaggcttt aaagatgctt accgcatcgc gttgggtacc
     6241 acttattact acgatgataa ctggaccttc cgtaccggta tcgcctttga tgacagccca
     6301 gttccggctc agaatcgttc tatctccatt ccggaccagg accgtttctg gctgagtgca
     6361 ggtacgactt acgcatttaa taaagatgct tcagtcgacg ttggtgtttc ttatatgcac
     6421 ggtcagagcg tgaaaattaa cgaaggccca taccagttcg agtctgaagg taaagcctgg
     6481 ctgttcggta ctaactttaa ctacgcgttc tgataacgcg ttcgcctgga taaagtcacc
     6541 tgcatagcag gtgactttaa ctcccccact tcaccgaaag tagtgtcctc attgcttacc
     6601 tcctgagttt tgcaaacagc ctgttggcag cttgcccttt caaatcaata agcggtatcc
     6661 acagacaaac ctgaagggaa aggcatattt tcaggcgttc tgctcgtcct tctcaaagag
     6721 tttacttttc tgcatttcca ggatac
//
LOCUS       DAZOYL010000211         6657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZOYL010000211 DAZOYL010000000
VERSION     DAZOYL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.212"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            17..153
                     /locus_tag="ACHUOY_003076"
     ncRNA           17..153
                     /ncRNA_class="other"
                     /locus_tag="ACHUOY_003076"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(244..930)
                     /locus_tag="ACHUOY_003077"
     CDS             complement(244..930)
                     /locus_tag="ACHUOY_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0683795.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHEWADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1511..2299)
                     /gene="phoH"
                     /locus_tag="ACHUOY_003078"
     CDS             complement(1511..2299)
                     /gene="phoH"
                     /locus_tag="ACHUOY_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR0683796.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2920..4191)
                     /gene="efeB"
                     /locus_tag="ACHUOY_003079"
     CDS             complement(2920..4191)
                     /gene="efeB"
                     /locus_tag="ACHUOY_003079"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR0683797.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(4197..5324)
                     /gene="efeO"
                     /locus_tag="ACHUOY_003080"
     CDS             complement(4197..5324)
                     /gene="efeO"
                     /locus_tag="ACHUOY_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR0683798.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5382..6212)
                     /gene="efeU"
                     /locus_tag="ACHUOY_003081"
     CDS             complement(5382..6212)
                     /gene="efeU"
                     /locus_tag="ACHUOY_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR0683799.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         1718 a         1781 c         1530 g         1628 t
ORIGIN      
        1 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
       61 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
      121 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
      181 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
      241 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
      301 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
      361 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg cccattcatg
      421 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
      481 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
      541 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
      601 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
      661 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
      721 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
      781 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
      841 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
      901 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
      961 atttcattac caatgtaact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     1021 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     1081 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     1141 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     1201 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     1261 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     1321 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     1381 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     1441 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     1501 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     1561 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     1621 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     1681 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     1741 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     1801 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     1861 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     1921 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     1981 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaagttt
     2041 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     2101 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     2161 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     2221 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     2281 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     2341 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     2401 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     2461 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     2521 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     2581 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     2641 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     2701 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     2761 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     2821 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     2881 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     2941 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     3001 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     3061 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     3121 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     3181 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     3241 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     3301 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     3361 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     3421 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     3481 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     3541 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     3601 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     3661 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     3721 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     3781 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     3841 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     3901 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     3961 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     4021 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     4081 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     4141 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     4201 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     4261 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     4321 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     4381 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     4441 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
     4501 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
     4561 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
     4621 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
     4681 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
     4741 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
     4801 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
     4861 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
     4921 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
     4981 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
     5041 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
     5101 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
     5161 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
     5221 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
     5281 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
     5341 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
     5401 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
     5461 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
     5521 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
     5581 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
     5641 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
     5701 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
     5761 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
     5821 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
     5881 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
     5941 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
     6001 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
     6061 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
     6121 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
     6181 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
     6241 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
     6301 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
     6361 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
     6421 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
     6481 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
     6541 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
     6601 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtc
//
LOCUS       DAZOYL010000212         6627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZOYL010000212 DAZOYL010000000
VERSION     DAZOYL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.213"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..146)
                     /locus_tag="ACHUOY_003082"
     CDS             complement(<1..146)
                     /locus_tag="ACHUOY_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683800.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSL"
     gene            224..1318
                     /gene="ddlA"
                     /locus_tag="ACHUOY_003083"
     CDS             224..1318
                     /gene="ddlA"
                     /locus_tag="ACHUOY_003083"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0683801.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(1342..1554)
                     /locus_tag="ACHUOY_003084"
     CDS             complement(1342..1554)
                     /locus_tag="ACHUOY_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR0683802.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1814..2122
                     /locus_tag="ACHUOY_003085"
     CDS             1814..2122
                     /locus_tag="ACHUOY_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR0683803.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2181..3275)
                     /gene="yaiW"
                     /locus_tag="ACHUOY_003086"
     CDS             complement(2181..3275)
                     /gene="yaiW"
                     /locus_tag="ACHUOY_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR0683804.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3288..4508)
                     /gene="sbmA"
                     /locus_tag="ACHUOY_003087"
     CDS             complement(3288..4508)
                     /gene="sbmA"
                     /locus_tag="ACHUOY_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR0683805.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4860..6017
                     /gene="ampH"
                     /locus_tag="ACHUOY_003088"
     CDS             4860..6017
                     /gene="ampH"
                     /locus_tag="ACHUOY_003088"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR0683806.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6018..>6627)
                     /gene="iprA"
                     /locus_tag="ACHUOY_003089"
     CDS             complement(6018..>6627)
                     /gene="iprA"
                     /locus_tag="ACHUOY_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR0683807.1"
                     /translation="KPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDTFVILE
                     GVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQTITLIE
                     QNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRIGVMNY
                     IHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         1678 a         1697 c         1604 g         1648 t
ORIGIN      
        1 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
       61 atttttgtga tctcgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
      121 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
      181 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
      241 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
      301 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
      361 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
      421 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
      481 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
      541 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
      601 gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg
      661 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
      721 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
      781 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
      841 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
      901 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
      961 gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     1021 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     1081 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     1141 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     1201 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     1261 gattgagctg gcgctgaagc gtcacgctgc ggataacgca ttgaaagcca caatgtaata
     1321 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     1381 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     1441 ccaacctttt gcttcaaatc ccagtaaccc caccacgcca ttaagaatga atataaggcc
     1501 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     1561 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     1621 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtaact cacaacgaag
     1681 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     1741 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     1801 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     1861 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     1921 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     1981 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     2041 cgggctgatc ccgtttttag taggctgcct catttttgca gtggtggcgc tatggctgca
     2101 ctggcgacat cgccgccagt aagatttacg ttgccagata cggcctaaaa cgccttatct
     2161 ggcatgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     2221 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     2281 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     2341 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     2401 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     2461 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     2521 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     2581 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca
     2641 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     2701 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     2761 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     2821 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     2881 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     2941 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     3001 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     3061 gtcggtgcca gcccctgact ttcaaaggtg atggcgagat ctttcgccca ggcatcgcgg
     3121 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     3181 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     3241 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     3301 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     3361 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     3421 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     3481 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     3541 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     3601 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     3661 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     3721 atccctacca ctgccagcca tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     3781 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     3841 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     3901 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     3961 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     4021 acatagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     4081 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     4141 agcgcggttt gaatcagatc atagaacggt gcataccagg cgttgacggc gaccccgact
     4201 tccaccaaaa accaggtgac gaagatgatc agtgcagtac cgagtattga ccagtattgc
     4261 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     4321 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     4381 ccggaagcgc cggtgatacg cgccacccag tctcccccac cggcttgcca gaagataacg
     4441 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgctc ccggctttgg gaaaaaagac
     4501 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     4561 atgaccacta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     4621 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     4681 acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga
     4741 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     4801 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     4861 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     4921 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     4981 atatttttta cggcagcggt gcaacgggga tggcgctggt tgttatcgac ggtaaccagc
     5041 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     5101 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     5161 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     5221 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     5281 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     5341 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     5401 cggcgtactc taaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca
     5461 agccttatac ccagttgttt gaagagcaga ttacccgtcc attggggatg aaagacacca
     5521 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     5581 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     5641 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     5701 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     5761 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     5821 gtccggggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     5881 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     5941 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     6001 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     6061 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     6121 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     6181 tcattccagt caatcattga taataatgtt gcgcggattt gttcgtagga attatgccca
     6241 atgagctgca cgtcgcgtaa ttccagaatt ctattttgcc aagctaacca gtaaaaagcg
     6301 tctcgccaga gttgattttg ttcaataagc gtaatggttt gtttggctgg tagatgatat
     6361 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     6421 gccagcccca taatataagg ggcctgggta ataccgataa gtacgttttc ttctctacgc
     6481 agagagataa ctccctctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     6541 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     6601 agcttaccaa attcctgaag aggttta
//
LOCUS       DAZOYL010000213         6565 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZOYL010000213 DAZOYL010000000
VERSION     DAZOYL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.214"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(104..1384)
                     /gene="hemL"
                     /locus_tag="ACHUOY_003090"
     CDS             complement(104..1384)
                     /gene="hemL"
                     /locus_tag="ACHUOY_003090"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR0683808.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            1550..1597
                     /gene="yadX"
                     /locus_tag="ACHUOY_003091"
     CDS             1550..1597
                     /gene="yadX"
                     /locus_tag="ACHUOY_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR0683809.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            1609..3030
                     /gene="clcA"
                     /locus_tag="ACHUOY_003092"
     CDS             1609..3030
                     /gene="clcA"
                     /locus_tag="ACHUOY_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR0683810.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            3054..3119
                     /gene="yadW"
                     /locus_tag="ACHUOY_003093"
     CDS             3054..3119
                     /gene="yadW"
                     /locus_tag="ACHUOY_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR0683811.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            3112..3456
                     /gene="erpA"
                     /locus_tag="ACHUOY_003094"
     CDS             3112..3456
                     /gene="erpA"
                     /locus_tag="ACHUOY_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR0683812.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3503..4126)
                     /locus_tag="ACHUOY_003095"
     CDS             complement(3503..4126)
                     /locus_tag="ACHUOY_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR0683813.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(4164..4964)
                     /gene="btuF"
                     /locus_tag="ACHUOY_003096"
     CDS             complement(4164..4964)
                     /gene="btuF"
                     /locus_tag="ACHUOY_003096"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR0683814.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(4957..5655)
                     /gene="mtnN"
                     /locus_tag="ACHUOY_003097"
     CDS             complement(4957..5655)
                     /gene="mtnN"
                     /locus_tag="ACHUOY_003097"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR0683815.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            5739..>6565
                     /gene="dgt"
                     /locus_tag="ACHUOY_003098"
     CDS             5739..>6565
                     /gene="dgt"
                     /locus_tag="ACHUOY_003098"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR0683816.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT         1539 a         1705 c         1748 g         1573 t
ORIGIN      
        1 cggatgcccg atgcgacgct ggcgcgtctt atcgggccta caaacggccc cgaatcgtag
       61 gtcggataag gcgttcacgc cgcatccgac aaaccatgct ggatcacaac ttcgcaaaca
      121 cccgacgtgc agcatcgatg gtgttattga tatcttccat gctgtgcgcc acggacataa
      181 agcccgcttc aaacgctgac ggtgccaggt aaacaccttc gtccagcatc atatggaaga
      241 aacgcttaaa gcgttccacg tcacaggcca tcacatcctg atagcacgtc acggactcgg
      301 cgtcggtaaa gaaaataccg aacatgccgc caacgtggtt aacgaccagc ggaattccgg
      361 cttcttctgc cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccaacgttt
      421 cgtgaacgcc cggttgcgcg acttcattca gacaggcgaa accggctgcc atcgcaattg
      481 ggttaccgga aagcgtaccc gcctgataga ccggacccgt cggggccagc gcatccatta
      541 catcacgacg accaccgaat gcgcctaccg gcattccacc gccgatgatt ttgcccaggc
      601 aggtgagatc cggttccaca ccgtaataat cctgtgcgcc agccagtgcc acgcggaagc
      661 cggtcattac ttcatcgatg atcagcaatg cgccaaattc gtcgcacagc gcacgcagac
      721 ctggcaggaa gtctggcagc ggtggaacac agttcatatt gcctgccacc ggctcgatga
      781 taatacaggc aatctcttgc gggtattgct caaacgcggc gcgtacagaa gccagatcgt
      841 tataagtaca ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct
      901 ggcctaacgt gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt
      961 aacacccttc aaatttaatg attttgtcgc gaccggtaaa accacgggcc agacggatgg
     1021 cgctcatcgt cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca
     1081 attcggtcac cagttgcgcc attttcactt ccatttcggt tggtgcacca aagcttaaac
     1141 cacgctcggc ggcttcaatc acggcattgc ggattgccgg atggttatgg cccagcacca
     1201 tcggccccca ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag
     1261 cgccgtccgc tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag
     1321 gggagttcac accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac
     1381 tcatggaggg ttcctgattc gtagaaaaag tgaatggctg ctattctatg ttattcataa
     1441 caagttaaat actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga
     1501 gtataaaagt tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc
     1561 gagtaaaatg ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat
     1621 actccctctt tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa
     1681 cttcttgagc gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg
     1741 cttgttgggc tggcagcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt
     1801 atgggggcgc tggtacatac tgctgataat tatccgcttc tgttaaccgt cgcttttctc
     1861 tgttcggcgg tgctggcgat gtttggctac tttttggtgc gcaaatacgc gccggaagca
     1921 ggtggttcgg ggatcccgga aattgaaggg gcgctggaag atcaacgtcc cgttcgctgg
     1981 tggcgtgtat tgccggtgaa gttctttggc gggctgggga cactcggcgg aggcatggtg
     2041 ttggggcgcg aagggccaac cgtgcagatc ggcggtaaca ttggccgtat ggtgcttgat
     2101 attttccgcc tgaaaggtga cgaagctcgc catacgctgc tggcaaccgg tgctgctgcg
     2161 gggctggctg cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg
     2221 cgtccgcagt ttcgctatac gttaatttcg ataaaagcgg tatttattgg cgtcattatg
     2281 tcgaccatta tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt
     2341 tctgatgcgc cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt
     2401 ttcggcccta tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac
     2461 ggcggcaata ttaccaaatg ggtactgatg ggcggtgcga ttggcggtct gtgtggattg
     2521 ctggggtttg tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc
     2581 gcggggaatt tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc
     2641 ttactctgct tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt
     2701 actgtgctgg gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctt
     2761 gaggcgggga cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg
     2821 ccgttaacgg ggatcattct ggttctggag atgaccgata actaccagct cattttgcca
     2881 atgattatta ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaaccgcta
     2941 tactcggcga ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc
     3001 aaggccgcat cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga
     3061 ttattattgg gttagaattt gcccaattgc cgatgtcgtt tggagcaaaa tatgagtgat
     3121 gacgtagcac tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc
     3181 gctgacgaag ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc
     3241 ggcttccagt atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa
     3301 aaacagggcg ttggcttggt ggttgatccg atgagcctgc aatatctggt cggcggttcc
     3361 gttgactata ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa
     3421 agcacctgcg gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa
     3481 ctatccggcg catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct
     3541 taagatgcca acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac
     3601 tggctgtttc cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga
     3661 taatacaggc agttgcgtag atttctgtac gtaaaatcat ggggatctcg cgggccagta
     3721 catcacgaat gatcccgccg ccaacgccag taatgacgac catacaaacc gcgattaacg
     3781 gaccgacttc cgcattaaag gctttattca cgccaatgcc gacaaacacc gccagaccaa
     3841 cggcgtccag caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga
     3901 tggtcagcat gctggttacc attgcaacga ccagatcggt gggatctttc acccaaaata
     3961 ccgggccgtg atccagcgcc atgtcgcgaa ttgtcccgcc gcctacagcg gtaaccacgc
     4021 ccagtaccag aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccggaga
     4081 tggcaaatac cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac
     4141 tgagcaccta aaaaggtcag gcgctaatct acctgtgaaa gcgcattaca gagctgttgt
     4201 gcagcgagga taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg
     4261 ggaattttga gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct
     4321 gtaatgacaa tcgcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag
     4381 ggaacccggc tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttttga
     4441 atcgactctt ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt
     4501 ttaggtttat cagcatattg cgctttcaat tgcgcgtact gatccagcag ggattgcgcg
     4561 gcttgttcgg ccttatccgg ttgcggactc cagggggcca attgacgtaa cgcattggca
     4621 atttgttcaa tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg
     4681 tcaacctgcc gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg
     4741 acaatgcgtt ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt
     4801 ggaggatagt cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt
     4861 tcagtgttgg cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgca
     4921 agaaaagaca gggcgaccag cgccctgaac agtgacttag ccatgtgcca gtttctgcac
     4981 cagtgactca accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct
     5041 aagatgagac tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt
     5101 gaaattgtgg cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg
     5161 gaagttgtgg cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac
     5221 aatcaggcca cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat
     5281 cagtttatcg tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc
     5341 aaatgccgtg acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac
     5401 tttcagcgtt ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca
     5461 gtgttccaac agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag
     5521 aagcgcaacc tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat
     5581 agtttgacgg ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat
     5641 gatgccgatt ttcatagatt tactcgcgat aagcccgatt tgaaagcata gtttaccatg
     5701 cgcttacggg gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa
     5761 aaaaaataaa ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga
     5821 tcctgcggat cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc
     5881 tgcaacaaaa gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta
     5941 cccactcgat ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc
     6001 tgaaagagct taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa
     6061 gcattgttga gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg
     6121 gcgaagcagc gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc
     6181 agcctctgac tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac
     6241 agcttaacga gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac
     6301 aaggcattcg tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg
     6361 gtattttaaa atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact
     6421 atttaatgaa aaagccgggt tattatcttt ctgaagaagc ctatattgcc cggttgcgta
     6481 aagaacttaa tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg
     6541 acgacatctc ctattgtgtg gcaga
//
LOCUS       DAZOYL010000214         6538 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZOYL010000214 DAZOYL010000000
VERSION     DAZOYL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.215"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUOY_003099"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUOY_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR0683817.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUOY_003100"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUOY_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR0683818.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHUOY_003101"
     CDS             2408..3157
                     /locus_tag="ACHUOY_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR0683819.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="ACHUOY_003102"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="ACHUOY_003102"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHUOY_003103"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHUOY_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR0683820.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="ACHUOY_003104"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="ACHUOY_003104"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR0683821.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="ACHUOY_003105"
     CDS             6018..6491
                     /locus_tag="ACHUOY_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR0683822.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1651 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgttac
//
LOCUS       DAZOYL010000215         6515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZOYL010000215 DAZOYL010000000
VERSION     DAZOYL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.216"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHUOY_003106"
     CDS             38..250
                     /locus_tag="ACHUOY_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683823.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHUOY_003107"
     CDS             283..501
                     /locus_tag="ACHUOY_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0683824.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHUOY_003108"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHUOY_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUOY_003109"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUOY_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR0683825.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHUOY_003110"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHUOY_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHUOY_003111"
     CDS             1917..2660
                     /locus_tag="ACHUOY_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR0683826.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHUOY_003112"
     CDS             2690..3229
                     /locus_tag="ACHUOY_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR0683827.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUOY_003113"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUOY_003113"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR0683828.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUOY_003114"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUOY_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR0683829.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHUOY_003115"
     CDS             4582..6102
                     /locus_tag="ACHUOY_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0683830.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6515)
                     /locus_tag="ACHUOY_003116"
     CDS             complement(6135..>6515)
                     /locus_tag="ACHUOY_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683831.1"
                     /translation="IANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKK
                     NTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQ
                     ASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1714 a         1489 c         1550 g         1762 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaat
//
LOCUS       DAZOYL010000216         6494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZOYL010000216 DAZOYL010000000
VERSION     DAZOYL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.217"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..492
                     /locus_tag="ACHUOY_003117"
                     /pseudo
     CDS             1..492
                     /locus_tag="ACHUOY_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(496..1887)
                     /gene="tcyP"
                     /locus_tag="ACHUOY_003118"
     CDS             complement(496..1887)
                     /gene="tcyP"
                     /locus_tag="ACHUOY_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR0683832.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(2020..2610)
                     /locus_tag="ACHUOY_003119"
     CDS             complement(2020..2610)
                     /locus_tag="ACHUOY_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0683833.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2773..3441)
                     /gene="hxpB"
                     /locus_tag="ACHUOY_003120"
     CDS             complement(2773..3441)
                     /gene="hxpB"
                     /locus_tag="ACHUOY_003120"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR0683834.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3588..4124
                     /locus_tag="ACHUOY_003121"
     CDS             3588..4124
                     /locus_tag="ACHUOY_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR0683835.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4165..5025)
                     /locus_tag="ACHUOY_003122"
     CDS             complement(4165..5025)
                     /locus_tag="ACHUOY_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR0683836.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5131..5421)
                     /gene="ghoS"
                     /locus_tag="ACHUOY_003123"
     CDS             complement(5131..5421)
                     /gene="ghoS"
                     /locus_tag="ACHUOY_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0683837.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5522..6451)
                     /gene="pfkB"
                     /locus_tag="ACHUOY_003124"
     CDS             complement(5522..6451)
                     /gene="pfkB"
                     /locus_tag="ACHUOY_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR0683838.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1694 a         1610 c         1579 g         1611 t
ORIGIN      
        1 ttaatgagaa ttttgttggg atggatttta cctgtgaatt ccgacccgaa aaaatgacga
       61 atttgatcaa tataaaaaat aggttggtta ttcattattt aaggactata tactatgaaa
      121 aaaattatat acatcctgcc gggagcattt ttgctgcgat tcaaaaaaaa tgagagtctc
      181 ctaaagtttc cttcgataat atcaatgctt aatatcaatc ttctttttaa tccactaaat
      241 cttccgggaa tgggaagtgg tgtgcttgaa gatattatga gtattccaga tagttcactt
      301 agaaaacgtc tcgggtatga ggttttaagc ttctcactac aggcacattc attgagccag
      361 gaatgtattg ataagttgga catttttttc gctgacgatc tgtttaaata tgagtcagta
      421 tgcatcgccg cgatggaaca tttaaaaagt aaagcaacgg cccctataca aaacggaccg
      481 ttgccagcat aagaattaac ggtgtgccag ttcggcgtcg tcttcgctat ccagaatggc
      541 tttatcggtt tgcttcagcc actggctggt cagcgtgcca gctgtcatcg agccactaac
      601 gtttagcgcc gtacggccca tgtcgataag cggttcaacg gagattaaca gcgccaccag
      661 ggttactggc aggcccatcg caggcagcac aatcagtgcg gcgaaagttg caccaccacc
      721 gacaccggca acgcctgcgg aactaacggt aacaataccg accagcgtcg caatccacat
      781 cgggtccagc gggttaatgc caaccgtagg cgcaaccatc accgccagca ttgccggata
      841 caaaccggcg cagccgttct gaccaatggt tgcaccgaaa gaggcggcga aactggcgat
      901 ggattcagga acgcccagac gacgcgtttg tgcttccaca ttcagtggga tagacgcagc
      961 actggaacgg ctggtaaagg caaacgtcag cacaggccat accttacgga agtacttcag
     1021 cggactcacg ccattaatgc ccagcagaat gccatgcact gcaaacataa tcagcagacc
     1081 gaggtaggac gcgacaacga aacttcccag tttgatgatg tcttgcaagt tagaacctgc
     1141 aaccactttg gtcattagag ccagaacgcc gtaaggggtc aactgcatga caaggcgaac
     1201 cagtttcatc acccagcttt gtagggtatc gatagcggct aagacgcgtt cacctttcgg
     1261 cgcatcatcc ttcagcagtt tcaatgcagc tacgccgagg aatgcggcaa aaattaccac
     1321 gctgataatt gacgtcggat tggctccggt aagatcggca aacgggtttt tcgggataaa
     1381 ggacaagacc agctgcggaa cgctcagatc agagacttta ccaacatagt tactttcaat
     1441 ggcgttcaga cgtgcagttt ctgcaccacc ctgaaccaga ccttcagccg tcaaaccaaa
     1501 caggttggtg accagcacac cgaccagcgc cgcaatcagc gtggtaaaca aaagcgtacc
     1561 gatggtcaga aaactgattt tgcctaactg agatgcgtta tgcagacggg caaccgcgct
     1621 cagaatagag gcgaagacta acggcataac gatcatttgc agcagttgaa catagccgtt
     1681 accaacgatg ttaaaccact gtacagaatc tttaagtacc tggctgtcag aaccataaat
     1741 ggtatgcagg gcaaggccaa aaaccacacc catcaccaga cccaccagca cttttttcgc
     1801 cagactccac tgtttatggc gggtctgagc cagcgcaaac agcagtacaa cgaacaccac
     1861 gatgttcgca attaatggaa agttcatccc cgttctcctg atcttattat ttctatcact
     1921 gcgtagcagt catagtgttg gcgcaagatt aacagaactg tgagctggct cttatattca
     1981 aatggaattg cttattccag aaaggtgatt tttgcctttt tagtattcaa cctgatgctt
     2041 aataagccga tgaaactgga tctgcaaggt attgatcatt tgcgatgacc aacgaacagc
     2101 gctgttctcg ggtaatgaat ggggcatcca taccgaccag acaaataatg ccatgcagat
     2161 aaaacgttcc agttggttgc ccttttgggg aaccaggata ggcaagcgga aacgccagcg
     2221 gcatggccag agcaggggaa caccggcggg tgtcagcata tcggcaagta tgtggctcaa
     2281 ataacccagc accattcctt gtagcgcatc agccggaatg aaccagcctt ccggaacctt
     2341 aaggtaaaag gttgccagca gcgcaaatac cgccagcaga ctgtgggtaa aaccacgatg
     2401 cccaaaagcg cgggcgatcg gttttgatat ccattttaat cgctgcccaa gaaacgactt
     2461 tgggtgatcg atgtccggta acaaacacgt caggattgcg gaagggacaa tatgccacca
     2521 gtcaccctgt gccagcacgg gcgtcagctc ggcatttttg gcaaataccg cacaagcaat
     2581 agaaaagaga aggtgacctt ccgccgtcat gataaaactc actaaactgt cgatacgtac
     2641 agtataggat ttttatacag tgcacggaag tggtgacggt gtaactcttt ctcttaaatt
     2701 gacgattaat ccgtcagttg aatgcaaatt cagataaaga accaggggca gcgtgatcac
     2761 tgcccctggt cattaaccga gaaggtcttt tgcggtgagt tctgtcagcg atgagagttt
     2821 gacgtttgct aatacaaaac gtggatcatt ttgcgcttct ggcgcaggaa cgacgatgga
     2881 acgcatgcgg gctgctttag aagcgatcat gccatttacc gagtcttcca gcgccacgca
     2941 ggtcagtggg tcaacgccca gttttgctgc gcagtcgaga tatacttgcg gatgcggctt
     3001 gctgtaaggc agtttttcag ccgaggcgag ggcatcgaaa ctgtcgcgta agtcaaacat
     3061 ggtcaacact ttttccagca tatgtagtgg tgacgcggag gccagtccca ccaataaacc
     3121 ttgttctttg cataacgcaa cagcttcgcg cacgcctggt aataatggac gtgtctcttc
     3181 aaccagtgaa atggcacggg caataacccg ttctactact tcctgacggc ttggcccatt
     3241 ccacggttgc cgggcgtacc aaagatcgac caccatatcg atgcgtaaac ctaaggtgtc
     3301 cggcagctcg ttacgacggg agatatccac ccccaggctt gccatcacat ccagttcggc
     3361 tcgatcccgt aaaggttctg agtcgataag taatccatcc atatcaaaaa ttgcagcaag
     3421 aatctgacgc ggggttgaca ttacaacctc tcctttagag cattatgtgt aaggtattaa
     3481 gccgtgctta tgattaagcg cctatcatat cgcctttaaa gaatgggcga aaacagatat
     3541 ctacaggtaa actaagtgaa acgacgcgtc ttaatcaagg agaactcatg acgtatcaac
     3601 aagctggacg cattgctgtt ttgaaaagga ttttgggttg ggtgattttt atacctgcat
     3661 tgatctccac attgatttct ttgctgaaat ttatgaatac ccggcaggaa aaccaggaag
     3721 gcattaatgc ggtcatgctc gactttactc atgtcatgat cgatatgatg caggcgaata
     3781 cgcctttttt gaatctgttc tggtataact ccccgacccc taattttaat ggtggcgtga
     3841 acgttatgtt ctgggtgatt ttcatcctga tttttgtcgg actggcgttg caggattccg
     3901 gtgcccggat gagtcgccag gcgcgcttct tgcgggaagg tgttgaagat caactcattc
     3961 tggaaaaagc caaaggggaa gaggggctaa cgcgtgaaca aattgagtcc cgtattgttg
     4021 ttccgcacca tactattttc ctgcagtttt tctcactata tatcctgccg gttatttgta
     4081 ttgctgcagg ttatgtgttc ttttctctgc ttgggtttat ttaatcaaaa ggccgcacgg
     4141 agcggccatt atcctcaacc catatcatgc tgctaataat ctatccaatg actgctgagc
     4201 aataaccaaa tgctgaccgc caaataatct tgcacgattt aacagcgtgt agagttggta
     4261 aaccggttga cgttcaagga aatcggcagg tagcggggat actgactgat agccgtcata
     4321 gatttgtggc ggctgttcag tatgcagcgg taacatcgcc aggtcgcact ctcggtcacc
     4381 ccagtagcag gcagggtcga aaatgtacgg gccatccgga cccagcgcac agttgccgga
     4441 ccataaatcg ccgtgcaaca gagaaggctg cagttgatgc gaggccagac gctgctgaat
     4501 atgctcgacg atggcgtcga tattgccgaa agcgatccct ttctctgctg ccagttccaa
     4561 ctgccagcca atccgttgtt cagcaaaaaa cgttgaccag cgacgttgcc aggtgttggg
     4621 ctgtggagtt gtggagagcg agttatcaaa atcgaggcca aattgtggtt ggtcactcca
     4681 ttgatgtaaa cgcgcaattt gctgaccaag aataaatgcg ctatgcgcat ccagcggacg
     4741 aggtgggaga taatccatca ccagaaaact gtagtcacgg tcagcgccta ctgcccaaac
     4801 cttaggcacg gtgacggttt tactacgcga cagtaactcc agttggtcgg cttcggcggt
     4861 aaaaccggga agcagttccc tttcatcaca tttgacgaaa aagtcatgtc ctgcatagcg
     4921 caaatgccat gcggcatgga cttctccgcc aggcagttca ttacgcagtt cgatttcgcc
     4981 ttcacctaac tgctcgctca aaagacgact gattgcctgc cacatgatgt ctctcccatg
     5041 ttgtctgcca gatcataagg ttagcgcaag aatgcggtgt aaaaacacga gctaacgcac
     5101 accacagact gattattgcc caaaaaccat ttattttctg ttgctattcc attcctccca
     5161 agtcgtgatg gtgatttcag gatctttgcc ggttgcactt tgggtaagcc ccgaaaccag
     5221 ttctctaatt tcttcttcac tttgggtact gataagtcca aaagtgttag tccccagctc
     5281 atggatattt ccctcatcat ccgtcatggt gagcaaaaaa ccgtcgcgag tcaggtaatt
     5341 attcaactcg ttaatttcag tcaatgtatc ctcatgcaac atgacggtta tgacataacg
     5401 gacaagatcg ccactggcca tagttcaccc ctttgttatc acatgacttt tgagcaaagt
     5461 cggagaaacg cgttgccgac aggttggtgg tgattccccc aatgctgggg gaatgttttt
     5521 gttagcggga aaggtaagcg taaatttttt gcgtatcgtc atgggagcac agacgtgttc
     5581 cctgattgag tgtggctgca ctccccgcag ctacgccaaa acggaccatc tcttcaagag
     5641 aggcattttc tgccagtttc agtgtcatcg cgccgaccat gctgtcacca gcgccaacgg
     5701 tactctggct tttcaccggt ggtggcacca cctgaatgca gttttcacta tcaacaccca
     5761 gcgctccttg tggaccaagg gaaacgacaa cccgtttggc cttgccgcta ttaacgattt
     5821 cctgcgcggc tttgcggaca tcgtccggct gggtgagttc gcgattcacc agcgcactga
     5881 gttctttttg gttaggctta accaactcga tgttaccaat tgccagtgct gcacttaacg
     5941 cttcgccaga actgtcgacg atgcagcgga tcccttgttt ttgcgaagcg gaaatcagtt
     6001 gggttaattt ttccagcttc acacctggcg gcaggcttcc gcttatgacc aggatggccc
     6061 cggattcaat ttccagaact tgctcttcaa gctggcgaaa ctcatcttca tttaatgccg
     6121 cgcctggcat aacaaaacga tactgctcac cgcttgcttc cacatgtaca tgtaaattct
     6181 gccgggtcca gtctttggct tctacagtag cgacggggac attttcatcc gccaacagtg
     6241 aaaccaggtg ttcgccggtc gcgccacccg ccgggaagat cgctgtggca ctgcctccaa
     6301 gatgggcaat ggcgcgggcg acgttgatgc cgccgccccc gggttcgaac accggtgcgg
     6361 tacagcgcag ttttccttcg ggataaattt gcggggtaat tgttgcgcta tcgagagagg
     6421 gcgcaagtgt caacgtatag atacgtacca tcatttcctc ctataggctg atttcagtct
     6481 ggcaccgaat cagc
//
LOCUS       DAZOYL010000217         6480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZOYL010000217 DAZOYL010000000
VERSION     DAZOYL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.218"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..153
                     /gene="ymgJ"
                     /locus_tag="ACHUOY_003125"
     CDS             <1..153
                     /gene="ymgJ"
                     /locus_tag="ACHUOY_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR0683839.1"
                     /translation="LVILFCRRPHCRKISVRQYYLKRVIFYPTTLQRTAFKKCLFLMR
                     DIIISS"
     gene            114..233
                     /locus_tag="ACHUOY_003126"
     CDS             114..233
                     /locus_tag="ACHUOY_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0683840.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(712..978)
                     /gene="minE"
                     /locus_tag="ACHUOY_003127"
     CDS             complement(712..978)
                     /gene="minE"
                     /locus_tag="ACHUOY_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR0683841.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(982..1794)
                     /gene="minD"
                     /locus_tag="ACHUOY_003128"
     CDS             complement(982..1794)
                     /gene="minD"
                     /locus_tag="ACHUOY_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR0683842.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1818..2513)
                     /gene="minC"
                     /locus_tag="ACHUOY_003129"
     CDS             complement(1818..2513)
                     /gene="minC"
                     /locus_tag="ACHUOY_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR0683843.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3033..3401
                     /locus_tag="ACHUOY_003130"
     CDS             3033..3401
                     /locus_tag="ACHUOY_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0683844.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3509..3910)
                     /gene="pliG"
                     /locus_tag="ACHUOY_003131"
     CDS             complement(3509..3910)
                     /gene="pliG"
                     /locus_tag="ACHUOY_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0683845.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4152..4445
                     /locus_tag="ACHUOY_003132"
     CDS             4152..4445
                     /locus_tag="ACHUOY_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR0683846.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4517..5176
                     /locus_tag="ACHUOY_003133"
     CDS             4517..5176
                     /locus_tag="ACHUOY_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR0683847.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5268..5714
                     /locus_tag="ACHUOY_003134"
     CDS             5268..5714
                     /locus_tag="ACHUOY_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR0683848.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(5921..>6480)
                     /gene="hlyE"
                     /locus_tag="ACHUOY_003135"
     CDS             complement(5921..>6480)
                     /gene="hlyE"
                     /locus_tag="ACHUOY_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR0683849.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1655 a         1447 c         1535 g         1843 t
ORIGIN      
        1 ttagtcattt tattttgccg acggcctcat tgtcgaaaga taagcgtacg acagtattat
       61 ctgaaaagag tgatttttta tccaactaca cttcagcgca ctgcgtttaa aaaatgcctc
      121 tttcttatgc gggatatcat catttcatca tgatgtcttt ggtgagcggt gaacacaata
      181 cacctgcgct gtctcttcag gatgaatccc ctggtctggt gcctgcgggc tgatgttgca
      241 gcagagctta ggtcacttag acgctactat catttatcca atggcatgga atcgaaatca
      301 gtcgataccc gcagtatata tcgtgaactg gtcgttctga ccatcaacga ttaaactgcg
      361 cttcggcgca gttttcgttt acagggggct aaaagggaaa attctggggc aaaaaaagcc
      421 cgccagttac ggcgggaaac ctcatcctat gggagaacaa tgaataatga aattgcgggg
      481 ttatcatccc ccagtgtatc cacactaaca ataaggttat ttactcagcc aggcataaac
      541 attatgttct gtgcgtggga acagccttaa ggtgtaaagg gggaggtgga aatagcaatg
      601 aggagtatca gcaagaatac tcgccgcttt accacaacgt ggatgagagg gatgaaaaac
      661 tcaaggcaga aatgactctg ccttgaagat aaatgcgttt ttacagcggg cttatttcag
      721 ctcttctgct tccggtaagg tcacgttcag ctcaagaata gaaatatcgc catctttttg
      781 ctcaagctgt acggttacca tctcaggatc aatttgtacg tatttacaaa tgacctcaag
      841 aatatcttta cgcaactgcg gcagataatg cggttctgca tcgctgcgac ggcgttcagc
      901 aacaataatc tgcagccgtt cttttgcaat gttggctgtg tttttcttcc gcgagagaaa
      961 gaaatcgagt aatgccataa cttatcctcc gaacaagcgt ttgaggaagc ctttcttctc
     1021 ttcttcaatg aagcggaaag gacgttcttc tcccaacaga cgttctacgg tatctgcgta
     1081 ggctttaccc gcatcggcgt taatgtcgag aatgaccggt tcaccctggt tagaggcgcg
     1141 taatactgat tgatcctctg ggatcacgcc gacgagtttg atgcgcagga tctccagcac
     1201 atcttccatg ctcagcatgt cacctctgct tacgcggcct gggttatagc gcgttaacag
     1261 caggtgctct ttaataggct cttcgccatt ttctgcgcgg cgtgatttcg acgccagaat
     1321 gcctaaaata cggtcagagt cgcgtactga ggagacttcc gggttggtgg taataatggc
     1381 ttcgtctgca aaatagagtg ccattaacgc accggtttca atccctgccg gggagtcaca
     1441 aacgataaat tcaaaatcca tcgctttcag atcatcaaga actttggcga ccccttcacg
     1501 ggtgagggca tctttatcgc gtgtttgcga tgccggaaga atatagagat tttcagtacg
     1561 cttatcttta attaacgcct gatttagcgt tgcatcgccc tgaatgacgt tgacgaaatc
     1621 gtaaacgacc cggcgttcgc aacccataat caggtcgaga ttacgcaggc cgatatcaaa
     1681 atctatcacg acagttttct ttcccttctg ggccaaacca gtggcgatgg ccgcgctgga
     1741 ggttgtctta ccaacccccc ctttgcccga agtaacaaca ataatgcgtg ccatagaaat
     1801 tccttgttaa aaagggatca atttaacggt tgaacggtca aagcgttttc gactaactgc
     1861 agtcgcgccg ctttgccata aaattctgct gggatttgat cactcagcca gtattcacct
     1921 gcgatggaca ccaattccgc catcaggttc gtacaaaata tttgcgtttc ccggtcacca
     1981 cttgcccctg ccagcgcacg accgcgcatc atgccataga catgaatgtt cccatcggca
     2041 atcaattcgg ccccagcgct aacgtggctt gtaacaatca gatcacattg tggagcataa
     2101 atacgctgac cggaacgcac cggggtatct attaaacgcg tttttgtgac cggcgtggta
     2161 ttttgcgctg gagcctgtgg tgcgggagct ggacgtggcg ctttttcctt tccttccgtc
     2221 aggataggca gccccatttt ttcaatttcg gctttaagtt gcgcgtcttt gcagccactt
     2281 acgccaataa cccgcaaacc ggttgccgaa accgccttat gcatcgctga ccagtttacc
     2341 gggtcttcca gtgcactgac gttgagtaca acgggggcat gttttaaaaa tgcgggggcc
     2401 tgagcgattt tgtcttccag cgcctgatgg ataaccttag gttctgcctc atgcagatga
     2461 accacagata aagtgaagct actgccttta agctcgattg gcgtgtttga catcctggcc
     2521 ttactcaatt agctattaat catcgccagc gcgcgatgat gttccgaaga ctataaggca
     2581 tgttatagtc tggattatat tgaggcaagt caccctccca tttattcaga gtaaaagtct
     2641 attctgtgat aaatggcgct gattcatagc ttaaaaaata cccttgtcaa tcaacccatt
     2701 gccgccgtac ttttgattct tcttatttac gcttcctttt tccgcaccct aatcgtgaca
     2761 gcatctccag acctgtttaa atatccagca ttgaggcaag tgtatttttt caccacgcca
     2821 ggctttctta actttccatt ttttgcagca tcattaaaga acagataccc gttatcttaa
     2881 tatccgccac agaattgctc tatcatagaa accgcagaag acgtctgata cttctgtcag
     2941 gtcatcgtaa ctcgtccagg tcagcggttt tgattacgga ttgatgacga aatgagcata
     3001 agtgttgtac ggttggatta tcaggatgtg ctatgaatat gatgcgtatt atttatatcg
     3061 gattgtcagg tgtggggatg atgttctcat caatggcgtc tggacatgac gctggcggac
     3121 ttcaatctcc ggcgtgcggc gttgtttgcg acccgtatat ttgtgtgaac tcagatggca
     3181 tttccccaga gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac ttacaatcat
     3241 tacaaggcta cgatcccagc gaatttacat tcgctaacgg tgtattttgc gatgttaaag
     3301 aaaaattatg tcgtgatgat cgttattttg gtgtagatgg taagcggagt ggaaaaatca
     3361 atcaaaccac cacaaagatg ttatttatgt gtcgtgagtg atgccttgtt tacattatgt
     3421 tctctgctat tcgtcattaa cctcttgagc gacagagagg agtggggcgt taacgtctat
     3481 gatggggata gtccccgtcg gttgacattt acttaatttg aatatcgacg ttatattttt
     3541 tagttttgtt tttacgggcg tcgtttcgag tttgcagcac tcgcagctca tatttgccag
     3601 aagcagggag tgagtattgt ccgtgactat cgagttctgg ggaatatctg gataaatcaa
     3661 cagaatcatc gattcctggc ccgaagagat aagtatcagc gccttcatta gagatactca
     3721 catgcacttt ctgacctttt ttggcataga aggtatatgt atcgtaatcg tatcccttta
     3781 tttcgccgga gtactgagcg ctgctatggc ctttgctgaa ctcaacattg acatttttac
     3841 ccgcagcaaa gctcgtggag gttagcagtg caagcagcaa tacagcctta ctgatgctct
     3901 tgattttcat tggtagtctc ctgttcagaa tgagtacgat gaactgttat aatataacaa
     3961 tcccttacgg gagaattcca gtggcttttc tgcgttcggt aaaatcccgg cgcggcattt
     4021 tttgccgggg ggattactta taaaggggca tggactcaac aaaccgcaga aatctacagc
     4081 tggttggctt gtggcgcggg tttcattgat gttagcttat gccaaaacct ggcatcttaa
     4141 agagtaaaag tatgttttgt gtgatttatc gaagcagcaa gcgtgaccag acctatttat
     4201 atgtcgaaaa aaaagacgat ttttcgcgtg ttcctgagga actgatgaaa ggttttggtc
     4261 agcctcagtt agcgatgatt ctgccgttgg atgggcggaa gaaactggtc aatgctgata
     4321 ttgaaaaggt taaactggca ttaaccgagc aaggttacta tttgcagtta ccgccaccac
     4381 ctgaagattt gctgaagcaa catctttccg tcatggggca gaaaacagac gacactaaca
     4441 aataaccgat atccggcggt ggcattatct ttgtcggcgc gggttttcat atccacgata
     4501 aggtgagggg aacgttatgt atcaacatca caactggcaa ggtgcgctgc tggattatcc
     4561 ggtgagtaaa gtagtctgtg ttggcagtaa ctatgccaaa catattaaag agatgggcag
     4621 cgcagtgccc gaagagccag tgctgtttat taaaccagaa acggcactgt gcgatctgcg
     4681 gcagccattg gcgatcccat ccgatttcgg ttcagttcat catgaagtcg aactggcggt
     4741 gttgattggc gcgacgctgc gtcaggcaac ggaagagcat gtccgcaaag ccattgccgg
     4801 ttacggcgtg gcgcttgatc tgacattgcg tgatgttcag ggaaaaatga agaaagccgg
     4861 gcagccgtgg gaaaaggcta aagcgtttga taactcttgt ccgctttccg ggtttatctc
     4921 cgcggcggaa tttaccggcg atccacaaaa tacaacgctg ggcctgagcg taaacggcga
     4981 acaacgccag caaggtacga ctgcggacat gatccataaa atcgttccgt tgatcgctta
     5041 tatgagcaag ttttttaccc tcaaggccgg tgacgttgtg ttgacaggca cgcctgatgg
     5101 cgtcggcccg ttgcaaagcg gtgatgagct gacagtcact ttcgatgggc attctttgac
     5161 aactcgcgtt ttgtaatact ttttgccgcc tgaaagcggc ggcaaaactt gcatcgctgt
     5221 gccagactgg ttataaggtg cgttttaacg taatggcgga acacctgatg agcgatgtac
     5281 ctttctggca aagtaaaacc ctggacgaaa tgagcgatgc ggaatgggag tcgttgtgtg
     5341 atggttgcgg tcagtgttgc ctgcataaac tgatggatga agacaccgac gaaatctact
     5401 tcactaacgt cgcctgtcgc cagctcaata ttaaaacttg tcaatgtcgg aactacgaac
     5461 gtcgtttcga gtttgaaccc gactgcatta aattaacccg tgaaaatctg ccaacattcg
     5521 aatggctgcc aatgatctgc gcttatcgtt tgctggcgga aggtaaagat ttacctgcgt
     5581 ggcatccgct acttactggt tcgaaagcgg caatgcatgg tgaacgtatc tctgtgcgtc
     5641 atatcgcagt gaaagaatca gaagtgattg actggcagga tcatatctta aataaacctg
     5701 actgggcgca gtgataaatt taatagatgt tgatgggggc tttcttaata tacgtctgga
     5761 tgctcgccat ttcgtagccc tgtttttgaa agcgctctgt cataatgaga gttacccgat
     5821 accttattat caacaggtaa ctctcaatct ttaaaaaacg tctgtgtttt taaatgtgat
     5881 aaaccttata ccttgagtac atggagagaa taatcgctga tcagacttca ggtacctcaa
     5941 agagtgtctt tttaccgtgt tttttctgat actcattaca ggtgttaatc atttttttgg
     6001 ccgcttcttt tagcaaagaa agcattaaat catcataatc aacgtagaat ctggtggttt
     6061 cagtttccgt ttttatctct ccgatggcgg ctatttcggt ggttaatttc aatttggcgg
     6121 catcgatatc tttattcgct tgtttaaccg tgttagacag ggtggtaaag aaactctgca
     6181 cagattttaa cttgttcttc aattctggga tcagtttccc ttcaactacg cccgcagcaa
     6241 tagaatagga aatgattaaa ccaaatggac cggcgacgac accggctgcg gcaccggcat
     6301 acgcttcctt cctgatttta tctacctgtg actggaaata gctgcttttt tctgaaaaat
     6361 cattggttaa ctggctatct aacgccagca gtttcccgga agcgttgttg aaactttgtg
     6421 agcttaccag cagggatttt tgcgcttcat tcagcttcgt gatgccgtca tccagtacct
//
LOCUS       DAZOYL010000218         6473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZOYL010000218 DAZOYL010000000
VERSION     DAZOYL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.219"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            115..357
                     /gene="yjbE"
                     /locus_tag="ACHUOY_003136"
     CDS             115..357
                     /gene="yjbE"
                     /locus_tag="ACHUOY_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR0683850.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            471..1109
                     /locus_tag="ACHUOY_003137"
     CDS             471..1109
                     /locus_tag="ACHUOY_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR0683851.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1106..1843
                     /locus_tag="ACHUOY_003138"
     CDS             1106..1843
                     /locus_tag="ACHUOY_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0683852.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1843..3939
                     /locus_tag="ACHUOY_003139"
     CDS             1843..3939
                     /locus_tag="ACHUOY_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0683853.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQESHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3986..4264)
                     /locus_tag="ACHUOY_003140"
     CDS             complement(3986..4264)
                     /locus_tag="ACHUOY_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683854.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQSDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4265..4408
                     /locus_tag="ACHUOY_003141"
     CDS             4265..4408
                     /locus_tag="ACHUOY_003141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683855.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            4534..4944
                     /gene="psiE"
                     /locus_tag="ACHUOY_003142"
     CDS             4534..4944
                     /gene="psiE"
                     /locus_tag="ACHUOY_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR0683856.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4988..6463)
                     /gene="xylE"
                     /locus_tag="ACHUOY_003143"
     CDS             complement(4988..6463)
                     /gene="xylE"
                     /locus_tag="ACHUOY_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR0683857.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1608 a         1658 c         1748 g         1459 t
ORIGIN      
        1 aaacaccatc gctaattccc cgcgccgtaa ttcgcatgct ttagttgtgt atactcgatc
       61 ccgcccgaaa tgtttttggg taaatctcca ttcattcaat gaagggaaat tgttatgaaa
      121 aaagttctgt atggcatttt tgccatatct gcgcttgcgg cgacttctgt gtgggctgca
      181 cctgtacagg tgggcgaagc ggcaggggcg gcagcaacgt cggtttcggc ggggagttcc
      241 tccgcgacca gcgtcagcac cgtaagctcg gcggtgggtg tcgcgctcgc ggcaaccggt
      301 ggcggtgatg gttctaatac cgggaccaca acaaccacaa ccaccagtac ccagtaataa
      361 agtatgtatc cccaaaataa ttcgagtcat tgcatctgtg gcttgaaata tgaagggatt
      421 aaccataacc acactccggt gtggttattc tgcccctctg gagaagagtc gtgaagcgac
      481 ctgcactcat tcttatctgc ctgttattac aggcctgttc agccacgact aaagagctgg
      541 gcaattcact gtgggacagt ctgttcggca cgccaggcgt acagctgacg gacgatgata
      601 ttcaaaatat gccctacgcc agccagtaca tgcagcttaa tggcgggccg caattgtttg
      661 tggtgctggc cttcgctgaa gacggacaac aaaaatgggt cactcaggat caagccactc
      721 ttgtcacaca acatggtcgt ctggtgaaga ctttgcttgg cggcgacaac ctgattgaag
      781 tgaataacct tgccgccgac ccgctgatta aacccgcaca aattgttgat ggtgcaacat
      841 ggacgcgcac gatgggctgg accgagtacc agcaggtacg ctacgccacc gcacgctcag
      901 tcttcaaatg ggatggcacc gataccgtca aggtcggcag cgatgaaacc ctggttcgcg
      961 tgctggacga agaagtctcc accgaccagg cgcgctggca taaccgctac tggatcgaca
     1021 gcgaagggca aattcgccag tcggaacagt atctcggcgc ggattatttc ccggtgaaaa
     1081 ccactctcat caaggcggca aaacaatgat taaacaaact attgtcgcgt tgcttttgag
     1141 tgtgggagcg tcatcggtct tcgcggcagg aaccgtcaag gtgttcagca atggcagcag
     1201 tgaggccaaa acgctaacgg gcgcagagca tttaatcgat ctggtagggc aacctcgact
     1261 ggcaaacagc tggtggcctg gtgcggtgat tagcgaagag ctggcaacgg cggcagcatt
     1321 gcgtcagcag caggcgttgc tgacacggct ggcagaacag ggcgcagatt ccagcaccga
     1381 cgatgccgcc gccattaacg ccttacgcca gcaaatccag gcattggaag tgacaggcag
     1441 gcaaaaaatc aatcttgatc ccgatatcgt ccgcgttgcc gaacgcggta acccgccgtt
     1501 gcagggcaac tacacgctgt gggtcggacc accgccgtcc acggtcacgt tgttcgggct
     1561 tatcagccat cctggcaatc agccattcac tccaggtcgc gacgtggcga gctatctctc
     1621 tgaccagagc ctgctcagcg gtgcggatcg cagctacgcg tgggtcgttt acccggacgg
     1681 acgcacgcaa aaagcgccgg tggcttactg gaacaagcgt cacgtagagc cgatgcccgg
     1741 cagcattatt tatgttggcc tcgcagactc cgtctggagt gagacgcctg atgcccttaa
     1801 cgccgacatt cttcagactc tgacgcagcg gataccccaa taatgaaaaa aagacatctg
     1861 cttagcttac tggcgctggg cattagcaca gcttgctacg gcgaaacata tcctgcgccc
     1921 attggtccgt cgcagtcgga tttcggtggc gtaggattat tacaaactcc caccgcgcgt
     1981 atggcacggg aaggggagtt gagtctgaac tatcgcgata acgatcagta ccgttattac
     2041 tcagcttcag tgcaactctt cccgtggctg gaaacaacgc tgcgttacac cgacgtgcgc
     2101 acccggcagt acagcagcgt cgaagcgttc tctggcgatc aaacgtataa agataaagcc
     2161 ttcgatctca aactgcgttt gtgggaagag agttactggc tgccgcaagt ggcggttggt
     2221 gcgcgggata tcggcggtac ggggctgttt gatgcggaat atcttgttgc cagcaaagcc
     2281 tgggggccgt tcgattttac gctcggcctg ggctggggat atctgggcac cagcggtaat
     2341 gtgaaaaatc cgctctgttc agccagtgat aaatattgct atcgcgataa cagctacaaa
     2401 caagcgggat ctatcgacgg cagtcagatg ttccacggcc cggcctcact gtttggcggc
     2461 gtggaatacc agacgccctg gcaaccgctg cgcctgaaac tggagtatga aggtaataat
     2521 tatcagcagg attttgccgg gaagctggag caaaaaagta agtttaacgt cggggcgatt
     2581 tatcgcgtta ccgattgggc cgacgttaac cttagctatg aacgtggcaa cacctttatg
     2641 tttggcgtca cgctgcgcac caactttaac gatctgcgcc cgtcctacat cgataacgcc
     2701 cgcccgcaat atcaaccgca gccgcaggat gccattttgc agcactcggt ggtggcgaat
     2761 cagttaacgt tgctgaaata caatgccgga cttgccgatc cgcagatcca ggcgaaaggc
     2821 gatacgctgt atgttaccgg cgagcaggtg aaatatcgtg attcgcgcga agggatcatc
     2881 cgtgctaatc ggatcgtgat gaacgatctg ccggatggga tcaaaacgat ccgcattacg
     2941 gaaaatcgcc ttaacatgcc gcaggtgacg acggaaaccg atgtcgccag cctgaaaaat
     3001 catctcgccg gagagccgtt gggccacgaa acgacgctgg cgcaaaaacg cgtcgagcca
     3061 gtggttccgc agtccaccga gcagggctgg tatatcgaca aatcacgctt tgatttccat
     3121 atcgatccgg tgctgaacca gtcggtcggt ggcccggaaa acttttacat gtatcagctg
     3181 ggcgtgatgg gaacggcaga tttgtggctg acggaccatc tgctgaccat cggcagcctg
     3241 tttgcaaatc ttgccaacaa ctacgacaag tttaactaca ccaatccgcc acaggagtcg
     3301 cacttaccgc gcgttcgtac ccatgtgcgc gagtatgtgc agaacgatgc ctatgtgaat
     3361 aacctgcaag ccaactactt ccagcatctg ggcaacggct tctacggtca ggtctatgga
     3421 ggttatctcg aaaccatgtt tggcggtgcg ggggctgaag tgttgtatcg tccgctggac
     3481 agcaactggg cgtttggtct ggatgccaac tacgttaaac agcgcgactg gcgtagtgca
     3541 aaagatatga tgaaattcac cgactacagc gtgaaaaccg gacatctgac cgcctactgg
     3601 acgccatctt tcgcccagga cgtgttagtg aaagccagcg tcgggcagta tctggcaggg
     3661 gataaaggcg gcacgctgga gatcgccaaa cgctttgata gcggcgtggt ggtgggtggc
     3721 tatgccacga tcacgaatgt ttcaaaagag gagtacggcg aaggggactt caccaaaggc
     3781 gtgtatgtct cggtaccgtt ggatctcttc tcgtctggcc cgacacgcag ccgtgcggcg
     3841 attggctgga cgccgctgac gcgtgacggt ggtcagcaac ttgggcgtaa gttccagttg
     3901 tatgatatga ccagcgaccg tagcgtcaat ttccgctaag tcatgggaaa ggtgccagtt
     3961 ttcgcaatca ctggcaccct cattcttaat ggcctgttga gccatcagga acgcagttca
     4021 cccccggttt gcgatctggt gtgtaaaggc gaaaacattc tgtcttcctg tccgtcgata
     4081 tcggttgcag gtctgattgc ctgagcgact gggccatcat tgggctggag acattgcttt
     4141 gcggtaggtc ggctttatca gcagtaccga gcggaccagc ataagcagga agaacagaga
     4201 ctgataacat caaagcagca aaataaggct tcatttttac cacctttatc aggttacgtt
     4261 tcatttgttc cagaggaaca ttgtcgattt ttcgcgcatt gctggtggct gggaatcacc
     4321 tgaatgggtg atttttgaat taccggcttg ggtgcggttt gtcttgccgg atgcgccgcc
     4381 aggcgcggct tatccggcct acgggtaggt atatccggct ttggtggagg cgcgctccaa
     4441 atccaggttg aacaaaacat acacaaaaaa tatagatctc cgtcacattt ttgcgttata
     4501 caggaagctc gccactgtga aggaggtact gctatgacgt cactctctcg tccgcgcgtg
     4561 gagtttatct ccaccatttt gcagaccgta ctcaatcttg gcctgctgtg cctcggcctg
     4621 attttggttg tcttcctcgg caaagaaacg gtgcatctgg cagatgtgct gttcgcgcca
     4681 gaacaaacca gcaaatatga gctggtagaa ggactggtgg tttactttct ctatttcgaa
     4741 tttatcgcgc tgattgtgaa gtactttcag tccggttttc acttcccgct acgttacttt
     4801 gtctatattg ggatcaccgc cattgtgcgc ttgatcatcg tcgatcataa atcgccactg
     4861 gatgtgttga tctactcggc tgcgatcctg ttactggtga tcactctgtg gctgtgtaat
     4921 tcgaaacggc tgaagcggga gtaaaaagaa agcacgccga actggcgcgg cgtgctggac
     4981 aggaagatta cagcgtagca gtttgttgtg ttttcttcgt ttccggttcc cagagggctt
     5041 ccagctcctc aagggtttta cctttggttt ccgggacaaa tttccacata aatagtgctg
     5101 ccagaacgcc catacacccg taaatccagt aggagaaacc gttgtggaaa tgggccacca
     5161 gccaggagtt tttgtccatc atcgggaagg tccaggagac gaagtagttc gccagccact
     5221 gggccgccac cgcgattgcc agcgctttac cacgaatagc attcgggaag atttccgaca
     5281 gcggtaccca gcataccgga ccccaggaca tggcaaaggc agcaacatag aacagcatcg
     5341 acagtagcgc cacaataccc ggtgcctgag tgtaaaacgc ggtaccgagg ctaaacatac
     5401 cgattgccat tccgagtgcg ccgataattt gcagtggctt acgaccaaat ttatccaccg
     5461 tcataattgc cagaacggtg aaggtgaggt tgataactcc gacaataatg gtctgcaaca
     5521 gcgcgatatc cgtgctggcc cccagcgttt tgaacacttc cggcgcgtag tacagcacca
     5581 cattgatgcc gacaaattgc tggaagatgg agagcataac gccgatgaca atcacgccca
     5641 cgccaaacat cagcagacga ccaccggttt tgcggccatg atccagggag tgtttaattt
     5701 cctgtactgc ctgagttgca agcgtgttgc ccataatttt gcgcaggata ctttccgcct
     5761 gttcttgctt gccgcgtgac atcagccagc gaggactttc tggcacggta tacagcagca
     5821 ttaagaacag cagtgcaggg atacattccg aggcaaacat ataacgccag ccgtcagtat
     5881 tcagccagct ggcatcaccg gaacgggcaa taaaatagtt tacgcagtaa actaaaagtt
     5941 gcccgaaaat aatcgcaaac tggttaaatg aaaccagttt tccgcgaata tgagctggag
     6001 ccagttccgc aatatacatt ggtgaaagca ttgaggctaa accaacgcca ataccgccaa
     6061 taatacgata aataacaaat tccgggacat aacctgccag ataaataggt actgtgttgt
     6121 ccgggtttat agaggtaaaa ccaagttctg gccaggcaga acctacacca gaaataaaaa
     6181 acaggacagc agcaatctta agtgaatcac gacggccgaa gcggttactg caataaccac
     6241 cgagggcacc gccgatgatg caaccaatca gagcgctggc tacgcaaaat cctaacaggg
     6301 agttggcagc ggattcactt aagttttgtg gagcaacaaa gacggtattg agtgactcaa
     6361 cagtaccgga aataacggcg gtgtcgtagc caaataataa accacctaat gtagcgacta
     6421 aggtaatcga aaatatataa ctggaattat actgggtatt cattcagacc tgc
//
LOCUS       DAZOYL010000219         6465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZOYL010000219 DAZOYL010000000
VERSION     DAZOYL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.220"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..273)
                     /locus_tag="ACHUOY_003144"
     CDS             complement(<1..273)
                     /locus_tag="ACHUOY_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HFR0683858.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYL"
     gene            703..983
                     /locus_tag="ACHUOY_003145"
                     /pseudo
     CDS             703..983
                     /locus_tag="ACHUOY_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(1082..1618)
                     /gene="ssb1"
                     /locus_tag="ACHUOY_003146"
     CDS             complement(1082..1618)
                     /gene="ssb1"
                     /locus_tag="ACHUOY_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR0683859.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            1872..4694
                     /gene="uvrA"
                     /locus_tag="ACHUOY_003147"
     CDS             1872..4694
                     /gene="uvrA"
                     /locus_tag="ACHUOY_003147"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR0683860.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(4729..5085)
                     /locus_tag="ACHUOY_003148"
     CDS             complement(4729..5085)
                     /locus_tag="ACHUOY_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0683861.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(5089..5505)
                     /locus_tag="ACHUOY_003149"
     CDS             complement(5089..5505)
                     /locus_tag="ACHUOY_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR0683862.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(5616..6329)
                     /gene="aphA"
                     /locus_tag="ACHUOY_003150"
     CDS             complement(5616..6329)
                     /gene="aphA"
                     /locus_tag="ACHUOY_003150"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR0683863.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1554 a         1673 c         1672 g         1566 t
ORIGIN      
        1 aagatagcca cgaataatat tcaacattcg cttttgatgg gctggagtgc aaacttgccc
       61 ctgatagcgt tcagctgcat cgctcactaa atctgcctgg cgaatgacca gctcggattt
      121 atccagtgcc agttgagcaa aggttcggag ctgattattc acttctgatt ttgcccaaag
      181 gaaggcaatc catagcgaaa gaatgatggg aaagagaact aaaaagataa tgcccggcaa
      241 cgccagtaat tggtgtcgtg cacgatgact cattgctatc aatttcctgc ctgtttatac
      301 ccggccattg caaggtgaaa accctgggta ccgacgtctt tagataaatc ttacggtata
      361 aatagcactt ttgcctgatt tgaaattgat acctttctaa tcgtcacctg gatgaaaata
      421 attcattgat attcaaaatt aatttcctac aggaatgcag aggcggcggg aagatatgcc
      481 cttaatgcca ctgaaaaacc acataatctg tacagcaata aaagtcacgg cctaatatgg
      541 ctaacaaaca atcaattgtg atatagctca caaaattaat gaaacaaaca gagtgtttca
      601 tttttgtgtt ctgcaagccc cgcgatagcc agagtgtacc acgcctcccg tgaacaacgc
      661 cgcgctgtcc agggcatcgg ctcttttcag aggaattgtt tgatggctac cctcaccact
      721 ggcgtggttc ttcttcgctg gcagcttctt agtgccgtaa tgatgtttct ggccagcaca
      781 tcaacatccg ttttcgtcgg tctgattatg tcgggcttgc ggtgatcagc agcggtctgg
      841 gcgttgtttc cgcctgttgg ttcgcaatgg ggttgcttgg catcacaatg gcggacatca
      901 ccgctatctg gcacaacatc gagtcggtga tgatagaaga gatgaatcag acaccgccac
      961 aatggccaat gattttgacc tgatacgtag aagcctccat aacggaggct tcttttttat
     1021 ggctggcaga tgctttaatc atccacctta aaacaatata acctattgtt ttaatgacaa
     1081 atcagaacgg aatgtcatca tcaaagtcca tcggcggctc gttagacggc gctgccggag
     1141 cggactgctg cgggcgagac tgcgcgccgc cgctgaactg attgccaccc tgcggctgct
     1201 gaggctgccc ccaaccgccc tgcggctgac caccaccgat attaccacct gccggagcgc
     1261 caccaccctg acgaccaccc agcatctgca tggtgccgcc aacgttcacc acgacttctg
     1321 tggtgtagcg atcctgaccg gattgatcgg tccatttacg ggtacgcagc tgaccttcga
     1381 tataaacctg agaaccttta cgcagatatt cgctggccac ttctgccagt ttgccgaaca
     1441 gcacaacgcg gtgccattca gtctgttctt tcatctcgcc ggtcgcttta tcacgccagg
     1501 attcggaagt agccagcgta atgttggcaa ctgcgccacc atttggcatg tagcgtactt
     1561 ccgggtcctg acccagatta ccaacgagaa taaccttgtt tacgcctctg ctggccatgt
     1621 tcgtgtctcc tgaaaaaaat cgttctgaat aagtgtaaac gcgcgattgt accattacca
     1681 atagcgcttt tactatgttg tgacctcggt tccgggaaac aaacctggcc agacattgtt
     1741 acacaacact ccggataatg cattccaata ctgtatattc attcaggtca atttgtgtca
     1801 taattaaccg tttgtgatcg ccggtagcac catgccaccg ggcaaaaaag cgtttaatcc
     1861 gggaaaggtg aatggataag atcgaagttc ggggcgcccg cacccataat ctcaaaaaca
     1921 tcaacctcgt tatcccccgc gacaagctca ttgtcgtgac cgggctttcg ggttctggca
     1981 aatcctcgct cgctttcgac accttatatg ccgaagggca gcgccgttac gttgaatccc
     2041 tttccgccta cgcgcggcag tttctgtcac tgatggaaaa gccggacgtc gatcatattg
     2101 aggggctttc tcctgccatc tcaattgagc agaaatcgac gtctcataac ccgcgttcta
     2161 cggtggggac aatcaccgaa atccacgact atttgcgttt gttgttcgcc cgcgtcggcg
     2221 aaccgcgctg tccggaccac gacgtcccgc tggcggcgca aaccgtcagc cagatggtgg
     2281 ataacgtgct gtcgcagccg gaaggcaagc gtctgatgct gctcgcgcca atcattaaag
     2341 agcgcaaagg cgaacacacc aaaacgctgg agaacctggc aagccagggt tacatccgtg
     2401 ctcgtattga tggcgaagtc tgcgatcttt ccgatccgcc gaaactggaa ctgcaaaaga
     2461 aacacactat tgaagtggtg gttgatcgct tcaaggtgcg tgacgatctt acccaacgtc
     2521 ttgcggagtc gtttgaaacc gcgctggagc tttccggtgg tacagcggta gtggcggata
     2581 tggacgaccc gaaagcggaa gagctgctgt tctccgccaa cttcgcctgc ccaatttgcg
     2641 gctacagtat gcgtgaactg gagccgcgac tgttttcgtt taacaacccg gcaggtgcct
     2701 gcccgacctg tgacggcctt ggcgtacagc aatatttcga tcctgaccgc gtgatccaaa
     2761 accccgagct gtcactggct ggcggtgcga tccgtggctg ggatcgccgc aacttctatt
     2821 atttccagat gctgaaatcg ctggcagatc actataagtt cgacgtcgaa gcgccgtggg
     2881 gcagcctgag cgcgaacgtg cataaagtgg tgttgtacgg ttctggcaaa gaaaacattg
     2941 aattcaaata catgaacgat cgtggcgata cctccattcg tcgtcatccg ttcgaaggcg
     3001 tgctgcacaa tatggagcgc cgttataaag agacggaatc cagtgcggta cgtgaagaat
     3061 tagccaagtt tatcagcaat cgcccatgcg ccagctgcga aggaacgcgt ctgcgtcggg
     3121 aagcgcgcca cgtgtatgtc gagaatacgc cgctgcctgc tatctccgac atgagcatcg
     3181 gtcatgcgat ggaattcttc aacaatctca aactcgcagg tcagcgggcg aagattgcag
     3241 aaaaaatcct taaagagatc ggcgatcgtc tgaaattcct cgttaacgtc ggcctgaatt
     3301 acctgacact ttcccgctcg gcagaaacac tttccggcgg tgaagcccag cgtatccgtc
     3361 tggcgagcca gattggtgcg ggcctggttg gcgttatgta tgtactggac gagccgtcta
     3421 tcggcctgca ccagcgcgat aacgagcgcc tgttgggtac gcttatccat ctgcgcgatc
     3481 tcggtaatac cgtgattgtg gtggagcatg acgaagacgc aattcgcgcc gctgaccatg
     3541 tgatcgatat cggtcctggt gcgggtgttc acggcggtga agtggtcgca gaaggtccgc
     3601 tggaagcgat tatggcggtg cctgaatcgt tgaccgggca gtacatgagc ggtaaacgca
     3661 agattgaagt gccgaagaaa cgcgttccgg cgaatccaga aaaagtgctg aagctgacag
     3721 gtgcacgcgg caacaacctg aaggacgtga cgctcacgct gccagtcggt ctgtttacct
     3781 gcatcacagg ggtttcaggt tccggtaaat cgacgctgat taacgacaca ctgttcccga
     3841 ttgcccaacg ccagttgaat ggtgcgacca tcgccgaacc ggcaccgtat cgcgatattc
     3901 aggggctgga gcatttcgat aaagtgatcg atatcgacca aagcccaatt ggtcgtactc
     3961 cacgttctaa cccggcgacc tataccggcg tgtttacacc tgtgcgcgaa ctgtttgcgg
     4021 gcgtaccgga atcccgtgcg cgtggttata cgccaggacg tttcagcttt aacgtccgtg
     4081 gcgggcgctg cgaagcctgt cagggcgacg gtgtgatcaa agtggagatg cacttcctgc
     4141 cggatatcta cgtgccgtgc gatcagtgca aaggtaaacg ctataaccgt gaaacgctgg
     4201 aaattaagta caaaggcaaa accatccacg aagtgctgga tatgaccatc gaagaggcgc
     4261 gtgagttctt tgatgcggtg ccagctctgg cgcgtaagct gcaaacgttg atggacgttg
     4321 gtctgacgta cattcgcctg gggcagtccg caaccacact ttctggtggt gaagcccagc
     4381 gcgtgaagct ggcgcgtgag ctgtcaaaac gcggcaccgg gcagacgctg tatattctcg
     4441 acgagccgac caccggtctg cacttcgccg atattcagca actcctcgac gtgctgcata
     4501 aactgcgcga tcagggcaat accattgtgg tgattgagca caatctcgac gtgatcaaaa
     4561 ccgctgactg gattgtcgac ctgggaccag aaggcggcag tggcggcggc gaaatcctcg
     4621 tctccggtac gccagaaacc gtcgcggagt gcgaagcttc gcatacggca cgcttcctca
     4681 agccgatgct gtaatcgtta aggccgcttt ctgagcggcc ttttcctttc agagttgtac
     4741 cagcaattta cgcttttctt ccggtagtaa attcactgcc tgctgataag acgcatccac
     4801 cagataatag atttgcgaat ccggcagcga accgtcgaga tacacggtgc tccagtgcgc
     4861 tttattcaga tggcggctag gacgcacatc gctgtgctgc tgacgtagca gctccgccag
     4921 ctccgggctg gttttcagcg aaacagctgg gcgattttct acttctttca ccatcgcaaa
     4981 cagtacatct tccactttga tctgcgtcgc tttccagtcg ttatgcacgc tctgttctgc
     5041 gcctggtttt gccatgcaat attgtagcaa ctccgaaatg gtcatttttt actccccttg
     5101 tagtgtcgcg atgatgcgac gcgatccgcc gtggatgcga tgttccccca gccagatgcc
     5161 ttgccaggtg ccggtctgaa tacgcccttt atgtaacggc aatacaagcg atgttcccag
     5221 cattgaggat ttgatatgag aaggcatatc gtctgctccc tcatagtcat gctcataatt
     5281 tccgttgtcg ggaacggtgc ggaggaaaaa acgctccatg tcgtggcgta cggtgggatc
     5341 gcagttctca ttaagtgtca gagaggcgga ggtatgttgc agcaacagat gcagtaagcc
     5401 gatgttaacg cgcggcatat cagccagctg attcagaatt tcatccgtta ccagatgaaa
     5461 cccacgagat ttggcgctaa gcgtgagcgt cttttgatac cacatgtgct gctccttgat
     5521 aaaactctct taatcagttt gcagcaagac agcgaaagga taaaggtgtg attaaaaaaa
     5581 cagcattcgg gagagcatca cgctctcccg ctctgtcagt attctgaatt gacgatcacc
     5641 tcttcaccaa acgcacccgc ttgtggcaag ggtttgtagg tagagttgga ggcgcgcaga
     5701 atgcggatac cacgagcgcc gacatcgcgt gcggcggtaa tatcgttatc ggaatcgcca
     5761 taaaaaattc ggatattttt atcctgcagc cattgcgatt ttgtattttg ccctggttta
     5821 tcgcccgcaa agattaccgg attcatgttg gtggcaggaa tatgaaaatt atccgccagc
     5881 gtttttgaaa ccgtttctgt tttcgtcggg ctacgaccag tcacaaagaa gatcgcgtca
     5941 ccgcggcgta catgcatatc aatcagctgg cgagcgacct cttttggaat gctgaattca
     6001 tcccagccat tattcatttt ttcccagaac acaggatttt tcagataatc ttcgctttct
     6061 ggcgagaagg tttttttgcc gcgccagaac cccggactgg aaaaaagtac cgtgtcatcg
     6121 atatcaaacc ccaccgccat tggcggacgc cctgcgaggc tattttcaat ttgtgcgacc
     6181 gaaacccaat gaattggtgc ctgttcagca agcctggcaa cgttagtccc agggttaagc
     6241 ggtgaaggag atgaggccag ggcaacagcg gaactgttta gagcgaacaa taagcaaacg
     6301 gcactgattg cctgtgtgat cttgcgcata tttttcccta aatagtcagt atgttgaaac
     6361 tttttattgt gagatttgtt gcaaaaacca tctgaccata acgccagctg ccggggaaag
     6421 gaaggattat ctgcggtttt tgtgatgaaa agcaggatga ttata
//
LOCUS       DAZOYL010000220         6431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZOYL010000220 DAZOYL010000000
VERSION     DAZOYL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.221"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            145..789
                     /gene="adk"
                     /locus_tag="ACHUOY_003151"
     CDS             145..789
                     /gene="adk"
                     /locus_tag="ACHUOY_003151"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR0683864.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            921..1883
                     /gene="hemH"
                     /locus_tag="ACHUOY_003152"
     CDS             921..1883
                     /gene="hemH"
                     /locus_tag="ACHUOY_003152"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR0683865.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(1880..2839)
                     /gene="aes"
                     /locus_tag="ACHUOY_003153"
     CDS             complement(1880..2839)
                     /gene="aes"
                     /locus_tag="ACHUOY_003153"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR0683866.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            2991..4295
                     /gene="gsk"
                     /locus_tag="ACHUOY_003154"
     CDS             2991..4295
                     /gene="gsk"
                     /locus_tag="ACHUOY_003154"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR0683867.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(4428..6104)
                     /gene="ybaL"
                     /locus_tag="ACHUOY_003155"
     CDS             complement(4428..6104)
                     /gene="ybaL"
                     /locus_tag="ACHUOY_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR0683868.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(6342..>6431)
                     /locus_tag="ACHUOY_003156"
     CDS             complement(6342..>6431)
                     /locus_tag="ACHUOY_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683869.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         1637 a         1718 c         1632 g         1444 t
ORIGIN      
        1 ttcttttcat catctgcact ttccgcaaat tatctcgcca ttaaccgttt cagcctcagg
       61 tgcctttctt gaggcaatcg cctgttggtg gtatcgttta tcgctttttc aaaaaaatcg
      121 acacatttta aggggatttt cgcaatgcgt atcattctgc ttggcgctcc gggcgcgggg
      181 aaagggactc aggctcagtt catcatggag aaatatggta ttccgcaaat ctccactggc
      241 gatatgctgc gtgctgcggt caaatctggc tccgagctgg gtaaacaagc aaaagacatt
      301 atggatgctg gcaaactggt caccgacgaa ctggtgatcg cgctggttaa agagcgcatt
      361 gctcaggaag actgccgtaa tggtttcctg ttggacggct tcccgcgtac cattccgcag
      421 gcagacgcga tgaaagaagc gggcatcaat gttgattacg ttctggaatt cgacgtaccg
      481 gacgaactga ttgttgatcg tatcgtaggc cgccgcgttc atgcgccgtc tggtcgtgtt
      541 tatcacgtta aattcaatcc gccgaaagta gaaggcaaag acgacgttac cggtgaagaa
      601 ctgactaccc gtaaagacga tcaggaagaa accgtgcgta aacgtctggt tgaataccat
      661 cagatgactg caccgctgat cggctactac ttcaaagaag cggaagcggg taacaccaaa
      721 tacgcgaaag ttgacggcac caagtcggtt gctgaagttc gcgctgatct ggaaaaaatc
      781 ctcggctaat caagccgcag cagatctgct ttcgggcagg tctgctcatc cacctcaggc
      841 aatacctctc acagcaatta gttcttcttc ctcacttttc cgctacaatt atcaacaagt
      901 tgaatcgata agaggcggta atgcgtcaga ctaaaatcgg tatcctgctg gcaaacctgg
      961 gtacgcccga tgcccccaca cctgaagcgg taaaacgcta tctgaaacaa tttttaagcg
     1021 acagacgcgt ggttgatacc tcacggttgt tatggtggcc attgctgcgc ggcgttattt
     1081 tgccgctgcg ctcgccgcgt gtggcgaagc tgtacgcctc ggtctggatg gaaggtggct
     1141 cgccgctgat ggtttacagc cgccagcaac agcaggcgct ggcacaacgt ttaccggaga
     1201 cgcccgtagc gctgggaatg agctacggct cgccatcact ggaaagcgcc gtcgatgaac
     1261 tcctggcaga gcatgtagat catattgtgg tgctgccgct ttatccgcaa ttctcctgtt
     1321 ctacggtcgg tgcggtatgg gatgaactgg cacgcattct ggcgcgcaaa cgtagcattc
     1381 cggggatatc gtttattcgt gattacgccg ataaccacga ttacattaat gcactggcga
     1441 acagcgtacg cgcttctttt gccaaacatg gcgaaccgga tctgctgctg ctctcttatc
     1501 atggcattcc ccagcgttat gcagatgaag gcgatgatta cccgcaacgt tgccgcacaa
     1561 cgactcgcga actggcttcc gcactgggga tggcaccgga aaaagtgatg atgacctttc
     1621 agtcgcgctt tggccgggaa ccctggctga tgccttatac cgacgaaacg ctgaaaatgc
     1681 tcggagaaaa aggcgtaggt catatacagg tgatgtgccc gggctttgct gcggattgtc
     1741 tggagacgct ggaagagatt gccgagcaaa accgtgaggt cttcctcggt gcgggcggga
     1801 aaaaatatga atatattcca gcgcttaatg ccacgccgga acatatcgaa atgatggcta
     1861 atcttgttgc cgcgtatcgc taaagctgag cggtaaagaa ctgagcgccg tcgcgaagag
     1921 cctcgtcggc ggttttcatc atccgtgaat aatgcaaaaa ggcatgcagc gtacctgggt
     1981 agagtttgaa ctcacagggc tgctgatgcg ccgctaacgt ctggtaaagc agacggctgt
     2041 catccagcag cggatcgaac tccgccccgg caataaaaca gggcggaact tcgcgagtga
     2101 gatcattatt aaacagacag taatacggtg actcgcggtc cgcgtcgttg cttaaatacg
     2161 cctcttcata catctgcaaa tcctgttgcg ttaagccatc ccagacaccg cccaacagac
     2221 gacgagtcac ggaatcccgt aatccgtaaa gcccatacca cagcaaaaca cccgcaattt
     2281 taccgcaatc gatctgttta tcacgcaacc acaacgcact ggcgagcgcc agcatggcac
     2341 ctgcggaatc accggcaaag ccaatgcggg acatattgat ttgataatcc tccgcctgct
     2401 ggtggaaata acaacaagca gccacaattt cctctatcgc ttgcggaaaa cgcgcttcag
     2461 gtgaaagggt gtaatcaata ccaatcaccg tacattggct gtagcttgcc agcaggcgca
     2521 tgatgcgatc gtgggtatcg agattgccga gaataaaacc gcctccatgc aaataaaata
     2581 gcgtcgccgg gctatctggc tgcggacaaa agagacgtgt ttccacctgc ccatattttg
     2641 ttggaaccat gtaagctctg gttgccattt ctggagcgcc cgcattccag aatcggcgct
     2701 caagcgtgta atactgtcgt tgctcagcaa tcgttcccgt tgcgggccag gacggtaaat
     2761 ccggctgaag agtattcaca acggtcttca tttcagcaga aataaggtcc agaacaggta
     2821 gtttgttttc cggcttcata caaaaactcc tttcaaatta cgtcattgta aggaaccact
     2881 gccatgaaaa tgcgatcccg cctgctgata ttgaaactgg ctgcgtctcg cgcgctcccg
     2941 tcagattgtg ttaacattcg ccgctcagtt aaccacccgt aaaaacaacc atgaaatttc
     3001 ccggtaaacg taaatccaaa cattacttcc ctgtaaatgc acgcgatccg ctgcttcagc
     3061 aattccagcc agaaaacgaa accagcgccg cctgggtcgt aggtattgac cagacgctgg
     3121 tcgatattga agcgaaagtg gatgatgaat tcattgagcg ttatggatta agcgccgggc
     3181 attcactggt gattgaggat gacgtagccg aagcgcttta tcaggagcta aaacagaaaa
     3241 acctgattac ccatcagttt gcgggtggca ccattggtaa caccatgcac aactactcgg
     3301 tgctcgcgga cgaccgttcg gtgctgctgg gcgtcatgtg cagcaatatt gaaattggca
     3361 gttatgccta tcgttacctg tgtaacactt ccagccgtac cgatcttaac tatctacaag
     3421 gcgtggatgg cccgattggt cgttgcttta cgctgattgg cgagtccggg gaacgtacct
     3481 ttgctatcag tccaggccac atgaaccagc tgcgggctga aagcattccg gaagatgtga
     3541 ttgccggagc ctcggcactg gttctcacct cttatctggt gcgttgcaaa ccgggtgaac
     3601 ccatgccgga agcaaccatg aaagccattg agtacgcgaa gaaatataac gtaccggtgg
     3661 tgctgacgct gggcaccaag tttgtcattg ccgagaatcc gcagtggtgg cagcaattcc
     3721 tcaaagatca cgtctctatc cttgcgatga acgaagatga agccgaagcg ttgaccggag
     3781 aaagcgatcc gttgttggca tctgacaagg cgctggactg ggtagatctg gtgctgtgca
     3841 ccgccgggcc aatcggcttg tatatggcgg gctttaccga agacgaagcg aaacgtaaaa
     3901 cccagcatcc gctgctgccg ggcgctatag cggaattcaa ccagtatgag tttagccgcg
     3961 ccatgcgcca caaggattgc cagaatccgc tgcgtgtcta ttcgcacatt gcgccgtaca
     4021 tgggcgggcc ggaaaaaatc atgaacacta atggagcggg ggatggcgca ttggcagcgt
     4081 tgctgcatga cattaccgcc aacagctacc atcgtagcaa cgtaccaaac tccagcaaac
     4141 ataaattcac ctggttaact tattcatcgt tagcgcaggt gtgtaaatat gctaaccgtg
     4201 tgagctatca ggtactgaac cagcattcac ctcgtttaac gcgcggcttg ccggagcgtg
     4261 aagacagcct ggaagagtct tactgggatc gttaagttat cgtcggttcg taggccagat
     4321 aaggcgttca cgccgcatct ggcatttggc tctcgatgcc tgatgcgacg ctggcgcgtc
     4381 ttatcatgcc tacatatttt tcatatttta catccggcaa ccaccgttta ccccgtcacc
     4441 acttcacccg ccggtggcgt ttccagcagt tccagcatag tacgggcaat ttcacgttca
     4501 cccatcacta cctgattcgc gccacgttcg gtgatatacg tcacttcatc gtcataatgg
     4561 gcgcgggcga taatctcaat atccgggttt ttcgcgcggg cagacgccac aatctcaccc
     4621 gcttcataac cgttggggat cgtcaggatc agccattttg cacattccag atgcgccagt
     4681 tgcataattt cttcgttcgc tgcattgccc aatacagcgc ggaccccacg ctctcgcagc
     4741 tcatcaacac gggttcgtga cgtctcaatc accaccagcg gaatatcaga ggcgagcaat
     4801 ttctccccca gcaggctgcc tacacgaccg taacccacca atagcgcatg gttgcaaata
     4861 tccactggga tctgtttctc ttcttcgatt gcctcttcca gcgtctgctc ttccagcgtt
     4921 tcggtcttcg ccagatattt ctccagtagt gcgaacagta ccgggttgag cataatcgac
     4981 aggatcgccc ctgccagtac caggttttgt ccggcctgcg gcagtaaatt caatgccatt
     5041 cccagtcccg ccaggataaa cgcgaactca ccaatctgcg ccaggctggc ggcgatggtt
     5101 aatgccgtac gttgggagtg accaaacaga cggacaagga acagggccgc gaccgattta
     5161 ccaaacagaa taattgccag cgtcgccagc actgccagcg gttgctgaat cagaattaac
     5221 ggatcaaaca acatcccgac ggagacaaaa aacagcaccg caaacgcgtc gcgcaatggc
     5281 agcgtatcgt gggcggcacg gtgactcagt tcagactcgt tcagtaccat cccggcaaag
     5341 aacgcgccaa gtgcgaagga gacgtcaaac aactccaccg caccaaaggc aatccctaac
     5401 gccagcgcca gcaccgacag ggtaaatagc tcgcgagaac cggttgccgc gctgcgtgcc
     5461 ataatccacg gcaccagacg gcgacctacc agcatcataa tggcgataaa tgcgatcact
     5521 ttgccgatgg tgatccccat atcgactgca agagtggcaa agcccacatc gccctgttcc
     5581 atcattcctg ccactgcggg cagcaacacc agcgtcagaa ccattaccag gtcttccaca
     5641 atcaaccaac cgatggcgat ttgcccacgc tgactgtcaa ttaattgccg ttcttcaagt
     5701 gcgcgcagta acaccacggt actggcggtg gaaagacata aaccgaacac gataccggtc
     5761 attaacgacc agcccagcac ggcagagagc gccataccca gcagcgtcgc cacggctatc
     5821 tgggcgatcg caccgggaat ggcgatggcc tttaccgcca tcaaatcctt cagcgaaaag
     5881 tgcaaaccga cgccaaacat caacagaatg acgcccagtt cagccagttc cggcgcaagc
     5941 ttggtatcgg caacaaagcc cggagtgaat ggtcctgcca gcacacccgc taacagatat
     6001 cccaccagag gagaaatacg tagtttattg gccagcatgc cgaggataaa ggcgagcaca
     6061 aggccgccaa caatggtggt gataagcggg gtggcgtgat gcattccgtc tccttttcct
     6121 ggtggttatt gtccattttt ggccgggaaa accaaaatta caggtaatag tttatgacaa
     6181 tttcattgat gatgttcatg aataattatt gaattttgca gaaaaatgga attagctgca
     6241 aaaaaagcac ggatcggaaa acagaaggcg gtttaacaga ggaaaggctt atggcgactg
     6301 taaaacgatg cagccaaagt ttgcctttgg ctgcaatgaa atcagtcttt atgccggtta
     6361 tcaggcagga atatggtcaa catccccaat agtggcagga aagcacagat tttatagact
     6421 aactcgatgc t
//
LOCUS       DAZOYL010000221         6413 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZOYL010000221 DAZOYL010000000
VERSION     DAZOYL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.222"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1245
                     /locus_tag="ACHUOY_003157"
     CDS             <1..1245
                     /locus_tag="ACHUOY_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR0683870.1"
                     /translation="YLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDN
                     ADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTI
                     DGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASA
                     LEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSK
                     KIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEI
                     KALIEDSVARVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVAL
                     AVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRPA
                     SEQPPAQPRLRIAEQDPNWETF"
     gene            1291..2892
                     /gene="tap"
                     /locus_tag="ACHUOY_003158"
     CDS             1291..2892
                     /gene="tap"
                     /locus_tag="ACHUOY_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR0683871.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            2911..3771
                     /gene="cheR"
                     /locus_tag="ACHUOY_003159"
     CDS             2911..3771
                     /gene="cheR"
                     /locus_tag="ACHUOY_003159"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR0683872.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLMDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            3774..4823
                     /gene="cheB"
                     /locus_tag="ACHUOY_003160"
     CDS             3774..4823
                     /gene="cheB"
                     /locus_tag="ACHUOY_003160"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR0683873.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            4838..5227
                     /gene="cheY"
                     /locus_tag="ACHUOY_003161"
     CDS             4838..5227
                     /gene="cheY"
                     /locus_tag="ACHUOY_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR0683874.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            5238..5882
                     /gene="cheZ"
                     /locus_tag="ACHUOY_003162"
     CDS             5238..5882
                     /gene="cheZ"
                     /locus_tag="ACHUOY_003162"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR0683875.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            6084..>6413
                     /locus_tag="ACHUOY_003163"
     CDS             6084..>6413
                     /locus_tag="ACHUOY_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR0683876.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
BASE COUNT         1541 a         1580 c         1856 g         1436 t
ORIGIN      
        1 tatctagatt atggcaatac tggagcttat ttcgctcagc caacccaggg aatgcaaaat
       61 gcaatgggcg aagcgtttgc tcagtacgcc ctcagcagtg aaaaactgta tcgcgatatc
      121 gtcactgaca acgcagatga ttaccgattt gcccagtggc aactggcggt tatcgcgctg
      181 gtggtggtat tgattctgct ggtggcgtgg tacggcattc gccgtatgtt gcttactccg
      241 ctggcaaaaa ttattgctca cattcgcgaa atcgccggtg gtaacctagc gaataccctg
      301 accattgacg ggcgcagtga aatgggcgac ctggcgcaga gcgtttcaca tatgcaacgc
      361 tctttgactg acaccgtcac tcatgtccgc gaaggttcag atgccatcta tgccggtacc
      421 cgtgaaattg cggcgggcaa caccgatctt tcctcccgta ctgaacagca ggcatccgcg
      481 ctggaagaaa ctgccgccag catggagcag ctcaccgcga cagtgaagca aaacgccgat
      541 aacgcccgcc aggcctcgca actggcgcaa agtgcctccg acaccgccca gcacggcggc
      601 aaagtggtgg atggcgtagt gaaaacgatg catgagatcg ccgatagttc gaagaaaatt
      661 gccgacatta tcagcgttat cgacggtatt gccttccaga ctaatatcct cgcgctgaat
      721 gccgcggttg aagccgcgcg tgcgggtgaa cagggccgtg gttttgccgt ggtggcgggt
      781 gaagtgcgta atcttgccag tcgcagcgcc caggcggcaa aagagatcaa agccctcatt
      841 gaagactccg tcgcacgcgt tgataccggt tcggtgctgg tcgaaagcgc cggggaaaca
      901 atgaacaata tcgtcaatgc tgtcactcgc gtgactgaca ttatgggcga gattgcatcg
      961 gcatcggatg aacagagccg tggcatcgat caagtcgcat tggcggtttc ggaaatggat
     1021 cgcgtcacgc aacagaacgc atcgctggtg caggaatcag ctgccgccgc cgctgcgctg
     1081 gaagaacagg cgagtcgttt aacgcaagcg gtttccgcgt tccgtctggc agccagccca
     1141 ctcaccaata aaccgcaaac accatcccgt cctgccagtg agcaaccacc ggcacagcca
     1201 cgactgcgaa ttgctgaaca agatccaaac tgggaaacat tttgacgagt atttactaac
     1261 gcggtcattg ccgcctgatg gggagcgttg atgtttaatc gtattcgaat ttcgaccacg
     1321 ctgtttttaa ttttgattct ctgcgggatc ttgcagattg gcagtaacgg catgtctttt
     1381 tgggcatttc gcgacgattt gcaacgactg aatcaggtcg agcagagcaa tcagcaacgt
     1441 gcggcattag cgcaaactcg ggcggtaatg ttacaggcca gtaccgcgct gaacaaagcg
     1501 ggcactctga cggcacttag ctatccggcg gatgacatta aaacgttgat gacgacggcg
     1561 cgcgccagtc tgacgcaatc caccacgctg tttaaaagtt ttatggcgat gactgcgggc
     1621 aacgagcacg tcagggcatt gcaaaaagag acggagaaaa gttttgcccg ctggcacaac
     1681 gatctcgaac atcaggcgac ctggcttgaa agtaatcaac tttcggattt cctcactgcg
     1741 ccggtgcagg gatcacagaa tgcgtttgac gttaactttg aggcctggca gctggagatc
     1801 aaccatgtgc tggaagccgc cagtgcgcaa agccagcgta actatcagat ttcgacactg
     1861 gtgtttatca gcatgattat tgttgcggcg atctacatca gcagtgcgct gtggtggacg
     1921 cgcaagatga ttgttcaacc actggccatt atcggtagcc attttgacag cattgctgcg
     1981 ggtaatctgg cgcgtccgat tgcggtatat ggtcgtaatg agatcaccgc catttttgcc
     2041 agtctgaaga ccatgcagca ggctttgcgt gggacggtaa gtgatgtgcg taagggaagc
     2101 caggagatgc acattggtat cgcggagatt gtcgcaggca ataacgatct ctcaagtcgt
     2161 accgaacagc aggcggcatc gctggcacaa acggccgcca gtatggagca attaaccgcc
     2221 acggtagggc aaaacgccga taacgcacga caggcgtcgg aactggcaaa aaatgccgcg
     2281 acaacggcgc aggcgggcgg tgttcaggtc agtaccatga ctcacaccat gcaggagatc
     2341 gccaccagct cgcaaaaaat tggcgacatt atcagcgtta tcgacggaat tgctttccag
     2401 accaatattc tggccctgaa tgcggcagtg gaagcggctc gcgccggaga gcaggggcgt
     2461 ggttttgcgg tagtggcagg tgaagtgcgc aatcttgcca gccgtagcgc gcaggcagca
     2521 aaagagatca aagggctgat cgaagagtca gtcaatcgtg tccagcaggg ttcgaaactg
     2581 gtgaataacg ccgccgcgac catgatcgat attgtcagtt cggtgacccg cgtgaacgac
     2641 attatgggag aaattgcctc tgcgtcggaa gaacaacagc gggggattga gcaggttgca
     2701 caggctgtca gccagatgga tcaggtgact cagcagaacg cctcgctggt agaagaagcg
     2761 gcggtggcaa cggaacaact ggcgaatcag gccgaccatc tttcgtcgcg tgtggcggta
     2821 tttacccttg aagaacatga agtagcacga catgagtcgg cgcagttaca aattgcgcca
     2881 gtggtatcct gaagtgattg agaaggcgct atgacttcat ctctgccctg tgggcaaacg
     2941 tctttattgt tacagatgac cgagcgcctg gcgctttccg acgcgcattt tcggcggata
     3001 agtcaattga tctatcaacg agccgggatc gttctggctg accataaacg cgacatggtt
     3061 tacaaccgac tggttcgtcg tttgcgttcg ctgggactga tggatttcgg tcattatctg
     3121 aacttgctgg aatctaatca gcacagcggt gagtggcagg cgtttatcaa ttcgctgacc
     3181 acgaatctga cggcattttt ccgtgaggca caccatttcc ctctgctcgc ggatcacgca
     3241 catcgccgtt ctggcgagta tcgcgtatgg agcgcggcgg cttcgaccgg cgaagagccg
     3301 tacagcattg cgatgacgct ggctgacaca ttgggcaccg cgcccggacg ctggaaagtg
     3361 tttgccagtg atatcgacac cgaagtgctg gaaaaagcca gaagcggtat ttatcgccat
     3421 gaagagttga aaaacctgac gccgcagcaa ctgcaacggt atttcatgcg agggacggga
     3481 ccgcatgaag ggctggtacg cgtgcgtcag gagctggcga actatgttga ttttgccccg
     3541 ctgaatctac tggcgaaaca gtacaccgtg ccggggccgt ttgatgcgat cttctgtcgt
     3601 aacgtcatga tctacttcga tcaaaatacc cagcaggaga ttttgcgccg ctttgttccg
     3661 ctccttaaac ccgacggatt gctgtttgcg ggttactctg aaaactttag ccaccttgag
     3721 cgccgcttca cgctgcgtgg tcagacggtg tatgcgctaa gtaaggatta acgatgagca
     3781 aaatcagggt gttatctgtc gatgattcgg cactgatgcg ccagatcatg acagaaatca
     3841 tcaacagcca tagcgacatg gaaatggtgg cgaccgcgcc tgatccgctg gtcgcgcgtg
     3901 acttgattaa gaaattcaat cccgatgtgc tgacgctgga tgttgaaatg ccgcggatgg
     3961 acggactgga tttcctcgaa aaattaatgc gtttgcgtcc aatgcccgtt gtgatggttt
     4021 cttccttgac cggcaaagga tcagaagtca cgctgcgcgc gctggagctg ggggcaatag
     4081 attttgtcac caaaccgcaa ctgggtattc gcgaaggaat gctggcgtat agcgaaatga
     4141 ttgctgaaaa ggtgcgtacg gcagcaaagg cgagccttgc agcacataag ccattgtcag
     4201 caccgacaac gctgaaggcg ggtccgttgt tgagttctga aaaactgatt gcgattggtg
     4261 cttcaacggg tggaactgag gcaattcgtc acgtactgca accgttgccg ctttccagcc
     4321 cggcattgtt aattacccaa catatgccgc ccggtttcac ccgctctttt gccgacagac
     4381 ttaataagct ttgccagatc ggggttaaag aagccgaaga cggagaacgt gtcttgccgg
     4441 ggcatgccta tattgcgccg ggcgatcggc atatggaact ggcgcgtagt ggcgcaaatt
     4501 atcaaatcaa aattcacgac ggcccggcgg ttaaccgtca tcggccttcg gtagatgtgt
     4561 tgttccattc tgtcgccaaa caggcggggc gtaatgcggt tggggtgatc ctgaccggta
     4621 tgggcaacga cggcgcggcg ggaatgttgg cgatgcgtca ggcgggggca tggacccttg
     4681 cgcaaaacga agcaagttgc gtggtgttcg gcatgccgcg cgaggccatc aatatgggtg
     4741 gtgtctgcga agtggtcgat cttagccagg taagccagca aatgttggca aaaattagtg
     4801 ccggacaggc gatacgtatt taaatcagga gtgtgaaatg gcggataaag aacttaaatt
     4861 tttggttgtg gatgactttt ccaccatgcg acgcatagtg cgtaacctgc tgaaagagct
     4921 gggattcaat aatgttgagg aagcggaaga tggcgtcgac gctctcaata agttgcaggc
     4981 aggcggttat ggatttgtta tctccgactg gaacatgccc aatatggatg gcctggaatt
     5041 gctgaaaaca attcgtgcgg atggcgcgat gtcggcattg ccagtgttaa tggtgactgc
     5101 agaagcgaag aaagagaaca tcattgctgc ggcgcaagcg ggggccagtg gctatgtggt
     5161 gaagccattt accgccgcga cgctggagga aaaactcaac aaaatctttg agaaactggg
     5221 catgtgagga tgcgactatg atgcaaccat caatcaaacc tgctgacgag cattcagctg
     5281 gcgatatcat tgcgcgcatc ggcagcctga cgcgtatgct gcgcgacagt ttgcgggaac
     5341 tggggctgga tcaggccatt gccgaagcgg cggaagccat ccccgatgcg cgcgatcgtt
     5401 tgtactatgt tgtgcagatg accgcccagg ctgcggagcg ggcgctgaac agtgttgagg
     5461 cgtcacaacc gcatcaggat caaatggaga aatcagcaaa agcgttaacc caacgttggg
     5521 atgactggtt tgccgatccg attgaccttg ccgatgcccg tgaactggta acagatacac
     5581 gacaatttct ggcagatgta cccgcgcata ccagctttac taacgcgcaa ctgctggaaa
     5641 tcatgatggc gcaggatttt caggatctca ccgggctggt cattaagcgg atgatggatg
     5701 tcattcagga gatcgaacgc cagttgctga tggtgctgtt ggaaaacatc ccggaacagg
     5761 agtcgcgtcc aaaacgtgaa aaccagagtt tgcttaatgg acctcaggtc gataccagca
     5821 aagtcggcgt ggtagccagt caggatcagg tggacgattt gttggatagt cttggatttt
     5881 gatttgtatt gcctgatgtg gcgtgaccac gtcatatcag gcggtctgat aaggcgatga
     5941 cgccgcatcc gacaaacgca cactatgctt gatgtgtggc agcaaaagcc ctaaatcccg
     6001 cctgttttgc cccttactca aaccattgaa cgctttgcgc tctggcatca tttaagctta
     6061 atactctttc caggattggc gacgtgtctg acgagagcga cgacaaaaca gaagccccca
     6121 cacctcaccg actagaaaaa gcgcgggaag aggggcaaat cccgcgttcc cgtgaactga
     6181 cctcgttgct gattttgtta gtgggcgtgt gtgttatctg gtttggcggt gtgtcgctgg
     6241 cccgtcgatt gtcgggcatg ctctccgctg ggctgcattt tgatcacagt atttatatga
     6301 acgcatcccg tttgcaaggc atccattgtt ttgacatgac gggtaggttg cagctttata
     6361 tccggcttta ttgcagccgt aacgctgcga gcccctatgt aattcgccga ctc
//
LOCUS       DAZOYL010000222         6372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZOYL010000222 DAZOYL010000000
VERSION     DAZOYL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.223"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUOY_003164"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUOY_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0683877.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHUOY_003165"
     CDS             2219..2767
                     /locus_tag="ACHUOY_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR0683878.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUOY_003166"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUOY_003166"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR0683879.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHUOY_003167"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHUOY_003167"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR0683880.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHUOY_003168"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHUOY_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR0683881.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1604 a         1564 c         1611 g         1593 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcagtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc at
//
LOCUS       DAZOYL010000223         6302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZOYL010000223 DAZOYL010000000
VERSION     DAZOYL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.224"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..355
                     /gene="mcbR"
                     /locus_tag="ACHUOY_003169"
     CDS             <1..355
                     /gene="mcbR"
                     /locus_tag="ACHUOY_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0683882.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(391..2493)
                     /gene="pqqU"
                     /locus_tag="ACHUOY_003170"
     CDS             complement(391..2493)
                     /gene="pqqU"
                     /locus_tag="ACHUOY_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR0683883.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2735..3796
                     /locus_tag="ACHUOY_003171"
     CDS             2735..3796
                     /locus_tag="ACHUOY_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR0683884.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="ACHUOY_003172"
     CDS             complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="ACHUOY_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR0683885.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5677..6294
                     /locus_tag="ACHUOY_003173"
     CDS             5677..6294
                     /locus_tag="ACHUOY_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR0683886.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1697 a         1592 c         1509 g         1504 t
ORIGIN      
        1 caccccgcaa gacctgacgg aactccagga attgctcgag aaattacagc aggcgcaaga
       61 aaagggcgac atggaacaaa tcattaatgt aaacaggcta tttcgcttag cgatttatca
      121 tcgctcaaat atgcccatcc tgtgtgagat gattgagcaa ctgtgggtca ggatggggcc
      181 tggtttacat tatctctatg aagcgattaa tccagcggaa ttacgggagc atatagaaaa
      241 ctatcatctg ttactcgctg cgttaaaagc aaaagacaaa gagggatgca gacattgtct
      301 tgctgaaatt atgcaacaaa atattgctat tttataccag caatacaatc gttaaagtaa
      361 atgcccggca aataccgggc atcaaggtaa ttactcaaat ctccacgcaa tattcatgcc
      421 gacaccataa tttcgtccgg gcgaaggttc gtaatatcgc ccgtttgact cattgacaat
      481 gacagaacca acgtattctt tatcgaataa attatcgaca cgaccaaata aatccacagt
      541 taaattgtgg taattgtatt tatacccggt gaataagccg acgagagtat aagacggcgc
      601 ttttgccgta ttttcatcat ctgccataat atcgcccata taacgcgctt ccgtgcctgc
      661 ataccaacca tcttccggta cataacctat cgacgcaaag cccatattac gggcgatccc
      721 tggcatccga ttaccgttac aatcctgttc attgcaaaca ttgctgcgat aggtcgcatc
      781 aagccaggtc caggacgcgt ttacgcgaaa atctcctgcg aaacgttgat cccatgccag
      841 ttcagcgcct tgacgacggg tctttccggc atttttgtaa gtcgtacgcc caccgctact
      901 gctatcgaca acaatttcat catcagtgtc ggtctgaaac aatgcgagac taagcagccc
      961 atcaccaata cgcgttttac tgccgatctc aattgtatcg ttggtggatg gttttaaacc
     1021 aaagttcata ccgctttgcc catcagcacg ataagacagc tcattaatcg tcggcgtttc
     1081 aaaacctcgc ccggctgcca gatagatatt ccaggcatcg gtcattgcat attttaacga
     1141 accggcaggt agccatttat gataactggc atcaccgctg tcatcgccgt tacccggagt
     1201 aacgtaatgg tcgttggaat caaaccacac ggagctgtag cgcacgccag catccagcga
     1261 cagtttttcg ctcagctgcc actgcgtctg taaataggga tcgatgttcc acatcagatt
     1321 gcgttcgtcg cgacgcaact cacctttttg cccgtactcc ggcatgccgc tattcaggcg
     1381 gaagttattg tagcccttgc ggttttcact catgttttcg tagttcaggc cggtagtgaa
     1441 cgtgaccgga acgcccagtt caccacggtg tgtccagcgg ctgtctatcc cctggtaatg
     1501 gcgttgcagg gtaatcacgc cgcccgcatg tgacgggtta agttgtggtg ccatgggtat
     1561 tgactggtac tgggtcgttt ctcgctctcc ggcatacatc atcacactca tatcatcttg
     1621 cgcgctcagg ctacgctcat agcgcaaccc agcctgagtt tgcttgatgg tttttcgcgt
     1681 gtcgtactgt tctgcacgag gcgcttgttg tggattagcc ttccattctg ctttggttag
     1741 cccacccggg tcatctgctt tgatatccac actattgaaa atcagactta atttgctggc
     1801 ttcatcaatg cgtacgccca gtttggcatt ggctaaattt ttctgtgcgc cactatggtc
     1861 acgatagccg tgggtcgtaa aacgcgtggt tgagacggtg taatcgacat cgccaggctg
     1921 tgtgccgtct cccgttgcgc ccgttgcttt cagcccatag cgccagctgc caaaactgcc
     1981 gtagtaacta ctggcttcaa tggttggtgg ctgttgtccg gtctgggtgg tgacattcat
     2041 taccccacca gacgcgttgc catacagggc agagaagggg ccacgcagca cttccacatt
     2101 ttgcacactg cttaaatcga tgttggatgt ttgcccttgc ccgtcgggca tggtggcggg
     2161 aataccgtcc acatacaggc gaataccgcg aataccgtaa gtggagcggg agccaaatcc
     2221 gcgaatcgac agctgtaaat cttgcgcata gttctgccgg ttttgtacct gcaaaccagg
     2281 cacgtcggtc agtgattcgg acaagttaat gcgcggtgtt gccaggcgca tctcctcgcc
     2341 atccaccacg cttactgctg ctggggtatc cagttctgaa accacctgcg gtgcggcact
     2401 gacaatcatg gtctgttcat cagcggcaaa aacaacgggg gaaagggcaa gtagtgcggg
     2461 caaaacggtc tgtcggacgg aaaaaatctt catgaaaaaa gccaggttaa gaatgggaaa
     2521 acgccgttat ggtaataaaa ttgtaaattc atggaaaacg aaacggcacg atacgttaag
     2581 taattgagaa aattgtagtc gtaacggcaa gaaatgctcc acatttgaga aaataatgat
     2641 taccattccc atttataaca agagcgtaac gatgattacg cttagcgaag cattgtgaag
     2701 cagcaaaaat atcggttcat caaagggagt cgtcatgcat ttacgtcatc tgttttcatc
     2761 gcgcctgcgt ggttcattac tgttaggttc attgcttgtt gcttcatcat tcagtacgca
     2821 ggccgcagaa gaaatgctgc gtaaagcggt aggtaaaggt gcctacgaaa tggcttatag
     2881 ccagcaagaa aacgcgctgt ggctcgccac ttcgcaaagc cgcaaactgg ataaaggcgg
     2941 cgtggtttat cgtcttgatc cggtcactct ggaagtgacg caggcgatcc ataacgatct
     3001 caagccgttt ggtgtcacca tcaataacac gactcagaca ctgtggtttg gtaacactgt
     3061 aaacagcgcg gtcacggcga tagatgccaa aacgggcgag gtgaaaggcc gtctggtgct
     3121 ggatgatcgt aagcgtacgg aagaggtgcg cccgctgcaa ccgcgtgagc tggtagctga
     3181 cgatgccacg aacaccgttt acatcagtgg tattggtaaa gagagcgtga tttgggtcgt
     3241 tgatggcgag aatatcaaac tgaaaaccgc catccagaac accggtaaaa tgagtaccgg
     3301 tctggcgctt gatagcaaag gcaaacgtct ttacaccact aacgctgacg gcgaattgat
     3361 taccatcgac accgccgaca ataaaatcct cagccgtaaa aagctgctgg atgacggcaa
     3421 agagcacttc tttatcaaca ttagccttga taccgctagg cagcgtgcat ttatcaccga
     3481 ttctaaagcc gcagaagtgt tagtggtcga tacccgtaat ggcaatattc tggcgaaggt
     3541 tgcagcaccg gaatcactgg ctgtgctgtt taacccagcg cgtaatgaag cctacgtaac
     3601 gcatcgtcag gcaggtaaag tcagtgtgat tgacgcgaaa agctataaag tagtgaaaac
     3661 gttcgatacg ccgactcatc caaacagcct ggcgctgtct gccgatggca aaacgctgta
     3721 tgtcagtgtg aaacaaaaat ccactaaaca gcaggaagct acccagccag acgatgtgat
     3781 tcgtattgcg ctgtaataga tatggcgagg gtgaaaacgg agagacttgc ctgactttta
     3841 tcacgccgca tgcggcacac cttcgtcagt aatctaaatt tgcccggcac gtagccgggc
     3901 aattttgcaa ttagctcgcc gtttcaggct taaacacaat ttcctgtttt tcttcatctt
     3961 cctcgacgac tggcgcagtg ctgtgaattt cagcaacgcg tttgcgcaca ccaaaccagc
     4021 ctataaccag cagaataccg ataatcggca gcgccgcgat agtataagtc ccgttcgggt
     4081 aatcgaacgc catcagcaca aggacactca gtaaaaacag taatgtcagc caggaagtga
     4141 agggcgcgcc aggcagttta aaactgacat ccgctgcttt gccttcttta atcgctttac
     4201 gcaggcgcat ctggcacacg atgataaacg cccatgaagc gatgattccc agcgacgcga
     4261 agttcaacac aatctcaaat acgcgcgacg gcaccagata gttgaggaat acgccgacga
     4321 catacacaac cagtgtcgcc agaatcccgg catacggcac atgctgacga ctcattttcg
     4381 ccataaaact cggtgcggaa ccgcccatcg ccatcgagcg cagaatacgc ccggtgcagt
     4441 acagacctga gttcaggctg gagagggcag cggtcagcac cacaatgttc ataatgctgc
     4501 cgatatatgg cacacccagt ttagagaaaa acgtcacgaa cggactttgc cccgcctgat
     4561 acgcgctcca cggcaataac ataaccagca acaccacgga gccgacgtaa aacaggccaa
     4621 tacgccaaat cacactgtta atggctttag gcaccatggt ctgcggatct ttacattctc
     4681 cggcagctgt acccaccatt tcaatggagg caaaagcaaa cactacgccc tgaatcaaca
     4741 ccagcgcagg cagcaaaccg tgggggaaga agccgccatt atcggtgatt aaatgaaagc
     4801 cagtggtgtt gccatccagc ggctgaccac taccgaggaa cactgtaccc acgaccaaaa
     4861 aggtcacaat ggcgagcact ttaataagcg caaaccagaa ctccatctcc gcaaaccatt
     4921 tcacaccgat catattcatg gtgccaacga tggtaagtgc agcgagcgca aagacccact
     4981 gcggcacgcc gccaaacgca ccccagtaat gcatatacag agcgacggcg gtaatatcaa
     5041 caatccccgt catcgcccag ttgatgaagt acatccagcc agcaacataa gcggctttct
     5101 cacccaaaaa ctcacgggca taagagacaa aactgccact ggaagggcgg tgtagcacca
     5161 gctcacccaa tgcacgcaga ataaaaaacg aaaacaagcc acaaattaaa taaaccagtg
     5221 ccagtgctgg acccgccatt tgcagtcggg ctcctgcacc taaaaacaag ccggtgccaa
     5281 tcgcgccgcc aatggcgatc atctgtacct ggcgattgcc catcgctttg tgatacccct
     5341 cttcgtgggc attaagccag cggcgtttcg cggcgtgttg atctgaagtg tcggtgtcgt
     5401 gtttactcat tgctctccct gattgcttta atgaaaaagt catataagtt gccatgaaca
     5461 atgtttattc atcctggcga tgatagttat caccattatt ctttatgggt ataaaactat
     5521 cggcgcagca tcctacccgt tatcgatata cgatgcaaaa catcccctta caatcctgaa
     5581 ggggattcat acaactgacg aaaaaatgac aaatactttt gctggttaac ctgtgtactg
     5641 tcctacactt aatctttaaa agattgtgag gggcatatga ttaaggtgta tggcgtaccc
     5701 ggatggggct ctacaatcag tgagctgatg ctaacactgg ctgatattcc ttatcagttc
     5761 gttgatgtca gtggttttga ccacgaggga acttcacgcg agttattgaa aaccctgaac
     5821 ccgttgtgtc agattccgac actggcgctg gaaaatgacg agattatgac ggagacagcg
     5881 gcgattgcat tgatggttct cgatcgtcgc ccggaccttg cgccaccggt tgggcgcgcc
     5941 gaacgtcagc agtttcaacg gctattggtc tggctggttg ctaatgtcta tccaacattc
     6001 actttcgccg attaccctga gcgttgggct cctgacgcac ctgaacagtt aaagaagaat
     6061 gttattgaat atcgtaaatc gctttatatc tggctgaatt cacaattgac cgctgaacct
     6121 tatgcgtttg gtgagcaact aacgctggtg gactgttatc tttgcactat gcgcacatgg
     6181 gggtctgggc atgaatggtt tcaggataat gccacgaata tcagtgcgat tgctgatgct
     6241 gtatgtcagt taccgaaatt acaagaagta ttaaaaagga atgaaattat ttgagtggga
     6301 ta
//
LOCUS       DAZOYL010000224         6256 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZOYL010000224 DAZOYL010000000
VERSION     DAZOYL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.225"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHUOY_003174"
     CDS             122..952
                     /locus_tag="ACHUOY_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR0683887.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUOY_003175"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUOY_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR0683888.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHUOY_003176"
     CDS             complement(2081..3541)
                     /locus_tag="ACHUOY_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR0683889.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUOY_003177"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUOY_003177"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR0683890.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..>6256
                     /gene="gntP"
                     /locus_tag="ACHUOY_003178"
     CDS             5146..>6256
                     /gene="gntP"
                     /locus_tag="ACHUOY_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR0683891.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISA"
BASE COUNT         1480 a         1619 c         1588 g         1569 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcac caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttaat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctg
//
LOCUS       DAZOYL010000225         6250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZOYL010000225 DAZOYL010000000
VERSION     DAZOYL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.226"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..510
                     /locus_tag="ACHUOY_003179"
     CDS             <1..510
                     /locus_tag="ACHUOY_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR0683892.1"
                     /translation="AGVFSFKDTLLMRMNNLNNDLVNYSHDNTRTSVGARLAMYEVGL
                     KTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDLIDTLSTRGIPGVVLTI
                     LAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKA
                     QSDQCLLDK"
     gene            complement(580..1551)
                     /gene="rfaC"
                     /locus_tag="ACHUOY_003180"
     CDS             complement(580..1551)
                     /gene="rfaC"
                     /locus_tag="ACHUOY_003180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR0683893.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(1555..2601)
                     /gene="rfaF"
                     /locus_tag="ACHUOY_003181"
     CDS             complement(1555..2601)
                     /gene="rfaF"
                     /locus_tag="ACHUOY_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR0683894.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2611..3543)
                     /gene="rfaD"
                     /locus_tag="ACHUOY_003182"
     CDS             complement(2611..3543)
                     /gene="rfaD"
                     /locus_tag="ACHUOY_003182"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR0683895.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3757..4953
                     /gene="kbl"
                     /locus_tag="ACHUOY_003183"
     CDS             3757..4953
                     /gene="kbl"
                     /locus_tag="ACHUOY_003183"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR0683896.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4963..5988
                     /gene="tdh"
                     /locus_tag="ACHUOY_003184"
     CDS             4963..5988
                     /gene="tdh"
                     /locus_tag="ACHUOY_003184"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR0683897.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT         1509 a         1548 c         1586 g         1607 t
ORIGIN      
        1 gcaggtgtat tttcttttaa agatacattg cttatgagaa tgaataactt aaataacgat
       61 ttagttaatt attcgcatga taacaccaga acttcagtcg gtgcccgtct ggcaatgtat
      121 gaagttggct taaaaacata ttctccaata ggacaatcac tggaaaaacg agcggaaaaa
      181 atacatgaac tagaagaaaa agagcctaga ttgagtggcg ctttacccta tatagattct
      241 catttgcata acgatctcat agatacgtta tcaacgcgtg gtattcctgg agttgtatta
      301 acaattttag ccttttcagc aatactcata tatgccttaa gaactgctaa agaaccttat
      361 attttaatct tgcttttttc actactggta gtaggcctaa gtgatgtaat actcttttct
      421 aaaccggttc cgactgctgt gtttgtcacc ataatattgc tttgtgctta ttttaaagca
      481 caatcagacc aatgtttatt agataaataa tgaatatgtg aaataaaatc agcaaggagg
      541 ctgattttat ttcaaaatta tctattaagg ggttgtaatt taaatcatag caacgttttc
      601 ttcaatgaat cgcttaacag catttgctgt caattgagac aactcattcc ccggagccct
      661 acaaaccatc tgattcttcc catatccacc aattaatccc ggatcggttg gtccataaac
      721 cgtgatattg ggtctatcca gtgccgccgt taaatggctt aaccccgtat ccaccgacac
      781 tacaaattta gccccggcca gcacgcgggc aacgccttcc agactcatct tcggcaatac
      841 ttcaacataa gcaaatcctt ccgccagtcg tttcgcccgc tcttcctcat gcggcgcgcc
      901 ccacggaagt ttaatccgta ttcctgaatc agccagtaaa ccaatcaatt ctcgccagtg
      961 ttcttccggc cagtgtttat catcacgggt cgtcgcatga agaaatacgg catattcgcc
     1021 agcatctgta ggcaggttcg tcaggaaatg ctgtgcaata gcataatcgc cctgggtttg
     1081 cggtttgcta tagcccagac ttttggcaaa cagttcgcgg gtgcgttcta cggcgtgctg
     1141 ctgttttgca atatgatgct tacgattgta aaacagactg gctaaaggtt cgcgagcggt
     1201 ttgccagtcc atgccatgct ttacgccatg cgccagacgc gtcaccagcg ccgcgctttt
     1261 taccagcccc tgagcgtcga taactgcgtc atagttctct gcttgtagcg cttcacgaaa
     1321 cgctttgcgt tccgctttta tgggggccga gaaccaggct ttacgccagc gacgtattgc
     1381 cacaggaata actcgctcaa cggcggcgtg ccaggaagga atctgtgcga acccttcttc
     1441 caccacccag tcaaacttaa tccctgggat tgcctgctgg gcatcagtga gtgcgggcaa
     1501 cgtatggaga acatcgccca tcgacgatgt tttaacgatc aaaacccgca tccgtcaggc
     1561 ttcctcttgt aacaatagcg cgttgagttc ttccagtacg cgctggggag taatgtcgat
     1621 taagctctgg tgataaccct ccgcagcgtc acctttacgc actttgtgat agccggtaat
     1681 caggcggatc acgcgcgctt tatgggatag cggcggtgtg aagtccgggc tactcggacc
     1741 atacagggca accagcggac gattgagcgc cgccgcaacg tgcatcaggc cagaatcgtt
     1801 agtgacaatg gctttacagg ctgcaatcag gataaccgct tgatcaagct gtgtttcccc
     1861 cgccaggttc cgacaccatg cctgctgctc ggtattcaaa gcggcaagaa tctcattgcc
     1921 cgcttcatga tctttcgccg agccaaacag aaccacctga taaccttcat caatcagctg
     1981 ctttgccagt tccgcatagt ggtagtgtgg ccagcgtttt gccggaccaa actccgcacc
     2041 cgggcaaaaa ccaatcatcg gacgttctga tgaaagcgaa aattgattac aggtatatga
     2101 tttttcacct tcgctcacct gcaattgcgg ccataacaac ggttttggta gatcttgtgc
     2161 ggtacgcatc acgcctttgt cataggccag cgcgacatag cgttccacca ttagcggcca
     2221 ggcttcttta tcgagcacgc gtaaatcgtt gagtaaaccg tagcgcatct cgccgcgcca
     2281 gccggtgcga tgaggaatac ccgcgaagaa aggcactaat gcagatttga aggagttggg
     2341 taagacgtag gcgcggtcgt agcgcttttc acgcaggcta tgacccagtt tgcggcgttc
     2401 gccgatttcc agcgctccgt gaccgagagg cataggaata gcttcgttaa cttccggcat
     2461 ccgcgataat aatggacggc accatgccgg tgccatcaca tcgattatcg cctggggata
     2521 gcgcgcctgg agcgtgcgat agagactttg cgacatcatc atgtcgccaa cccaagacgg
     2581 gccgatcacc agtattttca tgcagagctc ttatgcgtcg cgattcagcc aggccatgta
     2641 ttccgttaca ccttcagcaa cggttttgaa cggtttgtcg taacccgccg cgcgcagatt
     2701 tgtcagatct gcctgagtga acgcctggta gcggcctttc agcttatccg ggaacggaat
     2761 gtattcgatc tggcctttct tgtgataagc cagcgtagca tcagctacag cctggaagga
     2821 ttccgcacga ccagtaccga ggttgaagat gccggaaacg ccattttcca ggaaccacag
     2881 attcacatca gccacgtcgc ccacatagac gaagtcgcgt ttgaagttct cgctaccttc
     2941 aaacagcttc ggtgattcac cgttgttaag ctgggtgttg agatggaaag cgacgctcgc
     3001 catgctgcct ttatggcctt cacgcggtcc ataaacgttg aaatagcgga agccaacaat
     3061 ctgcgagtta gcttcaggca ggatttggcg aacatattca tcaaacagga attttgagta
     3121 accgtagacg ttcaacggtt tttcgtactc gcgggattca ataaagtcgg aggtgcgtcc
     3181 gccgtaggtg gctgcggaag aggcatacag gaacgggatt tcgcgctcca gacagtagtg
     3241 cagcagctct ttggagtatt gatagttgtt atccatcata tacttgccat cccactcggt
     3301 ggtggaagag cacgcgcctt cgtggaaaat cgcttcgaca tcgccgaact cttcgccagc
     3361 cataatctgg atcaggaagt cttccttatc catatagtcc gcgatatcca gatccaccag
     3421 gttcacaaac ttggtgccgt ctttcaggtt gtccaccacc agaatatcgg tgatgccttt
     3481 atcattcagg gccttaacga tgttgctgcc gataaagccc gcgccgccgg taacgatgat
     3541 cataactgta accttcgaat tatggagtca gagacaatct cagacacgaa tacttctatc
     3601 atatcactac acggcgttgg cttcagccat tcgccaaaag tgagattgat ggacgttatt
     3661 tatcctgaat tttgcagaag tgttaacgcg tcatctcgtc gcgacctata agtttgggta
     3721 atatgtgctg gaatttgccc tgtctggaga atcgcaatgc gtggagaatt ttatcagcag
     3781 ttaaccaacg atctggaaac cgcacgggcg gaagggttgt ttaaagaaga gcgcattatt
     3841 acgtctgcgc agcaagcaga tatcactgtg gctgatggaa gccacgtcat taacttttgt
     3901 gccaataact atctcgggct ggcgaattat ccggatctga ttgcggcggc aaaggcggga
     3961 atggattctc acggtttcgg catggcttcg gtgcgtttta tttgcggcac tcaggacagc
     4021 cataaagagc ttgaacaaaa actggcggca ttcctgggga tggaagatgc gattctctac
     4081 tcttcctgct ttgatgctaa cggtggcctg tttgaaacgc tgctgggcgc ggaagacgcc
     4141 attatctccg acgcactgaa ccacgcgtct attattgatg gtgtgcgtct gtgtaaagct
     4201 aaacgctatc gctatgccaa caacgatatg caggagctgg aagcacgtct gaaagaagcg
     4261 cgtgaagccg gtgcgcgtca tgtgctgatt gccaccgatg gtgtgttctc gatggacggc
     4321 gtgattgcca acctgaaggg cgtttgcgat ctcgcagata aatatgatgc cctggtgatg
     4381 gtagacgact cccacgcggt cggttttgtc ggtgaaaatg gtcgtggttc ccatgaatac
     4441 tgcgatgtga tgggccgggt cgatattatc accggcacgc tcggtaaagc gctgggcggg
     4501 gcttctggtg gttataccgc ggcgcgcaaa gaagtggttg agtggctgcg ccagcgttct
     4561 cgtccgtacc tgttttccaa ctcgctggca ccggccattg ttgccgcatc catcaaagta
     4621 ctggagatgg tcgaagcggg tagcgagctg cgtgaccgtc tgtgggcgaa cgcgcgtcag
     4681 ttccgtgagc aaatgtcggc ggcgggcttt accctggcgg gagccgatca cgccattatt
     4741 ccggtcatgc ttggtgatgc ggtagtggcg cagaaatttg cccgtgagct gcaaaaagag
     4801 ggcatttacg tcaccggttt cttctatccg gtcgttccga aaggtcaggc gcgtattcgt
     4861 acccagatgt ctgcggcgca tacccctgag caaattacgc gtgcagtaga agcattcacg
     4921 cgtattggta aacaactggg cgttattgcc tgaggatgtg agatgaaagc gttatccaaa
     4981 ctgaaagcgg aagagggcat ctggatgacc gacgttcctg taccggaact cgggcataac
     5041 gatctgctga ttaaaatccg taaaacagcc atctgcggga ctgacgttca catctataac
     5101 tgggatgagt ggtcgcaaaa aaccatcccg gtgccgatgg tcgtgggcca tgaatatgtc
     5161 ggtgaagtgg taggtattgg tcaggaagtg aagggcttca agattggcga tcgcgtttct
     5221 ggcgaaggcc atatcacctg cggtcactgt cgcaactgtc gtggcggtcg tacccatctg
     5281 tgccgcaaca cgatcggcgt cggcgttaac cgcccgggct gcttcgccga atatctggtg
     5341 attccggcgt tcaacgcctt caaaattccc gacaatatct ccgacgacct ggcttccatt
     5401 tttgatccct tcggtaacgc cgtgcatacc gcgctgtcgt tcgatctggt tggcgaggat
     5461 gtgctggtct ctggtgcagg cccgattggt attatggcag cggcggtggc gaaacacgtt
     5521 ggtgcacgca atgtggtgat cactgatgtt aacgaatatc gtctcgaact ggcgcgcaaa
     5581 atgggtatca cccgtgcggt caacgtcgct aaagaaaacc ttaatgatgt gatggcagaa
     5641 ctgggcatgg ccgaaggctt tgatatcggt ctggaaatgt ccggtgcgcc gccagcgttt
     5701 cgtaccatgc tcgacaccat gaaccacggc ggtcgcattg cgatgctggg tattccaccg
     5761 tccgatatgt ctatcgactg gaccaaagta atctttaaag gcttgttcat taaaggtatt
     5821 tacggtcgtg agatgtttga aacctggtac aagatggcgg cgctgattca gtcaggtctg
     5881 gatctctcgc cgatcattac ccatcgtttc tctatcgatg atttccagaa gggctttgac
     5941 gctatgcgtt cgggccagtc cgggaaagta attctgagct gggattaaca cgaacaaggg
     6001 ctggtattcc agcccttttg cctgaggata atccgttaaa tatgtaaaat cctgtcagtg
     6061 taataaagat atcgtaattg cgctgatctc ttatatagct gctctcatta tctctctacc
     6121 ctgaagtgac tctctcacct gtaaaaataa tatctcacag gcttaatagt ttcttaatac
     6181 aaagcctgta aaacgtcagg ataacttcag aggtcgtcgg taatttatga tgaacagcac
     6241 caataaactt
//
LOCUS       DAZOYL010000226         6222 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZOYL010000226 DAZOYL010000000
VERSION     DAZOYL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.227"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..934
                     /gene="phoE"
                     /locus_tag="ACHUOY_003185"
     CDS             <1..934
                     /gene="phoE"
                     /locus_tag="ACHUOY_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0683898.1"
                     /translation="HYMSDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNK
                     AESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNF
                     MTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSD
                     FAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITG
                     GFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKN
                     MSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
     gene            complement(973..1374)
                     /gene="crl"
                     /locus_tag="ACHUOY_003186"
     CDS             complement(973..1374)
                     /gene="crl"
                     /locus_tag="ACHUOY_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0683899.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1432..2676)
                     /gene="frsA"
                     /locus_tag="ACHUOY_003187"
     CDS             complement(1432..2676)
                     /gene="frsA"
                     /locus_tag="ACHUOY_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0683900.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2768..3226)
                     /gene="gpt"
                     /locus_tag="ACHUOY_003188"
     CDS             complement(2768..3226)
                     /gene="gpt"
                     /locus_tag="ACHUOY_003188"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0683901.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3487..4944
                     /gene="pepD"
                     /locus_tag="ACHUOY_003189"
     CDS             3487..4944
                     /gene="pepD"
                     /locus_tag="ACHUOY_003189"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0683902.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5001..5615)
                     /gene="prfH"
                     /locus_tag="ACHUOY_003190"
     CDS             complement(5001..5615)
                     /gene="prfH"
                     /locus_tag="ACHUOY_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0683903.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5612..6222)
                     /locus_tag="ACHUOY_003191"
                     /pseudo
     CDS             complement(5612..6222)
                     /locus_tag="ACHUOY_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
BASE COUNT         1571 a         1611 c         1533 g         1507 t
ORIGIN      
        1 gcattatatg agtgataacg ccagtaaaga tggcgaccag agttatatcc gttttggttt
       61 taaaggtgaa acacaaatta acgatcaact gactggttat ggtcggtggg aagcggagtt
      121 tgccggaaat aaagcggaga gtgatactgc acagcagaaa acgcgtctcg cttttgccgg
      181 gttgaaatat aaagatttgg gttctttcga ttatggtcgt aacctgggcg cgttgtatga
      241 cgtggaagcc tggaccgata tgttcccgga atttggtggc gattcctcgg cgcagaccga
      301 caactttatg accaaacgcg ccagcggtct ggcgacgtat cggaacaccg acttcttcgg
      361 cgttatcgat ggcctgaact taaccctgca atatcaaggg aaaaacgaaa accgcgacgt
      421 taaaaagcaa aacggcgatg gcttcggcac gtcattgaca tatgactttg gcggcagcga
      481 tttcgccatt agtggggcct ataccaactc agatcgcacc aacgagcaga acctgcaaag
      541 ccgtggcaca ggcaagcgtg cagaagcatg ggcaacaggt ctgaaatacg atgccaataa
      601 tatttatctg gcaactttct attctgaaac acgcaaaatg acgccaataa ctggcggctt
      661 tgccaataag acacagaact ttgaagcggt cgctcaatac cagtttgact ttggtctgcg
      721 tccatcgctg ggttatgtct tatcgaaagg gaaagatatt gaaggtatcg gtgatgaaga
      781 tctggtcaat tatatcgacg tcggtgctac gtattatttc aacaaaaata tgtcagcgtt
      841 tgttgattat aaaatcaacc aactggatag cgataacaaa ttgaatatta ataatgatga
      901 tattgtcgcg gttggcatga cctatcagtt ttaatggata ttgccggatg tgatacatcc
      961 ggcacatttc actcacgccg ttaacttcac cggttcatca cgaaaatcat ccgccggttc
     1021 cagcttcaga ttcagcgtcg tcagcagttc acgcagcttc tcgtgaaact cacgtagggt
     1081 gtgctccagt cgttcaacca cttcagtatc ttttaccgga acactcttcc agtcgcctgc
     1141 tttatcgaac agaccaaact ggtaactgta ggtaaaacgg gattcctgcg cttcaagctc
     1201 catccaccag ccccagaact cacgcacttc cggtgccggt ttcacgttga cgcatacagc
     1261 cagacaatcg aaaaagaatc gattatcttc gcacttacct tcacgaatat acgggcctag
     1321 tgcggtaaat tttttgatca atctgctctt cgggtgtcca ctcggtaacg tcattgcaat
     1381 ctcctgttgt gatgcaactg ttttaccaaa ttggcaaatt ttagcaaatt tttaacacaa
     1441 gcgtttttcg atccagccgg tgatttcctg aagacctttg tcaaaattcc gatacaccgg
     1501 gttaaatggg atctctaata atttaccgtc agcagatgat gaggtgatta agcgtgagtc
     1561 ctcttccggg ctgaacggat cgttcttcca gtagcctgat aacattggcg ttgggcagcg
     1621 acgccccagc aatccttgca cttttaatga atagcgattc agctccacgc gtaacgcttc
     1681 gtcggaagca tcatgcatcc ccaaacgact cgccagaacg tcaagataca tttccggcac
     1741 ctgttgctgg cacttaaaat cactcaacag ggtatgaact accggaccaa gacaggcaac
     1801 cgctttcaga cgcggcgatt caagatatgc cagacgcacg gcaacgttag cgccgaaacg
     1861 gaaaccaaag gccgcgacgc gagtgtgatc cacccacggt acgttaggca gagcctttaa
     1921 aacgtgctga tgcaacaggc tggagtcctg ggtgagcttc cattttgatg aaaagcccac
     1981 cgacggcata tcaatagtca gcatcgcaat gccgcgcggc gcaaaataac gttcatacag
     2041 gctgtaatag tccgtctgca tcgcatccag accaccacac attaatactg tcgggaacgg
     2101 gccatcgcct ttcggcatat gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa
     2161 ctccatctgc cgcatcgtgc ccggcagacg ctgggcggct tcttcatagg cacggtttga
     2221 caaagcctgc gcttgctcgg ccaggtcatc tcctttcaga tgaggatagg cagcaatgtt
     2281 gtacaacgta gccgcatgca gccagtgacg accactgagt tgcggatctt cttccgcaca
     2341 ggctttttgt tgccacacca tcgcctggat ggcccactca taaatccagt tgccaccacg
     2401 gtagccaatc accgtatcgt ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat
     2461 acgcgcctgg acgtcgagga tttcacgtgg gtcaatgccg cgccagatcc acatcagacg
     2521 gttaatcatg cgataccagt gagggatggt tttaccatca agggccgact gcacaggcgg
     2581 ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg
     2641 tttgaacagg gtttcgctca ggtttgcctg tgtcatggat gcagcctcca gaatacttac
     2701 tggaaactat tgtaacccgc ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt
     2761 gaaaagatta gcgaccggag attggcggga cgaatacgac gcccatatcc cacggctgtt
     2821 caatccaggt atcttgcggg atatcaacaa catagtcatc aaccagcgga cgaccagccg
     2881 gttttgcgaa gatggtgaca aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag
     2941 taccaccggt atccaccagg tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc
     3001 gttttagcac tttaagctcg cgctggttgt cgtgatcgta gctggaaata caaacggtat
     3061 cgacatgacg aatacccagt tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc
     3121 ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg
     3181 catggatctg caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag
     3241 cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga
     3301 gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc
     3361 tccctgacgc gggataaagt ggtattctca aacatatctc gcaagcctgt cttgtgttga
     3421 caacattttc tgctaaccct gtgacctgca atactgtttt gcgggtgatc gacaaggaga
     3481 cttaacgtgt ctgaactgtc tcaattatct ccacagccgc tgtgggatat ttttgccaaa
     3541 atctgttcta ttcctcaccc gtcctatcat gaagagcaac tcgctgaata cattgttggt
     3601 tgggcaaaag agaaaggttt ccatgtcgaa cgcgatcagg taggtaatat cctgattcgt
     3661 aaacctgcta ccgcaggtat ggaaaatcgt aaatcggtcg tcttacaggc ccacctcgat
     3721 atggtgccgc agaaaaataa cgacaccgtg catgacttca cgaaagatcc tatccagcct
     3781 tatattgatg gcgaatgggt taaagcgcgc ggcaccacgc tgggtgcaga taacggcatt
     3841 ggtatggcgt ctgcgctggc ggttctggct gacgaaaacg tggttcacgg cccgctggaa
     3901 gtgctgctga ccatgaccga agaagccggt atggacggtg cgttcggctt acagagcaac
     3961 tggttgcagg ctgatattct gattaacacc gactccgaag aagaaggtga aatctacatg
     4021 ggttgtgcgg ggggtatcga cttcacctcc aacctgcatt tagatcgtga agcggttcca
     4081 gctggttttg aaaccttcaa gttaacctta aaaggtctga aagggggtca ctccggcggg
     4141 gaaatccacg ttggcctggg taatgccaac aaactgctgg tgcgcttcct agcgggtcat
     4201 gcagaagaac tggatctgcg ccttatcgat ttcaacggcg gcacactgcg taacgccatc
     4261 ccgcgtgaag cctttgcgac cattgctgtc gcagctgata aagtcgacgt cctgaaatct
     4321 ctggtgaata cctatcagga gatcctgaaa aacgagctgg cagagaaaga gaaaaatctg
     4381 gccttgttgc tggactctgt agcgaacgat aaagctgccc tgattgcgaa atctcgcgat
     4441 acctttattc gtctgctgaa cgccaccccg aacggtgtga tccgcaactc agacgtggca
     4501 aaaggtgtgg tcgaaacctc cctgaacgtc ggtgtggtga ccatgactga taataacgta
     4561 gaaattcact gtctgatccg ttctctgatc gacagcggta aagactacgt ggtgagcatg
     4621 ctggattcgc tgggtaaact ggctggcgcg aaaaccgaag cgaaaggcgc atatcctggc
     4681 tggcagccgg acgctaattc tccggtgatg catctggtac gtgaaaccta tcagcgtctg
     4741 ttcaacaaaa cgccgaacat ccagattatc cacgcgggcc tggaatgtgg tctgttcaaa
     4801 aaaccgtatc cggaaatgga catggtttct atcgggccaa ctatcaccgg tccacactct
     4861 ccggatgagc aagttcacat cgaaagcgta ggtcattact ggacactgct gactgaactg
     4921 ctgaaagaaa ttccggcgaa gtaattattt gatttgctgc cggatggcgt ttaatcgcct
     4981 tccggcagtt tcatccttca ttatccttcg ataaaagcca tccctgtaaa tgtccgtctc
     5041 gggttgccac gttcaatctg gtgatggaac attcgccgct gcgatttcag caccgcgcta
     5101 ttttcctgtt gctgctgctc cagcttccag gcaatcagca atcgtgccag ccgcttgtta
     5161 gcatgctgac tacgctctga ctgaaccttc acgctaatac cggatgccaa atgcgtggcg
     5221 cgtaccgccg agtcggtttt attgacatgt tgaccgcccg gccccgacga acgcaacgtc
     5281 tcataacgga ttgcatccga ttgttcctgc tcatcagcgg taaaacgccc aatgcccaga
     5341 aaccagtttt tgcgcccatg atgaggccga tacggactcg gacaaatcca ctgaatagtg
     5401 ccgcaccagc tttcgcttaa tgcccatgcg ttatcgccat ccagagaaac cagagctgaa
     5461 cgcagcgtgt cagagtagcg gcccgtttct gtttccaaca ccgttaccgc gacgtcttgt
     5521 cgggcagctt ctttaattaa tcgctccagc gcctttttca ctgcgagaca acattcttcc
     5581 ggcccctgcg ctgaggagag ttgtagcaag atcatccttt tttccctcca ctgtttttga
     5641 gcgttagcac cggacgcagc cgcgcgacag gaataattaa accagcctgc accagacatt
     5701 gcaccacgct ttcagccgat ttataggctt gtggcgcttc ttcaaagatg agttgtttat
     5761 cgcgacaaat tacccgactg ccaagttcag tacgtgagag ctgcgtcgct gtgtatttcg
     5821 ctgccagacg ccctttacac tcggtgcgcc cccatttacg cccagcccca tgcgccagcg
     5881 agtggagcgt tttttcgttc gcaacaggct gtacaagcta ggagtaatca ccgagtgaac
     5941 cgggaataat caccagaccg ttgtcatccg gcgtggcacc tttacgatgc aaccagccct
     6001 gttgatcgcc gatttgacac gcgctaacaa agttatgcgc cacatccaga accggacttc
     6061 cggtggcctt aacctgttgc attatgcgta aagagatcag ctggcgatta atacgcgcaa
     6121 acgccagcgc atcgtcatgt tccgcaatat accgtagcgc gtcgtcactg ccttcaggca
     6181 aaccatgatg cgaaaacgag gcaatatgcc gctgtaaaat ag
//
LOCUS       DAZOYL010000227         6111 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZOYL010000227 DAZOYL010000000
VERSION     DAZOYL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.228"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            3..1391
                     /locus_tag="ACHUOY_003192"
     CDS             3..1391
                     /locus_tag="ACHUOY_003192"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0683904.1"
                     /translation="MHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLIGGEQQIAD
                     LQQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLETVLAAMCEPQIAIVSLTITE
                     KGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALARRKAAGLPAFTVMSCDN
                     MPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQLTG
                     VGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLA
                     YLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLKVQGVDLQDYANRLIERYSNPA
                     LRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVDEQGNPI
                     EISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTAKVTEAYLSLLAH
                     GAKATVAKYSVK"
     gene            complement(1427..1630)
                     /gene="ydfZ"
                     /locus_tag="ACHUOY_003193"
     CDS             complement(1427..1630)
                     /gene="ydfZ"
                     /locus_tag="ACHUOY_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR0683905.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1807..2493)
                     /gene="rspR"
                     /locus_tag="ACHUOY_003194"
                     /gene_synonym="ydfH"
     CDS             complement(1807..2493)
                     /gene="rspR"
                     /locus_tag="ACHUOY_003194"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR0683906.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2582..3328)
                     /gene="ydfG"
                     /locus_tag="ACHUOY_003195"
     CDS             complement(2582..3328)
                     /gene="ydfG"
                     /locus_tag="ACHUOY_003195"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR0683907.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3465..5510
                     /gene="dcp"
                     /locus_tag="ACHUOY_003196"
     CDS             3465..5510
                     /gene="dcp"
                     /locus_tag="ACHUOY_003196"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR0683908.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5555..6073)
                     /locus_tag="ACHUOY_003197"
     CDS             complement(5555..6073)
                     /locus_tag="ACHUOY_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR0683909.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1511 a         1465 c         1583 g         1552 t
ORIGIN      
        1 ttattcattt aggctttggt gcatttcacc gtgcgcatca gggcgtgtat gccgatattc
       61 ttgctacaga acatttcagt gactggggat attatgaggt caacttaatc ggcggcgaac
      121 agcaaattgc cgatttacaa cagcaagata atctttatac cgttgcggaa atgtcggccg
      181 atgcgtggac ggctcgcgtc gttggcgtcg ttaagaaagc cttgcacgta cagatggatg
      241 gcttagaaac cgtgttggct gcgatgtgtg aaccgcaaat cgcgattgtc tctctgacaa
      301 tcaccgaaaa agggtatttc cactctccgg cgaccggaca gttaatgctc gatcacccga
      361 tggtcgctgc cgacgtgcaa aatccccacc agccgaaaac agcaacaggg gtgattgttg
      421 aggcgctggc ccgccgtaaa gcggcaggac ttcccgcatt taccgtcatg tcatgtgaca
      481 acatgccaga aaacggtcat gttatgcgtg acgttgtcac ttcatacgcg aaagctgttg
      541 atgtaaaact ggcacaatgg atcgaagata acgtgacttt cccatcaaca atggtggacc
      601 gtattgtgcc cgcagtgaca gaggatacgc tggcgaaaat cgaacaactt accggtgtgg
      661 gcgatcctgc gggcgttgcc tgtgaacctt tccgccagtg ggtaatagaa gataactttg
      721 ttgccggacg tccagaatgg gaaaaagcgg gagccgaact ggttagtgat gtgctgcctt
      781 atgaagagat gaagttgcgc atgctcaacg gcagtcattc attcctggcg tatttgggtt
      841 atcttgctgg atatcagcac attaatgact gcatggaaga tgaacattat cgttatgcgg
      901 cgtatggctt gatgttgcag gaacaagcgc cgacgctgaa agtgcagggc gttgatttgc
      961 aagattacgc taaccgatta attgaacgct atagcaaccc ggcgttacgt catcgaacct
     1021 ggcagattgc gatggatggc agccagaaat tgccacagcg gatgttggat tctgttcgct
     1081 ggcatctggc gcatgacagc aagttcgatc tgctggcact gggcgtcgcg ggttggatgc
     1141 gttatgtcgg tggtgttgat gaacagggaa atccgattga aatcagtgac ccactgttac
     1201 ctgttattca gaaggctgta caaagtagtg ctgaagggaa agcgcgcgtc cagtcattgc
     1261 tggcgattaa ggcgatcttt ggtgatgatt tgccagacaa tagtttgttt actgcaaaag
     1321 tgacggaagc gtacttgtct ttattagcgc atggcgcgaa agcgaccgtg gcgaaatatt
     1381 ccgtgaagta aaaaaagcaa tgccgccatc ggcggcattc acacgcttag ttcatgccga
     1441 gacgaatcag gtccagaggt gccagtttct cttcacaacc ttcaacaact acggtttttc
     1501 cgcggcggat ttgctcagcg gtcagacctt cagtatcaat cgacaggatc ttgccagtgt
     1561 gaccggtgcc gctaaccata acacggctgc cagtggtgat tgcgttacgg ttacgatcgt
     1621 aggtcgtcat aggtattttc tcctttctga tttacagtcg aagcccacgt aacgggcgca
     1681 atataaatac tcctgtcgag tgaaatattt tttgtttttg atcaagctca catctttttt
     1741 gatacatgca tgagccataa ggcgggggaa aagactctca ccccggatgg gatgagagga
     1801 aatgaattac tcttcgctaa accagtcgct attttcctgg cggatctggc gcacggattc
     1861 gctgatttcc tgcaaatgtt gcgtcattgc acgttctacc gcatcgccat cacgtttttg
     1921 cagggcagag aaaatatcaa gatgctggcg taacagcatt tctggtggag aaacgtggtc
     1981 gaaactcata tagcgcacgc gatcaacggt cgctttcagg ttctcaatgg tatcccacgc
     2041 cagttgacag tcggcaatct gcgtcaggag ttgatggaag ttgtcatcaa gttcaaaaaa
     2101 atcatccagt tgcttgcgct caatggcaat gcgttgctgg tgaagatttt gttccagttg
     2161 gtagcactgg ctttcggtaa tcatgctcgc cgcccgacgc gccaccgcgc actcaatggc
     2221 ctgacggata aaactgccat tgcgcacctg ggccatggaa attttgttga cgtagctgcc
     2281 acgctgcgga cgaatttgaa tcaggccgtt ttccgccagt ttaataaagg cttcacgaac
     2341 cggctggcgt gacacattga aacgaacaga aacttctttt tccgacaacg gtgtgcctgg
     2401 tgcaatcagg caatggacaa tgtcgcgacg aagaatacga taaatttgct gattgacagg
     2461 ctgtgtggga ttaagttgcg tttcgacggt cattcgttct tgcttgtgag tgagttaact
     2521 gcatgagtct accacttttt tgtgacaagc aataacccgg cagttacgcc gggtataaaa
     2581 attactgacg gtggacattc agtccggcat agctttgggt aaccggcatc atttccaggg
     2641 tattgatatt gacgtgagca ggcagcgttg acacccacca gacggcttcg ctgacatctt
     2701 ctggcgtcaa tgcaacggta ttttgatagg ttttttccgc tttaccgtca tcgcctttaa
     2761 agcggacatt ggaaaactcg gtgccaccca ccagacccgg ttcgatgtcg gtgacgcgca
     2821 ccgccgtacc atgcaggtcc gtacgcagat tcaggctaaa ctgacgaaca aacgctttcg
     2881 tcgcaccgta aacgttacca ccggcatacg gccagctacc tgccgttgag ccaatgttaa
     2941 taatatgacc atgattacgt tcaaccatac ccggtaagac ggcgcgcgtc atatatacca
     3001 ggcctttgtt gttggtatca atcatcgttt cccagtcttc aacgctggct ttatgcgcag
     3061 gctccatgcc caacgccagg ccggcattat ttaccaggat atcaatattg caccactcgg
     3121 caggaagcga tgccagcatc tcttcaatag cggcgcggtt gcgaacgtcc agttgggcga
     3181 tatacagatt atctcccagt tcgtctttta actcctgcaa ccgctcctgg cgacggccag
     3241 tggcgataac tttatgccct tgttgaataa aacgacgagt aatgcattca ccaaaacctg
     3301 ccgttgctcc agttactaaa acgatcatct cactgttcct caacgctttt gtgtgtcaat
     3361 aacatagcac gtgatgctta agagcgggta atactggttt gcaagattgc actccgttat
     3421 cagtaagcct acgctatgga acatctcgcg ttcaggagaa gaaaatgaca acaatgaatc
     3481 ctttccttgt gcaaagcaca ctaccgtatc tggctcccca ttttgatcaa attgccaatc
     3541 atcactatcg cccggcattc gatgagggaa tgcagcaaaa gcgggcagaa attgctgcca
     3601 tcgcgcttaa cccgcaaacg cctgatttca acaatactat tctggcactg gaacaaagcg
     3661 gagaattact tacccgcgtt accagcgtct tttttgcgat gactgcggcg cataccaatg
     3721 atgaattaca gcgtcttgat gaacagtttt ccgctgaact ggcggaactg gctaatgata
     3781 tctatctgaa cggtgaatta ttcgcgcggg tagatgctgt ctggcagcgc cgtgaatccc
     3841 tggggcttga tagtgaatcc atccgcctgg tggaggtgat tcatcaacgt tttgtccttg
     3901 ccggagccaa acttgcgcaa gctgataaag caaaattaaa agtactgaat acagaagctg
     3961 cgaccctgac cagccagttt aaccagcgat tactggcagc aaataaatcc ggcggtctgg
     4021 ttgtgaacga tatcgcgcag ctggcaggaa tgagtgagca agagattgcg ctggcggcag
     4081 aggcggctcg cgagaaaggt ctggataaca aatggctgat tccgctgctg aataccaccc
     4141 aacaaccggc gcttgccgaa atgcgcgatc gtgcgacgcg tgaaaaactg tttattgcgg
     4201 gctggacgcg agcggaaaaa aatgatggca atgatacccg cgctatcatt caacgtctgg
     4261 tagagattcg cgcacagcag gcgaaactgc ttggttttcc tcattatgcc gcatggaaaa
     4321 tcgccgatca gatggcaaaa acgccagaag cagcactcaa ctttatgcgg gaaattgttc
     4381 cagcggcgcg tcaacgtgcg agcgatgaat tagcctccat acaggcggtt atcgataagc
     4441 agcagggcgg gtttagcgcg cagccgtggg actgggcatt ttatgcggaa caggtacggc
     4501 gggagaaatt tgatcttgat gaagcgcagc tcaagccata ttttgaatta aacacggtgt
     4561 tgaatgaagg tgtattctgg accgcgaatc agctcttcgg tattaagttt gtcgaacgtt
     4621 ttgatattcc tgtctaccat cctgacgttc gggtgtggga aatttttgat cataatggcg
     4681 tggggctggc gttattttac ggtgatttct tcgcccgtga ttcaaaaagc ggcggtgcat
     4741 ggatgggcaa ttttgttgag caatcaacgc ttaatgaaac gcatccggta atttataacg
     4801 tctgcaatta tcagaaaccc gctgccggtg agcctgcgtt gttactctgg gatgatgtaa
     4861 taaccttatt ccatgaattt ggtcatacgc tgcacggcct ttttgcccgc cagcgttatg
     4921 ccacgctttc cggcaccaac acgccgcgtg attttgtcga atttccgtcg caaatcaacg
     4981 aacactgggc aacgcatccg caggtattcg ctcgctacgc ccggcattat cagagcgggg
     5041 cagcaatgcc tgacgaactg caacagaaaa tgcgtaatgc cagcctgttc aacaaagggt
     5101 atgagatgag cgaactgctt agcgccgcac ttctcgatat gcgctggcat tgcctggaag
     5161 aaaacgaagc aatgcaggat gtcgatgatt ttgaattgcg ggcgctggtg gcggaaaata
     5221 tggatcttcc tgctataccg ccacgctatc gcagcagtta tttcgcccat atttttggtg
     5281 gcggatatgc tgcaggttat tacgcttatc tgtggacgca aatgttggcc gatgatggtt
     5341 accagtggtt tgttgagcag ggcggattaa cgcgtgaaaa tgggcagcgt tttcgcgagg
     5401 cgatcctttc cagaggtaac agcgaagatc tggaacgcct gcatcgacaa tggcgcggta
     5461 aggctcctca gattatgccg atgctgcaac atcgtggctt gaacatataa acaccaccaa
     5521 tggctggcct ggggatatcg ccaggccagc ctggttatag cagtaattgc agcagctggg
     5581 cgagggcaaa cctcaccgct aaagctaata ccgtttcgca gtcgccagca aaatgcataa
     5641 ccgcagtgta gttctggcct ttaatatgcc acgcgaacca gacggtaccc gctggcgtac
     5701 catcttcacc gccctccggt ccgccgtagc cggtaatggc aatactgaca tcagcatccg
     5761 cacgctctat ggcacctgtt gccatttctg ctgccacttt ctcactcacc gcagaatatc
     5821 gttcaagaga ctgctggctt acgctgagga ttttcatctt tgcctgatcg gtgaaagtaa
     5881 caaagcctgc accgtaaaat ttgggtgtat cttcagctgc acacagggcg ctagccaact
     5941 taccgccggt gcatgattcg gcggtagtta agcgtaattg atccgcgata agtctttgac
     6001 tcaacgcgga tgtcaggttt tcaatagtgt cggtatctgc gagttgtaca attttatctt
     6061 tattaatgtt cactgttttt tgtccacgct attatatgta aaggtgattt g
//
LOCUS       DAZOYL010000228         6099 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZOYL010000228 DAZOYL010000000
VERSION     DAZOYL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.229"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            170..898
                     /gene="gadW"
                     /locus_tag="ACHUOY_003198"
     CDS             170..898
                     /gene="gadW"
                     /locus_tag="ACHUOY_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR0683910.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1261..4374)
                     /gene="mdtF"
                     /locus_tag="ACHUOY_003199"
     CDS             complement(1261..4374)
                     /gene="mdtF"
                     /locus_tag="ACHUOY_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR0683911.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4399..5508)
                     /gene="mdtE"
                     /locus_tag="ACHUOY_003200"
     CDS             complement(4399..5508)
                     /gene="mdtE"
                     /locus_tag="ACHUOY_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR0683912.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5616..5894
                     /locus_tag="ACHUOY_003201"
     CDS             5616..5894
                     /locus_tag="ACHUOY_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683913.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5895..>6099)
                     /gene="gadE"
                     /locus_tag="ACHUOY_003202"
     CDS             complement(5895..>6099)
                     /gene="gadE"
                     /locus_tag="ACHUOY_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR0683914.1"
                     /translation="HKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKF
                     NIMYKLKLRRMSDIVTLGITSYF"
BASE COUNT         1503 a         1507 c         1529 g         1560 t
ORIGIN      
        1 tattcgtgct gcatttactt attatcaatt aactgttatg caaaactact ttgtggataa
       61 attttggtcc taccaaatct ggcagttttt tcgctaagaa acagtctggc aacatttcat
      121 tagtatactg aaattgaaat aatcgcagta tgaaatataa gggataatca tgactcatgt
      181 ctgctcggtg atcctcattc gtcgttcatt cgatatttat catgaacagc ataaaatatc
      241 gctgcataac gagagtatcg tgctgctgga gaaaaatttg gcagacgatt ttgcgttttg
      301 ttcaccggat acgcgacgac tggatatcga tgagctgaca gtttgccatt acttacaaaa
      361 tattcgtcag ctaccacgca atttagggtt acatagcaaa gaccgtctat taattaacca
      421 gtcacccccc atgccgctgg tgacggcgat ttttgatagc ttcaatgaat ccggggtaaa
      481 ttcaccgata ctgagcaata tgctctacct ttcctgttta tcgatgtttt ctcataagaa
      541 agaactgatc cccttacttt tcaatagcat cagcactgtt tcaggaaaag ttgaacgcct
      601 tattagcttt gatatcgcca aacgttggta tctgcgcgat atcgcggaaa gaatgtatac
      661 cagcgagagt ctcataaaaa aaaagttgca ggatgaaaat acctgtttca gtaaaatatt
      721 actcgcttcc aggatgtcga tggccagacg attactcgag ttacgtcaaa ttcctctgca
      781 tactattgca gaaaaatgtg gctatagcag tacgtcgtac tttataaaca catttcgaca
      841 atattatggt gtaacgccac atcagtttgc gcaacattcg ccaggtacct tttcctgaca
      901 tattttgcat ttgaatattg gtcaggatct cacacctgct tcatgtgaaa ctcttccctg
      961 atgatttctg ccgggctacc ggctagttct ctttcgcagt ttgccaggcg gtataggtat
     1021 tttcaggagg aaaagactcg gcatgtttgg gattattaag ctgacaattc atacgattac
     1081 gggaatgtgg gtcagtattg tgctgttcaa attgatgacg aatggctgga gtggtttcta
     1141 ctttcagtgc tgtgtactaa gcctggtatt cctgacggtt agctggttgt tgagtggtga
     1201 atggttagca ggaaagagta aggctgaacc ttcacgttca accttactct catttacacg
     1261 ttacgctttt ttaaagcggg caaagagatg ttccactaca acgaaaaaga ccggaacgaa
     1321 gtaaattgcc agcactgttg cggcaaacat cccgcccatc acgccggtac ctaccgcgtt
     1381 ttgcgcgcca gaaccggcac catgactgat aaccagcggc agcacgccga gaataaaggc
     1441 gagagaggtc atcaggattg ggcgtaaacg catccgcgcc gcttcgatga ttgcctctat
     1501 cggcgttttc ccttctttct gcatcatctc aacggcaaat tcgacaatca ggatggcgtt
     1561 cttggcggaa agcccgatgg tggtcagcaa accaacctgg aagtagacgt cattacttaa
     1621 gccgcgcaga tcggtggcca gtaatgcgcc aacgacgcct aacggaacaa ccaacatcac
     1681 cgagaacgga attgaccagc tctcatagag tgcggcgagg gcgaggaaca ccacgaccag
     1741 tgaaatggca tacagtgcag gagcctggtt tgaggataac gcttcctgat acgacagtcc
     1801 ggtccatgag tagccgacgc ctgccggaag tttagcgacc aggtctgcca taaatttcat
     1861 agcgtcaccg gtacttttcc cggccgccgc ttcacctaaa atctccattg acgggatgcc
     1921 gttgtagcgt tccaatcgcg gtgaaccata ggtccattcg gtagacgagt aggcagaaag
     1981 cggtgccatc gtgccagagg cgttgcgtac ataccattgg ttgatgttat ccggcaacat
     2041 acggaacggc gtgcctgcct ggacatacac ttttttcacc cgcccctggt tgaggaagtc
     2101 gttcacgtag ctgctgccga aggcggtgga aattgtctgg ttgatatcag acagcgccac
     2161 acccattgcc tcagctttcg cggcgttgac gttcactttg aacatcggcg tatcttccag
     2221 gccgttcggg cgtaccccgg tgacctggtc aggcgattgc gctgccagtg ataacagctc
     2281 gtttcgtgcc tgggttagtt tttcgtgccc caggttaccg ttgtccagca gttccatatc
     2341 aaaacctgac gcggtaccca gttcagccac cgcgggtaag ttgaacggga agacgacggc
     2401 tttattgata ctgcttaacg caatcattgc ccgctgaatg atcgcggtaa ccgagttttc
     2461 ctcaccgaca cgttcagacc acggcttgag actgataaac gccaggccgt tgttttgccc
     2521 ctggccgctg aagccaaagc cgccaacggt aaacaccgac tgaacattat ctttctcttt
     2581 tgtaagataa taatccgtca cctgttgcag cactttcgtg gtgttaacca tggtggcacc
     2641 ggaaggtaac tgcgcggtgg tcataaatac cccctgatcc tcttctggta agaaagaggt
     2701 cggtgtacgc aggaacagca ccgccatccc ggcgcaaatc agcaggtaga ccaccatgta
     2761 gcgaccggta caacgcaaca gcgagcgggt gctatcggta tagtgctgag ttgatttttc
     2821 aaacagcgtg ttgaagcgtg cgaacagggc gttaggtttg tgaccgcctt ccggcgcggc
     2881 tttcagaatg gtggcgcaca gggcaggggt caggctcatt gccacaaata ctgaaagcag
     2941 catggaggag atcagcgtga tggagaactg gcggtagatc tccccggttg cgccgctcat
     3001 aaaggccatc ggcataaaca ctgcggaaag aacaacagca ataccgacca gcgcacgttg
     3061 gatctgcccc atcgatttat gcgtcgcttc cttcggtggt agcttatctt ccgcaatgac
     3121 acgctcgacg ttctccacca ccacgatggc gtcatccacc agtaacccta tcgccagcac
     3181 catcccgaac atagttaacg tgttgatggt gaaaccgacc gccgacaaga tcgcaaacgt
     3241 cccgagaata accaccggta cggcaatcgt cgggatgatt gtggcacgga aattctgcaa
     3301 aaacagatac atgaccagga agacgaggat gatagcctca accagtgttt taaaaacttc
     3361 ctgaatagaa atttcgataa acggcgtggt gtcgtaagga taaaccgtct tcagacttgc
     3421 ggggaaatag gctgataagc ggttcagctc ctctttgact gcccgcgagg tatccagggc
     3481 gtttgctccg gtagccagtt tgatggcgat cccggcagca ggtttgccgt tatagcgcgc
     3541 cacggtggaa taatcttccg ctccaagttc gacgcgggcg acatcacgca gcagcacttg
     3601 cgaaccatct tgctgaactt tcaacaggat tttgccaaat tcttccggcg tttgcagacg
     3661 cgtctgcaca atgatcgagg cgtttagttg ctggtctgcc gcctgtggca tgccacccag
     3721 ttgaccaccg gaaatctggt tgttttgcac cttaatctgg gaaataacat cggaaggtac
     3781 cagattgtat ttattgagtt tttgcgggtc cagccagata cgcatggcgt actcggaacc
     3841 aaagagctgt acgctaccaa cgcccgcggt acggcttagc gggtctttga tattagacgc
     3901 tacatagtcc gcgatatcgt actggttgag gctgccgtta tcagaaataa acgccgctac
     3961 catcagaata ttactgctcg acttatcaac gctaatcccc tgctgctgca ccgcttcagg
     4021 taatgaaggc atagcgagtt gcagtttatt ttgcacctga acctgtgcga tatcaggaga
     4081 tgtcccagtc tcgaaggtca gagtgataga ggcattaccc gccgcatcac tggttgaaga
     4141 catgtacatc aggccatcaa gcccattcat attttgctca atcacctgag tgaccgagtc
     4201 ttctaccgtt tgcgcatcgg cacctggata ggtagcgctg acggtaatgg tcggtggcgc
     4261 aatctgcgga tactgcgcaa ccggtaagtt catgatcgcc agaccacctg caagcatcat
     4321 aataatggca agtacccagg caaaaaccgg gcgatcaata aaatagttag ccatgaaagt
     4381 ccccttaagc ctgcaacgtt attgtttcga ttcggtgctg gcgttttcct ggctggagga
     4441 aattgctcgt gctttgatac ccggacgaat gcgttgcaaa ccggaaacga tcacccgatc
     4501 gccagcctgc aagccagagg tgacaaccca ctggtcgccg atggctttgc tggcttcaat
     4561 ttcgcgtagc tgcacaacat cgtctttatc cagaatgagc gccgttgctt taccctgggc
     4621 gttgtgagtg acgccttcct gcggcaccag taatacattc tggcggctac cttcatccac
     4681 taatgccgtg acgtacatgc caggcagcaa gtcaccattt gggttgggga aaatcgcccg
     4741 taacgtcacg gagcccgtgg tttcatccac cgtcgggtcg gagaatttca gtgtgccggt
     4801 ctggctgtag cgtttaccat tttccagatt gagctgtact ggcgtactgc cctgaacctg
     4861 tttgatttgc ccactggcga cctcttcttt catgcgcaag aaatcctgca ccgactgcgt
     4921 gagatcgaca taaatcgggt ccagacgttg tacggtaacc agcgaatctg cctgattagc
     4981 ggtaacgagt gcgccgacgg tcaccgacga tttcccgctg acgcccgtaa tcggcgaggt
     5041 gacattcgcg tattgcagat tgatcgtcgc ctgttcaaca gccgctttgg cgacggtgac
     5101 attggcttct gcttcattca gctgggtgcg cgcggtgtcg taatcctgac ggctaacgta
     5161 gttggtcttc agcaacgatg cctggcggtt aaaggtgatg cgggcattgc tggcggtaga
     5221 gagcgctttc gccagcgagc ctttggcgga gtttagctcg gcctgtaaag gtgcaggatc
     5281 aatctgatac agcgaatcgc cctggttcac tttatcgcct tcgataaagt tgcgtttaat
     5341 gataataccg cccacctggg gacgtatctc ggcaacttca taaggaacgg ttctaccggg
     5401 caattcgctc aacacattga ccgaaccggg ggagagtgtg acgacaccga cctcaggcgt
     5461 catggcggcg gcgttttccg ccgatttgtc atcgcaggcg gtgagcatcg cgccgcagaa
     5521 taacaacggt attaacagct ttcttcttct gttcatttta gtccctgaaa attcttgaga
     5581 aataacggtt ttttaaattg cgcgcgagga gagatatgag ggatttacag accagcggca
     5641 tagtggggtt gtcagccagt aaggtgggat acaggcacag tgatcgacat ggtgaggtca
     5701 acgacaaaaa aaatgttgcc gttctgccaa cagttcctgc cagcattcgg gcaacgaagg
     5761 gttctactgg tggatacaca tacctgggga ataaagtcgt tttagcctct cctttagtaa
     5821 tcaccggggg taatgaaatc agcatctgca tccctcgtca tgccagccat caatttcagt
     5881 tgcttatgtc ctgactaaaa ataagatgtg atacccaggg tgacgatgtc gctcatacgt
     5941 cttagtttca gcttgtacat aatgttgaat ttctgaatct ttaatgtttg ttgcgaaata
     6001 ttgagcgtcg acgtgatatt gctttcattt ttatttttca gaatcagttg cataattttg
     6061 aactgattat gtgaaaaaca taattgtgaa ttcttatgg
//
LOCUS       DAZOYL010000229         6087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZOYL010000229 DAZOYL010000000
VERSION     DAZOYL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.230"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUOY_003203"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUOY_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR0683915.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUOY_003204"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUOY_003204"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR0683916.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="ACHUOY_003205"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="ACHUOY_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR0683917.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUOY_003206"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUOY_003206"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR0683918.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         1617 a         1609 c         1449 g         1412 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcgg
//
LOCUS       DAZOYL010000230         6069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZOYL010000230 DAZOYL010000000
VERSION     DAZOYL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.231"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(252..998)
                     /gene="cutC"
                     /locus_tag="ACHUOY_003207"
     CDS             complement(252..998)
                     /gene="cutC"
                     /locus_tag="ACHUOY_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR0683919.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(1012..1578)
                     /locus_tag="ACHUOY_003208"
     CDS             complement(1012..1578)
                     /locus_tag="ACHUOY_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0683920.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1794..3530
                     /gene="argS"
                     /locus_tag="ACHUOY_003209"
     CDS             1794..3530
                     /gene="argS"
                     /locus_tag="ACHUOY_003209"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR0683921.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(3572..3616)
                     /gene="yecV"
                     /locus_tag="ACHUOY_003210"
     CDS             complement(3572..3616)
                     /gene="yecV"
                     /locus_tag="ACHUOY_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR0683922.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3707..4069
                     /locus_tag="ACHUOY_003211"
                     /pseudo
     CDS             3707..4069
                     /locus_tag="ACHUOY_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(4073..5143)
                     /locus_tag="ACHUOY_003212"
                     /pseudo
     CDS             complement(4073..5143)
                     /locus_tag="ACHUOY_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(5136..>6069)
                     /gene="flhB"
                     /locus_tag="ACHUOY_003213"
     CDS             complement(5136..>6069)
                     /gene="flhB"
                     /locus_tag="ACHUOY_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR0683923.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1581 a         1603 c         1489 g         1396 t
ORIGIN      
        1 taattgctaa ggaataaccc agttgctatt tagaatatat atttatcagt tttagcaatt
       61 taaatcccat aatcaatgtg aatataaaaa atattattag cacaccgcta tgttcactgt
      121 cacccgctca acagatcacg gttatcgttc gttttttata ctgctcaggg ataaaaaaag
      181 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
      241 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
      301 gccccgtcta cgatataacg cgaatactcg tccgcgtgtt catctgatga catggacaat
      361 ccttgattac gataacgcat cggtgaggct tgccacgctc ccgcggagct atggacttcc
      421 agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc tccggccata
      481 atgattggag catcacgatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
      541 tctgattttt gccctgatgt cagtacccgg gcaatgccca attccgcaag attattgagt
      601 gtatataaag ggttagcgca catatcgaag gcgcgatgaa aagtcactgc cagcggaccg
      661 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac atcgagaacg
      721 cccgtcacca gtccaggaaa acctaattcg cggaccgtgc gcacatcctc aagaatggcg
      781 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaattat cggatgcaca
      841 gggatcgtca cccgctggcg cacggatttc agtaccccca acgacggcgt taagcccccc
      901 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca
      961 cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta attacgctga
     1021 ctgctcgctg gcaacgatct cttcaatgga ccacggatga aacttaatag ttgttttgcc
     1081 atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg gtgaactggt
     1141 tttaactgag atccccggta ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     1201 attcagcgtt tctggatcac ccagcagaac gatttcaata tgttcccaac cttcgtgtgg
     1261 gtaacgtttt tccccgggcc acggtagctc cacaatagaa aactgccagt gcgcaacctg
     1321 taccggttca tgcaatttaa acagacaaat cggtctgcca ttgatcatat tttctgacaa
     1381 aagctcgcca cactgttcaa acccgcgacg ccagcgttca gcagtggcgt tttgatggca
     1441 acgcaaagaa atgtgatcgg cagtcagcgg agtgatattc aaccccagac ggagggaaag
     1501 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcgtcgat
     1561 agattgccag ttcgccataa tcactcttcg tctttcagta aaagcgttaa tttaccctgt
     1621 tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacggca
     1681 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     1741 tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     1801 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     1861 attgcgaacc gcaggttcgt cattcagcaa aagttcagtt cggcgactat caggctaacg
     1921 gcatgatggc agttgctaaa aaactgggta tgacaccgcg acaattagca gagcaggtgc
     1981 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt ccaggcttta
     2041 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     2101 atcgtctcgg tgtttctacg ccagaaaaac agaccattgt ggttgactac tctgcgccaa
     2161 acgtggcgaa agagatgcat gtcggtcacc tgcgctctac cattattggt gacgcagcag
     2221 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
     2281 gcactcagtt cggtatgctg attgcatggc tggaaaagca gcagcaggaa aacgccggtg
     2341 atgaaatgga gctggctgac cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg
     2401 aagatgaaga gtttgccgag cgtgcacgta actacgtggt aaaactgcaa agcggtgacg
     2461 aatatttccg cgagatgtgg cgcaaactgg tcgacatcac catgacgcag aaccagatca
     2521 cctacgatcg tctcaacgtg acgctgaccc gtgatgacgt gatgggcgaa agcctctaca
     2581 acccaatgct gccaggaatt gtggcggatc tcaaagccaa aggtctggca gtagaaagcg
     2641 aaggggcgac cgtcgtattc cttgatgagt ttaaaaacaa ggaaggcgaa ccgatgggcg
     2701 tgatcattca gaagaaagat ggcggctatc tctacaccac cactgatatc gcctgtgcga
     2761 aatatcgtta tgaaacactg catgccgatc gcgtgctgta ttacatcgac tcccgtcagc
     2821 atcaacacct gatgcaggca tgggcgatcg tccgtaaagc aggctatgta ccggaatccg
     2881 taccgctgga acaccacatg ttcggcatga tgctgggtaa agacggcaaa ccgttcaaaa
     2941 cccgcgcggg tggtacagtg aaactggccg atctgctgga tgaagccctg gaacgtgcac
     3001 gccgtctggt ggcagaaaag aacccggata tgccagctga cgagctggaa aaactggcta
     3061 acgcggttgg tattggtgcg gtgaaatatg cggatctctc caaaaaccgc accacggact
     3121 acatcttcga ctgggacaac atgctggcgt ttgagggtaa taccgcgcca tacatgcagt
     3181 atgcttacac gcgtgtattg tccgtgttcc gtaaggcaga aattaacgaa gagcaactgg
     3241 ctgcagctcc ggtaatcatc cgtgaagatc gtgaagcgca actggcagct cgcctgctgc
     3301 agtttgaaga aaccctcacc gtggttgccc gtgaaggcac gccgcatgtg atgtgtgctt
     3361 acctgtacga tctggctggt ctgttctctg gcttctacga gcactgcccg atcctcagcg
     3421 cagaaaacga agaagtgcgt aacagccgtc tgaaactggc acaactgaca gcgaagacgc
     3481 taaagctggg tctggatacg ctgggtattg agacagtaga gcgtatgtaa tcgatttttc
     3541 gtgagagtaa agcctgatca gggttacctg atcaggcttt tttattgcca tctaaatgta
     3601 ttctgaaaat ggacatgcca ttgttttctg actgttggat aagaggccag aagcgtaata
     3661 tccggcccca gggaaacgat aacggttgaa tttaaggaat accgcagtgt ttaaatttct
     3721 tgtattaaca ttaggcatta tctcttgcca ggcttacgca gaagatacgg ttatagaaaa
     3781 cgaccatgac atttcagcca tcaaagattg ttggcaaaaa aattcagatg atgatactga
     3841 cattaacgtg atcaaatcat gcctgcgaca agaatacaat ctcgtcgatg cgcaattaaa
     3901 taaagcctat ggtgaagctt atcgttatat agaacaagtg ccacgcacag gtgtaaaaaa
     3961 acctgatacc gaacaactta acttgcttaa aaaatcacag cgagcctggc tggattttag
     4021 ggacaaagaa tgtgaattaa tcctttcaaa tgaggacgtt caggatttaa tgtacatcgt
     4081 tccacgtcgc ttcgacaacg gtattattct ctgccatttt taccggtttg ggttcggcag
     4141 gcgctttttg ttcgcgtccg cgtatccacc aggccagccc gagcaatccg gcagtgaaca
     4201 gcaaaaatac caggttcggc attccaggca ccaggccgag taaaccgagc acggcggcgc
     4261 ttaacaacat aacgcttggg ttactgaaaa gctgattcac catctgctcg ccaacatcct
     4321 gatcggtgct gacacgcgta acgatgaccc ccgcggcggt aaaaatcacc agcgccggaa
     4381 tttgtgccac cagaccatcg ccaatggtca atagcgtata actttccgcc gcgtgtccca
     4441 tgctcatgcc atgttgcagc acgccgacca gcaacccgcc gacaacgtta atgaccatga
     4501 tgaggatccc ggcgatggca tcgccgcgaa caaacttact tgccccgtcc attgagccgt
     4561 aaaaatcggc ttcctgagtc acttcggagc ggcgtttttt cgcctcatct tcaccaatca
     4621 atccagcgtt aaggtcggcg tcaatcgcca tctgcttacc cggcatacca tcgagaacaa
     4681 agcgcgcacc cacttctgcg atacgcccgg cacctttggt aatgaccata aagttgatga
     4741 tcacgagaat gacaaacacc acgataccga tagcgaaatt gccaccaacg aggaagtgac
     4801 cgaacgcttc gaccaccttc cctgccgccg ccgcgccggt atgcccttcc atcaaaatga
     4861 tacgggttga agccacgtta agtgccagac gcaacaacgt agtaaacaac agaatggtcg
     4921 gaaacgcagc aaactcaagc gtgcgctggg taaacatcgc caccagcaac accatgatcg
     4981 acaaggcaat attgaaggta aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca
     5041 tcatcgacaa gatcaacagg atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt
     5101 tcaggtttgc gggcaggcgc agcatcgcgg ccagattact catgggtcgg tttctcgtta
     5161 ataaaatcca gggcttccgg caccggaaga tgagtaggtt gtacagggcg ctgtccacca
     5221 gccagacgcc agcgtttcag ttgccagacc caggccagca cttccgccac cgcggcgtac
     5281 agttgacccg ggatttgttg accaatctcc gcatgtcgat acagcgctcg cgccagcggc
     5341 ggcgcttcaa gcgtcgggac gttattttca gcgccaattt cacgaatgcg cagcgcgacc
     5401 agccctgcac ctttagcgac cactttcggt gcgctcattt tgttttcgtc atactgcaac
     5461 gctaccgaat agtgggtcgg gttattgaca atgacatcag ctttcggcac atcggccatc
     5521 atccgacgcc gtgcagcagc tcgctgcatc tgacggatcc gccctttaac atgagggtca
     5581 ccttcgcttt gtttgaactc atcacgaata tcctgccgcg acatacgcag ctttttcagg
     5641 tggctgaaga tttggaaaaa gacgtcaaat cccaccattg gaatgacacc aagcaccacc
     5701 agcagtgcgc atagcccaac cagatccatc gcattaccca tggcggtagt cggagactcg
     5761 gccattaagc gcatcatctg cggccagtga tgccagagat aaaaccccgt cacgctgcca
     5821 accaggatgg ttttcaaaat tgccttaagc aactccgcgc cagtctgtgc cgagaacatc
     5881 cgtttaatgc ccggtagcgg gttgagtttg gaaaacttcg gctgcaagga tttgccgcta
     5941 aataccagcc cccccagcat gaccggagaa atgagcgcca ccagcaccac gccgctaatc
     6001 agcggcagca gcgccagcat ggcttctctg atcagcagaa taatctgccc gaggatcaga
     6061 ttcgggtca
//
LOCUS       DAZOYL010000231         6015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZOYL010000231 DAZOYL010000000
VERSION     DAZOYL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.232"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(369..1304)
                     /locus_tag="ACHUOY_003214"
     CDS             complement(369..1304)
                     /locus_tag="ACHUOY_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0683924.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     TSARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
     gene            complement(1802..1945)
                     /locus_tag="ACHUOY_003215"
     CDS             complement(1802..1945)
                     /locus_tag="ACHUOY_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFR0683925.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGV
                     ETQ"
     gene            complement(2029..2286)
                     /locus_tag="ACHUOY_003216"
     CDS             complement(2029..2286)
                     /locus_tag="ACHUOY_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="HFR0683926.1"
                     /translation="MGLKLHIHWFDKKNEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVEKRWVPILQPYFKNVIDISKFDYFVSFDYRDGDW"
     gene            complement(2296..3933)
                     /locus_tag="ACHUOY_003217"
     CDS             complement(2296..3933)
                     /locus_tag="ACHUOY_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HFR0683927.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVS
                     GEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTN
                     VQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASF
                     PGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKP
                     VYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQ
                     RAQTDVNNKKAAFDAAAKEKSAADAALSSAMESRKKKEDKKRSAENKLNEEKNKRRKG
                     TKDYGHDYHPAPKTEDIKGLGELKEGRPKTPKQGGGGKRARWYGDKGRKIYEWDSQHG
                     ELEGYRASDGQHLGSFDPKTGKQLKGPDPKRSIKKYL"
     gene            complement(4154..4687)
                     /locus_tag="ACHUOY_003218"
     CDS             complement(4154..4687)
                     /locus_tag="ACHUOY_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683928.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(4690..5616)
                     /locus_tag="ACHUOY_003219"
     CDS             complement(4690..5616)
                     /locus_tag="ACHUOY_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR0683929.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
     gene            5792..>6015
                     /locus_tag="ACHUOY_003220"
     CDS             5792..>6015
                     /locus_tag="ACHUOY_003220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0683930.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEG"
BASE COUNT         1487 a         1660 c         1297 g         1571 t
ORIGIN      
        1 aaaaaaacaa cgacgagaac gcaatcaccg actctttctc gcggctgggc tactgatcga
       61 agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca
      121 ggcacttaag gaaattaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc
      181 tccatccgtg ttcctgttcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat
      241 gagactgcgg cgagcactac cagcacaatc atgcggaaaa cagcaccatt gcagggctga
      301 acggggaaaa acggcaaaag acctaaggct caaaaaaacg ccagaaaggc gctgttatct
      361 gatataggct attttccgct tttccagttt cgaatggttc gagtagaaac ctccaacatc
      421 gccgcaatag ctttctgaga atatccattc tccagcatac atagcgccat gaaacgcttg
      481 tcatcgtagg cttcgccttt agcgatgcca cctttcctgc ctctcttggc ctgtatttct
      541 ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg ccgtgaagtt cctgtgcgtg
      601 tatttcgcaa tactcttgcc aatagcccga cattcaggag gtgaaagcgg aacgggaagc
      661 gatgcgttgt acatttcgac acgctgaatc acggcatcaa gccactgtga gaatgctggc
      721 cagccctgac gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag atggcagttt
      781 ctccccattc cgtaatgttt atcgatgcta cggcgcgctg aagtgctcaa atcgagataa
      841 tcagccagtt catcgagggt ataggcttcc tcacgccact ccatcacctg ccattccaga
      901 tggaacggat ttttgcaaat cagtccgctg tagtttacat ctgcgcccag tttttcacac
      961 aacgcacgtt caatcgcggc ggcgtatttc agcgccttaa ccgaagcatc aggcgcggtt
     1021 ctcacggcga tattgagtgc gtagagcagg tgggcgtgac cgttcacggg attttttatg
     1081 gtgatgttcg gtgcgggggc gttcaggtcg ctccagtcaa tcgcagcccc ctcgcggtca
     1141 acgtcaaaaa ccagccagaa ctgcgcatga ggttggttct gctggatata ccgtgcaaga
     1201 agcgcgcgcc ctttaccgga gatacgaagg ccgaaagcga gatcatccgt gtgatagggg
     1261 cggaggggta aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc
     1321 caggttatcg accgacctcg cccctgtatt ctcgtgacaa tcttggcgga gagacacgaa
     1381 tttcaggggc gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgatc
     1441 cttcccgaaa acccgatcca tttaccataa ctcccgttgt acgtacccct taaaacgccc
     1501 tcaatcgctt tctggcgcaa aaaacgcaaa aagacaagaa aacaccagga caaaccagaa
     1561 aaacgcgtca gaatcgtttt cagggcgttg cataactatg cacgaaagcg atgtgcgatc
     1621 ggtttcgtgc gttctgaaca ccgaacggct caaaacgaaa agttttctcc ctccggatgg
     1681 aaggttctgc acggagattt tagatcccat ctcccacatg gaatttttgt ggcacttcaa
     1741 caaaaaaagc gcgtcagaat cagcctgaca cgctttgtcc ttgtcaaatg aggattgcag
     1801 gttactgcgt ttccactccg gtcagttcag cagaggatga cggtgataca gtcccgccct
     1861 gaacatcacg gatatagttt gcctgacatg cagcaagaat aatggctgca agaagcaaca
     1921 aaataatccc tgttattttc ttcatacgat tccctgtata tatggattca ggaattcatc
     1981 cttgaacacc tgaatcaaac actttattac tcgtccctga tagtttaatt accagtcacc
     2041 atcacgataa tcgaaagaaa caaagtaatc gaacttacta atatcaataa catttttaaa
     2101 gtatggctgt aatattggca cccatctctt ttcaacatca aaccaaccgt tattaatatt
     2161 atcctttaaa ggcattccaa gacgttcaat aaccgaacca tcatcaccga aatcctttga
     2221 atactcacca cctttaaatt cctcattctt cttatcaaac caatgaatgt gtaatttaag
     2281 tcccataaca tcaccttaaa gatacttttt aatacttcgt ttcggatctg gacctttcaa
     2341 ctgcttaccg gttttagggt caaatgaacc aagatgctga ccatcactgg ctcgatagcc
     2401 ctcaagctca ccatgctgag agtcccactc ataaatttta cgccccttat ctccatacca
     2461 tctggcacgc ttacctccac caccttgctt tggagttttt ggcctcccct cttttaattc
     2521 ccccaaacct ttaatatcct cagttttagg agctggatga taatcatggc cataatcttt
     2581 agtgcctttt cggcgtttat ttttctcttc gtttaattta ttttcagcac tccttttctt
     2641 atcttctttc ttcttcctgc tttccatcgc agaactcaat gcagcatctg ctgctgactt
     2701 ctcttttgcg gcagcatcaa atgcagcctt cttattattt acatccgtct gtgcccgctg
     2761 ggccttaagc cctgccattt gccacattct gtgaccagca gccattggtt ctcgggcaaa
     2821 tcgctcgaat tgttttattt cagcctttgc atcagcaaga gttttattcg ctgcatcaag
     2881 ttcactttta cgagaattat aaacctgaac agctttagcc tgtcgttcct ggtttctggc
     2941 aacatcttta ttagcctgat tcagttctgc gcgggcctgt tcataattgc gttcagccac
     3001 ttccaccgga tgtgcgtcat tccattcctg ctggaggcgt ttttcctcat cctgacgttg
     3061 ttttacctgt gctggggtaa gaacatctgt cactgataca taaaccggct tctgcccact
     3121 ttctttcgga aagcgaatca ccgcttcatg tgaaccacca ccaaaggtaa atcccgcagg
     3181 aacggcagtc cggcctttat cctccgtaat accacgggga agggttgttg acaccggaag
     3241 gcctttgaca gtgctgactg tcagagaagg gacaccagga aatgatgcgt gaaatacacc
     3301 tggagtacgt gttggtttgg cattcacaac aggcacactc ataggcacac ctgcaactac
     3361 ggcaatatgc tgtcgtgtgt ccttcacaac atctgtcaca cgtttcgtca cgctgacagt
     3421 tgcctggtcc agcggaagtg tactgacctg aacattcgtc actgtttctg ccggtaatga
     3481 cgtcacaatc tttgacatca tattcgggtc atcttttgct atttcagatg gcagaatgcc
     3541 gtaaagcgca ataccccatg cgctgaattt aaacgggcct ttcagggctg caaagatatc
     3601 cgctattgcc gcagataaag cctcaccaga gacggcgata cccagcgttc cggcaccagg
     3661 agttgccaaa gcaggaaaac caaatgccac tgctgctacg gatgaattgc tgccaccacc
     3721 ggaattacca ttccccccgc cattaccatt gccggagcca cctccccagt gaacaccact
     3781 accggaaccg ccaccccatg ggttattttc cgaactccat cctgaaccgt cagaagcccc
     3841 accatttcca ccaagccccg tagggccgcc attaatgtta ccacctgtgt tatgtgcgcc
     3901 actgttgtgg tcgcgtccat ctccaccgct cataaaaatt ccctctttta agcgttaaaa
     3961 caattgatta tatagttgat actgtacata aaaccactgg ttttatgtac agaccaaaaa
     4021 gtactcgcca ttattttttc tgtcaagtca gggggggata ttttttggca gttcggagga
     4081 atcactcatg aaaacggtga acggaacgcg aaatcctttc gcgggtcgtg aggcgttgga
     4141 caagcgaagc gcgtcagttt ttccggctct tacccgtgac aggtttctcc accagtttat
     4201 cttccagtgt cacaactttc tgatacaccg ccgctgtggc gttgtagttc ctgtcgagaa
     4261 ttgttttcat cccccatccg agcacactga acataaccag aaaggccact gacaccccac
     4321 ccagccattt ccagctatac cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa
     4381 ttgtcgcaat atccctgttc agggcatccg cgtttgcctt catgtttgcg actgactctt
     4441 ccagccgctg aaaatcctga ctgatgcgct cacctatcag ccctgcaaca tccagcctct
     4501 gcacttcgtt tctgacttca tccctgacct gttttgccac accgtcagaa atatcccgat
     4561 atccgcgaag tgtttccctt gccacagcca gagaatcagc aacagctttc tgttgctcca
     4621 ttgcggtatg ttgcagatgc tccaccgata acaggagcgt gagtaatttt tcttctgtgc
     4681 tggccatgtt cagaatccca tagaaaatcc acgtgacggt gatttaaccc gttcaggttc
     4741 agttcggttc tgtctgctca ctctgtctct catgtcaaag ccatgctccc gcgcaacatc
     4801 atgttgcagc cttctgacca gttcatgttg cttaaccgga gtgtccagcc attccctgat
     4861 gtcatgcccg accgttcgcg cctgttcaag atggcgggaa tagtccggtt cagaccgttt
     4921 ttgcatgatg tcatgacgct gggcatcatc aagccgtccg gcagcttcaa aacccagatg
     4981 cacttcggga cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct
     5041 ttcaaagcca tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc
     5101 ccgcaactgc tctttttgct gagtgagagt caggcttccg gaatcctttt tcgcacctcc
     5161 ccgctcggga tacctggcgt tatagcgccg gaaatactgt tccggtgtac gttcaatgcc
     5221 atcattcaca cgctggctca tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct
     5281 cgctttcgga ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt cctgacgtac
     5341 aaaatcctgc accagttcaa ggcgctgttc cgctgtcagt tcacggggta atgcaatttc
     5401 aagttcccga taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg
     5461 tgagggatta tctcgcgccc attccggcat gtttccgtgt gcggaatatt ccaaatcctg
     5521 acggtcgcgg tatttctctt tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt
     5581 gccaccaaat ttaacgctga ggtgataggt tgccacggtt tcagccctcc gcaggagcgc
     5641 actgtttctc gacgaaggag agaaacatat aagtgcgctt ccttctaatt ttacgaatta
     5701 gcacggaaga taccccctgc acccaccgac cgcaagcgat atactgcacg accacaacac
     5761 acagcaaaaa acaccaggga gcaccacgaa catgcgcatc agcaacatcg aatggctgaa
     5821 gaaacggatc ggattcatca ggaagctcgg agaacagacc gcacgacaga gacagattat
     5881 tgacctgctc gacaacgaag caggactcac agaacaggaa cgcaaactcc tccacgtact
     5941 ggcaacagcg gaaaaaaacg acttacaggc acaggagagc gaacgaaaac aggcggtcca
     6001 aaaacgaatc gaggg
//
LOCUS       DAZOYL010000232         6015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZOYL010000232 DAZOYL010000000
VERSION     DAZOYL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.233"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHUOY_003221"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHUOY_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0683931.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUOY_003222"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUOY_003222"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR0683932.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHUOY_003223"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHUOY_003223"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR0683933.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUOY_003224"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUOY_003224"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR0683934.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUOY_003225"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUOY_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR0683935.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1427 a         1517 c         1634 g         1437 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataa
//
LOCUS       DAZOYL010000233         5997 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZOYL010000233 DAZOYL010000000
VERSION     DAZOYL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.234"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(44..1330)
                     /gene="thrC"
                     /locus_tag="ACHUOY_003226"
     CDS             complement(44..1330)
                     /gene="thrC"
                     /locus_tag="ACHUOY_003226"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR0683936.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1331..2263)
                     /gene="thrB"
                     /locus_tag="ACHUOY_003227"
     CDS             complement(1331..2263)
                     /gene="thrB"
                     /locus_tag="ACHUOY_003227"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR0683937.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2265..4727)
                     /gene="thrA"
                     /locus_tag="ACHUOY_003228"
     CDS             complement(2265..4727)
                     /gene="thrA"
                     /locus_tag="ACHUOY_003228"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR0683938.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(4754..4873)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(4809..4874)
                     /gene="thrL"
                     /locus_tag="ACHUOY_003229"
     CDS             complement(4809..4874)
                     /gene="thrL"
                     /locus_tag="ACHUOY_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR0683939.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5088..5774)
                     /locus_tag="ACHUOY_003230"
     CDS             complement(5088..5774)
                     /locus_tag="ACHUOY_003230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0683940.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         1457 a         1663 c         1449 g         1428 t
ORIGIN      
        1 tgcataaaag caaacccggc ctgattgaga taatgaatag attttactga tgattcatca
       61 tcaatttacg caacgcagca aaatcggcgg gcagattatg tgaaagcaag ggtaaatcag
      121 cacgttctgc cagctctttt ggcagatcca acgtttcacc gagaatcgct tccacgctct
      181 ctttaaattt cgccggatgc gcggtgccga ggaacaagcc atattcgcct ggattcaact
      241 ggtcacacag cgcacgatac gctacggcag cgtgcggctc cgaggtgtag cccagttctt
      301 ttaactcacg cattgtctgt tgcgtggttt catcatccac ggctgcataa cccagctctt
      361 tcagttgcca gattttgcgg cggaacaact cttccacacg cggccagttg ttcggctgac
      421 tcacgtccat cgcgttggat aacgtcgcct gagtcgcttt gggtgaccac tgaccgtcgt
      481 gcaggaaacg tggcacggta tcgttcacgt tggtcgcagc aataaaacgt ttcaccggca
      541 gaccgagtga cttcgccagc agacccgccg tcaaatcgcc gaagtttccg cttggcaccg
      601 agacaaccag ctggttgcgc gcttcctgcg gcagctgcgc aactgcttca aagtagtagc
      661 aaatctgcgc cagcaaacgg ctgatgttaa tcgagttagc cgagtttaac cccagcgcca
      721 ctttcagttc ttcatcatca aacgcctgct tcaccagcgc ctgacaggca tcgaaatcgc
      781 cgtcgatggc aacagtttcg atattgccgc ccaacgtaca gaacagtttt tcttgcagtg
      841 gactgatttt gcctcgtgga tagaggataa ccactttcac attcggcaaa ccgtagaaag
      901 catgagccac tgccgctccg gtatcaccgg aggtcgcggt cagaatggtc actggcttat
      961 cgcccgcaat atgggtcagc atttgtgcca taaagcgacc gccgaaatct ttaaatgcca
     1021 gcgttggccc gtggaacaat tccagacaac cgacatcgct ttcaacattg gcgaccggag
     1081 ccgggaaggc aaacgccgcg cgcacgcgct cttccaggat ttcctgcggg atttcatcac
     1141 caataaacgc cgagaggatc ttcgcactgc gggtgacaaa atccagcttc agcatctcat
     1201 caatttcagt caggctgaat tccggcaggt catgcggaaa aaacagcccc tgatttttgc
     1261 ccaacccctg ggttacggct tgcgcaaagc tgacctgctc gttgtgatct ttcagattgt
     1321 agagtttcat ttagttttcc agtactcgtg cgcccgccgt atccagccgg caaatatgaa
     1381 caaaaccttc ctgattttgc aggtagttct tacccaacca gtcggcaacg cgctgggcgg
     1441 tatccggctt gtcacataga gcgaacaagg tcgggccgga gccggagata ccgctcgcta
     1501 ccgcgccgat ttccgcgacc gcctgccgcg cctgccggaa gccaggcagt aaccgttcac
     1561 ggtagggttc agcgataaca tctttcatca gcttcgcggc aagctcaggc tgacgggaat
     1621 agcaggcgtg aatgaagcca gccagatgtc gcccgtgcgc aatgcaatcc tggcggcgat
     1681 actgcgccgg taaaatagcc cgggcttctg ccgtcgagac tttaattccc ggatacgcca
     1741 gcacccacag ccactcatca aaccctggca cttgctggct gatgatgtcg ttttcttcga
     1801 tcatcaactg cataccacca agaaaacacg gtgccacgtt gtcgtaatga atgctgccgg
     1861 agatacgtcc ttccagctcg cccatcaaag ccagcaaacg ggtgtcatta agtggcttgc
     1921 cgcagtgttc attcatcgcc atcagcgccg cgaccaccga acaggcgctg gagcctaagc
     1981 ccgagccgat cggcatattc ttttccagag tcatcgccac tggaatttgc ttgcccagct
     2041 ccaggcaaaa acgctcccag cactgataaa cgatattttc ccgtggttct gacggcagct
     2101 tatcggcaaa gcgtccgagg ttgttgagac tgaatgtctc tgccgcctca accgtgacta
     2161 catctccgag caatgcacca tcaacgggtg tcaccgccgc cccgagcaca tcaaacccga
     2221 cgctcatatt ggcactggaa gccggggcat aaactttaac catgtcagac tcctaacttc
     2281 catgagaggg tacgtagcag atcggcaaag acaccagcag cggtaacgtc attgcccgca
     2341 ccatatccgc gcagcaccaa cggcagcggc tgataatagt ggctataaaa ggccagggcg
     2401 ttttcgccat ttttcacttt gaacagcgga tcattcccat ccacttcggc aatcttcacg
     2461 cggcagacac catcttcatc aatattgcca acatagcgca aaacttttcc ttcatcacgg
     2521 gccttcgcca cgcgcgcggc aaagagatcg tcgagctgtg acagattcgc cataaaagcg
     2581 gcaacatcac cctcggcgtt aaactctgcg ggcagcacag gttcaatttc aatatccgcc
     2641 agttccagtt cacgtcccgt ttcacgagcg agaatcaata gtttacgcgc cacatccata
     2701 ccagaaagat catctcgcgg gtccggttcg gtataaccca tttcccgcgc cagcgtggtc
     2761 gcctcggaga aactcatgcc ttcgtctaac ttgccgaaga tataagaaag cgaacctgaa
     2821 agaatgccgg agaacttcac caattcatca cctgcattga gcagattttg caggttctca
     2881 ataaccggta atccagcccc aacgttggtg tcatagagga atttacgccg cgatttttcc
     2941 gccgcataac gcaactgatg atagtaatcc atcgacgagg tgttggcctt tttgttcggc
     3001 gtgacaacgt ggaaaccttc gcgcaggaag tcggcatatt gatccgccac tgcctggctg
     3061 gaggtgcagt caacaatgac cgggttcagc agatgatatt ctttcacgag gcgaattaag
     3121 cgcccgagat taaacggctc tttggcttgt gcaagttctt cctgccagtt ttccagatta
     3181 aggccatgca cactagtgag cagagccttc gagttggcaa cgccgcagac acgtaagtcg
     3241 atatgtttat tcttcagcca gctttgctga cgcttcagtt gctccagcag cgcaccgcca
     3301 acgccaccga cgccaatcac aaacacttcg ataacctgat cggtattgaa cagcatctga
     3361 tgagtaacgc gcacgccagt ggtcgcatca tcgttattta ccacgacaga gattgagcgt
     3421 tcagaagatc cctgagcaat ggcgacaatg ttgatattgg cgcgggccag cgcggcaaag
     3481 aatttcgccg agatcccacg caaggtgcgc ataccatcac ctaccaccga gataatggcc
     3541 agccgttcca tcaccgccag cggctccagt aagccttctt tcagttccag gtagaactct
     3601 tcctgcattg cccgttcagc ccgcacacag tcgctttgcg gaacgcagaa actgatactg
     3661 tattcggaag atgattgcgt aatcagcacc acggaaatac gggcgcgtga catcgcggca
     3721 aagacgcgcg ccgccatgcc gaccattcct ttcatccccg ggccggaaac gctgaacatt
     3781 gccatgttat tcagattgga aatgcccttg accggtaatt cgccttcatc acgactggca
     3841 ccaatgagcg tacctggtgc ttgaggattt ccggtatttt taatcaggca agggatctgg
     3901 aactgggcga tgggggtaat ggtgcggggg tgaagaactt tagcgccgaa gtaggaaagc
     3961 tccatcgctt cctggtagga catcgacttc aacaacctcg catcgggcac ctgacgcggg
     4021 tcgcaggtat agaccccgtc aacgtccgtc caaatctcgc aacaatcggc gcgtaaacag
     4081 gcagccagca ccgccgcgga gtagtcggaa ccgttgcgtc caagtaccac cagttcgcct
     4141 ttttcattac cggcggtgaa gcctgccatc agcaccatgt gatcagccgg aatacgactt
     4201 gccgcaatac ggcgggtgga ctcagcaata tcgacggtag attcgaggta atgccccact
     4261 gccagtagtt tttcgaccgg atcgataacg gtaacgttgt gaccacgcgc ttctaacacg
     4321 ccggccataa tggcgatcga cattttctcg ccacggcaaa tcagcgcagc gttaatgcta
     4381 tccgggcact gccctaacaa actaatgcca tgcaggacat gttttatttg ggcaaattcc
     4441 tggtcgacga aagttttcaa ttgcgccagc gggaatcccg gctgggcggc ggcgagtccc
     4501 gtcaaaagtt cggcaaaaat acgttcggca tcgctgatat tgggtaaagc atcctggccg
     4561 ctaatggttt tttcaatcat cgccaccagg tggttggtga ttttggcggg ggcagagagg
     4621 acggtggcca cctgcccctg cctggcattg ctttccagaa tatcggcaac ccgcagaaaa
     4681 cgttctgcat ttgccactga tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg
     4741 ttacctttgg tcgaaaaaaa aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc
     4801 tgtacgcgtc agcccgcacc gttacctgtg gtaatggtga tggtggtggt aatggtggtg
     4861 ctaatgcgtt tcatggatgt tgtgtactct gtaattttta tctgtctgtg cgctatgcct
     4921 atattggtta aagtatttag tgacctaagt caataaaatt ttaatttact catggcaggt
     4981 aaccagttca gaagctgcta tcagacactc tttttttaat ccacacagag acatattgcc
     5041 cgttgcagtc agaatgaaaa gctgaaaaat acttactaag gcgtttttta tttggtgata
     5101 tttttttcaa tatcatgcag caaacggtgc aacattgccg tgtctcgttg ctctaaaagc
     5161 cccaggcgtt gctgtaacca gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc
     5221 agcaatgcca tgactcgttc gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca
     5281 cttttcgccg gttgttgtat taatgttgct aattgatagc aatagaccat caccgcctgc
     5341 cccagattga gcgaaggata atccgccacc atcggcacac cagtaagaac gtcagccaac
     5401 gctaactctt cgttagtcag cccggaatct tcgcgaccaa acaccagcgc ggcatggctc
     5461 atccatgaag atttttcctc taacagcggc accagttcta ctggcgtggc gtagtaatga
     5521 tatttcgccc gactgcgcgc agtggtggcg acagtgaaat cgacatcgtg taacgattca
     5581 gccagtgttg ggaaaacttt aatattatca ataacatcac cagatccatg tgcgacccag
     5641 cgggtggctg gctccaggtg tgcctgacta tcgacaatcc gcagttcgct aaaacccatc
     5701 gttttcattg cccgcgccgc tgccccaata ttttctgctc tggcgggtgc gaccaggata
     5761 atcgttatac gcatattgcc actcttcttg accaaataac cgcgaacagg gtgatcactg
     5821 tcaacttatt acgcggtgcg aatttacaaa ttcttaacgt aagtcgcaga aaaagccctt
     5881 tacttagctt aaaaaaggct aaactatttc ctgactgtac taacggttta attgttaaaa
     5941 aatgctacat atccttctgt ttacttagga taattttata aaaaataaat ctcgaca
//
LOCUS       DAZOYL010000234         5989 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZOYL010000234 DAZOYL010000000
VERSION     DAZOYL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.235"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHUOY_003231"
     tRNA            292..368
                     /locus_tag="ACHUOY_003231"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUOY_003232"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUOY_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR0683941.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHUOY_003233"
     CDS             complement(1357..2247)
                     /locus_tag="ACHUOY_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR0683942.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUOY_003234"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUOY_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR0683943.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..5947)
                     /gene="nfrB"
                     /locus_tag="ACHUOY_003235"
                     /pseudo
     CDS             complement(5207..5947)
                     /gene="nfrB"
                     /locus_tag="ACHUOY_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1360 a         1568 c         1563 g         1498 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttcgg
     5941 taatgactcc aacttattga tggtgtttta tgttcagata atgcccgat
//
LOCUS       DAZOYL010000235         5941 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZOYL010000235 DAZOYL010000000
VERSION     DAZOYL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.236"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(64..1314)
                     /gene="icd"
                     /locus_tag="ACHUOY_003236"
     CDS             complement(64..1314)
                     /gene="icd"
                     /locus_tag="ACHUOY_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR0683944.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1486..2139
                     /gene="rluE"
                     /locus_tag="ACHUOY_003237"
     CDS             1486..2139
                     /gene="rluE"
                     /locus_tag="ACHUOY_003237"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR0683945.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2149..2610
                     /gene="nudJ"
                     /locus_tag="ACHUOY_003238"
     CDS             2149..2610
                     /gene="nudJ"
                     /locus_tag="ACHUOY_003238"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR0683946.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2664..3770
                     /gene="mnmA"
                     /locus_tag="ACHUOY_003239"
                     /gene_synonym="trmU"
     CDS             2664..3770
                     /gene="mnmA"
                     /locus_tag="ACHUOY_003239"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR0683947.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3806..4447
                     /gene="hflD"
                     /locus_tag="ACHUOY_003240"
     CDS             3806..4447
                     /gene="hflD"
                     /locus_tag="ACHUOY_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR0683948.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4451..5821
                     /gene="purB"
                     /locus_tag="ACHUOY_003241"
     CDS             4451..5821
                     /gene="purB"
                     /locus_tag="ACHUOY_003241"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR0683949.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
BASE COUNT         1436 a         1573 c         1496 g         1436 t
ORIGIN      
        1 atccacataa ataaaacaac gggcgtgtta tacgcccgtt tcaatattta actcatggag
       61 agattacatg ttttcgatga tcgcttcacc aaactctgaa catttcagca gcttagcgcc
      121 ttccatcaga cgttcaaagt cataagttac ggttttcgcg ttgatagcac cttccatacc
      181 tttaacaatt aaatcagccg cttcagtcca ccccatatgg cgcagcatca tctcagcgga
      241 gaggatgata gagcctggat ttactttgtc ctgaccggca tatttcggcg cagtaccgtg
      301 ggtggcttca aacagggcgc attcgtcacc gatgtttgcg ccaggggcga taccgatacc
      361 gccaacctgc gctgccaggg cgtcagaaat gtagtcaccg ttcaggttca tacaggcgat
      421 aacatcatat tcagccggac gcagcaggat ctgttgcagg aatgcatcag caatcacgtc
      481 tttaatgacg atctctttgc cggtgttcgg gtttttaact ttcagccacg ggccgccgtc
      541 gatcagttca ccgccaaact cttcacgcgc cagctggtag ccccagtctt taaacgcgcc
      601 ttcggtgaac ttcatgatgt tgcctttgtg caccagagtc acagagtcac gatcgttagc
      661 aattgcgtat tcgatcgctg cacgaaccag acgtttggtg ccttcttccg aacacggctt
      721 aataccgata ccacaatgtt ccgggaagcg aattttcttc acccccatct cttcacgcag
      781 gaatttaatc actttctcgg cgtcggcaga gtctgctttc cattcgatac ccgcataaat
      841 gtcttccgag ttttcacgga agataaccat atcggtcagt tcagggtgtt taaccgggct
      901 tggagtgccc tgatagtaac gtaccggacg caggcagatg tagagatcca gttcctggcg
      961 cagggcaacg ttcagagagc gaataccgcc accaacagga gtggtcagcg gacctttaat
     1021 ggcaacgcga tattcacgaa tcagatcaag ggtttcagca ggcagccaga catcctgacc
     1081 ataaacctgt gtggattttt caccggtgta aatttccatc caggagattt tacgctcgcc
     1141 tttataggct ttctcgactg cagcgtcgac cactttcagc atggctgggg ttacatctac
     1201 accgattcca tcaccttcaa tgtaagggat aatcggattt tcaggaacgt tgagtttgcc
     1261 gttttgcagg gtgatcttct tgccttgtgc cggaacaact actttacttt ccattcacct
     1321 ctccttcgag cgctactggt ttgctcgtct gccaccacgt tgcatatgcg tttgcgtcct
     1381 gcgatacgga tgctttagag caattttttg ttaatgattt gtaattggct tgtcaatgct
     1441 accctattgt ttgccgtcat gaaagatgcg cgttattagg ctataatgcg gcaattcata
     1501 atctctgaaa ataccatgca aaaaacttct tttagaaatc accaggttaa gcgattcagc
     1561 tcgcaacgtt ctactaggcg taaacctgaa aaccagccca cgcgtgtgat cctgttcaat
     1621 aaaccctacg atgttcttcc gcagttcacc gatgaagccg gacgcaaaac attaaaagaa
     1681 ttcatcccgg ttcagggcgt ttatgcagca ggtcgtcttg accgcgatac cgaagggtta
     1741 ctggtgctga ccaataacgg cgcgttgcag gcgcgtttaa cccagccggg caaacgcacc
     1801 gggaaaatct attatgtgca ggtggaaggt attcccacac aagacgcact tgaagccttg
     1861 cgcaatggcg taaccttaaa tgatggccct accctgcccg ccggcgcgga gctggttgac
     1921 gaaccagcgt ggttatggcc gcggaatcca ccaattcgtg aacgcaaaag tattcccacc
     1981 agctggctga agatcacctt atatgaagga cgtaatcgcc aggtgcgccg catgaccgcc
     2041 catgttggtt tccccacgct gcgactgatt cgttatgcga tgggtgatta cactttggat
     2101 aaccttgcca atggcgaatg gcgagaagca acagaataag gatgcacaat gtttaaaccg
     2161 cacgttaccg ttgcttgcgt agtgcacgca gagggtaaat ttttagtcgt tgaagagacg
     2221 attaatggta aagcgttatg gaaccaacct gccgggcatc tggaagccga tgaaacctta
     2281 gtggaagctg ccgcccgtga gctatgggaa gaaaccggca tcagcgcgca accgcaacac
     2341 tttattcgta tgcatcagtg gattgcgcca gataaaacgc cgtttttgcg tttcctcttt
     2401 gccattgagc ttgagcaaat atgcccgacc cagcctcatg acagcgatat cgactgctgt
     2461 cgttgggtca gcgccgaaga aattttaaag gcgtcaaatc ttcgttcgcc gctggtggcg
     2521 gaaagtatcc gttgttatca aagtgggcaa cgctatccgc tggagatgat tggcgatttt
     2581 aactggcctt ttacaaaggg tgtcatctaa aaggctgcgt gatagaatac gccgccttga
     2641 agttcaatgt cgtgagtgat ccaatgtctg aaaccgcaaa aaaagtaatc gtcggcatgt
     2701 ccggcggtgt cgattcctcc gtttctgcct ggctgttgca acaacaggga tatcaggttg
     2761 aaggcctgtt tatgaagaac tgggaagaag acgacggtga ggaatattgc acagcggcag
     2821 cggatctggc tgatgcccag gctgtctgcg acaagctcgg cattgaactg cacaccgtta
     2881 actttgctgc cgagtactgg gacaatgtct tcgaactgtt ccttgccgaa tataaagccg
     2941 gtcgcacgcc gaatccggat attctgtgca acaaagagat caaatttaaa gctttcctcg
     3001 aatttgccgc cgaagattta ggtgccgatt atatcgctac cggtcattac gtacgtcggg
     3061 ccgatgtcga tggcaagagc cgcctgctgc gtggtctgga cagcaataaa gaccagagct
     3121 acttccttta tacgctcagc catgagcaga ttgcgcaaag cctgttcccg gtcggcgaac
     3181 tggaaaaacc gcaggtgcgt aagattgctg aagatcttgg tctggtcacc gcgaagaaaa
     3241 aagactctac cggcatttgc ttcattggcg aacgtaaatt ccgcgagttc ctgggccgtt
     3301 atctcccggg gcaaccgggc aaaatcatta ccgtcgatgg cgatgaaatt ggtgagcacc
     3361 aggggctgat gtatcacact ctcggtcagc gtaaaggtct gggtatcggt ggcaccaaag
     3421 aaggcaccga agaaccttgg tatgtggtgg acaaagacgt cgaaaacaac attctggttg
     3481 tcgctcaggg ccatgaacac ccgcggctga tgtctgtcgg gttgattgcc cagcagttgc
     3541 actgggtcga tcgcgaacca ttcaccggca ctatgcgttg cacggtaaaa acccgctatc
     3601 gccagaccga tatcccttgc accgtcaagg cgctggacga tgatcgcatt gaagtgattt
     3661 tcgatgaacc ggttgccgcc gtgacgccgg gccagtctgc cgtcttctat aacggtgaag
     3721 tgtgcctcgg tggcggtatt attgagcagc gtctgccgct gccggtctga ttattatctt
     3781 tacttaaaac aaggaagcag tgaacgtggc aaagaattac tatgacatca ccctcgccct
     3841 ggccggtatt tgtcagtcgg cacgcctggt gcaacaactc gctcaccagg ggcattgtga
     3901 tgccgatgcg ctacacgtct cactcaacag tattattgat atgaacccca gctcgacgct
     3961 ggcggttttt ggcggtagcg aagccaacct gcgcgtcggg ctggaaaccc tgctcggcgt
     4021 gctcaatgcc agcagtcgcc agggcttaaa cgccgaatta acccgctaca cactcagctt
     4081 gatggtgctt gagcgcaaac tctcctcagc gaaaggcgcg ctcgacactc tgggcaaccg
     4141 gatcaacggc ctgcaacgcc agctcgaaca cttcgattta cagtccgaaa cgctgatgag
     4201 cgcgatggct gctatctatg ttgatgtgat cagcccgctt ggcccacgca ttcaggtcac
     4261 cggttcccct gctgtactgc aaagcccaca agtgcaggcg aaagttcgcg caaccctgct
     4321 ggcaggcatt cgcgccgccg tgctctggca ccaggtcggc ggcggacgtc tgcaactgat
     4381 gttttctcgt aatcgcctga ccactcaggc aaaacaaatt cttgctcatt taaccccgga
     4441 gttgtgatct atggaattat cctcactgac cgccgtttcc cctgtcgatg gacgctacgg
     4501 cgataaagtc agcgcgctgc gcgggatttt cagcgaatat ggtttgctga aattccgtgt
     4561 acaagttgaa gtacgttggc tgcaaaaact ggccgcgcac gcagcgatca aggaagttcc
     4621 tgcttttgct gccgacgcaa tcggttacct tgatgcaatt gtcgccaatt tcagcgaaga
     4681 agatgccgca cgcatcaaaa ccatcgagcg taccactaac cacgacgtta aagcggttga
     4741 gtatttcctg aaagaaaaag tggcggagat cccggaactg cacgcggttt ctgaattcat
     4801 ccactttgcc tgtacttcgg aagatatcaa taacctctcc cacgcattaa tgctgaaaac
     4861 cgcgcgtgat gaagtgatcc tgccgtactg gcgtcaactg attgatggca ttaaagatct
     4921 cgccgttcag taccgcgata tcccgctgct gtctcgtacc cacggtcagc cagccacgcc
     4981 gtcaaccatc ggtaaagaga tggcaaacgt cgcctaccgt atggagcgcc agtaccgcca
     5041 gcttaaccag gtggagatcc tcggcaaaat caacggcgcg gtcggtaact ataacgccca
     5101 catcgccgct tacccggaag ttgactggca tcagttcagc gaagagttcg tcacctcgct
     5161 gggtattcaa tggaacccgt acactaccca gattgaaccg cacgactaca tcgccgagct
     5221 gtttgattgc gttgcacgct tcaacaccat tctgatcgac tttgatcgtg acgtctgggg
     5281 ttatatcgcc cttaaccact tcaaacagaa aaccattgct ggtgagattg gttcttccac
     5341 catgccgcat aaagttaacc cgatcgactt cgagaactcc gaagggaatc tgggcctttc
     5401 caacgcggta ttacagcatc tggcaagcaa actgccggtt tcccgctggc agcgtgacct
     5461 gaccgactcc accgtgctgc gtaacctcgg cgtgggtatc ggttatgcgc tgattgcgta
     5521 tcaatccacc ctgaaaggcg tgagcaaact ggaagtgaac cgtgaccatc tgctggatga
     5581 gctggatcac aactgggaag tgctggctga gccaatccag acagttatgc gtcgctatgg
     5641 catcgaaaaa ccgtacgaga agctgaaaga gctgactcgt ggtaagcgcg ttgacgccga
     5701 aggcatgaag cagtttatcg acggtctggc gttgccagaa gaagagaaag cccgcctgaa
     5761 agcgatgacg ccggcaaact acattggtcg cgccatcacc atggttgatg agctgaaata
     5821 aactcgtatc agtgccggat ggcgatgctg tccggcctgc ttattaagat tatccgcttt
     5881 ttattttttc actttacctc ccctccccgc tggtttattt aatgtttacc cccataacca
     5941 c
//
LOCUS       DAZOYL010000236         5899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZOYL010000236 DAZOYL010000000
VERSION     DAZOYL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.237"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            236..859
                     /gene="nfeR"
                     /locus_tag="ACHUOY_003242"
                     /gene_synonym="yqjI"
     CDS             236..859
                     /gene="nfeR"
                     /locus_tag="ACHUOY_003242"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR0683950.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(966..2486)
                     /gene="aer"
                     /locus_tag="ACHUOY_003243"
     CDS             complement(966..2486)
                     /gene="aer"
                     /locus_tag="ACHUOY_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR0683951.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2904..4283
                     /gene="ygjG"
                     /locus_tag="ACHUOY_003244"
     CDS             2904..4283
                     /gene="ygjG"
                     /locus_tag="ACHUOY_003244"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR0683952.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4325..4657)
                     /locus_tag="ACHUOY_003245"
     CDS             complement(4325..4657)
                     /locus_tag="ACHUOY_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR0683953.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            4876..5859
                     /gene="ebgR"
                     /locus_tag="ACHUOY_003246"
     CDS             4876..5859
                     /gene="ebgR"
                     /locus_tag="ACHUOY_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR0683954.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1448 a         1586 c         1475 g         1390 t
ORIGIN      
        1 gataatgatt gttaatcatg ataaatgcaa gcgatttgta gaactgatat gtctatagtc
       61 tgataagacg aaccgcctct tctcaggcat cattactcaa cgccggatgc ggcgtgaacg
      121 ccttatccgg cctacgtgtg agatgagtcc acatttaaaa atcattttta ttcttgcaag
      181 agtgctcata tcagatataa attagatata tctaatcaag caaaaggagg ctgatatgag
      241 ccatcatcac gaagggtgtt gtaaacatga aggccagcca cgccatgagg gctgctgcaa
      301 aggtgagaag tcagaacacg agcactgcgg acacggtcac cagcatgaac acggtcaatg
      361 ctgcggtggt cgccacagtc gcggcggcgg tcgtcggcaa cgtttctttg gtcacggtga
      421 attgcgtctg gtgattctgg atattctctc gcgcgatgac agccacggtt acgaattgat
      481 taaagcgatt gagaatctaa cccaggggaa ttacacccca agcccgggcg tcatctaccc
      541 aacgctggat tttctgcagg agcagtcgct gattaccatc cgcgaagagg aaggtggtaa
      601 gaagcagatt gcgctgaccg aacaaggcgc gcagtggctg gaagaaaacc gcgaacaggt
      661 ggagatgatt gaagaacgca tcaaagcgcg ttgcgttggt gctgcgctgc gccagaaccc
      721 gcaaatgaag cgggcgctgg ataattttaa agcggtgctg gatttacgcg tcaaccagag
      781 cgatatcact gatgcacaaa taaaaaagat cattgcggtg atcgaccacg ccgcttttga
      841 tattacccaa ctggattaat caccgattgc cggatgcggc gtaccctaca caccccgcag
      901 gcctgataag atgcgccagc atcgcatcag gcattgtgct ccaaccgccg gatccggcat
      961 accgattaat gcagcaccgt caccgcgtct tccagtcggc tggcgcggtg tttcaccatc
     1021 gccgacacct gcgcactctc ttccaccagc tcagcatttt tctgggtgat caggttaagc
     1081 tcatccactg cacgggtcag gctggaaagc ccatcggcct gttccagcgt tgaatggcta
     1141 atctgggcga tcaactgggt gacgtttttc acctgtgcca caatatcttc catcgtccgt
     1201 ccggcggcgt gtacctgctg cgaaccggat tgcaccttat cagcactggc atcaatcagc
     1261 ttgcgaatat cgttggcagc attagcgctg cggctggcta aatgacgcac ttcccctgcc
     1321 accactgcaa aacctttgcc ctgttcgccg gcacgcgccg cttccaccgc cgcattcagg
     1381 gccagaatat tggtctgaaa cgcaatatcg ttaatcagcg aagtaatggt gccaatgcgc
     1441 tgggtactgt cggcgatatc gtccattgtc ttgatcaccg tggtcatcgc ctccccaccc
     1501 tgcactgccg cattactggc ggtgatagaa agtttatcgg cagccgacgc cgtggcagag
     1561 ttctgtttca ccgacgccgc catttggttc atggtcgcca ccgtttgctg aacgttatca
     1621 actgtctgct gggtatgttc gttcagttca tcggtgcctt tcgccagcgt ctcactgcca
     1681 tttctgacac tggacacctg gcttgagaca tcgttaatta gccaacggca catcaggcca
     1741 agctgcccta ccgcgcgtaa tgtcagcccc agctcatcgc tgcgattcag gtgctcaaca
     1801 ctattacgct ctccggtcgc caccttcagt gcctgacggg cgacattttc tatcgggcgc
     1861 acaatctgcc attcaaaaca agcgcttgcc aacaatacca ctaacacaca gaggaaatac
     1921 gtcaccaccg gggcagcaac aaaccaaagc atggccgcca gcaagataaa catcagggtc
     1981 atcactccac gcgcccgcca gcgaagcggt aatgaaggca gtttacccag ccagccttta
     2041 cgcaccacca ggcctttatg aatacgctta ctggtacgtc cggcgttcag cgctttgtac
     2101 agcggctcca ccgccgcaat ctcttcatcc gtcgcccggg tacgaatcga catatagcca
     2161 ctgatttttc cctcgcgcac catcggtacc gcattggccc gcacccaata atggtcgcca
     2221 tttttgcggc gatttttcac gatgccgctc cagggctccc cttttttcag ggtgaaccac
     2281 atatccgcaa acgccgcttt tggcatatcc gggtgacgca ccatgttgtg cggctgccct
     2341 tgtaactctt gcaaggtaaa gccgctcacc tgcacaaaag tgtcattagc atgagtgata
     2401 tagctttgca gatcggtagt ggacatcaga gtggtatcgt ccgccagcgg ggtattttgc
     2461 tgggtgacat acggatgaga agacatgttc gcgtcctgtg caggttatat ggttgttaac
     2521 ttcttgtcag agtttatgtc ggccccgctg cggttatctt taaccgatta atttgattta
     2581 gatcgcaatt tgcgattaaa gcacaaatct aattctttga tttaaaatac tttcactctg
     2641 ttactatacg aaaacgttaa ttatcttgcc caaaaatcag gcaattattg ccctgaaaac
     2701 gtgcatttgc gcagcaatca tcaaatccat acccgacaaa aaccgtgcaa aataacaaca
     2761 aatgttaaca gatagcatta aatattgcac aaatgataac cgaatttgtg tttatcccga
     2821 ttttcgcgat cgcagccgga gtggcgcaat ccctgcaata cttaaatcgg tatcatgtga
     2881 tacgcgagcc tccggagcat attttgaaca ggttaccttc gagcgcatcg gctttagcgt
     2941 gcagcgccca cgccctgaat ctcattgaga agcgaacgct ggatcatgag gagatgaaag
     3001 cacttaaccg agaggtgatt gaatacttca aagagcatgt caatccgggg tttttagagt
     3061 atcgcaaatc tgttaccgcc ggcggggatt acggagccgt agagtggcaa gcgggaagtt
     3121 taaatacgct tgtcgacacc cagggacagg agtttatcga ctgcctggga ggttttggaa
     3181 ttttcaacgt ggggcaccgt aatccagttg tggtttccgc cgtacagaat caacttgcga
     3241 aacaaccgct gcacagccag gagctgctcg atccgttacg ggcgatgttg gcgaaaaccc
     3301 ttgctgcgct aacgcccggt aaactgaaat acagcttctt ctgtaatagc ggcaccgagt
     3361 ccgttgaagc agcgctgaag ctggcgaaag cttaccagtc accgcgcggc aagtttactt
     3421 ttattgccac cagcggcgcg ttccacggta aatcacttgg cgcgctgtcg gccacggcga
     3481 aatcgacctt ccgcaaaccg tttatgcagt tactgccggg cttccgtcat gtgccgtttg
     3541 gcaatatcga agccatgcgc acggctctta acgagtgcaa aaaaaccggt gatgatgtgg
     3601 ctgcggtgat cctcgaaccg attcagggtg aaggtggcgt aattctgccg ccgccgggct
     3661 atctcaccgc cgtacgtaag ctatgcgatg agttcggcgc actgatgatc ctcgatgaag
     3721 tacaaacggg catggggcgc acgggcaaga tgttcgcctg cgagcatgag aacgtacagc
     3781 cggatatcct ctgccttgcc aaagcgctcg gcggcggcgt gatgccgatt ggcgcgacca
     3841 tcgccactga agaggtgttt tcagttctgt tcgacaaccc attcctgcat accaccacct
     3901 ttggcggcaa cccgctggcc tgtgcggcgg cgctggcgac catcaatgtg ttgctggagc
     3961 agaacttacc ggctcaggct gatcaaaaag gcgatatgtt gctggacggt ttccgtcaac
     4021 tggcgcggga atatcccgat ctggtacagg aagcgcgtgg taaagggatg ttgatggcga
     4081 ttgagtttgt tgataacgaa atcggctata actttgccag cgagatgttc cgccagcgcg
     4141 tactggtggc cggaacgctc aataacgcca aaacgatccg cattgaaccg ccactgacac
     4201 tgaccattga acagtgtgaa ctggtgatca aagcggcgcg taaggcgctg gcggccatgc
     4261 gagtaagtgt cgaagaagcg taatatcaaa tcggatggcg atacgacgtc gccatccgat
     4321 ctttttatac gacgcgtacg cccgcaggca tcatccgctc cggcgttaac agcacgcttt
     4381 cactgccgtc atcggtttcc gcacacaaca gcatgcattc cgacgtttca ccacgcattt
     4441 tcgctttttg cagattgcat aagaccacca ccgtttttcc catcagctcc tcttcgctgt
     4501 agtacggcac caggctggtc acggtttgca gtgttttttc ccccacatca acctgtacga
     4561 tgtacagctt gtcggcgttt tcatggcgtt tcacttccac aatctttccg acgcgcattt
     4621 ccagacgtgc aaaatcagcg taagccacgg tttccattgc ttcctccctt caagtaaaat
     4681 tttactaaac tatagcaaaa ttttttctca atcctgtagg ctaaaaatgg agaatgcagg
     4741 cgtgatcaca ttcgtaagcc gctgtgttac cgttacagcg tcaaagaaac gcgctttatt
     4801 tactgaaaac aggtgacccg ataagcactt cctctacaat gggggcgcac atcagggaaa
     4861 gtaaaaaagg taaacatggc aacattaaaa gacatcgcaa tcgaagctgg cgtatccctg
     4921 gcgacagtat ccagggtctt aaatgacgat ccgacattga atgtgaaaga agagacgaaa
     4981 catcgcattc tcgagatcgc cgaaaagctg gagtacaaga ccagtagcgc ccgtaaacta
     5041 cagacaggcg cagtcaacca acaccatatt ctggctatct acagctacca gcaggagctg
     5101 gagatcaacg atccttacta tctggcgatc cgccacggca ttgaaactca gtgcgaaaag
     5161 ctggcgatcg aactgaccaa ctgttatgaa cacaacggat taccagacat taaaaacgtc
     5221 accggtattt taattgtcgg caaacccacg cccgccctgc gcgccgctgc cagcgcgttg
     5281 accgacaata tctgttttat cgactttcac gaacccggca gcggttacga tgcggtggat
     5341 atcgatctgg cacgcatcag taaagaaatc atcgacttct atatcaacca gggcgttaat
     5401 cgtattggtt ttattggcgg tgaagatgag cctggcaagg cggatattcg tgaggtcgcc
     5461 tttgcggaat atggccgact gaaacaagtg gtacgcgaag aggatatctg gcgcggcggt
     5521 ttttccagtt cgtcgggtta tgaactggca aaacaaatgc tggcgcggga agactatccg
     5581 aaggcactgt ttgttgcttc cgattccatt gctatcggcg tactgcgggc aattcatgaa
     5641 cgtggcctga acatcccaca ggatatttcg cttttcagcg ttaacgatat ccccaccgcg
     5701 cgatttacct ttccgccgct ctccaccgtg cgcatccatt ccgagatgat gggaagtcag
     5761 ggcgttaacc tggtgtatga aaaagcccgc gatggtcgcg cgctgccgct gctagtcttc
     5821 gttcccagca aattaaaact gcgcggcacg acccgttaaa tccccttaca cactgtccgg
     5881 caatcatttt tgccggaca
//
LOCUS       DAZOYL010000237         5888 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZOYL010000237 DAZOYL010000000
VERSION     DAZOYL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.238"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..359)
                     /locus_tag="ACHUOY_003247"
     CDS             complement(<1..359)
                     /locus_tag="ACHUOY_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HFR0683955.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVAT"
     gene            complement(504..1007)
                     /gene="cheW"
                     /locus_tag="ACHUOY_003248"
     CDS             complement(504..1007)
                     /gene="cheW"
                     /locus_tag="ACHUOY_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR0683956.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1028..2992)
                     /gene="cheA"
                     /locus_tag="ACHUOY_003249"
     CDS             complement(1028..2992)
                     /gene="cheA"
                     /locus_tag="ACHUOY_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR0683957.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(2997..3923)
                     /gene="motB"
                     /locus_tag="ACHUOY_003250"
     CDS             complement(2997..3923)
                     /gene="motB"
                     /locus_tag="ACHUOY_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR0683958.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3920..4807)
                     /gene="motA"
                     /locus_tag="ACHUOY_003251"
     CDS             complement(3920..4807)
                     /gene="motA"
                     /locus_tag="ACHUOY_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR0683959.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4933..5511)
                     /gene="flhC"
                     /locus_tag="ACHUOY_003252"
     CDS             complement(4933..5511)
                     /gene="flhC"
                     /locus_tag="ACHUOY_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR0683960.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5514..5864)
                     /gene="flhD"
                     /locus_tag="ACHUOY_003253"
     CDS             complement(5514..5864)
                     /gene="flhD"
                     /locus_tag="ACHUOY_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR0683961.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         1377 a         1587 c         1434 g         1490 t
ORIGIN      
        1 ctggtagcga ccatttcagg taacggtgcc atgcttttga attttttata atgcgtcgct
       61 gcctgcgcca atgttttcct ggcgctatcg agcaattcaa ctttggcgtt actttgttga
      121 ttggaggaat ccatcatcat ccgcaccgct gaacgactca ggttaatgcg cgtttgcagc
      181 attaaatccc aggttgacgt cagctcgccc tgctgttccc gcaattgatt ggaaaccaca
      241 aagctcttct ggctatggtg aagggaagaa aaaaacagac tgccggaaat aagctgtaac
      301 agtgcgaata cccccagcac cattaccaac agcgtgacta cgcggatacg gttaatcata
      361 aggcaccttc ctgaaaacaa gttgatctcg ttatcggcaa gaagggggga aactttattg
      421 ctgatgccac ccgccgcgaa attgaaataa aaaacccgat gcgcagatca tcgggttcat
      481 ttcaattgag gaaatcggga gaattacgcc acttctgacg ccgcgctatc taacagcgcc
      541 atctcttcgc tgttcagcag tttttcgatg ttcaccagaa tcaacatacg gtcgcccagt
      601 gcgcccagtc cagtgagata ttctgttgaa agcgtcacgg caaactctgg tgccggacga
      661 atttgctccg ccgtcaatga aagcacgtct gaaacgccgt caaccacgat gccgaccacc
      721 cgctgtccga gattcaggac gataactacc gtgttgtcgt tatagtccac atccacctgg
      781 ctgaacttaa ttcgtaagtc aacaatcggc acaataacgc cgcgcagatt cgtgacgcct
      841 ttgataaacg ctggcgtgtt cgcaatccgt gttacctgat cgtagccacg gatctcctgc
      901 actttcagga tatcaatacc gtactcttca tcaccaaggg taaataccag aaattcctgg
      961 cctgacggct cgctggccag ctttgttaca ttcgtcatac cggtcatatt gttacctttt
     1021 tactcattca ggcggcggtg ttcgccatac gttgttcgcg gtttatcgcc tgcaaggcgg
     1081 agacatcaac aatcagtgcc acgctgccgt cgccaagaat ggtcgcagca gaaatgccgg
     1141 ggactttgcg atagttactt tcaaggtttt taaccacaac ctggtgttga ccaattaatt
     1201 gatccaccag caaggcgtag cggcgaccgc cactttgtaa gatcacaaca attccctggg
     1261 tggcttcggt tttcgcgccc gcgacgttga acactttcca cagttcgacg atgggcagat
     1321 attcaccccg cacttccagt acccgctcgc cgccggccag tggatggaga tcggcttcac
     1381 ggggttgcag tgattccata acagcattca gcggcagaat gaaaacttca tccccaacgc
     1441 gtacggacat gccgtcgagg atggccagcg tcagcggcag taaaatgcgg atcgtagtgc
     1501 cagtcccctg cttcgactgg atttcgacat gaccgcccat ctcctggata ttacgtttaa
     1561 cgacgtccat gccgacgccg cgcccggaga cgtcggtgac ctgctctgcc atggagaagc
     1621 ctggtgcaaa tatcagcatc gcgacttcgt cgtcgctcat gttttcgctg acagtcaaac
     1681 cttgcgaggc cgcttttgcc agaattcgct cacggtttag ccccgcccca tcgtcggtca
     1741 cttcaatgca aatgttgccg ccctgatgtt cggcagacag aattaaattt ccgacgctgt
     1801 ttttacctgc ggcgagccgt ttttctggca gttcaatacc gtgatcgagg ctattgcgta
     1861 ccaggtgggt cagcgggtcg ataatgcgtt ctatcaggct tttgtcgagt tcagtagaac
     1921 tgcccaccag cgtcagttct acctgcttgc cgagttttcc cgccagatca cgcaccagcc
     1981 ggggatagcg actaaaaaca tattccatcg gcatcatgcg aatcgacatc actgattcct
     2041 gcaaatcacg ggcgttacgt tgtaactgcc ccatgctggt tatcaaatca ccatgattaa
     2101 ccgggtccag ttcgctggaa cgctgggcaa gcatggactg ggtgataacc agctcgccga
     2161 cgaggttaat taattgatca accttttcta ccgctacacg gatgctggtg gattcattgc
     2221 tgcgcgtcgt tttttcccgc tccacgcggc cggttggcgc ttgttcggct gccagtttaa
     2281 gcactggtgg ggtggatatt tttggcgaga cttctactgt ttcaaaggta atctgatcgg
     2341 cttcaatcac aaaacagagt accgctgtga tgtcatcttc ggcgatgtcg cccggtaata
     2401 ttgccgagag cgaatccgcc cctttcacca cgtcagttaa cgttgtcaga tgtcccagtt
     2461 cttcttccag caggtcgact tccccggcct tcaggcgcga aaggataatt cgtcgcggcg
     2521 actgactgcg actctgctca tcttgcggtt cacttttggc aaccacactt aatcgggtca
     2581 ctgcggatgg cgtttcgcct ttcgcttcta atgccagttg acgcaaggcc tggcagatat
     2641 aatcgaagct ggcggcatcc ggctcttgcg actgtttata agcgtcgagc tgttcttgca
     2701 tgatgtcctt cgtttccaaa aacagattga taatgtcggt gttgagttgc atctcacctc
     2761 gtctggcttc atcgagcagg ttttccatca gatgcgtggt ttcctgcaaa acgctgaagc
     2821 caaaagttcc tgcccctcct ttgatcgagt gggcagcccg aaagatggca ttcaattgtt
     2881 cggcatctgg cgcttccggc tgcaaaacca gcagatgctg ctccatgtca gccaacagtt
     2941 cgtccgcttc atcaaaaaat gtctgataaa aatcgcttat atccatgctc acgctgtcac
     3001 ctcggttcgg ctgatggcat tgtgggaaca ctgacctgtg gtgcaacctc aggtttttcc
     3061 agggcgctta ctggctcatt ctggctttcg gcgttttcat gcaaaatggc ctgttcggct
     3121 tgtttgttca gtaccagcag gctgatgcga cggttgacgg catcatcagg tccgcgatcg
     3181 cttaagcgca tcgttgccgc catgccgacg acacgtaaca ctttgccgct atccaaccct
     3241 ccgaccatca gttcgcggcg ggatgcattg gcccgatcgg cagaaagctc ccagttgcta
     3301 tatcctttct caccgctggc gtaggggaaa tcatcggtat gacctgaaag gctaatacgg
     3361 ttgggaatac cgttcagtac aggcgcaatg acacgcagaa tgtcgcgcat atagggttcg
     3421 acatcggcac tgccggttct aaacatcggg cgattctggc tatcgatgat ctgaatacgt
     3481 agaccttcct ggaccagatc gattttgaga tggggacgta acgcccgcag tttcggatcg
     3541 gactctatca actggtcgag atcaccccgc aatttccgca atcgactttg ctccatgcgt
     3601 tttttcagat cttcgatgtt cggctgctta ttcacttccc cctggctttg ggtgtaatca
     3661 tcaccaccgc cgggaattgg gctttcacta ttagaaatgc gatcgccgcc cgtaaccgca
     3721 gtcgccagtg gagtccggaa gtactccgca atctgaatca gctcttttgg gctggagatg
     3781 gagatcagcc acatcaccag aaaaaaggcc atcatcgcag tcataaagtc ggcataagca
     3841 atcttccacg atccatgtgc tgccccgtgg cttttggctt tgcgtcgttt gacgacaata
     3901 atcggatgcg cttgattctt catgcttcct cggttgtcgt ctgttgttgc ggatttttca
     3961 ccgcacgcac atgctcttcc agttcaataa acgacggacg ttcgctggaa tagagcgttt
     4021 tgcgaccaaa ctcaacggcg ataggcggtg cgtaaccgtt cagattagaa agcagagtga
     4081 ctttgacgca ctgcatcatt ttgctggttt cggcgctttt ctgacgtaaa acactcgcta
     4141 atggggaaat aaatccgtaa gccaataaaa tgccgaggaa agtccccacc atcgcatgtg
     4201 cgataagcgc acccagctcg gcggcaggac gatcggctga acctaacgcg tgaacgcccc
     4261 ccattacagc cgcgacaata ccaaacgccg gaagtgagtc cccgaccagc gccagactgt
     4321 tcgccgggac ttctgcctcg ctttcgtgcg tctcaatctc ttcatccatc agggcttcga
     4381 tttcgaaggt gttcatatga ccgctgataa tcagacgcag ataatcgacg ataaaatcaa
     4441 gcatgacgct atccgcgagg atgcgtgggt agctggcgaa gatctcgctc tcacggggat
     4501 tttcaatatc acgttccagc gaaaacatcc ccatctgccg cgatttcgcc atcaaccgat
     4561 aaagcagagc cagcagatcc atatacattg ctttggtgta tttggagcga cgaaacagca
     4621 acggcagcgc cttcagcgtg cctttaatcg ctttgccatt attgccgacg ataaacgacc
     4681 caatccctgc accggcaata atcaccagtt cagcgggttg atagagtgct ccaaggcttc
     4741 caccggtcat caaataaccg ccgaaaactg taccgagaac aaccaggtaa cctaataaga
     4801 taagcacgac atcatccttc cactgttgac catgacagga tgttcagtcg tcaggcgtta
     4861 acgcgcgatt ggggcaaaaa aagcagcggt acgtcgttac cgctgctgga atgttgcgcc
     4921 tcaccgtatc agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc
     4981 ggcaggattc tgggaaagtt tacgtctttt tactgcccgg gatggcggtt gacataagct
     5041 gcaggcaaag ctgccaacag gctggtgagc gtgggtaata aaattgccgc cgcagcagtt
     5101 gcagctggaa agttgcagta atccactttc aacaaaccgc accaatgtcc aggcacgggt
     5161 taatgccagc agtggtcctt cttctgcttg tgggcactgt tcaaggtata aacggtaggc
     5221 tttgatcacc gcatcgacgc cattacacaa accggttttc agtaaaaact gccatgcatt
     5281 acagaacatc gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg
     5341 cagcatgcct ttcggcggtg ggcttccgcg cagttcttta taaagtttta tcaggcgtcc
     5401 gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg taatcaattc
     5461 cattgccagc tgaatatccc gcgcttcctg aacaatgctt ttttcactca tgatcaggcc
     5521 cttttcttgc gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg tgttgagagc
     5581 atgatgccgg tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt caactgagta
     5641 atcgtctggt ggctgtcaaa acggaagtga caaaccagtt gattggtttc tgccagctta
     5701 accatttgcg gaagagtcag tgccgctaac gttgtcgcca tttcttcatt tatgccgaga
     5761 cgaaacatag cggacgcttt gtcctgaaca atcaaacgct gtgcaagtag taaatatgac
     5821 aagttgatgt cataaatgtg tttcagcaac tcggaggtat gcattattcc cacccagaat
     5881 aaccaact
//
LOCUS       DAZOYL010000238         5854 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZOYL010000238 DAZOYL010000000
VERSION     DAZOYL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.239"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..225
                     /locus_tag="ACHUOY_003254"
     CDS             <1..225
                     /locus_tag="ACHUOY_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR0683962.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            271..621
                     /gene="traA"
                     /locus_tag="ACHUOY_003255"
                     /pseudo
     CDS             271..621
                     /gene="traA"
                     /locus_tag="ACHUOY_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            636..947
                     /gene="traL"
                     /locus_tag="ACHUOY_003256"
     CDS             636..947
                     /gene="traL"
                     /locus_tag="ACHUOY_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR0683963.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            969..1151
                     /locus_tag="ACHUOY_003257"
                     /pseudo
     CDS             969..1151
                     /locus_tag="ACHUOY_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
     gene            1153..2403
                     /locus_tag="ACHUOY_003258"
                     /pseudo
     CDS             1153..2403
                     /locus_tag="ACHUOY_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2400..2786
                     /gene="trbI"
                     /locus_tag="ACHUOY_003259"
     CDS             2400..2786
                     /gene="trbI"
                     /locus_tag="ACHUOY_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR0683964.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2783..3094
                     /locus_tag="ACHUOY_003260"
                     /pseudo
     CDS             2783..3094
                     /locus_tag="ACHUOY_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
     gene            3089..4087
                     /locus_tag="ACHUOY_003261"
                     /pseudo
     CDS             3089..4087
                     /locus_tag="ACHUOY_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            4107..4854
                     /gene="traX"
                     /locus_tag="ACHUOY_003262"
                     /pseudo
     CDS             4107..4854
                     /gene="traX"
                     /locus_tag="ACHUOY_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            4909..5469
                     /locus_tag="ACHUOY_003263"
     CDS             4909..5469
                     /locus_tag="ACHUOY_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0683965.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5599..5811
                     /locus_tag="ACHUOY_003264"
     CDS             5599..5811
                     /locus_tag="ACHUOY_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0683966.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1384 a         1447 c         1797 g         1226 t
ORIGIN      
        1 gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc ggaggtaata
       61 caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac gtgggtggac
      121 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      181 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
      241 actctccagg aaacccgggg cggttcagcg ttaagtgttc agggtacttc tgcgcccgtc
      301 aaaaagaaat cgtttttttc taaattcact cgtctgaata tgcttcgcct ggttcgtgca
      361 gtgatcccgg ttgctgttct gatgatgttg ttccctgagt tagctatggc tgctggtaaa
      421 ggcacggacc tgatggctaa aggtcaggaa acggttaagg cgaccttcgg taaggactcc
      481 agtatcgtta aatgggttgt tctggctgaa gttctggtcg gtgcagtcat gtacatgatg
      541 accaaaaacg tcaagttcct ggtcggcttt gccatactct ctgtatttat tgcagtaggt
      601 atgtctgttg ccggattctg acaggaaata aaacgatgtt gggagacgag aataaactta
      661 agaaatatcg tttcccggaa acactgacca accagagccg ctggtttggc ctgccactgg
      721 atgaactgat ccccgcagca atctgtgttg gctggggtat cacaacatcg aaatatctgt
      781 tcggtattgg tgcagcggtt ctggtttatt tcgggattaa aaaactgaaa aaagggcggg
      841 gcagttcctg gttacgtgac ctgatttact ggtatatgcc aacagccctg ctgcgcggta
      901 tttttcataa tgttcccgat tcgtgtttcc ggcagtggat taaatagaac tgataccagg
      961 attgttatat ggaacacggt gcccgtttaa gtaccagtcg tgtaatggcc atcgccttta
     1021 tatttatgtc agtgcttatt gttctcagcc tctctgttaa cctcattcag ggggtgaata
     1081 actaccgtct tcagaatgag caacgcactg ccgtgacccc aatgggattt aatgtgccct
     1141 ttgccgtgtc acacctaccg taaccagctg gcgtttatcg atattttctt ccggggaatg
     1201 aataacacca actacaacat ggcggtctgt ggcacctccg gggccggtaa aaccgggctg
     1261 atacagccac ttatccgcag cgtgctggac tccgggggct ttgccgtggt gttcgacatg
     1321 ggggatggct acaagtccct gtgtgagaac atgggtggcg tatatctgga cggtgaaacc
     1381 ctgcgtttta acccgtttgc gaacatcacc gatatcgacc agtcagcgga gcgtgtccgt
     1441 gaccagttgt cagtgatggc cagccccaac ggtaacctgg atgaagtgca tgaaggtctg
     1501 ctgctgcagg cggtcagggc ctcctggctg gccaaagaga acagggcacg tattgatgac
     1561 gtggtggatt tcctgaaaaa cgccagtgac agcgagcagt atgccgggtc accgactatc
     1621 cgcagccgtc tggacgaaat gattgtgctg cttgaccagt acactgccaa cggcacttac
     1681 ggccagtatt ttaactctga tgagccgtcc ctgcgggatg acgcaaaaat ggtggtgctg
     1741 gagctgggcg gactggaaga ccgtccgtca ctgctggttg cggtgatgtt ctccctgatt
     1801 atctacatcg agaacaggat gtaccgcacg ccgcgtaacc tcaagaaact gaacgtcatt
     1861 gatgaaggct ggcgtctgct ggacttcaaa aatcacaagg tcggtgagtt tattgagaaa
     1921 ggttaccgta ccgcccgtcg tcataccggt gcttatatca ccatcacaca gaacatcgtc
     1981 gactttgact ctgacaaggc ctccagtgcg gcccgtgcgg catggggtaa ctcctcctac
     2041 aaaattatcc tcaaacagag tgcgaaggag ttcgcaaaat acaaccagct gtatccggac
     2101 cagttccagc cactgcagcg cgacatgatt ggtaagtttg gtgcggccaa agaccagtgg
     2161 ttcagttcct tcctgctgca ggtggaaaac cattcctcct ggcaccgcct gtttgtggac
     2221 ccgttaagcc gcgccatgta cagctctgac ggcccggatt ttgagtttgt gcagcagaaa
     2281 cgtaaagagg ggctgagtat tcatgaggcg gtgtggcagc tggcgtggaa gaagtcaggg
     2341 ccggagatgg cttcgctgga agcctggctg gaagaacatg agaaatacag gagtgttgca
     2401 tgaccacaac gcaaaaaacg actgatgtta cagccccccg acgcagccac tggtggtgga
     2461 cggtgccggg atgcctggca atggtgttac tgaacgcagc cgtcagttac ggcattgtca
     2521 gactgaacgc accgatgact gccgccttca acatgaaaca gactgtggat gcattttttg
     2581 acagcgccag tcagaaacaa ctgtcagaag cacagtcaaa agccctttca gcgcgtttta
     2641 acacggcact ggaagccagc ctgcaggcgt ggcagcagaa acaccacgcg gtcattctgg
     2701 tgtcgcctgc cgtggtacag ggggctccgg atatcacccg ggaaatccag caggatattg
     2761 cccggcgaat gagagcggaa ccatgagatg ccgggggctg attgccctgc tgatatgggg
     2821 gcagagtgtg gccgccgccg atcttggcac ctggggtgat ctgtggccgg taaaggagcc
     2881 ggacatgctg acggtgatca tgcagcgcct gacggcactg gagcagtccg gtgagatggg
     2941 ccggaaaatg gatgccttta aggagcgggt gatccgcaac agtctgcggc cccctgccgt
     3001 gcccggtatc ggacgcacgg agaaatacag cagccggttg tttgacccgt ccgtcagact
     3061 ggctgcggat atccgggata acgaagggcg ggtggccggc tttgagtcag cctacgtggc
     3121 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     3181 tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga
     3241 ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc
     3301 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt
     3361 tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa
     3421 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     3481 gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca
     3541 gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc
     3601 ccgtgataat cctgacagtg gtgtggtggt aagcattgcc ggtgaaggcc gtccgtggaa
     3661 tcccggtgcc atcaccgatg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc
     3721 gggagccgga aatggcgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     3781 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     3841 caaacctgac ctgccggacg gcaaaacaga gctggctgtc agggaaattg ccgggcagga
     3901 gcgtgaccgg gctgccataa ctgaacggga agccgcgctg ccggagagtg tgctgcgtga
     3961 atcacaacgg gagcgggaag cggtccggga ggttgcccgg gaaaatctgc tgcaggagcg
     4021 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg
     4081 agactgatac aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg
     4141 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     4201 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     4261 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg
     4321 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     4381 ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc tttccctggt
     4441 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     4501 cgcgcagtgg gtggcgtaca gcagcggcca ttcttctgat ggccctgttg ggggcctttg
     4561 tccggcacca gttacggcat tgccgggctg ctgatgctgg cagtcagcca ccgactgtac
     4621 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     4681 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     4741 accgttggtc tggtgtcgtg tgcaggaaaa tcattacccc gtttctggcc cggggatttt
     4801 ttcccggtat tctacgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     4861 tggtatcttt gaccgtaaca ggatgattcg tcagaggcag aacacagcat gacagagcag
     4921 aagcgaccgg tactgacact gaagcggaaa acggaagggg agacgcctgt ccgcagccgg
     4981 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     5041 gagaaagcag cccgggaagc agagctggcg gcaaaaaaag cgcaggccag acaggcactg
     5101 tccatttatc tgaacctgcc cacgctggat gaggccgtga ataccctgaa accctggtgg
     5161 ccgggattat ttgacggtga caccccccgg cttctggcct gcggtatccg ggacgtgtta
     5221 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     5281 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     5341 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aagaagcgta tgcggcagcg
     5401 cgtctggata aaatccgccg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     5461 gagaaataaa aaaaagcctc catcagagag gaggcaggga aataaggctt aaaaggaaat
     5521 aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa
     5581 ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg
     5641 cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa
     5701 gcggcagaag catggcgacg ggctgcctgt gttgcgccca ggacagactg gcagcagttt
     5761 gcccgaaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag
     5821 cagccttcat tactccagga gtcagaaggc tgaa
//
LOCUS       DAZOYL010000239         5853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZOYL010000239 DAZOYL010000000
VERSION     DAZOYL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.240"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..2199
                     /locus_tag="ACHUOY_003265"
                     /pseudo
     CDS             1..2199
                     /locus_tag="ACHUOY_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2192..3328
                     /locus_tag="ACHUOY_003266"
     CDS             2192..3328
                     /locus_tag="ACHUOY_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0683967.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="ACHUOY_003267"
     CDS             3325..5325
                     /locus_tag="ACHUOY_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR0683968.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..>5853
                     /locus_tag="ACHUOY_003268"
     CDS             5447..>5853
                     /locus_tag="ACHUOY_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR0683969.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGIT"
BASE COUNT         1351 a         1562 c         1594 g         1346 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cac
//
LOCUS       DAZOYL010000240         5835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZOYL010000240 DAZOYL010000000
VERSION     DAZOYL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.241"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(124..558)
                     /gene="uspA"
                     /locus_tag="ACHUOY_003269"
     CDS             complement(124..558)
                     /gene="uspA"
                     /locus_tag="ACHUOY_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR0683970.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            949..1284
                     /gene="uspB"
                     /locus_tag="ACHUOY_003270"
     CDS             949..1284
                     /gene="uspB"
                     /locus_tag="ACHUOY_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR0683971.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(1528..3027)
                     /gene="pitA"
                     /locus_tag="ACHUOY_003271"
     CDS             complement(1528..3027)
                     /gene="pitA"
                     /locus_tag="ACHUOY_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR0683972.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            3259..4461
                     /locus_tag="ACHUOY_003272"
     CDS             3259..4461
                     /locus_tag="ACHUOY_003272"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR0683973.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLFEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(4777..5760)
                     /locus_tag="ACHUOY_003273"
                     /pseudo
     CDS             complement(4777..5760)
                     /locus_tag="ACHUOY_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT         1528 a         1448 c         1485 g         1374 t
ORIGIN      
        1 ttttgattgc gtacgcagat cgtaggccag ataaggggtt tacgctgcat caggtaacaa
       61 ggcgcgccaa aaagcaaaac gcccgcatat gcgggcgttc aggaacacgt cgtgggggga
      121 tgattattct tcttcgtcgc gcagcggaac aatcagcata tcaacgtgaa cggtgttgat
      181 cagctgacgt gcggaagaca ttagtttgct ccagaagtcc tggtggtgac cacaaaccac
      241 cagatccata tcgtatttct tgattgcatc gaccagaacc tggcccaggt cgccgctgcc
      301 gctcagggtt tcagtgattg ggtagcctgc attagtggaa agctcggtca gcgcatgatg
      361 tgtctcttca gagatgcgtt tctgcatatc acccagattc acatcaataa gcccggtgta
      421 taggtcagag tagtttacat ctacgtggat cagagaaact ttcgcattgt aggggcgagc
      481 catagagact gctttctcta ccagaacttt gctttccggg gagagatcga ccgcgatgag
      541 aatgtgttta taagccatag tgttactcct tccataaagt tgtcgatgac tggccagcta
      601 gcgtttcttg tgccgttacg tcacccgcgt cctgcgaact acgtgcgcgg ggctaaccca
      661 gccgtaaaga ttcagtgtta agaccatcct taccttatag cgacccggat gatccgtcaa
      721 tccgccttgc ttacccgtta gataaacaaa ttttcgacat gaatatattg atagtggtta
      781 accttctgga aaaaaaacaa cctgatctcc tacactatct atagagccgc tcggatgttg
      841 gtcaccacaa aagcggtttc tccaggacct ggtgttaact gattggcagc gtatcgggag
      901 cggtagcccc ggggtggaac tccgcgggca ggtcgccggg gaggaggtat gataagcacc
      961 gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta acatggcgcg ctatttctca
     1021 tcactacgcg cgttgttagt ggtactgcgt aactgcgatc cattgctcta tcaatatgtt
     1081 gatggagggg gcttttttac ctcacatggc caacccaaca aacaggtgcg tctcgtttgg
     1141 tatatctatg cccaacgtta tcgcgatcat cacgatgatg aatttattcg ccgctgtgag
     1201 cgggtgcgtc ggcagtttat tctgaccagc gcattgtgtg gtctggtggt ggtcagcctg
     1261 attgcattga tgatttggca ttaagcactt cagcaataat aacgcataaa aaaagcgggc
     1321 cagtaaactg acccgctcga gactgattac aaacgcaaaa ttgcctgatg cgctacgctt
     1381 atcaggccta cataatctct gcaatctatt gaatttgcat gtttttgtag gccggataag
     1441 gcgttcacgc cgcatccggc atgaacaaag cgcactatgt cacaatctga agcgggccag
     1501 ctgacccgct cgttttggtg cgtacgatta caggaactgc aaggagagcc agtacagccc
     1561 gccggaaagc agtaccgcag ccggaagggt aaacacccag gccatcagaa tactggttac
     1621 ggttttacgc tgtaagccgc cgccatctac caccatcgtc cccgcgacag aagaggagag
     1681 tacgtgagta gtggaaaccg gcatcccggt ataactcgcc aggccgatag acactgccgc
     1741 cgtcatctgg gcagacatcc cctgagcgta ggtcatgcct ttcttaccga ttttctcacc
     1801 gatagtcgtt gccacacggc gccagccaat catcgtaccg atacctaacg ccagcgcgac
     1861 cgccatgatg atccacaccg gtgcatactc gatggtgcta agcatgtcgg acttcagttt
     1921 cttcaacagg cgctgatcgt cagcactcac gccaggcatc ttcaccactt tgtcgatagt
     1981 gtcagaaacg cacagcataa tgcggcgcat ctggctacgt tgatcaagcg acagcttgtc
     2041 gtagctttcc acatcggtgg tcaacatacc tttcaggcgg ttgagcgcgt taatggtatt
     2101 cgacggatgg cagtggaact ccgcaggttg cgttgcgcca gcttccggag ccggtactaa
     2161 ctgatcagca ccggtagcct gtttgagcag cgcaggatgc tgctcaaagt aagcttcgac
     2221 gttgttgatg gcatcacggg tacgggtgat ttcgtagcca gtggcattca tgttcaccac
     2281 gaagcctgct ggcgcgacgc caatcaatac caacataacc agaccaatgc ctttctgacc
     2341 atcgttcgcg ccgtgcgaaa acgccacgcc gatagcagaa aggatcagcg cgatacgtgt
     2401 ccagaacggc ggctttttct tgccgtcttt cttttcacgc tccgctgggg tcaggtggat
     2461 acgggcgcgt ttcttggtgc cgctccagta gcgacgcagc aagaaaatca gaccgccagc
     2521 aaacaccagg ccgacaatag gggaaacgat cagagaaccg aaaatactta atactttcgg
     2581 gatattgagt gcatccacca ctgacgtccc ggtcatcaac gcattggtta aaccaatccc
     2641 gatgatcgcg ccaatcagcg tatgagagct ggatgcaggt aaaccaaagt accaggtacc
     2701 caggttccag ataatcgccg ccagcaacat agagaacacc atggcaaggc catgagacga
     2761 tcccatatta agcagcagat ccgtcggcag catatgcaca atggcatagg caacactcag
     2821 accacccagc aaaacaccca aaaagttgaa caccgccgcc ataaccacgg cgagctgaga
     2881 acgcatcgcg cgggtataga taacggttgc cacggcgttg gctgtgtcat ggaaaccatt
     2941 gatggcttcg tagaacagca caaaagccag tgcaagcaat aataacagcc cggtatgcaa
     3001 atccaggcca gcaaacaaat gtagcatagg acgttacgcc attttgagga catgaacgcg
     3061 gcgcattatc agtgactttc gcggcgcggg caaagtgaaa tatagacttt ttttgatttg
     3121 cctcctgtat ggatttcact caaaaaataa ttatcttata taattcaggc aaatacttcc
     3181 ttttagtaat attgatgctg gtgcgaccac tgaggaatct ttacaattca cgcccgtttt
     3241 ttctaagagg agcgcaacgt ggaaaggttt gatgccatta ttataggcgc tggtgcggcg
     3301 ggtatgttct gttctgcgct ggcaggtcag gcaggacgcc gggttctgct gatcgataat
     3361 ggtaaaaaac cagggcgcaa aatccttatg tctggcggtg ggcgctgcaa ctttaccaac
     3421 ctttatgtcg aaccaggcgc ttatctgagc cagaatccgc atttttgtaa gtctgcactc
     3481 gcacgtttta cccagtggga tttcattgat ctggtcaata aacacggcat cgcatggcac
     3541 gagaaaacgt tagggcaact cttctgcgat gactccgcgc agcagattgt cgacatgctg
     3601 gtggatgagt gcgagaaggg caatgtgacc ttcagattgc gtagcgaagt gctgagtgtg
     3661 gcggaggatg aaacaggctt cacgcttgat ctgaacggca tgactgtcgg ttgcgaaaag
     3721 ctggtcatgg cgaccggtgg gctgtcaatg ccggggctgg gcgcgtcgcc gtttggttat
     3781 aagattgccg aacaatttgg cctcaacgtg ctgccgaccc gtgcgggtct ggtgccattc
     3841 actctgcata aaccgttgtt cgaagagtta caggtgctgg cgggcgtggc ggtgccttcc
     3901 gtgattaccg ctgaaaacgg catcgttttc cgtgagaact tactcttcac ccatcgcggc
     3961 ttgtctggac cggcggtgtt gcagatttcc agctactggc aaccgggtga atttgtcagt
     4021 atcaatctgc taccggatgt ggatctcgaa accttcctga atgagcagcg taacgcacat
     4081 ccgaatcaga gcctgaaaaa cacactggcg gttcatctac cgaagcggtt ggttgaacgc
     4141 ttacagcaac tcgggcaaat cccgaatgtt tcgctaaaac agctcaacgt gcgtgaccaa
     4201 caggcactga ttagcacatt gaccgactgg cgtgtacaac ccaacggcac tgaaggctat
     4261 cgcactgccg aagtgacgct cggcggcgtg gacaccaacg aactctcttc acggacgatg
     4321 gaagcgcgca aagtgcctgg gctgtacttc atcggcgaag tgatggacgt taccggctgg
     4381 ctggggggct ataacttcca gtgggcgtgg tcgagtgcgt gggcttgtgc gcaggatttg
     4441 attgcagcga agtcgtcctg acctgttacc taaagatgaa aattattaga gccgcattaa
     4501 aaatgagcta attttgatag tggctatctt gtgattattt tctaatgagc ccgtgaactg
     4561 aaaccctcca ggcttaatat aaggtggaag gaaaggtgat tgaaaactca cccagtggcc
     4621 agatctttat aaaatatgaa caggctgaaa aagacagtaa ctttgttgtt tttttatcct
     4681 taaccactat gcattaatgc tgcgttattt catgatgcct aagaaaaacc agcgttacgc
     4741 aaatggtcaa cgctggtttt atccggtacg ttgcaattat tttttagcag aacccgcttc
     4801 taatattgaa acgattgaga acaacgtaaa gcataccgct cccaaaccga ccagaactcg
     4861 cgaaggaata aaatatccca tgtcggtctg cgccatttcc gcaagaaatg atgccaggaa
     4921 aaggcaaaac aggcaggtga agacgggtat catcggtatt ctgttggcta acgaacaggt
     4981 acgtctccag accagtgcca gtagccagac ttttgagaat atgctgtaac agatcattcc
     5041 gaggcaaata agaataatgc cgggagccag tcgggcgctt gcatcggaac tgactaagac
     5101 gtatatcccc tgcagtaccg tgattgagcc gagaaaaata acccaataac accacagcca
     5161 gtgttcttta gttgaaaacg tattacgtgt ttgatggaca attgtggcaa caaggccaat
     5221 tagacaggca cagattgcgg ttaacccaag caatacgtga cccgcgacat aatgcggagt
     5281 tatgtcagca ctacgtaaca gcgtaataga ccaaatgaaa ccgagcaggg tgagtaaaac
     5341 gggaacggca atgaggcaat taccgattaa tgaagaataa gcctgtaccg gcgttccatc
     5401 gctcttgctc cccgctgcat ttttgggaat gagcagaaag tgaccggatg acgctgccac
     5461 cgtcgataca caggcggcaa tcataccaac gccgaaaata acatggccgg cgacaaactc
     5521 gtctgccatc acatcattgc ctgctaacag tgcccaaccc catatcatgg tgataattga
     5581 ccccgcatag ccaataatgg ggaacaatgt attgtaaaac gtattaacgc cgcgcgtgag
     5641 ctgcgaaatg ataataaatg cggtagtgaa taatgccagg catatggccg ccagagaaat
     5701 cagcacatga cccgcgacga aatactcgct ttgccctgaa ctttccagca caaagccacc
     5761 cgatatatat gttcacttta aactcctcag gtatccctgc taataatgaa atgtgcatta
     5821 atgataattt cagcc
//
LOCUS       DAZOYL010000241         5822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZOYL010000241 DAZOYL010000000
VERSION     DAZOYL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.242"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..214
                     /locus_tag="ACHUOY_003274"
     CDS             <1..214
                     /locus_tag="ACHUOY_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase/urease nickel incorporation protein"
                     /protein_id="HFR0683974.1"
                     /translation="VRFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDA
                     QCPLCHGERLRVDTGDSLIVKSIEVE"
     gene            227..475
                     /gene="hybG"
                     /locus_tag="ACHUOY_003275"
     CDS             227..475
                     /gene="hybG"
                     /locus_tag="ACHUOY_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR0683975.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            488..569
                     /locus_tag="ACHUOY_003276"
                     /pseudo
     CDS             488..569
                     /locus_tag="ACHUOY_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(598..1464)
                     /gene="yghU"
                     /locus_tag="ACHUOY_003277"
     CDS             complement(598..1464)
                     /gene="yghU"
                     /locus_tag="ACHUOY_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR0683976.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            1669..3528
                     /gene="gss"
                     /locus_tag="ACHUOY_003278"
     CDS             1669..3528
                     /gene="gss"
                     /locus_tag="ACHUOY_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR0683977.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            3820..5319
                     /gene="pitB"
                     /locus_tag="ACHUOY_003279"
     CDS             3820..5319
                     /gene="pitB"
                     /locus_tag="ACHUOY_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR0683978.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(5368..>5822)
                     /locus_tag="ACHUOY_003280"
     CDS             complement(5368..>5822)
                     /locus_tag="ACHUOY_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HFR0683979.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
BASE COUNT         1396 a         1423 c         1554 g         1449 t
ORIGIN      
        1 cgtccgtttt agttttgaaa ttgtctgcca cggaacggtg gcgcaagggt gcgatttaca
       61 tatcgtctat aaacccgccc aggcttggtg ctgggattgc agccaggtgg tggagattca
      121 tcagcacgat gcgcagtgtc cgctctgtca cggcgagcgg ttgcgtgtcg ataccggcga
      181 ttcgctgatc gtcaaaagta ttgaagttga ataaccggag ttaataatgt gtattggcgt
      241 tccaggccag gtgctggctg tcggtgaaga tattcaccag cttgcgcagg ttgaagtatg
      301 tggtatcaag cgcgacgtga atatcgccct gatttgtgaa ggtaaccctg ccgatctact
      361 gggccagtgg gtgctggtac acgtcggatt tgccatgagc atcatcgacg aagatgaagc
      421 caaagccaca ttagacgcac tgcgccaaat ggattacgac attaccagcg cgtgatgatt
      481 agctttcgta ggcctgataa gacgcggcag cgtcgcatca ggcattgtgc acgattgccg
      541 gatgcggcgt gaacgcctta tccggcctgg atggcttgct gcgacgaaca ccaaccctta
      601 cccctgacgc ttatcttccg tattcgtctc gaaatcactg gcgtcatggc gctcatgcaa
      661 ctgctcattc agcggtccgt tggtgcggtt aacaatacgc ccacgtttca ccgccggacg
      721 ttcgcctact tctttcgccc agcgttgtac atgcttataa ctgcccgcat caagaaactc
      781 tgcggcatca tacacgccgc ctaacaccac attgccaaac cacggccaaa tcgccatatc
      841 cgcaatggtg tactcatcgc ccgcaacaaa cttatgctgc gccagttgct tatccagcac
      901 gtcgagcaga cgtttggctt ccatggtaaa gcggttgatg gcgtactcaa ttttcatcgg
      961 cgcgtaatgg taaaagtgac caaaaccacc gccgaggaac ggtgccgcgc cctgtaacca
     1021 gaacagccag ttcatcgttt cagtacgctt tgccaaatcc tgcggcagga agtagccaaa
     1081 tttctccgcc agataaagca ggatcgaacc agattcaaac acgcggatcg gcggattatg
     1141 cgtatgatcg cgcagcgccg ggatcttcga gtttgggttc acttcgacaa agccgctgga
     1201 gaattgatcg ccatcgccaa tacgaatcag ccaggcgtcg tactctgcac cagtaacgcc
     1261 cagcgccagc agctcctcaa gcataatcgt tactttctga ccgttcggcg ttcccagcga
     1321 ataaagttgc aatgggtgtt tgccaacggg cagcgttttt tcatgcgtcg gaccagaaac
     1381 cgggcgattg atattggcga acgcgccgcc agcggattta tcccacgtcc agactttcgc
     1441 gggctgataa gtattgtctg tcatagtgag ttgccttctg agtggtagtg ttgaagcagt
     1501 gtagcaggtc acttgctgta cagaatgtga attttttaac cctttgcgcc agccgctgcg
     1561 atgaggtgta tgattagtct gacctctgtc cggttaatgt cgctgagcag gtaatcagag
     1621 cacaacaatt acgccaactg cttaggttgt attgttaaag gtaaagtgat gagcaaagga
     1681 acgaccagcc aggatgcccc gttcgggaca ttattgggct acgccccagg tggggtagca
     1741 atctactctt cagattacag ttctctcgat ccgcaggaat acgaagatga cgccgtattc
     1801 cgtagctata tcgacgacga atatatgggc cacaagtggc aatgcgttga atttgctcgc
     1861 cgttttctct ttctgaatta cggtgtggtc tttactgacg tgggtatggc gtgggagatt
     1921 ttctcgctgc gcttcctgcg agaagtggtt aatgacaaca tcctgccatt gcaggcattt
     1981 cctaacggct cgccgcgtgc gccggtcgcg ggtgcgcttc ttatctggga taaaggcggt
     2041 gaatttaaag acactggcca tgtcgccatc attacccaat tgcatggcaa caaagtccgt
     2101 attgcggaac agaacgtgat tcattccccg ttgccgcaag ggcaacagtg gacgcgtgag
     2161 ctggagatgg tggtcgaaaa cggctgctat accctgaaag acacttttga tgacaccacc
     2221 attctgggct ggatgatcca gacggaagat actgaataca gcttaccgca gccggaaatt
     2281 gcaggcgagc tgctgaaaat cagcggagcg cgcctggaaa acaaaggcca gtttgacggt
     2341 aaatggctgg atgaaaaaga tccgctgcaa aacgcctatg tgcaggccaa cggtcaggtg
     2401 atcaatcagg atccttatca ttactacacc attaccgaga gtgccgagca ggagctaatt
     2461 aaagccacca acgagctgca cctgatgtat cttcacgcaa ccgacaaggt gctgaaagat
     2521 gacaacctgc tggcgctgtt cgacatcccg aaaatcctct ggccacgttt gcgtctctcc
     2581 tggcagcgtc gccgtcacca tatgatcact ggtcgtatgg atttctgcat ggatgagcgt
     2641 ggcctgaagg tttacgagta caacgccgac tccgcctcct gtcataccga agcgggcttg
     2701 atcctcgaac gttgggcgga gcagggctat aaaggcaacg gcttcaatcc ggcggaaggg
     2761 ctgattaacg aattggctgg tgcctggaaa cacagtcgtg cacgtccgtt tgtccatatc
     2821 atgcaggaca aagatatcga ggaaaactat cacgcgcagt ttatggagca ggcgctgcac
     2881 caggcgggct ttgaaacgcg tatcttgcgt ggattggatg aactgggctg ggatgctgcc
     2941 gggcaactga ttgatgggga agggcgactg gttaactgcg tgtggaaaac ctgggcgtgg
     3001 gaaaccgcgt ttgatcagat tcgtgaagtt agcgaccgtg agtttgctgc ggtgccaatc
     3061 cgtaccggtc atccgcaaaa cgaagtgcgt cttatcgacg tattgctgcg cccggaagtg
     3121 ctagtctttg agccgctgtg gacggtgatc cccggcaaca aagcgattct gccgatcctc
     3181 tggtcgctgt tcccgcacca tcgttacctg ctggataccg atttcactgt taatgatgaa
     3241 ttggtgaaaa caggttacgc agtgaaaccg attgccggtc gctgtggtag caatatcgac
     3301 ctggtcagcc atcatgaaga ggtgctggac aaaaccagcg gtaaatttgc cgagcagaaa
     3361 aacatctatc agcaactgtg gtgtttgccg aaagtggacg gtaaatacat tcaggtatgt
     3421 accttcaccg tgggcggcaa ctacggtggg acgtgtttgc gcggtgatga atcactggtc
     3481 atcaaaaaag agagtgatat tgaaccgtta attgtggtga aaaagtaata catgttgtag
     3541 caaccccgta ctgattatga agaataatca gtacggggat taatcaaaat attcaatatt
     3601 tatatgttct aatatttatt tatttcatat gtgaatatat taaccagtgg aatacctgtg
     3661 ttttgattat ttctaaaggt tttgaatgaa tgcttattgt ctgatacaca agaaataaca
     3721 ctctttttat cgttaaaaaa tgatatttca ctttgcccat gccgttaaag tcactgataa
     3781 tgcgtccgtt cgtaaattca aaatggcgta atctaatata tgctaaattt atttgttggc
     3841 cttgatatat acacagggct tttgttattg cttgctctgg catttgtgtt gttctacgaa
     3901 gcaatcaatg gttttcatga cacggcgaat gcggtggcaa ccgttattta tactcgtgcc
     3961 atgcaaccac aacttgctgt ggtgatggcg gcatttttta acttttttgg cgtgttattg
     4021 ggcggactta gcgttgccta tgccattgtc catatgttgc caaccgattt gttgctgaat
     4081 atggggtcaa cccacggcct ggcgatggtc ttttccatgc tgctggcggc gattatctgg
     4141 aacctgggaa cgtggttctt cggtttaccg gcctccagtt cgcacacctt gattggtgcg
     4201 attatcggca tcggtttaac caacgcgctg ttaaccggct catcggtgat ggatgcgtta
     4261 aacctgcgtg aagtgaccaa aattttctcc tcgctgattg tttcccctat cgtcggcctg
     4321 gtcattgcgg gaggcctgat attcctgctg cgacgctact ggagcgggac gaaaaagcgt
     4381 gaccgtattc accgcattcc ggaagatcgc aaaaagaaaa aaggcaaacg taaaccgcca
     4441 ttctggacgc gtattgcgct gattgtttcc gctgcgggcg tggcgttttc gcacggcgcg
     4501 aacgacggac aaaaagggat cggcctggtg atgctggtac tggtggggat tgcccctgct
     4561 ggcttcgtcg tcaatatgaa cgcgtccggc tatgaaatta cccgtacccg cgatgccgtc
     4621 accaacttcg aacactacct gcaacagcat cctgaactgc cgcagaagtt gattgcgatg
     4681 gaacctccat tgcctgcagc atcgactgat ggcacgcaag taacagagtt tcactgtcat
     4741 ccggcaaata cctttgatgc gattgcgcgc gttaaaacga tgctgccagg caatatggaa
     4801 agttacgagc cgttaagcgt gagtcagcgc agccagctgc gccgcattat gctgtgcatc
     4861 tctgatacct ccgcgaagct ggcgaaactg ccaggcgtca gtaaagaaga ccagaacctg
     4921 ctgaaaaaac tgcgcagcga tatgttaagc accattgagt acgctccggt gtggatcatc
     4981 atggcggtag cactggcgct cggcattggc accatgattg gctggcgtcg tgtagcgatg
     5041 accatcggtg agaagattgg taagcgcggc atgacgtatg cgcaaggcat ggcggcacaa
     5101 atgacggcgg cagtgtctat cggccttgcc agttatattg ggatgcctgt ctccacaaca
     5161 cacgtcctct cgtctgcggt agcagggacg atggtggtgg acggcggtgg gttacagcgt
     5221 aaaacggtaa ccagcatcct gatggcgtgg gtgtttactt taccggcggc aatttttctt
     5281 tctggtgggc tgtactggat agcattgcag ttgatttaac cttcctgaaa atgcccggtc
     5341 ctggcgatcg ggcatttcca tttttgacta gtgataacca cgcgcggtca taaaatccgt
     5401 aatcgctttt tctgcatcaa ccaacacctg ttccagtggc tgattggcat cgatatcaac
     5461 cagttgtgca ccaccaaagg ttaactgtgg cgttatggca atcttcctcg ccagcgattc
     5521 ccgtttatgg tcgggtttac gtgcacaggc aacttcaagg tcaacattga gtttgatgac
     5581 cagatcaggc ttatggctcg ccatccagtg aaacgcttta cgttcctggc ttgccagcca
     5641 tgagacaaaa cgaccacctt caacgttagg tgggaacacc gtaccatcgt aagcgccagg
     5701 aatttggtcc tgaggataac ggtcggttag aacaattaac ccgcgacgac gacaggcaag
     5761 catatgacga aagcgcagta agcgacgggc gacaaacgct gtaattacca gcgccggaac
     5821 tg
//
LOCUS       DAZOYL010000242         5757 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZOYL010000242 DAZOYL010000000
VERSION     DAZOYL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.243"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUOY_003281"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUOY_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR0683980.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHUOY_003282"
     tRNA            1230..1305
                     /locus_tag="ACHUOY_003282"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHUOY_003283"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHUOY_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHUOY_003284"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHUOY_003284"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR0683981.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHUOY_003285"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHUOY_003285"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR0683982.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHUOY_003286"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHUOY_003286"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR0683983.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="ACHUOY_003287"
     CDS             complement(4869..5306)
                     /locus_tag="ACHUOY_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR0683984.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         1520 a         1580 c         1273 g         1384 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgttta
//
LOCUS       DAZOYL010000243         5616 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZOYL010000243 DAZOYL010000000
VERSION     DAZOYL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.244"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHUOY_003288"
     CDS             complement(<1..513)
                     /locus_tag="ACHUOY_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0683985.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHUOY_003289"
     CDS             complement(759..1211)
                     /locus_tag="ACHUOY_003289"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0683986.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUOY_003290"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUOY_003290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR0683987.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUOY_003291"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUOY_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR0683988.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHUOY_003292"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHUOY_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUOY_003293"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUOY_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR0683989.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5616)
                     /locus_tag="ACHUOY_003294"
     CDS             complement(5494..>5616)
                     /locus_tag="ACHUOY_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0683990.1"
                     /translation="IAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1467 c         1458 g         1352 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgat
//
LOCUS       DAZOYL010000244         5576 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZOYL010000244 DAZOYL010000000
VERSION     DAZOYL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.245"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..160
                     /locus_tag="ACHUOY_003295"
     CDS             <1..160
                     /locus_tag="ACHUOY_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR0683991.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /locus_tag="ACHUOY_003296"
     CDS             complement(198..1259)
                     /locus_tag="ACHUOY_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0683992.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="ACHUOY_003297"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="ACHUOY_003297"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR0683993.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="ACHUOY_003298"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="ACHUOY_003298"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR0683994.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4154)
                     /locus_tag="ACHUOY_003299"
     CDS             complement(3453..4154)
                     /locus_tag="ACHUOY_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR0683995.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="ACHUOY_003300"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="ACHUOY_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR0683996.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
BASE COUNT         1516 a         1514 c         1409 g         1137 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag
      421 ctccagaatt ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 atcaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg
     2581 tgatttgcgc accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag
     2701 caacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccatgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt
     4321 gaagatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc
     4381 cgcgccaatg tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat
     4741 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttac
//
LOCUS       DAZOYL010000245         5552 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZOYL010000245 DAZOYL010000000
VERSION     DAZOYL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.246"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUOY_003301"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUOY_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR0683997.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHUOY_003302"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHUOY_003302"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR0683998.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUOY_003303"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUOY_003303"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR0683999.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUOY_003304"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUOY_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR0684000.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(5357..>5552)
                     /locus_tag="ACHUOY_003305"
     CDS             complement(5357..>5552)
                     /locus_tag="ACHUOY_003305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684001.1"
                     /translation="KHLFLLSLPEFSGEPRRPGGVFAVVPCQWWRIIGSYSGLTSEKY
                     KNFIVRSFFRQNILNIVFSV"
BASE COUNT         1374 a         1327 c         1509 g         1342 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgttggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tg
//
LOCUS       DAZOYL010000246         5512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZOYL010000246 DAZOYL010000000
VERSION     DAZOYL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.247"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            102..4064
                     /gene="putA"
                     /locus_tag="ACHUOY_003306"
     CDS             102..4064
                     /gene="putA"
                     /locus_tag="ACHUOY_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0684002.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(4104..4742)
                     /gene="rutR"
                     /locus_tag="ACHUOY_003307"
     CDS             complement(4104..4742)
                     /gene="rutR"
                     /locus_tag="ACHUOY_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR0684003.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            5030..>5512
                     /locus_tag="ACHUOY_003308"
     CDS             5030..>5512
                     /locus_tag="ACHUOY_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0684004.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1243 a         1494 c         1532 g         1243 t
ORIGIN      
        1 gctacgcaag tcacatttaa catggttgca caaagttgca acatcatgga tatttcacga
       61 taacgttaag ttgcaccttt cagaacaaca ggagtaatgg catgggaacc accaccatgg
      121 gggttaagct ggacgacgcg acgcgtgagc gtattaagtc tgccgcgaca cgtatcgatc
      181 gcacaccaca ctggttaatt aagcaggcga ttttttctta tctcgaacaa ctggaaaaca
      241 gcgatactct gccggagcta cctgcgctgc tttctggcgc ggccaatgag agcgatgaag
      301 caccgactcc ggcagaggaa ccacaccagc cattcctcga ctttgccgag caaatattgc
      361 cccagtcggt ttcccgcgcc gcgatcaccg cggcctatcg ccgcccggaa accgaagcgg
      421 tttctatgct gctggaacaa gcccgcctgc cgcagccagt tgctgaacag gcgcacaaac
      481 tggcgtatca actggccgat aaactgcgta atcaaaaaaa tgccagtggt cgtgcaggaa
      541 tggtccaggg gttattgcag gagttttcgc tgtcatcgca ggaaggcgtg gcgctgatgt
      601 gtctggcaga agcgttgttg cgtattcccg acaaagccac ccgcgacgcg ttaattcgcg
      661 acaaaatcag caacggtaac tggcagtcac acattggtcg tagcccgtca ctgtttgtta
      721 atgccgctac ctgggggctg ctgtttactg gcaaactggt ttccacccat aacgaagcca
      781 gcctctcccg ctcgctgaac cgcattatcg gtaaaagcgg tgaaccgctg atccgcaaag
      841 gtgtggatat ggcgatgcgc ctgatgggcg agcagttcgt cacgggtgaa accatcgcgg
      901 aagcgttagc caatgcccgc aagctggaag agaaaggttt ccgttactct tacgatatgc
      961 tgggcgaagc tgcactgact gccgcagacg cacaggcgta tatggtttcc tatcagcagg
     1021 cgattcacgc cattggtaaa gcgtctaacg gtcgtggcat ctatgaaggt ccgggcattt
     1081 caatcaaact gtcggcgctg catccgcgct acagccgcgc ccagtatgac cgggtaatgg
     1141 aagagcttta cccgcgtctg aaatcactca ccctgctggc gcgtcagtat gatattggta
     1201 ttaacatcga tgccgaagag gccgatcgtc tggagatctc cctcgatctg ctggaaaatc
     1261 tctgtttcga gccggaactg gcaggctgga acggcatcgg ttttgttatt caggcttatc
     1321 aaaaacgctg cccgttggtg atcgattacc tggttgatct cgccacccgc agccgtcgcc
     1381 gtctgatgat tcgcctggtg aaaggcgcgt actgggatag tgaaatcaag cgtgcgcaga
     1441 tggacggcct tgaaggctat ccggtttata cccgcaaggt gtataccgac gtttcttatc
     1501 tcgcctgtgc gaaaaagctg ctggcggtgc cgaatctaat ctacccgcag ttcgcgacgc
     1561 acaacgccca tacgctggca gcgatttatc aactggcagg tcagaactac tatccgggtc
     1621 agtacgagtt ccagtgcctg catggtatgg gcgagccact gtacgagcag gtcaccggga
     1681 aagttgccga cggtaaactt aaccgaccgt gtcgtattta tgccccggtt ggcacacatg
     1741 aaacgctgct ggcgtatctg gtgcgtcgcc tgctggaaaa cggtgctaac acctcgtttg
     1801 ttaaccgtat tgccgacacc tctttgccac tggatgaact ggtcgccgat ccggtcactg
     1861 ctgtagaaaa actggcgcaa caggaagggc aaactggatt accgcatccg aaaattcccc
     1921 tgccgcgcga tctttacggt cacgggcgcg acaactcggc agggctggat ctcgctaacg
     1981 aacaccgtct ggcctcgctc tcctctgccc tgctcaatag tgcactgcaa aaatggcagg
     2041 ccttgccaat gctggaacaa ccggtagcgg caggtgagat gtcgcccgtt attaaccctg
     2101 cggaaccgaa agatattgtg ggctttgtgc gtgaagccac gccacgtgaa gtagaacagg
     2161 cgctggaaag tgcggttaat aacgcgccaa tctggtttgc cacgcctccg gctgaacgcg
     2221 cggcgatttt gcatcgcgct gccgtgctaa tggaaagcca gatgcagcaa ctgattggta
     2281 ttctggtgcg tgaagccgga aaaaccttca gtaacgccat tgccgaagtg cgcgaagcgg
     2341 tcgattttct ccactactac gccggacagg tgcgggatga tttcgctaac gaaacccacc
     2401 gtccattagg gcctgtggtg tgtatcagtc cgtggaactt cccgctggct attttcaccg
     2461 ggcagatcgc tgccgcactg gcggcaggta acagcgtgct ggcaaaaccg gcagaacaaa
     2521 cgccgctgat tgccgcgcaa gggatcgcca ttttgctgga agcgggtgta ccccctggcg
     2581 tggtgcaatt gctgccaggt cagggggaaa ccgtgggcgc gcaactgacg ggtgatgatc
     2641 gcgtgcgcgg ggtgatgttt accggttcaa ccgaagtcgc tacgttactg cagcgcaata
     2701 tcgccagccg cctggacgct cagggtcgcc ctattccgct catcgctgaa accggcggca
     2761 tgaacgcgat gattgtcgat tcttcagcac tgaccgaaca agtcgtcgtg gatgtgctgg
     2821 cctcggcgtt cgacagtgcg ggtcagcgtt gttcggcact gcgcgtgctg tgcctgcaag
     2881 atgagattgc cgaccacacg ttgaaaatgc tgcgcggtgc aatggccgaa tgccggatgg
     2941 gtaatccggg tcgcctgacc accgatatcg gtccggtgat tgatagcgaa gcgaaagcca
     3001 atattgagcg ccatattcag accatgcgta gcaaaggccg tccggtgttc caggcggtgc
     3061 gggaaaacag cgaagatgcc cgtgaatggc aaagcggcac ctttgtcgcc ccgacgctga
     3121 tcgaactgga tgactttgcc gaattgcaca aagaggtctt tggtccggtg ctgcatgtgg
     3181 tgcgttacaa ccgtaaccag ctaccagagc tgatcgagca gattaacgct tccggctatg
     3241 gtctgacgct tggtgtccat acgcgtattg atgaaaccat cgcccaggtc actggctcgg
     3301 cccatgttgg taacctgtat gttaaccgta atatggtggg cgcagtggtt ggtgtgcagc
     3361 cgttcggcgg cgaagggttg tccggtaccg ggccgaaagc aggcggtccg ctctatctct
     3421 accgtctgct ggcgaatcgc ccggaaagtg cgctggcagt gacgctcgcg cgtcaggatg
     3481 cagagtatcc ggtcgatgca cagttgaaag ccgcattgac tcagccgcta aatgcactgc
     3541 gggaatgggc agcaaatcgt ccagaattgc aggcgttatg tacgcaatat ggcgagctgg
     3601 cgcaggcagg aacacaacga ttgctgccgg ggccgacggg tgaacgcaac acctggacgc
     3661 tgctgccgcg tgagcgcgtg ttgtgtattg ccgatgatga gcaggatgcg ctgactcagc
     3721 tcgccgccgt gctggcggtg ggcagccagg tactgtggcc ggatgacgcg ctgcatcgtc
     3781 agctagtaaa ggcattgcca tcggcagtca gcgaacgtat tcaactggcg aaagcggaaa
     3841 atataaccgc tcaacccttt gatgcggtga tcttccacgg tgattcggat cagcttcgcg
     3901 cattgtgtga agccgttgct gcgcgggatg gcgcaattgt ttcggtgcag ggttttgccc
     3961 gtggcgaaag caatatcctt ctggaacggc tgtatatcga acgttcgctg agtgtgaata
     4021 ccgctgccgc tggcggtaac gccagtttaa tgacgatcgg ttaaacaggt agccggagga
     4081 tgttacaacc tcctccggca tctttaacgt ggtcgaatcc cctcaataat aatccgctgc
     4141 acgttttcaa ccgtttgatt gaaaaatatc tcatcgcgca acgtcgcgcc tgtcaccgcc
     4201 tccacctgag gggcgaaatc ggcgtaatgt tgagtggaag cccaaatcat gaaaatcaaa
     4261 tgctgcggat caatcggcgc gagtttgccg cttttgaccc aaccggcaat cagcgcagat
     4321 ttctcatcaa ttaatgcctt caaatcgccc gtcagttcat ccattaacag cggcgcgcct
     4381 gccagcatct ccatacagaa caggcgcgaa gcctgcggat aatcgcggga gacttccagc
     4441 ttcagacgga tgtactcttt tatcgccgcc agcggagcga aatcttcacg aaacgctttt
     4501 aacggtgcca gccagatatc gagaatctgc cgcagcacgg caatatacag cgcctctttt
     4561 gacggaaagt aatacagcag attggttttt gaaacacccg ccaactctgc gatctgctcc
     4621 agccttgtgc cgtgaaaacc gaattgtgaa aaagtgtcca gtgctgcgct aagaatcgct
     4681 tttttcttcg cgcttactgc gcgcgaacgt ttacccgttg ttttcactgc gccttgcgtc
     4741 atgcgctctc ccctcttgta gattggcaac aggatagcaa aacccccgcc cggcctcgac
     4801 cttctgcact ctcatcgcgc acagatgcat gttttatgtg caactgtttt gaccgtttag
     4861 tccacttttt atcagatatt ttagataacg taatattaac atattaataa cattaacttt
     4921 ttaaaaactg gcatccgctt tgcaaacaag ccaatacgcg gctgaaaagg atatgcagga
     4981 tgcagcgccc cgtctgactt tcactttacg agacgaagag aggttgatga tgaaaattgg
     5041 cgtattcgta cctattggca acaacggctg gctcatttcg acccacgcgc cgcagtacat
     5101 gccgaccttt gaactgaata aagccattgt gcagaaagcg gagcattacc atttcgattt
     5161 cgccctgtcg atgatcaaac tgcgtggctt tggcggcaaa actgagttct gggatcacaa
     5221 ccttgagtcg ttcaccttga tggcgggtct ggcagccgtg acctcgcgca ttcagattta
     5281 cgccactgct gccaccttaa cgttacctcc ggcaatcgtc gcccgtatgg ccgcaaccat
     5341 cgactccatc tctggcgggc gttttggcgt caacctcgtg actggctggc aaaagcccgt
     5401 gtatgagcag atgggtatct ggcctggcga tgactatttc tcccgtcgtt acgactatct
     5461 caccgaatat gttcaggtgc tgcgcgacct gtggggcacg gggaaaagcg at
//
LOCUS       DAZOYL010000247         5494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZOYL010000247 DAZOYL010000000
VERSION     DAZOYL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.248"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..115)
                     /locus_tag="ACHUOY_003309"
     CDS             complement(<1..115)
                     /locus_tag="ACHUOY_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0684005.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="ACHUOY_003310"
     CDS             complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="ACHUOY_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR0684006.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2333..2968)
                     /gene="nth"
                     /locus_tag="ACHUOY_003311"
     CDS             complement(2333..2968)
                     /gene="nth"
                     /locus_tag="ACHUOY_003311"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR0684007.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="ACHUOY_003312"
     CDS             complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="ACHUOY_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR0684008.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="ACHUOY_003313"
     CDS             complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="ACHUOY_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR0684009.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="ACHUOY_003314"
     CDS             complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="ACHUOY_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR0684010.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5350..>5494)
                     /locus_tag="ACHUOY_003315"
     CDS             complement(5350..>5494)
                     /locus_tag="ACHUOY_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HFR0684011.1"
                     /translation="AKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVV
                     NED"
BASE COUNT         1447 a         1393 c         1349 g         1305 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 acgctgcgga acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc
      541 gatgctctgc aggccatagc ttgcgaccag ccagcttaca gacacgatac cagcgtcaga
      601 cgcgaatttc gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat
      661 tgcaaacttg gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat
      721 cggactaccg atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc
      781 cagacccaga atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat
      841 ctggctgtac agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat
      901 tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc
      961 gaaggcaaca acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc
     1021 gatagcgatc agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg
     1081 ctcgaagtct ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac
     1141 gatagtgatc agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc
     1201 ggccagccac ggcgtagcaa tcatagagaa gaaagagccg atgttgacgg acatgtagta
     1261 catggtgaat gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga
     1321 agatgggtta gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac
     1381 gataccggcg tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc
     1441 accgagcata attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc
     1501 gaccagacca taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga
     1561 catacccagt tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa
     1621 acgttcccat aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt
     1681 caaactgacg ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac
     1741 atcccatatt aacgggggtg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt
     1801 atagtgggag gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt
     1861 gtaataatct gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc
     1921 agcgttaaat ttttcaaaaa acgtaatggc agattttttc actgcactga gaggggggtg
     1981 atggcttaac ggcgatatgt tctgctattt agcgttttta ctagtgagat agtccagttt
     2041 ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt gttgcgtgtg taaatttaaa
     2101 ttattttaac ctgattcttg caatgttttt acattgcgac agcatcgtga tcttgatcac
     2161 gaatgaatag aaaatttcgc tattaaaaaa acaattaacc tgcatttttg gttatccgca
     2221 aacggattac ctgatgataa cgctaagcaa tggcatcatt cacgttaaaa agatgtttta
     2281 aaagaggata aagaaaggtt atcaatgggg taatcggtgt tacccctttt cttcagatgt
     2341 caactttctc tttgtattca caaagatctt caataataca agagccacag cggggcttgc
     2401 gggcaatgca ggtataacgc ccgtgcagga tcaaccaatg gtggcagtcg actttaaact
     2461 ctgccggaac cactttcagt agcttttctt ctacctgttc gacgtttttc cccggcgcaa
     2521 attgagtacg attacaaacg cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga
     2581 atgcagtgtt taatacgacg ttggctgttt tacgacctac gccgggcagg gcttcaagcg
     2641 cagcacgatc ttccggaacc tcgccattat gctgctccag taagatacgg caggttttga
     2701 tgatattttc tgctttgctg ttataaagcc caatcgtttt gatataggtt ttaacccctt
     2761 caacgcccag ttcaagcatc gctgcaggcg tattcgccac cgggtaaagt ttcgccgtcg
     2821 ccttattaac actgacatcg gtcgcctgag cggaaagcag tacggcaatc agcaattcaa
     2881 aaggcgaact gaaattaagc tcggtggtgg gatgaggatt gttctcacgc aggcgagtga
     2941 ggatctccag gcgttttgct ttgttcatca gacattccct gtttcaccgt ttggcaatgc
     3001 acgttcggca gctgcttcag tacggcgctt tttcattctt tcatcaatca ggtattttcc
     3061 tgccagcatc agtcccaggc caataaatgc acctggtggc agcatcgcca gcaggaaagg
     3121 ggagtcggtg tggaaaatct ccacgcgtaa tacttttgcc cagctaccta acagcgcatc
     3181 tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt agtgaaccca gcacgaacat
     3241 ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt gccgaaagcg ccggaccttt
     3301 tttggcggcg aaggcttcag cgcggcccac aacgatacag ttagtgacaa tcagcggaat
     3361 aaaaatccct aatgattgat acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc
     3421 gctgaccacc gaggcgatga tcatcacgta aatgggaatg cggatctcgg ctggcgtcca
     3481 gtgacgcagc gtcgaaatgg tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc
     3541 taaacccaga gcgttagtgg cagtggacgt gaccgccaac agaggacaaa ggccgagcaa
     3601 ctggaccagc gcagagttgt ttttccacaa cccctgaaca ataacgtctt taatttcgct
     3661 cacgctttat tctccacagg caggaagttg agaaagttgt gccggtaacg tctgagcgta
     3721 caatccggcg cgttttaccg cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa
     3781 ctggtcgaaa tcaccaccat ctttcttcac cgcccagtgc gcatcatccg caccactgat
     3841 ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc agttcgattt tatcgccaag
     3901 ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt accgtgccgt taaaatcggc
     3961 tcccaccagc agctgaatcg caccggaata gccatctggc gcggttgctt ccagaacggc
     4021 ggctaccggt ttgtcatcct gtttggcgat gtaaacccga tgctcacctt tacctaactc
     4081 tggcgcagtt accagatagc aactctgtgc cagcgtattg ttatagcgtt cggctggcag
     4141 cacctgatca aataatgcct tttgttgcag actggcctgt tcagcaatcg tcgttttggt
     4201 catctggttg atggccgcag ttaaccctgt tgaacccgct gcaaatagcg ctaacgtaat
     4261 gccgtgtttt cggatagttt tcagcatggt ttaccctttg cgatggccgt agacgcgcgg
     4321 acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc agcaggacgg caaaagccac
     4381 gccgtcagga tagccgccga aactgcggat caaccagact aataagcccg caagcgcgcc
     4441 gaaaataaga cgaccacgat tggtcgtaga agcggtaacc gggtcagtca aaataaagaa
     4501 tgcgccgagc atggtcgcac cagacagcag atgaatttgc ggtgctgcca gtgtttctgg
     4561 tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt actaagaagc tgaggggaat
     4621 atgccagcga atcgctttct gccatagcaa ccacaggccg ccagccagcc aggcgagatt
     4681 tacccattgc caaccagcgc ccgccagaat accgctgtag atcggatatt gcataatctg
     4741 ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta tccagcggtg tcgcctgact
     4801 aatgccatca atacctaagc gtagtgtgtt catatcacca ccactggcgg tatgcccgct
     4861 gaaaataacc tgtatggcgt cgataaaacc agggatgttg accgcaattt catgtggcgg
     4921 taaccagctg gtcatctgca ccgggaagga gatcagtaag accacataac caatcattgc
     4981 cggattaaac gggttttgcc ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa
     5041 caccgtaccc agcacgacca tccaccatgg cgcgaggggg ggaatactta ccgccagcaa
     5101 taagcctgtc agcaatgctg agttatcttt caacgttgcg gctaccgact gcttgcgtag
     5161 tttgagtacg agagcttcgg ctaacagagt actaaccgat gccaacagga tctgaacgag
     5221 agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct ggcacggctg cgagcaacac
     5281 caacagcata atgcgcgatg tctggcgctg gttatgggta taaggggagc tagctattct
     5341 gaataccatt tagtcctcgt ttacaacctt ctgctgggcg gcttttttgg cctgagcgcg
     5401 ggcaatagcc gcggcaactg ccgctttgcg cggatcaacc tgttcttctg gttctgcgtt
     5461 agtctgttgc tgttccagct tgcgcgcttt ggca
//
LOCUS       DAZOYL010000248         5489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZOYL010000248 DAZOYL010000000
VERSION     DAZOYL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.249"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(202..297)
                     /locus_tag="ACHUOY_003316"
                     /pseudo
     CDS             complement(202..297)
                     /locus_tag="ACHUOY_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(294..860)
                     /locus_tag="ACHUOY_003317"
     CDS             complement(294..860)
                     /locus_tag="ACHUOY_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0684012.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            1170..2942
                     /gene="aspS"
                     /locus_tag="ACHUOY_003318"
     CDS             1170..2942
                     /gene="aspS"
                     /locus_tag="ACHUOY_003318"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR0684013.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            3060..3512
                     /gene="nudB"
                     /locus_tag="ACHUOY_003319"
     CDS             3060..3512
                     /gene="nudB"
                     /locus_tag="ACHUOY_003319"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR0684014.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            3541..4281
                     /locus_tag="ACHUOY_003320"
     CDS             3541..4281
                     /locus_tag="ACHUOY_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0684015.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            4316..4837
                     /gene="ruvC"
                     /locus_tag="ACHUOY_003321"
     CDS             4316..4837
                     /gene="ruvC"
                     /locus_tag="ACHUOY_003321"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR0684016.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4839..5441)
                     /locus_tag="ACHUOY_003322"
     CDS             complement(4839..5441)
                     /locus_tag="ACHUOY_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR0684017.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1387 a         1323 c         1486 g         1293 t
ORIGIN      
        1 tcaagagtga gggaaagtga ttttttgggg catgcatggg gcaaaaatat ttgttgtggg
       61 gcattttggg gcatgattgg gacactttgt tacatatgaa ctttgccgat tttcatatgg
      121 catggcaatc caacgctttg attattctaa agaaaacaca tgctcttggg cgttctttag
      181 tgatttttaa aaatcccgta tcacgcagtt aaagtggcgg gcatactctt caaggctggt
      241 gatccccaac cgcacccatt taggatgcga ccattgcggt agaccgatgt aaatcataac
      301 gcgtgaagga tctcttccac gctacgcaca cgggcgatgc gcgggtagat atgattaatg
      361 ctgttattgt gttgctcggc gctggcggca ctacaggcgt cttcggcaat caccagatta
      421 aagccaagtt cccaggcatt gcgggcggtg gattcaacac cgatattggt cgagatccca
      481 cataacacta ttgtatcgat acctcggcgg cgcagttgca actccagatc cgtaccgtaa
      541 aacgcacccc actgacgttt gatgatttcg atatcactgt cagttgcacc taatgcagct
      601 ggatgttgcc accagttttc gggcaacact ttagcggggg agggggcatc aaccggctgt
      661 tttaatgctt cggcgtaatc ggcagaccag ccaactcgca ccagaaacac gggctgaccg
      721 ctggcgcgaa atttcgccgc cagcttcccg gcgcgattaa ctacctcatc ggcagtatgt
      781 ggaccaccgg caaaaggtaa gatgccttct tgtaaatcga tcaccaccaa cgcagtggtt
      841 ttagcattaa gttcaagcat gctgactcct gaaaaaacaa aatcgtgcct cacaccttaa
      901 tgcgattagc cgatggcgga acgacaaatt ttgttaattt ttatgagttc gggcaacacc
      961 tttatagcaa actggcgtag agtctgattt caggcccgat ataaagtggt aacgaggcgt
     1021 tacagcgcgg gccaggctct tacggtgcgg cagaatttcc agtataatag ccgccttttt
     1081 tcattcagtt gtgacataca gctaacgctg cgacttggtc acctgcgcag cagggggcat
     1141 ccgccggcag ctaagttaag ggatatctca tgcgtacaga atattgtgga cagctccgtt
     1201 tgtcccacgt ggggcagcag gtgactctgt gtggttgggt caaccgtcgt cgtgatcttg
     1261 gtagcctgat cttcatcgat atgcgcgacc gcgaaggtat cgtgcaggta tttttcgatc
     1321 cggatcgtgc ggacgcgtta aagctggcct ctgaactgcg taatgagttc tgcattcagg
     1381 ttacgggcac cgtacgtgcg cgtgacgaaa aaaatattaa ccgcgatatg gcgaccggcg
     1441 aaattgaagt gctggcgtcc tcgctgacta tcatcaaccg cgcagatgtt ctgccgcttg
     1501 actctaacca cgtcaacacc gaagaagcgc gtctgaaata ccgctacctc gacctgcgtc
     1561 gcccggaaat ggcgcagcgc ctgaaaaccc gcgctaaaat caccagcctg gtgcgccgtt
     1621 ttatggatga ccacggcttc ctcgacatcg aaactccgat gctgaccaaa gccacgccgg
     1681 aaggcgcgcg tgactacttg gtgccttctc gtgtgcacaa aggtaaattc tacgcactgc
     1741 cgcaatcccc acagttgttc aaacagctgc tgatgatgtc cggttttgac cgttactatc
     1801 agatcgttaa atgcttccgt gacgaagacc tgcgcgctga tcgtcagcct gaatttactc
     1861 agatcgatgt ggaaacttct ttcatgaccg cgccgcaagt gcgtgaagtg atggaagcgc
     1921 tggtgcgtca tttgtggctg gaagtgaagg gcgtggatct gggcgatttc ccggtaatga
     1981 cctttgcgga agcagaacgc cgttatggtt ctgataaacc ggatctgcgt aacccgatgg
     2041 aactgaccga cgttgccgat ctgctgaaat ctgttgagtt tgcagtattt gcggggccgg
     2101 cgaacgatcc gaaaggccgt gttgccgctc tacgtgttcc gggtggcgca tcgctgaccc
     2161 gtaagcagat cgacgaatac ggtaacttcg ttaaaatcta cggcgcgaaa ggtctggctt
     2221 acatcaaagt taacgaacgc gcgaaaggtc tggaaggtat caacagcccg gtagcgaagt
     2281 tccttaatgc agaaatcatc gaagccatcc tggatcgtac tgccgcgcaa gatggcgata
     2341 tgattttctt cggtgccgac aacaagaaaa ttgttgccga cgcgatgggc gcactgcgcc
     2401 tgaaagtggg taaagacctt ggtctgaccg acgaaagcaa atgggcaccg ctgtgggtta
     2461 tcgacttccc gatgtttgaa gacgacggtg aaggcggcct gacggcaatg caccatccgt
     2521 tcacctcacc gaaagacatg actgctgcag aactgaaagc tgcaccggaa aatgcggtgg
     2581 cgaacgctta cgatatggtc atcaatggtt acgaagtggg cggtggttca gtacgtatcc
     2641 ataatggtga tatgcagcag acggtgtttg gtattctggg tatcaacgaa gaggaacagc
     2701 gcgagaaatt cggcttcctg ctcgacgccc tgaaatacgg tactccgccg cacgcaggtc
     2761 tggcattcgg tcttgaccgt ctgaccatgc tgctgactgg caccgacaat atccgtgacg
     2821 ttatcgcctt cccgaaaacc acggcggcag cgtgtctgat gactgaagca ccgagctttg
     2881 ctaacccgac tgtactggct gagctgagca ttcaggttgt gaagaaggct gagaataact
     2941 gatatgactc aaatacacga aatcattcgc gttgcatcaa ggcggcaact gagtgaactc
     3001 ccatgagcat agataactat gtgaatggga tgagcgaagg cagtcaacga agaggcagcg
     3061 tgaaggataa agtgtataag cgtcccgttt cgatcttagt ggtcatctat gcacaagata
     3121 cgaaacgggt gctgatgttg cagcggcgtg acgatcccga tttctggcag tcggtaaccg
     3181 gcagcgtgga agagggtgaa accgcgccgc aagctgccat gcgcgaagta aaggaagagg
     3241 tcaccattga tgttgtcgct gaacaactga ccttaattga ctgtcagcgc acggtagagt
     3301 ttgaaatttt ttcacattta cgtcatcgct atgcgccggg cgtgacgcgt aatacggaat
     3361 catggttctg tcttgcgctc ccgcacgaac gccaggtagt cttcactgaa catctggctt
     3421 acaagtggct tgatgcgcct gctgccgcgg cgctcactaa gtcctggagc aaccggcagg
     3481 cgattgaaca gtttgtaatt aacgctgcct gaacaggcag ggctatctgg agatattttt
     3541 atggcaggtc atagtaaatg ggccaacacc agacatcgta aagctgcgca ggatgctaag
     3601 cgcggtaaaa tcttcactaa aatcattcgt gagctggtaa ccgcggctaa gctgggcggt
     3661 ggcgatccgg acgctaaccc gcgtctgcgt gcggcggtgg ataaagcact gtctaacaac
     3721 atgacccgtg acacactgaa ccgcgcaatt gcacgtggtg tgggcggtga tgatgatgca
     3781 aacatggaaa ccatcatcta cgaaggttac ggtcctggcg gcacggcaat catgattgaa
     3841 tgtctgtctg acaaccgcaa ccgtaccgtt gctgaagtgc gtcatgcatt tagcaaatgt
     3901 ggcggtaacc tcggtactga tggttccgtt gcctatctgt tcagcaaaaa aggcgtgatc
     3961 tccttcgaga aaggcgatga agacaccatt atggaagcag cactggaagc aggtgctgaa
     4021 gacgttgtga cctatgatga cggcgcgatt gacgtctaca ctgcatggga agaaatgggt
     4081 aaagtgcgcg acgctctgga agcggcaggt ctgaaagcag acagcgcgga agtttccatg
     4141 atcccgtcca ccaaagctga tatggatgca gaaaccgcac cgaaactgat gcgtctgatc
     4201 gatatgctgg aagattgcga cgacgtgcag gaagtctacc ataacggtga aatctctgat
     4261 gaggttgcag caactctctg atggggcctg ctaaacagca aaacggagac gcgtgatggc
     4321 tattattctc ggcattgacc cgggttcgcg cgtgatcggc tatggtgtca tccgccaggt
     4381 aggcaggcaa ctgtcctacc tgggtagcgg atgcatacgc accaaagtgg atgatttacc
     4441 gtctcgtctg aagctcatct atgcgggcgt gacggaaatc atcacccagt tccagccaga
     4501 ttatttcgcc attgaacaag tctttatggc caaaaatgcc gactcggctc tgaaactggg
     4561 gcaggcgcgc ggcgtggcga ttgtggcggc ggtgaatcag gaattgccgg tatttgaata
     4621 cgcggcacgt caggtaaagc aaacggtggt aggtattggt agtgccgaaa aaagccaggt
     4681 gcagcatatg gtccgcacct tgctgaaact gcccgctaat ccacaggcgg atgccgccga
     4741 tgcgttagct atcgcgatta cccattgtca cgtcagccag aatgcaatgc agatgagcga
     4801 atcgcggctg aacctggcga gagggcgact gcgttaagtt ataccgcctc ggtcagttca
     4861 ggctgaggcg tttcaactcc ctccgcgtga atcaacacca gacccggatg atgccacaaa
     4921 ctggctggcg tgacggtttt acgctcccac ggaatagaac ctaagaacca gaatttccag
     4981 aaagtgagtt ttgcattcgg attgctattt aacagttctc caaacgtttc tatttcaccc
     5041 accggaatgc atagcgcttc tttataaata tcaaaaacaa attttgaaca gaactggcgc
     5101 gaagattcgt atttaaaacc ggtgtggtaa agtttgcgta gccgggaggg aacctgttca
     5161 acaattcgtt gtttttgttg ttctgtcagc ccagcgtcta atcgctttat agcatagcgt
     5221 tgattagacg aacgtttaat aaaacgagat agcgtggtga tggttgaaag gggaacccgg
     5281 ctttctgcaa ccaggaagtc ttcaccgtta tgaccgataa tgatcccgac gtgattactc
     5341 cagcaatttg atgcagctga tatttgacca aataaggcag cacctataca tgtaaagacg
     5401 atatctccaa tttcatattc agcagggtaa ttaatattca cgttgtcatt ccattgaaat
     5461 agatacgaat atacgtaata gcataaaaa
//
LOCUS       DAZOYL010000249         5482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZOYL010000249 DAZOYL010000000
VERSION     DAZOYL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.250"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(281..424)
                     /gene="ypaB"
                     /locus_tag="ACHUOY_003323"
     CDS             complement(281..424)
                     /gene="ypaB"
                     /locus_tag="ACHUOY_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR0684018.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            552..2837
                     /gene="nrdA"
                     /locus_tag="ACHUOY_003324"
     CDS             552..2837
                     /gene="nrdA"
                     /locus_tag="ACHUOY_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0684019.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3071..4201
                     /gene="nrdB"
                     /locus_tag="ACHUOY_003325"
     CDS             3071..4201
                     /gene="nrdB"
                     /locus_tag="ACHUOY_003325"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0684020.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4201..4455
                     /gene="yfaE"
                     /locus_tag="ACHUOY_003326"
     CDS             4201..4455
                     /gene="yfaE"
                     /locus_tag="ACHUOY_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR0684021.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4509..5158)
                     /gene="inaA"
                     /locus_tag="ACHUOY_003327"
                     /pseudo
     CDS             complement(4509..5158)
                     /gene="inaA"
                     /locus_tag="ACHUOY_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(5238..5342)
                     /locus_tag="ACHUOY_003328"
                     /pseudo
     CDS             complement(5238..5342)
                     /locus_tag="ACHUOY_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5337..5482
                     /locus_tag="ACHUOY_003329"
                     /pseudo
     CDS             5337..5482
                     /locus_tag="ACHUOY_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1389 a         1447 c         1317 g         1329 t
ORIGIN      
        1 agttaataac cttaagggat accagttata tttaaactaa attatagtaa tgaataattt
       61 tcttataata taaggtaaat taacaaaatg gcttcgcatt taacaataac cgaatagaaa
      121 acaaccattt cgacatcaac aatcgcttac attcgcttat atattgacca caactgatac
      181 atcagattat gtgatgactc gtgcttagat caatttttgc aatcattaac aaaaagatta
      241 ataaaccatc tatatcaatt tatccaacct attctgccgt tcaagaaatc gccgaacagt
      301 tatttttaac aaatttttct cttcccattg actttcccgg acaccttgtc tgacctaagg
      361 tgcgcgaaag ccactttttc cttcctgagt tatccacaaa gttatgcact tgcaagaggg
      421 tcattttcac actatcttgc agtgaatccc aaacataccc cctatatata gtgttctaag
      481 cagcttccct tactacaggt agtctgcatg aaactattgc ggaaagaatt ccaaaaacag
      541 gtacgacata catgaatcag aatctgctgg tgacaaagcg cgacggtagc acagagcgca
      601 tcaatctcga caaaatccat cgcgttctgg attgggcggc agaaggactg cataacgttt
      661 cgatttccca ggtcgagctg cgctcccaca ttcagtttta tgacggtatc aagacctctg
      721 acatccacga aaccattatc aaggctgccg cagacctgat ctcccgtgat gcgccggatt
      781 atcagtatct cgccgcgcgc ctggcgatct tccacctgcg taaaaaagcc tacggccagt
      841 ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt cgagatgggc aaatacgata
      901 atcatctgct ggaagactac acggaagaag agttcaagca gatggacacg tttatcgatc
      961 acgaccgtga tatgaccttc tcttatgctg ccgttaagca gctggaaggc aaatatctgg
     1021 tacagaaccg cgtgaccggc gaaatctatg agagcgccca gttcctttat attctggttg
     1081 ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct gcaatatgtg aagcgttttt
     1141 acgacgcggt ttccacattt aaaatttcgc tgccgacgcc aatcatgtcc ggcgtgcgta
     1201 ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg cggtgacagc ctggattcca
     1261 tcaacgccac ctccagcgcg attgttaaat acgtttccca gcgtgccggg atcggcatca
     1321 acgccgggcg tattcgtgcg ctgggtagcc cgattcgcgg tggtgaagcg ttccataccg
     1381 gctgcattcc gttctacaaa catttccaga cagcggtgaa atcctgctct cagggcggtg
     1441 tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca tctggaagtg gaaagcctgc
     1501 tggtgttgaa aaacaaccgt ggtgtggaag gcaaccgcgt gcgtcatatg gactacgggg
     1561 tacaaatcaa caaactgatg tatacccgtc tgctgaaagg tgaagatatc accctgttca
     1621 gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc cgatcaggaa gagtttgaac
     1681 gtctgtatac caaatatgag aaagacgaca gcatccgcaa gcagcgtgtg aaagccgttg
     1741 agctgttctc gctgatgatg caggaacgtg cgtctaccgg tcgtatctat attcagaacg
     1801 ttgaccactg caatacccat agcccgtttg atccggccat cgcgccagtg cgtcagtcta
     1861 acctgtgcct ggagatagcc ctgccgacca aaccgctgaa cgacgtcaac gacgagaacg
     1921 gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg cgcaattaat aacctggatg
     1981 aactggaaga gctggcaatt ctggcggttc gtgcacttga cgcgctgctg gattatcagg
     2041 attacccgat cccggccgcc aaacgtggag cgatgggtcg tcgtacgctg ggtattggtg
     2101 tgatcaactt cgcttactac ctggcgaagc acggtaaacg ctactccgac ggcagcgcca
     2161 acaacctgac gcataaaacc ttcgaagcca ttcagtatta cctgctgaaa gcctctaatg
     2221 agctggcgaa agagcaaggc gcgtgcccgt ggtttaacga aaccacttac gcgaaaggga
     2281 tcctgccgat cgatacctat aagaaagatc tggataccat cgctaatgag ccgctgcatt
     2341 acgactggga agcattgcgt gaatcaatca aaacgcacgg cctgcgtaac tctacgcttt
     2401 ctgccctgat gccgtccgag acctcttcgc agatctctaa cgccactaac ggtattgaac
     2461 cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg tattttgcgc caggtggtgc
     2521 cggactacga gcacctgcac gacgcctatg agctgctgtg ggaaatgccg ggtaacgatg
     2581 gctacctgca actggtaggt atcatgcaga aatttatcga tcagtcgatc tctgctaaca
     2641 ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc gatgcagcag ttgctgaaag
     2701 acctgctcac cgcctacaaa ttcggggtca aaacgctgta ttatcagaac acccgtgacg
     2761 gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca ggacgatggc tgcgaaagcg
     2821 gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt aaacgcctta tccggcctac
     2881 ggctcggttt gtaggcctga taagacgcgc cagcgtcgca tcaggctccg ggtgccggat
     2941 gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt aggcctgata agacgcgcca
     3001 gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat ccggcattaa actcccaaca
     3061 ggacacactc atggcatata ccaccttttc acagacgaaa aatgatcagc tcaaagaacc
     3121 gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat cagcaaaaat atgacatctt
     3181 cgaaaagctg atcgaaaagc agctctcttt cttctggcgt ccggaagaag ttgacgtctc
     3241 ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa aaacacatct ttatcagcaa
     3301 cctgaaatat cagacgctgc tggattccat tcagggtcgt agcccgaacg tggcgctatt
     3361 gccgcttatt tctattccgg aactggaaac ctgggtcgaa acctgggcgt tctcagaaac
     3421 gattcattcc cgttcctata ctcatatcat tcgtaatatc gttaacgatc cgtctgttgt
     3481 gtttgacgat atcgtcacca acgagcagat ccagaaacgt gcggaaggga tctccagcta
     3541 ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt ctgggcgaag gcacccacac
     3601 cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg aagaaaaaac tgtatctctg
     3661 cctgatgagc gttaacgcgc tggaagcgat ccgcttctac gtcagctttg cttgttcctt
     3721 tgcctttgca gaacgcgaac tgatggaagg taacgccaag attattcgcc tgattgcccg
     3781 cgatgaagcc ctgcacctga ccggtactca gcatatgctg aatctgctgc gcagcggcgc
     3841 ggatgatccg gagatggcgg aaattgccga agaatgtaag caggagtgct atgacctgtt
     3901 tgtccaggcg gcacagcaag agaaagactg ggcggattat ctgttccgtg acggttcgat
     3961 gattggtctg aataaagata ttctctgcca gtacgttgaa tacatcacca atatccgcat
     4021 gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc aacccgatcc cgtggatcaa
     4081 cacctggctg gtgtctgata acgtgcaggt tgctccgcag gaagtggaag tcagttctta
     4141 tctggtcggg cagattgacg cggaagtgga caccgacgat ttgagtaact tccagctctg
     4201 atggcccgcg ttaccctgcg catcactggc acacaactgc tgtgccagga tgaacaccct
     4261 tcccttctgg cggcgctgga atcccacaat gttgcggttg agtaccagtg ccgcgaaggt
     4321 tactgcggct cctgtcgcac ccgactggtc gcaggtcagg tggactggat tgccgaaccg
     4381 ttagccttta ttcagccggg ggaaattttg ccctgttgtt gccgggcaaa aggcgatatt
     4441 gaaatcgaga tgtgaattgg ttgtagtgcc agatacaacg cttatacgtc ttatctggcc
     4501 tacaacgatt acatggcgta gtaatacgct ttcacctgct cccagtccgc tttggggatt
     4561 ggctccagat attttccagc tggcggaagt catgattaat cgctttattg cgacgcaagc
     4621 ggcgacgact tttctccagg tcaagaaaac cagcttctgc tttaccttct gttttcacat
     4681 agatatggcg aacataacaa cagccatgct gacgattaac gctatgcatt ttcttaaacg
     4741 ccagcgccac tgctttcaac atggcttgcc gtacttcgtc agaatagggc gatactgcat
     4801 gccgggcata ccagtcagca atgctgatga accccgccat atcttcagtc accagcaacg
     4861 cgcgccattc accctcaatt ttcaccgctt caccaaaaac gatcttcggc acgatgacac
     4921 cggcccgttc cagttcttta attaccgcaa cctcacggac aatcgttggt cggccgaacg
     4981 gataacgtac ggaatgaaac agatgatgcg tcatgcgctt tacatacagc tttttgccgt
     5041 tgcgctcgac gcattgcacc ccgctcatac cattacggcg atagttaggc tcttcaaccc
     5101 agtcgccctc tgttgcccac cagtgattaa attcgtcgta ctttgctgaa actgccatac
     5161 cctatcgcct gtcattttta ttaacgacaa tgactatagg tggttacctg aggaaaatct
     5221 taatgaaacg tgtcgtatta atgaatttct tttgccgcta ctagtgtgac accgcttttg
     5281 cccttcattt gcagcgcata gattccacca gccacgacca ctaatagtgc gcccagcaga
     5341 taaaatgtga atttaatcag gtggatacag cgatgaattt tattcgacag ggattaggca
     5401 tcgccttatt accagagtta acgctgaaaa ccattgcagg tgaattgtgt tccgttcctc
     5461 acgaaccaac cttctatcga ca
//
LOCUS       DAZOYL010000250         5472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZOYL010000250 DAZOYL010000000
VERSION     DAZOYL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.251"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="ACHUOY_003330"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="ACHUOY_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR0684022.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="ACHUOY_003331"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="ACHUOY_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR0684023.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="ACHUOY_003332"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="ACHUOY_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR0684024.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="ACHUOY_003333"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="ACHUOY_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR0684025.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="ACHUOY_003334"
     CDS             complement(2270..2761)
                     /locus_tag="ACHUOY_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR0684026.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHUOY_003335"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHUOY_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR0684027.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHUOY_003336"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHUOY_003336"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR0684028.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHUOY_003337"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHUOY_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR0684029.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT         1484 a         1357 c         1274 g         1357 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tc
//
LOCUS       DAZOYL010000251         5463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZOYL010000251 DAZOYL010000000
VERSION     DAZOYL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.252"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            206..775
                     /locus_tag="ACHUOY_003338"
     CDS             206..775
                     /locus_tag="ACHUOY_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0684030.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            1503..1676
                     /locus_tag="ACHUOY_003339"
     CDS             1503..1676
                     /locus_tag="ACHUOY_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR0684031.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(1934..3262)
                     /gene="rutG"
                     /locus_tag="ACHUOY_003340"
     CDS             complement(1934..3262)
                     /gene="rutG"
                     /locus_tag="ACHUOY_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR0684032.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3283..3777)
                     /gene="rutF"
                     /locus_tag="ACHUOY_003341"
     CDS             complement(3283..3777)
                     /gene="rutF"
                     /locus_tag="ACHUOY_003341"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR0684033.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(3788..4378)
                     /locus_tag="ACHUOY_003342"
     CDS             complement(3788..4378)
                     /locus_tag="ACHUOY_003342"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR0684034.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4388..5188)
                     /gene="rutD"
                     /locus_tag="ACHUOY_003343"
     CDS             complement(4388..5188)
                     /gene="rutD"
                     /locus_tag="ACHUOY_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR0684035.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5196..>5463)
                     /gene="rutC"
                     /locus_tag="ACHUOY_003344"
     CDS             complement(5196..>5463)
                     /gene="rutC"
                     /locus_tag="ACHUOY_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0684036.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1438 a         1430 c         1372 g         1223 t
ORIGIN      
        1 agccaaatat cattgacttt ctcggtgaat tgctataaag gcatctatct aggtctttaa
       61 ctgtataaat acacatgaca acaaacgccc gttaagtgtc tatataaaaa ggtagctgta
      121 gctatagaca acaattgtga taattgattt aatggctata gacaagcaag acagaccttt
      181 gttgactata tagagagata agaacatgcg tacattcctt tatgctcgtg tatcagttga
      241 tgacctcacc acacagaacc aagtagaagc tgttaaacgt gctggttatg aagtcagacc
      301 ttcacgagta attgaggaag ttattagtgg ggctacacca gctatggaca gaccacaatt
      361 tgtaaagctg gtggataagc tggaagaagg tgattgtctt gtagtgacca agattgaccg
      421 cttaggaagg gataacattg acgtacagaa gaccgtcaca atgttacttg gtatgggtgt
      481 taagctggtc tgtctagatt tgcctgtcca agacctttca agtgctgaag gtaaacttat
      541 tcttcaacta ttcagtacct ttgcagagtt tgaacttaac cgcatcagag agcgaaccag
      601 agagggctta gaacgagcta aagcacaagg taaggtgttg ggtcgtcctg tagctaaaaa
      661 cactacagag gccgttttag agcataaggc taagggattg tcacaaagtc aggttgcaaa
      721 gcttctgaat ctctctgtga gaacggttag ccgtcactgg acaaagacac tgtaaaaaaa
      781 acttagtagc atcttcatag cctccttcat gggggctttt ttgtgtctgt aacatatggt
      841 gaccaaacat ctatatatcg ttatatagcc ctgtatatag cactatatat agtgtctgga
      901 gagggacgac taacaccctg catagttccc tgaaaagggg cttcacagat tctctatata
      961 tttcaataag ttaggataaa aagggactat accgatactg catagaatct gtgtaggatt
     1021 gcatagtcat aggggttgga acaagcccaa ctacacctga taaagcctgt tattaacagg
     1081 tagttgaatg tacgtgttta gctatgtaaa agcaccagaa ctttagccat ttctaaccac
     1141 tcctcgtgtt atctctattc cgtagcgatt cgctaccact tatttaaaga taagacgtcc
     1201 ttttcagagt gcaaatttca caaccactta tttgatttat aacaactttc acaagcacgt
     1261 aattttgtcg caaaatgaca catttttatc tcatcgcgtt tttttaatca taagagcggc
     1321 ttatggataa ttattggaga tgatatctat tctcgctaag aagctgttgc aggatattac
     1381 caaacgcggg tctacccgcg tcagttcact aagcttagtc ccacgtagcg aaaatatggc
     1441 agccgccata cgccgcgtta attctatgca atatgatgtc tatacccaga cggaggtcag
     1501 taatggcaaa ccatcgaggc ggttccggca attttgcaga agaccgcgaa agagcatcag
     1561 aagcaggtaa aaaaggtgga cagcacagcg ggggtaattt caagaatgac ccgcagcgcg
     1621 catctgaagc aggtaaaaaa ggtggcaaga gcagtcacgg caaaagcgac aactagccgg
     1681 gctaatcaat gacgaatgca tttttgtctg tagctcgtca aaaagccatc accgccggtt
     1741 acccggtggt tgatactgat gacaaatgta agcttgcctg atgcgcgatg cttatcaggc
     1801 ctaccagaag attgcaatat attgaatttg cactgttttg taggccggat aaggcgttta
     1861 cgccgcatcc ggcatgaaca atgcgtacgt tgtcaacaat ctgcaccgcc gggtaacccc
     1921 ggcggttttc tgtttatggc tcctgatgaa caacttctgg cggtggaacg tcaaccaacc
     1981 ttctacttaa caacgcattg agtaaaatcg cgccaaaggt ggctgtaccg atccctccca
     2041 acgtaaaacc gcccagcgtg agagcaaaat cacccgcgcc cagcactaag gttactgcga
     2101 ccataatcaa attaccgttc tggcttaaat cgacacggtt ttgtacccag atccttgcgc
     2161 ctgcgacggc aatcagcccg aacacaacaa ttgatgcacc accaatcacc gcggccggaa
     2221 tggtatggat cagcgcgcca aatttcggcg aaaagcccaa cagcatggcg atgacggcag
     2281 cagcaacaaa caccagcgtc gagtagactt ttgtcacggc catcacaccg atattttcag
     2341 cataggtggt gacgccgcta ccgccgacag agccggaaag catcgttgcc agaccatcac
     2401 cgacgaatgc tcgccccata tacgggtcca tattgcgtcc ggtcatcccg gcgaccgcct
     2461 tgagatgacc taagttttcc gccaccagaa tcaccgccac gggcgcaatc agcatcattg
     2521 cctgactatt aaaagcagga gtggaaaaat gtggcaaacc gaaccaggca gcatggctga
     2581 cgagagtaaa atcgacggct tttcccagcc ctaaaacgtt ggtcatcacg ccatacagca
     2641 gacaggcgac aattaaacct acgagaatca ataaccgctg gatcatgcca cgggtaaaca
     2701 ccgccaccag cccaatacac agcaccgtca ttaccgccat ccagctatca aaggctgaag
     2761 ccgatacact tttcactgcg ataggcgcta agttcaggcc aatcgccatc accaccgcac
     2821 ccgtcaccac cggcggcatt agtcgttcaa tccagcgtgt accaattttc atcaccacca
     2881 ggccaatgac ggtataaacc agcccacagg cgataatccc gcccagtgcg atgctgatat
     2941 tcgggttaat gccctgaccg ttaaagcccg tcgcggcaat caccacgccg acaaaagccg
     3001 cgctggagcc gagataactg gggacgcgcc cgccggtaat aaagaaaaac agtaacgtgc
     3061 cgatccccga cattaaaatg gaaagattgg gatccagccc catcagaatc ggcattaaca
     3121 ccgtcgcgcc aaacatcgcc accgcgtgtt gaacgcccat tactgccgtc tgagcaaacg
     3181 gcaatcgttc atccggcgcg accacgccgc tctctgtaga ggtcgatttt aactgccagt
     3241 gaggaaaacc gaacattgcc atcagctgtc tccttaagga ggttaacaag cagggcgcat
     3301 cagcgcgtga taactgcgat cgaaccacac cagcccgtag ggtgtggtgt gacgatgaat
     3361 cgcttcgatg gcgcaaaaca gaatgtcgtg ggtgccgacg ctcaccacct ggctgatgcg
     3421 gcagtcaaac gaaaccagag cctcttccag ttgcgggcat ccggtcacac ccgtctgcca
     3481 gcgggcggcg gcaaagcggt gttccatggg cgttttgccg ccaaaaagat ttgaaagcgg
     3541 ctcctgcccg gcgctcagtg tatttacaca cagcgttcga ttttcattga ataccggcca
     3601 gacggacgcc ccacgattca ggcacaccag taatgtgggc ggcgtgtcgg tcacgctgca
     3661 gacggcgctg gcggtgaacc cggcgcgccc ggatggaccg tccgtggtga tgatattgac
     3721 cgccgcgccc atacaggaca tcgcatcgcg aaaggtttgt tgatcaacta tgttcatagt
     3781 ttgctcctta caacagcccg caggcttctt caaaggacag acgtggcagg cgcgcaaaaa
     3841 gtttgctgct atcgccatag ccgatattaa tcagcagatt gctcttcagc gtgctgcccg
     3901 caaaaaaggc gtcgtcaacg tgttgacggt caaaacccga catcgggccg gtatccagtc
     3961 ccagcgcacg gcaagcgacg atcagatagg ccgcctgcat ggagctgttg cgaaacgctg
     4021 tttcttcggc aagttgtggg ctggaggtaa accaactgcg ggcatcaccg tggggaaaca
     4081 gtagtggtaa ccgttcataa aattcactgt cccaggcaac gatagcggtg acgggcgcgg
     4141 tcagggtttt ttgtagattg ccgctggaaa gcgccgggtg cagacgttct tttccttcta
     4201 cagtgcggat aaacacgatc cgtgccggag aacagttagc tgatgtcggc ccccatttta
     4261 tcggggcata aagctcccgt aacgtctcat cgctgacggg tgtctcccgc cagccgttgt
     4321 gagtgcgggc atcggtgaac agggtgctaa gcgcacctgg gctaacggct tcgttcatag
     4381 caattcctta cagggcggct tcacggtgat gtaacaggct ggcaagcccg ttgagtaaca
     4441 gagcattaaa cgtttcgggg tcggtcacgt tgcaggcgtg tccgccatag cgcatcacca
     4501 ttttctggct atcgggcagg gcggcatgaa gctcactgga acatgctgat ggcaccagca
     4561 gatcatcact ggcgcagatg atttgcaccg ggcagcggat gcgatccgca tggtgactaa
     4621 agtcagcgcg tttgagggcg ttaagtcgac gcagtaaatt atttttgccc tgaaaatgcg
     4681 ccagtgccag cgcgtcttct gcctcaaggc gaggtgcgcg ggccgccatc cagtcggcgg
     4741 gatagaggaa caacggctgc gcttccaccc atgcctgcgc gccgccgcta tacagtaatc
     4801 gttcgcgaac ctgaaaacag cggcgcgtat gggcgtttat tcgtagccag ccgttaacgc
     4861 tgatcagcac agttaccgac gcgggataat ccagcgccag ctgcattccc accagcgcac
     4921 cgagcgcatg gccgaccacc gcgtaatgct caatccctgc ggctaccagc gcctgatgca
     4981 gttccgctgc catctgggcg atactgtaat cttctgccag cgtgtcggga ttattgccgg
     5041 tgccgcgctg gtcgtaacag actacctgat actcctgctc cagcaccgcc agttgcggta
     5101 accagtaact gccgctaccg ccaagacccg aaatcaacac cactacgggc gcatcagcat
     5161 aagggggagg tgagagtgaa agtttcatcg cggcctcact tggcgatatg cgcaattgtg
     5221 gcgatttcca ccagcgcgtc aggttttacc agtccgcact gaatgcagaa tcgcgccggt
     5281 ttatcacccg gaaaaaactc ggcgtagatt tcgttaatcg cggcgtaatt tttccagtcg
     5341 gtaataaaga tgctgttgaa ggtcacatcc gccatcgtgc cacccgccgt ctcgatcacc
     5401 ttgcggatag tttccagaac gtggcgggtt tgcgcctttg ggtcatcggc aaacagcacg
     5461 tta
//
LOCUS       DAZOYL010000252         5455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZOYL010000252 DAZOYL010000000
VERSION     DAZOYL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.253"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUOY_003345"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUOY_003345"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR0684037.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUOY_003346"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUOY_003346"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR0684038.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHUOY_003347"
     CDS             complement(2698..3390)
                     /locus_tag="ACHUOY_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR0684039.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUOY_003348"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUOY_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR0684040.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1404 a         1394 c         1279 g         1378 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgctttac atatatttgc attaataaaa taattgtaat tataaggtta
     5401 aatatcggta atttgtattt aataaatacg atcgatattg ttactttatt cgcct
//
LOCUS       DAZOYL010000253         5331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZOYL010000253 DAZOYL010000000
VERSION     DAZOYL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.254"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            319..3024
                     /gene="malT"
                     /locus_tag="ACHUOY_003349"
     CDS             319..3024
                     /gene="malT"
                     /locus_tag="ACHUOY_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR0684041.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3067..4083)
                     /gene="rtcA"
                     /locus_tag="ACHUOY_003350"
     CDS             complement(3067..4083)
                     /gene="rtcA"
                     /locus_tag="ACHUOY_003350"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR0684042.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4087..5313)
                     /gene="rtcB"
                     /locus_tag="ACHUOY_003351"
     CDS             complement(4087..5313)
                     /gene="rtcB"
                     /locus_tag="ACHUOY_003351"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR0684043.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT         1266 a         1501 c         1328 g         1236 t
ORIGIN      
        1 aatatccaat gtgctccatc tcattcttaa tagatttatt aagatcatct ttttagatgg
       61 cactttcatc aggaatgaag aagaaaccct tgcttaaatg aatctgatga acataaggga
      121 aaccagtatt cacgctggat cagcgtcgtt ttaggtgagt tgttaataaa gatttggaat
      181 tgtgacacag tgcaaattca gacacataaa aaaacgtcat cgcttgcatt agaaaggttt
      241 ctggccgacc ttataaccat taattacgaa gcgcaaaaaa ataatatttc ctcattttcc
      301 acagtgaagt gattaactat gctgattccg tcaaaactaa gtcgtccggt tcgactcgac
      361 cataccgtgg ttcgtgagcg cctgctggct aaactttccg gcgcgaacaa cttccggctg
      421 gcgctgatca cgagtcctgc gggctacgga aagaccaccc tcatttccca gtgggcggca
      481 ggcaaaaacg atatcggctg gtactcgctg gatgaaggtg ataaccagca agagcgtttc
      541 gccagctatc tcattgccgc cgtgcagcag gcaaccaacg gtcactgtgc gatatgtgag
      601 acgatggcgc aaaaacggca atatgccagc ctgacgtcac tcttcgccca gcttttcatt
      661 gagctggcgg aatggcatag cccactttat ctggtcatcg atgactatca tctgatcact
      721 aatccagtga tccacgagtc aatgcgcttc tttattcgcc atcaaccaga aaatctcacc
      781 ctggtggtgt tgtcacgcaa ccttccgcaa ctgggcattg ccaatctgcg tgttcgtgac
      841 caactgctgg aaattggcag tcagcaactg gcatttaccc atcaggaagc gaagcagttt
      901 tttgattgcc gtctgtcatc gccgattgaa gccgcagaaa gcagttggat ttgcgatgac
      961 gtttccggtt gggcgacggc actacagcta atcgccctct ccgcccggca gaatacccac
     1021 tcagcccata agtcggcacg ccgcctggcg ggaatcaatg ccagccatct ttcggattat
     1081 ctggtcgatg aggttttgga taacgtcgat ctcgcaacgc gccattttct gttgaaaagc
     1141 gccattttgc gctcaatgaa cgatgccctc atcacccgtg tgactggcga agaaaacggg
     1201 caaatgcgtc tcgaagagat tgagcgtcag gggctgtttt tacagcggat ggatgatacc
     1261 ggcgagtggt tctgctatca cccgctgttt ggcaacttcc tgcgccagcg ctgccagtgg
     1321 gaactggcgg cggagctgcc ggaaatccac cgtgccgccg cagaaagctg gatggcccag
     1381 ggatttccca gcgaagcgat tcatcatgcg ctggcggcag gcgatgcgct gatgctgcgc
     1441 gatattctgc ttaatcacgc ctggagtttg ttcaaccata gcgaactgtc gctgctggaa
     1501 gagtcgctta aggccctgcc gtgggacagt ttgctggaaa atccgcagtt agtgttattg
     1561 caggcgtggc taatgcaaag ccaacatcgc tatggcgaag ttaacaccct gctagcccgt
     1621 gctgaacatg aaatcaagga catcagagaa ggcaccatgc acgcagaatt taacgctctg
     1681 cgcgcccagg tggcgattaa cgatggtaat ccggatgaag cggaacggct ggcaaaactg
     1741 gcactggaag agctgccacc gggctggttc tatagccgca ttgtagcaac ctcggtgctg
     1801 ggtgaagtgc tgcactgcaa aggcgaattg acccgctcac tggcgctaat gcagcaaacc
     1861 gaacagatgg cacgccagca cgacgtctgg cactacgcct tatggagttt aatccagcaa
     1921 agtgaaattc tgtttgccca agggttcctg caaaccgcgt gggaaacaca ggaaaaaacg
     1981 ttccagctga tcaacgagca gcatctggaa cagctgccga tgcatgagtt tctggtgcgc
     2041 atccgagcac aactgttatg ggcctgggca cggctggatg aagccgaagc gtcggcgcgt
     2101 agcgggattg aagtcttgtc gtcttatcag ccacagcaac agcttcagtg cctggcaatg
     2161 ttgattcaat gctcgctggc ccgtggtgat ttagataacg cccgtagcca gctgaaccgt
     2221 ctggaaaacc tgctggggaa tggcaaatat cacagcgact ggatctctaa cgccaacaaa
     2281 gtccgggtga tttactggca aatgaccggc gataaagccg ccgctgccaa ctggttgcgt
     2341 catacggcta aaccggagtt tgcgaacaac cacttcctgc aaggtcaatg gcgcaacatt
     2401 gcccgcgcac aaatcttgct gggcgagttt gaacctgcgg aaattgttct cgaagaactc
     2461 aatgaaaatg cccgcagtct gcggttgatg agcgatctca accgtaacct gttgctgctt
     2521 aatcaactgt actggcaggc cggacgtaaa agtgacgccc agcgcgtgtt gctggacgca
     2581 ttaaaactgg cgaatcgcac cggatttatc agtcattttg tcatcgaagg cgaagcgatg
     2641 gcgcaacaac tgcgccagct gattcaactc aatacgctgc cggaactgga acagcatcgc
     2701 gcgcagcgta ttctgcgaga aatcaatcaa catcatcggc ataaattcgc ccatttcgat
     2761 gagaatttcg ttgaacgtct gctgaatcat ccggaagtgc cagaactgat ccacaccagc
     2821 ccgttgaccc aacgtgaatg gcaggtcctg gggctgatct actccggtta cagcaatgag
     2881 caaattgcgg gcgagctgga agtggcggca accaccatca agacgcatat ccgcaatctg
     2941 taccaaaaac tcggcgtcgc ccaccgtcag gccgcggtac aacacgcgca gaaactgctg
     3001 aagatgatgg gatatggggt gtgagtttag ccggataatg cattggatcc ggtttacatc
     3061 tttacatcat tcaatactca cccgcgttac gccatctgtt tctatcaaac caaaccgcac
     3121 cggcaagaaa cgctccacca cggcgatatt ggtcagcaga tggcatgaag gatgggcgac
     3181 cgtaaattct cccgcgcccg ccagcgccat cggtagcacc agctggtcgg cgagatattc
     3241 ccccactacc gccgggcttg ccaggtagcg tttcacctct ttcactaact gcgcggctac
     3301 cacctcggca ctgacgcgct tttcaccgac gacgaaaaag cgttcagtga tattttcact
     3361 ttcgacttcc agcgagacgg tattacccgg cccctggtcg cgcggcaggt tatgaatatt
     3421 ctgttcatgc agggaaaaac tccccgccag tgtagcgatt tcacgctcag caacatggcg
     3481 cggcacacca gctaatagaa cttctccacg catctgcaca atgttcccgc gctcgccaag
     3541 ttgcaaggtg ttaaacgatg ccacgggcga gacttccgtt gccaccacgc cgcctccggc
     3601 aggataaaaa ccgtgacgta acagcgtggt ttgctgatga attcctattt tcgccagcag
     3661 cggctccagc acccggcgga taaaatccgc aggcggggcc gacgggttat cggtgcctcc
     3721 gctcacttca acacgcgaag gtccatcggc aaaccacagc gcgggcagca ccgtttgcag
     3781 caccagcgta cagcttccgg cactaccgat agcaaagcgg tactcgccgc cgcgcacggt
     3841 gccaagccgg aagaccagac gctgcgaccc cagctccgcg ccttccaccg ttgccctaca
     3901 aatttccgca gccgctttta ccgcggtcag atgctggcgc aacagccccg gtttcgcccg
     3961 cccggcacga atgctggtga tggtaaatgg ctggccggtt atcatcgaca ggctcagcgc
     4021 cgagcgcagg atctgcccgc caccttcgcc ctgtgcgcca tccagcgcaa tcatcctttt
     4081 catcatttat ccttttacgc acaccacctg acgcagggta tagataactt ccaccagatc
     4141 gctttgtgcc gccatcaccg catcaatatc tttatacgcc atcgggattt cgtcgatcac
     4201 ttcggcatct ttacggcatt ccacatgcgc ggtggcacga atttgatctt ccacgctgaa
     4261 cagttttttc gctttagtac ggctcattac ccgcccggca ccgtggctgc acgaacagaa
     4321 agattcttca tttcccagcc cacggacgat aaagcttttt gctcccatcg accctggaat
     4381 aattccatat tgaccagcac gcgcaaacac cgcgccttta cgcgtcacgt agatctcttc
     4441 accaaagtgc tgttcttttt gcacatagtt gtggtgacag ttgatctctt ccatcgccag
     4501 cgtttgtggc tgtctgaccg ttttctgcgt aatgctctgc aatgccgtta ccacgttttc
     4561 catcatcgca tcgcggttaa ggctggcgaa aagctgcgcc caggccacgg ctcttaggta
     4621 atcatcaaag tattccgtac cttccataaa gtacgccaga tcacgcgacg gcaacgtctc
     4681 aagttggtcc tgcatctctt tttgcgccag atcgataaag taagtcccga tggcgttacc
     4741 aattccgcgt gaaccggagt gcagcataat ccacacctgc tccgactcat caaggcagat
     4801 ttcaataaag tggttacccg ttcccagcgt tcccaggtgt ttatagttgt tagtgttaag
     4861 gaaacgcgga tatttttgcg ttaaccactg ataaccggcc tccagctcag cccatttcgc
     4921 atcgacgtta aaaggcgggt tttcccatgc gcctttatca cgtttacaac gcccggtagt
     4981 acgcccgtgt ggtacggccg tttcaatcgc ttgacgcagc tccgccaggt tttcaggcag
     5041 gtctgccgcc gttaacgcgg tacgcagcgc gttcattcca cagccaatat ccacgcccac
     5101 cgccgccgga ataatcgccc ctttggtcgg gatcacgcta ccaatggtgg aacctttacc
     5161 caggtgtaca tcaggcatta ccgcaatatg tttgaaaata aacggcatct tcgccgtatt
     5221 aataagttgc tgacgcgcat cggcttctac cggcacgcct ttggtccaca tttttaccgg
     5281 ggcattttca gtggtcagta attcgtaatt cattttgttt ttctcttttc g
//
LOCUS       DAZOYL010000254         5317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZOYL010000254 DAZOYL010000000
VERSION     DAZOYL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.255"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            120..1478
                     /gene="mltD"
                     /locus_tag="ACHUOY_003352"
     CDS             120..1478
                     /gene="mltD"
                     /locus_tag="ACHUOY_003352"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR0684044.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1527..2150)
                     /locus_tag="ACHUOY_003353"
     CDS             complement(1527..2150)
                     /locus_tag="ACHUOY_003353"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0684045.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(2154..2954)
                     /locus_tag="ACHUOY_003354"
     CDS             complement(2154..2954)
                     /locus_tag="ACHUOY_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0684046.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3195..4109
                     /gene="yafC"
                     /locus_tag="ACHUOY_003355"
     CDS             3195..4109
                     /gene="yafC"
                     /locus_tag="ACHUOY_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR0684047.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4106..4909)
                     /gene="dkgB"
                     /locus_tag="ACHUOY_003356"
     CDS             complement(4106..4909)
                     /gene="dkgB"
                     /locus_tag="ACHUOY_003356"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR0684048.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5072..5148)
                     /locus_tag="ACHUOY_003357"
     tRNA            complement(5072..5148)
                     /locus_tag="ACHUOY_003357"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5112..5114),aa:Asp,seq:gtc)
BASE COUNT         1394 a         1286 c         1318 g         1319 t
ORIGIN      
        1 cctgaagagc gttttgcatg gttaaggtca aagaaagata ggttctgata aaactttctt
       61 gtcatcggct ccgttcgccg ttatgatcgg tcgtctttta agcaactatt gacacacaca
      121 tgaaggcaaa agcgatatta ctcgcctctg tcctgctcgt gggttgccag agtaccggca
      181 acgttcaaca gcacgcacag agcctttctg cagctggtca aggggaagca gcaaagttta
      241 caagtcaggc acgatggatg gacgatggga cgtctatcgc gccagatggt gacttgtggg
      301 ctttcattgg cgacgagcta aagatgggaa ttccggaaaa tgaccggatt cgcgaacaga
      361 aacagaaata tttacgcaat aagagctatc tccacgatgt aactttacgg gcagagccgt
      421 atatgtactg gatagcaggg caagttaaaa aacgtaacat gcctatggaa ctggtactac
      481 tacccatagt ggagagcgct tttgatcctc acgcaacgtc tggcgccaat gccgcaggca
      541 tctggcagat cattccgagc acggggcgca attatggttt gaaacagacc cgcaattatg
      601 acgcgcgtcg cgatgttgtt gcttcaacaa ctgccgcgct gaatatgatg cagcgtctga
      661 acaaaatgtt tgacggcgac tggcttctga ccgtagcggc ttataacagc ggcgaaggtc
      721 gggtcatgaa ggcaattaaa acgaacaaag cgcgtgggaa atccacggac ttctggtcgt
      781 taccgttgcc gcaggaaacg aagcagtacg tgcctaaaat gctggcattg agtgatattc
      841 tcaaaaacag caagcgttat ggcgtacgtc tgccaacgac cgatgaaagc cgtgctctgg
      901 cgcgtgtgca cctgagtagc ccggttgaaa tggcgaaggt tgcagatatg gcggggattt
      961 ccgtcagcaa gctgaagaca ttcaacgctg gcgtgaaagg ctccacgctg ggcgcaagtg
     1021 ggccgcagta cgtgatggtg ccaaagaagc atgcagatca actgcgtgaa tctctcgctt
     1081 caggcgaaat tgctgctgta cagtcgacgc tggttgccga caatacgccg cttaacagcc
     1141 gtgtttacac cgtacgctct ggcgacacgc tttcaagcat cgcttcacgt ctcggcgtaa
     1201 gcaccaaaga tttgcagcag tggaacaaac tgcgcggatc taagctgaag ccaggccaaa
     1261 gtttgacgat tggtgcaggc agtagcgcac agcggttggc aaacaacagc gatagcatta
     1321 cgtatcgtgt gcgcaaaggc gattcgcttt caagcattgc taaacgccac ggcgtgaaca
     1381 tcaaagatgt gatgcgctgg aacagcgaca ctgcgaatct gcaaccaggc gataagctga
     1441 cgttgtttgt gaaaaacaac aacatgccag attcctgaca aaccagataa taaaaaaggc
     1501 accgattccc ccggtgcctt ttttatttat gccgctttat gtgcttctac catgatgata
     1561 tcactggtga aagagccatc agtctgcaag gaaaaatacg ttctcacctc tgtcgatgcg
     1621 ctctgttggt aaatgcgaat agcgtctact aacgcttctg gcgtacgcat tctcacgacc
     1681 catgaagaaa attccagcgg taacttatct gtaattaaat tatcaactat cagattggct
     1741 tcattgatta acgtcaacca cgcaccgctg gcgtagtttc gtacgtgaga ggtatcgcgt
     1801 aatgcttcta ccgcctgtaa ccagatgtcg cgcactgggt gacccggaga cattacatcc
     1861 atcacaatca gcctaccgcc aggtttcaat atcctattca cttctcgcag tgctgcacca
     1921 acatcatgcc agtgatgggc agaataacgg ctgataacaa tatcaaatgc gttatcggca
     1981 aatggcagac ttgcggcata tccctggcgg gtggtgatat ttttcagttg ccgggcttcg
     2041 gcagcttgtg ccacgacatc cagcatgtgg gcagataagt catacgccac caccgcgctc
     2101 acgttttgcg cagcgccaaa cgttggtctg ccctgcggca gacctgacat accttattta
     2161 tcaggcttgc cgggactgaa ttcaacgagt agcggattgt gatcggaagc gcgcgtaacc
     2221 agtacagaag cttcactgac gttcagacca cggtagaaaa caaaatcgag cgggcgacca
     2281 aacgcccgac ggcgctgatc atcggtaaaa cgcacctggc gcagcgacat ttcccgcgca
     2341 aagcgatata acgcgttcat ccttctacgg ctccaggcat tgaaatctcc cgccataatg
     2401 accgggccgc tgtggtgagc tatctgatca ccaataggaa gtaactgctt actatagaca
     2461 tccacgccca gactgaagtt gacggcgtgt atattaacca ccatcaacag gcgggtgtca
     2521 ggtaatggat agaccgtcac cagtgccgac ttcgccagac gcaaaatggg ttctcgttca
     2581 cgtaacgggc agcaatacac tggatgtgcc gccgaaaggg tcattacgcc agaaggatgt
     2641 tgtggcagca cgaaagcggg tacctgatcg gcggcaagat agttagcggt cgcaaactgt
     2701 accaactctg gcgttgtttg cgcttcctgc aataacacca gatgtgcgtc tttgccgtaa
     2761 ttctttaata ccgacaacca ttcagcgcgt tgctgtttgt atatgttcca caccaggatc
     2821 cgaatacgct cttcggtact taacggttct ccgggtggta atgcctggcc gatgctcgca
     2881 aaagaccccg gcggtaagat cctctccgca ggttgtccgg caacatagcg catggcatag
     2941 gtgttttttc gcacttttga ctttgaaacc tctattacgt caacccttcc gcactcggga
     3001 gggttctact gttataggga ttttagctca ctctttcaac gagcaattgt atattgttat
     3061 gtaagcaagt gcttacaccg gtcagaaatt tgctcattat tgagaacatt aaatcggatg
     3121 acatatcacg catcctgatg gtttaatgac aaataaattc aaaatatctc atctattttg
     3181 caacaggagc aaaaatgaaa gccacgtcgg aagaactcgc catttttgtt tcggtcgtcg
     3241 aaagcggcag ctttagccgg gcagcggaac aattagggca agcaaactca gcggtaagcc
     3301 gggcggtgaa aaagctggaa atgaaacttg gcgttagcct gcttaatcgg accacgcggc
     3361 aacttagcct gacggaagaa ggcgagcgtt attttcgtcg cgtacagtca attttgcagg
     3421 agatggcagc ggcagaatca gaaattatgg agacgcgtaa tacaccgcgt ggactgttac
     3481 ggatcgatgc cgcaactcca gtggtgctgc actttctgat gccgttaatt aagcctttcc
     3541 gtgaacgcta tccggaagtc actttatcgc tagtctcctc cgaaacgatt attaatttga
     3601 tcgaaagaaa agtggatgtc gcgatacgcg ctggtacgtt aacggactcc agcttacgtg
     3661 ccaggccgtt atttaacagt tatcgaaaaa ttatcgcctc ccccgattat atttcccgct
     3721 acgggaagcc agaaacgatc gacgatttaa agcaacatgt ttgcctggga ttcactgaac
     3781 ccgcttccct caatacctgg ccgatagcct gtagtgatgg acaattacat gaggtgaagt
     3841 acggtttgtc atccaatagt ggggaaacac tgaaacagct ttgcctgagt ggcaacggga
     3901 ttgcgtgttt gtccgactac atgatcgaca aagaaatcgc tcgcggagaa ttggtggagt
     3961 taatggcaga taaagtgttg ccagtggaaa tgccattcag tgccgtctat tacagcgacc
     4021 gtgcggtaag tacgcgcatc cgggctttta tcgatttcct tagcgagcat gtaaaaacag
     4081 ctcccggagg agctgtcaga gaggcttaat cccattcagg agccagacct tccgggctaa
     4141 ccaggcggtc gttgcaatcc agtgcggcga tcgctttttt atcttcggca tcaagctgta
     4201 aattttgtgc cttaagatta ctttccaggt ttttacgttt agtagaagaa ggaattactg
     4261 agtaaccttc ccccatagcc cacgccagaa tcacttgtgc cggagtcgca ttgtgtttag
     4321 ctgcaatacg agcaataacc tcatctttca gtgccttacc ataagccagc gtcatatagg
     4381 aagtgatatg aataccgtgc tgtttagccc aggcaaccac tttacggttt tgcagataag
     4441 gagagagttc aatctggttg gtagcgatgt tttcagcgcc aacagcagca atcgcttttt
     4501 ccatcaacgg gatcgtgaag ttggaaatac cgatctcacg tgtcagacct tgttttttgg
     4561 cctccagcag cgcctgcata aactcttcaa cagagacttc atcgtttggt gacggccagt
     4621 ggattagcgt cagatcaaca taatcggtac gcaatttttg caggctctct tttagactcg
     4681 ggatcaattt gtctttgctg agattttcaa tccagatttt agtggtgatg tagagttcat
     4741 gacgtggcac gccactttct gcaatcgcct gacctactgc ggcttcgtta tcatagattt
     4801 gtgcagtatc aattgcgcga taaccaagtt caagcgccgt tttcacagat gaaataacaa
     4861 cgtcgtcttt gagacggaaa gtacctaaac caaatgcagg gatagccata atatgcctct
     4921 tttaatgcta ttttcgttaa ctgaggtgat tatgggcttc agactgttgc ggaaaaagag
     4981 cataaaatgc agaggatttt tgcgattctg gcaataatag atatacagac gaaaaaaaac
     5041 ctcgagcgtt aactcgaggc ttcttaataa gtggcggaac ggacgggact cgaacccgcg
     5101 accccctgcg tgacaggcag gtattctaac cgactgaact accgctccac cgaattcttt
     5161 tacaaccacc ggttttatga ccggcttact gcttaatttg atgcctggca gtttatgaat
     5221 cacttcgggc tgttgcttcg caacgttcaa aagctttcgc ttttgtccta ctctcttatc
     5281 gagtagtgaa ctgcctcaaa ggtcagtgcc attcttc
//
LOCUS       DAZOYL010000255         5296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZOYL010000255 DAZOYL010000000
VERSION     DAZOYL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.256"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..130)
                     /locus_tag="ACHUOY_003358"
     CDS             complement(<1..130)
                     /locus_tag="ACHUOY_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0684049.1"
                     /translation="MDTRVAFMTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPS"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="ACHUOY_003359"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="ACHUOY_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHUOY_003360"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHUOY_003360"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR0684050.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHUOY_003361"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHUOY_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR0684051.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="ACHUOY_003362"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="ACHUOY_003362"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR0684052.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5296)
                     /locus_tag="ACHUOY_003363"
                     /pseudo
     CDS             complement(4172..5296)
                     /locus_tag="ACHUOY_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1384 a         1331 c         1261 g         1320 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagc
//
LOCUS       DAZOYL010000256         5295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZOYL010000256 DAZOYL010000000
VERSION     DAZOYL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.257"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            138..995
                     /gene="focA"
                     /locus_tag="ACHUOY_003364"
     CDS             138..995
                     /gene="focA"
                     /locus_tag="ACHUOY_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR0684053.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1050..3332
                     /gene="pflB"
                     /locus_tag="ACHUOY_003365"
     CDS             1050..3332
                     /gene="pflB"
                     /locus_tag="ACHUOY_003365"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0684054.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3524..4264
                     /gene="pflA"
                     /locus_tag="ACHUOY_003366"
     CDS             3524..4264
                     /gene="pflA"
                     /locus_tag="ACHUOY_003366"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR0684055.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4396..4533)
                     /locus_tag="ACHUOY_003367"
                     /pseudo
     CDS             complement(4396..4533)
                     /locus_tag="ACHUOY_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            4633..>5295
                     /locus_tag="ACHUOY_003368"
     CDS             4633..>5295
                     /locus_tag="ACHUOY_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0684056.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     ILD"
BASE COUNT         1360 a         1307 c         1275 g         1353 t
ORIGIN      
        1 aaatgtttac tccgtatctg cataaaaacc atgcgagtta cgggcctata agccaggcga
       61 gatatgatct atatcaattt ctcatctata atgctttgtt agtatctcgt cgccgactta
      121 ataaagagag agttagtgtg aaagctgaca acccttttga tcttttactt cctgctgcaa
      181 tggccaaagt ggccgaagag gcgggtgtct ataaagcaac gaaacatccg cttaagactt
      241 tctatctggc gattaccgcc ggtgttttca tctcaatcgc attcgtcttc tatatcacag
      301 caaccactgg cacaggcaca atgcccttcg gcatggcaaa actggttggc ggcatttgct
      361 tctctctggg gctgattctt tgtgttgtct gcggagccga tctctttact tccaccgtgt
      421 tgattgttgt tgctaaggcg agtgggcgca tcacctgggg tcagttggcg aaaaactggc
      481 taaatgtcta ttttggcaac ctggtcggcg cactgctgtt tgtactttta atgtggcttt
      541 ccggcgagta tatgaccgca aatggtcaat ggggactaaa cgtcctacaa accgccgacc
      601 acaaagtgca ccatactttt attgaggccg tctgtcttgg tatcctggca aacctgatgg
      661 tatgtctggc agtatggatg agttattctg gccgcagcct gatggacaaa gcgttcatta
      721 tggtgctgcc ggtcgcgatg tttgttgcca gcggttttga gcacagtatc gcaaacatgt
      781 ttatgatccc gatgggtatt gtaatccgcg acttcgcatc cccggaattt tggaccgcag
      841 tcggttctgc accggaaaat ttttctcacc tgaccgtgat gaatttcatc actgataacc
      901 tgattccggt tacgatcggc aacattatcg gtggtggttt gttggttggg ttgacatact
      961 gggtcattta cctgcgtgaa aacgaccacc attaatggtt gtcgaagtac gcagtaaata
     1021 aaaaatccac ttaagaaggt aggtgttaca tgtccgagct taatgaaaag ttagccacag
     1081 cctgggaagg ttttaccaaa ggtgactggc agaatgaagt aaacgtccgt gacttcattc
     1141 agaaaaacta cactccgtac gagggtgacg agtccttcct ggctggcgct actgaagcga
     1201 ccaccaccct gtgggacaaa gtaatggaag gcgttaaact ggaaaaccgc actcacgcgc
     1261 cagttgactt tgacaccgct gttgcttcca ccatcacctc tcacgacgct ggctacatca
     1321 acaagcagct tgagaaaatc gttggtctgc agactgaagc tccgctgaaa cgtgctctta
     1381 tcccgttcgg tggtatcaaa atgatcgaag gttcctgcaa agcgtacaac cgcgaactgg
     1441 atccgatgat caaaaaaatc ttcactgaat accgtaaaac tcacaaccag ggcgtgttcg
     1501 acgtttacac tccggacatc ctgcgttgcc gtaaatctgg tgttctgacc ggtctgccag
     1561 atgcatatgg ccgtggccgt atcatcggtg actaccgtcg cgttgcgctg tacggtatcg
     1621 actacctgat gaaagacaaa ctggcacagt tcacttctct gcaggctgat ctggaaaacg
     1681 gcgtaaactt ggaacagact atccgtctgc gcgaagaaat cgctgaacag caccgcgctc
     1741 tgggtcagat gaaagaaatg gctgcgaaat acggctacga catctctggt ccggctacca
     1801 acgctcagga agctatccag tggacttact tcggctacct ggctgctgtt aagtctcaga
     1861 acggtgctgc aatgtccttc ggtcgtacct ccaccttcct ggatgtgtac atcgaacgtg
     1921 acctgaaagc tggcaagatc accgaacaag aagcgcagga aatggttgac cacctggtca
     1981 tgaaactgcg tatggttcgc ttcctgcgta ctccggaata cgatgaactg ttctctggcg
     2041 acccgatctg ggcaaccgaa tctatcggtg gtatgggcct cgacggtcgt accctggtta
     2101 ccaaaaacag cttccgtttc ctgaacaccc tgtacaccat gggtccgtct ccggaaccga
     2161 acatgaccat tctgtggtct gaaaaactgc cgctgaactt caagaaattc gccgctaaag
     2221 tgtccatcga cacctcttct ctgcagtatg agaacgatga cctgatgcgt ccggacttca
     2281 acaacgatga ctacgctatt gcttgctgcg taagcccgat gatcgttggt aaacaaatgc
     2341 agttcttcgg tgcgcgtgca aacctggcga aaaccatgct gtacgcaatc aacggcggcg
     2401 ttgacgaaaa actgaaaatg caggttggtc cgaagtctga accgatcaaa ggcgatgtcc
     2461 tgaactatga tgaagtgatg gagcgcatgg atcacttcat ggactggctg gctaaacagt
     2521 acatcactgc actaaacatc atccactaca tgcacgacaa gtacagctac gaagcctctc
     2581 tgatggcgct gcacgaccgt gacgttatcc gcaccatggc gtgtggtatc gctggtctgt
     2641 ccgttgctgc tgactccctg tctgcaatca aatatgcgaa agttaaaccg attcgtgacg
     2701 aagacggtct ggctatcgac ttcgaaatcg aaggcgaata cccgcagttt ggtaacaacg
     2761 atccgcgtgt agatgacctg gctgttgacc tggtagaacg tttcatgaag aaaattcaga
     2821 aactgcacac ctaccgtgac gctatcccga ctcagtctgt tctgaccatc acttctaacg
     2881 ttgtgtatgg taagaaaacg ggtaacaccc cagacggtcg tcgtgctggc gcgccgttcg
     2941 gaccgggtgc taacccgatg cacggtcgtg accagaaagg tgcagtagcc tctctgactt
     3001 ccgttgctaa actgccgttt gcttacgcta aagatggtat ctcctacacc ttctctatcg
     3061 ttccgaacgc actgggtaaa gacgacgaag ttcgtaagac caacctggct ggtctgatgg
     3121 atggttactt ccaccacgaa gcatccatcg aaggtggtca gcacctgaac gttaacgtga
     3181 tgaaccgtga aatgctgctc gacgcgatgg aaaacccgga aaaatatccg cagctgacca
     3241 tccgtgtatc tggctacgca gtacgtttca actcgctgac taaagaacag cagcaggacg
     3301 ttattactcg taccttcact caatctatgt aattagattt gactgaaatc gtacagtaaa
     3361 aagcgtacaa taaaggctcc acgaaagtgg ggcctttttt agcacgagag ccttttttgt
     3421 cagctatcta tactctaagg tgactgccaa aacagactcg acgtagcctt cgagctgcgc
     3481 accaacacgg cctcagatgg gccacatctg gagaaacacc gcaatgtcag ttattggtcg
     3541 cattcactcc tttgaatcct gtggaaccgt agacggcccg ggtattcgct ttatcacctt
     3601 tttccagggc tgcctgatgc gctgcctgta ttgtcataac cgcgacacct gggatacgca
     3661 tggcggtaaa gaagttaccg ttgaagattt aatgaaagag gtggtgacct atcgccactt
     3721 tatgaacgct tccggcggcg gcgttaccgc atccggcggt gaggcaatcc tacaagctga
     3781 gtttgttcgt gactggttcc gcgcctgcaa aaaagaaggc attcatacct gtctggacac
     3841 caacggtttt gttcgtcgtt acgatccggt gattgatgaa ctgctggaag taaccgacct
     3901 ggtaatgctc gatctcaaac agatgaacga cgagatccac caaaatctgg ttggagtttc
     3961 caatcaccgc acgctggagt tcgccaaata tctggcgaac aaaaatgtga aggtgtggat
     4021 ccgctacgtt gttgtcccag gctggtctga cgatgacgat tcagcgcatc gcctcggtga
     4081 atttacccgt gatatgggca acgttgagaa aatcgagctt ctcccctacc acgagctggg
     4141 caaacacaaa tgggtggcaa tgggtgaaga gtacaaactc gacggtgtta aaccaccgaa
     4201 gaaagagacc atggaacgcg tgaaaggcat tcttgagcag tacggtcata aggtaatgtt
     4261 ctaatttccc cgtaaagcgg caactttatt gagttgccgc ttttttattc cgcacttcat
     4321 tctgctacag cgtttttaac acatcttata gtgcatctac catatcccgt acctgagata
     4381 ataacgactg ataatattac gccgatataa atcgagttcc gtcacctgta ttttccgctc
     4441 cattgcccgc tgatggatcg catcagcaat atgtgcggtc aatgaatcat gacgaggatg
     4501 ggcccatacc aaataaatac gttcagactg cataatttta cctccacgtt aagttctgga
     4561 gcgcacttta cgcaggcggc ataataatgt aaacgtgatg attcaggatg agttaatgaa
     4621 ggattttcac taatgcggat gaatatgtct gactttgcca ctttctttgc tgtggcccgt
     4681 aatcaaagct ttcgtgcagc gggcgatgag ttaggcttat cctcgtccgc cattagccat
     4741 agtattaaaa cactggaaca acgtcttaaa attcgcttat tcaatcggac aacccgaagc
     4801 gtatcgttaa cggaagcggg ttctaatctc tatgaacgat tgcgcccagc tttcgacgaa
     4861 attcagatca tgttagatga gatgaatgat tttcgtctga caccaacagg aacgctcaaa
     4921 atcaatgccg cccgggtagc tgcacgtata tttttaatgc ctttactggt tggtttcaca
     4981 cgtgaatatc ctgatattaa agtcgagctc acaactgacg actctcttgt cgatatcgtc
     5041 caacagggct ttgacgctgg cgttcgactt agtggcatcg tcgaaaaaga tatgatcagc
     5101 gtggcgatcg gcccaccagt gaaattatgt gtagcagcaa cgccagagta ctttgcaaga
     5161 tatggcaaac cccgccatcc tcatgatctg cttaatcatc aatgtgttgt atttcgttat
     5221 cccagcggca aacctttcca ctggcaattt gcaaaggagc aggaaattgc tgtcgcaggg
     5281 aatatcattc tggat
//
LOCUS       DAZOYL010000257         5291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZOYL010000257 DAZOYL010000000
VERSION     DAZOYL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.258"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHUOY_003369"
     CDS             complement(<1..170)
                     /locus_tag="ACHUOY_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR0684057.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="ACHUOY_003370"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="ACHUOY_003370"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR0684058.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="ACHUOY_003371"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="ACHUOY_003371"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR0684059.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="ACHUOY_003372"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="ACHUOY_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR0684060.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHUOY_003373"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHUOY_003373"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR0684061.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHUOY_003374"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHUOY_003374"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR0684062.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="ACHUOY_003375"
     CDS             4288..5151
                     /locus_tag="ACHUOY_003375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR0684063.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
BASE COUNT         1228 a         1448 c         1355 g         1260 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgatgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt g
//
LOCUS       DAZOYL010000258         5280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZOYL010000258 DAZOYL010000000
VERSION     DAZOYL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.259"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(38..400)
                     /locus_tag="ACHUOY_003376"
     CDS             complement(38..400)
                     /locus_tag="ACHUOY_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0684064.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUOY_003377"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUOY_003377"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR0684065.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUOY_003378"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUOY_003378"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0684066.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUOY_003379"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUOY_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR0684067.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUOY_003380"
     CDS             complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUOY_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR0684068.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         1308 a         1310 c         1379 g         1283 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac atctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
//
LOCUS       DAZOYL010000259         5280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZOYL010000259 DAZOYL010000000
VERSION     DAZOYL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.260"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHUOY_003381"
     CDS             <1..69
                     /locus_tag="ACHUOY_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684069.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
     gene            complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHUOY_003382"
     CDS             complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHUOY_003382"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR0684070.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHUOY_003383"
     CDS             complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHUOY_003383"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR0684071.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHUOY_003384"
     CDS             complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHUOY_003384"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR0684072.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHUOY_003385"
     CDS             complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHUOY_003385"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR0684073.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHUOY_003386"
     CDS             complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHUOY_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR0684074.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         1142 a         1396 c         1399 g         1343 t
ORIGIN      
        1 gtctactggg taattgcggt tatttctgtt gtcgcattat ttttaaccgc aaaggttaaa
       61 gacgtttgat gacgtggacg atagcggaaa gcccggtcat ttgaccgggc aaggggatta
      121 attcataaac gcaggttgtt ttgcttcata agcggcaatg gcgtcgtcgt gctgcaaggt
      181 aagcccaata ctgtccagac cgttcatcat gcagtggcgg cggaaggcat cgatggtaaa
      241 gcgataggtt ttctctcccg ctttcacctc ttgcgcttcc agatccacgt cgaaatggat
      301 ccccggattg gctttcacca gcgcaaacag ttcgtccact tctgcatcgc ttaatttcac
      361 cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac tcggcgcaat
      421 caccacttta aagccgtagt cggtcaatgc ccagggcgcg tgctcacgcg aggagccaca
      481 gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcggga agttcagcac
      541 gaagtccggg tttggctgtt ggcctttttc atcgagaaaa cgccagtcgt taaacagatg
      601 cgcgccaaaa cccgtacggg tcactttctg caaaaactgt ttcgggatga ttgcatcggt
      661 atcgacattg gcggcatcca gcggaaccac caggcctgtg tgtttgataa atttctctgc
      721 catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atgtccggtc acagcagcag
      781 cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctgg cggccttcaa
      841 agttacggtt gctggtggat gcacaacgtt cgcccggatt cagacggtcg ttgttcatcg
      901 ccagacacat tgagcagcca ggcaagcgcc attcaaaacc ggcttcaata aagattttat
      961 ccagaccttc cgcttccgcc tgggctttta ccgggccaga gccgggaacc accagtgcct
     1021 gcacgcctgg cgcgactttt cgccctttgg cgatctccgc tgccgcgcgt aaatcttcaa
     1081 tgcgcgagtt ggtacaggaa ccgataaaca ctttgtcgat agccacttcg gtcagcggaa
     1141 tgcccggttt cagccccata taggccagcg ctttttctgc cgacgcgcgt ttaaccggat
     1201 cggcaaacga agccggatcg ggaatattgt cgttcacgga aatcacctgg cctggattgg
     1261 tgccccaggt gacctgcggt gaaatttctt ctgcttgcag agtgacaacg gtatcgaaag
     1321 ttgcgccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg aaatctttgc
     1381 ctttcggcgc atgcaaacgg cctttgacat agttaaaggt ggtttcgtcc ggtgcaacca
     1441 gaccggtttt tgcgcccatt tcgattgcca tattgcacag ggtcatacga ccttccatgc
     1501 ttaaatcacg gattgcttcg ccgcaaaact ccaccacatg cccggtgccg cctgcgctac
     1561 cggttttacc gataattgcc agcacgatat cttttgcggt aatgcccggc gcggctttgc
     1621 cctggacttc aattttcatg gtttttgcgc ggccctgttt cagggtttgc gttgccagta
     1681 cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg ccgtgggtgg
     1741 cggtatgcga gtcgccgcag acaatggtca tccccggcaa ggtgacgccc tgttccggcc
     1801 ccattacgtg gacgatcccc tgatacgggt gattcaggtc atacagttcg acgccaaatt
     1861 ctttgcagtt tttaatcagc tcctgcatct ggatgcgcgc catttcacca caggcattaa
     1921 tgtctttcgt ctgggtagag acgttgtgat ccatagtggc gaaggttttg cccggctgac
     1981 gtaccgggcg accgtgggcg cgcagaccat tgaacgcctg cggtgaggtc acttcatgca
     2041 ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac actacgtgag
     2101 cgtcgaataa tttttcgtat aacgttttag ccatgattac accccttctg ccacatagcg
     2161 ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg ctaaatcccc
     2221 ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgc aagccgcatc
     2281 atcggcatcc aggctgtagc gcagcagcag tgccagcgac agaatttgcg caatcgggtt
     2341 ggcgatgttt ttgcctgcga tatctggtgc tgagccgcct gccggttcat acagaccaaa
     2401 accttgctcg ttcaggctgg cggaaggcaa catccccatc gagccagtga tcattgcgca
     2461 ctcgtcagac agaatgtcgc caaacaggtt ggagcacagc agaacgtcaa actgtgatgg
     2521 atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt cgacatccgg
     2581 gtattccgtg gcgatctcgt tgacgatttc acgccataaa atagaggatt gcagcacgtt
     2641 agctttgtcg attgaggtga ctttgtggcg acgtttacga gcagattcaa acgcgatgcg
     2701 ggcaatacgc tcgatctcaa aacggtgata cacctcggta tcaaacgctt tttcatattg
     2761 tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca gttcgcgcac
     2821 gcacaggatg tcgaagccgt tagcggcaat gtctgcacgc agcggacaga atgcttccag
     2881 cccctgatac agttttgccg ggcgcaggtt gctgaataat ttgaagcgct tacgcagagg
     2941 cagcagcgcg ccgcgttctg gttgctggtc tggtggtaaa tgttcccact tcgggccgcc
     3001 taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg caggcggcag
     3061 tggttgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg tggtgatgcg
     3121 catcgcaaag cggttgcgca cggcattcag cactttcagc gcctgggtca tcacttccgg
     3181 accaataccg tcccccggca atacggcaat atggtaattc ttcgacatca cacggtttcc
     3241 ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc tgcggcacgc
     3301 cagatattgt tcagtacgtg caccatggct ttggcggagg actcgacaat atcggtggcc
     3361 aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac ctgacccagc
     3421 gcatctttac cgtgaccttt ggcggtcagg ctgtatttca ccagttcgac gttatagtca
     3481 gtgatgcggt taattgcctg atagacggca tcgaccggac cgttaccgtt ggcggcttct
     3541 gctttgactt cttcgccaca ggccagtttg acggcggcgg tggcgatatc gttagagcca
     3601 gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg cttaccgatg
     3661 aaggccagcg cctccagatc gtaatcaaac acctgacctt ttttgtccgc cagcttcagg
     3721 aaagcatcgt acaaattgtc taaattatat tcactttctt tataccccat ctcatccatg
     3781 cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat ttggttcaga
     3841 ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagcac gccatcctgg
     3901 tggataccgg aggagtgagc gaatgcgctg ctgccaacaa tggctttgtt tgccgggatc
     3961 ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg gtgattaatg
     4021 gcggtgtgga cgttgagaat atccttacga actttaatcg ccatgatgac ttcttccagc
     4081 gaacagttac cggcacgctc gccgatcccg ttcattgcac cttccacctg gcgtgcaccg
     4141 gcatgtaccg ccgccagtga gttgccgacc gccaggccta aatcgtcgtg ggtatgtacg
     4201 gaaataatgg ctttgtcgat gttaggtacg cgttcataca ggccgctgat gattccggcg
     4261 aactcaaacg gcatggtgta gcccacggtg tccggaatgt tgatggtggt ggcaccggca
     4321 ttaatcgccg cttcgaccac tcgcgccaga tcggcaatgg gtgtacgccc ggcatcttcg
     4381 caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata gatagcgcgt
     4441 tcgatcacct cgtccagggt gctgcgcagc ttggtggcga tgtgcattgg ggaggtagca
     4501 ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggccgc cacgtcgata
     4561 tctttttcca cgcagcgagc taacgcacat acgcggctgt ttttaacctg gcgggcgatg
     4621 gtttgcaccg attcaaaatc gcccggcgaa gagacgggga aaccgacttc catcacgtca
     4681 acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact caagcttgcc
     4741 tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg ctggctcatg
     4801 gtttaggtcc ttgtctcttt tagagcgcct cgcttcgggc ataaaaaaac ccgcgcaatg
     4861 gcgcgggttt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc actcgtctac
     4921 cgcgcaaaga agatgcgttt agtagtagta gaccgataaa gcgaacgatg tgagtcatta
     4981 aatcagctcc agatgaatgc gatatgcttt tagagttact ggatacaaaa acggatgtca
     5041 accctgacgc aataaaaacg tcccgccagc gtgagttctg catccgtaaa attagctaat
     5101 tgtgctgcgg tggttaaagt aagcgatatt aatttctgct taactaccga cgcttttcat
     5161 cggttgacat atttcagcat aaatttttgc atctaatcaa cgaggaaaaa ggggacaaaa
     5221 tgcacgcgtt gcaaaaccta tcctgatgat ttgtattgaa ttatatgttt tgcgattttt
//
LOCUS       DAZOYL010000260         5243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZOYL010000260 DAZOYL010000000
VERSION     DAZOYL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.261"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            204..551
                     /locus_tag="ACHUOY_003387"
                     /pseudo
     CDS             204..551
                     /locus_tag="ACHUOY_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            604..999
                     /locus_tag="ACHUOY_003388"
     CDS             604..999
                     /locus_tag="ACHUOY_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR0684075.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1040..1783
                     /gene="cmoA"
                     /locus_tag="ACHUOY_003389"
     CDS             1040..1783
                     /gene="cmoA"
                     /locus_tag="ACHUOY_003389"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR0684076.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1780..2751
                     /gene="cmoB"
                     /locus_tag="ACHUOY_003390"
     CDS             1780..2751
                     /gene="cmoB"
                     /locus_tag="ACHUOY_003390"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR0684077.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2916..>5243)
                     /gene="torZ"
                     /locus_tag="ACHUOY_003391"
     CDS             complement(2916..>5243)
                     /gene="torZ"
                     /locus_tag="ACHUOY_003391"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR0684078.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1180 a         1362 c         1279 g         1422 t
ORIGIN      
        1 gactataccg atactgcata gaatttgtgt aggattgcat agatacttgg aaaaacaccg
       61 ttaaattgca tagtcattca cctgatattg tcctgttttt acaggtagtt gattcggatg
      121 ttctaggaat catcctcata gttgattcgg ttagttgggc ttgtaccaac ccctaatcaa
      181 cctcatagtg tatttagacc ttgatgattt tagacagccg ccctgttcat gcagcacgtc
      241 cacacagtga agctattcgc gacgctcaac gaaaaaaacc taaagttccg gtacatgctg
      301 tactgacggc gacaaatccg ctgattcgtt ttatcggtag tgatgatatg acgcaaaacc
      361 gggaattatt tcaggtctgg ttacagaaat tagcgcagtg gcatcagacc actacgcctt
      421 atcttttttt acatacgcca gacattgccc aggccccgga actggtacat accctgtggg
      481 aagacttacg taaaacgctt ccagagatcg gagcagttcc ggctattcca cagcaatctt
      541 ctcttttctg aatttgccac ctatcataga caggtgccat cggccatttt aaagggagtt
      601 tgtatggtaa gcgcgctata tgccgtttta agtgcgttgt tattaatgaa gttctctttt
      661 gatgtcgttc gcctgcgaat gcagtaccgc gttgcctatg gtgacggtgg ttttagcgaa
      721 ctgcaaagcg ctattcgcat tcatggtaac gcggtggaat atattcccat cgcgattgtg
      781 ctgatgctgt ttatggaaat gaatggcgca gaaacctgga tggtgcatat ttgcggcatc
      841 gttttgcttg ctggtcgtct gatgcattat tacggttttc atcaccgtct gtttcgctgg
      901 cgacgttccg gcatgagcgc cacctggtgt gcgctgttgc tgatggtgct ggcgaatctt
      961 tggtatatgc cctgggagtt ggttttctcc ctgcgttagc gcacaatacg ccactttctt
     1021 tttcccggat ttttacgtta tgtctcaccg cgacacgcta ttttctgccc ctatcgccag
     1081 actgggcgac tggacctttg atgaacgggt agctgaagtc ttcccggata tgatccagcg
     1141 ttccgttccc ggctattcca atattatttc catgattggt atgttagccg agcgcttcgt
     1201 tcaacctggt acgcaggttt acgatctggg ttgttctctg ggcgcggcga cgctctcggt
     1261 gcgtcgcaac attcatcatg ataattgcaa aattattgcc atcgacaact ccccggcgat
     1321 gattgaacgc tgcagtcgtc atattgacgc ctataaagcc cctacgccag tagacgtcat
     1381 tgaaggtgat attcgcgata tcgccattga aaacgcatcg atggtggtgc tgaattttac
     1441 cctgcaattc ctggaacctt ccgagcgcca ggcattactg gataaaattt atcaaggact
     1501 gaacccgggc ggcgcgctgg tgctttcgga aaaattcagt ttcgaagatg ccaaagttgg
     1561 tgaactgttg ttcaacatgc accacgactt taaacgtgcc aacggttaca gcgaactgga
     1621 gatcagccag aagcgcagca tgctggaaaa cgtgatgctg accgattccg tggaaaccca
     1681 taaagcacgc ctgcataaag ccggttttga gcatagcgag ctgtggttcc agtgctttaa
     1741 ctttggttca ctggtggcat taaaagcaga ggacgctgca tgatcgactt tggtaacttt
     1801 tattctctga ttgccaaaaa tcatctttca cactggctcg aaacgctgcc cgcgcagatt
     1861 gctaactggc agcgcgagca gcagcacggg ctgtttaagc agtggtccaa cgcggtggaa
     1921 tttctgcctg aaattaaacc gtatcgtctg gatttattgc atagcgtaac cgccgaaagc
     1981 gaagagccac tgagcaccgg gcaaattaaa cgcattgaaa cgcttatgcg gaacctgatg
     2041 ccatggcgca aagggccgtt ctcactgtat ggcgtcaaca tcgataccga atggcgttcc
     2101 gactggaaat gggatcgtgt tctgccccat ctttctgatt taaccggacg caccattctg
     2161 gatgtcggct gcggcagcgg ttatcacatg tggcgcatga ttggcgcagg cgcgcacctc
     2221 gcggtgggga tcgaccctac gcaactgttt ctgtgccagt ttgaagcggt gcgtaaacta
     2281 ctgggtaacg atcagcgcgc acatttgtta ccgttaggta ttgaacagct tccggcactg
     2341 aaagcctttg ataccgtctt ttcgatgggc gtgctttacc atcgccgttc gccgctggag
     2401 catctctggc agttgaaaga tcaactggtg aatgagggtg aactggtgct ggaaacgctg
     2461 gttattgatg gcgatgagaa cacggtgctg gtaccaggcg atcgttacgc gcaaatgcgt
     2521 aatgtctatt tcatcccttc cgcgctggcg ctgaaaaact ggctgaagaa gtgtggtttt
     2581 gtagatatcc gcattgtgga tgtgtgcgtt accactactg aagagcagcg acgcactgaa
     2641 tggatggtta ccgaatctct gtccgatttc ctcgacccac atgatccgag taagacagtt
     2701 gaaggttatc ctgcgcctaa acgcgcggtg ctgattgcgc gcaagccgta aaggtctggt
     2761 attactgccg gatgcggcgt gaatgcctta tccggcctac aaacccttgc taattcaatc
     2821 tattgcaggg actatgtagg cctgataagc atagcgcatc aggcagtttt acgtttgcat
     2881 aacctcagcg cccgtttccg ggcgctattc acttcttacg cctgaacagc aggcttatca
     2941 aatgccgtta acttcggtgc gttacccgta tatttttcga tatacaccag cgcagagtta
     3001 ccggcacagg catttgccag ctgcgagctg gggatatccg ccgttaacac gttcgcactg
     3061 ccgtttttac acaagccgtt ttccagatct ggccatgcac cttcatgcac gcataccacg
     3121 ccttttttga tcccgtcagt caccaccgcg cctgtcaaaa tctgaccacg tttgttccag
     3181 acacgcacca gatcgccatt cgcaatacta aatcgagcag catcttcggt gtgaatagtg
     3241 atcggttcac gatcggcaac cgcatatttt ttacgcagtt ccgcatagtt aagctgactg
     3301 tgtaaacggt gtgccggatg tgcggtcaga agctgcaact gcttctcgtc ggcggtaccc
     3361 ttccactcat caggcgcaag ccaggttggg tgtgccgggc aatccttata gccaaatttt
     3421 tccagcgttt tggagtaaat ctcaattttg ccacttggcg tacccagcgc atttttcacc
     3481 ggatcggcgc ggaaatcacc ataacgaacg tactgttcgt tcttctcgct gcggcgcatt
     3541 tcgatcagtt tattttgctg ccagaaggca ttaaacattg gcatagtgac gcgttgcgca
     3601 cgggcccctt tctgagcggc atcatagaaa aatttcagcc acgccatttc atctttacct
     3661 tcggtataga tctcttttcc gccaggtttg agtaattccg caagatcggc aaacacatca
     3721 aaatcgtttc gcgcttcaaa ttgcggagca acagcctgct tcatcggcac aatatgctgg
     3781 ttgctgtaat caccggtcat cgtcaagtca ttgcgctcaa acgatgtggt gatcggtaat
     3841 acgatatctg cgtgtttagc agctgcggtc cagtagcatt cagaaacgac gatcatctcc
     3901 ggtttctgcc atgctttaat cagacgatta gtatcctggt gatgggtaaa gttaccgcca
     3961 cccgcccacc agatcatttt gatattcgga tacgtttgct ctttaccgtt atgctgatat
     4021 ttgccgccag gattttccag cgcatcgacg atacgggcaa caggaatcgc cctcattccg
     4081 ccatcatcag cggcctcgct ggcctgtccg gcaatcgccg cagacatttc cggcagcacg
     4141 ccgccaacac gtgtcgggtt gccaccattg gagtaatgat aagagaaacc aaaaccgccg
     4201 cccggtgtac caatctgccc taacatcgcc gccagcgtca ccagcatcca gtgtttctgt
     4261 tcaccatatt gctggcgctg aattccccag cctgccatca gcattgtgcg attcgccgcc
     4321 atcagttcag cgagtttgac tatctgcgct tccggtacgc cagtaatttc cgccgcccag
     4381 gccgcgctct tcggcgtatt atcgctctta cctctcagat actcttcaaa ctgcggatac
     4441 ccggtagtgt atttttcaag gaacacttta tcgtgtttgc cttgtgtcat cagggtatgc
     4501 gcaatcccta acatcagtgc cacgtcggtg cccatattcg gcgcgatcca ggtggcatta
     4561 tcgccaaaga agtcgatggt ttcggagcgg ataggatcaa tggcaatcac tggtttgcca
     4621 gattttttca gctgatggaa gtattccagc ccttgctcat cggtactgct ccaggcaatt
     4681 tttaaggtat tcagcgggtt catcccccac agcaccacaa cctggctgtt ttccagaatc
     4741 agcggccagg aggtctgctg ttcatacacc tctacagaac cgaccacatg cggcatgatc
     4801 acctgtgccg ccccggttga ataatcgccg ctatgcccgg aataaccgcc cgccaggttc
     4861 atataacgtt gcagtaaggt ttgcgcttta tgcaacacgc cagaagagcg ccagccgtat
     4921 gatccggcaa aaatagccga tggtccgtta gctttgcgaa tgcgatcgtg ttgctcatga
     4981 atcagtttta atgcttgttc ccagctcacc tgtacatagg tatcttcgcc acgacctttc
     5041 gtcggttgca gtggattatc gagatagctt tttctcacca tcggatgctg aatacgcgtc
     5101 gtggtgtgta cctgatccgc cgccgtagac tgtaaggaat tcggtatggt tttcgccagc
     5161 gcgcctgttg aagaaacaat cttgccgtcc ttcacttcta cgttcatcgc tccccaacgt
     5221 tccgcggtga ggattttacc gtc
//
LOCUS       DAZOYL010000261         5218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZOYL010000261 DAZOYL010000000
VERSION     DAZOYL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.262"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..2769)
                     /locus_tag="ACHUOY_003392"
     CDS             complement(<1..2769)
                     /locus_tag="ACHUOY_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0684079.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGV"
     gene            complement(2826..5198)
                     /locus_tag="ACHUOY_003393"
     CDS             complement(2826..5198)
                     /locus_tag="ACHUOY_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0684080.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1294 a         1219 c         1193 g         1512 t
ORIGIN      
        1 tactccagta taagtattta ccggatgaga aagatattgt ttaacggcag tgttaacatt
       61 ctctaccgtc atttgtttca acaattgctc ctgctcagtc catgctgcag gatcgtcata
      121 ttgaataaga ctatttacaa tagtgttcgc taattgttga acgctacgct gttggatatc
      181 gaggctgcgc tgaacgtttt gctggtattc attcagttct tgctcactga tccctttagc
      241 cagacgctta accatcactt cattcgctaa cgttaacagt tcatcatgtc gttctggttg
      301 acaagtaaaa gccagcaaat gactgatatc tttggcctga ggatcaaccg agaggcgaga
      361 agaaacgctg tatgctccag atgcctgttc acgaatatta acgcgtagat cttttgccag
      421 tgcgacgtta aaagcatcga gcgccatacg cgtcgccaga ttaacaggtg tccgggaatc
      481 ataacgcttc cactgtgaaa cctgtgccac aggttcattt tgttctttta cagtaaccga
      541 tgcgttgtcc gtcgcgcgag ttaatggttt acctgcggct aatggcgaat cagagtgttt
      601 gattgatcct aagtaacgcg taattaacgc cacgagtttg tcttctgaga cattaccgac
      661 aatgacaaac gtgatatccg ctggagatga aaacaactgg cgatcggcag ccagcgcatc
      721 tgcggcagta aactgtacaa tctgattttc ttgcagtaat ttcgtgcggt catcagcata
      781 gcgcgtctca tacatctgct gggcgaattt ctccgctgga cgctggtcga gcgttttcaa
      841 cgcctgaatt tgagcatttt gtagcgatgc ccaaatattg tcgttaatcg tgctgtgggt
      901 gattcgctgg ttaatcaact gaaaaccagg ttcagggtta ttagtccgcg cgctaacgga
      961 gagcaacgta ttcatgccac tgactttact gctcatggtt accgaatttt ccgcactcca
     1021 gcgtttcagg ctggaagagg agagctcacc aacgccgctt ccgcttactg ctttatttgc
     1081 cagcgcgata agtgattttt gctgcgcagg gaaacttaaa tcgcctttat tagatacggc
     1141 agtaatttgt agcttttgct cttcacccgc ggattttgcc agaataaccc tggcaccatt
     1201 ggaaagtgtt aatgatgtca gattctcagc cagcgtttct ttgctgctaa tttccgcctg
     1261 tgggtcagcg tctactgtta acgataaatt tctgcctggg aagatgtaag ccgccagctt
     1321 tttgttggcg tactcttttt ccagcgccag gatagccgca agagacaatg cttttttggc
     1381 ggcaagctca ttgtttacca tttgctccca aaatgcgtcc tggttctttc ttaactgctg
     1441 ccatttttcc gccagacttt gcacggtaat ttgctgccac agacgtttcg aaagttgata
     1501 tgtctcttcc ggcgacaaga acggcgtatt atttaatgag ctggatgcca ggcgactggt
     1561 cagcatacgt aaatcacgtt cagcttgctg atcaaccgca tttttcagcc aggtgaggcg
     1621 ggtagatttg acatcatcga gttcttcagc agaaaaacca tgctgatcaa tggttgccaa
     1681 ctctgccatt aatgcatttg cagcatcctg catattatcg tctcgtgcat ttacacggaa
     1741 aaacagcgac tgataatcgg gtgcaatttt aacgctgcgc gcagtgccgc cagaaatagt
     1801 cttcaactcg cccgactgta tgcgttcctg cagacgttga ttgaacagct gaactaacat
     1861 gctccattca gcttgttcga caaagctttg ctcatcgttc acttgtacca ttggcaggcg
     1921 ataatagagt gcgatgccgt tcactcggtt ttctttatca ttaatgatat taaagcgcag
     1981 gtggttttcg gcttttgtcg gccagacgcg attttcagct gctttgttag ccggaagctt
     2041 acttaaatta tcctttatca gcgccagcgc ttctttactg tcgatatcgc cgaccacgat
     2101 aaaggtcata ttatttggtt gataccagcg ttgataaaat tggcgcaatt gtgccggtgt
     2161 gaccgtggcg acagtatcca tcaggccgat aggttcacgg tctaaattac gggtatttgc
     2221 cagcaggaaa gggcggcgcg cctgagaggt gcgccatttc gcatcctgat gggcacgcca
     2281 ttcctcagta attacgccgc gttcagcgtc tacttcgagt atttcaaagg ttgcggcatt
     2341 actccattca ctgaagattg ccatcacttg ttgcagattt tgtttctgcg tagtcggcag
     2401 actcacctga tacagcgttt cgtcatagct ggtataggca ttaacatcgc gaccgaaacg
     2461 caggcccatt gactcaaatg tttcgatgac tttattaccc ggccatgttt ttgtgccgtt
     2521 aaacatcata tgttctacaa aatgagccac gccgcgctca ttgtcttctt cctgcaatga
     2581 accggtatga atttgcagcc ataaatttac ctgatccttt ggttgagcat gcggataaat
     2641 catatatcgc aagccattgt ccagttgccc ggtaattaac ttttcatcct gcggtaaggc
     2701 ggcggcaatc agccgcccgg ggagcaacag agttgccact aacgtcagta agaaacagag
     2761 gtttctcata attatctcca tgcgaaaacc gggcgaattt acccggttaa gtaaaatccg
     2821 aactattaaa atttcatgct gacatcaagc cagaaagtac ggccactggc gtaggtcgcc
     2881 acaccggtgt tcgttgtatc aacagcggtt ttgctatcca gtacattgag aatatcggca
     2941 ctgatcgtca ggttttgttg tttcagaaat tgtggcgtcc aggacaagcg ggtgtcccag
     3001 gtcaggctgc tatccagttt ttcgtcaaca tactgcttgt aatctgaata ttcgctgatg
     3061 tattgcgcat ttgtcttacc aaggataatg cgagctttac gcgcttcttg ccaggccagc
     3121 gtattcgccc acactaaccc gctcggttga tgcgtgaaat ccatgtttaa ggagatcttt
     3181 aatgggttat taaaatctgc cactggaaca ctatcgtaag agaccagatt accgttataa
     3241 accacctggt tatcaccggt attgctctcc tcataaccat tgtttaacga caagttgccc
     3301 ttgctcttga tatagctaaa gacaatttgt gggttaatat ctacttggcg gatatgcagg
     3361 ggttcggcca gttcaaaact gaggctgaac gaatgcgttt tggttttgcc atcgttgtta
     3421 tattcagtaa tggtggtttt agtcgcgctg tcggtacgac tgcttttgct gatttgatca
     3481 tgcgcttcac ggtaaacata gtttgcacgc gcaataacgt tcttaccgat tttctgctgc
     3541 aatcccattg ccagttcatc gttataaggc gttttcaaat cctgataacg cgtcagagtt
     3601 ttattacctg ataccgattc cgtccagcta ttgcggatat cacgtaatcc catatcaaga
     3661 atattcccgc catagtaacg gttataaccg gcggtaatca ttgaggtttg atcagcaaaa
     3721 atatcccatt ccgtcataaa gcgcggggag atattgtggt ttgacagata gttgtcatag
     3781 tcataacgca caccgggcat taacgacaca ttccgccagc taatgtgctc cgccatatac
     3841 agcgtgtagt tgtcaattcc caggctgcct ttacctttat ggtaaatggt atggttagtt
     3901 ttctttccgg cagcattaat cacataggat tcagactggt tatggcgttc agtccatgca
     3961 tcggaataga tgtattccgc gccgaagtag ggttgatgcg aaacatcacc cacggcgaat
     4021 ttttgccagt ccaggcgtgt tttgaaggtg taattatcta cagcctggga aatgtgtcct
     4081 aatccgccac aggtacaacg ccctgtaata tcaccatatg tacatgaaag ttcggtgtac
     4141 cagatgtcat gatcgtgacg ggtataatca ctaatatgat cccaaccaac ggtggtacgt
     4201 agtttggccc atgcgagttg cgtatccata tcccatgcca gaccatagga tttattaccc
     4261 atttcgcgat cagactgcgg gaaggtgctg gtattataat cacggctgga gccggtatat
     4321 tttaaggtta aatcgtgggt aaagcggtcg ctggcaaacc aggtaaattt gctcaatgca
     4381 gtatcgataa cgtttttata ctgtgcccga ccggtgacaa tgccgtcatt cgaaacataa
     4441 tccgcgcggg tgatatcaga ctggcggcgc gataaaccgg cggtaacgcc gaagttatca
     4501 gcgagttcct gattaaacga caaggtataa aagttctttt taaaatctgg ggagtaataa
     4561 gtacttcctg aagagccttg attaaatgcg ctcttgttat tctcatcgat atgcgatgtt
     4621 aaccagtccg aacgcgtagt gcgataaccc aatttcacct tgctatcatc agcgttgaag
     4681 cgtttgatct ttgcatcaat taccccgcca ttgaagcgac caaattcaac cggcacaaaa
     4741 ctgtcataaa gcgtcacatt gtccagtaag ctgacatcaa gataataccc ctgtgacatc
     4801 ccgctaatat tggttgcact actggcatcg gactcattcg ctgggttcag gttattagtt
     4861 gcactaatac cgtcaatcaa ataggcattc tggtagggcg acgcaccgtg aatagagatt
     4921 ttctcagggc gaatatctcc ctggttcaac gaggtacttt gcgttgaatc catgcgtacc
     4981 gcagggttgg ttctcagcag atcgctgata ttgccattcc cggtcggtaa acgctcgatg
     5041 ctttcactgg tgtaatgcgt gttgccgttt acgggtacag ggacaggagc atagaccgtc
     5101 atctcttcaa aaaaatgcat gtacatgttt ttgtcatcga cggcctgcgc cacatcagcg
     5161 ccacataaaa tgacgccagg aataagaact cgcttcattt atttatatct ccgtacag
//
LOCUS       DAZOYL010000262         5175 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZOYL010000262 DAZOYL010000000
VERSION     DAZOYL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.263"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..239)
                     /locus_tag="ACHUOY_003394"
     CDS             complement(<1..239)
                     /locus_tag="ACHUOY_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR0684081.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILN"
     gene            complement(236..1906)
                     /gene="menD"
                     /locus_tag="ACHUOY_003395"
     CDS             complement(236..1906)
                     /gene="menD"
                     /locus_tag="ACHUOY_003395"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR0684082.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(1995..3290)
                     /gene="menF"
                     /locus_tag="ACHUOY_003396"
     CDS             complement(1995..3290)
                     /gene="menF"
                     /locus_tag="ACHUOY_003396"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR0684083.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3369..3674)
                     /gene="elaB"
                     /locus_tag="ACHUOY_003397"
     CDS             complement(3369..3674)
                     /gene="elaB"
                     /locus_tag="ACHUOY_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR0684084.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3729..4172)
                     /locus_tag="ACHUOY_003398"
     CDS             complement(3729..4172)
                     /locus_tag="ACHUOY_003398"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0684085.1"
                     /translation="MHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDLTGDNRH
                     ILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDTCTHHWP
                     DKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4255..5172
                     /gene="rbn"
                     /locus_tag="ACHUOY_003399"
     CDS             4255..5172
                     /gene="rbn"
                     /locus_tag="ACHUOY_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR0684086.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         1204 a         1439 c         1318 g         1214 t
ORIGIN      
        1 aagttaagga tgttgtaact aaccagggtt ttacgcagta agtcggtgac atcatcaaat
       61 ccatcgacgc taatcgccgc cgaaccaccg tgacctggga gataaacata caaccgtgag
      121 tagtcggcaa acgcctcgcc cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc
      181 aaaaacacca gccagggtaa acctggtttg ccgtgttttg cctgcgcgtg caggatcata
      241 aatggcttac ctgcgccaga agttgctgga gcgtttgcgc accatcggtg tcgttaacca
      301 ccatttcaat taccgtggtg gttggcgtgc gccaggcatc ggcaagtgcc gtttcaagtt
      361 cctgccagtt ttgcggacga tgatatttca gctcgaacat cgcggcggcg tgctcaaaat
      421 ggacgttttg cggcatcaga tagaaacgct cgcgctcgct tttcggcgtt ggcagcagcg
      481 agaaaatttg cccgccgttg ttgttcacca caattaatat cagcggcgcg gaaacctgac
      541 gcaataacgc cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca atcgccagcg
      601 tcggtttgcc gcttgcccgc tgaacgccgg cggcagtcga gagcagcccg tcgataccgc
      661 tggccccacg gttgctgtac accgggtaac ctgccggaag ttgcgaaagc gcatcaatca
      721 gacgtaccac caggctgtta ccgacaaaca attgcccctg ttcaggcaga tagtcgctga
      781 tgcgatgcgc cagttgcgct tcgccaaacg catcgcgacg ggcaataacc gcctgcattg
      841 cctgttccgc caggcgcggg atttcaacgc accagggctg gcgtttttct gccggatgca
      901 gctccagcca gtcggcaata ttggcaatta agcggcgtcc acgatggtgc gccggatcaa
      961 gccgcccttc gatgtcatcc acaatccagt actcttcggg ttcacagctt gcctgccatt
     1021 gcaggagccg tttgcccgtc aggctgcttc ccagttgcac cacaatttgc gcctgttgca
     1081 gttcgctggt cgctttggca ttgcctaacc agagatcggc acacggcagc ggctgtccgg
     1141 tttgcgacag cacgtcgcca atcagcggcc agccaagagt ttgcgcccac agtgcaactt
     1201 ttttgccctc ttccgcactc atgcgcccgg caaccaccac gccgcgtttt tgtcgccaga
     1261 agaaccagtc gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc cacggtttgt
     1321 cgtcctgcca ccagtcgccc agccgctgtt gccagctaag cccggtgtcg tccatttcgc
     1381 catacagcgg ttcggcaaac gggcagttga tatggactcc accagaatga agcgtaccga
     1441 gagcatggtc gatggtagaa accagccaac gtgcggggat atcctgggtc gggcgcggca
     1501 atgaaatact gtgcgtgggg tgagaggcga acattcccgg ctgacgaatc gcctgattcg
     1561 cgccgcagtc aatcagctcc ggcgggcgat cggcggttaa caggatcagt ttttctccgg
     1621 ttaacccagc ttcaatcagt gccggataga gatttgctac cgccgtaccg gaggtcacaa
     1681 tcaccgccac cggctgcttg ctgactttcg ccagccccag cgccagatgc cccaacccac
     1741 gctcatcgaa atgggtgtgg tgaatgaatg cggaattctc cgccgccgct aacgttaacg
     1801 gtgtagaacg cgagccaggg gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt
     1861 ccagaatgac cgccgcccag cgtcggttaa atgcgcttac tgacatgagt ttgtccggta
     1921 tcaatattgc ggctaagtat aaggagctac aaaaatagat tattgatatg aatcggtaat
     1981 gatgcgactc attactattc catctgtaat aaagtacgca gccctgccgc tttgttgtcg
     2041 atttcctgcc actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc agcatataat
     2101 cgcacgacat tgccgctaat ttttgctgag cgcagggaaa cgcagaattc gctttgctgt
     2161 aatgagagat agcccgcaga accggcgtac cattctcggg tgaacggttc gtgacgggcg
     2221 ataaactgtc gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga
     2281 tgtaaacaga tcacattatc cgctttgttg agtgaagtcc agatacagcg gcgaagatgc
     2341 tgcactttac gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc
     2401 tgtaaccgtt ggcagatatc ttccaccacc agcatattct cgcgctggtt tttatcatcc
     2461 gccatcagcc actcacctaa ctgctgcgcc tgcttatcat caggatgatt tgctaccgtt
     2521 cccgccagcg cttcagtacg cagcgctttg tcacgccgcc gccataaccg ttccggtgaa
     2581 gagccaagaa aagcattttc gccatcaaag gccatgtaaa aatggtagca attcagattc
     2641 agtcgacgac tggcagccat catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca
     2701 gttgcccgag cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt
     2761 tcgattaatt gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa
     2821 tgtaacccag gcaagggctt gatactcacc agtgtggcga taaattctct tgccttaatc
     2881 gcatcgtgct gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca
     2941 cagcgtcgcc attccaggcg tggtaaaagt aaattgccct gcgacgggtc aaacgcattc
     3001 agcccccaaa tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg
     3061 tccaacgacg taaaacgggt aatcgcgccc aggacggcag cttcttcatc accattacgt
     3121 tgttgccagt agaattgcgg gtatgtttgc tgactggcca gccagctcaa ggcatcaaaa
     3181 gcgtctttga gagggaaagg aatatcgata acccgaatgc cgggtgtcgc cggaatctct
     3241 tgcgacaaat ggcgcagtag attttccagc gccgtagtaa gtgattgcac gcggacctct
     3301 ccctgttaaa agcctcacat tatacggggt actacaaaaa aaatgcagta ccccggtgta
     3361 gggaggtttt aacggcgtgc cagcaacagt cctagtacca gcccaacggc cgcgcccaca
     3421 ccaattcctt gccagggttt ttcgtggacg tagtcatcag cacgataaac tgcctgcttc
     3481 gcccgatagt aataactgtc tgaagcctgg ctaacccgtt ttttcacatc atccagcgct
     3541 ttttctgcac gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg
     3601 agcacctctt ccagtgtttc actaagcagc gtcaggtcgt catcgatacg tgtatcacca
     3661 aactgattag acattctcgt tctccatttg cgtaaaacct gtcactaact atagacaagg
     3721 gttgttgatt acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat accatcttct
     3781 tcatagacct ccgtcacagg gataaaaccg aaactctggt agaagttttg cagatgcgcc
     3841 tgcgccccga ggtaaacagg cttatcaggc cagtgatgcg tgcaggtgtc gagggtttta
     3901 ctcatcagtt gctgccccac tttttcgcca cgcaacgctt cactgacaat cacccgacca
     3961 ataacgaccg gctcaagatc atcatcactt ttcagaatcc tcgcatatgc caccagttca
     4021 tcatttttcc aaccgagaat atggcgatta tcccccgtca aatcatcacc atcgatatcc
     4081 tgataagggc aattctgctc gacaacaaac accgcgcaac gcagttgtaa taaggcataa
     4141 agctgggaaa ccgataattc agagtgatgc agatcttacc attcaatcat aatgctctcc
     4201 ttttagatgt atgccttata ctagccggaa tattttttga aacgtgatga actcatggaa
     4261 ttaatttttt taggtacttc agccggtgtg ccaacccgca cgcgcaatgt cacggcaata
     4321 ttgcttaacc tgcaacatcc gacccagagc ggactttggt tgtttgactg cggtgaaggc
     4381 acccagcatc agctactgca taccgccttt aaccctggga aactggacaa gatttttatc
     4441 agtcaccttc atggcgatca tctctttggt ttacccggct tgctgtgcag tcgttctatg
     4501 tcaggcatta tccaaccctt aacgatttat ggtccgcaag gtatccgtga atttgtggaa
     4561 accgcgctgc ggattagcgg ctcatggacc gattatccgc tggaaattgt cgaaattggc
     4621 gctggcgaaa ttctcgatga tggcctgcgc aaagtgaccg cttatccgat ggaacaccca
     4681 ctggaatgtt atggctatcg tattgaagaa catgataaac cgggtgcatt aaatgcccag
     4741 gcattaaaag ctgctggcgt gccgcctggc ccactgtttc aggaattaaa agcgggcaaa
     4801 acaatcacgc tggaagatgg aaggcagatt aacggcgcag attacttagc tgctccagtg
     4861 ccaggtaaag cgctcgctat tttcggcgat accggcccct gcgatgccgc acttgacctg
     4921 gctaaaggtg tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa
     4981 gccaatagtc gcggccatag ctctacacgc caggctgcga cactagcccg tgaggctgga
     5041 gtcggcaagc taatcattac ccacgtcagc tcgcgctatg atgacaaagg ttgtcaacac
     5101 ctgttacgtg aatgcaggtc aattttcccg gcgactgaac tggcgaatga tttcaccgtg
     5161 tttaacgttt aactc
//
LOCUS       DAZOYL010000263         5153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZOYL010000263 DAZOYL010000000
VERSION     DAZOYL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.264"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            73..735
                     /gene="yqjA"
                     /locus_tag="ACHUOY_003400"
     CDS             73..735
                     /gene="yqjA"
                     /locus_tag="ACHUOY_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR0684087.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            739..1122
                     /gene="mzrA"
                     /locus_tag="ACHUOY_003401"
     CDS             739..1122
                     /gene="mzrA"
                     /locus_tag="ACHUOY_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR0684088.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            1269..1637
                     /gene="yqjC"
                     /locus_tag="ACHUOY_003402"
     CDS             1269..1637
                     /gene="yqjC"
                     /locus_tag="ACHUOY_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR0684089.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            1675..1980
                     /locus_tag="ACHUOY_003403"
     CDS             1675..1980
                     /locus_tag="ACHUOY_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR0684090.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            1983..2387
                     /locus_tag="ACHUOY_003404"
     CDS             1983..2387
                     /locus_tag="ACHUOY_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR0684091.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            2377..2676
                     /locus_tag="ACHUOY_003405"
     CDS             2377..2676
                     /locus_tag="ACHUOY_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR0684092.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            2862..3254
                     /locus_tag="ACHUOY_003406"
     CDS             2862..3254
                     /locus_tag="ACHUOY_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0684093.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            3324..4309
                     /gene="yqjG"
                     /locus_tag="ACHUOY_003407"
                     /pseudo
     CDS             3324..4309
                     /gene="yqjG"
                     /locus_tag="ACHUOY_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            4602..4967
                     /locus_tag="ACHUOY_003408"
     CDS             4602..4967
                     /locus_tag="ACHUOY_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0684094.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1282 a         1253 c         1297 g         1321 t
ORIGIN      
        1 aacagcaata acattgcggt agtgcatctt ttaaaaccag cgtggcgtta accgattcac
       61 caggaataat gaatggaact tttgacccaa ttgctgcaag ccctgtgggc gcaggatttt
      121 gaaaccctgg ccaatccatc gatgattggc atgttgtatt ttgtcttgtt tgtaattttg
      181 ttccttgaaa acggcttgct tccagcggcc tttttaccgg gcgacagttt actggtattg
      241 gtcggcgtgt tgattgcgaa aggcgcgatg ggttacccgc aaacgattct gctgctgacc
      301 gttgccgcca gcctcggctg ctgggtcagc tatattcagg ggcgatggct gggcaatacc
      361 cgcaccgtac aaaactggct atctcattta cccgcgcatt atcatcaacg cgcacaccat
      421 ctttttcata aacacggttt atcggcgctg ttaattggtc gctttattgc gtttgtcaga
      481 acactgctgc cgacgattgc cgggttatca gggctgaata acgcgcgctt tcagtttttc
      541 aactggatga gcggtctgct gtgggtattg atcctgacaa ctctgggtta catgctcggc
      601 aaaacgccga tatttttaaa gtacgaggac cagctgatgt catgcctgat gctgctcccg
      661 gtggtgctgc tggtgtttgg cctggcaggt tctctggtcg tgttatggaa aaagaaatat
      721 ggaaatcggg ggtaagggat gcaaatacct cgcatgtcgc ttcgccagct agcctggtcc
      781 ggcactgttt tacttctggt cggcacgctg ttgctggcct ggtccgcggt tcgccagcaa
      841 gagtctacgc tggcgattcg tgccgttcat caaggcacaa cgatgccaga cggtttttcg
      901 atctggcatc accttgacgc tcatggcatt cctttcaaaa gtatcacccc caaaaacgac
      961 actctgttaa ttacatttga ctccagcgac cagagcgccg ccgcaaaagc ggtcctcgac
     1021 agaacattgc cccatggcta catcattgcg cagcaggaca ataacagtca ggctatgcag
     1081 tggctgaccc ggttacggga taactctcat cgcttcggat aacttcctgt tatccgaaac
     1141 atatcactca ctttggtgat ttcaccgtaa ctgtctatga ttaatgaggc ggtggttaac
     1201 actaccgctt cgttgtctgg acaatcgttc ctttgtaata ggtccgaatc acaatggaag
     1261 gttcaagaat gaaataccgc atcgctttag ctgtttctct ctttgctctt agtgccggta
     1321 gttatgccac taccctgtgt caggaaaagg agcaaaatat ccttaaggag atcagctatg
     1381 ccgaaaaaca ccaaaaccag aatcgtattg acggtctgaa taaagccctg agtgaagtcc
     1441 gggccaactg ttcagatagc cagctgcgtg ccgatcatca caagaaaatc gcaaagcaga
     1501 aagatgaggt ggcggaacgc cagcaagatt tagccgaggc gaagcaaaaa ggcgatgccg
     1561 ataagattgc caaacgcgaa cggaaactgg cagaagcgca ggaagagctg aaaaagctgg
     1621 aagcgcgcga ctactaactc acaatagtca ctacttactc acctggagaa aactatgtcg
     1681 aaagaacaca ctacggaaca tctgcgtgct gagttgaaat ccctttccga tacgctggaa
     1741 gaggtgctta gctcatctgg cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa
     1801 gcggagcagg cactgaaaca gagccgttat cgcctgggtg aaaccggtga tgccatcgcc
     1861 aaacaaaccc gtgtcgcggc ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc
     1921 ggcgtgggaa ttggcgctgc aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa
     1981 ttatggcgga cactcatcac gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc
     2041 gaattgtttc catcatggtt gaaatggtgg aaacacgtct gcggctggcg gtggtggagc
     2101 tggaagagga aaaagctaat ctctttcaac ttttactgat gctgggcctg acgatgcttt
     2161 tcgctgcatt tggtcttatg agcctgatgg tgctaattat ttgggcggtt gatccgcaat
     2221 atcgcctgaa tgcgatgatt gccaccaccg tggtgttgct gctactggca ctgattggcg
     2281 gtatctggac gttacgtaaa tcgcgtaaat ccaccttgct gcgccataca cgccatgagc
     2341 tggcaaacga tcggcaactg ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa
     2401 cgacgtaagg cgcaactgct tagccagatc cagcaacaac ggctggatct ttccgccagt
     2461 cgtcgtgaat ggctggaggc aacaggcgct tacgatcgtc gctggaatat gctgctaagt
     2521 ctgcgctcct gggcgctggt tggcagtagc gtgatggcga tctggacgat tcgccatcct
     2581 aatatgctgg tccgctgggc cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt
     2641 aaaacgaccc tcaagcagca acagcttcgc ggttaacttc cctctggccg gagcaattcc
     2701 ggccttatcc ctcaaatttt ttgaagattt ttgacagttt tccttgctaa caatcatcat
     2761 tcaccacgtt tatgattctc tccatcgaca gcaacgacgc taataccgcg cccttgcaca
     2821 aaaaaacaat cagcagcctg agtggcgcag tggagagtat gatgaaaaaa ttagaagatg
     2881 ttggtgtact ggtagcgcgc attttaatgc cgattctgtt tattaccgca ggctggggaa
     2941 aaatcaccgc ttactctggt acccaacaat atatggaagc aatgggtgtc ccgggtttta
     3001 tgctgccgct ggtgattctg cttgagttcg gtggtggtct ggcaatcctg ttcggtttcc
     3061 tgactcgcac cacagccctg tttactgcgg gctttacgct gctgacggca tttttatttc
     3121 acagcaactt tgctgaaggc gtcaactcgc tgatgttcat gaaaaacctg acaatttctg
     3181 gcggattcct gctgctggca attaccggtc cgggcgcgta tagcatcgac cgcctgctga
     3241 ataaaaagtg gtaagcacgc gctatactta acgataaaaa acgaggagga agctcctcgt
     3301 ttttgctatt ggaggagaga aaaatgggtc aactgattga cggcgtctgg catgacacct
     3361 ggtacgatac caaatctacc ggcggtaaat ttcaacgttc agcttccgca tttcgtaact
     3421 ggctcactgc cgatggcgca cctggcccca ctggcacagg cggttttatc gcagagaaag
     3481 atcgttatca tctctatatt tcactcgcct gcccgtgggc gcaccgcacg ctgatcatgc
     3541 gcaaactcaa aggactggaa ccgtttattt ccgtttccgt agtgaacccg ctgatgctgg
     3601 aaaacggctg gacctttgat gacagttttc cgggagcaac cggagatacg ctctatcaac
     3661 atgaatttct gtatcagctt tatctccacg ccgatccaca ctacagcgga cgagttactg
     3721 ttcccgtact gtgggacaaa aagaaccaca ccatcgtcag caacgaatca acagaaatca
     3781 tccgcatgtt caataccgcg tttgatgcgc tgggcgcgaa agcgggtgat tactacccac
     3841 cagccctgca aacgaaaatt gacgaactta acggctggat ttatgacacc gttaacaacg
     3901 gcgtgtataa agctggtttt gccaccagcc agcaagccta cgacgaggcg gtggcgaaag
     3961 tgtttgaatc gctggcgcga ctggagcaga ttttaggcca gcatcgttac ctgaccggca
     4021 accagctaac cgaagccgat attcgcctgt ggactacgct ggtgcgtttt gatccagtgt
     4081 atgtgaccca cttcaagtgt gataagcacc gcatcagcga ttacctgaat ctgtatggct
     4141 tcctgcgcga tatctaccag atgccgggga tcgccgaaac tgtcaatttc gatcacatcc
     4201 gtaatcatta cttccgcagc cataagacca tcaaccctac ggggattatt tcaattggtc
     4261 cgtggcagga tctcgatgaa ccgcaggacg agatgttcgc ttcggttaaa aaaaggcacc
     4321 ttacgaagtg cctctgataa ttagctaagt gcatgttgtt catatcggat gcggcgtgaa
     4381 cgccttatca aacctacaaa acatttatat tcaatatgtt gcaatgaccg cgcaggccag
     4441 ataagcgtag cgcatctggc gattttgcgc ttgtcatcag tcttagcgcg ccttcacagc
     4501 actttattaa ttccttgaac gaatatttac tgccatttca ttcacgtcta ttcttaattt
     4561 gctgctttaa aaacaagtga ttgagcaaca ttgaggcaaa aatggactgg tatctgaaag
     4621 taataaaaaa ttatgtcggt ttccggggtc gtgcgcggcg caaagagtac tggatgttta
     4681 ttctggtcaa catcatcttt acgttcgtgc tggggctgct ggataaaatg ttaggctggc
     4741 aacgcgctgg tggcgaaggc atcctgacga caatctacgg tattctggtg tttttaccgt
     4801 ggtgggcagt tcagttccgc cgcctgcacg acaccgaccg ctcggcgtgg tgggcactgc
     4861 tattcttaat cccgtttatc ggctggctta ttatcatcgt ctttaactgc caggcgggta
     4921 cgccaggcga aaaccgcttt ggacctgatc caaaactgga accttaaaac aaatctgctc
     4981 gccggatgtc tgactcatcc ggcttttatc atcatgttga tgaatgaata atttactaat
     5041 gtttatttaa aatatttcaa tgtcgttata taaattacct ataaaaaata accatgtatt
     5101 tatcttaaat atcaaaacct ttataacatc cgaaaaagaa agatacgaat agc
//
LOCUS       DAZOYL010000264         5131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZOYL010000264 DAZOYL010000000
VERSION     DAZOYL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.265"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            9..776
                     /gene="hdhA"
                     /locus_tag="ACHUOY_003409"
     CDS             9..776
                     /gene="hdhA"
                     /locus_tag="ACHUOY_003409"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR0684095.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            997..1587
                     /gene="uidR"
                     /locus_tag="ACHUOY_003410"
     CDS             997..1587
                     /gene="uidR"
                     /locus_tag="ACHUOY_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR0684096.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1976..3787
                     /gene="uidA"
                     /locus_tag="ACHUOY_003411"
     CDS             1976..3787
                     /gene="uidA"
                     /locus_tag="ACHUOY_003411"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR0684097.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3784..>5131
                     /gene="uidB"
                     /locus_tag="ACHUOY_003412"
     CDS             3784..>5131
                     /gene="uidB"
                     /locus_tag="ACHUOY_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR0684098.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGFCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQ"
BASE COUNT         1250 a         1218 c         1354 g         1309 t
ORIGIN      
        1 gacggaaaat gcgccatgcg ccatgcgcca tgcgccatgc gccatgcgcc atcatcacag
       61 gtgcgggtgc aggtattggt aaagaaatcg ccattacatt cgcgacagct ggcgcatctg
      121 tggtggtcag tgatattaac gccgacgcag ctaaccatgt tgtagacgaa attcaacaac
      181 tgggtggtca ggcatttgcc tgccgttgtg atattacttc cgaacaggaa ctctctgcac
      241 tggcagactt tgctatcagt aagctgggta aagttgatat tctggttaac aacgccggtg
      301 gcggtggacc taaaccgttt gatatgccaa tggcggattt tcgccgtgct tatgaactga
      361 atgtgttttc ttttttccat ctgtcacaac ttgttgcgcc agaaatggaa aaaaatggcg
      421 gtggcgttat tctgaccatc acttctatgg cggcagaaaa taaaaatata aacatgactt
      481 cctatgcatc atctaaagct gcagccagtc atctggtcag aaatatggcg tttgacctgg
      541 gtgaaaaaaa tattcgggta aatggcattg cgccgggggc aatattaacc gatgccctga
      601 aatccgttat tacaccagaa attgaacaaa aaatgttaca gcacacgccg atcagacgtc
      661 tgggccaacc gcaagatatt gctaacgcag cgctgttcct ttgctcgcct gctgcgagct
      721 gggtaagcgg acaaattctc accgtctccg gtggtggggt acaggagctc aattaataca
      781 ctaacggacc ggtaaacaac cgtgcgtgtt gtttaccggg ataaactcat caacgtctct
      841 gctaaataac tggcagccaa atcacggcta ttggttaacc aatttcagag tgaaaagtat
      901 acgaatagag tgtgccttcg cactattcaa cagcaatgat aggcgctcac ctgacaacgc
      961 ggtaaactag ttattcacgc taactataat ggtttaatga tggataacat gcagactgaa
     1021 gcacaaccga cacggacccg gatcctcaat gctgccagag agattttttc agaaaatgga
     1081 tttcacagtg cctcgatgaa agccatctgt aaatcttgcg ccattagtcc cgggacgctc
     1141 tatcaccatt tcatctccaa agaagccttg attcaggcga ttatcttaca ggaccaggag
     1201 agggcgctgg ctcgtttccg ggaaccgatt aaagggattc attttgttga ctatatggtc
     1261 gagtctattg tctctctcac ccatgaagcc tttgggcaac gggcgctggt ggtcgaaatt
     1321 atggcggaag ggatgcgtaa cccacaggtc gccgccatgc ttaaaaataa gcatatgacg
     1381 atcacggaat ttgttgccca gcggatgcgt gatgcccagc aaaaaggcga gataagccca
     1441 gacatcaaca cgtcaatgac ttcacgttta ctgctggatc tgacctacgg tgtactggcc
     1501 gatatcgaag cggaagatct ggcgcgtgaa gcgtcgtttg ctcagggatt acgcgcgatg
     1561 attggcggta tcttaaccgc atcctgatcc tctcatttcg cgggctggtg ataactgtgc
     1621 ccgcgtttca tactttaatt tccctgtaca aaatacaatc tagccgggat cggagaattc
     1681 ccccccaaaa atattcaccg tagccatatg tcatgagagt ttatcgttcc caatatgctc
     1741 gaacgaacgt tcggttgctt attttatggc ttctgtcaac gccgttttaa agattaatgc
     1801 gatcaatatc acgctgtggg tattgcagtt tttggttttt tgatcgcggt gtcagttctt
     1861 tttatttcca tttctcttcc atgggtttct cacagataac tgtgtgcaac acagaattgg
     1921 ttaactaatc agattaaagg ttgaccagta tttttatctt aatgaggagt cccttatgtt
     1981 acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag
     2041 tctggatcgc gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag
     2101 ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa
     2161 ttatgcgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca
     2221 gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca
     2281 ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt
     2341 tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac
     2401 tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca
     2461 tgatttcttt aactatgccg ggatccatcg cagcgtaatg ctctacacca cgccgaacac
     2521 ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt
     2581 tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca
     2641 ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg
     2701 gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg
     2761 tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct
     2821 gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttgcg
     2881 tggcaaagga ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg
     2941 ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga
     3001 tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg
     3061 cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg
     3121 ggaaactcag caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca
     3181 cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg
     3241 ggaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac
     3301 ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt
     3361 gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga
     3421 gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat
     3481 caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag
     3541 tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc
     3601 cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg
     3661 cgttggcggt aacaagaagg ggatcttcac ccgcgaccgc aaaccgaagt cggcggcttt
     3721 tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa
     3781 acaatgaatc aacaactctc ctggcgcacc atcgtcggct acagcctcgg tgacgtcgcc
     3841 aataacttcg ccttcgcaat gggggtgctc ttcctgttga gttactacac cgacgtcgct
     3901 ggcgtcggtg ccgctgcggc gggcaccatg ctgttactgg tgcgggtatt cgatgccttc
     3961 gccgacgtct ttgccggacg agtggtggac agtgtgaata cccgctgggg aaaattccgc
     4021 ccgtttttac tcttcggtac tgcgccgtta atgatcttca gcgtgctggt attctgggta
     4081 ccgaccgact ggagccatag ccgcaaagta gtgtatgcat atttgaccta catgggcctc
     4141 gggttttgct acagcctggt gaatattcct tatggttcac ttgctaccgc gatgacccaa
     4201 caaccacaat cccgcgcccg tctgggcgcg gctcgtggga ttgccgcttc attgaccttt
     4261 gtctgcctgg catttctgat agggccgagc attaagaact ccagcccgga agagatggtg
     4321 tcggtatacc atttctggac gattgtgctg gcgattgccg gaatggtgct ttacttcatc
     4381 tgcttcaaat cgacgcgtga gaatgtggta cgtatcgttg cgcagccgtc attgaagatc
     4441 agtttgcaaa ccctgaaacg gaatcgcccg ctgtttatgt tgtgcatcgg tgcgctgtgt
     4501 gtgctgattt cgacctttgc ggtcagcgcc tcgtcgttgt tctacgtgcg ctatgtgtta
     4561 aatgataccg ggctgttcac tgtgctggta ctggtgcaaa acctggttgg tactgtggca
     4621 tcggcaccgc ttgtgccggg gatggtcgcg aggatcggta aaaagaatac cttcctgatt
     4681 ggcgctttgc tgggaacctg cggttatctg ctattcttct gggtttccgt ctggtcactg
     4741 ccggtggcgt tggttgcgtt ggccatcgct tcaattggtc agggcgttac tatgacagtg
     4801 atgtgggcac tggaagctga taccgtagaa tacggtgaat acctgaccgg cgtgcgaatt
     4861 gaagggctca cctattcact attctcattt acccgtaaat gcggtcaggc aatcggaggt
     4921 tcaattcctg cctttatttt ggggttaagc ggatatattg ccaatcaggt gcaaacgccg
     4981 gaagttatta tgggcatccg cacatcaatt gccttagtac cttgcggatt tatgctactg
     5041 gcattcgtta ttatctggtt ttatccgctt acggataaaa aattcaaaga aatcgtggtt
     5101 gaaattgata atcgtaaaaa agtgcagcag c
//
LOCUS       DAZOYL010000265         5114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZOYL010000265 DAZOYL010000000
VERSION     DAZOYL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.266"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..379
                     /locus_tag="ACHUOY_003413"
     CDS             <1..379
                     /locus_tag="ACHUOY_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684099.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="ACHUOY_003414"
     CDS             complement(575..2125)
                     /locus_tag="ACHUOY_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR0684100.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="ACHUOY_003415"
     CDS             2297..2743
                     /locus_tag="ACHUOY_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR0684101.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="ACHUOY_003416"
     CDS             complement(2937..3872)
                     /locus_tag="ACHUOY_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0684102.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4393..>5114
                     /locus_tag="ACHUOY_003417"
     CDS             4393..>5114
                     /locus_tag="ACHUOY_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684103.1"
                     /translation="MNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMTVGK
                     AFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSIAQN
                     NNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYLDRM
                     LYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKNASP
                     LITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAZOYL010000266         5069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZOYL010000266 DAZOYL010000000
VERSION     DAZOYL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.267"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..1135)
                     /locus_tag="ACHUOY_003418"
                     /pseudo
     CDS             complement(1..1135)
                     /locus_tag="ACHUOY_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1135..1317)
                     /gene="ymfR"
                     /locus_tag="ACHUOY_003419"
     CDS             complement(1135..1317)
                     /gene="ymfR"
                     /locus_tag="ACHUOY_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR0684104.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1329..3062)
                     /locus_tag="ACHUOY_003420"
     CDS             complement(1329..3062)
                     /locus_tag="ACHUOY_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR0684105.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3059..3553)
                     /locus_tag="ACHUOY_003421"
     CDS             complement(3059..3553)
                     /locus_tag="ACHUOY_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR0684106.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3675..4025)
                     /locus_tag="ACHUOY_003422"
     CDS             complement(3675..4025)
                     /locus_tag="ACHUOY_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0684107.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4068..4532)
                     /locus_tag="ACHUOY_003423"
     CDS             complement(4068..4532)
                     /locus_tag="ACHUOY_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0684108.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4529..5026)
                     /gene="rrrD"
                     /locus_tag="ACHUOY_003424"
     CDS             complement(4529..5026)
                     /gene="rrrD"
                     /locus_tag="ACHUOY_003424"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR0684109.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
BASE COUNT         1098 a         1484 c         1262 g         1225 t
ORIGIN      
        1 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tggaagagta aattccccag
       61 ttgatcccgg tggcgtaggc ttcaaaacgg gacttcatat ccccgcgcag taacgccccg
      121 gcgttaaatt tggcgtaata aacgccctgc ttactttttc gtaccagtcc ggtgttgatc
      181 cgctgttcga tgcgggtcag atacggcacc agtgaatagt tgataaatcc cagccccagc
      241 tcttcgatat tgttgaaggt ggcgcgatcg gtgttctgca ccatgtgcaa cggcacccgg
      301 aacagacgac agatttcttc cagctgaaac ttgcgggttt ccaggaactg actgtcctcg
      361 gcgttcagcg ccatcgactt ccagtccagc cccatttcca gaatcatcgg gcggtgagca
      421 ttgccaagcc ccgtgtgaca ctcctcaaaa tctttcttca gacgctcgta agcatccggc
      481 gtgagctttt gttccgtacg caacacaccg gatgtcaccg caccattact aaacaacctg
      541 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cacgcgcata cgcaatggga
      601 ttcagcccga caagtccatc cagcgtcagg gggcgcacat gccagatttc attctgggtc
      661 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
      721 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccaccacttc tcccagtgcc
      781 tttaccttgt aggcgtaaaa attcccccgt agacacaggc agacaataac cagctcccag
      841 aattcctgcg gtgtcatgta gccattaggt tttgccgaaa ccaacttatg cagtcgttca
      901 tccaccgccc gtgttttcag ggtaccgctg attttgtaga gactgcaggg cagcatacca
      961 acagactctg ccagcaccct gacgcaagaa tagaccgccg tcaggcgcat ggcccgctgg
     1021 ctactgatcc gctttccggt ataggtgtcg tatgacagcc cgatagcatc agcagttctg
     1081 ctggcgtggt caccggcaca tcgctttttc gttgaaataa tcccgaaaag aacactattt
     1141 acctccgccg acagactgcc gtgtacggtc gagatatcgc gccaccagcc acgaccagaa
     1201 caggcacagc gccccggcaa caacaaaacc cgccggggga taaatcagcc aggcaccata
     1261 cgccagcaaa agcgcaccca gcacgcccac cagaggcgcg agaatcagca tgatcataat
     1321 tacctcagtt aaagcgagcg gatcccgtag gactcaatgt ggtcagacag cgtgtcttct
     1381 ttctcgtaca gcatggctct gccaaccgcc ataatcagcg caactgcacc atcgattttg
     1441 ttttccgcct gctctttgac gggtttcacc acatcatcgt tacccggcat gtttttgccg
     1501 accacgttgc cgatacacca ggtcatgatg ggattgccgt catgatgaaa acgtcccgat
     1561 tcaatcgctg cttccagctc tttcatcgga tcggacatat tggtgaagtt ctggacgata
     1621 gtgacgggat tcaggtcttc atcagcaagg tcatgtgaca acccggtcgc cccgaagggg
     1681 tcgatgggtg actcactgac cgggctgatt ttgttcgccg ctttggcctc ctcgaggatg
     1741 tagcgataat ccacctccgc accatcggta acggtcagaa cgcccatttc cacccatttc
     1801 tgaaagcgtt cggctgtccg tcgatcttca tttttctcga cgctgtacac cgtgtcatac
     1861 ggtacccaga aacgcggggc cacactgtag taatgcgttt taccgtcaat ctcgcgggta
     1921 taaagtcgcg ccatgctgtt catatccagc ttacgcgcca ggtcaaaggc cagaatgcac
     1981 ggctgcccct cgaactgctc aagggtcagt gatttatcct cgcagctctg ccagctcacc
     2041 aagttgaaat acgccgaacg cgccgacacc cagatattga ggtgttttgt tttaaagacg
     2101 tttgccagac gggcgttatt tttcgcacgc tgctgctgac ttaacaaaaa ttcgcgataa
     2161 accgacacgc caatatttgg attggctttt tccagcacct gcgggccggt ccagtcgtca
     2221 ccttcatcaa cggtatagat gatcccgaac agttcatcgt taggcaccga gccgttgagc
     2281 atctcgatga cttcccgccg cttgtcgtag cacggcccct caatgttgta cccggcggtg
     2341 gtgatggccc acatcagtgg ctgacgtcgc gcccccatcc cggtaagcat tgtggtataa
     2401 agcgcatcgg tggcatgctc gtgatattca tcaaccacgg cacagtgggg tgatgaacca
     2461 tcacctgggt tgccgatcag cggttcaaac cgcgcgccat cctccggacg gttcatgttt
     2521 gaggcgttaa cctcaatccc gaacgcttcc gtcagcatgg gtgtgcgttt acacatcagt
     2581 cgcgccgggc gaaagacttc ccacgcctgt ttctctgtcg tggcaccgga atacacttcc
     2641 gcgccaaact cgttatcaca ggcaaaacaa tacagggcaa caccggcaga gattgctgat
     2701 ttgccgttct tacgggggat ttcggtgtac acctcccgga agcggcgcaa ccgggtgcct
     2761 ttattgaccc agccaaacgc acagcagatc acaaatagct gccacggctc cagcgtgatg
     2821 ggcatccgtt tgaatgccca ctcccccttg gtgtgcggca acagctgaat aaatttcgcg
     2881 gcccgttcag ccaggtcctt gtcgaagcgg taacgaaacg acttactttt ttccgccatc
     2941 aggtcatcaa gatggcgctg gcaggcctga atcacaaact ggcaggcaac aatctttccg
     3001 cgcacgacat cccgggcata ctgatttgca gcatttacgt tggggtaaga tttccggctc
     3061 atgattcgat aattttcaga aacgggttag tggctttctt ctgccccgcc aggccaatca
     3121 gacgctggcg gctgctgggg tcgagtccga gcattgcccc cgtgctgctc atctcggact
     3181 cctgttcttt tttggcggtc agctccggat ttttgaccct gccgcccatt gcaccggtga
     3241 tggtgttgcc ctgtatggca atatttttca cggcacgtcg ccagaactca taggccacgc
     3301 accaccgctc aagtaccgcc aggtcagtca cgcacagcag gccctgaccg cagagttctt
     3361 tggttgtcag ttgccacatg atcgtggcga gagggagatc ttcttcagcg aaccactctg
     3421 gtggctcaac acctttgatg ggcgtaaaaa caggttcatc tttgttcagg gctcgcttgc
     3481 cggggtttcc ggccagcgcc ttgcgcgccg ttggcttggg gcgacgcccg gaacgccccg
     3541 ccgttccagc catatgcggc actcctggtt aaatttcatt tttcgcgggt ataaaaaaac
     3601 gatggggcgg gcagtccgga agacgtcagg tcacagggat ttgccccgcc cctccctaca
     3661 agtgagaata attatcaccg gattcgttcg cgcgctgttt tcgctttatg gcagggccag
     3721 cacagactct gcaggttgct gtctgcgtct gttccgccat gcgctttcgg gatgatgtgg
     3781 tcgacggttt tcgcctcgct caccacaccg acacgcagac acaactgaca cagaccttta
     3841 tcgcgcttca gaatacgggc acgaatcacc gtccattttg agccatagcc acgctggtgg
     3901 cggctcagtc cgcgctggtg ctgcacccag ccttcaccgc gatgtttatc gcagtaaccc
     3961 gaactgtctg tggttgtacc tgcacatcca cgcttacggc aggcacgtgg gattagtgat
     4021 ggcataaata cctcataccc tgcgaaatgt ttaccacgat aaaaaggcta cttaatgcac
     4081 tgagtgcgga tatactcctg tgccccttcc agttgcatct gcatcgtcat cagccgctct
     4141 ctgagggtga aataatcccg ttcagcggtg tctgccagtc gggggctggt tgcattatcc
     4201 acgccggagg cggtggtggc ttcacgcact gactgacaga ctgctttgat gtgcaaccga
     4261 cgacgaccag cggcaacatc agcgcgcaga gtttcatttt cagctttcgc atcagctaac
     4321 tccttcgtgt atttagcatc gagtgcatta acatcacgct gtctctgctg catgtcagta
     4381 atggttgcgt tcgccagctt cagttctctt gcgtttttgt cgcgctgctc tttgtaggta
     4441 atggcgttat cacggtaatg attaacagcc catgacaggc agacgatgat gcagataacc
     4501 agagcggaga taatcgcggt gactctgctc attgctgccc ccacaaacag acttcacgct
     4561 caatctcacg acgggtcatc agccctttcc attgcttacc gccagcatat gtccagcgac
     4621 gtagctggtc acatgcgcct ttgatatcgc cctggtttat tttgcgaaga agcgtcgatg
     4681 ttctgaaatt gccagcgccc acgttgtaaa cgaacgagta aagagcgccg cgcgttgttt
     4741 ccggtatatc gactttgatg tacgggttaa tttgtctggc gacagtggca aggtctttat
     4801 tcaggagggc tttgcattct gcttcggtat acgttttacc gagcatgatg tcttttccgg
     4861 tgtgtccgtg acatacagtc catacaccaa tgatatcttt gtatggtatg tagctgacac
     4921 cttccagacc atcgttacca cctgggccag tgattaacac agatgctata gcaacagccc
     4981 caccaccaat agcagctgca acagccttgc gtaatgatgg cgacattatt cacctctcgc
     5041 agccttacgc ttgtcttctc ttattttga
//
LOCUS       DAZOYL010000267         5044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZOYL010000267 DAZOYL010000000
VERSION     DAZOYL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.268"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="ACHUOY_003425"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="ACHUOY_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1771
                     /locus_tag="ACHUOY_003426"
                     /pseudo
     CDS             1595..1771
                     /locus_tag="ACHUOY_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1779..1868)
                     /locus_tag="ACHUOY_003427"
                     /pseudo
     CDS             complement(1779..1868)
                     /locus_tag="ACHUOY_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHUOY_003428"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHUOY_003428"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR0684110.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="ACHUOY_003429"
     CDS             complement(3750..4700)
                     /locus_tag="ACHUOY_003429"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0684111.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="ACHUOY_003430"
     CDS             complement(4839..>5044)
                     /locus_tag="ACHUOY_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR0684112.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1230 g         1210 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAZOYL010000268         5026 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZOYL010000268 DAZOYL010000000
VERSION     DAZOYL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.269"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..619
                     /locus_tag="ACHUOY_003431"
     CDS             <1..619
                     /locus_tag="ACHUOY_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFR0684113.1"
                     /translation="QKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQ
                     IETVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSL
                     RVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLN
                     GTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            687..4682
                     /locus_tag="ACHUOY_003432"
                     /pseudo
     CDS             687..4682
                     /locus_tag="ACHUOY_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1199 a         1262 c         1632 g          933 t
ORIGIN      
        1 gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga taaacaacca
       61 tgctgaaacc attggcaaca atcagatgat tgcggttacc aacaatcaga tagagacggt
      121 gggcgttaac cagatagaga cggtgggcag taaccagata attaaagtgg gttctgttca
      181 ggtggaaacg attggacttg ttcgtgcgct gacggtgggc gtggcgtatc agacgacggt
      241 aggtggcatt atgaacacct cggtggcact gatgcagtcc tcgcagatcg gtttgcataa
      301 atcgttgagg gtcgggctgg gttatgacgt caaagtcgga aataacgtta ccttcaccgt
      361 tggtaaaacg aaaaaggatg ataccgggca gaccgcgatt tactccgccg gtgagcatct
      421 ggagctctgc tgtggtaagg caaggctggt gctgacgaag gacggacaaa tttttctcaa
      481 cggcacaaaa attcatttgc agggtaagga gcaggttaat ggtgactcac tgttgattaa
      541 ctggaactgt gcagcctcga aatccccacc gaagacccct gatgaaaagc aggatacgcc
      601 ggatatgaga gagtactgat cccgtacctg ttcggtattc agtaacaaac cagcattaaa
      661 aataaacaga agaaggaatt tgaatcatga gcggaaaacc agcggcgcgt cagggagata
      721 tgactcagta tggcggtccc attgtccagg gttcggcagg tgtaagaatt ggcgcgccca
      781 ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg gtaaatccgc
      841 tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc ccgctgccgt
      901 tcattctctc ccgcacctac agcagctacc ggacgaagac gcctgcaccg gtgggcgttt
      961 tcggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgtgat gacggactga
     1021 tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg ggggaggcgg
     1081 tgtacagccg cagtgagtca atgtggctgg tgcgcggtgg taaggcagca cagccggacg
     1141 gccatacgct ggcgcggctg tggggggcgc tgcgccggat atccggttaa gcccgcatct
     1201 ttacctggcg accaacagcg cacaggggcc gtggtggata ctgggggggg ctgagcgggt
     1261 gccgggtgct gaggacgtac tgccagcgcc gctgccgccg taccgggtgc ttaccgggat
     1321 ggcggacgct gacgtaccgg cgtgaggccg ccggtgacct ggccggggaa atcaccgacg
     1381 tgacggacgg tgccgggcgg gagttccgtc tggtgctgac cacgcaggcg cagcgtgcgg
     1441 aagaggcccg cacctcttcg ctatcttctt ctgacagttc ccgccctctc tcagcctcag
     1501 cgttccccga cacactgccc ggtaccgaat acggccccga caggggtatc cgcctttcgg
     1561 cggtgtggtt gatgcacgac ccggcatacc cggagagcct gcccgctgcg ccactggtgc
     1621 ggtacacgta tacggaagcc ggtgaactgc tggcggtata tgaccgcagc aatacgcagg
     1681 tgcgcgcttt cacgtatgac gcgcagcgcc cgggccggat ggtggcgcac cgttacgcgg
     1741 gaaggccgga gatgcgctac cgctacgacg atacggggcg ggtggtggag caactgaacc
     1801 cggcagggtt aagctaccgc tatctttatg agcaggaccg catcaccgtc accgacagcc
     1861 tgaaccggcg tgaggtgctg catacagaag gcggggccgg gctgaaacgg gtggtgaaaa
     1921 aagaactggc ggacggcagc gtcacgcgca gcgggtatga cgcggcagga aggctcacgg
     1981 cgcagacgga cgcggcggga cggcggacag agtacggtct gaatgtggtg tccggcgata
     2041 tcacggacat caccacaccg gacgggcggg agacgaaatt ttactataac gacgggaacc
     2101 agctgacggc ggtggtgtac ccggacgggc tggagagccg ccgggaatat gatgaaccgg
     2161 gcaggctggt atcggagaca tcgcgcagcg gggagacagt acgctaccgc tacgatgacg
     2221 cgcacagtga gttaccggcg acgacaacgg atgcgacggg cagcacccgg cagatgacgt
     2281 ggagccgcta cgggcagttg ctggcgttca ctgactgctc gggctaccag acccgctatg
     2341 aatacgaccg cttcggccag atgacggcgg ttcaccgcga ggaaggtatc agcctttacc
     2401 gccactatga caaccgtggc cggttaacct cggtgaaaga cgcacagggc cgtgaaacgc
     2461 agtatgagta caacgccgca ggcgacctga ctgccgttat caccccggac ggcaaccgga
     2521 gcgagacaca gtacgatgcg tggggaaagg cggtcagcac cacgcagggc gggctgacgc
     2581 gcagtatgga gtacgatgct gccggacgtg tcatcagtct gactaacgag aacggcagtc
     2641 acagcgactt cagctacgat gcgctggacc ggctggtaca gcagggcggc tttgacgggc
     2701 ggacgcaacg ttatcattat gacctgaccg gaaaactcac gcagagtgag gatgaggggc
     2761 ttgtcaccct ctggcactac gatgcgtcgg accgcatcac gcaccggacg gtgaacggcg
     2821 acccggcaga gcagtggcag tatgacggcc acggctggct gagagaaatc agccacctga
     2881 gcgaaggcca ccgtgtcgct gtccactacg gctacgacga taagggccgc ctgacgggcg
     2941 aacggcagac ggtggagaac ccggagacgg gggaactgct gtggcagcat gagacgaaac
     3001 acgcctacaa cgaacagggg ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg
     3061 agtggctgac ctacggcagc ggttacctgg cgggcatgaa gctgggcggg acgccgctgg
     3121 tcgagtatac gcgggacagg ctgcaccgtg agacggtgcg caccctcgca ccggacctgg
     3181 acatccgcat cccgtatgcc accgacccgg cgggaaaccg cctgccggac ccggagctac
     3241 acccggacag cacgctcacg gcgtggccgg ataaccgcat cgcagaggat gcgcactatg
     3301 tttaccacta tgatgaatac ggcagactga cggagaagac ggaccgcatc ccgacgggtg
     3361 tgatacggac ggacgacgag cggacccacc actacgacag ccagcaccgc ctggtgttcc
     3421 acacgcggat acagcatggc gagccactgg tcgagagccg ctacctctac gacccgctgg
     3481 gacggcgaat ggcgaaacgg gtatggcggc gggagcgtga cctgacgggg tggatgtcgc
     3541 tgtcgcgtaa accggaggag acatggtacg gctgggacgg cgacaggctg acgacggtac
     3601 agaccgacac cacacgtatc cagacggtat acgagccggg aagcttcacg ccgctcatcc
     3661 gggtcgagac agagaacggc gagcgggaga aagcgcagcg ccgcagcctg gcggagaagc
     3721 tgcagcagga agggagcgag gacgggcacg gcgtggtatt tccggctgaa ctggtgcggc
     3781 tgctggacag actggaggaa gaaatccggg cagaccgcgt gagcagtgaa agccgggtgt
     3841 ggcttgcgca gtgcgggctg acggtggagc aactggccag acaggtggag ccggaataca
     3901 caccagtgcg aaacgttcat ctttaccact gcgaccatcg gggccagccg ctggcgctca
     3961 tcagcgaaga cggcgatacg gtgtggagcg gggcgtatga tgagtggggc aaccagctaa
     4021 atgaggagaa cccgcatcat gtgtatcagc cataccgtct gccggggcag cagtatgatg
     4081 aggagtcggg gctgtactac aatcgtcacc ggtactatga tccgttgcag agacggtaca
     4141 tcacccagga ccccattggg ctgaaaggtg ggtggaatct gtatcagtat ccgctgaatc
     4201 cggtgagcag gatagatccg ttggggttat tctcatgtga gagttgtagt cattttatag
     4261 atgatataaa taataaactg gataaccttc cgtttaaccc gacaaaaatg tttacagcta
     4321 gtttaaatac tgctaattca gcaaggctgt atgcaagtgg catgacaaaa ttcggtataa
     4381 gtgttgctgc cgatggaacg atcattgctg ccccagtcgg tgcagcttct atggctttag
     4441 gggcgtggaa tataaaatct gcgacagctg catataacag agcaaattta caattatcag
     4501 aatcacttca ggaaaatggt agagataggt gttggagaaa tttattgggt attttacctt
     4561 atggtgagca gtttgatgac cctggtgaac catcaatatt agacgtttat ggtgaaaaga
     4621 ttgagaatat caaagattct ccaatgaagt tttttgaaga gcttgggacg ctggggttgt
     4681 aatgtgtatt tttaatgata tttttatgtt tgtgtttatt gttagtagta tacttgtaac
     4741 cgtgcatttt gtgtattctt tattttttaa aagtggaaag gtaaaagata aggtaaaaaa
     4801 atggattaaa gtaatgtgtg atttattttg ggggatagga tagtggtgtg atatggataa
     4861 atataaatta cagtggctgg catatttcga cctgacgccg gagtagataa aaaaacagac
     4921 aggattggag tatagcaagc gtctgacaat ctctcgggac agtagtatga tgaagaggcg
     4981 gggttgtact ataaccgtaa ccggtactac gatccgctaa agggca
//
LOCUS       DAZOYL010000269         4996 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZOYL010000269 DAZOYL010000000
VERSION     DAZOYL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.270"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHUOY_003433"
     CDS             complement(<1..163)
                     /locus_tag="ACHUOY_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0684114.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUOY_003434"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUOY_003434"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0684115.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUOY_003435"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUOY_003435"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0684116.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUOY_003436"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUOY_003436"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0684117.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUOY_003437"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUOY_003437"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0684118.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUOY_003438"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUOY_003438"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0684119.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
BASE COUNT         1253 a         1293 c         1311 g         1139 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaac
//
LOCUS       DAZOYL010000270         4983 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZOYL010000270 DAZOYL010000000
VERSION     DAZOYL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.271"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            15..1454
                     /gene="norV"
                     /locus_tag="ACHUOY_003439"
     CDS             15..1454
                     /gene="norV"
                     /locus_tag="ACHUOY_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR0684120.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            1451..2584
                     /gene="norW"
                     /locus_tag="ACHUOY_003440"
     CDS             1451..2584
                     /gene="norW"
                     /locus_tag="ACHUOY_003440"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR0684121.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(2712..4964)
                     /gene="hypF"
                     /locus_tag="ACHUOY_003441"
     CDS             complement(2712..4964)
                     /gene="hypF"
                     /locus_tag="ACHUOY_003441"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR0684122.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
BASE COUNT         1188 a         1407 c         1364 g         1024 t
ORIGIN      
        1 aaattgaggt tgctatgtct attgtggtga aaaataacat tcattgggtt ggtcaacgtg
       61 actgggaagt gcgtgatttt cacggtacgg aatataaaac gctacgcggc agcagttaca
      121 acagctacct catccgcgaa gaaaaaaacg tgctgatcga caccgtcgac cataaattca
      181 gccgcgaatt tgtgcagaac ctgcgtaatg aaatcgatct ggcggatatc gattacatcg
      241 tgattaacca tgcagaagag gaccacgctg gggcgctgac cgaactgatg gcgcaaattc
      301 ccgatacgcc gatttactgt actgccaacg ctatcgactc gataaatggt catcatcatc
      361 atccggagtg gaattttaat gtggtgaaaa ctggtgacac gctggatatc ggcaacggca
      421 aacagctcat ttttgtcgaa acgccaatgc tgcactggcc ggacagcatg atgacttacc
      481 tgacaggcga cgcggtgctg ttcagtaacg atgctttcgg tcagcactac tgcgacgagc
      541 atctgttcaa cgatgaagtg gatcagacgg agcttttcga gcagtgccag cgttactacg
      601 ccaatatcct gacgccgttc agccgcctgg taacaccgaa aattaccgag atcctgggct
      661 ttaacttacc agtcgatatg atagcgacct ctcacggcgt ggtatggcgc gataacccga
      721 cgcaaattgt cgagctgtac ctgaaatggg cggctgatta tcaggaagac agaatcacca
      781 ttttctacga caccatgtcg aataacaccc gcatgatggc tgacgctatc gcccagggga
      841 ttgcggaaac cgacccacgc gtggcggtga aaattttcaa cgtcgcccga agcgataaaa
      901 acgaaatcct gaccaatgtc ttccgctcaa aaggcgtgct ggtcggcact tcgacgatga
      961 ataacgtgat gatgccgaaa atcgccggtc tggtggagga gatgaccgga ttacgcttcc
     1021 gtaacaaacg cgccagtgct ttcggctctc acggctggag cggcggtgcg gtggatcgtc
     1081 tttccacgcg cctgcaggat gcgggtttcg aaatgtcgct tagcctgaaa gccaaatggc
     1141 gaccagacca ggacgctctg gagttatgcc gtgaacatgg tcgcgaaatc gcccgtcagt
     1201 gggcgctcgc gccgctgccg cagagcacgg tgaatacggt ggttaaagaa gaaacctctg
     1261 ccaccacgaa gtccgacctc ggcccacgga tgcagtgcag cgtctgccag tggatttacg
     1321 atccggcaaa aggcgagcca atgcaggacg ttgcgccagg aacgccgtgg agtgaagttc
     1381 cggataactt cctctgcccg gaatgctctc tcggcaaaga cgtctttgac gaactggcat
     1441 cggaggcaaa atgagcaacg gcattgtgat cattggttcg ggcttcgccg cccgccaact
     1501 ggtgaaaaat attcgcaaac aggacgccag tattccatta accctgatcg ccgccgacag
     1561 catggatgag tacaacaaac cggacctcag ccatgttatc agccaggggc aacgtgccga
     1621 cgaccttacc cgccagacgg cgggtgaatt tgccgggcag tttaatctgc acctgtttcc
     1681 gcacacctgg gtgacggata tcgatgccga agcccatgtg gtgaaaagcc agaataatca
     1741 gtggcaatac gacaagctgg tgctggcaac tggtgccagt gcctttgtcc cgccagtgcc
     1801 tgggcgtgag ttaatgctga cgttaaatag tcagcaagag tatcgcgcct gtgaaacgca
     1861 actgcgggat gcccgacgcg tgttgattgt cggcggtggt ttgattggta gcgaactggc
     1921 gatggatttt tgtcgtgcag gcaaagcggt cacgctaatc gacaacgctg ccagtattct
     1981 ggcgtcatta atgccaccag aagtaagcag ccgcttgcag catcggttga cggagatggg
     2041 cgttcatctg ctgttgaaat ctcagttgca ggggctggaa aaaacggatt ctggcattct
     2101 ggcaacgctg gaccaccagc gtagtatcga agtggatgcg gtaattgccg ccaccggact
     2161 gcgcccggaa acagccctgg cacgacgcgc cgggctgacg attaatcgcg gcgtttgcgt
     2221 cgatagttat ctgcaaacca gtaatgacga tatttatgcg ctgggcgatt gcgcggaaat
     2281 caacggtcag gtactgccat tcctgcagcc gattcaactt agcgcgatgg tgctggcaaa
     2341 aaatcttctc ggcaataaca cgccgctgaa actaccggcg atgctggtga aaatcaaaac
     2401 gccggaatta ccgctgcatc tggcaggcga aacccagcgt caggatttac gctggcaaat
     2461 taataccgaa cgccagggaa tggtggctcg cggtgttgac gatgctgacc agcttcgcgc
     2521 ctttgtggtc agtgaggatc ggatgaaaga ggcatttgga ttgttgaaaa cattgccgat
     2581 gtaggtgggc tactgtgcct aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta
     2641 cggggacgca tgtgtaggcc agataaggcg tttacgccgc atccggcaat ggtgtccaaa
     2701 tgcaacacgt tttatccgtt ctggacttca cccgctaacc aacgcgccgc agcaataacc
     2761 ccctgcccca gagacaaacc gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg
     2821 aaatcagcga gataatgcgc cagacgtgca cgcagcaaac ggttatgaat aaccccgccg
     2881 ctaaatacca gcgtagtgat accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa
     2941 ccctgcgcca gcgcatcatg aaacgcccac gcgcgttgat taaccggtgc ctgccagttc
     3001 agccactgct gccagaaagc ggcgagatcc agttgattgt ccaccagcgg catcgtcacc
     3061 ggatgcgtca ctccgtggca tgaggctgcg agcgcctcca gagcacaagc cgcttcacct
     3121 tcataactta acgtggctgg cgcacagccc agtgccgccg ccactgcatc gaacaaccgc
     3181 ccacacgatg acgccagcgg cgcgttaatt ccacgctcaa tggcccgcgc cagcacgctc
     3241 cagttttgct gttgcacact tgctgtttca gggtaattct gccactccgg cacaaagcgc
     3301 aggcactgcg ccagcaggtt tcgccacggc tgcttcgctg ccaaatcgcc acccggaagc
     3361 gccactgcag gcaagccgcc caggtgctca cattcgcgat agttcacccg caggcactcg
     3421 ccgccccaca aagcgccgtt ctcccccata ccgataccgt cgagcgtcaa agcaatgaca
     3481 tcaccgccat ccaacggcca ctgatgctct gccagacacg ccgctgcatg ggcatgatga
     3541 tgcagtaccg tttgcgtcgg cagattcatt tcacgcgccc actggctgga gacatagccc
     3601 ggatgcgcgt catgcacaac gtattgcggg gtaaaatcgt agatgttttg catcaggcgt
     3661 aacgcttcgc gccactgcat ctggatgcca tcgtcactta aatcgcccag atgctgactc
     3721 aacaccgctt gttcaccgcg caccaggcag aaggtatttt tcagatccgc gccgagacac
     3781 agcacaggcg gaacattttt aaagcccgga ggcaaagcca gcgcatccgg cacatacccc
     3841 cgcgaacggc gcagcatttc gccgctttcg cgcaccaccg aatcatccat ccgctgcacg
     3901 atgtcgcggt tatgtatcaa gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt
     3961 tcgttgctga tagctggtgg tttaccgctc aggttgccgg aggtcatcac cagcgggcat
     4021 tgcagttcct gtaacagcaa atgctggagc gggttcgcag gcaacattac cccgacttcg
     4081 ttaaggtcag gggcgatatc atcacaaagc tcaggaacgt attttttatc caccagcaca
     4141 atcggcgcgg cgggcgtggt aagcaactgg cgcgcagcgt ctggtaaacc gtcagccact
     4201 ggcaacatga ccgccagcgg tttcgccggg cgatgtttgc gcgcccgaag tgtcgccacc
     4261 gcgttactgt tacgtgcatc gcaggcaaga tgaaatccgc caatcccttt gatggcgaca
     4321 atgttgccca tttttaactg tgcgatagcc gcctgtaatg ccgcctcttg ttctgcatgt
     4381 tcaccatgac ttacccattc aagatgtggg ccacactccg ggcaggccac cggctgggcg
     4441 tggaagcgac gatcgagcgg gtcacggtac tctttgtcac aagccggaca caacggaaac
     4501 gccgccatca cggtaaacgg gcggtcgtaa ggcatggcgc gaataatggt gaaacgcggg
     4561 ccgcagtggg tacagttgat aaacggataa cgataacgcc gttcgcctgg ggtattcatt
     4621 tcggcaaggc aagcagggca agtagcggca tccgggacaa tttgcgtatt catggcacct
     4681 cccgcgctct ggcggatggt gaactcagtg ggcagttgtg accagatgta cggctcacgc
     4741 tcgacgctat caatacgcgc cagcggcggg cagtgctgat gcaattgaac aagtaacgtt
     4801 tccgggtctt cccgcagccg gacttctacg ccatcgccgt cattacagac atcgccgtga
     4861 agatttaatt gctgtgccag ctgccagaca aacggacgaa aaccgacgcc ctgcactttg
     4921 ccacgaatac gcagttggac accgcaagat gtgttttttg ccattgagtt attcccgcca
     4981 tca
//
LOCUS       DAZOYL010000271         4901 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZOYL010000271 DAZOYL010000000
VERSION     DAZOYL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.272"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHUOY_003442"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHUOY_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(796..1248)
                     /locus_tag="ACHUOY_003443"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHUOY_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHUOY_003444"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHUOY_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUOY_003445"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUOY_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR0684123.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHUOY_003446"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHUOY_003446"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHUOY_003447"
     CDS             complement(2927..3859)
                     /locus_tag="ACHUOY_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR0684124.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..>4901
                     /gene="mlaA"
                     /locus_tag="ACHUOY_003448"
     CDS             4153..>4901
                     /gene="mlaA"
                     /locus_tag="ACHUOY_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR0684125.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDS"
BASE COUNT         1173 a         1145 c         1152 g         1431 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 tatcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccc ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt c
//
LOCUS       DAZOYL010000272         4870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZOYL010000272 DAZOYL010000000
VERSION     DAZOYL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.273"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1336
                     /gene="frlA"
                     /locus_tag="ACHUOY_003449"
     CDS             <1..1336
                     /gene="frlA"
                     /locus_tag="ACHUOY_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR0684126.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGELIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1357..2379
                     /gene="frlB"
                     /locus_tag="ACHUOY_003450"
     CDS             1357..2379
                     /gene="frlB"
                     /locus_tag="ACHUOY_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR0684127.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTEC
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2429..3260
                     /gene="frlC"
                     /locus_tag="ACHUOY_003451"
                     /pseudo
     CDS             2429..3260
                     /gene="frlC"
                     /locus_tag="ACHUOY_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            3257..4042
                     /gene="frlD"
                     /locus_tag="ACHUOY_003452"
     CDS             3257..4042
                     /gene="frlD"
                     /locus_tag="ACHUOY_003452"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR0684128.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4142..>4870
                     /locus_tag="ACHUOY_003453"
     CDS             4142..>4870
                     /locus_tag="ACHUOY_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0684129.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
BASE COUNT         1079 a         1314 c         1341 g         1136 t
ORIGIN      
        1 gggaagccag gaactccaac gcaagctcgg attttgggcc gttcttgcaa tcgccgtcgg
       61 gacaaccgtc ggctccggta tttttgtatc tgtgggtgaa gtggcaaaag cagcgggcac
      121 gccgtggctt acggtgctcg cgtttgtcat tggcgagtta attgtgatcc cgcaaatgtg
      181 cgtctatgcg gaactatcca ccgcttatcc ggaaaatggc gcagattatg tttatctgaa
      241 aaatgccgga agccgaccgc tggctttcct ctccggctgg gccagcttct gggccaacga
      301 tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc aatcttggct ttttaacgcc
      361 tatcgatccg ttgctcggta aatttatcgc cgccggatta attatcgcct ttatgttgct
      421 acacctgcgc tccgttgaag gcggcgcagc gtttcagacg ctaattacca tcgccaaaat
      481 tatcccgttc actatcgtca ttggccttgg gatcttctgg tttaaagcgg agaattttgc
      541 cgcccctacc accactgcga ttggcgcaac gggcagcttt atggcgctgc tggcggggat
      601 ctctgccacc agttggtcgt ataccggcat ggcctctatc tgttatatga ccggcgaaat
      661 taaaaacccc ggaaaaacca tgccacgagc gctgattggt tcctgtctgc tggttctggt
      721 gctctacacc ctgctggcgc tggtgatttc cggcctgatg cccttcgaca aactcgccaa
      781 ttctgaaacg ccgatttccg acgccctgac ctggatcccc gcactcggca gcaccgctgg
      841 gatctttgtt gccatcacgg cgatgatcgt cattcttggt tcgctttcca gctgcgtgat
      901 gtaccagccg cggctggaat acgcgatggc gaaagacaac ctgttcttta aatgcttcgg
      961 ccatgtgcat ccgaaataca acacgccgga tgtctccatc atcctgcaag gggcgctggg
     1021 gatcttcttc atcttcgttt ccgatctcac cagcctgctg ggttatttca ccctggtgat
     1081 gtgtttcaaa aataccctca ccttcggctc catcatctgg tgtcgtaaac gcgacgatta
     1141 caaaccgctg tggcgtactc cggctttcgg gctgatgacc accctcgcca ttgcgtcaag
     1201 cctcattctg gtcgcctcaa cctttgtctg ggcaccgatt cccggcctta tctgcgccgt
     1261 catcgttatt gctactggtc tgcctgctta cgccttctgg gcgaagcgta gccgccagct
     1321 caacgctttg tcgtaatgtt atctggagaa aataaaatgt tggatattga taaaagcacc
     1381 gtggactttc tggtcaccga aaatatggtt caggaagtgg aaaaagttct cagccatgac
     1441 gttccgctgg tgcacgccat cgtggaagag atggtgaagc gcgacattga tcgtatttat
     1501 ttcgttgcct gcggctcgcc actcaacgcg gcgcaaacgg cgaaacatct ggcggatcgc
     1561 ttttccgatc ttcaggtcta cgccatttcc ggctgggagt tctgcgataa caccccgtat
     1621 cgcctcgacg atcgttgcgc ggtgattggc gtttctgact acggtaaaac ggaagaggta
     1681 atcaaagcgc tggagctggg ccgggcctgc ggcgcactca ctgcggcgtt caccaaacgc
     1741 gcggacagcc cgattacctc ggcggcggaa tttagcattg attatcaggc cgactgtatc
     1801 tgggaaattc acctgctgct ctgctacagc gtggtgctgg agatgatcac ccgcctcgcg
     1861 ccgaacgcgg aaatcggcaa gatcaaaaac gatctcaagc cgttgccgaa tgcgctcggt
     1921 catctggtac gcacctggga agaaaaaggc cgccagcttg gtgaactggc cagccagtgg
     1981 ccgatgattt ataccgttgc tgcgggtccg ctgcgtccgc tgggttacaa agaaggcatt
     2041 gtaacgctga tggaatttac ctggacgcac ggctgcgtga ttgagagcgg agagttccgc
     2101 catggcccgc tggagattgt cgaaccgggc gttccgttcc tgttcctgct cggcaatgat
     2161 gaaagtcgcc acaccaccga atgcgccatt aactttgtta aacagcgtac tgacaacgtg
     2221 atcgtcatcg attacgccga aatttcgcaa gggctgcacc cgtggctggc accgttcctg
     2281 atgttcgtgc caatggagtg gctctgctac tacctgtcta tttacaaaga tcacaacccg
     2341 gatgaacgcc gctattacgg tggtctggtg gaatattaat ccctctcccg gcccggcaac
     2401 gggccggatt tacgcaagga gttacccgat gaaaacaggt atgtttacct gcggccacca
     2461 gcggctgcct attgaacacg catttcgtga tgcaagcgag ctgggttacg acggcatcga
     2521 aatttggggc ggtcgcccgc acgcgttcgc gccggactta aaagcgggcg gcatcaaaca
     2581 aatcaaggcg ctggcgcaga cgtatcagat gccgattatc ggctatacgc cagaaaccaa
     2641 cggctatccg tataacatga tgctgggcga tgaacatatg cgtcgcgaaa gcctcgacat
     2701 gatcaagctg gcgatggata tggcaaaaga gatgaacgcg ggttatacgc tgatttccgc
     2761 ggcccacgca ggctatctca cgccacctaa tgttatctgg ggacggctgg cagagaacct
     2821 aagcgaactg tgtgagtacg cggaaaacat cggcatggat ctaatcctcg aaccgttaac
     2881 gccgtatgaa tcgaacgtcg tgtgtaatgc taatgatgtg cttcatgcgc tggcgctggt
     2941 gccttcgccg cgcctgttca gcatggtcga catttgcgcg ccgtatgtcc aggcggaacc
     3001 ggtgatgagt tatttcgaca aactgggcga taaattatcg tcatctgcat attgtcgaca
     3061 gcgacggggc cagcgacacg cattacattc ctggagaagg caaaatgccg ctgcgggaac
     3121 tgatgcgcga tattattgag cggggctatg agggttactg tacggtggag ctggtgacga
     3181 tgtatatgaa cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc
     3241 tgccggagga tgagagatga aaaccctggc gacaatcggc gataactgcg tcgatatcta
     3301 cccgcaactg aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac
     3361 tcgctacggc atacagccgg gatgcattac cttggtgggt gacgatgact acggcacaaa
     3421 gctgaagcag gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg
     3481 cgttaccgca caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac
     3541 cgaaggcgtg atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta
     3601 tgacattgtg cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc
     3661 tgcgggcaaa cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac
     3721 actggtgccg catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg
     3781 tctgaagatg aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga
     3841 aaacggcagc attgcctggg atggcgcgca gttctggcgt caggctcctg aaccggtgac
     3901 ggttatcgac accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc
     3961 tgcggggatg acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat
     4021 tcagtatcac ggtgcctggt aggtataacg ttggcgtgag catctccgcg ccaacgtgct
     4081 gttacttgcc ggaaaacgac cctataatcc gtgtaattca ttctttattt cagggtcgat
     4141 tatgtcagct acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct
     4201 gctggatgat atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga
     4261 gctttgtacg caatataacg tcagccgcat tactattcgc aaagctatca gcgacttagt
     4321 ggcagacggc gtactgatcc gctggcaggg caaaggcacc tttgtacaaa gccagaaagt
     4381 tgaaaacgcc ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaaggc
     4441 gacgaaagag aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct
     4501 gaacatcccc ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga
     4561 gccgctgttt attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat
     4621 ttacgtcgaa ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt
     4681 cagcgacaaa aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa
     4741 atgcgaactg ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa
     4801 accggtgcac gtctccgaac tcttctgccg cgccaatcgc atcaccttaa cgattgataa
     4861 taaaagacat
//
LOCUS       DAZOYL010000273         4860 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZOYL010000273 DAZOYL010000000
VERSION     DAZOYL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.274"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(38..414)
                     /gene="rnpB"
                     /locus_tag="ACHUOY_003454"
     ncRNA           complement(38..414)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUOY_003454"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(447..1592)
                     /gene="garK"
                     /locus_tag="ACHUOY_003455"
     CDS             complement(447..1592)
                     /gene="garK"
                     /locus_tag="ACHUOY_003455"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR0684130.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1689..2579)
                     /gene="garR"
                     /locus_tag="ACHUOY_003456"
     CDS             complement(1689..2579)
                     /gene="garR"
                     /locus_tag="ACHUOY_003456"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR0684131.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2609..3379)
                     /gene="garL"
                     /locus_tag="ACHUOY_003457"
     CDS             complement(2609..3379)
                     /gene="garL"
                     /locus_tag="ACHUOY_003457"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR0684132.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3395..4729)
                     /gene="garP"
                     /locus_tag="ACHUOY_003458"
     CDS             complement(3395..4729)
                     /gene="garP"
                     /locus_tag="ACHUOY_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR0684133.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1172 a         1396 c         1162 g         1130 t
ORIGIN      
        1 aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg tgaaactgac cgataagccg
       61 ggttctgtcg tggacagtca ttcatctagg ccagcaatcg ctcactggct caagcagcct
      121 acccgggttc agtacgggcc gtaccttatg aacccctatt tggccttgct ccgggtggag
      181 tttaccgtgc cacggactgt taccagccgc gcggtgcgct cttaccgcac cctttcaccc
      241 ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac tggtcgtggg
      301 tttccccccc aggcgttacc tggcaccctg ccctatggag cccggacttt cctcccctcc
      361 gcccgtctcc cccgaagagg acgacgacga agcggcgact gtctggtcag cttcggcgcg
      421 cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc gcattccaat cgccagtgtc
      481 gcggcgatat tacgtgaagc acggcagata ttgtcataag ccccgcggaa tgcttcgtcc
      541 aacgtaccta tgctggtcaa tacgctgaag accgcatcga tgccatgctg atgtacaacg
      601 ccaacatcat cggtcaggct acccgcaatg ccaatcaccg gtttatggta cttcttcgcc
      661 acgtttgcaa cgccaatcgg taccttgccg tgaatactct ggctgtcaat acgcccttca
      721 ccggtgatca ccagcgtaca atcgtgaata tgttcttcca gattcagcgc cgtagtgacg
      781 atttcaatac cacttttcag ttccgcacca agaaacgcca tcagcgccgc gcccataccg
      841 cccgcagctc ctgcgccggg gacatctttc acatcaacat gcagcgcttt tttaatgaca
      901 tcggcatagt gagagaggtt attgtccagc tcaacaatca tcgcttcact ggctcccttt
      961 tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca gcggattggt gacatcacaa
     1021 gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc aatatcattc
     1081 agagtattaa gactaccgcc gccaaaacca atttcattgc cgttggcgtc gcataatttc
     1141 gcccccagcg cctgtaccat gcctgcgccg ccatcatttg tagcgctgcc gccaatgccg
     1201 ataataatgt ttgttgcact gctctccagc gcctgcagga ttaactcgcc tgtgccgcgt
     1261 gaagtggtta cgagtggatc gcgtttttcc gcaggtacca gctccagccc actggccgcc
     1321 gccatttcaa taaacgcggt tttgccatcg ccggagatcc cccaactggc attcactttc
     1381 tcgcccagcg gccctgtaac ccaggcgtga cgttcagccc cctgggtggc tgcaatcatc
     1441 gcttccaccg ttccttcacc accgtcggca accggaacag aaacatactg tgcatcagga
     1501 aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga taaactttct
     1561 ttataagagt ctggggcgat tacgattttc atacctatgc ctgttaccac atgacgccgg
     1621 agggcgtttc tcttattcgg cctagattcc aggcccggat tgcaatacac catccgggca
     1681 cgacgtcatt aacgagtaac ttcgactttc gccagttttt cgtagtagca cgccagggcg
     1741 ctatgatccg ccgttcctaa accatctgca cgcagtgcct gcatcatctc cataaccgca
     1801 gctgtgagca gcagttgtgc gccgacgccg tgagaagtat ccagcgcatt cgccagatcc
     1861 ttaatatgca gatcaatgcg gaagcccggc ttgaagttgc ggtccatcac catcggcgct
     1921 ttggcatcca gcacggtact gcccgccagc ccaccgcgaa ttgcctgata aaccaggtcc
     1981 gggttaacgc ccgctttggt tgccagcgtt aacgcttctg acatcgcagc aatattcagc
     2041 gccacaatga cctgatttgc cagtttggtg acgttacctg caccgatttc cccggtatgc
     2101 accacggaac ccgccatcgc tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg
     2161 ccgcccacca tcactgacag cgtaccgtcg atggctttcg gttcaccgcc gctcaccgga
     2221 gcatccagca tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg gcttgccagc
     2281 ggtgcgatag aactcatatc gatcaatacc gtacctggct tcgcgccttc aataatgcca
     2341 ttctcaccca gcgccacctc tttcacatga ggggagtttg gcagcatggt tatgatgacg
     2401 tcgcactgtt cagcgatcgc tttagccgta gacgctgttt ctgcacctgc agcaatcacg
     2461 tcagcaatag cttctgggtt acggtcagca accaccagcg agtaacctgc tttcagaagg
     2521 tttttactca ttggtttacc cataatcccc aggccaataa aaccaacttt catagtcata
     2581 tcaaccatct ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc agtttctgag
     2641 tggcagagcg gaagacgcca agatcgctgc cgacagccac aaacgtcgcg ccccattcca
     2701 gataacgacg cgcatcggct tcgaccggcg cgagaatacc gctgggtttg ccgtgcgcgc
     2761 tggcacggtt aaaaatatgc tgaattgctt tttgtacatc cggatgcgag gcattgccga
     2821 gatggcccaa tgccgcagcc agatcgctgg ggccgacgaa gataccgtct acaccttcgg
     2881 tagcggcaat agcatcgacg ttatctacgc cctgctgact ttctatctgg accagaatag
     2941 taatgttctt gttcgactga gcgaaataat ccgccacggt gccaaacata ttggtgcggt
     3001 gagaaacgga gacgccgcga atgccttccg gcgggtaacg agttgatgcc accgcctgct
     3061 ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt atagaaaccg atatccagaa
     3121 gacgcttaat aattaccggc tcgttggtcg gcactcgcac tactggcgcg ctggcgctgc
     3181 ctttcagggc cattaactgc ggaataaacg tggaaatatc gtttggcgca tgttcgccat
     3241 ccagcaccag ccagtcaaac ccagccaaac caagaacttc agtgctaatc gggttagaga
     3301 gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg aatttattcg
     3361 ggaaaacatc gttattcatc gcttatacct ttgcttattt ctgcaattcc atacgtttaa
     3421 tgtcgccaac tacgaagagg tagcagacca tcgccatcag cgctgaacat cccacgaaaa
     3481 ccagtgctgc attgaaggag tgcagttcac ttaccaggta gccaatcacc agtggagtga
     3541 caatggaggc aacattgcca aagacgttaa agacgccgcc acagaggcca acaatctctt
     3601 tcggcgcggt gtcagaaatc accggccagc ccagagcacc aaatcctttg ccaaaaaaag
     3661 ccagcgccat cagcatgacc accagcgtgg tgttgttggt gtagttacat aagatgatgg
     3721 tggaaaccag caacattccc agcacaatcg gtagcttacg tgccagggtc agggataaac
     3781 cgcgtttgat cagataatcc gagaagacac ctcccagcac gccgcccgca aaaccacaca
     3841 gtgccggaat cgaggcgacc agacccactt tcagaatcga catgcctttt tcctgcacca
     3901 gataaatcgg gaaccaggtg aggaagaacc aggtgatggt gttgataaaa tattgtccga
     3961 aaaatacgcc caacatcatg cggttagaga gcaattgctt gatgtaatgc agtttgggac
     4021 cgcttgctgc cgcgctgccc ggctttttgt ggtccatatc gaccaccgcg ccattttcag
     4081 agataaactt cagctcttcc gcagacatac gtgggtgatc tgtcgggtta tgaatcaact
     4141 tgatccacag cgccgtcagc acaaaaccaa tcccccccat aacggtgaag acgtgctccc
     4201 agccccaagc gaaagtcagc cagccaagca gcggcgaaaa gagcgccagc gagaaatatt
     4261 gcgccgagtt aaagatggcg gaggcagtac cacgttcttt cgtcgggaac caggcggcga
     4321 caatacgggc gttcgccggg aatgatggcg cttccgagaa ccccagcata aagcgcataa
     4381 agaacatgga gatccctgcc caggccagcg ggaacatatc aacaaagcct tgcaggaagg
     4441 tgaacagcga ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca aacttatcaa
     4501 gcaaccagcc gccggggatt tgcatcagca agtaggccca gccaaaagca gagaagatgt
     4561 aacccatcga aaccgcactt aactgcaact cttttgccac ttcggtacca gcaatagaca
     4621 gcgttgcacg atcggcgtag ttaacggcgg taacaataaa aataatcagt aatattaaat
     4681 agctggtatg cacgcctttc ttttttacgt caacggtgtc cagaatcatt ttatttacct
     4741 cgggtactta tgctgatttt tattattatg gggaaggtgt tatttatgag ttttatttat
     4801 gccgttacga caatgaactc gggaattagt ataagcagcg ggagaataat aatcattgtg
//
LOCUS       DAZOYL010000274         4825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZOYL010000274 DAZOYL010000000
VERSION     DAZOYL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.275"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(29..988)
                     /gene="nrdF"
                     /locus_tag="ACHUOY_003459"
     CDS             complement(29..988)
                     /gene="nrdF"
                     /locus_tag="ACHUOY_003459"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0684134.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(998..3142)
                     /gene="nrdE"
                     /locus_tag="ACHUOY_003460"
     CDS             complement(998..3142)
                     /gene="nrdE"
                     /locus_tag="ACHUOY_003460"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0684135.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTNEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3115..3525)
                     /gene="nrdI"
                     /locus_tag="ACHUOY_003461"
                     /pseudo
     CDS             complement(3115..3525)
                     /gene="nrdI"
                     /locus_tag="ACHUOY_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(3522..3767)
                     /gene="nrdH"
                     /locus_tag="ACHUOY_003462"
     CDS             complement(3522..3767)
                     /gene="nrdH"
                     /locus_tag="ACHUOY_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR0684136.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4015..4344)
                     /locus_tag="ACHUOY_003463"
     CDS             complement(4015..4344)
                     /locus_tag="ACHUOY_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR0684137.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4496..>4825
                     /locus_tag="ACHUOY_003464"
     CDS             4496..>4825
                     /locus_tag="ACHUOY_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR0684138.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLF"
BASE COUNT         1184 a         1212 c         1220 g         1209 t
ORIGIN      
        1 ggtagtgata tttttgaaaa taacaccctc agaaattcca gtcttcatct tctgtttcaa
       61 ccgctttccc catcacataa gaggagcctg aaccggaaaa aaaatcgtga ttttcatcgg
      121 cattcggcga aagcgcggcg aggattgccg gattcacttc cgccatttct gcgggaaata
      181 aaggttcgta gcccagattc atcaaagcct tattggcgtt gtaacagaga aacgctttca
      241 catcgtcagc ccacggggtt tcggcgtaca gctcatcggt gtattgcagc tcgttgtcgt
      301 agagttccag caacaaatcg aaggcgaaac tcttcaactc ttcacgttgt cccagagata
      361 tcttttccat gtttttctga tatttatagc ctatgtagta accgtggact gcttcatcac
      421 ggataatcag acggatcagg tctgcggtat tggtcagctt tccgcggctg gaaaaataca
      481 tcggcagcca gaaaccggaa tagaacaaaa aagattcaag aaacacactg gcgattttct
      541 ttttcagcgg atcatcaccg cgataatgtt gctgaataat ctgagctttt cgctgcaacg
      601 gtgcgttttc ttcactccag gcgtaggcgg catcgacatc tttggtctgg cacagcgtcg
      661 agaaaatcga actgtaagag cgggcatgaa ccgcttccat aaagctgata ttcgataata
      721 ccgcttcttc atgaggcgtg agtgcatcgg gcatcagcga aggcgcgccg ataacatttt
      781 gcagcgtgtc gagcagcgtc aggccagtaa aaacgcgcat cgtcagttgt tgttctacga
      841 cagttaatgt ctgccaggca ggaatatcgt tcgacagcgg caccttttct ggtagccaga
      901 aattgctggt caggcgattc cacacctcca gatctttatc gtcagatatc ttgttccagt
      961 tgatggcgct gatacgtgag agtttcatag atattcctta aagtgcacag gagacgcagc
     1021 cttcaatttc agtgccttcc agcgccatct gacgcaggcg aatgtaatag agcgttttga
     1081 tacctttgcg ccaggcgtaa atctgcgctt tgttgatatc gcgagtggtg gcggtatcgg
     1141 ggaaaaacag cgtcagcgac aaaccctgat cgacatggcg agtcgcttcc gcgtaggtgt
     1201 cgatgatctt ttctgcgccg atttcgtaag catcctgata cagcgccaga ttttcgttag
     1261 tcataaacgg ggcagggtag taaacgcgtc ctgttttgcc ctctttgcgt atctctactt
     1321 tcgccacaat cggatgaata ctcgacgtag catggttgat ataagagata gaaccggttg
     1381 gcggcaccgc ctgaagattc tggttgtaaa tgccgtagcg catcacgtcg tcgcgcagtt
     1441 gcgcccacat ctcacgggta ggtaacgtaa taccgctgcg ggcaaacagt tcgccaactt
     1501 tcgccgtttt cggctgccag ttaccttgca gatattggct aaaatattca ccactggcat
     1561 aacgtgactg tttgaacccg gcgaaggttt caccgcgttc gcgcgccagc aacatcgagg
     1621 tacgcagtgc gtgccaggtg atggcataga aatagagatt ggtgaaatcc agtgcttccg
     1681 gcgaaccata agcgatgcct tctcgcgcca gatagccgtg taaattcatc tgccccagtc
     1741 cgatggcgtg tgaggcggca tttccggctt cgatggacgg cacgctgcgg atatgactca
     1801 tatctgacac cgccgttaaa ccgcgcacgg cagtctctac cgtgcgggca aaatcggggg
     1861 aatccatggt gtgcgcaata ttcaacgaac ctaaattgca ggagatatca tggcctgtgc
     1921 gggcatagtc gagattctcg tcatactctg aggcacagtt gacctgcaaa atctctgagc
     1981 agagattact catatttatg cgcccggcga cagggttagc acggtttacc gtgtcttcat
     2041 acatgatgta gggatagccg gactcaaact ggatttctgc cagccgctgg aagaaatcac
     2101 gggcgttgag gtattttttg cggatgcgtt catcggcaac cagttcgtca tagtgttcgc
     2161 tgatggcgat atcggcaaac ggcttgccat aaactcgctc aacgtcatag ggcgaaaaca
     2221 gcgccatctg cgcattctct tttgccagat ggaaagtgat atccgggatc accacgccca
     2281 gcgacagtgt tttaatgcgg attttttcgt cggcattttc ccgttttgta tcgagaaaac
     2341 gcagaatatc gggatgatga gcatgtaaat agactgcacc tgccccctga cgcgcgccga
     2401 gttggttggc ataggaaaat gcgtcttcca gcattttcat caccggaatt acgccagaag
     2461 attgattttc aatacgttta attggcgcgc ccgcttctcg cagattcgac agcaaaaatg
     2521 ccacgccgcc gccgcgtttc gacagttgca gtgcggaatt taccgcccgg ccaatcgact
     2581 ccatattgtc ttcaatacgc agcagaaaac aggaaaccag ttcgccgcgc tgctgcttac
     2641 cgcagttgag gaatgtcggc gtggctggct gaaagcgtcc tgacagcatt tcattggtca
     2701 gttgcaacgc cagcgtcgca tcgccttgtg ccagcgtcag cgccaccatc gtgactcgat
     2761 cggcaaaatc ttccagataa cgtttaccgt cgaatgtctt caacgtatag ctggtgtaaa
     2821 acttccatgc cccgaggaat gtctggaaac gaaaaccgct ggtgtgtgcg tgggcaaaca
     2881 gcgtaatgac aaagtcgcga gagtagcgat taagaacgct ttcatcatag taaccttcgt
     2941 tgaccagcca gttcaggcgc tgctgctggc tactgaaggt cacactgttc ggacgcacat
     3001 gcgtcgccat aaaggcgtca acggcctggc ggtctttatc gaactgaatg cgacctgcgc
     3061 tatcgtagag gttaagcatc gcattcagcg cgtggtaatc catcgtttcc tgcgtcaggc
     3121 attctgcggt tgtcgttgcc aaaattcggt tactccttta cgaacgtttt cgatatcgct
     3181 ttgcgtaccc atgagttcaa aacggtacag ccacggcacg ccgcatttcc gggcaatcac
     3241 atctccggcg cggccatacg cctcaccaaa gttgcgatta ccagaagcaa taacgccgcg
     3301 aagcaacgcc cggttgtgct cgtcgtttaa aaagcgaatt acctatcgtg gcaccgcgcc
     3361 agccgtaccg ccgccgccgt aagagggcac gatcaggatg taaggctcgt ctacctgaat
     3421 ccgttcccgc tcattgagcg ggatgcgcac cgcgggcaga cctaaacgtt cgataaaacg
     3481 ctgcgtgttt tcggagctgc tggagaagta gacgagctgg ctcatgcact ggccgcgtgt
     3541 ggcgctggat gcagacggtt aatcatgtcc ggacggaaac cagaccagct aagatcgcca
     3601 gcaatcacta ccggcaactg acgaaagccc tgagcacgca acgcttccgc tgcttcagga
     3661 acgcgatcga cattaatcat ttcaaaatca aagccccggt tttccatcgc ccgtttggtg
     3721 gcgtggcact gaacgcaatc gttacgagtg taaatagtta tgcgcatgat tcgtatttcc
     3781 gtttaaaatg aagatacggc gcgatgatac gcgtcgggtt gtctctctgt tgatacagag
     3841 atactagatg tagttgaaaa aagattcaac cacacaatat atagcaaata gtgatcgaaa
     3901 ttaccctaga tatgagcgtg acgggggagg gggatttttg tgattcacca ggcaaaaaga
     3961 aaccccgaag acaggcttcg gggttaaaga cgcgtattta ttatcatttt tgcactacga
     4021 tttgcgcatg cttaacagtg cgccgataaa aatacctacc gcagctgctg tacccacgct
     4081 acaccagggt ctttcacgaa caaaagaatc cgcgcagcca acggcatcgc gtgcggcttg
     4141 ctggacgcga gtacgaccat gcattcgcgc cctggtttct ttcagcaatg cctgagcttt
     4201 actgcgtgcg gcttcagctt cccctttggc gtcgctgccc caggacttca atacagattc
     4261 gaggctgtca gctaattgat tgacatcatt ctggatatcc tgcacgccat catcaacgtc
     4321 gttgcggttc ggtcggttaa acatatgatc ccccattaga attcgatgta attttcagtg
     4381 tagaccataa ttttcttaac tgaggttcat cgcggcttta tggacttttc tgaaagcgtt
     4441 gtgaatgcgc aatcaggtaa ggtaggcccg cggttgataa taacgaggat caaatatgta
     4501 tttacgacca gacgaggtgg cgcgcgtact tgaaaaagtc ggttttactg tcgatgtggt
     4561 aacccagaaa gcttatggtt accgccgtgg ggaaaattat gtctatgtta atcgcgaagc
     4621 gcgaatggga cgtaccgcac tggtaattca tccaacatta aaagaacgta gttcgacact
     4681 ggctgaaccc gcttccgata ttaaaacttg cgatcattat caacaatttc cgctctattt
     4741 agcaggcgag cgacacgagc attacggtat cccgcatggc ttcagttcgc gtgttgcgct
     4801 tgaacgttat ttgaatggtt tattt
//
LOCUS       DAZOYL010000275         4819 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZOYL010000275 DAZOYL010000000
VERSION     DAZOYL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.276"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1153
                     /gene="chbC"
                     /locus_tag="ACHUOY_003465"
     CDS             <1..1153
                     /gene="chbC"
                     /locus_tag="ACHUOY_003465"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR0684139.1"
                     /translation="ASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKVD
                     ALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVAEMFTFIVRRNW
                     VIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLASL
                     GSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAAG
                     KTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEPI
                     LFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNTN
                     GSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANALKF"
     gene            1204..1554
                     /gene="chbA"
                     /locus_tag="ACHUOY_003466"
     CDS             1204..1554
                     /gene="chbA"
                     /locus_tag="ACHUOY_003466"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR0684140.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1562..2404
                     /gene="chbR"
                     /locus_tag="ACHUOY_003467"
     CDS             1562..2404
                     /gene="chbR"
                     /locus_tag="ACHUOY_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR0684141.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2509..3861
                     /gene="celF"
                     /locus_tag="ACHUOY_003468"
                     /gene_synonym="chbF"
     CDS             2509..3861
                     /gene="celF"
                     /locus_tag="ACHUOY_003468"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0684142.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            3874..4632
                     /gene="chbG"
                     /locus_tag="ACHUOY_003469"
     CDS             3874..4632
                     /gene="chbG"
                     /locus_tag="ACHUOY_003469"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR0684143.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
BASE COUNT         1204 a         1025 c         1280 g         1310 t
ORIGIN      
        1 cgcctcaacc attgaaacac ttaatggcct gaaaggtatt ggcggcaacg tatataacgg
       61 aacattaggg ataatgtctt taatggcacc gttctttatt ggcatggcgc tggcagaaga
      121 gcgtaaagtc gatgcgctgg cggctgggtt gttatccgtt gcagcattta tgaccgtcac
      181 cccatatagt gtcggtgagg cctatgtggt tggcgcaaac tggttaggtg gagcgaatat
      241 catctccggg attattattg gcctggtggt ggcagaaatg tttaccttta ttgtccgccg
      301 caattgggtc attaaattgc ccgatagcgt acctgcttca gtatcgcgtt ccttctcggc
      361 attaattccc ggctttatta ttctttccgt gatggggatt attgcctggg cgttgaatac
      421 ctggggcacc aacttccatc agatcattat ggataccatc tcaaccccac tggcatcgtt
      481 gggtagcgtg gtgggctggg cctatgtgat ctttgttcca ctgctctggt tcttcggtat
      541 tcatggcgcg ctggcgctga ccgcactgga caacggcatt atgacgccgt gggcgctgga
      601 aaatatcgcg acctatcagc aatatggttc cgtcgaagcg gcgctggcag ccggtaagac
      661 cttccatatc tgggccaagc cgatgctgga ctcctttatt ttccttgggg gcagtggtgc
      721 gactttaggc ctgatcctgg ctatctttat cgcctctcgc cgtgctgatt atcgtcaggt
      781 ggcaaaactg gcgctgccgt ccggcatctt ccagattaac gaaccgattc tgtttggtct
      841 gccaattatc atgaacccgg tgatgtttat cccgtttgta ctggtacaac cgattctggc
      901 ggcaatcacc ctcgcagcgt actacatggg catcattcct ccggtgacca atattgcacc
      961 gtggaccatg ccaaccggtc tgggagcctt ctttaacacc aacggtagcg tcgccgcatt
     1021 gctggttgca ctcttcaacc ttggtatcgc aacgttaatt tatctgccct ttgttgtggt
     1081 ggctaacaaa gcacaaaacg cgattgataa agaagagcgc gaagaagata tcgctaacgc
     1141 cctgaaattt taatcgatgc cggtacgggt aatcgtgccg gtaagaatag agaggaacga
     1201 tgtatgatgg atctcgataa cattcccgat acgcaaacgg aagctgaaga gctggaagaa
     1261 gtggtgatgg ggcttatcat caactccgga caagcgcgca gcctggcgta tgcggcactg
     1321 aaacaggcga agcagggcga ttttgctgca gcaaaagcca tgatggatca gtcacggatg
     1381 gcattgaatg aagcgcatct ggtacagacg aaactgattg aaggcgatgc gggcgaaggt
     1441 aagatgaaag tgagtctggt gctggtccac gctcaggatc atttaatgac gtccatgctt
     1501 gcgcgtgaac tgattactga attaattgag cttcatgaaa aactgaaggc ataaggagtc
     1561 gatgatgcag ccagtgatta acgcgccgga aattgccact gcccgagaac agcagttgtt
     1621 taatggcaaa aacttccatg tgtttatcta taacaaaact gagagtatca gcgggctgca
     1681 tcagcacgac tattatgaat ttactctggt attaaccggg cgttatttcc aggagattaa
     1741 cggtaagcgc gtgttactgg aacggggcga ttttgttttt attccgttag gttcgcacca
     1801 tcaaagtttt tatgagtttg gtgccacgcg tatattgaac gttgggatca gtaaacgctt
     1861 ttttgagcag cattacctgc cgttgttgcc ttattgcttt gtcgcttcgc aggtataccg
     1921 gaccaataac gcttttctca cctatgtgga aacagtgatt tcttcattga atttccgcga
     1981 aacagggctg gaagagtttg ttgagatggt tactttttat gtcattaacc gtttacgtca
     2041 ttaccgcgaa gaacaggtga ttgatgatgt accgcagtgg ctgaaaagta cggtagaaaa
     2101 gatgcatgat aaagagcagt ttagtgaatc ggcgctggag aatatggtga cgttgtcagc
     2161 caaatcacag gaatatttga cgcgagcgac tcaacgatat tatggcaaaa caccaatgca
     2221 gattattaat gagatccgta ttaattttgc caaaaaacaa ctggaaatga ccaactattc
     2281 cgtgacggat attgcgtttg aggccggtta tagtagcccg agtttgttta ttaaaacgtt
     2341 taagaaatta acgtccttta cgcctaagag ctatcgtaag aaattgactg aatttaatca
     2401 gtaagtcgtt atacctgaca attcacatat ctgtcctgtt gctggattat ttatgtccgg
     2461 gggggcagat atgccagata tcagtattct gtactgaagg gagaaattat gagccagaaa
     2521 ttaaaagtcg tcactattgg tggcgggagc agctataccc cggagttact ggaaggattt
     2581 attaagcgtt atcacgaatt gccggtcagc gaattatggc tggtggatgt cgaagatggt
     2641 aaagagaaac tggatatcat ttttgaactc tgccaacgga tgattgataa cgctggcgtc
     2701 ccgatgaagc tttataaaac gctggatcgc cgcgaagcat tgaaagatgc tgatttcgtt
     2761 actacccaac tgcgcgtagg ccaattaccg gcgcgcgaac tggatgaacg tattccatta
     2821 agtcatggtt atcttggcca ggaaaccaac ggcgcgggcg gtctgtttaa aggtctgcgt
     2881 accattccgg tgatttttga catcgtaaaa gatgtcgaag aactttgtcc gaatgcatgg
     2941 gtgattaact tcactaaccc ggcgggaatg gttactgaag ccgtttatcg tcataccgga
     3001 tttaaacgct ttatcggcgt gtgtaatatt ccgatcggca tgaagatgtt tattcacgat
     3061 gttctgatgc tgaaagacag cgatgattta tctatcgatt tgttcggcct caaccatatg
     3121 gtgttcatta aggatgtgct ggtaaatggc aagtcgcgct ttgccgaatt gcttgatggt
     3181 gtggcatccg ggcagttaaa agcatctggc gttaaaaata ttttcgatct gccatttagc
     3241 gaaggcttaa ttcgttctct gaatctgttg ccgtgttctt atttgcttta ttacttcaag
     3301 caaaaagaga tgctggctat tgaaatgggc gaatactaca aaggcggcgc acgagcgcag
     3361 gtagtacaga aagtcgagaa acaacttttt gagctgtata aaaacccgga gttgaaagtt
     3421 aagccgaaag aactggaaca gcgcggtggg gcttattact ctgatgcagc gtgcgaagtg
     3481 atcaacgcta tctacaacga caagcaagct gaacattacg ttaatatccc gcatcatggg
     3541 catattgata atattccggc agactgggcg gtagaaatga cctgtaagct ggggcgcgat
     3601 ggcgcgacgc cacatccgcg cattacgcat ttcgatgata aagtgatggg gctgattcac
     3661 accattaaag gcttcgagat tgctgccagc aacgccgcac ttagcggaga atttaacgat
     3721 gtgttactgg cgctaaacct tagtccgttg gtgcattccg atcgcgatgc tgagctgctg
     3781 gcacgcgaga tgattctggc gcacgagaaa tggctgccaa attttgccga ctgcatcgca
     3841 gagcttaaaa aagcacatta accgaggctg attatggaac gcttactgat tgttaatgcc
     3901 gatgattttg gcttaagcaa aggtcagaac tacggcatta tcgaggcctg tcgcaatggg
     3961 attgtcacat cgacgacggc gctggtgaat gggcaggcta ttgaccatgc ggtgcagttg
     4021 agtcgtgatg aaccgagtct ggccataggg atgtactttg tccttactat gggcaagcca
     4081 ctgacagcta tgccggggtt aacccgcgat ggtgtgctgg gaaaatggat ctggcagttg
     4141 gcagaagaag atgctttacc gctggaagaa attactcagg agcttgccag tcagtatttg
     4201 cgtttcattg agctatttgg acgcaaacct acgcatcttg atagccatca tcatgtgcat
     4261 atgttcccgc agattttccc gattgttgcc aggtttgcgg ctgaagaggg gattgcgttg
     4321 cgcatcgacc gtcagccgct atctaactct ggtgatttac cggctaatct gcgcagttct
     4381 cagggattca gtagcgcgtt ttatggtgaa gagattagcg aggcactgtt cctgcaagtg
     4441 ctggatgacg caagccatcg gggtgatcgt tcgctggagg tgatgtgtca tccggcgttt
     4501 atcgataata ctatccgtca gagtgcttac tgtttcccac gcttaacgga gctggatgtg
     4561 ttgacatcag cgtcgttgaa aggtgctatc gctcagcgtg gttatcggtt ggggagttat
     4621 cgggatgtgt aagtgaggat gtttgccaga tgcggcgtga accttgcatc cggcgattaa
     4681 ttcctacaaa ggcggcgtaa ctgcgccgct tgagactgct gacaaacgca aaactgcctg
     4741 atgcgcttcg cttatcaggc ctacgtgttt cctgcaatat attgaatttg cacagttttg
     4801 taggccggat aaggcgttc
//
LOCUS       DAZOYL010000276         4808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZOYL010000276 DAZOYL010000000
VERSION     DAZOYL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.277"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(44..2113)
                     /gene="glyS"
                     /locus_tag="ACHUOY_003470"
     CDS             complement(44..2113)
                     /gene="glyS"
                     /locus_tag="ACHUOY_003470"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR0684144.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2123..3034)
                     /gene="glyQ"
                     /locus_tag="ACHUOY_003471"
     CDS             complement(2123..3034)
                     /gene="glyQ"
                     /locus_tag="ACHUOY_003471"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR0684145.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3129..3428)
                     /locus_tag="ACHUOY_003472"
     CDS             complement(3129..3428)
                     /locus_tag="ACHUOY_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR0684146.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3603..4598
                     /gene="wecH"
                     /locus_tag="ACHUOY_003473"
     CDS             3603..4598
                     /gene="wecH"
                     /locus_tag="ACHUOY_003473"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR0684147.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4640..>4808)
                     /locus_tag="ACHUOY_003474"
     CDS             complement(4640..>4808)
                     /locus_tag="ACHUOY_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0684148.1"
                     /translation="MVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPK
                     ETQVTQEEYSE"
BASE COUNT         1096 a         1268 c         1221 g         1223 t
ORIGIN      
        1 aaaaaaagcc tgccagatgg caggcttttt aataacggcg ttattactgc aacagcgaaa
       61 tatccgcaac gcgcaggaac agttcgcgca gtttctccag catggtcaga cggttgagac
      121 gcaattcttt gtcatcaacc atgaccatca ctttatcgaa gaaagcatca accggctcac
      181 gcagctctgc cagttcgacc agcgcatcct ggtaacgacc ttccgcaaag tacggctcca
      241 gcttgtcacg tagcaccaca acctgcatcg ccagtttaat ttcttccggc tctttcaggg
      301 tagaggcatt cacgcggtcg ctcagcactt cgtcagattt cgccagaatg ttagatacac
      361 gtttgttcgc cgccgccagt gcagcagctg catccagggt acggaaatgc gataccgctt
      421 tcatacgggc atcgaaatca gccggacgag tcggacgacg cgccagtacc gcctggatgg
      481 tgtcaacggt gtaaccttcg tcctgatacc aggcgcggaa gcgaccgagc ataaagtcga
      541 taacatcatc aactacgttg gcgttagtca gcttatcgcc atacagacgc actgcttctt
      601 cggtcagcgt ttgcagatca aggttgaggt tcttctcaac gataattcgc agcacgccaa
      661 gtgcggcacg acgcagcgca aacgggtctt tgtcgccttt cggatgctga ccgataccga
      721 agatacccgc cagagtatcc atcttatcag caatcgccag cgcacaagct accgggttgg
      781 acggcaggtc atcaccggca aagcgcggct gatactgctc attcagcgcg acggctacgt
      841 cttccgcttc gccatcgtga cgcgcgtagt gcatccccat aacgccttgg gtgtcggtga
      901 actcgaagac catgttggtc atcaggtcac acttagacag caggcccgca cgggttgcgt
      961 ggttaacgtc agcgccaatc tgttcagcaa tccagccagc cagcgcctgg atgcggtcag
     1021 ttttgtcacg cagcgtaccc agctgttgct ggaacagcac ggtttgcagg cgcggcaggt
     1081 tatcttccag acgtttttta cggtcggtat tgaagaagaa ctcggcatcc gccagacgtg
     1141 gacgaacgac tttctcgtta ccagagataa tttgctgcgg atctttcgat tcgatgttgg
     1201 caacgaagat gaagttcggc agcagtttgc catcgttcgc atacaccggg aagtatttct
     1261 ggtcaccttt catggtgtaa accagggctt cagccggcac cgcgaggaat ttctcttcga
     1321 attttgcggt cagaacgacc ggccactcca ccagcgaagc cacttcttcc agcaggcttt
     1381 cgcttaagtc agcgttaccg ccaatcttac gcgctgcttc ttcggcatcg gctttaatct
     1441 tcgctttacg ttcttcgtaa tcggcgatga ctttcccgcg ctcacgcaga atttccggat
     1501 actgatcggc gttgtcgatg gtgaattccg gttcgcccat aaagcggtgg ccgcgaatca
     1561 cgcgatcgga ctgaatgccc agaatggttg ccggaatgac tttgtcgccc agcagcaggg
     1621 tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc gccccaacgc atcagtttcg
     1681 ggatcggcag tttcgccagc gaagtcgcaa ccatattcgg cagtagtgct tcagtgcttt
     1741 cgcccttcac atgggcgcga tacagcagcc attcgccttt atcggtagtc agacgctcag
     1801 cctggtcaac ggtaataccg caaccacgcg cccaaccttc tgccgctttg ctcggtttgc
     1861 cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt ttcgatttca cgatctggtt
     1921 gcgcttccgc caggttagct actttcaacg ccagacgacg cggagcagca aaccattgaa
     1981 cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt aaagttcgca gcaaaggact
     2041 cagccaggct gcgcagtgct tttggtggca gctcttcagt gccgatttcc accagaaaag
     2101 ttttctcaga catagccgcc tcttacttat ctttgttgca catcgggaag ccgagggctt
     2161 cacgggaagc gtagtatgct tctgccactg ctttggtcag ggtgcgaatg cgcagaatgt
     2221 agcgctgacg ctcagtgacg gagatggctt tacgcgcatc cagcaggttg aagctgtggg
     2281 cggctttcag aatacgctcg taggctggca gcggcagcgg attttccagc gccagcagct
     2341 gctgcgcttc tttctcgtac tgctcgaagc aggtgaacag gaaatccaca tccgcgtatt
     2401 cgaagttgta agtggactgc tccacttcgt tctgatggaa cacgtcgccg taggtggttt
     2461 tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct gtctacgccc tgaatgtaca
     2521 tggccagacg ttccagaccg taggtgatct cgccggtaac cggtttacac tccagaccac
     2581 caacctgctg gaagtaagtg aactgcgtca cttccatgcc gtttagccac acttcccagc
     2641 ccagtcccca ggcacccaga gtcgggtttt cccagttatc ttccacgaaa cggatgtcgt
     2701 gaatagtcgg gtccatgccc agctctttca gagaaccgag gtacagctcc tgaatattgt
     2761 ccggcgatgg cttaatgacc acctggaact gatagtagtg ctgtaaacgg ttggggtttt
     2821 cgccgtagcg accatcggtc gggcgacgag aaggctgaac ataagcagcc gccatcggtt
     2881 ctggccccag cgcgcgcaga caggtcattg ggtgagaggt tcccgcgccg acttccatgt
     2941 ccaatggttg aacaatggtg cagccctggc gagcccagta atcctgtaag gtcaggatca
     3001 agccctggaa ggtcctggta tcaaactttt gcatattatt tcgtgctgga tacgtgtgga
     3061 tttaaaggaa gggatcagta tacccgctgg atggaagata tacagtacga aacgggaaaa
     3121 agcagggctt aacgcatgga aagatgcaaa aaatggccat ctgcgtcaaa agaacagaca
     3181 aactgctctt tacggaaggt ataaccgcgc atttcatagc ttccctggaa ctgttcgaag
     3241 gcggtgacgt cgattttttg ctcgccggtg ttataacgtt ttgccgcctg atccttgcat
     3301 aacgcttcca tattcaacga acgcagagga tcaaccttta cccgttgtgc tttctgcacg
     3361 ggtgacggtg tagaccaacc cactagcacc ataagcgcca ttgccggaaa gaaagcgttc
     3421 atcatcatta aattaccgtc tggtttgcag gtgagtctta ttatttatat cggtatagtg
     3481 gctgatatct tacgaatttc cttaagcctc tataagctgt agtgataaaa ctcagattat
     3541 tccagtgcgc caaccaaatg atttatcggt cagacttttt ttaaccagat acagagacac
     3601 agatgcagcc caaaatttac tggattgata acctgcgagg gatagcgtgt ttaatggtgg
     3661 tgatgattca caccactacc tggtatgtga ccaatgctca tagtgttagc cccgtcacat
     3721 gggatatcgc caatgttctg aattctgcct ctcgtgtcag cgtgccgcta tttttcatga
     3781 tttccggcta tctctttttt ggcgaacgca gcgcccagcc gcgccatttc ttgcgtatcg
     3841 gcttatgtct gattttttat agcgcaatcg cactgctcta cattgcgctg tttacctcca
     3901 tcaatatgga gttagcgctg aaaaacctgc tgcaaaagcc agtgttttac cacttgtggt
     3961 ttttcttcgc gattgcggtg atttatctgg tttcaccgct gattcaggtg aagaacgtcg
     4021 gcggaaaaat gttgctggtg ctaatggcgg tgattggcat tatcgctaac ccaaacacag
     4081 tgccgcagaa aattgacggt tttgaatggc tgccaattaa cttatatatc aatggcgata
     4141 ctttttacta cattctgtat ggcatgttgg gccgcgctat agggatgatg gacacacagc
     4201 ataaagcact gtcgtgggtg agcgccgcgc tgtttgcgac gggggttttt attatctctc
     4261 gcgggacatt atatgaattg cagtggcgcg gaaattttgc cgatacctgg tatctttact
     4321 gtgggccgat ggtttttatc tgcgcaatcg cgctattgac tctggttaaa aacacgctgg
     4381 atacgcgtac cattcgcgga cttggcttaa tctcccgcca ttcattgggt atatacggat
     4441 tccacgcctt gattatccat gcgctgcgca cccggggaat tgagcttaaa aattggccaa
     4501 tactggatat tatttggatc ttttgcgcga cgttggcagc gagtttgtta ctttctatgc
     4561 tggtacaacg aatcgacaga aacagattag tgagttaagt aaaagcccgt tcacattgga
     4621 ctgaccgggc ttacgtgaat tattcgctgt attcttcctg ggtaacctgt gtttcttttg
     4681 gcgggtttat tccggcatca cgaatattct tctgcaccgc tactgcacca aacaagctta
     4741 agaagaaagc cagtccataa aaaccttttt cactgagtaa taatgtcgca ttccacagac
     4801 ctaccatc
//
LOCUS       DAZOYL010000277         4800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZOYL010000277 DAZOYL010000000
VERSION     DAZOYL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.278"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(84..2957)
                     /gene="gcvP"
                     /locus_tag="ACHUOY_003475"
     CDS             complement(84..2957)
                     /gene="gcvP"
                     /locus_tag="ACHUOY_003475"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR0684149.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(3076..3465)
                     /gene="gcvH"
                     /locus_tag="ACHUOY_003476"
     CDS             complement(3076..3465)
                     /gene="gcvH"
                     /locus_tag="ACHUOY_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR0684150.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(3489..4583)
                     /gene="gcvT"
                     /locus_tag="ACHUOY_003477"
     CDS             complement(3489..4583)
                     /gene="gcvT"
                     /locus_tag="ACHUOY_003477"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR0684151.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
BASE COUNT         1076 a         1357 c         1249 g         1118 t
ORIGIN      
        1 ggggactgtc tgagcacttt tgtactttgt catctgacta aaaaggcgcc gaagcgcctt
       61 tagaaaatag tcgaatcagt gaattactgg tattcgctaa tcggtacgca ggagcagaac
      121 aggttacggt cgccgtaaac atcatccaga cgtttcaccg tcggccagta tttgtctgcc
      181 acgcctgccg ggaataccgc aacttcacgg ctgtacggat gcgcccactc ggcgaccagt
      241 tcgctctgaa tgtgcggcgc gttcaccagc gggttatctt ccagcggcca gacagcggct
      301 ttcacctggt caatttctgc gcggatagcc agcatcgcgt cgataaagcg atccagttcc
      361 actttgcttt cagattcagt cggttcaacc atcagcgtac ccgccaccgg gaacgacatc
      421 gtcggcgcgt ggaaaccgta gtcgatcagg cgcttggcaa tatccagctc gctgatgccg
      481 gtttcttctt tcagcgggcg aatatcgaga atacattcgt gcgccacgcg accgtcgcga
      541 ccggtataca gcaccgggaa ggcatcctgc aggcggctgg caatatagtt ggcgttgagg
      601 attgccacct ggcttgcttt tttcagccct tctgcgccca tcatgcggat gtacatccag
      661 ctgattggca ggatagaggc gctaccgaac ggtgccgcag aaactgcgcc ctgacgggtt
      721 aacatgcctt cgatttgcac cacgctatga cccggtacaa acggtgccag atgcgctttc
      781 acaccgatcg gtcccatacc cggaccacca ccgccgtgcg gaatgcagaa agttttatgc
      841 aggttgaggt gcgaaacgtc cgcgccaata aagcccggcg aggtgatgcc aacctgggcg
      901 ttcatgttcg cgccatcaag gtaaacctga ccgccgaact gatgcacgac ttcacacact
      961 tcacggatcg tttcttcata cacgccgtgg gtagaaggat aagtcaccat gatacaggag
     1021 aggttatcgc ccgcctgttc cgctttcgcg cgcagatcag tcagatcgat gttgccgttt
     1081 ttatcacacg ccacaaccac cacctgcatt cctgccatat gtgcagaagc ggggttagtt
     1141 ccgtgcgcag aagccgggat caggcagata tcgcgatgcc cttcgttgcg gctttcatga
     1201 taatgacgaa tcgccagcag gcccgcgtat tcgccctgtg cgccggagtt cggctgcata
     1261 caaacggcgt cgtaaccggt cagtttcacc agccagtcag ccagctgcgc aatcatctgc
     1321 tgataacctt cagcctgttc cggcgggcaa aacgggtgca gttcggcaaa ttccggccag
     1381 gtgattggga tcatctcggc ggcggcgttc agtttcatgg tgcaggaacc cagcgggatc
     1441 atcgcctgat tcagcgccag atctttacgc tccagcgagt gcatatagcg catcatttcg
     1501 gtttcgctgt ggtagcgatt aaacaccgga tgggtgagga tttcgtcgtc gcgcagcatc
     1561 gcaggctgga tagagcggct gtcgtgagcc acgtctttgt ccagcgtgtc gatctccagc
     1621 ccgtggttat cgcccagcag cacgctgaaa agctgcatta cgttttcacg cgtggtggtt
     1681 tcatcaaggg tgatccccac ggcgttcagg atatcgctac gcaggttgat ttcagccgct
     1741 tcggcacgcg tcagtacgcc cgctttgtcg gccacttcca cacacaaggt gtcgaaatag
     1801 tgcgcatggc gcagtttcag acctttttgt tgcaggcccg ccgccagaat atcggtcaga
     1861 cggtgaatgc ggttagcgat acgtttcagg ccaaccgggc cgtgataaac ggcatacagg
     1921 ctggcgatgt ttgccagcag tacctgggaa gtacaaatgt tggagttcgc tttctcacga
     1981 cgaatatgtt gctcgcgagt ctgcatcgcc atgcgcagcg cggtattgcc agctgcatct
     2041 ttcgatacac cgataatacg gcccggcatt gagcgtttgt attcatcttt cgccgcaaag
     2101 aatgccgcgt gtgggccacc gtagcccatc ggcacgccga agcgttgcgc cgaaccaaaa
     2161 acaatatccg cgccctgttt acccggcgca gttaacagca ccagcgccat aatatcggcg
     2221 gcaacgctga ccacaatttt gcgtgatttc agttcgctaa taagcgcagt gtagtcgtga
     2281 atttcaccgg tagtgcctac ctgctgtaac agcacgccga agacgtcctg atggtcgagc
     2341 actttttgcg cgtcatcgac aatcacttca aaaccaaagg tttcggcacg agtacggacc
     2401 acatccagcg tttgcggatg cacatcggaa gccacgaaga agcggttggc atttttcagt
     2461 ttgctgacgc gtttcgccat cgccattgct tcggcggcag cggtggcctc gtccagaaga
     2521 gaagcagagg ccatatccag tccagtcaaa tccagcgtta cctgctggaa gttgagcagt
     2581 gcttcaaggc ggccctggga gacttcaggt tgatacggag tgtacgcggt ataccagccc
     2641 ggattttcca gcatgttacg caggataacc ggcggtagct gcacggcggt gtaacccatg
     2701 ccgatgtaag acgtgaagcg tttattgcga ctggcaatag ccttgagttc tgccagtgcg
     2761 gcgtattcgg tcgccggtgc gccaacctgc ggcggggtcg caagctgaat atctttcggt
     2821 acaatctggc cggtcagcgc gtttaacgat tgtgcgccaa cggcattcag catttcttgc
     2881 tgttgcgcgg cgtccggtcc gatatggcgt tcaataaaag cgccgctgtt ttcaagctgg
     2941 cttaacgtct gtgtcatgag cgatggttcc tgaaacgtgc agtgaattgt gaacctctct
     3001 ccttacgaag agagtgaggg tgaggcataa attttcctca ccctgatcct ctcccgcaga
     3061 agaggaataa agccgttact cgtcttctaa caatgcttcg tatgcggtcg catccagcag
     3121 tgattccagt tcgctttcat cgctggcttt gattttaaag atccagccac ctgcatacgg
     3181 ttcgctgttc accagttccg gggaatcgct cagtgcgtcg tttaccgcca cgatttcacc
     3241 gcttactggc gcataaatgt ctgacgccgc ttttactgat tcggcaaccg cgcagtcatc
     3301 gcccgcgcta accgttgcgc ccacttccgg caggtcaaca aacaccatat cgcctaacag
     3361 ctcctgagca tgttcggtaa taccaacggt gtaagtgccg tcggcttctt tacgcagcca
     3421 ttcgtgttct ttgctgtatt tcagttctgc tggtacgttg ctcatcaatc aatctccaaa
     3481 aaagtaaatc acgcgacggc tttgccgtta cgcacaaaaa caggttttgt cactttaacc
     3541 gacatttcac ggttgcgaat ttgcacaatc gccgtttcgc caataccttc cagcacgcgc
     3601 gccagcgcaa tgctgtaacc cagcgtcggg gagaaagtac cgctggtgat aatgccttca
     3661 tgctggttgc cctgcgcatc ggtaaagcgt accggcagtt cattacgcag cacgcctttt
     3721 tcggtcatca ccagaccaac cagtttttct gtaccatgct cacgctgcac ttccagggct
     3781 tcacgaccga taaagtcacg atctgccggt tcccaggcga tagtccagcc catgttggcg
     3841 gctaaaggag aaatagtttc gtccatctcc tgaccataaa gattcatgcc cgcttccaga
     3901 cgcagcgtgt cacgcgcgcc caagccacat ggcttaacac ccgcttccac cagcgcacgc
     3961 cagaaatcgg ccgctttttc attgggcagc gcaatttcat agcccgcttc accggtataa
     4021 ccagtggtgg caataaacag atcgcccgcc tgcacgccaa agaacggttt catcccttcc
     4081 accgcctgac gctgggcgtc attaaacagt gtggcagctt ttgcctgcgc attcggccct
     4141 tgcacggcaa tcatggaaag gtcatcacga acggtaattt cgatgccgaa aggttcagcg
     4201 tgttgggtaa tccaggagag gtctttttcg cgggtggcgg agttaacaac gaggcggaag
     4261 aaatcttcag taaagtagta gacgatgagg tcatctatca caccgccaga ggcattcaac
     4321 atccccgagt aaagggcttt gccgcttttg gtgagcttcg ccacatcgtt cgccagcaga
     4381 taacgcagaa actcccgggt gcggctgccg cggagatcga cgatggtcat atgtgacaca
     4441 tcaaacattc cggcatcggt acgtaccgca tgatgttcgt cgatttgcga accgtaatgc
     4501 agcggcatca tccagccgtg gaaatccacc atgcgagcgc cgcaaagcgt gtgttgttcg
     4561 tacaaaggag tctgttgtgc catcttgtcc tcattgaata agcggggctg acaacttttc
     4621 atggtgaaat tatcaccacg aaacccagca tcggagccac tcccggtccc caacgcaatc
     4681 gttctctttt gcctgaactt accaccgaaa cagactgtta accataaggt aaaattgatc
     4741 atcacattag cttatggtta aaaaacgcca aaatcgcgac agaataaaaa accaaaaaac
//
LOCUS       DAZOYL010000278         4798 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZOYL010000278 DAZOYL010000000
VERSION     DAZOYL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.279"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..429
                     /gene="soxR"
                     /locus_tag="ACHUOY_003478"
     CDS             <1..429
                     /gene="soxR"
                     /locus_tag="ACHUOY_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR0684152.1"
                     /translation="TPGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRY
                     VAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRD
                     ELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            975..2324
                     /gene="ghxP"
                     /locus_tag="ACHUOY_003479"
                     /gene_synonym="yjcD"
     CDS             975..2324
                     /gene="ghxP"
                     /locus_tag="ACHUOY_003479"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR0684153.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2476..4125
                     /locus_tag="ACHUOY_003480"
     CDS             2476..4125
                     /locus_tag="ACHUOY_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR0684154.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4279..>4798)
                     /locus_tag="ACHUOY_003481"
     CDS             complement(4279..>4798)
                     /locus_tag="ACHUOY_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0684155.1"
                     /translation="LYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNI
                     NLIDSKLFCTWLTNTFLEMVICTDSNMANVNFNNANLSNCHFNCSILTKACMFNTRLY
                     RVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVA
                     MKITADIMQHAD"
BASE COUNT         1002 a         1140 c         1329 g         1327 t
ORIGIN      
        1 acccccggcg aagtggcgaa acgcagcggt gtggcggtat cggcgctgca tttctatgaa
       61 agtaaagggt tgattaccag tatccgtaac agcggcaatc agcggcgata taaacgtgat
      121 gtgttgcgat atgttgcaat tatcaaaatt gctcagcgta ttggcattcc gctggcgacc
      181 attggtgaag cgtttggcgt gttgcccgaa gggcatacgt taagtgcgaa agagtggaaa
      241 cagctttcgt cccaatggcg agaagagttg gatcggcgca ttcatacctt agtggcgctg
      301 cgtgacgaac tggacggatg tattggttgt ggctgccttt cgcgcagtga ttgcccgttg
      361 cgtaacccgg gcgaccgctt aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa
      421 caaaactaaa gcgccacaag ggcgctttag tttgttttcc ggtctttgtc tttctctcta
      481 tcccgctggt acacaggagg gtttcccccg acgtcaacac accccattcg agcacgtggt
      541 ggaggttccg gttggtgttg atgctttaat tgtatgtcac cggcatttct tcgccagtgt
      601 aaaagtatac tttttatccg caatattttt gtcatctcag acgatttttt atcgcaatcc
      661 tgaacggtat acggctcgat aacgctgcaa tcttgcgcac cgacgataac gtttgcgcat
      721 caattgcctg gtttttcatc gtcaagacaa taaaagagaa aaaagcagca aacttcggtt
      781 gaaaaagccg ctatgatcgc cggataatcg tttgcttttt ttaccacccg ttttgtatgc
      841 gcggagctaa acgtttgctt ttttgcgacg cagtaaattg tcgcaaacct ggagcaggaa
      901 gataacgttt ctctggcagg ggattgtccg ccacgcatct tgacgaaaat taaactctca
      961 ggggatgttt tcttatgtct acgccatcag cgcgtaccgg cggttcactc gacgcctggt
     1021 ttaaaatttc acaacgtgga agcactgtcc gtcaggaagt ggttgccggg ttaacaacgt
     1081 ttctggcgat ggtctactcg gtcatcgtcg ttccaggtat gttgggtaaa gcaggcttcc
     1141 cgcctgcggc agttttcgtt gcaacctgtc tggttgccgg actcggttct atcgtgatgg
     1201 gtctgtgggc taatctgccg ttggcgattg gttgcgccat ctccctgacg gcgtttaccg
     1261 cattcagcct ggtgctgggg caacatatta gcgtacctgt cgcgctgggt gccgtgttcc
     1321 tgatgggtgt gctgtttacg gtaatttctg ccacgggtat ccgtagctgg attttgcgca
     1381 acttgccgca cggtgtggcg cacggcacgg ggatcggtat cggtctgttc ctgctgctca
     1441 ttgccgctaa tggtgtcggt ctggtgatta aaaacccgct tgatggtctg cccgttgcgc
     1501 tgggtgattt cgcgaccttc ccggtgatta tgtcactggt aggtctggcg gtgatcatcg
     1561 gcctggaaaa actgaaagtc cctggtggca ttctgctgac cattatcggt atctcaattg
     1621 tcggtttgat cttcgatcct aacgtccatt tctccggcgt tttcgccatg ccttcattga
     1681 gcgatgaaaa cggcaattca ctgattggca gcctggacat tatgggcgcg ctgaatcctg
     1741 tagtcctgcc aagcgttctg gcgctggtga tgacggcagt atttgatgcc accggaacta
     1801 tccgtgccgt cgccggccag gcgaacctgc tggataaaga tgggcagatc atcgacggtg
     1861 ggaaagcact gaccactgac tccatgagca gcgttttctc tggcctggtg ggtgctgctc
     1921 cggcagcggt atacatcgag tctgcggcgg gtacggcggc gggcggtaaa accggtttga
     1981 cggctatcac cgttggcgtg ctgttcctgc tgattctgtt cctctctccg ctctcttacc
     2041 tcgttccggg gtatgcaacg gctccggcgc tgatgtacgt tggcctgctg atgctgagca
     2101 acgtggcgaa aatcgacttt gctgattttg ttgatgcgat ggcgggtctg gttacggcgg
     2161 tattcatcgt gctgacctgt aacatcgtaa caggcatcat gatcggcttc gcgactctgg
     2221 tgattggtcg tctggtttcc ggcgaatggc gcaagttgaa catcggtacg gtcgttatcg
     2281 ccgtggcgct ggtgaccttc tatgcgggtg gctgggctat ctaatctttc atcggattct
     2341 gaaaacgggt ggcaatggct gcccgttttt attttctccg cacattgtgt gagcttttgc
     2401 gatattctga aaaaatgaga attcaggcat aacgcctttc tcagatcata aaaaaacatc
     2461 gcaaacaggg aacgcatgga aatcttcttc accatactga taatgaccct cgtggtctca
     2521 ctgtccgggg tggtcactcg tgtcatgccc tttcagatcc cgcttccgct tatgcaaatc
     2581 gccatcggtg cgctactggc gtggccgacg tttggtttgc atgtggagtt tgatcctgaa
     2641 ctctttttag tcttgtttat cccgccgttg ctgttcgctg atggctggaa aacgccgacc
     2701 cgtgaatttc tcgaacatgg tagagagatt ttcggcctcg cgctggcgct ggtggtggtc
     2761 accgtggtcg gcattggctt ccttatttac tgggtggtgc cgggcattcc gctgatcccc
     2821 gcctttgcgc tggcggcggt actttctccg accgatgctg tggcgctctc cgggattgtt
     2881 ggcgaagggc gcatcccgaa aaaaatcatg ggcattttgc agggcgaagc gttgatgaat
     2941 gacgcctccg gcctggtgtc gttgaagttt gccgtggcag tggcgatggg gacgatgatc
     3001 ttcaccgtcg gcggtgcgac ggtcgaattt atgaaagtag ccattggtgg tattcttgcc
     3061 ggttttgtgg tgagctggct gtatggtcgc tcgctgcgat tcctcagccg ctggggcggt
     3121 gatgaacccg cgacgcagat cgttctgctg ttcttgctgc cattcgcttc ttatctgatt
     3181 gccgaacata ttggcgtttc cggcatcctc gctgcggttg ccgccgggat gaccatcacc
     3241 cgctccggtg tgatgcgccg tgcgccgctg gcaatgcgcc tgcgcgcaaa cagcacctgg
     3301 gcgatgctgg aatttgtctt taacggcatg gtgttcctgc tgttaggtct gcagctgccg
     3361 ggtattctgg agacgtcgct gatggcggca gaaatcgacc ctaacgtcga aatctggatg
     3421 ctgtttaccg atattattct gatatatgcg gcgctgatgc tggtccgttt cggctggctg
     3481 tggacgatga aaaagttcag caaccgcttc ctgaagaaga agccgatgga gtttggttcg
     3541 tggaccacac gagaaatcct gatcgcgtct ttcgccgggg tgcgtggggc gatcactctg
     3601 gccggtgtgc tctctatccc gctgctcttg ccggatggta acgtcttccc ggcgcgctat
     3661 gagctggtgt tcctggcggc tggcgtcatt ctcttctcgc tgtttgtcgg cgtggtgatg
     3721 ttgcctattc tgctacaaca cattgaagtc gcggaccatt cgcaacaatt gaaagaagaa
     3781 cgtattgcgc gagcggcaac ggcagaagtg gcgattgtgg cgatccagaa aatggaggag
     3841 cgtctggcgg cggataccga agagaatatc gataaccagc tgcttacgga ggtcagttct
     3901 cgcgtcattg gtaacctgcg tcgtcgcgcc gatggacgta acgacgttga aagttccgtg
     3961 caggaagaga accttgagcg tcgcttccgt ctggcggcat tgcgttctga acgtgctgaa
     4021 ctgtaccacc tgcgcgccac gcgggagatc agcaacgaaa cgctgcaaaa attactgcac
     4081 gatctcgatt tgcttgaagc gttgctaatt gaggaaaatc agtaagccga aagcgacgta
     4141 accaggcccg aggtaatctg aaaaaggtgc aatcttttgc acctttaaca ggttagctcg
     4201 ggctaacatt catgattcta aaacaaataa gttatctctc ttgtaaagtc aattcattgg
     4261 ctgttcttcg acttaacctc aatctgcgtg ttgcataata tctgcagtaa tcttcatagc
     4321 gactgcatta atattatcct cagtttgtga catgccagta tgagaggtac aatcttcttc
     4381 aaagaatttc tgtagtgtta ccagggtatc actatcaatg gggatatttt cctccccacg
     4441 gagtatggaa atgcccattc cctggacgct agcctcatca aaattaaccc gatagagacg
     4501 ggtattaaac atacaggctt ttgttaaaat agaacagttg aaatggcagt tgcttaaatt
     4561 ggcattatta aaattaacat tagccatatt agagtcggta catataacca tttcgaggaa
     4621 tgtatttgtt agccacgtac aaaagagttt gctatcaatt aagtttatat tctggaggat
     4681 tgctttgttc agattgacgc gagacagatc agcatgttca aagttaatta aatcgagttt
     4741 agagaaacta aggtcggctt taaataaatt aacatatggc gctattactt tataaaga
//
LOCUS       DAZOYL010000279         4796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZOYL010000279 DAZOYL010000000
VERSION     DAZOYL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.280"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUOY_003482"
     tRNA            6..81
                     /locus_tag="ACHUOY_003482"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUOY_003483"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUOY_003483"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUOY_003484"
     CDS             complement(299..1057)
                     /locus_tag="ACHUOY_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR0684156.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUOY_003485"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUOY_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR0684157.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHUOY_003486"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHUOY_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="ACHUOY_003487"
     CDS             complement(3427..4452)
                     /locus_tag="ACHUOY_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0684158.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1315 a         1238 c         1146 g         1097 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaggtaa tgactc
//
LOCUS       DAZOYL010000280         4777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZOYL010000280 DAZOYL010000000
VERSION     DAZOYL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.281"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(51..494)
                     /locus_tag="ACHUOY_003488"
     CDS             complement(51..494)
                     /locus_tag="ACHUOY_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0684159.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            651..1580
                     /gene="metA"
                     /locus_tag="ACHUOY_003489"
     CDS             651..1580
                     /gene="metA"
                     /locus_tag="ACHUOY_003489"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR0684160.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1849..3450
                     /gene="aceB"
                     /locus_tag="ACHUOY_003490"
                     /pseudo
     CDS             1849..3450
                     /gene="aceB"
                     /locus_tag="ACHUOY_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3480..4771
                     /gene="aceA"
                     /locus_tag="ACHUOY_003491"
                     /pseudo
     CDS             3480..4771
                     /gene="aceA"
                     /locus_tag="ACHUOY_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1189 a         1259 c         1208 g         1121 t
ORIGIN      
        1 tgcgacgctg gcgcgtcttc tccagcctgg gcaggcacgg caggggcagc ttattcctcc
       61 acatacgcca gattcagcaa cggatacggt ttccccaaat cgtccacctc agagcgtccc
      121 gtaaccttaa aacccacctt cttatagaac ccaaccgcct gctcattttg ctcattaacg
      181 ttggttgtca gttccggtgc catcgagagc gcatgctcca ccagcatccg acccacgccg
      241 cagccgcaca catcaggatc gataaacagc gcatccatat gctgcccact taacaacata
      301 aacccaaccg gctgatcccg ttcattaacc gcgacccaca acggcgcttc cggcaggaag
      361 gaacgaacta gcacctccag ctcggcccga tactctgctg ataaaaaatc gtgtgtggca
      421 tcgacagaac gacaccaaat cgcaacgagt tcctcccctt cctcatgccg tgagcggcga
      481 atactaataa ccattttctc tccttttcgt cattcttata ttctaacgta gccttttcct
      541 tgaaactttc tcaccttcaa catgcaggct cgacattggc aaattttctg gttatcttca
      601 gctatctgga tgtctaaacg tataagcgta tgtagtgagg taatcaggtt atgccgattc
      661 gtgtgccgga cgagctaccc gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga
      721 caacttctcg tgcgtctggt caggaaattc gtccacttaa ggttctgatc cttaacctga
      781 tgccgaagaa gattgaaact gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc
      841 aggtcgatat tcagctgttg cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc
      901 atctgaacaa cttctactgt aactttgaag atattcagga acaaaacttt gacggtctga
      961 tagttaccgg cgcgccgctg ggcctggtgg agtttaatga tgtcgcttac tggccgcaga
     1021 tcaaacaggt gctggagtgg tcgaaagatc acgtcacctc gacgctgttt gtctgctggg
     1081 cggtacaggc cgcgctcaat atcctctacg gcattcctaa gcaaactcgc accgacaaac
     1141 tctctggcgt ttacgagcat catattctcc atcctcatgc gcttctgacg cgtggctttg
     1201 atgattcatt cctggcaccg cattcgcgct atgctgactt tccggcagcg ttgattcgtg
     1261 attacaccga tctggaaatt ctggcagaga cggaagaagg ggatgcatat ctgtttgcca
     1321 gtaaagataa gcgcattgcc tttgtgacgg gccatcccga atatgatgcg caaacgctgg
     1381 cgcaggaata tttccgcgat gtggaagccg gactagaccc ggatgtaccg tataactatt
     1441 tcccgcacaa tgatccgcaa aacaaaccgc gcgcgagctg gcgtagtcac ggtaatttgc
     1501 tgtttaccaa ctggctcaac tattacgtct accagatcac gccatacgat ctacggcaca
     1561 tgaatccaac gctggattaa tcttctgtga tagtcgatcg ttaagcgatt cagcacctta
     1621 cctcaggcac cttcgggtgc cttttttatt tccgaaacgc acctcagcaa gtgaataaat
     1681 tttattcata ttgttatcaa taagttatca agtattttta attaaaatgg aaattgtttt
     1741 tgattttgca ttttaaatga gtagtcttag ttgtgctgaa cgaaaagcgc acaacgatcc
     1801 ttcgttcaca gtggggaagt tttcggatcc atgacgagga gctgcacgat gactgaacag
     1861 gcaacaacaa ccgatgaact ggctttcaca aggccgtatg gcgagcagga gaagcaaatt
     1921 cttactgccg aagcggtaga atttctgact gagctggtga cgcattttac gccacaacgc
     1981 aataaacttc tggcagcgcg cattcagcag cagcaagata ttgataacgg aacgttgcct
     2041 gattttattt cggaaacagc ttccattcgc gatgctgatt ggaaaattcg cgggattcct
     2101 gcggacttag aagaccgccg cgtagagata actggcccgg tagagcgcaa gatggtgatc
     2161 aacgcgctca acgccaatgt gaaagtcttt atggccgatt tcgaagattc actggccccg
     2221 gactggaaca aagtgatcga cgggcaaatt aacctgcgtg atgcagttaa cggcaccatc
     2281 agctatacca atgaagcagg caaaatttat cagctcaagc ccaatccagc ggttttgatt
     2341 tgtcgggtac gcggtctaca cttgccggaa aaacatgtca cctggcgtgg tgaggcaatc
     2401 cccggtagcc tgtttgattt tgcgctctat ttcttccaca actatcaggc actgttggca
     2461 aagggcagcg gtccctattt ctatctgccg aaaacccagt cctggcagga agcggcctgg
     2521 tggagcgaag tcttcagcta tgcagaagat cgctttaatc tgccgcgcgg cgccatcaag
     2581 gcgacgttgc tgattgaaac gctgcccgcc gtgttccaga tggacgaaat ccttcacgcg
     2641 ctgcgcgacc atattgttgg tctgaactgc ggtcgctggg attacatctt cagctatatc
     2701 aaaacgttga aaaactatcc cgatcgtgtc ctgccagaca gacaggccgt gacgatggat
     2761 aaaccattcc tgaatgctta ctcacgcctg ctgattaaaa cctgccataa acgcggtgct
     2821 tttgcgatgg gcggcatggc agcgtttatt ccgagcaaag atgaagagcg taataaccag
     2881 gtgctcaaca aagtaaaagc ggataaagcg ctggaagtca ataacggtca cgatggcaca
     2941 tggatcgctc acccaggcct tgcggacacg gcaatggcgg tattcaacga cattctcggc
     3001 tcccgtaaaa atcagcttga agtattgcgc gaacaagacg cgccgattac tgccgatcag
     3061 ctgctggcac cttgtgacgg tgaacgcacc gaagaaggta tgcgcgccaa cattcgcgtg
     3121 gctgtgcagt acatcgaagc gtggatctcc ggcaacggct gcgtgccgat ttatggcctg
     3181 atggaagatg cggcgacggc tgaaatttcc cgtacctcga tctggcagtg gatccatcat
     3241 caaaaaacgt tgagcaatgg caaaccggtg accaaagcct tgttccgcta gatgctgggc
     3301 gaagagatga aagtcattgc cagcgaactg ggcgaagaac gtttctccca gggccgtttt
     3361 gacgatgccg cacgcttgat ggaacagatc accacttccg atgagttaat tgatttcctg
     3421 accctgccag gctaccgcct gttagcgtaa accaccacat aactatggag catctgcaca
     3481 tgaaaacccg tacacaacaa attgaagaat tacagaaaga gtggactcaa ccgcgttggg
     3541 aaggcattac tcgcccatac agtgcggaag atgtggtgaa attacgcggt tcagtcaatc
     3601 ctgaatgcac gctggcgcaa ctgggcgcag cgaaaatgtg gcgtctgctg cacggtgagt
     3661 cgaaaaaagg ctacatcaac agcctcggtg cactgactgg cggtcaggcg ctgcaacagg
     3721 cgaaagcggg tattgaagca gtctatctgt cgggatggca ggtagcggcg gacgctaacc
     3781 tggcggccag catgtatccg gatcagtcgc tctatccggc aaactcggtg ccagctgtgg
     3841 tggagcggat caacaacacc ttccgtcgtg ccgatcagat ccaatggtcc gcgggcattg
     3901 agccgggcga tccgcgctat gtcgattact tcctgccgat cgttgccgat gcggaagccg
     3961 gttttggcgg tgtcctgaat gcctttgaac tgatgaaagc gatgattgaa gccggtgcag
     4021 cggcagttca cttcgaagat cagctggcgt cagtgaagaa atgcggtcac atgggcggca
     4081 aagttttggt gccaactcaa gaagctattc agaaactggt cgcggcgcgt ctggcagctg
     4141 acgtgacggg cgttccaacc ctactggttg ctcgtaccga tgccgatgca gcggatctga
     4201 tcacctccga ttgcgacccg tatgacagcg aatttattac cggcgagcgt accagtgaag
     4261 gcttcttccg tactcatgcg ggcattgagc aagcgatcag ccgtggcctg gcgtatgcgc
     4321 catatgctga cctggtctgg tgtgaaacct ccacgccgga tctggaactg gcgcgtcgct
     4381 ttgcacaagc tatccacgcg aaatatccgg gcaaactgct ggcttataac tgctcgccgt
     4441 cgttcaactg gcagaaaaac ctcgacgaca aaactattgc cagcttccag cagcagctgt
     4501 cggatatggg ctacaagttc cagttcatca ccctggcagg tatccacagc atgtggttca
     4561 acatgtttga cctggcaaac gcctatgcct agggcgaggg tatgaagcac tacgttgaga
     4621 aagtgcagca gccggaattt gccgctatac cttcgtatct caccagcagg aagtgggtac
     4681 aggttacttc gataaagtga cgactattat tcagggcggc acgtcttcag tcaccgcgct
     4741 gaccggctcc actgaagaat cgcagttcta agcaacc
//
LOCUS       DAZOYL010000281         4735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZOYL010000281 DAZOYL010000000
VERSION     DAZOYL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.282"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            88..1128
                     /gene="pstS"
                     /locus_tag="ACHUOY_003492"
     CDS             88..1128
                     /gene="pstS"
                     /locus_tag="ACHUOY_003492"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR0684161.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            1215..2174
                     /gene="pstC"
                     /locus_tag="ACHUOY_003493"
     CDS             1215..2174
                     /gene="pstC"
                     /locus_tag="ACHUOY_003493"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR0684162.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2174..3064
                     /gene="pstA"
                     /locus_tag="ACHUOY_003494"
     CDS             2174..3064
                     /gene="pstA"
                     /locus_tag="ACHUOY_003494"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR0684163.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3155..3928
                     /gene="pstB"
                     /locus_tag="ACHUOY_003495"
     CDS             3155..3928
                     /gene="pstB"
                     /locus_tag="ACHUOY_003495"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR0684164.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            3943..4668
                     /gene="phoU"
                     /locus_tag="ACHUOY_003496"
     CDS             3943..4668
                     /gene="phoU"
                     /locus_tag="ACHUOY_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR0684165.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1104 a         1189 c         1300 g         1142 t
ORIGIN      
        1 ttccttacat ataactgtca cctgtttgtc ctattttgct tctcgtagcc aacaaacaat
       61 gctttatgaa tcctcccagg agacattatg aaagttatgc gtaccaccgt cgcaactgtt
      121 gtcgccgcga ccttatcgat gagcgctttc tctgtgtttg cagaagcaag cctgacaggt
      181 gcaggtgcaa ccttccctgc gccggtgtat gccaaatggg ctgacactta ccagaaagaa
      241 atcggtaata aagttaacta ccagggtatc ggttcttccg gtggcgtaaa acagattatc
      301 gctaataccg ttgattttgg tgcctctgac tcgccgctgt ctgacgaaaa actggctcag
      361 gaaggtctgt tccagttccc gaccgtgatt ggcggcgtgg tgctggcggt taacattcca
      421 gggctgaagt ctggcgaact ggtgctggat ggtaaaaccc tcggcgacat ctacctgggc
      481 aaaatcaaga agtgggatga tgaaaccatc gccaaactga atccgggtct gaaactgcct
      541 tcacaaaaca ttgctgtagt acgccgcgca gatggctccg ggactacctt cgtcttcacc
      601 agctacctgg cgaaagtgaa cgaagagtgg aaaaacaacg ttggtactgg ctctaccgta
      661 aaatggccga tcggtctggg cggtaaaggt aacgacggta tcgccgcgtt cgttcagcgt
      721 ctgccgggtg caattggtta tgttgaatat gcttacgcga agcagaacaa cctggcgtac
      781 accaaactga tctccgctga tggtaaaccg gttagtccga ccgaagaaaa cttcgctaat
      841 gcagcaaaag gtgcagactg gagcaaaacc ttcgctcagg atctgaccaa ccagaaaggc
      901 gaagatgcat ggcctattac ctctaccacg ttcattctga tccacaaaga tcagaagaaa
      961 ccagaacaag gcacagaagt gctgaaattc ttcgactggg cgtacaaaac cggggctaaa
     1021 caggcgaacg acctggatta cgccagcctg ccggatagtg tagttgaaca ggttcgcgct
     1081 gcgtggaaga ccaatattaa agacagtagc ggtaagccgc tgtactaata aaactccagg
     1141 ccgggtacgg tgttttacgc cgcatccggc attacaaaat gactttgtaa acgcgtttaa
     1201 ctgaagagta acttatggct gcaaccaagc ctgcttttaa cccaccgggt aaaaagggcg
     1261 acataatttt cagcgtgctg gtaaaactgg cggcgctgat tgtgctattg atgttgggtg
     1321 gcattattgt ctctctgatc atctcctcct ggccgagcat tcagaaattt ggtctggctt
     1381 tcctatggac caaagagtgg gatgcaccga acgatatcta cggggcgctg gtgccgatct
     1441 acggtacgtt ggtgacttcg tttatcgcgc tgctgatcgc cgtcccggtg agtttcggta
     1501 tcgccctgtt cctgactgag cttgcgcctg gctggctgaa acgcccgctg ggtatcgcca
     1561 ttgagctgct ggcagccatt ccaagtatcg tttacggcat gtggggcctg tttatctttg
     1621 cgccgctgtt cgccgtttac tttcaggagc cggtcggcaa tatcatgtcg aatatcccga
     1681 ttgttggcgc gctgttctct ggccccgcat ttggtatcgg tatcctcgcg gcaggcgtga
     1741 tcctcgccat catgattatt ccgtacattg cggcggtaat gcgtgatgtg ttcgaacaaa
     1801 ccccggtgat gatgaaagag tcggcctacg gtattggctg caccacctgg gaagttatct
     1861 ggcgtatcgt tcttccgttc accaaaaatg gtgttatcgg cggcatcatg ctggggctgg
     1921 gccgcgcgct cggtgaaacc atggcggtga cctttatcat cggtaacacc taccagctcg
     1981 acagcgcctc gctgtacatg ccgggcaata gtatcacctc tgcgttggcg aacgaatttg
     2041 cggaagcgga atccggtctg cacgttgccg cactgatgga actgggcctg atcctgtttg
     2101 tgattacctt catcgtcctc gccgcatcga agtttatgat tatgcgcctg gctaagaatg
     2161 agggggcacg ctaatggcta tggttgaaat gcaaaccact gcggcgctgg ctgaatctcg
     2221 ccgcaaaatg caggcgcgtc gccgcctcaa aaaccgtatt gcgctgacgc tctcgatggc
     2281 gacgatggcc ttcggcctgt tctggctgat ctggatttta atgtccacca tcactcgcgg
     2341 tatcgacggt atgtcgctgg cgctgttcac tgaaatgacg ccgccgccca atacggaagg
     2401 tggtggtctg gcgaacgccc tggcgggtag cgggctgtta attttgtggg ccacggtatt
     2461 cggtacgccg ctgggcatta tggcggggat ttatctggcg gaatatggtc gtaaatcctg
     2521 gctggcagaa gtgattcgct tcattaacga cattctgctc tccgtgccgt cgattgtggt
     2581 tggtctgttt gtctacacca tcgtggtggc acagatggag cacttctccg gctgggcggg
     2641 cgtaattgcg ctggcgctgt tgcaggtgcc gatcgttatc cgcaccaccg agaacatgct
     2701 gaaactggtg ccggacagcc tgcgtgaagc ggcttatgcg ctgggtacac cgaagtggaa
     2761 gatgatctcc gcgattacgc tgaaagcgtc ggtgtccggg attatgaccg gtatcctgct
     2821 ggcgattgcc cgtattgccg gtgaaaccgc gccgctgctg tttaccgcgc tctccaacca
     2881 gttctggagc acggacatga tgcagccgat cgccaacctg ccggtgacga tctttaagtt
     2941 tgcgatgagc ccgtttgcgg aatggcaaca actggcctgg gccggggtat tgatcattac
     3001 cctgtgcgtc ctgctgctga acattctggc gcgcgttgtt tttgcgaaga ataaacacgg
     3061 ttaacacttt gccggatgcg gcgtgaacgc cttacccggc ctacggtaag cctgattagc
     3121 gaaaagcatc aggtacgatg aggaaaagat tgcaatgagt atggttgaaa ctgccccgag
     3181 taaaattcag gttcgtaatt tgaacttcta ctacggcaaa ttccatgccc tgaaaaacat
     3241 caacctggat atcgctaaaa accaggtaac ggcgtttatc gggccgtccg gctgcggtaa
     3301 atcgacgctg ctgcgtacct tcaacaaaat gtttgaactg tatccggagc agcgcgcaga
     3361 aggtgaaatt ctgcttgatg gcgacaacat cctgaccaac tctcaggata tcgcactgct
     3421 gcgtgcgaaa gtgggcatgg tgttccagaa accgacgccg ttcccgatgt ccatctacga
     3481 caacatcgct tttggtgttc gtctgtttga gaagctctcc cgtgccgaca tggacgagcg
     3541 cgtgcagtgg gcattgacca aagccgcatt gtggaacgaa accaaagata aattgcacca
     3601 gagcggttac tctctctctg gtggtcagca acagcgtctg tgtattgcgc gtggtatcgc
     3661 cattcgcccg gaagtgctgc tgctcgacga accgtgttcg gcgctcgacc ccatctctac
     3721 cgggcgtatt gaagagctga tcaccgaact gaagcaggat tacaccgtgg tgatcgttac
     3781 ccacaacatg cagcaggctg cgcgttgttc cgaccacacg gcgtttatgt acctgggcga
     3841 attgattgag ttcagcaaca cggacgatct gttcaccaag ccagcgaaga aacaaacaga
     3901 agactacatc accggtcgtt acggttaatt caggagtgcg ttatggacag tctcaatctt
     3961 aataaacata tttccggcca gttcaacgcc gaactggaaa gtatccgcac gcaggtgatg
     4021 accatgggtg gcatggtgga gcagcagctt tctgatgcaa tcaccgcgat gcataaccag
     4081 gacagcgatc tggcgaagcg cgtgatcgaa ggcgacaaga acgtcaacat gatggaagtg
     4141 gcgatcgatg aagcctgcgt gcgcattatc gccaaacgtc agccgacggc gagcgacctg
     4201 cgactggtta tggtgatcag taaaaccatt gccgagctgg agcgtattgg cgacgtggcg
     4261 gacaaaatct gccgtactgc gctggagaaa ttctcccagc agcatcagcc gttgctggta
     4321 agtctggagt cgctgggccg tcataccatc cagatgctgc acgacgtgct ggacgcgttc
     4381 gcgcggatgg acattgacga agcggtacgt atttatcgtg aagataaaaa agtcgatcag
     4441 gaatacgaag gtattgttcg tcaactgatg acctacatga tggaagattc gcgtaccatt
     4501 ccgagcgtac ttactgcgct gttctgcgcg cgttctatcg aacgtattgg cgaccgctgc
     4561 cagaatattt gtgagtttat cttctactac gtgaaggggc aggatttccg tcatgtaggc
     4621 ggtgatgagc tggataaact gctggcggga aaagatagcg acaaataatt caccagacaa
     4681 atcccaataa cttaattatt gggatttgtt atatataact ttataaattc ctaaa
//
LOCUS       DAZOYL010000282         4724 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZOYL010000282 DAZOYL010000000
VERSION     DAZOYL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.283"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..737)
                     /locus_tag="ACHUOY_003497"
     CDS             complement(<1..737)
                     /locus_tag="ACHUOY_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR0684166.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUOY_003498"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUOY_003498"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0684167.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUOY_003499"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUOY_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0684168.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="ACHUOY_003500"
     CDS             4397..4678
                     /locus_tag="ACHUOY_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR0684169.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1140 a         1175 c         1182 g         1227 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg atttttattg atatcaacaa taccatttac ataa
//
LOCUS       DAZOYL010000283         4702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZOYL010000283 DAZOYL010000000
VERSION     DAZOYL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.284"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            51..1013
                     /gene="tauA"
                     /locus_tag="ACHUOY_003501"
     CDS             51..1013
                     /gene="tauA"
                     /locus_tag="ACHUOY_003501"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0684170.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1026..1793
                     /gene="tauB"
                     /locus_tag="ACHUOY_003502"
     CDS             1026..1793
                     /gene="tauB"
                     /locus_tag="ACHUOY_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR0684171.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1790..2617
                     /gene="tauC"
                     /locus_tag="ACHUOY_003503"
     CDS             1790..2617
                     /gene="tauC"
                     /locus_tag="ACHUOY_003503"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR0684172.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2614..3465
                     /gene="tauD"
                     /locus_tag="ACHUOY_003504"
     CDS             2614..3465
                     /gene="tauD"
                     /locus_tag="ACHUOY_003504"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR0684173.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3571..4545)
                     /gene="hemB"
                     /locus_tag="ACHUOY_003505"
     CDS             complement(3571..4545)
                     /gene="hemB"
                     /locus_tag="ACHUOY_003505"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR0684174.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1048 a         1216 c         1378 g         1060 t
ORIGIN      
        1 tttttattcc tgaatactgc tcccataaca agacagggga gcagacaatc atggcaattt
       61 catcgcgtaa cacacttctt gccgcactgg cattcatcgc ttttcaggca caggcggtga
      121 acgtcaccgt ggcgtatcaa acctcagccg aaccggcgaa agtggctcag gccgacaaca
      181 cctttgctaa agagagcggg gcgagcgtgg actggcgtaa gtttgacagc ggagccagca
      241 tcgtgcgggc gctggcttct ggcgacgtgc aaatcggcaa cctcggttcc agcccattag
      301 cggttgcagc cagccaacag gtgccgattg aagtcttctt gctggcgtca aaactgggta
      361 actccgaagc gctggtggta aagaaaacta tcagcaaacc cgaagatttg attggcaaac
      421 gcattgccgt accgtttatc tccaccaccc actacagcct gctggcggcg ctgaaacact
      481 gggggattaa accagggcaa gtggagattg tgaacctgca gccgcccgcg attatcgccg
      541 catggcagcg gggtgatatt gatggtgctt atgtctgggc accggcggtt aacgccctgg
      601 aaaaagacgg caaggtgttg accgattctg aacaggtcgg gcagtggggt gcgccaacgc
      661 tggacgtctg ggtggtgcgc aaagattttg ccgagaaaca tcctgaggtc gtgaaagcgt
      721 tcgctaaaag cgccatcgat gctcagcaac cgtacattgc taacccagac gcgtggctga
      781 aacagccgga aaacatcagc aaactggcgc gtttaagcgg cgtgccggaa ggtgacgttc
      841 cggggctggt gaaggggaat acctatctga cgccgcagca acaaacggca gaactgaccg
      901 gaccggtgaa caaagcaatc atcgacaccg cgcagttttt gaaagagcag ggcaaggtcc
      961 cggctgtagc gaatgattac agccagtacg ttacctcgcg cttcgtgcaa taaaaggagg
     1021 cgcagatgct gcaaatctct catctctatg ccgattatgg cggcaaaccg gcactggaag
     1081 atatcaacct gacgctggaa agcggcgagc tactggtggt gctggggccg tccggctgcg
     1141 gtaaaaccac cctgctgaat ctgattgccg gttttgtgcc ttatcagcat ggcagcattc
     1201 aactggcggg taagcgtatt gagggaccgg gagcagagcg cggggtggtt tttcagaatg
     1261 aagggctact accgtggcgc aatgtacagg acaacgtggc gttcggcctg caattggcag
     1321 gtatagagaa aatgcagcga ctggaaatcg cgcaccagat gctgaaaaaa gtggggctgg
     1381 aaggcgcaga aaaacgctat atctggcagc tttccggtgg tcaacgtcag cgggtgggga
     1441 ttgcccgcgc gctggcggcg aatccccagc tgttattact cgacgaaccg tttggtgcgc
     1501 tggacgcctt cacccgcgac cagatgcaaa ctctgctgct gaaactctgg caggagacgg
     1561 gcaagcaggt gctgttgatt acccacgata tagaagaagc ggtgtttatg gcgactgaac
     1621 tggttctgct ttcatccggc cctggccgtg tgctggagcg gctgccgctc aactttgctc
     1681 gccgctttgt tgcgggagag tcgagccgca gcatcaagtc cgatccacaa ttcatcgcca
     1741 tgcgcgaata tgttttaagc cgcgtatttg agcaacggga ggcgttctca tgagtgtgct
     1801 cattaatgaa aaactgcatt cgcggcggct gaaatggcgc tggccgctct cgcgtcaggt
     1861 gaccttaagc attggcacgt tagcggtttt actcactgta tggtggacgg tggcggcgct
     1921 gcaactgatt agcccgctgt ttttgccgcc gccgcaacaa gtactggcaa aactgctcac
     1981 cattgccgga ccgcagggat ttatggacgc cacgctgtgg cagcatctgg cagccagcct
     2041 gacgcgcatt gtgctggcgc tactcgcggc ggtggtgatc ggtattccgg tcgggatcgc
     2101 catggggcta agcccgacgg tgcgcggcat tctggatccg gtaatcgaac tttatcgtcc
     2161 ggtgccgccg ctggcttatt tgccgctgat ggtgatctgg tttggtattg gtgaaacctc
     2221 gaagatctta ttgatctatt tagcgatttt tgcaccggtg gcgatgtcgg tgctggcggg
     2281 ggtgaaaagc gcgcagcagg ttcgcattcg tgccgcccag tcgctgggtg ccagccgtgc
     2341 gcaggtgctg tggtttgtca ttttgcccgg cgcgctgccg gaaatcctca ccggattacg
     2401 cattggtctg ggtgtgggct ggtctacgct ggtagcggcg gagctgattg ccgcgacacg
     2461 cggtttagga tttatggttc agtcagcggg tgaatttctc gcgactgacg tggtactggc
     2521 ggggatcgcg gtgattgcga ttatcgcctt tcttttagaa ctgggtctgc gcgcgttaca
     2581 gcgccgtctg acgccctggc atggagaagt acaatgagtg aacgtctgag tattaccccg
     2641 ctggggccgt atatcggcgc acaaatttcg ggtgccgacc tgacgcgccc gttaagcgat
     2701 aatcagtttg aacagcttta ccatgcggtg ctgcgctatc aggtggtatt tctgcgcgat
     2761 caagctatta cgccgcagca gcaacgcgcg ctggcccagc gttttggcga actgcatatt
     2821 caccctgttt accctcatgc cgaaggggtt gacgagatca tcgtgctgga tacccataac
     2881 gataatccgc cggataacga caactggcat accgatgtga catttattga aacgccaccc
     2941 gcaggggcga ttctggcagc taaagagtta ccttcgaccg gcggtgatac gctctggacc
     3001 agcggtattg cggcctatga ggcgctctct gttcccttcc gccagctgct gagcgggctg
     3061 cgtgcggagc atgatttccg taaatcgttc ccggaataca aataccgcaa aaccgaggag
     3121 gaacatcaac gctggcgcga ggcggtcgcg aaaaatcctc cgttgctaca tccggtggtg
     3181 cgaacgcatc cggtgagcgg taaacaggcg ctgtttgtga atgaaggctt tactacgcga
     3241 attgtcgatg tcagcgagaa agagagcgaa gccttgttag gctttttatt cgcccatatc
     3301 actaaaccgg agtttcaggt gcgttggcgc tggcaaccga atgatattgc gatttgggat
     3361 aaccgcgtga ctcagcacta tgccaatgcc gattacctgc cacagcggcg gattatgcat
     3421 cgggcgacga tccttgggga taaaccgttt tatcgggcgg ggtgatacga gtgcggacgg
     3481 ttccctcgcc cccttgggga gagggttagg gtgagggggc gttcaccgta ctttcaacag
     3541 gttaactccc cctttctgag aggaaaaaaa ttaacgcaga atcttcttct cagccaaatc
     3601 cagcgcaaag tagctgaaaa tcagatccgc acccgcacgc ttaattgaac ctaagctttc
     3661 gagcacgact ttctcttcat ctatagcacc cgccagcgcg gcgaacttaa tcatcgcgta
     3721 ctcaccgctc acctgatacg cgccaatcgg caattcagta cgttcacgca gctcacgcac
     3781 gatgtcgagg tacgctccag caggtttaac catcaggcag tctgcgccct gggcttcatc
     3841 cagcagtgat tcacgaatcg cctcgcgacg gttcattggg ttcatctgat agcttttgcg
     3901 gtcgcctttt aatgcgcttc cggcagcttc acggaacggg ccataaaagg aagaggcgaa
     3961 cttggtcgaa tacgacataa tcgccgtatc tttaaagcct gcagcgtcca gcgcctgacg
     4021 aatcgcctgt acctggccgt ccatcgcggc ggaaggggcg atgaagtctg cgcctgcagc
     4081 agctgcaacc acggcttgct tgcctaaatt ttccagagtc gcgtcgttgt cgacgccatg
     4141 ctcgcacagc acaccgcagt gaccgtgaga agtgtattca cagaaacaag tgtctgacat
     4201 gacgatcatt tctggcacag tctgcttgca gatgcgcgac atacgcgcca ccagtccatc
     4261 ttcccgccag gcgtcgctgc cggtttcatc ggtatggtga gagatgccaa aagtcattac
     4321 ggaacggata ccggcgttgg cgatgcgttc aatttcgcgt gccagatgtt tctctggaat
     4381 gcgcatcacg cctggcatgg cttcaacggc tttgtagtcg tcaatttctt cttcaacaaa
     4441 gatcggcaac accaggtcgt taaggctaag tgttgtctct tcaaacatag cgcgcagcgc
     4501 aggagatttg cgtaggcgac gagggcgttg gattaagtct gtcatggtct gcctgatgtt
     4561 tgtggaatta aggtgcacag tatacctgaa gcggggcagg agtgttttac gaaagttgtc
     4621 gtgatgttga caagaagaga atgaaagaga gaccaggaca tctttgtaat agaaatagca
     4681 ataatttcat attcactgaa aa
//
LOCUS       DAZOYL010000284         4686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZOYL010000284 DAZOYL010000000
VERSION     DAZOYL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.285"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..641
                     /gene="fes"
                     /locus_tag="ACHUOY_003506"
     CDS             <1..641
                     /gene="fes"
                     /locus_tag="ACHUOY_003506"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR0684175.1"
                     /translation="VTAEERPLAVLLDGEFWAQSMPVWPVLTSLTHRQQLPPAVYVLI
                     DAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTVVAGQSFGGLSALYA
                     GLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEGLRIVLEAGIREPMI
                     MRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLWQPLFHDRS"
     gene            644..862
                     /gene="ybdZ"
                     /locus_tag="ACHUOY_003507"
     CDS             644..862
                     /gene="ybdZ"
                     /locus_tag="ACHUOY_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR0684176.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            859..>4686
                     /gene="entF"
                     /locus_tag="ACHUOY_003508"
     CDS             859..>4686
                     /gene="entF"
                     /locus_tag="ACHUOY_003508"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR0684177.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIIS"
BASE COUNT          963 a         1250 c         1393 g         1080 t
ORIGIN      
        1 atgtaacagc agaagaacgt ccgctggcag ttttgctcga tggcgaattt tgggcgcaaa
       61 gtatgcccgt ctggcctgtg ctgacttcgc tgacccatcg tcagcaactt cctcccgccg
      121 tgtatgtgtt gatcgacgct atcgacacca cgcaccgcgc ccacgaactg ccgtgtaatg
      181 cggatttctg gctggcagta cagcaagagt tattacccct ggtgaaagct attgcccctt
      241 ttagcgatcg cgccgatcgc accgtggtcg ccgggcagag ttttggtggg ctgtccgcgc
      301 tgtatgccgg actgcactgg cctgaacgct ttggctgtgt attaagccag tcaggatcgt
      361 actggtggcc gcatcgggac gggcagcaag agggcgtgtt acttgaaaag ctaaaagctg
      421 gtgaagttag cgccgaaggt ctgcgcattg tgctggaagc gggtattcgc gagccgatga
      481 tcatgcgggc caatcaggcg ctgtatgcgc aattgcaccc tataaaagaa tccattttct
      541 ggcgtcaggt tgacggcgga catgatgcgc tttgttggcg cggtggcttg atgcaggggt
      601 taatcgacct ctggcaacca cttttccatg acaggagttg agtatggcat tcagtaatcc
      661 cttcgatgat ccgcagggag cgttttacat tttgcgcaat gcgcaggggc aattcagtct
      721 gtggccgcaa caatgcctct tatcggcagg ctgggacatt gtgtgtcagc cgcagtcaca
      781 ggcgtcctgc cagcagtggc tggaagtcca ctggcgtact ctgacaccgg ccaatttcac
      841 ccagttgcag gaggcacaat gagccagcat ttacctttgg tcgccgcaca gcccggcatc
      901 tggatggcag aaaaactgtc ggatttaccc tccgcctgga gcgtggcgca ttacgttgag
      961 ttaactggag atgttgatgc gccattactg gcccgcgcgg tggttgccgg actagcgcaa
     1021 gcagatacgt tgcggatgcg ttttacggaa gataacggcg aagtctggca gtgggtcgat
     1081 gatgcgctga tattcgaaca gccagaaatt atcgacctgc gaaccaatat tgatccgcac
     1141 ggtactgcgc aggcattaat gctggcggat ttgcaacaag atttgcgcgt cgatagcggt
     1201 aaaccactgg tctttcatca gctgatacat gttgcggata accgctggta ctggtatcag
     1261 cgttatcacc atttgctggt cgatggcttc agtttcccgg ccattactcg ccagatcgcc
     1321 aatatttact gcacatggct gcgtggcgaa ccaacgcctg cttcgccgtt tacgcctttc
     1381 gctgatgtag tggaagagta ccagcaatac cgcgaaagcg aagcctggca gcgtgatgcg
     1441 gcattctggg cagaacagcg tcgtcaactg ccgccgcccg cgtcactttc tccggcacct
     1501 ttaccggggc gcagcgcttc ggcagatatt ctgcgcctga aactggaatt taccgacggg
     1561 gaattccgcc agctggctac gcaactttca ggtgtgcagc gtaccgattt agcccttgcg
     1621 ctggcagcct tgtggctggg gcgattgtgc aatcgcatgg actatgccgc cggatttatc
     1681 tttatgcgtc gactgggctc ggcggcgctg acggctagcg gacccgtgct caacgtttta
     1741 ccgttgggta ttcacattgc ggcgcaagaa acgctgccgg aactggcaac ccgactggcg
     1801 gctcaactga aaaaaatgcg tcgccatcaa cgttacgatg ccgaacaaat tgtccgtgac
     1861 agcgggcgag cggcaggtga tgaaccgctg tttggtccgg tactcaatat taaggtattt
     1921 gattaccaac tggatattcc tggtgttcag gcgcaaaccc ataccctggc aaccggtccg
     1981 gttaatgacc ttgaactggc cctgttcccg gatgaacacg gtgatttgag tattgagatc
     2041 ctcgccaata aacagcgtta cgatgagcca acgttaatcc agcatgctga acgcctgaaa
     2101 atgctgattg cccagttcgc cgcggatccg gcgctgttgt gcggcgatgt cgatattatg
     2161 ctgccaggtg agtatgcgca gctggcgcag atcaacgcca ctcaggttga gattccagaa
     2221 accacgctga gcgcgctggt ggcagaacaa gcggcaaaaa caccggatgc tccggcgctg
     2281 gcagatgcgc gttacctgtt cagctatcgg gaaatgcgcg agcaggtggt ggcgctggcg
     2341 aatctgctgc gtgagcgcgg cgttaaacca ggggacagcg tggcggtggc actaccgcgc
     2401 tcggtctttt tgaccctggc actccatgca atagttgaag ctggcgcggc ctggctaccg
     2461 ctggataccg gctatccgga cgatcgcctg aaaatgatgc tggaagatgc gcgtccgtcg
     2521 ctgttaatta ccaccgacga tcaactgccg cgctttagcg atgttcccaa tttaacaagc
     2581 ctttgctata acgccccgct taccccccag ggcagtgcgc cgctgcaact ttcacaaccg
     2641 caccacacgg cttatatcat ctttacctct ggctccaccg gcaggccgaa aggggtaatg
     2701 gtcgggcaga cggctatcgt taaccgcctg ttatggatgc aaaatcacta cccgcttaca
     2761 ggcgaagatg tcgttgccca aaaaacgccg tgcagttttg atgtctcggt gtgggagttt
     2821 ttctggccgt ttatcgcagg ggcaaaactg gtgatggctg aaccggaagc gcaccgtgac
     2881 ccgctcgcta tgcagcaatt ctttgccgaa tatggcgtaa cgaccacgca ctttgtgccg
     2941 tcgatgctgg cggcatttgt tgcctcgctg actccgcaaa ccgctcgcca gagttgcgcg
     3001 acgttgaaac aggttttctg tagtggtgaa gccttaccgg ctgatttatg ccgtgaatgg
     3061 caacagttaa ccggtgcgcc gttacataat ctgtatggcc cgacggaagc ggcggtagat
     3121 gtcagctggt atccggcttt tggcgaggaa ctggcacagg tgcgcggcag cagtgtgccg
     3181 attggttatc cggtgtggaa catggggctg cgtattcttg atgcgatgat gcatccggtg
     3241 ccgccgggtg tggcgggtga tctctatctt accggcattc aactggcgca gggctatctc
     3301 ggacgccccg atctgaccgc cagccgcttt attgccgatc cttttgctcc tggcgaacgg
     3361 atgtaccgta ccggagacgt tgcccgctgg ctggataacg gcgcggtgga gtacctcggg
     3421 cgcagtgacg atcagctaaa aattcgcggg cagcgtatcg aactgggcga aatcgatcgc
     3481 gtgatgcagg cgctgccgga tgtcgaacaa gccgttaccc acgcctgtgt gattaaccag
     3541 gcggcagcca ctggtggtga tgcgcgtcag ttggtgggct atctggtgtc gcaatcgggc
     3601 ctgccgttgg ataccagcgc attgcaggcg cagcttcgtg aaacattgcc accacatatg
     3661 gtaccggtgg ttctgctgca acttccacag ttacctctta gcgccaacgg caagctggat
     3721 cgcaaagcct taccgttgcc tgaactgaag acacaagcgt cgggacgtgc accgaaagcg
     3781 ggcagtgaaa cgattatcgc cgcggcattc gcgtcgttgc tgggttgtga cgtgcaggat
     3841 gccgatgctg atttctttgc gctgggcggt cattcgctac tggcaatgaa actggcagcg
     3901 cagttaagtc gacagtttgc tcgtcaggtg acgccggggc aggtgatggt cgcgtcaacc
     3961 gtcgccaaac tggcaacgat tattgatggt gaagaggaca gctcccggcg catgggattc
     4021 gaaaccattc tgccgttgcg tgaaggtaat ggcccgacgc tgttttgttt ccatccggcg
     4081 tccggttttg cctggcagtt tagcgtactc tcgcgttatc tcgatccaca atggtcgatt
     4141 attggcattc agtcgccgcg ccctcatgga cccatgcaga cagcgacaaa cctggatgaa
     4201 gtctgcgaag cgcatctggc aacgttactt gagcaacaac cgcacggccc ttattacctg
     4261 ctggggtatt cccttggcgg tacgctggcg cagggtattg cggcgcgact gcgtgcccgt
     4321 ggcgaacagg tggcatttct tggcttgctg gatacctggc cgccagaaac gcaaaactgg
     4381 caggaaaaag aagctaatgg tctggacccg gaagtgctgg cggagattaa ccgcgagcgc
     4441 gaggccttcc tggcggcaca gcagggaagt acttcaacgg agttgtttac caccattgaa
     4501 ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc gtttgacggt
     4561 aaagcgacgt tgtttgttgc tgaacgcacg cttcaggaag gtatgagtcc tgaacgcgcc
     4621 tggtcgccgt ggattgcgga gctggatatc tatcgtcagg attgtgcgca tgtggatatt
     4681 atctct
//
LOCUS       DAZOYL010000285         4679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZOYL010000285 DAZOYL010000000
VERSION     DAZOYL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.286"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1145
                     /gene="pheA"
                     /locus_tag="ACHUOY_003509"
     CDS             <1..1145
                     /gene="pheA"
                     /locus_tag="ACHUOY_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR0684178.1"
                     /translation="LLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDID
                     RERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPHSARIAF
                     LGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENTSSGAI
                     NDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYP
                     HWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQNFTRFVV
                     LARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIHGNPWEE
                     IFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1188..2309)
                     /gene="tyrA"
                     /locus_tag="ACHUOY_003510"
     CDS             complement(1188..2309)
                     /gene="tyrA"
                     /locus_tag="ACHUOY_003510"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR0684179.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(2320..3390)
                     /gene="aroF"
                     /locus_tag="ACHUOY_003511"
     CDS             complement(2320..3390)
                     /gene="aroF"
                     /locus_tag="ACHUOY_003511"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR0684180.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            3599..3964
                     /locus_tag="ACHUOY_003512"
     CDS             3599..3964
                     /locus_tag="ACHUOY_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR0684181.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4114..4632
                     /locus_tag="ACHUOY_003513"
     CDS             4114..4632
                     /locus_tag="ACHUOY_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR0684182.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1243 a         1201 c         1066 g         1169 t
ORIGIN      
        1 cattactggc gctgcgagag aaaatcagcg cgctggatga aaaattatta gcattactgg
       61 cagagcggcg cgaactggcc gtcgaggtgg gaaaagccaa actgctctcg catcgcccgg
      121 tacgagatat tgatcgtgaa cgcgatttac tggaaagatt aattacgctc ggtaaagcgc
      181 accatctgga cgcccattac attactcgcc tgttccagct catcattgaa gattccgtat
      241 taactcagca ggctttgctc cagcaacatc tcaataaaat taatccgcac tcagcacgca
      301 tcgcttttct cggccccaaa ggttcttatt cccatcttgc ggcgcgccag tatgctgccc
      361 gtcactttga gcaattcatt gaaagtggct gcgccaaatt tgccgatatt tttaatcagg
      421 tggaaaccgg ccaggccgac tatgccgtcg taccgattga aaataccagc tccggtgcca
      481 taaacgacgt ttacgatctg ctgcaacata ccagcttgtc gattgttggc gagatgacgt
      541 taactatcga ccattgtttg ttggtctccg gcacgactga tttatccacc attaatacgg
      601 tctacagcca tccgcagcca ttccagcaat gcagcaaatt ccttaatcgt tatcctcact
      661 ggaagattga atataccgaa agtacgtctg cggcaatgga aaaggttgca caggcaaaat
      721 caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac tttgtacggt ttgcaggtac
      781 tggagcgtat tgaagcgaat cagcaacaaa acttcacccg atttgtggtg ttggcacgta
      841 aagccattaa catgtctgac caggttccgg cgaaaacgac gttgttaatg gcgaccgggc
      901 aacaagccgg tgcgctggtt gaagcgttgc tggtgctgcg caaccacaat ctgattatga
      961 cccgtctgga atcacgcccg attcacggta atccatggga agagatattt tatctggata
     1021 ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt gaaagagtta ggggaaatta
     1081 cccgttcaat gaaggtattg ggctgttacc caagtgagaa cgtagtgcct gttgatccaa
     1141 cctgatgaaa aggtgccgga tgatgtgaat catccggcac tggattatta ctggcggttg
     1201 tcattcgcct gacgcaataa cacgcggctt tcactctgaa aacgctgtgc gtaatcgccg
     1261 aaccagtgct ccaccttgcg gaaactgtca ataaacgcct gcttatcgcc ctgctccagc
     1321 aactcaatcg cctcgccgaa acgcttatag taacgtttga ttaacgccag attacgctct
     1381 gacgacataa taatgtcggc ataaagctgc ggatcctgag caaacagtcg cccgaccatc
     1441 gccagctcaa ggcggtaaat cggcgaagag agcgccagaa gttgctcaag ctgaacattt
     1501 tcttctgcca gatgcagccc ataagcaaaa gtagcaaagt ggcgcagagc ctgaataaac
     1561 gccatattct gatcgtgctc gacagcgcta atacgatgca gccgagcgcc ccagacctga
     1621 atttgctcca gaaaccattg gtatgcttcc ggcttacgtc catcacacca gaccacaact
     1681 tgctttgcca ggctaccgct gtccgggccg aacatcgggt gtaaccccag taccgggcca
     1741 tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca ctgatgccag atcaaccaga
     1801 atacaatctt tcggtaaagg cggtaatttg ccgataactt gctcagtaac gtggattggc
     1861 acactaacaa tcaccattcc ggcatcggca acaatatcag ccgctcgatc ccagtcatgt
     1921 tgctccagaa tccgcacctg ataacccgag agtgtcagca tcttctcgaa caggcgtccc
     1981 atctgaccgc caccgccgac gataaccacc ggacgcagtg acggacaaag tgttttaaat
     2041 cctttgtcgt tttcactgga gtaagattca cgcatcaccc gacgcaaaac atcctcaatc
     2101 agatctggcg gtacacccag agcttccgcc tctgcacgac gcgaggccaa catagatgcc
     2161 tcgcgctccg gaacataaat aggcagtcca aagcggcttt tcacctcgcc cacttcagca
     2221 accagttcca gacgcttcgc taataaattc agcagcgctt tatcgacttc atcaatttga
     2281 tcgcgtaatg cggtcaattc agcaaccata ataaacctct taagccacgc gagccgtcag
     2341 ctgcccgttc agatcctgat gaatttcacg cagcaaggca tcggtcattt cccagctaat
     2401 gcaggcatcg gttacggata caccgtattt catttcactg cgcggttgct cagaagactg
     2461 attgccctcg tggatattac tttcgatcat cagaccaata attgagcgat tgccatcttt
     2521 gatttgagca accacggatt ctgccaccgc aggctgacgg cgataatctt tattggaatt
     2581 accgtggctg caatctacca tcagagacgg gcgcagtccc gcctgttcca tctctttttc
     2641 acattgcgca acatccgcag ggctatagtt cggcgcttta ccaccgcgca ggatgacatg
     2701 cccgtccgga ttcccctgag tttgtagcaa cgcaacctgc cctgcctggt taatgccaac
     2761 aaaacggtgc ggctgggcgg cggcgcgcat agcgttaatt gctgttgcca gactgccgtc
     2821 ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag gccatttcac ggtgagtttg
     2881 cgattccgtt gtacgagcac caattgctga ccagctaaac agatcgccca ggtattgcgg
     2941 gctattcgga tctaacgctt ccgtcgccag tggcagtccc atattcacca gctcaagcag
     3001 caatttacgc gcgatctgca gcccggcttc tacatcaaaa gagccatcca tatggggatc
     3061 gttaattaac cctttccagc cgacagtggt acggggtttt tcaaaataga cgcgcattac
     3121 cagatagagg ctatcgctga cctctgcggc aagggcttta aatcgacgag catattccag
     3181 agcagtttcc ggatcatgaa tggaacaagg accacaaact accagcagac gaggatcgcg
     3241 cccggcgata atatctgaaa tggttttacg cgagtcagca atctgggctt cttgttgcag
     3301 gctcaatgga aaagcggcct tcagttgttc cggagtcatt aaaacctgtt cgtcggtaat
     3361 atgtacgtta ttcagcgcgt ctttttgcat gatggcgatc ctgtttatgc tcgtttgcga
     3421 tagttgatcc tcagcgagga tgacgtaacg ataacacata aagtaaagtt ttcaatccat
     3481 atttcgtaca tttttcttta cacgaacaat ttgctcgcgc ttttgatgct tatttcgtac
     3541 aaataaattt acacctcctt taaaaacaat ccgctatgct ttgaaaaagg agaaagaaat
     3601 gatgaaaaag tttatcgccc ccttattggc tttactggtt agcggatgtc agattgatcc
     3661 ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta tggaagatgc
     3721 gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg cggatcgcgg
     3781 tctatacctt aaaggatatg ccgaaggaca aaagaaaact tgccagaccg attttactta
     3841 tgcccgggga ctttccggta aaagctttcc tgccagctgt aacaatgttg aaagtgccag
     3901 ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa tacgattaaa
     3961 ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt tttatttctc
     4021 acattgatga cgatcgcatg aaaaaatgat attttaatga gcattctgaa aaaatatcga
     4081 tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg actgttctta
     4141 cttctgattc tcctgctgac gggagcacca atactcgctc aggagccttc tgacgttgcg
     4201 aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc tgcgctatca
     4261 gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct acaagagaat
     4321 gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga ggcgatgatt
     4381 tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat ggaattaacc
     4441 gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga gtgcattatt
     4501 ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt aaacctggat
     4561 gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc acggaagaaa
     4621 aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag cattacgca
//
LOCUS       DAZOYL010000286         4622 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZOYL010000286 DAZOYL010000000
VERSION     DAZOYL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.287"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(151..3372)
                     /gene="carB"
                     /locus_tag="ACHUOY_003514"
     CDS             complement(151..3372)
                     /gene="carB"
                     /locus_tag="ACHUOY_003514"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR0684183.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3390..4538)
                     /gene="carA"
                     /locus_tag="ACHUOY_003515"
     CDS             complement(3390..4538)
                     /gene="carA"
                     /locus_tag="ACHUOY_003515"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR0684184.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1000 a         1317 c         1198 g         1107 t
ORIGIN      
        1 ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca aaagatttaa
       61 ttaatgggca aaaaaaacgg tcctcatcag agaaccgtat tacttattcg atcaaattag
      121 cggatgaaaa atatccgcca tgacacgcta ttatttgatc tgcgcgtgca tttcctgcac
      181 cgaaattact ttttcggtcg cgtcggcatt cagcgccatc gcggtagcga aaccaccgtt
      241 cagggtggtg tcataatgca ctttatattg cagcgcactg cgacggatca cgcgggagtc
      301 ttcaatcgcg cgacggcctg aagtggtgtt gatgatgtag gtatattcgc cattcttgat
      361 gcggtcctga atgtgcggac ggccttcatg caccttgttc accagacgcg gattaatgcc
      421 cgcttcgccc agcacaatcg ccgtgccgtg ggtcgcatcc agctcgaagc cctgtttcag
      481 cagttttgcc gccaggtcca ccacgcgttc tttatcgcct tcgcgcacgg aaagcaacgc
      541 acgaccgtgt ttcttcatgg tggagttgct gcccagctgc gctttggcaa acgcttcagc
      601 gaaggtgcgg cccacgccca tcacttcccc ggtggagcgc atttctggcc ctaacagcgg
      661 gtcaacaccc gggaatttat tgaacggcag caccacttct ttcaccgagt agtacggcgg
      721 gataacttct ttggttacgc cctgctcagc cagcgatttg ccagccatca cacgcgccgc
      781 cacttttgcc agcggtacgc cggtggcttt ggagacgaac ggaacggtac gcgccgcacg
      841 cgggttaact tcaatcaggt agacttcgtt gtttttcacc gcaaactgca cgttcatcag
      901 accgcgcacc tgcaattcga aggccagttt ctgcacctgc tggcgcatca catcctgaat
      961 ttcctgactt aaggtgtagg ctggcagaga acatgcggag tcaccggagt gcacgcccgc
     1021 ctgctcgatg tgctccatga tgccgccaat cagcaccatt tcgccgtcgc agatggcatc
     1081 cacgtcaact tctaccgcgt catcaaggaa gtgatccagc aacactggcg catcgttaga
     1141 cacgctgacc gccgtctgga agtagcgacg caggtcagct tcgtcataga cgatttccat
     1201 cgcccgaccg ccgagaacgt aagacggacg taccaccagt gggtagccaa tctctttcgc
     1261 cttctctacc gccatttcaa tagcggtaac ggtggcgttc gccggttgtt tcagtttcag
     1321 acgctcaacc gcatgctgga agcgttcacg gtcttctgca cggtcgatag catccgggct
     1381 ggtgccgata accggtacgc cagcagcttc cagcgcgcgc gccagtttca gcggggtctg
     1441 accgccgtac tgaacgataa cgcctttcgg cttctcaata cgcacgattt ccagcacatc
     1501 ttccagagtt accggctcga agtagaggcg atcggaagtg tcgtagtcgg tggagacggt
     1561 ttccgggtta cagttaacca taatggtttc gtaaccgtct tcgcgcagcg ccagcgaggc
     1621 gtgtacacag cagtagtcga attcgatacc ctgaccgata cggttcgggc cgccgccgag
     1681 aaccatgatt ttttcacggt cggtagacgg attcgcttcg cactcttctt cataagtgga
     1741 gtacatgtaa gcggtgtcgg tggcgaactc tgccgcacag gtatccacgc gcttataaac
     1801 cgggtgcagg tcatactggt cgcgcagctt gcggatttcc gcttcgcgca cgcccgccag
     1861 ttttgccaga cgcgcatcgg caaagccttt gcgtttcagc tggcgcagga attcagcgtt
     1921 caggccagtg atgcccactt ccgctacttt ctcttccaga cgcaccagtt cttcaatctg
     1981 taccaggaac cagcggtcaa tattggtcag gttgaacacg ccgtccacag acaggcccgc
     2041 acggaaggca tcggcgatgt accagatacg ctctgcgcca gcatctttca gttcgcgacg
     2101 gattttggtt aacgcttccg ggtcatcgag gctcactttc gggtcgaatc cagtcgcacc
     2161 gacttccagg ccgcgcagcg ctttttgcag ggattcctgc tgcgtgcgac caatcgccat
     2221 cacttcgcca accgatttca tctgagtggt cagacggtcg ttagcaccag cgaatttttc
     2281 gaagttgaag cgaggaattt tagtaaccac atagtcgatg gacggctcga aggaggccgg
     2341 agtacgtccg ccagtgatgt cgttcatcag ttcgtcgagg gtgtaaccca ccgccagttt
     2401 cgccgccact ttagcaatcg ggaaaccggt cgctttcgac gccagcgccg aagaacggga
     2461 cacgcgtggg ttcatttcga taacaatcag gcgaccgttt ttcgggttca ccgcaaactg
     2521 gacattggaa ccaccggttt caacgccgat ttcacgcagc accgccatcg aggcgttacg
     2581 catgatttga tattctttgt cggtcagcgt ttgggctggc gcgacagtga tggagtcacc
     2641 ggtatggatg cccatcgcat caaagttttc gatagagcag acgatgatgc agttgtcgtt
     2701 tttatcacgc accacttcca tctcgtactc tttccagccg atcagcgact catcaatcag
     2761 cagctctttg gttggggaga gatccagacc gcgggcgcaa atttcttcaa actcttcgcg
     2821 gttataagcg ataccgccgc cgctaccgcc catggtaaag gatgggcgaa taatgcacgg
     2881 gaagcccacg tcagcggcaa ccgccagcgc ttcttccatc gtgtgtgcga taccggaacg
     2941 cgcggtttcc agaccaattt tcttcatcgc tacgtcgaaa cgacggcggt cttctgcttt
     3001 atcaatcgca tcggcggtcg caccaatcat agtcacgccg aactcttcca acacgccctg
     3061 acgctccagc tccagcgcgc agttcagcgc cgtctgaccg cccatcgttg gcagcaccgc
     3121 gtccgggcgc tctttttcaa taatcttgcg tactacttcc cagtgaatcg gctcgatgta
     3181 ggtcgcatcg gccatttccg ggtcggtcat gatggtcgcc gggttggagt tcaccagaat
     3241 gacgcggtaa ccctcttcgc gcagggcttt acatgcttgc gcgccagagt agtcaaactc
     3301 acacgcctga ccgataacaa tcgggcccgc acccagaatc aggatacttt ttatatctgt
     3361 acgttttggc atggctcttt tactcctgat tacttagcgg ttttacggta ctgctcaatt
     3421 aactcgataa agtggtcgaa caacggcgct gcgtcgtgtg gcccagggct ggcttccggg
     3481 tgaccctgga agctgaacgc cggtttatca gtgcgatgaa tgccctgtaa cgtaccgtcg
     3541 aacagggatt tatgcgtgac acgcaggttt gcaggtaatg ttgcttcatc caccgcaaaa
     3601 ccgtggttct gggcggtgat catcaccacg tttttctcca catctttaac cggatggttg
     3661 ccgccgtggt gaccaaattt cattttgaca gtcttcgcac cgctcgccag cgccagcagc
     3721 tgatgaccga ggcagatgcc gaataccgga atatcggttt cgaggaattt ctggatggcg
     3781 gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt tggagaggaa gatgccgtct
     3841 ggattcattt tcagcacatc ttccgcagaa gtttgcgccg gaacgatggt caggcgacag
     3901 cctctatcca ccagcatccg caggatgttg cgcttggcac caaaatcata agccacgacg
     3961 tggaacggca gctcgtcttc ttttttcgct tctggcaggc cgccggtcaa cgtccagctc
     4021 ccttgtgtcc agctataggc ttctgcggtg gtcacttctt ttgccagatc catgccattc
     4081 agacctggga acgcgcgggc tttttctaac gccaacgccg catccgggtt atcgcctgcg
     4141 ataatgcagc cattctgtgc gcctttctcg cgcagtaaac gcgtcagctt acgggtatcg
     4201 atatcggcaa tcgccacgat gttatggcgc ttcaggtaag aagagaggtc ttcggtatta
     4261 cggaagttgc tggcaatcag cggcagatcg cgaatcacca gaccttgtgc atgtacctga
     4321 gaagattctt catcggcgtc attagtgccg acattgccaa tatggggata agtaagagta
     4381 acgatttgac gagaatagga aggatcagtg aggatttctt gataaccggt cattgaagta
     4441 ttgaaaacga cttccccaac cgccgaacct gttgccccta tggcccgacc gtgaaactgg
     4501 gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa caccctccag agaatattca
     4561 ctcactttat ttgcatatta attcacaatg atgacgtaaa tcaatgcaaa tctgcttacc
     4621 ga
//
LOCUS       DAZOYL010000287         4607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZOYL010000287 DAZOYL010000000
VERSION     DAZOYL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.288"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..2162
                     /locus_tag="ACHUOY_003516"
     CDS             <1..2162
                     /locus_tag="ACHUOY_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR0684185.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2396..3679)
                     /locus_tag="ACHUOY_003517"
     CDS             complement(2396..3679)
                     /locus_tag="ACHUOY_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR0684186.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3814..>4607)
                     /locus_tag="ACHUOY_003518"
     CDS             complement(3814..>4607)
                     /locus_tag="ACHUOY_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0684187.1"
                     /translation="QKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKA
                     ASAKLIRSTLSDAFREAIAEGHITTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESS
                     PCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGI
                     SMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELR
                     SLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1093 a         1142 c         1194 g         1178 t
ORIGIN      
        1 aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac
       61 ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga
      121 ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata
      181 actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg
      241 cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata
      301 tgcgtgagat gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt
      361 acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc
      421 ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac
      481 ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa
      541 acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta
      601 ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct
      661 ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact
      721 gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg
      781 ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac
      841 tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc
      901 acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc
      961 aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg
     1021 cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac
     1081 agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg
     1141 caatcatcag aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca
     1201 acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag
     1261 ctaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg
     1321 caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt
     1381 acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa
     1441 ctcagcaacc gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcgctga
     1501 ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg
     1561 aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt
     1621 ttaccctgtc tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg
     1681 aaaatcagcg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     1741 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     1801 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     1861 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     1921 actgggggat gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     1981 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     2041 tgatccatga agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg
     2101 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     2161 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg
     2221 catcttatcc gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc
     2281 atccggcact tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc
     2341 gaaccgtagg ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact
     2401 tcttcgcctc tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt
     2461 tgtagttagt gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat
     2521 tacccgcaaa cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac
     2581 cttcgttgat ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca
     2641 gcgagcggcc cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga
     2701 ggaaaccgta gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag
     2761 tacgtgagtt caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa
     2821 cgatatccac atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg
     2881 tttccagacg gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa
     2941 tgttaacgtt gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt
     3001 tacctgcatc tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc
     3061 cattgttttg tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc
     3121 tctggcagct atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt
     3181 tgacgtcgtg acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca
     3241 catcaatcgg tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat
     3301 ataccgttcc ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct
     3361 tgataattgc cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg
     3421 ccgggccgac aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg
     3481 cagcacctgg tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg
     3541 ccgacagaat cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg
     3601 gcggggttga gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg
     3661 aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga
     3721 agcctgcttt tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca
     3781 acgaacaggt cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc
     3841 cctgcctctg tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg
     3901 ttgagcaaac ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc
     3961 gtgaaaggta ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa
     4021 ataccttgat actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc
     4081 tccgccaaga atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc
     4141 aacatgcaat gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag
     4201 atatccatct acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt
     4261 aacaacagcc agttccattg caagtctgag ccaacatggt gatgattctg ctgtttgata
     4321 aattttcagg tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg
     4381 agtggcagcg accggttttg ttgttatatg gccttcagct attgcctctc ggaatgcatc
     4441 gctcagtgtt gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt
     4501 gagcattgcc gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct
     4561 ccttattgct ttaattttgc tcatgtaatt tatgagtgtc ttctgct
//
LOCUS       DAZOYL010000288         4587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZOYL010000288 DAZOYL010000000
VERSION     DAZOYL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.289"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..762)
                     /gene="idnO"
                     /locus_tag="ACHUOY_003519"
     CDS             complement(<1..762)
                     /gene="idnO"
                     /locus_tag="ACHUOY_003519"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR0684188.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(786..1817)
                     /gene="idnD"
                     /locus_tag="ACHUOY_003520"
     CDS             complement(786..1817)
                     /gene="idnD"
                     /locus_tag="ACHUOY_003520"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR0684189.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            2034..2597
                     /gene="idnK"
                     /locus_tag="ACHUOY_003521"
     CDS             2034..2597
                     /gene="idnK"
                     /locus_tag="ACHUOY_003521"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0684190.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            complement(2601..3620)
                     /gene="ahr"
                     /locus_tag="ACHUOY_003522"
     CDS             complement(2601..3620)
                     /gene="ahr"
                     /locus_tag="ACHUOY_003522"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR0684191.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            3816..3900
                     /locus_tag="ACHUOY_003523"
     tRNA            3816..3900
                     /locus_tag="ACHUOY_003523"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3850..3852,aa:Leu,seq:caa)
     gene            4086..>4587
                     /locus_tag="ACHUOY_003524"
     CDS             4086..>4587
                     /locus_tag="ACHUOY_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0684192.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
BASE COUNT         1199 a         1127 c         1082 g         1179 t
ORIGIN      
        1 aacagccact aacatgccgc catcaacaaa caacaggtgg ccgtttacga aatcagaggc
       61 ttttgaagaa aggaacaccg cagcaccaat cagttcctgc ggatctcccc agcgtgcggc
      121 gggggtccgt ttgcacaacc aggcggtgaa ggcttcgtcc tcaaccagtg ctttagtcat
      181 ttctgttttg aaatagcccg gcgcaatacc gttgacctga atattgtggc gcgccagctc
      241 gacgcacatg ccgcgggtga gcatttttac cgcccctttc gatgcggcat aaggggtgat
      301 ggtgtcacgt cccagttcgc tttgcatcga gcaaatatta ataactttac ctgccttgcg
      361 ttcaaccatg tgacgagtta ccgcttgcga taccaggaac acggcggtct ggtttactgc
      421 gatcacatca ttccactctt gttcagggaa ctcagtaaaa ggatgacggc gctggatacc
      481 ggcgttattc accagcacat caatggggcc gatgtccttt tcgatatgtt caacggcggc
      541 atcaatttca tgtttatgag taacattaaa aggtgcggca acggcctgaa taccctcctg
      601 gtggagtttt tctacagcaa gttctgcgcg ttcggcagta atatcattaa taattatttg
      661 tgcgccatat ttacccaggc cggttgccag taaatagcca atgccctgtg ctgaaccggt
      721 aatcaagata ttttttcctg ccagtgaaaa tagatcgttc attgtattat tcctttattt
      781 atctcttaga aaacaagctg gacttttgct gcctgggttt tatcaccggc gaaacgtagc
      841 gcctcttcca ggtcagtgaa gagatattca gcactcagta aaggcagtgg attgataacg
      901 ccattcgcca gccatgacac tgcggtatta aattcgctgg taaaacggaa agagcctttg
      961 agtgaaatct ccttaccaat caacgtcatc attgggaatt ctgccatcgc gcctcccatc
     1021 cctacctgca ccattacgcc gcgtgcacga gtgacctcca gacaggtatt cactgatgaa
     1081 ggatgaccgg acacttcaaa gctgacatcg aaatggcctt tttccgcttt ccagtgatcc
     1141 atgtcgtcgt tttgtgggtt tacgagcaca tccgcaccca tctctttggc cagcgaaagg
     1201 gaacggggac tcacatcggc acagacaatt tccgcggacc ccagtgtttt cacagcactg
     1261 acaatcaggc agccaatggg cccaacaccg gaaacaaata ctcgcttgcc ctgtaactcg
     1321 tcggcctgat gtgcggcatg aatcgcgacg gctaaaggtt cggcaaaagc cataaccttt
     1381 tcgtcagctt tggccggata agggacacat tgcgacgttt cgaccatttt ataacgggta
     1441 aaaccaccat caacatgagg gaaatacatg gcactgccaa aaaaacgcat atctgtacac
     1501 tgattctcgt tatgttcaat gcagtatttg cagtgaccgc acggtttaga cggattaatg
     1561 gctaccgttt gcccttcatg taattctgat gagtcgctat gaataacttt accgataact
     1621 tcatgaccta acaccatcgg tgcctttatc atgaaattac ctacttttcc ttcctgataa
     1681 taatgtaaat cggaaccgca aattccacct cgggttattt gtactaatgt tccattatta
     1741 ttccaatcta tcgtctgctc ggtaacggca acagttttct tgtccgcaac aacgcaggac
     1801 tgtgttttca cttgcataaa ttcctcactg gtcaggtaga cacctcggaa gcatttaagc
     1861 ggttttaatt gtcattattt gtgatgaaga tcacgtcaga aaattgttac attactatgt
     1921 tacgcataac gtgatgtgcc ttgtaattct tatcagcaga aataaaaaaa cgtgaaatta
     1981 ttatgccacc aggcgtagta tcgcagcagg taagatgatt caggagattt taaatggcgg
     2041 gtgaaagctt tattttgatg ggcgtttcag ggagtggtaa aacattaatt ggtagcaagg
     2101 ttgccgcgtt attatctgct aaatttattg atggtgacga tcttcatcca gccaaaaata
     2161 tagataaaat gtcgcagggt attccattat ctgatgaaga tcgacttccc tggctggaac
     2221 gcttaaatga tgcttcatac agtctttata aaaagaatga aacaggattt attgtctgtt
     2281 catcattaaa aaaacagtat cgtgatattt tacgcaaggg tagcccccat gttcatttcc
     2341 tctggttaga tggcgactat gaaactattc tcgcgcgaat gcagcggcgg gctgggcatt
     2401 ttatgccggt agcgttacta aaaagtcagt ttgaggcgct ggagcgtcca caagcagatg
     2461 aacaggatat tgtacgcatt gatatcaacc atgatattgc caatgtcacc gaacaatgtc
     2521 ggcaggccgt gctggcgata cgacaagaca gaatatgtgc gaaagggggt agcgcctcag
     2581 atcagcgctg cgaatgattt tcaaaaatcg gctttcagaa ccacgcggta acgcgcctta
     2641 ccgttgcgca catgctggat ggcgtcgttt attttcgaca tcgggaacag ttcggtggtc
     2701 ggcgcaacct tgctgcgggc cgcaaagcgc atcagcttac gaagctcata aggtgtgccg
     2761 gttgctgaac cagagacgct gcggtcgccc gcaattaacg taaaggccgg aacagacagc
     2821 ggcgtgagga ccgcaccgac ggtgtggaaa ttaccgccat acgtcagcgc ctcaaaataa
     2881 ggctgccagt cgaggctgac gttgacggtg ttgataatga gatcgaactg ccccgcaagt
     2941 gttttcagtg cctgcggatc gcggctattc accactttat cggcacccat cgccagtact
     3001 tcctgctctt tcgcaggatt cgaactaaag gctgtcacct cgcatcccat tgcgtgcaga
     3061 agttttatag cgatatgccc cagcccgcca ataccaatta ccccaacgcg gctggtagca
     3121 gtgatatggt gcatcaacag tggtttaaag accgtgatac caccacacag cagcggcccg
     3181 gcggactcga tatcgatatt ttccggcagt gggatcaccc attgccagtc tgcacgcaac
     3241 ttctcggcaa aaccgccgcg attcataatt gttggcaccg caccttgctc gcagttgatc
     3301 tgattaccgc taatacaggc gtcgcaatgc ccgcagctac gcgccgtcca gccaatcccg
     3361 acacgctgac cgacctgcaa acctttatcc tgcgcggcgc tcccgagcgc caccacgcgc
     3421 ccaatcacct catgcccggc aaccagcgga tactgtgaaa atccccattc gttgtcgatc
     3481 atcgacagat cggaatggca gatcccgcag taatccacct gcacttcaac atcttgtggc
     3541 ttcaattcac cgggctcgta ctcataaact tccagttcgc cgcccgcttc ttttgcggca
     3601 tagcttttta tcatcgacat tttttggtcc ttctctggtg ttgtctggga agtgtagagc
     3661 atggcagggc ggggtgcctg gagtgtgaca aaggttacac atcgctgtat gcaatgctga
     3721 aaatttcagc acttagcgag gtgcgagcaa gctggcgctt gcatggtggc gtgcgacagg
     3781 tataatccac aacgttttcc gcatacctct tcagtgccga agtggcgaaa tcggtagacg
     3841 cagttgattc aaaatcaacc gtagaaatac gtgccggttc gagtccggcc ttcggcacca
     3901 aaagtatgtc aatagacctc aactgaggtc ttttttatgc ctgaaattca gtatttatct
     3961 acctttcccg ctatattaac tctctcaagg tcaaccgata tcaacgtact tctaccaaca
     4021 tatgttggta cagatgatgg tatttccggt tcgataatgc ttgtaccaac agggagggaa
     4081 tacgcatggc attaacagac atcaaagtca gaacagctaa gccaacagat aagcaatata
     4141 agttgactga tggtggcggt atgcatctgc ttgtccatcc aaatggttct aagtactggc
     4201 gtttgcagta ccgttatgag ggaaagcaaa agatgctggc acttggggtt tatcctgaaa
     4261 tcacactcgc ggatgccaga atacgtcgtg acgagccacg taagctgctt gcgaatggcg
     4321 tcgatccggg agacaaaaag aaaagtgata aggttgaaca gagtaaagca cgaaccttta
     4381 aagaagtcgc gattgagtgg catggcacca ataaaaagtg gtctgaagat catgcccatc
     4441 gtgtgctaaa aagtctggaa gataatcttt ttgcagcgct tggtgaacgt aatatcgctg
     4501 agttaaaaac tcgagattta ttagcaccta ttaaggccgt agaaatgtct ggacgtcttg
     4561 aaatggctgc tcgtcttcaa cagcgca
//
LOCUS       DAZOYL010000289         4523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZOYL010000289 DAZOYL010000000
VERSION     DAZOYL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.290"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            5..1138
                     /locus_tag="ACHUOY_003525"
     CDS             5..1138
                     /locus_tag="ACHUOY_003525"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0684193.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1227..1760
                     /gene="blc"
                     /locus_tag="ACHUOY_003526"
     CDS             1227..1760
                     /gene="blc"
                     /locus_tag="ACHUOY_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0684194.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1757..2074)
                     /gene="sugE"
                     /locus_tag="ACHUOY_003527"
     CDS             complement(1757..2074)
                     /gene="sugE"
                     /locus_tag="ACHUOY_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0684195.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2249..2395)
                     /gene="ecnB"
                     /locus_tag="ACHUOY_003528"
     CDS             complement(2249..2395)
                     /gene="ecnB"
                     /locus_tag="ACHUOY_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0684196.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2506..2631)
                     /gene="ecnA"
                     /locus_tag="ACHUOY_003529"
     CDS             complement(2506..2631)
                     /gene="ecnA"
                     /locus_tag="ACHUOY_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0684197.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2683..3249)
                     /gene="efp"
                     /locus_tag="ACHUOY_003530"
     CDS             complement(2683..3249)
                     /gene="efp"
                     /locus_tag="ACHUOY_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0684198.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3291..4319
                     /gene="epmB"
                     /locus_tag="ACHUOY_003531"
     CDS             3291..4319
                     /gene="epmB"
                     /locus_tag="ACHUOY_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0684199.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1162 a         1151 c         1132 g         1078 t
ORIGIN      
        1 ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc
       61 tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca
      121 aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg
      181 gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg
      241 ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat
      301 caagttaagc gatcccgcaa caaaatactg gcctgaactt actgctaaac agtggaatgg
      361 gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc aggtgccgga
      421 tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc
      481 tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt
      541 gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa
      601 actcaaccat acgtggatta atgtacctcc cccagaagaa aagaattacg cctggggata
      661 tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt
      721 gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttaaccc cccttgatat
      781 caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg
      841 cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc ctgacagcat
      901 cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag cgattaagcc
      961 cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg
     1021 tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa
     1081 ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa
     1141 tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt
     1201 agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag
     1261 ctgcatttct ggtcgttgcc tgcagttctc ctacgccgcc gcgtggcgtg acagtagtga
     1321 ataatttcga cgctaaacgc tatctcggta cctggtatga gattgcccgt tttgatcacc
     1381 gttttgaacg tggactggaa aaagtcaccg caacatacag tctgcgtgat gacggcggcc
     1441 tgaatgtcat caataaaggc tataaccctg acagagaaat gtggcagcag agtgaaggga
     1501 aagcgtactt taccggcgca ccaacacgcg ctgcgctgaa agtgtcattc tttggtcctt
     1561 tctatggcgg ttataacgtc attgcacttg atcgggaata ccgccatgcg ctggtttgcg
     1621 gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga
     1681 aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg
     1741 tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta
     1801 acgccagact cgccaggcgc atcgggttag cggactcacc gagcagcaca atgccggtga
     1861 tggccgcgcc gactgcgcca atacccgtcc acacggcata agccgtccct accggtaacg
     1921 atttcatcgc ccaggcaagt agcgccatac tgactatcat cgccgtcaca gtaataacac
     1981 ttggcgtcaa acgactaaag ccgtgggtat atttcaggcc aacggcccat accacttcca
     2041 gcagaccagc aataactaag ataatccagg acatatcagg ctccggagaa ttggggccat
     2101 ccccggtgaa aagaggcgtt tgcaggtcgt cctgcaaagc tgatggttga acggatattt
     2161 tgccaggtgg caggggaatt tcaactatta cctttgtgac tatcgcaaaa aaacaaacgg
     2221 cacgacataa ctgtcgtacc gttattgctt attgctgcgc tttcgttgct gcaccagaaa
     2281 tcgcgttacc gccatcagaa atgtcttcac caacgccacg cgtggtgttg caggcagtta
     2341 atactgttga aagcaccaga acagaaaaga tcgctgcaat tgtcttcttc accataacgt
     2401 cttcctttta ggtaatgtta tttatgtttg cctatagcaa attaataata gacaacatcg
     2461 ccaaaaatga tggaaatctg atgatttttc ggaagagaag aaaaattagc tggcagcgcg
     2521 ggagatggag ttgccgagat gtttgatgtc ttcgccgaaa ccgcgagcgg tgttacagcc
     2581 cgtgagcagc gtgctggcaa gcaaaacaag aacgataagg cgtttcatca tctctgacct
     2641 gaataagtga tggtgcagcc tgcaggccgc accacaaccg cattacttca cgcgagagac
     2701 gtattcacca gagcgggtat ccactttgat gacttcgccg atttgtacga acagcggaac
     2761 tttaaccaca gcgccagtag acagggtagc cggtttgccg ccagtacctg cggtatcacc
     2821 tttcaggccc ggatcggtat caacgatttc cagttcaacg aagttcggcg gagtaacgga
     2881 gatcggctga ccattccaca gagttacgat acactctgcc tgatccagca gccatttagc
     2941 gttgtcgcca attgcttttg catcagcaga cagctgctcg aaagtttcgt tgttcatgaa
     3001 gtgccagaac tcaccgtcgt tgtacaggta agtcaggttc atatcgacaa catcagcgcc
     3061 ttcagcggaa tcagtagatt tgaaggtttt ttctacgcga gtaccggtca gcagacgacg
     3121 cagtttaacg cgagcaaatg cctggccttt acccggtttt acgaattcac tcgcttcaac
     3181 cgcgtaaggt tcgccgtcta acatgatttt aagaccagca cgaaaatcgt tgctatagta
     3241 cgttgccata aggccctctg aaatttgtta attggtagct aagccacaaa atggcgcata
     3301 ttgtaaccct aaatacccca tccagagaag attggttaac gcaacttgcc gatgttgtga
     3361 ccgatcctgg tgaacttctg cgtcttttga atatagacgc ggacgaaaaa ctgttggccg
     3421 gacgcagcgc caaaaagctg tttgccctgc gtgtgccccg ctcatttatc gatcgcatgg
     3481 agaaaggcaa tccggacgat cctattttgc gtcaggtact tacctcgcaa gatgagtttg
     3541 tcgtcgcgtc cggattctcc accgacccac tggaagaaca gcacagcgta gtgcctggtt
     3601 tgttgcataa ataccacaac cgggcgcttt tgctggtcaa aggcggctgc gcggtaaatt
     3661 gccgctattg cttccgtcgc cacttcccct atgccgaaaa tcagggcaac aagcgtaact
     3721 ggcagactgc acttgagtat gttgctgcgc atccggaact ggacgagatg attttctccg
     3781 gcggcgatcc gctgatggcg aaagatcacg agctggactg gttgctcaca caactggaag
     3841 ccatcccgca tatcaaacgt ctgcggattc acagccgtct gccgattgtg atcccggcac
     3901 gtatcaccga ggcgctggtt gaacgctttg cccgttctac gctgcaaatc ttgctggtga
     3961 atcacatcaa tcatgccaat gaggtagatg aaacgttccg tcaggcgatg gctaaattgc
     4021 gccgtgtcgg tgtcaccctg cttaaccaga gcgttctgtt acgtggtgtg aacgataacg
     4081 cacaaacgtt ggcaaacctg agtaatgcgt tgttcgatgc cggcgtgatg ccgtattacc
     4141 tgcatgtgct agataaagta cagggcgcgg cgcattttat ggtgagtgat gacgaagcac
     4201 ggcagattat gcgtgagttg ctgacattgg tgtcgggata tctggtgccg aaactggcgc
     4261 gagaaattgg cggcgaaccc agcaaaacgc cgctggatct ccagctacgc cagcagtaac
     4321 tgattaaaca gggaagttaa acattagcct gatgcgctac gcttatcagg cctacattgt
     4381 gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat
     4441 ccggcaagag caacaaacgc aagtacagca acctgtcatt aaactgggaa atttaatctt
     4501 tgcccgttgc ataaccttta att
//
LOCUS       DAZOYL010000290         4513 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZOYL010000290 DAZOYL010000000
VERSION     DAZOYL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.291"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            2..1288
                     /locus_tag="ACHUOY_003532"
     CDS             2..1288
                     /locus_tag="ACHUOY_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR0684200.1"
                     /translation="MFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDSIGPAAKA
                     MAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVNVDIWN
                     IWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKLGDWFARDTRNFFELEGIAIPHGT
                     SAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAGY
                     DVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPAL
                     LLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTL
                     ISGVIIMSITLWIATQTIGLHTQLAANAGALKAGGMVASMDQGGSPITWLLIQVFSTQ
                     NIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLAE"
     gene            1336..2376
                     /gene="gatD"
                     /locus_tag="ACHUOY_003533"
     CDS             1336..2376
                     /gene="gatD"
                     /locus_tag="ACHUOY_003533"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0684201.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            2482..3255
                     /locus_tag="ACHUOY_003534"
     CDS             2482..3255
                     /locus_tag="ACHUOY_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0684202.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(3337..4236)
                     /gene="yegS"
                     /locus_tag="ACHUOY_003535"
     CDS             complement(3337..4236)
                     /gene="yegS"
                     /locus_tag="ACHUOY_003535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR0684203.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
BASE COUNT         1109 a         1012 c         1181 g         1211 t
ORIGIN      
        1 tattttttct aaaatattag gcatgaaggc aggcgattgc tttaaagcgg gtctgcatat
       61 cgggattggc tttgttggca ttggccttgt gattggctta atgctggatt ccattggtcc
      121 ggcggcgaaa gcgatggcgg aaaatttcga cctgaatctg catgtggtcg atgtcggctg
      181 gccgggctct tcaccaatga cctgggcgtc gcaaattgcg ctggtggcga ttccgattgc
      241 gattctggtt aacgtggcga tgttactgac ccgtatgacg cgggtggtaa atgttgatat
      301 ctggaatatc tggcatatga ccttcaccgg cgcgttgctg catctggcaa ccggttcatg
      361 gatgataggg atggcaggtg tggtaattca cgcggcgttt gtttataagc tcggcgactg
      421 gtttgcccgc gatacccgaa atttctttga gctggaaggt attgctattc cgcacggtac
      481 gtcggcgtat atggggccga ttgcggtgct ggtcgatgct atcatcgaga aaatcccagg
      541 cgttaaccga attaaattta gcgccgacga tattcagcgc aaatttggtc catttggcga
      601 gcctgtcacc gtgggttttg tgatggggct gattatcggc atcctcgccg gttacgatgt
      661 caaaggtgta ttgcagctgg cggtaaaaac ggcggcagtt atgctgctga tgccacgggt
      721 gattaaaccc atcatggatg gtttaacgcc catcgctaag caggcccgta gtcgtttaca
      781 ggcgaagttc ggcggtcagg agttcctgat tggcctggat cccgcattac tgctggggca
      841 tacggcggtg gtatcggcaa gcctgatttt tatcccgctc accattttaa ttgctgtttg
      901 tgttccgggt aatcaggtac tgccgtttgg cgatcttgcc accatcggct tcttcgtggc
      961 gatggcggtc gcggtgcatc gtggaaatct gttccgcacc ttaatctcgg gtgtcatcat
     1021 tatgagcatc accctgtgga tcgcgacgca aactattggt ttgcacaccc aactggcggc
     1081 taatgctggg gcgttaaaag ccgggggtat ggtggcttca atggatcagg gcggttctcc
     1141 cattacctgg ttactgattc aggttttctc cacgcaaaat attcccggtt tcattattat
     1201 cggcgcaatt tatcttaccg gtattttcat gacctggcgt agagcgcgtg gttttattaa
     1261 acaagagaaa accgttctcg cagaataatt tttacctgag ggggaggtta tcccccttaa
     1321 caatcaggag tttttatgaa atcaatggtg aatgatactg atggtatcgt gcgcgttgca
     1381 gaaagcgtca ttcctgaaat taaacatcag gatgaggtgc gggtaaaaat tgccagctcg
     1441 ggcttatgtg gttccgattt acccaggata tttaaaaatg gtgcacatta ttatccaata
     1501 acgttaggcc atgaatttag cggctatatt gatgcggtgg gatccggtgt tgatgattta
     1561 caccctggcg atgcggttgc ctgtgtgccg ttattaccct gttttactta tccagagtgt
     1621 ttgaaagggt tttattccca gtgcgcaaaa tatgatttta ttggctcgcg gcgtgatggt
     1681 ggatttgctg aatatattgt cgttaagcga aaaaatgtct ttgctctacc cacggatatg
     1741 cctattgagg atggggcttt tattgagccg attaccgttg gcctgcatgc ttttcattta
     1801 gcgcaaggtt gtgagaataa aaacgttatt attattggtg ccggaaccat tggcctgctg
     1861 gctattcagt gcgctgtcgc gctgggagca aagagtgtga cggcgatcga cattagctca
     1921 gaaaaactgg cattggcaaa atctttcggt gcgatgcaaa catttaacag tagcgaaatg
     1981 agcgcgccgc aaatgcagag cgttttacgc gaactgcgct ttaatcagct tatcctcgag
     2041 acggctggcg taccgcaaac tgtcgaactg gcggtagaga ttgccggtcc tcatgcccaa
     2101 ctggcgctgg tgggcacgtt gcatcaggat ctgcatttaa catcggcaac gtttggcaaa
     2161 atattgcgta aagagctgac ggttatcggc agttggatga actattccag cccttggccg
     2221 gggcaggagt gggaaacggc gagccggttg ctgacagaac gtaagttaag cctggagcca
     2281 ttaatcgctc accgtggaag ctttgaaagc ttcacccagg cggtgcgtga catcgctcgt
     2341 aatgccatgc cgggcaaagt gttgctcatt ccatgaaacc gcgggccagc gtgatgctgg
     2401 cccggtattg tgcaaaacag attattcacc aatggtcccc cttcgtttac actagccaca
     2461 attgaatatg ggtaaatgac gatgaattca ttcgagcgaa ggaataagat catccaatta
     2521 gtgaatgaac agggaaccgt gcttgttcag gatctggcgg gagtatttgc tgcctcggaa
     2581 gcgacaatcc gtgccgattt gcgctttctc gaacaaaaag gcgtggttac gcgctttcat
     2641 ggcggtgcgg cgaaaataat gtccggtaat agtgaaaccg agacccagga agtcgggttt
     2701 aaagagcgat ttcagctcgc cagcgcgcca aaaaacagaa tagcgcaggc ggcagtcaaa
     2761 atgatccatg aagggatgac cgttattctc gacagcggaa gtacgacaat gcttatcgcc
     2821 gaaggattaa tgaccgctaa aaatatcacg gtgattacca acagtctccc ggcagcgttt
     2881 gccctttccg aaaataaaga cattactctg gtcgtctgtg gtggcacggt ccgccataaa
     2941 acgcgctcga tgcatggttc cattgccgag cgttcattgc aggatattaa tgccgattta
     3001 atgtttgtcg gtgctgatgg tattgatgcg gttaatggta ttacgacctt taatgaaggt
     3061 tattcgatta gcggtgcgat ggtcaccgcc gctaataaag tgattgccgt tctcgattca
     3121 tcgaaattta accgtcgcgg ttttaatcag gtactgccaa tcgaaaaaat tgacattatt
     3181 attaccgatg atgctgtatc agaggtggat aaactggcat tgcagaagac acgggtaaaa
     3241 ttaattacgg tgtagtattg ttctgtatgc cggataaggt atttacaccg catccggcat
     3301 ttcggattct ccctggttca agagagaata gtttgattaa cgcaataata gacaatctgg
     3361 tggtaatcga caacgcaacg ccgccggaag tatttcaata tgaaagtttt gtccgctcaa
     3421 cggttcgcca tcaagattaa aagtgatttc gtgtggggct tgtatatcaa accacgacga
     3481 agcgccttcg ataatattcg ggttatcttc gtcagatttt aaggttgata cgagagccgg
     3541 aagaatttca tcgccggtaa aaatgcgcag ttgcagcaag ccatcgttaa ttaacgcgtt
     3601 cggacacaat tgctgaccgc caccggcctg acgcccgtta ccaataccaa tgaccagggc
     3661 gtcaccttgc cagtgaaagt tttcaccgcg gatttcacaa cggtccggtt gcagagtatc
     3721 catgcgcatt aagccatgaa tgatgtaaga gacgccaccc agcgcggctt ttaatttttc
     3781 cggcgtttct gtggtaatac gcgtcccaaa tccgcctgtc gccatattaa taaaacaggt
     3841 ttgtttgttg acctgcgcca tatctatcgc aatggcgtta ccggcaattg ccagtttcag
     3901 cgccttatcc agtgcctcag gaatccctac actggtggca aaatcattgg cggttcctaa
     3961 tggcaaaatt cccagcgcgg gtatgccatc cccctcacac tgaatcaacg ccgtagaaac
     4021 ttcattaatg gtgccatcgc caccaccggc aatcaccgtt gcgacgccca acttccgggc
     4081 ctcctctaca tatcgtgcgg catcgccttt ctcccaggtg acccgcacat ggatcgtcat
     4141 tccttcctca cgcaacagca taattgcttc gcgcaagggt agattgtcag tacttttgcc
     4201 attaagaatc agtaagctgg cgggaaattc tgccatgatc gtatgtgcct ttatgattgg
     4261 tctgtagata gtgtagagca gaaaacaaaa ggtggagtca gaacaggatg aaagtcggag
     4321 gataaaaagt cagttcaagt atggaggatt cagtggttac caccaggagc ttgcaatggg
     4381 gacagcgaat actaacggct gccaccgata aatttcaaaa aagagcatat acctaatatt
     4441 caactaaaca gtggcatctt caatataata tattaaagcc cccatggagt taccctgaag
     4501 ggcctcaatg tcc
//
LOCUS       DAZOYL010000291         4482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZOYL010000291 DAZOYL010000000
VERSION     DAZOYL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.292"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(7..1068)
                     /locus_tag="ACHUOY_003536"
     CDS             complement(7..1068)
                     /locus_tag="ACHUOY_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR0684204.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="ACHUOY_003537"
     CDS             complement(1065..2462)
                     /locus_tag="ACHUOY_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR0684205.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="ACHUOY_003538"
     CDS             2617..3615
                     /locus_tag="ACHUOY_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0684206.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHUOY_003539"
     CDS             complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHUOY_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR0684207.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1098 g         1063 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg cc
//
LOCUS       DAZOYL010000292         4482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZOYL010000292 DAZOYL010000000
VERSION     DAZOYL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.293"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..2252
                     /locus_tag="ACHUOY_003540"
     CDS             <1..2252
                     /locus_tag="ACHUOY_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0684208.1"
                     /translation="KLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSVGKT
                     TSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMSGFGR
                     VNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEGINAL
                     AGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSIGVM
                     AAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSARTYE
                     NKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSAQNRS
                     DALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPFAPSG
                     QQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDDKEGG
                     FNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAETWQAG
                     FNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDWEGIS
                     DEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTYFGAG
                     DMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQDLPQ
                     IPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSARGRT
                     WIFGGDIRF"
     gene            2321..3496
                     /gene="senB"
                     /locus_tag="ACHUOY_003541"
     CDS             2321..3496
                     /gene="senB"
                     /locus_tag="ACHUOY_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR0684209.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKK
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(4022..>4482)
                     /locus_tag="ACHUOY_003542"
     CDS             complement(4022..>4482)
                     /locus_tag="ACHUOY_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR0684210.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
BASE COUNT         1252 a         1044 c         1048 g         1138 t
ORIGIN      
        1 taaaactggc tatcggctca ggcatattat tgctcagctg cggtgcttac tcacaatcca
       61 tcagtgaaaa aactaattcc gacaaaaaag gagcggcaga attcagtccg ctcagcgttt
      121 ctgtcgggaa gacgaccagt gagcaggaag ctctcgagaa aacaggcgcg accagttccc
      181 ggacaacgga caaaaacctg caatcacttg acgcaacagt gcgtagtatg cctggtactt
      241 atactcaaat agatcctggt cagggagcaa tcagtgtgaa tattcgaggc atgagcggat
      301 ttggtcgtgt aaacactatg gtcgatggta ttacccagag tttttacgga acctctacct
      361 ccggaacaac gacgcatggt tcaactaaca atatggctgg cgtacttata gatcctaact
      421 tactggtagc agttgatgtt acacgcggtg acagcagtgg ctctgaaggg atcaacgccc
      481 ttgccggtag tgcaaatatg cgtactattg gcgttgacga tgtaatattt aacggtaata
      541 catatggcct tcgttcacgt ttctctgtcg gtagtaatgg gctgggacgc agcggaatga
      601 tcgcccttgg tggaaaaagc gacgctttta cggatacggg aagcattggc gttatggctg
      661 ctgtgagcgg cagttctgtg tactctaatt tctcaaatgg ttctggaatt aacagcaaag
      721 agtttggtta tgataaatat atgaagcaga accccaaatc ccaactgtat aaaatggata
      781 tcagaccaga cgaatttaac agcttcgaac tttccgctcg aacctatgaa aataaattta
      841 cacgtcgtga tataaccagt gacgactatt acattaaata tcattacacc cctttttctg
      901 aattaattga ctttaacgta acggccagta ccagtcgcgg taatcaaaag tatcgtgatg
      961 gctcgctgta tactttctac aaaacctcag cgcaaaatcg ttctgacgcg ctggatatca
     1021 acaataccag ccggttcact gtcgcggaca atgacctgga gtttatgctg ggcagcaaac
     1081 tgatgcgtac ccgctatgac cggaccattc actcagcggc gggcgacccg aaagcgaatc
     1141 aggaatcgat cgagaacaat ccgttcgcac cctccggcca gcaggatatt tcagcgctgt
     1201 ataccgggct gaaggttacg cgcggcatct gggaggcaga tttcaatctc aactacacac
     1261 gtaacaggat cacagggtac aagcccgcct gcgattcacg cgttatctgc gtgccacagg
     1321 gtagctacga tattgacgat aaagagggtg gcttcaaccc ttcagttcag ctttctgctc
     1381 aggtaacacc atggcttcag ccgttcattg gctacagcaa atccatgcgc gccccgaaca
     1441 tccaggagat gttcttctct aattcaggag gcgcatccat gaacccattc ctgaagcctg
     1501 aacgtgcaga aacctggcag gcgggtttta acattgatac cagagattta ctggtcgaac
     1561 aggatgccct gcgctttaag gctctggcgt accgcagcag gatccagaac tacatctaca
     1621 gcgagtctta tctggtttgt tctggaggtc gtaaatgcag tatgcctgag gtgattggca
     1681 atgactggga gggcattagc gatgaataca gcgacaatat gtacatctac gttaactcgg
     1741 caagcgacgt tatcgcaaag ggcttcgaac tggagatgga ttatgatgca ggttttgctt
     1801 ttggccgact ctctttcagc cagcagcaaa cagaccagcc aacctccatc gccagcacct
     1861 actttggcgc aggggatatg accgaactgc ccagaaaata catgacgctg gatactggtg
     1921 ttcgcttctt cgataacgcg ttgaccctgg gcactatcat aaaatacaca ggcaaggctc
     1981 gtcgcctgtc gcctgatttt gagcaggacg aacataccgg cgcaataatc aaacaggatt
     2041 tgccgcagat cccaacgatt atcgatctct atggtactta cgagtacaac cgcaacctaa
     2101 cactgaaact ttcggtacaa aacctgatga acagagatta ttcggaggcg ctgaataagc
     2161 tcaacatgat gccaggtctt ggtgacgaga cccacccagc caattccgcg cgtggcagaa
     2221 catggatatt tggcggggac attcgtttct gatgacaatt tccgctaggt tcagacaata
     2281 cgttttctct cttatgtcaa ttttattgca ggaacgaaaa atgaatattt tcactttatc
     2341 caaagcaccg ctatacctgt taatttcact atttttaccc acgatggcca tggctatcga
     2401 tccacctgaa cgcgaacttt cgcgatttgc cctgaaaacg aattaccttc agtcccctga
     2461 tgaaggcgtc tatgaactgg cgtttgataa tgccagtaaa aaggtgtttg cagcagtcac
     2521 cgatcgtgta aatcgtgaag ccaataaagg ctatctgtat tcgtttaatt cagattcgct
     2581 gaaagtcgaa aataaataca cgatgccata ccgggcattt tcgctggcga taaatcagga
     2641 taaacatcag ctctatatcg gacacaccca gtcagcgtcc ctgcgtatca gtatgtttga
     2701 caccccaacc ggcaaactgg taagaaccag cgacaggtta agttttaaag cggcaaacgc
     2761 tgcagattcg cgttttgagc attttcgcca tatggtttac agccaggatt ccgataccct
     2821 gtttgtgagt tatagcaata tgctgaaaac ggccgagggc atgaagcctc tgcataagct
     2881 gttaatgctc gacgggacga cgcttgcctt aaaaggcgag gttaaggatg cttacaaagg
     2941 tacagcgtat ggtctgacga tggatgaaaa aaaacagaaa atctacgttg gcggaagaga
     3001 ttacatcaac gaaattgatg cgaaaaatca gacgctgctg cgtaccatcc cgttgaaaga
     3061 tccgagacca caaatcacaa gtgtgcagaa tctggcggtg gactccgctt ctgaccgtgc
     3121 ctttgtggtg gtattcgacc atgacgatcg ttccggtaca aaagatggac tctatatttt
     3181 tgacttacgc gacggtaaac agcttggcta tgtgcacaca ggagccggag ctaacgcggt
     3241 gaaatacaat ccgaaatata acgaactgta tgtcaccaac ttcactagcg gcaccatcag
     3301 cgtagtggat gccaccaaat acagcatcac ccgtgaattt aacatgccgg tctacccaaa
     3361 ccagatggtg ttgtcggacg atatggatac cctttacatt ggcatcaaag aaggctttaa
     3421 ccgcgattgg gatcctgatg tgtttgtgga aggagctaaa gaacgtattc tgagcattga
     3481 tttgaaaaag tcgtgatcgg ggggaaaaaa catgggataa aaactccgtt attccactaa
     3541 actggccgga ctaggaaaag caaacatctc tgtcctgccg gtacgcctgt tacgccgctc
     3601 catgaggctg aggaatctcc agaaaacgag acaggcacag cattgtacga tcaactgatt
     3661 tgtgccaata attaaatgaa atcacagcta tgcctgttca tggggttcta ggccggaacc
     3721 ccagattttt ccgattgttt gttttctaac tgattattca gtagttttag agggctgacg
     3781 ctcagtggaa cgaaaactca cgttaagagg ttttgactat gataaaagtc aaagtagggg
     3841 tgagtttgga tatggaaatt attgtactat aaggagagca tgaaaacccc tggggcagtc
     3901 actgaatgag ggaccagcag cggtaaaata accagcataa tttctgcatg gtcgattttt
     3961 ataccgtgac aggctgtttt ttatacttgg ggtcagtttg gatatcgaaa attattgtac
     4021 gttaaggttg ttttttgacc ttgtcgatct taactgatct caaacgacga aacttacctg
     4081 aagtcggtat ccggctgttt ttccagatat aatcgcctgt cagattgata tgttcccaac
     4141 ctaatggtga taaatgcgat aatagttgag catttatttt catgccctta cgcttcagag
     4201 catcaaccgc tctttccatg taaactgtgt tccataagga aatagccgcc gtcaataaag
     4261 tcaatccact ggcgcgataa ctttgatttt ccggtttccg atctctaatc tcccccaatc
     4321 gatgcatgaa tacagcacgg gccagagcat tacgggcttc tcctttgttc agcccggcct
     4381 ggacacgtcg ccgcaatgcc ggatcccgaa accagtccag cataaacagt gttcgttcta
     4441 tacggccaat ctctctcaga gctttagcca acccattttg tt
//
LOCUS       DAZOYL010000293         4459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZOYL010000293 DAZOYL010000000
VERSION     DAZOYL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.294"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(123..758)
                     /gene="msrQ"
                     /locus_tag="ACHUOY_003543"
     CDS             complement(123..758)
                     /gene="msrQ"
                     /locus_tag="ACHUOY_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR0684211.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            complement(759..1763)
                     /gene="msrP"
                     /locus_tag="ACHUOY_003544"
     CDS             complement(759..1763)
                     /gene="msrP"
                     /locus_tag="ACHUOY_003544"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR0684212.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1872..2285)
                     /gene="hiuH"
                     /locus_tag="ACHUOY_003545"
     CDS             complement(1872..2285)
                     /gene="hiuH"
                     /locus_tag="ACHUOY_003545"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR0684213.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2418..3089
                     /gene="hprR"
                     /locus_tag="ACHUOY_003546"
     CDS             2418..3089
                     /gene="hprR"
                     /locus_tag="ACHUOY_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR0684214.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3089..4447
                     /gene="hprS"
                     /locus_tag="ACHUOY_003547"
     CDS             3089..4447
                     /gene="hprS"
                     /locus_tag="ACHUOY_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR0684215.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
BASE COUNT         1241 a          944 c         1056 g         1218 t
ORIGIN      
        1 ttatgttgat aatttgaggt tgatgacaaa cgaaacataa cctgatgtga tctgcgcagg
       61 ccggacaggg cgctcgcgcc acatccggca gggataacgc gtactttgtc ggcaatatga
      121 agttatacag ataatttgtt gtgaacctgc ttgcgtaacc gcttaaaaag ggaacgcaac
      181 ttcttatacc gtaaggctaa aagcagtaca gccagcccag cgtagatgag cggctgcggt
      241 gaaataatct tcactgacca cagataatgt attggggcca ggatcgcgac aagatagacg
      301 aaattatgca actgttgcca gtgcttgccg agttttcgct gcatcgcctg ggttgaagta
      361 aacgctaaag caagcaaaat aacccagctg ataattccta acgttaaata aggccgggta
      421 attaactcct tgcctaataa cgccagatta ttcacgccca gctccagcaa tgcgtaactg
      481 gttaaatgca gtgtcgccca ggcaaagcac cataagccta acaggcggcg agtgcgtatc
      541 aataacggct gttttgcgta gcgtgccaga ggtgtgatta acaaggtcgc cagcaaaaat
      601 ttcagtgcag tgcgaccggt aaaatgctga atatctttca ccggatcggc acccagtcca
      661 ccgtgattga tcgcccagac cagccagaga aacggcaaca atccggcaag atgcaggcta
      721 actttcagcc atgtcacctg ttttgctgtc agacgcattc agaaattctc ccgcaaatcc
      781 aggccacgat acagcgatgc cacctggtcg gcgtaaccat taaacagtag cgttggctgg
      841 cgctgaacat cgaggatgcc gcctgaacca ataaatcgtt cggtagcctg tgaccagcgc
      901 gggtgatcaa catgcggatt aacgttggcg taaaaaccgt attcgtcagg cgctgccaga
      961 ttccaggtgg ttggcggacg ctcgcgggtc agcttaatac tgactatcga tttaatccct
     1021 ttaaagccat atttccacgg cacaatcagt cgtaccggcg cgccattttg tggcggtaac
     1081 gccttgccat aaacacccac ggtcatcagt gtgagcggat gcattgcttc gtcgagacgc
     1141 aatccttcga cataaggata tttcagcccg ccgccgataa agcggtcctg ctggcctggc
     1201 atttgttccg gtgcataaat tgtttcgaaa gcgacatact tcgcattgct ggtgggttcg
     1261 gcaagcgcca gcaatttgtg cagcggaaaa ccaatccacg gcaccaccat cgaccatgcc
     1321 tccacgcagc gcatacgata aatacgctct tccagcggga agcgacgggt taaatcatcg
     1381 tgatcgaggg tcaatggttt tgccacttcg ccgctgattt tcagtgtcca tggatcggtt
     1441 ttcaggctac cagcattagc ggcgggatcg gctttatcca gcccgaattc atagaagttg
     1501 ttataaccgg agactttatc tactggcgtc agtggcagat tattttgcca ggcggtaggc
     1561 ttgctgaact ccagcggttt tccggcgggg gcgggcgggc gatcgttccc tttaaaccag
     1621 ctaagcagat cggcatgcgc agcgtgaggc aaagaaagtg cagctgcgct gatgcccagt
     1681 gcttttaaca cctgtcgacg cttcataaag aataccgact cggccgtaac atctgattct
     1741 tttaaaaatt gattcttttt cataagccat cacacttttt tgtcgttagc cagcaagagc
     1801 cgtttactgt taatctgtca gggagtgtcc tccctaacag tagcagagat ctgcttttac
     1861 atatttatgc attaactgcc acgataggtt gaatacccat attggctaag taataaaggc
     1921 acatgataat gctcgttcac tttattaata tgaaattcaa cggggatctc agggaagaaa
     1981 ctttcaagat tttgtttctt gaaatagtcc ccggttttaa atacgacacg gtaatcgccc
     2041 gtagttgcag tttgctcggg ccacagtgcc ttaattcgtc catccttatc tgttttggcg
     2101 gtattaagtt gtaaccagcc gttgtccgct ttcttttcaa gagtgactgt cacgtcggca
     2161 gcgggttttc cggtttgctg gttcaaaatg tgcacgctaa gaatgttttg ttgtgcggca
     2221 taaaccaaag aaggtaatga aaatgctgcc gttactacgg agagtactaa ataacgcttt
     2281 aacatgttta tatccttgtc atgtgaatga gttttgagga tagcgaaaaa gcggccaggg
     2341 taaccttaca gcagcattac aattttgtaa tgaactaggc tgtttctata acatatgatt
     2401 tatggcatat tattttcatg aagattttac ttattgaaga taatcaaagg acccaggagt
     2461 gggtaacgca ggggctttcc gaagcgggtt atgtcattga tgccgtttct gatggcagag
     2521 atgggcttta tcttgcgctg aaggatgatt atgcattgat cattctggat attatgcttc
     2581 cgggtatgga tggctggcag atcttacaaa cgttaagaac agcaaagcaa acccctgtta
     2641 tttgccttac tgcaagggat tctgtcgatg acagagtcag agggctggac agtggagcaa
     2701 atgattatct ggtaaaacct ttttcatttt ctgagttgct ggcaagggtt cgggcacaat
     2761 taaggcaaca tcacgctttg aattcaacat tagaaatcag tggcttaaga atggactctg
     2821 taagtcaaag tgtgagcagg aacaatatca gtattacact gacgcgcaag gagtttcagt
     2881 tactttggct actggcctcc agagctggcg aaattatacc cagaacggtt attgcgagtg
     2941 aaatttgggg aatcaacttt gatagtgata ccaatacggt ggatgtcgcc attcgcaggc
     3001 tccgcgcaaa agttgatgat ccttttcctg aaaagctaat cgccacaatc cgggggatgg
     3061 gctattcatt cgtagcggta aaaaaataat gaaaagatta tctataaccg tccgtttaac
     3121 cttgcttttt atattgctgc tgtctgttgc tggcgctgga attgtctgga ctctctataa
     3181 tggcctggca agtgagttga aatggcgcga tgatacaaca ctcattaacc ggacagcaca
     3241 gatcaagcag ttgttaattg atggggtaaa tccagatacg ttacctgtat actttaaccg
     3301 gatgatggat gttagtcagg atatcttgat cattcatggt gatggcatca ataaaattgt
     3361 taaccggaca aatgtcagtg atgacatgtt aaataacata cctgctagtg agacaatcag
     3421 cgcagctggc atttacagaa gcattattaa tgatactgag attgatgctt tacgaattaa
     3481 tattgatgaa gtttcgccat cattaacggt tactgtggct aaattggctt cagccagaca
     3541 taacatgctt gaacagtata aaattaatag cattataatt tgcattgtcg ccattgtact
     3601 ttgctcagta ttaagtccgc tgttaatcag aacgggatta cgagagatca aaaagttgag
     3661 tggtgtaacg gaagcgctga attataacga tagccgggag cctgttgagg ttagcgcatt
     3721 accgagagaa ctaaaacctc ttgggcaggc gttgaataaa atgcatcatg ctttagtcaa
     3781 agattttgag cgtctaagtc aatttgctga cgatctcgct catgaactta gaacgcccat
     3841 taatgcatta ctgggtcaga atcaggttac gctcagtcaa accagaagta tcgctgaata
     3901 tcaaaaaaca attgccggta acattgaaga gctggaaaat atttcgcggt taacagagaa
     3961 catactgttt cttgcccggg cagataaaaa caatgttttg gtgaaactgg actcgctttc
     4021 tctcaataag gaagtcgaaa atttgttgga ttatcttgaa tacctttcag acgagaaaga
     4081 gatttgcttt aaggtcgagt gcaatcagca aatctttgcg gataaaattt tactgcaacg
     4141 aatgttatcg aatcttattg ttaatgccat tagatattca ccagaaaaat cgcgtattca
     4201 tataaccagt tttcttgata ccaacggcta tcttaatatt gatgtcgcca gtcctggaac
     4261 gaaaattcat gagcctgaaa aactcttccg tagattttgg cggggagata attcgcgtca
     4321 ttccgtaggt cagggacttg gtctttcttt agtcaaagcg attgccgaat tacatggggg
     4381 aagtgctacg tatcactatc tcaataagca taatgtgttc cggattatgt taccgcaaag
     4441 aaattaacat agggcttca
//
LOCUS       DAZOYL010000294         4427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZOYL010000294 DAZOYL010000000
VERSION     DAZOYL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.295"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHUOY_003548"
     CDS             complement(<1..590)
                     /locus_tag="ACHUOY_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0684216.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHUOY_003549"
     CDS             complement(583..993)
                     /locus_tag="ACHUOY_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR0684217.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHUOY_003550"
     CDS             complement(999..1532)
                     /locus_tag="ACHUOY_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR0684218.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2616)
                     /locus_tag="ACHUOY_003551"
     CDS             complement(1510..2616)
                     /locus_tag="ACHUOY_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR0684219.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLVLVLVLGENILSCDTERSIRERFSEYQV
                     SGQRPGNDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQR
                     AALTRETTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTT
                     ELRVGPADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2613..4004)
                     /locus_tag="ACHUOY_003552"
     CDS             complement(2613..4004)
                     /locus_tag="ACHUOY_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR0684220.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4051..>4427)
                     /locus_tag="ACHUOY_003553"
     CDS             complement(4051..>4427)
                     /locus_tag="ACHUOY_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684221.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT         1062 a         1295 c         1044 g         1026 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcaccagc accagcacca gcaccagcgc cgtcgccgct ttacctgttc cgggttttcc
     2101 cagaaccagt cgcccgcatt catcgtcata agccagcgtc tgaacctgcc ccagtaaccg
     2161 gttaagacaa tcggcaacgg tttcgccgtg ttccggctgg gcatcaatca ccgctgtctg
     2221 cggcacgcca gcatcaacaa cggtgatgcc aaatggcgca gccagttcac tgactattct
     2281 gagcaggttt tttccgctct gctggagtgg caaagcagag cagtcaacca gatcggctgt
     2341 tttgcttcgc ccgacaattc ccatgctgac gctgctggcg tcataacgaa gcggtagtgc
     2401 ctccacatat ccggtgagca caggctcatc cccgataagc acttcaacca gctcaccatt
     2461 ttttatccgg ggttgataat cccggcttcc gggccagcgg gtggtaatgg caacattaaa
     2521 atcccgggca atacggttaa tgcccgcact gatacggacg gatgtccagc cgccccattc
     2581 gcgaccggaa acccgaagta aaacggtatc attcatctga cgggtaccct taatgccctg
     2641 accggaacaa agcccggatg agagatggca tttcgatcca ggatatcagt ttcacgggac
     2701 gcatcgtcgt accatgacgc agccagaaca agcgcaggca gaacttccgc tggcgttcgc
     2761 tctgcggttt cctccgtctg aaccagacgc gcctgaatat cccggttcag ttctgtacgt
     2821 aatgacgtca gctgaaaaaa cagcctgtca tccgccgtac gcctgagctc ctgttcaatc
     2881 gccgcgttca gcgatttgcg tatgatggtg agattttccc gcgtgggtgg ttgcgctctc
     2941 tcatcctgtc ctgtgctggc tgccacgctg tcaagtgccg gatgagaaac gtgaataata
     3001 tcggactgac gttcagtgga accaccaaca gccacaaccg cctgctgatt tttcaccagg
     3061 cttccgggtt gtggcagtga agtgaccgcc cttgtggctt cgctgattgc cgtcgtccgg
     3121 atgacagcag ccacaaggtt tgtctgctgt ttttgcctga taaccgatgc ggaatctgtg
     3181 ggccacaccg cacgcggtgc cagtcctggg tccagcgtaa tgccggacat tgtcgttatg
     3241 gactgcacca gatcctgtgt gttatccacc agccgggttc cggcccgcca ggtatcctgc
     3301 aacgtatgca caaaatcact ggcaaccgac ggcggcatca gaatgacgga taaatcgccc
     3361 tgcagtagtc tcgctccggc agaaacagcc gaattaacca ttttgaaagc tgtctgaacg
     3421 gttcccagca tatcggtcgt ccgggcaata acatcgttct gaataaagtc cggtattccc
     3481 gcgagatcaa aatcgccgaa catgtcttca atcaactcat ccaggaatac ggacgactcc
     3541 tccagttttc tggcggttgc agctccggcg accggaaatg acagttcccc gctctcaaca
     3601 aactgaaatg aaacccggca catacggcct tcagtgccgg agtgagaaac ggtcacctgc
     3661 ccgtcaatac agccctgcat ttcaccgaac tgcggatgga tcagcgtccc cggcccggcg
     3721 gtttcaatcg ctgtaatcag cctgtcgcgc tgctctgcgt aatcatcacc aacgagatac
     3781 gcattaatcg tcagccgtcg cgtggcacgc ccgagatctt ccgtgtacgg tttgtcacgg
     3841 tttgggtatt catggacctg aacgcggcgt ccgaacgaac cctcgtcgct ctccaccgaa
     3901 aacggaacgc cacgaaatga agcgtcgtaa agattatcgc gccaattgat acctgaagat
     3961 gcagaaaaga gtgaagaaag agaaggtaaa gaaggtaagt ccatactgtc tccactataa
     4021 aaaaaccgcc aatttctggc ggcttatata ttaatcatca ttaatatctt tcatattcaa
     4081 tttgcaattt tcgtaggtca ctcccgactc gccattaaca ttaactccag ctctgatatt
     4141 cccgttttct tttacatcta caaaaaactg gcctcttggc atcttgaacg caaatgtaaa
     4201 accaacagta tctgaatcaa aatcatcatc tttatatatt gggatcaaaa atgtcatcgt
     4261 cgctggtgat gcattgtttg caaaatcaaa tacacctata cttttgccga attcataaga
     4321 agcttttctc aatataatcg caccgctggc agaaggggat gggcacgata tcctcagttg
     4381 tctgaatcca tctgtatcca agtcaacgtc attatctgca gatgttt
//
LOCUS       DAZOYL010000295         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZOYL010000295 DAZOYL010000000
VERSION     DAZOYL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.296"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUOY_003554"
     CDS             complement(<1..105)
                     /locus_tag="ACHUOY_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684222.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHUOY_003555"
     CDS             complement(173..2122)
                     /locus_tag="ACHUOY_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0684223.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHUOY_003556"
     CDS             complement(2207..2542)
                     /locus_tag="ACHUOY_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0684224.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHUOY_003557"
     CDS             complement(2542..2898)
                     /locus_tag="ACHUOY_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0684225.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHUOY_003558"
     CDS             complement(2898..4394)
                     /locus_tag="ACHUOY_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR0684226.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1313 c          970 g         1106 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcctttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAZOYL010000296         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZOYL010000296 DAZOYL010000000
VERSION     DAZOYL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.297"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(21..1967)
                     /locus_tag="ACHUOY_003559"
     CDS             complement(21..1967)
                     /locus_tag="ACHUOY_003559"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0684227.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SADMTFRVKPESLILTIFFLGLIP"
     gene            complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHUOY_003560"
     CDS             complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHUOY_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR0684228.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2369..3076
                     /locus_tag="ACHUOY_003561"
     CDS             2369..3076
                     /locus_tag="ACHUOY_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR0684229.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="ACHUOY_003562"
     tRNA            3182..3257
                     /locus_tag="ACHUOY_003562"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..>4409
                     /locus_tag="ACHUOY_003563"
     CDS             3455..>4409
                     /locus_tag="ACHUOY_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0684230.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1149 a         1162 c         1057 g         1041 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZOYL010000297         4404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZOYL010000297 DAZOYL010000000
VERSION     DAZOYL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.298"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            138..1241
                     /gene="proB"
                     /locus_tag="ACHUOY_003564"
     CDS             138..1241
                     /gene="proB"
                     /locus_tag="ACHUOY_003564"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR0684231.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1253..2506
                     /gene="proA"
                     /locus_tag="ACHUOY_003565"
     CDS             1253..2506
                     /gene="proA"
                     /locus_tag="ACHUOY_003565"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR0684232.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2621..2696
                     /locus_tag="ACHUOY_003566"
     tRNA            2621..2696
                     /locus_tag="ACHUOY_003566"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2654..2656,aa:Thr,seq:cgt)
     gene            3302..3460
                     /locus_tag="ACHUOY_003567"
     CDS             3302..3460
                     /locus_tag="ACHUOY_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684233.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         1167 a          991 c         1124 g         1122 t
ORIGIN      
        1 taaacaactg ccgctaggct tgctgatccc gcgcaacaaa acgccatgct ttgctcgcag
       61 atggttggca accgacgaca gtcctgctaa aacgttcgtt tgatatcatt tttcctaaaa
      121 ttgaatggca gagaatcatg agtgacagcc agacgctggt ggtaaaactc ggcaccagtg
      181 tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat tgttgaactt gttcgccagt
      241 gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt gacgtcgggc gcgatcgccg
      301 ccggacgtga gcacctgggt tacccggaac tgccagcgac tatcgcctcg aaacaactgc
      361 tggcagcggt agggcagagt cgactgattc aactgtggga acagctgttt tcgatttatg
      421 gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat ggaagaccgt gaacgcttcc
      481 tgaacgcccg cgacaccctg cgtgcgttgc tcgataacaa tatcgttccg gtaatcaatg
      541 agaacgatgc tgtcgctacg gcagagatta aagtcggcga taacgataac ctttctgcgc
      601 tggcggcgat tctggcgggt gccgataaac tgttgctgct gaccgatcaa aaaggtttgt
      661 ataccgctga cccgcgcagc aatccgcagg cagaactgat taaagatgtt tacggcattg
      721 atgacgcact gcgcgcgatt gccggtaaca gcgtttcagg cctcggaact ggcggcatga
      781 gtaccaaatt gcaggccgct gacgtggctt gccgtgcggg tatcgacacc attattgccg
      841 cgggcagcaa gccgggcgtt attggtgatg tgatggaagg catttccgtc ggtacgctgt
      901 tccatgccca ggcgactccg cttgaaaacc gtaaacgctg gattttcggt gcgccgccgg
      961 cgggtgaaat cacggtagat gaaggggcaa ctgccgccat tctggaacgc ggcagctccc
     1021 tgttgccgaa aggcattaaa agcgtgactg gcaatttctc gcgtggtgaa gtcatccgca
     1081 tttgcaacct cgaaggccgc gatatcgccc acggcgtcag tcgttacaac agcgatgcat
     1141 tacgccgtat tgccggacac cactcgcaag aaattgatgc aatactggga tatgaatacg
     1201 gcccggttgc cgttcaccgt gatgacatga ttacccgtta aggagctggc cgatgctgga
     1261 acaaatgggc attgctgcga agcaagcctc gtataaatta gcgcaactct ccagccgcga
     1321 aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa gcacaaagcg aaatcatcct
     1381 caacgctaac gcccaggatg ttgctgacgc gcgagccaat ggccttagcg aagcgatgct
     1441 tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt gccgacgatg tacgtcaggt
     1501 gtgcaacctc gccgatccgg tggggcaggt aatcgatggc ggcgtactgg acagcggcct
     1561 gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc gtgatttatg aagcgcgccc
     1621 gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc ggtaatgcgg tgatcctgcg
     1681 cggtggcaaa gaaacgtgtc gcactaacgc tgcaacggtg gcggtgattc aggacgccct
     1741 gaaatcctgc ggcttaccgg cgggtgccgt gcaggcgatt gataatcctg accgtgcgct
     1801 ggtcagtgaa atgctgcgta tggataaata catcgacatg ctgatcccgc gtggtggcgc
     1861 tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg gtgatcacag gtggtatagg
     1921 cgtatgccat atttacgttg atgaaagtgt agagatcgct gaagcattaa aagtgatcgt
     1981 caacgcgaaa actcagcgtc cgagcacatg taatacggtt gaaacgttgc tggtgaataa
     2041 aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa atggcggaaa gcggcgtgac
     2101 attacacgca gatgcagctg cactggcgca gttgcaggca ggccctgcga aggtggttgc
     2161 tgttaaagcc gaagagtatg acgatgagtt tctgtcatta gatttgaacg tcaaaatcgt
     2221 cagcgatctt gacgatgcca tcgcccatat tcgtgaacac ggcacacaac actccgatgc
     2281 gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt aacgaagtgg attcgtccgc
     2341 tgtttacgtt aacgcctcta cgcgttttac cgacggcggc cagtttggtc tgggtgcgga
     2401 agtggcggta agcacacaaa aactccacgc gcgtggccca atggggctgg aagcactgac
     2461 cacttacaag tggatcggca ttggtgatta caccattcgt gcgtaaataa aaccgggtga
     2521 tgcaaaagta gccatttgat tcataaggcc attgacgcat cgcccggtta gttttaacct
     2581 tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg gccgatatag ctcagttggt
     2641 agagcagcgc attcgtaatg cgaaggtcgt aggttcgact cctattatcg gcaccattaa
     2701 gaaaattaat aacttagctt aacttctcta agaaattgta tcttctgtta tcacttacaa
     2761 acgcctatac gttgttatgg gctttggtag tgtaacacgt atgggcgagt agttcccagg
     2821 ctcaacctcg taacaagttc ctttgaagct ggaagacgtg ccaccgtctc taaaccttct
     2881 accaaaactc ttagcaagat gttccacacc tggcgcggtg tccaatgatg ctacacgtcg
     2941 tgagacgttg cagaggataa acaggctaaa tgatgggaag agttttaagt gcaatagtgg
     3001 attaaagccc acctcttcaa aacctaactt gatcgggaaa gaatcaagct ctagcacggt
     3061 ttcgtccacc ggagtacaac gggtgcctga taagagaaga actgctaaga cttcatagct
     3121 tttgtgaaaa agcccttttt agtggatacg cctacggcgc ttattttttg gtataaccat
     3181 caaaggcgct ttatggctcc tattgtcttg aaaatgtatc tttacttgta ttgtgtttta
     3241 tgcgtagtct ccctgtgtag tatgtaactt gttaattttc atatgaatac agaggcacac
     3301 gatgaaaaac attgcagcaa tcaaacgtaa taaccgcaag attcacgctc gtaagttcct
     3361 gtctacgcca gaaggaaaag cctggctaga gcgtaaacag agagagaacg aagaaagaaa
     3421 actccttagt gagttgaaat ggcttagaga ggatttctga aaatctccag aacgcaacag
     3481 gacgcattct aacgctgttt tcattactcg gtatagggat aggtgatgtg tgtttaatgc
     3541 gtctgtgcgc ttgttttgtg ccttattttg cattttcata atgcggaaga gaacacggaa
     3601 aaccttaaaa ttaattattg gggtatttaa ggctattttc aggtgattta agggtattaa
     3661 acttgattta attgattaaa atataaacag ataattatgt atcttattga tttataaggt
     3721 tgtttttaag taaaggtaaa taaaaaataa atagccataa atagctataa atacatctta
     3781 atacctatat cccttccctt atggctagat tgcgagaggt gcttaacgcg attttcagcg
     3841 ccccccgatc tggaaaatcc agaatcccca aatatcagca aggaattttc ttgcgtaatc
     3901 aacatcgttt ttacctatta aatcattaca cagaaagcct ttctgtaatg tctctcacta
     3961 aactttcact aaacacgaga atgatcggtt ttatgtcgga tcagatgcta gaaacagcac
     4021 cacgtctcac aagggctgta agcgatgaaa ccagtgttta tgcgggtgca ggtcaaaata
     4081 taggccaaaa tccatttaac ataataaatg ttatatgtgc taaggaacac atataacgcc
     4141 ttgaattgat gtatcagtgg aaaagtgatg gattttttac gtaggagcac gaaagggctt
     4201 ctattgactt ttgtgtgcta tgaaatataa ttaaagtttt ttgtttttat tgtgtgatgt
     4261 ggtgggttac acaggggaat agattaaatg gtgttctgtt tatacaggtt tatttgttag
     4321 atcaacaatt tggcatttct aaacagatgt ttgaacgttg ccaaatgtct tgacaacaca
     4381 agaaaagggc ttgctatgcc aggc
//
LOCUS       DAZOYL010000298         4380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZOYL010000298 DAZOYL010000000
VERSION     DAZOYL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.299"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1326
                     /gene="xanP"
                     /locus_tag="ACHUOY_003568"
     CDS             <1..1326
                     /gene="xanP"
                     /locus_tag="ACHUOY_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR0684234.1"
                     /translation="IYRLEDRPPLPQTLFAACQHLLAMFVAVITPALLICQALGLPAQ
                     DTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVAPLIMGGTTLKTGGAD
                     VPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVMIIGLSLIQVGLTSIG
                     GGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYALAWFMG
                     MLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATSDVSEQ
                     PVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGFVVA
                     LMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILIIAL
                     SLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            1447..3156
                     /locus_tag="ACHUOY_003569"
     CDS             1447..3156
                     /locus_tag="ACHUOY_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0684235.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(3208..>4380)
                     /gene="yicI"
                     /locus_tag="ACHUOY_003570"
     CDS             complement(3208..>4380)
                     /gene="yicI"
                     /locus_tag="ACHUOY_003570"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0684236.1"
                     /translation="GLAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQ
                     WFDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCY
                     ANYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGS
                     KSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPA
                     CDYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLS
                     LPVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKA
                     TRTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT          968 a         1200 c         1174 g         1038 t
ORIGIN      
        1 atttaccgtc ttgaagatcg tccgccgctt cctcaaaccc tgtttgccgc ctgtcagcat
       61 ctgctggcga tgttcgttgc ggtgatcacg ccagcgctat taatctgcca ggcgctgggt
      121 ttaccggcac aagacacgca acacattatt agtatgtcgc tgtttgcctc cggtgtggca
      181 tcgattattc aaattaaggc ctggggtccg gttggctccg ggctgttgtc tattcagggc
      241 accagcttca actttgttgc cccgctgatt atgggcggta ccacgctgaa aaccggtggt
      301 gctgatgttc ctaccatgat ggcggctttg ttcggcacgt tgatgctggc aagttgcacc
      361 gagatggtga tctcccgcgt tctgcatctg gcgcgccgca ttattacgcc gctggtttct
      421 ggcgttgtgg tgatgattat cggcctgtcg ctaattcagg tggggctgac ctccattggc
      481 ggcggttacg cagccatgag cgataacacc ttcggcgcac cgaaaaatct gctgctggca
      541 ggcgtggttt tagccttaat tatcctgctt aaccgtcaac gtaaccctta cttacgcgtg
      601 gcctcactgg taattgcgat ggcggccgga tatgcgctgg cgtggtttat gggcatgttg
      661 ccagaaagca acgaaccgat gacgcaagaa ctgattatgg tgccaacgcc gctctattac
      721 ggtcttggca ttgaatggag tctgctgctg ccgctgatgc tggtctttat gatcacttcg
      781 ctggaaacca ttggcgatat cacggcgacc tctgacgttt ccgaacagcc agtgtccggt
      841 ccgctgtaca tgaaacgcct gaaaggcggc gtgctggcaa acggcctgaa ctcgtttgtt
      901 tcggcggtgt ttaacacctt cccgaactcc tgcttcgggc aaaacaacgg agtgatccag
      961 ttgaccggtg ttgccagccg ctatgtcggt tttgtcgtcg cgctgatgtt gatcgtgctg
     1021 ggtctgttcc cggcagtgag cggttttgta caacacattc cagaaccggt tctgggcggc
     1081 gcaacgcttg taatgtttgg caccatcgcc gcctccggtg tgcgtatcgt ttctcgtgag
     1141 ccgctgaacc gtcgggcgat tctgattatc gcgctgtcgc tggcggttgg tctgggcgtg
     1201 tctcagcagc cgctgatttt gcagtttgcc cctgaatggc tgaaaaacct gctctcctcc
     1261 gggatcgccg cgggcggtat tactgccatc gtgctgaatc tgattttccc accagaaaaa
     1321 cagtaattca ctggcggcgg taagataatt accgccgcag tctcacctct ttaaccattc
     1381 ccgccttgag gattgcgcgt aaatcgtgca taactccctt atgtgcattt cacgggatgg
     1441 aagaccatga aatttattgg gaagctgctt ctctacattc tcatcgctct gttagtggcg
     1501 atcgctggcc tctattttct tctgcaaacc cgctggggag cagaacatat cagcgcatgg
     1561 gtttccgaga atagcgacta tcatctggcc ttcggggcga tggttcaccg tttttccgcg
     1621 ccatctcata tcgtgctgga gaatgtcacg tttggtcgtg atggccagcc cgcgaccctg
     1681 gtggccaaaa gtgtcgacat tgcgctaagt agtcggcaac tgaccgaacc acgccatgtc
     1741 gataccatcc tgctggaaaa cgggacgctg aatctcaccg accagaccgc gccgctaccg
     1801 ttcaaagccg atcgtctgca actgcgtgat atggcgttta atagcccgaa tagcgaatgg
     1861 aaactgagcg cgcagcgggt aaatggcggc gtggttccgt ggtcaccaga agccggtaaa
     1921 gtgctgggta cgaaggcgca gattcagttt agtgccggat cgctttcgct caatgatgtt
     1981 cctgccacca atgtactgat tgaaggcagt attgataacg atcgcgttac gctgactaac
     2041 ctgggtgccg acatcgcgcg cgggacatta accggaaacg cgcagcgtaa cgccgacggc
     2101 agctggcaag tggaaaacct gcgcatggcg gatatccgtc tacaaagcga aaaatcgcta
     2161 accgacttct ttgcgccatt acgctctgtc ccgtcgttgc agattggtcg cctggaagtg
     2221 atcgatgctc gtttgcaagg tccggactgg gcggtgaccg acctcgatct cagcttgcgc
     2281 aacatgacct tcagtaaaga tgactggcag acacaagaag gcaaactgtc gatgaacgct
     2341 agcgagttca tttatggttc gctgcattta tttgacccga ttataaacgc ggaattttct
     2401 ccgcagggcg tagcgctgcg ccagttcacc agccgctggg aagggggtat ggtcagaacg
     2461 tcagggaact ggctgcgtga cgggaaaacg ttgatccttg atgatgcggc aattgccgga
     2521 ctggaatata ccttgccgaa aaactggcaa cagttgtgga tggaaacgac acccggttgg
     2581 ttaaacagcc tgcaactgaa gagatttagc gccagccgca atctgatcat tgatatcgac
     2641 cctgacttcc cgtggcagct caccgcgctc gatggttacg gtgccaacct gacgctggtt
     2701 accgatcata aatggggcgt ctggagtggc tcggcgaatc tgaatgccgc cgccgcgacg
     2761 tttaatcgtg ttgatgtccg tcgtccgtcg ctggcgctaa ccgccaacag cagcacggtg
     2821 aatatcagcg aactgagtgc atttactgaa aaaggcattc tggaagccac cgccagtgtt
     2881 tcacaaacgc cacaacgtca gacacatatc agcctgaatg gacgcggtgt gccggtgaat
     2941 attttgcaac agtggggatg gcctgaatta ccgttgactg gcgacggcaa tattcagctt
     3001 accgccagtg gcgatattca ggccaatgtc ccgttgaaac ctacggttag cgggcaattg
     3061 catgccgtga acgccgcaaa gcagcaagtg actcaaacca tgaatgcggg cgtcgtttcc
     3121 agcggtgaag ttacatcggc agagctggtg cagtaaacat caatctcgcc gggtacgact
     3181 cagacgtacc cggcattctt caatagatta caacgtaatt gtcagcgcat tcccttgagg
     3241 cgtaaccacc agcccctgct cactttcagc ctgcgaaccg ccttgcagac catttacttt
     3301 cacaatattg cgcaagcaca gtgtccagtt cctcgcctcg cccgcaccag tcacagtaat
     3361 cgtgttacca gtacgtgttg cttttagagt aaagatcacc gaaccgtcag cagcgggcac
     3421 ttcacataca gcttcatgcc cgtcttgcag attaaagaga tggaatgccg tgccttcgtg
     3481 ccacgcgtaa tcgggacgtt gatcgttgtt gcccagcgcc aatagggtgt tatcacgcac
     3541 ataaacgggc agactcagga agccgtgctg ctgtttatgc cagcgactac cgtcgagctc
     3601 attgttgtgc cacaggtgtg tccagcgacc ttccggcaag tagaactgca cctcgcccgc
     3661 ctcagtgaac accggagcaa ccatcacgtt gtcgcctagc atgtattgac ggtcaaggta
     3721 atcacaagcc ggatcgtccg ggaactccat catcatggcc cgcatcatcg gcgtaccccg
     3781 cgctttcgca cgcgcagctt cacgatacag atacggcatc atgcggcatt tcagttgcgt
     3841 gaagaagcgc accacatcac aggactcatc atcgtaggcc cacggcacac gataagattt
     3901 gctgccatgt aaacgactat ggctggagag caaaccaaac gcgcaccagc gtttgtaaac
     3961 gtgtgccggc gcggtatttt caaagccgcc gatatcgtgg ctccagaagc caaaacctga
     4021 aaggccaata gacaaaccac cgcgcaggct ttccgccatt gattcgtagt tagcgtaaca
     4081 atcgccaccc cagtgtaccg ggaatttctg cgcaccgacg gaggccgagc gggcaaacaa
     4141 gacagcttct tcctcaccaa cggtgtcctt gagcacgttc cacaccagtt cgttgtagat
     4201 gtacgcataa tggttatgca ttttctgcgg atcggaaccg tcaaaccact gaacatcagt
     4261 tgggatacgt tcgccaaagt cggtcttaaa gcaatcaacg cccatcgcga ccagaccttt
     4321 cagtttgtcg gcgtaccatt tgcaggcatc cggattggta aagtcataaa tcgccagacc
//
LOCUS       DAZOYL010000299         4330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZOYL010000299 DAZOYL010000000
VERSION     DAZOYL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.300"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..583)
                     /locus_tag="ACHUOY_003571"
     CDS             complement(<1..583)
                     /locus_tag="ACHUOY_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0684237.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(646..1095)
                     /locus_tag="ACHUOY_003572"
     CDS             complement(646..1095)
                     /locus_tag="ACHUOY_003572"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR0684238.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHUOY_003573"
                     /pseudo
     CDS             complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHUOY_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1029 a         1090 c         1168 g         1043 t
ORIGIN      
        1 tgaagttcgg gatcatcgcg tcaacggtga tggcgtcagc aggctgtacg ccgccaacca
       61 gcgccgtacc tgccagtaaa atgtaggaga gcgttaacag cagcatcagc gttgaagtga
      121 cggaggtaat tggccccaac attttcgccc cgttggtgga aacccaggtg gagaaggcga
      181 acagcaccat actgataatg gtggtggcaa ccggcgtcat aatatattcg tatccgagga
      241 aggcatacga agcataagca ataacgcgcg gcaacagtga ggtgaaaaag aacaggttta
      301 cgaaccagta ggtataggca gtaataaatg cccaacgtcc gcccagcgaa ctttttaccc
      361 atgcgtagac accggcttct gagtttttat ttaacgaaac aaattctgcg atgatcagac
      421 agaagggaat aaaataaaaa atcgtcgcga ggaaaaacat cggtgccgag gccagtccaa
      481 gatcaatatt attattgata acgttgttaa agctgtaaac ggcggcaaaa gtcagcgaga
      541 gcaagccgaa tttgccgatt gtattacgtt tggtatcaga cataacacat ctccgaaaat
      601 gagtagggta gcggtggcgg caaactcctt ttgccgccgt agttgttatt tgttatggaa
      661 ataaccatct tcgatggtga ctttgagaac cacttttttg accgcgttat tgcagataaa
      721 ccgatacgct tcatggatat cgcaaataac gatttgccct tcgtggacct caacggtttc
      781 tccgcagcct tttaaatatt cacggtcagt ttcatcacga taatattcca ctacctgtaa
      841 tgtctctttc ggcgcatatt caattttttg ctgcccttgc aggtagtaat gcacttcaaa
      901 atagcgtcga tgcccggtaa atagcgcatc ggtcgcggag tctgtctcca cgcggtaagt
      961 caatgagtca ccgatggagt gggcgacgcc aggctgaatg ttgtcgatat tttcaatcgc
     1021 ttcaacgcag cgttgccact tcttgccaga ggcgtaaatc tggcggaact gttctaagtt
     1081 atcgatgatc ctcattgctt attctcgctg tgcaaattcg tggaaaagaa ccctgcgccg
     1141 aactcatacg acgccaggct ttgcgcggta gcttctccgc cagaaaccgg cagcaacgta
     1201 aagccgtagc tgaagtcacg gaaccagacg cgccaggagt ccagcacctc gctgccccag
     1261 gagttggagc cgaggccaag cagctggtga tcgaggttca gggtgatgtc atcactgcgc
     1321 tgcagctcgt tacagtgctg ggcagcgtgg atgttttcct aggtatagtg ccaggcgctg
     1381 aagttaattg ggcgctgcgg aaccaccagc agaccgttac cgtggcggtt agtcagtgcc
     1441 gtccagcgga catgctgacg gttaccgttg ttctgcggga agggatagtt ctcgaacatg
     1501 gcatcgacgg tgctgcgcca gatatcgatg atgttagcct gctggctgtc ggcgtagttt
     1561 tcgcccggtc cacgaccgta atacgccacc tgatcgtatt cgccgttaat tcccatggtg
     1621 aaaccgatgc acggaatgat gtgcggatag tcgccgtaac gctcgccgga aagcgccacg
     1681 ttaacctggc catcggcagc gatgcgccag atgtaggtgc agcgcattcc gaaatcaaac
     1741 accggcgggg caataaccgt gcggctgatg atcaacactt caccctcgct ctgttctacg
     1801 gcaaagtcgc gcagatgttc ctgcatgatc tgcaaatgat tcggctgcca caacccttcg
     1861 tactcctgct tgtggttgtc gatcatcggc ttgaagaagt tgatctttgg ctcgcgggtc
     1921 agcagcgact cgccatttac ctgccaggat gtcggtttgc cactcatttt tgagaaggtg
     1981 atcgcgaagt tgtagccgcg aacggtgcag ctcaaacgat cgtcttccag cgtcagcgga
     2041 cgcgcattat ttggtgcgaa aggcactggc tgcgcggtgt tttccttcag cgggaactga
     2101 taagtggcga tagaatgccc ggcttcgctg tagcgggtgc gggaatcttt ggtcaccgta
     2161 atgttgagga acgcttcgcg ggcgtccagc tgcggcagcg tgatctgcaa gggggcttcg
     2221 ctgttcggcg caacgtcgcg cagtttaatc tgctgcgtcg cgagcgtttc accttcggcg
     2281 cgcacctctg cgtgcagggg gtagtcatca agcgtggtaa accacagttt attttcgact
     2341 ttcaactcgc cgcgagtcag atcccgcgcg tggattttta ccggcgcgat aacctgtttg
     2401 tactctttca ggcccggtcc cggcgtctga tcggaataga tcaaaccatc aagacagaag
     2461 ttatagttgt tgggatagtc gccgtagtcg ccgccgaatt tataccagac attgccgtgg
     2521 tcgtcctgtg cctggatccc gtggtcgcac cactcccaga cataatgacc ctgaatgcaa
     2581 tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca
     2641 tgagcatatt cacagatgat gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc
     2701 agcggcacgc gggtgtacat ggtggaaata atatcgacca cttcagcatc gcgatcttct
     2761 tcgtaatgca ccagtcgcgt gtcatccagc gccttcgctg cgtggtacat cgcgcggatg
     2821 ttacagccat agccggattc attgcccagc gaccagatga tgatcgacgg atggtttttc
     2881 tgcgcgtgaa tatggcgaac aatgcgctcg acgtagactt tttcccactg cggatcgtcg
     2941 gtaatacggc tgatatcgcc gacattagca aagccgtgcg attcgacgtc ggtttccgcc
     3001 atcacaaaca ggccgtagat atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga
     3061 gcggtacgca cggagttgat gttgtgctgc ttcatcaact ggagatcttt ctcgacgcga
     3121 tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc
     3181 atcacataac ggttattgat ccagaacaga ccgtcgcgca ctttgatatc acggaagcca
     3241 acgcgttgtg gtaccacttc cagaacgttg ccgttggcgt ctttcagcgt catgaccaga
     3301 tggtaaagat aaggggattc tgctgaccat tgctgcggct gttcgacagt aaaagcaaag
     3361 ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt
     3421 tcgccatcaa acagggtata ttccagcgtc gtgacgacag gggaggcggc gagattttcc
     3481 agcaccactt cgcaggaaag cgtggcatcg caataggctt cgtcaaagtc ggtacgcacg
     3541 gtgaaatcgt taatatgtgt tagttgtttt ccgatcagat aaacatcgcg gaagatcccc
     3601 gctgaccacc acatatcctg gtcttccacg taggtagagt ccgcccattg catcacgcgc
     3661 acacacaaca ggttgtcgcc ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg
     3721 cgactgccct tgctgaaacc cacatactga ccgttaacgt agacttcaaa ataggtttcg
     3781 acgccgtcaa atttaatcag cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata
     3841 cgttgatagg caccggttgg gttatcgctg ggaacaaacg gcacatcgat ggggaacgga
     3901 aaaccttcgt cggtatattg cagtttgccg tgaccttcca tttgccacat ggcggggacg
     3961 gtaatatgcc cccagtcagc cattaactca gaggtgaagg cttcaggcac ttgcagcggg
     4021 tggtcaaaaa agtggaaatt ccactgaccg cttaagggca gaaacaggct gctggtttcg
     4081 cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa aaaagtacgc acgtggcgca
     4141 agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat tcatagggcc tcccggtaga
     4201 gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa ttgctgggga gtttagtaaa
     4261 atttttacta aaaagtgaca ggtaaatgca tttactttaa ctggatcacg aaaataaacg
     4321 gcagcactgt
//
LOCUS       DAZOYL010000300         4298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZOYL010000300 DAZOYL010000000
VERSION     DAZOYL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.301"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            334..942
                     /locus_tag="ACHUOY_003574"
     CDS             334..942
                     /locus_tag="ACHUOY_003574"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR0684239.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1040..2431
                     /gene="selA"
                     /locus_tag="ACHUOY_003575"
     CDS             1040..2431
                     /gene="selA"
                     /locus_tag="ACHUOY_003575"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR0684240.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2428..4272
                     /gene="selB"
                     /locus_tag="ACHUOY_003576"
     CDS             2428..4272
                     /gene="selB"
                     /locus_tag="ACHUOY_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR0684241.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          966 a         1095 c         1257 g          980 t
ORIGIN      
        1 ggccacaccg gtgggggcac cgatgcgcac cccggctgaa ccctgaacaa tgctaccgcc
       61 atactgcgtc atgtcgccct gacgcgctgc cggttttccg ctcatctgct catccttgtt
      121 ttatcgggtt atttgtctgc ttccaggcaa aaccctacag atttataaga aagccttaag
      181 tgagatatcc atcagataga aacaattagg aaaatattta tatcaccatg ataaatatgt
      241 gatttatttg gaagtaacaa accatcaaca ttttctgccc tttttcccat caccaggcca
      301 tactgatatt tttcctcgca caaaggactc ggcatgaaac tcgtcggtag ctacaccagc
      361 ccgtttgtac gcaaactttc tattctgttg ttagaaaagg gcataacttt cgaatttatt
      421 aatgaactgc cctataacgc ggacaacggc gtggcgcaat ttaacccgtt aggaaaagtg
      481 ccggtgctgg tgaccgaaga gggcgaatgc tggtttgatt cgccgatcat cgctgaatat
      541 attgagttaa tgaatgtcgc tccggcgatg ttgccgcgcg atccgctgga gtcgttgcgg
      601 gtgcgcaaaa ttgaggcgct ggcggatggc attatggatg ccgggctggt atcggtgcgt
      661 gaacaggcgc gtccggcggc gcagcagtct gaagatgaat tgttacgcca gcgggagaaa
      721 atcaaccgca gtctggatgt gctggaagga tatctggtcg atggcacact caaaaccgat
      781 acggtcaatc tggcgactat cgccattgcc tgtgctgtcg gatatctcaa tttccgccgc
      841 gttgcgccag gttggtgtgt cgaccgccca cacttagtca aactggtcga aaacctgttt
      901 agccgcgaaa gttttgctcg caccgaaccg ccaaaggctt gatgcgcgat atgtcctcct
      961 gacccatctc acgttacaat ccgtggttat gttaaacgcc cttctccgtg tgagagggcc
     1021 ttgatcagcc aggtttccta tgacaaccga aacgcgttcc ctctatagtc aacttccggc
     1081 tattgatcgc ttattgcgcg atagctcctt cctttctttg cgtgatactt atggtcacac
     1141 ccgcgtggtg gaattgttgc gtcagatgct cgacgaagcg cgagaagtga ttcgtgacag
     1201 ccagacgctt cctgcgtggt gtgaaaactg ggcgcaagaa gtcgatgccc ggttgacgaa
     1261 agaggcgcag agcgcgctgc gtccggtgat caacctgacg ggaaccgtgc tgcataccaa
     1321 ccttggacga gctttacagg cggaagccgc ggtggaagcc gttgcgcagg ctatgcgttc
     1381 gccagtgacc ctcgagtacg atctggacga cgccggacgc ggacatcgcg atcgggcgct
     1441 ggcgcaactg ctgtgccgta ttacgggggc ggaagatgcc tgtatcgtca ataacaatgc
     1501 ggcggcggtg ttattgatgt tggcggccac tgccagcggg aaagaggtgg tagtatctcg
     1561 cggcgaattg gtggagattg gcggcgcgtt tcgtattccc gatgttatgc gtcaggcagg
     1621 ctgcacccta cacgaagtag ggaccaccaa ccgcacgcac gcgaatgatt atcgtcaggc
     1681 ggtgaatgaa aataccgcac tgttgatgaa agtacatacc agtaactaca gcattcaggg
     1741 gttcaccaaa gcgatagatg aagcggaact ggtggcgctc ggcaaagagc tggatgttcc
     1801 cgtagtgact gatttaggca gtggctcgct ggtcgatctt agccagtacg gtttgccgaa
     1861 agagccaatg ccgcaggagt tgattgcggc gggcgtcagt ctggtgagct tctccggcga
     1921 caagttgtta ggcgggccgc aggcagggat tatcgttggt aaaaaagaga tgatcgcccg
     1981 cctgcaaagc cacccgctga agcgtgcatt acgcgcggat aaaatgaccc tcgcggcgct
     2041 ggaagccacg ttgcgtcttt atttacaccc tgaagctctg agtgaaaaat taccgaccct
     2101 gcgcctgctt acccgcagcg cagaggtcat tcaaatccag gcacaacgtt tacaggctcc
     2161 ccttgccgca cattacggcg cggagtttgc ggtacaggtt atgccatgtc tttcgcagat
     2221 tggcagtggt tcgctgccgg ttgatcgcct gccgagcgcg gcattaacgt ttacacccca
     2281 tgatggacgc ggtagccacc ttgagtcatt agccgcccgc tggcgtgaat tgccagtgcc
     2341 gatgattggt cgtatttatg acggacgatt gtggctggat ttacgctgcc ttgaagatga
     2401 gcaacggttt ttggagatgt tgttgaaatg attattgcga ctgccggaca cgttgaccac
     2461 ggcaaaacaa ccttattgca ggtgattact ggcgtaaatg ctgaccgtct gccggaagaa
     2521 aaaaagcgcg gcatgaccat cgatctcggc tatgcctact ggccgcagcc ggatggtcgc
     2581 gtgcctagtt ttatcgacgt acccggtcat gaaaagtttc tttccaacat gctggcgggc
     2641 gttggtggta tcgatcacgc gctgttggtg gtggcgtgcg atgacggcgt gatggcgcag
     2701 acccgtgagc atctggcgat tttgcagctg accggtaacc cgatgctgac agtggcgctg
     2761 accaaagccg atcgcgtgga cgaagcgcgt gttgatgagg ttgaacgcca ggtaaaggag
     2821 gtgctgcggg aatacggttt tgctgaggca aaactgttta tcaccgcagc aaccgaaggt
     2881 cggggaattg atgccctgcg cgagcatctg cttcagttac caggacgtga acacgccagc
     2941 caacatagtg tccgcctcgc gatcgaccgc gcatttaccg taaaaggtgc cggtctggtc
     3001 gtcaccggta cggcgttaag cggggaagtg aaggtaggcg attcactctg gctgactggt
     3061 gtaaataaac cgatgcgtgt acgtgcgctg catgcacaaa accagccaac agaaaccgcc
     3121 catgccgggc agcgtatcgc gcttaacatc gcgggtgatg cggaaaaaga gcagattaac
     3181 cgtggcgact ggctgcttgc cgatgcgccg ccagagccgt tcacacgggt gattgtcgag
     3241 cttcaaaccc atacgccgct gacccagtgg cagccgctgc atattcacca cgccgccagc
     3301 cacgttacgg gacgcgtttc gctgctggaa gataaccttg ccgagctggt gttcgacacg
     3361 ccgctgtggc tggcggataa cgatcgctta gtgctgcgcg atatctcggc ccgcaacacg
     3421 ctggcggggg cgcgtgtggt gatgcttaac ccgccgcgtc gcggtaaacg taagccggaa
     3481 tatctgcaat ggctggcgtc tcttgcacgg gcgcagagcg atgccgatgc gttatctgtt
     3541 catctggaac gcggcgcggt taaccttgcg gatttcgcct gggcgcgcca gctcaacggc
     3601 gaagggatgc gcgaattgct gcaacagcct ggctatattc aggctggtta tagcttgttg
     3661 aatgcgccgg ttgccgcccg ctggcagcgg aaaattctcg acacattagc gacttatcat
     3721 gagcaacatc gcgatgaacc tggtcctggg cgcgaacgtc tgcgacgtat ggcgttgcca
     3781 atggaagatg aagcgctggt actgttgctg attgaaaaga tgcgcgaaag cggcgacatc
     3841 ctcagtcatc acggttggct gcatctgccg gatcacaaag cgggcttcag cgaagagcag
     3901 caggccatct ggcaaaaagc agagccgctg tttggtgacg agccgtggtg ggtgcgtgat
     3961 ctggcaaaag agacgggtac cgacgagcag gcaatgcgcc tgacgctacg ccaggcggcg
     4021 cagcaaggaa taattaccgc gatcgtcaaa gatcgttatt accgtaacga tcggattgtc
     4081 gaatttgcca atatgatccg cgatctcgat caggagtgtg gttcaacctg cgcggcggat
     4141 ttccgcgatc gcttaggcgt aggccgaaag ctggcaattc agattctgga atattttgac
     4201 cgcattggct ttacgcgtcg tcgtggaaat gatcatttat tgcgcgacgc attattattt
     4261 ccggaaaaat aaggaaatga ttaattaatt tttaaaat
//
LOCUS       DAZOYL010000301         4234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZOYL010000301 DAZOYL010000000
VERSION     DAZOYL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.302"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..331
                     /locus_tag="ACHUOY_003577"
     CDS             <1..331
                     /locus_tag="ACHUOY_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0684242.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHUOY_003578"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHUOY_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR0684243.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHUOY_003579"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHUOY_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR0684244.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHUOY_003580"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHUOY_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR0684245.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHUOY_003581"
     CDS             complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHUOY_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR0684246.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4035..>4234)
                     /locus_tag="ACHUOY_003582"
     CDS             complement(4035..>4234)
                     /locus_tag="ACHUOY_003582"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684247.1"
                     /translation="PFFAPSTAFCPIFAINNFPHKVISKPNSCSTFFLNIMSPAGNNL
                     IPVLGLKAVNDNHFHFVWVML"
BASE COUNT         1143 a         1041 c         1058 g          992 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaaca
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag gggc
//
LOCUS       DAZOYL010000302         4192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZOYL010000302 DAZOYL010000000
VERSION     DAZOYL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.303"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUOY_003583"
     CDS             complement(<1..215)
                     /locus_tag="ACHUOY_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0684248.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHUOY_003584"
     tRNA            complement(330..405)
                     /locus_tag="ACHUOY_003584"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHUOY_003585"
     tRNA            complement(412..486)
                     /locus_tag="ACHUOY_003585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHUOY_003586"
     tRNA            complement(603..687)
                     /locus_tag="ACHUOY_003586"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHUOY_003587"
     tRNA            complement(696..771)
                     /locus_tag="ACHUOY_003587"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUOY_003588"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUOY_003588"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR0684249.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUOY_003589"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUOY_003589"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR0684250.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUOY_003590"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUOY_003590"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR0684251.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1040 c          936 g         1136 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatct tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 acttcccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAZOYL010000303         4186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZOYL010000303 DAZOYL010000000
VERSION     DAZOYL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.304"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..325
                     /locus_tag="ACHUOY_003591"
     CDS             <1..325
                     /locus_tag="ACHUOY_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0684252.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            552..1817
                     /locus_tag="ACHUOY_003592"
     CDS             552..1817
                     /locus_tag="ACHUOY_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0684253.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1829..2695
                     /gene="ydiB"
                     /locus_tag="ACHUOY_003593"
     CDS             1829..2695
                     /gene="ydiB"
                     /locus_tag="ACHUOY_003593"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR0684254.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2726..3484
                     /gene="aroD"
                     /locus_tag="ACHUOY_003594"
     CDS             2726..3484
                     /gene="aroD"
                     /locus_tag="ACHUOY_003594"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR0684255.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3628..>4186
                     /locus_tag="ACHUOY_003595"
     CDS             3628..>4186
                     /locus_tag="ACHUOY_003595"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR0684256.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFI"
BASE COUNT         1093 a          938 c         1004 g         1151 t
ORIGIN      
        1 attttatgtg gtgataatat ttgcttttgt cattggtttt acctctgctg gaggtgttgt
       61 gcaaattggc ctgacgttaa tggctgaacg tttcccttac gctaaaggta aagctacagg
      121 gatctattac agtgcgggca gtattgcgac ctttactatt ccgttgatta cggctcatct
      181 gtcccaaaga agtattgccg atattatgtg gttcgatacc gccatcgctg ccatcggttt
      241 tttactggca ctgtttatcg gcttacgcag ccgcaaaaaa acgcggcatc actcgctaaa
      301 ggaaaatgtc gctccgggtg ggtaatgcaa tattcttttc aggtcatgca agatcttacg
      361 gataaataac tctttctgcg ctaactaagg aaaatcgcga tcaaaaacaa actatgacat
      421 gcaatattcc tggaaacata aactttatgc catgtaccca gggaaaatca tcttcagtat
      481 agtaattatg taaaccgtcg gagaacaata cgtacggtaa cgaaattatc tttcagcaag
      541 gagctgtgaa aatgtctcaa aataaggctt tcagcacgcc atttatcctg gctgttcttt
      601 gtatttactt cagctatttc ctgcacggca ttagtgttat tacgcttgcc caaaatatga
      661 catctctggc agaaaagttt tccactgaca acgcgggcat tgcctattta atttccggta
      721 tcggtttggg gcgattgatc agtattttat tcttcggtgt gatctccgat aagtttggtc
      781 gtcgggcggt gatattaatg gcagtaataa tgtatctgct attcttcttt ggtattcccg
      841 cttgcccgaa tttaactctc gcctacggtc tggcagtgtg cgtaggtatc gctaactcag
      901 cgctggatac gggtggctac cccgcgctca tggaatgctt tccgaaagcc tctggttcgg
      961 cggtcatact ggttaaagcg atggtgtcat ttgggcaaat gttctaccca atgctggtga
     1021 gctatatgct gctcaataat atctggtacg gctatgggct gattattccg ggtattctgt
     1081 ttgtactgat cacgctgatg ctgttgaaaa gcaaattccc cagccagttg gtggacgcca
     1141 gcgtagctaa tgaattaccg caaatgaaca gcaaaccgtt agtctggctg gaaggtgttt
     1201 catcggtact gttcggtgta gccgcattct cgacctttta tgtgattgtg gtgtggatgc
     1261 ccaaatatgc gatggctttt gctggtatgt cagaagctga ggcattaaaa accatctctt
     1321 attacagtat gggctcgttg gtctgtgtct ttatttttgc cgcactactg aaaaaaatgg
     1381 tccggcccat ctgggctaat gtatttaact ctgcgctggc aacaataaca gctgccatta
     1441 tctacctgta cccttctcca ctggtgtgta atgccggcgc ctttgttatc ggtttctcag
     1501 cagctggcgg cattttacag ctcggtgttt cggtcatgtc agagtttttc cccaaaagca
     1561 aagccaaagt caccagtatt tatatgatga tgggtggact ggctaacttt gttattccgc
     1621 tgattaccgg ttatctgtcg aacatcggcc tgcaatatat cattgttctc gattttactt
     1681 tcgcgctgct ggccctgatt accgcaatta ttgtttttag ccgctattac cgcgttttta
     1741 ttattcctga gaatgatgtg cggtttggcg aacgcaaatt tagcacccgg ttaaacacaa
     1801 ttaagcatag aggttaaagg agttaattat ggatgttacc gcaaaatacg aattgattgg
     1861 gttgatggct tatcctatcc gccacagttt atcgcctgaa atgcagaata aagccttaga
     1921 aaaagcggga ttgccattta cctatatggc cttcgaagtg gataacgata gctttcccgg
     1981 agcaattgaa ggattaaaag ccctcaaaat gcgcggaact ggtgtgtcga tgccgaacaa
     2041 acaactggcg tgtgaatatg ttgatgaatt aacgccagca gccaaactgg tgggtgccat
     2101 caacaccatc gttaatgatg atggctatct gcgtggctat aacaccgacg gcacgggcca
     2161 tattcgcgcc attaaagaga gcggttttga tatcaaaggc aaaacgatgg tgctgttagg
     2221 ggccggaggt gcctcaacag cgattggcgc gcagggggca attgaaggtt taaaagaaat
     2281 taaactcttt aaccgtcggg atgagttctt cgataaagcc ctcgccttcg cgcagcgggt
     2341 taacgaaaac accgattgtg tcgtcacggt taccgatctc gccgatcagc aagcctttgc
     2401 tgaagtcctg gcttccgccg acattttaac caatggcaca aaagtaggta tgaaccccct
     2461 tgagaatgaa tcattggtta atgatatcag tctgttacat ccgggactgc tggtcactga
     2521 atgcgtgtat aacccgcata tgacgaagtt attgcagcag gcacaacaag caggttgcaa
     2581 aacgattgat ggatacggca tgttgttgtg gcaaggggct gaacagttca cgttatggac
     2641 tggcaaagat ttccctctgg aatatgttaa acaggtcatg gggttcggtg cctgacaggc
     2701 tgaccgcgtg cagaaagggt aaaaaatgaa aaccgtaact gtaaaagatc tcgtcattgg
     2761 tgcgggcgca cctaaaatca tcgtctcgct gatggcgaaa gatatcgccc gcgtgaaatc
     2821 cgaagctctc gcctatcgtg aagcggactt tgatattctg gaatggcgtg tggaccactt
     2881 tgccgacctc tccaatgtgg agtctgtcat ggcggcggca aaaattctcc gtgaaaccat
     2941 gccagaaaaa ccgctgctgt ttaccttccg cagtgccaaa gaaggcggcg agcaggcgat
     3001 ttccaccgag gcttatattg ctctcaatcg tgcagccatc gacagcggcc tggttgatat
     3061 gatcgatctg gagttattta ccggcgatga tcaggttaaa gaaaccgtcg cctacgccca
     3121 cgcgcatgat gtgaaagttg tcatgtccaa ccatgacttc cataaaacgc cggaagccga
     3181 agaaatcatt gcccgtctgc gcaaaatgca gtccttcgac gccgatattc ctaagattgc
     3241 gctgatgccg caaagtacca gcgatgtgct gacgttgctt accgcgaccc tggagatgca
     3301 ggagcagtat gccgatcgtc caatcatcac gatgtcgatg gcaaaaactg gcgtaatttc
     3361 tcgtctggct ggtgaagtat ttggctcggc ggcaactttt ggtgcggtaa aaaaagcctc
     3421 tgcaccaggg caaatctcgg taaatgattt acgcacggta ttaactattt tacaccaggc
     3481 ataagcaata atatttcggc gggaacaccc tccccgccga acttaaaaaa tatatttaac
     3541 cgtatttaat aaaaatattt cgtgagtctc tgtgcgctaa ttctccatct ggcgtaggga
     3601 aaatcacatc tgaatcagga attaacaatg aaacctgtaa aaccacctcg tattaatgga
     3661 cgagtgccgg tcctgtcggc acaggaagcg gtgaattata ttcccgacga agcaacactt
     3721 tgtgtgttag gcgctggcgg cggtattctg gaagccacca cgttaattac agctcttgct
     3781 gataaatata aacagactca aacgccacgt aatttatcga ttattagtcc gacagggctt
     3841 ggcgatcgcg ccgaccgtgg tattagtcct ctggcgcaag aaggtctggt gaaatgggca
     3901 ttatgtggtc actggggaca atcgccgcgt atttctgaac tcgcagaaca aaataaaatt
     3961 attgcttata actatccaca aggtgtactt acacaaacct tacgcgccgc cgcagcccac
     4021 cagcctggta ttattagtga tattggcatc gggacatttg tcgatccacg ccagcaaggc
     4081 ggcaaactga atgaagtcac gaaagaagac ctgattaaac tggtcgagtt tgataacaaa
     4141 gaatatctct attacaaagc gattgcgcca gatatcgcct tcattc
//
LOCUS       DAZOYL010000304         4130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZOYL010000304 DAZOYL010000000
VERSION     DAZOYL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.305"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            115..528
                     /locus_tag="ACHUOY_003596"
     CDS             115..528
                     /locus_tag="ACHUOY_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR0684257.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="ACHUOY_003597"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="ACHUOY_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR0684258.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="ACHUOY_003598"
                     /pseudo
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="ACHUOY_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="ACHUOY_003599"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="ACHUOY_003599"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR0684259.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="ACHUOY_003600"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="ACHUOY_003600"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR0684260.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="ACHUOY_003601"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="ACHUOY_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR0684261.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3794..3881)
                     /locus_tag="ACHUOY_003602"
     tRNA            complement(3794..3881)
                     /locus_tag="ACHUOY_003602"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3845..3847),aa:Ser,seq:tga)
BASE COUNT         1100 a         1047 c         1017 g          966 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     2461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
     3781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     3841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     3901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     3961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     4021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     4081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa
//
LOCUS       DAZOYL010000305         4118 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZOYL010000305 DAZOYL010000000
VERSION     DAZOYL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.306"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..83
                     /locus_tag="ACHUOY_003603"
     CDS             <1..83
                     /locus_tag="ACHUOY_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684262.1"
                     /translation="DVMLEQQNEDGSIELLERCPPRMGNL"
     gene            complement(135..854)
                     /gene="fadR"
                     /locus_tag="ACHUOY_003604"
     CDS             complement(135..854)
                     /gene="fadR"
                     /locus_tag="ACHUOY_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR0684263.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            1075..2616
                     /gene="nhaB"
                     /locus_tag="ACHUOY_003605"
     CDS             1075..2616
                     /gene="nhaB"
                     /locus_tag="ACHUOY_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR0684264.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            2762..3292
                     /gene="dsbB"
                     /locus_tag="ACHUOY_003606"
     CDS             2762..3292
                     /gene="dsbB"
                     /locus_tag="ACHUOY_003606"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR0684265.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(3338..>4118)
                     /gene="umuC"
                     /locus_tag="ACHUOY_003607"
     CDS             complement(3338..>4118)
                     /gene="umuC"
                     /locus_tag="ACHUOY_003607"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0684266.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
BASE COUNT          892 a         1059 c         1042 g         1125 t
ORIGIN      
        1 ttgatgtgat gctcgaacag caaaacgaag acggcagcat cgagttgctg gaacgttgcc
       61 cgccaagaat gggaaatctg taaaaacaac aaaaaacccc ccgtttgagg ggtttgctct
      121 ttaaacggaa gggattatcg cccctgaatg gctaaatcac ccggcagatt tttctgcatc
      181 cggtgccaaa tctcgccact ctcatgccca tagcgacgca ctgtttcgta cacctgatcg
      241 tgcgcgcctt cactgcacaa cgccgacagt ttgtggtaga agcccagcgc cagactgcgc
      301 gcttccggat tggcgaaata gtgacgacca atgcgcgtat acagcccttt catcccgtta
      361 aggatcagac cgtaaatcgg gttgccggaa gcaaacgcca ggccgcggaa tatgttgtaa
      421 tccagctcgg caaaggcatc ggcgtgatcg gccacttcat tagcggtagc cagcacttcc
      481 tgcgctttat cgggatgctg acgaaacgcg gtgcgaataa aaatagtgga aatattggta
      541 cgcaccgaca gcaaattatc aataagctgc ggcacacttt cgtgatccag tcgcgccagt
      601 gtttcaagga tatttaaacc ggaagtttcc cagaaattat tcaccttcgt cggcttgcca
      661 tgttgaatgg tcaaccagcc atctcgtgcc agacgctgta acacttcacg taacgtagta
      721 cgcgttacgc caattaattc tgaaagttca cgttctgcgg gcaaaatagt cccgggaggg
      781 aagcggttat tccagatact ttcaataatg tactcttccg cgaaacccgc cgggctttgc
      841 gccttaatga ccatagtgag atttccataa cacagcaaaa caaagttgga ctcatcatac
      901 cagagggcta actacgctga tagcagacaa aaagaagaaa aagggatcaa ggcttcattt
      961 tccgcatctc tttccccttg actatcaaga cttgcctcac tcctgcctga ccgttaagct
     1021 ttgcgtttaa acataaaaac atcattgatt atttgtaagg taagggaaac catcatggag
     1081 atctcctggg gccgcgcgct atggcgcaat tttttgggcc agtcccccga ctggtacaaa
     1141 ctcgccctca tcattttctt aatcgtaaac ccgttaattt tcctcatcag ccctttcgtc
     1201 gcgggctggt tgctggtagc ggaatttatt ttcactctgg cgatggccct gaaatgctac
     1261 ccgctgctcc ccggtggtct gttggctatc gaagcggtat tcatcggcat gaccagcgcg
     1321 gaacacgtcc gtgaagaggt ggcggcaaat cttgaagtct tgctgttact gatgtttatg
     1381 gtggcgggta tctattttat gaaacagcta ttgctgttca tatttacccg tttgctgtta
     1441 agcattcgct ccaaaatgct gctgtcgctc tctttttgcg tggcggcggc gttcctctcc
     1501 gcgttcctcg atgccttaac cgtcgtggcg gtggtgatca gcgttgcagt cggtttttat
     1561 ggtatttatc accgcgtagc ctcttcccgt actgaagata ccgacctgca agacgatagt
     1621 catatcgaca agcattacaa agtggttctg gaacagttcc gtggctttct gcgtagcctg
     1681 atgatgcatg ccggtgtcgg caccgcatta ggcggcgtga tgaccatggt gggcgaacca
     1741 cagaacctga tcatcgctaa agcggctggc tggcattttg gcgatttctt cctgcgcatg
     1801 tcgccggtga ccgttccggt tctgatttgt ggcctgttaa cctgcctgct ggtggagaag
     1861 ctgcgttggt ttggctacgg tgaaacgctg ccggagaaag tccgcgaagt gctgcagcag
     1921 tttgacgatc aaagccgcca ccagcgtacc cgtcaggata aaatccgtct gattgtccag
     1981 gcgattattg gcgtctggtt ggtgactgcg ctggcgttgc atctggcgga agttggtttg
     2041 attggtttgt cagtcattat tctggcgaca tcattgatcg gtgtcaccga tgagcatgct
     2101 atcggtaaag ccttcaccga atctctgcca ttcaccgcac tgttgacggt gtttttctcg
     2161 gtagtcgcgg tgattatcga ccaacaactg ttttcgccga ttattcagtt tgtgttgcag
     2221 gcatcggaac atgctcagct gtcgctgttc tatatattca acggtctgct gtcatccatt
     2281 tcggataacg tcttcgtggg gacgatttat atcaacgaag cgaaagcggc aatggaaagt
     2341 ggcgctatca cgttgaagca atacgagctg ctggcggtcg ccattaatac cggtaccaat
     2401 ctgccctctg tcgctacgcc gaacggtcag gctgcgttcc tattcctgct gacctctgca
     2461 ctcgcgccat tgattcgcct ctcttatggc cgcatggtgt ggatggccct gccttacacc
     2521 ctcgtcctga cactcgtcgg actgctctgc gtcgagttta cgcttgcccc tgtaaccgaa
     2581 tggtttatgc aaatgggctg gatagcaacg ctttgataac aacttaccgg gcatataaat
     2641 gcccggtttg ccttttcgcc cgataattgt ccaattgcgt cctatttttg ctgcctcctg
     2701 gtggcgcagc gaatgaattg gtttaaactg cgcactctat gcatattgca gggaaatgat
     2761 tatgttgcga tttttgaacc aatgttcaca aggccggggc gcgtggctgt tgatggcgtt
     2821 tactgctctg gcactggaac tgacggcgct gtggttccag catgtgatgt tactgaaacc
     2881 ttgcgtgctc tgtatttatg aacgctgcgc gttattcggc gttctgggtg ctgcgctgat
     2941 tggcgcgatc gccccgaaaa ctccgctgcg ttatgtagcg atggttatct ggttgtatag
     3001 tgcgttccgc ggtgtgcagt taacttacga gcacaccatg cttcagctct atccttcgcc
     3061 gtttgccacc tgtgatttta tggttcgttt cccggaatgg ctgccgctgg ataaatgggt
     3121 gccgcaagtg tttgtcgcct ctggcgattg cgccgagcgt cagtgggatt ttttaggtct
     3181 ggaaatgccg cagtggctgc tcggtatttt tatcgcttac ctgattgtcg cggtgctggt
     3241 gatgatttcc cagccgttta aagcgaaaaa acgtgatctg ttcggtcgct aatccattcg
     3301 gcgctcctgc gggagcgctt ttttcctgcc gctatattta tttgacccgc agtaaatcag
     3361 aacttcgcgt tgtataacgt ggtgaaagca tggctcgctt catctgccat tgttgttgga
     3421 tcccctgccc ggcaaaatag agtgttcctc tgccctcttt agcattcagt gtatccatta
     3481 ccgtcatcaa ttgctcactc ccggggcgcg gtgcgttgtc atcgaataaa ttgagctgcg
     3541 cgactccctg actgaagaaa tcccccagca tcacgcccgc tttttggtag cgatggcccg
     3601 cttgccagat ggcatccaga gatcgcgtag cggcgttaat gatatccctg ctgtcctgag
     3661 tgggcgtcag cagttttacc gacgcgctat tgccgtaata aggttcattg agcgcaaatg
     3721 gtgacgtctt aataaatgtg gagataaacc gacaatattg atgctcactg cgaagttttt
     3781 ccgccgcccg ggcagcgtaa ctacaaatgg cctgccgcat cgacgtataa tccgtgatac
     3841 gttcaccaaa cgagcgggaa cagataattt cctgctttgt cggtgcaaat tcttccagtt
     3901 gcaaacaggg ttcaccgcgc agttcacgca ccgttctttc gagcacgaca ttaaaatgtt
     3961 tacggataaa ccggatatct gtatccgcca aatcaagaac ggttttgatc cccatcgcgt
     4021 ccagtttttt gctgatccgc cgtccaatcc cccagacgtc atccacgggg agagcagaca
     4081 ttaatttacg ctggcgttcc agatttgata aatccacc
//
LOCUS       DAZOYL010000306         4063 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZOYL010000306 DAZOYL010000000
VERSION     DAZOYL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.307"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            58..1572
                     /gene="caiT"
                     /locus_tag="ACHUOY_003608"
     CDS             58..1572
                     /gene="caiT"
                     /locus_tag="ACHUOY_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR0684267.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1603..2745
                     /gene="caiA"
                     /locus_tag="ACHUOY_003609"
     CDS             1603..2745
                     /gene="caiA"
                     /locus_tag="ACHUOY_003609"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR0684268.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2874..4063
                     /gene="caiB"
                     /locus_tag="ACHUOY_003610"
                     /pseudo
     CDS             2874..4063
                     /gene="caiB"
                     /locus_tag="ACHUOY_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT          956 a         1035 c         1087 g          985 t
ORIGIN      
        1 aaaataaaac aaacgggaac gcaaaataaa tattcgtttt cacagtggaa ttaactcatg
       61 aagaatgaaa agagaaaaac gggaatagaa ccgaaggttt tctttccgcc gttaataatc
      121 gtcggcatac tttgttggct tacagtcaga gatctggatg cagcgaatgt cgttattaat
      181 gctgtattca gttacgtcac caatgtatgg ggatgggcat ttgaatggta tatggtggtg
      241 atgcttttcg gttggttctg gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac
      301 gaaccacctg aatttagtac cgccagttgg atctttatga tgttcgcctc ctgtacgtct
      361 gctgccgtac tgttctgggg atcgattgag atctactact acatctccac cccgccgttt
      421 ggcttagaac cgaactcgac aggggcgaaa gagttggggc tggcttacag cttgttccac
      481 tggggacctc tgccgtgggc cacttacagc ttcctttcag tcgccttcgc ttacttcttc
      541 tttgtccgca aaatggaagt gattcgcccc agctcgacac tggtgccgct ggtaggtgaa
      601 aaacacgcca aagggttgtt cggcactatc gtcgacaact tctatctcgt cgccttgatc
      661 ttcgcgatgg gtaccagtct gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg
      721 ttgtttggca ttccgcatac cctgcaactg gacgctatca tcattacctg ctggattatc
      781 ctcaacgcca tttgcgtcgc ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg
      841 cgtagttacc tgagcttcct gatgctgggt tgggtgttca ttgtcagcgg tgccagcttc
      901 atcatgaact acttcaccga ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg
      961 ttctataccg atcccatcgc taaaggcggc ttcccgcagg gctggaccgt gttctactgg
     1021 gcatggtggg tgatttatgc tatccagatg agtatcttcc tcgcccgcat ctcccgtggt
     1081 cgtactgtgc gtgaactgtg cttcggcatg gtgctggggc tgacagcgtc aacctggatc
     1141 ctgtggactg tactcggtag taacactctg ctgttgatag ataaaaacat catcaacatt
     1201 ccaaatctga tcgaacagta cggtgtggcg cgcgccatca ttgaaacctg ggccgctctg
     1261 ccactcagca ccgccaccat gtggggcttc ttcatcctct gctttattgc caccgttaca
     1321 ctggttaacg cctgctctta taccctggcg atgtccactt gccgcgaagt acgcgatggt
     1381 gaagaaccac ctctgctggt gcgtatcggt tggtcaattc tggttggcat tatcggtatt
     1441 gttctgctgg cgctcggcgg cctgaaaccg attcaaaccg ccattatcgc cggaggatgc
     1501 ccgctgttct tcgtcaacat tatggtgacg ctctccttta ttaaagacgc gaaacagaac
     1561 tggaaagatt aattaacccc caaaatatca agaggttgaa agatggattt taatttaaat
     1621 gatgagcagg aactgtttgt cgccggtatc cgcgaactga tggccagcga aaactgggag
     1681 gcctattttg ccgagtgcga ccgtgacagc gtctacccgg aacgttttgt caaagcactg
     1741 gcggatatgg gtatcgacag tctgctgatc cctgaagagc acggtggtct ggacgcgggg
     1801 tttgttactc tcgccgccgt gtggatggag ctgggacgtc tgggggcacc aacctatgtg
     1861 ctgtaccagt tgccgggcgg gttcaacacc ttcctgcgcg aaggcacaca agagcagatc
     1921 gacaaaatta tggctttccg cggcaccggt aagcagatgt ggaactcagc gattaccgaa
     1981 ccgggcgcgg gctccgacgt gggtagcctg aaaacgactt atacccgtag aaatggtaag
     2041 atttatctta atggtagtaa gtgttttatt accagcagcg cctacacccc gtacatcgtg
     2101 gtgatggcgc gcgacggggc ttctccggac aaacctgtct acaccgaatg gtttgttgat
     2161 atgagcaaac cgggcatcaa agtgaccaaa cttgaaaagc tcggtctgcg tatggatagc
     2221 tgctgtgaaa tcacctttga cgacgtggaa ctggacgaga aagacatgtt cggtcgggaa
     2281 ggtaacggct ttaaccgcgt caaagaagag ttcgaccatg aacgtttcct ggtagccctc
     2341 accaactacg gtacggcgat gtgcgctttt gaagatgcgg cgcgctacgc caatcagcgc
     2401 gtgcagtttg gcgaggctat tggtcgtttc cagttgattc aggaaaaatt cgcccacatg
     2461 gcgatcaaat taaactccat gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac
     2521 ggcaccatca cctctggcga tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt
     2581 gaagttgtgg atagcgcaat gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc
     2641 atcagccgct tctggcgtga tctgcgtgta gaccgcgtct ccgggggatc tgacgaaatg
     2701 cagatcctga cgctgggtcg cgcggtgctg aagcaatacc gctaagtccg tgcgctgcct
     2761 gatgcgacgc taatgcgtct tatcaggcct acgggagcac gaatgtaggc cggataaagc
     2821 gtttacgccg catccggcag tcatgcacta cataaatttt tcaggagcta ataatggatc
     2881 atctacccat gccgaaattc gggccgttgg ccggattgcg cgttgtcttc tccggtatcg
     2941 aaatcgccgg gccgttcgcc gggcaaatgt tcgcagaatg gggtgcggaa gttatctgga
     3001 tcgagaacgt cgcctaggcc gacaccattc gcgttcaacc gaactacccg cagctctccc
     3061 gccgcaattt gcacgcgctg tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc
     3121 tgaaattaat ggaaaccacc gatatcttca tcgaagccag caaaggtccg gcctttgccc
     3181 gtcgtggcat taccgatgaa gtactgtggc agcacaaccc gaaactggtt atcgctcacc
     3241 tgtccggttt tggtcagtac ggcaccgagg agtacaccaa tcttccggcc tataacacca
     3301 tcgcccaggc cttcagtggt tacctgattc agaacggtga tgttgaccag ccaatgcctg
     3361 ccttcccgta taccgccgat tacttttctg gcctgaccgc caccacggcg gcgctggcag
     3421 cactgcataa agtgcgtgaa accggtaaag gcgaaagtat cgacatcgcc atgtatgaag
     3481 tgatgctgcg tatgggccag tacttcatga tggattactt caacggcggc gaaatgtgcc
     3541 cgcgcatgag caaaggtaaa gatccctact acgccggttg cggtctgtat aaatgcgccg
     3601 acggctacat cgtgatggaa ctggtgggca ttacccaaat tgaagagtgc tttaaagata
     3661 ttgacctcgc acatctactc ggtacgccgg aaatcccgga aggcactcag cttatccacc
     3721 gtatcgaatg cccttacggc ccactggtag aagaaaaact cgatgcctgg ctggcggcac
     3781 ataccatcgc agaagttaaa gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac
     3841 tgaccgtacc ggaactggaa agcaatccac agtatgttgc ccgtgaatca atcactcagt
     3901 ggcaaacgat ggatggtcgc acctgcaaag ggccgaacat catgccgaaa ttcaaaaata
     3961 accccggaca aatctggcgc ggaatgccct cacatggcat ggacacggct gccattttga
     4021 aaaatatcgg ctacagcgaa aacgacattc aggagttggt cag
//
LOCUS       DAZOYL010000307         4061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZOYL010000307 DAZOYL010000000
VERSION     DAZOYL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.308"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(225..872)
                     /gene="ychE"
                     /locus_tag="ACHUOY_003611"
     CDS             complement(225..872)
                     /gene="ychE"
                     /locus_tag="ACHUOY_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR0684269.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1350..4025
                     /gene="adhE"
                     /locus_tag="ACHUOY_003612"
                     /gene_synonym="adhC"
     CDS             1350..4025
                     /gene="adhE"
                     /locus_tag="ACHUOY_003612"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR0684270.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1129 a          998 c          941 g          993 t
ORIGIN      
        1 aattactaat atcatttcaa ttattcttca gcaagcccag gattatgatg tggcgaaaat
       61 aacatgatgg tgaggagaag cagttaagga aatccttata actaactgta atttgagaag
      121 aatattttta cctattcctt ttgcttttga taactgcgcg ggaacaaagt aaaaataatg
      181 acgaagtaaa cggagcatgg cagctccgtt tcatttgaaa ggaattaatt aagcaggccg
      241 gggaaaatcc ccttaatacc agtaacgata aattcaatcc ccaatgccat cagcaatagc
      301 cccataatac gcgtaatcac gttgatgccg gtctggcgta aaacccgtac cagccacggt
      361 gccatgcgga acaatcccca gcaacataaa gcgaacaatg caatagccac aaagaaacca
      421 aacagatagc taatgctgtg ataacgcgta ccccagacga tggtagaact gatcgcccct
      481 ggccccgcca tcaacggcaa cgccaatggc accacaccaa tgctttcacg taccgcggtt
      541 tctgattttt cttgcttgtt ctgtttatcc tcgccaagct tgccgctgat catcgacatc
      601 gctatcgtca ccaccaggat gccccctgcg atacggaacg aatcaattga tataccaaaa
      661 agttgcagaa tcgtgtcgcc gagaaaaagc gagatccaca agataatggc cacagacagg
      721 ttggctgtaa ggttagtttt gtttcgcgct gccgctgtct gataactggt catgctgata
      781 aagacgggaa taatccctac cgggttgacc agcgcaaata acccgatgaa aaatttgaag
      841 taaacgggaa aatcaaaaaa ggtctgaatc acggttagct ccgaagcaaa agccggataa
      901 tgttagccat aaattaaggt tgaaaagacg cgctgacaat acgccttttg acagcatttt
      961 tcacctccta actacttaaa attgctatca ttcgttattg ttatctagtt gtgcaaaaca
     1021 tgctaatgta gccaccaaat catactacaa tttattaact gttagctata atggcgaaaa
     1081 gcgatgctga aaggtgtcag cttagcaaaa atttgatttg gatcacgtaa tcagtaccca
     1141 gaagtgagta atcttgctta cgccacctag aagtgacgca ttagagataa taactctaat
     1201 gtttaaactc ttttagtaaa tcacagtgag tgtgagcgcg agtaagcttt tgattttcat
     1261 aggttaagca aatcatcacc gcactgacta tactctcgta ttcgagcaga tgatttacta
     1321 aaaaagttta acattatcag gagagcatta tggctgttac taatgtcgct gaacttaacg
     1381 cactcgtaga gcgtgtaaaa aaagcccagc gtgaatatgc cagtttcact caagagcaag
     1441 tagacaaaat cttccgcgcc gccgctctgg ctgctgcaga tgctcgaatc ccactcgcga
     1501 aaatggccgt tgccgaatcc ggcatgggta tcgtcgaaga taaagtgatc aaaaaccact
     1561 ttgcttctga atatatctac aacgcctata aagatgaaaa aacctgtggt gttctgtctg
     1621 aagacgacac ttttggtacc atcactatcg ctgaaccaat cggtattatt tgcggtatcg
     1681 ttccgaccac taacccgact tcaactgcta tcttcaaatc gctgatcagt ctgaagaccc
     1741 gtaacgccat tatcttctcc ccgcacccgc gtgcaaaaga tgccaccaac aaagcggctg
     1801 atatcgttct gcaggctgct atcgctgccg gtgctccgaa agatctgatc ggctggatcg
     1861 atcaaccttc tgttgaactg tctaacgcac tgatgcacca cccagacatc aacctgatcc
     1921 tcgcgactgg tggtccgggc atggttaaag ccgcatacag ctccggtaaa ccagctatcg
     1981 gtgtaggcgc gggcaacact ccagttgtta tcgatgaaac tgctgatatc aaacgtgcag
     2041 ttgcatctgt actgatgtcc aaaaccttcg acaacggcgt aatctgtgct tctgaacagt
     2101 ctgttgttgt tgttgactct gtttatgacg ctgtacgtga acgttttgca acccacggcg
     2161 gctatctgtt gcagggtaaa gagctgaaag ctgttcagga tgttatcctg aaaaacggtg
     2221 cgctgaacgc ggctatcgtt ggtcagccag cctataaaat tgctgaactg gcaggcttct
     2281 ctgtaccaga aaacaccaag attctgatcg gtgaagtgac cgttgttgat gaaagcgaac
     2341 cgttcgcaca tgaaaaactg tccccgactc tggcaatgta ccgcgctaaa gatttcgaag
     2401 acgcggtaga aaaagcagag aaactggttg ctatgggcgg tatcggtcat acctcttgcc
     2461 tgtacactga ccaggataac caaccggctc gcgtttctta cttcggtcag aaaatgaaaa
     2521 cggctcgtat cctgattaac accccagcgt ctcagggtgg tatcggtgac ctgtataact
     2581 tcaaactcgc accttccctg actctgggtt gtggttcctg ggggggtaac tccatctctg
     2641 aaaacgttgg tccgaaacac ctgatcaaca agaaaaccgt tgctaagcga gctgaaaaca
     2701 tgttgtggca caaacttccg aaatctatct acttccgccg tggctccctg ccaatcgcgc
     2761 tggatgaagt gattactgat ggccacaaac gtgcgctcat cgtgactgac cgcttcctgt
     2821 tcaacaatgg ttatgctgat cagatcactt ccgtactgaa agcagcaggc gttgaaactg
     2881 aagtcttctt cgaagtagaa gcggacccga ccctgagcat cgttcgtaaa ggtgcagaac
     2941 tggcaaactc cttcaaacca gacgtgatta tcgcgctggg tggtggttcc ccgatggacg
     3001 ccgcgaagat tatgtgggtt atgtacgaac atccggaaac tcacttcgaa gagctggcgc
     3061 tgcgctttat ggatatccgt aaacgtatct acaagttccc gaaaatgggc gtgaaagcga
     3121 aaatgatcgc tgtcaccacc acttctggta caggttctga agtcactccg tttgcggttg
     3181 taactgacga cgctactggt cagaaatatc cgctggcaga ctatgcgctg actccggata
     3241 tggcgattgt cgacgccaac ctggttatgg acatgccgaa gtccctgtgt gctttcggtg
     3301 gtctggacgc agtaactcac gccatggaag cttatgtttc tgtactggca tctgagttct
     3361 ctgatggtca ggctctgcag gcactgaaac tgctgaaaga atatctgcca gcgtcctacc
     3421 acgaagggtc taaaaatccg gtagcgcgtg aacgtgttca cagtgcagca actatcgcgg
     3481 gtatcgcatt tgcgaacgcc ttcctgggtg tatgtcactc aatggcgcac aaactgggtt
     3541 cccagttcca tattccgcac ggtctggcaa acgccctgct gatttgtaac gttattcgct
     3601 acaatgcgaa cgacaacccg accaagcaga ctgcattcag ccagtatgac cgtccgcagg
     3661 ctcgccgtcg ttatgctgaa attgccgacc acttgggtct gagcgcaccg ggcgaccgta
     3721 ctgctgctaa gatcgagaaa ctgctggcat ggctggaaac gctgaaagct gaactgggta
     3781 ttccgaaatc tatccgtgaa gctggcgttc aggaagcaga cttcctggcg aacgtggata
     3841 aactgtctga agatgcattc gatgaccagt gcaccggcgc taacccgcgt tacccgctga
     3901 tctccgagct gaaacagatc ctgctggata cctactacgg tcgtgattat gtagaaggtg
     3961 aaactgcagc gaaaaaagaa gccgctccgg ctaaagctga gaaaaaagcg aaaaaatccg
     4021 cttaatcagt agcgctgtct ggcaatataa acggcccctt c
//
LOCUS       DAZOYL010000308         4024 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZOYL010000308 DAZOYL010000000
VERSION     DAZOYL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.309"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..256
                     /locus_tag="ACHUOY_003613"
     CDS             <1..256
                     /locus_tag="ACHUOY_003613"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0684271.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            315..1772
                     /gene="dtpC"
                     /locus_tag="ACHUOY_003614"
     CDS             315..1772
                     /gene="dtpC"
                     /locus_tag="ACHUOY_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR0684272.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            2009..3526
                     /gene="lysS"
                     /locus_tag="ACHUOY_003615"
     CDS             2009..3526
                     /gene="lysS"
                     /locus_tag="ACHUOY_003615"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0684273.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(3645..3818)
                     /gene="ghoT"
                     /locus_tag="ACHUOY_003616"
     CDS             complement(3645..3818)
                     /gene="ghoT"
                     /locus_tag="ACHUOY_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR0684274.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(3846..>4024)
                     /gene="ghoS"
                     /locus_tag="ACHUOY_003617"
     CDS             complement(3846..>4024)
                     /gene="ghoS"
                     /locus_tag="ACHUOY_003617"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0684275.1"
                     /translation="ADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDL
                     NIMTVDDYFRQFYK"
BASE COUNT         1019 a          899 c          985 g         1121 t
ORIGIN      
        1 gaccgaagaa gtttacctcg acgaaatggt aggtcgtatt aacgccaata tgatccttcc
       61 gtatccgccg ggagttcctc tggtaatgcc gggtgaaatg atcaccgaag aaagccgtcc
      121 ggttctggag ttcctgcaga tgctgtgtga aatcggcact cactatccgg gatttgaaac
      181 cgatattcac ggtgcatacc gtcaggctga tggccgctat accgttaagg tattgaaaga
      241 agaaagcaaa aaataattag ctcgtacaag ggaagtggct tgccacttcc cttttttgct
      301 cataaggaga acacatgaaa acaccctcac agccgcgcgc gatatactat atcgtggcga
      361 tccaaatctg ggagtacttc agtttttacg gcatgcgtgc cttactcatt ctctatctca
      421 cccatcagct tggttttgat gataaccatg ccatcagcct gttcagcgca tatgcttctc
      481 tggtttacgt tacccctatt ctcggcggct ggcttgccga ccgcctgctc ggcaaccgca
      541 ctgcagtgat tgccggcgcg ctgttaatga cccttggcca tgtggtgctg ggcattgata
      601 caaattcaac ctttagcctg tatctggcgc tggcaatcat tatttgtggc tacggtttat
      661 tcaaatcaaa catcagctgt ttgcttggcg agctctacga cgagaacgat catcgacgtg
      721 atggcggttt ttcgctgctg tatgctgcgg gcaatatcgg ttctatcgcc gccccaatcg
      781 cctgcggcct ggctgctcag tggtatggat ggcatgttgg ctttgccctt gcgggtggcg
      841 gcatgtttat cggtttgttg attttcttaa gcggtcatcg tcatttccag tccacgcgta
      901 gtatggataa aaaagcgctc actagcgtca aatttgcctt accagtatgg agctggttag
      961 tggtgatgct ctgtttagcc ccagtatttt ttaccctgct gctggagaac gactggtccg
     1021 gatatttgct ggcgatcgtt tgcctcattg ccgcacaaat cattgcccgc atgatgatca
     1081 aattccccga acaccgccgt gctctttggc aaattgtatt gttgatgttt gtcgggacgt
     1141 tgttctgggt actggcacaa cagggcggca gtaccatcag cttgtttatc gatcgctttg
     1201 tgaatcgtca ggcattcaat attgaagtac ctacagcact attccagtcg gtgaatgcaa
     1261 ttgcagtgat gctcgctggg gttgtactgg cctggctggc gtcgccagaa agccgcggca
     1321 actcaacatt gcgcgtctgg ctgaagtttg cctttggctt actgctgatg gcttgtggct
     1381 ttatgttgtt ggcatttgat gcccgacatg cagcggctga cggtcaagcg tcaatgggcg
     1441 tgatgatatc cgggttggcg ctaatgggct ttgcagaact ctttattgac ccggtggcga
     1501 ttgcgcaaat cacgcgtctg aaaatgtctg gcgtattaac cggtatttat atgctggcaa
     1561 caggcgcggt cgccaactgg ctggcaggcg tcgtggcaca gcagacgaca gagtcgcaaa
     1621 ttagcggtat ggtaattgca gcttaccagc gattcttttc tcagatggga gagtggacgt
     1681 tggcttgtgt cgcgatcatc gtcgtattgg cctttgctac ccgttttctg tttagcacgc
     1741 cgacgaatat gatacaggag agcaacgatt aactgtcgta aaaacaattg gttatgaata
     1801 aattaacttt cagttaatca cactagtaaa tcgagattaa taagtcattt aaaccatttt
     1861 gatggttatt tattagtgat atcaacttga ggtaagcgtt agtttcgata agataaactg
     1921 agttattaat agtcgaggca gataatacag tgtaccgatc tgtctcttta tctacgctaa
     1981 attgaaagct ggatttagag gaaccaaaat gtctgaacaa gaaacacggg gagccaatga
     2041 ggctattgat tttaacgatg aactgagaaa tcgccgcgaa aaactggcgg cactacgtca
     2101 gcaaggtgtg gcgtttccca atgattttcg ccgcgaccat acctctgacc agttgcacga
     2161 agagtttgat gcgaagaata accaggaact ggaatcctta aacattgaag tctcggttgc
     2221 tggccgaatg atgacccgtc gtatcatggg gaaagcctcc tttgtaacgt tgcaggatgt
     2281 cggtggccgt attcaactgt acgttgcaag agatagcctg ccagaaggtg tttataacga
     2341 tcagtttaaa aaatgggatc tgggtgacat tatcggtgcc cgcggtacgc tgtttaagac
     2401 gcaaacgggt gagctttcca ttcactgtac tgagttgcgc ctgctgacta aagcactacg
     2461 tcctttacca gataaattcc atggtctgca ggatcaggaa gtccgttatc gtcaacgtta
     2521 tctggacctc atcgctaacg ataaatcccg tcaaacgttt gttgtccgtt caaaaattct
     2581 ggccgctatc cgtcagttca tggtcgcgcg cggctttatg gaagtagaaa ccccgatgat
     2641 gcaggtaatt ccaggtgggg catctgctcg cccgtttatt acccatcata atgctctgga
     2701 tttagatatg tatctgcgta tcgcgccgga gctgtatctg aaacgtctgg ttgtaggcgg
     2761 ttttgaacgg gtattcgaaa tcaaccgtaa cttccgtaat gaaggtattt ctgttcgcca
     2821 taatcctgag ttcacaatga tggaactcta catggcgtat gcggattacc acgatttgat
     2881 tgaactgaca gagtcactgt tccgcaccct ggcacaagag gttctgggta ccactaaagt
     2941 cacttatggc gagcatgtgt ttgatttcgg caaaccgttt gaaaaactca ccatgcgcga
     3001 agcaatcaaa aaatatcgtc cagaaaccga tatggccgac ctggataatt ttgatgctgc
     3061 taaagcatta gctgaatcta tcggaattac ggtagagaaa agctgggggc tgggacgtat
     3121 tatcacggag atctttgatg aagtggcaga agcacatctg attcagccaa cctttattac
     3181 ggaatatccg gcagaagtgt caccgctggc gcgccgtaat gatgttaacc cggaaatcac
     3241 cgaccgtttt gaattcttca tcggtggtcg tgaaatcggt aatggtttta gcgaattaaa
     3301 cgacgcagaa gatcaggctg aacgtttcca ggaacaggtt aacgctaaag ctgcaggtga
     3361 cgacgaagcc atgttctatg acgaagatta cgtaactgcg ctggaatatg gtctgccgcc
     3421 aaccgctggt ctgggtattg gtatcgaccg aatgattatg ctgtttacaa acagtcatac
     3481 tattcgcgac gttattctct tcccggcgat gcgcccacag aaataaattt cactttaatg
     3541 aacgaagcag tcaggcgact gcttcgttcg tcgttccgca aatccagttt tgaggagaga
     3601 cgcttcgttt cacgataacc agcgggtcca ctcaactaca gctcctaaaa gagagaaaaa
     3661 agtaatgcca caggcagact cattggccag gttattccga ccaggaatgc actaagtaaa
     3721 cgaatatgtg ttttctcatg tgagataaac cagataatga caaaggatat gttcacacca
     3781 atcacataaa aaattaatat tttagagaat agtgccattt tttatgttct tcttatcctt
     3841 cctggctact tgtaaaactg acggaaatag tcatcaactg tcataatgtt gaggtcaaaa
     3901 tcaatatccg gtaagtctaa gcatttaagc ctgattaaat cttcgatttc acttgcggct
     3961 aacgatgtcg ttattgcgaa ggaattttca ttcaattgtc gtggtatccc atattcatca
     4021 gcca
//
LOCUS       DAZOYL010000309         3998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZOYL010000309 DAZOYL010000000
VERSION     DAZOYL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.310"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHUOY_003618"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHUOY_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR0684276.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUOY_003619"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUOY_003619"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR0684277.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUOY_003620"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUOY_003620"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR0684278.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUOY_003621"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUOY_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR0684279.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          953 a         1130 c          957 g          958 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacaca
//
LOCUS       DAZOYL010000310         3990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZOYL010000310 DAZOYL010000000
VERSION     DAZOYL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.311"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1075
                     /gene="ompC"
                     /locus_tag="ACHUOY_003622"
     CDS             <1..1075
                     /gene="ompC"
                     /locus_tag="ACHUOY_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0684280.1"
                     /translation="LTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFS
                     SNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGL
                     KFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFF
                     GLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQVIY
                     GNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQ
                     YQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDK
                     SDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1264..1647)
                     /gene="quuD"
                     /locus_tag="ACHUOY_003623"
     CDS             complement(1264..1647)
                     /gene="quuD"
                     /locus_tag="ACHUOY_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR0684281.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1733..1873)
                     /locus_tag="ACHUOY_003624"
     CDS             complement(1733..1873)
                     /locus_tag="ACHUOY_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR0684282.1"
                     /translation="MMFEFYMAEFLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1870..2232)
                     /gene="rusA"
                     /locus_tag="ACHUOY_003625"
     CDS             complement(1870..2232)
                     /gene="rusA"
                     /locus_tag="ACHUOY_003625"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR0684283.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2229..2519)
                     /locus_tag="ACHUOY_003626"
     CDS             complement(2229..2519)
                     /locus_tag="ACHUOY_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0684284.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2512..2682)
                     /locus_tag="ACHUOY_003627"
     CDS             complement(2512..2682)
                     /locus_tag="ACHUOY_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR0684285.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2682..3137)
                     /locus_tag="ACHUOY_003628"
     CDS             complement(2682..3137)
                     /locus_tag="ACHUOY_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR0684286.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3402..3779
                     /locus_tag="ACHUOY_003629"
     CDS             3402..3779
                     /locus_tag="ACHUOY_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684287.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT         1089 a          924 c          881 g         1096 t
ORIGIN      
        1 attaacagtg gcaatttctg ctgtagctgc atcagtactg atggcgatgt ctgctcaggc
       61 agctgaaatt tataataaag acagtaacaa gctggatctg tacgggaaag ttaatgctaa
      121 gcactacttc tcctctaatg atgcagatga tggtgatact acttatgccc gtcttggctt
      181 caaaggtgaa acccaaatca acgatcaact gactggtttc ggtcagtggg aatatgaatt
      241 caaaggcaac cgcgctgaat ctcaaggttc ctccaaagat aaaacccgtc ttgccttcgc
      301 tggcctgaaa ttcggtgact acggctccat cgattacggc cgtaactacg gtgtagcata
      361 cgacatcggt gcgtggactg acgtcctgcc agaattcggt ggtgacactt ggactcaaac
      421 agatgtgttc atgactggtc gcaccactgg tgttgcaact tatcgtaaca acgacttctt
      481 tggtctggtt gatggtctga actttgctgc tcagtatcag ggtaaaaatg accgcactga
      541 cgtaactgaa gctaatggtg atggtttcgg tttctccact acttatgagt atgaaggatt
      601 cggtgtaggt gcaacctatg ctaaatctga ccgcactaat aatcaggtta tctacggtaa
      661 caacagcctg aatgcatctg gtcaaaatgc tgaagtatgg gcagctggtc tgaaatatga
      721 tgcgaacaac atctatctgg ctaccaccta ttctgaaacc cagaacatga ctgtttttgg
      781 taataaccat attgccaaca aagcacaaaa cttcgaagta gttgcacaat atcagttcga
      841 cttcggtctg cgtccgtccg ttgcttacct gcaatctaaa ggaaaagact tgggtgcgtg
      901 gggtgatcag gacctggttg aatatattga tgtaggtgca acctattact tcaacaaaaa
      961 tatgtccact tttgttgatt acaaaatcaa cctgattgat aagagcgatt tcacgaaagc
     1021 atctggcgtt gctaccgatg atatcgttgc tgtaggtatg gtttaccagt tctaatttga
     1081 ttactaaaag atatgttgcg ggaggctttg cctccccaac atataagtgg ctccctcaag
     1141 ccacttcctt taggagcaca accttgcttc taactatata aaccttctgt tatatattac
     1201 cctttatttt tgggggcgtt gcaacgcccc atttttaata atttttagta aacaattggc
     1261 atattaatta gagttattaa caacgatatc catctctaac cggatatcta atgccattaa
     1321 catcccttca attatgccct cagccttctg taaccttttc ccgatataac catcagagca
     1381 gcaatgctta cctgccagtg acatgaatgt cataccgact acataataat ctactaataa
     1441 atcgtgcaaa tcgctgttgt tctttttcag acgggccatg cacccgcaaa taatcatcgc
     1501 gtcatcgtca caacattgcg ggcgagattt tacttttgaa gtaattaatc ccttaaaacc
     1561 ggcggcaatg gacgaccagg tcacatcttc atgattatta gccgcccacg ctccccaacg
     1621 ctcaagaacc atctgaatat cacgcatcaa cttactccac aaaaatcaga ccagaacgcc
     1681 aattacaagc aaaaatcaac aaaacagtat tagttgattg ttatctctga cttcatactc
     1741 ctgctcctgt cagggttttg gcgtaattct tcagtattcg gtaatcggtc aaaacagaac
     1801 cggggaaacg atataagcgc agatgccccc agcggtggcg aagaaattct gccatataaa
     1861 actcaaacat cattcattcc ccatttcggt gatggtcagt tccagcctcc cacctttggt
     1921 aacaggcatc ttcacaacgc ggtaatcaac gacctgagca tcatccagcc agaaacctgc
     1981 tttggtgagt gcgtcaaaag cggctttttg cagattatcc aggtcacggc gacggcgatc
     2041 cggcatgtgg cactcaatac ggattttcac tggcatagcc aggccgatat ccagcattgc
     2101 gtttttaatg attcgggtga cgttatcgcg gtatgcctgc ccttctgcgc tgatgtgtgt
     2161 gcgcccgcga ttatggcggt aatagcggtt attgctcgga ggccagggta atgtgatgct
     2221 gtaggtattc acgccttaat aaccccctct ttcagccaga taacctgcgt tctcgccata
     2281 ccttccagcg cgcattcttt tgcatactca gcatcgacaa aatatgtgcg gcggtcgatt
     2341 tcgtcgtgac aggcagaaca tgcaatggtg gcaatcaggt ctggcggttt gataccggta
     2401 ccgcacaatc cagccagccg gatatgtgcc agtactgacg tttcaggatt gccattacat
     2461 acgccaggga ttcttacctg gcattcccga ccacgcgctg cttttctcaa atcagccatg
     2521 attcctcctt gctgccagtc gcaaccattt tttatcaacc aggctagcgg tatatccgag
     2581 cagtgttggt atttcggatg gcttcagctc aggcttacgc ttacgacgat ttgatactct
     2641 gtagatgtgc ccgttcatga cacgaataag cggtgtagcc attacgcctc ctgcttatcg
     2701 cgcagctgct ggtattcaca gctctgcgga attgtcaggt ggcagccgat attcatcgcc
     2761 caggcttcga ctttgcacag gaagatgtac atctcgccgg tttccagctc tgacgtatgg
     2821 cggagggatt ggacggtggt gacctcgccg gacacgacat ctacacggtc tttgctttcg
     2881 tagccgagat aggtgtgctt catcgcgtct ttgacccact caggcgtagc gaaggtcttg
     2941 ccgcgggcga tgagatattc gctgatctcc gtgtaccaca tgtggctgag cgcgttctgc
     3001 gacaggctgc gcttctcgcg ccacggctta acctgcaggc gtaagcattg cccggcatcc
     3061 agcaatggct gaatctgctg gccgatggcc gcgaagttac cgcgatggag tttgatgccg
     3121 tctactggca gagtcatacg gcctccctaa cggaaaccgc agaatgcaga aaatcgcagg
     3181 tgcatttctg catctgtgac aaggtgatga gttcagattg tggtcgcatt taagtcccct
     3241 taaatgcgca gaagtcaccg gagttgttca ggctccgatg agaggattat ggcggattga
     3301 ttacgataaa tcaaaactat cttgaagcca aaacagtgat cttcgcttta aaaaccttgt
     3361 atactttcac ctaatcacag ccagaactat ggatctaacc aatgaccaac aaagaaaaca
     3421 aagataaata tattaacaag ttcaagaaag agctttcaaa atatatacaa ccaactgctg
     3481 gagtggacat tcaattattt aactctaagg atggtggtgg tgtaattaaa gccactttga
     3541 atagaagtgg aaaaagaaaa tcatctattg ctggtaattt tactaaatta ggtgaggcaa
     3601 ttacctcgag tgggcagagg gtttttggtg gtgatctatc aaatgtgaat ttttacggaa
     3661 caaatacgat ttttgatgga gatacaatat ttttaattaa atcacctgat tcagaggagt
     3721 ggtcatcaca aaaagcttcc aaagacgtag agggcatcgt atttggaggg aaaaaatgat
     3781 taacattact actaaggatg cactcaagtc agtggtgaag taagagatct agtaattctt
     3841 cttgaaactg atggaaaaac ggtgtctgat tatttgcagc caatccctcc caaaaaagat
     3901 acacactgga gcatgatttt cattccggta gcaatttata tactacttaa tcttgtatac
     3961 attattgctc acccattatt tcctgaaaaa
//
LOCUS       DAZOYL010000311         3969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZOYL010000311 DAZOYL010000000
VERSION     DAZOYL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.312"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            405..632
                     /gene="feoA"
                     /locus_tag="ACHUOY_003630"
     CDS             405..632
                     /gene="feoA"
                     /locus_tag="ACHUOY_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR0684288.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            649..2970
                     /gene="feoB"
                     /locus_tag="ACHUOY_003631"
     CDS             649..2970
                     /gene="feoB"
                     /locus_tag="ACHUOY_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR0684289.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2970..3206
                     /gene="feoC"
                     /locus_tag="ACHUOY_003632"
     CDS             2970..3206
                     /gene="feoC"
                     /locus_tag="ACHUOY_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR0684290.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3409..>3969
                     /locus_tag="ACHUOY_003633"
     CDS             3409..>3969
                     /locus_tag="ACHUOY_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0684291.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRD"
BASE COUNT          917 a         1040 c         1006 g         1006 t
ORIGIN      
        1 ggcgttcacg ccgcatctgg caacgaacgc cttgcctgac ataaaagtgc cggagaatat
       61 ctccggcctt tttattccac aaccaaactc ataatatatt ccggcaatat ttatcacttc
      121 attaacaact gaaaccttaa ttaaacatta gccagtccgg gtaattcact attcgaatta
      181 tattttcgct gcgatataac cttgagccac atcaacattg agtcagatta ttattcaaac
      241 caacattcgc acacatttta agtattgctg atagaaacca ttctcattat cattgtgttg
      301 ttgattattt aatctctcct ttgttggcaa atcatctggt ctcatgtcgc tgtcaaacgc
      361 cccatgaggt agttatccag ttaatgagaa acaagtaggc acctatgcaa tacactccag
      421 atactgcgtg gaaaatcact ggcttttccc gtgaaatcag cccggcatat cgccaaaaac
      481 tgctttctct tggcatgtta cctggctcct cttttaatgt ggtgcgcgtc gctccactcg
      541 gcgaccccat tcatatcgaa acccgtcgtg tgagcctggt attacgcaaa aaagatctgg
      601 ccttattaga agtggaagcg gtttcctgtt aatacggtga taacaacaat gaaaaaatta
      661 accattggct taattggtaa tccaaattct ggcaagacaa ccttatttaa ccagctcact
      721 agcgcacgtc agcgtgtagg taactgggct ggcgttaccg tcgaacgtaa agaagggcaa
      781 ttctccacca ccgatcatca ggtcacgctg gtggacctgc ccggcaccta ttctctgacc
      841 accatctcat cgcagacctc gctcgatgag caaatcgcct gtcactacat tttgagtggc
      901 gacgccgacc tgctgattaa cgtggtggat gcgtctaacc ttgagcgtaa cctgtacctg
      961 acgctacaac tgctggaact cggcattccc tgcatcgtgg cactgaacat gctcgacatt
     1021 gccgagaagc aaaatattcg tattgaaatt gatgctctgt cggcgcgtct tggctgccca
     1081 gtgatcccgc tggtttcaac ccgtggtcgc ggtattgaag cgctcaagct ggcgattgat
     1141 cgctataaag ctaacgagaa tgtggaactg gtgcattacg cacagccgct actcaacgaa
     1201 gcagattcac tggcaaaagt gatgccttcc gacatcccgc tgaaacaacg tcgctggctg
     1261 ggcctgcaaa tgctggaagg cgatatctac agccgcgcct acgccggtga agcgtcgcag
     1321 catctggatg ccgcccttgc ccgtctgcgt aatgaaatgg atgatccggc gctgcacatt
     1381 gccgatgcgc gttaccagtg catagctgcc atctgtgatg tggtaagcaa caccctgacg
     1441 gcagaaccca gccgtttcac cactgcggta gataaaatcg tgctcaaccg tttcctcggt
     1501 ctgccgattt tcctctttgt gatgtacctg atgttcctgc tggctatcaa catcggcggg
     1561 gcgttacagc cgctgtttga cgtcggctcc gtggcgctat ttgtgcatgg tattcaatgg
     1621 attggctaca cgctccactt cccggactgg ctgactatct tcctcgccca gggcctgggt
     1681 ggcggtatta acaccgtgct gccactggtg ccgcagatcg gcatgatgta cctgttcctc
     1741 tccttccttg aggactccgg ctatatggcg cgtgcagcgt ttgtgatgga ccgcctgatg
     1801 caggcgctgg gcttgccggg gaaatccttt gtgccgctga tcgtcggttt cggttgtaac
     1861 gtaccgtcgg taatgggcgc acgtacgctt gatgcaccgc gtgaacgtct gatgaccatc
     1921 atgatggcac cgtttatgtc ctgcggcgcg cgtctggcta tcttcgcagt attcgcggct
     1981 gccttcttcg ggcagaacgg tgccctggcg gttttctcgc tgtatatgct cggtatcgtg
     2041 atggcggtgc tgactggcct gatgctcaag tacaccatca tgcgcggtga agcgacgccg
     2101 tttgtcatgg agctgccggt ctaccatgta ccacacgtta aaagcctgat tatccagacc
     2161 tggcagcgtc tgaagggctt cgttctgcgt gctggtaaag tgattatcat cgtcagcatt
     2221 ttcctgagcg ctttcaacag cttctcgttg agcgggaaaa tcgtcgataa catcaacgac
     2281 tcggcgctgg cgtccgtcag ccgggtgata acccctgtct tcaagccgat tggcgtacat
     2341 gaagataact ggcaggcaac ggttggcctg tttacgggcg caatggcaaa agaagtggtg
     2401 gtgggtacgc tcaacaccct ctacaccgca gaaaatattc aggacgaaga gttcaatccg
     2461 gcggaattca acctcggtga agagctgttc agtgcggtag atgaaacctg gcagagcctg
     2521 aaagacacct tcagccttag cgtactgatg aaccccattg aagctagcaa aggcgacggt
     2581 gaaatgggta ccggggcgat gggcgtgatg gatcagaaat tcggtagcgc agctgccgca
     2641 tacagctacc tgattttcgt cctgctgtat gtaccatgta tctcggtgat gggagccatc
     2701 gcccgtgaat caagccgtgg ctggatgggc ttctccatcc tgtgggggct gaatatcgct
     2761 tactcactgg caacattgtt ctatcaggtc gccagctaca gtcagcaccc aacttacagc
     2821 ctggtgtgca ttctggcggt aatcctgttt aacatcgtag ttatcggtct gctgcgccgc
     2881 gcgcgtagcc gggtggatat cgaactgctg gcaacccgca agtcggtaag cagttgctgc
     2941 gcagccagca ccaccggtga ttgccattaa tggcttcact tattcaggta cgtgatttgc
     3001 tggcgttacg gggccgtatg gaagcgaccc agataagcca gacattgaac actccgcagc
     3061 caatgattaa cgccatgctg caacaactgg aaagtatggg caaagccgtg cggattcagg
     3121 aagaacctga cggctgcctc tctggcagtt gtaaaagctg cccggaagga aaagcctgtc
     3181 tgcgcgagtg gtgggcgctg cgttaacctt actccatcgc ctgttttgga aagcccggta
     3241 tgcgtctgca tccgggcttt tttgcgtgcg gctttccata aaaatgcaac tcttgcagca
     3301 cggcgtaagt tcctttgaaa gcatctcgca gggatgaaaa ctcgctaatg cacaggtgtg
     3361 gagtggcgcg tagagtcgcg gcattcaaac aacaggtgaa ggaacgccat gagcaaaaag
     3421 cagagttcca ccccacacga tgcgctgttc aaactctttt tacgccaacc ggacacggct
     3481 cgtgattttc ttgcgtttca tttaccggca cccattcacg cgctttgtga tatgaaaacc
     3541 ctcaagctgg agtcgagcag ctttattgat gacgatctgc gtgaaagcta ttccgatgtg
     3601 ctgtggtcgg tgaaaacgga acaaggacca ggatacatct attgtctgat tgaacatcaa
     3661 agcacctcaa acaaactgat cgcatttcgc atgatgcgtt acgctattgc cgcaatgcaa
     3721 aatcaccttg atgctggata caaaacgttg ccgatggtgg tgctattgtt gttttaccac
     3781 ggtattgaaa gcccctatcc ctattcgctg tgttggctgg attgtttcgc cgatcccaaa
     3841 ctggcaaggc agctttatgc ctccgcattt ccgctgattg atgtcaccgt catgcctgat
     3901 gatgaaatca tgcagcaccg acgtatggcg ctgctggagt taattcaaaa acatattcgt
     3961 caacgcgac
//
LOCUS       DAZOYL010000312         3961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZOYL010000312 DAZOYL010000000
VERSION     DAZOYL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.313"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..76)
                     /locus_tag="ACHUOY_003634"
     tRNA            complement(1..76)
                     /locus_tag="ACHUOY_003634"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Thr,seq:tgt)
     gene            complement(79..155)
                     /locus_tag="ACHUOY_003635"
     tRNA            complement(79..155)
                     /locus_tag="ACHUOY_003635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Arg,seq:tct)
     gene            complement(157..233)
                     /locus_tag="ACHUOY_003636"
     tRNA            complement(157..233)
                     /locus_tag="ACHUOY_003636"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ile,seq:cat)
     gene            complement(423..986)
                     /locus_tag="ACHUOY_003637"
     CDS             complement(423..986)
                     /locus_tag="ACHUOY_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0684292.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1090..1308)
                     /locus_tag="ACHUOY_003638"
     CDS             complement(1090..1308)
                     /locus_tag="ACHUOY_003638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684293.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1467..1853)
                     /locus_tag="ACHUOY_003639"
     CDS             complement(1467..1853)
                     /locus_tag="ACHUOY_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR0684294.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1843..2214)
                     /locus_tag="ACHUOY_003640"
     CDS             complement(1843..2214)
                     /locus_tag="ACHUOY_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0684295.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(2214..2471)
                     /locus_tag="ACHUOY_003641"
     CDS             complement(2214..2471)
                     /locus_tag="ACHUOY_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684296.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2468..3859)
                     /locus_tag="ACHUOY_003642"
     CDS             complement(2468..3859)
                     /locus_tag="ACHUOY_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0684297.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3856..>3961)
                     /locus_tag="ACHUOY_003643"
     CDS             complement(3856..>3961)
                     /locus_tag="ACHUOY_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0684298.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          930 a         1105 c          895 g         1031 t
ORIGIN      
        1 tggtgccgac taccggaatc gaactggtga cctactgatt acaagtcagt tgctctgcct
       61 gctgagctaa gtcggcgctg gcccaccacc gaggactcga acctcgcacc gtcaacttag
      121 aaggttgatg ctctatccgg atgagctagt ggtggttggt ggcccttgct ggacttgaac
      181 cagcgacctg gcgattatga gtcgctcgct ctgaccaact gagctaaagg gccggaggca
      241 gaataataac catatgtcat cacatctgca aactcatctg accaccagca cgtttaacgt
      301 cctgtgccgt ttttcaggta taaaaaaacc cgctcatcgg cgggtttaag ctgtgtggcg
      361 tagtaaccac tcttaacagg atattcaact ttttacgatc gtaaagtttt cggaaaaaat
      421 ttttaaaacc gcatcaaccg ctccaccagt tgctctttac gggcaacgat ccacccgtgt
      481 tgctccagat aaaatttaaa ccgttccaga gtacatacca tcgcatcggc gggtaccttt
      541 tccgtgaact caacctgacc gtgtttatcg aagtggctca gtaatgcgca tccatcattt
      601 tcgatagatg tgttttgtgc cgctggtggc tgtttctggt tgaaataaca gtcttccagt
      661 ttttcgaaca cttcccacgc ctgatcggtt tcgagcattt tggcgtgacg ggctgcgcca
      721 cgttctgtcc agaggatgag ggagcgaacg ttacgggcaa ttttcacaga aactcttaaa
      781 gagttttcgt tcttcaactc tcgtaacgca tcaccttcta tcaggaaaaa atgtttacct
      841 tctataaacc gttctttgtt tcgattgaaa ttgtttgtca aacgctggcg ctctgttccg
      901 tacaaatcag ccagcagctc tgttgtcatg acaggaagat ggttatgcgt aagtgacggg
      961 agtttttcaa tagacgtgcg attcatagac atgtcctttc gaatattttg atttacccct
     1021 tttgagaggg tgaccgggcg ctcaaaaccg tcgaaagtcg gcgggcgtat ttccattgct
     1081 ggtattgtat tagccgcaca cccggtcata aaccaagaat tctggacaca aaaaaaccac
     1141 atggctatcg ggtgtggata ccgctttcga ggtgttttga gcaccgcggg gaaatgtaaa
     1201 ctgcaaacct gaaactgtca acaccgtcag gttccaatca tcgggtggtg agacgcacag
     1261 ggttggaact accgggaaac cgaccggcga gcttttcagc tcccccatgc gccccaccat
     1321 aattcagatg tgcgcgtgca tacgacaata aaaaacacgc tcgcggcgtg tgtctgtcgc
     1381 ggtctctatc cggggttcca atcccgacgc cagattttgc tggcgcgtga ggaatatagc
     1441 cccggacaat gtgtctggtc aagcacctac ataattcgtt ctacgtatct gtccatctcc
     1501 agccggatat caagcatcat caacatgcca tcaataaccc cttccgcttt ctgcaggcgc
     1561 ttgccaatac aggtatccga acacccatgt ttccgtgcca gactcataaa agtcattcca
     1621 cctacgtaat aatccaccag caaatcgtgc aaatcctgat ttttcttgtt caaccgggcc
     1681 atacagccac aaattatcat tgcatcatca tcagaacact gagggcgtga tttcactttc
     1741 gacggaatta accctttaaa accagcagcg atcgacgccc aggatacatc ttcatgagtg
     1801 tttgcagccc aagcccccca ccgttccata acctgctgaa tatcacgcgc catcgttatc
     1861 acctgtgatt tcataaatct tcacgcccag tcgcccacca ggaacgagct gaccgcgcac
     1921 aatattgatt tcatcaaact gctcgtcgtc tatgagtagt ccggcatgcg tcagcgcatc
     1981 cagtggtgct ttcaggatat tgtccaggtc acgacggcgc ttatccggtg gctctgcaat
     2041 aatctttatc gccagccttc cggacaggtt taatttcagc cgctgctggc gaacaattag
     2101 cgtcacatca cggcgataac gctcaccaac ttttgataca aaatatgtgc tgccacgacg
     2161 tcgccagtaa gtattcaccg tcggcggata aggtaaaata aactcatggc gcatcagcgc
     2221 agcacctcct gcaccatttt ttcaaacttt ccaactttgg tttccagctc tgccacacaa
     2281 tcaacgagct catccaccgc tttttgtgcg cgatgtttcg cctgcatcag ttccctgagc
     2341 gctggcacca tatcccgacg aatggcatct tttgttacac ctgttttttc cagttgttcc
     2401 gcctgtcgca acatttcctg cgcgtgttta cgcaattgtt cagaggtaaa agtcattgtc
     2461 tggttgttca aaagaaacgc tccatcttac tgctgtcggt acgtgtattg ctgtatctgc
     2521 gcggctgggg ctgccgcatt ggggtggaaa gaacctgtgc gctttcctgg tccacaggca
     2581 gaaaatgtcc gttataaaaa cgccggtaaa tcgtccccag agaaccgtta cgttgtttcg
     2641 tgatattaat ttctgctatg cctgtagcct gtgtatccgg gttgtacact tcatccctgt
     2701 aaagcatcag aatgatgtct gcatccgcct ctatttctcc ggaatttttc aggtctgagt
     2761 tcatgggacg tttattgggt ctggactcca caccgcggga gagctggctc agcgcaatca
     2821 acggaaaacc accggatttt gccaggcctt taagcccctt tgagatttca cccacggcaa
     2881 ggtcatgacg ccccgtggtt cgggttttta tcagcccgag ataatcaacc accaccagcg
     2941 ccgtttccgg atgtttaatc agatggtgtt tcgttgttgc gcatatctca tcaatggtca
     3001 ggttcgcctg gtccaccatc cagatattgc gcccggtcat ccgccccacc ccttgtgaga
     3061 aacgcgccca gtcttcatct tcaaagtgag ccacagattt caggcgtgat actggcatcc
     3121 ctccagccgc agacaccata cgttcaccaa tctggatgtt cgccatctcc atggtgaaca
     3181 gaagcacacc atgcccctgc tcagtcacct tgtcgatgat gtccagcgca agttcggttt
     3241 tccccatcga aggacgggcg gcaatgaata ccaggtctcc gggctccata ccgcctgttt
     3301 ttgcgtccag ttcatcaata ccggtcatca acgtcctgga tttctccagc ccctgattcc
     3361 ggcattcaac acgctcaacc acttccggaa gcacatcatc aatatgtacc ggctgaatga
     3421 cgccctttcc tgtcgacagt gtgaccatca tgttctgcgc atccttcagg gcatcttcag
     3481 ctgcttcaca ggtatgcgca tcacgtaatt tctgcagcgc ctcattcagt gttttttctg
     3541 catcgcgcag tgcggcattg cgccgcaacg ctgcaacata gtgctccagt gaagacttca
     3601 cccaggtttt acgcccggta tcagtaatca ccggggcaag ttccggcatc tcattacaca
     3661 acagcacggg gtcaatcact cctgaaacac gggcctgtct gcagatgcct gtgtagatat
     3721 cccgatacgc tcgtacagaa aaaatgtccg ccggtagtgt gatcagaata tccatcactt
     3781 catgatctgc cccacgcaga aagaatgcgc caatgacagc gccttccagg tcatcattac
     3841 gccagactgg tgttgtcatg cagccacacc tctgatacaa gaacggtagc tgggccagtt
     3901 gaacgacaac cagttgcgcc ccccgtctgt gatcctgtcg gcaatgcggg ggctgatgaa
     3961 c
//
LOCUS       DAZOYL010000313         3913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZOYL010000313 DAZOYL010000000
VERSION     DAZOYL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.314"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..2601)
                     /gene="acrF"
                     /locus_tag="ACHUOY_003644"
                     /pseudo
     CDS             complement(1..2601)
                     /gene="acrF"
                     /locus_tag="ACHUOY_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="ACHUOY_003645"
     CDS             complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="ACHUOY_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR0684299.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT          944 a         1035 c          973 g          961 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac
      961 aatcagcgca tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt
     1021 ggtgtagtgg ttaacgctat gatcgaaggt ggtattaaac cagccgaaga aaccgccctt
     1081 attttcgtga tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag
     1141 aatcaatgcc accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg
     1201 ataaattgcc ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac
     1261 catcgcgata cccaccagtg cgccctgaat ttgcgacatc gatttttccg tcgcttcttt
     1321 tggcgggagc ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc
     1381 atcgacgagc agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta
     1441 accaaaagcg gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg
     1501 gatcagcgtt gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat
     1561 ggcttcgaac agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc
     1621 ataagggtag agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc
     1681 cttaatggct ttcgcggtat tgagagcgtt cgcgccggtt gctagcttaa tccccaggcc
     1741 cgccgccggt tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac
     1801 tcgtgcgaca tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggttacttt
     1861 gccgaattct tccggatttt ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg
     1921 ccctggtaac gctggcgttc cgcccaactg cccggcagcg atctgatcgt tctgtacctt
     1981 caactggtta atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag
     2041 ccagatacgc atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg
     2101 gctaagcgta tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc
     2161 tgggttatca gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact
     2221 gatcccctgc tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg
     2281 cacctgcact tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac
     2341 gctaccggcg gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt
     2401 ctgttcgata acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt
     2461 tgcctaaaca gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag
     2521 gatcgccagt gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg
     2581 tcgaataaaa aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta
     2641 tcggtagtgg ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact
     2701 tgatcgccag atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca
     2761 acgacagggc gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca
     2821 cgcggtgtgc ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca
     2881 tcaatccgtg cacgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagaca
     2941 gcacgtaggg ttatggagcc ggtgcattca tcaacggtca catcagagaa ttgcagcgta
     3001 cctttcaggg gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt
     3061 tccttatgca aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat
     3121 tgggtcacat caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc
     3181 ccattagtga caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc
     3241 gcagtcactt tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca
     3301 atcacggcgg catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg
     3361 atgtatttcg tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg
     3421 gcggcttcac ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg
     3481 ggatcgatct ggtacaggga ctggcctgct tgtacatcgc tgccttcagt gaaattacga
     3541 ttcagtacga tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg
     3601 cctggtaatt cagtcttaac ttctaacggg gccgttttca caatatgaac ggtaacctgc
     3661 ggttcaccga cgtgagcttt ctcttctccc ttatcgttac aaccgacgat taaagccgcg
     3721 gagatcagaa taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct
     3781 caaaaaaaca aaagcgcgtt atttacccag aaaggcaacg cgttaactcg cagaaagaaa
     3841 aatacagttt gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaatact
     3901 ggcgtctcaa ggc
//
LOCUS       DAZOYL010000314         3909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZOYL010000314 DAZOYL010000000
VERSION     DAZOYL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.315"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            140..1090
                     /gene="tal"
                     /locus_tag="ACHUOY_003646"
     CDS             140..1090
                     /gene="tal"
                     /locus_tag="ACHUOY_003646"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0684300.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            1110..2656
                     /locus_tag="ACHUOY_003647"
                     /pseudo
     CDS             1110..2656
                     /locus_tag="ACHUOY_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
     gene            complement(2752..3795)
                     /locus_tag="ACHUOY_003648"
     CDS             complement(2752..3795)
                     /locus_tag="ACHUOY_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR0684301.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(3689..>3909)
                     /locus_tag="ACHUOY_003649"
     CDS             complement(3689..>3909)
                     /locus_tag="ACHUOY_003649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684302.1"
                     /translation="YFVVYYWLNPTNLIEPRYLRNEDTTCAVCLNFWGRTVHALSHFP
                     SLFFRFVADVFGVGGTSATGVFRLAGHL"
BASE COUNT          953 a         1027 c         1046 g          883 t
ORIGIN      
        1 cctataacac tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt
       61 ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac
      121 ttttcaagga gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt
      181 ggcagacagc ggcgatattg agtccattcg ccattatcat ccccaggatg ccaccaccaa
      241 tccttcgctg ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc
      301 tatcgcctgg ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga
      361 caaactggcg gtcaatttcg gtgctgaaat cctcaaaatc gtacccggtc gcgtgtcaac
      421 agaagttgat gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct
      481 ggtggacttg tatcagcaac aaggcgttga gaaatcacgc attctgatca agctggcttc
      541 gacctgggaa ggaattcgcg cggcagaaga gctggaaaaa gaaggtatta actgcaacct
      601 gacgctgctg ttttcttttg cacaggcacg ggcctgtgcg gaagcaggcg tttttctgat
      661 ttcgccgttt gtcgggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta
      721 tgtggtggaa gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca
      781 acaccactat gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc
      841 cttaaccggc tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa
      901 agtttcgcca gtggtacgta aattaatccc accttctcag acgttcccac gcccagctcc
      961 catgagcgaa gcggagttcc gttgggagca caatcagtat gcgatggcgg tagaaaaact
     1021 gtctgaaggc attcgtctgt tcgccgttga tcaacgcaaa ctggaagatc ttcttgccgc
     1081 caaactataa accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc
     1141 gcgcactcag tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg
     1201 gcatggctga tattgccgaa gtgctgtgga acgattttct taaacataac cctaccgacc
     1261 caacctggta tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct
     1321 acagtttgct gcatctgacc ggttacgacc tgccgctgga agagctgaag aactttcgtc
     1381 aactgcattc gaaaacccca ggccacccgg agattggtta tacgccaggc gttgaaacca
     1441 ccaccggtcc acttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta
     1501 cactggcggc gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt
     1561 ttatgggcga cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca
     1621 cgctgggact gggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg
     1681 aaacagaagg ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg
     1741 tgatccatga aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc
     1801 aaagcgtgaa agataagccg tcgctgatta tctgtcgtac ggtgattggc tttggatcgc
     1861 cgaataaagc aggtaaggaa gaggcgcacg gcgcaccgct gggggaagaa gaagtggcgc
     1921 tggcacggca aaaactgggc tggcaccatc cgccatgttt gttgaatacg cccgtaacgc
     1981 cgcgcggatg gcggcactga tgaaagcgcg gcagattatg gtttataccc acgactcaat
     2041 tggcctgggc gaagatggtc cgacgcacca ggctgttgag caactggcca gcctgcgctt
     2101 aacgccaaat ttcagcacct ggcgaccgtg cgatcaggtg gaagcggcgg tgggctggaa
     2161 gctggcggtt gagcgccaca acggaccgac ggcactgatc ctctcaaggc agaatctggc
     2221 ccaggtggaa cgtacgccgg atcaggttaa agagattact cgtggcggtt atgtgctgaa
     2281 agacagcggc ggtaagccag atattattct gattgccacc ggttcagaga tggaaatcac
     2341 cctgcaagct gcggagaaat tagcaggaga aggtcgcaat gttcgcgtgg tttccctgcc
     2401 ctcgaccgat attttcgacg cccaggatga ggaatatcgg gagtcggtgt tgccttctaa
     2461 cgttgcggct cgcgtggcgg tggaagcagg tattgccgat tactggtaca agtatgttgg
     2521 tctgaaaggg gcaattgtcg ggatgacggg ttatggggaa tctgctccgg cggataagct
     2581 gttcccgttc tttggcttta ccgccgagaa tattgtggca aaagcgcata aggtgctggg
     2641 agtgaaaggt gcctgatagt gatcgccgga tgctgattgc cggatgcgac gctgacgcgt
     2701 cttatccggc ctacagcgtc ttatccggcc tacatgtccc cgccattttg tttaacgggt
     2761 gatccacaac gtgggccagg cgtctggccc atgccagtta tcgcaggtgg gttctgcggc
     2821 gtaacgcacc aggcgaaaac gctgaccgtc aaagcgccag cgcgcctgaa tgccacaatc
     2881 gcttaatccg cgccctttcg ctaaggtcac cagttcacgc gatttctcat caaatgttgc
     2941 gttcatcagt tccagttcat tcgtctcctg cccgttgttg aacggcaaac gcaaccgaac
     3001 cgggcgcgag gctagcggct ttttacgcga cacaatccat gccaaatcaa tggtgttata
     3061 ggcccctgcc tcacagctga tcatcatcag cgctttatca tcagtcagcg cagtgacatt
     3121 cacctcacga cgtaacggat caagcgagca gcgcagacca ttcatccgcc agtttccata
     3181 atccagcaaa tcgttgcgtt cttcgagtga gagtggcgtc ggcgttgggt taaccaccgc
     3241 gacctctttc agcgcaggcg cgggcggtac gctgagcggc ggttcgtccc ctttcttgat
     3301 ccacgcggtt tcactgccaa cgcgcttttg ctgagcatca ataaacaaca acgccgcttt
     3361 taagccactc agagaaatgg tctgattgcc gtcgcgtaag gtgatcgcct tcccttcctg
     3421 aatcatctgc aaaaacgcgg tgatggttgc cgtatcatcg gttactaata accatggtga
     3481 aatccgccac ttgtcaccac ttaacgccaa cggctcacca tctaacagca gccgtggcgc
     3541 tatctctcct tcagacgcat ccggcgactt caacccgccg cgctcaatac gtaaaacggc
     3601 atcggtatgc gccccggcgc tgcggctcag ggtcatcacc agtccattat gatcgcccgt
     3661 attgcgcgcc acgcagaagt tttggttatt acaggtgacc tgccagtcgg aaaacgcccg
     3721 ttgcgctagt gccgcccaca ccaaagacgt cggcaacaaa gcgaaaaaaa agagaaggaa
     3781 aatgcgatag cgcatggacg gtacgacccc agaagttcaa acaaacagca caagtcgtat
     3841 cttcgttgcg caggtagcgc ggctcaatca gatttgtcgg attgagccaa taataaacaa
     3901 cgaaatatt
//
LOCUS       DAZOYL010000315         3908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZOYL010000315 DAZOYL010000000
VERSION     DAZOYL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.316"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            24..590
                     /locus_tag="ACHUOY_003650"
     CDS             24..590
                     /locus_tag="ACHUOY_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR0684303.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="ACHUOY_003651"
     CDS             594..1169
                     /locus_tag="ACHUOY_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR0684304.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="ACHUOY_003652"
     CDS             complement(1211..2263)
                     /locus_tag="ACHUOY_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR0684305.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUOY_003653"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUOY_003653"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR0684306.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3765
                     /locus_tag="ACHUOY_003654"
                     /pseudo
     CDS             3580..3765
                     /locus_tag="ACHUOY_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          987 c          951 g          999 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAZOYL010000316         3858 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZOYL010000316 DAZOYL010000000
VERSION     DAZOYL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.317"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="ACHUOY_003655"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="ACHUOY_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR0684307.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHUOY_003656"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHUOY_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR0684308.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHUOY_003657"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHUOY_003657"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR0684309.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHUOY_003658"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHUOY_003658"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR0684310.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          911 a         1049 c          942 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggccagac
//
LOCUS       DAZOYL010000317         3836 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZOYL010000317 DAZOYL010000000
VERSION     DAZOYL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.318"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            60..335
                     /gene="ypdI"
                     /locus_tag="ACHUOY_003659"
     CDS             60..335
                     /gene="ypdI"
                     /locus_tag="ACHUOY_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR0684311.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(412..654)
                     /locus_tag="ACHUOY_003660"
     CDS             complement(412..654)
                     /locus_tag="ACHUOY_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR0684312.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            1007..1927
                     /gene="lpxP"
                     /locus_tag="ACHUOY_003661"
     CDS             1007..1927
                     /gene="lpxP"
                     /locus_tag="ACHUOY_003661"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR0684313.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2283..2354
                     /gene="ypdK"
                     /locus_tag="ACHUOY_003662"
     CDS             2283..2354
                     /gene="ypdK"
                     /locus_tag="ACHUOY_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR0684314.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2419..3657)
                     /gene="alaC"
                     /locus_tag="ACHUOY_003663"
     CDS             complement(2419..3657)
                     /gene="alaC"
                     /locus_tag="ACHUOY_003663"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR0684315.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          996 a          973 c          892 g          975 t
ORIGIN      
        1 tgtaaataca gccataacat cgtatggttc tcagtgaacc attttcagga taactttcta
       61 tgaaagtaaa cttaatactt ttcagcttat ttttattggt ctctattatg gcatgcaatg
      121 tttttgcatt ttccatttct ggtggtgtaa gtgagaggag ctataaagag actgaaaaaa
      181 catcagcgat gacaaccaca cactctacaa aacttcagcc atcacaggcg attttgttta
      241 agatgagaga agatgcgcca ccattaaacc tcacagaaga aatcacgcct acttatccga
      301 caaaggcgaa ttatcttatt catcctgtgc gatagcgtct gtcacatggc aggtgaacaa
      361 tcatgtctga tgctgatgtg aacacctgcc caaatgagcc atcctgaccc actatcgcct
      421 gccatacccc accataaagc gaatcatcaa gccacaagcc ggggcaagaa acataaatat
      481 ccccgtcacc agttcattac cgccagttaa ccacatggtg aagtaataaa tgagtgcagc
      541 gaggatcacc atgccgaaga aagaagagac cgccgatacc acgttcagta cctgaccgat
      601 gtagccgctt acgcagacaa tacacagggc gagaaacatc cataaataaa tcatagctca
      661 acctcctgcc gcgctaaaaa acctgtaact tcagtcactt taatagtaag acaatcctta
      721 acctctggca atttttgcta agcatcaatg aaaacagcac gttaaatgca acgctattgc
      781 gtgcggaaaa ttttttactt tgcacgatta ataatcagta gctgaaagca gtcagccagt
      841 gataaagtag agccaccgca ttcatgctcc tcgtggttat gtcctgacta gtctttacac
      901 tctttacagg aaccattgtc gtacatgatg gcccaaccaa ttgagtgtac cgctgtcgac
      961 atggaatctt ctgcgataca acaattcgta tctacagaag gtaactatgt ttccacaatg
     1021 caaattttcc cgcgagtttc tacatcctcg ctactggctc acatggtttg ggcttggtgt
     1081 actctggctt tgggtacagc ttccttatcc tgttctctgc tttctcggca cgcgtattgg
     1141 cgcaatggcg cgaccattcc tgaaacgtcg tgaatctatc gcccgtaaaa acctggaact
     1201 ttgtttcccg cagcattctg cggaagaacg cgagaagatg attgccgaaa actttcgttc
     1261 actcggcatg gcgctggtag aaaccggcat ggcatggttc tggcccgaca gtcgcgtacg
     1321 taaatggttt gatgttgaag ggttggataa ccctaaacgc gcacaaatgc aaaatcgcgg
     1381 cgtaatggtt gtcggcgtcc attttatgtc gctggaactg ggcggccgcg tgatgggact
     1441 gtgccaacca atgatggcta cctatcgtcc acataataat cagctgatgg aatgggtgca
     1501 gacccgtggg cgcatgcgct ctaacaaagc gatgatcggc agaaataatc tgcgcggcat
     1561 tgtcggtgca ctgaagaaag gtgaagcggt atggtttgct cccgatcagg attatggtcg
     1621 taaaggcagc tccttcgcgc cgttctttgc ggtggaaaat gtcgccacaa ccaatggcac
     1681 ctatgttctc tcccgtctct ctggcgcagc catgttgacc gtaacgatgg taagaaaagc
     1741 ggattacagc ggatatcgtt tgtttatcac cccagagatg gaaggctacc cgacagatga
     1801 aaatcaagcc gctgcctata tgaacaagat tatcgagaaa gagatcatgc gcgcaccgga
     1861 gcagtacctc tggatccacc gtcgctttaa aacgcgcccg gtgggagaat cgtcgttgta
     1921 catttaacaa actgctctta ctctttccct cctcaaatcg gccataataa cctcatggtt
     1981 tttatggctt ttttcttaag gttttttcct tcgctgaata tctaattatt accgtcagaa
     2041 attaaactgt cgccgatatg agacacttgt aagttactaa aagtaaataa gaaaatgcct
     2101 cttgtctcgt ttcagtttta ggcgtacatt aacgccgtct tgagcagttt agctcagaat
     2161 tctggggtga tgcccacggt aaaatacagg gcttttaagg tcgctttgcc cggtgtcaac
     2221 tcactgtatc aggtgtaatg aagtcattca ggcgtaacag taattacgcg gagagatgta
     2281 aagtgaaata tttctttatg ggcatttctt ttatggtcat cgtttgggcc ggtacttttg
     2341 ccctgatgat ctaaaagcaa actgtgcaat gaaaaacagg agccatcagg ctcctgtttg
     2401 catttctgac gacggtgttt attccgcgtt ttcgtgaata tgtttgctgc tggcgggtag
     2461 taaaccgtcg gcacggaaca tcgctttaat ccctctgatg gcctgacgaa tacggtcgcg
     2521 gttttcaatc agtgcaaagc gaacatgcgt atcaccatag tcgccaaagc caatccccgg
     2581 cgagacacaa accttcgctt cgttaaggag cttcttggca aattccagcg atcccatggc
     2641 cgcatatggt tccgggattt tcgcccagac atacatcgaa gccttcggca tttcgaccat
     2701 ccagcccgct tcatgcagcc ctttaaccaa tacatcacgg cggcgtttgt actgttcggt
     2761 aatgtcgcgc acgcactgtt gatcgccctc gagtgccgca atcgccgcca cctgcaacgg
     2821 cgtaaaagtg ccgtaatcgt gatagctttt gattcgcgcc agggcactga ccagcgtttt
     2881 gttgccgacc ataaagccga tacgccagcc cgccatgttg tagcttttcg acagcgtaaa
     2941 gaactcgacc gccacatcgc gtgcacccgg cacctgcata attgacggcg ctttccagcc
     3001 atcgtagacg atatcggcat aggccaggtc atggaccacc agcacatcgt agcgtttcgc
     3061 cagcgccaca accttttcga agaactccag ttccacgcat tgcgcggttg ggttcgacgg
     3121 gaagccgagg atcatcatct tcggtttcgg ataactttca cgaatggcgc gctccagttc
     3181 gttgaagaaa tcgacacctt ccaccagcgg cactgagcgt acctgcgccc cggcaatcac
     3241 cgcgccataa atatggatcg ggtaacttgg attcggcacc agcaccgtgt caccatgatc
     3301 cagcgtcgcc agcatcagat gcgccaggcc ctctttcgaa ccaatagtga cgatggcttc
     3361 tgattccggg tcgatttcaa cgtcgtagcg atcctgatac cagcgggaaa tggcgcgacg
     3421 taaccgcgga atgccgcgtg aagtggagta accatgcgtg tccgggcgct gggccacagt
     3481 acataatttt tcgacgatat gcggcggagt cgcaccgtcc gggttaccca tgctgaaatc
     3541 gataatatct tcgccgcgcc gacgcgcagc cattttcagt tcagcggtga tgttaaaaac
     3601 gtagggcggg agacgatcaa tgcgcgtaaa gcgacgttca gggcgagtgt cagccataat
     3661 ttcctcagat taacgttagc gcccggaccg tccgagcgac gctgccacaa tggtggctgt
     3721 tttgaaaata gcctgattaa tttctgtctg tccagcgttt tagaacattt ttatccatgg
     3781 ctttaaatga ggaactccac ttcaaaaaca ggaaattttt ctacgctaac aagaaa
//
LOCUS       DAZOYL010000318         3805 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZOYL010000318 DAZOYL010000000
VERSION     DAZOYL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.319"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            73..732
                     /locus_tag="ACHUOY_003664"
     CDS             73..732
                     /locus_tag="ACHUOY_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR0684316.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="ACHUOY_003665"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="ACHUOY_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR0684317.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="ACHUOY_003666"
     CDS             complement(1860..2570)
                     /locus_tag="ACHUOY_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR0684318.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="ACHUOY_003667"
     CDS             3004..3294
                     /locus_tag="ACHUOY_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR0684319.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="ACHUOY_003668"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="ACHUOY_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR0684320.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
BASE COUNT         1003 a          951 c          937 g          914 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcaca
//
LOCUS       DAZOYL010000319         3803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZOYL010000319 DAZOYL010000000
VERSION     DAZOYL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.320"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /gene="chbB"
                     /locus_tag="ACHUOY_003669"
     CDS             complement(<1..251)
                     /gene="chbB"
                     /locus_tag="ACHUOY_003669"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR0684321.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLL"
     gene            complement(550..888)
                     /gene="osmE"
                     /locus_tag="ACHUOY_003670"
     CDS             complement(550..888)
                     /gene="osmE"
                     /locus_tag="ACHUOY_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR0684322.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1090..1917
                     /gene="nadE"
                     /locus_tag="ACHUOY_003671"
     CDS             1090..1917
                     /gene="nadE"
                     /locus_tag="ACHUOY_003671"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR0684323.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2147..3034
                     /gene="cho"
                     /locus_tag="ACHUOY_003672"
     CDS             2147..3034
                     /gene="cho"
                     /locus_tag="ACHUOY_003672"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR0684324.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(2994..3569)
                     /gene="ves"
                     /locus_tag="ACHUOY_003673"
     CDS             complement(2994..3569)
                     /gene="ves"
                     /locus_tag="ACHUOY_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR0684325.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLRPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT          971 a          940 c          911 g          981 t
ORIGIN      
        1 taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc
       61 aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca
      121 gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg
      181 cgcatttttg ataccagtaa agaggtagac atgcccgcag aacaaaacag ataaatgtgt
      241 ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc
      301 ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga
      361 aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa attgtttctc
      421 cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa
      481 gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc aagctttcag
      541 acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg tctgataacc
      601 ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc
      661 acgttgaccc aggatgtagg tctggcaagt accacgagca tggatcatgc tcacttcaga
      721 cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt
      781 caccacaggc tgtacaaact ggtctttagt acgatcataa gccgtacaac ccgccagcat
      841 ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct
      901 tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgcgaac
      961 gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt cagcccgctg
     1021 tcgcttgtcg tttcagtggc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg
     1081 aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt
     1141 aatgctgaag aggaaattcg tcgtagtatc gattttctga aaagctacct gcaaacttat
     1201 ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac gcttgccgga
     1261 aagctgtgcc agatggcgat taatgagctg cgcctggaaa ccggcaacga atcactgcaa
     1321 tttattgccg tacgcctgcc ctatggtgtt caggccgacg aacaagattg ccaggatgcc
     1381 attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtattggcc
     1441 agcgaacagg cattgcggga agcaggcatt gaactgagcg attttgtccg tggcaatgaa
     1501 aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag cggtgtcgtg
     1561 gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata tggtgacggc
     1621 ggtacagaca ttaatccgct gtatcgtctc aacaaacgtc agggtaaaca gttactggcg
     1681 gcattaggtt gcccggaaca cctttataag aaagcgccaa cggccgatct ggaagatgat
     1741 cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat cgacgactat
     1801 ctggaaggga aaaacgtacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa
     1861 accgaacata aacgccgtcc gccaattacc gttttcgatg atttctggaa aaagtaataa
     1921 tttgcacata atggtttgtg cgaaaaagag taatttgttc acgccggatg cggcgtgaac
     1981 gccttattcg acctacaaaa ctatgcaaat tcaatatatt gcaggagtcg aataggcctg
     2041 acaggcgtag cacgtcagac ggtgtaacct ttgtcatcga cccgcctctt ttttaatcgc
     2101 tccccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt
     2161 aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttacg
     2221 ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga
     2281 caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc tttctcattt
     2341 acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct ggatctgtac
     2401 cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac aacagccgct
     2461 gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg
     2521 cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc tgtttggcct
     2581 gtttgccaat aggcgcgcag ctttgcaagc attgcagacc atcgctgatg aacaaaaact
     2641 ttgttatggc ctgctgggac tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc
     2701 gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac atgcgctacg
     2761 cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag gtgcggtggc
     2821 actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct
     2881 gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg ccgggtttga
     2941 tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg aaattactga
     3001 acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag tttaccttgc
     3061 ggtcgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg
     3121 agcaatttat cgcccagttg ccacgccccg ttaatcacaa acaccacccc gccgcgtgag
     3181 ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgt
     3241 gtcatgatgt tgaaatccat cgacatctgc ccttccgtca gtttcgcttt taccacctgg
     3301 tccgctgcaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg gtctgcgctt
     3361 tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc gggaaacaaa
     3421 gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa atcacgtttt
     3481 gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag catttcgcca cagattcacc
     3541 gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt ttcattgtta
     3601 tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta gtggtgatca
     3661 ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt
     3721 actgcaaggt agtggacaag accagcggtc ttaagttttt tggctgaaag attattcagc
     3781 agttgcaggc attttacctt ttg
//
LOCUS       DAZOYL010000320         3785 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZOYL010000320 DAZOYL010000000
VERSION     DAZOYL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.321"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHUOY_003674"
     CDS             complement(139..3654)
                     /locus_tag="ACHUOY_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR0684326.1"
                     /translation="MDKNALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT          960 a          969 c          786 g         1070 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttg
//
LOCUS       DAZOYL010000321         3783 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZOYL010000321 DAZOYL010000000
VERSION     DAZOYL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.322"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..285
                     /locus_tag="ACHUOY_003675"
     CDS             <1..285
                     /locus_tag="ACHUOY_003675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684327.1"
                     /translation="THHIGRIMNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAK
                     LTGEKILRWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            282..707
                     /locus_tag="ACHUOY_003676"
     CDS             282..707
                     /locus_tag="ACHUOY_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR0684328.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            779..1843
                     /locus_tag="ACHUOY_003677"
     CDS             779..1843
                     /locus_tag="ACHUOY_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR0684329.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            1850..2596
                     /locus_tag="ACHUOY_003678"
     CDS             1850..2596
                     /locus_tag="ACHUOY_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0684330.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            2618..3343
                     /locus_tag="ACHUOY_003679"
     CDS             2618..3343
                     /locus_tag="ACHUOY_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR0684331.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            3359..3781
                     /locus_tag="ACHUOY_003680"
     CDS             3359..3781
                     /locus_tag="ACHUOY_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0684332.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
BASE COUNT         1022 a          857 c         1126 g          778 t
ORIGIN      
        1 acacatcaca tagggcggat tatgaatctt ggagaatatt tgcatcattt tcatataacc
       61 cagagttcat ttgctgagat tgttggcgca tcccaaggga tggtcagcca cgttataacg
      121 gggcgtgcaa aacttacagg agaaaaaata ttacgttggt gtgaagcaac gggatgggtg
      181 gtgaccccac atgagattga cagtaaaacc taccccaacc caactgacgg cttgcctgct
      241 gagtttcagg ctaacacaca accatcggcg ggggttgatt catgaaaatc aagcatgaac
      301 acatccgcat ggcgatgaaa gcctgggcgc atccggacgg tgaaaaagtt ccggcagctg
      361 aaataaccag ggtttatttc gaactgggaa tgacgtttcc ggttctgcat aacgacagca
      421 agcacaacac gctttatctc aacacccaaa agattttccg ttggctggat aaagacaccc
      481 ctgacgctgt tgaaaaaatt caggcgttgt tacctgcgat cgaaagggca atgccacctc
      541 cgctggtggc tcgaatgcgc agccacagtt cagcttattt tcgggaactg gtggagacgc
      601 atgaacgact ggtgagagac gctgatgatt ttgtcgcagt ggcaatcgcc ggtttcaatc
      661 agatgaaccg tggtggcccg gcaggaaatg ctgtggcagt acattgactg acaatagcca
      721 tatcgaatcg ctcccggcaa ctcgtgagta aaaagattcg gtatcagaag aggtgagtat
      781 ggctaacgcc tggctcagat tatggcatga catgccaaat gaccctaagt ggcgaacaat
      841 tgccagggtg tcagggcagc caattgcaac agtgatggca gtgtatatcc acctcctggt
      901 gagcgcgtca cgaaatgtca cgcgaggtca cattgatgtc acgacagaag atttggcaag
      961 tgcgctcgac gtgacagaag aggtaattga ttcaattttg cagacgatgc aggggcgggt
     1021 acttgatggt gatttaatca ctggatggga aaaacgccag gtgcttaaag aggacaacgg
     1081 caatatttcg caaaccgcaa aatctcctgc agagcgcaag agggcgcagc gagagaggga
     1141 aagaaagcgg gaacaaaatg gcgattgtca cggcgcgtca cgaaatgtca cgcacatgtc
     1201 acgacgagtc acgacagata cagataaaga tacagataaa gaagatcaaa acactatggt
     1261 ccatggcgta aaaaacgcca cgaaccaggc aggggatgtt cagaccgtca atcctggtca
     1321 gccagcaggc tcgacaccgg aagccgattc agcgtatgcg ctgaaagccg attcgggcgc
     1381 tgtgcagcag gtgatgaccg caaggccgga gcaatcactc caactgcagc agcctgaagc
     1441 cgattccgcc attcagcggg aagccgatcg ggtagccccg gaaaacaccg ggcagcctgt
     1501 gggacgagtg gattatccgg atgtgttcga acaggtctgg cgggaatacc cgttgcgtgc
     1561 cggggcaaac ccgaagaaat ccgcattcag tgcctggaag gccagattgc gcgagggggt
     1621 gccaccagag accatgctgg atggtgtgag gcgttacgcg agatacctgg cggcgaccgg
     1681 gaaagcggga acggaatttg ttcagcgagc gacgacgttt tttgggccgg accggaattt
     1741 tgaaaacccc tggttgctcc cggtaagcgg cacgaacaac cagcgttgtg tgaatcatat
     1801 ttctgaaccg gataccgaaa ttccgccggg attcaggggg tgatgtggca tgaaaaacat
     1861 tgcggcagcc ggggttcttg aacgtattca cagacttgca ccacaggggg cggttccacc
     1921 gtaccggacg gtggaggagt ggcgggaatg gcaacttgct gaaggacgaa aacgcagcga
     1981 ggagattaac cgccagaatc gccagttgcg ggtggaaaaa atcctgaatc gttcgggcat
     2041 ccagcctctg cacagcaaat gctcgtttgc gaattatcag gtgcagaacg acgggcaaaa
     2101 atacgcgctg agccaggcca aatccatagc tgacgaactg atgaccgggt gcacgaattt
     2161 tgtgttcagc ggtaaaaccg gcaccgggaa aaatcacctt gcagcggcga tgggtaaccg
     2221 gctgatggcg aaggggcgca gcgtgattat cgtcaccgag tctgatgtca tgagcgtgtt
     2281 gcatgacagc tacgacaacg gcaaatccgg tgaaaaattt ttacaggagc tttgcggggt
     2341 tgatttgctg gtcctggatg aaataggcat tcagcgggag acgaaaaacg agcaggtggt
     2401 attgcaccag ataattgatc gccggacagc atcactgtgc agtgtcggga tgttaacaaa
     2461 cctgaatcat gccgcaatga gcacgcttct tggtgagagg attatggacc gcatgaccat
     2521 gaacggttgt cggtgggtga cgtttaactg ggatagctgg cgttcaaatg tcagctttcc
     2581 gggagttgtg aagtaatttt tgttggagga tgttttaatg gaaactgtat ttgacgcact
     2641 gaaagcactg aaaagagcct cttcacatgt ggtggcgtcc cgccttggaa tcagccgtga
     2701 agatgcggtc aacgaactgt ggaaactgaa gcgccgtggt gaagcggata acaagggttc
     2761 gatgtggtgg ctgattcagg ctggtgaaag tgaaccagtg tcaccggtac cgaaagtgac
     2821 agcgcaaatg ctgactgagg cgattgaaca acatggccca caaacggcgg atgagctggc
     2881 actgatgttc gggattacct cccgccgggc gaattcatcg ctggccatgg caatcagcaa
     2941 agggcgtctg attcgcgtga atcagggcgg taaatttcgg tactgcatac cgggcgctga
     3001 tttaccggca gagccgaaag ccgcatccat agcggaaacg gatggtaaag tctttcctca
     3061 gccagcaggt gttgcgttac cggtacagga agctgcaaca caggaagaaa ttaaaacaga
     3121 aactgtggcg gacattgtgc agtcgttgcc atcgttcacc gaaacgcaag cggatgacct
     3181 ggttttacca tcgctgcata tggcaaaccg cgaactgcgt cgggcgaaaa gtcatgtcca
     3241 gaagtgggag cgtgtctgcg ccgcgttgcg ggagctgaac aagcaccggg atattgttcg
     3301 acagattgtc gattcctccg gtcgtattgt gtcggaaaag tgattgccgg gggcgcttat
     3361 ggcaaaagta tttacacagg aagagcggga aaaaattaag gggcaggttg ttgaactcgt
     3421 acgccagagt gggcgcgaga cgttacgaca actggaagct aaaaccgggg cgacaagata
     3481 tttaataagc gttctcgcca gagagctggt tgccagtggg gatgtataca actctggcta
     3541 cgggttattc ccgtctgaac aggctcgtaa ggactggcaa aacgcccgca aaaaactctc
     3601 gagggcaaag ctgaagaaac catctgtggt tgatccggac cttatctggt cattaccgga
     3661 cggagaaata cgccgctacg acagacatca gaacataatc tgtcgcgagt gccggaagag
     3721 tgaagttatg cagcgtgtac cggctttcta tcagggtgat tttcaggagg cggtactgtg
     3781 agt
//
LOCUS       DAZOYL010000322         3740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZOYL010000322 DAZOYL010000000
VERSION     DAZOYL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.323"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            567..1556
                     /gene="araF"
                     /locus_tag="ACHUOY_003681"
     CDS             567..1556
                     /gene="araF"
                     /locus_tag="ACHUOY_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR0684333.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1626..3140
                     /gene="araG"
                     /locus_tag="ACHUOY_003682"
     CDS             1626..3140
                     /gene="araG"
                     /locus_tag="ACHUOY_003682"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR0684334.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3155..>3740
                     /gene="araH"
                     /locus_tag="ACHUOY_003683"
     CDS             3155..>3740
                     /gene="araH"
                     /locus_tag="ACHUOY_003683"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0684335.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTT"
BASE COUNT          926 a          848 c          995 g          971 t
ORIGIN      
        1 ttaatgatag tgctttttgt atcttgttga tattaatcaa tgaatttgaa ttcatgatga
       61 agtgatgatt ttataaaacg ttttttcatt tttgcgagat ggctctcatt atacgtgttc
      121 tgttaattaa tgagcacagt gataattaat ggcgaaatga atgacgtgca ttttccacat
      181 ctttgagttg cggttattac accatttcaa aaactcaacg ccaggtaatg cggcctattg
      241 actggttaaa aagaagacat cccgcatggg taccaaagac aacaaggatt tccaggctaa
      301 tcttatggat taatctgctg tgcattcgac aatttgtctg acaaattggc tttcccttat
      361 gtcttttccc gctaaattta tgcacgttct cactgtaatt ctgcgatgtg atattgctct
      421 cctatggaga attaatttct cgctaaaact atgtcaacac ggtcacttat cttttagata
      481 aaaggtaatg ctttgttttc cgattaattt aacgaaagtc attcgttttt gccctacaca
      541 aaacgacact aaagctggag agaaccatgc acaaatttac taaagccctg gcagccattg
      601 gtctggcagc cgttatgtca caatccgcta tggcggagaa cctgaagctc ggttttctgg
      661 tgaagcaacc ggaagagccg tggttccaga ccgaatggaa gtttgccgat aaagccggga
      721 aggatttagg gtttgaggtt attaagattg ccgtgccgga tggcgaaaaa acattgaacg
      781 cgatcgacag cctggctgcc agtggcgcaa aaggtttcgt tatttgtact ccggacccca
      841 aactcggctc tgccatcgtc gcgaaagcgc gtggctacga tatgaaagtc attgccgtgg
      901 atgaccagtt tgttaacgcc aaaggtaagc caatggatac cgttccgctg gtgatgatgg
      961 cggcgactaa aattggcgaa cgtcagggtc aggaactgta taaagagatg cagaaacgtg
     1021 gctgggatgt caaagaaagc gcggtgatgg cgattaccgc caacgaactg gatacggcac
     1081 gtcgccgtac aacgggttct atggatgcgc tgaaagcggc cggattcccg gaaaaacaaa
     1141 tttatcaggt acctaccaaa tctaacgaca tcccgggggc atttgacgct gccaactcaa
     1201 tgctggttca acatccggaa gttaaacatt ggctgatcgt cggtatgaac gacagcaccg
     1261 tgctgggcgg cgtacgcgcg acggaaggtc agggctttaa agctgccgat atcatcggca
     1321 ttggcattaa cggtgtggat gcgttgagcg aactgtctaa agcacaggca accggcttct
     1381 acggttccct gctgccaagc ccggacgtac atggctataa atccagcgaa atgctttaca
     1441 actgggtagc aaaagacgtt gaaccgccaa aatttaccga agttaccgac gtggtgctga
     1501 tcacgcgtga caactttaaa gaagaactgg agaaaaaagg tttaggcggt aagtaatttg
     1561 ccggaaaaat tcccctctgc atgatgcaga gggggtgtga acgaccagtg attcacggag
     1621 acgttatgca acagtctacc ccgtatctct catttcgcgg catcggtaaa acgtttcccg
     1681 gcgttaaggc gctgacggat attagttttg actgctatgc cggtcaggtt catgcgttga
     1741 tgggtgaaaa tggcgcagga aaatcaactc tcttaaaaat cctcagcggc aactatgcgc
     1801 caaccacggg ttctgtagtg attaatgggc aggaaatgtc cttttccgac acgaccgcag
     1861 cacttaacgc gggtgtggcg attatttacc aggaactgca tctcgtgccg gaaatgaccg
     1921 tcgcggaaaa catctatctc ggccagctgc cgcataaagg cggcattgtg aatcgctcat
     1981 tgctgaatta tgaggcgggt ttacaactta aacatcttgg tatggatatt gacccggaca
     2041 cgccgctgaa atatctctcc attggtcagt ggcagatggt tgaaatcgcc aaggcgctgg
     2101 cgcgtaacgc taaaattatc gcctttgatg agccaaccag ctccctttct gcccgcgaaa
     2161 tcgacaatct tttccgcgtt attcgtgaac tgcgaaaaga ggggcgggtg atcttatacg
     2221 tttctcaccg tatggaagaa atatttgccc tcagcgatgc catcactgtc tttaaagatg
     2281 gacgttatgt caaaaccttt accgatatgc agcaggttga ccacgacgcg ctggtgcagg
     2341 cgatggtcgg gcgcgacatt ggcgatatct acggctggca acctcgtagt tatggcgagg
     2401 agcgcctgcg tcttgatgct gtgaaagcac caggcgtgcg tacgccaata agtctggcgg
     2461 ttcgcagtgg tgaaattgtt gggctgtttg gtctggtagg ggcggggcgt agcgaattaa
     2521 tgaaaggctt gtttggcggg acgcaaatca ccgccggtca ggtttatatc gaccaacagc
     2581 cgatcgatat tcgtaaaccg agccacgcca ttgccgcagg catgatgctc tgcccggaag
     2641 atcgcaaagc ggaaggcatt attcccgtgc actccgttcg cgacaatatc aacatcagtg
     2701 ccagacgcaa acatgtgctc ggcggttgtg taatcaacaa cggttgggaa gaaaacaatg
     2761 ccgatcagca cattcgttcg ctcaacatca aaacgccggg cgctgagcaa ctgatcatga
     2821 atctctcagg cggaaatcag caaaaagcca ttctgggccg ctggttatcg gaagagatga
     2881 aggtcatttt gctggatgaa cctacgcgca gcattgatgt tggtgctaag cacgaaatat
     2941 ataacgtaat ttatgcgctg gcggcgcagg gtgtggcggt gctgtttgcc tccagcgact
     3001 tacctgaagt cctcggcgtt gccgaccgga ttgtggtgat gcgggaaggt gaaatcgccg
     3061 gtgaattgtt acacgagcag gcagatgagc gtcaggcact gagccttgcg atgcctaaag
     3121 tcagccaggc agttgcctga gtaaggagag tatgatgtct tctgtttcta catcgggttc
     3181 tggcgcacct aagtcgtcat tcagcttcgg gcgtatctgg gatcagtacg gcatgctggt
     3241 ggtgtttgcg gtgctcttta tcgcctgtgc catttttgtc ccaaattttg ccaccttcat
     3301 taatatgaaa gggttgggcc tggcaatttc catgtcgggg atggtggcgt gtggcatgtt
     3361 gttctgcctt gcttccggtg actttgacct ttctgtcgcc tccgtaattg cctgtgcggg
     3421 tgtcaccacg gcggtggtta tcaacctgac tgaaagcctg tggattggcg tggcagcggg
     3481 gttgctgctt ggcattctct gtggcctggt caatggcttt gttatcgcca aactgaaaat
     3541 aaatgctctg atcacaacac tggcaacgat gcagattgtt cgaggtctgg cgtacatcat
     3601 ttcagacggt aaagcggtcg gtatcgaaga tgaaagcttc tttgcccttg gttacgctaa
     3661 ctggttcggt ctgcctgcgc caatctggct caccgtcgcg tgtctgatta tctttggttt
     3721 gttgctgaat aaaaccacct
//
LOCUS       DAZOYL010000323         3735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZOYL010000323 DAZOYL010000000
VERSION     DAZOYL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.324"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHUOY_003684"
     CDS             complement(<1..182)
                     /locus_tag="ACHUOY_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR0684336.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="ACHUOY_003685"
     CDS             426..683
                     /locus_tag="ACHUOY_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR0684337.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHUOY_003686"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHUOY_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR0684338.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHUOY_003687"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHUOY_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR0684339.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHUOY_003688"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHUOY_003688"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR0684340.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3735)
                     /locus_tag="ACHUOY_003689"
     CDS             complement(3308..>3735)
                     /locus_tag="ACHUOY_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0684341.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          953 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaatt
//
LOCUS       DAZOYL010000324         3734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZOYL010000324 DAZOYL010000000
VERSION     DAZOYL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.325"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..1898
                     /gene="tssM"
                     /locus_tag="ACHUOY_003690"
                     /pseudo
     CDS             1..1898
                     /gene="tssM"
                     /locus_tag="ACHUOY_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3358
                     /locus_tag="ACHUOY_003691"
                     /pseudo
     CDS             1909..3358
                     /locus_tag="ACHUOY_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            3285..>3734
                     /locus_tag="ACHUOY_003692"
     CDS             3285..>3734
                     /locus_tag="ACHUOY_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0684342.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTN"
BASE COUNT          989 a          976 c         1012 g          757 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaat
//
LOCUS       DAZOYL010000325         3730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZOYL010000325 DAZOYL010000000
VERSION     DAZOYL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.326"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            65..172
                     /locus_tag="ACHUOY_003693"
     CDS             65..172
                     /locus_tag="ACHUOY_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0684343.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(259..1938)
                     /gene="bcsG"
                     /locus_tag="ACHUOY_003694"
     CDS             complement(259..1938)
                     /gene="bcsG"
                     /locus_tag="ACHUOY_003694"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR0684344.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1935..2126)
                     /gene="bcsF"
                     /locus_tag="ACHUOY_003695"
     CDS             complement(1935..2126)
                     /gene="bcsF"
                     /locus_tag="ACHUOY_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR0684345.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2123..3694)
                     /gene="bcsE"
                     /locus_tag="ACHUOY_003696"
     CDS             complement(2123..3694)
                     /gene="bcsE"
                     /locus_tag="ACHUOY_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR0684346.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT          956 a          937 c          950 g          887 t
ORIGIN      
        1 aatcttgaac ccaaacaact caagaatagc cgcgaaacgt tggcattaca acacaggcgg
       61 ctatatgacg ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctccggtcat
      121 tgctggcatt cttgccagta tgatcgtgaa ctggctgaat aagcggaagt aacgtgtcat
      181 gcgggcgtca ggctgccgta atggcaattt gcgcccggat caggccgcag aggttaaact
      241 ctgcggcctt tttcgtgctt actgcgggta aggcacccag tcgccgccgt tcaggcgaac
      301 gtacggttta tcctggtatt gaataactac tgcatttgag ttctcggaga ccggtgctgt
      361 ttgtggcaac ccactggtga gttttttcca gtcaacattg tcttcggtga aaatcttgcc
      421 atcaagaacg cgaaccacca gatcggagat agctaggaag ctgctcggtt ggtcaatgac
      481 aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat ttcaccccaa cggggacgtc
      541 tgtgatagac gggctaggga tatcacgtag gccagatacc tgcattctgt cgcccttcag
      601 cgcgccgccg tgttccggca ccacgaccac catcacttta cgacccgatt tctccagttc
      661 agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc cgcgctttgt aatccgctgt
      721 tttgctgacc cccggataat ggttgccgtc atgcagtgga agcgtgttgt agaacgtggc
      781 actacggctg tttttatctt tttcggtaac gtccagccag cggttaagca cggcggtatc
      841 gtcataaacc ggcgaaccat caaagcccag caaaataacc ggcagatttg tttgatccat
      901 caattcagtc tgcatgccgc cattttcgcg aacttctttc aaaaaaccgc cgaactggcc
      961 gttatgtccc atcatcaggt gctgggtaaa gcccagtttc gaaaggttat caaacagata
     1021 gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa gtctgcccgc agctggcgcg
     1081 cagtaaacgg atcgccgccg ggccactgta ggaggtggcg gagttaaagt tcttgaactc
     1141 aatatcgaaa tgcgaccaca gtggatgcga catcaacccg gcggcttcta tatccgacca
     1201 ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc tgagcatcag cgggcagcga
     1261 agacgggaag gtcgatttac gtttcgcctc cgcgttatag aaattattca gccaggcgtt
     1321 aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata tcacccacta ccgatgcgcc
     1381 acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc gttgttacag tggtcgtcgg
     1441 ttgtccggct ggccacaagg agaaacttgg tcccgccagg gtaagtacgt tcagccatag
     1501 cagtatggca accacaaaaa cggtaatgcg aatccattgt gacaggaata accaggccac
     1561 taataaaaca aaaatggccc caatcatctg ccagttaata aagcgtgtga caaggtcgat
     1621 taaataatcg gtactgaacc ccgccacctg cgaaccctgg ctcattatgc tttccgggcc
     1681 aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc ggcagggcaa tccagtggcg
     1741 caagcgatgc aggctgtagc gcggaatggg catcagcaga aacgtggcaa acaccaaatt
     1801 gaggagcgga tggaagttaa gatatcccgc ccacaacagg ccgaacttaa ccagaaaata
     1861 gaagttccag ccggaaaggc cgcgccagta ttgccagagg gaagaaggca tggcggtatt
     1921 ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt ccgtgcggcg taacgtcccg
     1981 gccggtttaa cataacgagg tttagcaaag aataaacgta aggtgtcgcg aatgcgtcgc
     2041 aaaaagtgcc gcgccagata gcccagcggg aaaaatatca gtgcgcaaac gacaataatt
     2101 tcaatgatat cgctgatggt catcatgatg agcgctccac agcatcatct aacagtcgca
     2161 tgggttctgg tattcgtcgc cagtggcgac catcgtgctc ggcattgatg accggtttag
     2221 aactttgcgt taaaggcagc ggcatgcccc attgttctgg ggcaagcaag cgcatctgca
     2281 ccagctcggc actgatttga tcatcttcaa accagaccat acggtttgag aaaatgtcgc
     2341 cagtaggcaa tgggaaaata tgattcaacg cggtatccag atcgttaatc cgacagaatg
     2401 agagaaacag caccagccga ttaccgccaa tggtcatgat atcgccggta cggttagggc
     2461 gacacagcgt cagggcttgt tcaacgcgga tacccggtac cggacgtagg gcaaccagaa
     2521 cgcctttacc gtgggcaggt aatagagggt tattcatcat gttgttgacg gcattacaga
     2581 acacatccca cttctggaaa ccacgcagtt tgagcggctg ggtcattgac agcaaggtag
     2641 tgatatcttc cggcacatag cgactaaact tctgcccttg cacgctttcg atcatcgtca
     2701 gacaacggga gagtggcgca ttccacggaa taaccatatt tgcaccgcag gccaataaca
     2761 aacgttcatc ggtggcgcgc aggctagcgg tattttcccg cacgaggatt ttcatcgcac
     2821 taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag tggctcgatt tgcgcatttt
     2881 gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg ggcttcattg aacaggactt
     2941 cgttattgtt gaacagttgc cagtgttccg atagcggcgg cgcaccttcc agtacagcaa
     3001 cattactcag aatgcgtttt tcgtcgctgc gtggttggat ctccgcctct tcgctttgaa
     3061 ctaatgtcca gataccattt tgttgctgaa cgctaagctg ctgacgggcg ctgacccctt
     3121 tttcgttgca ccagaaggca atatccagca aatgttggtc accctgaaaa cgcaaactgg
     3181 caagaccaaa aagtgaacgg tattcctcaa gcaacaatga aaattgttta tcgttattat
     3241 ttccgggatt aattaccaaa agcgaacaat ggtttaacct gctccattta ttcattttat
     3301 ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca tgcgttattt gcgcaaacaa
     3361 gaataaaaag gtaattatgg ggatcaatag aacactgcaa atcacgggcc aaatagtata
     3421 gacctttttc atgatttagc attgaaaata attttatttt ttccggccct tgagaatcat
     3481 ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat gaccgcgacg tgtgcggttt
     3541 cagcctggga tgcaattgtt tgattcgcca gactgatagc atcttcatgg cgatcgacgt
     3601 taaaccacca gacgccgcct gctggcatat gtcgcagctc atcccataat gatgagatac
     3661 cgatagagaa tacagggtcc acaatgtccc tcatgtcacc attttgtctg attatcagtt
     3721 tactagcgaa
//
LOCUS       DAZOYL010000326         3699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZOYL010000326 DAZOYL010000000
VERSION     DAZOYL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.327"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHUOY_003697"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHUOY_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0684347.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHUOY_003698"
                     /gene_synonym="citB"
     CDS             complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHUOY_003698"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR0684348.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2049..>3699)
                     /gene="dpiB"
                     /locus_tag="ACHUOY_003699"
     CDS             complement(2049..>3699)
                     /gene="dpiB"
                     /locus_tag="ACHUOY_003699"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR0684349.1"
                     /translation="LNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTASFED
                     YLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGDRHS
                     IRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVIGVV
                     SIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIARVV
                     RQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPADFF
                     TEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQIKQ
                     YVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFADRQV
                     AGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLRSDE
                     GNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLYLIA
                     SYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          847 a          973 c          897 g          982 t
ORIGIN      
        1 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt
       61 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      121 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      181 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt
      241 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      301 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg
      361 atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt
      421 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      481 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa
      541 gtgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
      601 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      661 catgaaatgt tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg
      721 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
      781 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
      841 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
      901 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
      961 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1021 ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1081 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     1141 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1201 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     1261 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1321 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1381 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     1441 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1501 cagtggtgca gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1561 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggtagct
     1621 cgtccttagg ttcaccgcga gcataagcat taaacatctc atcaatttgc ttctggctgg
     1681 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     1741 gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1801 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1861 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1921 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccagattt cccgccagta
     1981 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2041 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2101 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2161 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2221 cgcgtactga ccccctgctc aaatattttg tctcttagag actctggaac gccgcagccc
     2281 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2341 ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgccaaca
     2401 atggcggcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2461 acgatgatca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2521 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2581 gactccccct gcaccatcgc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2641 agcgtcgaca tccaattgtg atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     2701 tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     2761 atggccccca gcaaatcatc acctgaacga atagcttccc ggttggcaat aacgctcaga
     2821 ccattaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     2881 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc
     2941 cccagcatct ttcttgcgtt acgattgatg gcggtaatgt aaccatgcgg atccaccgca
     3001 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3061 tgctttggct ccatgcccat catttgtcga cggatatgcg cggccaggaa ccacgacagc
     3121 aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3181 cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca
     3241 tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgcccc tttcccggta
     3301 atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca
     3361 attttctccg gattaagatg gtaaaggcgg atcgagtgcc ggtcgccaat caccacataa
     3421 tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc
     3481 gttttcaccg cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc
     3541 atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3601 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3661 agtctttgga aaaatgcaaa ctgtttattc tcgttaagc
//
LOCUS       DAZOYL010000327         3689 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZOYL010000327 DAZOYL010000000
VERSION     DAZOYL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.328"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            247..1116
                     /locus_tag="ACHUOY_003700"
     CDS             247..1116
                     /locus_tag="ACHUOY_003700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR0684350.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(1276..1869)
                     /gene="rclC"
                     /locus_tag="ACHUOY_003701"
     CDS             complement(1276..1869)
                     /gene="rclC"
                     /locus_tag="ACHUOY_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR0684351.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(1881..2117)
                     /gene="rclB"
                     /locus_tag="ACHUOY_003702"
     CDS             complement(1881..2117)
                     /gene="rclB"
                     /locus_tag="ACHUOY_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR0684352.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(2226..3551)
                     /gene="rclA"
                     /locus_tag="ACHUOY_003703"
     CDS             complement(2226..3551)
                     /gene="rclA"
                     /locus_tag="ACHUOY_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR0684353.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1054 a          796 c          777 g         1062 t
ORIGIN      
        1 cattctccat taattcttcg tgatatcgca cattttcgtt atgcccaagg ccctaagtgt
       61 atcaaaaaca gcaaggtcga aggcaaaagc actggtagcg tctgcttcgt tgtccccgaa
      121 aatagagagg tttcagtcct acattattaa tgaatttttt gcataagtga tatcaaaatc
      181 cacatactaa tttgaggtta tgttttaacg tagactcatt gttcatgcct aatggaggga
      241 ctgacagtgg aattttccgt attaagtaac aatctgaaaa tgccgatgat gggatttggt
      301 gtttttcagg ttaccgataa aaacgtgtgc aaacagtcag tgttgaacgc tatccgcacg
      361 ggttatcgac tcatcgacac tgcagcggta tacggtaatg aagatgccgt cggtgaagcc
      421 gttcgtgaag ctatatctga aggtttatgt acccgagatg agttatttat aacgtctaaa
      481 ttgtgggggc aggatatgtt gaatcaggat acggcagcag caggtattga agcatcatta
      541 aaaaaatcgg gactagagta cttcgacctc tatttattgc accaggctat gcgcgattat
      601 ttcagtgtgt ggcgtgcact cgaagatgcc tatgaagagg gcaaattaaa agcaattggg
      661 gtttccaatt tctatcctca tgttctggcg aacttttgtg aaacggtaag agttaaaccg
      721 atggtcaacc aggtcgagtt gcatccctat tttgcccaac cagaggcgct ggcaaccatg
      781 aagtattata acgtgcagcc tgaagcatgg gctccgttag gtggtggacg acataaaccc
      841 tttgaaaata atctgcttca gagtattgca gatgcccatc aaaaatcgat ttctcaagtc
      901 attctgcgtt ggaatattca acggggagtg gtcgttattc cgaaatcgac acatcaacag
      961 cgtatcgaag aaaattttgc tatctgggat ttctcactga cagagaaaga aatggcacaa
     1021 attagttcgc ttgatttggg ttatgttggg gagtcggtaa aacattttaa tcctgaattt
     1081 gttcgtggtt gtcttgctgt aaaaatacat gattgatatt aatcatatat tttacctgag
     1141 acgacaagaa tcttttaaca ggggagtgat attgatcttc actctgtcat atctccggta
     1201 atatggcgtc aggcttcatc atcagaaagg gggcctgacg cctgaaaaag tgaacaacag
     1261 acagtgttcg gattatcaat attcagtttt taacgttgaa ctggattcat tactgcgttg
     1321 tttaagaatt tcccgcgccg aatctgccat tatcatgaca gcacctgcca gcatcagagt
     1381 atctttcaat accaagcgac cagcaccgga taaataaggg aaaccatgat gagcgtcacc
     1441 caatgcgggt acccatgcct ccggggtggt gattaaaaat gagagtgtta ccagcggcgt
     1501 ggtatatgcc atcagtcctc ccaataaacc taaccagcga ttgacaggat tagccaaaac
     1561 cagcagagca ataatcacct ccacgacgcc aagaccgttg gaaaaaccat aggtattatt
     1621 ggccgattgc catgcccttg cttctggttt gtattcgcct tcgtgagtca gatactgttt
     1681 atagtcttcc gggtgttcat aaaagaacga cattagtgga ctgtttgcga cgaatggtgt
     1741 aatgctgtct gcctcgtaag ggacaaactt taataaccca atccacataa aaacaatcgc
     1801 aatactcaga cgaatcaatg acaggccaat tttatctccc cgacttaaca ggtgcaggta
     1861 tttttccatt gtgaacatcc ttatttatac aatactgctg ttgcggttga attattatca
     1921 attcgcatcg atgtgacttt ccaggatgaa gcattatatt ttctggctat atcagcaatg
     1981 gtatcctcca gaactgtcgg tgatgctgat actgacgaga cgctgatatg tttcagaatt
     2041 attttatcat ctgcattggt ggaaaattcc ataacgccag ataatagtgt tgcaaataaa
     2101 acagattttt taaacatata aaataccatc aatgaagtga ttaagaagtt atagagtaac
     2161 agagaattaa taagattctt ttctctgaga cgccagaata tttgttctgg cgtctgattt
     2221 tgagtttatt tgactaatga aaatagatca ttgagtgatt cgctcatcga cggatgagta
     2281 aatatctgat cgcgtaatat gctataaggc agcccggcat ccatcaccat tttcactata
     2341 ttgatcatct cgtgggagtc aacacacagc agtgatgccc ctaatatacg ttgggtttta
     2401 ttatcaacaa tcgcttttaa taccccacga gtatcattca tcactctggc acgcggaatt
     2461 gcagctacag gcaatgtcac cacctgaata tcagcaccac tctctctggc ctgttcttct
     2521 gtcataccaa ccctggacag tggcggtgtc atgaatacgg aataaggcac atttttccga
     2581 tcatcagtac tacgtctgcc ttctcccagc aactcatcac gcacaatgcg gtaatcatcc
     2641 agtgaaatat aagtaaattg cagcccgccg gtaacatctc ccatcgccca aatattgtct
     2701 gcggtggtat gtaattgctt gtcaacgaca attgccccgc gctcgtttac tgcgataccg
     2761 gcattttctg gatgtaacga agcggtagcc ggttgacgac cggaagctat taacagtgca
     2821 tccaccgcca gttgggcgtg ctcgctatgc acttgcactt gattttcatg gtgactgatt
     2881 cgctccacat gggcattgag gataatatcg acgccctgat cgcgtaaaat cgtcgcgata
     2941 ttatcagcaa tatcccgatc ttcccgaggc aaaaacagcg aagctgcttc taaaatggtt
     3001 accttgctgc caaaattagc gaacatagag gcgaactcaa cgccaatata tccgccgccc
     3061 aaaataccta aatgcccagg caattctttt agattaagta atccggtgct gtcatataca
     3121 ccaggcgtgg tggtaattcc aggaattggc ggaaccacgg tttgtgcacc ggtattaata
     3181 aaaattttct cgccatgaat ttccagattt ccctcaggcc gatgaacacg caggctatga
     3241 ttattgataa actccgcctg gccgtcgatc acgtcgatat tgggcatatc cgcaagatta
     3301 tgaaaattct tattacgtaa aaaattaacc acttcatttt tacgctgtat ggcacggaca
     3361 aaatctgtgt gctgctgtgc gtcatgaacc aatgttttgg ttgggatgca gccgatatta
     3421 atacaggtcc cgccatacat tgcatttgat tgttcgatga gagccactcg ccaacctgct
     3481 tttgccagcg tgacggctaa tgtttttcca gccttgccaa aaccaataat aactgcctga
     3541 tatttattca tgatgatttc ctgtcgaaac acgttcattt gacatcatga ctatagacgc
     3601 aaaagagcaa ataccatttc ttcacaacgc ttgatatttt gtcttatcgt ctggtatttt
     3661 ctttttctta atcagaatca caaaacaaa
//
LOCUS       DAZOYL010000328         3663 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZOYL010000328 DAZOYL010000000
VERSION     DAZOYL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.329"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..461)
                     /locus_tag="ACHUOY_003704"
     CDS             complement(<1..461)
                     /locus_tag="ACHUOY_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR0684354.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHV"
     gene            complement(508..1227)
                     /gene="btsR"
                     /locus_tag="ACHUOY_003705"
                     /gene_synonym="yehT"
     CDS             complement(508..1227)
                     /gene="btsR"
                     /locus_tag="ACHUOY_003705"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR0684355.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1224..2909)
                     /gene="btsS"
                     /locus_tag="ACHUOY_003706"
     CDS             complement(1224..2909)
                     /gene="btsS"
                     /locus_tag="ACHUOY_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR0684356.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3131..3196
                     /locus_tag="ACHUOY_003707"
                     /pseudo
     CDS             3131..3196
                     /locus_tag="ACHUOY_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3424..>3663
                     /locus_tag="ACHUOY_003708"
     CDS             3424..>3663
                     /locus_tag="ACHUOY_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0684357.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWF"
BASE COUNT          978 a          946 c          846 g          893 t
ORIGIN      
        1 ttcacatgct ggcgcgctgc cagtccacgc agaaaataac gtaaaaattg atcgccgcat
       61 tcgcggaagt ttttatgatc cggtgcgcgc atcatcgctg taatttccgg catcgaaacg
      121 cggaactgct gttcggtgag gatcgccaga atgtcatcgg ttttcagcga aaacgcgatg
      181 cgtaattttt tcagcacgat gttgttatta atgcgacgtt ccggctccag tgccggagca
      241 gactcatcct tgccgcgttt ttcataaatc aggccattga ggaatgacga caaaacaatg
      301 tccggacaac gctgaaaacc ctcttcgtct tctttacgta gccagacggc gatctgttcc
      361 gcggtggctt cgacattacc cagcgccaga atacgcacca ggtcattatt attggctttc
      421 aaaatgtagc gcacgctgcg cagaatatcg ttacttagca tgaggccttc aggtgttaat
      481 gaggcaaaaa gccattttag cagtctttta caggccaatc gcctctttta agcttttcag
      541 ataacggcgg ctgaccggca cggttaagcc attacgcaaa atcaactcgg cctggccgtt
      601 atcttccaga cgaatctcct gtaaatgcgc gaggttaacc agatactgac gatggcagcg
      661 cagtagtggt gtacgacttt ccagggtacg taatgtcaat tcggtaaagc cctctttccc
      721 ttcgtggctg gtaacgtaga caccgctcat ccgactgctg acaaatgcca catctttcat
      781 ttgcagcaaa taaatccgac tatgccccgt acaagggata aatttcagcg cctgttgatt
      841 ttccggtaac agcgaaacat cctgcttgct gcgctcctga cgcaatcgcg ccagcgtttt
      901 ctccagtcgc gcttcatcaa ttggcttcag cagataatca aaggcatgtt cttcaaaggc
      961 tttaatagcg tattcgtcaa acgcagtgag aaaaacaata tacgggcgat gttccgggtc
     1021 aagcatcccc accatttcca gaccactgat gcgcggcatc tggatatcga gaaacagcac
     1081 atccgggcgc agtttatgca ccgcgccgat cccttccacg gcgtttgaac actctccaac
     1141 gatttcaata tcgctctgct cctgcaaaaa tacacgcagg ttctcccgtg ctaacggttc
     1201 atcatcgaca attaagactt taatcatgcc tcgtccctcc atggtagtcg taacgttatt
     1261 cgggtgtaac tatcaggctc acaggcgacg cttattccat agtcatcgcc aaaccgttca
     1321 cgtaaacgct tatccaccag attcatcccc agcccactgg cattggttac cggttgatac
     1381 aaaccggcat tgtcttcgat ctccagcatc aaatgttgcc cctcacgtcg ggcgctgatt
     1441 gccactcgcc ctgtatccag cagttgtgat gtcccatgtt taatggcgtt ttccactatc
     1501 ggttgcaggg taaacgcggg caattgctgc tgggataatt cttgcggaat agcaatgttg
     1561 acctgcaacc gcgactggaa gcgcgccttt tcaatttgca gataagcgtt cacatgttca
     1621 atttcgtcgg cgagagtaac aaactccgaa ggccgcttta agtttttgcg gaaaaaagtg
     1681 gaaagatact gcaccagctg gctggcctgt tcgctgtcgc ggcggatcac cgctttaatg
     1741 gtgttaagcg cattaaacaa aaaatgagga ttcacctggg cgtgaagcag tttgatttct
     1801 gactgggtga gcatcgcttt ttgccgctca tattgcccgg caagaatctg cgccgaaagc
     1861 agttgcgcaa tcccctcgcc cagcgtgcgg tttattgaac tgaataaacg gtttttggct
     1921 tcatacaatt tgatggtgcc catcacgcgc tgattttcgc cacgcaacgg aattaccagc
     1981 gtcgacccca gtttgcattg cggatgcaaa gagcaacgat aaggtacttc gttgccatca
     2041 gcgtagacca cttcaccggt ttcaatcgct tttaaggtgt aaggcgaaga aatcggtttg
     2101 ccgggtaaat ggtggtcgtc accaattccg gtaaaggcca gcaatttctc tcgatcggta
     2161 atcgcgactg caccaatatc cagctcctga tacagcacct gagccacttt catgctgttc
     2221 acttcgttaa acccctgtcg caaaatgcct tccgtcgagg ctgccacttt cagcgcagtg
     2281 gcagaaaaag ccgaagtgta tttttcaaac atcgcgcgtt tatcgagcaa tatacgcata
     2341 aacagcgccg cgccgacggt attggtgacc atcattggcg cagcaatatt actcaccaga
     2401 cgcaccgcat cttcataagg tcgggcgatc gccaggatga tcagcatttg caccatttca
     2461 gcgacgaacg tgacggcacc ggcggtaatg gggttaaaga ctttatcagt gcgcccgcga
     2521 cggatcagga tgctgtgtac caggccgccg agcaatcctt caacgatggt tgagatcatg
     2581 caacttagcg cggtcatgcc ccccatcgaa tatcgatgta agccgccggt cagaccaacc
     2641 agcccaccga cgaccggacc gccgagtaag ccgcccatta ccgcgcctat cgcacgggta
     2701 ttggcaatag aatcgtcaat gtgcaaccca aaccaggtgc ccatgatgca gaagatggaa
     2761 aagacgatgt agcagagaaa tttatgcggc agacgaaccg tgacctgcat taacggtatg
     2821 aataatggcg ttttactcat taaccacgca atgactaaaa aaacgcacat ctgctgaagc
     2881 agcagcaaca ccagattaaa atcgtacata cccgcaaacc acacttccct ttaaaacgcg
     2941 taacatacat tgcctgcgtt taactttctt tgaactcttg cagaaaaatg agaattcgtg
     3001 agtacgatca ctcaaaatcg cctggcaaaa ataaaatcac cctatagatg cacaaaaaac
     3061 gggcaaaact acctggttcg caaaactgcg tctaaagtta aaccgggacc tcgcgagcaa
     3121 gggtgagacg atggcgcttt acacaattgg tgaagtggcg ttgctttgtg atattaaccc
     3181 tgtcacgtta cgcgcgtgac gcaacatttt taaaaatcga cgaagatcgt ccaggagcgc
     3241 cgtttttcaa gggttggata gatgattcgt ttaccatgtc gacgaacttc gacgaatttc
     3301 gccgataaaa agtgtcccat acatgcccca ttgatgcccc ataaacaata tcaggaattg
     3361 acactggtta tccatacaga taaaaataac acctgtacat aaccacagtt acaggagggt
     3421 gttatgcgtg ttgagatctg tatcgctaaa gaaaaaatca ctaaaatgcc aaacggtgct
     3481 gtggatgcgt taaaggaaga attaacccga cgcatcagta aacgttatga cgatgtagag
     3541 gtgatcgtaa aagccaccag caacgatggc ctttctgtta cacgcaccgc agataaggat
     3601 tctgcaaaaa catttgttca ggagactctg aaagatacct gggagtctgc tgacgagtgg
     3661 ttt
//
LOCUS       DAZOYL010000329         3660 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZOYL010000329 DAZOYL010000000
VERSION     DAZOYL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.330"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(55..99)
                     /locus_tag="ACHUOY_003709"
     CDS             complement(55..99)
                     /locus_tag="ACHUOY_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684358.1"
                     /translation="MKRNDKILHYEGLN"
     gene            218..1942
                     /gene="ilvI"
                     /locus_tag="ACHUOY_003710"
     CDS             218..1942
                     /gene="ilvI"
                     /locus_tag="ACHUOY_003710"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR0684359.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1945..2436
                     /gene="ilvN"
                     /locus_tag="ACHUOY_003711"
     CDS             1945..2436
                     /gene="ilvN"
                     /locus_tag="ACHUOY_003711"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0684360.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2448..2534
                     /locus_tag="ACHUOY_003712"
     CDS             2448..2534
                     /locus_tag="ACHUOY_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR0684361.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2616..3620
                     /gene="cra"
                     /locus_tag="ACHUOY_003713"
     CDS             2616..3620
                     /gene="cra"
                     /locus_tag="ACHUOY_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR0684362.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          861 a          867 c         1011 g          921 t
ORIGIN      
        1 taattaaaaa aatagagaaa ttgctgtaag ttgtgggatt cagccgattt attatcaatt
       61 taatccttcg taatggagga ttttatcgtt tcttttcacc tttcctcctg tttattctta
      121 ttaccctgcg tttatgtctc tggctgccaa ttgcttaagc aagatcggac ggttaatgtg
      181 ttttacacat tttttccgtc aaacagtgag gcaggccatg gagatgttgt ctggagccga
      241 gatggtcgtc cgatcgctta tcgatcaggg cgttaaacaa gtattcggtt atcccggagg
      301 cgcagtcctt gatatttacg atgcattgca taccgtgggt ggtatcgatc atgttttagt
      361 tcgtcatgag caggcggcag tgcatatggc cgatggtctg gcgcgcgcga ccggggaagt
      421 cggcgtcgtg ctggtaacgt cgggtccagg ggcgaccaat gcgattactg gcatcgccac
      481 cgcttatatg gattccattc cattagttgt cctttccggg caggtagcga cctcgttgat
      541 aggttacgat gcctttcagg agtgcgacat ggtggggatt tcgcgaccag tggttaaaca
      601 cagttttctg gttaagcaaa cggaagacat tccgcaggtg ctgaaaaagg ctttctggct
      661 ggcggcaagt ggtcgtcctg gaccagtagt cgttgattta ccgaaagata ttcttaatcc
      721 ggcgaacaaa ttaccctatg tctggccgga gtcggtcagt atgcgttctt acaatcccac
      781 tactaccgga cataaagggc aaattaagcg cgctctgcaa acgctggtag cggcaaaaaa
      841 accggttgtc tacgtaggcg gtggggcaat cacggcgggc tgccatcagc agttgaaaga
      901 aacggtggag gcgttgaatc tgcccgttgt ttcctcattg atggggctgg gagcgtttcc
      961 ggcaacgcat cgtcaggcac tgggcatgct gggaatgcac ggtacctacg aagccaatat
     1021 gacgatgcat aacgcggatg tgattttcgc cgtcggggta cgatttgatg accgaacgac
     1081 gaacaatctg gcaaagtact gcccaaatgc cactgttctg catatcgata ttgatcctac
     1141 ttccatttct aaaaccgtga ctgcggatat cccgattgtg ggggatgctc gccaggtcct
     1201 cgaacaaatg cttgaactct tgtcgcaaga atccgcccat caaccactgg atgagatccg
     1261 cgactggtgg cagcaaattg aacagtggcg cgctcgtcag tgcctgaaat atgacactca
     1321 cagtgaaaag attaaaccgc aggcggtgat cgagactctt tggcggttga cgaagggaga
     1381 cgcttacgtg acgtccgatg tcgggcagca ccagatgttt gctgcacttt attatccatt
     1441 cgacaaaccg cgtcgctgga tcaattccgg tggcctcggc acgatgggtt ttggtttacc
     1501 tgcggcactg ggcgtcaaaa tggcgttgcc agaagaaacc gtggtttgcg tcactggcga
     1561 cggcagtatt cagatgaaca tccaggaact gtctaccgcg ttgcaatacg agttgcccgt
     1621 actggtggtg aatctcaata accgctatct ggggatggtg aagcagtggc aggacatgat
     1681 ctattccggc cgtcattcac aatcttatat gcaatcgcta cccgatttcg tccgtctggc
     1741 ggaagcctat ggtcacgtcg ggatccagat ttctcatccg catgagctgg aaagcaaact
     1801 tagcgaggcg ctggaacagg tgcacaataa tcgcctggtg tttgttgatg ttaccgtcga
     1861 tggcagcgag cacgtctacc cgatgcagat tcgcgggggc ggaatggatg aaatgtggtt
     1921 aagcaaaacg gagagaacct gattatgcgc cggatattat cagtcttact cgaaaatgaa
     1981 tcaggcgcgt tatcccgcgt gattggcctt ttttcccagc gtggctacaa cattgaaagc
     2041 ctgaccgttg cgtcaaccga tgatccgaca ttatcgcgta tgaccatcca gaccgtgggc
     2101 gatgaaaaag tacttgagca gatcgaaaag caattacaca agctggtcga tgtcttgcgc
     2161 gtgagtgagt tggggcaggg cgcgcatgtt gagcgggaaa tcatgctggt gaaaattcag
     2221 gccagcggtt acgggcgtga cgaagtgaaa cgtaatacgg aaatattccg tgggcaaatt
     2281 atcgatgtca caccctcgct ttataccgtt caattagcag gcaccagcga taagcttgat
     2341 gcatttttag catcgattcg cgaggtggcg aaaattgtgg aagttgctcg ttctggtgtg
     2401 gtcggacttt cgcgcggcga taaaataatg cgttgagaat gatctgaatg cgcaatttgc
     2461 agcccaacat gtcacgttgg gctttttttg cgaaatcagt gggaacctgg aataaaagca
     2521 gttgccgcag ttaattttat gcgcttagat gttaatgaat ttaacccata ccagtacaat
     2581 ggctatggtt tttacatttt acgcaagggg caattgtgaa actggatgaa atcgctcggc
     2641 tggcgggagt gtcgcggacc actgcaagct atgttattaa cggcaaagcg aagcaatacc
     2701 gtgtgagcga caaaaccgtt gaaaaagtca tggctgtggt gcgtgagcac aattaccacc
     2761 cgaacgccgt ggcagctggg cttcgtgctg gacgcacacg ttctattggt cttgtgatcc
     2821 ccgatctgga gaacaccagc tatacccgca tcgctaacta tcttgaacgc caggcgcggc
     2881 aacggggtta tcaactgcta attgcctgct cagaagatca gccagacaac gaaatgcgct
     2941 gtattgagca ccttttacag cgtcaggtcg atgccattat tgtttcgacg tcgttgcctc
     3001 ctgagcatcc tttttatcaa cgctgggcta acgacccgtt cccgattgtc gcgctggacc
     3061 gcgccctcga tcgtgaacac ttcaccagcg tggttggtgc cgatcaggat gatgccgaaa
     3121 tgctggcgga agagttacgt aagtttcccg ccgagacggt gctttatctt ggcgcgctgc
     3181 cggagctttc tgtcagcttc ctgcgtgaac aaggtttccg tactgcctgg aaagatgatc
     3241 cgcgcgaagt gcatttcctg tatgccaaca gctatgagcg ggaggtggct gcccagttat
     3301 tcgaaaaatg gctggaaacg catccgatgc cgcaggcgct gttcacaacg tcgtttgcgt
     3361 tgttgcaagg ggtgatggat gtcacgctac gtcgcgatgg taaactgcct tctgacctgg
     3421 caattgccac ctttggcgat aacgaactgc tcgacttctt acagtgcccg gtgctggcag
     3481 tggctcaacg tcaccgcgat gtcgcagagc gtgtgctgga gattgtcctg gcaagcctgg
     3541 acgaaccgcg taagccaaaa cctggtttaa cgcgcattaa acgtaatctc tatcgccgcg
     3601 gtgtgctcag ccgtagctaa gccgcgaaca aaaatacgcg ccaggtgaat ttccctctgg
//
LOCUS       DAZOYL010000330         3634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZOYL010000330 DAZOYL010000000
VERSION     DAZOYL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.331"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..598)
                     /gene="ydcO"
                     /locus_tag="ACHUOY_003714"
     CDS             complement(<1..598)
                     /gene="ydcO"
                     /locus_tag="ACHUOY_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR0684363.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLP"
     gene            690..1226
                     /gene="sutR"
                     /locus_tag="ACHUOY_003715"
     CDS             690..1226
                     /gene="sutR"
                     /locus_tag="ACHUOY_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR0684364.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1299..3260
                     /gene="rlhA"
                     /locus_tag="ACHUOY_003716"
     CDS             1299..3260
                     /gene="rlhA"
                     /locus_tag="ACHUOY_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR0684365.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3352..3582)
                     /locus_tag="ACHUOY_003717"
     CDS             complement(3352..3582)
                     /locus_tag="ACHUOY_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR0684366.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT          924 a          931 c          918 g          861 t
ORIGIN      
        1 tggggagaac gggtttaaag acaacatcag ttgtgacaac gtcaccttgc gcgataacga
       61 tcacgatccc aataatcatc gcggcaatta ccgcatagcg cggcgcaacg gccttggttg
      121 ccagccatac cagcaacata cttccacaca acgtaaattg accgtccaga ctggcaaacg
      181 cctgtaaacc aaagcgtaat aaaatcccgg caagcattgc cgccgcaagc gagtgcggaa
      241 taatgcgcat cagacgagca aagagtcccg ttatgccgca gaggactatt aacgcgttgg
      301 tgacaataaa aacgccgatg gcttcgttaa gtgttagtcc ctgcaatccg gtgaccaaca
      361 aagccgcgcc aggcgttgac catgcggtga gaacaggtac gcgataccat aatgtcagag
      421 tcagcgtact gacgcccatt gccagcccca gcgccgtcat ccagccagag atttgtgcag
      481 tggtggctcc ggcgacaatc gctgcttgcc agattattgc cgctgaactg gcgtagccaa
      541 ttaataccgc cagaaacccc gccagtagcg tgggtggagg aatagaaaac agacgcatag
      601 tcacctcgtg cgttatagcg tacaggcacc gtagcatttg tccgttataa cgcacaagtg
      661 ataaacttcc gttttgccgg aggagtcgca tggaaaatct cgctcgcttt ttatccacta
      721 cacttaaaca actacgccag cagcgcggct ggagtctttc gcgactggca gaagcgacgg
      781 gcgtttctaa agcaatgtta gggcagattg agcgtaatga gtccagtccg acggtcgcga
      841 cattatggaa aattgccacc gggttgaacg taccattttc aacatttatt tctccgccgc
      901 agtccgctat gccttcagtt tatgatccac aacagcaggc gatggtgatc acttcgctgt
      961 ttccttatga tccacaactc tgctttgaac acttctcgat tcagatggca tctggtgcaa
     1021 taagcgaatc gacgccccat gagaaggggg taatcgaaca tgtcgtcgtc attgatggac
     1081 aacttgatct gtgcgttgat ggggaatggc agacccttaa ttgtggggaa ggcgttcgat
     1141 ttgctgcaga cgtcacgcat atctatcgta atggtgggga gcaaaccgta cattttcatt
     1201 ccctcatcca ttacccgcgc agttaagcgg gaaaactatt tcgcaacgtc gcgcttgtgg
     1261 ctaaaatagc cgccattttt cagctactgg ataagaatgt gaccgtatct tctcatcgac
     1321 ttgaactgtt aagcccggca cgcgatgccg ccattgcccg cgaagctatt ttgcacggtg
     1381 ccgatgctgt ttatatcggc ggccctggtt ttggtgcccg tcataatgcc agtaatagcc
     1441 tgaaagatat tgccgagctg gtgccgtttg cccatcgtta tggtgcaaaa attttcgtca
     1501 cgcttaacac cattttgcat gatgatgagc tggaacccgc gcaacggctg attactgacc
     1561 tctaccagac cggtgtcgat gcgctgattg ttcaggatat ggggattctg gaacttgata
     1621 ttccgccgat tgaactgcac gccagtacgc agtgcgacat tcgtacagtt gaaaaagcga
     1681 agttcctctc tgatgttggc ttcacgcaga ttgtgctggc gcgagagctg aatcttgatc
     1741 agatccgcgc gattcaccag gctacggacg cgaccattga attctttatt catggggcac
     1801 tgtgcgtggc ctattcgggt cagtgctaca tttctcatgc gcaaacaggg cgtagcgcca
     1861 accgtggcga ttgctcgcag gcgtgccgtt tgccatacac attgaaagac gatcaggggc
     1921 gggtggtttc ctatgaaaaa catctgctgt cgatgaaaga taacgatcag actgccaacc
     1981 tcggcgcgct gattgatgct ggcgtacgct ccttcaagat tgaagggcgt tacaaagata
     2041 tgagctacgt gaagaatatc accgcccatt atcgccagat gctggatgcc attattgaag
     2101 aacgtggcga tctgacgcgt gcttcatcag gtcgtactga acatttcttt gttccatcga
     2161 cggaaaagac tttccaccgt ggtagcacag attattttgt gaatgcccgt aaaggcgata
     2221 ttggcgcgtt cgattcgccg aaatttatcg gcctgccggt gggtgaagta ttgaaagtgg
     2281 cgaaagatca tctcaatgtt gccgtcaccg agccactggc aaatggtgat ggcctgaacg
     2341 tgatgattaa acgtgaagtc atcggttttc gtgccaatac ggtcgagaaa accggagaaa
     2401 atcagtaccg cgtctggccc aatgaaatgc cagcagattt gcacaaaatt cgtccacatc
     2461 acccactaaa ccgtaatctt gatcataact ggcagcaggc actgacaaaa acctccagcg
     2521 aacgtcgggt ggcggtagac attgaactgg gcggctggca ggaacaactg attctgaccc
     2581 tcaccagtga agagggtgtc agcatcacgc atacgctgga cgggcagttc gacgaagcca
     2641 ataacgccga aaaagcaatg aacaatctga aggatggtct ggcaaaactg gggcaaaccc
     2701 tctattacgc ccgcgatgtg caaattaatt tgccgggggc gctgtttgta ccaaacagtc
     2761 tgttaaacca gttccgccgt gaagctgctg acatgctgga tgctgcgcgt cttgccagtt
     2821 accagcgcgg cagccgtaaa ccggttgctg atcctgcgcc ggtttttccg caaacgcatc
     2881 tgagtttcct cgcgaacgta tacaaccaga aagcgcgtga attttatcat cgctatggtg
     2941 tgcagctgat tgacgcggcg tatgaggcac atgaagagaa gggcgaagtc ccggtgatga
     3001 tcaccaagca ttgtctgcgc tttgccttta atctgtgccc gaaacaggcg aaaggcaata
     3061 tcaaaagctg gaaggcgacg ccaatgcaac tggttaacgg cgatgaagta ttaacgctaa
     3121 agtttgattg ccgcccatgc gagatgcacg tcattggcaa aatcaaaaat cacatactga
     3181 aaatgccgtt accgggaagc gtagtggcat ccgtaagtcc ggatgagctg ctgaaaacat
     3241 tgcctaagcg aaaagggtaa aacgccagtt ttctggttac tcacaactca ttgaatctgc
     3301 atgatattgc ctgccgggta aggcgtcacg ccgcatccgg catcaaacga ctcaacgttg
     3361 tcccgcttct ggtttgcgcg atttttgcca gtaaaaatgc tcgcgcaaac cttccgccga
     3421 ctcttccgcc acagcacgca attcatcgct gtccgcttca tgacgcagct gatgatccac
     3481 attctttacc cacacaaatt catgtccttt gtgccctgcc atgagttgtc ctgagaacag
     3541 cgcacacgtt aataagacaa ccgataacgc cttcgtaaac atcctgccac ctttttctta
     3601 ccttttgccg ctatgatgcc gatcgtttct tgag
//
LOCUS       DAZOYL010000331         3631 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZOYL010000331 DAZOYL010000000
VERSION     DAZOYL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.332"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..660
                     /locus_tag="ACHUOY_003718"
     CDS             <1..660
                     /locus_tag="ACHUOY_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR0684367.1"
                     /translation="ARALIQGLKHDLLADDTALRALIPQRLIAFDDAVRSTLKEEEKL
                     VNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQIGGKERYFFGNI
                     LWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTLLFGMKASGLG
                     RLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAKQIARLAEQST
                     D"
     gene            759..1772
                     /locus_tag="ACHUOY_003719"
     CDS             759..1772
                     /locus_tag="ACHUOY_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0684368.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1769..2599)
                     /gene="amiD"
                     /locus_tag="ACHUOY_003720"
     CDS             complement(1769..2599)
                     /gene="amiD"
                     /locus_tag="ACHUOY_003720"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR0684369.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2596..2919)
                     /locus_tag="ACHUOY_003721"
     CDS             complement(2596..2919)
                     /locus_tag="ACHUOY_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR0684370.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3130..>3631)
                     /locus_tag="ACHUOY_003722"
     CDS             complement(3130..>3631)
                     /locus_tag="ACHUOY_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0684371.1"
                     /translation="THSLKFINCHFFNVDMIRVNFSKCLFHDCSMHGVKIKPWLPVKW
                     TKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFLEIIFSLKNKYCIKLLSA
                     QERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRYIPQSQDGEDFRSHLQYK
                     LPLSAH"
BASE COUNT          899 a          943 c          886 g          903 t
ORIGIN      
        1 gccagggcgt tgattcaggg gctgaaacac gatctgctgg cggatgatac cgcgctacgt
       61 gcactcatcc cacaacggct gatcgctttc gatgacgcgg tacgtagcac gttgaaagag
      121 gaggaaaaac tggtcaactc cagcgactgg ggctacgacg ctcaggcctt tgcccgctgg
      181 cgaccggagt acggttattt tgccaaacag gcggggttta ccgttaaaac gtccgccagc
      241 cttgctgctt tatggcaggt agtgaaccaa atcggcggta aagagcgtta tttctttggc
      301 aatattttgt ggcagacacg ggcgttgatg gaccgcgcga tcggtcataa gctggcgaaa
      361 ggccgcccgg agcgcgaata tttacagact ggcgatgcgg tggatagctg gaaagtgatt
      421 gtcgttgaac cggaaaaaca acttacgttg ttatttggca tgaaagcgtc ggggctggga
      481 cgactgtgtt ttagcctgga agataaaggc gactatcgta ctatcgatgt ccgcgctttc
      541 tggcatccgc acggtatgcc ggggctgttt tactggttat tgatgatccc cgcgcatctg
      601 tttatttttc gcggaatggc aaaacaaatc gccagactgg cagaacaaag cacagattaa
      661 tcgacaaaac ctgcgttatt ctttcatgtt tcccattgca tcatggggga aatcacggaa
      721 gaatgcgcac gtcattttct caacaacagt ggattgatat gaaggtactg gttaccggcg
      781 ccaccagcgg cttaggtcga aacgcggtag agtttttatg ccagaaaggc atcagcgtgc
      841 gagcgaccgg tcgcaacgag gcaatgggca aattgctgga aaaaatgggc gcagagtttg
      901 ttccggcgga tctgaccgag ctggtttcat cacaagctaa agtgatgctc gcgggcattg
      961 atacgctgtg gcactgctcc agctttacct caccctgggg gacacaacag gctttcgatc
     1021 tggctaacgt tcgcgccact cgccgcctgg gtgaatgggc tgtcgcctgg ggtgtgcgta
     1081 actttattca tatctcttcc ccctccctgt acttcgatta tcaccaccat cgcgatatta
     1141 aagaagattt tcgccctcac cgcttcgcca acgagtttgc ccgcagcaaa gcagccagcg
     1201 aagaagtgat caatatgctt tcgcaggcga atccacaaac gcgctttact attctgcgcc
     1261 cacaaagtct gttcggaccg cacgataaag tctttattcc ccgtctggcg catatgatgc
     1321 accactacgg cagtattctg ttaccgcatg gcggcagtgc gctggtcgat atgacctact
     1381 atgaaaatac cgtgcacgca atgtggctgg caagccagga agcctgcgat aagctacctt
     1441 ccgggcgtgt gtacaatatc accaacggcg agcatcgcac actgcgcagc atcgtgcaga
     1501 agctgatcga cgagttgaat attgactgtc gtattcgttc cgtcccctac ccgatgctgg
     1561 atatgatcgc ccgcagcatg gagcgtttag gccgcaagtc agcaaaagag ccgccgctga
     1621 cccactacgg cgtctccaag ttgaattttg actttacgct ggatattacg cgtgcgcagg
     1681 aagagttagg ttatcagccg atcatcaccc tagatgaagg tatcgagaaa actgccgcct
     1741 ggctacgcga ccacggaaaa ctgccgcgct aatcctgccc gtatttctcc agcaatgctt
     1801 cggcaatcgc ctgagtttct gcatccgctt cgccgttata taacgtcggg cggaaatgca
     1861 tctggaatgc cataatcacg cgccgctgct cgcgcggtgt catatcaggt ttaacgtcat
     1921 aaccgtagcg cgccaaaagc tccagcaatg acgcggtatc taccggtgtg tgcggcgcgc
     1981 gcccggcaag gtaaaagtta acccgctgcg catccggcca ggcaccaatc ccctgctgcg
     2041 ccagttgctg ccagggaaat aatggccccg gatcgtcttt gcgctgcggt gcgatatccg
     2101 catgtgccac tacgttttcc ggcttgatgt gataacgggc aataatatct ttcgccagtg
     2161 gaataagcgc ctgaatctgt gtcggttcaa acggggcaaa atatttcact ccggccgatt
     2221 tttgccagcc acggttttcc agctcaatgc caatagaggt gtcgttaagg cgcgttgccc
     2281 cgcgccaggc gctaatcccc gcatgccagg ccagttcttg ttccggcacc agttgccaga
     2341 tgcgcggttt accgttgtat cgcggtggta ccgcagggac cagataatgc gagctgacct
     2401 gcttatcggt cagtgtcgcc agcgagctat caaaatcatc tgcggtgtag tggatcacca
     2461 gcactttaat gcgcggatac gccgcctgcg cctggcgtcg ggtatcaagc tgatatccct
     2521 ctttctcgac aatgcctttt tcgcctgcac accctgccaa taacagagcg gcagcgacca
     2581 gccagaagac tcttctcatc gacgcgtttt caccgccgta ccgctgacgc taaccatcag
     2641 catactgccg ttttgcccga ccgtttcgta gtcgatatca ataccgacga cggcatcggc
     2701 ccccagcgcc cgcgcctggg agcctaattc ctcaaaggcg atctcccgtg ctttacgcag
     2761 ttctttttca tacgcaccgg aacgtccgcc aacgatatcg cggataccgg caaagaaatc
     2821 acggaaaata ttggcaccta aaatcgcttc gccggtcacc acaccgcaat attcaacgat
     2881 ggtctggcct tccagagttg gggttgtaga aaattgcatg atcgatctcc ttgtttgtta
     2941 agccttcgat ctctaaagca ttatcgaact gatatgccat gttgggaaga aggttttgat
     3001 cagtttagga aatcatctca ttgtgtcaaa aacacccact taagtcttta cgattatccg
     3061 ttgcataacc cgatattggt ttcattatag ggtaccgctt acctgtctta ttatgttgca
     3121 ccaacaacat taatgagcgg aaagaggcaa cttatattgt aaatgactcc taaagtcttc
     3181 cccatcttgt gactgaggta tatacctctg gattgctttt tggtcagacg ccgcaaaaca
     3241 agaaagtaat aagatctcaa tgcttttgtc attaatataa gcgaagatca ttcttgcaaa
     3301 ttcgtaaata aacgcccgtt cctgagcgct taatagttta atgcaatatt tattcttaag
     3361 agagaaaata atttcaagga aagcatccgc agcaaccttc acgttattcc cggcgctcat
     3421 attatatata tcccttgcac agatagagta cagagatagt aaacagccat acaaataatc
     3481 atttattaac tcttttgtcc attttactgg caaccatggt tttatcttga caccatgcat
     3541 actacagtca tgaaaaagac atttgctgaa attaactctt atcatatcaa cattaaaaaa
     3601 atggcaatta ataaatttta aagaatgagt a
//
LOCUS       DAZOYL010000332         3587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZOYL010000332 DAZOYL010000000
VERSION     DAZOYL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.333"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>3587
                     /gene="traI"
                     /locus_tag="ACHUOY_003723"
     CDS             <1..>3587
                     /gene="traI"
                     /locus_tag="ACHUOY_003723"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR0684372.1"
                     /translation="PVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVR
                     MAEWMQTLKETGFDIRAYRDAAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERK
                     VQFMYTDLLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDE
                     LSVRALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRE
                     RVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTV
                     IVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGG
                     EQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQ
                     GVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSH
                     SLTLRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQV
                     ASVSEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQM
                     AMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAI
                     SLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQ
                     IKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKL
                     TSGQRAATRMILETSDHFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTH
                     RAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYA
                     LIAVGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSL
                     INRDVERALSGLERVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAF
                     PDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQV
                     MVPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTR
                     LISPREAVAEGVTLYTPDTIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLS
                     DGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRK"
BASE COUNT          787 a          967 c         1195 g          638 t
ORIGIN      
        1 accggtggag gccttttccg gacgctcaca gactatccgg gaggccgtcg gggaagacgc
       61 ctcgctgaaa tcccgggatg tggcggcgct ggatacgcgt aaatccaaac agcacgtcga
      121 tcctgaagtc agaatggccg agtggatgca gacgctgaag gaaaccgggt tcgacatccg
      181 ggcatatcgt gacgcagcgg aacaacgggc atatacccgc acgcagacac ccggacccgc
      241 ttcacaggac gggccggatg tgcagcaggc ggtgacacag gccattgccg gattaagtga
      301 acgcaaagtg cagtttatgt atacggacct gctggccagg acggttggca tactgccgcc
      361 ggaaaatggt gtgattgaac gggcacgcgc cggtatcgat gaggccatca gccgtgagca
      421 gcttatcccc ctcgatcgtg agaaggggct gttcacgtcc gggattcata tgctcgatga
      481 gctgtcagtc cgggcactca gtcgtgacat catgaaacag aaccgggtga ccgtacatcc
      541 ggagaaaagt gtcccccgga cggccggtta cagcgatgcc gtcagtgttc tggcacagga
      601 ccgtccgtcg ctggccattg tgtccgggca gggcggtgca gccgggcagc gtgagcgggt
      661 ggctgaactg gtcatgatgg cccgggagca ggggcgggag gtgcagatta tcgctgctga
      721 ccgtcgctcg cagatgaacc tgaagcagga tgaacggctg tccggtgaac tgataaccgg
      781 acgtcgtcag ttgctggaag gcatggcctt cacgccgggc agtaccgtta tcgtcgacca
      841 gggcgaaaaa ctctccctga aagagacgtt aaccctgctg gatggtgccg cacgtcataa
      901 cgtacaggtc ctgataaccg acagcgggca gcgaaccggt acaggcagtg cactgatggc
      961 catgaaggat gccggggtga acacatatcg ctggcagggg ggagaacagc gaccggccac
     1021 catcatcagt gaaccggacc gtaatgtccg ctatgcccgg ctggccggag attttgcggc
     1081 cagcgtgaaa gccggagaag agagcgtggc acaggtcagc ggagtacggg aacaggccat
     1141 actgacacag gccattcgca gtgagctgaa aacacagggc gtgctcggac acccggaggt
     1201 aaccatgacc gccctgtcac cggtctggct ggacagccgg agccgttatc tgcgggatat
     1261 gtaccgcccg ggaatggtga tggagcagtg gaacccggag acacgcagtc atgaccgcta
     1321 tgtgacagaa cgtgtgacgg cgcagagtca cagcctgacc ctgcggaatg cgcagggcga
     1381 aacgcaggtg gtgcgtattt cttccctgga cagcagctgg tcgctgttcc ggccggaaaa
     1441 aatgccggtg gcagacggtg agcgtctgag ggtgacaggg aaaattcccg gactccgcgt
     1501 ctccggcggt gaccgcctgc aggtggcatc cgtcagtgaa gatgcgatga cggttgttgt
     1561 gccgggacgg gctgaaccgg ccaccctgcc tgtgagcgat tcaccgttca cggcactgaa
     1621 gctggagaac ggctgggtgg aaacgcccgg gcattccgtc agcgacagtg cgacggtttt
     1681 tgcctccgtc acacagatgg caatggacaa tgccaccctg aacggtctgg cccgcagtgg
     1741 tcgtgatgtc cggctgtatt cctcactgga tgaaacccgt actgcggaaa aacttgcccg
     1801 ccatccctcc tttacggtgg tttctgagca gataaaggcg cgtgccggtg agacattgct
     1861 ggaaaccgct atcagtctgc agaaagccgg gcttcacacg ccggcacagc aggccattca
     1921 tctggccctt cctgtgctgg aaagtaaaaa cctggccttc agcatggtgg acctgctgac
     1981 agaggcgaag tcgtttgctg cagaaggaac cggttttgct gacctgggag gggaaatcaa
     2041 tgcgcagata aaacggggtg atttactgta tgtggatgtg gcaaaaggct atggcacagg
     2101 cctgctggtt tcccgtgcgt cgtatgaggc agaaaagagc attcttcgcc atattctcga
     2161 aggtaaggag gcggtcacgc cgctgatgga gagagtacct ggcgaactca tggagaaact
     2221 gacatcagga cagcgcgccg ccacccgtat gatactggaa acgtccgacc atttcacggt
     2281 ggtgcagggc tatgccggtg tgggtaagac cacacagttc cgggcggtga tgtcagccgt
     2341 gaacatgctg ccggagagtg aacgtccccg tgtcgttggg ctgggtccca cgcaccgcgc
     2401 ggtcggggag atgcgcagcg ccggcgtgga tgcgcagaca ctggcgtcct ttctgcatga
     2461 cacgcagctg cagcagcgca gcggagaaac gccggatttc agcaacacgc tgttcctgct
     2521 cgatgagagc tcaatggtgg gcaataccga catggcacgg gcatacgccc tgattgcggt
     2581 cggtggcggt cgggctgtgg ccagcggtga cacggaccag ctgcaggcca tcgcgcctgg
     2641 tcagcctttc cgtctccagc agacgcgcag tgctgccgat gtggtcatca tgaaggagat
     2701 tgtgcgtcag acgccggaac tgcgggaggc ggtatacagc ctgattaacc gggatgtgga
     2761 aagggcgctg tccgggcttg agcgtgtgaa accgtctcag gtgccacgtc tggagggcgc
     2821 atgggcaccg gagcactccg tgacggagtt cagtcacagc caggaagcga aactggcaga
     2881 agcgcagcag aaggcgatgc tgaaaggcga ggcttttccg gatgtcccca tgacactgta
     2941 tgaagccatt gtccgcgact ataccggcag gacaccggaa gcacgggagc agacgctgat
     3001 tgtcacgcac ctgaatgagg atcggcgcgt actgaacagc atgattcatg atgcacggga
     3061 aaaggccgga gaactggggc aggtgcaggt catggtgcct gtcctgaaca cagcgaatat
     3121 acgcgacggg gagctgcgtc gtctctccac ctgggagaat aacccggatg cccttgccct
     3181 ggtggataat gtgtatcacc ggattgccgg tatcagcaag gatgacgggc tgataaccct
     3241 gcaggatgcg gaaggtaaca cgcggctgat ttcgccccgg gaggcggtgg ctgaaggcgt
     3301 cacgctgtac accccggaca ccatccgggt ggggaccggt gacaggatac gcttcacgaa
     3361 gagtgaccgg gagcgcggtt atgtggccaa cagcgtctgg acggtgacag cggtttccgg
     3421 tgacagtgtc acactttctg acggtcagca gacccgtgtg attcgccccg gtcaggagcg
     3481 ggcagagcaa catattgacc tggcctatgc catcaccgcc cacggtgcac agggggcgag
     3541 tgaaaccttt gccatcgcgc ttgaaggcac ggaaggtaac cggaaac
//
LOCUS       DAZOYL010000333         3577 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZOYL010000333 DAZOYL010000000
VERSION     DAZOYL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.334"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUOY_003724"
     CDS             <1..196
                     /locus_tag="ACHUOY_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684373.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="ACHUOY_003725"
     CDS             207..374
                     /locus_tag="ACHUOY_003725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684374.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            371..715
                     /locus_tag="ACHUOY_003726"
     CDS             371..715
                     /locus_tag="ACHUOY_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684375.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="ACHUOY_003727"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="ACHUOY_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0684376.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1383..1469
                     /locus_tag="ACHUOY_003728"
                     /pseudo
     CDS             1383..1469
                     /locus_tag="ACHUOY_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1466..1738
                     /locus_tag="ACHUOY_003729"
     CDS             1466..1738
                     /locus_tag="ACHUOY_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684377.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1740..2798
                     /locus_tag="ACHUOY_003730"
     CDS             1740..2798
                     /locus_tag="ACHUOY_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0684378.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2799..3179
                     /locus_tag="ACHUOY_003731"
     CDS             2799..3179
                     /locus_tag="ACHUOY_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0684379.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3176..>3577
                     /locus_tag="ACHUOY_003732"
     CDS             3176..>3577
                     /locus_tag="ACHUOY_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0684380.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          968 a          808 c          980 g          821 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc ttccagacta cacaattctg ataattcagc
     1501 cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt ggtaccagca
     1561 tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca ggcgcggatg
     1621 cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg tcaggctccc
     1681 ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct ggaggtaatg
     1741 tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc gttaagccag
     1801 gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg gaaccaaaaa
     1861 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg gtggaagaca
     1921 aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt ggtgctggtg
     1981 cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac ggcgattatc
     2041 atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     2101 gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct catcaaaacc
     2161 tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag gagcgtgaat
     2221 tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg
     2281 aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct gtgtacagtg
     2341 aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag cgcacaaaaa
     2401 atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa aagacggtgg
     2461 tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag aaatcacaac
     2521 gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg acgtgtggca
     2581 atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg ggaacaaagg
     2641 ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta catgccggag
     2701 taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt tttttaatgc
     2761 acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc atagaatttg
     2821 ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc acatattttg
     2881 tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc cagcagcggc
     2941 tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca ccggataagc
     3001 gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg catgcggggt
     3061 tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag ccagtatctg
     3121 gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc aaaaaatgaa
     3181 actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg atgcatcggc
     3241 ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
     3301 gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
     3361 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
     3421 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
     3481 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct gctgtcacgg
     3541 aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DAZOYL010000334         3568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZOYL010000334 DAZOYL010000000
VERSION     DAZOYL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.335"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..401)
                     /locus_tag="ACHUOY_003733"
     CDS             complement(<1..401)
                     /locus_tag="ACHUOY_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0684381.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
     gene            453..1058
                     /gene="wrbA"
                     /locus_tag="ACHUOY_003734"
     CDS             453..1058
                     /gene="wrbA"
                     /locus_tag="ACHUOY_003734"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0684382.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            1079..1306
                     /locus_tag="ACHUOY_003735"
     CDS             1079..1306
                     /locus_tag="ACHUOY_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR0684383.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(1344..2585)
                     /gene="agp"
                     /locus_tag="ACHUOY_003736"
     CDS             complement(1344..2585)
                     /gene="agp"
                     /locus_tag="ACHUOY_003736"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR0684384.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2879..3568)
                     /locus_tag="ACHUOY_003737"
                     /pseudo
     CDS             complement(2879..3568)
                     /locus_tag="ACHUOY_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          937 a          858 c          805 g          968 t
ORIGIN      
        1 aacttcacag ggtctgttaa ggctgacttg tcgtagagtt caccatcacc cagagtcagt
       61 atagagaccc ccttcctcag tatctgcctg acatcatcta tagctgtgtc gatatcagcc
      121 ctactgaagc ggtcaaagtt ctctacaaca atcaaggaac cttctggaat agccccagac
      181 ataacatttt caaacatacg tctgaagtca gaaccttgat tcttatgttg ctggtgaaaa
      241 ccagatttac ccttatcaac atatctgtga gcaggaagaa ggtaataatc gtttttgtgc
      301 ttagagtaga aagcatcaat gaggtttgtt tgccgtctta aggagtctcc tgagtcttgc
      361 tctttagtac tccacctgat gtatgtcact agcagcttca tatcttcgga ctatgcttat
      421 ctaagtggtt cattgaacag gagaacaatg atatgagtgt taaggcaaga atactagtgc
      481 tttattattc catgtacgga catattgaaa cgatggcacg cgcagtcgct gagggtgcaa
      541 gcaaagtcga tggcgcagaa gttgtcgtta agcgtgtacc ggaaaccatg ccgccgcaat
      601 tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg caagaactgg
      661 ccgattacga cgccattatt tttggtacac ctacccgctt tggcaacatg tccggtcaaa
      721 tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc cggcgcacta tacggaaaac
      781 tggcgagcgt ctttagttcc accggtactg gcggcggtca ggaacaaact attacttcaa
      841 cctggacgac ccttgcgcat cacggcatgg tgattgtccc cattggctac gcagcgcagg
      901 aattatttga cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc accatcgcag
      961 gcggtgacgg ctcacgccag ccaagccagg aagaactgtc tattgctcgt tatcaagggg
     1021 aatatgtcgc aggtctggca gttaaactta acggctaatc ttcaacagga ggatacgcat
     1081 gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc
     1141 gccggaaata gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa tggcagaaaa
     1201 gatttgggaa gaaggtagcg atgaagtgtt agtcaaagcc tttgctaagg ccgataaaga
     1261 ttcgcttttt tggggcgaac agaccatcga acgtaaaaac gtttaaccag cgactccccc
     1321 gcttctcgcg ggggagtttt ctgttatttc accgcttcat tcaacacgct atcaaattta
     1381 tccatcgggc agaaaccgtt ggcatctatc gggcaaccgc ttaattccag cgtcacacgc
     1441 tgagcaggtg cctgcagggt taacgcatcg gcattacgta actgttccgc actctgatac
     1501 acatattcaa ttttcatcaa atcgcgattg gctttgctgt catgccaacg ctggaaaacg
     1561 attttgccgc caatcggcgt gcgttcgttc tggtcatgca actgatacgg tttgaaatcc
     1621 agcgccgtta acagagaggc aatgttggag tcgtgcccaa ccaacactgt aattttcggt
     1681 gcgctggtgc gatcggtgac cagagctttg tcgatataac tgaccagcgg tttcgcaaca
     1741 ttgcgcgcca cttccggtga ggtaaacagg ctgtcctggt agccgttttt aagcttcgac
     1801 aacaccttcc actgctggtc agatttgatt tctccccagg ccacctgatc catcggaaaa
     1861 ccttcgtaat attgcaaagt aaacgcatct accagcgagt tgccgacttt cagcggcccg
     1921 gaaacacctg gttcttgttg atacttggcg ctaaaggtat ttttgccatc caccagcgaa
     1981 cactgttgtt tctctttaca ggcaggggaa tctttatagt taacgatttt ttccagtagc
     2041 tggtagctgt cggtaagctg gagtttgctg agctctttct ccattgccgc caccgcctgt
     2101 tcactgaatg cggcggaatc atcggtgatc accgggttaa aggttgggtc catagtgccc
     2161 attttttcct ggtgatgcac aggaatatca caccccggga atgcgccggt aataaagaac
     2221 tgtgcggtcg caacggtacg ttgcagacta ttggcatagg cataaacggt gtccggcggc
     2281 gggcattccc ccgatttcac catcccctgc tctgccagcc attcacgcat gtaatggccc
     2341 atatacactt cgagcacgcc acctttggtg gtgagttgcc caccggggac gtcccattct
     2401 ggccatttat tcggcgtcga ctgctccagc acactgccat tgttcgccag cggcgcacgt
     2461 aagttatggc ggctcatcat gagcacttgc tgtagctgat agccttccgg tacggtttgt
     2521 gcctgagcat ttgaagcgag taaaactatc cctgccacag ttgcggcgat tagcgttttg
     2581 ttcattcctg gcacctcttt tgttatcaat cattaaagag tgtgacagaa atatgacttt
     2641 tttccgggaa caggttcgca aaatggcttg ttgaacgcct gtttttctat atacatcttc
     2701 aaaaacaggc aaggtttgac gcaaactatg cctgattagc ccctgcctcg cgtacgccgc
     2761 tgataattgc acctgtggat aaacagaata tcagcggcgt tactaataca gaggcaatag
     2821 taccaataat aagagaagtg aggctaataa caatggtttt tagcacatgc ctttgtcatt
     2881 aaattgcata acgagttaaa aaatcaattt gcgcattcct ttccttattg tttaactcgt
     2941 aagcctgagc aaaaagttca ggagggaggt aaacgtcata atgattgcca gtgtttatta
     3001 aatacccata ctcaaggtcg tcatctgttg aaaaggattc attccctaag gagattgatg
     3061 ccacattgct gtaaacaata aataatgagc aaatgctatc tgaaagagca gtgaggaaat
     3121 ttttcacctc attaaccttc tgctctgggt cggtgtctaa aacaaggcgt gccttgttat
     3181 cattaaatat aaattcgggt aaatgcagta tgcatttttt gtcattcaag caactatcaa
     3241 taaattcttt tttaaaagac aaaaaactcc atgataaaaa accaccctgc ccctgaattt
     3301 tgttgtcaga ttgtagatta acgccaaata acgtctttac atcttccctg agattataga
     3361 atgccgtatc aacaagagcc tcgatcgttt gtttatcaac aattttaata aactcttcgt
     3421 atacttgctt ttgcgtctta tggccgccca gcgcccatga atactcatgt tcttttaaaa
     3481 ccagtatcta aatcagatat tcccggcgta tttatctgtt ctggctggat actcatattt
     3541 tcatcatcta aacaatactc agaaaaaa
//
LOCUS       DAZOYL010000335         3561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZOYL010000335 DAZOYL010000000
VERSION     DAZOYL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.336"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            129..1376
                     /locus_tag="ACHUOY_003738"
     CDS             129..1376
                     /locus_tag="ACHUOY_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR0684385.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHUOY_003739"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHUOY_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR0684386.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="ACHUOY_003740"
     CDS             complement(2228..2572)
                     /locus_tag="ACHUOY_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684387.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="ACHUOY_003741"
     CDS             2814..3416
                     /locus_tag="ACHUOY_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684388.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          886 a          715 c          817 g         1143 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct agggttgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttgaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggagagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgtc t
//
LOCUS       DAZOYL010000336         3544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZOYL010000336 DAZOYL010000000
VERSION     DAZOYL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.337"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHUOY_003742"
     CDS             complement(<1..140)
                     /locus_tag="ACHUOY_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0684389.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUOY_003743"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUOY_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR0684390.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2151)
                     /gene="xylB"
                     /locus_tag="ACHUOY_003744"
                     /pseudo
     CDS             complement(696..2151)
                     /gene="xylB"
                     /locus_tag="ACHUOY_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
     gene            complement(2223..>3544)
                     /gene="xylA"
                     /locus_tag="ACHUOY_003745"
     CDS             complement(2223..>3544)
                     /gene="xylA"
                     /locus_tag="ACHUOY_003745"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR0684391.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          924 a          983 c          783 g          854 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaagaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc ccccccccgt
      901 acggtaatcg agcttctgac cgctgatatc cgccagcatc tgacgccagt actcactacg
      961 cgccccgcca ccaatcaacg taacactttg cggtttaatg ccgcaggcat gcacgacatc
     1021 catgccatct gccagcgcat aacccacgcc ttccagcact gctcgcgcca gttcattggg
     1081 gccatgttga tgagtcaaac caaagaaaac ccccttcgcc tggggattat tgtgtggtgt
     1141 acgctcgccg gaaagataag gcagaaacca aactggctcg acactttcat cagcctgttg
     1201 agctgcagcg attaaagctg ggacattgca caggccggtt aatttcgcgg cccaatccag
     1261 acacgatgct gcactcagca ttacagacat taaatgccaa cgctgcggta gcgcatggca
     1321 aaagctgtgt acagcgcttt ctggcttgct taagaaccct tcgctgacag caaaatagac
     1381 ccccgacgtc cccagcgata acattgcctg attagcatca accattccca caccaactgc
     1441 accagctgca ttgtcgccac cgcctgcgac aactggcacc gtcgccatac cccacgcttt
     1501 cgcaacttca ggtaacaaag caccagtaat ttcgctgcct tcgtataatg cgggcatctg
     1561 gtcacgagat aagtcgcaag cctgcagcat gacgtcactc cagtcacgct ttgcgacatc
     1621 cagccacatg gtgccagctg cgtcagacat atcgctggca aactcccccg tcatacgcag
     1681 acgcaagtaa tctttcggta ataatacttt gtcgatttga cggaatatct ccggctcatg
     1741 ccgctgaacc catagcaatt taggcgcagt aaatccgggc atcatcaggt tgccggtaat
     1801 cactcgtgat tgcggaactc tcgcttccag caaagtgcac tcttgcgcac agcgcccgtc
     1861 gttccacaaa atggcagggc gcaatacccg ttgttgagca tccagtaagg ttgctccatg
     1921 catctggccg gcaataccca atgctttaac gtcctgcaga gaatgctgat cgcccagagc
     1981 tttcattgcg cgatcagttg cctgccacca ctgttccggg tcttgttccg accagagtgg
     2041 atgcgggcgc gaaacggtca gcttttccgt ttgcgaatca accacctcac cctgctcgtt
     2101 gagcaaaata acttttacgc ccgaggtgcc aagatctatc ccgatataca tatcgatcgt
     2161 tccttaaaaa aatgcccggt atcgctaccg ataaccgggc caacggactg cacagttagc
     2221 cgttatttgt cgaacagata atgatttacc agattttcca gttgctcctg gcgaccactc
     2281 tgatgcaccg gagacaaatt atgttcctga gcatatttgg ctaaatctgc cagtgacatt
     2341 tggcctttca ggatttgctg gcccaattcg ctattccagc cggaataacg ctgcgcgatg
     2401 cgtttatcca gctcgccatc ttcaatcatg cgcgctgcaa ttttcagcgc cagtgccatc
     2461 gtatccatcg cgccgatatg accgtaaaac agatcatatt tatcagtact ttgacgacgt
     2521 actttggcat cgaagttcag accaccggtg gtgaaaccgc ctgctttgag aatttcatac
     2581 atcaccagcg cattctcttc cacactgttc gggaactggt cggtgtccca gcccagttgc
     2641 gcatcgccac ggttggcgtc gacagaaccg aacaggccaa gcgcaatggc ggtggctatt
     2701 tcatgatgga aagagtgacc tgccagcgtc gcgtggttag cttcaatgtt cagtttaatc
     2761 tctttttcca gaccaaactg tttcaggaag ccatagaccg tcgcggcatc gtaatcatat
     2821 tgatgtttag tcggttcttg cggtttcggt tcgataagca acgtgccctg gaagccgatt
     2881 ttatgtttat gctcaaccac catctgcata aagcggccca gttgttcacg ctcctgacgc
     2941 aagtcggtat ttaacagcgt ttcgtaacct tcacgaccac cccacaggac atagttttca
     3001 ccgcccaatt tatgggttgc ttccatcgct gtaacaactt gcgttgccgc ccagctgaag
     3061 acttcaggat ctgggttcgt cgccgcaccc gcgccatagc gagggtttgt aaagcagtta
     3121 gcggttcccc acagcagctt cacgccgctc tcttcttgct tggctgccag gacatcaacc
     3181 atttgcgcaa aattattgat gtactctttt aacgacgcgc cctcagggga aacatccaca
     3241 tcgtggaagc aataaaatgg cacatgtaac ttgtggaaaa actcaaatgc gacatctgct
     3301 ttacgcttcg ccaacgccag tgcctcacca ggctgctgcc acggacgatt aaacgccccc
     3361 acaccaaaca tgtccgcccc gttccagcag aaggtgtgcc agtagcaggc ggcaaaacgc
     3421 aagtgctctt ccatacgctt acccaacacc agttcgtcgg gattgtagtg acggaatgct
     3481 aacgggtttg aggattttga gccttcataa cgaacgcgat cgagctggtc aaaataggct
     3541 tgca
//
LOCUS       DAZOYL010000337         3541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZOYL010000337 DAZOYL010000000
VERSION     DAZOYL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.338"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(33..1430)
                     /gene="ccp"
                     /locus_tag="ACHUOY_003746"
     CDS             complement(33..1430)
                     /gene="ccp"
                     /locus_tag="ACHUOY_003746"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR0684392.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1835..3484
                     /locus_tag="ACHUOY_003747"
     CDS             1835..3484
                     /locus_tag="ACHUOY_003747"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0684393.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          890 a          923 c          848 g          880 t
ORIGIN      
        1 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
       61 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      121 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      181 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      241 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      301 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      361 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      421 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      481 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      541 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
      601 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
      661 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
      721 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
      781 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
      841 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
      901 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
      961 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1021 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1081 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1141 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1201 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1261 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1321 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1381 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1441 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1501 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1561 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     1621 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     1681 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     1741 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     1801 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     1861 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     1921 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     1981 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2041 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2101 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2161 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2221 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2281 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2341 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2401 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2461 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2521 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     2581 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     2641 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     2701 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     2761 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     2821 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     2881 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     2941 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3001 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3061 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3121 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3181 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3241 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3301 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3361 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3421 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3481 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3541 g
//
LOCUS       DAZOYL010000338         3540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZOYL010000338 DAZOYL010000000
VERSION     DAZOYL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.339"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            186..2120
                     /gene="yeaG"
                     /locus_tag="ACHUOY_003748"
     CDS             186..2120
                     /gene="yeaG"
                     /locus_tag="ACHUOY_003748"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR0684394.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            2233..3516
                     /locus_tag="ACHUOY_003749"
     CDS             2233..3516
                     /locus_tag="ACHUOY_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR0684395.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT          956 a          869 c          889 g          826 t
ORIGIN      
        1 catttgccat ctacgcttaa ttttgaaggt gtatcaccgg gcacgttgtt ctcatcgtcg
       61 ataaaatggc atgagagttg ctgtgtttta gcaagagacg tcgttcagtt tacctcttcc
      121 gggagcctct actattcata tgaacggctc ttaacctgtg ctaaaaaacg aaaggacggc
      181 ataccatgaa tatattcgat cactatcgcc agcgatatga agctgccaag gacgaagagt
      241 tcacactgca ggagtttctt accacttgtc ggcaagatcg cagtgcttat gccaacgcgg
      301 ctgagcggct attgatggct atcggtgagc ctgtcatggt cgatacagcc caggaaccca
      361 gactttctcg actcttttct aaccgggtca ttgcacgtta tccggcattt gaagagtttt
      421 acggcatgga agacgcgatt gaacagattg tctcttatct gaaacacgcg gctcaggggc
      481 tggaagagaa gaaacaaatc ctgtatctgc tggggcctgt gggtgggggt aaatcatcgc
      541 ttgctgagcg actgaaatca ttaatgcagc tcgtaccgat ttatgtattg agcgccaacg
      601 gtgagcgtag cccggtcaac gatcatccgt tctgtctttt caatccgcag gaagatgcgc
      661 agattctgga aaaagagtat ggcattcctc gccgctatct cggcaccatc atgtcgccgt
      721 gggcggcaaa acgcctgcat gaatttggtg gcgatatcac taagttccgg gtagtgaagg
      781 tctggccgtc aattctgcaa caaattgcta tcgccaaaac agaacccggt gatgagaaca
      841 accaggacat ctccgcgctg gtagggaaag tcgatattcg taaactcgaa cactacgcgc
      901 agaatgaccc ggacgcctac ggctattccg gtgcgctgtg ccgcgccaat caggggatca
      961 tggaatttgt cgagatgttt aaagcaccga ttaaagtgct gcatccgctg ttaaccgcta
     1021 ctcaggaagg taactacaac gggacggaag gtatctccgc cctgccgttc aacgggatta
     1081 ttctcgccca ctcgaacgaa tccgaatggg tcactttccg taataacaaa aacaacgaag
     1141 ccttcctcga ccgtgtttac atcgtgaagg tgccgtattg cttacgcatt tccgaagaga
     1201 tcaaaatcta cgagaaattg cttaatcaca gtgaattgac tcacgcccca tgcgcccctg
     1261 gcacgctgga aacactgtca cgtttttcca ttctttcgcg cctgaaagag ccagaaaact
     1321 ccagcattta ttcaaagatg cgggtttatg atggcgaaag tctgaaagac accgatccca
     1381 aagccaagtc gtatcaggaa tatcgtgact acgccggtgt cgatgaaggg atgaaaggtc
     1441 tgtcgacgcg ttttgcgttt aagatcctct cccgcgtgtt caacttcgat catgtagaag
     1501 tggcggcaaa cccggtccat ctgttctacg tcctagaaca gcagatcgag cgcgagcagt
     1561 tcccgcaaga gcaggcagaa cgctatctgg agttcctgaa aggttatctg atcccgaaat
     1621 atgccgagtt tatcggcaaa gagatccaga cggcctacct tgaatcctat tccgaatatg
     1681 ggcaaaacat tttcgaccgt tatgttacct acgcggattt ctggattcag gatcaggagt
     1741 atcgcgatcc ggataccggg cagctgtttg accgcgagtc tcttaacgcc gagctggaga
     1801 aaatcgagaa accggcgggg atcagtaatc caaaagattt ccgcaacgag attgttaact
     1861 tcgtactgcg cgccagagcg aataacagcg gacgcaatcc gaactggacc agctatgaaa
     1921 aactgcgcac ggttatcgag aagaaaatgt tctccaatac cgaggagctg ttgccggtta
     1981 tctcgtttaa cgccaaaacg tcaaccgacg agcagaagaa acacgacgac tttgtcgacc
     2041 gtatgatgga aaaaggctac acccgtaaac aggtgcgttt actgtgcgaa tggtatttgc
     2101 gcgtacgtaa atcgtcttaa caaccctggc ccggtacaca tgctaccggg cctataacga
     2161 cagcgaaccg tgggccagag aaatggcaac acaggcgtag catacagttg gcaaatgtag
     2221 tacggggggc atatgaccgg gtttattgac cggcgtctga acggcaaaaa caaaagcatg
     2281 gtgaatcgcc agcgtttttt acgccgttat aaagcgcaaa ttaaacagtc gatctccgag
     2341 gccattaata agcgttcggt gactgacgtc gacagcggcg aatccgtatc cattcccacg
     2401 gaagatatta gcgaaccgat gtttcatcag gggcgtggcg gcctgcgcca ccgcgtacat
     2461 ccgggcaatg accatttcgt ccagaacgac cgaattgaac gtccccaggg tggcggcgga
     2521 ggttccggca gtggtcaggg ccaggccagc caggatggtg aaggtcagga tgaatttgtc
     2581 tttcagattt cgaaagatga gtatcttgat ctgctctttg aagatttggc cttaccgaat
     2641 ctgaaacaaa accaacaacg ccagctgacc gaatataaaa cgcatcgggc gggttatacc
     2701 gctaacggcg ttccagccaa tatcagcgtt gtgcgttcct tgcagaactc actggcgcga
     2761 cgcacagcca tgacggcggg caagcgacgg gaacttcatg cgctggaaga gaatttggcc
     2821 atcatcagca acagtgaacc tgcgcaactg ctggaagagg aacgtctgcg caaagaaatt
     2881 gcagaattac gtgccaaaat tgaacgcgtc ccttttattg acaccttcga tttacgttac
     2941 aagaactacg agaagcggcc cgatccctcc agccaggcag tgatgttttg cctgatggac
     3001 gtttccggtt caatggatca atccactaaa gatatggcta agcgttttta tattctgctg
     3061 tatctgttcc tcagcagaac gtataagaac gtggaagtcg tatacatccg ccatcatacc
     3121 caggcgaaag aagtcgatga acatgagttt ttctactcgc aggaaacagg cggcaccatt
     3181 gtttccagcg ccctgaaact gatggatgag gtagtgaaag agcgttataa cccggcacag
     3241 tggaatattt acgctgcaca agcatcggac ggcgataact gggccgatga ctctccgctt
     3301 tgccatgaaa tcctggcgaa aaaattatta cctgttgttc gttattacag ctatatcgaa
     3361 attacccgtc gtgcacatca gacattgtgg cgagaatatg agcatctgca atctactttc
     3421 gacaactttg cgatgcagca catccgcgac caggatgata tttatccggt gttccgtgaa
     3481 ctgtttcata aacaaaatgc aacagctaaa gactaaaact atcagccagg tcattatcgc
//
LOCUS       DAZOYL010000339         3418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZOYL010000339 DAZOYL010000000
VERSION     DAZOYL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.340"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..669
                     /locus_tag="ACHUOY_003750"
     CDS             1..669
                     /locus_tag="ACHUOY_003750"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0684396.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTRVQGTVVMFSA
                     SGKK"
     gene            complement(614..751)
                     /locus_tag="ACHUOY_003751"
     CDS             complement(614..751)
                     /locus_tag="ACHUOY_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR0684397.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(724..822)
                     /locus_tag="ACHUOY_003752"
                     /pseudo
     CDS             complement(724..822)
                     /locus_tag="ACHUOY_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(811..1050)
                     /locus_tag="ACHUOY_003753"
                     /pseudo
     CDS             complement(811..1050)
                     /locus_tag="ACHUOY_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1149..2039
                     /locus_tag="ACHUOY_003754"
                     /pseudo
     CDS             1149..2039
                     /locus_tag="ACHUOY_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            2042..2575
                     /locus_tag="ACHUOY_003755"
     CDS             2042..2575
                     /locus_tag="ACHUOY_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0684398.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2604..3131)
                     /locus_tag="ACHUOY_003756"
     CDS             complement(2604..3131)
                     /locus_tag="ACHUOY_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0684399.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(3133..>3418)
                     /locus_tag="ACHUOY_003757"
     CDS             complement(3133..>3418)
                     /locus_tag="ACHUOY_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0684400.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          970 a          755 c          744 g          949 t
ORIGIN      
        1 atggtgaaat caatgaaaac aaacacagat gatggggcta aaatttacac accacttacc
       61 ctaaagcttt atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct
      121 acaaaggaac accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt
      181 ggtgttggaa ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg
      241 gatttgaacg aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa
      301 cataaaatta gccatgatgt ttttgaacct tatcccgcgg cgttacatgg tcaatttgat
      361 tccatttcca tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt
      421 gtaatacgca atgcggcgca ggccttaact gacgatggaa ctctatacgg agccacaatt
      481 cttggcgatg gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag
      541 aaaggcatct tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag
      601 catttcgaga atgttaaaac cagggttcaa ggtactgtag taatgttttc cgcttcaggg
      661 aaaaaatagc atccaaccgc agcacgttct tgcttaagac gtgctgcggc ataatcccaa
      721 tgattactcc ctgacagggt tcgtaggcca ctcaatatca ggtgcagttg atgtatcaac
      781 acggttcagc aacacccgat actttttcta ggcttccacc accagcacga caagatgccg
      841 catacagtgc cccagtcagt ccagttttca gacaaccagt gcgtcacctt tttgaaggcg
      901 ctttaaagca cgttttaatc caggtcggcc tgtccttgtt ccgcttaatt tatcttcaaa
      961 tatttgttca tatcctgcac aaacaagagc gtttcgttgc aggtctgtat tctggtcatt
     1021 tgttgatacc cttacatagc caatcagcac gctgaatctc ccgtccaaaa gcacaaatca
     1081 tgccatgcag gccagaaacc gccattatct aaaacctcgg tttacgagaa ctcggcacaa
     1141 gcggggagaa aataccgtta ctcagtacag cgaatacctg gactaatcga caaacattca
     1201 gcggtggcct ttctggtgaa ctatccggca atgcttctac agctgcaaaa ttaaaaactg
     1261 ccaggaaaat aagcaatgtg gcttttgatg gttcctccga tatcacatta aaagcaagtc
     1321 atgttggtgc gtttgcctta gggaaaacag gaagcaccgt tgcgaatgat aaagcagttg
     1381 gatggaactg gagtagcgga gcctataacg caactattag tggtgcatca acgttaatta
     1441 ttcattttta tatgggagaa ggaagttgtc ctgcagctca gtttcggatt aattataaaa
     1501 atggcggtat tttttatcgt tcagcccgtg atggttatgg ttttgaagcc gactggtccg
     1561 aattttacac caccagaaaa ccttcagcag gagatgttgg tgcactgccg ttatctggtg
     1621 gtcaactgaa tggtgcactg ggtatcggaa catccagtgc tcttggcggt aattcgattg
     1681 tattgggtga taatgacacg ggctttaaac aaaaaggcga tggtaatctg gatgtttatg
     1741 ctaataacgt ccatgttatg cgctttgtct ccggaagcat tcaaagtaat aaaaccataa
     1801 atattacggg acgtgttaat ccctcggatt acggtaactt tgattcccgc tatgtgagag
     1861 atgtcagact tggcacacga gttgtttaga ccatgaagaa aggcgtgatg tatgaaaaat
     1921 caggccatgt aatcacgggg cttggtatta tcggtgaagt cgatggtgat gatccggcag
     1981 tattcagacc aatacaaaag ttaattaacg gaacatggta taacgtatcg caggtataat
     2041 catgcagcat ttaaaaaaca ttgtcgcagg taatccaaaa accgttgaac aataccagct
     2101 aacaaagaat tttgatgtta tctggttatg gtccgaagat ggaaaaaatt ggcatgagga
     2161 attaaaaaac tttcaggagg atacaataaa actggcttac acagttgagg gaataattat
     2221 tgctatagac aaggatgtat cagcaattaa tccagaaggt ttaagtgtcg ttgagttgcc
     2281 tgatattaca gcaaatcgcc gggctgatat ttcgggcaac tggatgttca aagatggtgc
     2341 ggtgataaag cgaacttata ccgaggaaga gcaaaggcaa caagcggaaa atgaaaagca
     2401 gagcctgctg cagctcgtca gggataaaac ccagctgtgg gactctcagc tacagctggg
     2461 catcatttcc gatgagaata aacaaaaatt aaccgagtgg atgctctatg cgcagaaggt
     2521 tgaatccaca gacacctcca gcctgccagt aacgtttccc gaacaaccag aatgagagaa
     2581 ggcccgctat cgggccttaa tttttattca ggcttttgtg gccattcagg atttgccgta
     2641 tccacacggc tgaccagaac actgtagcgt tcccatgctt ccagtcgtgt gcgttcctcg
     2701 tctgttgcca tattcagcct gacagctcgt tccagcggct ggatgactga ttcagcttcg
     2761 gaaagcaatg cggccttttg tgattcggcc tgttgttgca gttcctgttc tgtatattcc
     2821 ctctttacta ctttaccatc cagaaacatc cattttccgt tattgtctgc gtgacgattt
     2881 gccgtaatat caggaacttc aactatgctt agcccttcag gattaatagt tgaaacgtct
     2941 ttactgacag caacgatgat gttgttctca tcgtaagcaa tttttatggt gtctggctga
     3001 aaattctttt gttcctcata ccagtttttg ccatcttcgc aaaagagcca taccacgccc
     3061 gcttttttag ttagctggta ttgttccggt gttttcggat tcccggcctt aatatttttc
     3121 aggtgcatca tcttaaacac tccccacgtt gtaccatatt cccccaatca atttctgaat
     3181 tggccttctg gcaacagtgt cgacaatatc atccgcattg ctgttaattg cggctgttaa
     3241 aacataacct gacgcatcgc caaagccgta gtcgcgccat acgtttttat attcaatgct
     3301 gccaagacga atatcccgaa cgacatgatt atgcagccat gtgcttaacc agttgttttc
     3361 ccacactgaa ccataaatat cgccactaga cgcaacttgc gcaccacttc ccagcgca
//
LOCUS       DAZOYL010000340         3400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZOYL010000340 DAZOYL010000000
VERSION     DAZOYL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.341"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            134..1099
                     /gene="dusB"
                     /locus_tag="ACHUOY_003758"
     CDS             134..1099
                     /gene="dusB"
                     /locus_tag="ACHUOY_003758"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR0684401.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1125..1421
                     /gene="fis"
                     /locus_tag="ACHUOY_003759"
     CDS             1125..1421
                     /gene="fis"
                     /locus_tag="ACHUOY_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR0684402.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1507..2391
                     /gene="yhdJ"
                     /locus_tag="ACHUOY_003760"
     CDS             1507..2391
                     /gene="yhdJ"
                     /locus_tag="ACHUOY_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0684403.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2475..2654
                     /locus_tag="ACHUOY_003761"
     CDS             2475..2654
                     /locus_tag="ACHUOY_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR0684404.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(2657..3319)
                     /gene="acrS"
                     /locus_tag="ACHUOY_003762"
     CDS             complement(2657..3319)
                     /gene="acrS"
                     /locus_tag="ACHUOY_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR0684405.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          930 a          787 c          802 g          881 t
ORIGIN      
        1 tctcaccacg ctcattttaa atgcaattct ttgatccatc tcagaggatt ggtcaaagtt
       61 tggcctttca tctcgtgcaa aaaatgcgta atatacgccg ccttgcagtc acagtatggt
      121 catttcttaa ctcatgcgca tcggacaata tcagctcaga aatcgcctga tcgcagcgcc
      181 catggctggc attacagaca gaccttttcg gacgttgtgc tacgagatgg gagccggatt
      241 gacagtatcc gagatgatgt cttctaaccc acaggtttgg gaaagcgaca aatctcgttt
      301 acggatggtg cacattgatg aacccggtat tcgcaccgtg caaattgctg gtagcgatcc
      361 gaaagaaatg gcagatgcag cacgtattaa cgtggaaagc ggtgcccaga ttattgatat
      421 caatatgggt tgcccggcta aaaaagtgaa tcgcaagctc gcaggttcag ccctcttgca
      481 gtacccggat gtcgttaaat cgatccttac cgaggtcgtc aatgcagtgg acgttcctgt
      541 taccctgaag attcgcaccg gctgggcacc ggaacaccgt aactgcgaag agattgccca
      601 actggctgaa gactgtggca ttcaggctct gaccattcat ggccgtacac gcgcctgttt
      661 gttcaatgga gaagctgagt acgacagtat tcgggcagtt aagcagaaag tttccattcc
      721 ggttatcgcg aatggcgaca ttactgaccc gcttaaagcc agagctgtgc tcgactatac
      781 aggggcggat gccctgatga taggccgcgc agctcaggga agaccctgga tctttcggga
      841 aatccagcat tatctggaca ctggggagtt gctgcccccg ctgcctttgg cagaggttaa
      901 gcgcttgctt tgcgcgcacg ttcgggaact gcatgacttt tatggtccgg caaaagggta
      961 ccgaattgca cgtaaacacg tttcctggta tctccaggaa cacgctccaa atgaccagtt
     1021 tcggcgcaca ttcaacgcca ttgaggatgc cagcgaacag ctggaggcgt tggaggcata
     1081 cttcgaaaat tttgcgtaaa cagaaataaa gagctgacag aactatgttc gaacaacgcg
     1141 taaattctga cgtactgacc gtttctaccg ttaactctca ggatcaggta acccaaaaac
     1201 ccctgcgtga ctcggttaaa caggcactga agaactattt tgctcaactg aatggtcagg
     1261 atgtgaatga cctctatgag ctggtactgg ctgaagtaga acagcccctg ttggacatgg
     1321 tgatgcaata cacccgtggt aaccagaccc gtgctgcgct gatgatgggc atcaaccgtg
     1381 gtacgctgcg taaaaaattg aaaaaatacg gcatgaacta attcaggtta gctaaatgct
     1441 tgattaaaaa ggcgctactc ggcatgggga agcgcctttt ttataggtgt cacaaaggga
     1501 gtgaccatga gaacaggatg tgaaccgacc cggtttggta atgaagctaa gaccattatt
     1561 cacggtgatg cccttgccga acttaaaaag ctacctactg aaagcgtcga tctgatcttt
     1621 gccgacccac cgtataacat cggtaaaaat tttgatggtc tgatcgaagc ctggaaagaa
     1681 gatctgttta tcgactggct gtttgaagtg attgtagagt gccaccgcgt tctgaaaaag
     1741 cagggcagca tgtacattat gaacagtacg gaaaacatgc cctttatcga tctccagtgc
     1801 cgcaagcttt ttaccatcaa aagtcgcatc gtctggtcat atgacagttc tggagtacag
     1861 gcgaaaaaac actacggctc catgtacgaa cccatcctga tgatggtgaa agacgcaaag
     1921 aactacacat tcaacggtga tgctattctg gtagaagcca aaaccggatc gcagcgcgcg
     1981 ttgatcgatt atcgcaaaaa tcctccacag ccatacaatc atcaaaaagt accgggtaac
     2041 gtctgggatt ttccgcgcgt gcgttatttg atggatgaat atgagaacca cccgacgcaa
     2101 aaaccggaag ccttactgaa acgcattatt ctcgcctctt ccaacccagg cgatatcgtt
     2161 ctcgacccgt ttgccggtag ctttactacc ggtgccgtag ccatcgccag cggacgaaaa
     2221 ttcattggta ttgagatcaa cagcgagtac atcaaaatgg ggcttcgacg gctggatgtc
     2281 gcgtcgcatt actccgcgga agaactggcg aaagtgaaaa aaagaaagac cggcaacctg
     2341 tcaaaacgaa gccggttaag cgaagttgac cccgatctca ttgcaaagta aaggaagtgt
     2401 aagcctggtt ttcagattat tcatttcgtg tatatttcct gccagacttg gttaaacatg
     2461 cacaggctca ggtaatgatt cgcaagtatt ggtggctcgt cgttttcgct gtcttcgttt
     2521 ttctgtttga tactttactg atgcagtgga ttgaactgct gacaacagaa acagacaaat
     2581 gccgtaatat gaactcagtt tatccactaa aactggttaa ctgtgacgaa ctgaattttc
     2641 aggacagaat gtgaatttac atgacactta attcatttgt ttgatgaatt aatttcgtta
     2701 tgttttcatc tggcatgaac attcttaata cgttatcgac cagcgcgggt gcttgtttat
     2761 aaagatcata acccgccata ttcattaacc agttttgaac aattccgctg aaggcaccat
     2821 caataataat catcacaaca tctaaatcga ggttatttgc tacacaacct tgttgctgac
     2881 acgcctgcaa tacttcgcgg agagtctgcg gattaaagcc catcttttcg cgtatcactc
     2941 cctcggccag catcccatca ttaaattcac atttgtgata taagattttc agcaacgcct
     3001 gctggcgggg aattttggca atatattgca agccgacaat caatttttca cgcaattgtt
     3061 gaaacgggtc atgctctaat ccagccgtca agtgttcctg gattaactcc cgcaatgaag
     3121 gctgttgcaa ccacatctca ttaaacagtt gggtcttgtt ctcgaagtgc cagtagatag
     3181 cgccacgcgt aacgttagcg gcatcggcaa tgtcgttgaa cgtcgtcttg cttacgccat
     3241 gttgcgcaaa ctgggcgatg gcagtttcaa tcagttcttg ccgggtcttc agagcttcgg
     3301 ctttggttct ttttgccatg attaattatt caggaaataa atatattcga cacagggtga
     3361 gaaaatagcg aaggttaatc tatcatctaa tgtgtattta
//
LOCUS       DAZOYL010000341         3398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZOYL010000341 DAZOYL010000000
VERSION     DAZOYL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.342"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(252..1571)
                     /locus_tag="ACHUOY_003763"
     CDS             complement(252..1571)
                     /locus_tag="ACHUOY_003763"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR0684406.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1702..3237)
                     /gene="gadC"
                     /locus_tag="ACHUOY_003764"
     CDS             complement(1702..3237)
                     /gene="gadC"
                     /locus_tag="ACHUOY_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR0684407.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT          897 a          817 c          835 g          849 t
ORIGIN      
        1 ggacagtttc aatctacgct actgttcttc agaagagtat agcccatcgt aattattttt
       61 cggtgacagc gaatatcgta tggtttttca tattcataca tttttattag ggatttatag
      121 ctgtttaact aagtgtggtt aattgcgtgg ttaatttgac ttatgtaagc atgattacta
      181 gtgggatatt tcacaaggtg taacaagccg gtgggtaaag caccggcttg ttacaaagta
      241 agcatgggag tttaactgcc ccaacgactt tgcagatagc tgaccgcttg ttgagtctgc
      301 ggtttattca gatagtcctc acggaacaag atggtgccgc taatttctgg taccgcatcg
      361 ttaagatcga gctgcttttt cagttccggt acgccgccgt taatcatcca gtctggctct
      421 atctttgaag gttcacccac tttatagaag gcgataccga tatacaggcg ggtcctagtc
      481 ggtttaacga catccgccca ccattttgcc aacacgtcat aacgcgcggc actccgtgag
      541 aacggccagt aaatttgggg agcaatgtaa tccagcaatc cttgttccac ccatcgacgg
      601 gtgtcagcgt aggattcgtc ataggctgcc gcgcctcggg tatcggaacc gagcggatcg
      661 tgtgatcggt tacgccacac gcctgccggg ctaacgccaa attcgactcc cggcttaatg
      721 cttttaatgg tgtgcgatac ttttgcaatt aactgctgag tattgttgcg ccgccagtct
      781 gcttttgacg caaatgcgcc tccgtatttg cggtacgttt cgttatcatt tagccgtgaa
      841 cccggtgact cagtatagaa atagtcgtca aactgcacgc catctaccgg atagcgggaa
      901 accacttctg cgactattga tgtgatccag tcctgaacct cagggatgcc cgggtcgagg
      961 acaaagcgat cgccagacgt tctgatccag tcacggtgtt gcacatagac gctcgccggt
     1021 tgttgagaca gagtgctatt cagttccctg atagtaccgg gcttcgtatt aaccgatacg
     1081 cgataggggt taaaccaggc gtgtactttc atcccacgct tgtgggcttc gtcgagcata
     1141 aattgcagcg gatcgtaacc cggattttca ccaatcttac cggtcataag atcggaccat
     1201 ggcaaaattt tcgatggcca cagggcggta ccgtccggct taacctggaa aaagaccgtg
     1261 tttataccga gacgttgcag atgatccagt ttgtcgatca tcgcctgttg ttgtacacgg
     1321 gcccggctgg tggggttact aatgttaacc gaggaaaccg gtggccagtc gaggcgagac
     1381 accgtggcca gccagatgcc acgcatcggt tgtgacgatt gttgcgtcgt ggctggtggc
     1441 tttgaacctg ctggtggtgt caccatggac tctggaggcg tgcttttaca actacacagt
     1501 aaaagtgcca gtgcaaccag tatcgctggt cttctaatcg ctaatttctc gtttcgggag
     1561 cagatatcca tatatgctgg tttccggtaa acagatgtgc gctcattctc gtacttatcc
     1621 cggttaagtc aatacgacag cgagcacgaa aaagggagcg atgaattatc gctcccttgt
     1681 cttataacca ttcagacatg gttagtgttt cttgtcattc atcacaatat agtgtggtga
     1741 acgtgcacgc gggtgcagga agaagtgacc ttttggtgcg ttctgactgt tgattggctc
     1801 cagagtgacg ccggtattcg ctttgccttt acgatcatga acagcataga gaataaaggg
     1861 cagggcaagt accaccagga aactaacgac cagtaattca acatacatat cggtagagtc
     1921 accctggatg ttatccggcg gcaggaagga aacaataaac gccataattg aagtcagcag
     1981 accgacaata gccacgacca gtttcacccc tttaccacca gggatattaa atgtgcgttt
     2041 taagtcagga tgtttaagaa ccaacacaat gtagccaata aacagcatga aataagcaca
     2101 cagataaatc accaccgtca gcgccagtgc gatcaggaag gacatgttgt taccgccacc
     2161 ggtattggtg aggatgatca acgcgataga agtaatcacc agctgtgaaa tgaccagcgt
     2221 taccggtacg ccatttttgt tcattttagc gaatgccgct ggcagcaggt ttttctgcgc
     2281 tgtgacatac atcccgcgag aaggaccaac aatccaggag gcgatttccg ccagaacacc
     2341 cagcaacagc agtgcggaga tcacgcgaac cgtccactca atttccggtg ccacatggga
     2401 catcagaacg gtaaaggttt gcattacccc tgcggagagg ttgatttcat tacccggaat
     2461 gaccatcgca atagacaaac caccaacaga gcttaagcag attgccgcta ccatcagcag
     2521 taacatagcc aacggatagt cgcgccctgg gttgctcatt tcattgacgt gggttgcgga
     2581 tgcttctacg cccatataac tcaaaatgaa ggcaacaaat acaaccaggg tgcccacttt
     2641 agagaagtca gggaagaagg tctttgaatc catttcgata gcaacggggg caccggagtg
     2701 cagataaata gccgctaatg cgatcaaaat aaatgcaggt aacaggatac cggcgaagaa
     2761 gccaacttta gcaattcgtg ccgtgtattt cgtgccacca aactgcgtta atgccagcgc
     2821 ccaaagaatg atgagtgctg caatagtttt ggtaatgggg tcttcattca gcgctggcca
     2881 tttcaggatg taggagagtg cccctaacac gaaatagagc atcggaataa aaccaatggc
     2941 gatttgcaga tagccaaatg agatcgctgc aaatccccat ctcggcccca gagtatttga
     3001 tacccaggcg aagacaccac cttcttccca gccgtcgacg gtggccattt ccgcagcaca
     3061 aagtcccacg ggaataaacc ataaaatccc gcctaatagc aggaagaaga ctaatgaaaa
     3121 gcccgatgtt gcgaaggtag ggtattcata aacagccatt accatcgatg ccgttatggc
     3181 aaaaaagccg agtaatgtga gctgcttagc tttacctgtc tgtactgatg tagccatatt
     3241 atccccctaa aacggtattc ctgtcggaac cgcacctgtt ttgttctgaa gcgtattcag
     3301 aacaatattt tccgttgcta atgccagtga acagactttg gaaattatcc cgaaacgggt
     3361 tcgtttcggg acaccgttac cgttaaacgt tatcaggt
//
LOCUS       DAZOYL010000342         3347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZOYL010000342 DAZOYL010000000
VERSION     DAZOYL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.343"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHUOY_003765"
     CDS             complement(<1..312)
                     /locus_tag="ACHUOY_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR0684408.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUOY_003766"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUOY_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR0684409.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUOY_003767"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUOY_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR0684410.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT          908 a          872 c          728 g          839 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgc
//
LOCUS       DAZOYL010000343         3305 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZOYL010000343 DAZOYL010000000
VERSION     DAZOYL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.344"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="ACHUOY_003768"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="ACHUOY_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="ACHUOY_003769"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="ACHUOY_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR0684411.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..>3305
                     /gene="sfmF"
                     /locus_tag="ACHUOY_003770"
     CDS             2834..>3305
                     /gene="sfmF"
                     /locus_tag="ACHUOY_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR0684412.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQP"
BASE COUNT          813 a          750 c          853 g          889 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc ttgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccag
//
LOCUS       DAZOYL010000344         3289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZOYL010000344 DAZOYL010000000
VERSION     DAZOYL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.345"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..348
                     /locus_tag="ACHUOY_003771"
     CDS             <1..348
                     /locus_tag="ACHUOY_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0684413.1"
                     /translation="NVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTS
                     QRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQSGRP
                     LKQEPTEMIQATA"
     gene            complement(388..1059)
                     /locus_tag="ACHUOY_003772"
     CDS             complement(388..1059)
                     /locus_tag="ACHUOY_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR0684414.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1166..1399)
                     /gene="yedF"
                     /locus_tag="ACHUOY_003773"
     CDS             complement(1166..1399)
                     /gene="yedF"
                     /locus_tag="ACHUOY_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR0684415.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1396..2601)
                     /gene="yedE"
                     /locus_tag="ACHUOY_003774"
     CDS             complement(1396..2601)
                     /gene="yedE"
                     /locus_tag="ACHUOY_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR0684416.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2788..3201
                     /gene="yedD"
                     /locus_tag="ACHUOY_003775"
     CDS             2788..3201
                     /gene="yedD"
                     /locus_tag="ACHUOY_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR0684417.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT          845 a          872 c          873 g          699 t
ORIGIN      
        1 aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg gtatgaaatg
       61 cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc tgttccgcca
      121 gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa ccgcctgtca
      181 caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt aaatggcgct
      241 cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt gcagtcaggc
      301 cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac gtagcaggga
      361 tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact ccgcgccctg
      421 gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt ttgctggcac
      481 acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc gcatccattc
      541 ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta ccagaccctg
      601 gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct caaatggtgt
      661 gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa aaaaaggctg
      721 ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga ttgcccgacc
      781 atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc gggcgttaat
      841 cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat ccagatgaag
      901 gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt tgtatctgcc
      961 aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa gcgcaaggta
     1021 atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct gagatcatac
     1081 tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca atgttcccgc
     1141 tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg gtcggcccgt
     1201 cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga atattattga
     1261 tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac tgcggcatcg
     1321 cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca aggcggtaat
     1381 caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac ggcggaagaa
     1441 gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat atgtcaccag
     1501 caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt cggtggccag
     1561 cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca cattgcccag
     1621 accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc cggtttcgca
     1681 gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg cgtttggtcc
     1741 cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc cgatggcact
     1801 caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga tccacatatc
     1861 gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa agccgacgcc
     1921 aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg cccagcccag
     1981 catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat ccggtttttg
     2041 cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga agatgggcag
     2101 cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa agaaccaggc
     2161 atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc ccattgccag
     2221 acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc ggatacggct
     2281 gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc agccgccaaa
     2341 catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca gatggataat
     2401 tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct gaccgcccca
     2461 acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat agtaagtgga
     2521 gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca accaggcgtg
     2581 tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg aaacaagaat
     2641 cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa ataatagtta
     2701 gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa tgccacaatc
     2761 ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg tgcgctcatg
     2821 ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac ccccgcgccg
     2881 gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt cagtccggaa
     2941 gagataggca gcctgattgt cactaaagag ggcgataccc tggattgccg tcagtggcag
     3001 cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac taacgttacc
     3061 gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta cgatggtatg
     3121 acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga taaagcgccg
     3181 ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat gaatttaaat
     3241 cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaag
//
LOCUS       DAZOYL010000345         3287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZOYL010000345 DAZOYL010000000
VERSION     DAZOYL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.346"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            90..518
                     /gene="uspC"
                     /locus_tag="ACHUOY_003776"
     CDS             90..518
                     /gene="uspC"
                     /locus_tag="ACHUOY_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR0684418.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(525..1949)
                     /gene="otsA"
                     /locus_tag="ACHUOY_003777"
     CDS             complement(525..1949)
                     /gene="otsA"
                     /locus_tag="ACHUOY_003777"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR0684419.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1924..2724)
                     /gene="otsB"
                     /locus_tag="ACHUOY_003778"
     CDS             complement(1924..2724)
                     /gene="otsB"
                     /locus_tag="ACHUOY_003778"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR0684420.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2891..>3287)
                     /gene="araH"
                     /locus_tag="ACHUOY_003779"
     CDS             complement(2891..>3287)
                     /gene="araH"
                     /locus_tag="ACHUOY_003779"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0684421.1"
                     /translation="RNTLAIGGNEEAARLAGVPVVRTKIIIFVLSGLVSAIAGIILAS
                     RMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVENAMNLLNIS
                     PFAQYVVRGLILLAAVIFDRYKQKAKRIV"
BASE COUNT          826 a          829 c          782 g          850 t
ORIGIN      
        1 acctttttga ccgagttggg attgcgtcgt ttctccatta ggagtaaagc tttaatgtca
       61 cctgaagttc acagaataag gaacaggtta tgagctatag caatattctt gtcgctgttg
      121 cggttacacc ggaaagtcag caactgctgg caaaagcggt atctatcgcc aggccagtaa
      181 agggacacat cagtttaatt actctcgctt ccgacccgga aatgtacaat caattagccg
      241 cgccgatgct tgaagattta cgcagcgtga tgcaagaaga aacgcaaagc tttcttgata
      301 agttcattca ggatgcgggg tatcccgttg acaagacgtt tattgcctac ggtgagttaa
      361 gcgaacatat tctggaagta tgtcacaaac atcatttcga tttggtgatt tgcggtaatc
      421 acaatcacag tttcttttcg cgagcatcct gctcggcgaa aagagtgatt gcctcaagtg
      481 aggtcgatgt gctgttagtt ccacttacgg gagattaacc gctcctacgc aagctttgga
      541 aaggtagcaa ctttatcgcg ctgctggctt tccgcgcttc gcggaactat ctgctttagg
      601 tcgctaatga agcactcctg ccagtggtta atatcgtttt tcacgataac gtccagcatt
      661 tctgcatgac gggaaatacg ttccgccagc gacatagtca atgcacgatc cagcgcagct
      721 gcaacttcgt cacgatcgta ggggttaaca attaacgccg acgttaactc gtttgccgct
      781 cccgcaaatt gcgaaagaac aagaacgccc gggttggctg ggtcctgagc agcaacatac
      841 tcttttgcta ccaggttcat cccgtcacgc agtggcgtca ctaagcccac gtcagagtag
      901 cggaatattt tcatcagtaa tttacggtca aaatgctgat tcaaataata aagcggcgtc
      961 cagcctaatt gcccgtattt accattaatt cgtccagctt cattttcgag ctgatgacga
     1021 atatcctgat aggcttgcac atcaccacgc gacgttggtg caatctgggt ataacgaatt
     1081 ttaccatgat gctgcggata tttttccagc aacgcttcat aggcgagaaa acgctctggc
     1141 aaacctttgg aataatccag ccgttcgaca gaaaagatat tttgtacgtt tttcagttcc
     1201 gctttaagtt gcgccagttt tggcggcagt ggcccggcag cctgtttggc tatttctttc
     1261 ggttcaatgc cgatcgggta gacttctgtt cgaaatgctt tgccccaggc tgtatggctt
     1321 tttgcgctac gtgtcgtgac gcgggtcagg ttagaaagac aatccaggaa cgccagacga
     1381 tcgttttctg tctggaaacc cagcaaatca taatcacaaa gctgttcaag caaggtgtca
     1441 tatgtcggca gcgcgttgaa gatttccggt gtcgggaaag gaatatgcag aaagaaacca
     1501 atgcgattat tcactccccg tttgcgtaat tcatgcgcaa atggcaacag gtgataatcg
     1561 tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc cagcaacgca
     1621 tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcaccagatc gagccgataa
     1681 tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc aaggtcctgt
     1741 tcgctgaggt taaaagaggc ccacgtaatg ttaccttttt tcaccttttt tagcggctga
     1801 tcctcattcc ctgtttcacc actccagcca aaccacagtc cgcctgcggc tttcagtgcc
     1861 cccagtatgc caacggcaag gccaccggca ctggcggcgt gctcgtctgg tggtgcaatc
     1921 cggttagata ctacgactaa acgactcata gtcatcactc ctgttatttt ctcttttttg
     1981 ttgtaatgcg gtggttatca tttcaagcca gctccagaca tccggcacac ccgccagtcg
     2041 ccatgatgcc tgagttgcac ctgtgccaat ttttactgac attccgccca gtcggttaac
     2101 gactgcgaag ccagattcat cggttaaatc atcgcccaga aatacgggcg ttcgcccgat
     2161 aaagggagct tcctgcataa aagctgcaat tgcctcacct ttactggtac ctctcggttt
     2221 gatctcgaca acacactttc cctgctgtaa cgccatttgt ggccagatct gagtaatacg
     2281 ttgcgctaat gtcattaatg cgtcttcatg ctgcggagcc tgacgataat gcagcgcaaa
     2341 agccatccct ttcgcctcca gctccgcgcc gggatactga gcgatgactg tatgcagttg
     2401 cacgctaata tcacgcgcaa tcgcatccgg cagatgaacg atatgtgttt taccattgat
     2461 gtcacggcgc tccgccccat gcacgcccgc taacgggaag cgataaggtt ttgccagtgc
     2521 gtcaagctcc accattgagc gccctgatat caatgccaat gcaccatcac ttgcggttgc
     2581 cagtagctgt agtccttgca gaatattgtc aggcacgacg acctgatcgg gatgcggttt
     2641 gatttccgcc agcgttccat caagatcaaa aaaccaggca tatttcgcgg atagttcagg
     2701 ggtttcggtt aacggttctg tcacccggct ctcctccttc tcttttctta ttgttttgcg
     2761 cagaacgcgc tttacagaca tgtaagtata gacagtgtga cgggggtcgc catttgcaat
     2821 gaagaacagc caaccgttgt ggcggctggc tgtggtggga aaaaacgtta aattgttgtg
     2881 gaaaaaagca tcagacaatg cgtttcgctt tttgcttgta acggtcgaag atcaccgctg
     2941 ccagcaggat taagccgcga accacgtact gcgcgaaagg agaaatatta agcaggttca
     3001 tggcgttttc cacggtgcct aaaattaaga tacccgccac cacatatgag atttttccga
     3061 tgccaccttt cagagaaacg ccacctaaaa cgcaggcgga gataacaata agctcataac
     3121 caatcgacgt cattggctgc ccactagtca tacgtgaagc cagaataatt ccggctatcg
     3181 cagataccag gccagagaga acaaagataa taattttggt gcgaacaacc ggtacacccg
     3241 ccagacgcgc agcctcttcg ttcccgccaa tcgccagggt gttacga
//
LOCUS       DAZOYL010000346         3285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZOYL010000346 DAZOYL010000000
VERSION     DAZOYL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.347"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..229)
                     /locus_tag="ACHUOY_003780"
     CDS             complement(<1..229)
                     /locus_tag="ACHUOY_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684422.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            403..1116
                     /locus_tag="ACHUOY_003781"
     CDS             403..1116
                     /locus_tag="ACHUOY_003781"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0684423.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1148..1906
                     /locus_tag="ACHUOY_003782"
     CDS             1148..1906
                     /locus_tag="ACHUOY_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684424.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1952..>3285
                     /locus_tag="ACHUOY_003783"
     CDS             1952..>3285
                     /locus_tag="ACHUOY_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR0684425.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          750 a          790 c          854 g          891 t
ORIGIN      
        1 atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
       61 acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt
      121 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
      181 aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct
      241 ttaatttaaa catccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat
      301 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
      361 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      421 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      481 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      541 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      601 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      661 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      721 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      781 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      841 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      901 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      961 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
     1021 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
     1081 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
     1141 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
     1201 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
     1261 caccgatctg gtgaaatcat tgcaacagca ctggcaaacc gagcggcgct atctggggct
     1321 gctctcgggc gaagacggcg acaaaatcaa acgattaccg ttggttggcg tctggctgga
     1381 acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
     1441 atgggcggcg gtgattatgt actgcttatc gctgcgaaga atggcgaatc tacgcaaggt
     1501 tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
     1561 ttcgggcttt tattacgacg gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag
     1621 catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat atcgcccgga
     1681 attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc ctgaccatga
     1741 ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata acggcacaaa
     1801 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc
     1861 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac
     1921 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
     1981 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
     2041 cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     2101 catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     2161 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     2221 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     2281 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     2341 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     2401 gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac
     2461 gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc
     2521 gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt
     2581 tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga
     2641 gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     2701 catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc
     2761 tgccgccggg ttatttaaaa tcgccatgac attctttgac gccgccggaa cgccagcggg
     2821 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     2881 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     2941 cgcggtgttg atcgccggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     3001 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc
     3061 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     3121 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     3181 agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac
     3241 tttaaaggca ttttttcagc gtcattcctt gctttataac gcagc
//
LOCUS       DAZOYL010000347         3279 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZOYL010000347 DAZOYL010000000
VERSION     DAZOYL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.348"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            165..881
                     /gene="nanC"
                     /locus_tag="ACHUOY_003784"
     CDS             165..881
                     /gene="nanC"
                     /locus_tag="ACHUOY_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR0684426.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            901..2007
                     /gene="nanM"
                     /locus_tag="ACHUOY_003785"
     CDS             901..2007
                     /gene="nanM"
                     /locus_tag="ACHUOY_003785"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR0684427.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2072..3052
                     /locus_tag="ACHUOY_003786"
     CDS             2072..3052
                     /locus_tag="ACHUOY_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR0684428.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3162..3279
                     /locus_tag="ACHUOY_003787"
                     /pseudo
     CDS             3162..3279
                     /locus_tag="ACHUOY_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          975 a          693 c          768 g          843 t
ORIGIN      
        1 atgtggaaag agaattttca caggagacct tatacaaaaa tataaaataa gctacccgtt
       61 gccaaagaca ctataagcct ggcaaaaaat attacacaac ataaatgcta attgtttatg
      121 cgggctttgt attgctttct gtatcctaca aatgagtgaa atttatgaaa aaggctaaaa
      181 tactttctgg cgtattatta ctgtgctttt cgtcaccatt aatttctcag gctgcgacac
      241 tggacgtacg tggtggatat cgtagcggaa gccatgccta tgagactcga ctcaaagtca
      301 gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag taatacctgg aataccattc
      361 atgataataa aaaggaaaat gccgcactca atgatgttca ggttgaagtt aattacgcga
      421 ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt aacgcatttt agcagcaacg
      481 gcacacgcta cggaccctac gtaaaactgt cctgggacgc gacaaaagat cttaattttg
      541 gcattcgcta tcgttacgac tggaaagctt accgacaaca agacttatcc ggtgatatgt
      601 ctcgtgataa cgttcatcgt tgggatggat atgtcactta ccatattaat agtgatttca
      661 ccttcgcatg gcaaacgacg ctatacagca aacagaatga ttatcgctat gcaaaccaca
      721 agaaatgggc gacggaaaat gcctttgttc tacaatacca tatgacgccc gatattacgc
      781 catacataga atatgactac cttgaccgcc agggtgttta caacggcaga gataatttat
      841 cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta gtagacagga gacagtcaca
      901 atgaataaaa ctataatggc gcttgctatc atgatggctt catttgccgc aaacgcgtct
      961 gtattaccgg aaactcctgt gccatttaaa agtggtaccg gagcaattga taacgacact
     1021 gtctacattg gtttaggtag cgcaggtaca gcatggtaca agctagatac acaggccaaa
     1081 gataaaaaat ggactgcgtt agctgcattc cctggtggac cgagagatca agcaacctct
     1141 gcatttattg atggcaatct atatgtgttt ggcggcattg gcaaaaacag cgagggattg
     1201 actcaggtat ttaatgacgt acacaaatac aaccccaaaa ccaatagctg ggttaaattg
     1261 atgtcgctcg cgccgatggg catggcgggt catgtgactt ttgtacacaa cggcaaggct
     1321 tatgttactg gcggtgttaa ccagaatatc ttcaatggct attttgaaga tctcaacgag
     1381 gctggaaaag attcaaccgc tatagataaa atcaacgccc actattttga caaaaaagca
     1441 gaagattatt tcttcaataa gtttctgttg tcttttgatc cctcaacaca gcaatggagt
     1501 tacgctggcg aatcgccctg gtacggaacg gctggtgcgg cggttgtgaa taaaggtgat
     1561 aaaacctggc ttattaatgg cgaagccaaa ccaggattgc gaacggatgc cgtatttgaa
     1621 cttgatttca ccggtaataa tttaaaatgg aataagcttg atcccgtctc atcaccagat
     1681 ggcgtcgctg gcggttttgc ggggataagc aatgattctc ttatatttgc cggaggggcc
     1741 ggattcaaag gttcacgaga aaattaccag aacggtaaga actatgcgca tgaaggcctg
     1801 aaaaaatcat atagcactga tattcatctt tggcataacg ggaaatggga taaatcgggt
     1861 gaattatcgc aaggtcgggc ctacggagta tcattgccct ggaataatag tttattaatt
     1921 attggcggtg aaactgcagg cggcaaagcg gtgacggatt cagttttaat ttctgtgaag
     1981 gataataaag tcacagtaca aaactaacgc ttcagggccc cggtaaaggg gccctgctat
     2041 caatttgtca tttgtattac gcgaaaatta tatgaacgca ataatatcgc ccgattatta
     2101 ctatgttctt accgttgctg gtcagtctaa tgccatggcg tatggcgaag gactgccatt
     2161 accggacagg gaagatgcgc ctcattccag aattaaacaa ttagcgagat ttgcgcatac
     2221 gcatcccgga ggcccgccat gtcactttaa cgacattatt ccactgactc actgcccaca
     2281 cgatgttcag gatatgcagg gttatcacca tcctctggca acgaatcatc aaacacagta
     2341 cggcaccgtt ggccaggcac tgcatattgc acggaaatta ctgcccttca ttcctaataa
     2401 tgcagggatt cttatcgttc cgtgttgccg tggcggatcg gcttttaccg cgggcagcga
     2461 agggacatat tcagaacggc acggagccag ccatgatgct tgtcgttggg gaacggatac
     2521 tccgctgtac caggatttag tcagcagaac gcgagccgca ctggcaaaaa atccgcagaa
     2581 caaattcctc ggcgtatgct ggatgcaagg cgaatttgac ttaatgacca gtgactacgc
     2641 gtcacaccct caacacttta atcatatggt tgaagccttt cgtagggatc taaaacaata
     2701 ccattctcag cttaataata ttactgacgc gccatggttt tgcggcgata ccacctggta
     2761 ctggaaagaa aatttcccgc attcgtatga agctatttat ggcaattatc aaaataatgt
     2821 tttagccaat attattttcg tcgacttcca gcaacaaggt gaaagaggac tgacgaacgc
     2881 gcctgatgaa gacccggacg atttaagcac gggatattac ggttcagcgt accggtcacc
     2941 ggagaactgg acgacggcac tgcgaagcag tcatttcagc acggcagccc gtcgggggat
     3001 tatttctgac agatttgtag aagcaatttt gcagttttgg cgcgaaaggt gagcggggag
     3061 tttattcatg ggggatgtcc ttgttcgttc tcagagtaga caacaaaaaa caaataagat
     3121 caaggtaata tctcttcact tgcgggctcg aacagcaggc aattgccaat ataataatct
     3181 ggcacaaggg tgaaaaacgc gcaccgcata aaccgttgtt attgcttaca gattagccgg
     3241 atgccttaat agacatccgc gccttttcga ttatggctc
//
LOCUS       DAZOYL010000348         3275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZOYL010000348 DAZOYL010000000
VERSION     DAZOYL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.349"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..2940
                     /gene="sigA"
                     /locus_tag="ACHUOY_003788"
     CDS             <1..2940
                     /gene="sigA"
                     /locus_tag="ACHUOY_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR0684429.1"
                     /translation="KNFTNTMQLDGKKQELSSIIKDKDLSVSGGGELTLKQDTDLGIG
                     GLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGDNLHKIGSGTLDVKIAQ
                     GNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSESGNGEIYFTRNGGTLDL
                     NGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGNVSGNISINHIINTTQQ
                     HNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGNNNCPIPFLCQKDYSAA
                     IKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNLENATLSIGRDANVKGH
                     IEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSAGESSFNGSLSAQNSKI
                     TVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIELAGTLTLTGTPTENNK
                     YSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILFGVDQYKEDNLDKTTHT
                     PLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQSLKTRDSLLLFSNIENS
                     GFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGSNNSLSVDIQKVGNNNS
                     GLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEHTTWTLTGYKVAENTAS
                     SGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARIMSGAGSASSGYSDNYT
                     HVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSVGAGLYASAIFDSGAYI
                     DLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHVTEDSWIEPQAELVYGA
                     VSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLFA
                     NGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLEFEKSAFGKYNVDNAIN
                     ANFRYSF"
     gene            2999..>3275
                     /locus_tag="ACHUOY_003789"
     CDS             2999..>3275
                     /locus_tag="ACHUOY_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684430.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVS"
BASE COUNT         1057 a          667 c          732 g          819 t
ORIGIN      
        1 aagaatttca caaacactat gcagctggat ggtaaaaaac aagagttatc atcgattata
       61 aaagataagg acctgtctgt ctcaggagga ggggaattaa cgctcaagca ggataccgat
      121 cttggcattg gcgggcttat attcgataag aaccagacat ataaagtgta cggaaaagat
      181 aagtcttata aaggtgccgg gatagatatt gataataata ccaccgttga atggaatgtt
      241 aagggcgttg ccggagataa tctgcataaa ataggtagtg gtactctgga tgtaaaaata
      301 gcacagggaa ataaccttaa aataggtaat gggactgtca tccttagtgc tgaaaaagcc
      361 ttcaataaaa tttacatggc cggaggtaaa ggtacggtaa aaataaatgc caaagacgct
      421 ttaagcgaaa gcggtaatgg cgaaatctat tttaccagaa atggcggaac actggatcta
      481 aacggctatg accagtcatt tcagaaaatc gcagcaacag atgcgggaac aaccgtaacg
      541 aactcaaacg tgaagcaatc aacattatca cttactaata ctgatgcata tatgtaccat
      601 gggaatgtat caggtaatat aagcataaat catattatca atactaccca gcaacataac
      661 aataatgcca atctgatctt tgatggctca gtcgatatca aaaacgatat ctctgtccgg
      721 aatgcacagt taacattaca aggacatgcg acagaacatg ccatatttaa agaaggcaat
      781 aacaactgtc caattccttt tttatgtcaa aaagactatt ctgctgccat aaaggaccag
      841 gaaagcactg taaataaacg ttacaatacg gaatataagt ccaacaatca gatagcctct
      901 ttttcccagc ccgactggga aagtcgtaaa tttaatttcc ggaaattaaa tttagaaaac
      961 gcaaccctga gtataggccg ggatgctaat gtaaaaggac acatagaggc taaaaactct
     1021 caaattgttc tgggaaataa aactgcatac attgacatgt tctcaggaag aaacattact
     1081 ggcgaaggtt ttggattcag acaacagctt cgctccgggg attcagcagg cgaaagtagt
     1141 ttcaacggca gtctgagtgc tcaaaacagc aaaataactg ttggtgataa atcaactgtt
     1201 actatgactg gtgcattatc cttaattaat acagacctga ttatcaacaa aggagctact
     1261 gttaccgccc agggaaaaat gtatgtagat aaagctattg aactggccgg aaccctgaca
     1321 ttaacaggca cccctacaga aaataataaa tacagcccgg caatctatat gtcagatgga
     1381 tataatatga cagaagatgg tgccacgtta aaggctcaaa attatgcctg ggtcaatggt
     1441 aatataaaat cagacaaaaa agcatctatt ctgtttggtg ttgaccagta taaagaagat
     1501 aacctggaca aaaccacaca cacaccgctg gctacaggtt tgctgggtgg ctttgatact
     1561 tcttataccg gaggtattga tgctcctgct gcctcagcca gcatgtataa caccttatgg
     1621 agagtaaacg gacagtcagc cctgcaatca ttaaaaaccc gcgacagtct tttgttgttt
     1681 agtaacatag agaattcggg tttccatact gtgacagtaa acacactgga tgccactaat
     1741 actgctgtga ttatgcgggc tgatctgagc cagtctgtaa atcaatcgga taaactcatt
     1801 gttaaaaatc agttaaccgg aagcaataac agtctgtcgg tcgatataca gaaagtggga
     1861 aataataact caggattaaa cgttgacctg ataacagccc caaaaggaag caataaagag
     1921 atatttaaag ccagtactca ggccataggt ttcagcaaca tatctcctgt gatcagcacg
     1981 aaagaggatc aggaacatac cacgtggacc ctgaccggat ataaggtggc tgaaaataca
     2041 gcatcttccg gtgcagcaaa atcgtatatg tccggtaatt acaaagcctt cctgacagaa
     2101 gtcaacaacc tgaataaacg aatgggggat ctgcgtgaca ccaatggcga ggccggtgca
     2161 tgggcccgca tcatgagcgg agcaggttca gcttctagtg gatacagtga caactacacc
     2221 catgtgcaga ttggtgtgga taaaaaacat gagctggatg gacttgacct tttcactggt
     2281 ctgactatga cgtataccga cagtcatgcc agcagtaatg cattcagtgg caagacgaag
     2341 tccgtcgggg caggtctgta tgcttccgct atatttgact ctggtgccta tatcgacctg
     2401 attagtaagt atgttcacca tgataatgag tactcggcga cctttgctgg actcggaaca
     2461 aaagactaca gttctcattc cttgtatgtg ggtgctgaag caggctaccg ctatcatgta
     2521 acagaagact cctggattga gccgcaggca gaactggttt atggggccgt atcaggtaaa
     2581 cggttcgact ggcaggatcg cggaatgagc gtgaccatga aggataagga ctttaatccg
     2641 ctgattgggc gtaccggtgt tgatgtgggt aaatccttct ccggtaagga ctggaaagtc
     2701 acagcccgcg ccggccttgg ctaccagttt gacctgtttg ccaacggtga aaccgtactg
     2761 cgtgatgcgt ccggtgagaa acgtatcaaa ggtgaaaaag acggtcgtat tctcatgaat
     2821 gttggtctca acgccgaaat tcgcgataat cttcgcttcg gtcttgagtt tgagaaatcg
     2881 gcatttggta aatacaacgt ggataacgcg atcaacgcca acttccgtta ctctttctga
     2941 taacagcccg ggccgcgttt gcggcccttc ttctaccgta gagaatatgt attaccctgt
     3001 gacagactat atcgctcttg ctctcattat tagctttctt tttctgacat tatttatctg
     3061 cctgttatgc cttaaacatg agcgaataaa aaaagaaact atcaggcaaa aaaatgcaca
     3121 tattctggag catggctgga atgcaactga gttctcatgg ttccgatacg gacagtataa
     3181 cgaaacgggt atttacatct cgatcgagaa gaccattatc attacaatca ccgtttctgg
     3241 tgagtgcttt aaaaaagaat acagcattgt ctccc
//
LOCUS       DAZOYL010000349         3273 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZOYL010000349 DAZOYL010000000
VERSION     DAZOYL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.350"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHUOY_003790"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHUOY_003790"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR0684431.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHUOY_003791"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHUOY_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR0684432.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHUOY_003792"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHUOY_003792"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR0684433.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHUOY_003793"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHUOY_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR0684434.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          771 a          868 c          779 g          855 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     3181 gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat
     3241 gacaaaggaa atactttgcg caaatatttt ttg
//
LOCUS       DAZOYL010000350         3229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZOYL010000350 DAZOYL010000000
VERSION     DAZOYL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.351"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            284..1003
                     /locus_tag="ACHUOY_003794"
     CDS             284..1003
                     /locus_tag="ACHUOY_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR0684435.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            1014..2441
                     /locus_tag="ACHUOY_003795"
     CDS             1014..2441
                     /locus_tag="ACHUOY_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR0684436.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2434..3129
                     /locus_tag="ACHUOY_003796"
     CDS             2434..3129
                     /locus_tag="ACHUOY_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR0684437.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
BASE COUNT          846 a          677 c          917 g          789 t
ORIGIN      
        1 gaataagtag ctctgaatag gtataaggga tgtagccttt ttaaatggat ttcttatgcc
       61 aaaaatgaac gtcgatttcc ccataaaatg tgagcgatgc cgaaagaaat aaaattagtt
      121 attgcatttg aagtttggat gaaagattat catttgtcac acaaatgagg ggtggtatgt
      181 tgctagccaa ttaaaaaaga acgccatatt tattgatgat tgatcccccg gaaggagggg
      241 aaccatcggg cataaatggg catgaagtaa tggagtatta gttgtgaatg tcaatttctt
      301 tgtcacctgt attggtgacg ccctgaaatc aagaatggca cgagactccg tgctgctact
      361 ggaaaaactc ggctgtcgcg taaatttccc ggagaaacag ggatgctgcg gtcagcctgc
      421 gatcaatagc ggttatatca aagaagcgat tccagggatg aaaaatctga tcgccgcact
      481 ggaggataac gacgatccca ttatttcacc ggctggctct tgcacctatg ccgtaaaaag
      541 ttacccgacg tatctggcgg atgaacctga atgggcatca cgtgccgaaa aggttgccgc
      601 gcgtatgcag gatctcacct cttttattgt taataaatta ggggtagtcg atgtaggtgc
      661 cagtttgcaa gggagagctg tgtatcaccc atcttgtagc ctggcccgta agctgggagt
      721 gaaggacgag ccacttacgc tgctgaaaaa tgtgcgtgga ctggagctgt tgacctttgc
      781 tgaacaggat acctgctgcg gatttggcgg cacgttctcg gtcaaaatgg ccgaaatatc
      841 cggcgagatg gtgaaagaaa aggttgtgca cctgatggaa gtccgccctg agtatttaat
      901 tggtgctgac gtgagttgcc tgctgaacat cagtgggcga ttacaacggg aagggcagaa
      961 agtcaaagtg atgcatattg ctgaagtgtt gatgagccgc tgaggatata aagatgtcga
     1021 tcaaaaccag taatacagat tttaagacac gcatccgtca gcaaattgaa gatccgatca
     1081 tgcgcaaagc ggtggcaaac gcgcagcagc gtattggggc aaatcggcaa aaaatggtcg
     1141 atgaattggg gcactgggag gagtggcgcg atcgggccgc ccagatacgt gatcatgttc
     1201 tgagtaatct cgacgcttat ctgtaccagc tctcagaaaa agtgacgcaa aacggcggtc
     1261 acgtctggtt tgcaaaaacc aaagaagacg ctacccgcta cattttacag gttgcccaac
     1321 gcaaaaatgc ccggaaggtg gtgaaatcta aatcgatggt gaccgaagag attggtgtca
     1381 atcatgtgtt gcaggatgct ggcattcagg tgattgaaac cgatctgggt gaatatattc
     1441 tccagctgga tcaagatccg ccatctcatg ttgtggtccc ggcaattcat aaagatcgcc
     1501 atcagatccg tcgagtgcta cacgaacgtc tgggctatga ggggccggaa acgcctgaag
     1561 cgatgacctt attcatccgg caaaaaatcc gcgaagattt cctcagtgct gaaataggta
     1621 ttaccggctg taatttcgcg gtggcagaga caggttcggt atgcctggtg accaatgaag
     1681 gtaatgcgcg aatgtgtacc acgctgccta aaacgcatat tgctgtgatg ggaatggagc
     1741 gtattgcccc cacgtttgcc gaggtagatg tattgatcac catgctggcg cgcagtgccg
     1801 ttggtgcacg tttgacggga tacaacacct ggctgacagg accgcgcgaa gcggggcacg
     1861 ttgatggtcc tgaagagttt catctggtta ttgtcgataa cgggcgttct gaggtgctgg
     1921 cctctgaatt tcgggatgtg ctgcgctgta ttcgctgcgg ggcttgtatg aatacttgtc
     1981 cggcatatcg ccatattggc ggtcatggat atggctctat ttatccaggg ccaattggtg
     2041 cggtgatttc tccgctactt ggcggctata aagattttaa agatttaccc tacgcctgct
     2101 ctttatgcac tgcttgtgac agcgtgtgtc cggtgcgtat tccgctgtca aaactgattt
     2161 tgcgtcatcg tcgggtgatg gctgaaaaag ggatcaccgc aaaagcagag caacgggcga
     2221 taaaaatgtt cgcttatgcc aatagtcatc caggattgtg gaaagtcggg atgatggccg
     2281 gcgctcatgc ggcaagctgg tttatcaatg gcggcaaaac accactcaaa tttggcgcga
     2341 ttagcgactg gatggaagca cgcgatcttc ctgaagctga cggagagagt ttccgtagtt
     2401 ggtttaagaa acatcaggcg caggagaaaa agaatggata atcggagcga atttttgaat
     2461 aacgttgctc aggcactggg tcgcccgctg cgacttgaac cgcaagcaga agatgcgccg
     2521 cttaacaact atgctaacga gcggcttacc caacttaacc aacagcagcg ctgtgacgcg
     2581 tttattcagt ttgccagcga tgttatgttg acgcgctgtg agctgaccag cgaggcgaag
     2641 gcggcagaag ctgcaatacg tctgtgtaaa gagctgggag atcagtcggt cgtgattagc
     2701 ggtgacacga ggctggagga attggggatt agtgaacgtt tgcagcagga atgcaatgcc
     2761 gttgtttggg atccggcgaa aggtgccgag aatatctcgc aggcagagca ggctaaagtg
     2821 ggtgtcgtgt atgctgaata tggtttaacc gaatcgggag gcgtggttct tttttccgcc
     2881 gtcgagcgcg ggcgttcatt gagcctgctc ccggaatctt ctctttttat cctgcgtaaa
     2941 agcacaatcc tgccgcgtgt agcgcaactc gcagaaaaat tgcatcagaa agcgcaggcc
     3001 ggtgaacgaa tgccgtcatg tattaacatc atcagtggtc ccagctcaac ggcggatatt
     3061 gagcttatca aagtcgtcgg ggttcatggt ccggtgaaag cggtgtatct gattattgag
     3121 gattgctgag agagatatga gatctgaaca taaacctgaa gaaggcatac gaggttgaaa
     3181 atagcgttaa ggacaacctg acttaacccc gttccgtcat ggttatgct
//
LOCUS       DAZOYL010000351         3207 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZOYL010000351 DAZOYL010000000
VERSION     DAZOYL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.352"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            169..1851
                     /locus_tag="ACHUOY_003797"
     CDS             169..1851
                     /locus_tag="ACHUOY_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR0684438.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1903..3060
                     /locus_tag="ACHUOY_003798"
     CDS             1903..3060
                     /locus_tag="ACHUOY_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0684439.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3026..3148)
                     /locus_tag="ACHUOY_003799"
     CDS             complement(3026..3148)
                     /locus_tag="ACHUOY_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684440.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          977 a          685 c          639 g          906 t
ORIGIN      
        1 gagaaattac ttaatagaac agaaagtaat gtcaacgctt tatggactgt tatttccctt
       61 tttttagcta aatctgctat ctctttatgt gactaacttc acttacatcc acttatttct
      121 cttcgtaaaa ttacttcgga attaagtaca ataagaagag gaacatttat gaagtctgca
      181 ttaaagaaaa gtgtcgtaag tacctcgata tctttgatac tggcatctgg tatggctgca
      241 tttgctgctc atgcggcaga tgatgtaaag ctgaaagcaa ccaaaacaaa cgttgctttc
      301 tcagatttta cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc
      361 atggatgatc ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca
      421 atggaaaatc gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct
      481 gcacaaaaat caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac
      541 ggctatgtgg cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct
      601 cccgcccgct ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa
      661 actttcttgc ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg
      721 cacttgtcaa aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat
      781 gacaacttca ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac
      841 tttatgggat tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat
      901 cgtgaacgtg tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc
      961 gttgttgatc gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct
     1021 ccgcacctac caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt
     1081 agtcaaacag cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc
     1141 attctcgaac aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc
     1201 gataatggtg cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag
     1261 agtcagacct atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt
     1321 caacccggta attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat
     1381 gcagccgata tcagcattcc aaaagacctt aagctggatg gtgtttcctt gctgccctgg
     1441 ttgcaagata agaaacaagg cgcgccacat aaaaatctga cctggataac ctcttattct
     1501 cactggtttg atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat
     1561 cagtcagacg attacccgca taaccccaac actgaggact taagccaatt ctcttatacg
     1621 gtgagaaata acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac
     1681 aaactgacgg atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa
     1741 gagatgcaag gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta
     1801 aatcaggaga agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt
     1861 catgcggcgg atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag
     1921 ccctccagtt ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg
     1981 cagtttactc atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat
     2041 atcgaagcgt ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct
     2101 ggcctggatt ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt
     2161 atttttaatg cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc
     2221 aaagaacatg aatttctggt tggtatctcg atcgatggcc ccctggagtt acatgaccgt
     2281 tacagacgca gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt
     2341 ctgaaatcat atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat
     2401 taccctcttg aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc
     2461 gaattgctag aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc
     2521 cgtatcattg atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt
     2581 atgcaatggg ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc
     2641 agccgttttt tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat
     2701 ctggttgttg aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac
     2761 aaaattggaa acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg
     2821 caaaaaaaac ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc
     2881 ggttgtccta agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa
     2941 ggttataaaa tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct
     3001 aagaacagag taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa
     3061 taacgctctc ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga
     3121 cataaattcc tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac
     3181 gtggtatgcg tcagggcatt agcaata
//
LOCUS       DAZOYL010000352         3203 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZOYL010000352 DAZOYL010000000
VERSION     DAZOYL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.353"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(67..828)
                     /gene="kduD"
                     /locus_tag="ACHUOY_003800"
     CDS             complement(67..828)
                     /gene="kduD"
                     /locus_tag="ACHUOY_003800"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR0684441.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(893..1729)
                     /gene="kduI"
                     /locus_tag="ACHUOY_003801"
     CDS             complement(893..1729)
                     /gene="kduI"
                     /locus_tag="ACHUOY_003801"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR0684442.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2016..3197)
                     /locus_tag="ACHUOY_003802"
     CDS             complement(2016..3197)
                     /locus_tag="ACHUOY_003802"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR0684443.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          843 a          874 c          727 g          759 t
ORIGIN      
        1 ttatgatgac ctacaggtat aaaaaaccct gccatgaggc agggtcataa aagtaagagg
       61 aatgaattaa cgagccagcc aaccgccatc tacggcaatg gtataaccat tcacataatc
      121 tgaagcgctg gaggcaagga acactatcgg ccccatcagg tcactcggca gtccccaacg
      181 accagctgga atgcggtcga gaatttccgc gctacgttgt tcatctgccc gtagttgttg
      241 agtattgttg gtcgccatgt aacccggagc tatcgcatta acattaatgt tgtgtttagc
      301 ccattcgttc gccatcaatc gcgtcacgcc catcacgccg ctttttgatg cggtataaga
      361 aggcacacgg atcccgccct ggaaggagag cattgacgtg atattgataa tcttgccgcc
      421 attgcgttgc gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat
      481 attcaggttc atgacatcgt cccagtcttt ttcgctgaac tcgagagcat cttcgcggcg
      541 aatcaatccg gcgttattca ccaggatatc aatatgacca aactcagcta ccgcgcgatc
      601 cagcagtgct ggaatgccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg
      661 ccccagcgcc gtgacctgct tgatggtttc agtcggttca acgatgttaa tgccaacaat
      721 gtcacagccc gcttgcgcca gccccaacgc catcccctgg cccagtccag tatcacaacc
      781 agtgacgacc gcaactttac cttcgagaga aaatgcactt aaaatcataa caatacctta
      841 ttcgtttatg ccgacaacta gcgcaaatct ttaacggcca ctatgccgac aactagcgca
      901 aatctttaac ggccacatgg tccatatcat caaagacctg gttttcacca accatgcccc
      961 agataaaggt ataagctttg gttccgacgc cggaatggat cgaccagctg ggggagatca
     1021 ccgcctgctc gttatgcatc acaatgtgac gcgtttcttg cggctgcccc atcatgtgga
     1081 aaacgcaggc gtcatcatcc atattgaaat agaaataaac ttccatccgg cgctcgtgag
     1141 tgtgacacgg catggtgttc cacaagttac ctggagccag ctccgtcagc cccatactca
     1201 attggcaggt ttccagcaca tccgggacaa aatatttgtt aattgtgcga cggttactgg
     1261 tgaggttatc gcctaacgtg actggagata cttcgtccgg tgtgactttt ttggtgggat
     1321 acgtcgtatg cgcgggtgcg caattgtaat aaaactttgc cggagtggcg gtatcaatac
     1381 tggcaaagac aacttctttt gcacctttac caacatacag ggcgtcgcgg tgaccgattt
     1441 catagcattg gccatcgaca gtaatcgtac cggcaccgcc aatattgata acacctaact
     1501 cgcgacgttc aaggaaatag cttacgccga gttgtttacc aacttccccg ccaacggaaa
     1561 ccgttttagt tatcggcata atgccgccaa caataattcg gtcaatgtgg ctgtaaacca
     1621 tggtgtactc atcggcgaca aatacctttt caaccaaaaa ttcattgcgc agcccttggg
     1681 tatccagcgt ttttgcgtgc gcactgtgga tgctctgtct tacgtccaca tcaacctccg
     1741 ataatcagtg aagcgtgaaa atagaaaacg atagtgaaac actgtttcgt tttctctata
     1801 aagggatgat atgcccatgg gctagggttt tcaattaaaa ataaaacaat gtttcatttt
     1861 taagttaagg attaaaaaaa gtgcagtgtc gatcacgaat atgtcatgag aatggaaaaa
     1921 tgagggagcc cagaaaatgt atgtcgactt gccagccatc attattcaac agattaatga
     1981 cggcagatat ttatcggtaa ggaggaaatg cagagctact cgtcacgttc aatggtcaat
     2041 gccacgccct ggcccccgcc gatacaaagc gttgccagcc ctttgcgggc attacgtttc
     2101 accatttcat gaaccagaga aaccaggatt cggcaaccgg aagcgcctat cgggtgaccg
     2161 agtgcgatcg cgccaccatt gacattgacc cgacgctcat cccactcaag catcttgcca
     2221 accgaaagcg cctgtgcagc aaacgcttca ttagcctcga taagatcgac atcagccaac
     2281 tgccagccta cacgctccag gcaacggcgg gtcgcataca ccggcgcaat tcccatcaat
     2341 gccggatcta caccaacgct ggcaaatgcg cggatgcggg ccagcacggg taaattcaac
     2401 gctcgtgctt tggcttcgct catcatcatt accgcagctg cgccatcgtt tatggatgat
     2461 gcattccccg cggtcacaga accgagacta tcaaatgaag gatttaaacg ggctaaacct
     2521 tctgcactgg cgtcagtgcg tggctgttca tcggtatcaa caaccaacgt ctgaccgtta
     2581 ctttggatca ttaccgggac gatctcatct ttaaatcgtc cggcgtcaat cgccgctcgc
     2641 gctttttgtt gcgagctaag tgcgtaagca tcctgcaact gacggctgat gccatattcg
     2701 cgagccagat tttcggcggt gacaccaata tgataatcat tgaaggcatc ccacaaccca
     2761 tcatgcacca gactgtcaac caactggcta ttgccaagct gtgcaccggt gcggctatca
     2821 gtcagaacat gtggtgcgcg gctcatgttt tcctggccac cggcgatgac aatatcagcc
     2881 tcgccacact gtatcgcctg agtagccaga tgcagtgctt taagtccgga accgcaaacg
     2941 tcattaatcg tgattgcaga aacgctatta ggaagaccac ctttaatagc cgattgcctt
     3001 gccggattct gccctgcccc tgcagtcaac acctgaccaa gaattacttc atccaccgca
     3061 tatgcaggaa cgccggtacg ttctattaac gctttcacga ccagactacc aagttccacg
     3121 gcggaatgac ccgctaacgc accacgaaag cagccgatag gtgtccgtaa cgccccgaca
     3181 atcacaacgt ctttcatcac aac
//
LOCUS       DAZOYL010000353         3183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZOYL010000353 DAZOYL010000000
VERSION     DAZOYL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.354"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUOY_003803"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUOY_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR0684444.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUOY_003804"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUOY_003804"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR0684445.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="ACHUOY_003805"
     CDS             complement(1715..1984)
                     /locus_tag="ACHUOY_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR0684446.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUOY_003806"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUOY_003806"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR0684447.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUOY_003807"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUOY_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR0684448.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          771 c          769 g          811 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gca
//
LOCUS       DAZOYL010000354         3182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZOYL010000354 DAZOYL010000000
VERSION     DAZOYL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.355"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(16..2904)
                     /locus_tag="ACHUOY_003808"
     CDS             complement(16..2904)
                     /locus_tag="ACHUOY_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0684449.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT          866 a          717 c          748 g          851 t
ORIGIN      
        1 tcggagtgaa gaatattacc aggtatattt aacacccacg ttcgcggacc agtcttgatc
       61 tacgtcacca ccaccgaggt agttagcatc ggtataggcg ctgaagttct tggtgaagct
      121 gaactgagta cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc
      181 accgttcaca tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg
      241 agtcagagcc tgatcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata
      301 acgcatgctg tcgtaagact gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc
      361 accagactgg aacagaccag aaacgctgcc gtaaggagtc acgtaaccag catcacccag
      421 tttgaagtcg taaccggctt tcaagccgaa gccccaagcg tcggagttgg tgctaccgtc
      481 aacgtaagta ccgttgctca tggttgcaga caggtcgttg ttgaagtgag agtagctcaa
      541 gctaccatca acaaagacgt tgttcgcgaa gtgagcagaa gagtagatgt aggcagtctg
      601 gctgtcttga tctacctgac cagaacggtc attcatgtca cctttagcga agcctgcagc
      661 cgcaccgacg atccacttag cgttgttacc gtcaactttg gtatcaacac cgaccatgat
      721 gccgttaaca tcctgatcat agttgatggt gccgttgtcg ccgttgaagt taccaccgaa
      781 gtagcttacc catgcgccgc cgttatcagc caggccatga cgagagttgg tcagacgagt
      841 accaacggtg tcttgttcca ggttccagat attggtgttc gcagacggga tgctcagcgc
      901 catgttagcg tagtcggtca gctccatctg ttgcagaaca acggtgttac cgcgctgttc
      961 agcctgatag gtgtatgcac ccaggtcagc tttgttagca gcagagaagg tcgcgttggt
     1021 tttggtatca tcgatataga tgagctgctt acctgcgtaa tcagcaatcg caccagcgcc
     1081 agtggagttg tcgatacgaa ctttgtagtt accggttgca gctacaacgt tgtctgcaga
     1141 gctgttgtca acttcgtcgc cattgttaat gtcgttgtta ccattaacgg tcaagtgacc
     1201 atcagagttc atcgcaataa caccataacc gtagttagct ttgctggtgt cgttggtacg
     1261 atcgttgacg aggtctgcat tcaggatgta atcattacta tgaatgtcaa acacacctgc
     1321 gtaaacatta ccgttgttat ctttaacgtt gccactggtc agttccagag tatcagttgc
     1381 aaatacgctg gagatgtcat tcatgttggt gtttacatcc agcacaccac cgttagtaac
     1441 ggtaatggta ttggcataca gcggtgcata tgcccaagct gaagcagtat cttcaccgaa
     1501 gttaacggtt gagaaggtat caatggtcag gtgatcggtg ttaacttcac cgttaacttc
     1561 cattgaagaa ccaccgttca gagtgatggt gtcagaagtc agagaagaat ctgcactcac
     1621 gttaacctga gaaccgctgt tgatcgccag ggtatcgatc agggaagttt tcgtggtttc
     1681 ccaatcagaa ccgttgttca gaacaacatc gaatacgccg ctctggaatg cgttagcgtt
     1741 atcaacacga ttccagccat cttcagagta agaagcgtac gggttcaggc tgtttggtgc
     1801 aacgtcgtag aaggcttcgt cagcaacaac aacgtcataa tcaatattcg ctgaaccaac
     1861 ccatttggtg ttgttcagag tcaggttcag gtgatcgacg ttctgatcgt cagttgccca
     1921 accgccatga gtgtaagcag tggtagagtt gtcaatcagg tgacccgtcg cgtcaaagtt
     1981 agcgttccag gtactgtcaa atacaacgtc accaagcaac tgagagttgt tgaacgtaac
     2041 gttgttgacc atacgataat cagaagtgtc atcatcgatg aaggctaacg caacatcatc
     2101 agcaggagat gcgccattgc cgtagtcact tggttcaaca gaattgccga aatgaccacg
     2161 atctgattgt tcgtcttcag tccatgaacc agaagttaca gtagagtcat taacaacgat
     2221 gttgttgtcc cactgatgac cttcggtatt gtaggtcgca ttaccgaggt cgtgttgaac
     2281 caaagtaata cctgcaacat gggagttgtt gctgatgttg atgttacttt caacatccag
     2341 agtcacagcg gtaccaagac cagcggttac caaacgatca taatctgtct tagatgtttt
     2401 accgtctgca tccagataag tatcggtgaa gtagaaggcg tcaaaatcat cgtcaatagt
     2461 agaattagcg atgttcagag tgaagatgtc gccatcaacc cagtcgtggc cagtataatc
     2521 gctaccgttg gtccagacgt agtcgccata accaacctgg taagatgtaa ccataccatg
     2581 aacaattgag ttggagatgt tcagtgtatt accggtatct gtgccataat cgtggttcag
     2641 ataataagta gaaattacac catttacagt gtcgccgctg aaagtggtat caaagtaacc
     2701 atcagctgcg tggtcatata cataaccacc gtaagtcaga taatcgtgat caattttagt
     2761 gctgtcaccc caattgtaga tggtggctgc gtcatcgtga tacccgaagg tgttataagt
     2821 agctgcatta gcctgagagg tgatagccag agtgcaagcc aatgctaatt gtgatactac
     2881 aagtttcttt ttccaggagt gcatttgtca tccctcctca gggacgtaat aagttgatcc
     2941 atcaattatc aatgcataga aaacatatta tgtttcgcgc taacgattat gctgcgttac
     3001 agacgaatgg ttcaattatt ctttccccta agtccgaatg tggacatata taatttatgg
     3061 agaaaaacac gcatcaaatt gataaaagga cgatatttca acatgttgca gataatgtta
     3121 tttttgatac atacgcgtta taaatatcaa aaacacaaac cagataaata tcttatcaat
     3181 at
//
LOCUS       DAZOYL010000355         3176 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZOYL010000355 DAZOYL010000000
VERSION     DAZOYL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.356"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            534..2387
                     /locus_tag="ACHUOY_003809"
     CDS             534..2387
                     /locus_tag="ACHUOY_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR0684450.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2538..2753
                     /locus_tag="ACHUOY_003810"
     CDS             2538..2753
                     /locus_tag="ACHUOY_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0684451.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2758..3072
                     /locus_tag="ACHUOY_003811"
     CDS             2758..3072
                     /locus_tag="ACHUOY_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR0684452.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          778 a          763 c          885 g          750 t
ORIGIN      
        1 ccttccaggc ccatccgctc cggtgggcct ttttactgca gaaaacaggt tccccgttaa
       61 atgctatgtt gctcacaatt cagtaagttg acagttgcct gtcagactgg gcatttgtta
      121 aaaaaatttc gcatggtgaa tccccctgag cggaggggcg actggtgacg gtataatctc
      181 tgattatcaa aacgagaatg acgcgggttt agtggcaccg ggctgaactc accgggaggc
      241 acccggcacc atgtgcatga tgatacagat acgcggcttt agcccctctc cggaggggtt
      301 ttcttgtggg caaaaaaagc ccgcgctggg agacgcgggc ggcaaggaat aaacaataaa
      361 acgtgaagta atatttcagc tggcgaataa taccccatag taatcactct gcgcaactgc
      421 gcggtctttt tcgaattgcg ggctgtcgtc tctcttctgc cattgtcctg taacttccgg
      481 acttcagccc gctccttatt ttactcacaa tattatcccg gccgggagga ttcatggcat
      541 ttaaacacta tgacgtggtc agggcggcat cgccgtcaga ccttgcgaaa cgactgacac
      601 aaaaactgaa ggagggctgg cagccgtttg gtagtccggt ggccataacc ccttataccc
      661 tgatgcaggc gattgcagca gaaggtgatg tggtcgtcag tggtgcaact gagccggagt
      721 ggtactacgt catcgtactg gccgggcaat ccaatgccat ggcttacggt gaaggtcttc
      781 cgcttccgga ttcatacgat gcgccccatc cgcgcattaa gcaactggcc cgtcgtaaca
      841 cagtgactcc cggtggtgaa gtatgcgtat ttaacgacat cattcctgct gaccattgtc
      901 tgcatgatgt tcaggatatg agtacgatta accatccccg ggctgacctg agcaaagggc
      961 agtacggctg tgtcggacag ggcttacata ttgccaaaaa actgcttccg tatatcccta
     1021 ataatgcggg gatcctgctg gtaccatgct gtcgtggtgg ttcggcattc acccagggca
     1081 cggaggggac attcagcgag tccacggggg ccagtcagga ttcggctcgc tggggagtgg
     1141 gtaagccgtt atatcaggat ctgcttttcc gcacgaaggc agcattgcag aaaaacccga
     1201 aaaacgtttt gctggcgata tgctggatgc agggggaatt cgatatgacg aatgccagtt
     1261 acgcccagca gccagcagca tttcttgcaa tggtacagca gttccgtgct gaccttgccg
     1321 ggctggcggc gcagtgtcac ggtggaagtc cggcatcagt cccctggatt tgtggcgaca
     1381 cgacatacgc gtggaaacaa gaacacggta cgcaatatga agtggtatat ggtgcatata
     1441 aaggtaaaga atcccagcag atttattttg ttccctttat gaccgatggt agcggagtta
     1501 atacaccgac aaacaacccg tcagaagatc ctgatattgc cgggtctggt tattacggtt
     1561 cggcatcccg aacgaacaaa aactgggtat catcaaatcg cccgacgcat ttcagctcat
     1621 gggcgcgtcg tggcattatt cccgatcgta tggcaactgc tattctgaac gtagccggtc
     1681 gcaccttagc cttcattagt ggtaaggcac cggaaatcaa accctcgccc ggcggcgaca
     1741 cgccatcggg gccgtctgaa gatgcatcca tacgcacaat ctccctgttg ccgacagccg
     1801 gagacgctgc tgcgcagggc tggagcatta agaatggcgg agttcagttg tcagatggtg
     1861 tatttaagat caccaagcag agcaataaag cctggtccct gacgcgcccg gtggatgacg
     1921 cagtctccct gctgacacgg ggtggcagac tgagctgtaa gtttcgactg tcaggcgcac
     1981 tgaccaacaa ccagttcggt ctgggaattt atctgtatac cgatgtagcg ttacctgacg
     2041 tcgtggcgat gaccgggact ggtaacccgt tcctgatgtc gttcttcacc cagaccacag
     2101 acggcaaact gaatctgatg catcacaaga aagcaggaaa cacaaagttg ggcgagttcg
     2161 ggaattacag taacgactgg ctgacgctgg agctggtgtt caccgccggc agtgccacgg
     2221 ttactccgaa actgaatgga gtggctggcc cggcattcca ggtcataaaa gacagtctga
     2281 cactggggct gaatgcgctg acgctgacgg atattaccaa aaatgcagcg tatggcgttg
     2341 agatagaaag tctggtgctg gagataaatg caccggcatc atcataaaaa gtgagccagc
     2401 caaatggaag gtatcgttaa actcaccggt agtgtcagtg ggtcgtctga gatgcctgca
     2461 tgagttatca gagccatcag tacttaactg gtggcttttt ttattgttgt cagcttccgg
     2521 ataacgggag acggggtatg taccagatgg aaaaaatcac aacaggtgtg tcatacacca
     2581 cgtcagcggt gggaacgggc tactggttcc tgcagttgct ggacagggtt tccccgtctc
     2641 agtgggcggc aataggcgtg ctggggagtc tgctgtttgg gctgctgaca tatctgacta
     2701 acctgtattt caaaatcaga gaggaccgtc gtaaggctgc acggggagag taattcaatg
     2761 actcaaaact atgaactgat tgtgaaaggg atccgcaatt ttgagaataa agttacggta
     2821 actttagcgt tacgggacaa aaaacgcttt gacggtgaaa tttttgacct ggacatctcg
     2881 ctggaccgtg ttgaaggtgc cgcgctggag ttttatgagg cagcagccag aaggagcatc
     2941 agacaggtct tcctggatgt tgctgccggg ttatgtgaag gggacgagct gttgccagaa
     3001 acgcgcccct gttcagaggc gcggtatacc ataaaaatta acagttctga taactcgatt
     3061 acgggttgtt agctttttgc agttggcttt ccagtatctt tcattggtag catcctgata
     3121 aatatccatg agcgcaaaaa tcaaatacgg cctgtcagct gctgttctgg cgctga
//
LOCUS       DAZOYL010000356         3159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZOYL010000356 DAZOYL010000000
VERSION     DAZOYL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.357"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1199)
                     /locus_tag="ACHUOY_003812"
     CDS             complement(<1..1199)
                     /locus_tag="ACHUOY_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR0684453.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="ACHUOY_003813"
     tRNA            1492..1586
                     /locus_tag="ACHUOY_003813"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1858..3070
                     /locus_tag="ACHUOY_003814"
                     /pseudo
     CDS             1858..3070
                     /locus_tag="ACHUOY_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          914 a          743 c          716 g          786 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa ttctgcatga acaaggcttt aattccgcct ggattgaaat
     2941 gcagttagct catgtggata aaaattccat caggggtacc tataatcatg ccctgtatct
     3001 cgatggccgc cgtgaaatga tgcaatggta cgctgattac attgattcac tctccagccg
     3061 ggaaagttaa gtcagaaata cttttccctg atattttcct caattaagta ttttatcctc
     3121 tggcctacaa ggctggagga tttttgttat tatattatt
//
LOCUS       DAZOYL010000357         3138 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZOYL010000357 DAZOYL010000000
VERSION     DAZOYL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.359"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHUOY_003815"
     CDS             <1..246
                     /locus_tag="ACHUOY_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0684454.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(247..1677)
                     /locus_tag="ACHUOY_003816"
                     /pseudo
     CDS             complement(247..1677)
                     /locus_tag="ACHUOY_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            complement(1887..3035)
                     /locus_tag="ACHUOY_003817"
     CDS             complement(1887..3035)
                     /locus_tag="ACHUOY_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0684455.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          958 a          815 c          698 g          667 t
ORIGIN      
        1 gatgttgatg ctgaactgga tgccgttttg atgggtgctg gtatcgggta tctgctctat
       61 gaacagatca aggagtacct tgataccggt cgtttagaat gtgtactgga agactggatc
      121 acagagcgtc caggcttcca gatctattac ccaaaccgcc agtatatgtc ctgcggcttg
      181 cgcgcatttc tcgattatgt aaaaacgggt cagatctgcc agagtcagcg tcaccgacca
      241 caataattat ttactattcg ctttacgcgc cagcagcggg aatatcagtc ctaatcctac
      301 cagcacaaac ggcgttgcaa cattcagcgc caactggaag gtccactctg cagtgaaggc
      361 ttccattttc gggaagatcc ccgtcaaaca ggcaaaggcg gtaaaggcaa aacaccaaat
      421 cccgacggtc attgccagag gcttattacg aataaagaca tattctggtt tatatttctg
      481 cgccaggcga acgactgcaa taaatgcaac aaatacccat aaataacgca gcggcatcac
      541 taccgaatta aggttcaaca gccatttata gagattgttc atatcaccaa tgccgagagt
      601 cggcagcata atcagaatcg ccaccagtac cagggtcaga aaatagccat taacgggcgt
      661 accagaagcg ttggtacgac ataaactggc tggaatatat ttgctgtcag catcacctaa
      721 tagcacttta agcggggcat caatcgagaa taccagcgcc gctacttgtc ccagggtatt
      781 cgcaatggcg taaatcacca ttaaagtatt acccatgttg taatactcgc ccagcttctg
      841 aaaggcgtaa tactgaccgt tggtcattaa gtcatccggg atattacgcg aatcaaacat
      901 catccccatc gccagcgagc ccagaatggc acaaaccgca accatcaccg ccaggcataa
      961 catccctttt ggaaattctt ttcctgggtt gcgcgtttga ttaacgtaag gagaaatctt
     1021 ctctgctccg ccaaccgcga aaaccagcat tgaaatagtg gtaatgtagg taaagtcgat
     1081 atgaggaatg aacgtttccc aggtaatgtt tgtggtcgca atatgcactt cagtaattgc
     1141 aggcgcggtt accgccatcg ccacatacag gagcgacata acaaacatag caatccctgc
     1201 cacagaaccg acgattttca gcgatttcat accgcgtgaa gcaacccaca taaagaagat
     1261 aaacagcacc agcgttaacc cctgtaacgc tacgactgaa tattctttga ttagcgaacc
     1321 gtcgcctttc atcgcccaac cgagcgcaat cagaattgcc tagggttttt gtgccagata
     1381 gggaatatgc accacccagt aggtccacgc ggcgagataa gccagtccgg gtcccatcgt
     1441 gtgtttaatc caggtactga ccccgccctt cccatctttg aaggtcgagc ctaactgacc
     1501 aacaattagc gcataaggtg tgaaatagag cgcaaagata aacacccatg aaaaaacaac
     1561 caccagcccc tggttggcgt agttattaac aacgttgcca aaaccccaga cagtgataaa
     1621 agacatcagc gcaatgttgt accatcgcag ctgtttttcc tgaacattcc cagccataga
     1681 caaatccttc gccaaaatta taggggcatc tgtaaaacga gggattatgc gcggcaaata
     1741 agaagtgcgg aaatagcgaa atgcaaaatt ataacctgct acacatttta tttttaaacg
     1801 aataaaattt ggaataaagc aagtcagata gaagaaacaa aaaagcagag tgtgaaacag
     1861 taagttcaca ctctgcttga attcatttac acgtgagcaa cgggtttcgg cgtatgaccg
     1921 gcgttgcgca gcagcatcag caaatagata aacgatacgc tggcgatcat gataaacaat
     1981 aaattatcgg agaaattctg cattagcata gcggtaaatg acgggccaag cagacttccc
     2041 acagtatagc tcaacagtaa ggcttggttc atcgccacca gttgatgatg ttcaactttc
     2101 tcgcaagccc atgccatcgc caccggatat agcgtaaagc cagcggcacc gaggatgaat
     2161 aacgctgggg ccatcgccgc ctggctaagc atcgcaatac tgccgagaat gacgacaaag
     2221 acctgaacac gcaaaaccag cagtcgacca aacttatccg ccagacgtcc aatcggccat
     2281 tgtccgagga tacccgcact gaccagtacc gccatccaga aaccaatact ggcattgctc
     2341 acccctttgt gattgaggta cagaggcatc aggccatata gagatcccag aacgattcct
     2401 gagataatgc agccattcac gccaagccgc gcctgacgga gttttagcat tgccgtaatt
     2461 gacgtcgaat catggttttc agcctgctga ttcaggacac gcgtaaacaa cagcggtaag
     2521 atccctgcca atgtcaaacc tgtaacccac ggcaatacgc tcatcagctc ggttgagacc
     2581 ttgctgacca gtaactggcc taaaaacgtt cccacgtaat aaaccatcat gtatgcagca
     2641 agcaaacgcc cacggttacg tgacgtccca ctgcacatca gcgcgctctc aacgactacc
     2701 caaatcatgg cacagccgac gcccgcgaca aaacgccacg ccaaccaact ccagaatcca
     2761 atcatcaggc caaggccggc acagccagcg gcaaaaatga aggaggccag ataatagctg
     2821 cggttaaaac caatgcgttt aatgacatac cctgtcagca atgtaccgac aaggttgccg
     2881 gtaaaatagg atgagctgac aacgcctacc tgccatgtgg acatgtgttc ctgggcgagc
     2941 caaagcggca cgagtgtatt taacaccgcg atcgccagag tcaacaaaag caggccagac
     3001 agcaaaagca tgacaggccg ggtatacgtg gacatgaatt aaaaccgtga ggaagttcaa
     3061 atttcatgcg catcatgcca ccgccaaaaa cattgtcaat gggtgaaaat acgcgcatat
     3121 cgcaacggcg gtattatc
//
LOCUS       DAZOYL010000358         3076 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZOYL010000358 DAZOYL010000000
VERSION     DAZOYL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.360"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            113..445
                     /gene="hdeA"
                     /locus_tag="ACHUOY_003818"
     CDS             113..445
                     /gene="hdeA"
                     /locus_tag="ACHUOY_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR0684456.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            561..887
                     /gene="hdeB"
                     /locus_tag="ACHUOY_003819"
     CDS             561..887
                     /gene="hdeB"
                     /locus_tag="ACHUOY_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR0684457.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            951..1598
                     /locus_tag="ACHUOY_003820"
     CDS             951..1598
                     /locus_tag="ACHUOY_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR0684458.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1640..2170)
                     /locus_tag="ACHUOY_003821"
     CDS             complement(1640..2170)
                     /locus_tag="ACHUOY_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0684459.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2326..2892)
                     /gene="slp"
                     /locus_tag="ACHUOY_003822"
     CDS             complement(2326..2892)
                     /gene="slp"
                     /locus_tag="ACHUOY_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR0684460.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          896 a          608 c          672 g          900 t
ORIGIN      
        1 atctgatttt gatattttat atcaacatga catatacaga aaaccaggtt ataacctcag
       61 tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
      121 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
      181 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactcc tggacctgtg aagatttcct
      241 ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
      301 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
      361 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
      421 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
      481 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
      541 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
      601 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gctggcagcc aatgaatccg
      661 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
      721 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
      781 aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
      841 atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta
      901 aaaaggagta gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag
      961 aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc
     1021 aaatgcgcgg caaaggagca gggttacgca cacatgtatt aattggcatg ggaagcgccc
     1081 tgtttatgat tgtttcgaaa tatggttttg ctgacgtgct gtctttagat cacgtcggac
     1141 tcgaccccag ccgtattgct gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta
     1201 acattctcgt tcgtaaccaa aatattgtag gtctgacgac ggcagcggat atctgggtga
     1261 ccgccgccat aggtatggtt attggcagcg gtatgtacga actgggtatt tatggttcag
     1321 tgatgacctt gttggtgctg gaagtcttcc atcaattaac cttccgcctg atgaacaaaa
     1381 attaccattt acagctgaca ttagtgaacg gtaatatggt atcaatgctc gactggttta
     1441 agcagcaaaa aataaaaact gatttagttt cgttgcagga aaatgaagat catgaagtgg
     1501 tcgccattga tatccagctt catgccacaa cgtcgataga agacctgttg cgattattaa
     1561 aaggtatggc aggggtgaaa ggcgtttcaa ttagctaatc tgtgtggcag cgtagccaga
     1621 ctcaccgtaa gcctgaaatt cacaccagat aatcaatatg ctgatggcgt accagctcat
     1681 tgatccgctt aagattgagg atcatcatga tattgtgttt atggcagtag aacgttttta
     1741 gcgatatttt cagtttctcc agaatatctt cagttgtcat ttgattcgca atgcagctca
     1801 gaacctgatg ttgcttgcgc gaaagtgaca cattcgcttc aggcacagag cagtaactgc
     1861 cattgatgag tgcgacaaaa tcaatgatag tgatagtccg cggaacaaag gtcaccggta
     1921 ctttccccag acagcgttta atattaaagg gggagaaaac aatgacatgg acaggtttta
     1981 atcgttcaat ttgagtgaga aaattagaat gaaatacatt attcattaat gtatcaataa
     2041 tgacgaaggc attctgatgc aacattacgt cgatctcttc ttcgttctgt atatgaacga
     2101 cattaccttt actcagaatg tttttcatcg cggtgaagaa catcgtatcc ctggtaatta
     2161 taagaaacat aaccgactcc tggtgcggac attaacttta taatcaattt gccaaaggta
     2221 attaacagga cagggcttat aataataata gttagcttac tgtatattaa ttttatgtaa
     2281 tacatgatgc agatgctgag aaagcatctg catctttcgg tggtgttatt tgaccagctc
     2341 aggtgttacc tgactcaccg cattggtgta gtaaggcgca ccccagcccg gttccggcca
     2401 gaatgcacca tagccgtaat cccacaggtt ataggtggta ttaaccactt ctctcaaatg
     2461 ccacacctgg atgccctgca tattcacttc caggaagtta tacgggactt tattgataaa
     2521 gccaggttgt tcaccctgaa tggtaccgag gatggtaaca aagtgattac gatagttcac
     2581 tgggtcaagg aagccgcttt gtctggcgag cagtcggccc tgatagttgg cttcaatatc
     2641 aggtttcgca tagctatcca acggtaatac agcgatttct aacaacgtat ccgttttgcc
     2701 attgataacg ttgataacct tcccaccaaa gcgcgcttgt tgaccaacat ataaccccgg
     2761 ctggttatga acagcaacaa aacttttttg aatatcaggt tggttattgc ctttgatatt
     2821 ttgcggaatt gaactacatg cggcaagcaa aaatgaaagg ctgaggatga gtgcaccttt
     2881 tgtcatgttc atgttactat ccttatcaac aaatattaaa accataatag atatttcttt
     2941 atagttttca tctgattctg aggcgatatg cctgatagta acgtaaacct gtctgatgtt
     3001 tatatccctt ttgtttagtt caattccatt gattttgggg cgtggcatga gtatccatcg
     3061 ccccataata taatcc
//
LOCUS       DAZOYL010000359         3017 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZOYL010000359 DAZOYL010000000
VERSION     DAZOYL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.361"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            203..2482
                     /gene="ydeP"
                     /locus_tag="ACHUOY_003823"
     CDS             203..2482
                     /gene="ydeP"
                     /locus_tag="ACHUOY_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR0684461.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2730..2927
                     /gene="safA"
                     /locus_tag="ACHUOY_003824"
     CDS             2730..2927
                     /gene="safA"
                     /locus_tag="ACHUOY_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR0684462.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          834 a          675 c          714 g          794 t
ORIGIN      
        1 gaaaaatatg ttaatttaaa cactatgaga gatgatgctt aacttatcgt atttaatcta
       61 ttgtttaacg ataatagatt aaaaatagaa agagagtagt cccctactct tatatatcca
      121 tgttggcgat aatccctttg tatgtactgt gcatcatcgc tattacaaat cctaataatt
      181 catttccaca caggataagt agatgaagaa aaaaattgaa tcctaccagg gtgctgcagg
      241 tggttggggt gctgttaaat ccgtagcgaa tgcagtacgt aagcagatgg atatacgcca
      301 tgatgttatt gccatgtttg acatgaataa gccagagggc tttgactgtc cgggttgtgc
      361 atggccagat cctaagcaca gtgcgtcatt cgacatttgt gaaaacggcg caaaagcaat
      421 cgcctgggaa gtcacggata agcaggtaaa tgcctctttc tttgctgaga atacggttca
      481 atcattactt acctggggag atcacgagct tgaggctgcg gggcgtctca ctcagccttt
      541 gaaatatgat gccgtcagcg actgttacaa gccattaagc tggcaacaag ctttcgacga
      601 aattggcgca cgccttcaaa gctatagtga tcccaatcag gttgaattct atacttcggg
      661 ccgcacttcc aatgaagctg cctttcttta tcagcttttt gcccgtgaat acgggagcaa
      721 taactttccc gactgctcca acatgtgcca tgaaccgaca agcgtgggtt tggcagcgag
      781 tatcggtgta ggtaaaggga ccgtgttgct ggaagacttt gagaagtgcg atttagtcat
      841 ttgcattggg cataaccctg gtacaaacca ccctcgcatg ctgacttcgt tgcgcgcttt
      901 agtgaaacgg ggagcgaaaa tgatcgccat caatcctcta caggaacgtg gcctggagcg
      961 atttaccgca ccgcaaaacc cgtttgaaat gctgacgaac tctgagactc agttggccag
     1021 tgcctactac aacgtgcgca ttggtggcga tatggcgttg ctcaagggga tgatgcgcct
     1081 gttaattgag cgcgatgatg ctgcaagtgc cgcaggtcgg ccctcattgc ttgatgacga
     1141 atttattcaa acgcataccg tcggctttga cgagctacgc cgtgacgttc tcaattccga
     1201 gtggaaagat atcgaacgta tttctggact aagtcagaca caaatcgccg aactggctga
     1261 cgcatatgcc gctgccgatc gcaccattat ctgttacgga atggggatca ctcagcacga
     1321 acatggtacc cagaatgtac agcaactggt caatctgctg ttgatgaaag gtaacattgg
     1381 caagcctggt gcgggtatct gcccactacg aggtcactct aatgtacagg gcgaccgaac
     1441 cgtcggtatc accgagaaac cgtctgcaga gtttctggct cgcctgggtg agcgctatgg
     1501 cttcacccca cctcatgcac ctggacatgc tgcaattgcc agcatgcaag caatatgcac
     1561 ggggcaggct cgagcattga tctgcatggg gggcaacttt gcactggcaa tgccagatcg
     1621 ggaagcgagc gctgtaccgt taacgcaatt agatttggcg gtacacgtag ccactaagct
     1681 taaccgctct catctgctga ccgcacggca tagctatatt ctgccggttc tggggcgtag
     1741 cgagattgac atgcaaaaaa gcggtgcgca ggcggtaacc gttgaggatt caatgtcgat
     1801 gattcatgcc tcgcgtggcg tgttaaaacc cgccggtgta atgctgaaat cagagtgtgc
     1861 agtggtcgcg ggaatcgcgc aggcagcact accccacagc gtggtagcct gggagtatct
     1921 ggtggaagat tatgatcgca ttcgcaatga cattgaagct gtgttaccag agttcgccga
     1981 ctataaccag cgcatccgac atcctggagg ttttcacctg ataaatgcag ctgctgaaag
     2041 gcgctggatg acgccgtcag gtaaggctaa tttcattacc agcaaagggc tgttagaaga
     2101 tccctcttca gcgtttaaca gtaagctggt catggcgaca gtacgtagcc acgatcagta
     2161 caacacgacg atttatggta tggatgatcg ctatcgaggg gtattcggtc aacgagatgt
     2221 ggtctttatg agtgctaaac aagctaaaat ttgccgtgta aaaaacggcg aaagagttaa
     2281 tcttattgcg cttacgccag acggtaagcg cagctcacgc cgcatggata gattaaaagt
     2341 ggtcatttac cctatggctg accgctcact ggtgacctat tttccagaat cgaatcacat
     2401 gctaactctt gataaccacg atccattaag tggcattcct ggttataaaa gtattccggt
     2461 tgaattagaa ccatcaaatt aattcgcctc ctcatttctt ctgctgtcat ccgcacagca
     2521 gaagaatttc tcattgacta ttatcgcgca atttgctcac attgattaaa ttaaaccaca
     2581 cactataaga tataaacctc tgcctacagc tgtaagaaac tccgctcagt actgaagtgc
     2641 cagtcctatt tcctcttttc tccagcctgt tatattaagc atactgatta acgattttta
     2701 acgttatccg ctaaataaac atatttgaaa tgtatgcgac cacagtgaaa aacaaaatca
     2761 cgcaaagaga caactataaa gaaatcatgt ctgtaattgt ggttgtctta ttactgaccc
     2821 ttacgttgat agccattttt tcggcaattg atcagctggg tatttcagaa atgggtcgca
     2881 ttgcaagaga tcttacacat ttcattatca atagtttgca agactgaaaa taaacagcaa
     2941 attataaata tgaaatgtta aaaaagtatc gataaaaact ttattgtttt aaggagataa
     3001 aatgtcgctc gtttgtt
//
LOCUS       DAZOYL010000360         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZOYL010000360 DAZOYL010000000
VERSION     DAZOYL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.362"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUOY_003825"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUOY_003825"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR0684463.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUOY_003826"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUOY_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR0684464.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHUOY_003827"
     CDS             2805..>2992
                     /locus_tag="ACHUOY_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR0684465.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZOYL010000361         2977 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZOYL010000361 DAZOYL010000000
VERSION     DAZOYL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.363"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..865)
                     /locus_tag="ACHUOY_003828"
     CDS             complement(<1..865)
                     /locus_tag="ACHUOY_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0684466.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTN"
     gene            complement(1226..2230)
                     /locus_tag="ACHUOY_003829"
                     /pseudo
     CDS             complement(1226..2230)
                     /locus_tag="ACHUOY_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            2215..2592
                     /locus_tag="ACHUOY_003830"
                     /pseudo
     CDS             2215..2592
                     /locus_tag="ACHUOY_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2631..>2977)
                     /locus_tag="ACHUOY_003831"
     CDS             complement(2631..>2977)
                     /locus_tag="ACHUOY_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0684467.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT          735 a          670 c          736 g          836 t
ORIGIN      
        1 tattggtact actgagaaaa ccaactgtac tggtaacacc atggaaaacc catttttgtt
       61 gttctttatc atatagataa gaaccagaac cactatcgcc aaaaattggt gagtttttaa
      121 aaggggttct gttggtataa atgtaactct tgtttttcca gtccagatta aaaagacttg
      181 cgcttaacaa gtcgggatta tagttttgtc caaacagata ttgtttgccg tttaatatcg
      241 ttgaggttgt tccggcacct gctctgaagc caattatttg ttccttaccg ttgtagttta
      301 cgccatatct tttcatcgca tcttcgggag aaagtgagaa gtcggcactc tccgtaaccc
      361 ctgttgtttc cacgacgaat ttattcagac gatgaaccga aaagtcgcca gatgatactc
      421 tgttacttgc tttatatgct gtctgatccc atgactgtgt tgttattgca tgatgctgtg
      481 taccgttatg agtcgcagta acactgtacg cacctccaat tgatgttgtt gcacctttgt
      541 tggaaacagc agaaaaatca ggtacaggta gttctggaaa atgaaatatc tttccatctt
      601 tcatcattaa ttgaacattt gttgcacccg gtctgaactc tcctctgttt tgagcaaggt
      661 caagataatc gcgggtccat atattattta tatccagcat tgctgcaaat gctgtttcgc
      721 cataacttcc atatattgag gataaaataa taccgaggat aactttcttt cttgatgtac
      781 cgacactaac tttccgggtc agttcagaaa cagcaactaa tccacctgta atatgactat
      841 atttcagtga ataaatttta ttcatacgtg aactccgata tttctgagtt gtggttatgg
      901 gataacattt aattacaacg gggaactatc agtcatggta cgtaactgat aagggtataa
      961 tgataatcac tgtcagttat atttgtatga cagatgtaac aaaatgaaaa aaaagataag
     1021 cacaagtgat tgattggtca gatattgatc acttggtgtt tgtattttga actttgcgat
     1081 caaaatttga tctttgttgt ttccggtcaa tcctttacag gttttctgta ttatccgggg
     1141 gagttaagtc tgtcgatgtc cgacagactt ttgatgatgg aaacaacatg cagcgataat
     1201 gtgcatgctg ttttgcaggt ggaactcaga aggtgaactg gaactgtgcg accgcaccgt
     1261 aagtgttgtc cgtgccgatc aggcctgcaa catcaaggtg aaccacatca aacggtgaga
     1321 tccccacccc ggccgtgaat gcactgccgt cgttaccggc cagattctga cggtaaccgg
     1381 cgcgcagctg tgcccatttc caggcattaa attctgcgcc aatggcggca aactgacggt
     1441 tgctgtcgga ggtgaaaccg ctggccgggg tcagatccac atcaaatgcg gtggtgaaca
     1501 tcgcgttgtg ccaggagaca ccggccgtca cctgcgggcg gatcctgaag gtttccgtga
     1561 taccgttcac ttcttttgtc tcaatactac gggggatcag gttctgtgcg accagtccca
     1621 gcgtccagtt gtcatccagg tccgtactgg caccgatgtc ggcgttgatg ccatttttcg
     1681 tgttgtggta gcggtcgcct ttgaaggcgc tgctgtcata atttctgacc agcacgttgt
     1741 agttgaacag gtcgacgcgc tggaatttgg gggggaagcc cagagaccat ttctgcccgg
     1801 ccgtttccag ttccgtagcc agagcaatac ccacatcagt gatcagtgcc gcgcgggcaa
     1861 aggcctgtga tgtcaggcgg cttttgtcca cctcctgccc ggtgctgttt gccacctctt
     1921 ccaggtaagt gaggtcagca tcagagactt taccgtcgac actgacggtg ccgtgtgatt
     1981 taaccatcag tgcggcagag aggcgatcac cgggaagggc cgccacagca gaaactccca
     2041 gctgtgcggc ggcatgtgtg ttacggaagt cccggaggtg ttctttcagg cgtgcggcag
     2101 cctcaggaac accgtggtta ctttcgatgg cgcggtcgaa ggccttccac tcatcactga
     2161 tccggtcggc gttgtcggtg atattatccg gattggagag ctgcgcgccg acggacggaa
     2221 gcaggatgct gggctcatgc gcgacgcaaa atccacgatg tatatatcag tactaaaagc
     2281 gcgacggcgg aagaagctct gaaactaatc ggtgaactgt acgccataga gcacgatata
     2341 cgcgggttac cggtgtctga acgcctggcg gtcaggcaaa tgcagagtag accgctcctg
     2401 acttccctgt ataagctgat gcaggagaaa gaacacacgt tatcgaagaa atgtcgtctg
     2461 ggagatgcgt tccggtatat caggaagcac tgggctgcgt tgtgcaactt ctgtgatgat
     2521 ggcctggctg aggtagataa taatgcagca gaaagagcgc ttcgtgcagt ctgtctcggg
     2581 aagaaaactt tttcgaggat cactcattcc gtgtctgact gcttcagggt ttaattttgc
     2641 ttctgctgac tgaatgatga ttgggcagca agtgcaatat cccacataaa tccagttaac
     2701 tcacgagcca ccgccgtaat ggctacttgt gattttttcc cttttgctgt cagagttcgg
     2761 taacgagaac acaatcgttg ttgagctttc catgcaatat ctttggaata ttgtgttaca
     2821 gagtccggtc ggtaatatat aagccatgct ccaacttttg caggtgtacg ataagaccaa
     2881 gcggcttcgt aaagcagacg tctcagttcg ctgtttccaa cttttgttat tcctctggga
     2941 cgaatactgt ttccactgga atattcacct ggcacca
//
LOCUS       DAZOYL010000362         2951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZOYL010000362 DAZOYL010000000
VERSION     DAZOYL010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.364"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(89..757)
                     /gene="artM"
                     /locus_tag="ACHUOY_003832"
     CDS             complement(89..757)
                     /gene="artM"
                     /locus_tag="ACHUOY_003832"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR0684468.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(757..1473)
                     /gene="artQ"
                     /locus_tag="ACHUOY_003833"
     CDS             complement(757..1473)
                     /gene="artQ"
                     /locus_tag="ACHUOY_003833"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR0684469.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1480..2211)
                     /gene="artI"
                     /locus_tag="ACHUOY_003834"
     CDS             complement(1480..2211)
                     /gene="artI"
                     /locus_tag="ACHUOY_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR0684470.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2229..>2951)
                     /gene="artP"
                     /locus_tag="ACHUOY_003835"
     CDS             complement(2229..>2951)
                     /gene="artP"
                     /locus_tag="ACHUOY_003835"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR0684471.1"
                     /translation="IQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSLLR
                     VLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQNLI
                     EAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEPQV
                     LLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGHIV
                     EQGDASCFTEPQTEAFKNYLSH"
BASE COUNT          724 a          812 c          746 g          669 t
ORIGIN      
        1 ccggcacagg caccgtgctg atgtctgatg cgacgctggc gcgtcttatc agacctacaa
       61 acccccccgg cgaatgtacg cagctacatt aattccgccg ttcgaatgcc agtgctttgc
      121 gctcgatcag acgcatcatc agcgtcagca ggccgttgac gaccaggtaa ataatccctg
      181 ccgcaccgaa caccattaca tcgtaggtgc gtccgtacaa caactggctg tatcccatca
      241 cttccatcag cgtaatggtg taagccagag aggtactttt gaacaccagc accacttcgt
      301 tggaataaga agagagcgag cgtttaaagg catacggcag caggagcgcc agcgtatctt
      361 ttttgctcat tcccagggcg ctacaggact gccactgacc ttctgggatc gcacgaattg
      421 caccgtaaaa cagctgcgtg gtatacgccg cactgttcag cgataacgca atcagcgcac
      481 ataaccacgg ttctgacaac aaatgccaca gtgccggata ctcctgcaac gtcggaaact
      541 ggcccggccc gtaataaatc aggaagatct gcaccagcag cggcgtaccg gtaaacagcg
      601 tgatataacc ccgcaccagc cacaccagca ccggcgtttt cagcgtcagg atgatggtaa
      661 aaatcaatgc cagaatcagt gccacaatca gcgaggcaac ggttagcgtc aggctggtgt
      721 gtagcccttt catcagttcg ggtaaatact caaacattag ctgggcctcc gctcaaaacg
      781 tgtcgcgcgc aggtcaatgc gtttgagaat gtactgactg agcagggtga tcaccaggta
      841 aatcgccgcc gccacaatgt accaggtaaa tggttcctgg gtacgagtag cgatgctttt
      901 tgtttgcagc attaaatcat tcacactaat caaactgacc agcgcggtat ctttcagcag
      961 caccagccac tggttaccga ggccaggcag cgcatgacgc cacatctgcg gcatcaccag
     1021 acggaaaaag atagccgatt tcgacagccc cagcgcctga ccagactccc actgacccac
     1081 cggcaccgct ttcaacgcgc cccgaagcgt ttgcgaggca taggcggcat acagcagtga
     1141 cagagcgatg acaccacaaa ggaacgggct aacgtcgaag ttctcaatgt ccatctgcac
     1201 tgggatctgc acgaacccaa gattgatagt gaagccatcc gaaagcgtca gcagcagctg
     1261 cgaggagcca aaatagataa acagcaccac cagaatttct ggcaggccac gcagaatggt
     1321 taccagcgct gaacctgccc acgcgacagg acgccatttt gccgactccc ataccgcaaa
     1381 gaacatcgcc agcgccagcc cgacaatcaa tgcacaaacg gcaaggccga cggtcatccc
     1441 ggcggcgctt gctaaaggaa aaaattcatt catcaggaat tacttctgga accatttgtt
     1501 gtagatggtt tcgtaagtgc catctttctt cactttttcc agcgcagtgt tgagtttctg
     1561 ctgcagctca gtgttgccct gacgtaccgc gatgccgagg ccagtgccga agtaatcttt
     1621 atcggtcact ttgtcgccca ccgccgccag tttcgggtta tctttcagcc actcagtgac
     1681 cactgcggtg tcaccgaaga cgccgtcgat acgcccgttt tgcagatcca gttttgcgtt
     1741 ctggtagctg tcatacggaa cggtagtgat ttccgggtgc ttatccataa tgaatttctg
     1801 gtgtgtcgtc ccgttctgta cgccgacttt tttgcctttc agctgatcaa cactggtgta
     1861 tttgccttgc tgacccacaa acagggcaga gttgtcatag tacggggtgg taaacagcac
     1921 ctgcttttca cgctccggag tgatatccat gcccgccatc acggcttcta cgcgacggaa
     1981 tttcaggctt gggatcaggc tgtcaaacgc ctggttagag aaagtgcagg ttgcatcaat
     2041 ctctttacac agcgcttgtg ccaggtcgac gtcaaaacca acgatctggt tgtttgcatc
     2101 aatcgattca aacggaggat aggatgcttc ggtagcaaaa cgaatggttt cggcagctgt
     2161 ggcggaaaga ctaaaacctg caattaacgc ggcaatcaga acttttttca ttgttgttat
     2221 cccgaatctt agtgagagag atagttttta aatgcttcgg tttgcggctc ggtaaagcag
     2281 ctcgcgtcgc cttgttctac gatatgacca ttttccatat acaccactcg gctggcggtt
     2341 ttacgcgcca cttcaacttc gtgggtgacg atcacctggg taatattcgt ttctgccagc
     2401 tcacgaatga tgctgacgat ttgtgccgta atttccgggt ccagtgcggc ggtcggttca
     2461 tcgaacagca gtacctgcgg ttccatcatc aacgcacggg caatagcaac acgctgctgc
     2521 tgaccaccag aaagatgcag cgggtaacga tcgctataag gtttgagacg cagacgttcc
     2581 agcagttttt ctgcacgggc cagcgcctga tctttactca accccagtac acggcagggc
     2641 gcttcaatca ggttttgctg cacggtcaga tgcggccaca ggttgtattg ctgaaacacc
     2701 atgccaacgt tacgacgcaa atcgcgaatc gctttgtcag agggtgtttt ggtgaaatcg
     2761 aaatggttgc ctgcaatgtt gagcgtaccg gagcgtggca tctcaagcag attgagtaca
     2821 cgcagcagcg agcttttacc cgcgccgctg gggccaagta acaccagcgt ttcgccctgt
     2881 gggcaatcca gcgtgatatc gaacagcgcc tgatgcgcgc cgtagaagca attaatgccg
     2941 tttaattgaa t
//
LOCUS       DAZOYL010000363         2939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZOYL010000363 DAZOYL010000000
VERSION     DAZOYL010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.365"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHUOY_003836"
     CDS             37..264
                     /locus_tag="ACHUOY_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0684472.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHUOY_003837"
     CDS             complement(265..639)
                     /locus_tag="ACHUOY_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR0684473.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUOY_003838"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUOY_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHUOY_003839"
     CDS             complement(2099..2674)
                     /locus_tag="ACHUOY_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0684474.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          696 c          688 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctt atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt gctgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt agatttgttg tctatagcta
     2761 tagtgactct tctatgtaga cattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DAZOYL010000364         2922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZOYL010000364 DAZOYL010000000
VERSION     DAZOYL010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.366"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(112..188)
                     /locus_tag="ACHUOY_003840"
     tRNA            complement(112..188)
                     /locus_tag="ACHUOY_003840"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(152..154),aa:Asp,seq:gtc)
     gene            complement(321..1052)
                     /gene="dnaQ"
                     /locus_tag="ACHUOY_003841"
     CDS             complement(321..1052)
                     /gene="dnaQ"
                     /locus_tag="ACHUOY_003841"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR0684475.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1117..1584
                     /gene="rnhA"
                     /locus_tag="ACHUOY_003842"
     CDS             1117..1584
                     /gene="rnhA"
                     /locus_tag="ACHUOY_003842"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0684476.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1581..2303)
                     /locus_tag="ACHUOY_003843"
     CDS             complement(1581..2303)
                     /locus_tag="ACHUOY_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0684477.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2337..>2922
                     /gene="gloB"
                     /locus_tag="ACHUOY_003844"
     CDS             2337..>2922
                     /gene="gloB"
                     /locus_tag="ACHUOY_003844"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR0684478.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKEL"
BASE COUNT          783 a          715 c          686 g          738 t
ORIGIN      
        1 catttacagc ataacaaaac catagaccga agaaatagcg ggatttaaat gcaaaaaaac
       61 cgcaaaatta aaaatcttgc ggttctctga actcattttc atgagtgaat ttggcggaac
      121 ggacgggact cgaacccgcg cccccctgcg tgacaggcag gtattctaac cgactgaact
      181 accgctccgc gttgtgttcc gttgggaacg aggcgaatat tacggattgc ctcacccttc
      241 gtcaacggtt tttctcatct tttgaatcgt ttgctgcaaa aatcgcccaa gccgctattt
      301 ttagcgcctt ttacaggtat ttatgcccgc cagaggcagc ttccgccctt cttctccacc
      361 agatcaagac gggcttcatg agctgcaagc tcttcatctg tcgcaaaaac aacgcgtaac
      421 ttacttgcct gacgtacaat gcgctgaatt gttgcttcac cttgttgctg ctgtgtctct
      481 ccttccatcg caaaagccat cgacgtttga ccaccggtca tcgccagata aacttccgca
      541 aggatctgtg catcgagtaa tgccccgtgt agcgttcgtt tactgttatc tatttcgtag
      601 cgggcacata acgcatcgag gctgttgcgc ttaccaggaa acattttcct cgccaccgca
      661 aggctatcgg tgaccttaca gaaggtattg gtcttcggaa tatcgcgctt aagcaacgaa
      721 aactcgtagt ccataaagcc gatatcgaac gctgcgttat ggatcaccaa ctccgcgccg
      781 cggatatagt ccatgaactc atcggctact tcggcaaacg tgggcttatc gagcaaaaat
      841 tcatcggcaa taccatgtac gccaaaggct tccggatcca ccagccgatc gggtttgaga
      901 taaacatgga agttattgcc cgtcaggcga cggttcacca cttcaacggc accaatctca
      961 atgatcttgt ggccttcata gtgcgcacca atctggttca taccggtggt ttcggtatcg
     1021 agaacgatct ggcgtgtaat tgcagtgctc atagcggtca tttatgtcag acttatcgtt
     1081 ttacagttcg attcaattac aggaagtcta ccagagatgc ttaaacaggt agaaattttc
     1141 accgatggtt cgtgtctggg caatccagga cctgggggtt acggcgctat tttacgctat
     1201 cgcggacgcg agaaaacctt tagcgcaggc tacacccgca ccaccaacaa ccgtatggag
     1261 ttgatggccg ctattgtcgc gctggaggcg ttaaaagaac attgcgaagt cattttgagt
     1321 accgacagcc agtatgtccg ccagggtatc acccagtgga tccataactg gaaaaaacgt
     1381 ggctggaaaa ccgcagacaa aaaaccagta aaaaatgtcg atctctggca acgtcttgat
     1441 gctgccctag ggcaacatca aatcaaatgg gaatgggtta aaggccatgc cggacacccg
     1501 gaaaacgaac gctgtgatga actggctcgt gctgcggcaa tgaatcccac actggaagat
     1561 acgggctacc aagtcgaagt ttaagcttgt ggtttacgac attgccgggt ggctccaacc
     1621 gcctggcgaa ttcgtggctt gtttttactc tgtttcatcg ggtttagcgt taaaggaata
     1681 gtccgtttcc gggcaacaat aagttgtaag caaccaagcg caggaatatg cgcattcaat
     1741 agttttcctc cgtgtttgtt ccacgggaga acgtggaaac ggctggcgtg cagcacttca
     1801 aaattcaaca aagagagcca gtccagttgc cgcatcagag taaacatccg gctgttatag
     1861 ggcgaggttt ttcgcaatac cggcacaagt ttgcgtaatc ccattaaact aatgggattg
     1921 aagccactaa tcaccagcca gccatcatca atcaataccc gatcggtttc acgcaataaa
     1981 cgatgcggat cggtgcacca cggcaatgta tgtgccagta gacaaacatc aacggattta
     2041 tcggcaaaag gaagatgaag tggatccgcc tgcacctgga cgggcattcc ttgcgcagaa
     2101 acattcactt gatgagaaac cgcgcacgct tcgcaattga tttctgcgct taaattgcca
     2161 atcttaagca gatgaaaacc atacatttta gtgaaccacg ggttgagctg gcgctccagc
     2221 gccttgcgat aaagctctcc ccagggcaaa tcgccccagc gatcaggagc cacgacagtt
     2281 tgagggactc ttgccggttt catcacaacc ttccgtttca cactaagagg taatctatga
     2341 atcttaacag tattcccgcc tttgatgaca attacatctg ggttttgaat gatgaagcag
     2401 gtcgctgcct gattgtcgat cccggagacg cagagccagt attaaacgcc attgccgcca
     2461 ataactggca accggaggcc atatttctca cccaccatca tcacgatcac gttggcggcg
     2521 taaaagaact ggtggaaaag tttccgcaaa ttgtggtgta tggtccacaa gagacacaag
     2581 ataagggaac aacacaggta gtcaaagatg gcgaaactgc cttcgttttg gggcatgaat
     2641 ttagtgtaat tgccacaccg ggtcacactt taggacatat ctgttacttc agtaaacctt
     2701 atctattttg cggcgacacg ctgttttctg gtgggtgtgg taggttgttt gaagggacag
     2761 catcacaaat gtatcaatca cttaataagt taagtgcgtt acctgacgat acattggtat
     2821 gttgtgctca tgaatatacg ttatcaaata tgaagtttgc tttgagtatt cttccgcacg
     2881 atttgtccat aaatgattat tatcgtaaag ttaaggagtt ac
//
LOCUS       DAZOYL010000365         2893 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZOYL010000365 DAZOYL010000000
VERSION     DAZOYL010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.367"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHUOY_003845"
     CDS             <1..493
                     /locus_tag="ACHUOY_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0684479.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHUOY_003846"
     CDS             493..1083
                     /locus_tag="ACHUOY_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR0684480.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1832..2110
                     /locus_tag="ACHUOY_003847"
                     /pseudo
     CDS             1832..2110
                     /locus_tag="ACHUOY_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2170..2658
                     /locus_tag="ACHUOY_003848"
     CDS             2170..2658
                     /locus_tag="ACHUOY_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0684481.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2682..>2893
                     /locus_tag="ACHUOY_003849"
     CDS             2682..>2893
                     /locus_tag="ACHUOY_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0684482.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEY"
BASE COUNT          752 a          632 c          825 g          684 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagagcacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctg
//
LOCUS       DAZOYL010000366         2891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZOYL010000366 DAZOYL010000000
VERSION     DAZOYL010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.368"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            98..2476
                     /gene="ppsA"
                     /locus_tag="ACHUOY_003850"
     CDS             98..2476
                     /gene="ppsA"
                     /locus_tag="ACHUOY_003850"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR0684483.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2533..>2891)
                     /locus_tag="ACHUOY_003851"
     CDS             complement(2533..>2891)
                     /locus_tag="ACHUOY_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR0684484.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
BASE COUNT          678 a          726 c          795 g          692 t
ORIGIN      
        1 aacgattcac cgtttttttc atccggttaa atatgcaaag ataaatgcgc agaaatgtgt
       61 ttctcaaacc gttcatttat cacaaaagga ttgttcgatg tccaacaatg gctcgtcacc
      121 gctggtgctt tggtataacc aactcggcat gaatgatgta gacagggttg ggggcaaaaa
      181 tgcctccctg ggtgaaatga ttactaacct ttccggaatg ggtgtttccg ttccgaatgg
      241 tttcgccaca accgccgacg cgtttaacca gtttctggac caaagcggcg taaaccagcg
      301 catttatgaa ctgctggata aaacggatat tgacgatgtt actcagcttg cgaaagcggg
      361 cgcgcaaatc cgccagtgga ttatcgacac tcccttccag cctgagctgg aaaacgccat
      421 ccgcgaagcc tatgcacagc tttccgccga tgacgaaaac gcctcttttg cggtgcgctc
      481 ctccgccacc gcagaagata tgccggacgc ttcttttgcc ggtcagcagg aaaccttcct
      541 caacgttcag ggttttgacg ccgttctcgt ggcagtgaag catgtatttg cttctctgtt
      601 taacgatcgc gccatctctt atcgtgtgca ccagggttac gatcaccgtg gcgtggcgct
      661 ctctgccggt gttcagcgga tggtgcgctc cgacctcgca tcttctggcg tgatgttctc
      721 cattgatacc gaatccggct ttgaccaggt ggtgtttatc acttccgcat ggggccttgg
      781 ggaaatggtc gtgcagggtg cggttaaccc ggatgagttt tacgtccata aaccgacact
      841 ggcggcgaat cgcccggcta tcgtgcgtcg caccatgggg tcgaaaaaaa tccgcatggt
      901 ttacgcgccg acccaggagc acggcaagca ggttaaaatc gaagacgtac cgcaggaaca
      961 gcgtgacatc ttctcgctga ccaacgaaga agtgcaggaa ctggcaaaac aggccgtaca
     1021 aattgagaaa cactacggtc gcccgatgga tattgagtgg gcgaaagatg gccacactgg
     1081 caaactgttc attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc aggtcatgga
     1141 gcgttatacg ctgcattcac agggtaagat tatcgccgaa ggccgtgcta tcggtcatcg
     1201 catcggcgcg ggtccggtga aagtcatcca tgagatcagc gaaatgaacc gcatcgaacc
     1261 tggtgacgtg ctggtcactg acatgaccga cccggactgg gaaccgatca tgaagaaagc
     1321 atctgccatc gtcaccaacc gtggcggtcg tacctgtcac gcggcgatca tcgctcgtga
     1381 actgggcatt ccggcggtag tgggctgtgg tgatgcaaca gaacggatga aagacggtga
     1441 gaacgtcact gtttcttgtg ccgaaggtga taccggttac gtctatgcgg agttgctgga
     1501 atttagcgtg aaaagctcca gcgtagaaat gatgccggat ctgccgttga aagtgatgat
     1561 gaacgtcggt aacccggacc gtgctttcga cttcgcctgc ctgccgaacg aaggcgtggg
     1621 ccttgcgcgt ctggaattta tcatcaaccg tatgattggc gtccacccac gcgcactgct
     1681 tgagtttgac gatcaggaac cgcagttgca aaatgaaatc cgcgagatga tgaaaggttt
     1741 tgattctccg cgtgaatttt acgttggtcg tctgactgaa gggatcgcga cgctgggtgc
     1801 cgcgttttat ccgaagcgcg tcattgtccg tctctctgat tttaaatcga acgaatatgc
     1861 caacctggtc ggtggtgagc gttacgagcc agatgaagag aacccgatgc tcggcttccg
     1921 tggcgcggga cgctatgttt ccgacagctt ccgcgactgc ttcgcgctgg agtgcgaagc
     1981 agtgaaacgt gtgcgcaacg acatggggct gaccaacgtt gagatcatga tcccgttcgt
     2041 gcgtaccgta gatcaggcga aagcggtggt tgaggaactg gcgcgtcagg ggctgaagcg
     2101 tggtgagaac gggctgaaaa tcatcatgat gtgtgaaatc ccgtccaacg ccttgctggc
     2161 cgagcagttc ctcgaatatt tcgacggctt ctcaattggc tcaaacgata tgacgcagct
     2221 ggcgctcggt ctggaccgtg actccggcgt ggtgtctgaa ttgttcgatg agcgcaacga
     2281 tgcggtgaaa gcactgctgt cgatggctat ccgtgccgcg aagaaacagg gcaaatatgt
     2341 cgggatttgc ggtcagggtc cgtccgacca cgaagacttt gccgcatggt tgatggaaga
     2401 ggggatcgat agcctgtctc tgaacccgga caccgtggtg caaacctggt taagcttggc
     2461 tgaactgaag aaataaaata aatccccggc ggcgttcagt cgccggggtt atgtgatccc
     2521 cgaagatgaa acttattcaa tctcttcaca gacatcctga gttaaccgcc gcataatatc
     2581 ttttcttaac aaaaactttt gtattttacc tgaggcagta cgcggtagtt tttcgattac
     2641 cacgatatgt tcaggatatt tatattttgc gacccgttta cggctaaaaa aagccaccac
     2701 ctcttccagc gataatgaat gatgcggcgc tttcagcacg acataagcgc atgatcgttc
     2761 acctaaacgt tcatcgggca ttgcaaccac acaggcatcg tgaattttag gatgctgcaa
     2821 taaaatatct tctacttcac ggctgctaat attttcgccg ccgcggacaa taatatcttt
     2881 tttgcgtccg g
//
LOCUS       DAZOYL010000367         2877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZOYL010000367 DAZOYL010000000
VERSION     DAZOYL010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.369"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHUOY_003852"
     CDS             complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHUOY_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR0684485.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDG"
     gene            1134..1652
                     /gene="mddA"
                     /locus_tag="ACHUOY_003853"
     CDS             1134..1652
                     /gene="mddA"
                     /locus_tag="ACHUOY_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR0684486.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1649..2098
                     /locus_tag="ACHUOY_003854"
     CDS             1649..2098
                     /locus_tag="ACHUOY_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0684487.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHUOY_003855"
     CDS             complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHUOY_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR0684488.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHUOY_003856"
     CDS             complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHUOY_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR0684489.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2786..>2877
                     /gene="yncL"
                     /locus_tag="ACHUOY_003857"
     CDS             2786..>2877
                     /gene="yncL"
                     /locus_tag="ACHUOY_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR0684490.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          756 t
ORIGIN      
        1 aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc
       61 tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag
      121 cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt
      181 agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta
      241 agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata
      301 ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa
      361 acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc
      421 accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc
      481 gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata
      541 taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga
      601 tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag
      661 cccagcaccc agtcgctagg ctgataatca ggatgattcg actccacaac acggctcacc
      721 gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc
      781 atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct
      841 tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca
      901 tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgccc cataatgctc
      961 tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg gttttgtttg
     1021 cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg
     1081 ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca gacatgtcca
     1141 tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac cacgccgtgt
     1201 tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc attgcctggt
     1261 ttgaagcgcg gaccatagca ggttatccgg tgctggtaag tgaagaagat ggcgtagtga
     1321 cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat accgtggaac
     1381 attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc
     1441 gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc
     1501 aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt tgtcgtcacc gcacaaatgc
     1561 cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg
     1621 acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg cttttttgat
     1681 tgccgccgga attggtctgg tagtccagaa cacgctgatg gtgcgcatca cccagacctc
     1741 ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc
     1801 tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg
     1861 gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta tcagcggata
     1921 tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc taattggcgg
     1981 tctgatgctg gatatcttcc gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat
     2041 ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct cattttaatc
     2101 agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgct
     2161 tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc
     2221 gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc
     2281 tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc
     2341 cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc
     2401 aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa actcatcggc
     2461 catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga
     2521 aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga
     2581 tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt
     2641 ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc
     2701 ccttatccat cttcagaatg agtattattg cggattttct taacccgtac tatacacata
     2761 ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa
     2821 tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct ggtttat
//
LOCUS       DAZOYL010000368         2858 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZOYL010000368 DAZOYL010000000
VERSION     DAZOYL010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.370"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            47..679
                     /gene="comR"
                     /locus_tag="ACHUOY_003858"
     CDS             47..679
                     /gene="comR"
                     /locus_tag="ACHUOY_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR0684491.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(741..1280)
                     /locus_tag="ACHUOY_003859"
     CDS             complement(741..1280)
                     /locus_tag="ACHUOY_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0684492.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1507..2811)
                     /gene="ndh"
                     /locus_tag="ACHUOY_003860"
     CDS             complement(1507..2811)
                     /gene="ndh"
                     /locus_tag="ACHUOY_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR0684493.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          691 a          782 c          690 g          695 t
ORIGIN      
        1 tttttataat aacctttcat tcacagtcac caggttcaac ggcatcatgg caactgactc
       61 aacacaatgt gtaaaaaaaa gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct
      121 tgataaggcc atgaaattgt tctggcaaca cggttatgaa gcgacttctc ttgcggacct
      181 cgtcgaagcg accggagcca aagcgcccac gctatacgcg gaatttacca acaaagaggg
      241 gttatttcgt gccgttctcg accgctatat cgatcgtttt gccgctaagc atgaagcaca
      301 gctgttttgt gaagagaaaa gcgtggagtc tgcgctggct gactattttg ctgccatcgc
      361 caactgcttt accagcaaag acaccccggc aggctgcttc atgatcaaca actgcaccac
      421 cctctcccca gattcaggag atatcgccaa tacgttgaaa tcacgccatg cgatgcaaga
      481 gcgcactttg cagcagtttt tatgtcaacg acaagcgcgc ggggaaatcc cgacccactg
      541 tgacgtgaca catctggcag aattccttaa ttgtattatt caggggatgt cgatcagcgc
      601 acgcgaaggt gcatcgctgg aaaaactgat gcagattgcc ggaacgactt tgcgtttatg
      661 gcccgaactg gtgaaataag tcgcaaaaaa agcccctcag cctgagcgaa tgaggggcca
      721 aattgcagag tacagccttg ttatactttg ttattcaaaa tcagttgccc gttactatct
      781 agcgggatct gcgtacccgg atcgcggtcc atgcggattt tgccctgctg atcgccaatc
      841 ttataggtca catcataacc gagcattttt tctgacttgt catacaccgt tttacaacgc
      901 tgttgcgtag tcgtgtaagt atcgctttcc tggagagagc cctggatctg gttaccggca
      961 tatccaccac ccagcgcccc cacaacagtg gcgacatctt taccacgacc accaccaaac
     1021 tgatgcccta tcacgccgcc agcaacagcg ccgagcaccg acccggtaat gcgattttca
     1081 tcctgcaccg gtcgacgatg ggtcactgtg acgttgcgac actcctgacg cggtgtttta
     1141 accgtttcct tgattggggt tgcagaaaca acctgagcgt attgcgggcc ccgttcaaac
     1201 acgttcagac tggccactgc cgctacgccc agcgcagctg cgacaccaat cccgataccc
     1261 gccaacattg atttattcac gggaacacct ccttctttgc agttatgcca atcatgcaag
     1321 cgaaggagaa aagcagctat cagacagaag atgaaaagcg cgggaatggc tggaaatact
     1381 taaggattcg gagaagtctg aaggaatgcc tgatgcgacg cttaacgcgt cttatcaggc
     1441 ctaccgctcg taccatttgt ccagggcgga tgaggcgttt atgccacatc cgccagtgta
     1501 cgtcgattaa tgcaacttca aacgcggacg gataacgcgg ttaatactcc ccaccagcat
     1561 cattaatccg gttttaaagt aaccatgcag cgcaatctga tgcattcggt atagcgagat
     1621 atatacaaag cgcgcaattc gtccttcaat catcattgag ccgcgcgtca ggttacccat
     1681 caggctaccg acagtggaga agttcgacag cgataccagc gaaccatgat ctttatactg
     1741 ataatttttc agcggtttac cgttcatctg cgccagaata tttttcattg cgcaggtcgc
     1801 catctggtgt gcagcctgag cacgcggcgg aacaaagccc ccttccggac gcgggcatga
     1861 cgcgcagtcg ccaatagcgt aaatgtctgg atcgcgggtg gtttgcagcg tcggttccac
     1921 caccagctgg ttgatacggt tggtttcaag accaccgata tctttcagga agtctggcgc
     1981 tttgatcccg gctgcccaca ccatcagatc agcctcaata tattcaccat ctttagtgtg
     2041 caggccgcct tcatcagcac tggtgaccat ggtttgcgtc agcacgcgaa cgccaagttt
     2101 cgttagctcg ttgtgggccg cagcagagat acgtggcggt aatgcaggca aaatacgttc
     2161 tcccgcttct accagcgtta cgttcagggc ttcgttggtc aggcctttgt aaccgtagct
     2221 gtgcagttgc ttgaccgcat tgtacaattc agcggagagt tctacacccg tcgcgccgcc
     2281 gccgacaatc gcaatgttca ctttaccatt cgcgcccagg ttggcggagt atttcaggaa
     2341 cagattcagc atctcctggt ggaagcgacg cgcctggtgc gggttatcga ggaaaatgca
     2401 gttctctttg acacctggcg tattgaaatc gttagaggtg ctacccagcg ccattaccag
     2461 ggtgtcatag gcgattttac gttccggaac cagcagttca cctttctcat cgcgcagttc
     2521 tgcaatagtg attgttttcg cttcacgatc aatatcaatg acggaaccca gctggaactg
     2581 gaaaccatga ttgcgcgcat gggccagata gctcaacgca tcgacgcctt catcaagcga
     2641 gccagtcgcc acttcgtgca gcagcggttt ccacagatgg ctgtggttac gatcgaccag
     2701 cgtaattttg gcttttttct tgcgtcccag cttatgcccc agctgtgttg ccatttccag
     2761 cccaccagca ccgccgccga caatcacaat ttttttcaat ggcgtagtca acgtgacccc
     2821 cttaaattta ttaaccaatt gttaattaat ggttataa
//
LOCUS       DAZOYL010000369         2852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZOYL010000369 DAZOYL010000000
VERSION     DAZOYL010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.371"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..1028
                     /locus_tag="ACHUOY_003861"
                     /pseudo
     CDS             1..1028
                     /locus_tag="ACHUOY_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1044..1792
                     /locus_tag="ACHUOY_003862"
                     /pseudo
     CDS             1044..1792
                     /locus_tag="ACHUOY_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            1794..>2852
                     /locus_tag="ACHUOY_003863"
     CDS             1794..>2852
                     /locus_tag="ACHUOY_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684494.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          896 a          572 c          590 g          794 t
ORIGIN      
        1 agttatattg tcacgtttaa ccagtatctt gaatcattaa ggttaaatac atacgtaagt
       61 ttggctcgta atacttactg ggacgccagc agtaatgtga attattcatt atcacttagc
      121 cgcgattttg atatcgggcc attaaaaaac gtctccgctt ctctaacatt tagccgaata
      181 aactgggaag aagacaacca ggatcaactg tacctaaata tttcgattcc ctggggaacc
      241 agtagaacat tgagctatgg tatgcaacga aatcaggata acaagatttc gcatactgct
      301 tcgtggtatg actcttccga tcgaaataat tcctggagcg tttctgcttc aggcgacaat
      361 gatgaattca aagatatgaa agcgtcacta cgcgccagtt atcagcataa taccgagaac
      421 ggtcgtctct atctctccgg gacatcacag cgagacagtt attattctct gaatgccagt
      481 tggaatggtt cattcacggc gactcgtcac ggagccgctt tccatgacta tagtggtagt
      541 gctgactcgc gttttatgat cgacgcagat ggtgcagaag atattccgtt gaacaataaa
      601 cgcgcggtaa ctaatcggta tggcatcgga gttattccat cagtcagcag ttacataaca
      661 acatcattaa gcgttgatac ccgaaatctt ccagaaaatg tggatatcga aaactcggtt
      721 atcaccacca ccttaaccga aggtgctatt ggctacgcca aacttgatac cccaagggct
      781 atcaaatcat gggggttatt cgcctggcag atggtagtca cccaccactg gggattagcg
      841 taaaagataa aaccagccat aaagaattag gactggttgc tgatggcggc tttgtatacc
      901 tcaacggcat tcaggatgac agcaaactta ctttacgctg gggtgataaa tcctgtttta
      961 ttcaaccacc caatagcagc aacttaacca ccggaacggt tattttaccg tgtattagcc
     1021 aaaattaatt caggatgaaa ctaatgcgct acttaaacac aaagaatctc attgcagcag
     1081 gagtattact cgcctgcatg agcagcattg cctggggtgc catcatcccg gaccgtaccc
     1141 gtatcataat gaatgagtca gataaagggg aagcattaaa acttactaat caaagcaaaa
     1201 atctacccta tctggcgcaa acatggatag aagatacgaa aggcaataaa tcacgtgact
     1261 ttattgtcac ggttccccct atggtacgtt taaatcccag tgagcaaatt caaataagaa
     1321 tgataagtca ggaaaaaatt gctcaactgc ctaatgacag agaaacgctc ttctatttta
     1381 acgtgcgaga aataccgcca aaaacagata aaaaaatgtg atgcaagtca ctatgcagca
     1441 cgcattgaaa ctattctggc gaccaaaagc tattgaatta gaagatgatg gggtaatgtc
     1501 ttacgaaaaa gttgaaatca tccgtagaaa tgatggttcg atccgcttta acaataagat
     1561 gccttatcac gttacgctgg gttatatcgg aacaaacgga atcacaatgt tgccacaaac
     1621 gcaaagttta atggtaaccc cttatagcca tgcagatacg cagtttaaaa atgttccgtc
     1681 agcttttcag gttggttata tcaatgattt tggtggatta agtttttatg aaataaactg
     1741 cccgacggtt aataactctt gtaatgtttc cgtagccaaa cgagataaat aatatgcgct
     1801 acttattaat tgtaattata tttattatag gttttagtgc attacctgta tgggcaatgg
     1861 actgctatgc tgaacatgaa gggggaaata ccgtagtcat tggttacgta ccaagaattt
     1921 ctatccccag taatggtaaa aaaggtgata agatctggca aagcagtgag tattttatga
     1981 atgtcttctg taataatgca ttacctgccc catcacctgg agaagaatac ccatcagcct
     2041 ggactaatat aatgatgttt ttaccaggcg gtcaggactt ttataatcag aactcctata
     2101 tttttggcgt aacatacaat ggagttgatt atgattcgac tgcccctaat gctatcgcag
     2161 caccagaatg cattgatata aaaggagcgg gtacctttaa taatcactat aaaaaccccg
     2221 ctgtctgtag tggaggtcct gagcctcaat tatcagtaac ctttccagct cgcgtacaac
     2281 tctatattaa attagcaaaa aatgccaata gagttaataa aaacctggtg ttacctgatg
     2341 aatatatcgc tctagagttt aaaggtatga gtggcgcagg agctatagaa gtagacaaaa
     2401 atttgacctt caggattcgt ggattaaata atattcatgt ccttgactgc tttgttaatg
     2461 tcgcactgga accagctgat ggcgttgttg actttggtaa aataaattcc cgaacaatta
     2521 aaaataccag cgttagtgag acgtttagtg tagtcatgac aaaagatcct ggtgcggcct
     2581 gtactgagca attcaatatt ttagggagct ttttcactac ggatattttg agtgattata
     2641 gccatctgga tatgggtaat ggactgctat tgaagatatt tcataaagat ggaacagcaa
     2701 cggaatttaa ccgcttctca caatttgctt ctttttcatc gtctagtgca ccttcggtca
     2761 ccgcaccatt cagggcagaa cttagtgcga acccggcaga gactgttgtt gagggaccgt
     2821 ttagtaaaga cgtaatcctc aaaatcacct at
//
LOCUS       DAZOYL010000370         2832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZOYL010000370 DAZOYL010000000
VERSION     DAZOYL010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.373"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            151..1317
                     /gene="nhaA"
                     /locus_tag="ACHUOY_003864"
     CDS             151..1317
                     /gene="nhaA"
                     /locus_tag="ACHUOY_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR0684495.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1383..2282
                     /gene="nhaR"
                     /locus_tag="ACHUOY_003865"
     CDS             1383..2282
                     /gene="nhaR"
                     /locus_tag="ACHUOY_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR0684496.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2320..>2832)
                     /locus_tag="ACHUOY_003866"
     CDS             complement(2320..>2832)
                     /locus_tag="ACHUOY_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0684497.1"
                     /translation="EVSSCTGNNTNCNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRF
                     VLSRINNENYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENS
                     EDGAGQVGFQPSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIM
                     SRVKIVVMYN"
BASE COUNT          677 a          625 c          704 g          826 t
ORIGIN      
        1 ccgtcaaaaa ctcatctcac cgctgatggc gcaaatcctt taattgctcg taaaaaacga
       61 gttattccta cactataatc tgattttaac gatgatttgt gcggggtaaa atagtaaaaa
      121 cgatctattc acctgaaaga gaaataaaaa gtgaaacatc tgcatcgatt ctttagcagt
      181 gatgcctcgg gaggcattat tcttatcatt gccgctatcc tggcgatgat gatggccaac
      241 agcggcgcaa ccagtggatg gtatcacgac tttctggaga cgccggttca gctccgggtt
      301 ggttcactcg aaatcaacaa aaacatgctg ttatggataa atgacgcgct gatggcggta
      361 tttttcctgt tagtcggtct ggaagttaaa cgtgaactga tgcaaggatc gctagccagc
      421 ttacgccagg ccgcatttcc ggttatcgcc gctattggtg ggatgattgt tccggcatta
      481 ctctatctgg cctttaacta tgccgatccg attacccgcg aagggtgggc gatcccggcg
      541 gctactgaca ttgcctttgc acttggtgtg ctggcgctgt tgggaagtcg tgttccgtta
      601 gcactgaaga tctttttgat ggctctggct attatcgacg atcttggggc catcattatc
      661 atcgcattgt tctacactaa tgacttatcg atggcctctc ttggcgtcgc ggctgtagca
      721 attgcggtac tcgcggtatt gaatctgtgt ggtgtacgcc gcacgggcgt ctatattctt
      781 gttggcgtgg tgttgtggac tgcggtgttg aaatcggggg ttcacgcaac cctggcgggg
      841 gtaattgtcg gcttctttat tcctttgaaa gagaagcatg ggcgttctcc agcgaagcga
      901 ctggagcatg tattgcaccc gtgggtggcg tatctgattt taccgctgtt tgcatttgct
      961 aatgctggcg tttcactgca aggcgtcacg ctggatggct tgacctccat tctgccattg
     1021 gggatcatcg ctggcttgct gattggcaaa ccgctgggga ttagtctgtt ctgctggttg
     1081 gcgctgcgtt tgaaactggc gcatctgcct gagggaacga cttatcagca aattatggcg
     1141 gtggggatcc tgtgcggtat cggttttact atgtctatct ttattgccag cctggccttt
     1201 ggtagcgtag atccagaact gattaactgg gcgaaactcg gtatcctggt cggttctatc
     1261 tcttcggcgg taattggata cagctggtta cgcgttcgtt tgcgtccatc agtttgacag
     1321 gacggtttac cggggagcca taaacggctc ccttttcatt gttatcaggg agagaaatga
     1381 gcatgtctca tatcaattac aaccacttgt attacttctg gcatgtctat aaagaaggtt
     1441 ccgtggttgg cgcagcggag gcgctttatt taactccaca aaccattacc ggacagattc
     1501 gagcgctgga agagcgcctg caaggcaaat tatttaaacg caagggacgt ggtctcgaac
     1561 ccagcgagct gggagaactg gtctatcgct atgccgataa aatgttcacc ttaagccagg
     1621 aaatgctgga tattgtgaac tatcgcaaag aatccaattt attgtttgac gttggcgtgg
     1681 ctgatgcact ttccaaacgc ctggtcagta gcgtacttaa cgccgcagtg gtagaaggcg
     1741 agcccattca tcttcgctgc ttcgaatcca cccacgaaat gctgctggag caattaagtc
     1801 agcataaact ggatatgatc atttctgact gtccgataga ctctacgcag caggaaggcc
     1861 tgttctccgt gagaattggc gaatgtggcg tgagtttctg gtgtacaaat ccaccaccag
     1921 aaaaatcgtt cccggcttgt ctggaagaac ggcgactttt gattcctggg cgacgttcaa
     1981 tgttagggcg caaattgctt aactggttta actcccaggg attaaacgta gaaatcctcg
     2041 gcgagtttga tgatgccgcg ttgatgaaag cttttggtgc gatgcacaat gcaatcttcg
     2101 ttgccccaac gctttatgca tatgactttt atgccgataa aactgtcgta gaaattggtc
     2161 gcgtcgagaa tgtgatggaa gagtaccatg ctatttttgc tgagcggatg attcagcacc
     2221 cggcggtaca gcgaatctgc aatacggatt attctgcgct ttttagtcca gcggcgcgtt
     2281 aatcggcacc tcccccaaag ttaaggtggg ggagatagat tagttgtaca taaccacgat
     2341 tttgactcgg ctcattattt gcccgcttga gacattattt ccatatgtac gcgggcgaat
     2401 aaatagagga atctgattac ttccttcatg gggatgctga aaagagtagt aattgctttt
     2461 accatactgg atttcattcg taccatcatt ggagggttga aagcctactt gacccgcacc
     2521 gtcctcactg ttttctaact cattttttaa tatgctggtt cctgactcct gtatcgtcac
     2581 tggttcaaag taatattcaa catcactggg ttcaaaaact aattcacctt gctcgccctg
     2641 gatgcagtca aatcttatat gatgaaactg ataattttca ttattgatac gagacagtac
     2701 aaatcgtcca gtgtcgatgg attgatcttc aaaaccacag gtcattaaat ttacctgtaa
     2761 aatagtattt aaattataat taaagcggac gctattacaa ttagtattat tccctgtaca
     2821 tgaactcact tc
//
LOCUS       DAZOYL010000371         2808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZOYL010000371 DAZOYL010000000
VERSION     DAZOYL010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.374"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..2248)
                     /gene="katE"
                     /locus_tag="ACHUOY_003867"
     CDS             complement(<1..2248)
                     /gene="katE"
                     /locus_tag="ACHUOY_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR0684498.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKID"
     gene            2431..2694
                     /gene="cedA"
                     /locus_tag="ACHUOY_003868"
     CDS             2431..2694
                     /gene="cedA"
                     /locus_tag="ACHUOY_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR0684499.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT          667 a          689 c          731 g          721 t
ORIGIN      
        1 tgtcaatctt aggaatgcgt gaccacacgc ggtgtgctgc catcagcgtt agcagttcat
       61 ccataaaact accgtcagcg ctgtcagctt ccacaatccc ttcttcaccc tggtcagcga
      121 ccttgattgt tgctttaaac ttgcgcgcgt ctcccgccag cgcaatcggt ttaaggtgtt
      181 tgtaggcttc catcaggtag tagttggcat cgccgttgtc agcgatatcc gcgatattgc
      241 cgcaagggac aatgaccgca tcgaccgtca gcgaaggtgc accggcaaag gtagcggcta
      301 taggcaacac cgtaccgtca tccgcagtca cttcacccat tcgggagtag agcagtttgg
      361 catgaacgcc tttggccttc agggccttga gaatggccag aaggtctgcc gatctcactt
      421 catcattaag taaaatcgct accacgcgac ctttcacatc accgtcagga atggcgtaca
      481 aacttaagga tggatccttt ttcagaccgt tgacgtccgg aggtggggtg atattcagct
      541 ggtcgtcagt cagttcgata ccgagatttt tcgccaccgc ctgggccaga gtgagatcaa
      601 tatgcgccag ctggtcaaca acgcgctcac gaatatacgg acgaacgact ttgcttaact
      661 caaaactgaa accatcgaca atatggcgct gctcgaatgg cgtctgactt agccagaaca
      721 gacgcggatg ggaataatat tcgccaaacg atggactgcg ctcacgaact ttatttcctt
      781 ccacgcgttc ctgatatgat tcaaaaccgc cgcgtttcgg tcccggcggt gtttcgcgcg
      841 gccagttatc gttgatcgag ttcggttcgt aattcgccgg gttagtgtcg atccccatac
      901 gatgcatgcc atcacgctgg aagttgtggt aagggcaggt cggacggtta atcggaatct
      961 catggaaatt cggcccacca agacgactga tttgtgtatc ggtataggag aacaaacgtc
     1021 cctgcaacag cggatcgttg gtgaagtcca gaccaggaac aatatgacct ggatggaatg
     1081 ctgcttgttc gttttcggca aagaagttat ccggattgcg attgagcacc attttgccga
     1141 cacgctgaac gggcaccagt tcttccggga tcagtttggt tggatcgaga agatcgaagt
     1201 cgaacttgaa ttcatcttct tcaggaatca actggaagcc cagttcgtat tccggaaaat
     1261 cgcctgcttc aatggcttcc cacaactcgc ggcggtggaa gtccgggtca cgtccggtga
     1321 gtttttgtgc ttcatcccaa acgagtgagg ctttacctgc cagtggtttc cagtggaaac
     1381 gtacaaacgt tgccttccct tcggcattaa tcaggcggaa ggtgtgaata ccgaagcctt
     1441 ccatggtgcg gtaactgcgg gggatgccgc gatccgacat cgcccacatc acgttgtgca
     1501 gagtttcagg ttgcagagaa acataatccc agaaagtatc gtgggcactt tgcccttgtg
     1561 gaattgccca gtgcggttct ggttttaccg catgaacaaa atcggggaat ttatgcgcat
     1621 cctggataaa gaagattggc gtgttattgc caacgaggtc aaaaataccc tcttcagtat
     1681 agaacttggt ggcaaaacca cggatatcac gcacggtatc tgcagagcca gcgccaccct
     1741 gaacggtaga gaacctaaca aatactgggg tgattttgtt cggatctgag aggaaatccg
     1801 ctttggtgat atcgcttaag cttttatatg gctggaaata accgtgagct gctgatccgc
     1861 gtgcatgaac aatacgttcc gggatgcgct catggtcaaa gtgggtgatt ttctcgcgca
     1921 gaataaaatc ttccagcagc gttggaccac ggttaccggc acgcagtgag ttttgatcat
     1981 cggcgatgcg cacgccctga ttagtggtca gcgcataatt ttcactgcct ttgcgtacgt
     2041 cttccagaga attaagtttt tcgttacgtg tatcaggggc tttcaggctc ccaggggcgg
     2101 taggttgtgc acctggcggt gttggttcag ccgctggacg atgagatccg tcctcaggtg
     2161 ccagtgagtc catccccggt ttcgcttcgc tggaatcgtg tagtggtgac tggtgctgat
     2221 gtaggttctt ttcgttatgt tgcgacattg aactcgtctc cttaatttat tactgaaagg
     2281 gccgctgttt tggtcaaaaa actctctaac tatagaccac cagccagatc ccgcttcgga
     2341 gactattctg gaaacggtgt tatgaaatac acgcaaagcg ggacgtacag gggctaaatc
     2401 ggctaaacta cagtttttct aatttttaat atgcgtttag tgaagccagt aatgaagaaa
     2461 ccgctccgcc agcaaaaccg ccagattatt agctatgtcc cacgcacgga acccgcgccg
     2521 ccagaacatg cgataaagat ggattcgttt cgtgatgtct ggatgctgcg tggcaaatat
     2581 gttgcgtttg tactgatggg agagtcattt ctgcgctcac cggcgtttac tgtgcctgaa
     2641 tctgcccaac gttgggcaaa tcagatccgc caggaagggg aagtgactga gtaaggatta
     2701 catacttccc ggttttgccg ggagttacat gacctgaaat tctgcatttc gttgctacat
     2761 tcattcaaac cacctgtaac aaaaagctgc ctttgacgag ctattgct
//
LOCUS       DAZOYL010000372         2739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZOYL010000372 DAZOYL010000000
VERSION     DAZOYL010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.376"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(101..1174)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUOY_003869"
     CDS             complement(101..1174)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUOY_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR0684500.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(1185..>2739)
                     /gene="lpfC-O113"
                     /locus_tag="ACHUOY_003870"
     CDS             complement(1185..>2739)
                     /gene="lpfC-O113"
                     /locus_tag="ACHUOY_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0684501.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT          759 a          646 c          546 g          788 t
ORIGIN      
        1 tgaaaatccc acatgtcatc tttttgtcat gatgcattta aaagtgataa agaaatgggt
       61 gagatgacaa cgatgtgatg aacaatcgtt gtcagaccta ttagcgcaca atctcaacag
      121 ttatcgtcgc tgttgcagta aattgccccg gggctgggcg agctccagta aggctcgcag
      181 gagcagctga aaaagttaca ctacctgatt tttcaccata cactgttgat ttacccatgt
      241 ctgttggcaa ctcaccaccg ttcactttga tttccttatt atttttgtcg taaattttta
      301 tccccacgtt agagtttgaa gttacgacca ttgaattgtc cgtattgggt tcggtagcat
      361 aaaaagacgt actgatatca tcggtattca gtatccccag gttttcacac tcaatagtga
      421 ctgttttagt aattttatgt gttgttaccg cacttccaac aacagatgaa aaatctgcag
      481 ctggtatttc tttaaaatca aaataaactg gctgtaaatt atttatttcg caactttgcg
      541 gagctgtaat atcgccccga atataaacat cagccatcgg ctgtgagtaa tccaccgggg
      601 tgtcacggct aattgttcca tacaatgtca ctatctttgt ttcaggaata atcaattttc
      661 ctgcaaatgt cttcttgata taaaaatata ctatcgcttt acccccactg gatatactta
      721 aaggttcaat gcggttgcaa ttgtactttg tattaccaga cgggttgtta gcatgatatt
      781 caaaaggtgc attcacatac ccaaccccaa gaatatctat agcaatccca acatcaaggt
      841 tcggattaag atcataataa ttaatattag agtgaacccc agcataagtt aatgcctggt
      901 ttatttttga cgtataatag atatagggga attctcctac attaccagta tcacaatgac
      961 agcgtccagg atatgaggat ccattagtta cagtggtatt aaatgtcgtt ccaaccttat
     1021 tttcgttcgc ggtaataaga tatcccgaga aatcaagcgt acggtgaaat acgccattat
     1081 caggggtaca gataccttta ggagctgcaa gtgctacact acttaacgag agcataaaca
     1141 cagcaaagca ccactgtttg atatacttgt tcataaaaat ccttttaatg acatgtcaca
     1201 ccctgttcaa ttaatccgct ctcactttct ggtgtcgaaa gtgaataatt gaagatgcat
     1261 tttgttgagg cgttttctcc ccaggataac gtaacctgtc ctttttctgg tagaccagaa
     1321 agataaagtt cgccacggtc accgacaatt cctgaatctg ccaaacttgc atcttgtatc
     1381 gcagcaatgg ctccaaaagg taccggttta ccatttgcat ttaacactct gaagagaacg
     1441 cgataaccca catgagtcac aaactccgct ttaaccactg caccacgcgt gggaaccatc
     1501 gtaacgacgg tattatcaag ttctatattc gacttcgaga aatagttatc gctgattgct
     1561 acccgatttt catcataagg gtttagctgt gtcttgaccg tatagccgcg ccagtccgtc
     1621 gcagcaccgc gcatattatc tatttctaac ccggcggccc caggtgcttt aacaagagca
     1681 atagtttcac ctaactcctg acttaacgtt accccttcgc tgtgtgccag aattccccct
     1741 ctgataccat aattcagcct ctgggaattt tggctgtagt tatagctgcc gttcaccgtg
     1801 ccataagagc catcataggt cagggacata ttaccgccaa cctcatcatc atgactgtta
     1861 taggactgca ttaaattcca tgaaaggtta tttctttcca gcgccgtacc gctgagtccg
     1921 agttgatgtg agctaccgga attatcgttt tgcgaaacag aataggtggc gtaactatta
     1981 ccccaccagc ccaataaagg aacagataaa gagagcatgt agatctgctc actggtgtta
     2041 tcactggcat ggttgttccc cgtcccctgt ctggcagtat tggaataact ccaggagacg
     2101 ttccacgaga gacgcttcca gctactgctg tagccaaatt gcattaatcg ttcgacatca
     2161 tcggtatgcc agtattgttg ccggcttaat gtcaggtata aattgccata atctctcccc
     2221 aacgactgcg ttagcgtccc ctccacgcaa ctacgtcggt tacctgtttc ccaaaagtct
     2281 tcagggctgt tgcgccgcga tgcccactca ttgagggtat agtagccctc ggtggagtaa
     2341 cgatagccaa ccaagcgcaa gctagtgtct gtgtcgtcaa atcgttttga atagagaaag
     2401 cgatatgact gacccgtttc tgtatcatca tggctaaaat tcgcacgagc atgtgtcaca
     2461 tcgaaagaaa ttgcgcccaa agcgccaaga tctttaccga cacccagagc tgcagaacca
     2521 taattttcag cagcctgcat acctccatat aaagtgatcc cgtgggaaaa accatgaagt
     2581 atttgcgcct gaaggacgtc cggtgtaaat ccgtcttcat cgcgcaattc acccacgctg
     2641 acatcaacat ctgtctgacc ttcacgttta agaatcgcta atgaagcata aggttgggtg
     2701 aatcgttgtt cgctaccgtc ttcttctttg atggttacg
//
LOCUS       DAZOYL010000373         2736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZOYL010000373 DAZOYL010000000
VERSION     DAZOYL010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.377"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..522
                     /gene="fliD"
                     /locus_tag="ACHUOY_003871"
     CDS             <1..522
                     /gene="fliD"
                     /locus_tag="ACHUOY_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0684502.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
     gene            537..947
                     /gene="fliS"
                     /locus_tag="ACHUOY_003872"
     CDS             537..947
                     /gene="fliS"
                     /locus_tag="ACHUOY_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR0684503.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            947..1312
                     /gene="fliT"
                     /locus_tag="ACHUOY_003873"
     CDS             947..1312
                     /gene="fliT"
                     /locus_tag="ACHUOY_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR0684504.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            1390..>2736
                     /gene="amyA"
                     /locus_tag="ACHUOY_003874"
     CDS             1390..>2736
                     /gene="amyA"
                     /locus_tag="ACHUOY_003874"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0684505.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHL"
BASE COUNT          762 a          632 c          702 g          640 t
ORIGIN      
        1 tataaagaag tagacccagg tgctgaagca caagataaaa ataatggtgc acttctcgga
       61 gacagcgttg tacgtacaat tcaaagcggc atccgcgcac aatttgcaaa cggtgccagc
      121 gacagtgcct tcaaaacatt aaacgagatc gggattaagc aggacggcac gacgggtaag
      181 ctgaaaattg atgatgataa actcaaaaaa gtcctgaatg aaaatacagc atccgtacgt
      241 gaattgctgg taggcgatgg taaagaaaca ggaattacca ccaaaattgc caccgaggta
      301 aaaggttacc ttgccgatga tggcattatt gatagcgcac aggacagtat taacgccaca
      361 ctgaaaaaac tgaccaaaca gtatctatcc gtcagtgcca gcattgatga caccgtggcg
      421 cgttacactg cacagtttac gcaattagat accatgatga gcaaactgaa taacaccagt
      481 acctatctca gccaacaatt tactgcaatg agtaattcct gatcacggag gtcagcatgt
      541 ataccgcgag cggctcaaaa gcctatgcac aaattggcgt cgaaagcgcc gtaatgagcg
      601 ccagccagca acagctggtc accatgctat ttgatggagt gctgagcgca ctggttagag
      661 cgcgcctgtt tatgcaggac aacaatcagc aaggcaaagg cgtctctttg tcaaaagcga
      721 tcaacatcat tgagaacggg ctgcgggtga gtcttgatga agagagcaaa gacgaactaa
      781 cccaaaactt gattgctctt tatagctata tggtcaggcg cttgctgcaa gccaatttac
      841 gcaacgatgt ctccgcagtc gaagaagtgg aagcattaat gcgcaatatt gccgatgcct
      901 ggaaagagtc tttactctcc ccttctttga ttcaggaccc agtctgatga accatgcacc
      961 gcatttgtat ttcgcctggc aacaactcgt cgataaaagc cagctcatgt tacgcctggc
     1021 aacggaagaa caatgggacg aactcatcgc cagcgaaatg gcgtatgtga atgcggtgca
     1081 ggagattgca catctgactg aagaggttgc cccgtccacc acgatgcagg agcagctccg
     1141 cccgatgctg cgcctgattc tcgacaacga aagcaaggta aagcagttat tacagattcg
     1201 tatggatgaa ctggcgaaac tggtcggtca gtcatcggtg caaaaatcag tgttaagtgc
     1261 ctatggagat cagggcggct ttgtgctggc tccgcaggat aacctctttt gaatcttaac
     1321 gagccgatgg ctcgcgaata atccgattac ggctacgctt ctaatgttcc ccttgaatgg
     1381 agtcgaagaa tgcgtaatcc cacgctgtta caatgttttc actggtatta cccggaaggc
     1441 ggtaagctct ggcctgaact ggccgagcgc gccgacggtt ttaatgatat tggtatcaat
     1501 atggtctggt tgccgcccgc ctataaaggc gcatcgggcg ggtattcggt cggctacgac
     1561 tcctatgatt tatttgattt aggtgagttt gatcagaaag gcagcatccc tactaaatat
     1621 ggcgataaag cacaactgct ggccgccatt gacgctctga aacgtaatga cattgcggtg
     1681 ctactggacg tggtagtcaa ccacaaaatg ggggcggatg aaaaagaagc tattcgcgtg
     1741 cagcgtgtaa atgctgatga ccgtacgcaa attgatgaag aaatcattga gtgcgaaggc
     1801 tggacgcgtt acaccttccc cgcccgtgcc gggcaatact cgcagtttat ctgggacttc
     1861 aaatgtttta gcggtatcga ccatatcgaa aatcctgatg aagatggcat atttaaaatt
     1921 gttaacgact acaccggcga aggctggaac gatcaggttg atgatgaact cggtaatttc
     1981 gattatctga tgggtgagaa tatcgatttc cgcaatcatg ccgtgacgga agagattaaa
     2041 tactgggcgc gctgggtgat ggaacaaaca caatgcgacg gttttcgtct tgatgcggtt
     2101 aaacatattc cggcgtggtt ttataaagag tggatcgaac acgtacagga agttgcgcca
     2161 aagccgctgt ttattgtggc agagtactgg tcgcatgaag tcgataaact gcaaacgtat
     2221 attgatcagg tggaaggcaa aaccatgctg ttcgatgcgc cgctgcagat gaaattccat
     2281 gaagcatcgc gcatgggacg cgactacgac atgacacaga ttttcacggg tacattagtg
     2341 gaagccgatc ctttccacgc cgtaaccctc gtagccaatc acgacaccca accgttgcaa
     2401 gccctcgaag cgccggtcga accgtggttt aaaccgctgg cgtatgctct aattttgttg
     2461 cgggaaaatg gcgttccttc ggtgttctat ccggacctct acggtgcgca ttacgaagat
     2521 gtcggtggtg acgggcaaac ctatccgata gatatgccaa taatcgaaca gcttgatgag
     2581 ttaattctcg cccgtcagcg tttcgcccac ggtgtacaga cgttattttt cgaccatccg
     2641 aactgcattg cctttagccg cagtggcact gacgaatatc ccggctgcgt ggtggtcatg
     2701 tcgaacgggg atgatggcga aaaaaccatt catctg
//
LOCUS       DAZOYL010000374         2733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZOYL010000374 DAZOYL010000000
VERSION     DAZOYL010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.378"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(14..>2733)
                     /locus_tag="ACHUOY_003875"
     CDS             complement(14..>2733)
                     /locus_tag="ACHUOY_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001745227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0684506.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRV
                     WRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETEN
                     GEQAKARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTA
                     EQMAAQLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQH
                     LQQSLRLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFI
                     DSLGLKFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSN
                     NMTIYWNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYE
                     NKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
BASE COUNT          489 a          886 c          683 g          675 t
ORIGIN      
        1 ataaaaaacc tcactattct ggcgtacccg ttattttcat cgtctgcaac ggtccatcca
       61 cccgataaaa aggcagtcca ctatgagctc ctctggtaca ctcacctaaa gttttcgccg
      121 cacgcgattc aattattgta ataacccttg cctcttcttt attctcatat ttcttatctg
      181 ttcggcttaa aagagccatg aaattttcct tatcaagaag gcaatactgc gcatgagtaa
      241 actcatgtcc caaacctaat gcaggcgatt gtgatttcga gtttaattca gttgaacaaa
      301 ataaagatgc tctactattc caatatatcg tcatattatt cgaattaaat ctaacattgg
      361 ttccttcaat atactctata tttatagttt cttcagagct tgataaaaaa tctatagttt
      421 ctttcatttg tgaatcttgt tttaaatact caacagcctg attaaaatca cttggatcac
      481 cgttaacatg gaattttaaa cccaaagaat ctataaaccg aacagggttt aatggatatt
      541 tatacagatt ccattcacct ctcagcccta tcggatcctg ggtgatatac cttccctgca
      601 acgggtcata gtaccggttg cggttgtagt acagccccga ctcctcatca tactgctgcc
      661 ccggcagacg gagtgactgc tggaggtgct gcgcgctggt ttcgcccagc aggtttcccc
      721 attcgtcata ctccccctgc cacgccgttt ccccttccgg gctgatgagc gccagcggca
      781 gtccccggtg gtcgcagtgg taaagatgaa gtttcctctc cgggatgtat tccgcctcca
      841 gctgcgcggc catctgctcc gccgtcagcc cgcactgcgc aagccatgcc tcactctccg
      901 cactcaccgc tcccgcgcgc agttcccgct ccagcctccc cagcatcacc gacagctccg
      961 ccggtagcgt cacacccgtg tcctcctgca acacctccgc caggctccgg tgccgcgcct
     1021 tcgcctgttc gccattctct gtttcgattc tcaggagcgg cgtgaagctc cccggctggt
     1081 ataccgtctg gatacgcgtg gtgccggtct gtatcgtcgt cagcctgtca ccgtcccacc
     1141 cgtaccatgt cacctccggt ttacgcgaca gcgacatcca ccccgtcagg tcacgctccc
     1201 gccgccatac ccgttttccc gtccggcggc ccagcgggtc atagaggtac cggctctcca
     1261 cctgtggttc gccgtgctgt atccgcgtgt agaacaccag gcggtgctgg ctgtcgtagt
     1321 gatagtggtg ggtgcgctcg tcgtgcatcc ggataacccc ttccgggata cggtccgtct
     1381 tctccgccag cctgccgtat tcatcgtagc gatagacata gtgcgcatct tccgcgatgc
     1441 ggttatccgg ccacgccgtg agggtgctgt ccgggtgcag ttccgggtcc ggcagccgat
     1501 ttcctgccgg gtctgttgca tacgggatat cgatatccag gttcgctgcc gtggtgtgga
     1561 cgcccgtcag cctccccgtg tcgctgtaac ggtactcccg gctctcctgc gggccactga
     1621 tgcggattag ctgtccgttg tcgttccagt cgtagtcacg gtcaagctgc ggaaggttca
     1681 ggtgcctgct ccggagctgg ccgctggtat tccaggcggt ggcaagttca tatgcctccc
     1741 cgccgaagct gcgggccgtc tcacggtgca gccggtcccg cgtgtactcg accagcggtg
     1801 ttccgcccag cttcatcccc gcaagataac cgctgccata cgtcagccac tccaccggcg
     1861 gcaggccgtc cggctcctga cgggtcgcca gcccctgttc gctgtacgcg tgccccgtct
     1921 catgctccca cagcatctcc cccgtctccg gggtctccac cgtctgccgt tcgcccgtca
     1981 gacggccttt atcgtcatag ccgtagtgga ccgccacacg gtggccttca ctcaggtggc
     2041 tgatttctgt cagccagccg tggtcgtcat actgccactg ctccgccggt tcaccgttca
     2101 ccgtgcgccg ggtgatacgg tccgacgcat cgtaatgcca gagggtgata agcccctcat
     2161 cttcactctg cgtgagtttt ccggtcaggt catactggta acgttgcgtc cggccgtcaa
     2221 agccgcgctg ctccgtcagc ctgtcgaccg ggtcataccg gaacgtggac tggctgccgt
     2281 tctcgttggt cagcacagtg atgcgcccgg cggcgtcata ccccatgctt cgcgtcagac
     2341 cgccctgcgt ggtgctgacg gcctttcccc acgcatcata ctgtatctca ctgcggctgc
     2401 cgtccggggc aacgatagcg gtgaggtctc ctgcggcgct gtactcataa cgggtttcac
     2461 gcccctgcgc atccctctga ctgaccagtt gaccgcgcgg gttataactg ctgtaagtgc
     2521 tgatgccttc ttcccggtga acggcgattt gttgaccgta ccggtcatac tcataccgcg
     2581 tcgtgtaccc cgagcagtcc gtaaacgcct gaagctgacc gtaccggttt cgcgttattc
     2641 tccgcctcac cccggttccg tcttccaccg cacagggcag gccactgcgg gaagaatcat
     2701 aaaaccagcg cgtaatgtta ccgttgcgcg agg
//
LOCUS       DAZOYL010000375         2729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZOYL010000375 DAZOYL010000000
VERSION     DAZOYL010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.379"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..474)
                     /gene="dfrA1"
                     /locus_tag="ACHUOY_003876"
     CDS             complement(1..474)
                     /gene="dfrA1"
                     /locus_tag="ACHUOY_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0684507.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            806..1783
                     /gene="intI2"
                     /locus_tag="ACHUOY_003877"
                     /pseudo
     CDS             806..1783
                     /gene="intI2"
                     /locus_tag="ACHUOY_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(1971..2210)
                     /locus_tag="ACHUOY_003878"
     CDS             complement(1971..2210)
                     /locus_tag="ACHUOY_003878"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0684508.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          786 a          585 c          551 g          807 t
ORIGIN      
        1 ttaacccttt tgccagattt ggtaactata atttatgtta gaggcgaagt cttgggtaaa
       61 aactggccta aaattgctgg ggatttcagg aaagtaaaca tcaccttccg gctcgatgtc
      121 tattctagat atatgtagtg tatctacttg atcgatcagg cttttgtata tctccccacc
      181 acctgaaaca atgacatgat ccgttatttt ctttaggttg gttaaagcat ctttaattga
      241 tggaaagatc aatacgttct cattgtcaga tgtaaaactt gaacgtgtta cgaccgcata
      301 ctttcggttg ggtaatgctc ccattgattc aaaagtcttg cgtccaacca acagccattg
      361 gttataggta atagctttaa acaggagctg ttcacctttg gcactccatg gaatatcagg
      421 gccattcccg ataactccat tcttcgatat agctaccatt agtgatagtt tcacaattct
      481 tcctcagagg ttaacatttt attagtaagc atgctcgttt tgtcacccgc tgatgcttac
      541 cgttaattaa tataatcact tgtattaaat agactttctt taaaaataca agacactctg
      601 ttattacaaa tcgtgcatgc cgtctatcct gcttgcacga tgcacattat ctcacccaaa
      661 gaactcataa cattttaatt tatttagcat taaattaaaa ctctaccggt aaacaagcat
      721 ctctaggcgt aaaaaacagg cactttttta aatcccagct agaataggct gtataggcag
      781 acagttgcaa gacaaggagc gttttatgtc taacagtcca tttttaaatt ctatacgcac
      841 ggatatgcga caaaaaggtt atgcgctgaa aactgaaaaa acttacctgc actggattaa
      901 gcgttttatt ctgtttcaca aaaaacgtca tcctcagacc atgggcagtg aagaggtcag
      961 gctgttttta tccagcttag caaacagcag acatgtagcc ataaacacgc agaaaatcgc
     1021 tttaaatgcc ctagcttttt tgtacaacag gtttttacaa cagccgttgg gcgatattga
     1081 ttatatccct gcaagcaagc ctagacggct accctctgtt atctctgcaa atgaagtgca
     1141 acgcattttg caggttatgg atactcgcaa ccaagttatt tttacgctgc tgtatggtgc
     1201 aggtttgcgc attaatgaat gcttgcgttt gcgggttaaa gattttgatt ttgataatgg
     1261 ctgcatcact gtgcatgacg gtaagggtgg gaaaagcaga aacagcctac tgcccacgcg
     1321 cctaatccca gcaataaaat aactcattga gcaagcgcgg cttattcagc aagacgacaa
     1381 cttacaaggc gtagggccat cgctgccttt tgctttagat cacaaatacc cttctgctta
     1441 tcgacaagcg gcgtggatgt ttgtctttcc ctccagcacg ctctgcaacc acccgtataa
     1501 cggcaaatta tgccgccatc atctgcatga ctccgttgcg cgaaaggcat tgaaggcagc
     1561 cgtacaaaaa gcaggcatcg ttagcaagcg tgtcacttgt catacatttc gtcactcgtt
     1621 tgctacgcat ctattacaag cggggcgtga tattcgcact gtgcaagaac tcttagggca
     1681 taacgatgtt aagaccacgc aaatctatac gcatgtgttg ggtcagcatt ttgccggcac
     1741 caccagtcct gcggatggac tgatgctact tatcaatcag taaaagtaga taaatatctt
     1801 tatgtagccc tgagtagccc ctgatacttt ttatggtgaa aaagataaac aatcatagag
     1861 gggataaata tgagccagtt ctaccattgt tcagataagt ttaagcaaga tactgtcaat
     1921 cacagtgacc attcatggtc attacacttc ctccagttgc cagacaatgc ctaacataaa
     1981 aacgcaaaag cccaaaagtc agagcttagg tgttctaggc gttttctaag cgctgaagaa
     2041 atacgtctta ccgtagattg cgtgcgtttc tgaagcacat tttccttata catggcttta
     2101 tcccaggcag cttcatcaag ctcttggagt aacaagccag taataatgcg acactctcgg
     2161 gtttgtagcg agccgctggt gagatcggag ttatagcgga aaggggtcat ttgtaattat
     2221 ttgtgggtgg aggatagtta acttattatt gtagttggaa gaggctgctt tccaaacatt
     2281 agcacctcgt aatgcttgtt actgtatatt aatttatgct cttagcgcat gttaatgggc
     2341 tattacaatg cataatgtcc acatcaagat aactgtggct taacacctgt acaagcgaaa
     2401 aaaatcaatc acctgtccgg aaatacggga tcactgtaaa gtgttggtga ggcagtctag
     2461 gagggggatt taatttggga tactgatgaa aggattatga tcagttggat ataggatcgt
     2521 actaagctga gcggcaggtt agctagatta gttaaccaga taagtgaaat ctagttccaa
     2581 actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta
     2641 ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca
     2701 actattttgt ccgcccacag ggctgccga
//
LOCUS       DAZOYL010000376         2727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZOYL010000376 DAZOYL010000000
VERSION     DAZOYL010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.380"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHUOY_003879"
     CDS             complement(423..680)
                     /locus_tag="ACHUOY_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684509.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHUOY_003880"
     CDS             complement(677..1174)
                     /locus_tag="ACHUOY_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR0684510.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHUOY_003881"
     CDS             complement(1376..1834)
                     /locus_tag="ACHUOY_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0684511.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHUOY_003882"
     CDS             complement(1831..2328)
                     /locus_tag="ACHUOY_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0684512.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUOY_003883"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUOY_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR0684513.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          708 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataacc
//
LOCUS       DAZOYL010000377         2721 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZOYL010000377 DAZOYL010000000
VERSION     DAZOYL010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.381"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            30..2546
                     /locus_tag="ACHUOY_003884"
     CDS             30..2546
                     /locus_tag="ACHUOY_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0684514.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2721
                     /locus_tag="ACHUOY_003885"
     CDS             2557..>2721
                     /locus_tag="ACHUOY_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0684515.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          656 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga g
//
LOCUS       DAZOYL010000378         2702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZOYL010000378 DAZOYL010000000
VERSION     DAZOYL010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.382"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            26..772
                     /gene="mipA"
                     /locus_tag="ACHUOY_003886"
     CDS             26..772
                     /gene="mipA"
                     /locus_tag="ACHUOY_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR0684516.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            862..1716
                     /gene="yeaE"
                     /locus_tag="ACHUOY_003887"
     CDS             862..1716
                     /gene="yeaE"
                     /locus_tag="ACHUOY_003887"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR0684517.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1767..2651)
                     /locus_tag="ACHUOY_003888"
     CDS             complement(1767..2651)
                     /locus_tag="ACHUOY_003888"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR0684518.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
BASE COUNT          683 a          659 c          697 g          663 t
ORIGIN      
        1 acatttgttt aaggaatgat taattgtgac caaactcaaa cttctggcac ttggagtgct
       61 tatcgcaacg tctgcaggcg tagcgcacgc tgaaggtaaa ttttccctgg gcgcaggcgt
      121 cggtgtcgtt gaacacccat ataaagatta cgataccgat gtttacccag taccggtaat
      181 caactatgaa ggcgataact tctggttccg tggcttaggt ggtggttact acctgtggaa
      241 tgacgcaacg gacaaacttt caattaccgc ttattggtcg ccgctttact tcaaagcgaa
      301 agacagtggc gatcaccaaa tgcgtcacct ggatgaccgt aagagcacca tgatggctgg
      361 tctgtcttat gctcacttta cccagtacgg ttacctgcgt accaccctga ctggcgatac
      421 cctggataac agcaacggca ttgtctggga tatggcctgg ttgtatcgtt acaccaacgg
      481 cggcctgaca gtgaccccgg gtatcggtgt gcagtggaac agcgaaaacc agaacgaata
      541 ctattatggc gtatcgcgca aagagtccgc tcgcagcggt ctgcgtggct ataacccgaa
      601 cgacagctgg agcccttacc tggagctgag cgccagctac aacttcctcg gcgactggag
      661 tgtttacggt accgcgcgct atacccgtct gtctgatgaa gttactgaca gcccgatggt
      721 ggataaatcc tggactggcc tgatttctac cgggatcacc tacaaattct gataatgcat
      781 ctttccaggg cattttgtgc ataaaaacag ggcgtcacgc cctgttttgc attacggtgg
      841 tgcaatacct gggaggaaac aatgcaacaa aaaatgattc aatttagtgg cgatgtctca
      901 ctgccagcca tagggcaggg aacatggtat atgggcgaag atgccagtca gcgcaaaaca
      961 gaagttgctg cactacgcgc gggcattgag ctcggtttaa ccctcattga taccgccgaa
     1021 atgtatgccg atggcggtgc cgaaaaggtg gttggggaag cattaaccgg tctgcgtgat
     1081 aacgtctttc tcgtctctaa agtctatccg tggaatgctg gcgggcaaaa agcgataaat
     1141 gcatgcgaag ccagtttacg ccgtctcaat actgattatc tcgatctcta cttattacac
     1201 aggtctggca gtttcgcttt tgaagagact gttgcagcga tggaaaaatt gatcgcccag
     1261 ggaaaaatcc gccgctgggg cgtttctaac cttgattatg ctgatatgca ggaactctgg
     1321 cagctgccgg ggggaaatca gtgtgccact aatcaggtgc tttaccatct cggttcacga
     1381 gggattgagt acgatctact cccctggtgc cagcaacagc agatgccggt gatggcttac
     1441 agtccattag cccaggccgg gcggttgcgc aatggactgt taaaaaacgc ggtagtcaac
     1501 gaaattgcac atgctcacaa tatcagctcg gcacaagtat tgttggcgtg ggtaatcagc
     1561 catcagggtg tgatggcgat tccaaaagcg gccacgattg cccatgttca acagaatgcg
     1621 gctgtgcttg aggtcgaact ttcgtcagcg gaattaacca tgctggataa agcgtatccg
     1681 gcaccaaaag gaaaaactgc gctggatatg gtgtgatgtt gatacgtgcc ggatgcatcg
     1741 cacccggcac acaacattaa cgcaaattaa cgtttcgcaa cgcgaatgga ttgcgccaga
     1801 tgtgcaggtt tctctttggt gactttttgc gtttctgaag cgtaagccgt ttctacacaa
     1861 acaaatgttt tgtagccatc atccggcata tcgcccatgc taattgaaag cgccggtccc
     1921 gggttccagc ctacaacgtt cagatgatgc tggtggccta cggcgataat acgattcagc
     1981 gcttcatcat taatcacgct gcaatcttgc ggattcagat acacgcgatc ggtacggtca
     2041 gggaaggtct gaataccgtc ggtcagtaca tcttctttcg cgtcattcac tttatcaatg
     2101 aagcgatcgc ccagcccact gacgcttacc ttagcgatat cacccacgtt aaagtaggta
     2161 tgcagggcag aggtggtttc aaattcgcca tgtgattcaa gatcgatttc acaggtttta
     2221 cccacgcgga aatgcgctaa cagcgtaaag tcgtgcggac agaatttttt cgtctcttcg
     2281 ctttgcgtca attcaaaagt cagcgctacg ccatcagcat cttcattatg tgatttcagc
     2341 gtccacggca ggttgcgggc aaaaccgtgc gcaggcagac cttgttgtgc cgccggacca
     2401 aaccacggcc agcaaaccgg tacgccaccg cgaatagcga cgccattttt gaacggtgtg
     2461 ttgttgctca accacagaac ttcttcttca cccgcaggtt tccacgagag aaggtgtgcg
     2521 ccctgtaatg caaaagaggc ttttacttgg ggatgatcga ccacaatgag gtccagttca
     2581 tccagtttac gacgggagag gacaggggag atttgttcga tgaccggaag ggcaaaaatt
     2641 ttcttaatca tgacgcagtc ctttaacttc attttatcag gtaaaaaaaa gagcgaccga
     2701 ag
//
LOCUS       DAZOYL010000379         2692 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZOYL010000379 DAZOYL010000000
VERSION     DAZOYL010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.383"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(4..327)
                     /gene="mobA"
                     /locus_tag="ACHUOY_003889"
     CDS             complement(4..327)
                     /gene="mobA"
                     /locus_tag="ACHUOY_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR0684519.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            613..807
                     /locus_tag="ACHUOY_003890"
     CDS             613..807
                     /locus_tag="ACHUOY_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0684520.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
     gene            1305..1466
                     /locus_tag="ACHUOY_003891"
     CDS             1305..1466
                     /locus_tag="ACHUOY_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0684521.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRL
                     EVMRLLRLN"
     gene            1743..2468
                     /locus_tag="ACHUOY_003892"
     CDS             1743..2468
                     /locus_tag="ACHUOY_003892"
                     /inference="COORDINATES: protein motif:HMM:NF045398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="HFR0684522.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLF
                     QKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIY
                     RKEYATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKV
                     INESGVKYDVIHFGACRVNREEK"
     gene            complement(2489..>2692)
                     /locus_tag="ACHUOY_003893"
     CDS             complement(2489..>2692)
                     /locus_tag="ACHUOY_003893"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684523.1"
                     /translation="FPGRKLLKTDGMNTVSSQSDLHSLSCIAMQRAENDPDASFLFFR
                     GKTSIFVVSASESRSARFSGAYN"
BASE COUNT          859 a          665 c          579 g          589 t
ORIGIN      
        1 aagctaatct gccataagcc tggataacag ggcacggtga tactccgtaa tagcgatcag
       61 cacctccgca tactccctgt ccccgatacg cttgccgtcg ataaagagtt ttttctgtaa
      121 cgcccccagt ctcataactt ctttcagcac ccgatcatca gtcagtgagt taacgttctt
      181 accgagagct gccgcccgta aaaaaccaga aacggtcttg ccgcattcag cagccctttt
      241 tctgatttcc tggtcttctt ctgccgtcag acgaacaggg cgactgatag cccgcctgcg
      301 cgtattactt ccacttcgtt ttgtcataaa acatggctcc gtatctgaca cggggtgtcg
      361 gggcgaagcc ctgaccaggg taattgtaat agcgtgcatg tatgcgcggt ataacaatta
      421 cacatcctgt cctgtcagca agttcgaatt accgacaaag gcacggcagt ttttttggca
      481 gtgacagtat tgtcaggcag aaatacccgt caatgcgtca gacggcagaa taccggcggt
      541 aaaacagtcg aaaagtggta cgcgatataa tcacacaacg cagatgacag acagaacgga
      601 gaaaacagaa agatgaacaa gcagcagcaa accgtactca acatggcggg attcataaaa
      661 agccagagcc tgacgctgct cgaaaagctg gacgcactcg atgccgacga acaggccgcc
      721 atgtgtgaga agctgcacga aatcgcggaa gaactccaga acagcataca gacacgcttt
      781 gaagcggaaa gtggcaccgg ggtataacag tgtatcctgc tccagacagg atgcagaacg
      841 gtataccggt gcgtaaggag aaaatgtcac ataaggcgct cttccgcttc ctcgctcact
      901 gactcgctac gctcggtcgt tcgactgcgg cgagcggtgt cagctcactc aaaggcggta
      961 atgcggttat ccacagaatc aggggataac gccggaaaga acatgtgagc aaaaagccga
     1021 aggcagggac aaaggccgcg caggtggcgt ttttccatag gctccgcccc cctgacgagc
     1081 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1141 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgtttaccg
     1201 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt
     1261 ggtatctcgg cccggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1321 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1381 acgaattatc gccactggca gcagccactg ataaccgaat tagaggagta agacttgaag
     1441 tcatgcgcct gttaaggcta aactgaaaga acaagttttg gtgactgcgc tcctccaagc
     1501 cagttacctc ggctcaaaga gttggtagct ctgagaacct tcgaaaaacc gccttgcagg
     1561 gcggtttttt cgtttacagg cagcagatta cgcgcagaaa aaaaggatct caagaagaac
     1621 ctttaatttt ttttcacact tggtatccta ctttaaccca taaaataaac acttacaccg
     1681 acacaccctt aaacaaaaga aaaaaaacaa taaaaaacac cgcaaaaatg gattgttaac
     1741 aaatgaaaat actcaattcg aatctcataa caaacatatc atcaacaata agaaatcacc
     1801 ttctaacatc atccaaaaat ataaaaacac acccatcacc aaacatggac gtaacaagtc
     1861 atcgtcgttt attccagaaa gacggaatag gattgagcca aacaggaaaa gcaacacaac
     1921 ttgtcattat gtcgcatggt ggatggaaag agatctccca tccacaaaca ctattcacgc
     1981 gtcaaaaagg cgatggatgg actgtagtcc ccaaaaacct aagaattgat ttttatacaa
     2041 aagacaacga cttcacaaag ggactaagcg ttctttcaga ggtaaacaaa cgccatactg
     2101 aagctcaaaa agggttaaca ccatcactaa acataagcaa agatgaccta aaagttctag
     2161 cgaacgcgcg caatatacct caatcaaaat tagaagaaga aatgatgtca caagccatat
     2221 atagaaaaga atacgcaaca agtaatgaga agataaaaaa ctatgctcta tattaccatg
     2281 aacaagccca aaatattata caaagacatc aagatggctt aggaaacaaa aatattgata
     2341 tagcatttat aactgataaa aatcataaga agcacctttc tgacatattt aaagtaataa
     2401 atgaatcagg agtaaaatat gatgtaattc actttggcgc ttgccgagta aatagagagg
     2461 agaaataatc atcatttacc ataatccctt aattgtacgc accactgaaa cgcgctgaac
     2521 gcgattctga cgcagacacc acaaaaatag atgttttacc ccggaaaaac aggaaggatg
     2581 cgtcaggatc gttttcagct cgctgcatag ctatgcatga aagcgaatgg agatcacttt
     2641 gggagcttac cgtgttcata ccgtctgttt tcaacagttt tctccccggg aa
//
LOCUS       DAZOYL010000380         2674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZOYL010000380 DAZOYL010000000
VERSION     DAZOYL010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.384"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            27..611
                     /locus_tag="ACHUOY_003894"
     CDS             27..611
                     /locus_tag="ACHUOY_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0684524.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            716..1456
                     /locus_tag="ACHUOY_003895"
     CDS             716..1456
                     /locus_tag="ACHUOY_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0684525.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1491..2674
                     /gene="htrE"
                     /locus_tag="ACHUOY_003896"
                     /pseudo
     CDS             1491..2674
                     /gene="htrE"
                     /locus_tag="ACHUOY_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          761 a          564 c          581 g          768 t
ORIGIN      
        1 taatggaatt ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc
       61 ttatgctggc aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata
      121 tcagtggtct ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg
      181 gtctgatcct gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca
      241 ccgttggcgc taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga
      301 atccaggtag tacagctaaa atgaccttcg gttctgtttt ctttggtaat agcaaaggaa
      361 cgctgaacaa cgacatgtct attaacaacc cgtctgacgg cgtgaatatc gctctgcaca
      421 atattgatgg ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta
      481 aagccctgga tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg
      541 ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta
      601 cttatcagta agtattcacc aattaaattg attgaatgaa tatacaggga atagtgttaa
      661 taatttctat ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt
      721 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
      781 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
      841 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
      901 tactggcgat aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
      961 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
     1021 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
     1081 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
     1141 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
     1201 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
     1261 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
     1321 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
     1381 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
     1441 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
     1501 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
     1561 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
     1621 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
     1681 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
     1741 ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt
     1801 tcatataaat tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac
     1861 actcggcaat tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt
     1921 taacgagcaa cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa
     1981 ctatgttgat ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct
     2041 caacggatat catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa
     2101 cggtgggatg aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac
     2161 cgattctggc agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct
     2221 gcgttctcaa ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag
     2281 tatccgcggc attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt
     2341 tgcgcctatc attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg
     2401 ctataagatt tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc
     2461 gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac
     2521 attctcgcaa cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga
     2581 tattagcggc ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc
     2641 cagctactac tacggtctga ataactatct aacg
//
LOCUS       DAZOYL010000381         2644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZOYL010000381 DAZOYL010000000
VERSION     DAZOYL010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.385"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            12..107
                     /locus_tag="ACHUOY_003897"
                     /pseudo
     CDS             12..107
                     /locus_tag="ACHUOY_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            403..1398
                     /gene="pgl"
                     /locus_tag="ACHUOY_003898"
     CDS             403..1398
                     /gene="pgl"
                     /locus_tag="ACHUOY_003898"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0684526.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1439..2392)
                     /locus_tag="ACHUOY_003899"
     CDS             complement(1439..2392)
                     /locus_tag="ACHUOY_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0684527.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          673 a          588 c          648 g          735 t
ORIGIN      
        1 ggggatttac catgcttatt ggctatgtac gcgtatcaac aaatgaccag aataccgatc
       61 tacaacgtaa tgcgtttatt tgtgccggaa gtgagttgat tgttgagcga atgggaagtt
      121 ggccagaagt tgttatggca tagtgtttac ggtcgttgat acaggtaaac aaaagcgggg
      181 agagtcatac tccctgaata gtcgcgaaaa tatctgtggc attgtcaaat tcgtgtcggt
      241 gactaatatt ttagtaatat gccattcgaa atggagtgct aacttattga tttaattgat
      301 gtgcgatatt gtattttatc gtgttttgtt gtctgttttt tatccagtaa gtgttgttat
      361 gtgtatgaaa gataagggat ttatcatttt tgaggtgtgt tcatgaagca aacagtttat
      421 atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga aggcgcactg
      481 acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt cagcccggac
      541 aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta tcgtattgcc
      601 ccggacgatg gcgcactgac ctttgccgca gagtctgcgc tgccgggtag tccgacgcat
      661 atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg
      721 agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg
      781 gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt tccggcatta
      841 aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt ggcgcaggac
      901 cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac
      961 gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg ggaactgaaa
     1021 gatccacacg gcaatattga atgtgtccag acgctggata tgatgccgga aaacttctcc
     1081 gacacctgtt gggcggctga tattcatatc accccggatg gtcgtcattt atacgcctgc
     1141 gaccgtaccg ccagtctgat taccgttttc agcgtttcgg aagatggcag cgtgttgagt
     1201 aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga tcacagcggc
     1261 aagtatctga ttgtcgccgg gcaaaaatct caccacatct cggtatacga aattgttggc
     1321 gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc aatgtgggtg
     1381 gtggttaacg cacactaacc gctgatttac ccggcgcagt ctctcctgcg ccggtgtatt
     1441 aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac aaattgtctt
     1501 tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttctgctgcc ggagaaagca
     1561 cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc accagtggaa
     1621 taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc gccgaaatcc
     1681 ccagtcccgc ctctaccagt cctaatgacg tcgaaagatg acgcacttcg taaaaccagt
     1741 ccagcttcca gggcttgtcg gccagctgtt gttctatcaa cagtcggttg ccgctggagg
     1801 agcgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc tgctttttgg
     1861 ccaatggatg gtcacgccga caggccagca cgaacggctc gttgaccagt ggggtaaaat
     1921 caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg ttacacagca
     1981 ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata ttgggataca
     2041 gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc ggaatacacg
     2101 ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa agcgtttcat
     2161 cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct tttgttaacg
     2221 taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc atctttttta
     2281 ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg ttaaacgaac
     2341 tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc attattactc
     2401 cggaaaatgg aagcgacgat tttgggtggc tggccgttaa aaattttaac tgcatttagc
     2461 caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta gagatgcttt
     2521 gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca ttattatgaa
     2581 aaaaataccc tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc
     2641 gatg
//
LOCUS       DAZOYL010000382         2626 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZOYL010000382 DAZOYL010000000
VERSION     DAZOYL010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.386"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..472)
                     /locus_tag="ACHUOY_003900"
     CDS             complement(<1..472)
                     /locus_tag="ACHUOY_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0684528.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(472..1392)
                     /locus_tag="ACHUOY_003901"
     CDS             complement(472..1392)
                     /locus_tag="ACHUOY_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0684529.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1530..2429
                     /gene="pgrR"
                     /locus_tag="ACHUOY_003902"
     CDS             1530..2429
                     /gene="pgrR"
                     /locus_tag="ACHUOY_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0684530.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          665 a          609 c          593 g          759 t
ORIGIN      
        1 cgccaatcgg atcgtaattg ctgaaacctt cccgaaaaag acgcccgata ttaacacccg
       61 taatactcac tactgcttta attcgttttt ctgtcagcgc ggcattaatg gcatagccac
      121 cacccccaca cactcccagc acgccaatac gttcagcatt aacatatggc agagttactg
      181 cataatcgat aacacggcta atatcctcta cgcgctgtgt cggatcttct atccagcgag
      241 gctccccacc agattctccc tggaaactgg cgtcataagc gagaacgaga tagccttttt
      301 cagccaatgc cttgccatag atattgccag acgtctgctc cttacaactg ccaaaaggat
      361 ggacgctgat aatcgccgga tactgttttg actcatcaaa atttggtgga aactgaatat
      421 tggcagcaat atgccaggcc atatcactat tgcggatttg aatatgtttc attacagagt
      481 ttcctcaaag aacggagcta atactgaaat tgcttcagcc acataggctt tgccgtcata
      541 aagatccata tggtttgcgc cttcaacaat gtgatagcgt ttatcttggc ttgatgctcg
      601 atcgtacagg tcgtcactca tccatttgct ccctgcctgg ctgcccgcca caatctgcat
      661 cggctgagtc aggtacactt ccgccatatg gtaagcatca aaggtaataa tctggttaag
      721 gctgcgcaaa gtagcgtaac ccggtgctgt tggatactgc gcgcgagggg tgtggtaata
      781 ctcccaggcc tgacgcagtt cttcgttcgg cgcatcggac tccttcattg gtgccagtgg
      841 cataacggca tattctccgc tgctaatatc gctggttcgg gcgtttgaac ccgcttcaac
      901 gtatggcagg gcatcaatag atttcacatt gttttcccaa ccattacgga acatcgaacc
      961 aatattgacc gcactaacgg taccgatggc cttgatgcgg cgatcctgaa ttgcagcatt
     1021 ggctgtatat cctgcaccgg cacaaattcc catcgcacca attcgggtat tgtcgacata
     1081 agaaagcgtt gtcaggtaat caatcacggc actgatgtct tcagtacgaa tgtatgggtt
     1141 ttctaactga cgcggctcgc cgccactttc accttgataa gatgcgtcat aagcaatagt
     1201 gacaaatccc ttttccgcca gttttttggc ataggttccg gccgtttgtt ctttaacgcc
     1261 cccacctggg tgagatacca caattgcctg atactgacgg gtttcatcaa attttggagg
     1321 gaaatagatc actgcagaca aagagatggt tggattattg ctgttagtga agctgacttt
     1381 attattcatc attccgttcc tcatggagtt gtcggttcgt tttaacggtt ggtgatatca
     1441 ctatagatat tgaacattaa gttgattaga cccaaaatta tgattagacc tatcatttaa
     1501 atgattaata gattgcatgg agatactgaa tgaagcgtga agaaatcgct gatctgatgg
     1561 cgtttgtcgt cgttgcagag gagcgtagct tcactcgtgc agcagcccgc ctgagcatgg
     1621 cgcagtcagc tttaagccag atagtgcgtc gtatagaaga acgattggga ttgcggcttc
     1681 tgacgcgaac cacgcgcagc gttgttccaa ctgaagcggg cgagcatctt ttgtctgttc
     1741 ttggccctat gttgcatgac atagattcag ccctggcatc cctgagcgag ctgcagaacc
     1801 gcccatccgg gacaatacgt attactactg tagaacatgc agccaaaacg atattgttac
     1861 cagcaatgcg cacattcctg aaatcgcatc ctgaaattga tattcagctc accattgatt
     1921 atggtttgac cgatgtcgtt tctgaacgtt ttgatgcagg cgtccgtctg ggtggggaga
     1981 tggataaaga tatgatcgcc attcgaatcg ggccagatat accaatggct attgttggct
     2041 caccggatta tttttctcgc cgaagtgttc caacgtcagt gtcacaatta atagatcatc
     2101 aggcaattaa tttgtatctt cccacatcgg gtacagcaaa tcgctggaga ttaatacgcg
     2161 gtggacgtga agttcgtgtt cgcatggaag gtcagctttt actgaatacg atagacctga
     2221 tcattgatgc tgcaattgat gggcatggat tggcgtatct accttatgat caggttgagc
     2281 gggctattaa agaaaaaaaa ctgatacgtg ttttggataa attcacacca gatttacccg
     2341 gttatcacct gtactatcca caccgtcgac atgctggctc ggcattctca ttatttatag
     2401 ataggctgaa gtataaaggt gctgtttagc actacttgct gatacattaa tttaattttt
     2461 ctcttaacgt attctcagtt cctttcaacg ttttggttat tttttattct tcgtacaatg
     2521 gcgacagatg ctgattatga taccgaaaac tggtttgaac gtgcgaagcc tgaacgtagt
     2581 gttcggtgtt ctatgtgctt taccgcattt tgtcgtctat tattta
//
LOCUS       DAZOYL010000383         2621 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZOYL010000383 DAZOYL010000000
VERSION     DAZOYL010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.387"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            51..560
                     /gene="eco"
                     /locus_tag="ACHUOY_003903"
     CDS             51..560
                     /gene="eco"
                     /locus_tag="ACHUOY_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR0684531.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(869..2515)
                     /gene="mqo"
                     /locus_tag="ACHUOY_003904"
     CDS             complement(869..2515)
                     /gene="mqo"
                     /locus_tag="ACHUOY_003904"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR0684532.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          621 a          728 c          652 g          620 t
ORIGIN      
        1 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
       61 aaagcaaaaa aatgaagacc attctacctg cagtattgtt tgccgctttc gccaccactt
      121 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
      181 aaaaagggat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
      241 aagtagaact gttaaccggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
      301 ggaagctgga aaacaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
      361 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
      421 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
      481 tgtatacgcc agacaatgta gatgtgaagt accgcgtctg gaaggcggaa gagaaaattg
      541 acaacgcggt agttcgctaa actgctgtga agtgcgtcac cccgtaggtc ggataaggcg
      601 gtcacgccgc atccgacatc taacgcccaa gccggttgcc tgatgcgacg ctggcgcgtc
      661 ttatcaggcc tacaccgctg tgaagtgctc caccccgtag gttggataag gcggttacgc
      721 cgcatccgac atctaacgcc cgagccggtt gcctgatgcg acgctgacgc gtcttatcag
      781 gcctacaccg ctgtgaagtg ctccaccccg taggtcggat aaggcggtta cgccacatcc
      841 ggcataccat gccggatgtg gcacatcatt acaacgcaat atccgccact tctttttgaa
      901 cgggttgcgg tttcaactgc ggtttcggcg tactatctgc tgcctgcggc ttgtcgtatt
      961 tcaggcccag cacttcgctg gtgtactgca actcgcgttc tgttgccgct acatcaccgt
     1021 tcagcttgcg tccataagat ggaacgatcg ctttcaacgt agcctgccat tgcgggctgg
     1081 aaacgcgatc gccaaatact ttttccagca gatccaacat aatcggcgca gcggttgacg
     1141 cccctggcga tgcccccagc agcgcggcaa tggtcccttg ctggtcactg accacttcag
     1201 tacccagacg cagtacacca cctttatcgg catcacgctt gataatctgc acacgctgcc
     1261 ccgcttgcca caaacgccag tcctcttttt tcgcctgcgg atagtactct ttcaacgctt
     1321 caaaacgatc ctcttcactc aacatcacct gactcaccaa gtatttcacc agatcgaaat
     1381 tatccagccc gacgtgcatc atcggcatca cgttagaggt ggtggtggaa ctcatcagat
     1441 cccacaatga accgtttttg aggaatttgg ttgagaaggt ggcaaatggc ccaaacagca
     1501 ctacgcgttt accgtccaga acgcgggtat cgatatgcgg aaccgacatc ggtggtgcgc
     1561 caacggatgc tttaccgtaa accttcgcca gatggtgatt aaccacgtcc gggttttccg
     1621 aaacaaggaa ctgtccgccc accgggaaac cggcgtagtc tttcgcttcc ggaatccccg
     1681 attcctgtaa cagcttcagc gccgcaccgc ccgcgccgat aaagacaaat ttggcacgaa
     1741 tgttctgtgc agtgccattt ttcagatcgg caacggtaac ggtccaggtg ttatcgtcat
     1801 tacgctttag ggcgcggact tcgctgctga gttgcagcga gaagttagat ttcttctgca
     1861 aggaagcaat taactggcgg gtgatctcgc cgtagttcac atcggtacca atttccgtac
     1921 gcgtggctgc cactttctgt tgcggatcgc gcccttccat cactaacggt gcccactctt
     1981 tgatctgcgc gtgatcttca gagtaacgca taccgcgaaa cagcgagctt tgttgcaacg
     2041 cggcgtaacg ggcgcgcagg aaattaacgt tatcctcgcc ccagacaaag ctcatatgcg
     2101 gaacggtatt gataaatgaa cgcggagtac gcagcacgcc gcgctcgacc tggtgcgccc
     2161 agaactggcg ggaaatctga aatgcttcgt taatggcgac tgctttttca atactgatac
     2221 tgccatcggc gttttgcggg gtgtagttca gttccatcag tgcagaatgc ccggttccgg
     2281 cgttgttcca gccgttcgaa ctctcctgcg cgacaccctc caggcgctcc accatggtca
     2341 tcgaccattc aggctccagc tcgcgtaaat aggtccccaa cgtggcgctc ataatgccgc
     2401 cgccaatcaa cagtacatca gtttcctgct cctcggacgc tttcgctttt gccgccatcg
     2461 aaacggcatt aagccccacg gccatcgaga agagcatggc agtcactttt ttcatcttgt
     2521 taatgcctta cttttagtcg ctttattgca ggtgagattt gcgcggcatc aacggtaaca
     2581 ctaaagtaac aacatttaaa taatgtttat aaattatatt c
//
LOCUS       DAZOYL010000384         2615 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZOYL010000384 DAZOYL010000000
VERSION     DAZOYL010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.388"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            88..279
                     /gene="hemP"
                     /locus_tag="ACHUOY_003905"
     CDS             88..279
                     /gene="hemP"
                     /locus_tag="ACHUOY_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR0684533.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(283..1719)
                     /gene="selO"
                     /locus_tag="ACHUOY_003906"
     CDS             complement(283..1719)
                     /gene="selO"
                     /locus_tag="ACHUOY_003906"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR0684534.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1782..2495)
                     /gene="ydiV"
                     /locus_tag="ACHUOY_003907"
     CDS             complement(1782..2495)
                     /gene="ydiV"
                     /locus_tag="ACHUOY_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR0684535.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT          716 a          673 c          586 g          640 t
ORIGIN      
        1 ttgtcttctc aacaaattac tgcttgctct ggcctgccat aatattgata ataagaatca
       61 ttgttatatc aattattatt aatctttatg cgttatacgg atagcagaaa actcacgcct
      121 gaaacggacg ccaatcacaa ggccgcttcc ccgcagccca tccggcgaat taccagccag
      181 acactgttag gtccggatgg caaactgatt atcgatcatg acgggcaaga atatctgctc
      241 cgtaaaactc aggctggcaa gctgctgtta actaagtagc ctttaactcg agcagctgac
      301 ttccagccgt ttaccccagt caggtggacg actgacatag tcatcatctc tatcgctgaa
      361 aggatttcgc aacgccccat gcaggcggtg caattccgtc atatcaccct tttctgccgc
      421 ttcaatcgcc cgttgcgcca accagttgcg caataccaga gcggggttaa cgctttgcat
      481 caactgctga cgctcactat cgctaacctc gtcttgttgc aaacgccccc gataacgggc
      541 aaaccagcca tcaaatgccg cacgatcaat aaactcatca cgtagcggtg acgccgtgct
      601 gtgctgctcg gtcagactca gcatgcggaa tgtgcgggta taatcgctgc gctctcgcgc
      661 catcagactg aataattcat tcagtagcgc gttatcctct ttttgctccg tgatgaagcc
      721 cagtttctgc cgcatccgtt gtccataatg cgtcaacaaa acctgctgat agctgtccag
      781 ggcctcattc agggcatcta cggcaacaaa tggtgacaat gtctgcgcca gacgctgtaa
      841 attccacaac gcgacagcag gttgattatc aaaactataa cgcccttgat gatccgagtg
      901 attacaaata aaaccgggtt cgtaatcatc aagaaaacca aacggcccgt aatcaagcgt
      961 cagtcccagc agcgacatgt tgtcggtatt catcacccca tgtgcaaagc cgaccgtctg
     1021 ccattgggca attaacgacg cggtacgtgc gacaacatcg ctaaaccaga gacggtattt
     1081 gtcctcatca tcttcaagat gtgaccagta atgacgaata gcaaagtcag ctaactgacg
     1141 aaccttttcc gactcgcggc ggtagtaaaa atgttcgaaa tgaccaaagc gcagatgact
     1201 tggtgccaca cgcatcagca tcgcgcctgg ctccgccgtt tcccgataca ctggcgaatc
     1261 gctggtgacg atacttaacg cgcgggtcgt cggaatgccc agataatgca tcgcctcact
     1321 ggcgagactt tctcgtatcg tcgaacgtaa aaccgcccgt ccatcaccca ttcgcgaata
     1381 aggcgtcagg ccagcacctt tcagatgcca gtccattgta gtgccatcag caagcagttg
     1441 ttcgccgagt aaaatgccgc gcccatcacc cagttggccc gcccagacgc cgaactgatg
     1501 accactgtaa acctgcgcca gtggtgacat accaggcagt agggcttcgc cgccccagac
     1561 acctgcgcca tttttaaaca gcgacgatgg aatactcagc gtgttagcca gttcggtatt
     1621 atgccaaatc agccgggcat tatttaaagg cgtaggggaa agtgctgtat aggtttctgg
     1681 caattcatcg cgccagcggg taacaaaaga cagggtcata gatcctcctg tttgatagtg
     1741 tagacggtta ctctcgtctt aaacaccagc aaacagaagg gttatcgctg aaccaacgtc
     1801 gttatctgat ttatgggaac ggcaggccac aggcatccct ggagagcatg gaaatcgaag
     1861 ggggtaatct gtgccaatat ttctgccgta tcaatgcctc ccgcaatgat gcaattgcaa
     1921 caaggggaaa tttgcgcctg gatcgcacga ataaacggtt cgaaggagcg gtgcgtgatt
     1981 tgttgctgaa taaaactttt atccagcatg acgcgggtga aaagaccatc aaaaaccgct
     2041 ttcattgtgc tattgcctgc tcccaaatta cctaacacaa ggggatacac ttgcgataag
     2101 gataaaagac cgcgattatc ttttccttca ttgagatgcg ggtaattctc attaatcaac
     2161 agttcaataa acggatattt taatagttcg cctgcgtaat tatccctctc taataataat
     2221 gtagccactt gtggcgttaa attaagccag gcaaagagtt tatgttgaat aaaaaaatgt
     2281 tggcatgatt ttagtaattc caactgctct gaaaataatt gccaatgttg ctcctcggta
     2341 agttgcgcta taacccgact cgtcgggata cgcaccgtgc catcttcact ggagaaatgg
     2401 gtaattaact caacgccgac aaggacttgc tggttatctc ttatcggcag gaaatagcaa
     2461 tcagaatgat aaagattctc caaaaaaatc ttcatggtaa ccgtccctct cgaaggatgt
     2521 ttttcagtat ccggctaaaa gggatgaggc cataagacat aacaattaca gaaggagtaa
     2581 ctttcatttg ttccatgtta accacttttt caggg
//
LOCUS       DAZOYL010000385         2610 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZOYL010000385 DAZOYL010000000
VERSION     DAZOYL010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.389"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            111..443
                     /locus_tag="ACHUOY_003908"
     CDS             111..443
                     /locus_tag="ACHUOY_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR0684536.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="ACHUOY_003909"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="ACHUOY_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR0684537.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /gene="yegR"
                     /locus_tag="ACHUOY_003910"
     CDS             complement(2281..2598)
                     /gene="yegR"
                     /locus_tag="ACHUOY_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR0684538.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT          682 a          680 c          619 g          629 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcccc
//
LOCUS       DAZOYL010000386         2598 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZOYL010000386 DAZOYL010000000
VERSION     DAZOYL010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.390"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(112..864)
                     /gene="tcyN"
                     /locus_tag="ACHUOY_003911"
     CDS             complement(112..864)
                     /gene="tcyN"
                     /locus_tag="ACHUOY_003911"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR0684539.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(861..1529)
                     /gene="tcyL"
                     /locus_tag="ACHUOY_003912"
     CDS             complement(861..1529)
                     /gene="tcyL"
                     /locus_tag="ACHUOY_003912"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR0684540.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1544..2530)
                     /gene="dcyD"
                     /locus_tag="ACHUOY_003913"
     CDS             complement(1544..2530)
                     /gene="dcyD"
                     /locus_tag="ACHUOY_003913"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR0684541.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT          619 a          730 c          646 g          603 t
ORIGIN      
        1 tcgcattctc ttatgttatt aatgagttat gctgatttgt taagcagttt tatcacgctt
       61 gaaatggcgt cccgccccga caggtgaatc gtcggggctg attttttctt attattgcag
      121 tagaaacttc tcgaggaact ggcgggtgcg aggctgctgg ggatcagcaa ataacgcttt
      181 tgctgccccc tgctcgacta tccgcccctg gtccataaaa atcgcccggt ccgcaacatc
      241 ccgggcaaag ctcatttcgt gcgtcacaat caccatcgtg cgcttttcct gcgccagctg
      301 gcggattgtg ttcaggactt cgcccaccag ctctggatcc agcgctgacg ttggctcgtc
      361 aaacaaaatc acctcaggac gcattgccag cgcacgcgca atcgctacac gctgctgttg
      421 accgccagac aaacgacgtg gatagctggt ttctttaccg gccagcccaa ctttcgccag
      481 cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg cctttgacga tcaccggccc
      541 ttcaataatg ttttccagca ccgtacgatg cggaaacaaa ttaaagttct ggaagacaaa
      601 cccgacgtgc tgacgcaact ggcggatcag cgatttttgc tgacttaatg aacgtgcagt
      661 atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc ggttgttcca gcagatttat
      721 gctgcgtagc aacgtggttt tgccggaacc actcggaccg ataattgcca ccacttcgcc
      781 aggctttacc tcgaggtcga taccgtgcag caccgtctga ccgtgaaatt ttttcaccag
      841 gttcttaact tcaatggcac tcattttggt tctctctcct ggcgattaag ttgattctca
      901 aaatggttct gcaacgtcga taatactgtc gccatgatcc agtagatgag cgaagccgcc
      961 aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca actgcgcctg acggaacagc
     1021 tccggcacct ggattgtcgc ggccagcgac gtatccttta ccaggctgat aaagctgttc
     1081 gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa tcgcgcgacg catagtctgc
     1141 catggcgtca taccaatact ggcagctgct tcccactgac ctttatcaat agaagaaatg
     1201 gcggcgcgca gtgtttcggc ggcataggcg gcagtgttca gcgacagacc aatcattgct
     1261 gacggaatgg gatctaattc gataccaaac tgcggcaggc cgtaatagat cataaacagt
     1321 tgtgcgatga gcggtgtacc acgaaaaata gagatataaa aacgcgccag ccagcgcacg
     1381 ggccagattg gcgacaggcg catcagcgcg agaataaacc cgagcagtaa gccaaaaaac
     1441 atgccgccga tactgagttg cagcgtatac ccagcccctt tcagcaggaa cggcaaggaa
     1501 tcaataacca gttgtatact ttcttgcatg agcgttcccg gagctaaacg tggggatgat
     1561 aggcgaacag cgcaggcgca ccgccggtat gaataaacag aatcggccct tcatctttga
     1621 agcgtttctg actgatacag ccaatcagcc ccgccatcgc ttttccggta tacacaggat
     1681 caagcagaat gccttccagc cgcgccagca atttcactgc ttccatgcct tcgtcgttcg
     1741 gcacgccgta gccaggtgca aaatagtcat cccagagtat aatttccgct gatgcggtca
     1801 gctccagttc tttcgcaatc gcctgttgta ggttaaccac tttcggcaac tgatcggcaa
     1861 cggaacgcga cacggtcacg ccaatcagtt cgctttcagg catcaggtgt tccagcccaa
     1921 cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac taccaccgac gaaatattaa
     1981 ccgccccttc acactgttgc gcgatttcca gcgcactctc cacataacct agcgcgccaa
     2041 gagcattaga accgccaacc ggaatgacat acgggcgaaa gccttgtgct tcgactcgcg
     2101 tcgccagctc ttccagttgg gcattgggat cggtcagtgc ttcgcacatt tcaatctggg
     2161 tattgaacag atccagcaac aagcgattgc cgttggttaa atagttttct gcggttgtgc
     2221 caataggatt ttccagcagc gccacacagt gcagaccgag tttcgccgca actgcggcag
     2281 tctgccgcac atggttagac tggatcaccc cggcagtaat cagcgtatcg gcaccttcgc
     2341 gcagagcatc tgccgcgaga aattctagct tacgtaattt attgccgccc attgccatgg
     2401 gggtgacgtc atcccgtttg atgaaaattt cccgtcctag ataatcagaa aagcgcggca
     2461 gatattcgag cggcgttggc gcgccgataa actccaaccg tggaaaacgg gttaaattat
     2521 gcagtggcat aacagcctcc gatgtatgtt gttgtgattt tcttattatg cacgctgaaa
     2581 acgcgtaaat aaaaaagg
//
LOCUS       DAZOYL010000387         2598 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZOYL010000387 DAZOYL010000000
VERSION     DAZOYL010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.391"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            82..1695
                     /gene="mppA"
                     /locus_tag="ACHUOY_003914"
     CDS             82..1695
                     /gene="mppA"
                     /locus_tag="ACHUOY_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR0684542.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1746..>2598)
                     /gene="ynaI"
                     /locus_tag="ACHUOY_003915"
     CDS             complement(1746..>2598)
                     /gene="ynaI"
                     /locus_tag="ACHUOY_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR0684543.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT          746 a          584 c          628 g          640 t
ORIGIN      
        1 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
       61 atgtcggtta gagggaaact tatgaagcac tctgtttcag tcacgtgttg tgcgctgttg
      121 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      181 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      241 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggactggt gaatcagaac
      301 gagaaagggg agattgtccc cggcgttgcg actcagtgga aaagtaatga caaccgtatc
      361 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
      421 gattttgtct acagctggca gcgtctggtg gacccgaaaa cgctatcgcc atttgcatgg
      481 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcca
      541 gaccagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
      601 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
      661 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
      721 cttaaagatc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
      781 aacgccaaaa cggtactgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
      841 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
      901 cagaagctgt tgaaggatat tccggggcag gtttatacgc cgccgcagct cgggacctat
      961 tattatgcgt ttaacacgca aaaagggccg acggcagatc agcgcgttcg tctggcatta
     1021 agtatgacga tagatcgccg cctgatgacc gaaaaagtat tagggacggg cgaaaagcca
     1081 gcgtggcatt ttacaccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1141 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1201 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1261 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     1321 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     1381 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaacg
     1441 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     1501 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     1561 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaagccg
     1621 tggctgaaag gttatcccat taataacccg gaagatgtgg cgtacagccg gactatgtat
     1681 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     1741 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     1801 aaggcaaagt ccgcgccgtg tgactgcaca atatcgataa tcttcaaata aacgtcttgc
     1861 tgtgcagcaa gccattcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     1921 aatgacgagt cggcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     1981 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2041 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2101 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2161 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2221 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     2281 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     2341 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag caccacaata
     2401 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     2461 atctgctgtt tttcaaactg attaatcagc aaaaatagct taatcgtcag aataaccgca
     2521 atcagggacg tacagataaa atcgataacg tctggggtga taaatttgag tttatagttt
     2581 tctataacat aattaata
//
LOCUS       DAZOYL010000388         2567 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZOYL010000388 DAZOYL010000000
VERSION     DAZOYL010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.392"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1311
                     /gene="gdhA"
                     /locus_tag="ACHUOY_003916"
     CDS             <1..1311
                     /gene="gdhA"
                     /locus_tag="ACHUOY_003916"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR0684544.1"
                     /translation="NHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSLLERLV
                     EPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSILKFLGFEQ
                     TFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDVPAGDIGV
                     GGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMG
                     FEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKA
                     SRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIANGVKAVAE
                     GANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLH
                     HIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(1428..2467)
                     /locus_tag="ACHUOY_003917"
                     /pseudo
     CDS             complement(1428..2467)
                     /locus_tag="ACHUOY_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          671 a          615 c          610 g          671 t
ORIGIN      
        1 aaccatgtcc aaaagcgcga cccgaatcaa accgagttcg cgcaagccgt tcgtgaagta
       61 atgaccacac tctggccttt tcttgaacaa aatccaaaat atcgccagat gtcattactg
      121 gagcgtctgg ttgaaccgga gcgcgtgatc cagtttcgcg tggtatgggt tgatgatcgc
      181 aaccagatac aggtcaaccg tgcatggcgt gtgcagttca gctctgccat cggcccgtac
      241 aaaggcggta tgcgcttcca tccgtcagtt aacctttcca ttctcaaatt cctcggcttt
      301 gaacaaacct tcaaaaatgc cctgactacc ctgccgatgg gcggtggtaa aggcggcagc
      361 gatttcgatc cgaaaggaaa aagcgaaggt gaagtgatgc gtttttgcca ggcgctgatg
      421 accgagcttt atcgtcatct gggcgcggat accgacgttc cggcaggtga tatcggcgtt
      481 ggtggtcgtg aagtcggctt tatggcgggg atgatgaaaa agctctccaa caataccgcc
      541 tgcgtcttca ccggtaaggg cctttcattt ggcggtagtc ttattcgccc ggaagctacc
      601 ggctacggtc tggtttattt cacagaagca atgttaaaac gccacggtat gggttttgaa
      661 gggatgcgcg tttccgtttc tggctccggc aacgtcgccc agtacgctat cgaaaaagcg
      721 atggaatttg gtgctcgtgt gatcactgcg tcagactcca gcggcactgt agttgatgaa
      781 agcggattca cgaaagagaa actggcacgt cttatcgaaa tcaaagccag ccgcgatggt
      841 cgagtggcag attacgccaa agaatttggt ctggtctatc tcgaaggcca acagccgtgg
      901 tctgtaccgg ttgatatcgc cctgccttgc gccacccaga acgaactgga tgttgacgcc
      961 gcgcatcagc ttatcgccaa tggcgttaaa gccgtcgctg aaggggcaaa tatgccgacc
     1021 accatcgaag cgactgaact gttccagcag gcaggcgtac tgtttgcacc gggtaaagcg
     1081 gctaatgctg gcggtgtagc aacgtcgggc ctggaaatgg cacaaaacgc tgcgcgcctg
     1141 ggctggaaag ccgagaaagt tgacgcacgt ttgcatcaca tcatgctgga tatccaccat
     1201 gcctgtgttg agcatggtgg tgaaggtgag caaaccaact acgtgcaggg cgcgaacatt
     1261 gccggttttg tgaaggttgc cgatgcgatg ctggcgcagg gtgtgattta agttgtaaat
     1321 gcctgatggc gctacgctta tcaggcctac aaatgggcac aattcattgc agttacgctc
     1381 taatgtaggc cgggcaagcg cagcgccccc ggcaaaattt caggcgttta tgagtattta
     1441 acggatgatg ctccccaggg aacatttctt atgggccaac ggcatttctt actgtagtgc
     1501 tcccaaaatt gcttgtcgta acgataacac gcttcaagtt cagcatccgt taactttctg
     1561 cgatagcagc agatatgcca gtaaagaaac cccattagac tatttttttg ataatcttct
     1621 tcgctttcga acaactcgtg cgcctttcga gaagcaagca ttatataatg ccaggccagt
     1681 tcttcttcaa ttgtcccgtt ttgaaaagct gtgcttgata tcgaggtcat ccatgataat
     1741 tccgccgccc atattagctt cgccgaggat ttaccggagc gattattagc gcaatcagag
     1801 atatagtctg agggaaaaac agcaaattta ttcaacaagg cgataacctg ctctggggct
     1861 tcctccatgt ttgctttaaa ggtattggct ccatggtcgc cagaaagaaa atgctccatt
     1921 aggcacaata actttcgcta tcttcgatac cccattgatt ctctaaagac tcgcgtcttt
     1981 tactgatgat atcgatcgag tcaaaaggaa gcacatgata ttggaaggta tctttaccag
     2041 gttcaggctt tcgcggccag aactctagcg tttcagacca ttgcttatga tagaatcgat
     2101 aaggtgcgat caattgtagc gcctgtaact tctcgatact gagcggctca atacctttag
     2161 cctgataata atgcagttgt tctttttttg ctttaaaacc ggcccgaaca ataagcccca
     2221 tcataattaa tagataaaga aaagagcatc ccgcggtaat caggcctctt tcattcaaac
     2281 cgttggatgt tatcgctgcg aacacaaaca ttacagcgac aacacatgtt aaataaaacc
     2341 cccacttaca aagcagcatg gccttatttt ctttaatcat ccgttcaaaa ttactattaa
     2401 atatttccca gccattaaaa gaatacttct cgctcccagg atggttttgt aataaaactt
     2461 ttttcatcac attccctgtt attacatact ttagaggcga taattttatc catgcaaaaa
     2521 aaatatccaa cgaattttct tgatccgctg gatatttcag aatatga
//
LOCUS       DAZOYL010000389         2556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZOYL010000389 DAZOYL010000000
VERSION     DAZOYL010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.393"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUOY_003918"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUOY_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR0684545.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHUOY_003919"
     CDS             complement(390..1178)
                     /locus_tag="ACHUOY_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR0684546.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUOY_003920"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUOY_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR0684547.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUOY_003921"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUOY_003921"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0684548.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHUOY_003922"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHUOY_003922"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR0684549.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          665 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgt attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAZOYL010000390         2541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZOYL010000390 DAZOYL010000000
VERSION     DAZOYL010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.394"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1361)
                     /locus_tag="ACHUOY_003923"
     CDS             complement(<1..1361)
                     /locus_tag="ACHUOY_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0684550.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="ACHUOY_003924"
     CDS             complement(1426..1545)
                     /locus_tag="ACHUOY_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0684551.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1769..1927)
                     /locus_tag="ACHUOY_003925"
                     /pseudo
     CDS             complement(1769..1927)
                     /locus_tag="ACHUOY_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2272..>2541)
                     /locus_tag="ACHUOY_003926"
     CDS             complement(2272..>2541)
                     /locus_tag="ACHUOY_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684552.1"
                     /translation="FDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKII
                     TGVAPFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT          679 a          607 c          483 g          772 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cgcctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgattttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa a
//
LOCUS       DAZOYL010000391         2540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZOYL010000391 DAZOYL010000000
VERSION     DAZOYL010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.395"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(14..88)
                     /locus_tag="ACHUOY_003927"
     tRNA            complement(14..88)
                     /locus_tag="ACHUOY_003927"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Gln,seq:ttg)
     gene            complement(112..196)
                     /locus_tag="ACHUOY_003928"
     tRNA            complement(112..196)
                     /locus_tag="ACHUOY_003928"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Leu,seq:tag)
     gene            complement(205..281)
                     /locus_tag="ACHUOY_003929"
     tRNA            complement(205..281)
                     /locus_tag="ACHUOY_003929"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:Met,seq:cat)
     gene            complement(661..2325)
                     /gene="asnB"
                     /locus_tag="ACHUOY_003930"
     CDS             complement(661..2325)
                     /gene="asnB"
                     /locus_tag="ACHUOY_003930"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR0684553.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(2279..>2540)
                     /locus_tag="ACHUOY_003931"
     CDS             complement(2279..>2540)
                     /locus_tag="ACHUOY_003931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684554.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPDPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT          610 a          639 c          628 g          663 t
ORIGIN      
        1 attgtttctt cgatggctgg ggtacctgga ttcgaaccag ggaatgccgg tatcaaaaac
       61 cggtgcctta ccgcttggcg ataccccatc cgtacaacgc tttctggtga atggtgcggg
      121 aggcgagact tgaactcgca caccttgcgg cgccagaacc taaatctggt gcgtctacca
      181 atttcgccac tcccgcaaaa aagatggtgg ctacgacggg attcgaacct gtgaccccat
      241 cattatgagt gatgtgctct aaccaactga gctacgtagc catctttttt tcgcgatacc
      301 ttatcggcgt tgcggggcgc attatgcgta tagagccttg cagcgtcaac ctctttttca
      361 aggaaaattg ctcgaaagtg actgtttggt taggttgcga acagcgtggc gctatattcg
      421 tcaattattg tttactttgt gttttttccc caccctacag ccattctttt gtcatacagg
      481 atgaaattcg gaattcaaca atggtggtgg gaaattaatc tatgaaatac tggcctacag
      541 tgatgagttg tcaaacagtg atgtggcaaa cccggaacat ttccttactg catatcagaa
      601 tcaacaagca cctcaataac tgaaacagcc ccggatttca ccggggctgt ttcgcatttc
      661 ttacttatac gccgactggt gaacgccaac cgcacgacca gacggatcgt ccattttctt
      721 gaacgcctca tcccactcaa tcgctttagc ggaagaacac gcgacggacg ggccgcccgg
      781 cacgcactca gcggcgctcg gaagcgggaa cagttcttca aagatctccc ggtacagata
      841 cgcttctttt gaggttggcg tgttgtacgg gaagcggaag cgggcagttt ccagttgctg
      901 atcagaaacc tgctgcgccg ccacttcttt cagggtgtcg atccaactgt aaccgacgcc
      961 atcggagaac tgctctttct gccgccaggc cacgcttgcg ggcaggtatg actcaaaaca
     1021 ttcacgcagg atgtgttttt ccattttgcc gttaccgcac attttatcct gcgggttaat
     1081 gcgcatcgcc acatcaagga attttttgtc gaggaacgga acgcgtgctt ccacgcccca
     1141 ggctgacatc gctttgttgg cgcgcgcgca gtcatacata tgcagggcca gcagtttacg
     1201 caccgtctct tcatgcagtt ctttagcgtt gggcgcttta tggaagtaaa gataaccgcc
     1261 aaacacttca tcagaacctt caccggacag caccatttta atgcccatcg ccttgatctt
     1321 acgcgacatt aaatacatcg gtgttgaagc gcgaatagtg gtcacatcat aagtttcgat
     1381 gtggtaaatc acgtcgcgga tggcatccag accttcctgt acagtgaagt gaatttcgtg
     1441 atgcaccgtg cccagatggt ttgccacttc ctgggctgct ttaagatccg gtgaacccgg
     1501 cagacctaca gcaaaggagt gtaactgggg ccaccaggct tcagagcgtt cctgatcttc
     1561 cacgcgacgg gctgcgtatt tcttggtgat agcggaaata attgaggaat ccagaccacc
     1621 agaaagcagc acaccgtaag gcacatcaga catcagatgg cttttaacgg aatcttccag
     1681 agcctgacgc agctcgtttt tgtcggtcac gttatctttc actgcatcaa agtcgaacca
     1741 gtcgcgatga taataagaac ggatttcgcc gtcctggctc cacaaatagc tccccgccgg
     1801 gaactcttta atcgtgcggc aaactggcac cagcgatttc atttctgagg ccacatacag
     1861 ctgaccgtgt tcgtcatacc ccatatacag tgggatgatc cccagatggt cgcgaccaat
     1921 caggtaggca tctttttcgc tgtcgtacag ggcaaaggca aacatgccct gcaagtcgtc
     1981 gagaaattcc ggcccttttt cctgatacag cgcgaggatc acttcacagt cagaccctgt
     2041 ctggaactga taacgatcgc catattcagc gcgcaatgcc tggtggttgt agatttcacc
     2101 gtttaccgcc agtacgtggg ttttttgttg gttgtagaga ggttgcgccc ccgcgttaac
     2161 gtcaacaatt gacagacgtt cgtgagcgag aatggcgtta tcgctggcat aaataccgga
     2221 ccagtccggg ccacgatgac gcatcaggcg tgacagctcg agtgctttct tacgctgctc
     2281 aactgcgtct gttttgatat cgaatacgcc aaaaattgaa cacataacct tctccgttaa
     2341 cctggtattt gttgcttgtt gtgtttgctt gtttaaaaaa atgccgcaaa gcagcactgt
     2401 gcgcaagcga tttggcggtg aaaaaataaa aaacgtaatg gtgattgtcg attggtgaaa
     2461 aagggtctgg tgtgaggatg tatttattga tgaatcgata atttttagcg ggttttattg
     2521 aatggttata ttttacgggg
//
LOCUS       DAZOYL010000392         2531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZOYL010000392 DAZOYL010000000
VERSION     DAZOYL010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.396"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1187)
                     /locus_tag="ACHUOY_003932"
     CDS             complement(<1..1187)
                     /locus_tag="ACHUOY_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0684555.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLG"
     gene            1836..2351
                     /locus_tag="ACHUOY_003933"
     CDS             1836..2351
                     /locus_tag="ACHUOY_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0684556.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT          765 a          538 c          435 g          793 t
ORIGIN      
        1 tcacctaaac tgccgttttc aaaacaacat tttttcaata tgctttcatt cacaacagtg
       61 tgtgataagt agagattact tatgttttgc tttattaatg ccacgtcatt aaaatgacta
      121 tatttaatga aaaaaccatc attgagtagt gttttaagat tgactaaatt acttgcatct
      181 tcacgtacca tcgtggcaac tggaacaggc agagacatga aaacttcttc attcaccatc
      241 aagtgaatca tgcattgcgt tccctttgtc atatccacac atacgctaca aatatcgtgg
      301 taatgcgaag agcgcatttc aggaaacaga ttaaacacgg tatcttgaaa ggcactaaat
      361 cgtgttaata aatcctgata atgaacaggt tttgtttcaa aattatcatc agcaaatcca
      421 agcactattg tatgctctac atctaacgca gtttcaaatg gctcttcttc tgtaaaatca
      481 ttatttaaaa cctcacctga cgtattatca ctgtcataaa aagtgttcat caatgcgata
      541 ttgtttgtta cagcgtcttt ttcgatattc tcactaattg catatcgcct gctataatca
      601 taaaaatgaa taaacgcgcc ctttataaag gatatatatt gttctttgtt aaagtcaata
      661 cggctgtata atttttcaaa aatcacattc gcaattttac taccaatggt atgcctgtat
      721 aataatgggt gcgattcaat cgtaaaatgc ttgtctaatt gatcttgtaa tttattctca
      781 tagtccagag ggaaaagaaa ctgttgtctt tcaagtttcc cattcgatac attacgtatt
      841 ccaatagaga ttctttcatt acccgccttt atttcaactt cgttatcttc tgatttaccc
      901 agatattttt taacatcagt ctcgaaagca ttactaaaaa aagtattaaa ttggtaattc
      961 gctgatggca tattttccgc gagcaacaca tcatttttta tgagatcact gtcagcattt
     1021 attgctaata tcagatgcaa gtagatggta tcccatgtag acaactcacc cttacgactg
     1081 gaatttaata tcgcgaaaaa ttccctcagt gaatccagga aattatcact ttgatttagt
     1141 tgtgtgcctg gaaatgatga tgcttgatca gatatattgt ttatcatatt aaaaatcacc
     1201 caagaaaaac gataatccac cgaactcctc gtgctttata attataaaag cacaacgcct
     1261 tttatattca tattaattaa cgagatactg aaacaactta atttattctt cgctaaaatt
     1321 cattagctta aattctttag ccacaattgt agcataaata atattatcga gagtcaaagc
     1381 tgggactatc aaatttcgct cattttcaaa aatacaacgt aattttggca atcatcttaa
     1441 tcgcaggatc tcttatcgca tgtgaaaaat tttgtaatga ttagacaata accgcgttca
     1501 tttatcacgc gttataaatg tgaaactggc ttcttgcaga tgctcttaac ctctacagga
     1561 ggatttatac caccatttca gaactatcat atttacctta aatgatcatt tcagccatat
     1621 aacacgctca acctcacaaa tggtttacac tgtctattgt ttgggtaatt taagtaagtc
     1681 agctacgagc aaccttgagt ggatagccag caatggactc aaacccaatc aggtgttcag
     1741 attgtttcag aattgtatta aaaacaatgt attaaataat ctgatagcgt ttggtcgtag
     1801 tgtgacgcag acagcaagct aataaggaaa tgattatgcg ctaccgttca ctatctctac
     1861 ttattccatg tgcactgctg ctgagtgcct gtaccaccgt cacgccagct tataaagata
     1921 acggtccccg caccggttcc tgtgtccagg gggggcctga cagcgtggct caacaattct
     1981 atgactaccg cattcaacat cgcagcaatg acattaccgc cctgcgcccc tatttgagtg
     2041 acaaactggc aacccttctt agcgatgcca gccgcgataa tagtcatcgt gaattgttaa
     2101 gcagcgatcc gttctccagt cgaaccacct tgccagatag cgcccacgtt gccagtgcat
     2161 cgactatccc taatcgtgat gcccgtaaca ttccattgcg cgtagatctc aaacagggtg
     2221 accagggttg gcaggatgaa gtgttaatga ttcaggaagg ccagtgttgg gtcatcgacg
     2281 atgtgcgcta cttaggcggc agcgttcatg ccacggcggg tacgctccgt cagtccatcg
     2341 agaatcgcta acgccttgat caaccaataa accccgtaaa ttgtccggca tttcgtgagg
     2401 aatgccgaca tttatgctcg ccgaccaccg cccccgttat tttgtgctat gtttattgaa
     2461 taatgcgctt tgcttttaac ttttaaagca gaaatattgc ataattattc tgtcaaaggt
     2521 actatcttcg a
//
LOCUS       DAZOYL010000393         2516 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZOYL010000393 DAZOYL010000000
VERSION     DAZOYL010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.397"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            81..1424
                     /gene="argG"
                     /locus_tag="ACHUOY_003934"
     CDS             81..1424
                     /gene="argG"
                     /locus_tag="ACHUOY_003934"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR0684557.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1432..>2516)
                     /locus_tag="ACHUOY_003935"
     CDS             complement(1432..>2516)
                     /locus_tag="ACHUOY_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR0684558.1"
                     /translation="ATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDTY
                     VLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTADS
                     VLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFDE
                     LLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIHY
                     TDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWYS
                     PVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDANH
                     DVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT          661 a          596 c          618 g          641 t
ORIGIN      
        1 aactcattta ttttgcataa aaattcagtg agagcggaaa tccaggctca tcatcagtta
       61 attaagcagg gtgttatttt atgacgacga ttctcaagca tctcccggta ggtcaacgta
      121 ttggtatcgc tttttccggc ggtctggaca ccagtgccgc actgctgtgg atgcgacaaa
      181 agggagcggt tccttatgca tatactgcaa acctgggcca gccagacgaa gaggattatg
      241 atgcgatccc tcgtcgtgcc atggaatacg gcgcggagaa cgcacgtctg atcgactgcc
      301 gcaaacaact ggtggccgaa ggtattgccg ctattcagtg tggcgcattt cataacacca
      361 ccggcggcct gacctatttc aacacgacgc cgctgggccg cgccgtgact ggtaccatgc
      421 tggttgctgc gatgaaagaa gatggcgtga atatctgggg tgacggtagc acctataaag
      481 gaaacgatat cgaacgtttc tatcgttatg gtctgctgac caatgctgaa ctgcagattt
      541 acaaaccgtg gcttgatact gactttattg atgaactggg cggccgtcat gagatgtctg
      601 aatttatgat tgcctgcggt ttcgactaca aaatgtctgt cgaaaaagcc tactccacag
      661 actccaacat gcttggtgca acgcacgaag cgaaggatct ggaatacctc aactccagcg
      721 tcaaaatcgt caacccaatt atgggcgtga aattctggga tgagagcgtg aagatcccgg
      781 cagaagaagt cacagtacgc tttgagcaag gtcatccggt agcgctgaac ggtaaaacct
      841 ttagcgacga cgtagaaatg atgctggaag ctaaccgcat cggcggtcgt cacggcctgg
      901 gcatgagcga ccagattgaa aaccgtatca tcgaagcgaa aagccgtggt atttacgaag
      961 ctccggggat ggcactgcta catatcgctt atgaacgtct gttgaccggt attcacaacg
     1021 aagacaccat tgagcagtat catgcacatg gtcgtcagtt gggccgtctg ctgtaccagg
     1081 ggcgttggtt tgattcccag gcgctgatgc tgcgtgactc tctgcaacgc tgggttgcca
     1141 gccagatcac tggtgaagtt accctggagc tgcgccgtgg gaacgattat tcaatcctga
     1201 ataccgtctc agagaacctg acctacaagc cagagcgtct gacgatggaa aaaggcgact
     1261 cggtgttctc gccagatgat cgtattggtc aattgaccat gcgtaacctg gatatcactg
     1321 atacccgaga gaaacttttt ggttatgcca aaactggcct gctctcctcc tctgccactt
     1381 caggcgtgcc gcaggtggag aatctggaaa acaaaggcca gtaattcgac atcaaccctg
     1441 cccttcaggg gtggggttac cttgcttatc ctctgtaaac tcctttctga gcatcacata
     1501 atcgaaaaca tcatggtttg catccacaac ccgtgagtct cctttatagt gtgcaatcac
     1561 ttcctccagc gttttcggtt gttccggctt tgttaccccc atccacgcat taataaggta
     1621 attattgtaa gctgtcgaaa agatgttgtt ttccgttgta cgatccacgc catccccaag
     1681 aacggggcta taccagataa acatcgggac atgatatgcc tgctggctag cctccctgcc
     1741 tccatgaaaa tagacgttct tcttcgtagg gtcacgttcc aggccgtggt cggcaaaata
     1801 catgaccgag gcgcggcgat cttttaataa ttcaaaaacc tgacctagca aactatctgt
     1861 gtaatgaatg gagttgtcat agcaggcatc ctgatcgtcc tgcggttgaa acacggcgct
     1921 ggattgcgga taggcgctac aagccggttc atggcttcca tttaaatgaa gaacaatcag
     1981 tttcttttgc tgcgtatttt gctgtaatgc ttgcgataaa tgcggcaaca acaattcatc
     2041 aaatcctctg acataaactg tttccatggc gcgcatggcg atactggtaa ctgctgtacc
     2101 attctgccga aaagcggatt gcgagcttag ccagaaagtc tgaaatcctg cttgattagc
     2161 catattaata atgttgtcgg ggtaattatg aatgtcatga ctcaaaacag aatcagcagt
     2221 taaagaaagg ggaaccgaca gcgcagtgta aggtgcgccg cttattgatt gattaaacag
     2281 tttgatctgt tttctttgtg cttcaacttg cggtgtcgta gagcgtgtat atccatacaa
     2341 agacatattg tcgacacgta cagactcccc cacaatcaac acgtaggtat caatacctgt
     2401 atccctgact gataattgaa aatacggcac cgtgtttgca attgagagta atctttgatg
     2461 ctctttcgct gctaaagcaa aataattgag attgaaaaac ggcgtatagg tagcaa
//
LOCUS       DAZOYL010000394         2504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZOYL010000394 DAZOYL010000000
VERSION     DAZOYL010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.398"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            278..421
                     /locus_tag="ACHUOY_003936"
     CDS             278..421
                     /locus_tag="ACHUOY_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR0684559.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            434..643
                     /gene="cspE"
                     /locus_tag="ACHUOY_003937"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             434..643
                     /gene="cspE"
                     /locus_tag="ACHUOY_003937"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0684560.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            809..1618
                     /gene="rlmA"
                     /locus_tag="ACHUOY_003938"
     CDS             809..1618
                     /gene="rlmA"
                     /locus_tag="ACHUOY_003938"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR0684561.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1615..2181)
                     /gene="mntP"
                     /locus_tag="ACHUOY_003939"
     CDS             complement(1615..2181)
                     /gene="mntP"
                     /locus_tag="ACHUOY_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR0684562.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          662 a          589 c          605 g          648 t
ORIGIN      
        1 gatattgcac gcactatgta ataacttctc ccactggcct ggaacaactg aacttattga
       61 actatgttag aaaatacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat
      121 gatatatcgg cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga
      181 tttgtgcgtc tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga
      241 taacagattt cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga
      301 agtcctgagt aaacacgttg acgttgaata ccgcttctct gccgagcctt atattggtgc
      361 ctcatgcagt aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta
      421 aggaattttt caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg
      481 ttttggcttc attactccgg ctgatggcag caaagacgtg ttcgtacact tctccgctat
      541 ccagggtaat ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga
      601 cggccagaaa ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg
      661 aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat
      721 gctttatctt cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg
      781 cggcctttag tatcctgccg gagttgtcat gtctttttcc tgtccacttt gccatcagcc
      841 tctttcgcgt gaaaaaaaca gctatatctg tccccagcga catcagtttg atatggcgaa
      901 agaagggtat gtcaatctgc tgtccgttca gcataaacgg tctcgtgatc cgggcgacag
      961 cgcggaaatg atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg
     1021 tgatgcaatt gtcgcccaac tgagggaacg gcttgatgat aaggccacgg cggtgctgga
     1081 tattggctgt ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac
     1141 cacgtttggt ctggatgttt cgaaggtagc gataaaagcg gcggcgaaac gctatccgca
     1201 ggtcactttt tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc
     1261 cataatacgt atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg
     1321 cggctgggtc attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat
     1381 ttacaatgaa gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca
     1441 gcagagtgcg gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt
     1501 gcagatgacg ccgtttacct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa
     1561 agaagtgttc gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc
     1621 gtggaagtgc gtccagagga tctggacgcc gatgccgatc agtaccagcc cgccgagaat
     1681 ttctgctttt ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgttga
     1741 cataatcaag gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgcaggaa
     1801 agcaagacca acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag
     1861 tagccagaaa ccgtgtcgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc
     1921 ctcaataatc attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtggttcca
     1981 ttcaaggaca aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt
     2041 ttcgacggca ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag
     2101 ggtggcacct ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag
     2161 aacagtagca gtgatattca tgacaatgtc ctgaccgggg tatccataaa acacatcatc
     2221 gcccccagca aacatgcata catgaacaac aagccgtgat ggttttcgcg ttcatgcagg
     2281 catagcgatt gatgtgcata tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca
     2341 cgttttgcaa cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc
     2401 aaatgagggc gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat
     2461 ttcattgctt tattgataac gattttcatt tggatttata tatc
//
LOCUS       DAZOYL010000395         2461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZOYL010000395 DAZOYL010000000
VERSION     DAZOYL010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.399"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..82
                     /locus_tag="ACHUOY_003940"
     CDS             <1..82
                     /locus_tag="ACHUOY_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684563.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHUOY_003941"
     CDS             complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHUOY_003941"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR0684564.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1028..1486
                     /locus_tag="ACHUOY_003942"
     CDS             1028..1486
                     /locus_tag="ACHUOY_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR0684565.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1669..2193
                     /gene="aroL"
                     /locus_tag="ACHUOY_003943"
     CDS             1669..2193
                     /gene="aroL"
                     /locus_tag="ACHUOY_003943"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR0684566.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHUOY_003944"
     CDS             2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHUOY_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR0684567.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
BASE COUNT          608 a          613 c          664 g          576 t
ORIGIN      
        1 tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa
       61 ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct
      121 gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc
      181 agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc tttcagtgcc
      241 cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca
      301 aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg
      361 ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc
      421 ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta
      481 tcttcggggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg
      541 ggagtgttcg gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg
      601 tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg
      661 atttcgctaa gcactttaat catgatgcca ggtttaacgg cggcaaaaat gatgtcggcg
      721 atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg
      781 gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg
      841 gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc
      901 ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca
      961 taaacagaac tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga
     1021 tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt
     1081 gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg
     1141 cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga
     1201 taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt
     1261 ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac
     1321 gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag
     1381 tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccagg cctttgccgc
     1441 ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat
     1501 ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt
     1561 aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct
     1621 catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct
     1681 ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat
     1741 tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg
     1801 gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg
     1861 ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg
     1921 gaatttaatc gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta
     1981 tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg
     2041 ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc
     2101 gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat ttctgaaatt
     2161 cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca
     2221 tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat
     2281 agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga
     2341 tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc
     2401 gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt
     2461 c
//
LOCUS       DAZOYL010000396         2458 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZOYL010000396 DAZOYL010000000
VERSION     DAZOYL010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.400"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(73..2352)
                     /gene="maeB"
                     /locus_tag="ACHUOY_003945"
     CDS             complement(73..2352)
                     /gene="maeB"
                     /locus_tag="ACHUOY_003945"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR0684568.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT          558 a          706 c          613 g          581 t
ORIGIN      
        1 agctttacta atatattgca gccaaaccgc ttaccctgaa tattcagggt aagcgtgaga
       61 gttaaaaaag aattacagcg gttgggtttg cgcttcaacc acggccagcg ccaccatgtt
      121 gacgatacga cgcaccgatg cgatcggcgt taacacgtga accggtttcg ccacgcccat
      181 cagcaccgga ccgacagtca caccttccga gctggaaacg cgcagtaagt tgtaactaat
      241 gcgggcagct tccatgttcg gcatcaccag aatattggca gaacctttca aagggctgtc
      301 cggcatacgg tcgttgcgaa tcgcttccac cagcgccgca tcgccgtgca tttcaccatc
      361 aatcatcagt tctggcgcac gttccctgac cagttccagc gcctgacgca ttttgctcga
      421 cgacgggcag tcagaagaac caaagttgga gtgcgacaac agagcaacgc gcggttcaat
      481 accgaaacga cggacagttt ctgccgccat caaggtgatc tccgccagct cttctgcgtc
      541 tggttcgtca ttgacgtagg tatcggcaat aaaggtgtta ccactcggca gcagcagcgc
      601 gttcattgca cctgcggtgt gaacgccatc gcgataacca aagacatttt tcaccacgct
      661 aaaatgttca tgataatcac ccaccgtacc gcaaatcatt gcatcggctt cgccacgctg
      721 aaccatgatc gcgccgatca ctgtcgggtt actgatcagc gcccgctgcg cctgttcctg
      781 agtgacgccg cgacgcttca tgatctggaa gtattcggtc cagtactctt taaagcgcgg
      841 atcggattcg ttattgacga tctcaaaatc aacgcccgct ttgatctgca agcccagttt
      901 ctgaatgcgc atttcgatca cgttcggacg accgataagg atcggtttcg ccagtcccag
      961 cgttaccagt tcctgagtgg catgcagaac gcgcgcctct tccccttccg gcagaactac
     1021 gcgcttcggc gctttgcgag cctgggagaa aatcggcttc ataaacaggt tggttttgta
     1081 aacgaactca gtcagcttgt cgatgtagac gtcgaaatca gcaatcggac gcgtcgccac
     1141 gcccgactcc atcgccgctt tcgcgaccgc aggagcgatc ttaacgatca agcgcggatc
     1201 aaacggtttt ggaatgatgt attccggacc aaagctcaga tcctgatcgc catacgctga
     1261 agccaccact tcgctctgtt ccgcatgggc cagttctgca atcgcgcgta ccgccgccag
     1321 tttcatctct tcgttgatgg cggttgcgcc aacgtccagc gcgccacgga agatgaacgg
     1381 gaagcaaagg acgttgttga cctggtttgg atagtcagaa cgaccggtgc agatgatggc
     1441 atccggacgc acttctttcg ccagcggcgg cagaatttcc ggttccggat tcgccagcgc
     1501 cagaatcatt ggtgcccgag ccattttctt caccatttcc tgggtcagca ctttcgggcc
     1561 ggaacagccc aggaaaatat ccgcaccttc aatcacatca tcgagggtac gtttgccgtc
     1621 atccaccacc gcatatgcgg ctttggtttc cgccatgttt ggctcacggc cttgatagat
     1681 aacgcctttt gaatcgcaaa ccacgatgtt atgtttttgc agacccagcg ctaccagcag
     1741 attcatacag gcgattgctg cggcacccgc gccggaaacc accatccgca cgtcggagat
     1801 gtttttctcc accacgcgca agccgttgag gatggcggca gtgctgatga ttgccgtgcc
     1861 gtgctgatcg tcgtggaata ccggaatatt catccgctcg cgcagtttct gttcaatata
     1921 gaaacattct ggcgctttaa tgtcttcgag gttgattccg ccgaaggtcg gttcgagcgc
     1981 agcgacaact tcaataaatt tgtccgggtc gagttcatca acttcaatgt caaatacatc
     2041 aatcccggcg aatttcttaa acagaacgcc cttgccttcc atcaccggtt tgccagccag
     2101 cgcgccaatg ttgcctaacc ccagcaccgc cgtaccgtta gagatcaccg ccaccaggtt
     2161 accgcgggcg gtatatttgt aggcttttaa cgggtctttt tcgatttcaa gacaaggtgc
     2221 ggcaacgccg ggtgagtagg ccagcgccag atcgcgctgc gttgccagag gcttggttgg
     2281 agaaacctgg attttccctg gaactggaaa ttcatggaaa tcaagtgcac tttgttttaa
     2341 ctggtcatcc atttggttgt tcctttcacg taacgttcac aaataaagtg tgtgggcaac
     2401 accccctgcc cacaacgtgg cgcacattat taccttgccg gagtctacag actttgag
//
LOCUS       DAZOYL010000397         2437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZOYL010000397 DAZOYL010000000
VERSION     DAZOYL010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.401"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            221..337
                     /locus_tag="ACHUOY_003946"
                     /pseudo
     CDS             221..337
                     /locus_tag="ACHUOY_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(442..518)
                     /locus_tag="ACHUOY_003947"
     tRNA            complement(442..518)
                     /locus_tag="ACHUOY_003947"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Arg,seq:tct)
     gene            complement(532..608)
                     /locus_tag="ACHUOY_003948"
     tRNA            complement(532..608)
                     /locus_tag="ACHUOY_003948"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Arg,seq:tcg)
     gene            complement(616..691)
                     /locus_tag="ACHUOY_003949"
     tRNA            complement(616..691)
                     /locus_tag="ACHUOY_003949"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Ile,seq:cat)
     gene            complement(732..1790)
                     /locus_tag="ACHUOY_003950"
     CDS             complement(732..1790)
                     /locus_tag="ACHUOY_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR0684569.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1898..2138)
                     /locus_tag="ACHUOY_003951"
                     /pseudo
     CDS             complement(1898..2138)
                     /locus_tag="ACHUOY_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2363..>2437)
                     /locus_tag="ACHUOY_003952"
     CDS             complement(2363..>2437)
                     /locus_tag="ACHUOY_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0684570.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          585 a          698 c          560 g          594 t
ORIGIN      
        1 tagcggtcat gtgatgccgg tttcccggta actcagcacc ggtatctgag tcaacgtttt
       61 ctctactggg tcatttccga tacgccctgc ctgctgacag gctttcatca catctgaaaa
      121 tatagcaccc tgactgatac tgtagtacct aaggttccag aaactgtgat gtatccggca
      181 cagaaaagcc cctccggaga gggctggaga gtggcgctat gtgccattgc atgatgccgg
      241 gtgcctcccg gtgaattcag taccagcacc tgaatccgcg attatcccat atacctactc
      301 gctgattgcc cctccgcaca gggggattca ccatgccagt ttcttttaac aaactccccg
      361 caaaacagac aactgtcaac cgtctgaatt gtgagacatt taaaaaaaag gcccgcaaaa
      421 gcgagccagg gaaaataagt gtggcgcgtt gtactggatt cgaaccagtg accgattgct
      481 tagaaggcaa ttgctctgtc cggctgagct aacaacgcat gatgcagata atggaccgcc
      541 atcggggact tgaaccccgc gcagccagct tcgaaggctg gcgctctttc ctgatgagct
      601 aatggcggta tgtgatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag
      661 tcgctcgctc tcaccactga gctaaagggc cgggagccgc ataataacga cgcgtaatta
      721 attcttcaat atcatccgtt ctggctgact aaatcctgta cttcccgaac cgtctgctca
      781 aaacgttcag tctccagctc aacgccaatt gcacgacgcc cgagcgccag tgccgctttc
      841 actgtcgaac ccaaccccat gaaaaaatct gcaatcaggt cacccggacg actgctagcg
      901 ctgattatct gctgcagcat ttctgccggt ttttcgcacg gatgtttccc gggatagaac
      961 tgcaccggtt tatgcgtcca cacatcggta tacggcacct gcaccgtcac gccaaaatac
     1021 cgccgcagat gtttatattc actcagcagc tccgcatact gccggttcag tgaggtatac
     1081 gtatccacca gctggtggtg gagcttttcc agttcaccac gccgatgctt ctcttctgcc
     1141 acccgggcaa acagcgcctg taatttcaga taatcgcttt cattcggcaa ctgccactga
     1201 ccggcactga accagtgcga caccatgttt ttctttcctg tggcatctgc aatctgtttt
     1261 gccgttatcc ccagggcagc tcgcgcatca cgaaagtaag aaatcagcgg ggccatcaca
     1321 tgctgtttca gtgcactgcc cttcgccgca tacccggcat ctttcggacg atacggcccc
     1381 tgataatgtt ccgcgaacag aatgcgctct gtggcgggga aatacgcccg caggctttcc
     1441 ttgttgcatc cgttccagcg tccggacggc ttcgcccaga taatatggtt cagcacactg
     1501 aagcgttcac gcatcatgat ttcgatatca gatgccaggc gatgaccaca gaacaggtaa
     1561 agacttccgg caggtttcag cacccgccag aactgcgcca gacactggtc cagccacttc
     1621 aggtaatcat cgtcgccctt ccactggtta tcccagccct caggcttcac tttaaagtac
     1681 ggcgggtccg tgactatcag gtcaacagaa ttttcgggta acgaccggat aaattccagg
     1741 cagtcggcgt tgattaactc acaactggat atttttacag tattaagcat ggatcattaa
     1801 gcctgtctct gataggctca ttctgctttt gcgcaaagca gtgggcctga ggtttgcttg
     1861 tgaacccaac gcatgagcag atggctggtg ggtgccccta acacccacca gccgcccatt
     1921 taccacaaat aaaaaagcct tcactgcgga aggcgtctgt aacaaccgaa ctgatagtct
     1981 gccagacccg ccataaccag ctgggtcagt attaactggc agcgttcgcg tgaaaggtaa
     2041 gtattctgcg ctatctcccc gactgtcgcc ggttcggtaa cgcttaattc attaaacacc
     2101 actctggcgg tttctgtcat atcctgctgt tttagcatgt cttttccttt tccggttaac
     2161 gtgacacacc aataactctt gtcgaaaaag ccagcaagct gaaagacagg tattcaccgc
     2221 caccagcgcg tttactgtac tgacgcgatt tcagtcataa aaaacccgct cggcggcggg
     2281 ttgtagaaac tcttctaacg tcaggcataa aacgcccatc gttatgacga atttaccaca
     2341 gattccggaa aaatcaacct tgttacctag ttaccttttt taactgccgc tcagcccatg
     2401 cttcttcaat atcaaaccgg gtcaccagcg catcata
//
LOCUS       DAZOYL010000398         2433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZOYL010000398 DAZOYL010000000
VERSION     DAZOYL010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.402"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUOY_003953"
     CDS             complement(<1..304)
                     /locus_tag="ACHUOY_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0684571.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
     gene            complement(343..630)
                     /locus_tag="ACHUOY_003954"
     CDS             complement(343..630)
                     /locus_tag="ACHUOY_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684572.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(880..1035)
                     /gene="hokD"
                     /locus_tag="ACHUOY_003955"
     CDS             complement(880..1035)
                     /gene="hokD"
                     /locus_tag="ACHUOY_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0684573.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1263..1928)
                     /locus_tag="ACHUOY_003956"
     CDS             complement(1263..1928)
                     /locus_tag="ACHUOY_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR0684574.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2097..>2433)
                     /locus_tag="ACHUOY_003957"
     CDS             complement(2097..>2433)
                     /locus_tag="ACHUOY_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684575.1"
                     /translation="ELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVRVDEKERQ
                     VRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIERNAKARI
                     RRATKSRTE"
BASE COUNT          611 a          627 c          539 g          656 t
ORIGIN      
        1 gctctgcggc ttcgctcaga gctggcgtat gttcccggcc tactgattca cacaggtaat
       61 caacgaaacc ggacaccagt tttcgttcgc gaggcgtgat cgccccaccg accggagtcc
      121 agtaatcgaa acccagttgc aggagtttga aaaaacgctt gtggaatgcg tagttacaca
      181 cacgcttaaa gtctgcgtgt atccactcac ctattttgat ttgatgcaga aaatcgcaac
      241 tctccggcgt cgccgggaga agtaatccgg aagaggtttg tttgaccagt tgtatatgtg
      301 ccatcggctt tctccggtgg cacggtgtta cacagcagga gttcaatcct gctcaagatt
      361 gtagatgagt ttattcttca gcaaaagcag aaaaccagcc ttaaaaccaa tctctttcaa
      421 aacccgtaat gatgtgacaa attcgtcctc acgcaaaata aaaccgtccg tcagaagtcc
      481 attacaaaaa taaaataaca cagcaccgct cttcctttgt tgagattgca aacatctaat
      541 gcggcaatgg ctgacaatcg ctccattctc aacgcgcaca gcatagaggc cattttcact
      601 aaaaatttca cgcaattctt cgattttcat cttcagaatc cttccagata aatagctctc
      661 ccctgttcgg ggtccatccc tcttctccct gcgcgctact taagtgagtc gattctatct
      721 gcgaaggtgc gcgaatcaaa ttcaccggaa ataaacaaca aaaaaaccgc cgaaacgctg
      781 agagatcaca gcgatgggcg ggttaagtgc gggtgcgttg aggatgcctg acacatcaga
      841 ggtgggcggg gatgggatca gctccccgcc cggtcactct tacttcctgg attcgtagtc
      901 tacgaagaca gcgacctccg tctggccggt tcggattcgt acctcacaga ggtctttcct
      961 cgttaccagt gccgtcacta tgacggttaa acagatgacg atcagggcga ttaacatcgc
     1021 cttttgctgc ttcatagcct gcttctcctt gcctttcggc atgtaagagg ctaacctaca
     1081 tgtgcaaagc atgaaattgg cctcagatta atgttaagcg tcttgccgga cgcgtaatgt
     1141 taactggggc ttttctctat ctgccttttg gtgttcatgc ctgaagcaga tagcctcaag
     1201 cacccgcagt cattctactt acctcgccaa tatgaaatca atcagaaagg tgccccataa
     1261 aatcactcct tctcttcttt accgtagtgg agttgaccaa ttttgataag agggcgtccc
     1321 tgagatttgc ggtgtagatt ggtatcgcgc agagaataca cacagccaca atattcctgc
     1381 tgatagaatt tttcgcgctt gctgatttca atcatacggg acgagccgcc ctgcttgcgc
     1441 cagttataat cccagtacac catgcccgga taatgcgcga cggctcgccg cccacagtcg
     1501 ttaacctgct gcatattttt ccagcgtgaa atgcccagtg aactgctgat cacactgaaa
     1561 ccattttcag cggcgtacag cgctgtccgc tcaaaacgca tgtcaaaaca catggtgcaa
     1621 cggatccctc gttcgggctc ccattccatt cctttggctc gttcaaacca gttgtctgtg
     1681 tcgtaatcag catcaataaa cggcacgccg tgttgttcag caaagcgaat attctcatcc
     1741 ttacgaatta aatactcttt ctgaggatga atgttcgggt tgtagaaaaa gatggtgtag
     1801 tcgattcccg aggcctgaag cgcctccatc acctcaccgg aacatggagc acagcaagag
     1861 tgcagtagta gtttgtttgc cccgtttggg agctccaatt tgggccgttt gaaatcagca
     1921 actgtcataa atatgtttat tggggtcata aaaatatcaa agagtgtagc attagaacgg
     1981 ggctatcgga aacagatgtg tgactgctcc ccgccctttc gggtggtctc ctgatgattt
     2041 gagggtgcag aaatccctcc ggttaaggat taaattttta acagtgctaa atttaattat
     2101 tcagttctgg attttgtcgc cctgcgtatc cgcgctttcg cgttacgctc aatctgtatc
     2161 agcttttcta tatttctccg cctttcctgt tcctcctggc gcaatagcct tacatcatct
     2221 gccagtctgg tttctctttt tgccacagag agcatccagt caaacggctc cacaaccgca
     2281 ccgcagattt tacagcggac ctgacgctct ttttcgtcaa cccggacaga ggcgtgatgg
     2341 caatatggtc tttccgatgg ttcataaaga aaattaacct gattacgtgg gtcatcctct
     2401 tttaccggaa ataaaacgat attgcttaac tca
//
LOCUS       DAZOYL010000399         2402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZOYL010000399 DAZOYL010000000
VERSION     DAZOYL010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.403"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            75..164
                     /locus_tag="ACHUOY_003958"
                     /pseudo
     CDS             75..164
                     /locus_tag="ACHUOY_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="ACHUOY_003959"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="ACHUOY_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFR0684576.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..1962
                     /gene="hipA"
                     /locus_tag="ACHUOY_003960"
                     /pseudo
     CDS             641..1962
                     /gene="hipA"
                     /locus_tag="ACHUOY_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2146..2247
                     /locus_tag="ACHUOY_003961"
                     /pseudo
     CDS             2146..2247
                     /locus_tag="ACHUOY_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          666 a          505 c          618 g          613 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagccc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaataacatc
     1681 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     1741 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     1801 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     1861 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     1921 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     1981 tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta tgtttgtgcg
     2041 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     2101 tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac gaatatactg
     2161 ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc aaacgctgat
     2221 gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg tcatctgaaa
     2281 ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caattcacca atgtgaatct
     2341 cattattaat gaggagtggt ttgtatttgg agcgctatga aaaatcacat aatatctaaa
     2401 ct
//
LOCUS       DAZOYL010000400         2401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZOYL010000400 DAZOYL010000000
VERSION     DAZOYL010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.404"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..856)
                     /locus_tag="ACHUOY_003962"
     CDS             complement(<1..856)
                     /locus_tag="ACHUOY_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR0684577.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
     gene            complement(913..2091)
                     /gene="allB"
                     /locus_tag="ACHUOY_003963"
                     /pseudo
     CDS             complement(913..2091)
                     /gene="allB"
                     /locus_tag="ACHUOY_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(2010..>2401)
                     /locus_tag="ACHUOY_003964"
     CDS             complement(2010..>2401)
                     /locus_tag="ACHUOY_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR0684578.1"
                     /translation="LTGTRQALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWAD
                     KIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDP
                     YFLNRVVATGKVMKPVLAQRQKVVSPP"
BASE COUNT          623 a          664 c          587 g          527 t
ORIGIN      
        1 atccggtcgc cacaaagctg cgacgataac gcgtattccc cgcgccctgc tgcggataaa
       61 aaacatccgt gccccgaatc gcaccgtagg tattgctgat atttaccaga cctgtaatca
      121 ccgccgtcag aataattccc ggcgacaaag caccgccact gccgagcggg aaccactgcc
      181 agtgcaactc accggagagc gaatgcgaag aaggaaagca aaagtaccac aacaaccagc
      241 cggttatggt gccgaccagc aggccataac gggcaaaacg ttgcggcaga aagataatca
      301 tcgccagtac caggcacatc accgccaccg agagcgcgaa cggtggtaac tgaattttaa
      361 aatatgggtc ggctatgcca aacggcagcc cgagcatacc tttgaaaaag atagtggtca
      421 gctgtgcgcc cagcatcaac ataaacaaga ccatcaccga cggcgtaaat aaccgtgcca
      481 ggcgatggcc taatccgcta aaaccaatca acatcgtcag cacgccggag agtgcaatcc
      541 ccaccgccag actggtagcg atatcgttga tcggtgtccc gcgcgatgct tctccaaggg
      601 tgatagtaag gatggttccc caccacaggc cacccggccc ttccataata gcgcgacgat
      661 gaccgcagaa cgcctgagcg aagcaggcca gtgcggtagc aagaaaagca tattgcgtga
      721 gcgtaagcag gctactttgc ggcaactgaa aagcagaaag tagcgtagga ggaaccacaa
      781 ccgtgttgca aaaaataaag aaaaaccact gaaatcctga taacaggctt tcgcggctga
      841 ctgcaaaatt gaacataaac acaccttaac cggagaatgc ccgccgcaag gacgggcatt
      901 gcaggggcca gattactgct gatgtttaag gataaattga cctttcggcg caacagggaa
      961 gccctgttca atgtcgtaaa tcacatcacc acgtaagatg gttttcgtga tacgcgcgcc
     1021 aatggtacgg ccaacatacg ggctgacttt gtggcgatat tccagatcgt cattggtaag
     1081 aacatagctg ctattcggct gaatgaagac gaagtcggca tcttttcctg gggcgatacg
     1141 gcctttttgc tgcagaccga aaatatctgc tgcgttagtc gccattaatt tgccgaacat
     1201 tggcagagac attccgcgtt tctgtaccgc ttcatcgaac atcacgtcca tacagttttg
     1261 cagaccggca ataccgcccc atgcttccat gatgttgccg gctttcattt ccggagggca
     1321 tggtgagtgg tcggaaacca ggcagtcgat ttcaccgtta aacagttttt cccacatgcc
     1381 tttctggttt tccagatcgc ggatcggcgg tgaacacttc gccagagtac cgatttcttc
     1441 gaactgatcg gtatccagta caaagtaatg cgggcaggat tcacaggtaa catcctgacc
     1501 ttcctgacgt gcacgagtca cttcttcaac actttccggg ctgctgatat ggcaaacgtg
     1561 cagacggcaa ccggcaactt tcgccagata cagcacgcgg cgaatcgctt ccacttcggt
     1621 aaataccgga cgcgaagcca catagtcatg ggcagttacg cgaccttcac gtttcgcttc
     1681 ttcgcccggt gcgtcacaga tcagcgcgtt ttcgcagtgc accaacaccg gctgccccag
     1741 ttcgcccagc ttctgcgcac ctttgaagaa ctgccagtcg tttacatcac ggaagtcgtt
     1801 gtcgataccg cgatcgccac aggtcgcaac gaagcatttg aagccgacaa cgcccacttc
     1861 atccagctca tgcagacggt cgatgttgta agacaccagg ccaccgagtt gtgccgcatc
     1921 gatagtcagc ttgcctttag cggcatcgaa cttcagttca attgaagcgc ggtcaaccgt
     1981 tgcaggcagc tggttgagcg gcatttcgat catggtggtg ataccacctt ttgccgctgc
     2041 gcgagtaccg gtttcataac cttcccagtg gctacgaccc ggttcagaaa gtacggatcc
     2101 atccagcgta aaccggcttc cgtggtggtt tggggtttat attcacaccc aacccaaccg
     2161 ttgtagtcag aattttcgat taccttaaag agataatcat aattaatttc tccggtcccc
     2221 ggttcgccgc gatgcggatt atcagcaatt tgcaggtgac caattttatc ggcccactga
     2281 gtcatggtgt tggttaattc accttccatc cgctgcatgt gataaatgtc atactgaatt
     2341 ttcaaattgc agcaaccaac atcatcaatc aatttcagcg cctgccgagt tccggtgaga
     2401 t
//
LOCUS       DAZOYL010000401         2381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZOYL010000401 DAZOYL010000000
VERSION     DAZOYL010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.405"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1511)
                     /locus_tag="ACHUOY_003965"
     CDS             complement(<1..1511)
                     /locus_tag="ACHUOY_003965"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR0684579.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINF"
     gene            complement(1532..2158)
                     /locus_tag="ACHUOY_003966"
     CDS             complement(1532..2158)
                     /locus_tag="ACHUOY_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR0684580.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT          615 a          620 c          504 g          642 t
ORIGIN      
        1 ataaagttga tatgggtatg cgtcatgcag tcacggttga acatacagtt aacaatggaa
       61 ccgacctgcg ctgacgcttc attggcatgg tgaaccggat agccaaacgg cgaccagagt
      121 ttatttttcg cgtagcccca gtactcttca cccaaattcc agcgttctgc gatggcatat
      181 gaaccatcag ccaggtgact cagctcaccc acacgatgcg ccagacggta gtaaaaatct
      241 ttaattttaa tgaagttaac gtcacccgct tccagttgat cccagcgaat ttccgcatac
      301 tcttcagcag gcagaacacg cttgaacaca cctttgctgt aacaataggt aaagtcgcga
      361 tgcaactgcc cgtagttaca ccataggccg tagtcatcga acagattcag acccaccagg
      421 ttaccaatca cacgaccatc gtctttatct tcaaaatctt tcggaccgtt cgggaagatg
      481 gtggtatgga caaagttggc tacacaagtg ttaccacctg tgctgggcac gccaaactct
      541 ttcacccgag gaatattcat ttgggtcata caacggatcg ggcaagagtg gcagccactc
      601 atttttactg tgtatttctc tgccgccgga cctaagtcaa aaacggattt ataggtacga
      661 aagccgaccg tgttctgatt gcctggcgga atttcccccg tttcaatcgg accaccttca
      721 gccgcgcccc aaaacagccc tttacgtgct gtccaacgtg acttgggatc tgaatactct
      781 gcccacgatt gcggcgtact tggcacgaca tggttgttat tcgcaccaat aagttcagtc
      841 atcatgtaat cgttgagacg cttcatctcc tgacgatcgg caatgttgac ccctttcgtc
      901 ccttcaaccg caatcgcttt caggtttttc gaacccatta ttgcgccagt tcccgcaccg
      961 ccgctgtggt tacggctatt caacatgcca gagagaggaa caaggttttc cccagcctga
     1021 ccaatagccg ccacacaggt ttccggactg gtcaatcgac aaatttcttc cgtcgttgcg
     1081 cgcgtccctt ttccccataa gaaatcggct ttttccaggc taactttgtc atctttaatc
     1141 ttcagccata ccggtgattt cgccttccct tcgataataa tgacgtcgta tccagcgaat
     1201 ttcatttgcg ctgcaaaaaa gccacccata tgggcatcga cgactaaatt tcctttggta
     1261 aaagtagaaa gtgaggtgat atttacgcga gaactacagg gggcaccaga tccagttaat
     1321 gggccggtag caaagactaa tttattcgct tcatcgaaag gtttcgtgcc tggcggtact
     1381 tcgtcataca taattttgta gccgaagccc atgccaccga caaaactttt aaacttactg
     1441 gaatcttcga gggtaatatt tcctgtcgtg agattgactc ttaatatatt acctgtccaa
     1501 ccgttagcca tgattttttc ctttgcaaga ttcacacagt aatatctttc cactcgataa
     1561 tttttaacgc ccctgttggg caggcgtttg cgcattcacc gcataacaca catttcgagg
     1621 attttttact ttcggtattt acggtggcca tcatccacgg gcatgccgta gtacaggcgc
     1681 tacagccaat acagcgttta tgatcgacgg taatacagcc ttctttctgc tgccaggtaa
     1741 tcgcaccaat cgggcagacg ttcatgcatt gcggctcttt gcattgacgg caggtgtccg
     1801 cggtatagtt gagatcgcca tacaggccgc cgccagagcc aaccccgttg tcgccaaaga
     1861 aataattgcg atggattttg atacgggaga agaatgtccc tactgagcca tcgttgaagt
     1921 tggtacaaga gatttcacag cggtggcaac ccgtgcatcg cgctcgctgg gtcaccagca
     1981 cccctttggg tgtatttatc aaccctactg tgccgctatc aatatcttcc tgtttgcaac
     2041 ccaaaagtga cagtaacgca ggggcaattg ttagaccagc aaggcctttt cctgatatac
     2101 gcaagaattc taatcgcgtt aagccaatat ctaatagtgg acgatcaacc gggttcattt
     2161 atttcgcatc ctcttcgcaa acacagtgct gacaattcag cagcctgata ttaagcattc
     2221 aactaatacg ctgctgactc tgtccccaac accagatatt ttccctaacc cgatagtgta
     2281 tgagaaaaga tttaacgccg ataaaccaat cctttatggt taggagaaaa aataatgtga
     2341 ttattctgag cccttaacat tgatcgttat caattaaaat t
//
LOCUS       DAZOYL010000402         2375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZOYL010000402 DAZOYL010000000
VERSION     DAZOYL010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.406"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1248
                     /gene="frc"
                     /locus_tag="ACHUOY_003967"
     CDS             <1..1248
                     /gene="frc"
                     /locus_tag="ACHUOY_003967"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR0684581.1"
                     /translation="STPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVT
                     RHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAID
                     HMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPL
                     VSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLD
                     KLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNW
                     ENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPC
                     APVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHT
                     AAVLQELGYSDDEIAAMKQNHAI"
     gene            1302..>2375
                     /gene="oxc"
                     /locus_tag="ACHUOY_003968"
     CDS             1302..>2375
                     /gene="oxc"
                     /locus_tag="ACHUOY_003968"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR0684582.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQK"
BASE COUNT          633 a          532 c          588 g          622 t
ORIGIN      
        1 tcaactccac ttcaaggaat taaagttctc gatttcaccg gtgtgcaatc tggcccatct
       61 tgtactcaaa tgctggcctg gtttggcgct gacgttatta aaattgaacg tcccggcgtt
      121 ggtgacgtaa cgcgtcacca actgcgagat attcctgata tcgatgcgct ttacttcacc
      181 atgcttaaca gtaacaaacg ttctattgag ttaaatacca aaacagcgga aggcaaagag
      241 gtaatggaaa agctgatccg cgaagctgat atcttagtcg agaactttca tccaggggcc
      301 attgatcaca tgggcttcac ctgggagcat attcaagaaa tcaatccacg tctgattttt
      361 ggttcgatca aagggtttga tgagtgttcg ccttatgtga atgtaaaagc ctatgaaaac
      421 gttgctcagg cagcgggtgg cgcggcatcc actacgggtt tttgggatgg tccgccgctg
      481 gtaagcgctg cagcgttggg tgacagcaac accggaatgc atttgctgat cggtttactt
      541 gctgctttgc tgcatcgcga aaaaacgggg cgtgggcaac gagtcaccat gtcaatgcag
      601 gatgccgtat tgaacctttg ccgcgtgaaa ttacgtgacc agcagcgtct cgataaattg
      661 ggttatctgg aagaataccc gcagtatccg aatggtacat ttggtgatgc agttccccgc
      721 ggtggtaatg caggtggtgg cggtcagcct ggctggatcc tgaaatgtaa aggctgggaa
      781 accgatccta acgcctatat ttatttcact attcaggagc aaaactggga aaacacctgt
      841 aaagccatcg gcaaaccaga atggattacc gattcggcat acagtacagc ccatgcccga
      901 cagccacata ttttcgatat ttttgctgaa atcgaaaaat acactgtcac tattgataaa
      961 catgaagcgg tggcctattt gactcagttt gatattcctt gtgcaccggt tttaagtatg
     1021 aaagaaattt cacttgatcc ctctttgcgc caaagtggca gtgttgttga agtggaacaa
     1081 ccgttgcgtg gaaaatatct gaccgttggt tgtccaatga aattctctgc ctttacgccg
     1141 gatattaaag ctgcgccgct attaggtgaa cataccgctg ctgtattgca ggagctgggt
     1201 tatagcgacg atgaaattgc tgcaatgaag caaaaccacg ccatctgata ttaacggggg
     1261 cttctggctc ccaatttata aaaatttcga ggttattaat catgtcagat caacttcaaa
     1321 tgacagatgg tatgcatatc atcgttgaag cattaaaaca gaataatatt gacactattt
     1381 atggtgttgt aggtattcct gtgacggata tggcacgcca tgcccaggcg gaaggcattc
     1441 gttatattgg ttttcgtcat gagcagtcgg caggctatgc cgctgcggca agcggttttc
     1501 ttacccaaaa accggggatc tgcctgacag tttctgcgcc aggattcctc aatggtttga
     1561 ccgcattggc caacgcaacg gtaaatggtt ttccgatgat catgattagc ggctccagcg
     1621 accgcgcgat cgtcgaccta cagcaaggtg attatgaaga gctggaccaa atgaatgcgg
     1681 caaaaccgta tgccaaagca gcatttcgcg ttaatcagcc gcaggatctt ggcattgcat
     1741 tggcacgcgc tatccgggtc tctgtatcgg gtcgccctgg cggagtttat cttgatttgc
     1801 cagcaaatgt cctggccgcg acgatggaaa aagacgaagc gttaaccacg attgttaaag
     1861 ttgaaaatcc gtcgccagca ttattgccat gcccgaagtc agtcactagc gcaatttcgc
     1921 ttttagcaaa agctgaacgg ccattaatta tccttggcaa aggcgcggcg tattcacaag
     1981 ctgatgaaca gcttcgtgaa tttattgaaa gtgctcagat tccattcctg ccaatgtcta
     2041 tggcgaaagg gatccttgaa gatacgcatc cactttctgc ggcagctgcg cgttcgtttg
     2101 ccctggcaaa tgctgacgtt gtcatgcttg ttggtgcacg actgaattgg ttattggcac
     2161 acggtaaaaa aggatgggcg gcagatacac agtttattca actggatatt gaaccgcagg
     2221 aaattgacag caaccgcccc attgctgtgc cagtcgttgg tgatattgca tccagtatgc
     2281 aaggtatgct ggcagaactg aaacaaaaca catttacgac tccactggta tggcgcgata
     2341 ttttaaatat ccacaagcag caaaatgcac aaaaa
//
LOCUS       DAZOYL010000403         2291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZOYL010000403 DAZOYL010000000
VERSION     DAZOYL010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.407"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1258)
                     /locus_tag="ACHUOY_003969"
     CDS             complement(<1..1258)
                     /locus_tag="ACHUOY_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0684583.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLIERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLLN"
     gene            complement(1415..1624)
                     /locus_tag="ACHUOY_003970"
     CDS             complement(1415..1624)
                     /locus_tag="ACHUOY_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684584.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1647..>2291)
                     /locus_tag="ACHUOY_003971"
     CDS             complement(1647..>2291)
                     /locus_tag="ACHUOY_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0684585.1"
                     /translation="EHPLHTIQRQEHDKRIELEQELKMSPKVISFVSPEPQEMPDICK
                     TEALFELEREYYPALKSQRLRLDVAYDAVKNFEETSKPSEYDIAYEIKSNPFIYYEGN
                     FNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSGQFILMTDKTDEEILQLAEANLT
                     KHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIALMADIEQLTKISQKYSKAV"
BASE COUNT          641 a          489 c          377 g          784 t
ORIGIN      
        1 aattaagtaa attagtcagt gaccttgtaa agtctgttaa accttcctgt gaaccatcga
       61 acattaaggc cagattatta ttccatgaat tacttaagcg gcctaactgc gtctgagtag
      121 agttaataat cttttcccat ccaccagctt tatcaatcat ccttcccatt tcagcggata
      181 ctttaggaaa aacatcttta gaaagaacct taccgtcttt catggccttg tctaattctt
      241 ccgtagtcat cccaagactt ctggcaaata actgaaaagc acctgccatt ctgtcaccta
      301 attgaccgcg tagctcttca gcctgaatct ttcctttaga tgccatctgc tcgaaagctt
      361 ttaaaatccc gttaatttca tcggtgctta gccccattaa tctggatgta ccaatcacat
      421 tagaaaataa ggattctttc tggtctgcat tcatatcacc agcaccagcg gaaaacttag
      481 caaaactgct taatgtttca atctggtcta caccactact atcagcaaga ttcttaacaa
      541 attctgtagc tcgtttagca ccttcatcac ctaaaacgaa ttgtgccgct cgttgtgatg
      601 cttctttttt cagaccaacc tccataatcc gcctgtaagc ttctaaggct gtctgaaaac
      661 tataaatggc tgtacctgct ttgattgcac tcgtagctaa tacatccaga ctatttgaag
      721 cattttcact gtgattattt ttgctggtgg tcttgttaaa cttagaaagt gtttcacgaa
      781 gagactgatt taactttgct aactcacgat taaaagtttc gtttccaagt gtcttggatt
      841 tactgcttaa gctgtaatac tggcttttaa aatacttctc aagcatagga ttaaagtcac
      901 gaagcttgct catattcgct gagtaagctg ctgtgattgc tctgcttccg ctaattcgtg
      961 gctgtcttgg ttgtctaagt ggctttaaag tatcctcaat ttcagtttta atcttaaacg
     1021 ctctagtgtt tttaagacgc tgctcaatct tggaaacagt tttatcgaca tttctttcta
     1081 ttagtttgag agaatcattt aaaggcgtag gattaactct aaacgactta ttcaatcgct
     1141 tctcaattcg tgatgctacc tgcatcgttt gtttctcggc tttttccaag ccttttaaaa
     1201 ctttctgaga gttaaaactt aactcaatca gaaaatcatc aactttaaat tgtgccattc
     1261 atatttttca cctctttaag cgtgttgtac gtaaattcta tttttcagtg tgggtattag
     1321 ctttacagat ttcacgtagc acacatacaa aaaaagggag agctaccgat gtcactcgat
     1381 aatctctccc cgtatttacc caaccaattc ttttttaatg tcggacaaga taatgtaatt
     1441 ccgtttcacc tatgatttga gcatcagaag gaatacggtc aatacaccac tttggaatct
     1501 tgcgtgtttt tggtttgtgt gaggtgataa cttgacgttt gtttttaatt cttaaggctg
     1561 gcttagtttg taactttaag ttttcacaaa cagaccgaat agtctctaaa cgttcttcaa
     1621 acattaatca cctctttaat tcataatcaa acagccttgc tatacttctg agaaatcttc
     1681 gttaactgct caatatcagc catcaaagca atcttcttct gcgactcata ttcactaaca
     1741 agttctttca tagtatcaag ttctttttta agcttttgct ttaacttatc cagtttaccg
     1801 gaataatgct tagtcaaatt agcttcagct aactgaagaa tctcttcatc tgttttatca
     1861 gtcatcagaa taaattgacc agaacccctt aatgcttgtc tgacatcaat taaccggaac
     1921 ccttcaggga tactttgaag aactttagga acgtcttcaa tcgctgtacc aaaaccatca
     1981 ttaaagtttc cttcataata gataaacggg tttgacttaa tctcgtaagc aatatcatat
     2041 tctgaaggtt tcgaggtttc ttcgaagttc ttaacagcat cgtaagcgac atcaagtcta
     2101 agacgctgtg attttaaagc tgggtaatat tcacgctcaa gctcaaatag tgcttcagtt
     2161 ttgcagatat caggcatttc ttgaggctca ggagacacaa aggaaattac ctttggtgac
     2221 atctttagct cttgctcaag ctcaatgcgc ttgtcatgct cttgacgctg aatagtatgt
     2281 agcggatgtt c
//
LOCUS       DAZOYL010000404         2288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZOYL010000404 DAZOYL010000000
VERSION     DAZOYL010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.408"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHUOY_003972"
     CDS             <1..197
                     /locus_tag="ACHUOY_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0684586.1"
                     /translation="YPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKIIEPVIELYN
                     DVVWASFNTLHNKINPYKES"
     gene            199..564
                     /locus_tag="ACHUOY_003973"
     CDS             199..564
                     /locus_tag="ACHUOY_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0684587.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            561..1226
                     /locus_tag="ACHUOY_003974"
     CDS             561..1226
                     /locus_tag="ACHUOY_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684588.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1226..1591
                     /locus_tag="ACHUOY_003975"
     CDS             1226..1591
                     /locus_tag="ACHUOY_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0684589.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2046..2201
                     /locus_tag="ACHUOY_003976"
     CDS             2046..2201
                     /locus_tag="ACHUOY_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0684590.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          611 a          491 c          618 g          568 t
ORIGIN      
        1 aatatccgcg aaagagtaga acaacagaat tcctgtttct cattctgttt atcgtgttga
       61 tgacaccgat atccccgcta atttttgtct ggataatcgg aaaaataatt gagccagtta
      121 ttgaattgta taacgacgtg gtatgggcgt cattcaacac actgcacaat aaaattaatc
      181 cgtataagga aagctgatat ggcactgacg aaaaaacaac gtgcagaact gcgcatgaag
      241 ttcggtggtc gctgcgctta ttgcggctgc gaacttggcg aaaagtggca tgcagaccat
      301 gtaaaaccgg tcattcgttt tgatggaaat atgcttcacc aggaacgtga cgatatatcc
      361 aacatggtac cagcatgcca cccatgcaat ctgcacaagc attgcagtag cctggaagat
      421 tatcgacgaa ttatcagtga tggtcgtcgt gaattccttg cgtccgggaa aggcaaagcg
      481 ctggttcgta tgggattggt tgaaatgaaa tctgacccgg ttgtgttctg gtttgaaaaa
      541 tatcaagaag gggctacggc atgaccacta ttaccaaaga gcgactgcaa tggctggcta
      601 acatttctgg ccgcgatgat attgacgata tagacggcgg tgaaattcgt gaactggcgc
      661 ttatcgctct ggcatcactg gaagcagagc ctgtagcgga atgcattgtt gaagatgggg
      721 gcatgtgtat tgacgggttc ggtgagtatg tgggtcactc gctgcctgat ggaatgcatg
      781 agctttatgc tgccccgcga atgcgacaac ctgatggatg gaaagccgta gctgtagcgt
      841 ggaaggtgac gtttactcaa gttgaccagg aatctaatac gttcactgct atatattttg
      901 acaaagcgga agttgagtgt tgggtacgac tgcataaagc atgtgatttt cgggcagata
      961 taacaccgct ttacgcagcg ccggcagtgc cggttgcagt aaacgacgac atggcttacg
     1021 cattccatca cgcactgtca gattcatcgc ttggctctga tgaaatcgaa gaaattaaaa
     1081 ccggtttgcg tgctgccttt gccaacgtca ctatccaacc agagccgata gtgccggatg
     1141 aaatcgggcc aaacgatagt aatacgtttg attatgttga tggctggaac gcctgccgcg
     1201 ctgccatgct taagggagat aaataatgat taatcgaacc aaactggagc acattctcga
     1261 atatgccagg cagcagaaat gtattgggca actttgtaaa attccaccag gagatatggt
     1321 tgaaatcgtg gaaatggcca tgcgtaaggc tggcaactct ccggtagctc cggctggctg
     1381 gataagctgt agtgatgcag ttcctgctga atattgcgat gtgattcttc tcgatgatct
     1441 cgggaatgta ttccccggtt cctgggataa ggttttttgc cccactcgtg gcgggaataa
     1501 gatggctttt gtggacaaag acggcgttga agtagagagc tcaactcact ggatgccgct
     1561 accggaacca ccgcaggagg tgaatcaatg acctggcctg aggcattcac cacggtagga
     1621 attgcgatgg cggtggcgct ggtggtgtat tcgatttgcc gttggggtta acaaacaaaa
     1681 accccggatt gatggtccgg ggtttttgaa ggaaacaaaa cagaaacagc aattgccgtt
     1741 acctgttgtt accatggcaa gtaaacgtat ctcaggcgag cgcattgcgc cgttctgacg
     1801 cagataaact agcctggata gatggtgctg gcaataaaaa atagcgtttt cttatcggtg
     1861 tcggtaagat tgctgcgggt gcttgaggct gtctgcctcg ggtatgtcac tgtaaggcag
     1921 acagagaaaa gccccagtta acattacgcg tcctgcaaga cgcttaacat taatctgagg
     1981 cccaatctat gtctcacaaa tgtaggttag cctcttacgt gccgaaaggc aaggagaagc
     2041 aggctatgaa gcagcaaaag gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca
     2101 ttgtgacggc actggtaacg aggaaagacc tctgcgaggt acgaatccga atcggccaga
     2161 cggaggtcgc tgttttcgta gactacgaat ctagagagta agagtgacca ggcgggagag
     2221 taatctcccg ccacctttga tgtgtcaggc atcctcaacg cacccgcact taacccgttt
     2281 cggcggtt
//
LOCUS       DAZOYL010000405         2277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZOYL010000405 DAZOYL010000000
VERSION     DAZOYL010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.409"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHUOY_003977"
     CDS             <1..347
                     /locus_tag="ACHUOY_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0684591.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUOY_003978"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUOY_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR0684592.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUOY_003979"
     CDS             1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUOY_003979"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0684593.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2117..>2277
                     /locus_tag="ACHUOY_003980"
     CDS             2117..>2277
                     /locus_tag="ACHUOY_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR0684594.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGP"
BASE COUNT          549 a          568 c          577 g          583 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctagtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1981 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     2041 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     2101 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     2161 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     2221 tgccattggt acggggttgt ttatggggtc cggcaaaacg attagccttg ccgggcc
//
LOCUS       DAZOYL010000406         2256 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZOYL010000406 DAZOYL010000000
VERSION     DAZOYL010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.410"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(118..1122)
                     /gene="argF"
                     /locus_tag="ACHUOY_003981"
     CDS             complement(118..1122)
                     /gene="argF"
                     /locus_tag="ACHUOY_003981"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR0684595.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1284..1700
                     /gene="rraB"
                     /locus_tag="ACHUOY_003982"
     CDS             1284..1700
                     /gene="rraB"
                     /locus_tag="ACHUOY_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR0684596.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1746..2249)
                     /locus_tag="ACHUOY_003983"
     CDS             complement(1746..2249)
                     /locus_tag="ACHUOY_003983"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0684597.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          574 a          555 c          551 g          576 t
ORIGIN      
        1 ggaagattta cgtatagcaa taaaaaaatt ggggtggaat aattgaacta tcactgcata
       61 aaataatgtg acgccgggat gtttttgtat ttcccggcat ctttatagcg atatcaatta
      121 tttactgagc gtcgcgacca tcaccgcttt gatggtatgc atgcggtttt ccgcctgatc
      181 aaatacaatg ctggcggcag attcgaagac ctcatcagtc acttccattc cgccatgtag
      241 gccaaactct tccgccattt ttttgccaag cgtcgtttgg tcgtcatgaa acgcgggcag
      301 gcagtggagg aatttgacct ccgggttacc ggtcaactgc atcatcttgc tgttcacttg
      361 ataatcacgc agcaatgcaa tgcgttcagc ccatttctct tttgcttcac ccatcgacac
      421 ccatacatcg gtatagataa agtcagcacc ttcaactcct ttagctacat cttccgtcag
      481 cgtaatattc ccgccatttt gttgtgccag ggcgcggcat tccgtaacca gcgcagcttc
      541 cggccagcat gcctgtggcg cgaccagacg caaatccaga ccggtaagcg ctgcagcttc
      601 gagcatcgaa ttgcccatgt tgttacgtgc gtcacctgca tagactagcg tcatttcgtt
      661 gaacgttttg ccgggcaaat gctcctgcat ggtgaggaga tccgccagca gctgcgtggg
      721 gtgaaactca ttggtcaggc cgttccatac cggcacgcca gcgtattccg ccagtgtttc
      781 gacaatctcc tgaccatagc cgcgatactg aataccgtca tacatgcgac caagcacgcg
      841 ggcagtgtct ttaatcgact ctttatgacc aatttggctg ccgcttgggc cgagataagt
      901 aacacgagcc ccctggtcat atgcggcaac ttcgaaagag catcgggtac gggtcgagtc
      961 tttttcgaag atgagcgcga tgtttttacc agtgagtttg gcttcttctt taccgctttt
     1021 cttatcggct ttcagcttcg cggctaactg cagcaggctg ttgagttcag ctggcgtgaa
     1081 atcgagtaat ttcaggaaat gcttatgata aaacccggac ataggtccct cctgtggcta
     1141 acgccttaat gaattaaaat tcaatttata tggatgatta ttcatttgca agtctaaagc
     1201 ataaatcttt atcacaaagg tggaggcaat gtcagtggtg tgtgacaata agagtatcgg
     1261 caggacatta agaggaatga gccatggcaa acccggaaca actggaagaa cagcgtgaag
     1321 aaacacgttt gattattgaa gaattactgg aagatggcag cgatccggac gcgttgtaca
     1381 ccatcgaaca ccatctttcc gcagacgatc tggaaactct ggaaaaagcg gcagttgaag
     1441 cgtttaaact cggttacgaa gtgaccgatc cagaagagct ggaagttgaa gatggtgata
     1501 tcgtgatttg ctgcgacatc ctcagcgagt gcgcgttgaa tgccgatctg atcgatgccc
     1561 aggttgaaca actgatgacg ctggcggaga aatttgacgt tgagtacgac ggttggggca
     1621 cttactttga agatcctaac ggcgaagatg gcgacgatga agattttgtc gatgaagacg
     1681 atgacggggt tcgccactaa ttaattgatt aaaatgcccg atagcacata ctagcgggca
     1741 tttttttaga gttcgcgtag catccgcact tcacaatcga catggcccgt gcagccaagc
     1801 gcatagtcga tatgctcaaa gcccaaatgc tcataaagcg caatggcttc ctttaaaaaa
     1861 gcggtcgttt ccagatagca gcgtttgaag cccatctctc gtgcctgctc catcgccatt
     1921 aaggccagtt ttttcgctag ccctttaccg cgaatagccg gaagaaaata catcttttgc
     1981 agttcgcaaa tgtccgactc actcccggtt aatggcgcaa tcccaccgcc gccgaccact
     2041 tcaccctcgt actcaaccac ccaatatgca tggccaggct gactatacac ttgatacagc
     2101 tcgtcaagat tcggatcggc gacggtgtag cctttatcag cggtaagacc gtattcggcg
     2161 gatacctgac gaatgacgcg ggcgatagca ggattatcca gcagcgtcag gcgacgcatt
     2221 acaggtgatt gcggcgcaat gttattcata gtttaa
//
LOCUS       DAZOYL010000407         2242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZOYL010000407 DAZOYL010000000
VERSION     DAZOYL010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.411"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..298)
                     /locus_tag="ACHUOY_003984"
                     /pseudo
     CDS             complement(1..298)
                     /locus_tag="ACHUOY_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            414..602
                     /locus_tag="ACHUOY_003985"
     CDS             414..602
                     /locus_tag="ACHUOY_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684598.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            612..1811
                     /locus_tag="ACHUOY_003986"
     CDS             612..1811
                     /locus_tag="ACHUOY_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR0684599.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGCLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDIS
                     LLRYMPA"
     gene            2006..2134
                     /locus_tag="ACHUOY_003987"
     CDS             2006..2134
                     /locus_tag="ACHUOY_003987"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684600.1"
                     /translation="MQTQQDTIHQMAEYNRLLSQRMAAYASEINRLKALVAKLQRM"
BASE COUNT          530 a          574 c          653 g          485 t
ORIGIN      
        1 taagcttttt gaatgcctga tcgagcatat tctcgaccat gttcatcact ggtctttccg
       61 aaaggctgta agaaataact tcgttgttga agagatctat tactggagac aaatacagct
      121 tgcgcccatt gactgcaaat tcagtaacat cggtaaccca cttctcgttt ggccgcgtag
      181 ccttgaaatc tctttggaga acattggggg cggtttgccc tacctctcct ctgtaagagc
      241 ggtatcgctt gaccttaatc gctgccttaa gtgagaggat tcccatcagg cgctgaacca
      301 cggcagcctg acggctgcaa attgaaaacc gcccctgttc gtcctgttgt tgccgcacgc
      361 tccgcagaat atgtatgctc tgaggacctg ttttattaag gactcatcct cttatgttga
      421 cccgcaaaag tatagatacc gttctgctct ctgttggtgc tgagaagctc tcacaacgtg
      481 aatgggactg gatgaagatg ctcaaaccca tggacccacc acctgcgatg gtcgcggcct
      541 ccatcctgga gcgccgtggc gacacagcag cccttacccg gctacaggat accgggggtt
      601 aatccgtcct catgttgtcc gcttttactc cccgcccgct gaaacgtctg ttcacggcca
      661 accagtgctg gacgtccttc ctggatgcgg gcggtctgcg cgatatcgag gttgaggctg
      721 tcacaaaaat gctggcctgc ggcacgcgga tactgggagt gaaggaatac aactgcgata
      781 aacctgagtg cccccacgtc agatacgtca ctaactcatg cggcagccgt gcctgcccgt
      841 cctgcggaaa aaaggccaca gacctgtgga tagcgacaca gctgaatcgt cttcctgact
      901 gcgactgggt acatctggtc ttcaccctgc cggacacgct gtggccggtg ttcgaaagca
      961 accggtggct gctgaatgac gtgtgccgtc tggcggtgga gaatctgctg tatgccgccc
     1021 ggaaacgggg gcaggaaccc ggtatcttct gcgccatcca cacgtatggc cgtcgtctca
     1081 actggcatcc gcatgtacat gtgtctgtaa cctgtggatg tctgaataag catggtcagt
     1141 ggaaaaagct gagcttcctg aaagacgcga tgcgttcgcg gtggatgtgg aatatgcggc
     1201 aacggcttct gaaagcgtgg tcagaggggc tggcgatgcc ggagtcgttg tcacatatca
     1261 cgacggaatc acagtggaga agcctggtgc tgaaagccgg aggaaaatac tggcatgtgt
     1321 acatgtcgaa aaaaacggcc ggcgggagga atacggcgcg ctacctgggt cgttatctga
     1381 agaagccgcc gatagcggcc tcccggctgg cacattacaa cggaggggcg agcctgagct
     1441 tccgttacct ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc
     1501 tggtcgcgag gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg
     1561 ggttccttgc caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga
     1621 tggataaacc ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga
     1681 gtcgtgaccc gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccattg
     1741 cggggctgaa tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata
     1801 tgccggcctg acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg
     1861 ttgcggtata agaatcgacc atgcagagag agaaaacacc ggagtggtgg ggaaaaacag
     1921 aaaggctcac gtggcataag gcgcggacag gggtatcgct tagtttttca atttcctata
     1981 cgtgaacgcc cgcctgcggg cactgttgca gacgcaacag gacactatcc accagatggc
     2041 tgaatacaac cgcctgctct cacagcggat ggcggcttat gcttccgaaa tcaaccgact
     2101 gaaggcgctg gttgcgaaac tgcagcgtat gtagttcggt aaaagctcag aaaaacttcg
     2161 cgcaaaaacc gaacggcaga ttccattttc acgggcgatc tacgccacac aggctctggg
     2221 ttgcagtgac tgctccatta ag
//
LOCUS       DAZOYL010000408         2235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZOYL010000408 DAZOYL010000000
VERSION     DAZOYL010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.412"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..259)
                     /locus_tag="ACHUOY_003988"
     CDS             complement(<1..259)
                     /locus_tag="ACHUOY_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFR0684601.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            474..1664
                     /locus_tag="ACHUOY_003989"
                     /pseudo
     CDS             474..1664
                     /locus_tag="ACHUOY_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1849..>2235)
                     /locus_tag="ACHUOY_003990"
     CDS             complement(1849..>2235)
                     /locus_tag="ACHUOY_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0684602.1"
                     /translation="AGQDEIGLRYNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMV
                     RYYGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRF
                     TGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT          578 a          584 c          508 g          565 t
ORIGIN      
        1 aaattttatc cgcgtggtgc atcagcacaa atttatccca caactgttct tctgtctcga
       61 catgcgccgg atctttcaca atagtattgg ggatcgggca caccttctgg caggttggtg
      121 tctcgtagtg ccctacgcat tcggtacact tatcgctgtt aatctcgtag atatgatctc
      181 ccattgaaat cgcctcattc gggcattcgg gttcacacat atcacaatta atacagcgtt
      241 tagtaattag taaagacatt tcaatggatt accgttaaat cattttaaaa tcagtaagtt
      301 gtatcgagtt tgtatgcttt actgtcatta acttactgta tgttgatcca gtgtatttaa
      361 ccttgataaa ctcagtccag caacacaaaa ccgcaacaca ttgcattttg tcccgtagaa
      421 aagacttgta tgtgtgagct tgttttctgc gcctacgcag ataaggattg agaatgccgc
      481 gcactgtaac acataatccg gatagcccca ataatgacga tgttttagcc gcatctgaaa
      541 aatgggacgc ctgtaaaccc ccctatacca gcgcacacat gaaaatctgt gttgctgccg
      601 ccaaaatcat cctcgctgct tccggcgtgg ctcgccgttc caaatacgaa aaagagaact
      661 atctccgtat cgatttcagc aaagccggta aggttacatt ttacgccgag tttccaaaaa
      721 agatgggcct caaaggtaaa aagctcggcg agtggccgga actcgctatc cagctggcgc
      781 gcgaaaaagc actaggtatg gctgacggtg gactgcgggc agagtccgta catgcagcgc
      841 tggaaatgta ccgggatgac ctcaaagcca aagtagcccg gcagaagctg agcccggaca
      901 gtttcacaac ctacggggtg cgtatcgacc ggattaaagc aacgttcggc gagcgtgagg
      961 tgttcagcga cgtaacatac aatcggctgg tggaagtgct ggacgagtgg atcgccactc
     1021 gctcgaacaa taacgccctg gagttgtttg ccgagctccg tcggttctgg aagttctgcg
     1081 cacctactct ttgcaacggc cgcaatgttg ccgccagtct gccagatgat tatgtttcct
     1141 cccgcgtaca gaaacctacc cccacacggc tttttaccga tattgaatca atcgcccgac
     1201 tctggctcaa tgttgctgcc tgcacctctg tacaccagaa gaatgctgtt cgcttcatga
     1261 tcatcactgg tgttcgtccg attaatgtcc ataacctgcg ctgggactac gtttacgagg
     1321 aggctggtga aattgtttat ccggaagggg ttatcggcat gcgaggggct atgaaaacac
     1381 aaaaggcttt ccgcctgccg ataacgcctg agatccggcg gattatcgac gagcagaaag
     1441 cctggcgtga ttcagttcct gagtgcaaca gggattatgt atttttgcag ccacgtgatc
     1501 caatgcagcc attttcaaaa cgatcactgg ataagctggt gaaaacatac agcccggacg
     1561 gggctgtaaa aggaataaaa catgatggga ctgttaaagg gaaagacggt gcatttaata
     1621 cgatgtgccg taaattcctt aagagcaatg ttattgcctt gatcgatagg aattaaaacc
     1681 ccaaaaagat taaaaaaaca ccacaaaacg gatgtttctt caacaccact tttgctcata
     1741 tgaacggaac cgacgattaa actggatggc tctgattgat tcagggtatg aatggcggtt
     1801 ttttgctccg tttccctcaa aatggacgca actttccctc tgcggctctc agccgcacca
     1861 ccgcatccgg gccagcggct catgcatcag gacctgctct gccagacggt agccccgctt
     1921 cagccccgta aaacgcatct gactcccgca cagcacgcac ttcagcgggt caaccttcag
     1981 taacctctga tacatccctc tccaggtgat ttgcatcgcc gtttttctca ctgtctccgt
     2041 tatgatgtac accacttctt ccagtaaccg ccgtttcgcc ggactcaaaa aaccgtagta
     2101 cctcaccata cggaacccct tatccgccac atgccaggag aacctttcca tgaactcatc
     2161 tccactcatc aacaggtatt cttcccgttt tgttcggtga ctgttgtaac gcagaccgat
     2221 ttcatcctga ccggc
//
LOCUS       DAZOYL010000409         2229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZOYL010000409 DAZOYL010000000
VERSION     DAZOYL010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.413"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(11..1057)
                     /gene="aroH"
                     /locus_tag="ACHUOY_003991"
     CDS             complement(11..1057)
                     /gene="aroH"
                     /locus_tag="ACHUOY_003991"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR0684603.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1214..2047)
                     /gene="ppsR"
                     /locus_tag="ACHUOY_003992"
     CDS             complement(1214..2047)
                     /gene="ppsR"
                     /locus_tag="ACHUOY_003992"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR0684604.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT          549 a          539 c          576 g          565 t
ORIGIN      
        1 gggcacgcat tcagaagcgg gtatctaccg cagaggcgag tttttcgacc aggcgttcgg
       61 tatcttccca gcccagacac gggtcggtaa tggattgacc gtaagtgagc ggctgactgc
      121 cgacgatttt ttgcgttcct tcgagcagga aactttccgc cataattcca gcaatcgccg
      181 tagagccatt gcggatttgc tgacaaatat cctcacaaac ttctaactgg cgacggtgct
      241 gcttctggca gttaccgtgg ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt
      301 gcagcgtatc gcaggctgcg gcgatatcat cggcatgata attcggtttt ttgccgccac
      361 gcataataat gtggccatac gggttgccgc tggtctgata gatggtcatc tgaccatttt
      421 tgtctggcga gaggaacata tggctggcgc gggctgcgcg gatagcatcc acagcaatcc
      481 gcgtattgcc atcggtacca tttttaaaac ctaccggaca ggagagtgcc gaagccattt
      541 cacggtggat ctgactttcg gtagtacgtg cgccaatcgc gccccaactg attaaatcag
      601 caataaactg accggtcacc atatcgagga actcagtcgc ggttgggacg cccagctcat
      661 ttacctgtaa aagtaatttg cgcgccagct ccagaccgtg atttacccga tagctgccgt
      721 ttaaatctgg atcggagatt agtcctttcc agccgacaac agttcgtggt ttttcaaaat
      781 aggtgcgcat tacgatttcc agccgtgact ggtactggtt gcgcagcgac tgcagacggg
      841 gggcgtactc cattgcagcg gtgagatcgt ggatcgagca ggggccaata atgaccaaca
      901 gtcgcttatc ttcaccattg agtatttttt caattctgcg gcgggagtcg gtgacatggg
      961 tggcggcgcc aggcgttacg ggataccgta gcgcgagttc ggcgggcgtt accaggctct
     1021 caatacgcgc agtacggagt tcgtcagttc tgttcattac aagtctcaga aatttgttgc
     1081 ttctacggca ggactgccgg gaagtgatgc gcatcacgat aaaccaatcg ctgctgattt
     1141 caagtgcgag gtgtgtcaca cctcgccggg tggttagcat cataacaaaa aaataagcta
     1201 atgcactagt tctctagtac attcggcgac taaggcccat gatatcgagg atcttggtgg
     1261 caatctcttc taccgaataa ttggtactgt taatccacgg gatctgattt ttacggtaca
     1321 acgcttccac ttccgcgact tccatcctgc actgacgaag cgaggcatag cgactgttct
     1381 cccgacgttc ctcgcgaatc gccgccagac gttccgggtc gatagtcagg ccgaacaatt
     1441 tatgctgaag cggtttgagc gacgcgggta gcaccagatt atccatatcg tcggcaataa
     1501 aggggtagtt tgccgcgcgg ataccaaatt gcattgccag ataaagactg gtgggggttt
     1561 taccacagcg agaaacaccg agcaggatca cctgagcctg atccagattg cgcaacgaaa
     1621 tgccgtcatc gtgggcgagg gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga
     1681 gattattagg gttaaggcca tgggtacgat gagcaatcgg cgttggatcc agtttcatct
     1741 cttgttgtag cggggcaacc agcgcctgaa cgatatcctg gcaaaagcct tcactttgca
     1801 agatgatggc gcgaatctcc ggcaacacga tggagtagaa gaccagcggg cgcacacctg
     1861 tctggtgata aattgcgtca atctgatcct tcactgcccg tgcacggctc tcattttcga
     1921 caaacggcag cgtaatgctg ctgatagtga cgggaaattg tgacattact gcgtgtccta
     1981 atacctccgc agttattgcc gtaccatcag aaatataaaa aacgtggcga tcaacagcat
     2041 tatccatttt gttcttcccg tgatgcagac ataattctat gaaagcataa attaaaaacg
     2101 cacagaagcg taggacgtta tgtctggttt ataaaatgaa ccttcaattt tattttttat
     2161 gaaaacagca tttcattttt atggtttcgt ttataccgat ggtttatgtg gaaattgtcg
     2221 aagagagca
//
LOCUS       DAZOYL010000410         2213 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZOYL010000410 DAZOYL010000000
VERSION     DAZOYL010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.414"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(263..463)
                     /locus_tag="ACHUOY_003993"
     CDS             complement(263..463)
                     /locus_tag="ACHUOY_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684605.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="ACHUOY_003994"
     CDS             complement(463..735)
                     /locus_tag="ACHUOY_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR0684606.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="ACHUOY_003995"
     CDS             complement(738..1016)
                     /locus_tag="ACHUOY_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684607.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="ACHUOY_003996"
     CDS             complement(1017..1574)
                     /locus_tag="ACHUOY_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684608.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT          604 a          448 c          422 g          739 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcttg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttacaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccagaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct tatttttcaa taa
//
LOCUS       DAZOYL010000411         2206 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZOYL010000411 DAZOYL010000000
VERSION     DAZOYL010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.415"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(54..263)
                     /gene="fumD"
                     /locus_tag="ACHUOY_003997"
     CDS             complement(54..263)
                     /gene="fumD"
                     /locus_tag="ACHUOY_003997"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR0684609.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            618..812
                     /locus_tag="ACHUOY_003998"
     CDS             618..812
                     /locus_tag="ACHUOY_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684610.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            820..>2206
                     /gene="pykF"
                     /locus_tag="ACHUOY_003999"
     CDS             820..>2206
                     /gene="pykF"
                     /locus_tag="ACHUOY_003999"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR0684611.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTT"
BASE COUNT          608 a          550 c          487 g          561 t
ORIGIN      
        1 ccgcaactga agcaagaata tcaggatgaa gcttaccgcc tcatcctgaa tggtcatttt
       61 accagtgcgt taatgacggt ttccatcgcc tgcttttcca gctcactgcg ctgaatgcgt
      121 ttattggcta atgcagtacg taatacgcct gcgataacaa tatgctttgg ttcctgcccc
      181 agatcgcgca tttccagcac aactttaccg acaactctgc acatttctcg gtacagttca
      241 tcctcttttg tccgatttcc cattctttat acctatttat catgccaact atcagcatat
      301 atcaatctaa ccaattaaac aaaaatcaaa caaaatcaga caaataacgc gataaattat
      361 tttaactgta gcaattgagc gatgatatat ttatacaccg gatgaacttt cacttatcct
      421 cacactgaca acttcggcac cagacgttgc gcaaacagcg aagtttttgc gtaacctttt
      481 ccctggaacg ttaaatcttt gataacaatt tattgtctaa caagttgtat attttttgaa
      541 acgctgtttt tgttttcctt ttggattaat ttcagcgtat aatgcgcgcc aattgactct
      601 tgaatggttt cagcactttg gactgtagaa ctcaacgact caaaaacagg cactcacgtt
      661 gggctgagac acaagcacac attcctctgc acgctttttc gatgtcacct atccttagag
      721 caaggcacca ccactttcgt aataccggat tcgctttccg gcagtgcgcc cagaaagcaa
      781 gtttctccca tccttctcaa cttaaagact aagactgtca tgaaaaagac caaaattgtt
      841 tgcaccatcg gaccgaaaac cgaatctgaa gagatgttag ctaaaatgct ggacgctggc
      901 atgaacgtta tgcgtctgaa cttctctcat ggtgactatg cagaacacgg tcagcgcatt
      961 cagaatctgc gcaacgtgat gagcaaaact ggtaaaaccg ccgctatcct gcttgatacc
     1021 aaaggtccgg aaatccgcac catgaaactg gaaggcggta acgacgtttc tctgaaagcg
     1081 ggtcagacct ttactttcac cactgataaa tctgttatcg gcaacagcga aatggttgcg
     1141 gtaacgtatg aaggtttcac tactgacctg tctgttggca acaccgtact ggttgacgat
     1201 ggtctgatcg gtatggaagt tactgccatt gaaggtaaca aagttatctg taaagtgctg
     1261 aacaacggcg acctgggcga aaacaaaggt gtgaacctgc ctggcgtttc cattgccctg
     1321 ccagcactgg ctgaaaaaga caaacaggac ctgatctttg gttgcgaaca aggcgtagac
     1381 tttgttgctg cttcctttat tcgtaagcgt tctgacgtta tcgaaatccg tgagcacctg
     1441 aaagcgcacg gcggcgaaaa catccacatc atctccaaaa tcgaaaacca ggaaggcctc
     1501 aacaacttcg acgaaatcct cgaagcctct gacggcatca tggttgcgcg tggcgacctg
     1561 ggtgtagaaa ttccggtaga agaagttatc ttcgcccaga agatgatgat cgaaaaatgt
     1621 atccgtgcac gtaaagtcgt tatcactgcg acccagatgc tggattccat gatcaaaaac
     1681 ccacgcccga ctcgcgcaga agccggtgac gttgcaaacg ccatcctcga cggtactgac
     1741 gcagtgatgc tgtctggtga atccgcaaaa ggtaaatacc cgctggaagc ggtttctatc
     1801 atggcgacca tctgcgaacg taccgaccgc gtgatgaaca gccgtctcga gttcaacaat
     1861 gacaaccgta aactgcgcat taccgaagcg gtatgccgtg gtgccgttga aactgctgaa
     1921 aaactggatg ctccgctgat cgtggttgct acccagggcg gtaaatctgc tcgcgcagta
     1981 cgtaaatact tcccggatgc caccatactg gcactgacca ccaacgaaaa aacggctcat
     2041 cagttggtac tgagcaaagg cgttgtgccg cagctggtta aagagatcac ttctactgat
     2101 gatttctacc gtctgggtaa agaactggct ctgcagagcg gtctggcaca caaaggtgac
     2161 gtcgtagtta tggtttctgg tgcactggta ccgagcggca ctacta
//
LOCUS       DAZOYL010000412         2200 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZOYL010000412 DAZOYL010000000
VERSION     DAZOYL010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.416"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..595)
                     /locus_tag="ACHUOY_004000"
     CDS             complement(<1..595)
                     /locus_tag="ACHUOY_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684612.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
     gene            complement(1025..1348)
                     /locus_tag="ACHUOY_004001"
     CDS             complement(1025..1348)
                     /locus_tag="ACHUOY_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684613.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            complement(1516..1935)
                     /locus_tag="ACHUOY_004002"
     CDS             complement(1516..1935)
                     /locus_tag="ACHUOY_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684614.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            complement(1935..>2200)
                     /locus_tag="ACHUOY_004003"
     CDS             complement(1935..>2200)
                     /locus_tag="ACHUOY_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684615.1"
                     /translation="PEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTPFDAIKGVAE
                     GSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
BASE COUNT          628 a          467 c          406 g          699 t
ORIGIN      
        1 tcagcttagc cagtcttgat ttcagcaaat taatctcatt ttctcttgct ttcatcagtt
       61 tctctttggc aatttcttca agctcagctt gtgatttatc agcaacaatt agagacagtc
      121 gatgtggtgc tctgacagct ctttctgtac gaacaactct ttgaccttct ggtaaatctg
      181 caatcacttt accaatctct acagggtcat acccccaacc gtcacggtct agtcgagaga
      241 aataagcata agaattggtt tgaagctcca tcgaaataac catttcgtca atttcgtctt
      301 caagcttgga ctgaattttc tcgtaaacac ttctcattgc ttcttgagag tctttaatag
      361 cttggaaata tcggcgttgt agcttcaact tcaaatcaag ttcacaccgt tcgttcatag
      421 aaaaatcaat ttcaactctt ggaatctcga gtggttctgg tggaatatat ggacctttat
      481 ttacttcgtg tgattcccgc caagctctat caaaagattc acgcaaagtt aattcgtgaa
      541 tatcaagtgc acttggtctg tgttcgataa gcatttcatg ttcatttcta atcattcgtt
      601 caccccttag tttatttgtt atttcaattt atagaagcac actcagcaat cagaggtgaa
      661 gagtctgact gaacaagaac ctgccaagtg tgcttttaaa aactgaaaaa gccctaaaca
      721 tttctgtgaa gggcaaagga ttcaataatg aatgaacaag agtacttttt gagatagcct
      781 ctcagactaa cctgaaaggc catgacgaaa agcattgcac ctttccaact cagaatatcc
      841 atccatcgga ttattgaaag aaaaataaga gagaattgct ctcccataat taaaattatg
      901 ccatgcttaa ggttatttgt caacagcttt ttaacaggtt gataacatca attcttattt
      961 accgcattgt taataataca accaacaaat aagccaagca acgtccaagc aattattaaa
     1021 cccattagtg aaccctcaca gaatccgtca ccagaatatt atcactgtac aggattgtca
     1081 cagcaccttt caaaaattgc tctggggaaa tactgtatat gttcgtttgc tcgataacca
     1141 atcttttcat gttgctgtct gtaattggta gcatcattcg aggctcgtta aactccaccg
     1201 actgaataaa gtcagagatg ttttctacat tatcagccat cagctttagt aatttatcaa
     1261 aattaattgc gatacctgca tcaatgaagc tttgcaccat catagcagtg tcgttatcaa
     1321 ccataatttg cattgggtaa ttattcattt ttaaatctcc tatgaaacac acctcagacc
     1381 tcttaaaata gccattctga ggccagtaac catgcgggtt acagagggag ttgcactggc
     1441 tggtaaggta atttatcaaa ttaaggtgca actctgcctg taaagtgcat tctaccgttt
     1501 cttagatggt tatttttaat tgtctttatt aatcaatggc ttaatgtaga aatactgtct
     1561 ccgtgaaata ccatattttt ctgtaatgta tcgctgagat tgaccagcat ttatggcctg
     1621 agtgatatca tcacggttat agcggttacc acgggattta tatttgtcac catccacatc
     1681 ttctgaggta ttgaagtctt caatcagctt aattgcatcg tgaaggtcat cttctgaggc
     1741 ataatcgaag gtatggataa tctcaaaatc accagtatct gctggtgcat tcttccatct
     1801 ggagaagcgg gattttaatt gctggtgtgt gaagggttca gcattacgag ttaaatcttt
     1861 ccatgttccg ttattctcac ggacaaggtg acctctatgg cgataaaacg catgataatc
     1921 aaatgtgcat agcattaaac ccccagctta tccgccagtt gatgaacctt ggcactcaac
     1981 acctgaatca ttgagcaacc tgccactgcg gactgtgctt tgagtagttc cagttggccc
     2041 gttgtgattc tgatggcctt agaaccctct gcaacgccct tgatagcgtc aaacggtgta
     2101 agggctatgt gttccttcaa aatctcacca acaagtgctg tgtggctcat ggctgggtat
     2161 ttgtctttga ttgctgccag tctggaatat gcctctggca
//
LOCUS       DAZOYL010000413         2179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZOYL010000413 DAZOYL010000000
VERSION     DAZOYL010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.417"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            149..658
                     /locus_tag="ACHUOY_004004"
     CDS             149..658
                     /locus_tag="ACHUOY_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR0684616.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            655..2073
                     /gene="phrB"
                     /locus_tag="ACHUOY_004005"
     CDS             655..2073
                     /gene="phrB"
                     /locus_tag="ACHUOY_004005"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR0684617.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
BASE COUNT          530 a          537 c          581 g          531 t
ORIGIN      
        1 atgcagctcg ggaaatagta gaataattct cactcagatg aagttgataa tatttttacc
       61 aacaaaatac ttccccgcaa aaactctgcc ccataaaatt catttaccat cgtctacact
      121 tactccttta taccgttcag gagcagtaat gaatctacaa cgatttgatg acagcaccct
      181 aatccgtatc tttgcccttc atgagttaca tcgactgaaa gaacatggct taacgcgcgg
      241 ggcgcttctc gattatcaca gccgctataa actcgtcttt ctggcgcatt ctcagccgga
      301 gtaccgcaaa cttggcccgt tcgtggctga tattcaccag tggcaaaatc tggatgacta
      361 ttacaaccag taccgccaac gcgtagttgt tttgctttct caccccgcca acccgcgcga
      421 tcacaccaat gttttgatgc acgttcaggg ttattttcgc ccgcatattg attccacaga
      481 acgccagcag ctggctgcgc ttatcgacag ttatcgccgt ggcgagcaac cacttcttgc
      541 gccgctgatg cgtatcaaac actatatggc gctttatcat gacgcctggc tttcagggca
      601 gcgttatttc gaactttggc cgcgtgtgat taacttgcgc cattcaggag ttttatgact
      661 acccatctgg tctggtttcg ccaagattta cgtctgcacg ataatctcgc actggctgcc
      721 gcctgccgca attcgtctgc acgcgtgctg gcgttgtata tcgctacacc acgccagtgg
      781 gcgacgcata acatgtcgcc gcgtcaggct gaacttatca atgctcaact gaatgggcta
      841 caaatagcgc ttgcggaaaa aggtattcct ttattgttcc gtgaagtgga tgactttgtc
      901 gccagtgtcg aaatagttaa acaggtgtgc gcggaaaaca gcgttaccca cctgttttat
      961 aactatcagt atgaagtgaa tgagcgggcg cgggatgtgg aagttgaaag agcgctgcgt
     1021 aacgtggtgt gtgaaggatt tgatgacagc gtgatcctgc cgcctggcgc ggtgatgacc
     1081 ggtaatcacg agatgtacaa agtctttacg ccttttaaga atgcctggct gaaacggctg
     1141 cgggaaggga tgccggagtg cgtcgctgcg ccaaaagttc gtagtagcgg atcgatagag
     1201 ccctcgccat ccattacgct gaattatcct cgtcagtctt tcgatactgc gcattttccg
     1261 gtggaagaaa aagcggcgat tgcgcaatta cgccagtttt gccagaacgg tgccggagaa
     1321 tatgagcaac aacgagattt tccggcagtg gaaggcacca gccgtttgtc ggccagcctg
     1381 gcaacgggcg ggttatcgcc tcgccagtgc ttgcatcgct tgttggctga acagccgcag
     1441 gcgctggacg gtggggtcgg tagtgtctgg cttaatgagc tgatctggcg cgagttttac
     1501 cgtcacctga taacgtatca cccctcgttg tgtaaacatc gtccaattat tgcctggacg
     1561 gatcgtgtac agtggcagag caatcccgca catttacagg cctggcagga aggcaaaacg
     1621 ggatacccga ttgttgatgc cgctatgcgt cagcttaaca gcactggctg gatgcataac
     1681 aggctacgga tgattacagc cagttttctg gtgaaagatt tattgatcga ctggcgcgaa
     1741 ggcgagcgat atttcatgtc gcagctgatt gatggtgatt tggcagccaa taacggtggc
     1801 tggcagtggg ccgcttcaac cggaaccgat gcagcgccgt attttcgtat tttcaacccg
     1861 acaacccagg gcgagaaatt tgatctcgaa ggtgagttta tccgccagtg gctaccggaa
     1921 ctacgcaatg taccggggaa atcggtgcat gagccgtgga agtgggcgca gaaagcaggt
     1981 gtgaagctgg attatccgca accgatagtc gagcacaaag aggcgagagt acaaacgttg
     2041 gcagcgtatg aggcggcgcg gaaggggaaa taacagacgc gtcaggcaat cgagcccaga
     2101 tgccggatgc ggcgtgaacg ccttatccag cctacaaatt tgtgcaaatt caataaattg
     2161 caggaaatac gtaggcctg
//
LOCUS       DAZOYL010000414         2167 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZOYL010000414 DAZOYL010000000
VERSION     DAZOYL010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.418"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            167..2152
                     /gene="yjcS"
                     /locus_tag="ACHUOY_004006"
     CDS             167..2152
                     /gene="yjcS"
                     /locus_tag="ACHUOY_004006"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR0684618.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYHGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          578 a          572 c          526 g          491 t
ORIGIN      
        1 tcgcgtaatt ttccctcttc agcacaaatg cctaataaaa tggatcgttc agctcacact
       61 tccacttatt aaagtcgcaa attcccaatt atgataaccc cagctttaaa tgacgtattt
      121 tgcatggatt acatatttac gacataaatg ataaatggag atttacatga ataactctcg
      181 gttattccgt ttgagcagaa ttgttattgc gttaactgcc gccagcggca tgatggtaaa
      241 taccgctaac gcgaaagagg aagcgaaagc cgccactcaa tatacccaac aggttaatca
      301 gaattacgcc aaatcattac cgtttagcga tcgtcaggat tttgacgacg ctcagcgtgg
      361 atttatcgct ccgctgctgg atgaaggtat tctgcgcgat gctaatggca aaccatatta
      421 tcacggggaa gattataaat ttgatatcaa tgcgcccgcg cccgaaaccg ttaaccctag
      481 cctgtggcgt cagtcgcagt taaacggtat ttccggcctg tttaaagtca ccgacagaat
      541 gtaccaggtt cgcggtcagg atatctccaa catcaccttt atcgaaggtg ataccgggat
      601 tattgtcatc gacccgctgg tgacgccacc gagcgcaaaa gccgcccttg acctttactt
      661 ccaaaatcgc ccgcaaaaac cgattgttgc ggttatttat acccacagcc ataccgacca
      721 ctatggcggc gtgaaaggca ttatctctga agctgatgtg aaatccggta aagtgcaggt
      781 tatcgcccct gctggcttta tggacgaagc catcagcgaa aacgtactgg cgggtaatat
      841 tatgagccgc cgtgcacttt actcctacgg cctgctgctg gcgcataacc ctcagggtaa
      901 catcggcaat ggtcttggcg taacgctggc atcgggctac ccgagcatca tcgcaccgaa
      961 caaaaccatc accaaaaccg gtgagaagat gattatcgac ggcctggagt ttgacttcct
     1021 gatgacccca ggtagcgaag caccagccga aatgcacttc tatattccgg ccctgaaagc
     1081 gctgtgtacc gccgagaacg ccacgcatac cctgcacaac ttctacactc tgcgcggcgc
     1141 gaaaacccgc gacaccagca agtggaccga gtatctgaac gaaacgctgg atatgtgggg
     1201 taacgacgcg gaagtcctgt ttatgccgca cacctggccg gtctggggca ataagcatat
     1261 caatgattat attggtaaat atcgcgatac tatcaagtac attcacgacc agaccctgca
     1321 cctggcgaac cagggctaca ccatgaatga aatcggcgac atgattaaac tgccgcctgc
     1381 acttgccaat aactgggcca gccgtggcta ttacggttct gtcagccaca acgcccgcgc
     1441 ggtgtataac ttctatcttg gctattacga cggtaacccg gctaacctgc atccgtatgg
     1501 tcaggtggag atgggtaaac gttacgtgca ggcgctgggc ggttctgccc gtgtcatcaa
     1561 cctggcgcaa gaagcgaaca agcaaggtga ttaccgctgg tcggcagaac tgctgaaaca
     1621 ggtgattgcc gccaacccgg gtgaccaggt cgcgaagaat ctgcaagcga ataactttga
     1681 acagctgggc tatcaggccg agtccgccac ctggcgcggt ttctacctga ccggcgcgaa
     1741 agagctgcgc gaaggggtgc ataagttcag ccacggcacc atcggttccc cggacaccat
     1801 tcgcgggatg tcggtcgaaa tgctgttcga ctttatgtcc gttcgcctcg atagcgcgaa
     1861 agccgcgggt aaaaatatca gcctgaactt caatatgagc aatggcgata acctcaacct
     1921 gacgctgaac gatagcgtgc ttaactaccg taaaacactg caatcccaag ctgacgcctc
     1981 tttctacatc agccgtgaag atctgcacgc cgtgctgacc gggcaagcca aaatggcgga
     2041 tctggtaaaa gcgaagaaag ccaaaattat tggcaatggc gcgaaactgg aagaaattat
     2101 cgcctgtctg gataatttcg atttgtgggt gaatatcgta accccaaatt aatgtcactc
     2161 gcttttg
//
LOCUS       DAZOYL010000415         2144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZOYL010000415 DAZOYL010000000
VERSION     DAZOYL010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.419"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..480
                     /locus_tag="ACHUOY_004007"
     CDS             <1..480
                     /locus_tag="ACHUOY_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFR0684619.1"
                     /translation="FALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVID
                     IKFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADE
                     SIQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            517..2144
                     /locus_tag="ACHUOY_004008"
                     /pseudo
     CDS             517..2144
                     /locus_tag="ACHUOY_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          674 a          402 c          419 g          649 t
ORIGIN      
        1 tttgctctca gtttccgtta ccccggctgg gcaaaaatga tgattattaa tggtgttact
       61 tttactaaaa atgatactca aaactcactt attattttag atagaacatg gcaacatcat
      121 gatgtgattg acattaaatt tatagctgat atacagttta atactgactt atgtggtgac
      181 acttatattt ccagaggtcc cgttttatat gcaattgagc ttgagagcga tattttaatc
      241 aaaaaaaacc tgctgaacgg gaaatatttt gactcaggat acataccctg tagtagagct
      301 gatgaatcta tccagttctc ttatgctgat atggaatctt tttcatattc aacctcacca
      361 gagggcaaac aatacattga aggctgtttt tatttaaaca agactaaaat agtcagaaaa
      421 cttataccat ttggtcaaac cattcttcgc aaagtaactt tctccaatat agagagttaa
      481 tattttcgag tgttataatt aattaaaggg atttctatgt accttactga taatattatc
      541 aaactgtcga ataaaatagg tcagcgtgtt tatactgttg tctctgaaat gtcgattgaa
      601 gcatggataa caaaagaacc gcagcctttt gtttatcgcc gtagcggcgc ttaccagaaa
      661 ctcaccattg gctcagactg gggtcaactg tttgactgtg cctggatgcg agtttatggt
      721 aaacgtccag cagataaatt tagacacaaa ctcgtcgctc tcgttgatat cggtggcgaa
      781 ggtcttattg tggataagaa tggttcaccc atctgcggca tcacgaataa agcatcatct
      841 tatggcgtac cgccagataa accaggtaaa tgggtggttg acctttctct cgttagtgaa
      901 aatgacgagg ttgagttttg gattgatgct gcctgcaacg atttatttgg ctatgttact
      961 aatggaggga ttattactga tgttcatatc gcgacacgta atcaactact gaaatccctt
     1021 tattatgata ttgaggtgct tttcgactgg attaatgatg gtcagaagtt tgaatccata
     1081 catcctaaag gtatcacccc agaacaattt attgccaaaa gaagtgagcg tactgacgaa
     1141 atcataagta ctctcgaata tattgataat acgttaatta cctttagcaa tgaagaaata
     1201 attaaatgtc aaattgcaat ccaatccata atcaataaaa acaataatcc ttcagagttc
     1261 aggattatgg ctactggtca tgcccattta gatatagcgt ggatgtggcc tttaagagaa
     1321 ggacgcagaa aagcaataag aacatttgcc accgcccttg caaacattga aaaatatcct
     1381 gattacattt ttggcgctag tcagtaccaa ctttttcact ggataaaaaa agattatccc
     1441 tatttcttcg aaaagttaaa aaaacaaatc gccgcaggcc gctttgagtt acaaggttgc
     1501 ttctgggtcg aatgcgattt aaatctggta agtggtgaat cgctgataag gcaaatcatg
     1561 catgggacac gattcacact gcagaatttt tcaaaaaaga taaattatgt ctggcaaccg
     1621 gatgtctttg gattccccgc aacgcttcca caaattctca aaaaatcagg cattaactat
     1681 attgcatcgc aaaagctctc acaaaacaag attaataaat ttaataatta ccttttccgc
     1741 tggcagggcc tggatggctc agaaattctg atgcataact ttcctgaaga cacttatgat
     1801 agccgagcac gtgctcgctc tttagaatat attgaacaaa actataatga aaaagagatc
     1861 tgtccctatg cgcttatggt gtatggtgta tggtgtcggt gatggcggcg ctggccccgg
     1921 tgaggaacat atagaacgat taacacgtat acgcaatatt gatggcttac ctcatgttga
     1981 tttcagccgt gttgataagt tcttcactta cgccgacgcc tttcgcgaaa gcttgcccat
     2041 tatttcaggt gaactttatt tcgaggctca tcaagggtgc tttacatcag agtcagcaac
     2101 taaagctcat aatcgaaata tggaaaataa attacatgat gctg
//
LOCUS       DAZOYL010000416         2130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZOYL010000416 DAZOYL010000000
VERSION     DAZOYL010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.420"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="ACHUOY_004009"
     CDS             complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="ACHUOY_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR0684620.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
BASE COUNT          525 a          487 c          559 g          559 t
ORIGIN      
        1 aactgaggaa tgatattttt caccacgccg ccagcattgg cttcgcgttc aaacagcgta
       61 accggatggc ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca
      121 atcacggcaa ccggatggcg tgaaccagaa ccggcgggtt tgtgccagcg ttgcttatat
      181 tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg
      241 taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggca
      301 ggtagggcgt tgcgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc
      361 agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg
      421 tagcaatcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt
      481 ttctgggtgt attccatggt taacgcgtct gctgccagtc tgttcagtcg ttcgacgtca
      541 acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc
      601 agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat acctgtatca
      661 aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg
      721 tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct
      781 gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta
      841 gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtaccatcag gcgctccagc
      901 atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg
      961 taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggtcggg
     1021 ttaagtttga caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg
     1081 atttcatgcg gaggacagcc gtgcatggtg gagaggggga cgccctgcac catactggtg
     1141 gggatgcgag cgggtaaggc ttgcaagttt tcgcgttttt cctgcaatcc gtggcgggct
     1201 aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc gaatttcgga
     1261 tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct
     1321 tcgaggttgt aaccgatgct catattaaag atgaacgatt taccggaatc agaaggctgt
     1381 aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta
     1441 agcaaggtaa attcggtaga ccattcggtg ttaaagcatt cgtcttcggc atcaatacag
     1501 ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg
     1561 ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg
     1621 ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag
     1681 taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg
     1741 cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta
     1801 gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga
     1861 gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggtggct
     1921 cgaaacttaa aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact
     1981 cctgaaaatt attattgtca gcccatttat cactttttct cttttctcaa aatcagtgat
     2041 tccttatcat tatgataaag agtgctggag agattaaata tttgaacgca atcgtttaac
     2101 tacattttct aaattttagt ataccttttt
//
LOCUS       DAZOYL010000417         2101 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZOYL010000417 DAZOYL010000000
VERSION     DAZOYL010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.421"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..326
                     /locus_tag="ACHUOY_004010"
     CDS             <1..326
                     /locus_tag="ACHUOY_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0684621.1"
                     /translation="VITMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGAS
                     YRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMK
                     DSQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="ACHUOY_004011"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="ACHUOY_004011"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR0684622.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          522 a          512 c          487 g          580 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta tggctttaga
     2101 a
//
LOCUS       DAZOYL010000418         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZOYL010000418 DAZOYL010000000
VERSION     DAZOYL010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.422"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHUOY_004012"
     CDS             complement(60..1025)
                     /locus_tag="ACHUOY_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR0684623.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHUOY_004013"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHUOY_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684624.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          437 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga gcccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZOYL010000419         2088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZOYL010000419 DAZOYL010000000
VERSION     DAZOYL010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.423"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHUOY_004014"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHUOY_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR0684625.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="ACHUOY_004015"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="ACHUOY_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR0684626.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAZOYL010000420         2073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZOYL010000420 DAZOYL010000000
VERSION     DAZOYL010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.424"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            2..698
                     /locus_tag="ACHUOY_004016"
                     /pseudo
     CDS             2..698
                     /locus_tag="ACHUOY_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            705..1451
                     /locus_tag="ACHUOY_004017"
     CDS             705..1451
                     /locus_tag="ACHUOY_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0684627.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1466..1888
                     /locus_tag="ACHUOY_004018"
     CDS             1466..1888
                     /locus_tag="ACHUOY_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0684628.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1889..>2073
                     /locus_tag="ACHUOY_004019"
     CDS             1889..>2073
                     /locus_tag="ACHUOY_004019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684629.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT          568 a          456 c          604 g          445 t
ORIGIN      
        1 tattcgcttc tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc
       61 tcgcggcgtt acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc
      121 gttaattcag gttggatggc tggtcgaaaa tgacggaggg ctttctctac ctaactttga
      181 acgtcataac ggaaaaagct ctaaaaaaaa cgggcggtta caaacgagcg agtaacaaaa
      241 atacgcgaac tgaaacgaaa aggtaacgct gccagcgtta cacaaacgga tcaaaaagcg
      301 ttaccagagg aagaggaaga ggaagaggaa gaggaagagg aagaggaaga tctaaatact
      361 gatctccccc tcgccaaaaa cgagcgtcta aaaaattcga gccggaggct attgagctgc
      421 ccgattggtt gccggaaaca ctctggcatg agtgggtccg gttcagacag gcattgcgaa
      481 aaccgattcg aacggagcag ggcgctaacg gggcgatacg ggaactggaa aaattccgtc
      541 agcagggttt tacacctgag caggtgattc gacacagcat cgccaatgaa taccagggcc
      601 tgttcgcgcc gaaaggtgtt cggcctgaga cgttgctccg acaggttaac accgtctcgt
      661 tgccggacag tgcgatcccg ccaggcttca gggggtaacg gaccatgaaa aatattgcga
      721 caggcggcgt tctggaacgc atccgccgac tgaccccgcc acatgtaacc gccccattca
      781 gaacggtagc ggagtggcgc gagtggcaac ttgctgaagg ccagaaacgt agcgaggaga
      841 tcaaccgcct gaatcgccag ttgcgggtgg aaaaaattct gaatcgctca ggcatccagc
      901 cgttgcaccg taaatgctcg tttgcgaatt accaggtgca gaacgacggc cagcgatacg
      961 cgttaagcca ggcgaaatcc atcgccgatg aactgatgac cgggtgtaca aattttgcgt
     1021 tcagcggaaa acctggtacc gggaagaatc acttagcggc agctatcggg aatcgcctgc
     1081 tgaaagacgg tcagacagtg attgtggtta ccgtggctga tgttatgagc gccctgcacg
     1141 ccagctatga cgacgggcag tcaggcgaaa aatttttgcg ggagctgtgc gaagtggatc
     1201 tgctggttct tgatgaaatt ggcattcagc gcgagacgaa aaacgagcag gtggtgctgc
     1261 accagattgt tgatcgccgg acagcgtcga tgcgcagcgt ggggatgctg acaaacctga
     1321 actatgaggt gatgaaaaca ttgctcggcg agcgggtgat ggatcgcatg gtcatgaacg
     1381 gcgggcgctg ggtgaatttt aactgggaga gctggcgtcc gaatgttagc cattcgaggg
     1441 ttgttaagta gtttcaggag gatttatggc gaaacctttt actcccgaac agcgggaaga
     1501 actgaagacg cgaattgtgg aactcgtgca tcaggacggt cgggtcacga ttcggcagtt
     1561 gtcagatgaa acaggtatca gtcgtgcgtc tgtcggtcgc ttatgcatag aactggtcgc
     1621 aagtggtgat gtatataatt ctggctacgg cttattcccg tctgaacagg ctcgcaagga
     1681 ctggcaaagc gcccgcaaaa aactctcgag agtaaaggtg aggaaaccgg ttgttgttga
     1741 tccggacctt atctggtcat tacctgacgg agaaatacgc cgctacgaca ggcgcctgaa
     1801 tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
     1861 gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc aattaccgct
     1921 ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt taagcaatat
     1981 cgttttattt ccggtaaaag aggatgaccc tcgtaatcag gttaattttc tttatgagcc
     2041 atcggaaaga ccatattgcc atcacgcctc tgt
//
LOCUS       DAZOYL010000421         2048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZOYL010000421 DAZOYL010000000
VERSION     DAZOYL010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.425"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHUOY_004020"
     CDS             <1..389
                     /locus_tag="ACHUOY_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684630.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUOY_004021"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUOY_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR0684631.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUOY_004022"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUOY_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR0684632.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHUOY_004023"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHUOY_004023"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHUOY_004024"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHUOY_004024"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          536 a          496 c          497 g          519 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggcttactgc ttaatttgat gcctggcagt tccctactct
     2041 cgcatggg
//
LOCUS       DAZOYL010000422         2035 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZOYL010000422 DAZOYL010000000
VERSION     DAZOYL010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.426"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            111..1244
                     /gene="ompN"
                     /locus_tag="ACHUOY_004025"
     CDS             111..1244
                     /gene="ompN"
                     /locus_tag="ACHUOY_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR0684633.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1385..1819
                     /gene="uspF"
                     /locus_tag="ACHUOY_004026"
     CDS             1385..1819
                     /gene="uspF"
                     /locus_tag="ACHUOY_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR0684634.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT          562 a          465 c          457 g          551 t
ORIGIN      
        1 ttaaggcgta acaaatgata tttatcggct aactgaactt ctcctttagg atgttttcac
       61 tccccggaat ggggataaaa ggcaaataaa ataactaaag gatttattca atgaaaagca
      121 aagtactggc acttttaatt cctgccctgc tcgccgcagg tgctgcacat gcagccgaag
      181 tttataataa agacggcaac aaattagatc tgtatggcaa agttgatggc ctgcattatt
      241 tttctgataa ttcagcgaaa gatggcgatc agagctatgc gcgtctgggt tttaaaggcg
      301 aaacccaaat taacgatcaa ctcactggct acggtcaatg ggaatacaat attcaggcaa
      361 acaacactga atcttcaaaa aaccagtcat ggacccgtct ggcatttgcc gggctgaaat
      421 ttgcagatta cggttctttc gattacggac gtaattatgg cgtaatgtac gacatcgaag
      481 gctggaccga tatgctgcct gaatttggcg gtgactctta taccaatgca gacaacttta
      541 tgactggtcg tgccaacggc gtcgcgactt atcgtaatac tgatttcttc ggtctggtaa
      601 atggtctgaa cttcgcggtg cagtatcaag gtaacaacga aggtgccagt aatggtcagg
      661 aaggcaccaa caacggacgt gatgttcgcc atgaaaatgg tgacggctgg ggtctttcca
      721 caacatatga tttaggcatg ggctttagcg ctggtgcggc atacacctca tctgaccgca
      781 ccaatgacca ggttaaccat actgcggcgg gtggtgataa agcagacgcg tggactgctg
      841 ggctaaaata cgatgctaac aatatttacc tggcaaccat gtattcagaa acgcgtaata
      901 tgaccccgtt tggcgacagc gattatgctg ttgcaaacaa aacccagaac tttgaagtca
      961 ctgcacagta ccagtttgat tttggtctgc gtccggcagt ctctttcctg atgtctaaag
     1021 gccgtgacct gcacgctgcc gatggtgcag acaacccggc aggtgttgat gataaagatc
     1081 ttgttaaata cgccgatgtt ggcgcgactt actatttcaa taaaaacatg tccacttatg
     1141 ttgactataa aatcaacctg ttggatgaag atgacagttt ctacgctgcc aacggcatct
     1201 ctaccgatga tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg
     1261 ttatggcggg ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc
     1321 cgtcattaca caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg
     1381 ttttatgaac agaacgattc ttgtccctat cgatatttcc gattcagaat taactcaacg
     1441 cgtgattagc cacgttgagg cagaggcaaa gattgacgat gcagaggttc atttcctgac
     1501 ggtaatacct tcactgcctt actatgcctc tctgggttta gcgtattccg cagaattacc
     1561 ggcaatggat gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt
     1621 taaactgcca accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat
     1681 tctggaattg gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga
     1741 tatcaccact tatctgctcg gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc
     1801 cgtgctggtt gtgcgctgac actaacgccc gcacattgct gcgggctttt tgattcattt
     1861 cgcaaatgtg ctgacatttt cccctctaat ccgtaccata cacgccacag ttttatatca
     1921 gatttcttat gctgggcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt
     1981 gagtcgcttt taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaac
//
LOCUS       DAZOYL010000423         2024 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZOYL010000423 DAZOYL010000000
VERSION     DAZOYL010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.427"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1564)
                     /locus_tag="ACHUOY_004027"
     CDS             complement(<1..1564)
                     /locus_tag="ACHUOY_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR0684635.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVV"
     gene            complement(1779..>2024)
                     /locus_tag="ACHUOY_004028"
     CDS             complement(1779..>2024)
                     /locus_tag="ACHUOY_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684636.1"
                     /translation="CHHASVRVDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDV
                     RLLRQEERERRKNIEKLIQIERNAKARIRRVTKYSPE"
BASE COUNT          558 a          508 c          384 g          574 t
ORIGIN      
        1 ctactacgcc accagtttca tctataaatc cctgaggtaa aattgccgct aaaatccttc
       61 tgtcctgttc cttcgatgtt gtccgctgaa caagcacaca ttcagatttt gtcacaagga
      121 aaccctgttg tggggttata tcgatatagg gaacatgatt ttttctgtct gcactaaaac
      181 gaaaaccagc tgaagttatt gattccgccc agactaatgg gtagctattt tttcctttcg
      241 tcgtacgcaa ctgcgactta aatcgattcc agactaactg acctgtagag actgagtatc
      301 ccagatcagc taaacgcgta gacatctgtt ttaatacatt aagaaaacag gcatcatctt
      361 taacgcgtgg caaaagccac gttgctccac ctttttctat atcaacatta cctattttct
      421 caatttttgc tttgttcaac cccttaggga caagtgagga tacctgggcc cgaactttat
      481 gttctcccgc cttgaatgct gtcaacattg tttcctggag aacatcgtca aagaccccat
      541 ccctgtcagt gacgaaatca agggcatacg gagtagtttt ttcggtcaaa agggttcgca
      601 gcgcggtgaa atactgccca ccaagaaaag aagttggcgt aagaaatgca ataactcctg
      661 ttttctcttt aaccatgcga acagctaagt cggtgaacaa tccatataga tttgcatgac
      721 caaataacga acgagaatat ttttctctcg ttttaatatc gagagtgaca cgtccgtaag
      781 gcggattccc taccacaaga tcataaccag aaaatttatc ttgctgaagc gcatcagcaa
      841 caataattgt atcttcaggc aatctacgtt ttacctttac acatagtggc attagtacag
      901 attcgagcaa taccaaactc atccaagccg caaatggatc gatctctatc ccctttaaac
      961 gacgactgat tctcctcaac atccattcag gggaagaccc cttgtctttt tttaacattc
     1021 gaatggctac tggtgccaaa aatgcaccgc caccacacgc aggatcaata accgatgcat
     1081 gagaaaaatc aacaccagac ttttcagcca ggtccaagag acgcgctacc aaaggtgggg
     1141 gggtatagta agcccccaat tccgaacggt aagcggatgg cagcattacc gtataaattg
     1201 acccaatcag atatccagcg tcctccgccg gaaattgagc aataagctca cctgttcgtt
     1261 cagcgagctc gcgtgcatca catggaatgt cttctatgta aacagtgtgc ggtactgccc
     1321 tcattttaat gctgcttttt tgcttttttg caagggtatc ccaatatgca gctacgatgc
     1381 tgaagcagta tgagcgggca tgcatcaacc ttgactgccc ggttttgtaa ttgcccgcat
     1441 aacctttggc catggcttta caagtttgat atctttccag attagaaaac tcattatctg
     1501 taaaaaaatc aaaatgttgc acattcataa tgtaaaatcc taatgtatca aacatgcggg
     1561 ccataacatc tcatggtggc gcatagcaaa ctggcccgaa gaatatcacg caccaagtcg
     1621 atctcataac ccgttatgac aataaatctt tgtagctgaa ctcacttggt gatttgtcaa
     1681 tcattcctgg cctccagttc gtcctgaatc tctttatcga gttcaccgta tttgttagtc
     1741 ctcatcactc acgacttgac ttccagaaat aaccttgatt attcggggct atattttgtc
     1801 accctgcgta tccgcgcttt cgcattacgc tcaatctgga ttagcttttc tatatttttt
     1861 cgcctttccc gctcctcctg gcgcaagagc cttacatcat ctgccagtct ggtttctctt
     1921 ttcgccacag agagcatcca gtcaaatggc tccacaactg caccgcaaat tttacagcgg
     1981 acctgacgct ctttttcgtc aacccggaca gaagcgtgat ggca
//
LOCUS       DAZOYL010000424         2012 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZOYL010000424 DAZOYL010000000
VERSION     DAZOYL010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.428"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(134..604)
                     /gene="argR"
                     /locus_tag="ACHUOY_004029"
     CDS             complement(134..604)
                     /gene="argR"
                     /locus_tag="ACHUOY_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR0684637.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            1039..1977
                     /gene="mdh"
                     /locus_tag="ACHUOY_004030"
     CDS             1039..1977
                     /gene="mdh"
                     /locus_tag="ACHUOY_004030"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0684638.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT          522 a          438 c          519 g          533 t
ORIGIN      
        1 accgtatgta ttcattgtgt gaatgatatg tcgcggtaaa acgcactatt cgttaaatta
       61 atcgcagggg aaagcagggg gttgagagga ataagcaaca ttttccccgc cgccagaagc
      121 gacggggcag agattaaagc tcctggtcga acagctctaa aatcgcttcg tacaggtctt
      181 tgacggtgaa accgttagca ggggtggtaa agatggtgtc atcgccagcg atggtgccca
      241 gaataccttc tgctttgccc agtgagtcca gcaggcgagc aattaactgc gccgcgccag
      301 ggctggtatg aatcacgaca actgcatcgt tgtagtcgat atccagcacc agattcttca
      361 atggactgga ggtggttggt acacccagtt cagctggcag gcagtaaacc atttccattt
      421 tggcattgcg tgtacgtaca gcaccaaact tggtcaacat ccgcgagact ttagactgat
      481 taatattgtc aaagccttgc tcctgcaacg cggcgacgat ttcgccctgg gagctaaatt
      541 tctcttcttt aagtaatgct ttaaatgctt taactagttc ttcttgctta gccgagcttc
      601 gcataagtca cccgatatgg tggttgatac aacattattg tgcatacaga tgaattttta
      661 tgcaaacagt cagccctgaa gaaggctgaa ataatgttat gaaagagcgg gattttatca
      721 aatttcgtta ttgagaaaca tgcctgcgtc acggcatgca aattctgttt aaaagtaaat
      781 taattgttat caaattgatg ttgttttggc tgaacggtag ggtatattgt caccacctgt
      841 tggaatgttg cgctaatgca taagcgactg ttaattacgt aagttaggtt cctgattacg
      901 gcaattaaat gcataaacgc taaacttgcg tgactacaca ttcttgagat gtggtcattg
      961 taaacggcaa ttttgtggat taaggtcgcg gcagcggagc aacatatctt agtttatcaa
     1021 tataataagg agtttaggat gaaagtcgca gtcctcggcg ctgctggcgg tattggccag
     1081 gcgcttgcac tactgttaaa aacccaactg ccttcaggtt cagaactctc tctgtatgat
     1141 atcgctccag tgactcccgg tgtggctgtc gatctgagcc atatccctac tgctgtgaaa
     1201 atcaaaggtt tttctggtga agatgcgact ccggcgctgg aaggcgcaga tgtcgttctt
     1261 atctctgcag gtgtagcgcg taaaccgggt atggatcgtt ccgacctgtt taacgttaac
     1321 gccggcatcg tgaaaaacct ggtacagcaa gttgcgaaaa cctgcccgaa agcgtgcatt
     1381 ggtattatca ctaacccggt taacaccaca gttgcgattg ctgctgaagt gctgaaaaaa
     1441 gccggtgttt atgacaaaaa caaactgttc ggcgttacca cgctggacat cattcgttcc
     1501 aacacttttg ttgcggaact gaaaggcaaa cagccaggcg aagttgaagt gccggttatt
     1561 ggcggtcact ctggtgttac cattctgccg ctgctgtcac aggttcctgg cgttagtttt
     1621 accgagcagg aagtggctga tctgaccaaa cgcatccaga acgcgggtac tgaagtggtt
     1681 gaagcgaaag ccggtggcgg gtctgcaacc ctgtctatgg gccaggcagc tgcacgtttt
     1741 ggtctgtctc tggttcgtgc actgcagggc gaacaaggcg ttgtcgaatg tgcctacgtt
     1801 gaaggcgatg gtcagtacgc ccgtttcttc tctcaaccgc tgctgctggg taaaaacggc
     1861 gtggaagagc gtaaatctat cggtaccctg agcgcatttg aacagaacgc gctggaaggt
     1921 atgctggata cactcaagaa agatatcgcc ttgggcgaag agttcgttaa taagtaattg
     1981 attagcgaat aataaaaaac cggagcacag ac
//
LOCUS       DAZOYL010000425         2008 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZOYL010000425 DAZOYL010000000
VERSION     DAZOYL010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.429"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>2008)
                     /locus_tag="ACHUOY_004031"
     CDS             complement(<1..>2008)
                     /locus_tag="ACHUOY_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR0684639.1"
                     /translation="LTEFLETLRIPKGREVTFSAFSDWLPRNRAIAALGAAHTLYEEF
                     RGVIGAVASGNGPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNL
                     LSHQYLSLATPRYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFS
                     WSSLKSLFFRQQQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFV
                     RSCGQAEGTIRLLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSV
                     QEVKGLEYETVILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKF
                     FTNALYVALTRATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHL
                     LEKQGKQEQADTIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSL
                     IYSLFPLYNALYREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHR
                     SPFNLTPLMSAARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQK
                     SAQLYTLLKPESLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAA
                     RLAECVVHLPEALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFL"
BASE COUNT          484 a          552 c          494 g          478 t
ORIGIN      
        1 cagaaaaagc ttttttccat aaggattttt gctgttaact tcatgttgag agagctggct
       61 gctgatataa ctccttcgtt tgcggcgttc cggcagcaac gcttcaggaa gatgaaccac
      121 acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa aacagagcac ttcccagatg
      181 cgtgtggaac agcgcctgca tcaggatcac cagaaacatt gccatctgcc ggttgtccag
      241 tttgatgagg cgtccttcca cctgcagtga cagactttca ggtttcagca gggtatacaa
      301 ctgcgcactt ttctgttgcg cataacgggg cgtactgacc gccgctgaca gaacttgatg
      361 ataagccgcc agcccgtcat taccggtcag caagggatct gcaccacgct ccagcagtaa
      421 ctgaaccagt gcaatattac ctgcccgggc tgctgacatc agcggagtaa gattaaacgg
      481 cgaacggtga tcaacaccat aacgttcaat atcccgcaac acggcaaccg ggttattcat
      541 tgagtatggc cggaaatact tcagctccag taactgcaga gtcttcgttc tgggctgcct
      601 tgtgggctta aaatcttccc ggtacagtgc attatacagc gggaacaggg aataaatcag
      661 actgtactcc agcgcctgca gttgtatggt tttatcggct gtacctgcga ggatatgctg
      721 tttccactgt cgtgcatcct ctgcggtgat aatctgccat ggcatctcag atgtctgcag
      781 gatccggcta cggatggtgt ccgcctgctc ctgcttcccc tgtttttcaa gcagatgtgc
      841 ggttttctgc cactcatcac ggctggaaat ctcttcctgc agattcagag gctcttcctg
      901 atgcgtgagc gcaagcagac tccacagagg atgctccacc tgctgttcaa caagatacac
      961 attgtgtgta gcacgggtga gcgcaacata cagcgcgttg gtaaaaaatt tatagatttc
     1021 cgctgaacga tcctgcctgt cccgcggacg ggaataacgg gcctcgccct caagatcagc
     1081 cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg gcagcgctta ctatattgta
     1141 aaggatcacc gtttcgtatt ccagcccctt aacctcctga accgaaaaga ccagcggcgt
     1201 actgaaccag catcgcgcct gtgctttctg ttccggatgc atcacaatga ccgctacacg
     1261 attcgaaagt gaggttttgg cattcagttc ctgctttgtc tcttccctgt cgtccagcaa
     1321 ccggatggtc ccttctgcct gaccgcagct tctgacaaaa tggtgacttt cccgatcaat
     1381 agcactaaag cggacctgtt tgagtctcag caaccggtta gccagtgcag tgacgtggtg
     1441 cccgttacgg taattcgcct gcaggatatt aacggttgtg tcgtttccct gttgctgccg
     1501 gaaaaagaga cttttgagcg aactccatga gaaaaagctc ggatgaacta tctgattggc
     1561 atcaccacag agtaaaaact gaccgggatg acgtagcgtt ttcagtacca gttgcagttg
     1621 aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca tcataacggg gagttgccaa
     1681 cgaaagatac tgatgactga gcagattact gtcatactga tgcgactgtt tcagccatgc
     1741 gatataacgt tcaaacagga catagaccgt cgggcggtcc tccatgccat agagcgactg
     1801 ccgtatcccc agtgacagat acgcctcccg ggacagcggc ccattcccgg aggcaaccgc
     1861 accgatcacg cccctgaact cctcatacag ggtatgggca gcccccagtg cggcgatagc
     1921 cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc acctccctgc ctttcggaat
     1981 gcgcagagtt tccagaaatt ccgtcagc
//
LOCUS       DAZOYL010000426         1996 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZOYL010000426 DAZOYL010000000
VERSION     DAZOYL010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.430"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..102)
                     /gene="prlF"
                     /locus_tag="ACHUOY_004032"
     CDS             complement(<1..102)
                     /gene="prlF"
                     /locus_tag="ACHUOY_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFR0684640.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALK"
     gene            complement(251..1822)
                     /gene="garD"
                     /locus_tag="ACHUOY_004033"
     CDS             complement(251..1822)
                     /gene="garD"
                     /locus_tag="ACHUOY_004033"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR0684641.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
BASE COUNT          465 a          533 c          491 g          507 t
ORIGIN      
        1 ttttaaggcc tcacgcactg gcgcggggat agttgtttgt ccgcgtatcg tgacctttga
       61 ttcagtggtc agtacagcgt gagagcgagc attagcgggc atgattcatt gtccttttac
      121 agcctgtttt ctgctcaaat tataagcttg aactaaggta atgcaaatgc attattaatg
      181 gacgtggggg ctttaaacaa taaaaacatg gcactaaggc catgttttgc gagggacgtt
      241 ccaaaagaaa tcaggtcacc ggtgccgggt taaacaccgc cagctggtta tgcagccccc
      301 attgatccga gaaggttttc ttcttcccgc tggcgacgtc gagaataaag tggaacaact
      361 tccagcccac ctcttcaatg ctctcttcgc cggtagcgat agtgcccgcg ttaatatcca
      421 ttaaatcaaa ccagcggttc gccagctcgg tgcgggttgc cattttaatt acgggtaccg
      481 ccatcaggcc gtacggcgta ccacggccgg tcgtaaacac ttgtacggtg atacccgaag
      541 ccacctgttg cgtgccacag acaaaatcgc tggctggcgt cgcggcgtaa attaatccgc
      601 gtttagtcgg gcgttgaccg ggcgacagca cttcaacaat tgcactttta cccgatttag
      661 caatggagcc gagtgccttc tccaccacat ttgccagacc gcctttcttg ttgcccggcg
      721 aagggttggc gctgcggtcg gtttttccca tattgagata gttatcgtac cactccatct
      781 cctccagcag ccgtttgccg acctcttcgt tcactgcgcg tggtgtcagc agatggatcg
      841 cgtcacgcac ttccgttact tctgaaaaca tcaccgttgc gccgcagcgc accagtagat
      901 cagacgcata gccaaccgcc gggtttgccg ttacaccaga aaacgcatcg ctgccaccgc
      961 actgcacacc aacgaccagt tctgaagccg ggcaggtttc tcgctgccgt tgattcagtt
     1021 tttgtagatg gcgttcggcg acctgcaaaa tatcctcgac catggactga aagccgacat
     1081 gcttttcatc ctgcaaactg acaatgctgg cgctttctac tggaatagct tgcacatcat
     1141 ccgtgccgac cagcaggcgc tcaggctgca acttttcaca acccagacca atcaccatta
     1201 cttcgccgcc aaagttaggg ttcagcgaaa tattgtggat ggtacgaata ggcacaactg
     1261 ccgccggtgc gttaatcgcc acgccacagc cgtacaaatg attcagcccc accacgccat
     1321 cgacgttcgg gtatttcggt agcagatcgc gttcaatgat ttttactaca tagtccacca
     1381 cgcctgccac acagtggacg ctggtggtga tgccgagcag gtttttggtg cccacgctgc
     1441 catcggcatt gcgatagccc tcaaaggtgt atccttccag cggcggtaag ggggccggga
     1501 ctttggttgc cagtggcagc gtgtgtaacg gcggcgcttc cggtagtaca accattgatt
     1561 cgtcgatcca gcttccgcgt gggattgcgc gcacggcgta accaatcact tcgccataac
     1621 gaataatttc accattagct ggaatgtcca gcaatgcgac tttatgcccc tggggagtat
     1681 gttcaattaa ttccagccca tccggaaaac gcgttcctgc tttcaggcca ttatcattaa
     1741 caataattgc cacattatct gtgtcgtgaa cttttatata aaacgcagtt ggcgtttctt
     1801 gtctgatttc gatgttggcc attgtgcaat attctccagc caggggttat taaaatgagg
     1861 atctggtctc atttaaaaat aaatgacttc attgggtgaa ataagaatgt caggagttta
     1921 aacgcagaaa tgtgatcaat gtcaatcatt tcagggattg cagtgccgcc cggtaaggta
     1981 aagcgcaaat ctgtgc
//
LOCUS       DAZOYL010000427         1956 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZOYL010000427 DAZOYL010000000
VERSION     DAZOYL010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.431"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..516
                     /locus_tag="ACHUOY_004034"
     CDS             <1..516
                     /locus_tag="ACHUOY_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0684642.1"
                     /translation="IRIGCMKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIM
                     GFSTQGAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQD
                     NIVALPADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVA
                     RWLAAQKGRRA"
     gene            536..730
                     /locus_tag="ACHUOY_004035"
     CDS             536..730
                     /locus_tag="ACHUOY_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR0684643.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            730..1020
                     /locus_tag="ACHUOY_004036"
     CDS             730..1020
                     /locus_tag="ACHUOY_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0684644.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            1300..1452
                     /locus_tag="ACHUOY_004037"
     CDS             1300..1452
                     /locus_tag="ACHUOY_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0684645.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            1464..1829
                     /locus_tag="ACHUOY_004038"
     CDS             1464..1829
                     /locus_tag="ACHUOY_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684646.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT          594 a          438 c          456 g          468 t
ORIGIN      
        1 attagaattg gatgtatgaa aagaaaatca ttgtcagaca ccgacctcaa cgccgccaga
       61 agactgaaag aaatctggat ggcaaagaaa tctcagttgg gattgactca ggaacgtgcg
      121 gcggaaatta tggggttttc aacacaaggg gcggtaagtc actacctaaa tggtcaaacc
      181 ccactaaacc ttgaggctgt tctaaagttt gctggcttac tgaaagttcc ccctgagtca
      241 atcaggccag acatggtaga tttgctgcaa attgcaagga tgtattgtcg agaaccacag
      301 caagataaca tagtcgcatt accagcagac acagaacaaa cagaggatga actaccgttt
      361 gccgtagagc cgatggaacg agatttaatc catacgttca gagcttttcc gaaagaagat
      421 caagaacaca tgcttcagga gatgaaagat aaaaaagaat caatggatcg tacggttgcg
      481 cggtggttag ctgcacaaaa aggccgtcgc gcctaaatca gaggagatat gtaaaatgag
      541 tacagtcctt tccccaatgg tttcagaatt tgagacagtt gagcaagaaa atagttacaa
      601 cgaatggctg cgggccaaag tagcagcaag cctcgcggat ccccgcccgg cgattccgca
      661 tgatgaggta atggcggaaa tggaacaaat tattgcccaa attgctgctg caactcacag
      721 aggtgaataa tgttacccgt tttatggcta ccatctgctc gtgatgattt gcgccagatc
      781 gtagcctaca tagctaaaga aaatcccgcg gcagcacgca gaatgaaaat tcgaattgaa
      841 acatccgttt tgccgctcac agagcatcct tacctgtatc ctccaagcga gcgagttctt
      901 ggtcttcgtg aaattgtaac tcatcctaac tacatcatcc tttaccgagt aacagcatca
      961 agcatagaag tagtaaatat tgtgcattct cgaagacagt atccaggcaa aaacagttaa
     1021 ttattcccgc cgacagaacc accttagggt ggttttttct tgccccaaca aaaaacaata
     1081 gcgatgctat ttacatcaaa aaaaataatg atgctattgt ttcaggcgtc aacccacccc
     1141 gccccacaga atgcagggca atacttcgag ttaccagaca gtggtcaggg gttaagtagc
     1201 cagcccgagg cgtaagaaca tgacggcagg gttcaatctt taactatgca gcaggttttt
     1261 tgttccgcca ccccggcgtt aaggggaaat gaggtcagca tggatactat cgatcttggc
     1321 aacaacgaat ctctggtgtg cggcgtgttc cctaaccagg atggaacgtt taccgccatg
     1381 acgtatacca aaagcaaaac gtttaaaacc gaatctggcg cgcgttgctg gttagccaga
     1441 aacactgact gatgaggctg acgatggaat ttaaagattt accgtctgat gtacagaaaa
     1501 cagcagctca tacattgcat tctgtgctgc gagaaatcgg gaaagatatt gcaagcgagc
     1561 cagcaaaaga tctggcccgg aaaatcaaga ccgctttcgt tgagctttat aatgttggca
     1621 ctgactctga aactgtcgag accaagaccg taagttcacc aatattctca cttgagccaa
     1681 gcgtattatc aggtgaaata tgcaccgaga tttccagcga actgcttccg gtaatacgag
     1741 aagcaattcg ccgtcgcggt ttggatggaa gttatgatca tgacgttctg caagttctca
     1801 ggacaatggt gacttcactg gggatttgat ccctgcgtcc tctaagcttt tgatatagcg
     1861 gtcctccaag aagcggtaaa ttttgtcgaa atcatctttt ccctctggtt tattcagagg
     1921 attacaggac ccgtaagtag cggcatacat ttcaac
//
LOCUS       DAZOYL010000428         1951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZOYL010000428 DAZOYL010000000
VERSION     DAZOYL010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.432"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..612)
                     /gene="yeeO"
                     /locus_tag="ACHUOY_004039"
     CDS             complement(<1..612)
                     /gene="yeeO"
                     /locus_tag="ACHUOY_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR0684647.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILN"
     gene            773..848
                     /locus_tag="ACHUOY_004040"
     tRNA            773..848
                     /locus_tag="ACHUOY_004040"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:806..808,aa:Asn,seq:gtt)
     gene            complement(886..1836)
                     /gene="cbl"
                     /locus_tag="ACHUOY_004041"
     CDS             complement(886..1836)
                     /gene="cbl"
                     /locus_tag="ACHUOY_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR0684648.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          551 a          504 c          421 g          475 t
ORIGIN      
        1 attaagaata ttcaggctac cgttaatcaa tagcggtatt ttcgtattcc ctgcaccacg
       61 aagtgccccg ctaccaataa gagtgatggc agctgctgga taactgagta ccgtcagctc
      121 cagataagtc aacgccagtg ctttaacttc tgtcgtggca tcacccgcga cgaaatcaat
      181 aatttgttcg ccaaaatgat gaataagcgt tgccaacagt acggcaaaca acgtcatgat
      241 caccaatgac tgccgcgtcg ccaccctcgc tcgtcgtcga tcccgcttac cgagactaaa
      301 tgccacaacg acagtagtac caagatcgat agcagcaaaa aaagccataa tgaccatatt
      361 gaagctgtcc gccaatccca cgccggccat cgcatctttt cccagccagc tgaccagaaa
      421 agtgctcaga acccccatca acaggacaca ggcattctcc atgaagatag gaacagcaag
      481 cggggttatc tcgcgccaga acaacacttt gtagctcttg cgtttagcgt gccagcgagt
      541 gccgtgaaca acctggcgta aagcagagga gatattcaaa gccgacctta attgcagaaa
      601 gtgaaaccac atttcaaata atgagggaga atcagcaaag ctgcaaagat tttcgccaac
      661 aaattgtctg caaatgcaac aaactgttga tagaaacggc aaacagttgg ggaatttaaa
      721 aatcgggttt gacaaaagat ttttcgccgt taagatgtgc ctcaacagcg attcctctgt
      781 agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag
      841 aggagccaaa tttctgaaaa gcccgctttt atagcgggct ttttgctata tctgataatc
      901 aatttcctct tcactgtttt ccatcacctg ccgcttgata tcctcaactg acagtcctgc
      961 attacaaagt tccagaaagc gccagacata gttacgctga agttgtcctc gcttcagtcc
     1021 caaccagaca gtattagcat cgaaaagatg ccgcgtatcc agacggatta aattctcttc
     1081 ctcttgttcg ccgctggatt gttcggcaac taatccgatc ccaagcccaa gagcaacata
     1141 ggttttaatg acatcagaat cctgcgcact taatacaata tctgccagca aacctttgcg
     1201 ggcaaatgcg tcatcaatac gtgagcgccc cgtaatcccc tgtcggtaag tgattaacgg
     1261 ccacttcgct attgattcca gcgtcaatgg tgtaatttgc gttaagggat gatcgagtgg
     1321 aacaagcaaa ctatggtgcc aacgaaacca cgggaaggcg acgagctgcg ggtcattact
     1381 caaacgctcg ctggcgatac caatatcagc ttcgccattt tgcaacaacg tcgcaatttc
     1441 ctgtggcgtc ccctggatta gctcgagccg aacctccggg aaaagttcgc gaaaagcttt
     1501 aatgacctct ggcaagctat aacgtgcctg agtatgcgtc gttgcaatag tgagaacgcc
     1561 agacgtatcg ttggtaaaca ggtctgcaag ccgacgaaca ttactggctt cattcagaat
     1621 acgttctgca atgaccagta atgctttgcc cggttcagtc atgcccagca gtcgcttacc
     1681 tcgtcgaaca aatatttcga tgccaagttc atcctccagt tcccgaatat gacggctgac
     1741 gccggactgt gaggtaaaaa gcatattcgc aacctctgtc aggttgtaat cctgacgtgc
     1801 agcctcgcgg attatcttta gttgttggaa attcacggta aactccgggc agttcagatt
     1861 tcccgttatt gttaaagtct aatgcccggc ataacaaata ataaaaaccc gcatcttatt
     1921 ccatcccgat ataacgctta gctcaccaat t
//
LOCUS       DAZOYL010000429         1939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZOYL010000429 DAZOYL010000000
VERSION     DAZOYL010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.433"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUOY_004042"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUOY_004042"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR0684649.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHUOY_004043"
     CDS             1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHUOY_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR0684650.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSV"
BASE COUNT          510 a          451 c          456 g          522 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgt
//
LOCUS       DAZOYL010000430         1933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZOYL010000430 DAZOYL010000000
VERSION     DAZOYL010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.434"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..100
                     /gene="mgtT"
                     /locus_tag="ACHUOY_004044"
     CDS             <1..100
                     /gene="mgtT"
                     /locus_tag="ACHUOY_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR0684651.1"
                     /translation="GDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHUOY_004045"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHUOY_004045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR0684652.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="ACHUOY_004046"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="ACHUOY_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR0684653.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          559 a          450 c          376 g          548 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cag
//
LOCUS       DAZOYL010000431         1922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZOYL010000431 DAZOYL010000000
VERSION     DAZOYL010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.435"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHUOY_004047"
     CDS             <1..184
                     /locus_tag="ACHUOY_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684654.1"
                     /translation="TKIKTTIITICALSYNLTAQASPSYRPRDRVNKTMIELQTNGVE
                     RQLLDNSCGISSGVFK"
     gene            complement(202..537)
                     /locus_tag="ACHUOY_004048"
     CDS             complement(202..537)
                     /locus_tag="ACHUOY_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFR0684655.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            666..1013
                     /locus_tag="ACHUOY_004049"
     CDS             666..1013
                     /locus_tag="ACHUOY_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR0684656.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1033..1623)
                     /locus_tag="ACHUOY_004050"
     CDS             complement(1033..1623)
                     /locus_tag="ACHUOY_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684657.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
     gene            complement(1620..1880)
                     /locus_tag="ACHUOY_004051"
     CDS             complement(1620..1880)
                     /locus_tag="ACHUOY_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684658.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          474 a          513 c          455 g          480 t
ORIGIN      
        1 cacaaagata aaaacaacta tcattacaat atgtgcatta tcatataatc ttacagcgca
       61 agcttccccg tcatacagac ctcgtgatcg agttaataag actatgatag aactacaaac
      121 taacggagta gaacggcaac ttttagataa ttcttgtggc ataagttctg gggtatttaa
      181 ataaaatata agacaggctg tctatcttac agacagcctt tttataataa cagaaagaat
      241 atatatcata cttaacggaa gagataatat gaaaacagta cccgtataaa cagcaagcaa
      301 tcccattttt ccaggtgtat cagcaaaaaa aacgctgttc cagaaatcag gccgggtaaa
      361 tttcagagct gtatcttcaa tataccattt tgcaaccgga tacaaaacca ttccgcagag
      421 agaaatacac cagaacagta atctgtattt aaaatcataa tcccatgaca tatacagcat
      481 atatcctccc gtcacccatc cccaccacat attattaaaa taatattttc tgttcatctg
      541 ccagttttcc tttcttttgc acttttgtca gtgtactgat gcatgacaac ataaccacag
      601 catgataata ataatcaata acaataagct gtgtcacgtt tacatcatgg aacggaggtg
      661 ctcctgtgac ttttgagcag aaaaaagccc gggccattgc cctgatggac agcaaaaaga
      721 tgtggcggag caattatgcc cctcctcttc tgcgtattct ctggcggctg ggtatccggc
      781 ttccgccatt accctttatg ccgttctggc aggtcacggt gctgacgggg ggcttatggg
      841 gcatttcctg gggatgtgca atgtggttta tctactgggg gccgtcagga atggtggcag
      901 gtgaggccat tattataagt atcacggggg gttttttgtc cggactgctc atggcctctt
      961 tccactggtg gcgcaggaaa gttaaccgac tgccaccatg ggatgatgtg taaaccgtgc
     1021 acaggcgtgt tcttacggag cggcactttc atttatctgt acgcgaacct ccattcctgc
     1081 atcgtgacag gccggcagcc actgcgccac ttccagcgga tcgccctccc ggcgtaccac
     1141 ccagccttct ttattccata actgcagaca ggtgctgccg tcgagacgca ccacaaaatc
     1201 cccacggcag gcctgatacg ttatgccgtt cagctccacc gccagatcag tggcagaagt
     1261 gccgcctgcg ggcagacctg ctgccgggac cagcggcagt acgacgactt ctgccgcacg
     1321 ctgttctgcc cggatgcggt cctgttctgc cagcagcaga ggctgtgcca cggcccggcc
     1381 ggcgtccgtc agctccactg ccagctgcag gttcggagcg cgcagggtac gcagccagcc
     1441 ggcatcctcc agacgacggc aggaggcgcg caggtttggc ccgtagaccg gggcttcccc
     1501 gctttgctcc agcacccgtt ctatgtcccg cgtcagcacg ggtcctggcc gtttttcatc
     1561 aagggcagcc agcacaatca gcactctccg ctgcagtggc gacggtcggc ggctctgagt
     1621 catttccagt catcctccgg ccagatgagc tttctgaggc gctgatggtc ggcgtcaatg
     1681 atctcctgtc tctttatggc attcattgag gtatagccat ttatggccag ttcatgccag
     1741 aagatcagca cggctccggc tttggctgac tcgcagagat aagttacgcc gtggctgtcc
     1801 agattgcatt cccggatgcg tttttccgca aaccgggtca gttgttcata gctgagtgaa
     1861 aacgtcattt cctgatccat cctgcacgtt ccatctgata atgttctgac aggtaaaaat
     1921 ca
//
LOCUS       DAZOYL010000432         1914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZOYL010000432 DAZOYL010000000
VERSION     DAZOYL010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.436"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            263..1138
                     /locus_tag="ACHUOY_004052"
     CDS             263..1138
                     /locus_tag="ACHUOY_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR0684659.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1146..>1914
                     /locus_tag="ACHUOY_004053"
     CDS             1146..>1914
                     /locus_tag="ACHUOY_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR0684660.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKL"
BASE COUNT          550 a          438 c          441 g          485 t
ORIGIN      
        1 atcggggcgc gggaatgaaa tcccattcag actccggata gattcaagca agcccactta
       61 tcgtcaaaat cggtgttgca aaaacgggag tgaccataga tccgttttcc aagcggaccc
      121 cataatatcg gctggtaaac atcactcgct agtgcattct ttttgttggt aatgataatc
      181 atttacattc attctttgat gttttatcat aattaatgtt ttgtcaataa atatatggaa
      241 acatattgtt tcagatggga atatgagaaa gttcatcctt atttctatga taacgttagc
      301 tagtgttagt ctgggagcct gcagacagaa tgtcaccata aagcaggatg cgccagggca
      361 gaaagctttc ctgactgacg ggcagattta cgatttgcac agtggcaaaa ttatttctag
      421 cagcgagctt ttggcggatc ttgctaccgc tcgacatctt attattggtg aaaaacacga
      481 taatgccgag catcatcaga ttgagctttg gcttattcaa aatttgctca tccagcgacc
      541 acaggggagt gtgttgcttg agatgttgac gtcagagcaa cagccccgag ttaaccaagt
      601 taaatgttgg ctgaaagata atcctgtggt tcgagatagc cgtgttcagg aactacttaa
      661 ctggcaaaaa gggtggtcct gggagatgta tggcgacatc gtcatgcagc tattacgtgg
      721 gccttacccg ctgctcaatg ccaacattgg ccgggagcag atgttggcac tgtacaaaaa
      781 aaacgaattt cctaaaggaa aaaaatccac tgcacctgtg gtacaagaag ctctgcgtga
      841 aaccatcata tctatgcatg aaggcaacct tgaaagccag caactgacgt caatgctctc
      901 tattcagcag cagcgggatc gctacatggc ccgccagctt ctgagtgccc cggtaccgtc
      961 gttgttgatt gcaggtggtt atcacgcgtc aaaaagcatg ggcgtaccgt tacacatgga
     1021 agatcttgcc accggcaccc atccagtggt gttgatgctt gcagaaaaag gaatgaacat
     1081 cacagtagat catgctgact acgtgtggtt tgtagcgcca gatacaacaa aaaggtaaac
     1141 catggatgat gaatattctc cacttctcac agtcggtaaa atggagcagc tggtttatct
     1201 gttctctact gcttcatgga ctgattttcc ttgcgtttat ctggcgtttt tccgaagttc
     1261 agcccgctat gtctcctgca ccggcgatca tgctgcaatg ggcagaaatt atagaggctc
     1321 cttcttctcc actttcgctt ccggtgggaa tcgctcaaca ggagtccgca gtgacagaag
     1381 aaaagcaaca aactgaggac agacaacaac gtcctgtcac tgaagacagt gatgcaacca
     1441 ttgaaataac taggaagaaa aagtcatccg atggagaaaa gaaaaagaca cgtccacctc
     1501 gtaagataaa ggctcaaacc agcgacagca accccacagc cgtttccagt aatgctgcac
     1561 ctcaggctct ggtcgaatcg tcccgtattg ctgcgccttt taacagtgac tcaacaaagc
     1621 gagataattc tgaggcatct tgggaaagca gagtaaaagg ccacctaaac cgttataaac
     1681 gttaccctgg cgatgccaga aaacgtgcac gaaccggcac agccgtcgtg acttttaccg
     1741 taaacacgga aggcactatt gtcagtagtt tcttggaaat ctcttcagga accctttctc
     1801 ttgaccgcga agctattgct gttttagaac gcgctcaacc acttcccaaa ccaccgcctg
     1861 aaatattgga gggaggatta tttaaagtaa aaatgccgat tacctttaag ttga
//
LOCUS       DAZOYL010000433         1899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZOYL010000433 DAZOYL010000000
VERSION     DAZOYL010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.437"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(89..643)
                     /locus_tag="ACHUOY_004054"
     CDS             complement(89..643)
                     /locus_tag="ACHUOY_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0684661.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="ACHUOY_004055"
     CDS             complement(640..930)
                     /locus_tag="ACHUOY_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0684662.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="ACHUOY_004056"
     CDS             complement(930..1529)
                     /locus_tag="ACHUOY_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0684663.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="ACHUOY_004057"
     CDS             complement(1601..1813)
                     /locus_tag="ACHUOY_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684664.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          472 a          501 c          387 g          539 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
     1861 aaaaggtgat gctgccaact tactgattta gtgtatgat
//
LOCUS       DAZOYL010000434         1898 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZOYL010000434 DAZOYL010000000
VERSION     DAZOYL010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.438"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..749
                     /locus_tag="ACHUOY_004058"
     CDS             <1..749
                     /locus_tag="ACHUOY_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFR0684665.1"
                     /translation="SKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYEL
                     DKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEV
                     NAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLN
                     FDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASL
                     QLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(790..1722)
                     /locus_tag="ACHUOY_004059"
     CDS             complement(790..1722)
                     /locus_tag="ACHUOY_004059"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0684666.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
BASE COUNT          610 a          411 c          404 g          473 t
ORIGIN      
        1 agtcgaaatt taatcagatt aacattatca atcacgggga agacgttgcc caaatcgatg
       61 ctttcgttgc aaaaaccatg ctggatcgcg ttaaagacaa agattatatc aatgtcaatc
      121 tgacctacga acttgataag ttaacaaaag ggaatcagca actcggtagt ggtgagtggt
      181 cattgattgc tgaatctatt gatccctcag cagtgcgcca atttatcatc cagtataaca
      241 ttgctatgca gaagcagctt gctgtacacc ctgagttagc aaacgatgaa gttgctctgc
      301 aagaagtgaa tgctgcattg ttcaaagagt atttaccgtt attacaaaaa agtgagccga
      361 ccattaaaca accggtaaga tggaagaacg cactcggcga actaaatgcc aatctggata
      421 tcagtattgc cgacccagcc aaatcttcat catccacaaa caaagatatc aaattgctca
      481 attttgatgt gaagttaccg cttaatgtcg tcacagaaac cgcaaaacag cttaatttat
      541 ctgaaggaat ggatgcggaa aaagcgcaaa agcaggctga taaacaaatc agcgggatga
      601 tgacattggg tcagatgttt cagttaatca cgattgacaa caataccgcc tcgctgcagc
      661 tgcgttatac accgggtaaa gttgtgttta acggacagga gatgagcgaa gaagaattta
      721 tgtctcgtgc cggacgtttt gttcattaaa atactcactc gcctctgctc attcagaggc
      781 gagtaaaact tacttattct gacgctgtga tgacatgaga gacgtcagaa gccggtcttt
      841 ttcctgccac aatgagttca gccattgctg aaaatggcgc ttaaagcttt tgtcattgat
      901 gtagtcgcca tgtaactcat cggcaatagg ctgtaaatcc acatgtacga caatccgcgt
      961 cagtttaccg cttaacatat cgaagaacgg ctgacggtta ttgtccggat aacacagcgt
     1021 aacattaagc agtttatcga attgtttacc cagtacattt agcgccatcg caatgcctgc
     1081 cgcctttggt ggcaacaagt tttgaaaagt ggagtgggtt tgctgatgtt tttcttgcgt
     1141 gaaacgggag ccttcaacga aattgacaat ggtggtggga tgcaggcgaa acttttcaca
     1201 agaacgccga gtggtttcaa catctttacc acgtcgctcc ggatgacgta ataaataagc
     1261 gcgggaataa cgcttcataa acggcatatc cagcgcccag cacgccaggc caagaaatgg
     1321 cacccaggcc agctgttgct tgaggaaata tttattcatc ggaatgtgct tacgaaacag
     1381 cacgcacagt acgacaatat ctgcccaact acggtggtta caaataagca gataccagtt
     1441 cttcttactt aacccttcca gcccgtgaac ttcccattgc aggtgtgggt tcagatgcag
     1501 taataccgcc agaccttcac accagcaata catcataaaa tcacaaaaac gcgaaacctt
     1561 tcgccaaatg actggcacag gtagcaatag ttttacaatc ccggcaatga tgatcgggac
     1621 ggaacaaaaa atagtgacca aaatggtcaa tacgatactc agcaaaaggg ttatcgcagc
     1681 gagtattctc gtcataatga atttattcaa aaggttagcc atagacagtg cgctaaagaa
     1741 gcgcaaggcg cagattttag cagaaaacga cccaaataac ggatgatcct taaggagaaa
     1801 aataatccat atctatccac attagaaaaa atcccattat ctcaattatt agggatggct
     1861 ttatttttaa ctgcatgaaa aacaaagaca aacatcat
//
LOCUS       DAZOYL010000435         1877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZOYL010000435 DAZOYL010000000
VERSION     DAZOYL010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.439"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..228
                     /gene="repA"
                     /locus_tag="ACHUOY_004060"
     CDS             <1..228
                     /gene="repA"
                     /locus_tag="ACHUOY_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR0684667.1"
                     /translation="LSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEIKVFVKNPLKDL
                     MVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            452..526
                     /gene="tap"
                     /locus_tag="ACHUOY_004061"
     CDS             452..526
                     /gene="tap"
                     /locus_tag="ACHUOY_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR0684668.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            519..1376
                     /gene="repA"
                     /locus_tag="ACHUOY_004062"
     CDS             519..1376
                     /gene="repA"
                     /locus_tag="ACHUOY_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0684669.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESDAGTLSITRATRALTFLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDV
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          488 a          494 c          477 g          418 t
ORIGIN      
        1 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
       61 gccgcagttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
      121 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
      181 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
      241 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
      301 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
      361 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
      421 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
      481 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
      541 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
      601 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
      661 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
      721 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt ccactatgac ccgctggcca
      781 accgcgttca gtgctccatc accacgctgg ccattgagtg cggactggcg acggagtctg
      841 atgccggaac actttccatc acccgtgcca cgcgtgccct gacgttcctg gcagagctgg
      901 ggctgattac ctaccagacg gaatatgacc cgcttatcgg ctgcaacatt ccgaccgaca
      961 tcacgttcac accggcgctg tttgccgccc tcgatgtatc agaggaggcg gtggcgtccg
     1021 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa gaagcaggga ctggataccc
     1081 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt gcgtgagcgt ttccgcagtt
     1141 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     1201 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     1261 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     1321 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     1381 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     1441 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc tggtgcatcc
     1501 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     1561 ttaaactgca agggacttcc ccataaggtt acaaccgttc atgtcataaa gcgccagccg
     1621 ttagctttac agggtgcaat gtatctttta aacagctgtt tatatctcct ttaaactact
     1681 taattacatc catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacagatat
     1741 gcacctccca ccgcaagcgg cgggccccga ccgaagccga tttagttaca acatgcaaac
     1801 ataaacccct aaaaaaccca agtccgacgc agaactgaaa ccacaacgcc cctcctcata
     1861 actgaaaagc gccgctg
//
LOCUS       DAZOYL010000436         1868 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZOYL010000436 DAZOYL010000000
VERSION     DAZOYL010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.440"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..649)
                     /locus_tag="ACHUOY_004063"
                     /pseudo
     CDS             complement(1..649)
                     /locus_tag="ACHUOY_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(852..1661)
                     /gene="pduF"
                     /locus_tag="ACHUOY_004064"
     CDS             complement(852..1661)
                     /gene="pduF"
                     /locus_tag="ACHUOY_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR0684670.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT          603 a          446 c          378 g          441 t
ORIGIN      
        1 ctcacttgct gtcgatacac ctgcggcgta acctgataag tctgacggaa aactttgcaa
       61 aaataactgt attgtcgtgg ggattaggcg cacgagtctg gcgcaccgaa tctgaccgag
      121 atttgttctc tttaataacc acaaaattga gttgtttttt gaggcaattt tccacaatca
      181 acttcaacaa atcagcagag gccataactc ttgagtaatc catctcagga actttgcgga
      241 attcattaag taattcggga tctgcctgcc agcaatcatc gacattcaga atatcgacca
      301 aatcgacatc atcattacgc agacgaactt gcccgcacaa tacaaaaccg actaaatgtc
      361 cggcgattac cagtggaata gagaaatcgg ttaatccggc atgacagcga taaatacaag
      421 gctgatcgga tttagaggct tccaggcccc cacaacgatc gctcatgcga catcgagtgc
      481 ttagctgagg gtgttgccgc atcatctgac aaaatggagt gaagttaaac agctcggaaa
      541 tttcatcgcc gtgaatattg accacgacaa ccgcaaggct ggtcgcctgc gcaaaatcct
      601 gcgcgatttt attaatgagt tctgaattaa gtgtcgccgc agaaatcatg ataaaacctc
      661 ccagttaatt tattgttata gcaaaacaat tgttttacat ttaatcttca catcatccct
      721 gatgatttac tgacgagaag cacaaaggac aataacaaaa gcctccgtga agcgggagac
      781 tttttaataa gttcggaaag cagatcacaa acagtaaaaa aataaatgtt tataactgcc
      841 agagtatttc gttattcgct aattccttta ggtgtaacac tattgacagg taaattcttt
      901 gcaatgagat agcgataaat cgccgcacca acacatgccc caatgatcgg tgcaataatg
      961 ggtacaatga aataaggaat attgcgtcca ccagtcatcg caatttctcc ccaccccgca
     1021 agccaggtga aaatcttagg tccaaaatca cgcgctggat tcatggcaaa accggttaat
     1081 ggtccggtag aagcaccaat aacagcaacc agaataccaa taagtaaagg tgcaagcggt
     1141 cctttgggta cgccattacc gtcatcggtc aacgccataa tcaaccccat taacatggac
     1201 gttatgacca cttcgaccaa cgcagcttgc catacattaa tcgccgctgc cggataagta
     1261 ctgaagatac tggcgagttg taaactttct gcgctaccac gcaccatatt gtgagcagat
     1321 tcaaaatcgg taaacagact actgtataga gaatatgcca gaactgcacc accaaaggca
     1381 cccgctaatt gcgccagaca aaatggcagc actttacggg ctgggaaaga ggcaaacagc
     1441 cacagagcaa tggtaactgc cgggttgaga tgagcgccgg aaatacctgc cgtaaggtaa
     1501 acagcaagcg aaatccctaa cccccaaatg atacaaattt cccacagtcc cagacttgcc
     1561 ccggcaactt tcagggcgca caaacaacca atgccaaaaa agaggaacag tccggtgccc
     1621 aaaaactcgg caacgcattg cgccttgagt gaatcattca tagtgacacc ttttttacgg
     1681 agtttttctt tatcagccaa ctatgcgctg actgttgtat ataccgagca caactataaa
     1741 aaattgctct gcagaaaata ttgccaacat gcatgacaga ctcgtgtaaa aatcgtgcta
     1801 acataacaaa ttattgtcat taccgaatca tgcaacacag cgttagtcag tgttgaaagt
     1861 aataattc
//
LOCUS       DAZOYL010000437         1842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZOYL010000437 DAZOYL010000000
VERSION     DAZOYL010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.441"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(50..184)
                     /locus_tag="ACHUOY_004065"
                     /pseudo
     CDS             complement(50..184)
                     /locus_tag="ACHUOY_004065"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..1018)
                     /locus_tag="ACHUOY_004066"
     CDS             complement(197..1018)
                     /locus_tag="ACHUOY_004066"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0684671.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNESILLNGKTIRKLMKELGISSLIRRKKYRTYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1007..1813
                     /locus_tag="ACHUOY_004067"
                     /pseudo
     CDS             1007..1813
                     /locus_tag="ACHUOY_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1287..1403
                     /locus_tag="ACHUOY_004067"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          464 a          408 c          440 g          530 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaat tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac
     1741 ttacgtcagc acatggcagg gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg
     1801 atacatcgtg tggtgacgag tctcatcgtc tatggaaacg ac
//
LOCUS       DAZOYL010000438         1832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZOYL010000438 DAZOYL010000000
VERSION     DAZOYL010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.442"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(75..1583)
                     /gene="putP"
                     /locus_tag="ACHUOY_004068"
     CDS             complement(75..1583)
                     /gene="putP"
                     /locus_tag="ACHUOY_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR0684672.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
BASE COUNT          509 a          550 c          405 g          368 t
ORIGIN      
        1 tttatatggc gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc
       61 gcaggctaag tcccttagtt ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca
      121 tcggcctcgg caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac
      181 actacaatcc cgatactgcc gaagatgaag cccggaataa tttcgtacag tcccagccag
      241 ccgaactgct tccagacgat aactgtcagc gcaccgatga tcatccccgc cagcgcaccg
      301 ttgcgcgtca tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca
      361 aagcctgccc acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc
      421 gcaatcgcca ccagcgccac caccagcacc atcacacgtc ctacccacac cagctctttc
      481 tggctggcct gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac
      541 accagcagct ggcaacttaa ggttgacatt accgccgcca gaatcgccga cagcagaatc
      601 ccggcaatcc acgggttaaa cagaatttgc gccagttcga taaacacgcg ttcagcgttc
      661 tggtttaccg cacctgctac cgccggatgc tcgttaaagt aagcgatacc aaagaagcca
      721 acagccaccg cccctgccag gcaaaggatc atccaggtca tactgatacg acgcgcatgg
      781 acaatgctgt ggtgagaatc tgccgccata aaacgcgcca ggatgtgcgg ctgcccgaag
      841 taacccagcc cccaacccat caatgagata atggcaacaa agttcagccc tttgagcata
      901 tccacgtttt cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca
      961 ctgataatga cgataaccgg cgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca
     1021 gtgtcagtcc agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgacgca
     1081 gctcccgcca acagagccgt ttcgtaactc atgccaaagg tactttcaaa cagacgcgcg
     1141 cctgccacaa tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag
     1201 ataatgcgca aaatgcggct tttatcttca aaacgcccgg tgaaataatc cggcaatgtt
     1261 aaggcgttat tattgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg
     1321 atccacgcgc ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa
     1381 accgcgccag gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat
     1441 gccgtcacga atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt
     1501 gatcgccagg cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc
     1561 aacatcggtg tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa
     1621 acttgccgtt atatctgcca ccggaacggg gtaacagagt ttatgtttta ccagggcgac
     1681 cgtatcctgc cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat
     1741 tttagtgctc agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg
     1801 cacctttcaa aaaggttaac tgccgcagag aa
//
LOCUS       DAZOYL010000439         1818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZOYL010000439 DAZOYL010000000
VERSION     DAZOYL010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.443"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHUOY_004069"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHUOY_004069"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR0684673.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1006..1416
                     /locus_tag="ACHUOY_004070"
     CDS             1006..1416
                     /locus_tag="ACHUOY_004070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684674.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(1450..>1818)
                     /locus_tag="ACHUOY_004071"
     CDS             complement(1450..>1818)
                     /locus_tag="ACHUOY_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0684675.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgc
//
LOCUS       DAZOYL010000440         1813 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZOYL010000440 DAZOYL010000000
VERSION     DAZOYL010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.444"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..577
                     /locus_tag="ACHUOY_004072"
                     /pseudo
     CDS             1..577
                     /locus_tag="ACHUOY_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    170..286
                     /locus_tag="ACHUOY_004072"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            579..945
                     /locus_tag="ACHUOY_004073"
                     /pseudo
     CDS             579..945
                     /locus_tag="ACHUOY_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            948..1346
                     /locus_tag="ACHUOY_004074"
                     /pseudo
     CDS             948..1346
                     /locus_tag="ACHUOY_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
BASE COUNT          443 a          451 c          551 g          368 t
ORIGIN      
        1 ccaaagattg gctgtatact ggagactctg cgtgtctggg tacgccagca tgagcgggat
       61 accgggggcg gtgaggtggg ctcaccaccg ctgaacgtca gcgtctgaaa gagccggaac
      121 gtgaaaatcg tgaactgcgc cgcagtaaca atatccttcg cctggcttcc gcttattttg
      181 caaaggcgga gttcgaccgc ctctggaaaa aataatgcca ctgctggata agctgcgtga
      241 gcagtacggg gtcggatcgg tatgcagtga actgcatatt gccccgtcaa cgtattacca
      301 ctgtcagcaa cagcgacatc atcatgataa acgcagtgcc cgtgcgcagc gcgatgactg
      361 gctgaagaaa gagatactgc gcgtatacga tgagaatcat caggtatacg ctgtgcgtaa
      421 agtctggcat cagttgttac gggaaggtat cagagtggcc agatgcactg tggcacgtct
      481 catggcggtt atggggcttg ccggtgttct ccggggtaaa aaggtccaca ctaccgtcag
      541 ccggaaagcc gttgccgcag gcgaccgcgt aaaccgtcat caaggcaata tgccgcgtac
      601 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataaacacac
      661 gtcagcagtt ccgtcagcac tgcgacagcg ttgtccgcaa ggcttttacc cagtccagcg
      721 ttacggtgcc ccacgcctga cggatgaact gtgtgctctg gttaccactt taacgtaaaa
      781 accgtggcag ccagtctgcg ccgtcaggaa ctgagcgcaa aagcctccca gaagttcagc
      841 ccgatcagtt accgtgcaca cggcctgcct gtatcagaaa atctgctgac gcaggatttt
      901 tacgccagtg gcccgaacca gaagtgggcg ggagacatca cgtactatta cagctcaccg
      961 actgcgggga aacacggggc gccgggttat tgaaggtgac ctgtccagct acttcgacac
     1021 agtacatcac cggctgctga tgaaagccgt ctgccgcagg atcagtgatg cacgttttat
     1081 gagactgctg tggaaaacgc cgtgttgatg ttggtctctt ccgggcggtc agtgaaagta
     1141 tgccacaggg cggtgtgatc tcgccgttgc tgtcgaacat catactgaat gagttcgacc
     1201 agtatctgaa taaacgctac ctgagtggaa aagccagaaa ggatcgctgg tactggaatc
     1261 acagtatcca gcgaggccga agtactgctg tcaaagaaaa ctggcaatgg aaacctgccg
     1321 ttgcttactg ttgctatgcc gatgactgtg tgccacgaag gcgcgtcctg gggacgtgag
     1381 tccctgtgca gccatgcggc acaagagatg agggaaggcc cctcggtgtc ggcgtgcagg
     1441 cgcaggcacc aaaccatcca ctgctgctgc atttaagaga tgtggtggtg agcgagatgg
     1501 gaaaaggtcg gacgtgagcc gatcaggtat ggttcgaaga gacgaacgaa agtgaaccgc
     1561 tgatgatgtg tcgaaagcat atagatgatg tcaaaacaat ggtggagtcg ttcctgtggg
     1621 atatgtttac tgcctgcgcg gcatccggca taaaagcggc gtgaagtcga ttcaggctct
     1681 tgtggggaac gtggacctgt caccccgatg tgaagggaga agcgcaagtg gaagccccat
     1741 gagcgccaga gtaccaatgc ggggtacagg gacggagcac caagcgattg cgcagtaaac
     1801 atgcttgaca act
//
LOCUS       DAZOYL010000441         1802 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZOYL010000441 DAZOYL010000000
VERSION     DAZOYL010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.445"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            95..577
                     /gene="tnpA"
                     /locus_tag="ACHUOY_004075"
                     /pseudo
     CDS             95..577
                     /gene="tnpA"
                     /locus_tag="ACHUOY_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            complement(800..1156)
                     /locus_tag="ACHUOY_004076"
     CDS             complement(800..1156)
                     /locus_tag="ACHUOY_004076"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFR0684676.1"
                     /translation="MIQAQEYIGAGYHWVVDLDLEKFFDRINHDVLMSRIEKRVSDKR
                     VLSLIRRFLNAGVMDAGLVRPVTEGTPQGGVISPLLSNLFLHYAFDMWMQRQCPDVPF
                     ERYADDGVPRRRAPGT"
     gene            complement(1217..1420)
                     /locus_tag="ACHUOY_004077"
     CDS             complement(1217..1420)
                     /locus_tag="ACHUOY_004077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684677.1"
                     /translation="MRVSELPDYLRHHWPELKAQLLSGRYRPSPVRRVSILKPGGGER
                     LLGIQMVVDRFIQQAMMQVLQAL"
     gene            1446..1802
                     /locus_tag="ACHUOY_004078"
                     /pseudo
     CDS             1446..1802
                     /locus_tag="ACHUOY_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          376 a          526 c          479 g          421 t
ORIGIN      
        1 cggcacggaa ccacggcagc ttgacggctg caatgacgtg tgtcgtctgg cggtggagta
       61 tctgctgtat gcagcccgaa aacgggggct ggaaatcggc atcttctgca ccatccacac
      121 tttacgcctt cactttgaag aacatttacc cctcgttgta gccggacgtc gcctgggcgt
      181 accaaaatca acagtttgta gtatgtttgt gcgcttccgc aaagctggcc tttcatggcc
      241 tctgcccgca ggtatgtcgg agcgggaact tgacgcccgt ctttacggga gtgcctccac
      301 agtacctgtc gtacttactg aaagtaccgt tatgcccgaa gtccccgggg taaaaaagcg
      361 tccccggcgc cccaacttcc cttatgagtt caaaatcgcc ttagtggagc agtcactgca
      421 gcccggagcc tgtgtggcgc agattgcccg tgaaaatgga atcaacgata acctgctctt
      481 caactggcgc catcaatacc ggaaaggtgg cctgctgcct tccggaaaaa atatgccggc
      541 actgcttccc gtgacgttaa cgccggagcc ggataaccat ggttttgaca tcatctatat
      601 gctttcgaca catcatcagc ggttcacttt cgttcgtctc ttcgatccat acctgatcgg
      661 ctcacgtccg accttttccc atctcgctca ccaccacatc tcttaaatgc agcagcagtg
      721 ggtggtttgg tgcctgcgcc tgcacgccga caccgagggg ccttccctca tctcttgtgc
      781 agcatggctg cacagggact cacgtccctg gtgcgcgcct tcgtggcaca ccatcatcgg
      841 cgtacctttc gaagggaacg tcgggacact gacgttgcat ccacatatca aaggcatagt
      901 gcaggaacag atttgagagc aggggcgaga tcacgccgcc ctgcggcgtt ccttctgtca
      961 ccggccttac cagacctgcg tccattaccc ctgcattcag gaacctgcgg ataagtgaca
     1021 acacccgttt atccgatact cgtttctcta tccggctcat cagcacgtcg tgatttatcc
     1081 ggtcgaagaa cttctccaga tcgaggtcga ccacccagtg atacccggcc ccgatatatt
     1141 cctgggcctg tatcactgcc tggtgggccg aacgaccggg acggaacccg tagctgttgt
     1201 cactgaacga gctatctcac agtgcctgca atacctgcat catagcctgc tggatgaagc
     1261 gatccaccac catctggatg cccagcagac gttcgccgcc gccgggcttc agtatggata
     1321 ctcttctcac aggggatggg cggtatctgc cggacagcag ttgcgctttc agctctggcc
     1381 agtggtgcct caggtagtcc ggcagttcac ttacgcgcat gccatcgact cccgccgcac
     1441 cttgattgcg tcttgtcgat ggaacagcaa tcagcgcgcc cgggggcggc agcgctgaat
     1501 ggcgactaca tatgggatat gatcctcata cctgtcagtt cactgatttt gagctaaccg
     1561 acagcagaga cgctgaacgg ctggatcgat ttgcgcaaac ggcagacgag atacgaattg
     1621 ctgaccgggg attcggttcg cgtcccgaat gtatccgctc acttgctttt ggagaagctg
     1681 attatatcgt ccgggtttac tggcgaggat tgcgctggtt aactgcagaa ggaatgcgct
     1741 ttgacatgat ggattttctg cgcgggctgg attgcggtaa gaacggtgaa accactgtaa
     1801 tg
//
LOCUS       DAZOYL010000442         1786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZOYL010000442 DAZOYL010000000
VERSION     DAZOYL010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.446"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(136..1086)
                     /gene="corA"
                     /locus_tag="ACHUOY_004079"
     CDS             complement(136..1086)
                     /gene="corA"
                     /locus_tag="ACHUOY_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR0684678.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1238..1294)
                     /gene="ysgD"
                     /locus_tag="ACHUOY_004080"
     CDS             complement(1238..1294)
                     /gene="ysgD"
                     /locus_tag="ACHUOY_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR0684679.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1456..>1786
                     /locus_tag="ACHUOY_004081"
     CDS             1456..>1786
                     /locus_tag="ACHUOY_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684680.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          482 a          445 c          438 g          421 t
ORIGIN      
        1 tggtattaac gattttactg agtatcaatt aacacaagaa aataaaatag agcgagtttt
       61 caatccgcat gactttaaat aagacaattt ttagaaataa gccggataaa aaaatagctt
      121 tatccggcac tcatattaca gccagttctt gcgcttaaag tacagatacg gtgccaggcc
      181 cgcgaggatc ataaagataa tcgcgcctgg gtagccgaaa ctccacttca gttctggcat
      241 aaactcaaag ttcatcccat agctggaagc aacgagcgtc ggcggcagga ataccacgga
      301 taccaccgag aagattttga tgatgcggtt ctgctcgatg ttgataaaac ccatcgccgc
      361 ctgcatcagg aagttcacct tctggaacag ggattcgtta tgcggcagca gggattcgat
      421 atctcgcagg atttcacgcg cctgctccag ttgcccaccc ggtaaacgcg ctttacgcac
      481 caggaagttg agcgcgcgct gggtatccat cagacacagg cgaactttcc agccgatatc
      541 ttccagttcc gccagagtgg agagcgcctc gtcgtactca tcgccctgat gcccttccat
      601 aatcacccgg ctcaactgct ccaggtcgct atagatattt tcaatttcat ctgccaactg
      661 ttcgattttg gtttcgaaca gatccagcag cagctcgtag gcgttaccgt ctaccatcga
      721 ctggctacgg gcacgcatac gatacagacg aaaagcgggc agttcacgct cacgcagagt
      781 aaacagacga ccatcacgga tggtaaatgc cacagtggag ttaccggcgt gatcttccgc
      841 atcttcaaag aagaagaagg agtgaatatg caggccgtcg tcgtcttcaa agaaacgtgc
      901 cgatgcttcg atgtcttcca gttccgggcg ggttgccagg ctctggccaa gttcagattg
      961 tacgcgcagt cgctcgtcgt cgtccggttc gacaagatca atccatactg catttacaag
     1021 gggttgtgac tcttcgactt ccagccgggt cagtcggtta ttttccagtt gaaatgcgct
     1081 cagcatgacc gggactccca atgcttaaaa tatcggacag ttcggtgggc acacagaaac
     1141 aaattgggtt tcagaccatt aaacagcctg actcagcgcg acgggaaaaa ttgaggtcgc
     1201 tgacaaccgc taaggctatc agcaaaaagg gatagcctta ggagttgatc ctggatgaca
     1261 ggatagtgag ccagtatcta ctgggtgtgt ccaaggcgaa tgtcctctta gagtgatcgt
     1321 gcgcgcatgt tacgccagca aaattttgcc gtcaacacgc aacagaacac cacagaacaa
     1381 tagttgaact tcgcgctttt tggtactcgt caaggtcgct aacaggataa aaaaatccaa
     1441 catccaggac ctataatggc gcatcggcta ctcattggta aaggaatgat caccttgaat
     1501 ctcaaacgga tttttctcgc cctcacctta cttcccttat ttgctgttgc cgctgatgat
     1561 tgcgcactct cagatccgat gctgaccgta caggcgtata ccgttaatcc tcaaacagag
     1621 cgggtgaaaa tgtactggca aaaagccaat ggcgaagcgt ggggaacttt acatgctctg
     1681 ctggtggata tgaatagtca gggtcaggtg cagatggcga tgaacggcgg catctatgat
     1741 gaaagctatg cgccgctcgg tttgtacatc gaaaacggtc agcaga
//
LOCUS       DAZOYL010000443         1782 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZOYL010000443 DAZOYL010000000
VERSION     DAZOYL010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.447"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>1782)
                     /gene="traD"
                     /locus_tag="ACHUOY_004082"
     CDS             complement(<1..>1782)
                     /gene="traD"
                     /locus_tag="ACHUOY_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR0684681.1"
                     /translation="FSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTT
                     LEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVIC
                     LITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARMLKKDGRDSDIRIGDLPIIR
                     DSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSIDKILN
                     PLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYLMRNDP
                     NRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKAIRYLQ
                     GIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMG
                     ENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAAT
                     LFDVMNTRAFFRSPSHKIAEFAVGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQ
                     TLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQERPKVAPEFIPRDINPEMENRLSA
                     VLAAREAEGRQMASLFEPDVPEVVSGE"
BASE COUNT          448 a          493 c          429 g          412 t
ORIGIN      
        1 cttctccgga aacaacctcc gggacatccg gttcgaagag gctggccatc tgacgacctt
       61 ctgcttccct tgcggcaagt acggcactca gacggttctc catttccggg ttgatgtcac
      121 gcggaataaa ctccggagcg accttcggac gttcctgata tttcagtgag agtttgactg
      181 ccggatacgg tccgggcagg gtgacatagc aggtcagatc cggcagggac tgaatgtcgg
      241 aataactgac cagcgtctgg cgctccatat ccttaccggt cgataccccg tcacgtaccg
      301 gatcagcacc gtaggaatac tgttcgctgg ctttcaggtg ctctttctca ccaatttcac
      361 cgacagcgaa ctccgcaatc ttatggctgg gagaacggaa aaaggcacgg gtgttcatga
      421 cgtcaaacag cgtggcagcc gctttctcac cgtagatatc ttccagctgg gcataggact
      481 ggataccaaa cacataacag ccaccgaact tacgggcttc cggcaggatc tccaccaggt
      541 ccggcagttt gtgtaatgtg ggtaactcat cacagaagaa ccacacacga cgattacggt
      601 tttctcccat tgccagcaga ccacgaatgg caatggacag ccacatggag atcaccggct
      661 tcagggaggc atgagtgtcg gcattcgacg agataaacag ccagccgttt ttctgatcct
      721 cccggacacc acgcatccag tcacggatgg tgaagggctc accgttatgc tcaatcccct
      781 gcaggtaacg gatggctttc acatagttgg tcagcacagc acggatggaa atcgccgttt
      841 tctcaatttt ctcttccacc aggttggccg ccggtgaatt acgcaggtag gtacgcagtt
      901 tttcaatttt gatggaaagc agtgtgtcca ccagtttgct gtagctgcgg ttggggtcat
      961 tacgcatcag atacgccgct tccgcaaaaa tggtacgtcc tgaaccctgc cagaacgggt
     1021 cctcttttgt gcccatcggg atcagagtat ttgcggtatt atcaaaatcc ggctgtgtca
     1081 ggcactcctt ccacaaatcc caggcggcac agcgtgcatc cagcggattc aggatcttat
     1141 cgatggaggg atcatagtaa cttttaacaa attcccctga acggtcataa atcaccacca
     1201 tatcaccacg ctgacgggcg tagttggcca gacgacggat aacctccgac ttaccggccc
     1261 cgaccgtgcc gtgcagacag aagttctgga tttcggaatc ccggataatc ggcagatcgc
     1321 caatccggat atcggagtcc ctgccgtctt ttttcagcat ccgggcaacg tctttcggat
     1381 tgtctgtcag ttgacgacca cctgtgactt cattttcgct ctgttgttta ccctgacgcc
     1441 ccagaatcca ggagacaaca aagaaggtaa tcaggcaaat aaccagtgcc acaactgtgg
     1501 ccagaacgaa tgcagaccat aactgctcac cgcaccagat catatattta tcatgcagta
     1561 cctgagcagc gttcatccgg aaggttttgc cgtaatactg aatctcatat accggctgag
     1621 acttgattaa atcccgcatg ccttccagcg tggtacacca ccagtaaata cagccattca
     1681 caaacgtctg ccagcttatt tttacccata aaaccagacc aacgagtatc cagaaaaaaa
     1741 taaacaggca gtaaagcata atattggcga tctggctgaa ca
//
LOCUS       DAZOYL010000444         1782 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZOYL010000444 DAZOYL010000000
VERSION     DAZOYL010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.448"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(80..1105)
                     /locus_tag="ACHUOY_004083"
     CDS             complement(80..1105)
                     /locus_tag="ACHUOY_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HFR0684682.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            complement(1131..>1782)
                     /gene="rfaY"
                     /locus_tag="ACHUOY_004084"
     CDS             complement(1131..>1782)
                     /gene="rfaY"
                     /locus_tag="ACHUOY_004084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR0684683.1"
                     /translation="KDNDNKYKEIFDEILAYNFKTVKVLRNINDTKVSLIDTKYCRYV
                     FKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYAS
                     VFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVR
                     IIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          559 a          358 c          285 g          580 t
ORIGIN      
        1 tctcttgcct gaactaataa aatatgatag tactcatcct taattattat tgtaactcag
       61 acatccatga tctttattat tatccaaata gttttctttt atacttattt aatttaaatt
      121 taataaagta aataaaagcg ttaaacgtat caccattttt ctttgcatgt tgatatttca
      181 cttgatactg cttttcattc gtggctggaa taaacgccac atcattccag caagaagcct
      241 gataagcctc attaaaatac ttctggcagg gataattcgt ccagtcgttc cagggcttcg
      301 ttacgccaac ataatgaatc agcttagtat catcggtaat ggtcttttta taatcttcat
      361 cgcttttatt ttttaactca taatcgatgc cataaaagca atcataatcg ccgctaataa
      421 aaatacaatg accaaagaac agaatattta aaatatcctg atcaaggcat ttatacttcg
      481 tgtgctgcgg gtcacttgcc agcatagcaa tggctttatt cataaaatca tttttcgccc
      541 atgcttcaag ctgcagatat actacaccgg cgttgaaata attccctgga agtccctcta
      601 tagccagacg cttcgctggt ttttcctgca taaaaggcac atctttaata acagctgcaa
      661 acttatcccc atcaaaatta atatcaagca atccagctaa tgagccttta cagatgacat
      721 cagcatcgat atataacaga ttttcaatag tttcactcag atactggaac gcaactaaac
      781 ggaagaatgt tgcataagac cactgtttca ggctggggaa gatatcaata aaggcactgg
      841 agactttata aattcttact tcaacgttag catttttgct ggtaaaagca ttaacacgtt
      901 gtagataatc ttcatcaatg tagtcggtaa agagatgaaa cttaaccgca aggtccggat
      961 tatgcataac aactgattgc atagatactg ccgcaccaaa taaaaagtta cggtcaatac
     1021 cgtaagcgat atttaacaca cgatcggtgt tagcaatttc cccagagaca gcgacttcag
     1081 taatactctc agctaataaa tccatgttgg ttccgttttg actgtgtggt ttacgctttg
     1141 ccttttaatt ttttaataaa tttgcgtagt ttggttctgt agattactga atagtagcca
     1201 taatctttta tttcgttggc aatacccagg tgacgttcca tggccagacg atcacgagct
     1261 ttacgctctg ccgtacagct tttaccggat aggtcaatta tcctgacacc atctttacta
     1321 acgataaagt taccccggtg tgggtcgccc gatagcatat ttaaggcatg caatttttcc
     1381 attgaagcct taatttctgc tttaacatct tctggaatga tcggcatatc attgagctct
     1441 acgccctcga catactcgat aagcatgata aaaacactgg catagttgaa aattttacgc
     1501 tcagccagaa aataaaaatc attaggaaaa gtaagacctg cacttctgac acgatcggtt
     1561 tcaacgatca aattctgata gtagtcacct tttacaaaag attttaaaaa acgttcattc
     1621 cttttcgttt tcggggcaaa gactttaaaa acatagcgac aatatttcgt atcaatcaat
     1681 gatactttgg tatcattaat attacgtaat acttttactg ttttaaaatt ataggccagt
     1741 atttcatcaa agatttcttt atatttatta tcattatctt tg
//
LOCUS       DAZOYL010000445         1777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZOYL010000445 DAZOYL010000000
VERSION     DAZOYL010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.449"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..467
                     /locus_tag="ACHUOY_004085"
     CDS             <1..467
                     /locus_tag="ACHUOY_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0684684.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            482..1084
                     /gene="yadL"
                     /locus_tag="ACHUOY_004086"
     CDS             482..1084
                     /gene="yadL"
                     /locus_tag="ACHUOY_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR0684685.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            1111..1707
                     /locus_tag="ACHUOY_004087"
     CDS             1111..1707
                     /locus_tag="ACHUOY_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0684686.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
BASE COUNT          549 a          373 c          390 g          465 t
ORIGIN      
        1 aagcgacaac ctgtaacatc acacttactg gtaataacgt cacgaatgat ggcaataata
       61 actacacatt gagaatccct aagatgggtc tggataagat cgcgaataaa acgacagaat
      121 ctcaggctga ttttaaactg gttgctagtg ggtgcagcag tggcatcagt tggattgata
      181 ccactctgac cggaaatgca tcatcaagct cacctaagct tattataccg cagtctggtg
      241 attcatcttc gacgacaagt aatatcggta tgggtttcaa aaaacggact actgatgatg
      301 ccactttcct taaacctaac agtgcggaaa agatacgctg gagcacagac gagatgcagc
      361 ccgataaggg tcttgaaatg accgttgcgc tacgtgaaac agatgcaggg caaggcgtac
      421 cggggaattt tcgtgcactg gcaacgttta atttcatcta tcaataagaa ggataattaa
      481 gatgacattt aaaaatttac gttatggatt atccagcagt gtcgttttgg ctgcctcatt
      541 gttcagcgta ccctcttatg cggcaacaga cagcattggg ctgaccgtga ttactaccgt
      601 agaaataggt acttgtaccg ctacattagt aaatgactct gatcaggaca tttctgttgt
      661 tgactttggc gatgtatata tttctgaaat caatgccaag accaaagtta aaacattcaa
      721 actcaaattc aaagactgtg cgggtatccc caataaaaaa gcacaaataa aattaaccaa
      781 gcgagccaca tgcgaaggta ctgctaatga cggtgcgggg ctcgcaaatg gatccacagc
      841 cgcagataaa gcaagtgccg tcgccgttga agtctggagc accgaaactc cggcaacagg
      901 gagcgcaaca caatttagtt gtgtgacacc agcatctcaa gaggtcacta tctccaatgc
      961 agccaacgcg gttgtttatt atccgatgag tgctcgactg gtcgtcgaaa aaaataaaac
     1021 cgtaagcaat gtcactgcgg gtaaattttc tgcacctgcc acatttacag taacctataa
     1081 ctaacagact gacagatcca ggaactatat atgcatccca ctcaacgtaa gctgatgaag
     1141 agaataattc tgtttctgtc actacagttt cccattacct atccagccat cgctgggcag
     1201 gatattgacc ttgttgccaa tgtaaaaaac agcacttgca aaagcggaat cagtaaccag
     1261 ggtaatattg atcttggcgt cgttggggtg ggatattttt cagataatgt tactcctgaa
     1321 agctatcaac caggtggaaa agagttcact gtcacagtat cagactgtgc attacaggga
     1381 actggcgatg tgcttaatca gttacatatt gattttagag cccttagcgg tgtcatggct
     1441 gcaggttcaa ggcaaatatt tgccaatgaa gtcgcaaccg gggctaaaaa tgtcggtgtc
     1501 gttctctttt ccattcagga cccgaccaat atattcaatg ttattagttc agcaggaaat
     1561 tctcgttccg tgtatccagt aatgacttct gccctgcata attcttcatg gaaattttat
     1621 gccagaatgc aaaaaatcga tcccgcgctg gatgtcatat ctggccaagt aatgagtcat
     1681 atattagttg atgtgtacta cgaataatag atcaaaacaa ataactgcga ctgttacagc
     1741 agtagatggt ggagtgataa tgaagaaatt cttcaga
//
LOCUS       DAZOYL010000446         1762 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZOYL010000446 DAZOYL010000000
VERSION     DAZOYL010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.450"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            79..1554
                     /locus_tag="ACHUOY_004088"
     CDS             79..1554
                     /locus_tag="ACHUOY_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR0684687.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT          498 a          363 c          319 g          582 t
ORIGIN      
        1 acattagctt acaatcgctt taaatatgac agcataacct ttacataatt tagttccaga
       61 aaacaatcat tcggaaaaat gattcagtca acacgtattt ccatggggtt attctttaaa
      121 tattttttat cgttaacgaa aattgatcct ggtcaaaact atatatctct gccatcaata
      181 aaatccagca ctcacattgc tctccttttt atggtttcta tgggtacaca aaaattaaaa
      241 gctcaaagct tttttatttt cagtttattg ctgacgttaa ttttattttg cattactacc
      301 ttatataacg aaaacacaaa tgtaaaactc atcccacaga tgaattacct gatggttgtt
      361 gtggctttgt ttttccttaa cgccgtcatt tttcttttca tgttaatgaa atatttcact
      421 aacaaacaaa ttttaccaac actcatttta agccttgcat ttttaagtgg ccttatctat
      481 ttagttgaaa ccattgtaat tatccataaa ccaattaacg gcagtacact gatccagaca
      541 aagtcgaatg atgtttctat tttctatatt ttccgccaac tcagttttat ttgtttaacc
      601 tcgctggcac tcttttgtta tggaaaagac aacatccttg acaacaataa gaaaaaaacg
      661 ggaatcctgt tgctggcgct gattcctttt ttagtttttc cccttctggc acacaatctg
      721 agcagttata acgctgacta ttctttgtat gtcgtcgact actgtccgga caaccatact
      781 gcgacctggg gaatcaacta tacaaaaata ttggtttgtc tgtgggcatt tttactgttc
      841 tttattatca tgcgtacacg attagccagc gaactatggc cgttaatagc attattatgt
      901 ctggcatcgc tatgctgcaa cttacttcta ctgactctgg atgagtataa ttacaccatc
      961 tggtatatca gtcgcgggat tgaagtttcc agtaaactgt ttgttgtgtc ttttctgatt
     1021 tataacattt ttcagaaact gcaactctcc agcaaactgg cagttcatga tgtgctgacc
     1081 aatatttata atcggcgcta ctttttcaac agcgtagagt cattattgtc gagacctgtt
     1141 gttaaggact tctgtgtcat gctggttgat attaatcagt tcaaacgcat caatgcccaa
     1201 tggggacatc gtgtgggtga taaagtgctg gtttcaattg tcgatattat ccagcaaagc
     1261 atccgccccg atgatatttt agcgcgactg gagggtgagg tgtttggctt gctatttacc
     1321 gaactcaata gtgcccaggc aaaaatcatt gcggaacgta tgcgtaaaaa tgtcgaactc
     1381 ctgaccggct ttagtaacag atatgatgtt cctgaacaaa tgaccatcag tattggcacg
     1441 gttttttcaa cgggtgacac gcgtaatatc tcgcttgtca tgacggaagc agataaagcc
     1501 ttacgcgaag cgaaaagcga ggggggcaac aaagtgatta ttcatcatat ttaagtgcaa
     1561 aaatattcag agccatgctt tttgcgtggc ttttgcatac aatttattac tattacccct
     1621 aaatttcccc tccccagcgg cgcagagatg agtataatta gcgcccctgt gccaggccgc
     1681 aatcgaactt tatctggttt tctcgtttca ctgaaccgaa ggagtgccat ttatcatgaa
     1741 attgcaccat agaatgctcc gg
//
LOCUS       DAZOYL010000447         1759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZOYL010000447 DAZOYL010000000
VERSION     DAZOYL010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.451"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..74
                     /locus_tag="ACHUOY_004089"
     CDS             <1..74
                     /locus_tag="ACHUOY_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0684688.1"
                     /translation="RLKGKLTPALLQILIREIKGSSH"
     gene            74..421
                     /gene="tnpB"
                     /locus_tag="ACHUOY_004090"
     CDS             74..421
                     /gene="tnpB"
                     /locus_tag="ACHUOY_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0684689.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            441..1595
                     /locus_tag="ACHUOY_004091"
                     /pseudo
     CDS             441..1595
                     /locus_tag="ACHUOY_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1599..1759
                     /locus_tag="ACHUOY_004092"
                     /pseudo
     CDS             1599..1759
                     /locus_tag="ACHUOY_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          420 a          472 c          501 g          366 t
ORIGIN      
        1 tcaggcttaa aggtaaactg actccggcgt tattacagat acttatccgc gaaataaaag
       61 ggagcagcca ctgatgatat ctttccctgc aggttcgcgt atctggctgg ttgcaggtat
      121 caccgatatg cgaaatggct ttaacggcct ggcctcaaaa gttcagaacg tcctgaagga
      181 tgacccgttc tccgggcatc tgttcatctt ccgcggacgc cggggtgacc agataaaagt
      241 gttgtgggct gacagtgacg gactgtgcct ctttaccaga cgcctggagc ggggccgctt
      301 cgtctggccg gtcacccgtg acggcaaggt gcaccttact ccggcccagt tatccatgct
      361 tcttgagggc atcgactgga aacacccgaa acgaacggaa cgcgctggca tccgcatata
      421 acccgttgta aagtgaggat atggacacct cacttgctca tgagaatgcc cgcctgcggg
      481 cactgttgca gacgcaacag gacaccatcc gccagatggc gaaatacaac cgcctgctct
      541 cacagcgggt ggcggcttat gcttccgaaa tcaaccggct gaaggcgctg gttgcgaaac
      601 tgcagcgcat gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga
      661 tacaggaagc acaggagcga atcagcgcac ttcaggaaga aatggcggaa acgctgggtg
      721 agcaatatga cccggtactg ccatctcccc tgcgccagtc ttcagcccat aaaccgttac
      781 ctgcctcact tccccgtgaa acccgggtca tccgtccgga agaggaatgc tgcccggcct
      841 gtggtggcga actcagttct ctgggatgtg atgtgttaga gcagctggag cttatcagca
      901 gcgcctttaa ggttatcgaa acacaacgtc cgaaactggc ctgttgccgg tgtgaccata
      961 tcgtgcaggc accggtgcct tcaaaaccca ttgcacgcag ttatgccgga gcggggcttc
     1021 tggcccatgt tgtcgctgga aaatatgcag actatctgcc gttataccgc cagtcagaaa
     1081 tataccgtcg tcagggcgtg gagctgagcc gtgccacact ggggcgctgg acaggtgctg
     1141 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
     1201 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
     1261 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa atgcctccgg
     1321 cggtctggtt cgcgtactca ccggaccgga aaggtatcca tccacagaat catctggccg
     1381 gttacagcgg agtgcttcag gccgatgctt acggtagtta ccgggcgttg tacgaatccg
     1441 gcagaataac ggaagcccag cagcgtatcg gtgaactgta tgccatcgag gcagaagtcc
     1501 ggggatgttc agcagaacag cgtctggcgg caagaaaagc cagagccgcg ccactgatgc
     1561 agtcactgta tgactggata cagcaacaga tgaaaattca ttcgctgaaa atggaatgtc
     1621 tccacggaga acactattat ccatcgggaa atagtgcggg caacagtgtt tagttatatc
     1681 gagtgcgatt acaatcgctg gcgacggcac cgttggtgtg gtggtctcag tccggaacaa
     1741 tttgaaaacc agagcctca
//
LOCUS       DAZOYL010000448         1749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZOYL010000448 DAZOYL010000000
VERSION     DAZOYL010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.452"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(186..296)
                     /locus_tag="ACHUOY_004093"
     CDS             complement(186..296)
                     /locus_tag="ACHUOY_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684690.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            complement(349..753)
                     /locus_tag="ACHUOY_004094"
     CDS             complement(349..753)
                     /locus_tag="ACHUOY_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR0684691.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(974..1705)
                     /gene="bluR"
                     /locus_tag="ACHUOY_004095"
     CDS             complement(974..1705)
                     /gene="bluR"
                     /locus_tag="ACHUOY_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR0684692.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          481 a          367 c          369 g          532 t
ORIGIN      
        1 gaataaggtt tcaatttttg agttatatag gaaaggttta acctgttcct ggctaaaata
       61 catataactg gatgatgact aaaccaaaac acatgtgcgt taagtattga aacgggcgta
      121 tggcacaccc gttgttttta taaatatatt aaccgttata aaataacgta tagaaagtca
      181 agtgatcaca tttcaaatat caatttgata gtattggcct ggtgattatt tatgggtagc
      241 aataaaagga cagtatttat catccatagg gatagtctct gtacttttat tcccattatg
      301 ctaatgcctt actgaattat gaagcatttc ttaagcatcc aactttagct agattaatgg
      361 tttattattt tctacatctt caatatataa aagcgtatta tcaatggcgt agtaactgcg
      421 tttgttatga ttaacatcag taacccaacg gaaaacgccc gcgcctgcca gtgttgaaca
      481 gtattcccga aatgtagatt ttccgtaaat atgaagcaat gcggcctctt ttattttagc
      541 agggttcttg gtcgtactaa cttttaacag gttcctggtt cctcttaata acaaaaccgt
      601 gtcatcgtga gtaataattc tgatgttatc cgtagccaga taataaatgt aatgtgcaat
      661 acggtgatgt tttaattctg aataaaacca ggagaagttt tgctcttttc tcacttgctc
      721 aaacatcttt tgaaaaacaa cgacctgatc catcaggata ataacctctt gttagttgtg
      781 agactgcgta gtgtgcacga tcggttttac cacttcaatc tggtctgtcc tttggctgtg
      841 atatgtacag agtgtgatag agggattacc tgaattcttc cggtgagcat tttgcaacgg
      901 accagctccg gtacaaatgc tgttgtgggt tcagattata acattctgtc taaggggcgg
      961 gataaaggtg aaattagggg gcatgaaaga tgactttata accttgctca ccccaatgtt
     1021 gtaaaagttc gttttgcctt ctcgttggtg ccatgcctgt ccagacaatc aatgtttgcg
     1081 tcgggaacag ttccgggtgc ggcgattcaa tgggatcagc aagaacagaa atgtgccatc
     1141 ctgacaatga taaacgccat gcttccagcc acaatcgtgc tctctcttca acattccacg
     1201 ccatcagaat ggcttcttta ccgggcttac ggcgcatttc gaaaagtgag gctgctgcat
     1261 attcaattaa tgcgccgtca aacatactgc tcataatgcg ggaggtgttg tgatcaagca
     1321 cgagacgctg gcgaacagga aggtaaacat gattaatcaa ttgatctact gggtactctc
     1381 gacccagtga aataattctc gcgcgtagtt tggcaggatt agccatacga agaattgaca
     1441 tcatctcttc ttgcaggcgg ttccagtcat cttccgtatc ctggctggtg gtttccagta
     1501 atgctttaac tttgcctaca gggacgccat tacttatcca acgcttgatc tcttcgatgc
     1561 gttgtatgtc ttcttcatca aagagtcggt gtccgccttc actgcgctgt ggttttaaca
     1621 aaccgtagcg gcgttgccag gcccggagag tgacaggatt aatcccgcaa cgttcagcaa
     1681 catcaccaat gctgtaataa gccacaattc ctccttgcgg tcataaatct ccgccgcctg
     1741 tacacgacc
//
LOCUS       DAZOYL010000449         1748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZOYL010000449 DAZOYL010000000
VERSION     DAZOYL010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.453"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..416)
                     /locus_tag="ACHUOY_004096"
     CDS             complement(<1..416)
                     /locus_tag="ACHUOY_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0684693.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
     gene            complement(432..1106)
                     /locus_tag="ACHUOY_004097"
     CDS             complement(432..1106)
                     /locus_tag="ACHUOY_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0684694.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(1186..1728)
                     /locus_tag="ACHUOY_004098"
     CDS             complement(1186..1728)
                     /locus_tag="ACHUOY_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0684695.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          498 a          389 c          313 g          548 t
ORIGIN      
        1 ctttccagtt cttccaccca ggcctgcggg acactgaaat cgagacgaaa aaccccgata
       61 tcccaggtat agctcccacc ctgaacaagt tgctcaaatg ttaaacattg cttaccgctg
      121 gcgaagttat cgctattaat gcctaaccgc ttgataactt ctcttgataa acatgtttct
      181 tgcgggttgt ctttaacaat aatctcatat ttcccgcgcc attgcttatt gacataaata
      241 tcgatgtcat actgccccgg taagggttga ttatcatcaa gacgaatatt tgcaacctgc
      301 tggtctttca ttccacctat cataaaatgg gtatcaaagg tttcttcagc tgcataagct
      361 tcaatgccga gcaataacgc tacgattgct gatgcaagtg gggtcattct caacataggc
      421 agctcctgca attaaatttt gtcactaata tagttgccat ggtcatcgat aatggtcaga
      481 tgccagttat ttgcattatt acttttgaca ttaacactct gactttctaa gggggcaatc
      541 ataatagttt cataattgac tttgacatta ttagctttga catccgaaat cgtcacccaa
      601 ttagctgagt cattttttat caccaaacca ttgccactgc gatttaccag caatttttta
      661 aatgtcgctt tatttaccgg agcaataccc gctggtcggt aaaacaactt aattctgttt
      721 tgcatcgcaa acttcagtgc attcttacct tcttgctctg gactatttgg tggaatatcc
      781 agaacattca gataaaaaat gctttcttta ttagtgggca gtttattcgg cataattttg
      841 atttttactt gctgcccgga atttgcccct atttttgcca ctggtggcgt taacatgaaa
      901 ggaacctgaa ttttttctgg tggtaatgac gtatcgccat catcaatcca cgcctgcagc
      961 agcgaagaac ggtttccctg gttcatcaac tgcaccatca cttctttatt ttctgccggg
     1021 taaataatgc gcgtcccgta gataacaatt ccggcatgtg caggaaggac catagaaaaa
     1081 atgagtaaag ataataatcc tttcatttta atgcctctta aattaaaagg ccgccatggg
     1141 atagcggcca tgtttattac ctggagaaac ttacaatttt atttattaat cctgcgccac
     1201 acgcataacc agagaagata acacatcacc tgattgcact gagttatctg tcagtttcag
     1261 gtattttgct ttaaacggaa tagcatattg accttccgct gttttataac tctcaatgtt
     1321 aaaaggtgca gaaacatcca ggacatcgct gttatcacct aaatagagat agtaacccac
     1381 ggtatcgttg ctactggcta tcgccttatc gttaccggtt attttatcgt tggtaatcaa
     1441 cgagagtttt acaccagcaa cttctttcgg gcagctaatt tttaacatgc tgctggttac
     1501 accgactgca tcatcatagg tagaatattt agctttacct tcaaagacag tggttggtac
     1561 agtcggcata ttaatactta ctgaatcacc acccgcttcg aatttacacg gagattctgc
     1621 aatattcccc ttaatagtta atgttccggt atcaacgtct gcagcaaata ctccagcgct
     1681 cataaaaaaa gaagaaaaga cagcggcagc aataattgaa cgtttcataa tatgttcctt
     1741 aataaaat
//
LOCUS       DAZOYL010000450         1742 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZOYL010000450 DAZOYL010000000
VERSION     DAZOYL010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.454"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(23..1639)
                     /locus_tag="ACHUOY_004099"
     CDS             complement(23..1639)
                     /locus_tag="ACHUOY_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFR0684696.1"
                     /translation="MRKNNKTSVLLIIIILAMILGVVKIQVWLNDWNNDFFNALSQKE
                     TDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIRWREWLTDYYLNRWFADKNYYFTQ
                     IYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQSLSMLITFTVILWQSAGTLSFTV
                     GGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRPLNVEKQRSEATFRTNLVQHNKQT
                     ELIALSNAESLQRQELRDNFHTIKENWHRLMNRQRWLDYWQNIYSRSLSVLPYFLLLP
                     QFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPT
                     NKPKNCQYAVQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSH
                     CWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKL
                     AARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVRE
                     KLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT          507 a          373 c          372 g          490 t
ORIGIN      
        1 tacagcaggg cttttattgt tgttataaaa ccgtgctaat gtcacaaata tcatcagcca
       61 ggttccagac accgggttga tgtgtaacca taatgacgcc gcttgtgggt agtttttcac
      121 gcactaaacg cagtaagcgt atagcctctt gttcctcaag atgagaggta gtttcgtcaa
      181 gaaatatcca tttcggacgt cgtaaaatta atcgtgccag ggcgatacgt tgtttttcgc
      241 cgctggaaag aatatctccc cagcgatcgt ggtcatgaat acgcgcagcc aatttcccca
      301 gaccaacctg atgcagtact tcgctcaacg atttatcgtc tacgggcagg ggaagtgctt
      361 tacaaataat ctctttcagt aagccgcttt tgattaacgg tgtttgtgac acataccaac
      421 tgtcagcagg agaagaaata tcacctttaa accacggcca gcagtgggat aatgttttaa
      481 gcagtgtggt ttttcccgca ccagagtagc ctttcagtaa tagccatttg cctggcgaaa
      541 catgaaagtt caagttctct aatatgatct tattatcagg cgtacgaata ctcgcattag
      601 ccacttgtac cgcatattgg caatttttag gcttattcgt agggcgctgt tcagtgagtt
      661 gatggaactc atacaagcga tcgataaccg cagccagttc agcaagttcg tcatatttat
      721 aaataaacca gcttaaattg ttcgatacta gcataaatgc ctggcgcgat ttcatcagtc
      781 cgcccagatt gatctgcccg ctaataaact gcggcaataa cagaaagtag ggaagaacgc
      841 taagcgagcg cgaataaata ttttgccagt aatcaagcca ccgctgacga ttcattaaac
      901 gatgccagtt ctctttaatg gtatgaaaat tgtcgcgcaa ctcctggcgt tgtagagact
      961 cagcattgct tagcgcaatc agttctgtct gcttattatg ctgcacaaga ttagttcgaa
     1021 atgtcgcttc gctacgttgt ttttccacat taagcggacg aatacgttta cccaccttat
     1081 gggtaaataa agttccaccg atcacaatga gcacgacggt atagaccata tacccctgga
     1141 tattccattc tgttccacca acagtgaatg agagcgtacc cgcgctttgc caaagaataa
     1201 cggtaaaggt gatcagcatg ctaagtgact ggatgaagcc aaatgaaaga ctgagcgttt
     1261 tgctgatcag taagagaatg tcttcagcga tacgttggtc agggttatct gtatttttat
     1321 gctcgccgta gatctgcgtg aagtagtagt ttttatctgc aaaccagcga ttaaggtagt
     1381 aatctgttaa ccattcgcgc cagcgaatag tcagtagttt aattaaccat gttttgttta
     1441 cggatattaa tacaaagatc cccagcaggg cagggaacca aagaacaagc tgccaaagct
     1501 tatcggtttc tttctggctt agcgcattga aaaaatcatt attccagtcg tttaaccaga
     1561 cctgaatttt aaccacgcca aggatcattg cgaggataat aatgattaac aacactgaag
     1621 ttttattatt cttacgcagc caaaagggct tcagcagaca gagatacttt gctatcaaca
     1681 tataaaacgt aatgggaatg gttatcatta gcgaaaattg attgtgccat ccaatgatta
     1741 tc
//
LOCUS       DAZOYL010000451         1741 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZOYL010000451 DAZOYL010000000
VERSION     DAZOYL010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.455"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            182..436
                     /gene="bssS"
                     /locus_tag="ACHUOY_004100"
     CDS             182..436
                     /gene="bssS"
                     /locus_tag="ACHUOY_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR0684697.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            551..1669
                     /gene="solA"
                     /locus_tag="ACHUOY_004101"
     CDS             551..1669
                     /gene="solA"
                     /locus_tag="ACHUOY_004101"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR0684698.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT          459 a          423 c          421 g          438 t
ORIGIN      
        1 aaaaaatatg aatgacatgc tgttttattg ttcatataaa tcttaaaagc agaaatttat
       61 ctgttccccc ctttaaaaat tcggtgaata cccttactta ttggtatact gaagttgctt
      121 cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt agggggccat
      181 gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg acatcagcac
      241 cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa ataagtccga
      301 gcaggaaggg actgaagttg gtcaaacgct ctggttaacc actgatgttg ccagacaatt
      361 tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc aggtaaacga
      421 gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac cggtgccttt
      481 accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc atgagagcag
      541 agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag gcgctgccgc
      601 cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc atatgccacc
      661 gcatcaacac ggcagccacc acggcgatac gcgattaatt cgccatgctt atggtgaagg
      721 cgaaaagtat gtcccgctgg tcctccgcgc gcaaacgctg tgggatgaac tttcccgcca
      781 caacgaagat gatcccattt ttgtacgctc tggtgtcatt aacctcggcc cggctgactc
      841 cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg ttgaaaagct
      901 cgatgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca actacatcgg
      961 cttatttgag actgattccg gttttttgcg cagcgaactg gcgattaaaa cctggatcca
     1021 actggcgaag gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg caattcgtca
     1081 tgacgatgat ggcgtaacta ttgaaacggt tgacggtgag tatcaggcga aaaaagcgat
     1141 tgtctgcgcg ggaacatggg taaaagacct gctcccggag ctgcctgtcc agcccgtacg
     1201 taaagtattt gcctggtatc aggccgatgg ccgctatagc gtgaagaata aattcccggc
     1261 gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag aaaacgacgc
     1321 gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg aacgtgttcc
     1381 gtttgcggaa gtggtcagcg atggttcgga agccttcccg ttcttgcgca atgtattgcc
     1441 gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc ctgacgaaga
     1501 ttttattatc gataccctac ccggccacga taatacactg ctcattaccg gcctgagtgg
     1561 gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg cgcaagacaa
     1621 aaaaagcgat tttgatttga cgccattcag gctttcccgc ttccaataat catcattacg
     1681 gcctccagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt gcactatctc
     1741 a
//
LOCUS       DAZOYL010000452         1736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZOYL010000452 DAZOYL010000000
VERSION     DAZOYL010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.456"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            203..685
                     /gene="smpB"
                     /locus_tag="ACHUOY_004102"
     CDS             203..685
                     /gene="smpB"
                     /locus_tag="ACHUOY_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR0684699.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            900..1262
                     /gene="ssrA"
                     /locus_tag="ACHUOY_004103"
     tmRNA           900..1262
                     /gene="ssrA"
                     /locus_tag="ACHUOY_004103"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1461..>1736)
                     /locus_tag="ACHUOY_004104"
     CDS             complement(1461..>1736)
                     /locus_tag="ACHUOY_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0684700.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          488 a          455 c          401 g          392 t
ORIGIN      
        1 ggtaccagtg cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca
       61 aataatatca tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt
      121 tcatgctaag atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga
      181 ttaccgatga ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac
      241 catcgcgctt aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg
      301 acttgccctg caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga
      361 cagctacgtc cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat
      421 ggccgtggcc tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa
      481 ccagcgcgaa ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc
      541 gctctccctg tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa
      601 aaaacagcac gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg
      661 tatcatgaaa aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc
      721 gcctccctct ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt
      781 tgccatccca ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga
      841 taacgccatt gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg
      901 gggctgattc tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg
      961 gcctcgtaaa aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta
     1021 ataacctgct tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg
     1081 tcaaacccaa aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc
     1141 aggctagttt gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta
     1201 agcatgtagt accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac
     1261 cacttttgac aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga
     1321 ccagacagta agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc
     1381 aaaaaagcct ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt
     1441 aacacgatgc agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc
     1501 agaccactca gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg
     1561 acctgcgcct gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc
     1621 tgctgattat cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc
     1681 atgactaccg gggacaggcg gcccagtgag tcggggccag cattccagat acgacc
//
LOCUS       DAZOYL010000453         1735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZOYL010000453 DAZOYL010000000
VERSION     DAZOYL010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.457"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1713
                     /locus_tag="ACHUOY_004105"
     rRNA            <1..1713
                     /locus_tag="ACHUOY_004105"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          434 a          383 c          551 g          367 t
ORIGIN      
        1 ggagcgttct gtaagcctgc gaaggtgtgc tgtgaggcat gctggaggta tcagaagtgc
       61 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
      121 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
      181 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
      241 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
      301 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcaaca
      361 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      421 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      481 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagtcccttg
      541 cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      601 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      661 taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
      721 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
      781 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
      841 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
      901 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
      961 gcatggagcc ggccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg
     1021 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
     1081 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
     1141 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
     1201 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
     1261 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
     1321 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
     1381 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
     1441 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
     1501 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
     1561 catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag
     1621 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
     1681 aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagatgtt ttggc
//
LOCUS       DAZOYL010000454         1730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZOYL010000454 DAZOYL010000000
VERSION     DAZOYL010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.458"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            57..164
                     /locus_tag="ACHUOY_004106"
     CDS             57..164
                     /locus_tag="ACHUOY_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684701.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            370..1653
                     /gene="gltA"
                     /locus_tag="ACHUOY_004107"
     CDS             370..1653
                     /gene="gltA"
                     /locus_tag="ACHUOY_004107"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR0684702.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          454 a          418 c          404 g          454 t
ORIGIN      
        1 ctaatttgac aatcattcaa caaagttgtt acaaacattg ccaggaaaag catataatgc
       61 gtaaaagtta tgaagtcggt atttcaccta agattaactt atgtaacagt gtggaagtat
      121 tgaccaattc attcgggaca gttattagtg gtagacaagt ttaataattc ggattgctaa
      181 gtacttgatt cgccatttat tcgtcatcaa tggatccttt acctgcaagc gcccagagct
      241 ctgtacccag gttttcccct ctttcacaga gcggcgagcc aaataacaaa cgggtaaagc
      301 caggttgatg tgcgaaggca aatttaagtt ccggcagtct tacgcaataa ggcgctaagg
      361 agaccttaaa tggctgatac aaaagcaaaa ctcaccctca acggggacac agctgttgaa
      421 ctggatgtgc tgaaaggcac gctgggtcaa gatgttattg atatccgtac tctcggttca
      481 aaaggtgtgt tcacctttga cccaggcttc acttcaaccg catcctgcga atctaaaatt
      541 acttttattg atggtgatga aggtattttg ctgcaccgcg gtttcccgat cgatcagctg
      601 gcgaccgatt ctaactacct ggaagtttgt tacatcctgc tgaatggtga aaaaccgact
      661 caggaacagt atgacgaatt taaaactacg gtgacccgtc ataccatgat ccacgagcag
      721 attacccgtc tgttccatgc tttccgtcgc gactcgcatc caatggcagt catgtgtggt
      781 attaccggcg cgctggcggc gttctatcac gactcgctgg atgttaacaa tcctcgtcac
      841 cgtgaaattg ccgcgttccg cctgctgtcg aaaatgccga ccatggccgc gatgtgttac
      901 aagtattcca ttggtcagcc atttgtttat ccgcgcaacg atctctccta tgccggtaac
      961 ttcctgaata tgatgttctc cacgccgtgc gaaccgtatg aagttaatcc gattctggaa
     1021 cgtgctatgg accgtattct gatcctgcac gctgaccatg aacagaacgc ctctacctcc
     1081 accgtgcgta ccgctggctc ttcgggtgcg aacccgtttg cctgtatcgc agcaggtatt
     1141 gcttcactgt ggggacctgc gcacggtggt gctaacgaag cggcgctgaa aatgctggaa
     1201 gaaattagct ccgttaaaca cattccggaa tttgttcgtc gtgcgaaaga taaaaatgat
     1261 tctttccgcc tgatgggctt cggtcaccgc gtgtacaaaa attacgaccc gcgcgccacc
     1321 gtaatgcgtg aaacctgcca tgaagttctg aaagagctgg gcaccaaaga tgacctgctg
     1381 gaagtggcta tggagctgga aaacatcgcg ctgaacgacc cgtactttat cgagaagaaa
     1441 ctgtacccga acgtcgattt ctactctggt atcatcctga aagcgatggg tattccgtct
     1501 tccatgttca ccgtcatttt cgcaatggca cgtaccgttg gctggatcgc ccactggagc
     1561 gaaatgcaca gtgacggtat gaagattgcc cgtccgcgtc agctgtatac aggatatgaa
     1621 aaacgcgact ttaaaagcga tatcaagcgt taatggttga ttgctaagtt gtaaatattt
     1681 taacccgccg ttcaaatggc gggttaattt ttatatgccc aaacacaaaa
//
LOCUS       DAZOYL010000455         1715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZOYL010000455 DAZOYL010000000
VERSION     DAZOYL010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.459"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHUOY_004108"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHUOY_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR0684703.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..>1715)
                     /gene="pdeC"
                     /locus_tag="ACHUOY_004109"
     CDS             complement(448..>1715)
                     /gene="pdeC"
                     /locus_tag="ACHUOY_004109"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR0684704.1"
                     /translation="SLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRG
                     NYVAVINPLFWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQR
                     NGYLYATVYSTKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQN
                     YLSPKRKLQRALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLA
                     EKEGMIEQITDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQY
                     AVRPQQIKFEVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDI
                     LKIDKSFVETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQ
                     GWFFAKAMPPQVFMQWMEQLPARELTRGQ"
BASE COUNT          470 a          410 c          371 g          464 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatg
//
LOCUS       DAZOYL010000456         1700 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZOYL010000456 DAZOYL010000000
VERSION     DAZOYL010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.460"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            98..1351
                     /locus_tag="ACHUOY_004110"
     CDS             98..1351
                     /locus_tag="ACHUOY_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0684705.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1400..1519)
                     /locus_tag="ACHUOY_004111"
     CDS             complement(1400..1519)
                     /locus_tag="ACHUOY_004111"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR0684706.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
BASE COUNT          357 a          426 c          411 g          506 t
ORIGIN      
        1 atcttatttg agattattaa tatattagac agaacaattc gattttccta acctatgtat
       61 aagcctgatc cacaggcata tttagcaagg atttcaagtg agccgtacta caactgttga
      121 tggcgctccg gcaagcgaca ctgacaagca aagcatttct cagccaaatc aatttattaa
      181 acgcggtacg ccgcaattta tgcgcgtcac cctggcgctg ttctctgccg gactggcaac
      241 ctttgcactc ctctattgtg tgcagcctat ccttccggtg ctttcgcagg agtttggctt
      301 aacccccgcg aacagtagta tttcactgtc catttccacg gcgatgttgg ctattggttt
      361 gctgtttact ggccccctat ccgatgccat tggtcgcaaa ccagtgatgg tcacggcgct
      421 actgttggcc tccatttgta cgttactttc gacaatgatg accagctggc acggcatttt
      481 gattatgcgc gccttgattg ggctttcgtt aagtggcgtg gcagctgttg gcatgactta
      541 tcttagcgag gaaatccacc ccagtttcgt ggccttttcg atggggttgt atatcagcgg
      601 caactcaatt ggcggcatga gcggacgctt aattagtggt gtcttcacgg actttttcaa
      661 ctggcgaatt gctctggcgg caatcggttg tttcgcgcta gcctcggcgt tgatgttctg
      721 gaaaatcctt ccggaatcac gccattttcg cccgacttcg ctgcgcccta agacgttatt
      781 tatcaacttt cgtctgcact ggcgtgaccg gggattaccg ttattgttcg cagaaggctt
      841 tttgctgatg gggtcgttcg tcacgctgtt taattacatc ggctatcggt tgatgctttc
      901 cccctggcat gtcagtcagg ccgtggttgg cttattatcg ctggcttatt tgaccggtac
      961 atggagctca cccaaagccg gaaccatgac cacccgctac gggcgcggtc cggtgatgtt
     1021 gttttcgacg ggggttatgc tgtttggttt actgatgacc ttattcagct cgctgtggct
     1081 gatctttgcc ggaatgttac tcttctcagc aggattcttc gcagcccact cagttgccag
     1141 cagctggatt ggcccccgcg caaaacgcgc taaaggccag gcctcctcgc tgtatctgtt
     1201 cagttactat ctggggtcga gtattgccgg gacgctgggt ggtgttttct ggcataacta
     1261 tggctggaac ggcgtcggcg catttattgc tctgatgctg gtcattgctc tgctggtcgg
     1321 gacgcgtttg catcgtcgtc tgcacgcctg aaaaataagc ccggactgcg gtaactacac
     1381 gtccggactt attgcttgct caaaccaacg ttaatagcca tcctaaaata gacgaagcgc
     1441 cagccaattc ccgcagcgcg tctagcgtca tcaggattat aagtacccaa ataaacggat
     1501 tcattttgct gtgtgtcatt tattactgat gcgcagttat tctaccgctt tgtaagtagt
     1561 aaaatagtta acccgatcaa gactactatt attggtagct aaatttccct taagtcacaa
     1621 tacgttatta tcaatgctgt aatttattca gcgtttgtac atatcgttac acgctgaaac
     1681 caaccactca cggaagtctg
//
LOCUS       DAZOYL010000457         1696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZOYL010000457 DAZOYL010000000
VERSION     DAZOYL010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.461"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(846..1397)
                     /locus_tag="ACHUOY_004112"
     CDS             complement(846..1397)
                     /locus_tag="ACHUOY_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0684707.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
BASE COUNT          479 a          370 c          309 g          538 t
ORIGIN      
        1 tgatttaggt caaagacaaa agcattgaca aaatggtaag attgttcaca tgaccttact
       61 taacagactt ttaagagact ttgaaacagc ctgtttaaca ggtcactacc tctggtctta
      121 tcgttccctt ctttctaggt ggttatctgt taagagtttt tcagttttta ttcttaagat
      181 aattgttaag atgatactct cccttcgggt tatatcgtat ctcttctccc gttaaatatc
      241 ccttcacagt cagactaaat aacacctgtg tagttcttgg tagaatcttt caaggtaact
      301 tagctctgag tttatgaagt tgacctatga gatagactct accgaatctg ttagtcaatt
      361 gttgcttagg tgctgctaag gcttacttag tggaaagaga ggggatatgt gaccataggg
      421 gaacccctcc tgttaaaaga ctacttagaa gtctgaggtt ttagtaaaat ctgtgaatat
      481 ggcttctcag acgctctgta acgcattcta agcgatttta cccctttatt ttaaggtggt
      541 tagtacatcc ctataggtta cccctaaaaa tggggttaaa actcgctaca gagctttcta
      601 tgagattagc ttctaatcga atcaactatg aggtttggta ggattgtgca aaatctgtac
      661 agtaactttt cagaatctga aaatttttat aaaaaatgac tagccctctt tgaatctcat
      721 cttccccgaa tcttcaaaaa tatctgacag atgtctgtag ctgctaagta cccgtcacac
      781 cagctatgtg acccccttgg caaaaaagcc aaatatcagc tacatccatg taactattca
      841 gtaacttatt tagcaagtct gtaataagtt gctctagaga ttcctaactc ttcctgaatc
      901 tgcaacctcg tcttaccttg tgaaataagc gtctgtacag cctctgaaga agcttttaca
      961 ggcctcccca tatgtacccc ttcagcttta gcacgttcaa taccagcctt tctacgctct
     1021 gctatcaagc ttctttcaag ctgtgccaac gttgacataa tgcctaacat tgcttcaccc
     1081 atagcgcttg ataagtcaaa cccttctcta aggctgataa ctgtcacacc tttagcctgt
     1141 agcgctttaa ccgttgataa cacatcaatt gtgtcacgtc caagacggtc tacagctacc
     1201 acaactacgg tgtcaccttc tcgtgcataa gctaatagtc tgctgaaacc gtttcttaaa
     1261 gaagctttga cagaaccact gacagcttca tcagtgaacc atttagcaat gttgtaccct
     1321 gcctcttcaa tttgcatttg ctggttagtg atggattggt catctgtact caccctgcaa
     1381 tatccaagta tcgccataat gttcccctta gaagtctcaa aagttagtct cataagttta
     1441 gctgtctcat aatccaaaaa caaccttttg agactacttg agggtgcatt ctaccgttac
     1501 gttgaaagtg tctcaaaagg aagtcctttt aagactggtt atgaaggagc tatgaggttg
     1561 attaggggtt ggtacaagcc caactaaccg aatcaactat gaagatttta ggagggtaac
     1621 tgtaaccatc actttctaac tttcacgtag gaaattgtta agtggtttga tagttacacc
     1681 atgtgaccaa gcatct
//
LOCUS       DAZOYL010000458         1683 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZOYL010000458 DAZOYL010000000
VERSION     DAZOYL010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.462"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..789
                     /locus_tag="ACHUOY_004113"
                     /pseudo
     CDS             1..789
                     /locus_tag="ACHUOY_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            904..1245
                     /locus_tag="ACHUOY_004114"
                     /pseudo
     CDS             904..1245
                     /locus_tag="ACHUOY_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1203..1683
                     /locus_tag="ACHUOY_004115"
                     /pseudo
     CDS             1203..1683
                     /locus_tag="ACHUOY_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          424 a          406 c          496 g          357 t
ORIGIN      
        1 gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa atacgccacg
       61 atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg ctatcgtcgc
      121 ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt acgcagactt
      181 atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag ctcatattgc
      241 ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc ggagcaacct
      301 aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa agtctggctg
      361 tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag tacacgccct
      421 gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa tgctggcgaa
      481 cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg gctggaaaga
      541 gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc tgataatgct
      601 gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg taaatggtcg
      661 ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg gtataacgag
      721 cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca acaatgcggg
      781 ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat ttggattgtc
      841 cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa ctctcatgct
      901 gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag gcagacaccg
      961 gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac ggacttcgat
     1021 atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta cagtgtgccg
     1081 gaaagcctgt gtggccagct ggtatcaata cggatctcgc tggatgatga actgcggatc
     1141 tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata cggctggcag
     1201 accgtcaggc ccggatgttc atcagttaca gcgaagtcag cataaaaaat cccgacaaca
     1261 gcggccaggc gctcccgctc acgtatgtct gttgccggga gcaggcagag gacggtgcct
     1321 gctggcatct gctgacgtca ggaaaagcgg cgagtgccgc cgatgcacga cgtattgtca
     1381 gccattacga gcgacgctgg ctgacagagg aataccacaa ggcgtggaaa agtggtggta
     1441 catggaatcg ctgcgaatgc agacccggga taaccttgag cgcatggtgg ttatccaggc
     1501 gtttatagcg gtacgggtgc tgggcctgcg gcagggaggt gtcagtgaag aaacacaaaa
     1561 tgacagctgt gagaaaatac tgacgccgac agagtggaaa ctactgtggg ttaagctgga
     1621 aggaaagccg cttccggtgc aggcaccgac cctgaaatgg gcctggggcg atggcatgac
     1681 agc
//
LOCUS       DAZOYL010000459         1681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZOYL010000459 DAZOYL010000000
VERSION     DAZOYL010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.463"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            82..870
                     /gene="fhuF"
                     /locus_tag="ACHUOY_004116"
     CDS             82..870
                     /gene="fhuF"
                     /locus_tag="ACHUOY_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR0684708.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(908..1585)
                     /gene="bglJ"
                     /locus_tag="ACHUOY_004117"
     CDS             complement(908..1585)
                     /gene="bglJ"
                     /locus_tag="ACHUOY_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR0684709.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1543..>1681)
                     /locus_tag="ACHUOY_004118"
     CDS             complement(1543..>1681)
                     /locus_tag="ACHUOY_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFR0684710.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          435 a          440 c          387 g          419 t
ORIGIN      
        1 caagccagag aaatcccttg ctatcgggta aacctatcgc taggattagc aatcattatc
       61 atttagatta ctatcccgat tatggcctat cgttccgcac cgctctatga agatatcatc
      121 tggcgaacgc atctccagcc gcaggatgct gggcttgcac aagccgtacg tgcgacgatc
      181 gccaaacatc gtgaacattt gctcgagttt atccgcctgg atgaacccgc tccgcttaac
      241 gccatgacgc tggcgcaatg gtcatcaccg aatgcgctaa gttctctact cgcggtctat
      301 tccgatcata tctatcgcaa ccaaccaacg atgatccgcg agaacaaacc gctgatctca
      361 ctatgggcac aattgtatat cggcctgatg gtgccaccat taatgctggc gctactgacg
      421 caggaaaagg cattagatgt gtcgccggaa catttccatg ctgagtttca cgaaaccgga
      481 cgcgtcgcct gtttctgggt cgatgtgtgt gaagataaaa acgcaacacc acattcgccg
      541 cagcagcgaa tggaaacgtt aatcagccag gcgctggttc cggttgtgca ggcactagaa
      601 gcgaccggag agatcaacgg caaacttatc tggagtaata ccggttattt gatcaactgg
      661 tatctcactg agatgaagca actgctcggc gaggcgacgg ttgaatcgct gcgccatgcc
      721 ctcttttttg agaaaacgct cacgaacggt gaagataatc cactctggcg taccgtcgtg
      781 ctgcgcgatg gcctgctggt gcgccgaacc tgctgccagc gttatcgctt accggatgtg
      841 cagcaatgtg gcgattgtac gctgaaatga aagcactgcc ggggaagtaa acccggcatc
      901 atgcggatta atagggatgc aacacattac ttgcttcgca atgccgcata cataacagaa
      961 tatctgcggc ctccaacaac cctgcatcag aactgacgcc cagtttcgac atcacattaa
     1021 atttatgtgc acggatcgtt ttgatattgc gtttaagctg ctcggcaatt tgtgtcattg
     1081 agtagccacg cgacataaag cgcaatattt ctctctccgt cgggcttagc gtccggcttt
     1141 ggttaatgta ccactgattg ttcagtcggt cggtcgcctg acgtacacca tttaatgaca
     1201 aaaagagttc ctgatgaaaa atctccagcg ttgacgcttt acttaataca ccgtccagcg
     1261 gtgatggcga caatgaacca atcagccgag cttcaatatc atcatccgca ataactaaac
     1321 gccgggtacg cggaaactta atcgccagtt cagtcaggca agataatccc tctctgcgct
     1381 cacttctcat ggcagaaaaa gaaaaaatga ccgccgcaaa tgagatccgc gacatggcat
     1441 cctgaaatga ctcctgactg gtataggtat ggagcttata agggttaccc gcaaactttc
     1501 tgaataaact ctcaataccg atactgctca tgacgcattt ttctatgagt gcgacatttc
     1561 tcttcttaat tcggctgtgt tccattctgc ggcatctcca taaacagaag gcaagttaag
     1621 ttctcttgca ccctgcgcgc tatttatcca tgcatacatt tcagcgttgc tgtgaattgc
     1681 c
//
LOCUS       DAZOYL010000460         1676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZOYL010000460 DAZOYL010000000
VERSION     DAZOYL010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.464"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..889)
                     /locus_tag="ACHUOY_004119"
     CDS             complement(<1..889)
                     /locus_tag="ACHUOY_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0684711.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="ACHUOY_004120"
     CDS             complement(989..1345)
                     /locus_tag="ACHUOY_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR0684712.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="ACHUOY_004121"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUOY_004121"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          333 c          392 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactac
//
LOCUS       DAZOYL010000461         1670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZOYL010000461 DAZOYL010000000
VERSION     DAZOYL010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.465"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..483
                     /gene="cas6e"
                     /locus_tag="ACHUOY_004122"
     CDS             <1..483
                     /gene="cas6e"
                     /locus_tag="ACHUOY_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0684713.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
     gene            480..1403
                     /gene="cas1e"
                     /locus_tag="ACHUOY_004123"
                     /pseudo
     CDS             480..1403
                     /gene="cas1e"
                     /locus_tag="ACHUOY_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            1400..>1670
                     /gene="cas2e"
                     /locus_tag="ACHUOY_004124"
     CDS             1400..>1670
                     /gene="cas2e"
                     /locus_tag="ACHUOY_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR0684714.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVS"
BASE COUNT          394 a          388 c          490 g          398 t
ORIGIN      
        1 cgcttttttg tcctgtcgca ggaacgcccg gcggaaagtg atattttcac catcgaatgc
       61 cgaccatttg cgccagagtt gcgtaccgga caatcccttt gtttcaacct gcgggcgaat
      121 ccaacaattt gtaaagcggg caaacgccac gacttgctga tggaggcgaa acggcaggtg
      181 agggggcagg tggaaggaag tgatgtctgg ttgcatcaac aacaggctgc gctggactgg
      241 ctggcagcgc agggggagag aagcggtttt acactgctgg atacttcggt tgatgcctac
      301 cgacaacagc aactgcggcg ggaaaactcc cggcaactga tccagttcag tagcgtggat
      361 tatacgggga tgcttacggt caccgctcct gggttatttc tgcaacgtct cagtcagggg
      421 tatggcaaaa gccgggcgtt cggttgtggt ctgatgctga tcaaacccgg agcagaggcg
      481 tgacgtttgt accgctgagc ccgatcccat taaaagatcg cacttccatg atcttcctcc
      541 agtacggtca aatcgacgta ctggacggcg ctttcgtgct gatcgacaaa accgggatcc
      601 gcacgcatat tccagtggga tcggtcgcct gcattatgct cgaaccggga acaagagttt
      661 cccacgcggc agtccatctg gccgccacgg tgggaacact gctggtctgg gtcggtgaag
      721 cgggcgttcg cgtttactcc tccggacaac ccggaggggc gcgggcagat aaattactct
      781 actaggcaaa gctggcttta acggaagatc tacggttgaa ggtggtgcgc aaaatgtatg
      841 aattacgttt tcgtgaacca ccgccagctc gccgttcagt ggagcagtta cgtggaattg
      901 agggatctcg cgttcgccag acgtatgcat tactggcgaa acaatatggt gtgaaatgga
      961 atggtcgcaa atacgatcct aaagactggg aaaaaggcga tgttgtgaat cgctgcatca
     1021 gtgctgctac atcatgtctg tacggtatat ctgaagcggc agtattagcc gcgggatatg
     1081 cgcccgctat tgggtttatc catagtggca aaccgctttc atttgtttat gacatagccg
     1141 atatcattaa atttgattcg gttgtgccaa aggcatttga aatagcagcg aggcaacccg
     1201 cagaacctga taaagaagtc agattagcct gtcgcgatat tttccgtagc actaagttaa
     1261 cgggcaaatt aataccgtta attgaggaag tccttgctgc aggtgaaatt gaaccaccac
     1321 aacccgcgcc ggatatgtta ccgcctgcca tccctgaacc tgaaacgctg ggtgatagtg
     1381 gtcaccgggg gcgcggcgga tgagcatggt cgtggttgtt acagaaaatg tccctccgcg
     1441 cttacgtgga cggctcgcaa tctggctact ggaagtgcgt gccggtgtgt atgttggtga
     1501 tacatcaaaa cgtattcggg agatgatctg gcagcaaatt acccaactgg ctggttgcgg
     1561 aaatgtggtg atggcctggg cgaccaatat cgagtcgggt tttgaatttc agacctgggg
     1621 agaaaacaga cgtattccgg tggatttgga tgggttacgt ttggtttctt
//
LOCUS       DAZOYL010000462         1668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZOYL010000462 DAZOYL010000000
VERSION     DAZOYL010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.466"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..959)
                     /locus_tag="ACHUOY_004125"
     CDS             complement(<1..959)
                     /locus_tag="ACHUOY_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0684715.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            complement(1560..>1668)
                     /locus_tag="ACHUOY_004126"
     CDS             complement(1560..>1668)
                     /locus_tag="ACHUOY_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HFR0684716.1"
                     /translation="DGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          439 a          279 c          410 g          540 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatctgcgg gccgtggtag tccggcgaag aggttaatct ttgcagggat
      181 tgcagagcat gggatgacaa tgggttatca ctaatatcta ttgaacattc attcctcaga
      241 ttaagaatgc tttccgggat atgacttatc tgatttatgt caaaataacc gttccttaaa
      301 ttttccggca ggtatggaat atcggttaac atattgtcga ttgcccatat ggagtgaatg
      361 ttgtgaggta gaggggggag ttcactaaga agattcccta ttgcgctaat ctcttgtaaa
      421 gaaaatggta aaggtggtaa aactgccagc ccattacatg atacatctaa tgtttccagt
      481 agttctggta aaacaggaag agaacataat tgattatttg agacatgaag ctccttcaaa
      541 gatatgggga gtgtgggtag tgtgattagt tgattgtggg acgcattcaa taatttaagt
      601 ccttgaggca aagcaggcag ttcaataagt ctgttatagc tgacattaag ctgtgtaagg
      661 gacgcaggca atggggagat taagcttaaa ttatttttac ttatattaat tgatttaatt
      721 tccgggggga tttcaggtaa tgttgtcagg cctaattcag acaagtccag gttcgtctct
      781 tggttttgta gacatgatac tagtcgctga aaagcgatgt ctcgttgttc ttcttgtatg
      841 cggttatttt tccattcagt ccaatgggtt agataatttt catatgcacg gctagtgtcg
      901 attgaataag tggagaacga atttgaaatt aatctgtgat tgttatttgt cgggagcata
      961 aatatcaggg cgtattcttt atgttgagag gtgttggatt cttttttatc tgctttgcaa
     1021 tgttattggt tcccttcagc agtagacagg aacttctgga ggaggcgaat atatagtttg
     1081 tgtaagcata ttaactccat atgttatata ttgaagaact tctgctttac actatcctaa
     1141 actggatcgg tttaatgtaa aaaaacgata ctttttgggg gagggggcaa tttttcctta
     1201 aggtatgaaa aaaggagctg aagctatata atagctatta atgccactgg tatcagtgaa
     1261 tcatgtatat aaaaaactgt gttaaggagg ctgtccatta gcggtttttt attcttctgg
     1321 aaccgcggca tctttgcgta tctttttgtc ttctcaccgt ctggtcggtg ttttgctgag
     1381 actgctaatt tcctgtcttt gctagtgctg tactggtact gcccaatcat gattggcggg
     1441 ggcgggcgga gttgagaccg cagacacgtc gtatgcaaga acgtgctgcg gctggctggt
     1501 gaacttccga tagtgcgagt attgaatgat ttccagccgt tactgatttt acgtgctaat
     1561 tagtgaacaa accactcgtc agcagactcc caggtatctt tcagagtttc ctgaacaaaa
     1621 gttttagctg aatctttatc ggcggtgcgc gtaacagaaa ggccatcg
//
LOCUS       DAZOYL010000463         1667 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZOYL010000463 DAZOYL010000000
VERSION     DAZOYL010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.467"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUOY_004127"
     CDS             complement(<1..105)
                     /locus_tag="ACHUOY_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684717.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGK"
     gene            complement(183..604)
                     /locus_tag="ACHUOY_004128"
                     /pseudo
     CDS             complement(183..604)
                     /locus_tag="ACHUOY_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            complement(588..815)
                     /locus_tag="ACHUOY_004129"
     CDS             complement(588..815)
                     /locus_tag="ACHUOY_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR0684718.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYAPKVYDEYRKAKRPWKVIHENQA"
     gene            892..1299
                     /locus_tag="ACHUOY_004130"
     CDS             892..1299
                     /locus_tag="ACHUOY_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0684719.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1503..1658
                     /locus_tag="ACHUOY_004131"
     CDS             1503..1658
                     /locus_tag="ACHUOY_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0684720.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          470 a          436 c          344 g          417 t
ORIGIN      
        1 cttccctaat gcggcatctg gatcaatatt cagaatctca gcaagcctga atatttccgg
       61 cttatccggc gtaataacct cgagctttat ccagtttgaa gccatttgtt ttcaccttgt
      121 aacgctcgca gcgttacatt taactgatac cgaacaaaac aatccggcac gattaatttc
      181 aatcaatgca ctacgacaga atcgccgggc gacccaccac cgctgaaatg tgctttccgg
      241 taaacggcct ggacagcatc atcatgcgca tcaattgccg tactcaacgc ttcctgcgcc
      301 gccagtaatg cacggcgttc cagggtatcg aagatacaga gtcggtgacg cagctcacgc
      361 ggaagaattg ccagaaccgc agggatcagt ttctgaattt tttccctttg cgctttcgtt
      421 tcacctttca accaacggtg atagatattc tgctgattgt tccagtcctt gcctggtacc
      481 agagacaatt cgccgccccc ctgccgcaga tattcttcag taattgcgtt agcgacccac
      541 gcctgccctt tttcggctgc cagggcaaca gcactgattc gatgtgctca tgcctgattt
      601 tcatgaatca ccttccaagg tcgttttgcc ttacgatatt cgtcataaac tttgggggcg
      661 tactgaagtt ccccgccaga tgcctcttgc aggcgcatcg cgcgaccttc aggaaccagt
      721 tccccccatg cagcaatgct tgccagccta actcctgcgg cattggcaag ctttgttttg
      781 ctgccaaaaa acgctatagc atcaattttc aacatatcga gctccttaga ttttcctaag
      841 gaaactagat cgtagagaaa cctaagtcaa gaaaaattag aattccctaa tatgaaaaac
      901 gaaaccttcg gtgctcgcct cttacatagg cgtaaaaaat taaaactgtc tcaggccgca
      961 ttaggtaagc tggtcaaagt ggctcacgta acaatttctc aatgggaaag agatgaaaca
     1021 cagccggcgg ggaaaagatt attcgcactg agccaagcgc ttcagtgctc accgacttgg
     1081 cttctttttg gggatgaaga taaacaacca ggcgaaccga tcccggataa tcagccagcc
     1141 attctgacag aagatcaaaa agagttactt caactgttcg acgcactgcc tgagtcagag
     1201 caaaaggccc tgttgtcaga gatgcgtgct cgagttgaaa atttcaacaa actttttgaa
     1261 gaactactca aagctcgcaa aagaagcgca aacaaataat cccctttttt ctccacaccc
     1321 tgtaataaaa agcacaaact ttcaaacact tgtgtttttt acatcaaaaa aacttaggtt
     1381 tttctacaca aaaatcttga ccgcccacct taggttattc taaatttcat tcatcaagac
     1441 accgcacggt gttctcagca aacagttccg ctaccccggc gttaagggga aatggggtca
     1501 acatgaatac tatcgatctt ggcaacaacg aatctctggt gtacggcgtg ttccccaacc
     1561 aggacggcac attcaccgcc atgacgtata ccaaaagcaa aacgtttaaa accgaagctg
     1621 gcgcacgtcg ttggttggga agacattcag gtgagtaaaa tgaacga
//
LOCUS       DAZOYL010000464         1639 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZOYL010000464 DAZOYL010000000
VERSION     DAZOYL010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.468"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..291)
                     /gene="gadE"
                     /locus_tag="ACHUOY_004132"
     CDS             complement(<1..291)
                     /gene="gadE"
                     /locus_tag="ACHUOY_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR0684721.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKN"
     gene            complement(1090..1638)
                     /gene="hdeD"
                     /locus_tag="ACHUOY_004133"
     CDS             complement(1090..1638)
                     /gene="hdeD"
                     /locus_tag="ACHUOY_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR0684722.1"
                     /translation="MLKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILSTV
                     VGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIAAF
                     IAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVSTLV
                     GIELIFSAASLFSFASLFVKQQ"
BASE COUNT          534 a          345 c          299 g          461 t
ORIGIN      
        1 atttttgata ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact
       61 gatgtgataa ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat
      121 cgcttcttca tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt
      181 gcctactttt ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa
      241 ctgccaaaag ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc
      301 cttagccgtt atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc
      361 gttgttcaat atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt
      421 aacactaatc aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag
      481 attcctggtt gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg
      541 cccaagaata agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa
      601 atgtaatccg atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc
      661 cagctttctt ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt
      721 tttatatagc gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa
      781 aatgaacccg gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt
      841 cactttccca cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata
      901 atgtttgaaa tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg
      961 aaataatcac tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg
     1021 tagcggagag agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa
     1081 ccgggataat tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct
     1141 aaatatcagt tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc
     1201 accaaggaat atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct
     1261 gcctttcatt gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa
     1321 caaaccggca ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa
     1381 atagccgatc aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt
     1441 atgactgcgg ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc
     1501 acccactact gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc
     1561 gacgataaac agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc
     1621 cagatcaaac ttcaaaatt
//
LOCUS       DAZOYL010000465         1630 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZOYL010000465 DAZOYL010000000
VERSION     DAZOYL010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.469"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..508)
                     /locus_tag="ACHUOY_004134"
     CDS             complement(<1..508)
                     /locus_tag="ACHUOY_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFR0684723.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVV"
     gene            984..1091
                     /locus_tag="ACHUOY_004135"
     CDS             984..1091
                     /locus_tag="ACHUOY_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0684724.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            1467..1574
                     /locus_tag="ACHUOY_004136"
     CDS             1467..1574
                     /locus_tag="ACHUOY_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0684725.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          465 a          373 c          439 g          353 t
ORIGIN      
        1 tgaccaccac cattggcccg gaaatcttaa acagtaatcg ttcaccacca gacgcaatcg
       61 ctaccagtac ggcgaaaata gcgactttat aaagtagaga ttgtgaaaga tcggcatcgg
      121 ttaaaccgaa ggttttcagg tacgaggcgc tgtcgaaaac cacggagaga gagtagataa
      181 aaatcccgtg gataatcatc aaaaagtaga taaccccgag gaaaattccc cagttcttcc
      241 ccaggtaatg actgataata tcggtgtagt cattacagga atcgctttca gaaagggttt
      301 ttaaataaat gtcctgcact acccaggtgg caggataagc aatgatcgct gcggtaataa
      361 atacccaaat tcccttcaag ccaatttgta ctggcatcag cacggttccg gcaccaatcg
      421 ccatgcctat gcataataaa acccagccga agtcgtagcg tgtaaacggc aatttctgat
      481 tgtgtttcga tagtgtgttg tgctgcataa agtcctgtcc agaaagaagt cggcctcgca
      541 aaaatgaata aacccagttt ctcttgcgca acaggtggag aagtcgaata cgcagtatgt
      601 aaaaaatgag gcaacagatt cactggggag gtcgcgagga ggcgcaattc tacaaaattg
      661 cgcatttcac taccagtctg gagtcagccc ggatatgaac tgcaactatt gcaaataaag
      721 aaattaagct ggaaggcatc gcgcaatgag gaagattgcc gggtgctgac agtaaaaagc
      781 aatcgtaaaa tcagcggcaa gccgaaaggg ttccggtgag gcgcaatgtt gcgggggctt
      841 tatccctggt ggcattggtt gctggagaga gaaaaccccc gcacgttgca ggtatgtacc
      901 tgacaacata acgggggcta atcttgaacc tagaccactc aagaatagcc gcgaaacgtt
      961 gtcattacaa cacaggcggc tatatgacgc tcgcagagct gggcatggcc ttctggcatg
     1021 atttagcggc tccggtcatt gctggcattc ttgccagtat gatcgtgaac tggctgaaca
     1081 agcggaagta gcgtgtcatg tgggcgtcag gctgccgtaa tggcaatttg cgcccggacc
     1141 aggccgcagg agtgaaactc tgcggccttt ttcgtaagaa aaatgcaacg gttgcaagcg
     1201 gataatattc tgcggaatgc gcagcacaga gtgaagatga gacggattcc ggaacagaaa
     1261 aacagtcgta gaatcagcgt caagccggaa ggattccggt gaggcgcaat gttgcggggg
     1321 cttaatccct ggtggcattg gttgctggag agagaaaacc cccgcacgtt gcaggtatgc
     1381 acctgacaac accacggggg ccattcttgc atccaaacaa tacaagaata gccgcgaaac
     1441 gttgtcatta caatacaggc ggctatatga cgctcgcaga actgggcatg gccttctggc
     1501 atgatttagc ggctccggtc attgctggta ttcttgccag tatgatcgtg aactggctga
     1561 acaagcggaa gtaacgtgcc atgcgggcgt caggctgccg taatggcaat ttgcgcccgg
     1621 atcaggccgc
//
LOCUS       DAZOYL010000466         1626 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZOYL010000466 DAZOYL010000000
VERSION     DAZOYL010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.470"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..376)
                     /gene="nfsA"
                     /locus_tag="ACHUOY_004137"
     CDS             complement(<1..376)
                     /gene="nfsA"
                     /locus_tag="ACHUOY_004137"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0684726.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLG"
     gene            complement(360..647)
                     /locus_tag="ACHUOY_004138"
     CDS             complement(360..647)
                     /locus_tag="ACHUOY_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR0684727.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            807..1064
                     /gene="grxA"
                     /locus_tag="ACHUOY_004139"
     CDS             807..1064
                     /gene="grxA"
                     /locus_tag="ACHUOY_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR0684728.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(1094..1471)
                     /locus_tag="ACHUOY_004140"
     CDS             complement(1094..1471)
                     /locus_tag="ACHUOY_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR0684729.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
BASE COUNT          460 a          401 c          375 g          390 t
ORIGIN      
        1 cgcccaatcc cagcgattcc gctgcggtta atgcattctg cgccatcatt gccgtatcaa
       61 cgacaccgag caacagttgt tccgccaggc cgagctgagc atccggacag atctgtaaat
      121 ggcggttaaa gtcggcacag aacacccaga actccgccgc ttgcgctacg tgtttttgcc
      181 cgccggtcag cgtcaccagt tcttcacgta atgctttgtc ggtaatgcga ataatgctac
      241 tacactgcaa aaaactggaa ctggacgtcg cacgggcgct gttaataatc gcctcacact
      301 gcgcttcgga aatgggttca tcagtgaaat ggcgaatgga gcgatggcca caaataagtt
      361 caatggttgg cgtcattatc tttttctctt tctgaacgtg aatattgcgg tggacggttc
      421 atcagctgtg gggcaagacg ttttgccacc tgaagaataa ccaccaccgc agcgggaagc
      481 atgagcaaaa caccgagaaa aatcatcaga atctgcactt ctggccgaga aaatggctca
      541 ggcagcgaca gggagtcgct taccgacagc agcgccaccg ccagtagcat cattccgata
      601 aattccagta tcaacacgcc tttaggcaat ttaccgatcg cgcgcatacg cttccctctg
      661 caaagtgagc cttcagtcta aaacttttca ctgtattgtg tttaacagtt atagctttta
      721 gcaattaatg caacaggtta aacctacttt cagcgaatac attttagcgt gatcattaca
      781 ggcataaatc tatgaggaga gaaataatgc aaaccgttat ttttggtcgt tcgggttgcc
      841 cttactgtgt gcgtgcaaaa gatctggctg agaaattgag caatgaacgc gatgattttc
      901 agtatcagta tgtagatatt cgtgcggaag ggatcactaa agaagatcta caacaaaagg
      961 caggtaaacc cgtagaaacc gtgccgcaga tttttgtcga tcagcaacat atcggcggct
     1021 ataccgattt tgctgcatgg gtgaaagaaa atctggacgc ctgatcgtct gacaagccct
     1081 cgcgttgagg gctttactga ttttttctgt gctgtggttt aaacaaacta ctgataaata
     1141 agaaacacag tgcccccagc gcacaccaga acaccgcgct tagtaaccat gccagctctt
     1201 gccagaatga gcgcgtcggt gaaaaaaaca gccgcataat gagcattgaa cagggtgccg
     1261 ccagcattgc gccaaataga ggtttcagga cttctctacg ctgtgaaaag aaactggcaa
     1321 ctgctccagg aagaatgaaa aacagcaagc cgatttcagg atgcccggca gcccgaaaag
     1381 cgcctttcat gtgcgtcgcc agaaaaaggc acaccacaat gaagaggaca aaacagcaga
     1441 ttgcccccgc ccaacgttgt ttatgtttca ctcgttcctc ctgacactgc gtctatcgaa
     1501 cacatttttc gccagtgtgg cgttcagtaa gataaagccg cttcgcattc catgctaata
     1561 taggccaacg caattcatat agccgttgat acctaatgtg attacactag taaaatatat
     1621 tgttac
//
LOCUS       DAZOYL010000467         1618 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZOYL010000467 DAZOYL010000000
VERSION     DAZOYL010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.471"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..202)
                     /locus_tag="ACHUOY_004141"
     CDS             complement(<1..202)
                     /locus_tag="ACHUOY_004141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0684730.1"
                     /translation="MAEPMAQRPICRQNSHRLHDRLGEPQYTDSLHSQGSASSMWMRH
                     VGGHERFRTGDGQLNTQANRYVL"
     gene            complement(162..1618)
                     /locus_tag="ACHUOY_004142"
                     /pseudo
     CDS             complement(162..1618)
                     /locus_tag="ACHUOY_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
BASE COUNT          365 a          464 c          361 g          428 t
ORIGIN      
        1 gcaatacata gcggttagcc tgagtattta gctggccgtc accggtcctg aaacgttcgt
       61 gccccccgac atgacgcatc cacatactgc ttgccgatcc ctgagaatgc agtgaatctg
      121 tatactgcgg ctcacccaga cggtcgtgta agcgatggct attttgccga catatcggcc
      181 gttgcgccat aggctccgcc atgttcggct aacgtcagct tttgcccacc atggatgttc
      241 agcgctttca gcccatagtt aacgtacaaa ccatcaccta gtgcggtcgt gaggcggaat
      301 ccataatcac cctcggcgac caccgttccg ttttgcgcaa tgtgtaacgt tttgtccgct
      361 tccacctcgt cgccctgtaa atcacgtaac gttaatgagc cacccgaccc aataaccgtt
      421 tgcgccttca ccagttgaac gccgacatgg ctatcatcgt gttccagcaa attgagcgtc
      481 gtcagagggt tattcgctat gggatcattc cacggtttag gcacgtcgat aagcacgtcg
      541 cccgtcccgt ttacctgata gttacggcct ttccaggtgt agtcacccgc gccgacaacc
      601 agcgtatcga cgctgatata tccctctgca agcgtcgcag caggaatatc cgtatcgaaa
      661 atgagcgtac cgccattcat ggccagtcca ccaatggatt gctctcccac gtttaccgat
      721 gtggtatttt cactgtcaga ctgcaacatc gcgtgagtaa gcgcagcggt gttgtcgcgt
      781 tccagagtga aggttctgtc tttcagttgg gcaacaccgg cgaattcagt ccctgttgca
      841 tgggtaaagc caaacatctt gtcgtaggat gacagctgca ctcgcatcaa gccatcgcct
      901 ttgagcgcat tggtcagcgt gtaatctcct gcactgttcg ttgatgcgag aatgtcgagc
      961 gtaccggggc ttgcaatggt gacttcactc gaacccatgc ccgttacccc atctgccgta
     1021 tgagacgcaa tggtcaggag gccgtcattg accagcgttt taccgctata agtattccct
     1081 gcattgaaca ccgtatggcc tgcatcatgg atcacttttc cgtctttatc gtcacctgta
     1141 atcagcatat cgacctggta gccggcatca ctgttattag tatgattaaa gacaaaaaca
     1201 ccttcgccag aaccaaactc tactttcgtc gcgttggtta tatacccggc atctgccgcc
     1261 gcctcaccgt gagctgcacc aatgtttagc gttcctacag caggttcaaa aacacctaag
     1321 taaacttctc caccttcgac attcagcgtg ccgctattcg tcagggttaa cgtacccgta
     1381 ccagatgtcc ctacgtacat attgaatgtt tcaagaagag agttctgccc atccaccctc
     1441 acgtcgccct tacttttgtc attgagagct atctgtgtat cacgcgcttt aactatacct
     1501 ccggtggtaa tattcagttc acccgtgcct aatacaccga cctgtagcaa ttgtgcgtta
     1561 gtagatgatg tctttaaatt ccagagactg tccgttgaaa tattaactat ccccttac
//
LOCUS       DAZOYL010000468         1616 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZOYL010000468 DAZOYL010000000
VERSION     DAZOYL010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.472"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            184..1413
                     /locus_tag="ACHUOY_004143"
     CDS             184..1413
                     /locus_tag="ACHUOY_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0684731.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT          409 a          349 c          332 g          526 t
ORIGIN      
        1 aagtgatttt tatcacaaag gaaatatgcc tgagcagtag tcagagtcat aactggcacg
       61 taaggtttgc aaccactaac ccaccaatag atgggtagat aaggcgttaa tctcccatac
      121 ttaacctggt ttatggtaaa ttgccctcca ttttgtttaa tttgtagatg atacgttcag
      181 ataatgtcta atatttggtc taaagaagaa actctgtgga gtttcgcgct ctacggcaca
      241 gccgttggtg caggcacgct cttccttcct attcagttag gttcggcagg ggctgtggtc
      301 ctgtttatta ctgctctggt cgcctggcct ttaacatatt ggccacataa agccttatgc
      361 cagttcatcc tctcatcaaa aacatcagca tgtgaaggga taacgggcgc ggtaacacac
      421 tactatggca agaagattgg taatctgatt accacgctgt acttcatcgc cttttttgtc
      481 gtcgtgttga tatatgcagt ggcaattacc aactcactta cggaacagct ggcaaagcat
      541 atggttattg atcttcgcat ccgtatgttg gtgagtctgg gtgttgtatt aattctgaat
      601 ctcatttttc tgatgggacg tcatgccact attcgggtaa tgggattttt ggtattccca
      661 ttgattgcct atttcttatt tctttccatt taccttgtcg gtagttggca acctgatcta
      721 ttaacgaccc aggtagagtt caatcagaat acccttcacc agatatggat atcgattccc
      781 gtgatggttt tcgcctttag ccatacgccc attatttcta cgtttgccat agacagacgt
      841 gaaaaatatg gcgaacacgc tatggataaa tgcaaaaaaa ttatgaaagt cgcttatctc
      901 atcatctgca taagtgtact gttctttgtc tttagctgcc tgctttctat tccaccttcg
      961 tatattgaag cggctaaaga agaaggggtc accattttat cggcgctttc tatgctgccg
     1021 aacgccccag catggttgtc aatttccggg attattgtcg cagtagttgc gatgtcgaaa
     1081 tcattcctgg gtacgtactt tggcgttatt gaaggtgcca cggaggtcgt caaaacaaca
     1141 ctacagcagg ttggtgtaaa gaaaagtcgt gcatttaacc gcgcactatc aattatgttg
     1201 gtatcgctga ttaccttcat tgtttgttgc attaacccga acgcgatttc gatgatttat
     1261 gcgatcagcg gcccgctcat tgccatgata cttttcatca tgcctacgtt gtcaacgtat
     1321 ctgatcccgg cgcttaaacc ctggcgctcc atcggaaatc tgattacgct gatcgtgggt
     1381 atcctgtgcg tatcggtaat gttctttagc taatatccgt tgtggatggt tcaaaattgc
     1441 tacaattttg aaccattcca tttttcataa tattcatttg ttattattcc gccctatcac
     1501 ttctctcgtc atatcctaga attatcagtt agttaaacac acaaagagat ctgaacatct
     1561 taaataagac tatttaagat gcataactta gattcgcaag atatcttctg gtcatt
//
LOCUS       DAZOYL010000469         1575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZOYL010000469 DAZOYL010000000
VERSION     DAZOYL010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.473"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(497..>1575)
                     /gene="ychF"
                     /locus_tag="ACHUOY_004144"
     CDS             complement(497..>1575)
                     /gene="ychF"
                     /locus_tag="ACHUOY_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR0684732.1"
                     /translation="GIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDP
                     RLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCF
                     ENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKAELAVL
                     EKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPYLDQVR
                     EIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKLLNLQT
                     YFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQGAKEA
                     GKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT          378 a          372 c          366 g          459 t
ORIGIN      
        1 tgtatctgca taacaaaaac aatatgcgcc atttttgcat tactaataag aaaaagcaaa
       61 aaacatcgat attcattttt ggtcaatggg ttttctggcc tatcgttgtt attgtgctac
      121 tggttatcat tttcatcttg ccaggatcgc acgtaatcac gttattcggg gactttatct
      181 gcgggatatt acccttcagg taagtccttt tctcgccagc atattggagc gtgatcaata
      241 ttgatcagct gtgaaggggg tgatgtgtag ttgaacaaaa acttacgtta tatcactact
      301 aatgagatat ccgtcatctg acactgatgt ctttaattat tctcgtggtt cactgtaggc
      361 aataagcaca aaagtgtagg atgttacaag aatgattagg actcggtgaa atgaaaaatc
      421 cacgcaattg cgtggatttt atatactttt gcgctcttca tgagatttag cgaaacctca
      481 tgagacaata aattaattag acgttgaaaa ggaagttcat cacatcgcca tctttaacga
      541 tgtaatcttt accttctgca cgcattttgc ctgcttcttt cgcgccttgt tcacctttgt
      601 aagtgatgaa atcttcaaac gagatggttt gtgcacggat aaagcctttt tcaaaatcag
      661 tatggatttt gcccgctgcc tgcggcgcgg ttgctccaac cggaatggtc catgcacgca
      721 cttctttcac cccagcggtg aagtaagttt gcaggttcag cagcttataa ccgacacgga
      781 tcacacggtt caggcccggt tcttccagcc caagctcctg cataaactcg tcacgttctt
      841 cgtcgtccag ttcggcaatg tctgcttcaa cagcagcaca aaccggaacc acaacagaac
      901 cttctttcgc cgcgatttca cgcacctggt caagatatgg gttgttttca aaaccgtctt
      961 cgttgacgtt ggcgatgtac attgttggtt tcagcgtcag gaagctcagg taacgaatag
     1021 ccgctttctc ttcagcgctt aaatccagcg cgcgcagcat acctgcgttt tccagctggg
     1081 gcaggcattt ttctaggacc gccagctcag ctttcgcgtc tttatcgcca cctttggctt
     1141 tcttctgtac gcgatgaatc gcacgttcgc aggtgtcgag gtctgccagc gccagttcgg
     1201 tgttgataac ttcaatatcg tcagccgggt taactttgcc ggaaacgtga atgatgttgt
     1261 cattttcaaa gcagcgaaca acgtgaccga tcgcttcggt ttcacggatg ttggtcagga
     1321 actggttacc cagaccttcg cctttcgatg cgccttttac cagaccggcg atatcgacaa
     1381 attccatggt cgtgggaagc gtacgttgcg gttttacgat ttcagccagt tgatccaggc
     1441 gagggtcagg cattggtacg acgcctgtgt tcggctcaat ggtgcagaat ggaaagttgg
     1501 ccgcttcaat accggctttg gtcagcgcgt tgaacagggt agatttcccg acgttgggca
     1561 aaccgacgat accgc
//
LOCUS       DAZOYL010000470         1560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZOYL010000470 DAZOYL010000000
VERSION     DAZOYL010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.474"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            276..404
                     /locus_tag="ACHUOY_004145"
                     /pseudo
     CDS             276..404
                     /locus_tag="ACHUOY_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            594..998
                     /locus_tag="ACHUOY_004146"
     CDS             594..998
                     /locus_tag="ACHUOY_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684733.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1061..1560
                     /locus_tag="ACHUOY_004147"
                     /pseudo
     CDS             1061..1560
                     /locus_tag="ACHUOY_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
BASE COUNT          444 a          364 c          407 g          345 t
ORIGIN      
        1 tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt aataatcctc tcccacaaag
       61 tgaattaaat gaatatataa tgcgttcgaa gtatatgtat tgagctgtca tactgagaaa
      121 caaaccttag ggcagcacaa ttaaagccag aaacaactat gcaagaaagt tactaaataa
      181 gtgctcggaa cagtaaaaca ctcagggggg actcagtccg gattatgtaa tcagataaat
      241 tgccaaacat cacaaatcac cagccggact gagtgatgcc gagcattgca ccaatacccc
      301 gtgatgaacg atgcctgatg cagaaagctc cctataaaac aaagaacaaa aactacgacc
      361 ggcgattcac cgtcatactg atgctgcatc gtggtgacac tgtctgtcac tttcatgttg
      421 actgaacaac tctgctgcga agaaggtgta ccgccggcgg gatttgattg tccggcgtct
      481 agcttccact tccctgatgg aactgaaagt tgttatggcc ggtcagcttg ccctgtccgc
      541 aggacatgag gtgctgtgac ggacagcacc agtaacggaa agtgacaatg aaaatgaata
      601 ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt acccgctgct gcaaaagaga
      661 ctttactggc tatgccacgt aattcaacag aacattgcta tgcggagatt aatgtgcacg
      721 gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc cgggggaaag agctgggtcg
      781 aatgtaacag tgactactat tactctgata aaccaccggg agtacagata ctcggaaccc
      841 gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc gacactgcat gtggctggaa
      901 gaggggttgt gtgtattaaa aatatatgca gtccacgtgg gatgataatt cacaggatac
      961 ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc ttacccagca atagtggaca
     1021 cgcggctaag tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa cagtatcaac
     1081 cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc agtgaagccc tgaagcttgc
     1141 tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc ctgtatgaat ctcagctcta
     1201 tgcgtggcgc agcaaactgc agcaacagat gacctcgtct gagcgtgaaa gtgaactggc
     1261 cgctgaaaat gcccgcctga agcggcaact ggcggagcag gcagaggaac tggctatcct
     1321 ccaaaaggcc gcgacatact ttgcgaagcg cctgaaatga agtatgtctt tattgaaaaa
     1381 catcaggctg agttcagcat caaggcaatg tgccgcgtgc tccgggtggc ccgcagcggc
     1441 tggtatacgt ggtgtcagcg gcggacaggg ataagccctc gtcagcagtt ccgtcagcac
     1501 tgcgacagcg ttgtcctcgc ggcggccttt acccggtcaa aacagcgtta cggtgcacca
//
LOCUS       DAZOYL010000471         1536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZOYL010000471 DAZOYL010000000
VERSION     DAZOYL010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.475"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..652
                     /locus_tag="ACHUOY_004148"
     CDS             <1..652
                     /locus_tag="ACHUOY_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684734.1"
                     /translation="QKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQ
                     IQTVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSS
                     QVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLT
                     KDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            728..>1536
                     /locus_tag="ACHUOY_004149"
     CDS             728..>1536
                     /locus_tag="ACHUOY_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0684735.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFD
                     IRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALW
                     QVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTFHRAAEGD
                     VAGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVSSSQV"
BASE COUNT          343 a          392 c          500 g          301 t
ORIGIN      
        1 gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga taaacaacca
       61 tgccgaaaaa ataggtaaca accaggcgat caccgttacc aataaccaga tccagaacat
      121 tggcgttaat cagatacaga cggttggtgt caacctggtg gaaacggtgg gcagtaacca
      181 gattatcaaa gtgggatcaa accaggttga aaaggtgggg atcattcgtg cgctgacggt
      241 gggtgtggct taccagacga cggtaggcgg cattatgaat acctcggtgg cgttgttgca
      301 gtcctcacag gtagggctgc ataaatcact gatggtggga atgggctaca gcgtcaatgt
      361 agggaataac gtcaccttct cggtgggcaa gacgatgaag gaaaacaccg gacaaacagc
      421 agtttattct gccggtgagc atcttgaact ctgctgtggt aaggcaaggc tggtgctgac
      481 gaaggacgga agcatatttc tcaacggtac gcacattcat ctggaagggg agtcggatgt
      541 gaacggtgat gcgccagtga ttaactggaa ctgtggtgcc acacaacctg taccggatgc
      601 gcctgtgccg aaagatttac cccccggaat gccggatatg cggcaatttt gagtcaggtg
      661 caaaaaatta aaacggaact ggtgcagtga ccagatgaac catattaaaa gcagaaggaa
      721 ttgattcatg ggcggcggaa aaccggcggc gcggcagggc gacatgaccc gcaagggact
      781 ggatattgtg cagggttcag cgggtgtgct gataggtgcg ccgacgggag tggcctgttc
      841 ggtgtgtccg ggagggatta cctatgctaa cccggtgaac ccggtgctgg gtgcgaaggt
      901 gcttccgggc gagacggacc ttgcgctgcc ctgcccgctg ccgtttattc ttttccgcgc
      961 ctacagcagc taccggacca gaacacccgc gccggtgggg gtgtttggtc ccggctggaa
     1021 agcgcccttc gatatccgct tacagatacg cgatgaaggc ctgatactca acgacagcgg
     1081 cggcaggagc attcactttg agccgctgtt tcccggcgag ataagctaca gccgcagcga
     1141 gtcactctgg ctggcgcggg gcggagtggc ggcgcagcac agttcgcagc cgctaagcgc
     1201 gctctggcag gtgctgccgg aagatgttcg cctgagtccg catgtgtacc tggcgacaaa
     1261 cagccttcag gggccgtggt ggatactcag ctggccggag ccgcccgcat accgggtact
     1321 gacggtcgtg gtggatggct tcgggcgctc actgactttc caccgggcgg cggagggtga
     1381 cgtggcgggc gcggtgacgg gggtgacgga cggcgcgggc cgccgttttc acatggcgct
     1441 gagcacgcag gcgcagcggg cggaagcgtc ccgtaaacag cgcgcctctt ccttatcttc
     1501 cccggccagt ccccgttctg tttcctcttc tcaggt
//
LOCUS       DAZOYL010000472         1536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZOYL010000472 DAZOYL010000000
VERSION     DAZOYL010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.476"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /locus_tag="ACHUOY_004150"
     CDS             complement(<1..1012)
                     /locus_tag="ACHUOY_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR0684736.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGK"
     gene            1267..1513
                     /locus_tag="ACHUOY_004151"
                     /pseudo
     CDS             1267..1513
                     /locus_tag="ACHUOY_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          438 a          353 c          275 g          470 t
ORIGIN      
        1 tttttccccc aaaataaaca gacattggtg catcattata agctaatgct ttacccattt
       61 tacccgcagc taaatcaact gatgtggcag aaccattatc ttcaacaaat gcagttacac
      121 gaccaccaaa gtttgctaac tgaacatcct taccacccac ctgaactttt tcataacctg
      181 tagagttttg tttcaataat gtaaaaccgt tcgtgttggt attattatct tgaccaacaa
      241 aataagtctg cccttccacc tgtacttgat aaacttttgt tcctgcagta ccggtcgcat
      301 cagttgtatc ctttgtatct tgtacattac cggtaccaaa ataagtttca acatcagcat
      361 ttttataatt gctaccgaat gctttagcac ctcccacact accatcagtt gcatttacct
      421 tagttaagtc aggagcggct tcgctcttaa gtgcaaccgc agaggacatt ggaacttttc
      481 ctagtgctac gctaaagtta tcaagcccca aggttttagc atcaatttta tcaagggcaa
      541 tctcaatggt ttcaccatcg ttcgcaccta cttgaatttt catagtctga tcagaagcca
      601 gaacttttac accattaaat tgtgtctggt tagaaacacg atcaatttca ctaaggcgtt
      661 gtgtaatttc atcctgaatt gaattcaggt cggagtcaga gtttgtacca ttagtggcct
      721 gtactgacaa ttcacgaata cgctgtaagt tattgttgat ttcagaaagc gcaccttcag
      781 tggtctgcgc aacagaaata ccatcattcg cgttacggga agcctgagtc agacctttaa
      841 tatttgctgt aaaacggtta gcaatcgcct gacctgctgc gtcatcttta gcactgttaa
      901 tacgcaggcc agaagagaga cgttcaatgg cggagctcag agaagactga gatttgttca
      961 ggttattctg ggtcaacagc gacaggctgt ttgtattaat gacttgtgcc atgatctttt
     1021 ccttatcaat tacaacttga tgttattggg ctgttgccca cggtttctca ccgtacccct
     1081 tgtatcggca cctgaatttc gaactttaga aaattttagt ttctctcctg ttttttctta
     1141 acggctgaaa tatccgtaat atacaactct actctgcgca tcatcttcgt gaatcaacta
     1201 ttaaactttg cgcacaaatt gccgataacc cgcttatcta ctgttttcaa tcaaaaggaa
     1261 ttaggtatgg cttcatttac atctcttggc gttggttcga atctgccgct ggatacattg
     1321 ctaaacaacc tgaccatcgc tgaaaaaaaa cgcttaaacc caattactca gcaacagagt
     1381 gacaataccg cacgtctgac agcctatggc acattaaaaa tgtgcattag aaaaattcca
     1441 gactgcaaat actgcgttaa ataaagccga tctgttcaaa agcactaccg ttaccagtag
     1501 caatgaagac ctttgaggta gcctgagttt aacgga
//
LOCUS       DAZOYL010000473         1507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZOYL010000473 DAZOYL010000000
VERSION     DAZOYL010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.477"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1491
                     /locus_tag="ACHUOY_004152"
     CDS             <1..1491
                     /locus_tag="ACHUOY_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0684737.1"
                     /translation="AEQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVS
                     RRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAP
                     CCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERG
                     IAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEAL
                     HGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDR
                     KGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALT
                     EEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELA
                     KAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALL
                     YSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT          338 a          425 c          472 g          272 t
ORIGIN      
        1 gccgaacagg aagcgctgat ccacgccctg caggaaaagc ttagcaaccg ggagcgcgaa
       61 atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg cagtcgttcc
      121 gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct tcagaaagag
      181 agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc
      241 cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg
      301 gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga taccgccgaa
      361 cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga aaaacatgcc
      421 tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat cgagcggggt
      481 atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga gcacaccccg
      541 ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg ttcactgctg
      601 tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc gcttcatggc
      661 tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt gctgttgcca
      721 ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga ccgtaacgcc
      781 gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa aggcatccat
      841 ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata cgccgggttc
      901 aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca cgcccgccgt
      961 aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc gctgaaacgg
     1021 atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga acagcgcctt
     1081 gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg gctgcgtgaa
     1141 aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata cgccctgaac
     1201 cagtggccgg cgctgacgta ctatgcagat gatggctggg ctgaggcgga caataacatc
     1261 gctgaaaatg cgttgcggat ggtcagtctg ggccgcaaaa actacctgtt cttcggttcg
     1321 gatcatggag gagagcgggg agcgctgctg tacagcctga tcgggacgtg caaactgaac
     1381 ggagtggagc cagaaagcta cctccgctat gtccttgacg tcatagccga ctggccgata
     1441 aaccgggtcg gcgaactgct cccctggcgc gtagcactgc cgactgaata acacattccc
     1501 gtcaata
//
LOCUS       DAZOYL010000474         1471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZOYL010000474 DAZOYL010000000
VERSION     DAZOYL010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.478"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(161..814)
                     /gene="ribB"
                     /locus_tag="ACHUOY_004153"
     CDS             complement(161..814)
                     /gene="ribB"
                     /locus_tag="ACHUOY_004153"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR0684738.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(918..1066)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1188..>1471
                     /gene="ubiK"
                     /locus_tag="ACHUOY_004154"
     CDS             1188..>1471
                     /gene="ubiK"
                     /locus_tag="ACHUOY_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR0684739.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPT"
BASE COUNT          415 a          382 c          341 g          333 t
ORIGIN      
        1 tatatgattt tgatcataat gaaaaagaat gcctgatatt aatgaaattt tctcatagaa
       61 agagaagcgg aattaacatc ttgcatagca gggcaaaata aaaaccgaag ccagctacaa
      121 cttcggtttc ttgattaagg cagtaattaa gcagcggttt tcagctggct ttacgctcat
      181 gtgcctgacg gtatgccacc aggtcttcaa tagtcacgag cgccatattg tgtttattgg
      241 caaactcaat gcactctggt gcacgcgcca tcgtgccatc gtcattagtc agctcacaca
      301 gcacaccagc cggtttaaag cctgccagcg tcatcaaatc aatagtcgct tcagtatgac
      361 cgccacgcgt cagtacacca cctgcctgcg cgcgaagtgg gaaaacgtgg cccgggcgat
      421 tcagatcaga aggttttgcg ccatcggcaa tcgctgcgcg aacggtcgta atacgatcag
      481 cggcagaaac accggtagtc acaccttcag ccgcttcaat ggtcacggta aaaccggtgc
      541 cataggcgct ggtgttattt tctaccatca ttggcagatc aagttgttta cggcgatctt
      601 cagtgatgca caggcaaaca ataccgctac cgtggcgaat ggtcagcgcc atctgctcaa
      661 cggtcatggt ttctgccggg aagatcatat cgccttcgtt ttcacggtct tcatcatcaa
      721 gcaccattac accgcgtcct tcacgcagcg cagccagtgc attttcaaca cgttcgaaag
      781 gcgtaccaaa agaggaaagt agcgtctgat tcatggtaaa aaaacctcac taaaattatg
      841 gttaccagaa tcagggcagt cttaggagtg gcggcatata gccaaaataa cgtgagcggg
      901 tccatgcccg acagaatcgt tactctctcc catccggact ctaaccgtcg gccccggaat
      961 tacaccggat ctgctgtcct ttgagttcgc acccaaagcg ctcgcgggct ttcaactgag
     1021 ttgatttacc gccggtgggg aatttcgccc cgccctgaga ataagcggat tcactataac
     1081 gctaatgatt agcggcagca acgcatagct tcacataatt ctggtttatg acttaccctt
     1141 atcgcactac aatggtactc aacacctatc agtacaggga aactacaatg attgacccga
     1201 aaaaaattga gcaaatcgct cgccaggttc acgaatcaat gcctaaagga atcagggagt
     1261 tcggggaaga tgtggagaaa aaaatccgcc aaaccctaca agctcagctg acgcgcctcg
     1321 accttgtaag ccgtgaagag ttcgacgtcc aaacgcaagt cctgttacgg actcgtgaga
     1381 aactggcgcg gcttgaacaa agaatgagcg aactggaaaa tcgcagtact gagattaaaa
     1441 agcagccaga tccagagact ctccccccaa c
//
LOCUS       DAZOYL010000475         1468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZOYL010000475 DAZOYL010000000
VERSION     DAZOYL010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.479"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..924)
                     /gene="yfdV"
                     /locus_tag="ACHUOY_004155"
     CDS             complement(<1..924)
                     /gene="yfdV"
                     /locus_tag="ACHUOY_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR0684740.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIY"
     gene            complement(994..>1468)
                     /locus_tag="ACHUOY_004156"
     CDS             complement(994..>1468)
                     /locus_tag="ACHUOY_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFR0684741.1"
                     /translation="MYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAFG
                     FSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDAF
                     RGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
BASE COUNT          459 a          361 c          313 g          335 t
ORIGIN      
        1 ataaatccac aagggagccg tgacgacaaa gcccagtacg ctcacagcca atgacgctgt
       61 accagtacgg gtgtagacat taaaccggct ggcaataatg attccggaga atgccggcgg
      121 taatgcgcct gccagtacca tcatctgcaa atgttcgctg ttcaaatgac atgccatacc
      181 aacgagaagc agcgccagtg gcatcagaat caacttcagg aaggtgttat aagcaatttc
      241 ggcactgaac tcgaatttat gtgcagccag agtcaacccc gcagcgaata ccgctacccc
      301 tgagttagcc ttcgcaatca gattaaaggt cgggtcccat gctgccggaa tttttacccc
      361 aaccagcacc aggatcgttg ccagaacagg tgcccatact actggctcct ttgccgcaga
      421 aattaatgcg ctcagattac tattcttctt accatccgct cctgaggaag gattcagcaa
      481 atacagacca ataggaatag taattgcgtt aacaataata gaaataattg ctaccactaa
      541 acctgttgat acggaatcac cataaatagg atcgagaact gcaaacccca ggaatccaat
      601 ggtaggtgaa cccgcaatta atgcacatac agcggcttct gcatgggtac gtttaaaaaa
      661 tttgtagcaa ccaaaccaag agaaaaagaa acatccgaca ataaccacaa gtgatacaag
      721 ggtcagacga gtgtccgcaa aaatcatttc cctgtttgcc cgggtaatag aaacaaatag
      781 agccgcagga agagcgtagt ttaataccag tttattaaat gcgcgggctt gatcttctga
      841 aaaagtttct cgtctgccgc taaaataacc caacagcatg attacgataa taggcaataa
      901 atcgccaata aaaaatgtta gcataatagt atttccgata agggtcccct caccatcgca
      961 taatgagttg gcaagaggag acgatgtcag tatttaatta ccagcgactt gttttgggtt
     1021 aagtttggta atatggccac tttcagttcc tgcagcaggg tcgatgacca cattaataat
     1081 ggtcggtttg cgcgactgaa taccggtggt taaagcatga cgaagttcat ctgtcgtggt
     1141 gacgttatag ccaacgccac gaaacgcatc cattaatttg tcataccttg catggtgcaa
     1201 cagatccgtt ggtgatggtg caccagcgcc actgagatca acaccgtctc ctctgtagat
     1261 gccgccatta ttaaaaataa cgatcgtcac cggcaggtta tatcgacaaa tcgtttcaat
     1321 ttccatccca ctgaaaccaa aagcactatc accttcaatg gcgacaaccg gagaaccaga
     1381 agtcacgcta gcaccgatgg catagcccat accgatgccc atgacacccc aggtaccaca
     1441 atccagacga cgacgtggtt tatacata
//
LOCUS       DAZOYL010000476         1457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZOYL010000476 DAZOYL010000000
VERSION     DAZOYL010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.480"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="ACHUOY_004157"
     CDS             complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="ACHUOY_004157"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0684742.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHAAIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1090..>1457)
                     /gene="cadB"
                     /locus_tag="ACHUOY_004158"
     CDS             complement(1090..>1457)
                     /gene="cadB"
                     /locus_tag="ACHUOY_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0684743.1"
                     /translation="LLAAVKMTALIILITLMNSAGGKASDLFGELTGIAVLLTMLPYF
                     YSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYAR
                     KMHERQSHSMDNHTASNAH"
BASE COUNT          406 a          320 c          364 g          367 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg cagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt
      961 tcttctttaa aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct
     1021 ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa
     1081 tacttttaat taatgtgcgt tagacgcggt gtggttatcc attgagtggc tctggcgctc
     1141 gtgcattttg cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag
     1201 ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac acacaaccca gtacagagca
     1261 gatcaggctg acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga
     1321 gtagaagtac ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc
     1381 agatgcttta ccaccggcag agttcatcag agtgatgagg attatcaggg cagtcatttt
     1441 cactgcagcc agcagca
//
LOCUS       DAZOYL010000477         1450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZOYL010000477 DAZOYL010000000
VERSION     DAZOYL010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.481"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..223
                     /locus_tag="ACHUOY_004159"
     CDS             <1..223
                     /locus_tag="ACHUOY_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HFR0684744.1"
                     /translation="VRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSLEQV
                     MEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            220..1302
                     /locus_tag="ACHUOY_004160"
     CDS             220..1302
                     /locus_tag="ACHUOY_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR0684745.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1343..>1450)
                     /locus_tag="ACHUOY_004161"
     CDS             complement(1343..>1450)
                     /locus_tag="ACHUOY_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0684746.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
BASE COUNT          383 a          275 c          370 g          422 t
ORIGIN      
        1 ggttcgccct gatgttattg atatgctact taattcgcat gttgtcgatc gggcgttcag
       61 gtgtataagc ggaagtgttg ctgtttcggc ctatgaattg aattcaatcc cgttgccttc
      121 tctggagcag gtgatggaga ttcaatcgtt aattgatgct ggcgtccaca gacggattat
      181 tgagagaact atcgctggtt tctatggagt gatgattgca tgaatctgcc tgttgttccc
      241 ccattgaatg ttatccggga aagattacaa ttaattttcc ctgaagggac tgagaatcgt
      301 ggatatttaa ttcgggaaat agctgcaaaa acaatttttg tcatgtttta tgctggtgcg
      361 gttgaggggc tggggcgatg gataaggccc agccaggttg taagtatggg agattcgcag
      421 gctgctctta ctgatgatgc gtccagagaa agctgggtaa agatgacttt ctctaaacaa
      481 aagataaggc ctgctgatgc atggtatgcg gaaaatacgc gtgagccaat acgtgatgaa
      541 actataaaaa atggcctaat accttgcaat gcaattgttg agcgtaaagg tatacccacc
      601 acatcctctc gtcctcgtta cagtttgaat cgctcttttg cagcactctt tgatgccggg
      661 cttgaaggag attcgctgct gagtgcaata agttcctggc aagaaaatca tttgaacaaa
      721 gccgctcttg ctaggttgcg tttattgaaa tcaggggcag tgattgctgc tgatgccgta
      781 gtcgtcactt tcccaaatgg agagaagaga actttagctc cgggcccgtc aagtacaata
      841 gctaaggccg tcatcgaagt ttttgctcct aattttctta agaatccaac tgtgctctgg
      901 ttgtctgaat ccgggaataa agttgttgct caggatgatg tattggcaaa agcattgggg
      961 ctgaatattg atgcctcaaa agcacttcca gatattattt tgattgattt gggagaggac
     1021 cgaacgggtt cggacatgct tgtagttttc actgaagtag ttgcgacaga tgggcctata
     1081 aacagggaac gaaaagttac tctcactcat ttggctattg acgctgggtt cagcgagaaa
     1141 aatcttgcat tccttacggc atttatggac agaagtgctc tgccttttaa aaaggcaata
     1201 ccagaattgg cgtggggatc ttatgcatgg tttgcttctg aaccggagca cttaattgat
     1261 cttcgggatg gttatccggt aaagatcagc cagagaaaat gaccaaagtt tgatatccgc
     1321 cacgccctga aggatggggg ttttacggtg cactggataa aaaatcggtt tcatagattt
     1381 gcccaccgtg gtatacgcct gcgataaaaa ctttattgtc atcaacggca aaagcaataa
     1441 ttgttctgtg
//
LOCUS       DAZOYL010000478         1436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZOYL010000478 DAZOYL010000000
VERSION     DAZOYL010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.482"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            89..>1436
                     /gene="istA"
                     /locus_tag="ACHUOY_004162"
     CDS             89..>1436
                     /gene="istA"
                     /locus_tag="ACHUOY_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR0684747.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKY"
BASE COUNT          367 a          401 c          364 g          304 t
ORIGIN      
        1 ggaattaact cttttcacca cagtctccat tgccgtgttt aaaccagtgg agacgtcaat
       61 gtccaaaaag agaaaatcca ggagcactat ggaaacttgt tttaagatcc ttcagctcaa
      121 gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac
      181 cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga
      241 tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga
      301 gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg
      361 ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca
      421 cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca
      481 tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc
      541 ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta
      601 tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg
      661 ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc
      721 agtgaaaaaa gccgatcgct atgagcctgt catcaacgac agttatcagg cgctggctga
      781 gcactatggc acggttatta tcccggcccg cccgcgcaaa cctaaagata aaccaaaagc
      841 agaaaacggc gtcctcatcg tcgagcgatg gcttcttgcc cgcatccgta atgaaacctt
      901 ccacacgctc agggcgctga atgcacgcct tcgggagttg ctgacggaca tgaataaccg
      961 accaatgaag ggatacggta atcaaacccg cgcagaacgc ttccgcatgc tggatgcacc
     1021 agcactctct ccgctgcctc tggagcctta tgaatacacg gaatacaaag ccgtgaaggt
     1081 tggccctgac taccacgttg aatatgcccg tcactggtac tccgttccac acgaactggt
     1141 cggtcagcgt ctgtcactga aggtggggca gtcggtggta cagctctggc acaaggggca
     1201 gtgtgtggcg caacatccgc gcagcacaca tgaatataaa cacacaacca atccgcttca
     1261 catgccagaa cgccaccggc ggcatgggac atggacaccg gaacgcctta tcgagcaggg
     1321 aaaccgtaca ggtccgtcca caggccgggt ggttgaatcc atgctgaaag ccaaacccca
     1381 tccggagctg gcttaccgtg cagtcctggg tctgctggca cttcagaaaa aatacg
//
LOCUS       DAZOYL010000479         1431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZOYL010000479 DAZOYL010000000
VERSION     DAZOYL010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.483"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHUOY_004163"
     CDS             <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHUOY_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR0684748.1"
                     /translation="SQEKILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLE
                     IAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDV
                     PLNMIRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHUOY_004164"
     CDS             464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHUOY_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0684749.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          373 t
ORIGIN      
        1 ttcgcaagaa aaaatattac gcattattca caccaaaggt gtgtcattgc ctgatgatcg
       61 cgagtcagtc ttctggctga atattaaaaa tattccgcca tcagcgagca acaaagcgac
      121 gaactcgctg gaaatagcag taaaaacgcg tatcaagctg ttctggcgac cagcaaatat
      181 ccgccttatt cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag ggcgaaatct
      241 gatagcagag aatcccaacc caattcatat cagtgtgatg gatgtcatcg ttgatggcca
      301 tgatgtgccc ctcaatatga tccgtccttt tgaaacgctc acgttacctt tacctgctaa
      361 ttctgcggga ggccaaatga cctggcgatt tattaatgat tacggcgcag tcagtgaccc
      421 gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga cgaccagaat
      481 agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga tggccgaggt
      541 cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg atattcgcca
      601 gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt ttatcaacgg
      661 taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg ctgatctgca
      721 cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag ttgatgcgct
      781 caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac gtattgaagg
      841 ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc cccaaatcta
      901 catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg ggattaacgc
      961 gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt atggttcaca
     1021 agagacagat accagttatc tcaatctgcg caatggactg aatattggac cgtggcggct
     1081 acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact ccattagtac
     1141 ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg atacgtggac
     1201 ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata ctgataacga
     1261 tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg caaagtccaa
     1321 cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtctgccg ttccacaagg
     1381 tgcttttgag atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg a
//
LOCUS       DAZOYL010000480         1428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZOYL010000480 DAZOYL010000000
VERSION     DAZOYL010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.484"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUOY_004165"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUOY_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0684750.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUOY_004166"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUOY_004166"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR0684751.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1428)
                     /locus_tag="ACHUOY_004167"
     CDS             complement(822..>1428)
                     /locus_tag="ACHUOY_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0684752.1"
                     /translation="KGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFC
                     FGKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGAD
                     EEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVP
                     GAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          324 a          397 c          396 g          311 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgccttta
//
LOCUS       DAZOYL010000481         1424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZOYL010000481 DAZOYL010000000
VERSION     DAZOYL010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.485"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            38..442
                     /gene="stpA"
                     /locus_tag="ACHUOY_004168"
     CDS             38..442
                     /gene="stpA"
                     /locus_tag="ACHUOY_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR0684753.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(489..1013)
                     /gene="ygaP"
                     /locus_tag="ACHUOY_004169"
     CDS             complement(489..1013)
                     /gene="ygaP"
                     /locus_tag="ACHUOY_004169"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR0684754.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1023..1322)
                     /locus_tag="ACHUOY_004170"
     CDS             complement(1023..1322)
                     /locus_tag="ACHUOY_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0684755.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          386 a          336 c          337 g          365 t
ORIGIN      
        1 tacttttttg ttttggcgtt aaaaggtttt ctttattatg tccgtaatgt tacaaagttt
       61 aaataacatt cgcaccctcc gtgcgatggc tcgcgaattc tccattgacg ttcttgaaga
      121 aatgctcgaa aaattcaggg ttgtcactaa agaaagacgt gaagaagaag aacagcagca
      181 gcgtgaactg gctgagcgtc aggaaaaaat tagcacctgg ctggagctga tgaaggctga
      241 cggaattaac ccggaagagt tattgggtaa tagctctgct gctgcaccac gcgctggtaa
      301 aaaacgccag ccgcgtccgg cgaaatataa attcaccgat gttaacggtg aaactaaaac
      361 ctggaccggt cagggccgta caccgaagcc aattgctcag gcgctggcag aaggtaaatc
      421 tctcgacgat ttcctgatct aattactgag gccggatgtc gctgctaggg cgcgtccggc
      481 gtcgcttctc aagctcgttg gttccacggc atcttatcta acaaccttgc cattccacaa
      541 aaaccgctga ttcctgcaaa cagtaacccg gcaccaacaa agccacttaa taagaagaaa
      601 ccgctattta cggtataacc cagtacaacg ccgattaata ttaaaccacc cgcagcgatc
      661 tgcacctggc gcattaacgg caagggttga gatttattta ccgctactgg caatccagct
      721 cttttccagc catcaatccc atcttcgagt aaaaatattt ctgcgggggc ggcaatcgct
      781 gctaatttat cggcgttatt actggtgcgt ttacctgcct ggcagtggaa tataatttgc
      841 tcgcgacgta atttaggtgg aagacctgac tgttccagca cggataatgg agccagatct
      901 gcttcaggaa tatgttcacg aagatattca tcagcgtcac gaatatcgat taactttgcg
      961 ccgcgtgcga ttaattcttg tgcatcatgc ggcgaaatgg ttgtcaaagc catagtgact
     1021 ccttacggac aatagacatt tttcagggtg gcgataatgg catttaccgc ctcattttta
     1081 atggaatata gaatgcgttg ggcatcccgt tggctgtcga taagcccttc gtcccgcata
     1141 cgagcgagat gctgtgatgt cgcagaggca ctcagtccgg taatgcgcgt cagctctccc
     1201 gcgctggtgc cgggggaacc gctaagcatg cacagaatca gcaatcgttt aggatggctc
     1261 attgctttca ataaggccgc tgcctgttcg gcactggcct gtaattgcgc gagttcagtc
     1321 atatttattt aagtattttc taaattaagt aagctctaaa ctaaaaatgc aacatatacc
     1381 agcctcagca gcgtaaatga gagtaaaagc gtaagctgaa actg
//
LOCUS       DAZOYL010000482         1423 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZOYL010000482 DAZOYL010000000
VERSION     DAZOYL010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.486"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..656)
                     /locus_tag="ACHUOY_004171"
     CDS             complement(<1..656)
                     /locus_tag="ACHUOY_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFR0684756.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
     gene            complement(820..915)
                     /gene="ymiC"
                     /locus_tag="ACHUOY_004172"
     CDS             complement(820..915)
                     /gene="ymiC"
                     /locus_tag="ACHUOY_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR0684757.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1029..1196)
                     /gene="yciX"
                     /locus_tag="ACHUOY_004173"
     CDS             complement(1029..1196)
                     /gene="yciX"
                     /locus_tag="ACHUOY_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR0684758.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1199..1339)
                     /locus_tag="ACHUOY_004174"
     CDS             complement(1199..1339)
                     /locus_tag="ACHUOY_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR0684759.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          379 a          347 c          331 g          366 t
ORIGIN      
        1 ttgatcatag tggtgtgcga gtcagtacca acgagtgtat ccggataagc aatccattca
       61 ccgtcctgca attcactcca cactgctttg ccgagatatt cgaggttaac ctgatggcaa
      121 atgcctgtgc ctggcggcac gacgctaaac cgactgaacg cttgctttcc ccatttcagg
      181 aacacataac gttcgtggtt gcgctccatt tccaggcgta cgttttcttc aaatgcctca
      241 tcatcaccaa aacgatcgac ggtcaccgag tggtcaatga ccaggtcgac cggtgagagc
      301 gggttaacct ttgcagtatc gccgccgagg cgtttaaccg cttcgcgcat tgccgccaga
      361 tcaacaacgg caggtacgcc ggtaaagtcc tgcatcaaca cccttgccgg gcggtaggca
      421 atttcacggt cagcatgggc atttttcagc catcctgcca gcgcgtggat atcctcttcg
      481 gtaaccgagt taccatcctg ccagcgcagc aggttttcga gcaaaacttt gagtgacttg
      541 ggtagacggg tgatatcgcc cagtgattta gcagcaagcg gcaggctgta gtagtggtaa
      601 gttttatctt tggcctgcaa cgtgtcctta ctggcttctc gtagggttga cgacatagct
      661 cctccttaat gacagggttg cgaagccctg attctcttca gggtgaatat taaagataac
      721 acaaacatcg cgtaacgatt tgataacaac ccaaattgat aaaagaggag aaaccttgtg
      781 gcaaagcaaa tgacaacccc gccgcagcgg ggtcaaggat cagtgagtaa tgatccagag
      841 gagttcggcc cagaagagca tcgacagaga aaacgcgccc atccaggacc aatatttcat
      901 atttgtgttg atcataggct acacccttaa cggttattat tttgtgttga gataactcaa
      961 gctgttattg ctgcggtgta ttaaaagccc tttaattcat taaaaatggg taataagaat
     1021 gatgataatt attgtttcgg ctgatcatct tcagcaaaca gttctataaa ggcttgctgc
     1081 tgcgatgtta gttgccagtc gtctggaata acgacggaac attcccgttt cgtttctgtt
     1141 tcattgtcac tatgctggca taatggtgac gactccagtt gctcctgacc gaccatagtt
     1201 atccccacac tttccaaatc agcagtgcga caaccaccca gaaaagtgca gaaccaagaa
     1261 aaaccgccag ccaggcttta cgttttaatt caggatcgcg gcggggttcc tgatttccag
     1321 aaggcattgc taacctcata taatcgccat cacttatcag caagacgttg aacgattggt
     1381 gtaattaatc atacgatgag acaaatccta aagaaacttt agt
//
LOCUS       DAZOYL010000483         1407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZOYL010000483 DAZOYL010000000
VERSION     DAZOYL010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.487"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            448..903
                     /gene="csgB"
                     /locus_tag="ACHUOY_004175"
     CDS             448..903
                     /gene="csgB"
                     /locus_tag="ACHUOY_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR0684760.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            944..1396
                     /gene="csgA"
                     /locus_tag="ACHUOY_004176"
     CDS             944..1396
                     /gene="csgA"
                     /locus_tag="ACHUOY_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR0684761.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          424 a          272 c          330 g          381 t
ORIGIN      
        1 caaatggtga taacttacta ataatgcata taaaaaatat ttcggtgtag tcctttcgtc
       61 atgtaaaaag ttgttgtttt ttctccacac ctccgtggac aaatttttac tccaaaaaga
      121 cgaggtttgt cacggcttgt gcgcaagaca tatcgcagca atcagcgatg ggcaagaaga
      181 atgactgtct ggtgattttt gatagcggaa acggagattt aaaagaaaac aaaatatttt
      241 ttgcgtagat aacagcgtat ttacgtggct tttaatactt tggtatgaac aaaaaaagaa
      301 aaatacaacg cgcgggtgag ttattaaaaa tatttccgca gacatacttt ccatcgtaac
      361 gcagcgttaa caaaatacag gttgcgttaa caaccaagtt gaaatgattt aatttcttaa
      421 atgtacgacc aggtccaggg tgacaacatg aaaaacaaat tgttatttat gatgttaaca
      481 atactgggtg cgcctgggat tgcagccgca gcaggttatg atttagctaa ttcagaatat
      541 aacttcgcgg taaatgaatt gagtaagtct tcatttaatc aggcagccat aattggtcaa
      601 gctgggacta ataatagtac tcagttacgg cagggaggct caaaactttt ggcggttgtt
      661 gcgcaagaag gtagtagcaa ccgggcaaag attgaccaga caggagatta taaccttgca
      721 tatattgatc aggcgggcag tgccaacgat gccagtattt cgcaaggtgc ttatggtaat
      781 actgcgatga ttatccagaa aggttctggt aataaagcaa atattacaca gtatggtact
      841 caaaaaacgg caattgtagt gcagagacag tcgcaaatgg ctattcgcgt gacacaacgt
      901 taatttccat tcgactttta aatcaatccg atgggggttt tacatgaaac ttttaaaagt
      961 agcagcaatt gcagcaatcg tattctccgg tagcgctctg gcaggtgttg ttcctcagta
     1021 cggcggcggt aaccacggtg gtggcggtaa taatagcggc ccaaattctg agctgaacat
     1081 ttaccagtac ggtggcggta actctgcact tgttctgcaa actgatgccc gtaactctga
     1141 cttgactatt acccagcatg gcggcggtaa tggtgcagat gttggtcagg gctcggatga
     1201 cagctcaatc gatctgaccc aacgtggctt cggtaacagc gctactcttg atcagtggaa
     1261 cggcaaaaat tctgaaatga cggttaaaca gttcggtggt ggcaacggtg ctgcagttga
     1321 ccagactgca tctaactcct ccgtcaacgt gactcaggtt ggctttggta acaacgcgac
     1381 cgctcatcag tactaataca tcatttg
//
LOCUS       DAZOYL010000484         1392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZOYL010000484 DAZOYL010000000
VERSION     DAZOYL010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.488"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHUOY_004177"
     CDS             <1..459
                     /locus_tag="ACHUOY_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0684762.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHUOY_004178"
     CDS             477..1115
                     /locus_tag="ACHUOY_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0684763.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZOYL010000485         1386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZOYL010000485 DAZOYL010000000
VERSION     DAZOYL010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.489"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1099
                     /locus_tag="ACHUOY_004179"
     CDS             <1..1099
                     /locus_tag="ACHUOY_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0684764.1"
                     /translation="RQNENHEAGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQ
                     SLKDLERAYKNFFRKRAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRN
                     SRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTV
                     FEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTT
                     AVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEY
                     KQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNIL
                     AAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(1139..>1386)
                     /locus_tag="ACHUOY_004180"
     CDS             complement(1139..>1386)
                     /locus_tag="ACHUOY_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR0684765.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
BASE COUNT          381 a          369 c          359 g          277 t
ORIGIN      
        1 acgtcagaat gagaatcatg aggccggtaa taaatacatc ccttacggga aaatggcttc
       61 ctggctggtt gagtggaaaa atgccactga aacgcaatgg cttaaagatt ctccctcaca
      121 gccattgcaa cagtcactga aagaccttga gcgggcttac aaaaacttct tccggaagcg
      181 ggctgctttt ccccgattca aaaagcgggg acagaatgat gtattccgct acccgcaggg
      241 cgttaagctc gatcaggaaa acagccgtat ttttctgccg aaactgggct ggatgcgcta
      301 ccggaatagc cgtcaggtca cgggtgttgt gaaaaatgtc actgtcagcc agtcctgcgg
      361 tacgtggtac atcagtattc agacagaaag tgaagtatcc actccggctc acccttcagc
      421 atcaatggtc gggctggatg ctggcgtggc taaactcgcc acgctgtcag atggcacagt
      481 ctttgagcct gtaaacagtt ttcagaaaaa ccagaagacg ctggcgagac ttcagcgaca
      541 gttaagccgc aaggtcaaat tcagcaacaa ctggcagaag cagaaacgca aaatacagcg
      601 actgcattcc tgtatcgcaa atatccgcag ggactacctt cacaaagtca caacggccgt
      661 cagcaaaaac cacgcaatga tagtcattga ggatttgaag gtcagcaaca tgtcaaagtc
      721 agcagcgggt acggtcagcc agccggggcg caatgtccgg gcaaaatcag gtttaaaccg
      781 ttcgatactg gatcagggct ggtatgaaat gcaccgccag cttgagtaca agcagctctg
      841 gcgtggcggt caggtgcttg ctgttccgcc agcgtataca agccagcgtt gcgcgtactg
      901 tggtcataca gcgaaagaga accgcctgtc acaaagtaaa ttcagatgcc aggtatgtgg
      961 atatacagcg aacgccgatg taaatggtgc tcgtaacatt ttagcggcgg ggcacgccgt
     1021 tcttgcctgt ggagagatgg tgcagtcagg ccgcccgttg aagcaggaac ccaccgaaat
     1081 gattcaggcg acagcctgaa cgtagcaggg atccacgtcc ttcagggcgt ggaggatgtc
     1141 aatatcggtt tctgtcagcg attaaattca acaccacgta acaaacgcct ccggcaatta
     1201 atccccagaa cgcagaacca atcccgacca gcgtcaatcc acttgccgtt accagaaatg
     1261 ccaccaccgc cgcgtcgcgc tcacgctcac gctcattatg cagcgcctga tacaaactgc
     1321 cgccaatggt actcaacagc gccagacctg ccagcatctg gatccaactt acgggcaggg
     1381 cagcca
//
LOCUS       DAZOYL010000486         1382 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZOYL010000486 DAZOYL010000000
VERSION     DAZOYL010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.490"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            311..1120
                     /locus_tag="ACHUOY_004181"
                     /pseudo
     CDS             311..1120
                     /locus_tag="ACHUOY_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1317..>1382
                     /locus_tag="ACHUOY_004182"
     CDS             1317..>1382
                     /locus_tag="ACHUOY_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684766.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGV"
BASE COUNT          398 a          241 c          364 g          379 t
ORIGIN      
        1 gatctgattt caggcgttgg gtgtggatca ctattgcacc gttcgtgaca cttgttgact
       61 gcctgtgcgg catctggtat caaggtggcg tgaagccggt tcaggttctc atgcggaacg
      121 ttggaaccta tatccccccg gtaccaaagg cgaaatgtaa gtgacaaaat gcaatgaaca
      181 cgttctccgt taactgagga gcatggtgat aaataaaggt aaatgcagaa ggtgtagagt
      241 ctcgcagatt tctgacatca tagagcctaa cattagatgc ataacgctac agattgtaga
      301 atctctacag gtgaaatata cactggttgg cgtttatgtc gcaaaacatg tcattcaggt
      361 tcatctcacc aataaggata tgagtgaagt ggaggataag tgagtctgct gtcagagttt
      421 tctggtgtat gtcagtaact gcgaaccatg tctgcttgat atgggagctt atggaggctc
      481 tctgtacaag gttagggagc taacaaagca gggataaaaa gtccgcctga tgcaggcacg
      541 gttcgttaaa tcttttgcca ttggtaacaa aatggtgcga tgaatgttca ggctatcttt
      601 atcttgatgg tggttcagct gtttggtaaa gaaatcgctg taacaataga agaactgcag
      661 gcagtactgg tcccacatcg tttgctcagt caactggtga agttccgtag cgcgtaaatt
      721 aatgctttgc atgaaacgtt actggagtgg atgaaagaat acctgcatat atcattactg
      781 cgttggatta ccagtacaac agattgaatg aactggacat gttgatagac ggcattgaaa
      841 agcagcttat aagcatagtg cggcagagtt aattggagag cacttcgcct tctgagtatt
      901 aagtaagcat ggtggtacct gcggatacta tctattcact gtgctcggtc ggcagcctta
      961 ctgaccaagg agttggggga gtcgataaca aagttgaaaa aacagctctc aaccaaggtg
     1021 gttattgtaa caatagcaaa taagctggtg tgacctgaat cacaataacc gattatggac
     1081 gtaaatacga caaaaatatg tcagtatcag gcggtattaa ttgctgatgc tgatggacat
     1141 taactcttaa taaaagggtg gatgctgaaa ggttgttatg gtgacggacg tgatgacgac
     1201 ggaaaagagt atgaggtttt ggatttaaag ttcgttcggg gaataaggac ccggtcagct
     1261 aattctcata gtagttcgca ttgctggatg caatactgtg ctgcaattac tcgtcgatgt
     1321 cggaacaacg gaagccttgc aaacggggat gtattcatac aggtgttatg ttatatgggg
     1381 tt
//
LOCUS       DAZOYL010000487         1380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZOYL010000487 DAZOYL010000000
VERSION     DAZOYL010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.491"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            78..>1380
                     /gene="norR"
                     /locus_tag="ACHUOY_004183"
     CDS             78..>1380
                     /gene="norR"
                     /locus_tag="ACHUOY_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR0684767.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHF"
BASE COUNT          285 a          317 c          448 g          330 t
ORIGIN      
        1 taatgagtag tcaaaatgac tatcaatcaa atggtcgata tgacaatatc tatagtcaaa
       61 ttgacagtga ggcaaagatg agtttttccg ttgatgtgct ggcgaatatc gccatcgaat
      121 tgcagcgtgg gattggtcat caggatcgtt ttcagcgcct gatcaccacg ctacgtcagg
      181 tgctggagtg cgatgcgtct gcgttgctac gttacgattc gcggcagttt attccgcttg
      241 ccatcgacgg tctggcaaag gatgtactcg gtagacgctt tgcgctggaa gggcatccac
      301 ggctggaagc gattgcccgc gccggggatg tggtgcgctt tcccgcagac agcgaattgc
      361 ccgatcccta tgacggtttg attcctgggc aggagagtct gaaggttcac gcctgcgttg
      421 gtctgccatt gtttgccggg caaaacctga tcggcgcact gacgctcgac gggatgcagc
      481 ccgatcagtt cgatgttttc agcgacgaag agctacggct gattgctgcg ctggcggcgg
      541 gagcgttaag caatgcgttg ctgattgaac aactggaaag ccagaatatg atgccaggcg
      601 atgccacgcc gtttgaagcg gtgaaacaga cgcagatgat tggcttgtcc cctggcatga
      661 cgcaactgaa aaaagagatt gagattgtgg cggcgtccga tctcaacgtc ctgatcagcg
      721 gtgagacggg aaccggtaag gagctggtgg cgaaagcgat tcatgaagcc tcgccacggg
      781 cggtgaatcc gctggtctat ctcaactgtg ctgcactgcc ggaaagtgtg gcggaaagtg
      841 agttgttcgg gcatgtgaaa ggagcgttta ctggcgctat cagtaaccgc agcgggaagt
      901 ttgaaatggc ggataacggc actctgtttc tggatgagat cggcgagttg tcgttggcat
      961 tgcaggccaa gctgctgagg gtgttgcagt atggcgatat tcagcgcgtt ggcgatgacc
     1021 gtagtttgcg ggtcgatgtg cgcgtgctgg cggcgactaa ccgcgactta cgcgaagagg
     1081 tgatggcagg gcgatttcgc gctgacttgt ttcatcgcct gagcgtgttt ccactttcgg
     1141 tgccgccgct gcgtgagcgg ggcgatgatg tcattctgct ggcggggtat ttctgcgagc
     1201 agtgtcgttt gcggctgggg ctctcccgcg tggtattaag tgccggagcg cgaaatttac
     1261 tgcaacacta tcgttttccg gggaacgtgc gcgaactgga acatgctatt catcgggcgg
     1321 tagtgctggc gagagccacc cgcaacggcg atgaagtgat tcttgaggcg caacattttg
//
LOCUS       DAZOYL010000488         1365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZOYL010000488 DAZOYL010000000
VERSION     DAZOYL010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.492"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            95..727
                     /locus_tag="ACHUOY_004184"
     CDS             95..727
                     /locus_tag="ACHUOY_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0684768.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1061..>1365
                     /locus_tag="ACHUOY_004185"
     CDS             1061..>1365
                     /locus_tag="ACHUOY_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0684769.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT          446 a          248 c          256 g          415 t
ORIGIN      
        1 atttttccca gcaaaattac aaaatcaaga tgtattgaca tcatcaatca ccaatgaaat
       61 tatgcaatta tatacggata gggaggttct taacatgggg ctttgtagtc gttataaaag
      121 tcttacatgc aatagttgct caatgcattg ccaaataatg ccagaagagt caccgcgttt
      181 gcagtattgc gcaaactcgt gtttttgtat gtggcccgaa gaaagttcat attttaatcg
      241 tggtgtggtg gaaggtatct taacaaaaaa ccacaatgcg agattaagcg gatatatttt
      301 tgtggatttt tcagtaagtt ttttacgtct attccttgaa aaagactgga ttgactatct
      361 tgcaagtact gacatgggga tagttttagt cagcgaccgc aatatgcaat cgttagctaa
      421 ctactggcga aaacacaaca ccgcaatatc tgctgtcata tataatgatg atggtcttga
      481 tgttgccaat gaaaagatca gacaactatt tattggtcgt tatttatcat ttaccagagg
      541 taacacatta actcagatgg aattcaccat aatggggtat atggtttctg gttataatcc
      601 atatcagatt gctgaagtgc tggatatgga tatccgtagc atctacgcgt acaagcaacg
      661 aatcgaaaag agaatgggtg gtaaaataaa cgaattattt attcgttcac attcggtcca
      721 acattgatac tcaacgacca gccagaatca tactctgttc atacgggcat ctattgaaat
      781 gcccgtattt tataaataag cagtaacccc aagagtaaac aacatagtat ttaaaaccat
      841 cacacgaaaa attcagaagc attacgaaat atgaatttac aaacaatagt ggcataaatg
      901 ttaaccatgt taatttacgt aaagttttac gttgcaacat taaagcctca tttcaatcat
      961 catgataaat ataaaattaa tatatattta tgccgtaaat accaatatac ttagcaaact
     1021 atgtgatctc catttcgatt gatttagtgt ttattgacgt atgtactgga ttattaacga
     1081 taatatcgag ttctggcctg agcaccgaaa attaatatcg gtacataacg ccgatcttaa
     1141 cgtcgttctg acaacgccag ccagtcgatg tttatcactt ctacttgaag cttttcctga
     1201 tgtggttgca caacaagatt ttttcaccag agtctgggaa gaagaaggta tgcgtgtgcc
     1261 tactaacacg ttatatcaga acatatccat tatcagacgc ggatttcgcg ctgttggtga
     1321 tactacccac tcgctaattg caaccgtgcc gagagaggat tcaag
//
LOCUS       DAZOYL010000489         1357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZOYL010000489 DAZOYL010000000
VERSION     DAZOYL010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.493"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..1357
                     /gene="espL1"
                     /locus_tag="ACHUOY_004186"
                     /pseudo
     CDS             1..1357
                     /gene="espL1"
                     /locus_tag="ACHUOY_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
BASE COUNT          424 a          286 c          260 g          387 t
ORIGIN      
        1 gcccttgatt cattgagaaa tggaaaaggc aaagtcaacc tgattaaaca ctactcctcc
       61 gttgaatcca tacagcagca tgtcccctta gtccgggacg cggagttcag agcattactt
      121 cgccatcctc ctgcagggag tcgcgttatc gcgagtaagg attttggctt cgctttagat
      181 attttctttt gtcgaatgat ggcaaacaat gtcagtcata tgtccgcgat tttatatata
      241 gacaatcata ctttgtcagt aaggctacga ataaagcagt cagtgtatgg acaattaaat
      301 tatgttgtgt ctgtttacga cccgaacgat accaacgttg ccgtcagagg cacccacagg
      361 gcagcacggg gctttctctc gcttgataag ttcatcagtt cagctcccga tgctcagacc
      421 tgggctgata ggtatgttcg caactgtgca attgctattc ttcctctatt acctgaggga
      481 gttccagttg ctattttagc gggtatcacg acacgaatgc catttgcccc tatacatcca
      541 tcggcaatgt tgttaataat ggccacaggc cagactcaat agcttattac actattcaaa
      601 cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca gaatagcgtt
      661 ggtacacctg ctttatttct ggctatgatg aacggacaaa ctgacaacgt aaaaatattt
      721 atgcaagaaa ttcagtcact ggtatacaat catatcattc atgaagataa tctggttaaa
      781 ttactgcaaa ctaaaagtgc taacgaaaca cctggactct atatctccat gttgtatgga
      841 ttcgatgaaa taatcgatat ctttctgaat gcattaacca ctccaatagc acaagaactt
      901 ttaaacaaaa aaaatggtga tgagtatttt agccatgaaa atacatgatg gtgagccagg
      961 attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc tctctaaaat
     1021 taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt tattaaaagg
     1081 agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta atgaagacgt
     1141 cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt ttagtcaaca
     1201 tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg ttcatatagc
     1261 cattcatcat aagcattata aaactgtaga aacatattat gctgctataa atgtcatcag
     1321 ccaaagcctg agttttagtg ctgatgaaat aaagacg
//
LOCUS       DAZOYL010000490         1353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZOYL010000490 DAZOYL010000000
VERSION     DAZOYL010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.494"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            347..>1353
                     /gene="shiA"
                     /locus_tag="ACHUOY_004187"
     CDS             347..>1353
                     /gene="shiA"
                     /locus_tag="ACHUOY_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR0684770.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT          292 a          327 c          331 g          403 t
ORIGIN      
        1 gcaatacgtc tcaacaatga tgctaaaaac atacctaacc tcgcctccct actggttata
       61 atgcaatgca gtctatcaga ctcatcaggg tgccattttg tgcatatgcg gacttttatg
      121 tttcatatct ctaacctgtg ggtcctctgc ttaatcctta aacaacacca gcaactcctg
      181 cgctttcatc ttccatcgaa tttttcatgt tgccgctaat cagccataaa atcatttgca
      241 gatgcgctct gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt
      301 accttttcca tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct
      361 catctccact cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt
      421 aggcagcttc gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcatcaccgc
      481 cgcactggtg tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc
      541 cgcatttgct acctttggcg tcggattcct tttccgtccg ctcggcggtg tcattttcgg
      601 tcactttggc gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg
      661 catcgcgaca gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc
      721 tattttactg gtgacactgc gtgtcattca gggatttgcc gtcggcggcg aatggggagg
      781 cgcggcgttg ctttccgttg aaagtgcacc gaaaaataaa aaagcctttt acagtagcgg
      841 tgtacaagtt ggctacggtg taggtttact gctttcaacc ggactggttt cattgatcag
      901 tatgatgacg actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag
      961 catcgtactg gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt
     1021 tgaacaacag caacattatc aagctgccgc gaaaaaacgc atcccggtta tcgaagcgct
     1081 gttacgacat cccggtgctt tcctgaagat tattgcgcta cgactgtgcg aattgctgac
     1141 gatgtacatc gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggctaccgcg
     1201 cgaacttttc cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg
     1261 ttttgcctgg cttgccgatc gttttggtcg ccgtagggtt tatatcacag gtgcgttaat
     1321 cggaacgttg agcgcatttc ctttctttat ggc
//
LOCUS       DAZOYL010000491         1347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZOYL010000491 DAZOYL010000000
VERSION     DAZOYL010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.495"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            14..151
                     /locus_tag="ACHUOY_004188"
     CDS             14..151
                     /locus_tag="ACHUOY_004188"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR0684771.1"
                     /translation="MVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHH
                     I"
     gene            148..705
                     /locus_tag="ACHUOY_004189"
     CDS             148..705
                     /locus_tag="ACHUOY_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0684772.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(742..1005)
                     /locus_tag="ACHUOY_004190"
                     /pseudo
     CDS             complement(742..1005)
                     /locus_tag="ACHUOY_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(1117..>1347)
                     /locus_tag="ACHUOY_004191"
     CDS             complement(1117..>1347)
                     /locus_tag="ACHUOY_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0684773.1"
                     /translation="ASIQQEPNGWKTVTTQFYIQEFSEYIAPLQDAVDLEIATEEERS
                     LLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
BASE COUNT          364 a          350 c          287 g          346 t
ORIGIN      
        1 ccacattcgc attatggtgg ggcgtgcagg ggcggagaaa tccgcgccgg gttttgtgat
       61 gaccggttac gccaaccctg tacgtctcac cacctccctg attggcgtca gaggtggtga
      121 tcatccaatt aattggagtc atcacatatg aatacagcta taactttttc taacgagtca
      181 ccaaaagtct ttttcaaaga tggtcgcctg gttaccacct ctcaagctgt tgccgactac
      241 ttcgataaac aacataaaca cgttttagct aagattgaca cactcgactg ctctcccgaa
      301 tttacatcag ccaacttttc ggccgatgtt cagactgtcg aaattggcaa cggcgcagaa
      361 cgtgaatcac gatgctacct gatcacaaaa gacggcttca tgttcctcgt aatgggattt
      421 actggcaaaa aagcagccag actaaaagaa gcttacattg aaaaatttaa cgcaatggaa
      481 gaagaactcc acaaacgtcc tcctgcagca caaaactccc ccgccccaaa taatggatgc
      541 gcattactga tccacttcga taaacacggt caggtcgagt tcacagaaaa agtccccgcc
      601 gatgcgatgg tatgcactct ggaacggttt aaattttatc tggagcaaca tgggtggatc
      661 gttgcccgta aagaacaatt ggtggaaagg ttgatgcggt tttaatgaat gcaaaacccc
      721 ggatgatcac cgaggttaat tttatcgagc gtgattgccg ctaatcgcca tcgctctgat
      781 tgagcgtaaa tcattcaggt tattcagttc ttccttctgc tctcgctgac gtcaataaat
      841 cccatcattg cgatcaacct catccttaac cattgatgcg agcagttctt ccagtttctg
      901 cgctgacagc tttacctggt gatcttcagc atcttcccgg gctattgtgg ttcgcgcagt
      961 ggctgatttt gctgacatca ccacaggggg cagacggacc attgaaccat ggcctccgga
     1021 acaaataagg gcaaaaataa caaccactat cgaaagctca caaactaacc gcagcacgtt
     1081 cctgcatacg acgtgtctgc ggcataatcc caatgattac tccctgacag gatttgcagg
     1141 ccactcaata tcaggtgcag ttgatgtatc aacacgattc aacaataccc gatatttatt
     1201 ccatgcctcc agcaacgatc tttcttcctc cgttgcgatt tccagatcta cagcatcctg
     1261 cagtggcgca atatactcac taaattcctg gatgtagaac tgtgtggtga cggtcttcca
     1321 gccattcggc tcctgctgta tcgaagc
//
LOCUS       DAZOYL010000492         1347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZOYL010000492 DAZOYL010000000
VERSION     DAZOYL010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.496"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..185)
                     /locus_tag="ACHUOY_004192"
     CDS             complement(<1..185)
                     /locus_tag="ACHUOY_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR0684774.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(230..1216)
                     /locus_tag="ACHUOY_004193"
     CDS             complement(230..1216)
                     /locus_tag="ACHUOY_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0684775.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          371 a          298 c          273 g          405 t
ORIGIN      
        1 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
       61 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
      121 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
      181 atcattgtaa gcatcttgga aaatcagggc cagagactgg cccggaatat tattgagcag
      241 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
      301 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
      361 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
      421 cggatttctt cgctgctgtt tcgaggattt gtttctgttc tggggttaat ttattccatg
      481 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
      541 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
      601 ctacaccttg ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
      661 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
      721 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
      781 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
      841 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
      901 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
      961 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1021 tacggatcac catattacct tctgacagct gttttacctc tttcgccagc tcttcaaaac
     1081 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
     1141 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1201 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1261 tgtgcttatc agtgctttgt gcgacaaatt ggttaaccta tctgacaact tgtctatgtt
     1321 tatcacaact tgaaataaag tcagaaa
//
LOCUS       DAZOYL010000493         1341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZOYL010000493 DAZOYL010000000
VERSION     DAZOYL010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.497"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHUOY_004194"
     CDS             <1..184
                     /locus_tag="ACHUOY_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684776.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUOY_004195"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUOY_004195"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0684777.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZOYL010000494         1326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZOYL010000494 DAZOYL010000000
VERSION     DAZOYL010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.498"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            295..813
                     /locus_tag="ACHUOY_004196"
     CDS             295..813
                     /locus_tag="ACHUOY_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0684778.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1023..>1326
                     /locus_tag="ACHUOY_004197"
     CDS             1023..>1326
                     /locus_tag="ACHUOY_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR0684779.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          332 a          319 c          336 g          339 t
ORIGIN      
        1 aaaggataaa tacaatattt attcatgatt cagctttggc cattttatgg aggcaatacg
       61 gactttgaac agttgggtat ggttatccag atatatatta ttaagttatt gctgaataag
      121 agatttaatg attttaaaaa tagtttttac gacaaccgta aattctagct cggtttgtat
      181 gtaaaaaata ctgaaaaatg atgcggttcc gtgaagtgag ctgcatctgc ctgtctggga
      241 ttaacggcag gtcattgtaa gtagtaatta accacgttaa caaggagttt acaaatgcca
      301 acaccgtgtt atatctccat cactggtcag acccagggaa atattactgc aggtgcattt
      361 accgctgatt ctgtcggcaa catctatgtg caggggcacg aagacgagat gctggtgcag
      421 gaattcctgc ataacgttac tgtcccgacc actcctcagt ccggtcagcc tgcgggtcag
      481 cgtgctcaca agcctttcat ttttaccgtg gcgctgaaca aagcggttcc gctgatgtac
      541 aacgcactgg cctccggcga aatgctgcca accactgaac ttcactggtg gcgtacctct
      601 gtggagggca aacaggagca ttatttcact acccgcctga ctgattcgac gattgtcgat
      661 atgaaactgc atatgccgca ttgccaggac ccggcgaaac gcgagttcac ccagttgctc
      721 gaggtttcac tggcctaccg caaaattgaa tgggaacacg tcaaatctgg cacctccggt
      781 gccgacgact ggcgcgcacc actggaagca taagttaagc caacagcatc cggctatcag
      841 gccggatgct gtttttgtat tacgccgtag aaagaaaggt tgtcagtaag gggttacgtt
      901 cacgggagat ggcggatgcc ggaaacttcg gcggtagcgt gcctgaacgc aagcgtaaca
      961 ctgtactgtc acagccgctt taacagcgaa ctgtctgttt ttccttaacc ccggaggttg
     1021 ctatgtcaac cggattacgt ttcacgctgg aagtggacgg cctgccaccg gatgcttttg
     1081 cggtggtctc ctttcatctg aaccagtcac tctcttcgct cttttccctc gagctctctc
     1141 tggtcagcca gcagtttctc tcccttgaat ttccgcaggt actggacaaa atggcctacc
     1201 tgacgatatg gcagggcgat gacgtacagc gccgggtgaa aggtgtggtg acctggtttg
     1261 aactggggga gaacgacaaa aaccagatgc tgtacagcat gaaggtatgc ccaccgctgt
     1321 ggcgca
//
LOCUS       DAZOYL010000495         1316 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZOYL010000495 DAZOYL010000000
VERSION     DAZOYL010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.499"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            63..1226
                     /locus_tag="ACHUOY_004198"
     CDS             63..1226
                     /locus_tag="ACHUOY_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0684780.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          318 a          310 c          364 g          324 t
ORIGIN      
        1 tcaatgatcg gaagtatgcc aaatcgtgat cgattgtgat gccaaactgt gatcggagat
       61 ggatgccaaa tcatgatcga ttcgaatgcc aaactgcgat cgattcagat gccattttgc
      121 agctggcgtg ggcggtcttt aaaatctttc gtattccggt gaatcagtgg acgctggcga
      181 cggcggcgct gggaggcgtg tttctggtga gtggtttgat tttgttgatg aactacaacc
      241 acccttacac ttttaccgcg caaaaggcag tgatagcgat ccctatcacg ccacaggtga
      301 cgggaattgt tactgaagtc actgacaaga ataatcagct tattcaaaag ggcgaggtgc
      361 tttttaagct cgacccggtt cgttaccagg cgcgagttga cagacttcag gctgacctga
      421 tgacggcgac gcataatata aagacgctgc gtgcgcagct cactgaagcg caggccaaca
      481 ccacccaggt ttcagcggag cgcgaccgtc tgtttaaaaa ttatcaacgt tacctgaaag
      541 gcagccaggc ggcggtgaat ccgttctcgg aacgtgacat cgacgatgcg cggcaaaatt
      601 tcctcgcgca ggatgcgctg gtgaaaggct cggtggcgga gcaggcgcag atccagagcc
      661 agctcgacag tatggttaac ggcgagcaat cgcagattgt gagcttaaga gcgcaactta
      721 ctgaagcaaa atataacctt gagcagactg tcattcgcgc gccgagcaat ggctacgtta
      781 ctcaggtact gatccgccca ggtacatacg cagctgcctt gccgctgcgt ccggtgatgg
      841 tcttcatccc cgagcaaaaa cggcaaattg tcgcccaatt tcggcaaaac tcgctgttac
      901 gtctgaaacc tggcgatgat gcggaagtgg tgtttaacgc gctacctggg caggtgttcc
      961 acggcaaatt gactagtatt ttacctgtcg tgccaggcgg ttcttatcag gcgcaggggg
     1021 tattgcaatc attaacggtc gttcccggca cggacggcgt gctgggaacc attgaactgg
     1081 accctaacga tgatatcgat gccttacccg acggcattta cgcccaggta gcagtttact
     1141 ccgaccattt cagccatgtt tcggtgatgc ggaaggtgct gctaagaatg accagttgga
     1201 tgcattattt gtatttggat cattgagggg gaagggggag attatggaac tttgctccct
     1261 gcaagggcta attatttttg catattactt cattggctgc gataattttt ctttaa
//
LOCUS       DAZOYL010000496         1310 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZOYL010000496 DAZOYL010000000
VERSION     DAZOYL010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.500"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            45..>1310
                     /locus_tag="ACHUOY_004199"
     CDS             45..>1310
                     /locus_tag="ACHUOY_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR0684781.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKR"
BASE COUNT          434 a          248 c          289 g          339 t
ORIGIN      
        1 aagtggttat ttacgctttt aacaaacgat tgatggaata ttttatgaaa gggaaatctg
       61 cattaactct tctgctcgct ggtatatttt cgtgcggcac atgccaggcg acaggggcag
      121 aagtgacgag tgaatctgta tttaacattc ttaattccac gggggcagca acagataaaa
      181 gttatttatc gttaaatcca gataagtacc ctaattatcg tcttctgatt cactcagcca
      241 aattaaaaaa tgaaataaag agccactata ccaaagacga aattcaaggg ttgttaacat
      301 taacagaaaa cacacgaaag ttaacgttga cggagaaacc ctggggaaca tttattcttg
      361 cttctacttt cgaagatgac aaaacagcgg cagaaaccca ctatgatgct gtctggctga
      421 gggatagttt atggggctat atggcattag tatcagatca gggaaatagt gtagccgcaa
      481 aaaaggttct gctcaccctg tgggattata tgtctacgct cgaccagatt aagcggatgc
      541 aggacgtcat tagcaacccc aaacgactgg atggagttcc cgggcaaatg aatgctgtgc
      601 atatccgatt tgacagcaac tcccccgtga tggcagatgt gcaggaagaa ggcaagccgc
      661 agctgtggaa ccataaacaa aatgatgcgt taggtcttta tcttgatctc cttatacaag
      721 caatcgacac tggtacaatt aacgctgaag attggcaaaa aggtgaccga ttaaaatcgg
      781 ttgcgctgct gattgcttat ctggataaag ccaatttcta tgttatggaa gattccggag
      841 catgggaaga agatgcccga ttaaacacat cttccgttgc actggtgacc tctggactgg
      901 aacgtttgtc gaacttactc tcgaagaaag actctgtttt tgtctctgat ttactacgcg
      961 aagctaaagc caatgaactg gatgaacctc tttcaacaac gaggttgaac cacctcatag
     1021 ataaaggtta tgaaagaata acgttacagc ttgatttagg gggagaatcc ccaggttatc
     1081 tggaaaaaga taaacattac agagaagccg atgcggcttt acttaatgtt atttatcctg
     1141 caaatcttgc aaagataaac accagaagaa aagagcaggt tctaaaaata gttaaaaaac
     1201 tagcaggtcc gtatggtata aaaagatacg agaaggataa ttatcagtcg gcaaattttt
     1261 ggttcaatga tattaaaacc gataccgatc aaaattcaca tgcgaaaaga
//
LOCUS       DAZOYL010000497         1302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZOYL010000497 DAZOYL010000000
VERSION     DAZOYL010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.501"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            195..872
                     /locus_tag="ACHUOY_004200"
     CDS             195..872
                     /locus_tag="ACHUOY_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR0684782.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            944..1228
                     /gene="ppnP"
                     /locus_tag="ACHUOY_004201"
     CDS             944..1228
                     /gene="ppnP"
                     /locus_tag="ACHUOY_004201"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR0684783.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          336 a          282 c          317 g          367 t
ORIGIN      
        1 ataattcatg tttttctaat aattagaata ttaaacaata acaatccatt actggaatca
       61 tttggaatct ttacattatg ccgtgcacgt ctgctgctac gctttttgtc atttgtagca
      121 caagtaagtg tcagcagtgg tgctccacac ttgcccggta attaacgacg aaagaaaagt
      181 aaggtggatg aacaatgagt gcgtcgttgg cgatcctgac catcggcatt gtacctatgc
      241 aggaggtttt gccgctcctg acggaataca ttgacgaaga taatatttcc catcatagcc
      301 tgctggggaa gttaagtcgt gaagaagtga tggcggagta cgcaccagaa gcaggcgaag
      361 acaccattct cacattatta aatgacaacc agctggccca tgtttcgcgt cgtaaagtgg
      421 agcgagacct gcaaggtgtg gttgaagtgc tcgataatca gggttatgac gtcattttat
      481 taatgagtac agcaaacatt agtagtatga ctgcgcgtaa tacgatcttt cttgagccgt
      541 cgcgaatatt gcctccactg gtttcctcta ttgttgaaga tcatcaggtg ggggttatcg
      601 ttccggttga ggagatgctg accgttcagg cgcaaaaatg gcaaattttg cagaaatcgc
      661 cggtattttc attgggtaac cccattcatg attcagaaca aaaaatcatt gatgccggga
      721 aagaattact ggcaaaaggg gctgatgtca tcatgctgga ttgtttagga tttcaccaac
      781 gtcatcgcga tttactgcaa aaacagctcg atgttcctgt cttgctgtct aacgtattga
      841 ttgcacggct ggctgcggaa ttactggtgt aattttgcgt gacagccagc gcctctggcc
      901 cctatagtga agtagatgtt caactaccag acagggccag tttatgcttc aaagtaatga
      961 gtacttttcc ggcaaagtga aatcaatcgg cttttccagc agcagcactg gtcgcgccag
     1021 cgtgggtgtt atggttgaag gcgaatacac cttcagcacc gctgagccgg aagagatgac
     1081 ggtaatcagt ggcgcgctga atgtgttact gcctgacgcg accgactggc aggtgtatga
     1141 agccggttcg gtgtttaatg ttcccggtca cagtgagttt catctgcaag ttgccgaacc
     1201 cacctcttat ctgtgccgct atctgtaatt cctcgccttc cccttgaacg ggagggcatt
     1261 tttctgaaat atcctctctt taacccataa taatatttcc tt
//
LOCUS       DAZOYL010000498         1302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZOYL010000498 DAZOYL010000000
VERSION     DAZOYL010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.502"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..882)
                     /gene="tdcA"
                     /locus_tag="ACHUOY_004202"
     CDS             complement(<1..882)
                     /gene="tdcA"
                     /locus_tag="ACHUOY_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR0684784.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELA"
     gene            1197..>1302
                     /locus_tag="ACHUOY_004203"
     CDS             1197..>1302
                     /locus_tag="ACHUOY_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR0684785.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLF"
BASE COUNT          419 a          276 c          236 g          371 t
ORIGIN      
        1 ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg accataccgc
       61 ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt gattagaacc
      121 aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga gaacaagatt
      181 ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc catttctttg
      241 taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca cccactgttc
      301 gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac tggctaccag
      361 cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca tttctgcact
      421 taatgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga acgaagacag
      481 ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact tgttgatcat
      541 ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt ccaccaccgc
      601 ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg taatggattc
      661 ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat ttttccgcac
      721 cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga cggccggttg
      781 agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga cttcctgaaa
      841 gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct acgttaatta
      901 cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc gaccactcac
      961 aaattaactt tcaatacttt ccagggtatc gtcattacaa ttaaatacct ttaaatatca
     1021 acaagttaaa gcataaaaat cagcataaac cctgattttt gtcaaaaaaa atgacgggga
     1081 taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa aaggggctga
     1141 gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta aatttaatga
     1201 gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact gcggcacgtc
     1261 acattttttc acgctatttg ttggagaaca aacatttatt tt
//
LOCUS       DAZOYL010000499         1294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZOYL010000499 DAZOYL010000000
VERSION     DAZOYL010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.503"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..1112
                     /locus_tag="ACHUOY_004204"
                     /pseudo
     CDS             1..1112
                     /locus_tag="ACHUOY_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1090..>1294
                     /locus_tag="ACHUOY_004205"
     CDS             1090..>1294
                     /locus_tag="ACHUOY_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684786.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAZOYL010000500         1292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZOYL010000500 DAZOYL010000000
VERSION     DAZOYL010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.504"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..927
                     /locus_tag="ACHUOY_004206"
     CDS             <1..927
                     /locus_tag="ACHUOY_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR0684787.1"
                     /translation="AIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTM
                     TMQDHATLKEIKDLAKNITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGIT
                     MNCYSPLCKNASRMDQKLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEM
                     RSLGSYIAFNLEGSSQERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDC
                     YNNVYRLSLPPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAM
                     ADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT          415 a          246 c          275 g          356 t
ORIGIN      
        1 gccatctttc ctgttattcg ggcgcttgaa ttgcataaaa ttaagcatct tttaattgta
       61 acgtcgacaa ttaatttaga agtctttaat gcgcttaaac ttgaacagac taaattaaca
      121 ttagtcacaa tgactatgca ggatcacgca acattaaaag aaataaaaga tttagcaaag
      181 aacataacac agcaatacgg tacgccggat ctttgtattg aggggatgcg taaaaagaac
      241 ctgaaaacga tgttatttat cagtcagttg aaagcaaaaa cgaattttca ggttgttggt
      301 ataaccatga attgctactc ccctttgtgc aagaacgcgt cccgtatgga tcagaaactc
      361 cgggctcccg tacctatgac atgggcattt atgatgcgtg aggcgggttt tccagcagtc
      421 aggccaatat atgaattgcc actaagtgag gatgtactcg atgaggtgcg cgaggaaatg
      481 cgatcgttag gatcttacat tgcgttcaat ttagaaggta gctcgcagga acgtacattt
      541 tcattatcga ttgcagaaaa tctaatagca aaaattcaaa gtgaaacaga tataccaata
      601 gtgatcgttc atggacccaa aggtgaagat aaagccaggg cattagtgga ttgttataat
      661 aatgtctacc gtttatcctt accaccctcg attaaacgtt cagcagcaat cataaaagat
      721 gcttatatcg caataactcc tgacacctca atattacata tggcaagtgc ctataatacc
      781 cctgttgttg caatttatgc tgattacaaa acgcgatggc ccgcaatggc cgatgtctcg
      841 gagtcagtcg tcgttgggca aaaaattgac aatataagtc tggatgaatt cgcaaaggca
      901 ttaaaaagtg ttttggcgag aatatgagac agtacttatg catagtaatg ccgatctgtt
      961 atgaacggtg gtgtcatcaa tactctgagg ctggtcaatc atcacagaaa accagccttt
     1021 gaatcaaagc agtactcacc ggatacgatc atagtcacat cgaacgcaat tctggcggtg
     1081 ccgcattttt atttattcat tttctgtgag agaagggcaa gctctataga gggaatgtaa
     1141 aaaagagagc tccgaatgga gcactcttta agaaattata acggaaaatc agaggttttt
     1201 ataacatgtc gcataagcaa acccaagctt ttcaaatacg ctttgatcac ctgttgccag
     1261 ggaaactacg tattgttcgc ttgagtttgc gg
//
LOCUS       DAZOYL010000501         1282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZOYL010000501 DAZOYL010000000
VERSION     DAZOYL010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.505"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            132..1151
                     /locus_tag="ACHUOY_004207"
     CDS             132..1151
                     /locus_tag="ACHUOY_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR0684788.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          368 a          285 c          357 g          272 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aa
//
LOCUS       DAZOYL010000502         1263 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZOYL010000502 DAZOYL010000000
VERSION     DAZOYL010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.506"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..689
                     /locus_tag="ACHUOY_004208"
     CDS             <1..689
                     /locus_tag="ACHUOY_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0684789.1"
                     /translation="SQKDVFMTYRTTGLATYRNYDFFGLIEGLNFAAQYQGKNERTDN
                     SHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAE
                     LWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAY
                     LKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDN
                     IVATGITYQF"
     gene            853..>1263
                     /locus_tag="ACHUOY_004209"
     CDS             853..>1263
                     /locus_tag="ACHUOY_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684790.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKESKLENIYIDHNN"
BASE COUNT          390 a          269 c          243 g          361 t
ORIGIN      
        1 ggagtcagaa agatgtcttc atgacatacc gtaccaccgg tctagcaacc tatcgcaact
       61 acgatttctt tggcttaatt gaagggctga actttgccgc gcaatatcaa ggcaaaaatg
      121 aacgtactga caacagtcat ctttatggtg ctgactacac gcgtgccaac ggtgacggtt
      181 tcggtatctc ctcaacttat gtttatgatg gctttggtat cggtgcggta tataccaaat
      241 ccgatcggac aaatgcgcag gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga
      301 atgcagaact atgggccaca ggtataaagt atgatgccaa caacatctac tttgcagcta
      361 attacgctga aacattaaac atgaccacct atggggatgg ttatatctct aacaaagcac
      421 aaagttttga agtggtggcg caatatcaat tcgacttcgg cttgcgccca tcactcgctt
      481 acctgaaatc gaaaggcaga gatctgggcc gctacggcga ccaggacatg attgagtata
      541 tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt gattataaaa
      601 tcaacctgat tgatgaaagc gactttaccc gtgccgtaga tattcggacc gataacatcg
      661 tcgctacggg cattacctat cagttctaac gtcctctcct gaccagccct tctctgggct
      721 ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa tttttgatat
      781 ttctggtcat tcacctcaat taatattatt ccatatcctc cggggacatc ttactataat
      841 atttattgat gagtgattat gaccaatatt aatacagctt gtgtaaaaaa taatgccagt
      901 tatcaactta ataacgcatt acctaacaaa gaaaccatct ccagcaattt ttgtgaacga
      961 ctagaacaat ggggtaataa atcggttaat aatggtgaag aaagagcaat tgccgtggag
     1021 cgaattaaag aagcttacaa ttcgaacatg gcatctcttg atttgtcata tcttgatttg
     1081 agtgaattac cgcctatccc ctcgacagtt aatacgttaa atctggaaaa caattgtcta
     1141 acttgtcttg acttagctga caatgccagt ctcgttaata tcaacctcag cgttaataaa
     1201 attaacacga taacctttcc aaaagaatca aaactggaaa acatttatat tgatcataac
     1261 aat
//
LOCUS       DAZOYL010000503         1262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZOYL010000503 DAZOYL010000000
VERSION     DAZOYL010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.507"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            62..563
                     /gene="tnpA"
                     /locus_tag="ACHUOY_004210"
                     /pseudo
     CDS             62..563
                     /gene="tnpA"
                     /locus_tag="ACHUOY_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(581..1249)
                     /locus_tag="ACHUOY_004211"
                     /pseudo
     CDS             complement(581..1249)
                     /locus_tag="ACHUOY_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          331 a          289 c          282 g          360 t
ORIGIN      
        1 ctcctgttga ccacctggtc ttaactggaa tttaaatgcc tgtaatcgtt tcacaggtca
       61 aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
      121 cctgatgcat gttcacctgg tctttgtcac tagataccga cgccagattt tttgacgcga
      181 cagaaaaact acgcacttac ttttcaaatg tatgtgctta ttttgaagct gaactggttg
      241 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctaacctccc aaactggcga
      301 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      361 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      421 gtagctgcgg aggtgtgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      481 ctggtcaggt ggaaaaccgc gccttatatc cccgccctga aggatggggt tttacggcgc
      541 accggataaa aagcgccatc taacgatggc gcgtctggaa ctactttttg ttaacgtcaa
      601 acatggtggc atcggtcgcc atgtcatcaa taacttgttt aatattttcg aaagccattg
      661 gcgtactttc gttattcagg tctttacctt cgccctgacg aacgactttg ataaccggtt
      721 tattagtcgc tgcatcaatc agctcacctt caaaatagag tcgagtatcc atggtacggt
      781 ggcctgtagc catttgcgtc cccgcaacca ctaatgcaac aggaaccact tcatagaatt
      841 gcagcccttc tttgctggta tctacaccgg taatggcccc acggaaaatc agactacgcg
      901 gcccagcggt ggtaaccagt ggtttacgct gcgctatcgc ttctttcatt tcggtgttgg
      961 tatagttcaa aattttatcc agaactttct gccctaccta ggtagaaggt tttggcaccg
     1021 gataataagt gattgggttc cagacgatgc tgtcatattt gctttgatca aaactcgggt
     1081 ctacccaacg taaaacaggt ttacctgttg ctgaggttgt ttcttttaaa tcagagtaat
     1141 tgtttaaaaa accagaatat ttatctggct gggtgatttt agaggcacaa ccagacagag
     1201 ccaacaagcc gcataaagct gcaactttcg caaatgatgt ggtacgcatg aatagtttcc
     1261 tg
//
LOCUS       DAZOYL010000504         1262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZOYL010000504 DAZOYL010000000
VERSION     DAZOYL010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.508"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..488
                     /locus_tag="ACHUOY_004212"
     CDS             <1..488
                     /locus_tag="ACHUOY_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0684791.1"
                     /translation="TETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIYAY
                     AFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYNYS
                     PYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSEDN
                     G"
     gene            823..>1262
                     /gene="ygeH"
                     /locus_tag="ACHUOY_004213"
     CDS             823..>1262
                     /gene="ygeH"
                     /locus_tag="ACHUOY_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFR0684792.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIIS"
BASE COUNT          457 a          197 c          208 g          400 t
ORIGIN      
        1 gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg
       61 cattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata
      121 gaatatatgc atatgcattc gactaccatg aaaagggtaa tataacggac gcagaaattt
      181 attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa gattttgcat
      241 cagtatgtca gccaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca
      301 attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg
      361 ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg
      421 atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata
      481 atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt taactactat
      541 aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata
      601 aatcattatt tttcaatgcg ttagagaaca atctacttta taaaaattga ataaggctat
      661 ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata gatatgtcga
      721 atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt tctattgctt
      781 aagcaggatg caagaaacca attttttcat agaggttaac taatggactt agaaaataaa
      841 ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct tactcaagga
      901 aatgagcaag tttatattcc acagaaagag ttaggtgtat taatagtatt acttgaatcc
      961 gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa tattattgtt
     1021 agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga aaaaattggc
     1081 tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagcgg gcaggttttc
     1141 aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt ccctttcact
     1201 acttcattga atacactgga tccattaata cttaatcagg aattagtgca aatcatttca
     1261 aa
//
LOCUS       DAZOYL010000505         1260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZOYL010000505 DAZOYL010000000
VERSION     DAZOYL010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.509"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(19..>1260)
                     /locus_tag="ACHUOY_004214"
     CDS             complement(19..>1260)
                     /locus_tag="ACHUOY_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0684793.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYPTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          332 a          297 c          227 g          404 t
ORIGIN      
        1 gaaccctaac aaaaaatact aagcgtccac ctcttccata tcaccagcag gataaatcct
       61 tgttactggt tcaccaaaaa taagcatatc atctaatgac tgcatctcat cggttgcttc
      121 ttcaagcgtt aaggtgtggt catctttacc atcaagagga aaaccacgaa tcatatttcc
      181 attgtgaaga tatatatcca ccagcttttc attaccagaa acaacaatct ctttcaccag
      241 acgcttaata ataatctggg cttcagttct cccctctaca gactccatat caagaccgct
      301 taaatccaag cttgatacag attttaccct cgccttttct cgtgataact gaactatcag
      361 ttcatctttt tcttcctgtt taacccgtag tgcttcttga atctgagtga tgttaggaag
      421 ctgtatcagc gtctcagtga gtttttccag ttcaccatca acggtgttta atcttctttg
      481 tagttcatct aatttagctg tatcggtagc ttcatcgtgt agctgtgaca gtttgctaaa
      541 cagacgacta caaagctgtg tatcaagtaa ctttctggac actgtaccgt agctacaacg
      601 ggactcctta ttaccattgc aacggtaagt accttgataa acgggtttac gtatccctgt
      661 aggagtcatt acaagcccac acttacaacg gataagcccc ttcagtatgt tcacatatag
      721 ccagtgttca ccgctggatg ttttcccctt ccctgtttcc tctaacagtt gctgtacagc
      781 gtaaaactta gagtctgtga caatctgagg atagaaccct tcaatttctt cacgtaacac
      841 tgttttacgg tcttctgtag aaatcttagc gggaaggtaa cctaatacac ttcgtgaagt
      901 caggatgtgt ttaacgctta atgctgacca gttgccatcc ggtttcttag tcttggatat
      961 tctgacctta cgctgattaa tggtcgggta accttgttcg tttagccatt tacagatagc
     1021 aaacatcgac ataccagaaa gacgcttgtc atagatgata ttcaccagtg acgctttatc
     1081 ttcatcaacg atgtagtatg aaccatcttc tgatagctct aaccatccgg gggcttgctt
     1141 acccataggc ttcttaagtt ctcttgcaag ctgtgtttta tggttccaga cctgagctat
     1201 acgcttttgc ttaacaagag attcttggtg tgctctctca gctatgatga tgtgcttaat
//
LOCUS       DAZOYL010000506         1258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZOYL010000506 DAZOYL010000000
VERSION     DAZOYL010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.510"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            3..1198
                     /gene="bluF"
                     /locus_tag="ACHUOY_004215"
                     /pseudo
     CDS             3..1198
                     /gene="bluF"
                     /locus_tag="ACHUOY_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
BASE COUNT          361 a          265 c          275 g          357 t
ORIGIN      
        1 taatgcttac cacccttatt tatcgtagcc atatacgtga cgacgaacct gtcaaaaaaa
       61 tcgaagaaat ggtttcgata gcaaatcgca ggaacatgca gtctgacgta acagggatct
      121 tactgtttaa tggttctcat tttttccagc ttctggaagg tccggaagaa caggttaaaa
      181 tgatatatcg ggctatatgc caggatccac ggcactataa tattgttgag ctgctgtgcg
      241 attacgcgcc tgctcgccgt tttggcaaag cgggaatgga attatttgat ttgcgcctgc
      301 acgagcgaga tgacgtttta caggccgtat tcgacaaagg cacatcaaaa tttcagctaa
      361 cttatgatga cagagcgcta caattttttc gtacttttgt ccttgcaacc gaacaatcaa
      421 cctatttcga gatccctgcc gaagactcct ggctttttat cgctgacgga tctgataaag
      481 aacttgattc ctgtaccctt tcaccaacta taaacgacca ctttgccttt catcccttat
      541 cgcggcggat aatcgctttt gaagccattg tgcaaaaaaa tgaagcccat cagccatagc
      601 ggttgggcag cgtaaagacg gggaaatcta cacagcggat ctcaaaagta aggcgcttgc
      661 attcgcgatg gcacacgcac ttgagctcgg tgataaaatg atttcaatca atctattacc
      721 tatgaccctg gttaacgaac ctgacgcagt ctctttttta cttgatgaaa taaaggccaa
      781 tgctctggtg cctgaacaaa tcatcgttga atttactgaa agtgaagtta tatctcggtt
      841 tgatgagttt gcagaagcga ttaaatcgct taaggctgtc ggtatcagtg tagcaattga
      901 tcattttggc gcaggttttg ccggtttgtt actcctgtca cgcttccagc ctgacagaat
      961 taaaatcagt caggaattga ttaccaatgt tcataaaagc gggccacggc aggcaattat
     1021 tcaagcgatc ataaaatgct gtacatcact tgaaattcaa gtcagtgcta tgggcgtggc
     1081 aacaccagaa gagtggatgt ggcttgaatc cgcaggaatt gagatgtttc agggagatct
     1141 gtttgctaaa gctaaattga atggtatccc ttccatagcg tggccagaga aaaaataatt
     1201 ttcagcacat tctttcacat gatttcagta aatcatcgtg aaaatgaata cgttatgt
//
LOCUS       DAZOYL010000507         1249 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZOYL010000507 DAZOYL010000000
VERSION     DAZOYL010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.511"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..153
                     /locus_tag="ACHUOY_004216"
     CDS             <1..153
                     /locus_tag="ACHUOY_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR0684794.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            257..1171
                     /locus_tag="ACHUOY_004217"
     CDS             257..1171
                     /locus_tag="ACHUOY_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0684795.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAADGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          363 a          256 c          285 g          345 t
ORIGIN      
        1 actgccgaaa atattcagat agagctgagg gatgaccagg atgctgcgtt aaaaaatggc
       61 gatagcaaaa cggttattgt tgatgagatc actcgtaatg cacagtttcc acttaaggca
      121 agagctatca cggtgaatgg aaacgcaagc tagggaacga tcgaggcgct aatcaatgtg
      181 atctacacct ggcaataacg tcgattcgat tttcttagcc ttctttttaa atcaattttt
      241 aaccttagga tatatcatgg gtaaaacaat cagtataaag gtcctgttcg gtatttatct
      301 tctattaatg gcaggcaaag tttttgcctt cagttgtaat gtcgatggcg ggtcaagtat
      361 tggagcaggt acaacctctg tttatgttaa tctcgaccct gtaatacagc cgggccagaa
      421 tctggttgta gacttgtctc agcatataag ttgctggaat gattacggcg gctggtacga
      481 cactgatcat ataaacctgg tacaaggctc agctttcgca ggctccctac agtcatataa
      541 aggatcgctg tactggaata atgtgaccta ccccttcccg ttgacgacaa ataccaacgt
      601 attggatatt ggtgataaga caccgatgcc attaccgtta aaactctata ttaccccagt
      661 tggggcggcc gatggagttg tcataaaagc gggggaagtc attgcccgta ttcatatgta
      721 taaaattgca acattaggaa gcggaaatcc gcgtaatttt acgtggaata ttatttcaaa
      781 taacagtgtt gttatgccaa caggcggctg caccgttgat agtcgtaatg tcactgtgga
      841 tctcccggac tttcctggaa gcgcagaaat accccttggc gtatattgct caagcgaaca
      901 aaagctctca ttttatcttt cgggtgccac cactgacagt agccgacaag tgtttgctaa
      961 cacagcacct gatgcaacaa aagcatcggg agtgggtgtc actctaatgc gtaatggaaa
     1021 aatactggct acgggagaaa atgtctccct ggggacagta aataaaagca aagtacctct
     1081 gggtttaagc gcaacctatg gacaaacagg aaacaaggtt tcagccggaa ccgtccagtc
     1141 agtcattggt gtcactttta tctatgaata attgtgattt tgaccttagt ctatgcgctc
     1201 cattcacgaa aattgcgcag ataattattt tgtgaaggct attatccta
//
LOCUS       DAZOYL010000508         1244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZOYL010000508 DAZOYL010000000
VERSION     DAZOYL010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.512"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            29..1156
                     /gene="ltrA"
                     /locus_tag="ACHUOY_004218"
     CDS             29..1156
                     /gene="ltrA"
                     /locus_tag="ACHUOY_004218"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR0684796.1"
                     /translation="MKDRNGSGAKGLPHCADGAAATTGDNADGRTAVKSAKPFPVSKR
                     QVWEAYKRVKANRGAAGIDGQTLAGFDENVTDNLYKLWNRMASGSYMPQAVRRVDIPK
                     ADGGVRPLGIPAVSDRIAQMVVKQILEPVLEPLFHADSYGYRPGKSAHQAIAQARKRC
                     WKFDWVVEVDIKGFFDDIDHDLLLKTVQHHTQARWVVMYIERRLKAPVQMPDGAMLAR
                     GRGTPQGGVISPLLSNLFLHYAFDMWMQRQFPGVPFERYADDVVCHCHSQWQADALIS
                     GLRQRLAQCGLQLHPQKTRIVYCKDADRRGDYPETSFDFLGYTFRPRLSMNRWGKTFV
                     NFSPAMSARAGKAIRQEVRRIAVTSPCTSWRICSMRKSEAG"
     gene            1126..>1244
                     /locus_tag="ACHUOY_004219"
     CDS             1126..>1244
                     /locus_tag="ACHUOY_004219"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HFR0684797.1"
                     /translation="MFNAKIRGWIKYYGAFYKSALYLTLRQIDRKLVLWLPRK"
BASE COUNT          305 a          295 c          379 g          265 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc tttgatgata tcgatcatga cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tgtcacagcc agtggcaggc
      841 tgacgctctg ataagtggac tgaggcagcg gcttgcgcaa tgcgggctgc agctgcatcc
      901 gcaaaaaaca cgaatagttt actgtaagga tgctgaccga cggggggact acccggaaac
      961 gagcttcgac tttctgggct atacattccg gccgcgtctg tcgatgaatc gctggggtaa
     1021 gaccttcgtt aacttcagtc cggcgatgag tgccagagca ggaaaagcaa tccgtcagga
     1081 ggtgaggaga atcgcagtga caagtccttg tacgagctgg cgcatatgtt caatgcgaaa
     1141 atcagaggct ggataaagta ctacggtgct ttttataaat cagcactgta tctgacgctg
     1201 aggcaaatcg atcgcaaact cgtgttatgg ctgccccgta aaca
//
LOCUS       DAZOYL010000509         1241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZOYL010000509 DAZOYL010000000
VERSION     DAZOYL010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.513"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            117..800
                     /gene="tssD"
                     /locus_tag="ACHUOY_004220"
                     /pseudo
     CDS             117..800
                     /gene="tssD"
                     /locus_tag="ACHUOY_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            780..>1241
                     /locus_tag="ACHUOY_004221"
     CDS             780..>1241
                     /locus_tag="ACHUOY_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684798.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT          386 a          243 c          266 g          346 t
ORIGIN      
        1 agtgaaacat tttgtttttc acattttagt tcatcacttt taggcgtaaa taaaggtaaa
       61 aaaaacaaca atactatttc tctttcacaa aagaattaac aaaaggtttt tcaaggatga
      121 gtgatattat ttacctgacc ataaccggag aacaacaagg atgtatttcc tctcgttgtg
      181 gaacgagcgc atcaataggt aatcgctggc agattggcca tgatgatgaa atcttcgcgt
      241 tctcgctctc taatagtatt acgaatacag gcaaagggtc ataattgcat ggccttagtt
      301 tctgtaaatt gattgacaaa agctccccgt tacttattaa cgctattaac aataacgagc
      361 agttgttcat ggagtttgac ttctatcgga tcaataggtt tggtcgatgg gagaaatatt
      421 acaacattca actccgcggc gcgctcttat cagcaatcaa tcacctgttt actgagaaca
      481 atctggatac agaagccatc actgtcagtt attaatatat actctccagg catctgattg
      541 ctaatactga atttagttat tgggcatttc ccgataacta caaccgtctg tttataccac
      601 ggccaaaaac taaagctggt aatggtttca aaacacttaa tagtaaagcg gttggcagac
      661 tgcttgctgc tgtcggcata tacaatggga atatcgaagg attcagggaa acagcagaca
      721 agttgggggg agatgcacct gctggttatg atcaatttat ggataacaaa gggttactca
      781 tcgctggtgc ttcaatagtt gctggagcag ttctggggag aatgagactc cctgatttgg
      841 aatcacttga gcatttcgga gccagaggag cagtatcagg aagaccattt aatcccgagc
      901 ttgccggtgg tccaatagaa aacctgacga cagatggcgt aacaatcaac agggaaggta
      961 tcgctattgt tgaaaaacat atagccagat ttggtcatga tcctgtaaat gaagtgatga
     1021 tcaatagact gaaagacatt gagaaaggaa aaatcccccc agaacaagta gatcttaact
     1081 tctatactca tgagtgtcgg gaatatcagc gttactgtaa tcttggttgg gaaacaggac
     1141 aacctgatgg tgacgcaggc tatgcactct ggaatcatac tggatttgcc cctatatttc
     1201 cagacatctg ttatcactta acccattaca agcccgctgc c
//
LOCUS       DAZOYL010000510         1238 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZOYL010000510 DAZOYL010000000
VERSION     DAZOYL010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.514"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..153
                     /locus_tag="ACHUOY_004222"
     CDS             <1..153
                     /locus_tag="ACHUOY_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0684799.1"
                     /translation="AAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVL
                     AGSGLS"
     gene            164..601
                     /locus_tag="ACHUOY_004223"
                     /pseudo
     CDS             164..601
                     /locus_tag="ACHUOY_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            601..978
                     /locus_tag="ACHUOY_004224"
     CDS             601..978
                     /locus_tag="ACHUOY_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684800.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          403 a          177 c          318 g          340 t
ORIGIN      
        1 gccgcagaat tattttcagg gatacggcac atcgctatta atattttaac gaatgataag
       61 gtattcaagg cagggttaag acgtaagatg cgaaaagcag ccatggatag aaactatctc
      121 gcgtcagtcc ttgcggggag cgggctttcg taatctttcc ctagggagtc agccgaatgt
      181 tgggaaggat ttgacggatg aagagaagaa ctgcttggcc ggcggcagta cgggaagccc
      241 gggaggatgg ggaccagaag atgagaatag cgcaagggat cgtatgcagc atgaacggta
      301 taaagatgaa ttgcgacaga aaatgggaaa acccgacgtt acagattcaa atttgaaaaa
      361 tattatagac gatctttatc ggccaaatgc aaaagtagga agtggaagca cagcagatgc
      421 tgttcgttat gaattggcca caggtgaaaa agttggtggt agaggacatg tattgaaagc
      481 acaggattat acaagggctt tacaggattg gttggataag aatccaacag catctccagg
      541 ggatcgagca gcagcagaaa acgttcttca ggatttacag aatgctttgg gaggcaagta
      601 atgaaccaat atattgctag ttatatagaa aaaatggcat cattttccac tgttttgtct
      661 gagacagtct cattagctaa tgaatactgg catccagaaa cgccaccggt ggttatgttg
      721 ttctctcagg ttggaaaaac gattgtaaaa aatttttatg aaatggggga tattgaaaga
      781 gaaaaaatat ttaaacatgt cgaagatggt atggtctcgc ttgatgatga attggccaca
      841 gttgtagcta cagggcttat agaggcaata gtcactgcga ccgatagcaa ttctttactt
      901 tggcaagatt taagtaaatt tctgggtgac aaatcaaagt ctcatgcatt agtgtggaaa
      961 aattttggtc aggagtgata ttattcaaga agtattaagc tcgctattga gatcttaatg
     1021 cttcttgaga ttaaattaaa gttaggacga agtaattgca ggtggaaata aatccatagt
     1081 gacgtgtggc aatggctatt agatgttatg gtgtgatggg ctttggtgat ttaagagcta
     1141 agttagattg ccttatgaaa aagtatggtt atatatttaa tgatgtgtat catatattta
     1201 aataaaaaac ggttctctct tgtcaagata gtaatagg
//
LOCUS       DAZOYL010000511         1237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZOYL010000511 DAZOYL010000000
VERSION     DAZOYL010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.515"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            371..604
                     /locus_tag="ACHUOY_004225"
     CDS             371..604
                     /locus_tag="ACHUOY_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR0684801.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            662..1072
                     /locus_tag="ACHUOY_004226"
     CDS             662..1072
                     /locus_tag="ACHUOY_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR0684802.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
BASE COUNT          426 a          274 c          232 g          305 t
ORIGIN      
        1 aaacagagaa aggaaacgac agaggccaaa aagcccgttt tcagcacctg tcgtttcctt
       61 tcttttcagg gggtgtttta aataaaaaca ttaggttacg gcgaagaaga acggaaacgc
      121 cttaaaccgg aaaattgtca taaatagcga aaacccgcgc gccttccgcc ccgtaacctg
      181 tcggatcgcc ggaaaggacc cgcaaaatga taataattat catctacatg tcacaacgtg
      241 catctacgcc atcaaaccac gtcaaataat caattatgac gcaggtatcg tattaattga
      301 tctgcatcaa cttaacgtaa aaacaacttc agacaataca aatcagcgac actgaataca
      361 gggcaacctc atgtcaacga agaacagaac ccgcagaaca acaacccgca acatccgctt
      421 tcctaaccaa atgattgaac aaattaacat cgctcttgtt caaaaagggt ccgggaattt
      481 ctcagcctgg gtcattgaag cctgccgccg gagactgtgc tcagaaaaaa gagtttcgcc
      541 tgaagcaaac aaagaaaaga gtgacattac tgaattgctc agaaaacagg ttagaccaga
      601 ttgaagcaat ttagataatc gtgcagacta cgccccctca tatcacatgg aaggtactac
      661 aatggctcag gttgccattt ttaaacaaat attcgataaa gtgcgaaata atttaaacta
      721 tcactggttt tattctgaac taaaacgtca caatgtctca cattacattt actatttagc
      781 cacagagaat attcatcttg ttcttgaaaa cgataatacg gttttaataa aaggacaggg
      841 taaggttgta aatgtaagat tttcaaaaaa taaatgcctt atagaagcca ccttaaaagg
      901 attcaaatca ggagagttat cattttacga atacaggaaa aatcttgcta cagcaggggt
      961 tttcagatgg attacaaata tccacgaaaa caaaaggtat tactatacct ttgataattc
     1021 attactcttt actgagaaca tacagaacac tacacaaata tttccgcact aaatcataac
     1081 gtccggtttc ttccgtgcca gaaccggact cgctggcatg atgaaatatg tgtacccggt
     1141 aaccccggtg tgcatcgttt ttgattattc ccgcacactc gcgcagaagg agttccccgt
     1201 cgggctacgg tctctgttaa tacgggaata cggcgac
//
LOCUS       DAZOYL010000512         1236 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZOYL010000512 DAZOYL010000000
VERSION     DAZOYL010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.516"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..89)
                     /locus_tag="ACHUOY_004227"
                     /pseudo
     CDS             complement(1..89)
                     /locus_tag="ACHUOY_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(276..705)
                     /locus_tag="ACHUOY_004228"
                     /pseudo
     CDS             complement(276..705)
                     /locus_tag="ACHUOY_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(753..1118)
                     /locus_tag="ACHUOY_004229"
     CDS             complement(753..1118)
                     /locus_tag="ACHUOY_004229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684803.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCMEREFATIPAGISADG
                     MYCPECGTVHWPDGVIPPF"
BASE COUNT          303 a          360 c          250 g          323 t
ORIGIN      
        1 agcacgtgat ctccgaacat ttctccccag tctgcgaccc ctttgtttga cgtcagtatg
       61 atgctcgctt tttcatatcg acagttcatc gataggaatt taaatcccca aaagattaaa
      121 aaagcaccga agaatgcgcg tttattcaac acaacctgat catcaagtaa tcggtataga
      181 cacttaatat tgcttacatt gattgattca atgtatctct gatggttttt tgctctgttc
      241 ccctcaacat ggacgcaact tcccctctgc gactctcagc cgcaccaccg tattcgagcc
      301 agcggctcat gcatcataac ctgctctgcc agacggtaac ccctcttcag ccccgtaaac
      361 cgcatctgac ctccgcacag ctcgcacttc agcgggtcaa ccttcagtca ctgctgatac
      421 atccctctcc ttgtgatttg cctcgccgtt tttctcactg tctccgttat ggcgtacacc
      481 acatcttcca gtaatcgccg cttcaccgga ctcaggaccc cgtatcacca tccggaaccc
      541 cttatccgcc acatgccagg agaacctttc cataaactcg tcgccactca tcacaaggtt
      601 ttcttctcgt tttgtccggt ggctgttgta acgcaggcca atttcatcct gaccggcata
      661 atgttccagg cggctcatct gcaccgactg cttctccagc cagttaagcc atcggtttcc
      721 ctgctgaaaa atatcggcaa aacggggaag catcagaaag gcggaataac gccatccggc
      781 cagtgaaccg taccgcattc agggcaatac atgccgtcgg cgctgatacc ggcaggaatt
      841 gtcgcaaatt cccgctccat gcagcgggca atttccggat acccttcgtc atcaacacgt
      901 acaaaccaga agaccagttt tttgtttccg gcatccacaa agaacggaat attcaggtct
      961 gcgcaacact caacggcatc gtcaaaatta tcaaagcgca gaacctcagc ttcttcttcg
     1021 tcaaaaaaat catcttcgtg aagttttacg acatagcgga ggagtttgct tctttgaggt
     1081 gcaggttttc gtttgcgtgg tttagctgaa cgggccatat aactacctga aagacaatga
     1141 tattgtctgt ttttataacg gtaatcacag gccatgacaa gccgcagccg tcaggccgcc
     1201 tactcggggc ggtttagaac gcttatttag taccag
//
LOCUS       DAZOYL010000513         1234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZOYL010000513 DAZOYL010000000
VERSION     DAZOYL010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.517"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..761)
                     /locus_tag="ACHUOY_004230"
                     /pseudo
     CDS             complement(1..761)
                     /locus_tag="ACHUOY_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          308 a          299 c          309 g          318 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcaagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgccg gatacaggga aacccataac cacccgatac gggggaacta atcgcttttt
      901 aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt taaaaagggc
      961 ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat ggagtgttgt
     1021 ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag
     1081 gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc ttttaaccac
     1141 gtcaggcgag gtggtatcct tcttatcccg aataaccaag aaggaaatct atatgactaa
     1201 agaagaattt gtctcttata tttttgataa aacg
//
LOCUS       DAZOYL010000514         1226 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZOYL010000514 DAZOYL010000000
VERSION     DAZOYL010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.518"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..1151)
                     /locus_tag="ACHUOY_004231"
     CDS             complement(<1..1151)
                     /locus_tag="ACHUOY_004231"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0684804.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          290 a          327 c          325 g          284 t
ORIGIN      
        1 tctttcagtt tgaagcaaac cgccgggatg ccttcgtccg ggcgaccggc acagatgaac
       61 tcatacggcc ccagtttggc gatctcatcc gccagatagg cagcgacctg gtaggaagca
      121 ttctgtactt tggtatagcc ttcacgacca aggcgcagga attcatagta ctgtgcaatc
      181 acctgacccg ccgggcggga gaagttgatg gcaaaagtac caatctgacc accgaggtag
      241 tcaacgttga acaccagttc ctgcggcagc gcttcttcgt cacgccagat aacccagccg
      301 cagcccagcg gagccagacc gaatttgtgg cctgaagcac tgatcgattt cacacgcggc
      361 aggcggaagt cccagacgat atccggggcg acgaacggtg ccaggaagcc accgctggcg
      421 gcgtcgatgt gcatgtcgat gtcgataccg gtgtcggcct ggaatttatc cagtgcatcg
      481 tgcagcggtt gcgggaactc atagttaccg gtgtaggtca cgccgaaagt cggcaccacg
      541 ccgatggtat tttcgtcgca ggcttcaatc atgcgtttcg ggtccataaa caactgaccg
      601 gggcgcatag ggatctcacg cagctccaca tcccagtagc gggcgaattt atgccagcag
      661 atttgtaccg gaccgcacac caggtttggt ttatccgttg gtttgcctgc agcttccata
      721 cgcttgcgcc aacgccattt catcgccatc ccgccgagca tacaggcctc ggaagaacca
      781 atggtgttgg tgccaacggc ctgaccattt ttcggcgcag gcgcatgcca cagatcggca
      841 accatattta cgcaacgcag gtcgatggct gcggattgcg gatattcttc tttgtcgatc
      901 cagtttttat tgatcgacaa atccatcaat ttatggacgt tttcgtcgtc ccaggtctgg
      961 cagaaagtgg ccaggttctg acgagcgttg ccatcaagat ataattcatc attgataatc
     1021 tggaatgcga catcatcgcg catttcgtgc agcggaaatc gttttgactc cgcgatagta
     1081 gaaatggcct ttgcgccaaa acgtgaatcg agtagttctg agcggaaatc cgttaacagc
     1141 ttctggtcca tttcgaactc cttaaattta tttgaaggca ataaaaaagt aggatttatc
     1201 cgcaatggaa gcaaggcatt acaggc
//
LOCUS       DAZOYL010000515         1216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZOYL010000515 DAZOYL010000000
VERSION     DAZOYL010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.519"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            194..>1216
                     /locus_tag="ACHUOY_004232"
     CDS             194..>1216
                     /locus_tag="ACHUOY_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0684805.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKH"
BASE COUNT          351 a          265 c          276 g          324 t
ORIGIN      
        1 taatttagca tttcaatcat ctgacatcat tataaaaaat gagattcaca taaagataaa
       61 attattgttc tcaaggaaag catgaagggg cttcgtaagc aatattcatt cggtgccaag
      121 acaggacttg atacacagaa tctcaacttt tatgctctca gactatatca ccacatgttt
      181 tacaggctca gaaatgaacg atagattatg ctttgaagtg cacgataaca agggttattt
      241 cgtatttccg gatacctggt ttggcccatt gctgggggag tttgaagaag tgcttgatgc
      301 atacgacgct gatgaaatca gcgagacgag ttacatcaac aaactccgac gccttgctca
      361 acgggagccg gactttattg atatacacgc tcatctggca tatgcatttc tggaacagaa
      421 tgccccgcgt aaggcactga acgcctctct taaaggactg gcggcaggta atcgtatcat
      481 cccggaaagt ttctgtggtg aaataatctg gatgcatccc gaaaaccggc cttatctgcg
      541 cgcgctttat gcagcaattc tcgcaaatgt gcacttacag cgccatcagg atgcggttat
      601 gctcactgat aaaatactgg catacaatcc tgaagataat cagggggctc gctggttgtt
      661 gggttcagag ctgttacgca ccggcgatca tgagcgagcc ttcagtgtac tgaaagagca
      721 tgctgatgaa ttttcacctt actggtatga actcggtctc ctgcattttc tgaacgggga
      781 acatgttaag gccgccacag cttttcgcca tggctttgcc acaaacactt acatcgcaga
      841 aatgctgtgc ggaaatctgc atccatttcc acttgctgtg tggcatgact tctccggctc
      901 tctggatacg gcagaagatt actatgcaac atactcacca ttatggggac aatatccgga
      961 agcattgctg tttgtaaact ggctctataa ccattcctcc gtgctgcatg aacgatcaga
     1021 aatcattaaa tgcgccgaaa tgctgattca ggaagatgat ttcgaaatat gcgaaagtat
     1081 tttaagacag caggagcacc tgtggaaaag aattgacaag acactttctg aggaaattgt
     1141 acagaaatgc agaaatatga acggtgaata tatctggccc tggatattgc ccttttccgc
     1201 agcagggatg aaacat
//
LOCUS       DAZOYL010000516         1210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZOYL010000516 DAZOYL010000000
VERSION     DAZOYL010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.520"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            54..1210
                     /locus_tag="ACHUOY_004233"
                     /pseudo
     CDS             54..1210
                     /locus_tag="ACHUOY_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          321 a          305 c          272 g          312 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca
//
LOCUS       DAZOYL010000517         1210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZOYL010000517 DAZOYL010000000
VERSION     DAZOYL010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.521"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            74..622
                     /locus_tag="ACHUOY_004234"
                     /pseudo
     CDS             74..622
                     /locus_tag="ACHUOY_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            696..815
                     /locus_tag="ACHUOY_004235"
                     /pseudo
     CDS             696..815
                     /locus_tag="ACHUOY_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            948..1118
                     /locus_tag="ACHUOY_004236"
                     /pseudo
     CDS             948..1118
                     /locus_tag="ACHUOY_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          348 a          266 c          317 g          279 t
ORIGIN      
        1 gagccggatg agctgagagg ttcacgtccg gttccgtgag caccagaggg ggaggttccc
       61 tcgggtgact cgacttgtag tacattgttc acgaatgagt gatgcaacaa ggctgaagaa
      121 caggttatcg gtgcgcttct cggaagtcgg gttggtcctg aacgcaggga agacgaatat
      181 cgcctacatt gacacgttta aaaggagaaa cgtcgctacg agtttctcct ttctcggata
      241 tgacttcaaa gtgcgtactc tgaagaattt caaaggcgaa ttgtacagaa aatgtatgcc
      301 gggtgcatca aatgcagcaa tgtgcaaaat aacagaaaca atcaagaagt ggcgtataca
      361 tcgctcaaca gctgagagtt tgctggattt tgcgaggcgc tacaatgcga tagtgagagg
      421 ctggattgag tactatggaa agttctggtc cagaaacttc agctatcgac tgtggagtgc
      481 aatgcagtca cgtttgctta agtggatgca gtctaaatac agactttcga accggaaggc
      541 tcagcgaaag ctggcgctgg taaggaaaca gtatccgaag ctatttgccc actggtattt
      601 actacgtgca tcgaatgagt gatcaagagc cgtatgacgc gagagtgtca cgtacggttc
      661 cgtgagcagc cggagagtga aactctctcc ggcggggttc cgatagcggt ggcgaacatg
      721 cggcggtgtt gtactcgctg atcggcacat gccgtctgaa caatgtggag ccagaaaaat
      781 ggctgcgtta cgtcattgag catatccagg actgaccggc aaaccgggta cgcgatctgt
      841 tgccctggaa agttgatctg agctcttagt aaatatcaat acggttctga cgagccgctt
      901 acgcagatca tgccataatg aataaacttt aatcagcgaa attgacgaag aagctggcag
      961 cgctccaaca tcagttaagc cgcaaggtta aattcagcaa caactggcag aagcagaaac
     1021 acaaaataca gcaactgcat tcccatatcg caaacatccg cagagactac tttcacaaag
     1081 tcacagcgac tgtcagcaaa aaacacgtga tgatcgtcaa ttacgttatc actgccaact
     1141 gacttcacct tttggttcat atgaagcagc atccagcggt gaatgttccc ggatatattc
     1201 gataagtact
//
LOCUS       DAZOYL010000518         1181 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZOYL010000518 DAZOYL010000000
VERSION     DAZOYL010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.522"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            184..456
                     /locus_tag="ACHUOY_004237"
     CDS             184..456
                     /locus_tag="ACHUOY_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR0684806.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(422..829)
                     /gene="nudG"
                     /locus_tag="ACHUOY_004238"
     CDS             complement(422..829)
                     /gene="nudG"
                     /locus_tag="ACHUOY_004238"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR0684807.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            916..>1181
                     /locus_tag="ACHUOY_004239"
     CDS             916..>1181
                     /locus_tag="ACHUOY_004239"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0684808.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
BASE COUNT          287 a          295 c          302 g          297 t
ORIGIN      
        1 tgtgcttttg cagttttcga ctataccatc aagaatgtga cggtaagcaa gaaaaaaatg
       61 acccgggaaa gcaaattaat aacgagagta atctcataac caaatgcgat aactcagacg
      121 ctaaagtttt gccacccggc taccgttatc agttgcagag acaataacgt taaggagttc
      181 cctgtgagtc gagcattgtt cgccgtagta ttagcatttc cattaattgc actggctaac
      241 ccgcattatc gtccggacgt ggaggtaaac gtaccgccag aagtgttcag ttcaggcggg
      301 cagtcagcgc agccctgtac tcagtgctgc gtttatcagg atcaaaatta ttctgaaggg
      361 gcggtaatta aagccgaagg aatactgctg caatgccagc gggatgacaa aacccttagc
      421 actaatccgc tgctctggcg gcgcgtaaag ccataaacgc ctctaatagt ggaatgtcag
      481 caggggccag cggatattgc agcgcctctt caggtgagca ccagaccagc gcctgatgtt
      541 catgtgcctg taacgtcccg tggaagtcgg gtacgtgcca ggcatgaaga tggataatcc
      601 ggcccgaaac ttctcgctga tggctggcaa catactcacc cacagttgct tcgatgccca
      661 attcttcgtt taactcacgc accagtgcct gctgctggct ttcatccagc tcgactttac
      721 catcggcaaa ctcccataat cccgcctgat cgctctgggc ggggcgttgc gcgagtaaaa
      781 ttttgccatc acgttcaatg atggcggcaa caacttcaat cattttcata aattacacac
      841 gggcagataa gcgaaaaatg ccataatatc cgcactgttg ctcaatcaga aaggctttat
      901 atttaaggag tgcctgtgct agaccgccat cttcatcccc ggattaaacc gttgttgcat
      961 cagtgcgtgc gggttcttga taaaccgggc attacgccag atggtttaac attagtggga
     1021 tttgccatcg gcgtgctggc tctgccgttt ctggcgctgg gctggtatct ggcggcgcta
     1081 gtcgtcattt tgttgaacag gctgcttgat ggtctggacg gtgcgctggc gcggcgcaga
     1141 gagcttaccg acgcgggcgg ctttcttgat atttctcttg a
//
LOCUS       DAZOYL010000519         1160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZOYL010000519 DAZOYL010000000
VERSION     DAZOYL010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.523"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            247..492
                     /gene="yahM"
                     /locus_tag="ACHUOY_004240"
     CDS             247..492
                     /gene="yahM"
                     /locus_tag="ACHUOY_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR0684809.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(509..>1160)
                     /locus_tag="ACHUOY_004241"
     CDS             complement(509..>1160)
                     /locus_tag="ACHUOY_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR0684810.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          351 a          277 c          275 g          257 t
ORIGIN      
        1 tttcgcagcc atgcggtgta taaaaaatga tctcatgcag atgttttgtg aatgtgtggg
       61 ttgacattca tatgaaaaaa atcataattc catcatgttt gtgtggggtc ttttctgtac
      121 cttacgcatc gcactcaagc ctgacagaaa atatgctgta aggctcatat caaaaaccgc
      181 cattagctca tcaggaagag cagacgacaa ccaggattgt tgtatggtac gggggtcgag
      241 gcctcgatgg cggtccaact tttcaaaact cttctgaatc agatcccctt attatcatcc
      301 ctgcaaagtg gaacattgcc attgtttggc tattcaggac gggtcagacc aatgaaaaaa
      361 gcccacacag gggagagtgg gctgaaatgg gaagctaaag actcaagtaa acttatcgga
      421 aataaggacc acgcattacg ggggctatca tcgccgatag tcgggattcg gcaaatcagg
      481 ttgattacgt agagagtaaa ttatctgctc accgctgcgt caccccttcg taaatcaggc
      541 gtagcgcgaa tacaccaata attgcaccaa taacccgact ggcaacgcgt tgcatacgcc
      601 cataagcacg acgcacagcg ggcaaagaaa acgcctgact aagaaagact cgccagataa
      661 ctgatgcgag cacaatcccc gcccaggcca ttaaacgtgc ccatgttggt gtttcggcat
      721 ttaatgttac tgagaaaata ctgataaaaa ataaaacggt ttgcgggtta gagagatcgg
      781 taattaatcc gcggcgaaaa aagacatacc aggaggcgct aatcggttgt tgtagtgtgc
      841 tcatttgcgg tgttgactgg cggcgcatgc tgcaccacgc aaaccataag agataagcgc
      901 cgccgacgat tctgataagc gaaaaaatct cctcacactg cgttattagc gttgcaagac
      961 caaacaaacc caacccggaa taaaatgcat cgcccagcgc cacgcccagt ccggtcagca
     1021 cccctgcgcg tcgaccggaa gccaggctgg tttgtactac cacaaagaga ttggctcccg
     1081 gattaaaaaa agtaatcacg aacagtccta cggtcaggta aacggcgtgc aaaggatcca
     1141 tagtgatttc atccataaat
//
LOCUS       DAZOYL010000520         1157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZOYL010000520 DAZOYL010000000
VERSION     DAZOYL010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.524"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHUOY_004242"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHUOY_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="ACHUOY_004243"
     CDS             572..>1157
                     /locus_tag="ACHUOY_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0684811.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          321 a          260 c          276 g          300 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc ctggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAZOYL010000521         1149 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZOYL010000521 DAZOYL010000000
VERSION     DAZOYL010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.525"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            101..877
                     /gene="exuR"
                     /locus_tag="ACHUOY_004244"
     CDS             101..877
                     /gene="exuR"
                     /locus_tag="ACHUOY_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR0684812.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
BASE COUNT          328 a          283 c          260 g          278 t
ORIGIN      
        1 aatacattaa ttcaatcacc aaatttatat atgaccaatg gcatacccat gaataaagtg
       61 gtatgataac ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg
      121 ccgtttgtat caacaacttg ccgctgacct gaaagagcgc attgaacagg gcgtctatct
      181 ggtgggtgat aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac
      241 ggtagttcgt gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg
      301 ttcaggtatt catgtggttt ccaaccagcc gcgccatcag caggcggctg acaataatat
      361 ggaattcgcc aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa
      421 tattgccgag ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat
      481 tcaggaacag gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca
      541 cattcaggta gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg
      601 gacccagcgt agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac
      661 cgttgataac tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc
      721 ccatgctgct aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa
      781 cgaaaccagc gacgatttcg agttcaatgc cgaccgctat ctattcgccg aaaacccggt
      841 agtgcatctc gatacggcca ccagtggcag taaatgaaac tcctgtccga caggcgctgc
      901 gacgcgcctg ttcgcttcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc
      961 tctcgccacc ctcccctgca ttcagcaaaa tcagactcca cggacatgta attttgataa
     1021 cgaacaacgt tgacctttgt tacaattaga ttcaatttga atttatgttt ttgaatgctt
     1081 tcttatctca cgatttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga
     1141 acataccaa
//
LOCUS       DAZOYL010000522         1148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZOYL010000522 DAZOYL010000000
VERSION     DAZOYL010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.526"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUOY_004245"
     CDS             <1..122
                     /locus_tag="ACHUOY_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684813.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHUOY_004246"
     CDS             119..376
                     /locus_tag="ACHUOY_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684814.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHUOY_004247"
     CDS             376..750
                     /locus_tag="ACHUOY_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0684815.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="ACHUOY_004248"
     CDS             747..>1148
                     /locus_tag="ACHUOY_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0684816.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAZOYL010000523         1143 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZOYL010000523 DAZOYL010000000
VERSION     DAZOYL010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.527"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            336..760
                     /gene="yqeK"
                     /locus_tag="ACHUOY_004249"
                     /pseudo
     CDS             336..760
                     /gene="yqeK"
                     /locus_tag="ACHUOY_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            796..876
                     /locus_tag="ACHUOY_004250"
     CDS             796..876
                     /locus_tag="ACHUOY_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684817.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(910..>1143)
                     /gene="yqeJ"
                     /locus_tag="ACHUOY_004251"
     CDS             complement(910..>1143)
                     /gene="yqeJ"
                     /locus_tag="ACHUOY_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR0684818.1"
                     /translation="RRNIEYTWIENYDTAHLTSKKVNKFEIMDQVDDDRLAQLIPDFY
                     VFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR"
BASE COUNT          371 a          174 c          190 g          408 t
ORIGIN      
        1 atcgctcatt tttacttttt ttaccataat gttactcagt agcatcggca atttacataa
       61 cttacacgtt gttttttata tctcgaaaaa acagatagat agtgtgcgca ctcgaaatga
      121 tattggtaac ataaatagct ttattcgtga taaacatctc aaaagatttc ctttattata
      181 ttcgaaattc atttgatcaa ggttatcacc ttatggagac gttacgtaga atgactatat
      241 ttgttatcgg cacattaggg gtattaattt tagtattgat ggaaggtgat tattgaacag
      301 taacatcgag tcacggtaaa aatgtgaggt atacaatgga cattgagttt tcgcagattc
      361 atgaaatggt ctatatgcat gatattgtaa attcggactc gaaaaagaaa ccaaggattc
      421 cgttaaaaaa attccttaat gctgagaatg ttctcacgca aacaacatca tggacattaa
      481 attcaagata tgtgaatgtt aattctgtca ataaagtcaa cgtaaagagt aaagttaaaa
      541 acagttatat ctcacgatct gtcaatgatg agtttagttt aacagatgat gaaatcaatt
      601 cttttaaaga aacgttagtc ctttcctcta ttgattcctg agtaaactgg ttcttaataa
      661 tcctctttct gtattgttta catcgactgt acgaaggaat aataatcgag ctaagatgaa
      721 tgtcgagttc gactcatgga tttgcaccag atgttgttga tacatgatat tttacattgc
      781 aaggagatgt ctaaaatgaa agatgttgat caaaactttg atgctttaga ctgccatata
      841 ctgcgagaat atttaatttt attgttttat gattaacgag gttccatctt ttttgttatc
      901 tattttagtt taccttgcac aatgcattat tgctctgtta ccaatgctca aaataaaagc
      961 atgcttacct tgctttagta tattatagct tacacttttt tctggaaaga cataaaaatc
     1021 aggaataagt tgtgcaagtc tatcatcatc aacttgatcc ataatctcaa acttattaac
     1081 tttctttgat gttaaatggg ctgtgtcata gttttcaatc catgtgtatt caatatttcg
     1141 ccg
//
LOCUS       DAZOYL010000524         1141 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZOYL010000524 DAZOYL010000000
VERSION     DAZOYL010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.528"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            14..736
                     /gene="sdiA"
                     /locus_tag="ACHUOY_004252"
     CDS             14..736
                     /gene="sdiA"
                     /locus_tag="ACHUOY_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR0684819.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(803..1027)
                     /gene="yecF"
                     /locus_tag="ACHUOY_004253"
     CDS             complement(803..1027)
                     /gene="yecF"
                     /locus_tag="ACHUOY_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR0684820.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1014..>1141)
                     /gene="yecU"
                     /locus_tag="ACHUOY_004254"
     CDS             complement(1014..>1141)
                     /gene="yecU"
                     /locus_tag="ACHUOY_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR0684821.1"
                     /translation="DVTLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
BASE COUNT          297 a          247 c          276 g          321 t
ORIGIN      
        1 gttgcggttt actatgcagg atacggattt tttcagctgg cgtcgcacga tgctgttgcg
       61 ttttcagagg atggaggccg cagaagaggt ctaccatgaa atagagcttc aggctcagca
      121 gctggagtac gattactatt cgttatgtgt tcgccacccg gtaccattca cgcgacctaa
      181 agtggctttc tacaccaatt accctgagtc gtgggttagt tattatcagg caaaaaattt
      241 tctcgcaatt gatcctgtgc tgaaccctga aaactttagt cagggccatt taatgtggaa
      301 tgatgactta ttcagcgaag cacagccatt atgggaagcc gcgcacgcac atggtttacg
      361 ccgcggtgtc actcagtatt taatgctgcc aaatcgggcg ctgggctttt tgtccttttc
      421 ccgttgcagc acgcgcgaaa tacccattct tagtgatgaa ctgcaattaa aaatgcagtt
      481 actggtgcgc gaaagtctga tggctctgat gcgtttaaat gatgaaatag tgatgacgcc
      541 agagatgaat ttcagcaagc gcgaaaaaga aattctgaaa tggacggcgg aagggaagac
      601 atcagcagag atagcgatga ttttgtcaat ctctgagaat acggtcaatt tccatcagaa
      661 aaacatgcag aaaaaaatca atgcaccaaa taagacccag gtggcctgtt acgcggccgc
      721 tactggctta atttgatcgc ttttctttct gcgtgccaga tgcaaaaatc ggctgaaagg
      781 cacgctctca gccggtttta tattactgac ggtaggcttg tttaatttgc ttaaccgttt
      841 tggaaaatac tgcagcctgg gtttcgtctt cgatcagcgc aagctgtttt tccatcttta
      901 acatcacgcg gcccgcgtca gcttgtccca tcgcctgtag catcagcgtc agcatcgctt
      961 tcaggcagga gacttcattt gccagttctt gattattctc ggcagtagaa aaatcaggcg
     1021 tactcattta ttttcctcgt catgttgcaa tgaaaatttg cggtgaaaaa tgttaaggcg
     1081 gcggagtata ccataagctt tgctaaaaat agcagtggtt gttttttgag cgtgacgtca
     1141 a
//
LOCUS       DAZOYL010000525         1119 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZOYL010000525 DAZOYL010000000
VERSION     DAZOYL010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.529"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..478)
                     /gene="umuC"
                     /locus_tag="ACHUOY_004255"
     CDS             complement(<1..478)
                     /gene="umuC"
                     /locus_tag="ACHUOY_004255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0684822.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(478..897)
                     /locus_tag="ACHUOY_004256"
     CDS             complement(478..897)
                     /locus_tag="ACHUOY_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0684823.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          315 a          292 c          236 g          276 t
ORIGIN      
        1 tctgccgctg ccattttttt gccgcatgat tggcaagctt agccagcgtt ttggtctggg
       61 cgatccccat accaacagta agatgggtac gttgtagcac cgttgcgcga atttctctgc
      121 caaaatcagt cagatcgcga caattacgca cacctgtcag atcgcagaat gcctcatcaa
      181 tactgtaaat ctcgacgcgg ggcgatagtt cttccagcgt cgacatcacc cgattgctca
      241 tgtctgcgta aagctcataa ttgctgctaa agcaaaccac accacagcga cgaaacagat
      301 ctttttgttt gaaccaggga tcgcccattt taacacccag cgcctttgcc tcagcgtttc
      361 gggcgataac gcaaccgtca ttattcgata gcacaaccac cggtttaccc cataaatcag
      421 ggcgaaacac cgtctcacag ctggcataaa acgcgtttac atcacagaga gcaaacatca
      481 gcgcatcgcc ttaacgacgt ggatcaccac accaaagaca tccagcgtat cttcgctact
      541 gatggtaatg ggcgagtacg cactgttcat gggaataagc tgtaccgtcg ggcgtagttg
      601 caattttttc accgtaaact cgccgtcaac agcagcgatg acaatatcac catggctggc
      661 ggtaatagcg ctatcgacaa tcagtaaatc accgtcacta attccaccat caatcataga
      721 atcaccactt gctttgacga agtaagtcgc gctgggatgc tggatcaaca gttgattcag
      781 atcgatgcgc tgttcgacgt aatctgctgc cggtgaagga aagccacact gaacaagatc
      841 gctaaatagc ggaaaagtca caatttcgcg gagatccgca ggcttgataa acaacataat
      901 aatctgcctg aagttatact gtttttatat acagtaatct gttcttgcca gcagatcaat
      961 actgattcag gctatcaata tttgtcgctg catagactgc tgatttttcg ttctcttatc
     1021 ctgtgctcac gtggccttct ggcgacgacg ctcatccagc agaaatgaaa aatatcaccc
     1081 ggcaaaaaaa tagaatagaa gcattcgcca taatgacat
//
LOCUS       DAZOYL010000526         1118 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZOYL010000526 DAZOYL010000000
VERSION     DAZOYL010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.530"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1000
                     /gene="htrE"
                     /locus_tag="ACHUOY_004257"
     CDS             <1..1000
                     /gene="htrE"
                     /locus_tag="ACHUOY_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR0684824.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1017..>1118
                     /locus_tag="ACHUOY_004258"
     CDS             1017..>1118
                     /locus_tag="ACHUOY_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0684825.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATV"
BASE COUNT          335 a          221 c          266 g          296 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggaa cgtcaaatga
     1021 taaaaataac gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat
     1081 ttgcaacgga tattaatgta gagtttacag ccactgtc
//
LOCUS       DAZOYL010000527         1116 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZOYL010000527 DAZOYL010000000
VERSION     DAZOYL010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.531"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHUOY_004259"
     CDS             84..1115
                     /locus_tag="ACHUOY_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR0684826.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          300 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagc
//
LOCUS       DAZOYL010000528         1114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZOYL010000528 DAZOYL010000000
VERSION     DAZOYL010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.532"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..318
                     /locus_tag="ACHUOY_004260"
     CDS             <1..318
                     /locus_tag="ACHUOY_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0684827.1"
                     /translation="MIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSR
                     FEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSKMRKYLQSINECMLLIKNIV
                     HQD"
     gene            552..1114
                     /locus_tag="ACHUOY_004261"
                     /pseudo
     CDS             552..1114
                     /locus_tag="ACHUOY_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          396 a          204 c          194 g          320 t
ORIGIN      
        1 atgataaaaa atttgcgctg tgaagatatt atgcctggcc gatggaccac tcaacttgtc
       61 aataagcact taggttacag atacacagga gtattcaaaa ccctggcaag tattgatgac
      121 aaaccgtccc gtttcgaaat tttgatacca ttggtacaaa ctttagttcg cgataatgta
      181 aagctaaata atgatgtgta taaagaactt aataagttta tgcacgatta cgacaaaaca
      241 tcatcaaaaa tgagaaaata ccttcagagc atcaacgagt gcatgctcct tataaaaaat
      301 atagttcatc aggattaaaa cactcaaaga aagaggtaat tttagaatga aattatctgt
      361 gatgattatt aatacactgc agagaaataa aaaacaatct gaagcatgct ctgaaacatc
      421 ctactctgtc actctaatga tggcaccttg ctcttcttat aaagaaggga acaattaaga
      481 atattaaatt ttcaaacttt catcaacttc acatcaagaa attaaaaaat ttaaacatta
      541 atctaaataa taacaacctg atcacataat cgggtacgat tttatccgat aactataatg
      601 ctataactct gggaaatggt tggaatacac cgctgggtgc catctctttt tacgctgccc
      661 gatcgagtag taagttaaat aatgatacca gacacgaagg tacgagctat caggtcgcct
      721 ataataaata tctagtacaa acagccactc gatttagcgt ggccgcctgg cgttatgctt
      781 cgcaggatta caggacattc agcgaccatc tttacgaaaa tgataaacac tatcatcaga
      841 gtgattatga tgatttttat gatattggca gaaaaaatag cctttctgcc aatattatgc
      901 aacctttatc caataatctg ggaaatgtat cattaagtgc gctttggcgg aattactggg
      961 ggcgaagtgg aaatgctaaa gattaccaat tcagttactc caataactgg caacacatta
     1021 gttatacttt ctctgccagc caatcttatg atgaaaataa taaagaagag gagcgtttta
     1081 atctgtttat ctccattcct ttctactggg ggga
//
LOCUS       DAZOYL010000529         1113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZOYL010000529 DAZOYL010000000
VERSION     DAZOYL010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.533"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..83)
                     /gene="yqcG"
                     /locus_tag="ACHUOY_004262"
     CDS             complement(<1..83)
                     /gene="yqcG"
                     /locus_tag="ACHUOY_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR0684828.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLV"
     gene            222..893
                     /gene="queE"
                     /locus_tag="ACHUOY_004263"
     CDS             222..893
                     /gene="queE"
                     /locus_tag="ACHUOY_004263"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR0684829.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          309 a          227 c          251 g          326 t
ORIGIN      
        1 aaaactaata cagaaaaaat aaatataatt ttggtgaatg ttttgacatg attaaatcca
       61 ttttctttat tttcttctga cataatacgt tactcccttt aattattaac gatatgtttc
      121 gcatttttct ttaaatctgc ctgtaacaga atgttaataa gacatgattg ttgtggcagg
      181 atctgcaata attaacaatt atccacttca cagagaatgc tatgcagtac ccgattaacg
      241 agatgttcca gaccctgcaa ggtgagggtt actttaccgg cgtccccgcc atttttattc
      301 gtttacaggg atgcccggtt ggctgtgcct ggtgcgacac caaacacacc tgggaaaagc
      361 ttgaggatcg ggaagtctcc cttttcagca ttctggcgaa gaccaaagag agtgataagt
      421 ggggggcggc gagcagtgaa gacttgttgg ctgtcattag tcgccaggga tacaccgcgc
      481 ggcatgtggt gattacgggt ggtgagcctt gcattcatga tttgctacca ctgactgatc
      541 tgctcgaaaa gaacggtttt agctgccaga tcgaaaccag tggtactcat gaggtacgct
      601 gcacaccgaa tacctgggtt accgtatcgc caaagctgaa catgcgcggc ggctatgacg
      661 tgttgtcaca ggcactggag cgagccaacg aaatcaagca tccagtgggg cgcgtacgcg
      721 atattgaagc actggatgaa ctattggcaa cgctgaccga tgataaaccg cgagtcattg
      781 cactgcagcc gattagccaa aaggatgatg ccacacgttt gtgcattgaa acctgcattg
      841 cgcgtaattg gcgtttgtcg atgcaaacac ataaatatct aaatattgcc tgattaaaca
      901 tttataagcg ttataaatgg gtggaacctg taaagacttc tactcattta tattgtttgt
      961 cgcctctgaa aactcctcca ttttacccat ccagggctaa tcattagcat tctctacaaa
     1021 ttctgtggca ttaatttttc gctggagtga aaattattgc ggtaaagttt ggtagatttt
     1081 aatttgtata gagttatttt aaatatttac ctt
//
LOCUS       DAZOYL010000530         1112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZOYL010000530 DAZOYL010000000
VERSION     DAZOYL010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.534"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(214..1068)
                     /gene="rclR"
                     /locus_tag="ACHUOY_004264"
     CDS             complement(214..1068)
                     /gene="rclR"
                     /locus_tag="ACHUOY_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR0684830.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT          314 a          280 c          245 g          273 t
ORIGIN      
        1 caaaaaaatc accgacaata atcacgtatc aagtaatagt cggggtttta tttaattgaa
       61 gcaatttagt ttgcattttt atttaaaaac tgagctagag tccatcagaa aaaaatatat
      121 aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg ctcacaactc ccggaatgcg
      181 gattatcttt gccctgattt ctggcggttt tattcagggt gcaagctgtc tgaccctttc
      241 ccgatattct cccggagtac aaccaaactc gcggacaaac gccttgtgaa aagatgattc
      301 actggcatag cctactgact cagcgatcac cacaacaggg agcatttccc gggaaaacat
      361 ctgggccgct atttgtagac gcaactttgt taatacagcc agcggcgtgg ttccggaaac
      421 atcacggaaa agctgggcaa aacttgcccg agacatgtgg gcgatgctgg ccagcgattc
      481 gacggtccag gcgtgtcctg gcatttccag catttgctgt attaccgcac caagacgtgg
      541 atgcagaagc aaactgagaa tgtttttctc tgtattaacc tgtgcaatcc actcacgcac
      601 cgcgagggta aagaatgtag cgcagatctg gctacacagt gcatccactc ccggcatcgc
      661 cgatctggat tcctgttgta aaaacgggat cgcctccctc agccagttat attccacgct
      721 gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac gttaaaaaat aacgcgccga
      781 atgttgcaac cgaagagtgc cgcagacaat acaggtcgat tcattatcga catgactcag
      841 gcgatgagct gaattttgtg gtagcaggac cacatttccc gggcgtaatg taaaaatctc
      901 tcccgtcggc atttccagct tcgccgctcc ttgcgttaac gcatgccaac gaataaccga
      961 taattccccg gcaccatgtt gaagctgcca gtcacttcct aacacgcaat tcttatcgat
     1021 cgttccttgt ggagcgttaa gcatcaacaa acggctaagg gcatccatat cagactcctg
     1081 agtggttttt gattacaaac accaacaatc tt
//
LOCUS       DAZOYL010000531         1104 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZOYL010000531 DAZOYL010000000
VERSION     DAZOYL010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.535"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            90..197
                     /gene="yniD"
                     /locus_tag="ACHUOY_004265"
     CDS             90..197
                     /gene="yniD"
                     /locus_tag="ACHUOY_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR0684831.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(250..1008)
                     /locus_tag="ACHUOY_004266"
     CDS             complement(250..1008)
                     /locus_tag="ACHUOY_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR0684832.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          302 a          272 c          262 g          268 t
ORIGIN      
        1 ccgacggctg taataacaaa aaatcccttg atgcctatcc cttttgttac actccgttat
       61 cacgcacaag agatatgcag gacactggta tgccgactaa acgctttgat aaaaaacact
      121 ggaagatggt ggtggtgcta ctggcaatct gtggcgctat gttgttgcta cgttgggcag
      181 caatgatttg gggctgagca gtgacaaacg aagacagcca gaccgcataa cacggtccgg
      241 cagataattt tacatgctgt agcccagcga taaggttgtc cgacgatcgg tatgttccgg
      301 tgcagactct ggaggttcag agttccatgt gacgttgtaa gccaccttca gtccaaaatg
      361 ttcattaata gcaacattta atgcgctttc ggagttcagc gttgtatctt ccgcgccaaa
      421 gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac tgccatgcat aagccccgga
      481 ggcatagccc agcggctggg tttcactggc attgtcggta tatttgtcgt aacgcacacc
      541 tgggccgaat tcaaagcgga aactgtgtac cgggccattc aaaaactggc gaccataacc
      601 cgcggtcaac acatcgcgct ctcgatagcc gttataacgg tctgtcagcc agcttgcctg
      661 accaaataaa tagtcataat cagttaaatt aaaacggcta cgcccgcccg ccgcatattt
      721 ttctgaagaa cgctcatcat tagaagaagt attactggcg ttcccccaca gcgaccaggc
      781 agtggtttgt ccataccagg tcatggtggt gtcagccgta agtgaggagc ttttcgtatt
      841 gcctgattgt gcaagatatc ctgcgttcag attaccttcg aaaggttttt tagcgctggc
      901 aggatcgtcc atgacagtaa aaacggaatc atcggcagct gcattcagtg acgcaaacat
      961 gccccccgcc aacataacga tggcaggaac tgtcttcaaa agcttcattt atcaagagtc
     1021 cgtacaacaa aaaaagagac catcgcggtc ccggaaactt tcttaaggat caaagattag
     1081 cgtccctgga aaggtaacga atta
//
LOCUS       DAZOYL010000532         1103 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZOYL010000532 DAZOYL010000000
VERSION     DAZOYL010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.536"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(95..596)
                     /gene="aqpZ"
                     /locus_tag="ACHUOY_004267"
                     /pseudo
     CDS             complement(95..596)
                     /gene="aqpZ"
                     /locus_tag="ACHUOY_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728363.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
     gene            complement(715..>1103)
                     /locus_tag="ACHUOY_004268"
     CDS             complement(715..>1103)
                     /locus_tag="ACHUOY_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFR0684833.1"
                     /translation="KIYDLAKRMIRLSGLSVRDDKNPDGDIAIEVTGLRPGEKLYEEL
                     LIGDSVQGTSHPRIMTANEVMLPWQDLSLLLKELDQACHDFDHERIRSLLLQAPAAFN
                     PTDDICDLVWQQKKSLLSQASNVIRL"
BASE COUNT          315 a          279 c          260 g          249 t
ORIGIN      
        1 gatttggctg tcggtaaatt ttgatgtttt cataaagaat ctcctgcgtt tatggtacga
       61 gaaaattcta cttttgacgc tggttaattt acggggtgat taccagacca atcgccagtg
      121 gcgcaaagcc cgcaggagcg cgtttttctg tcgctccgga caaaaaacgc actgaggaca
      181 aactcgctca gcatacaggc gcttaacgca tagccgccag gtgaatggag gccataacca
      241 ttagccgcaa aaccgctggt tgtcgcatca aacccagcct taccgctggc gataccatac
      301 agcactgccg cagcggcaat accgccgata acctgagcga taatgtaagg taacacgcgc
      361 gctgctggga agcgtccacc ggcccacaga cccaaggtca ccgcggggtt aaaatgcgca
      421 ccagagatgt gcccgaccgc ataagccatg gtgactactg tcagaccaaa cgccaacgcc
      481 acgcccaaaa agcctatacc taactgtggg aaagcagcag agatcaaggc gctaccacag
      541 ccacccagaa ccagccagaa agtgccgaaa aattcggcgc taaaaggttt aaacatacac
      601 tgatgtcctg tttgttaaag agcaggcatc agggtaaagg cggtcacgat gtaccgccta
      661 gcggggattt gtaagagagg tgaagaattg gtgtggaagg ttaaacctaa gcaatcacag
      721 gcgaatgaca ttgctcgctt gtgataacag cgattttttc tgctgccaaa ctagatcgca
      781 aatatcatca gttggattga atgccgctgg tgcttgtaac aacaaactgc gaattcgctc
      841 atgatcaaag tcatgacaag cttgatccag ctctttaagt aagagcgata gatcctgcca
      901 cggtagcatc acttcgttgg ccgtcataat tcgtggatga gaggtacctt gaactgaatc
      961 accaatcagt aattcttcat acagtttctc acctggacgt aatcccgtaa cttcaatggc
     1021 aatatcgcca tctggatttt tatcatcccg tacactcaag ccacttaacc ggatcatgcg
     1081 tttggctaag tcataaatct tga
//
LOCUS       DAZOYL010000533         1101 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZOYL010000533 DAZOYL010000000
VERSION     DAZOYL010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.537"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(313..936)
                     /locus_tag="ACHUOY_004269"
     CDS             complement(313..936)
                     /locus_tag="ACHUOY_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR0684834.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          285 a          235 c          229 g          352 t
ORIGIN      
        1 ataaatattt attcgtggta atcagagaga gtcgcgacat ttgtgatgtt attcttacaa
       61 ctgtaagaag cgccggtgat ttacaagtaa gagtatgatt acgcttttta aagcgtacca
      121 taaagcataa cataataaat gatttgtgat gacactggat tattgcggtt tactttgagg
      181 aaaataaaat gcgtgtttat agaaccgcga gcgccaaata attttatgac cacaacctgt
      241 cgtattgtgg tcataaaaaa ttgtcggctg gagggagtta aatcctttac ctcaatatac
      301 tgcctgtcag cattagttca caaacagtgc ttcgctatcg acgatgattc catcttccgc
      361 acctttaagc gccaggtttg cctcgtagta ttgcttttta tcaagcagtt tctgcgcatc
      421 agcaagtgca ttacgagtct gtttaagcgg catcagatat tggttttcca ttacgccaac
      481 accggccagg cgaagctctt ccattgcgcc ctttttatcc cctttcgcca ttttttcatt
      541 ggcaattttt atcgcggctt ctttttcggg tgttgctaca taactctcag aaattccgac
      601 agatgcatta atgacaatat attggtcatc attgagattg gtctttttac ctggcttagc
      661 gaactttgcc cattcggtac tgtcatctga cagcaaagcg gaagcttcat tggtcagttc
      721 ttttgccttt tccggatcgc catgaaacag agccaggcgc gctacctgca cgtcacgcat
      781 cgcatataaa ccttgttcag agatacgctc tgctgctgcc gttttctggg tttgctgagt
      841 ttgctgttgt gccgctaccg gtgcattatc agcagcccat acagttgaag atgccagaat
      901 tgctgtgacc atcgtagcca taattaaacg tttcatactt acctcattct ttaacagttt
      961 tagaaaacgc cgccatcata ttgggtgagc atgaaatgag aaatcagaga gcaacggtca
     1021 ttttgaaagg gttatcttac aattgtagtt attatcgcaa agcggcattt agtcttacag
     1081 ttgtaggaat atgcttcctg t
//
LOCUS       DAZOYL010000534         1096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZOYL010000534 DAZOYL010000000
VERSION     DAZOYL010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.538"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..500)
                     /locus_tag="ACHUOY_004270"
                     /pseudo
     CDS             complement(1..500)
                     /locus_tag="ACHUOY_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498202.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
     gene            443..837
                     /locus_tag="ACHUOY_004271"
                     /pseudo
     CDS             443..837
                     /locus_tag="ACHUOY_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          233 a          312 c          245 g          306 t
ORIGIN      
        1 atcgtcagcg tacctctcaa acggtacccc cgatattctc tttccatcca caaatcaaat
       61 gcatagtgat ggaacaagtt agcaagtagc ggactaatga ccccgccctg cggagtgcct
      121 cgtgttcggg ttattaattc tccattttca caactctgca ttggcgcttc catccagcgt
      181 cgacagtaca ggatgaccca tttaggcatc ccgtggtgtt ccagagcttt gagcaccaga
      241 tcatgtctca catggtcgaa aaacgcactg atgtccactt ccagtatcca gctataacgc
      301 cagcaccgga tggcacattg tttcagagcg tcatgagctg atttgcctgg tcggtaacca
      361 tatgaatcag catggaaaat cgggtcaagc ttttcttcca tgaaaagttt tatcgctccc
      421 tgagcaattc gatcggatac tgttgggatc cccagaatgc gttccttgcc attcgacttc
      481 gggatccgtt tttccagcac tggtggcgga aaccatgttc ccgaacatag ccggttccag
      541 atcttgtata gattgccatc acgttgctgg tcgaacattt tcaaagtctg cccgtcgcat
      601 ccgggggctc ccttgttgcg acgcacatcc agatatgatg cccagaccag tgcttttggt
      661 atttcaaatg attttcgttg catcagttac gtcatcccct tttgggttgt gtcttctgca
      721 atgaatgtga tccccatccc ttcgctccag acccattaca gatctttcat cactactacg
      781 aatgggtccg cccctgtttc ccgatacgtg ccggaaacag gttctcacgt tccctgagat
      841 ggtctggccc gggatcctgc tgccttcaca ccggatgccg cgcagtcagt aagcaggtgt
      901 cttctgcgct tatcaggggg cggctgtacg gccccatttt gacatcatct aagatttcga
      961 tgcttcatcg gacagtttac tttcgttcag cttcccgaac cccacgtgac agtagatctc
     1021 tgccttttcc cgagtcgctc accacctcat tccttagaat gaagcagcac cgggtcgttt
     1081 ggtcagtgct cctgta
//
LOCUS       DAZOYL010000535         1096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZOYL010000535 DAZOYL010000000
VERSION     DAZOYL010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.539"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..1074
                     /locus_tag="ACHUOY_004272"
     CDS             join(<1..167,167..1074)
                     /locus_tag="ACHUOY_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFR0684835.1"
                     /translation="EGASRLSLPEGTLGQWVTAARKGLGTPGSRTVAELESEILQLRK
                     ALNEARLERDIFKKSNSVFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQ
                     HEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRL
                     RCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIK
                     DVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQE
                     QSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQ
                     RRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          312 a          261 c          286 g          237 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtctggtctg
      841 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      901 ggaacgctga aaaatgagag cctgagccac tatcgtttta ataaccggga tgaagccatc
      961 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
     1021 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
     1081 aacaaatggt agtgtc
//
LOCUS       DAZOYL010000536         1092 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZOYL010000536 DAZOYL010000000
VERSION     DAZOYL010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.540"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>1092)
                     /locus_tag="ACHUOY_004273"
     CDS             complement(<1..>1092)
                     /locus_tag="ACHUOY_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR0684836.1"
                     /translation="FFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGRDR
                     ITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEKAS
                     DLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESLLT
                     SLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYCLW
                     LYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALDIF
                     LSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGLAD
                     DAEWLFYNASFGMQHPILHAIMYCKTDNC"
BASE COUNT          328 a          231 c          197 g          336 t
ORIGIN      
        1 taacaattat cagtcttaca gtacattatc gcgtgaagta tgggatgctg catgccaaaa
       61 ctagcattat agaatagcca ctcagcatca tcggccagac caatattagt ggtcagtttt
      121 attaacataa cataagtgct aaatagttca tgaacagaac aatattcata gcctgtataa
      181 gtggcattag ctgtgcgtcc tgtaatccac tcatcaccaa tgggagcgcc agatggtgta
      241 agataatatt tcaactggga taaaaaaata tctaatgcag tttgatactg cggccgttgg
      301 ctcgctgcga tggctaatgc cctaatatgc tcatacgtat gtacgccatg acctttaaga
      361 aggtaaccag ggtctatgac atttttatat tgcagatctt gctccgacac gcatcctgcg
      421 gaataatctt catacagcca caagcaatat gagagatatt caatgttatt ggttatttca
      481 taaaggcggt agaaaacatc tgttattgtt aatccgtggc tatgtccagc aaaaccctgt
      541 tccacaacga aaggatgtga cgtttcttta gggtagccag ccataatgca ttgtgcggct
      601 tgggtaagag aagttaataa actctcatta cgggtggact cataacatcc aagcacaaaa
      661 cgcaaggcag tagcttgtgc ccagaactct ccgttttctg ctttacagtt aaattttaat
      721 gatgaatcat aaattccaat gtagccatca tcatcttgcg tatcgataag ttccaaacat
      781 aaatcgctcg ctttttcctg ccattcttta ttatctaaca agtaggcact gcggcaatat
      841 ccatccttcc aattcgattg agtttcacta tcccaccaat aatattgcat catgttatca
      901 tcgggattat tctgtttttt aataacgcca tcttcagcag atacagtgcg tggagttata
      961 cgatctcttc catatatttt atgctccgta ataagatggg gaactaattt atccagttct
     1021 cctatatgac cgttcatata ccattccatc ttattcttta accaaccagt gggctttata
     1081 tcgttaaaaa aa
//
LOCUS       DAZOYL010000537         1088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZOYL010000537 DAZOYL010000000
VERSION     DAZOYL010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.541"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            212..>1088
                     /gene="nadA"
                     /locus_tag="ACHUOY_004274"
     CDS             212..>1088
                     /gene="nadA"
                     /locus_tag="ACHUOY_004274"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0684837.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          294 a          259 c          265 g          270 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgca
//
LOCUS       DAZOYL010000538         1073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZOYL010000538 DAZOYL010000000
VERSION     DAZOYL010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.542"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            180..965
                     /locus_tag="ACHUOY_004275"
     CDS             180..965
                     /locus_tag="ACHUOY_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684838.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          354 a          186 c          225 g          308 t
ORIGIN      
        1 tcatactatc aaatcattca atttaaatat atttgaatgt tatgattttc atttttctat
       61 tgatttaatg aatttatata tactgttatt tttgtttgtt ctgattcaag tggagtatat
      121 aattaaaaat cgttagctaa acattaccat gattatgaca ggcgagggag tgtccgatta
      181 tgaatttaag aagggtttgt tgtatatgtg tattattctc gctgcttgca ggatgtgtct
      241 ctgaatcttc tattgatgaa aagaagaaaa aagctcaagt cacacaaagt aatattaata
      301 aaaacactcc ccagcaactg acagacaaag atttattcgg taatgaaacc actctggccg
      361 tatccgaaga ggatattcaa gctgcgcttg atggagatga gttccgcgtt ccccttaatt
      421 ctcctgtaat acttgttcaa tccggcaacc gcgcaccgga aaccattatg caggaggaga
      481 tgcgaaaata ttatactgtt tccacattct ctggtatccc ggacaggcaa aagcctctga
      541 cttgtaacaa aaacaaagat aaaaatgaga acgaggatgt tgccagtgct gagaatatga
      601 actggatgca ggcactgcgt tttgtggctg caaaagggca tcagaaagcg attattgttt
      661 tccaggatac gttgcagaca ggaaaatatg actctggatt gaaatcaata gtttggtcag
      721 attataaaaa tcaaaaattg actgacacga tgtcactgcg ctatctggta cgattcacgc
      781 tggtggatgt agctacaggt gagtgggctg cctggtcgcc ggtgaattat gaatataaag
      841 tgcttccacc attgcccgac aagaatgaag ccagtacgac tgatatgaca gagcagcaaa
      901 tcatgcaact taagcaaaag acctataaag cgatggtaaa agatttggtt aaccgctatc
      961 aataataaat tatatctgcc gccaggaatt tctggcggaa ataatacaaa attctttgca
     1021 taagaaattt ttcttgacaa agacagtgca agaaaggaca tggaatccgg agt
//
LOCUS       DAZOYL010000539         1045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZOYL010000539 DAZOYL010000000
VERSION     DAZOYL010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.543"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..875)
                     /gene="rhtA"
                     /locus_tag="ACHUOY_004276"
     CDS             complement(<1..875)
                     /gene="rhtA"
                     /locus_tag="ACHUOY_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR0684839.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKE"
BASE COUNT          302 a          284 c          258 g          201 t
ORIGIN      
        1 aattctttta ttttgctctc tttgcgtact gtcagcgtag accccattga agcggcgatg
       61 atagcgccga gcgccagcag ctgtatgggt gtcagtgttt ctccgaggaa aatcatcccg
      121 gaaacggcag ccagcgccgg ttccatgctc atcagcgtac caaatgtccg tgttggcaaa
      181 cgggtgaggg caatcatttc cagcgaataa ggcagagcgg tcgagagaat agcgacagcc
      241 agacccaatg gaataaccga ccagtgccag agtgcttcac cagcctgaag cgctccaatt
      301 ggcacgaaaa ttaacgctgc aatcaacgaa ccaattgcca ccgtcgcagg gccatgttcc
      361 gctcctgcgc gttgcccact taaaatgtaa atagcccaac aagccccggc ccccagtgcc
      421 agcgcacagc cggttaaatc gacatgggaa acgtcttgcc ccagcggtag caggaaccac
      481 agaccaagaa ccgccagcac aacccagacg aaatctaccg ggcgacgaga agagaacagc
      541 gccaccgcca gtggtccggt gaactccagc gccaccgcaa tacccagcgg tactgtctga
      601 atagaaagat aaaaaagata attcatccca cccagcgaaa cgccgtaaaa caacagcggt
      661 aaccgttgct ctttggcaaa gcgcagtcgc catggcttaa agaacgcgat gaggatcagc
      721 gttcctaatg ccagacgcag cgcagtgaca cccggtgcgc ccaccagagg aaaaagtgac
      781 ttagctaacg aggctccacc ctgaatagac gccatggcaa cgagcaatat gactattggt
      841 aaccagaccg gcattttacg taatgaacca ggcatccttt ctcccacaaa tatctagact
      901 taagtaaagc gtggagtgta ctggatatac ccaatgctgg ttgagcattt gttgaaaaaa
      961 ttttcccccg ttttgactaa aatgcgccag gattgatgga atcattagtc tggtgattag
     1021 gaataatctg gatgaatgac aggga
//
LOCUS       DAZOYL010000540         1034 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZOYL010000540 DAZOYL010000000
VERSION     DAZOYL010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.544"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>1034
                     /locus_tag="ACHUOY_004277"
     CDS             <1..>1034
                     /locus_tag="ACHUOY_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFR0684840.1"
                     /translation="DEIWKAMLTLQFHDILPGSCISRVYHETEKEYLKLEAKTEKIIS
                     DAQSTLLSKIDTSSYKDPHILFNTTCFARNEWININNNWLKARVNSYGYAVIVPKNKI
                     VNGLKAESQSIENNYIKLLFSESGDLISLYDKRYGKEYITENMHSEIRAYHEDAGFFA
                     AWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIYHYNSSYLRLAFTLTQDSPRVDV
                     QTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDRPTHSDDSFAFAKDEIPAHHWV
                     DLSDRETGIALIAKNKYGYRVKDQTLELTLLRSQHKPGEVVKTDNYDNFLINNFADIG
                     KQKFIFSLFP"
BASE COUNT          336 a          196 c          187 g          315 t
ORIGIN      
        1 ttgatgaaat atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat
       61 gtatttcaag agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg
      121 agaaaattat ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata
      181 aagatccgca cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa
      241 acaacaactg gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga
      301 ataagatcgt aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac
      361 ttcttttttc agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat
      421 acattactga aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg
      481 cggcctggga ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa
      541 aaatgacaac tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt
      601 cctcttatct tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa
      661 cgtttattga ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac
      721 aaacatctct tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg
      781 atgattcgtt cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg
      841 atcgagaaac tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc
      901 agacgcttga attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag
      961 acaattatga taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat
     1021 tctccttatt tcct
//
LOCUS       DAZOYL010000541         1029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZOYL010000541 DAZOYL010000000
VERSION     DAZOYL010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.545"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            61..1005
                     /gene="leuO"
                     /locus_tag="ACHUOY_004278"
     CDS             61..1005
                     /gene="leuO"
                     /locus_tag="ACHUOY_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR0684841.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          279 a          218 c          250 g          282 t
ORIGIN      
        1 ggattatttc tctgcattcc aataagggaa agggagttaa gtgtgacagt ggagttaagt
       61 atgccagagg tacaaacaga tcattcagag acggcggagt taagcaagcc acagctacgc
      121 atggtcgatc tcaacttatt aaccgttttc gatgccgtga tgcaggagca aaacattacc
      181 cgtgccgctc atgttctggg tatgtcgcaa cctgcggtca gtaacgctgt tgcacgcctg
      241 aaggtgatgt ttaatgacga gctttttgtt cgttatggcc gtggtattca accgactgct
      301 cgcgcatttc aactttttgg ctcagttcgt caggcattgc aactagtaca aaatgaattg
      361 cctggttcag gttttgaacc cgcgagcagt gaacgtgtat ttcatctttg tgtttgcagc
      421 ccgttagaca gcattctgac ctcgcagatt tataatcaca ttgagcagat tgcgccaaat
      481 atacatgtta tgttcaagtc ttcattaaat cagaacactg aacatcagct gcgttatcag
      541 gaaacggagt ttgtgattag ttatgaagac ttccatcgtc ctgaatttac cagcgtgcca
      601 ttatttaaag atgaaatggt gctggtagcc agcaaaaatc atccaacaat taagggcccg
      661 ttactgaaac atgatgttta taacgaacaa catgcggcgg tttcgctcga tcgtttcgcg
      721 tcatttagtc aaccttggta tgacacggta gataagcaag ccagtatcgc gtatcagggc
      781 atggcaatga tgagcgtact tagcgtggtg tcgcaaacgc atttggtcgc tattgcgccg
      841 cgttggctgg ctgaagagtt cgctgaatcc ttagaattac aggtattacc gctgccgtta
      901 aaacaaaata gcagaacctg ttatctctcc tggcatgaag ctgccgggcg cgataaaggc
      961 catcagtgga tggaagagca attagtctca atttgcaaac gctaactgaa tgcagaatag
     1021 gtcagacat
//
LOCUS       DAZOYL010000542         1028 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZOYL010000542 DAZOYL010000000
VERSION     DAZOYL010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.546"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..934
                     /locus_tag="ACHUOY_004279"
     CDS             <1..934
                     /locus_tag="ACHUOY_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0684842.1"
                     /translation="EAGLKRVVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPD
                     VVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNLLNEENPHQLQ
                     QLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMDP
                     LGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSV
                     DLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGK
                     KEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
     gene            936..>1028
                     /locus_tag="ACHUOY_004280"
     CDS             936..>1028
                     /locus_tag="ACHUOY_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFR0684843.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSN"
BASE COUNT          334 a          186 c          262 g          246 t
ORIGIN      
        1 tgaagccggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag
       61 tcagtttgac gccgtgggca ggctcaggac acagacggat gccgcaggcc ggacaacaga
      121 atacagcccg gatgtggtga cgggtctcat cacgcgcatc accacgccgg atggcagggc
      181 atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct
      241 ggaaatacgc cgggaatatg atgaatgggg caacctgctg aatgaagaga acccgcatca
      301 gctgcagcag cttattcgtt tgccggggca gcagtatgat gaggagtccg gcctgtatta
      361 taaccgccac cgctattatg acccgctgca ggggcggtat atcactcagg atccgattgg
      421 gctgaagggg ggatggaacc tgtatacata tccgctgagc ccggtgaata gcatggatcc
      481 attaggatta tatgaattta aatcaaaaaa tattgatgat attggaatat ttgcattggc
      541 aatgtgtaat ggagaatcaa ttaacgagaa taaagaatat ggtggactaa tatgtaagaa
      601 gcaaggtgaa tatttcccca tgaatccgat aagttcaaat gataatgata gtgtagactt
      661 gcgaaatata aaatgccctg aaggttcaga gagagtaggc gattatcaca ctcacggttt
      721 ttactctgac gataaaggaa ataaagtaac aaaagaaaat gatgtttatg atagtctaaa
      781 tttttcaagc aaagatttaa cgaattctta tatgaatgga atgggaaaaa aagaatacag
      841 tagttacttg ggaacaccaa ataacaccta tctaaaatat aatcccaaag ctaaagggaa
      901 tggagttaca attatcaggc aagggagtaa ttaaaatgaa ggcaatacaa tgttgttatt
      961 tccttgcttt agtctccagt catttaatgg ctggagttat gggtgatgat aatatatttt
     1021 taagtaat
//
LOCUS       DAZOYL010000543         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZOYL010000543 DAZOYL010000000
VERSION     DAZOYL010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.547"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHUOY_004281"
     CDS             complement(77..865)
                     /locus_tag="ACHUOY_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0684844.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHUOY_004282"
     CDS             complement(905..>1023)
                     /locus_tag="ACHUOY_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0684845.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZOYL010000544         1015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZOYL010000544 DAZOYL010000000
VERSION     DAZOYL010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.548"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..1015
                     /locus_tag="ACHUOY_004283"
                     /pseudo
     CDS             1..1015
                     /locus_tag="ACHUOY_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          249 a          246 c          309 g          211 t
ORIGIN      
        1 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
       61 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      121 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      181 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      241 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      301 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      361 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gatcatgcgc cagaatgcgc
      421 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      481 ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg
      541 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
      601 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      661 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      721 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      781 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      841 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      901 cagaggcgtt cgagcattat aacgaatggc atccgcatag tgcgctgggt tatcgctcgc
      961 cacgggaata tctgcggcag cgggcttgta atgggttaag tgataacaga tgtct
//
LOCUS       DAZOYL010000545         1013 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZOYL010000545 DAZOYL010000000
VERSION     DAZOYL010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.549"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHUOY_004284"
     CDS             complement(<1..906)
                     /locus_tag="ACHUOY_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR0684846.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          284 a          235 c          204 g          290 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgt
//
LOCUS       DAZOYL010000546         1000 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZOYL010000546 DAZOYL010000000
VERSION     DAZOYL010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.550"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>1000
                     /locus_tag="ACHUOY_004285"
     CDS             <1..>1000
                     /locus_tag="ACHUOY_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFR0684847.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          220 c          253 g          234 t
ORIGIN      
        1 agtggtaacc tgatcgaagg tctcactgtg tactttgcct taaaaagcgg ctctgccaca
       61 ttaacgtcat taacagcggt gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga
      121 gcgatgacag gtagcgtcac ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta
      181 gatataacgc tggtggcagg cccagcagac gcctcaaagt cagtccttaa gaacaatcgg
      241 tcatcattga aaggagactt taccgatagt gctgagctac atcttgttct gcacgatata
      301 tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg tacagtcagg taccaacgtg
      361 ccctatgtgc aagttagtgc aattgactac agtaaaaatt tctcaggcga gtacaaagcc
      421 actgttacag gaggcggaga gggtatcaca acgctgatcc ctgtattgaa tggtgttcat
      481 caagctggtc tgagtaccac aatacaattc actcgcgcag aagacaaaat aatgagcggt
      541 acagtatcag tcaatggtac tgacctaccg acaactacat tcccttcgca ggggttcacc
      601 ggggcgtatt atcagttgaa taatgacaac tttgccccag gaaaaacggc ggctgattat
      661 gagttttcaa gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa
      721 aatgtcggca gcaattcgga aaggattacg gcgacaccaa aatcaggagg ccctagctat
      781 gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg gcgaggcttt catgatatac
      841 agccttgctg aaaatttttg cagcagcaat ggctacacgc tccccagagc aaactattta
      901 aaccacagta gttcccgagg catcgggtca ctgtacagtg aatggggaga tatggggcat
      961 tacacgactg aagctggttt tcaatcaaat atgtattggt
//
LOCUS       DAZOYL010000547          997 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZOYL010000547 DAZOYL010000000
VERSION     DAZOYL010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.552"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            18..842
                     /gene="gadX"
                     /locus_tag="ACHUOY_004286"
     CDS             18..842
                     /gene="gadX"
                     /locus_tag="ACHUOY_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR0684848.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          295 a          187 c          220 g          295 t
ORIGIN      
        1 gtaatattaa gtcaactatg caatcattac atgggaattg tctaattgcg tacgcaagac
       61 ataaatatat tctcaccatg gttaatggtg aatatcgcta ttttaatggc ggtgacctgg
      121 tttttgcgga tgcaagccaa attcgagtag ataagtgtgt tgaaaatttt gtattagtat
      181 caagggatac gctttcatta tttctgccga tgctcaagga ggaggcatta aatcttcatg
      241 cacataaaaa aatttcttca ttactcgttc atcactgtag cagagatatt cctgtttttc
      301 aggaagttgc gcaactatcg cagaataaga atcttcgcta tgcagaaatg ctacgtaaaa
      361 gagcattaat ctttgcgttg ttatctgttt ttcttgagga tgagcacttt ataccgctgc
      421 ttttgaacgt tttgcaaccg aacatgcgaa cacgagtttg tacggttatc aataataata
      481 tcgcccatga gtggacacta gcccgaatcg ccagcgagct gttgatgagt ccaagcctgt
      541 taaagaaaaa attgcgcgaa gaagagacat catattcaca gttgcttact gagtgtagaa
      601 tgcaacgtgc tttgcaactt attgttatac atggcttttc aattaagcga gtcgcagtgt
      661 cctgtggata tcacagcgtg tcgtatttca tttacgtctt tcgaaattat tatgggatga
      721 cgcccacaga gtatcaggag cgatcggcgc agggattgcc gaaccgtgac tcggcggcaa
      781 gtattgttgc gcaaggaaat ttttacggca ctgaccgttc tgcggaagga ataagattat
      841 agaattttac tcagacataa aaaaaacccg gcatagggga ccgggaagag gatagtctgc
      901 cgtctccaga ctaataaacc gttataacac tccctgttgg cacgggaaac tttgtgctct
      961 cagtaagtta aatataactt ttactggaaa taagatc
//
LOCUS       DAZOYL010000548          982 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZOYL010000548 DAZOYL010000000
VERSION     DAZOYL010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.553"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..224
                     /locus_tag="ACHUOY_004287"
     CDS             <1..224
                     /locus_tag="ACHUOY_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0684849.1"
                     /translation="PESIFTLTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECP
                     TNTDNVLNECFNNRCTLQICPYGLKQQSP"
     gene            complement(505..636)
                     /locus_tag="ACHUOY_004288"
     CDS             complement(505..636)
                     /locus_tag="ACHUOY_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR0684850.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(672..950)
                     /locus_tag="ACHUOY_004289"
     CDS             complement(672..950)
                     /locus_tag="ACHUOY_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684851.1"
                     /translation="MNIDEASGCFILRQRIDIVNAARAKAFSRLTVLFCTPDRLSGRD
                     VIILNSDAIQRVCDEFMVANSELFALVQEYNRIARTCGMDELRITHLG"
BASE COUNT          314 a          265 c          166 g          237 t
ORIGIN      
        1 ctcctgaaag tatatttaca ctgactcaca caggcatcat gctcaataac aaagtgctac
       61 ctgtaaccat ttacaacgta gtaccattca ataaaacatt ctggaattca atcaaaaata
      121 gccaggaatg ccctacaaat acagataacg tattgaatga atgctttaat aaccgttgca
      181 ctctgcaaat atgtccttat ggactaaaac aacaaagtcc ataaggagtt tactcacatc
      241 tgacaaaatc aatataaaca gcccctccgg agaggggctg gagagtggcg ctatgtgcca
      301 ttgcatggtg ccgggtgcct cccggtgaat tcagtaccag cacctgaatc cgcgattatc
      361 ccatatacct actcgctgat tgcccctccg cacaggggga ttcaccatgc cagtttcttt
      421 taacaaactc cccgcaaacc agacaacagt caaccgcctg aattgtgaag tatttaaaaa
      481 tttctcccgc taactgatac ccggctaaca gtctggcgtt ttctttttca gcaacgggaa
      541 agcaacaacc accacacccg ccaccagcac accgtcagcc agcactgaca ttatccggct
      601 gctgcaatgc cattcacaaa aacagtaagc aatcactttt taccgtaaca ggtgataatc
      661 cagatatgta tctaccccag atgagtaatc cgaagttcat ccataccaca ggtcctggct
      721 attctgttgt actcctgaac aagagcaaat aattctgaat tagcaaccat gaactcatcg
      781 caaaccctct gtatagcatc actattcaga ataataacgt ctcttcccga aagacgatca
      841 ggagtacaga acaaaactgt caaacggctg aaggcctttg ctcgtgctgc attgactata
      901 tcaatacgct gcctaaggat gaaacacccc gacgcctcat caatattcac tctacccaca
      961 ccatatgaat gataaatatt ta
//
LOCUS       DAZOYL010000549          980 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZOYL010000549 DAZOYL010000000
VERSION     DAZOYL010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.554"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(244..894)
                     /gene="zinT"
                     /locus_tag="ACHUOY_004290"
     CDS             complement(244..894)
                     /gene="zinT"
                     /locus_tag="ACHUOY_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR0684852.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          278 a          182 c          202 g          318 t
ORIGIN      
        1 tagtgcacat tgtaaccagc caatataagc aatggcgtga gcacaggtat ctttctctgt
       61 tggccgtatt gtgtatggaa tccgttattg gttaactcaa tgcgggttag cttattaata
      121 tacagacacg tcatagtgat gagcgtgtct gtagctagct tgcgttcagt ggcgtttgtg
      181 gatggaccag tggcagttat gatgacttac taaagcggca tcgaggcgtt atcatgagaa
      241 tactcaatga gacatcattt cctcgaccac ttcttcgcta ctcaactgat atggataata
      301 cgttggccag ttttccattt catttaataa aaattgctgt gaatcattac ccataaaaat
      361 gtgaaaatga gatgattttc gtggtgcaat tatatgatcg ctaaattgta tatatttagg
      421 ggctttgctt tcaggatctt tacattcgaa taagtaacga acgcctttct tgcctgattt
      481 ataggtgagt attttgtatc catcgtaatc atatttacag gatgttgttt cattatttct
      541 atggaattca acaatgccgt cctcaatgcc aatcatctcg atatctgttg cataaccttt
      601 gtgataataa tctttaattt cagcaaatgt tttaatttta tctgcatccg ctttcttctg
      661 aaagacgggg tcaagtttgc cactttgcag taaaggataa acggattgcc agactccatc
      721 ccagtcactg agcgttcggt tttgtacatt ggcatcatca aaaacaccat tagcagcttt
      781 ttgttcgacc tctgttaagg gtttgccgtg tgagtgatga ccatgcgaaa aggcaggagc
      841 gctaacaata aagacaccta aagcaacagc cagtttgtga agacgaattg ccaaaacagt
      901 ttcctccaga gtcattgtat atgatgtgta atgttatatt gtaacatatg gcaaaatgca
      961 acaactctta ggagatgaga
//
LOCUS       DAZOYL010000550          977 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZOYL010000550 DAZOYL010000000
VERSION     DAZOYL010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.555"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            15..>977
                     /gene="istA"
                     /locus_tag="ACHUOY_004291"
     CDS             15..>977
                     /gene="istA"
                     /locus_tag="ACHUOY_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFR0684853.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFD"
BASE COUNT          233 a          258 c          260 g          226 t
ORIGIN      
        1 attcaggaga gcttatggtc acttttgaga cagttatgga aattaaaatc ctgcacaagc
       61 agggaatgag tagccgggcg attgccagag aactggggct ctcccgcaat accgttaaac
      121 gttatttgca ggcaaaatct gagccgccaa aatatacgcc gcgacctgct gttgcttcac
      181 tcctggatga ataccgggat tatattcgtc aacgcatcgc cgatgctcat ccttacaaaa
      241 tcccggcaac ggtaatcgct cgcgagatca gagaccaggg atatcgtggc ggaatgacca
      301 ttctcagggc attcattcgt tctctctcgg ttcctcagga gcaggagcct gccgttcggt
      361 tcgaaactga acccggacga cagatgcagg ttgactgggg cactatgcgt aatggtcgct
      421 caccgcttca cgtgttcgtt gctgttcccg gatacagccg aatgttgtac atcgaattca
      481 ctgacaatat gcgttatgac acgctggaga cctgccatcg taatgcgttc cgcttctttg
      541 gtggtgtgcc gcgcgaagtg ttgtatgaca atatgaaaac tgtggttctg caacgtgacg
      601 catatcagac cggtcagcac cggttccatc cttcgctgtg gcagttcggc aaggagatgg
      661 gcttctctcc ccgactgtgt cgccccttca gggcacagac taaaggtaag gtggaacgga
      721 tggtgcagta cacccgtaac agtttttaca tcccactaat gactcgcctg cgcccgatgg
      781 ggagcactgt cgatgttgaa acagccaacc gccacggtct gcgctggctg cacgatgtcg
      841 ctaaccaacg aaagcatgaa acaatccagg cccgtccctg tgatcgctgg ctcgaagagc
      901 agcagtccat gctggcactg cctccggaga aaaaagagta tgacgtgcat cctggtgaaa
      961 atctggtgag cttcgac
//
LOCUS       DAZOYL010000551          969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZOYL010000551 DAZOYL010000000
VERSION     DAZOYL010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.556"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..578)
                     /locus_tag="ACHUOY_004292"
                     /pseudo
     CDS             complement(1..578)
                     /locus_tag="ACHUOY_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          238 a          266 c          226 g          239 t
ORIGIN      
        1 atgctcttgc caatagcccg acattcaggc ggtgaaagcg gaacgggaag cgatgcgttg
       61 tacatttcga cacgctgaat cacggcatca agccactgtg agaattcggg ccagccctga
      121 cgaatcgccc tgtaagccca tttgcgcgtc atttcgaaca ggtggcagtt tcgccccatc
      181 ccgtaatgtt tatcgatgct acggcgcgct gaggcgctca aatcgagata atcagccagt
      241 tcatcgaggg tataggcttc ctctcgccac tccatcacct gccattccag gtggaacgga
      301 tttttacaaa tcagtccgct gtagttcaca tcagcacaaa gtttctcaca cagcgaacgt
      361 tcaatcgcag cggcgtattt cagcgcctta accgagctgt ccggcgcggt tctcacggcg
      421 atattgagcg cgtagagcag gtgagcatga ccgttcacag gattttttac ggtgatgttc
      481 ggcgcgggag cgttccggtc gctccagtca atcgctgccc cctcgcgatc aacgtcaaaa
      541 accagccaga actgcgcatg aggctggttc tgccggatct ggacatgatt tcaggggcgt
      601 ttgtttttca gtcgcttaca ctacacccaa cggcaaatcc tgtcgaacct tcccgaaaac
      661 ccgatccatt taccataact cccgttgtac gtgccctctc agacgctctc tgacgcaaaa
      721 aactcaaaaa gacaagaaaa aacagggtaa acataaaaaa tgcgtcagaa tcgtttttag
      781 cgcgttgcat aactatgcac gaaagcgacc cgcgatcggt tttgtgtgtt ctgaataccg
      841 aacgggtcaa aatgacacgt tttctctccc tcaatggagc gttctataca gagatttttg
      901 ttcccctatc cattattctg attaccgggt ttccaagctg aaaacaggac ttttctgcga
      961 ccagttttt
//
LOCUS       DAZOYL010000552          962 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZOYL010000552 DAZOYL010000000
VERSION     DAZOYL010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.557"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..229)
                     /locus_tag="ACHUOY_004293"
     CDS             complement(<1..229)
                     /locus_tag="ACHUOY_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR0684854.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            410..562
                     /locus_tag="ACHUOY_004294"
                     /pseudo
     CDS             410..562
                     /locus_tag="ACHUOY_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(587..>962)
                     /locus_tag="ACHUOY_004295"
     CDS             complement(587..>962)
                     /locus_tag="ACHUOY_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684855.1"
                     /translation="SIRNIFYPSRLERLSRFQFIIYTLTSWSILITLNDINLALSFIL
                     VINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVA
                     WILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          315 a          165 c          186 g          296 t
ORIGIN      
        1 aacttgaggc actaaaagca acattaatag aacctgggcc agggaaatgg tatttaataa
       61 gtctgtaagg atcaactttt gctgagatat cgcttaggcc gagtgagaga aagtcttgtt
      121 ctaccatatc accatattgc atatcatcag cagaaaaatc atcgaataaa ctaatagttt
      181 cgaaaagtgt acagggaata ttaattggcg gaagtaatat gttattcatt tgcatttgcc
      241 ttggttatat ttaagctcaa gatcaattcg agaacaaatg ctagggtaaa aaaatattta
      301 aaaaagaagt acacttcaca ttttttcatt acatctctga agagaacctc gttaatgatt
      361 ggtcttgctc attgttaata acaaaatgtt gaatttaccg attaggaaac tttttcctac
      421 gcggattgcc tccagctatg gcatgaactt tgagtttatg ccagaactga agtggagctt
      481 tggctaccct ggcgcgatta tctttatgat ccttgcgggc ctgacaccgt atctgtactt
      541 taagcgtaag aactggctgt aactatacga tgtcgctgtg agtaagttag tgtgctgaca
      601 gcgacatttt agccgaggga atcgcagata acataattgt tgtgaataat atccatgcca
      661 cgttgagttg gacgcttaat gactcacatc tcaccccttc aattgctgca gagataatag
      721 aaaaaagtgt caaacccaat gaaatcaata caaagtagat tgccttacat ccagtatctc
      781 ttattctttt tgctccaata ttgatattta cataggtatt attaataacc agaataaaac
      841 tcaaagccag gttgatatca ttcaaagtta ttaatattga ccatgatgtt aatgtataga
      901 ttatgaactg aaagcgtgat aaacgctcaa gcctggatgg gtagaatata tttctgatgg
      961 at
//
LOCUS       DAZOYL010000553          958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZOYL010000553 DAZOYL010000000
VERSION     DAZOYL010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.558"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>958
                     /locus_tag="ACHUOY_004296"
     CDS             <1..>958
                     /locus_tag="ACHUOY_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HFR0684856.1"
                     /translation="ILGYQANSNGKVIVEKGGEWLIKNNDSSIEFQIGNQGAGEATIR
                     EGGLITAENTIIGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKE
                     YSLVGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIIT
                     AGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGV
                     GNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLM
                     DATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASG"
BASE COUNT          272 a          172 c          266 g          248 t
ORIGIN      
        1 attttaggat atcaagcgaa cagtaatggt aaggttatcg ttgaaaaggg tggcgagtgg
       61 ctaataaaaa ataatgattc ctcaattgaa tttcaaattg gtaatcaagg agctggggag
      121 gcgactattc gcgagggtgg gctgattacg gctgaaaata cgattatcgg tggcaatgcc
      181 accggtatcg gaaccctgaa tgtgcaggat caagactctg tcatcacggt acgcagactc
      241 tataatgggt atttcggtaa tggcacagtc aatatttcca ataatggtct gattaataac
      301 aaagaatatt cattggtggg cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat
      361 aaagggcatt ggagtttcct cggaacaggc gaagctttcc gctatatcta tatcggtgat
      421 gctggtgacg gtgaacttaa tgtctcgcgt gaaggtaaag tagattcggg aattatcact
      481 gcggggatga aagaaacagg cacaggcaac attactgtta aggataagaa ctccgttatc
      541 actaatctcg gaactaatct tggttatgac ggccacggcg aaatgaatat cagtaatgag
      601 gggcttgttg tcagcaacgg aggaagctca ctcggttatg gagaaaacgg cgtcgggaat
      661 gtcagcatca ccacgggggg gatgtgggag gtcaataaga atgtctatac caccattggt
      721 gttgcgggcg tcggaaacct caatattagc gatggcggta agtttgtatc gcaaaatatt
      781 acttttttgg gcgataaagc aagcggtatc ggcacactga acctgatgga tgcgacatca
      841 tcgttcgata ctgtgggtat caatgtcggt aattttggta gcggtatcgt aaatgtcagt
      901 aatggtgcca cccttaattc aacgggctat ggatttatcg gaggaaatgc ctccggta
//
LOCUS       DAZOYL010000554          948 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZOYL010000554 DAZOYL010000000
VERSION     DAZOYL010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.559"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>948
                     /locus_tag="ACHUOY_004297"
     CDS             <1..>948
                     /locus_tag="ACHUOY_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HFR0684857.1"
                     /translation="IFIRQNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVI
                     DELNQQEKPDIIIAATHMGHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVC
                     MASDNKKQWNYIPGTTCVPDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHP
                     VNLKMRITREDGKTEFSFYTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGD
                     RSKVRFVQTSMGHLILSALTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNT
                     VVSIDLTGKEVADYLATVAQMKPDSGAYPQFLNTSFVVK"
BASE COUNT          296 a          171 c          230 g          251 t
ORIGIN      
        1 aattttcatc cgtcagaatt tgaaaattgc agtcattggc ttgacgacag atgatactgc
       61 gaaaacagga aattctgaat attttactga tattgagttc cgtcaacctg ctgctgaggc
      121 gaggagtgtc attgatgaac ttaatcagca ggaaaagcca gatattatta ttgcggcaac
      181 acatatgggt cactatgaca atggcgagtc aggttcgaat gctcctggtg atgttgaaat
      241 ggctcgcagt ttgccgactg gctcattagc aatgattgtg ggaggacatt cacaagctcc
      301 tgtctgcatg gccagcgata ataaaaaaca gtggaattat attccgggga caacctgtgt
      361 accagataaa caaaatggca tctggattgt acaagcgcat gagtggggaa aatatgtcgg
      421 tcaggctgat tttgaattct gtaacggtac aatgaaactg gttaattatc agttacaccc
      481 agttaatctc aaaatgagaa ttaccagaga agatggaaaa acggaatttt ctttttacac
      541 ccccgaaatt actgaagatc cacagatgtt atccttactg acaccattcc agaataaagg
      601 caaagcacaa ctggatgtga aggttggggt tgtgaacgga cgtctggaag gggacagaag
      661 taaagttcgt tttgttcaga ccagtatggg ccatctgatt ctgagcgctc tgacagaacg
      721 tattgacgca gattttgctg ttgtcagtgg tggggaaatt cgagattcca tagagagtgg
      781 taacatcaca tacaaagata tactgaaagt tcagccattt ggtaatacgg tagtaagtat
      841 tgatttgacg gggaaagaag tagctgacta cctagctaca gtggcacaga tgaaaccgga
      901 ttccggagcc tacccacaat ttttgaatac cagctttgta gtcaaaaa
//
LOCUS       DAZOYL010000555          948 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZOYL010000555 DAZOYL010000000
VERSION     DAZOYL010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.560"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>948
                     /locus_tag="ACHUOY_004298"
     CDS             <1..>948
                     /locus_tag="ACHUOY_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0684858.1"
                     /translation="AGLKRVVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDV
                     VTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIT
                     RYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRYDHDRFGQVTAVH
                     REEGLSQYRAYDSRGQLIAVKDTQGHEMRYEYNAAGDLTAVIAPDGSRNGTQYDAWGK
                     AICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHD
                     LTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWR"
BASE COUNT          234 a          278 c          288 g          148 t
ORIGIN      
        1 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
       61 gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga caacagaata
      121 cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg gcagggcatc
      181 ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg acgggctgga
      241 aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc ctgacggcga
      301 tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa cggaagatgc
      361 caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga ccgtcactga
      421 ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga cggcggtgca
      481 ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt taattgcggt
      541 gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg acctgaccgc
      601 cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg gaaaagccat
      661 ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg gacgggtcat
      721 ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac tcgaccggct
      781 gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc tgaccggcaa
      841 acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg aagcagaccg
      901 cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggta
//
LOCUS       DAZOYL010000556          948 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZOYL010000556 DAZOYL010000000
VERSION     DAZOYL010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.561"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>948
                     /locus_tag="ACHUOY_004299"
     CDS             <1..>948
                     /locus_tag="ACHUOY_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684859.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGINTLMNEGFLTKKDLKDFANNSAGLISKAVNGMVKKGYTDAQIGS
                     RLEDLADDLLLTSKYWQRSAREVQASITQQKAAGQWLTET"
BASE COUNT          325 a          189 c          238 g          196 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaaaatac tagatgccat cggtaaagat
      601 gtaaaggaac gcgctaccaa aagctatgac gaggctgaat atgtagttga taagaaaaca
      661 gggaaaggca tatggaaggg cggtgattct ggtattaaca ctttaatgaa tgaaggattc
      721 ttaactaaaa aggatttgaa agactttgcc aataactctg ctggtttaat ttcaaaagct
      781 gtcaacggaa tggtgaaaaa gggctatacc gacgcacaga tcggtagtcg tctggaagat
      841 ttagcggacg acctcctctt aacttctaaa tattggcaaa gatcagccag agaggtgcag
      901 gcaagcatta cccagcaaaa agccgccgga caatggctaa cagaaaca
//
LOCUS       DAZOYL010000557          945 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZOYL010000557 DAZOYL010000000
VERSION     DAZOYL010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.562"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            79..801
                     /locus_tag="ACHUOY_004300"
     CDS             79..801
                     /locus_tag="ACHUOY_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0684860.1"
                     /translation="MLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRARLLKQYQ
                     THLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLS
                     SDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANE
                     MYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRL
                     AEPFWQDHFLGARRILTEETIL"
BASE COUNT          219 a          250 c          254 g          222 t
ORIGIN      
        1 tacgggccag gatacactgc aacatcaatg catgaccagc cattattgag aatgtctttg
       61 ccgtcgattc cctcttttgt gttgtcggga ttactgttgc tttgcttgcc gttttcttca
      121 tttgcctgcg ccaccacatc acatatctct ttcagctacg ccgcccgcca gcggatgcaa
      181 aaccgtgcgc gtttattaaa acagtaccaa acccatctga aaaaacaggc cagctacatt
      241 gtggaaggca atgcccaaag cagaagggcg ctgcgccagc acaaccggga gcagataaaa
      301 cagcatccag aatggtttcc tgcgccgctc aaggcgagtg acagacgctg gcaggcgctg
      361 gcggaaaaca accacttttt aagcagcgat cacctgcata acatcaccga agtggcgatt
      421 caccgtctgg agcagcagct tggcaagcct tacgtctggg gcggcacgcg gcccgatcaa
      481 ggcttcgatt gtagtgggtt agttttttat gcctacaaca agatcctcga ggcgaagctc
      541 ccgcgtacgg ccaatgagat gtatcactac cgtcgggcaa ccattgtggc gaataacgac
      601 ctgcgccggg gagatttgct gtttttccat atccacagcc gcgagattgc cgaccatatg
      661 ggggtgtatc tgggcgacgg tcagtttatt gagtcaccgc gcaccgggga aaccattcgg
      721 gtaagtcggt tagctgaacc tttctggcag gatcattttt tgggcgcgcg gcggatattg
      781 acggaagaga cgattttgta ggacggataa ggcgtttacg ccgcatccgg cggtggtgcg
      841 cagatgcctg atgcgacgct gacgcgtctt atcatgccta caaatctgtg cgtttttgcg
      901 agccgctttc ctgttattcc atcacccagg gaaagatcat cagaa
//
LOCUS       DAZOYL010000558          936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZOYL010000558 DAZOYL010000000
VERSION     DAZOYL010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.563"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>936
                     /gene="gspA"
                     /locus_tag="ACHUOY_004301"
     CDS             <1..>936
                     /gene="gspA"
                     /locus_tag="ACHUOY_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR0684861.1"
                     /translation="QTWRLCYLLGEAGSGKTWLAQQLQKDKHRRVITLSLVVSWQGKA
                     AWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLIIIENAHLNHRRILDDL
                     QRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEHELKASILEGQNIDQPD
                     LLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFTAKQWRMIYRILGDNRP
                     RKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPVVKQDMTKDIAHVVMRD
                     SEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          233 a          221 c          253 g          229 t
ORIGIN      
        1 tcagacctgg cgtctgtgct atttgttggg tgaggctgga agtggaaaaa cctggctggc
       61 gcagcaactg caaaaagata aacatcgccg tgtgattact ttaagcctcg ttgtttcctg
      121 gcaaggtaag gccgcatgga tcgttaccga cgataacgcg gctgaacagg gctgccgtga
      181 cagcgcctgg acgcgagatg agatggcggg gcaattactg catgcgcttc atcgaactga
      241 tagtcgctgc cctcttatta ttattgaaaa tgctcacctg aatcaccgcc gcatacttga
      301 tgatctccaa cgcgcgatat ctcttattcc tgacggtcaa tttttgctga tcggcagacc
      361 cgatcgaaaa gtcgaacgtg attttaaaaa acaggacatt gaacttgtct cgacaggacg
      421 tctgacggag cacgaactta aggcaagcat ccttgaaggt cagaatattg accaacccga
      481 tctcctttta acctccagag ttctgaaacg gatagcttta ttatgtcggg gcgatcgcag
      541 aaagctggcg cttgccggcg aaacaattcg cttattacag caagccgagc agaccagcgt
      601 tttcactgca aaacaatggc gaatgattta ccgcatattg ggtgataacc gcccccggaa
      661 aatgcagctt gccgtggtaa tgagtggaac cattatcgct attacctgtg gttggctact
      721 gctgtcctct ttcacagctc cgttccccgt tcctgcgtgg ctggtacccg taacgccagt
      781 agttaaacaa gatatgacta aggatattgc tcatgtggtg atgcgcgata gtgaggcgct
      841 aagcgtgtta tacggcgtat gggggtacga ggtcccagct gattcggcct ggtgcgatca
      901 ggcagtaaga gcggggctgg catgtaaaag tgggaa
//
LOCUS       DAZOYL010000559          935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZOYL010000559 DAZOYL010000000
VERSION     DAZOYL010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.564"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..294)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUOY_004302"
     CDS             complement(<1..294)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUOY_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR0684862.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEP"
     gene            complement(342..914)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUOY_004303"
     CDS             complement(342..914)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUOY_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR0684863.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          270 a          218 c          189 g          258 t
ORIGIN      
        1 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
       61 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
      121 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
      181 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
      241 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
      301 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
      361 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
      421 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
      481 ttataaagtt taacctgcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
      541 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
      601 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
      661 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
      721 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
      781 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
      841 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
      901 atattacgct tcataaaaac tccattgata taata
//
LOCUS       DAZOYL010000560          935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZOYL010000560 DAZOYL010000000
VERSION     DAZOYL010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.565"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>935
                     /locus_tag="ACHUOY_004304"
     CDS             <1..>935
                     /locus_tag="ACHUOY_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0684864.1"
                     /translation="LVVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEM"
BASE COUNT          233 a          217 c          255 g          230 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacggt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atgga
//
LOCUS       DAZOYL010000561          933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZOYL010000561 DAZOYL010000000
VERSION     DAZOYL010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.566"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(195..>933)
                     /locus_tag="ACHUOY_004305"
     CDS             complement(195..>933)
                     /locus_tag="ACHUOY_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0684865.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          257 t
ORIGIN      
        1 gcttctccga taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg
       61 ttgaccgagc cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt
      121 atcctgtcta tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta
      181 ctgctggcat ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct
      241 aatataaccg tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc
      301 acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc
      361 attccttttg tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc
      421 ggaataccga caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg
      481 gcaatccacg ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa
      541 agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga
      601 ttaatactta ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta
      661 gccgctggac tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt
      721 gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc
      781 agagaatcca ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac
      841 aaggtgactt tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat
      901 ggtgaaacaa ataaaaaagt gagagtcgga cca
//
LOCUS       DAZOYL010000562          926 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZOYL010000562 DAZOYL010000000
VERSION     DAZOYL010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.567"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..926
                     /gene="yfdF"
                     /locus_tag="ACHUOY_004306"
                     /pseudo
     CDS             1..926
                     /gene="yfdF"
                     /locus_tag="ACHUOY_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YfdF"
BASE COUNT          353 a          162 c          166 g          245 t
ORIGIN      
        1 catctatttc aatcaacaat accagcgcaa cttacccaga atccattaat gaaaataaca
       61 atgatgaagt taatggatta gtacaagagc tcaaaaacct ttttaatggt aaggaaggaa
      121 taagcacctg tattaaacat ctacttgagc ttataaaaaa cgccatacga gtaaacgacg
      181 atccttaaga tttaatatta ataattcctc agttacttat attgatattg gctccaatga
      241 tacagaccat attactattg gtatcgacaa ccaagaacca atagaattac ctgcgaacta
      301 taaagacaaa gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga
      361 tatcaataac aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg
      421 ttttatcttc gataagatat cacacaaact cagacatacg gtaacagaat ttgatgagag
      481 cgggaatagc gaaccaacgg acttatttac ctggtacggt aaagataaaa aaggcaactc
      541 tctcgctatt gtaattaaaa ataaaaacgg aaatgattac ttatctctcg gttactacga
      601 tcaggacgac taccacattc aaagaggaat tcgtattaat ggtgatagtc tcacccaata
      661 ttgtagtgaa aaagccagga atgcttcagc aatgcttcag cgtggtttga aagcagtaaa
      721 gctatcatgg cagaatcatt tgcaacaggt tccgatcatc aggttgtaaa cgagctcaac
      781 ggggaaagac tgagagaacc taacgaagtt tttaaacgtt taggtcgagc aataagatat
      841 aattttcaag tggacgatgc aaaatttaga cgcgacaatg taaaagaaat aatttctaat
      901 ttattcgcta acaaagttga cgttga
//
LOCUS       DAZOYL010000563          923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZOYL010000563 DAZOYL010000000
VERSION     DAZOYL010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.568"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..87
                     /gene="ymgI"
                     /locus_tag="ACHUOY_004307"
     CDS             <1..87
                     /gene="ymgI"
                     /locus_tag="ACHUOY_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR0684866.1"
                     /translation="NNSERSLVKQILIEDEIIVCATYLIPDI"
     gene            89..433
                     /locus_tag="ACHUOY_004308"
     CDS             89..433
                     /locus_tag="ACHUOY_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR0684867.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            437..772
                     /locus_tag="ACHUOY_004309"
     CDS             437..772
                     /locus_tag="ACHUOY_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR0684868.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
BASE COUNT          259 a          194 c          245 g          225 t
ORIGIN      
        1 aataatagcg agcgttcttt agttaagcag atcttaattg aagacgaaat tattgtgtgt
       61 gcaacttact taataccgga catctaagtt gaagaaaaaa atacttgcct tcgggcttat
      121 ctcagcattg ttctgctcca cgccagcgat ggcggatatg aaccgcacca cgaaaggtgc
      181 attgctaggc gcaggcgttg gattacttac tggcaatggt gttaatggcg tactgaaagg
      241 tgcagctgtt ggcgctggtg ttggtgcagt aacagaaaaa ggccgcgacg gtaaaaatgc
      301 acgtaaaggt gcaaaagtgg gtgccgccgt tggtgctgtc actggcgttc tgaccggcaa
      361 tggcctcgaa ggggcgatta aaggtgccgt gattggcggt acgggtggtg caattctggg
      421 taaaatgaaa taaggaatga tgatgaaaaa atttgcttta ctggcgggcc tgtttgtttt
      481 cgcaccaatg acctgggcgc aggactacaa tattaaaaac ggtttgccgt cagaaaccta
      541 tattacttgc gcagaagcca acgaaatggc aaaaactgac agcgcacagg ttgcagaaat
      601 tgttgcggtg atgggtaatg ccagcgttgc cagccgtgat ttaaaaattg agcaatctcc
      661 ggaactgagc gccaaagtgg tagaaaaact gaatcaggtt tgcgctaaag acccgcaaat
      721 gctgttgatc acggcaattg atgacaccat gcgtgctatc ggcaaaaaat aatatttaat
      781 atgttaccgc gtcgatgaat tcgacgcggt attttagcta acggggatca gaacttatat
      841 ttcaggccca ccatcactgc ggtgtcattg tagccattat cacccagctg cacgcccaca
      901 ttaccccaga cactggccgc cgg
//
LOCUS       DAZOYL010000564          908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZOYL010000564 DAZOYL010000000
VERSION     DAZOYL010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.569"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..785
                     /locus_tag="ACHUOY_004310"
     CDS             <1..785
                     /locus_tag="ACHUOY_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR0684869.1"
                     /translation="ETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKM
                     HNKAQKGGETCIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIG
                     ELATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKAL
                     TVNGEWQADEYGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADH
                     YTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            810..>908
                     /locus_tag="ACHUOY_004311"
     CDS             810..>908
                     /locus_tag="ACHUOY_004311"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HFR0684870.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAEE"
BASE COUNT          260 a          221 c          237 g          190 t
ORIGIN      
        1 tcgaaactca tcgccaggaa atggccgaaa cagtatggaa agaattaaaa gcaactgact
       61 ctgcaacgtg ccgtagttgc cattcttttg atgccatgga tattgcctcg caaagtgaat
      121 ctgcgcagaa aatgcataac aaagcacaaa agggcggcga aacctgtatc gattgtcata
      181 aaggcattgc ccattttccg ccagaaataa aaatggatga caacgcggcg catgagctgg
      241 aaagtcaggc cgctacttca gtgactaatg gcgcacatat ttatcctttc aaaacttctc
      301 gcataggcga gctggctacc gtgaatcctg gtaccgatct caccgtcgtt gatgccagtg
      361 gcaaacagcc gatcgttctg ttgcagggtt atcaaatgca gggcagtgaa aacacgctct
      421 acctggcggc aggtcaacgg ctggcgctag ccacattaag tgaagaaggt atcaaggcgc
      481 tcacggtaaa cggggaatgg caggctgacg aatacggcaa tcaatggcgt caggcgtctt
      541 tacagggtgc gcttaccgat cccggattag cggaccgtaa accgctatgg caatacgctg
      601 aaaaacttga cgatacctat tgcgctggtt gtcatgcccc tattgccgcc gaccattaca
      661 ccgtcaatgc gtggccgtcc attgccaaag gaatgggggc acgaaccagc atgagcgaaa
      721 acgaactgga cattttaacg cggtatttcc agtacaacgc caaagatatt accgagaaac
      781 agtgatcctc gttgatcagg gaggaagtta tgacattaac aagacgtgaa tttattaaac
      841 acagcggtat tgccgctgga gcgcaggtgg tgacatcggc agcaccgctc cccgcatggg
      901 cagaagag
//
LOCUS       DAZOYL010000565          901 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZOYL010000565 DAZOYL010000000
VERSION     DAZOYL010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.570"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>901
                     /gene="ospD3"
                     /locus_tag="ACHUOY_004312"
     CDS             <1..>901
                     /gene="ospD3"
                     /locus_tag="ACHUOY_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR0684871.1"
                     /translation="KIKKLTVDNFLDEKHQKCYGLISDGMSIFVDRHTPTSMSSIIRW
                     PNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLLAAKNDDGLSGLLLAL
                     QNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQALQNGHAETIKTYGR
                     LLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHADAIRAYGELILSPPLL
                     NSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAKLNLDKKAELLEAKDS
                     NGLSGLFVALHNGCVETIIAYGK"
BASE COUNT          303 a          164 c          182 g          252 t
ORIGIN      
        1 ctaaaataaa aaaactgact gtagataatt ttcttgatga aaaacatcag aaatgttatg
       61 gtcttatatc cgacggtatg tctatatttg tggacagaca tactccaaca agcatgtcct
      121 ccataatcag atggcctaat aatttacttc accccaaagt tatttatcac gcgatgcgta
      181 tgggattgac tgagctaatc caaaaagtaa caagagtcgt acaactatct gacctttcag
      241 acaatacgtt agaattactt ttggcagcca aaaatgacga tggtttgtca ggattgcttt
      301 tagctttaca aaatgggcat tcagatacaa tcttagcata cggagaactc ctggaaactt
      361 ctggacttaa ccttgataaa acggtagaac tactaactgc ggaaggaatg ggaggacgaa
      421 tatcgggttt atcccaagca cttcaaaatg ggcatgcaga aactatcaaa acatacggaa
      481 ggcttctcaa gaagagagca ataaatatcg aatacaataa gctgaaaaat ttgctgaccg
      541 cttattatta tgatgaagta cacagacaga tacccggact aatgtttgct cttcaaaatg
      601 gacatgcaga tgctatacgc gcatacggtg agctcattct tagcccccct ctcctcaact
      661 cagaggatat tgtaaatttg ctggcctcaa ggagatatga caatgttccc ggacttctgt
      721 tagcattgaa taatggacag gctgatgcaa tcttagctta tggtgatatc ttgaatgagg
      781 caaaacttaa cttggataaa aaagcagagc tgttagaagc gaaagattct aatggtttat
      841 ctggattgtt tgtagccttg cataatggat gtgtagaaac aattattgct tatgggaaaa
      901 t
//
LOCUS       DAZOYL010000566          896 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZOYL010000566 DAZOYL010000000
VERSION     DAZOYL010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.571"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..401
                     /locus_tag="ACHUOY_004313"
     CDS             <1..401
                     /locus_tag="ACHUOY_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0684872.1"
                     /translation="IKEDDFLSLDELNDFLSMNESDLEVSSKKTSKQDREITDVSVDS
                     DHKNTQCPATDDELDPFDDEEDEREKAIKRAEKKKRFELARQKNKVSGLDPFDDSEDD
                     YYLSPKKKQTQDDKKQKIKPKMYFAGGGIK"
     gene            401..691
                     /locus_tag="ACHUOY_004314"
     CDS             401..691
                     /locus_tag="ACHUOY_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684873.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
BASE COUNT          323 a          149 c          185 g          239 t
ORIGIN      
        1 atattaaaga agatgacttt ctttctttgg atgaattaaa tgattttctc tctatgaatg
       61 aatctgacct tgaagtaagc tctaaaaaga cttctaaaca agacagagaa atcactgatg
      121 tgagtgttga tagtgaccat aaaaacacac agtgccccgc cactgatgat gagttggacc
      181 ccttcgatga tgaagaggat gaacgtgaaa aagccattaa gagggcagag aagaaaaaga
      241 gattcgagct tgcaagacaa aagaacaaag tatcgggttt agacccattc gatgacagtg
      301 aagatgatta ttatctttca ccgaaaaaga aacaaactca ggatgacaag aagcaaaaaa
      361 ttaaaccgaa gatgtatttt gccggtggag gaattaagta atgaaagaat atcacggtga
      421 aaagagatac aaagattacc tgttaaagcg ttacagcatt tctcgtgaag gtcatcttat
      481 gaaggatact cacggggaag tttaccggat tagaccaaag aaagaaggga ggggttattt
      541 cttttacgat ggtgtgacag atttaaagat tgacgctttg aggtttgcag tgctgtatca
      601 ttttgatatc tggaattcaa tccaccagct acgattaaaa gacggtgacc ccagcaactt
      661 aaaggccacc aatattatca caaaacgtta gtttgtttta ccatcatcaa tcgtaattgt
      721 gatttcatat ttgaaaagct taatgctgaa attgaatcgt gtacttctgg cgataatcat
      781 ttttaaaagc tgatacacaa gaagcaccag aaacacaaag caacctaagc ttgcgaatct
      841 gtgagattca gcaattgttt caagaaagcc cattaattcc ccttatttat caaagg
//
LOCUS       DAZOYL010000567          887 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZOYL010000567 DAZOYL010000000
VERSION     DAZOYL010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.572"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            59..859
                     /locus_tag="ACHUOY_004315"
                     /pseudo
     CDS             59..859
                     /locus_tag="ACHUOY_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          255 a          171 c          214 g          247 t
ORIGIN      
        1 ttggataacg gcacttattg atatattcat gaagattata atcacaaggg aatacataat
       61 gaaaagaaaa gttctggcaa tgctggtccc agcgttatta gttgctggcg cagcaaatgc
      121 ggctgaaatt tataataaaa atggcaataa actggatctg tacggaaaag ttgatgctcg
      181 tcatactttt tccgataacc ctggcgatga cggcgatgaa actatcatcg agttaggctt
      241 caaaggtgag acccaaatta cagatcagtt aactggttat ggtcaagcac tgaccaaaac
      301 aaaggctagc gacactgaag gttctgataa tacttatgtc aaacttgctt ttgcaggtct
      361 gaagttcggc gagatgggct cctttgacta tggtcgtaac tatggtgtga tttacgacgt
      421 tgaagcctgg acagatatgc ttcctgtatt cggcggcgac tcctatacct ggactgataa
      481 cttcatggca ggccgtgcaa atggggtagc tacttaccgc aatagtgatt tcttcggtct
      541 ggtggaaggt ctgaactttg cattgcagta tcaaggtaac aacgaaggca gcaatgcagg
      601 agaagatcaa gaaggtacta aaaatggtca cgaagacgtt cgcttccaga acggtgatgg
      661 ttttggtctt tccacttcat atgactttga cttcggtttg agcttaggtg ccgcttactc
      721 aaactctgac cgtaccaatt ctcaagtagc tcttggcggg tatcactaca atgaatacag
      781 caaatttgct ggtggtgata cagctgaagc atggacattt ggtgcaaaat acgacgccaa
      841 caatgtttat cttgcaatgg gtgatgctgc caacttactg atttagt
//
LOCUS       DAZOYL010000568          887 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZOYL010000568 DAZOYL010000000
VERSION     DAZOYL010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.573"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>887
                     /locus_tag="ACHUOY_004316"
     CDS             <1..>887
                     /locus_tag="ACHUOY_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0684874.1"
                     /translation="FDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYY
                     NHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATEDATG
                     SRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAV
                     KDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGR
                     VIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYD
                     EADRLTHRTVNGETAERWQ"
BASE COUNT          216 a          263 c          262 g          146 t
ORIGIN      
        1 tttgatgcgg tgggcaggct cagggcacag acggatgccg caggccggac gactgagtac
       61 agcccggatg tggtgacggg actcatcacg cgcatcacca cgccggatgg cagggcatcg
      121 gcgttttact ataaccacca cagccagtta acgtcagcca ccgggcctga cgggctggaa
      181 atgcgccgca aatatgatga atccggccgt ctgattcagg aaactgcccc tgacggcgat
      241 atcatccgct accgttatga taatccacac agtgacttac cctgcgcaac ggaagatgcc
      301 accggcagcc ggaaaaccat gacgtggagc cgttacggtc agttgctgag cttcaccgac
      361 tgttccggtt atgtaacccg ttatgaccat gaccgcttcg ggcagatgac ggcggtgcac
      421 cgcgaggaag ggctgagtca gtaccgcgca tacgacagcc gtggacagtt aattgccgtg
      481 aaagacacgc agggccatga aacgcggtat gaatacaacg ccgccggtga cctgaccacc
      541 gtcattgccc cggacggcag cagaaacggg acacagtacg atgcgtgggg aaaagctatc
      601 tgtaccacgc agggcggtct gacgcgcagt atggaatacg atgctgccgg acgggtcatc
      661 cgcctgacca gtgaaaacgg cagccacacc accttccgtt acgatgtact cgacctgctg
      721 atacaggaaa ccggctttga cggccgcaca cagcgttatc accacgacct gaccggcaaa
      781 cttatccgca gcgaggatga ggggctggtc acccactggc actatgacga agcagaccgc
      841 ctcacgcacc gcaccgtgaa tggcgaaacc gcagagcggt ggcagta
//
LOCUS       DAZOYL010000569          885 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZOYL010000569 DAZOYL010000000
VERSION     DAZOYL010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.574"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            134..>885
                     /gene="ipaH3"
                     /locus_tag="ACHUOY_004317"
     CDS             134..>885
                     /gene="ipaH3"
                     /locus_tag="ACHUOY_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR0684875.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          264 a          218 c          141 g          262 t
ORIGIN      
        1 tcgttttttt acagccaatt ttgtttgtcc ttttataata aaaaagtgct gaagttcatt
       61 tcatggaatg aacttcataa aaactcctac ttatttcttt taacaaagcc atttgtccat
      121 cggctttaac tgaatgtcca tcatgttacc gataaataat aacttttcat tgtcccaaaa
      181 ttctttttat aacactattt ccggtacata tgctgattac ttttcagcat gggataaatg
      241 ggaaaaacaa gcgctccccg gtgaaaatcg gaatgaagcg gtctccctac ttaaagaatg
      301 tctcatcaat cagttcagtg agcttcaact gaatcgttta aatctgtcct cgctacctga
      361 caacttacca cctcaaatca ctgttctgga aattactcag aatgccctaa tatcattacc
      421 agaattgcca gcatcgctgg aataccttga cgcctgtgac aatcacctgt caacacttcc
      481 tgaattaccc gcatctctga aacatcttga tgtagataac aaccaactaa ccatgcttcc
      541 tgaattgcct gcattgctgg aatatattaa tgcagataac aatcagctaa ccatgcttcc
      601 tgaattacct acatcgctgg aagtgctctc agtaagaaat aaccagctga catttctccc
      661 tgagttacct gaatcactgg aagcgctcga tgtaagtact aatcttctgg aaagcctacc
      721 agccgtacct gtaagaaatc atcactcaga ggaaaccgag atatttttcc ggtgccgcga
      781 gaatcgcatc acatacattc cggaaaatat acttagcctt gatccgacct gcactatcat
      841 cctcgaagac aatcctctgt cctcacggat cagggagtct ctgtc
//
LOCUS       DAZOYL010000570          882 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZOYL010000570 DAZOYL010000000
VERSION     DAZOYL010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.575"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..680)
                     /locus_tag="ACHUOY_004318"
     CDS             complement(<1..680)
                     /locus_tag="ACHUOY_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0684876.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          265 a          143 c          183 g          291 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt ac
//
LOCUS       DAZOYL010000571          881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZOYL010000571 DAZOYL010000000
VERSION     DAZOYL010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.576"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>881)
                     /locus_tag="ACHUOY_004319"
     CDS             complement(<1..>881)
                     /locus_tag="ACHUOY_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0684877.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLV
                     LTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTH
                     DPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPE
                     SCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKE
                     HADGSITRSEYDEAGRL"
BASE COUNT          137 a          339 c          251 g          154 t
ORIGIN      
        1 ttcagcctcc ccgcctcatc atactcgctg cgggtgatgc tcccgtcggc atgctccttc
       61 ttcaccaccc gcttcaggcc accctcgcct tccgtgtaca gcacctcccg ccggttcagg
      121 ctgtccgtga tgattacccg gctctctccg tactcaaagc ggtagtccag cccctccggg
      181 ttgacctgct ccgtcacccg accggtatca tcataccggt agcagctctc cggcctcccc
      241 gcataatggt gcgccaccat ccgcccggcg tgctccgcat cataagtaaa cccgcgcacc
      301 tgcatcccgc tgcggtcata caccgcccgc agttcaccac cggccgtgta cgtgtagcgc
      361 gccagcggcg cagtcggctg ttcatccggg tatgccgggt cgtgtgtcag ccataccgcc
      421 tccagccgga taccgttatc ggcaccgtac tctgtaccgg cgggcagcgt gtcggggaag
      481 gaaggagctg agagagggca gggactgtca ggggaagata acgaagcggt gtgcggttta
      541 cgggcctctt ccgcccgctg cgcctgtgtg gtcagcacca ggtgaaaacg gcggcccgcg
      601 ccgtccatca cccccgtcac cgcgcccgcc acgtcaccct ccgctgcccg gtgaaaggcc
      661 agcgtccgcc cgaagccgtc caccacgccc gtcagcaccc ggtatgcggg cggctccggc
      721 ggcagcacct cgtccgcccc cggcacccgc tccggccagc tgagtatcca ccacggcccc
      781 tgaaggctgt ttgtcgccag gtacacatgc ggactcaggc gaacatcttc cggcagcacc
      841 tgccagagcg cgcttagcgg ctgcgaactg tgctgcgccg c
//
LOCUS       DAZOYL010000572          877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZOYL010000572 DAZOYL010000000
VERSION     DAZOYL010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.577"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>877
                     /locus_tag="ACHUOY_004320"
     CDS             <1..>877
                     /locus_tag="ACHUOY_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR0684878.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKN"
BASE COUNT          314 a          176 c          161 g          226 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaatta
//
LOCUS       DAZOYL010000573          875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZOYL010000573 DAZOYL010000000
VERSION     DAZOYL010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.578"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>875
                     /gene="cadA"
                     /locus_tag="ACHUOY_004321"
     CDS             <1..>875
                     /gene="cadA"
                     /locus_tag="ACHUOY_004321"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0684879.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKEL"
BASE COUNT          237 a          220 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgc
//
LOCUS       DAZOYL010000574          870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZOYL010000574 DAZOYL010000000
VERSION     DAZOYL010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.579"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..371
                     /locus_tag="ACHUOY_004322"
     CDS             <1..371
                     /locus_tag="ACHUOY_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0684880.1"
                     /translation="SRQEKLIWLRAQMKLTSLCWALKELAKDIWSRPWSEERRNDWQR
                     WLSLAANSDVPMMKNVAKTIGKRLYGILNAMRHGVSNGNAEALNSKIRLLRIKAKGYR
                     NRERFKLGVMFHYGKLNMAF"
     gene            401..802
                     /locus_tag="ACHUOY_004323"
                     /pseudo
     CDS             401..802
                     /locus_tag="ACHUOY_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          247 a          191 c          255 g          177 t
ORIGIN      
        1 aatccaggca ggaaaagctg atatggctgc gtgcgcagat gaagctgacg agcctgtgct
       61 gggcgctgaa agaactggca aaggatatct ggagcagacc atggagcgag gaaagacgca
      121 acgactggca gagatggctg tcgctggcgg ccaacagtga cgtccccatg atgaagaatg
      181 tggctaaaac gatagggaaa aggttgtacg gtatcctgaa cgccatgcgc catggcgtat
      241 caaacggaaa tgcggaagca ctgaacagca agatcaggct gctgagaata aaagccaagg
      301 gctaccgcaa ccgggagcgc tttaagctgg gagtgatgtt ccactatggg aaactgaata
      361 tggcgttcta agccttccca ccatgatcgg ggaagaccca gatatgaaca ggcacattca
      421 gagttacttt aaagagctta ctgaacgtct gaggtgttag agccatgact gccgcagcat
      481 tgctggcgga aatgcctgaa tcgagcagcc tctcgagacg tgaaatcagc gcacttgtcg
      541 gtgttgctca ggtaaaccgg gactcgggaa ctctgcgcgg tcgacgaacc attttcggtg
      601 attgtgctgg ggaagaacag ctctgtacat ggcggcgctt gaggcacccc ggtttaatct
      661 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      721 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      781 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      841 cccgctccgt ttattaccgg aacgggcgag
//
LOCUS       DAZOYL010000575          866 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZOYL010000575 DAZOYL010000000
VERSION     DAZOYL010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.580"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..773
                     /gene="yeeO"
                     /locus_tag="ACHUOY_004324"
     CDS             <1..773
                     /gene="yeeO"
                     /locus_tag="ACHUOY_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR0684881.1"
                     /translation="AILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVES
                     VLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVGQ
                     IAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALFM
                     PIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMFA
                     DWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
BASE COUNT          177 a          174 c          247 g          268 t
ORIGIN      
        1 ttgcaatttt gtgggtgctg gcgattggtt ttaatcctgc gctaaggatt tcgttaaaga
       61 gctattttaa accgctgaat tttagcatta tctgggaagt catggggatt ggtattcccg
      121 cgagtgtcga atcagtgtta tttaccagtg gtcggttatt aacccaaatg ttcgttgccg
      181 ggatggggac cagtgttatt gccggaaatt ttatcgcgtt ttcaattgcg gctcttatca
      241 acttacccgg aagtgcgctc ggctctgctt ctacgatcat tacaggccga aggttggggg
      301 tagggcagat agcgcaagca gagattcagt tgcggcatgt gttctggctt tccactcttg
      361 gattaacggc catcgcctgg ctaacggctc cctttgccgg ggttatggca tcgttttaca
      421 cccaggatcc acaggttaaa catgtcgttg tgattctgat ttggctaaat gctttattta
      481 tgcctatttg gtccgcctca tgggtgctac ccgctggatt taaaggtgct cgcgatgccc
      541 gttacgccat gtgggtttct atgttgagca tgtggggttg ccgggttgtc gtcggttatg
      601 tgctgggcat catgcttggc tggggtgtgg ttggtgtctg gatgggaatg tttgccgact
      661 gggctgtgcg ggccgtgctg ttttactggc gaatggttac tggacgttgg ctgtggaaat
      721 accctcgatc cgaaccgcaa aagtgtgaaa aaaagccagt tgtgtcggaa taaacgacaa
      781 aatgcagatt atttcagcaa acgatttcaa atttaaaaaa caggctttga cattgtgggt
      841 gggcatcgct aatattcgcc tcgttc
//
LOCUS       DAZOYL010000576          854 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZOYL010000576 DAZOYL010000000
VERSION     DAZOYL010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.581"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(497..>854)
                     /locus_tag="ACHUOY_004325"
     CDS             complement(497..>854)
                     /locus_tag="ACHUOY_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0684882.1"
                     /translation="PEKALISKTRLLSENRRKGRVVQAETLEAAGHVLLLTSLSEDEY
                     SAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKELELAKAWIFANLLAAFLIDDIIQ
                     PSLDFPPRSAGSEKKN"
BASE COUNT          203 a          228 c          236 g          187 t
ORIGIN      
        1 tgcacagggc gcaagggaag ccgcagcctg acgcacacgg ctggcgggag ttggagggtg
       61 aaaccgcgaa atctgagcgg gtgaacgacc ggaaaatcac ggcagacggg aaggagggac
      121 cgggtgaccg cagcggcgaa gcgtaccctt gcatcaacgc cagcccggct aagatggagg
      181 cacggcacag cccgcacacg acgctgaggc tacagagctg aaaacccaca aggccatgcc
      241 ccgcatggcc gggtaaagcg gagccggcgg cgacttcctt tgtgctctgt ccggggaccg
      301 gcaggaaaaa ggcggggatt taccccataa gcgctaactt aagggttgaa ccatctgaag
      361 aacgcgacgc ctcggtgcct cgttaagacg atgcctcgcg ttcttcaatt gcgttttgta
      421 ggctgtcagg gatactgtcc cacgaatggc cacctgtaag ctccagatga ccatttttgt
      481 tattctccac aacgcgttag ttcttctttt cggatccggc acttctgggg ggaaaatcca
      541 gcgatggctg gattatgtcg tcaattaaaa atgcggcgag tagattagca aatatccacg
      601 ctttcgcgag ttcaagttcc tttgcacgca aagcatccaa gtgcagcaaa cttttgagcc
      661 gcttaaaagc cagttcaatt tgccatcgca gacggtaaca atcagccact tgctctgctg
      721 aatattcatc ttccgataat gatgttagca atagcacatg gcccgctgct tccagcgttt
      781 ccgcctgaac tactcgtcct tttcgacgat tctcgctgag cagtcgggtt ttactgatta
      841 atgctttttc ggga
//
LOCUS       DAZOYL010000577          852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZOYL010000577 DAZOYL010000000
VERSION     DAZOYL010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.582"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            329..514
                     /locus_tag="ACHUOY_004326"
                     /pseudo
     CDS             329..514
                     /locus_tag="ACHUOY_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(614..>852)
                     /locus_tag="ACHUOY_004327"
     CDS             complement(614..>852)
                     /locus_tag="ACHUOY_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFR0684883.1"
                     /translation="LLTKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVR
                     AFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT          242 a          202 c          190 g          218 t
ORIGIN      
        1 cttaaatacc agtagattac cagaaacttc tgtcgattga agacatcacc cgaagtttga
       61 attcattcga atacttttta cgaggttctt ttcgccagtc ctgtaattcc atacttaggt
      121 gttcgtctat gtcagatggg cgtctaagtt accaattctt gtctaatggc tacatacggt
      181 ggtcggttta cgcttaccgc aggacaggtg gtaactgaaa aatagtttgc tgaggcgact
      241 ggaaacagaa gggcaagatc gattccgaca gcagtgatgg tcagctacct catcgataac
      301 tttgcaacag tgcccttctt gaagcatcat gcttcatgaa gaaaaactgg cacgtcatca
      361 acgtaaacag gcgatgtata cccgaatggt agccttcccg gcggtgaaaa tgttcgaaga
      421 gtatgacttc acgttcgcca ccggagcacc gcagaagcag ctccagtcgt tacgctcact
      481 cagcttcata gagcgcaatg aaaacatagt gttacagggg acatctgaca tcacaaatcc
      541 ccgggtgggg atttgtgtat aagagacagc ccatggagga aggggatata tcgcagctct
      601 gacgactcac tccttattta cccgggaaat acttctccag caatgcatac aggatggcag
      661 cggtttcagc atccattatg ccgtcataat ttttctgccg gaaatgaagc tgaaaagcgc
      721 ggacaagcgc cttatatccg tcatccgtct ccacaccaga aatactgtat ccataggtct
      781 tcagccgtga cacgatatca accttatccg gaatgccctg cgattcaaaa aataaatatt
      841 tagtcagtaa tt
//
LOCUS       DAZOYL010000578          850 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZOYL010000578 DAZOYL010000000
VERSION     DAZOYL010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.583"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(91..>850)
                     /gene="dosC"
                     /locus_tag="ACHUOY_004328"
     CDS             complement(91..>850)
                     /gene="dosC"
                     /locus_tag="ACHUOY_004328"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR0684884.1"
                     /translation="SLPLSQADFGLWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQT
                     MRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLP
                     TIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSD
                     YVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSIGAAMFN
                     GHPDYERLIQIADEALYIAKRRGRNRVELWKASL"
BASE COUNT          228 a          194 c          189 g          239 t
ORIGIN      
        1 catattttgt tcaagggcgg ggaaaaaaat gccatcggcg gcattttccg catcggttag
       61 cttcatgatt acctctgcat cctggcgcat ctaaagactg gctttccaga gttcaacacg
      121 gtttctacct cgtcttttgg cgatatacag agcttcatcg gctatttgaa tgaggcgctc
      181 atagtcagga tgaccattaa acatggcggc accgatggaa agtgagaggg caatatcctc
      241 gccgtttgcg gctttcagtt tggttttctc cacccgactg cgaatacgtt ctgcggtacg
      301 taacgtttcg ttttcagaag cttcagtcaa aacaatgata aattcatcgc ccccgtagcg
      361 aaaaacataa tcactactgt ggacgttgtc ataaaaggcc tgagagactt tacgcagaat
      421 ttcatcgcca gtgttatggc cccacgtatc gttgatctct ttgaatttat caacgtcaat
      481 aatcagcact gacagcggtg taccggtccg gttggcatgg gcaatttcgc gtttgaagat
      541 agtcggcagg aaacggcggt taagtaattt cgttagtaca tccataccga cttcgtggcg
      601 cgatacttct tcaaacaatt cacgcagcaa ggtaataatc tgcgatacgg tatttcttat
      661 ctgtaataaa aatttcaccc gcagacttct gttattcaaa ttcctggtgt tacgcatggt
      721 ttgattgaaa ataccgtcga aatcctgaat gagacgggag atatggccta cttcggcaat
      781 gccactaaaa taatgtcgac ctttatggtt aaaccacagg ccaaaatcag cctggcttaa
      841 tggcaaacta
//
LOCUS       DAZOYL010000579          835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZOYL010000579 DAZOYL010000000
VERSION     DAZOYL010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.584"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..115)
                     /locus_tag="ACHUOY_004329"
     CDS             complement(<1..115)
                     /locus_tag="ACHUOY_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684885.1"
                     /translation="MMTMLLFLHDKVIIMLGTSFNNFGISLSHKRYFSGKVD"
     gene            complement(356..670)
                     /locus_tag="ACHUOY_004330"
     CDS             complement(356..670)
                     /locus_tag="ACHUOY_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684886.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(748..835)
                     /locus_tag="ACHUOY_004331"
                     /pseudo
     CDS             complement(748..835)
                     /locus_tag="ACHUOY_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          238 a          182 c          178 g          237 t
ORIGIN      
        1 catccacctt tcctgaaaaa tatcttttgt gagataaact gattccaaaa ttattaaaag
       61 atgttccaag cattataatc actttgtcat gtaaaaatag taacattgtc attattttag
      121 ctgattcaaa caattctgta aatgctttga gttctttact cccttggctc aatgatggac
      181 cgtctggaat cccagagact aaactctctg agaaagagag tatgaaaaaa cttggggcag
      241 tccctaaatg aagggaccaa cagcggtaaa ataaccggca tcattcataa tgatgccctg
      301 ttagttgtag ttatgaagat ttacgtcatt aaaattgctg ttcgtagtgt cagtcctatg
      361 gtctggcatc agctgaggat cgtagccgat acatcactgg cttcacgaca ttcgtgttga
      421 ggctattcat gaaaactcca cgctgaaaac gccgttttgt ataaacggtc acggtatgcc
      481 cggagccact gttgctgatg aattcgataa aaccttggcg tttcttgaag ctatcgttaa
      541 tgcggataat gaaacaacga ttggtgagat tcgctctttt gccgacgccc tggacgctgt
      601 ccggttcaat cgcaacaaaa tcaaccggca gctgagcaaa cccaaccttg cctctctggc
      661 gctggagcat gaagtgattt ggctgggacg tagccgctga cagcttctat aaaagtatga
      721 tcctacccac gtaatatgaa cacaggctta agtgaggttc tggttttcaa attgttcagg
      781 actgagacct ccacaccaac tgtgccgtcg tcagcgattg taatcgcact tgata
//
LOCUS       DAZOYL010000580          834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZOYL010000580 DAZOYL010000000
VERSION     DAZOYL010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.585"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            8..772
                     /gene="nfeF"
                     /locus_tag="ACHUOY_004332"
                     /gene_synonym="yqjH"
     CDS             8..772
                     /gene="nfeF"
                     /locus_tag="ACHUOY_004332"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR0684887.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT          184 a          223 c          238 g          189 t
ORIGIN      
        1 gataacaatg aataactccc cccgctaccc gcagcgcgtt cgcaatgatc tgcgcttccg
       61 tgaactgact gtgttacgcg ttgagcgcat cagcgccggt tttcagcgca ttgtcctcgg
      121 cggcgaggcg ctggacggtt ttacatcgcg cggctttgac gatcacagca aactcttctt
      181 tcctcaacct gacgctcact ttgtgccgcc aacggtaacg gaagagggca tcgtctggcc
      241 ggaaggacca cgcccaccgt cgcgtgacta tacgccgctg tatgacgaac tacgccatga
      301 actggcgatc gatttcttta ttcacgacgg tggggtcgcc agcggctggg cgatgcaggc
      361 gcaaccgggc gataaactta cggttgcagg tccgcgcggt tcgctggtgg tgccggaaga
      421 ttacgcgtat cagctgtatg tctgcgatga atccggaatg cccgcactgc gccgccgctt
      481 ggaaacgttg agcaaacttg ccgttaaacc gcaagttagt gcgctggtta gcgtgcggga
      541 taacgcctgt caggattatc tcgcgcacct tgatggtttt aatatcgaat ggctggcaca
      601 tgatgagcag gcggtagatg cgcgtctggc gcagatgcaa atccctgccg atgattactt
      661 catctggata accggcgaag gtaaagtcgt taagaattta agccgccgct ttgaagcgga
      721 acagtatgac ccacagcggg tacgtgcagc ggcttactgg cacgcaaagt aactgatata
      781 taaaaagaaa aaggctgacg atttctcgtc agcctttgct atatctggtg gccc
//
LOCUS       DAZOYL010000581          832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZOYL010000581 DAZOYL010000000
VERSION     DAZOYL010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.586"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..610)
                     /gene="yahL"
                     /locus_tag="ACHUOY_004333"
     CDS             complement(<1..610)
                     /gene="yahL"
                     /locus_tag="ACHUOY_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR0684888.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQ"
BASE COUNT          260 a          163 c          154 g          255 t
ORIGIN      
        1 cctgcttaat gggcgcgtct gtctccggaa tagacgacat ttcgcgaagc catttttcca
       61 catgcataca ctctttccct ttagggaaaa gtctcagcaa aggcatatgg tgttcaagaa
      121 gaatacattt gcaatctgtt cgcactgaat tgataaactg cactaatttc ttaagcccgg
      181 taaagcgtcc agcctgcaca tttgctttaa tatcaagaaa cgcatcatcc tggtcatata
      241 cccaaactgt ccctttttga ttttcaagta tgcgaaaaac aaacttaacc gggtgatcag
      301 ggtaatccat ccagtcataa aatcctggga aatcattaaa ctgcacattt atggtgagtg
      361 cgttatcgtc tgaccactca cagtctcgta cggcagccat gagatgaatg agtttgtcgt
      421 catcgactaa atcatcacga caatcataag gataatcata atattgttta atcaaaacct
      481 tgaatatatc gacacaggaa actagggagc ttttgatatc ctctggtaac tgattgtttt
      541 taaccgtatt aagtatgctt tgttgcgtat atggcatcaa attattgtgc ggagctttta
      601 atgatatcat cgcgatctct cttaaataaa aataaacgtc gcagataata aagcgctgat
      661 taaaccaggc actatcagaa atcgctcata actaacgtga gaagtattgt gtactggagg
      721 gagttaattt aacaatttga atttacgtta taggttttac tgctactttg atattatttc
      781 gctctcctta actaaaaagt taagtggaga gcgaagttaa tatgttaaac ca
//
LOCUS       DAZOYL010000582          828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZOYL010000582 DAZOYL010000000
VERSION     DAZOYL010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.587"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>828
                     /gene="waaH"
                     /locus_tag="ACHUOY_004334"
     CDS             <1..>828
                     /gene="waaH"
                     /locus_tag="ACHUOY_004334"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFR0684889.1"
                     /translation="CMESLITQTWTALEIIIINDGSTDNSVEIAKHYAENYPHVRLLH
                     QANAGASVARNRGIEVATGKYVAFVDADDEVYPTMYETLMTMALEDDLDVAQCNADWC
                     FRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSRRWTHVVWMGVYRRDVIVKNNIKF
                     IAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLHNTSVSRLHRQGNKNLNYQRHYIK
                     ITRLLEKLNRNYADKITIYPEFHQQITYEALRVCHAVRKEPDIITRQRMIAEIFTSG"
BASE COUNT          228 a          174 c          198 g          228 t
ORIGIN      
        1 ttgtatggaa tctttaatta cgcaaacctg gactgctctg gaaatcatta ttattaacga
       61 tggttcaacg gataattctg ttgaaatagc aaagcattac gcagaaaact atccgcacgt
      121 tcgtttgttg catcaggcga atgctggcgc atcggtggcg cgtaatcgtg ggattgaagt
      181 ggcaacgggc aaatatgtcg cttttgtcga tgctgacgat gaagtctatc ccaccatgta
      241 cgaaacgctg atgaccatgg cgttagagga cgacctcgac gtggcgcagt gcaacgctga
      301 ctggtgtttt cgtgaaacgg gagaaacctg gaaatccatc cccagcgatc gccttcgctc
      361 aaccggcgta ttaaccggcc cggactggct gcggatgggg ctttcttcgc gccgttggac
      421 tcacgttgtc tggatggggg tttatcgccg cgatgttatt gttaaaaata acattaaatt
      481 tattgccgga ttacatcatc aggatattgt ctggacaaca gaatttatgt ttaatgcgct
      541 gcgtgcgcga tataccgagc aatcgttata taaatattat ctgcataata cgtcagtgag
      601 tcggttgcat agacaaggga acaaaaacct taattatcaa cgtcactata ttaagattac
      661 tcgtctgcta gagaaattaa atcgaaatta tgccgacaaa attacgattt atccggaatt
      721 tcatcagcaa ataacttacg aagcattgcg tgtttgccat gcggtgcgca aagagccgga
      781 tattattacc cgccaacgga tgattgccga gatatttact tccggtat
//
LOCUS       DAZOYL010000583          826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZOYL010000583 DAZOYL010000000
VERSION     DAZOYL010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.588"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>826
                     /locus_tag="ACHUOY_004335"
     CDS             <1..>826
                     /locus_tag="ACHUOY_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFR0684890.1"
                     /translation="PKAKQFEQEFSNYLGANVQSLAVNSATSGLHLALEAVGVKPGDQ
                     VIVPSYTFTATAEIVRYLGADPVIVDVDRKTFNISVDAIEKAITNETKAIIPVHFAGL
                     ACDMDSILSIAKKYDLKVVEDAAHAFPTTYKGSKIGTLDSDATVFSFYANKTMTTGEG
                     GMVVSKNKDIIERCKVMRLHGISRDAFDRYQSKTPSWFYEVVAPGFKYNMPDICAAIG
                     IHQLRKIDDFQKKRQRMAKIYDDALKELPLELPEWPTNASDIHAWHLYPIRLKTDSA"
BASE COUNT          251 a          152 c          172 g          251 t
ORIGIN      
        1 gtcctaaggc taagcaattt gaacaagaat tttctaatta cctaggagcg aacgttcaat
       61 cattagctgt taactctgct acgtcgggct tacatttggc tcttgaagct gttggcgtaa
      121 agccgggaga ccaagttatt gtcccatcat atacattcac tgctactgcc gaaattgtca
      181 ggtaccttgg tgctgatcct gtaattgttg atgtagatcg taaaacattt aatatatcag
      241 ttgatgccat tgagaaggct attactaatg aaacaaaggc gattattcca gtacacttcg
      301 ctggattagc ttgtgacatg gattcaatct tatcaattgc taaaaaatat gacctaaagg
      361 ttgtcgagga tgccgctcat gcatttccta caacatataa aggaagtaag ataggaacgc
      421 ttgattcaga tgctacggtt tttagcttct acgccaataa aactatgaca accggtgaag
      481 gcggaatggt tgtttcaaaa aataaagata taattgagcg ttgtaaggta atgcgtttac
      541 atggaatcag tcgtgacgct tttgaccggt accagtctaa aactccttct tggttttatg
      601 aggttgtagc tccagggttt aaatacaata tgcctgatat ctgtgcggca atcggtattc
      661 atcaacttag aaagatcgat gattttcaga aaaaacgtca acgaatggca aaaatttacg
      721 atgatgcgtt aaaagaattg ccacttgaat tgcctgaatg gcctactaat gctagtgata
      781 ttcatgcttg gcatctatat cctatccgct taaaaactga ttcggc
//
LOCUS       DAZOYL010000584          822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZOYL010000584 DAZOYL010000000
VERSION     DAZOYL010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.589"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            160..330
                     /locus_tag="ACHUOY_004336"
     CDS             160..330
                     /locus_tag="ACHUOY_004336"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0684891.1"
                     /translation="MTHHLGCEKNQLRSGSNSRNGCLTKIITTGDEPLEIRTLRDRNG
                     TFEPQQLKKNQP"
     gene            409..546
                     /locus_tag="ACHUOY_004337"
                     /pseudo
     CDS             409..546
                     /locus_tag="ACHUOY_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          255 a          159 c          189 g          219 t
ORIGIN      
        1 taatttgata tcaaatgaag gtgaatttat gaatgaaaaa cagttgacat ctaataagtt
       61 gcgggcttta gctaatgaac tagccaaaag tctcaaaaat cctgaagatc tcagtcagtt
      121 tgattggatg ctgaaaatga aaccgtactc aatgctaata tgactcatca tcttgggtgc
      181 gagaaaaatc agcttagatc tggcagcaat tcccgcaatg gttgcttaac gaagattatc
      241 actactggag atgaacctct ggaaatccgt actctgcgtg atcgtaatgg cactttcgaa
      301 ccgcaacagc tgaagaaaaa ccagccctag attactaaat ctgtcgggcg atggattaac
      361 tggttcaggc tgtctgatgc tgaaggcctg aagtcactgc tgtcagtgat gtggccggct
      421 cttcatgaga caataacccg taatcatcag tgccgttcaa tatggccact gttgaaaaaa
      481 gttcgtcact ttatggagac ggttagccca cttcccggag aaaaacatag tctggccaaa
      541 gtgtagcggt attaggctca catatttaga tgtagataag ccggagctgg cagttaaatc
      601 taggcagcat cacagaaatg gacaacgccc cttaaggact ggcgtatggc aatgagctgc
      661 tttattatca agtttggtga tcgccttgac ggtcacttct gagtgaaggc gcttgcacag
      721 aatggtaaac ggactcgatt attgaatctt agggataaga tatatctacg atatgtcaat
      781 atacgaaatc gaaagaattt attggtgaat atagccattc aa
//
LOCUS       DAZOYL010000585          810 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZOYL010000585 DAZOYL010000000
VERSION     DAZOYL010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.590"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..634
                     /locus_tag="ACHUOY_004338"
     CDS             <1..634
                     /locus_tag="ACHUOY_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0684892.1"
                     /translation="DTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGG
                     INITNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANS
                     ESETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENN
                     IGVVGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            647..>810
                     /locus_tag="ACHUOY_004339"
     CDS             647..>810
                     /locus_tag="ACHUOY_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0684893.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGN"
BASE COUNT          254 a          160 c          170 g          226 t
ORIGIN      
        1 tgatacctcg cttggtacga atttaagctg gagaactccc atcgccataa tagatggcag
       61 ctatagtcat tctaaaaacg cctggcaaag tggtggaagt attagtagtg gattagttgt
      121 ctggcccggt ggtattaata tcactaacca gttatccgat acatttgcaa ttctggatgc
      181 gcctgggtta gaaggtgcac atattaatgg acaaaaatac aatcgaacaa acagcaaagg
      241 ccaggttgtt tacgacctga taatacctca tcgtgaaaac catctggtac ttgatatagc
      301 aaacagtgaa agtgaaacag aattgcaggg caatcgtcaa attattgcac cttaccgtgg
      361 agcagtttct tatgtgcagt ttacaactga ccaacgtaag ccctggtata tacaggcact
      421 gcgtcccgat ggttcgccat taacctttgg ctatgacgta ctggatctcc aggaaaataa
      481 tattggagtc gttggccagg gtagtcgcct ttttattcgc gtagatgaaa ttcccactgg
      541 aataaaagtt gctctcaatg atgaacagaa cttattctgt actattactt ttcaacacgt
      601 tatcgatgaa aacaaaacat atatatgcca gtaaaaggag tttactatga agtggctttt
      661 attaatcacc ttgagtctat attcttttat tgtgcaaagt gctccctgtg cattgaccaa
      721 tgtcggggaa caacgtggaa catatatact caagccactg agtatgaaag gtaatctcac
      781 cgccgaaaaa attgaaaaca ccggaaatat
//
LOCUS       DAZOYL010000586          806 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZOYL010000586 DAZOYL010000000
VERSION     DAZOYL010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.591"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..806
                     /locus_tag="ACHUOY_004340"
                     /pseudo
     CDS             1..806
                     /locus_tag="ACHUOY_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          310 a          163 c          120 g          213 t
ORIGIN      
        1 ctttacccca ggtataataa atacatatca cggcgatatt tataactgca caacaaatac
       61 cgataatgct aaaacgccag acacaccgaa gtggccttgc gataattggg aagaacaaca
      121 acccattaat tccacattct ctggagaagg atatatctct gatcaatatg atttagtgca
      181 acaccaactt caacaaatta atgcgtgcca cacgaacact acatatacaa atgcagacta
      241 ctcaaaagtt gtggctcaac ttgtaagtct tattactaac attgaaacga taagttcgac
      301 acaacttacg caacagacac aatcaatact taatcagatt aataacatta gatatgaaaa
      361 aaacaaaagt gctgaatgtc gaatcattgt tgtcgctaat cctaaacccg acaaggcaat
      421 cataactaaa attagcgtag aagagggaat acccataaca ttcagtgtcc aaaccatgtt
      481 ttcagatact aattttattg ctgaacaacg agctgactta ccaactaata taaaagatat
      541 tcaaagcctg tatcaaaaaa tgacgaagtt atatattgag cacagtgaaa ataaaaacag
      601 gatgaaagtc tttgccggag ccaactttat tgatttcaat atgacaggac agaacctgtc
      661 cgggttcgtt ctaaccttgt caaggtttta tttcgaagat ctacttaata taaatttcac
      721 tgacgcaaat ttaggtgaca ctatttttta cataaagaac accctacccc caaattatat
      781 aaagatggac aatatcttga caaaca
//
LOCUS       DAZOYL010000587          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZOYL010000587 DAZOYL010000000
VERSION     DAZOYL010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.592"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..376
                     /locus_tag="ACHUOY_004341"
     CDS             <1..376
                     /locus_tag="ACHUOY_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0684894.1"
                     /translation="SYWLRHIYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAV
                     VVFKKEPRKHEITQTVCCGDTLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPI
                     KCIADALRMPLSTSYRIAQRLS"
BASE COUNT          242 a          168 c          153 g          240 t
ORIGIN      
        1 cagctactgg cttaggcaca tttacagcaa taaccactgt catgaaatcc gtattcttca
       61 tcgtgcagtt aaataccttc caccagcagg ccataacggg cttactattc gttcaccgtt
      121 tcctagtgca gtcgttgttt ttaaaaaaga accacgtaag cacgaaatta cacagacggt
      181 ttgctgtggt gacacgctat taccgttaac catcattaac agaggaggct tgagaggaag
      241 acccaccatt tacccacctg agacgctcga cagcttcata aaactataca ggcaaggaaa
      301 acccattaag tgcattgcag atgctctcag aatgcctctg agcacgtcat acaggattgc
      361 tcaaaggttg agctaaaatt ctcataaata tggtcattta caagacaggt gattcagatg
      421 ttaagaagta tccctgtgaa caataaatga ccaatcagta atcattctgt gaaataattg
      481 ttgcaattta taatcgcatc tgttatagtt tctatatggg ctgataaaaa agttcatgtc
      541 ttgaagtggt aacacttggg gaggtgtcag atggttggcg tatattaacc tctcattcag
      601 ggtatacctc agcgttttaa tcatcgtact gtttcaggca tggacttctt tatcagtcca
      661 tcaatccccc agatatttta atcacacagg ctaatcggtt ttatccggtt ggccttttca
      721 cgtatgaggt aatttatgaa tgtctaaata taacccaaat cagttttata aatttgatgt
      781 gcgaatctct caactttcag aag
//
LOCUS       DAZOYL010000588          798 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZOYL010000588 DAZOYL010000000
VERSION     DAZOYL010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.593"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHUOY_004342"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHUOY_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR0684895.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..>798
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHUOY_004343"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHUOY_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR0684896.1"
                     /translation="MKHTPFFFAFFFTFP"
BASE COUNT          257 a          181 c          166 g          194 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggg
//
LOCUS       DAZOYL010000589          796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZOYL010000589 DAZOYL010000000
VERSION     DAZOYL010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.594"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>796
                     /locus_tag="ACHUOY_004344"
     CDS             <1..>796
                     /locus_tag="ACHUOY_004344"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFR0684897.1"
                     /translation="IPGMVDLVEGRAQISNLKKVSIDDLLGRDPVAPDAKLMAENITG
                     KAVMVTGAGGSIGSELCRQIVRYKPAKLVLFELSEYALYAIEKELSALCDKEVLNVPV
                     IPLLGSVQRQNRLQMVMKSFGIQTVYHAAAYKHVPLVEHNVVEGVRNNVFGTLYCAES
                     AIESGVETFVLISTDKAVRPTNTMGTTKRLAELVLQALSARQSQTRFCMVRFGNVLGS
                     SGSVVPLFEKQIAQGGPVTLTHRDIIRYFMTIPEASQLVIQAGAMG"
BASE COUNT          186 a          164 c          218 g          228 t
ORIGIN      
        1 ctattccggg tatggtcgat ttagtcgaag gtcgagcaca aatcagtaat ctaaaaaaag
       61 tatcgattga tgacttacta ggtcgtgatc cggttgctcc tgatgccaaa ttgatggccg
      121 aaaacattac tggcaaagcc gttatggtca ctggggcggg aggctcgatc ggctctgagc
      181 tttgtcgtca aattgttcga tataagccgg ccaaattggt tctatttgaa ctgtctgaat
      241 atgccctcta cgctattgag aaagagctct cggcgctgtg tgacaaagaa gttttgaatg
      301 ttccagtgat ccctctgttg ggctcggtgc agcgtcagaa tcgcttacag atggtgatga
      361 agtcctttgg tattcaaacg gtttatcatg cggccgctta taaacatgtg cctctggttg
      421 agcataatgt ggtggaaggg gtacgtaata acgtgtttgg taccttgtac tgcgctgagt
      481 cagcgatcga aagtggcgtt gaaacttttg tgttgatttc caccgataaa gcggtgcgcc
      541 cgaccaacac tatggggaca actaagcgtc tggccgaatt ggtattgcag gctttgtctg
      601 cacggcaaag ccaaactcgc ttttgtatgg tgcgatttgg taatgtactc ggttcttcgg
      661 gctctgtcgt gccgttgttt gaaaaacaga ttgcccaagg tgggccagtt accttgactc
      721 atcgtgacat tattcgctat ttcatgacaa ttccggaagc atcacagttg gtgattcaag
      781 cgggggcgat ggggca
//
LOCUS       DAZOYL010000590          790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZOYL010000590 DAZOYL010000000
VERSION     DAZOYL010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.595"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            52..790
                     /locus_tag="ACHUOY_004345"
                     /pseudo
     CDS             52..790
                     /locus_tag="ACHUOY_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAZOYL010000591          788 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZOYL010000591 DAZOYL010000000
VERSION     DAZOYL010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.596"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            211..>788
                     /locus_tag="ACHUOY_004346"
     CDS             211..>788
                     /locus_tag="ACHUOY_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFR0684898.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT          172 a          237 c          205 g          174 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcacc
//
LOCUS       DAZOYL010000592          785 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZOYL010000592 DAZOYL010000000
VERSION     DAZOYL010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.597"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..742)
                     /locus_tag="ACHUOY_004347"
     CDS             complement(<1..742)
                     /locus_tag="ACHUOY_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR0684899.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          263 a          137 c          159 g          226 t
ORIGIN      
        1 cacataaaca ggtaaagacc ataaatatgg tgaaagtaaa aaagaaggaa aatacagcaa
       61 tggaaaaaat tttatcggag aacaggaata aatctaaaag atagattgct gaaatacaga
      121 ctccactttt acatgcgcga caaaaattac tgccttgatg aaaaaaccag aaagggaaga
      181 acatcactgc aaatacccaa aaaatagcaa agtaataatg atcactggaa gacgctgatt
      241 cactttctga aggattagtc ggatattggt gttgtgaagt gagttggtta ttcaaagcgt
      301 ttatagctag ctctaatccc tgagctaatt tttgttgttt aaatgcgggt atcatgttgc
      361 tacggatgat atctccagcc agagcatcgg ttaccttttc ttccagccca taacctacct
      421 ggatgcggac agtacgatct gaccaggcaa caatgatcag tatcccatca ttacgtttgg
      481 catctcctaa acgccaattg tcaaaaactc ttgtagcata ttgttcaatt gtctcgtctt
      541 tggttgtggg aacaattaat acagcaagtt gcacttgggt attattgaaa atagattcta
      601 acttgtgcgt cagttctttc ttttcttgtg agtttaagct actcgtaaga tccgtaactt
      661 gttgctgtaa ttgtgggaca atagttggtt gggctgcaac aaaggaacac acgaaagtga
      721 acattagtat gaaatagcgc attataaatt tctcattaaa tctggataaa taaattgacg
      781 gcggc
//
LOCUS       DAZOYL010000593          781 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZOYL010000593 DAZOYL010000000
VERSION     DAZOYL010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.598"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..419
                     /locus_tag="ACHUOY_004348"
                     /pseudo
     CDS             1..419
                     /locus_tag="ACHUOY_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          184 a          218 c          223 g          156 t
ORIGIN      
        1 aaatacggca gtggcgataa cggtactccc ggccgatagt gaatgacctg tttgcctggc
       61 ttgaagagca ggagccgtgc tgtccgcccg acgggccatt aaataaggcg attaactata
      121 ttctgaaccg gcgcgatgaa ctgagctgct tcctgggtga cggagcggtg cccctggaca
      181 acaatatctg cgagcgtgcc attcgcccgg ttgttatggg acggaaagcg tggctgttcg
      241 ccggttcact gatggccgga aaccgggcgg cacagataat gagccttctg taaacagcaa
      301 aacgtaatgg tctggagcca catgcctggc tgacggacgt cctgacgcgt ctgccggaat
      361 ggccggagga gcgactggca gagttgctgc ctcttgaggg atttaccttc accgggtgag
      421 tgatacctgc cgtcaggtgt tcgtgaaccg ggctataacc agcagtcggt tgttgaacgc
      481 ctgacaacag gaaatcagcc aggccagcaa ccacgcggac tgacagagaa tcagaacctg
      541 aatcagcctg aaatcatacc cggggcagta cttcatcacc agtcaccaat acccgtgtca
      601 gtacttcgcg gcctgacgcg atcacccgac tcagtacttc tgagcccgcc tccggtacca
      661 ttctctgtgg gcccgtacac aacaacagaa aagggggccc ggcccggggc gcaaccgtat
      721 tacacttccg gaaaatcgtt atacgcagaa aaatcggatc acgctgtgcc tgctttcatg
      781 t
//
LOCUS       DAZOYL010000594          780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZOYL010000594 DAZOYL010000000
VERSION     DAZOYL010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.599"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>780
                     /locus_tag="ACHUOY_004349"
     CDS             <1..>780
                     /locus_tag="ACHUOY_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0684900.1"
                     /translation="YSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDE
                     IIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHG
                     IAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQA
                     NNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYA
                     PQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQ"
BASE COUNT          240 a          169 c          145 g          226 t
ORIGIN      
        1 tactcattaa acgctcttat taataataaa aggacgccaa tccatctgga cctgcaatgg
       61 gtattaattg ataaccaaac tgcagtttgt ctgactccag agcaattaac attattaggc
      121 tttactgatg agattattga agaggctcag caaaacctga tcgatggttg ttaccctatc
      181 gaaaaagaaa aacaaattac aacttatctc gataaaggga aaatgcaatt atccatatct
      241 gcacctcagg catggttaaa atacaaagat gcaaactgga cgcctcctga actttgggat
      301 catggtattg ctggggcatt tcttgactac aatttatatg cctctcatta tgcaccacat
      361 cagggcgata attcacaaaa tataagttcc tatgggcagg ctggggttaa tcttggggcc
      421 tggcgcctgc gtactgatta ccagtacgat cagtcattta acaatggcaa aagccaggcg
      481 aacaacctgg attttccgcg tatttatttg tttcgcccaa tcccagcaat taatgcaaaa
      541 ctaactatag gtcaatacga tactgaatcc tctattttcg actctttcca tttttctggc
      601 gtttcgttga aaagcgatga gaatatgttg ccgcctgatc tacgtggtta cgcaccgcaa
      661 atcacgggtg ttgcacaaac gaatgcaaag gtcactgtct cacagaacaa ccgtattatt
      721 tatcaagaaa atgttcctcc tggcccattt gctattacta atttattcaa tacattacag
//
LOCUS       DAZOYL010000595          776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZOYL010000595 DAZOYL010000000
VERSION     DAZOYL010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.600"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..344
                     /locus_tag="ACHUOY_004350"
     CDS             <1..344
                     /locus_tag="ACHUOY_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0684901.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSRAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(406..>776)
                     /locus_tag="ACHUOY_004351"
     CDS             complement(406..>776)
                     /locus_tag="ACHUOY_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR0684902.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          191 c          175 g          200 t
ORIGIN      
        1 gtgcagatgg tcatttttgt ggcaatttgc cgaatacgac ccatttccat gagcaaaccg
       61 tggagttccc gatccaggga gagatggttg atattgtggc gcatggtgag ttgggcggcg
      121 acttttcgtt agtgcctgac agctacgtga cgatggggcc gaaaagcatc atggcggcga
      181 aaaacttgct gataattgtc agtcgcgcag gtaaagcgca ggcactgaaa aatgtgctgc
      241 aaggtccggt taccgaagat gttccagctt ccgtgttgca gctgcatcct tcgctgatag
      301 taatcgctga taaagccgct gccgcagaat tagcgctcgg ttaagctgaa gaaataaggc
      361 cggatgtatg aattacgctc cggcctgttc tcattattta aataactacg aaaaatcacc
      421 ccacagtctc agcttgccgc agtggagaca gcggaagaga taaccctgac aatcaccacc
      481 cttttgcagg catttagcta gatcagaatt tctaataccg aaattctcac aatcttcttc
      541 aaggttggca aattcatcga ggcgatcttt tatatcattc cagcccacat agccgataaa
      601 agcacagaaa tcgccacaat gcgcaagcca gaattcctgt tgccatccat gataacctgg
      661 cgtgcgttca accaactctt tcagcatttc atcaggatat gtgttcttaa taccggcaaa
      721 ttcgtcctct tcatcatact caaattcaac accttctatg ctggcatcat cctgaa
//
LOCUS       DAZOYL010000596          773 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZOYL010000596 DAZOYL010000000
VERSION     DAZOYL010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.601"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>773
                     /locus_tag="ACHUOY_004352"
     CDS             <1..>773
                     /locus_tag="ACHUOY_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0684903.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEP"
BASE COUNT          224 a          165 c          185 g          199 t
ORIGIN      
        1 gcagatgcaa aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggtgct tatcagcacg gaaggggcaa cagaatggtg
      121 cgcagaatac gatgagtggg gcaacctgct gaatgaagag aacccgcatc agctgcagca
      181 gcttatccgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcgata tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccgataact gatattgacc cgttgggatt
      361 agcgacctgt ttatacagca taactttaag tatgctttca tgcgtttcag atacgcaaaa
      421 tgatgataac tcttatgatg tgcttactat tcctgtcgcc tctggtaata atggtaataa
      481 tatgcaatgt aaaaataatc ctggttgtac acacttacaa aatagaggtc caatacctca
      541 aggggtatgg agctggaatg ttaatggtcc tggagcaacc aatagaaaac ctaatggtat
      601 acgtttagta ccgtcagcaa atacagaaac atataatcgt gatggatttt taacacaaag
      661 ttgtttaaac gctttcggtc cgtctttagg acctcgattc tgttccgagg gttgtataac
      721 tggttcatct aatgacatgc aaaaattaaa tgagttaata ttttcagagc cag
//
LOCUS       DAZOYL010000597          769 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZOYL010000597 DAZOYL010000000
VERSION     DAZOYL010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.602"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>769)
                     /locus_tag="ACHUOY_004353"
     CDS             complement(<1..>769)
                     /locus_tag="ACHUOY_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0684904.1"
                     /translation="KQTYPNFEIVIVNDGSKDNTSNVLREYGLTHSRLIIINKENGGV
                     SSARNTGIRKAQGQFICFMDDDDEIDPNYLLKMYTRQHETGGDAIYCGLYGHYIKDGV
                     TYSPINTEFNEGSLLFDFFYKKVRFHIGCLFIRKQLLEENNLFFDEDLRLGEDLDFIY
                     RLLITCDMYAVPYYMYKHNYRENSLMNSCRTITHYRHESFAHERIYSSVMQLYKGNRK
                     EEIHTLLSQNRAYHKTRYLWNVLLNGDFELLNQLVESN"
BASE COUNT          251 a          147 c          108 g          263 t
ORIGIN      
        1 cattgctttc aactaattga tttaatagtt caaaatcacc attaagtaga acattccata
       61 aataacgagt tttatgataa gctctatttt gactcaataa tgtatgaatt tcttctttcc
      121 tgttaccttt gtataactgc atcacagaag agtagattct ttcgtgcgca aatgactcat
      181 gtcgataatg agtgatggtt ctacatgagt tcattaagga attttctcta tagttatgct
      241 tatacatata atatggaacc gcatacatat cgcatgtaat tagcagtcga tagataaaat
      301 ccagatcttc tcctagtcgt aaatcttcat caaaaaaaag attattctct tccagaagtt
      361 gttttcttat aaacaagcac cctatatgga atctaacctt tttataaaaa aagtcgaaaa
      421 gtaaagatcc ttcattaaac tctgtattta taggtgagta agtaacacca tcttttatat
      481 aatggccata aagcccacaa taaatcgcat cccctcccgt ctcatgttgt ctggtataca
      541 tcttcagcag atagttagga tctatctcat catcatcatc cataaaacat ataaactgtc
      601 cttgcgcctt gcggatacct gtattcctgg cagacgaaac accgccattt tctttattga
      661 taataattaa tcgagagtgg gttaacccat actctctcaa aacgtttgat gtgttatctt
      721 tagaaccatc attaacaata acaatttcaa aatttgggta agtttgttt
//
LOCUS       DAZOYL010000598          764 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZOYL010000598 DAZOYL010000000
VERSION     DAZOYL010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.603"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>764)
                     /gene="mxiA"
                     /locus_tag="ACHUOY_004354"
                     /gene_synonym="virH"
     CDS             complement(<1..>764)
                     /gene="mxiA"
                     /locus_tag="ACHUOY_004354"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR0684905.1"
                     /translation="MGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAG
                     KIITTFGQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMS
                     IDADLKAGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGI
                     SVGMSQHGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNI
                     MSQIFGNPFVLIVTSALALAIGMLPGFPFFVFFLIA"
BASE COUNT          283 a          175 c          113 g          193 t
ORIGIN      
        1 tgctatcagg aaaaaaacaa aaaacggaaa acctggcaac atccctatcg ccaaggcaag
       61 agctgatgta acaatgagaa caaaaggatt cccaaatatc tgggacatta tgttacgtcc
      121 catattatcg ctatcaccat taactcttgt aacgataaat cctgcactta tagaaattaa
      181 cagagcagga atctgagaga ccaatccgtc accaatagtt agtatggtat aagtagataa
      241 tgcaccagat agggacattc catgttgact cataccaaca gaaatacctc ctattaaatt
      301 gacaaaaata ataatgatgc cagcaattgc atcaccttta atgaacttca ttgctccatc
      361 aaatgaacca tacaattgac tctcacgttc cagaatactg cgtctttctt tagctcctgc
      421 ggcatcaata attccagcct ttaaatccgc atcaatgctc atttgtttcc ccggcatacc
      481 gtcaagagaa aagcgagcag ctacctccgc aaccctttca gatccttttg ttataacaat
      541 gaactgcaca acagttacta ttgaaaaaat aacaaaacct acagccagac tatcaccaat
      601 gacaaactgg ccaaatgtag taataatttt acccgcatcc gcatcaacta aaattagtcg
      661 actagtactt attgataaag cgagacgaaa taatgtcgtt atcaacaaaa ccgaagggaa
      721 tgtagaaaaa ctcaatattc tttcaatata aaatgatccc ataa
//
LOCUS       DAZOYL010000599          753 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZOYL010000599 DAZOYL010000000
VERSION     DAZOYL010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.604"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>753
                     /locus_tag="ACHUOY_004355"
     CDS             <1..>753
                     /locus_tag="ACHUOY_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0684906.1"
                     /translation="KQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYLSESI
                     STLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNA
                     GVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTI
                     YTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKK
                     YPLKEARTVAEMQKQYKHLFAHGEYIKGITSLI"
BASE COUNT          238 a          144 c          143 g          228 t
ORIGIN      
        1 aaacaattta atacctcaat tattgtatat ttaattgacc caaaatactt tgctgatctg
       61 ccgacgtcac agttttggtc gtacgcgaca tacttcaggg tattgtcctt tgaatatctg
      121 agtgaaagta tttccacact gctgtatctg gatgccgatg tcgtttgtaa aggaagtctg
      181 aagcctctca cagaaattat atttaaagat gagtttgctg cggtcattcc tgacaatgtt
      241 agtactcagg cggcatgtgc aaaacgcctc aacattcccg agatgaatgg acgttatttc
      301 aatgcaggcg ttatctatgt caatattaaa aaatggcatg aagcaaattt gacaccgtat
      361 ttactcacgc ttttacgagg ggaaactaaa tatggctctc ttaaatattt agatcaggat
      421 gcgttgaata tcgcatttaa tatgaataat atctacctcg cgaaggattt tgatactatt
      481 tataccctga aaaacgaact tcatgatcgt agtcatcgaa agtatcagca aaccattacc
      541 gataaaacag tattgattca ctatacaggg ataactaaac catggcatag ctgggctggt
      601 tatccgtctg catcatactt taatatcgcg cgtgaacaat ctccctggaa gaaatatcct
      661 cttaaagagg cgcggactgt tgcagaaatg cagaaacaat ataagcatct gtttgcccat
      721 ggtgagtata ttaaaggcat aacttcatta att
//
LOCUS       DAZOYL010000600          753 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZOYL010000600 DAZOYL010000000
VERSION     DAZOYL010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.605"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..630
                     /locus_tag="ACHUOY_004356"
     CDS             <1..630
                     /locus_tag="ACHUOY_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFR0684907.1"
                     /translation="AYDIGFGGLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGA
                     VTKFGEHGKRKARKDLGVSMMMYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIA
                     YSPCEEHGYDLALSHDQMRQLTATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEET
                     LLHEQRFRRLNSQQPEVAEQLWKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT          206 a          178 c          200 g          169 t
ORIGIN      
        1 gcttacgata tcggctttgg cggtctggat catgtattga gtttgacgga aaacgtcaac
       61 attctggtgc tggatacgca atgctattcc aacaccggtg gtcaggcgtc gaaagcgaca
      121 ccgctgggtg cagtaactaa atttggcgag cacggcaaac gtaaagcgcg taaagatctt
      181 ggcgtcagta tgatgatgta cggtcatgtt tatgtggcgc agatttctct cggcgcgcag
      241 ctgaaccaga cggtgaaagc gattcaggaa gcggaagcgt atccgggacc atcgctgatc
      301 attgcttata gcccgtgtga agagcatggt tacgatctgg cactcagcca tgaccagatg
      361 cgccaactca cagctaccgg cttctggccg ctatatcgct tcgatcctcg tcgtgccgat
      421 gaaggcaaac tgccgctggc gctggattca cgcccgccgt cagaagcact ggaagaaacg
      481 ttacttcacg agcaacgttt ccgtcgtctg aattcgcagc agccagaagt ggcagaacag
      541 ttatggaaag atgctgcagc tgatttgcaa aaacgctatg acttcctggc acaaatggcc
      601 ggaaaagcgg aaaaaagcaa caccgattaa atgctctgga taaggattac ccaattctaa
      661 aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat cagtggcaat
      721 gcaatggccc aacagaaaat aaaatctgtg aca
//
LOCUS       DAZOYL010000601          748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZOYL010000601 DAZOYL010000000
VERSION     DAZOYL010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.606"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>748)
                     /gene="tssI"
                     /locus_tag="ACHUOY_004357"
     CDS             complement(<1..>748)
                     /gene="tssI"
                     /locus_tag="ACHUOY_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR0684908.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPG"
BASE COUNT          127 a          243 c          209 g          169 t
ORIGIN      
        1 ccgggtgtgc ggttttcctg gtggtaggta cgccccataa taatcggctg gtccgggtcg
       61 ccgttgagga agtccacaat cacctcctga cccacacgcg gtatcgccag gttgccaaag
      121 ccggtgcctg cccacgcctg tgccacacgg atccagcatg aactgtcctg gtttgacggg
      181 ttatagcggt cccagttaaa tttcacccgc acgcggccat gttcatcaca gaagatttcc
      241 tcgcctgccg gtcccgtcac gacggcgctc tgcgggccat ccaccagcgg tttcatcaac
      301 ggctgtggcc gccatgttct gtctgccggg attaccgcaa aatggttctc cagcgtggtg
      361 cctgaacccc tgcgtcccgg taccgcctgc ggctgttcgc cgtgaagatc gctcgccacc
      421 acctgccatt cccggttcag gttcgcctgc ggatgccccg tcagcacaat tcgccgtccc
      481 ggccatatct ccggcgaacg gcttgttccg cgcgccactt ctgcgttgtt tcgccagccg
      541 tccatctgcc agcgggcaaa gttctgcccg tgggcaccct tgaaacgtcc ggggtagtca
      601 tacacttcat actgcgtgcg ctggtaatcc tggtactggc cttcctgatc aaaacgccct
      661 ggccagccgg gacgtttaaa ggtgtagtct ttggtcacta cggaagaagg acggatttgt
      721 gcgctgtagc gaaactggct gatgcaga
//
LOCUS       DAZOYL010000602          748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZOYL010000602 DAZOYL010000000
VERSION     DAZOYL010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.607"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            5..748
                     /locus_tag="ACHUOY_004358"
                     /pseudo
     CDS             5..748
                     /locus_tag="ACHUOY_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
BASE COUNT          245 a          115 c          154 g          234 t
ORIGIN      
        1 gtaaaaaaat gtgtttttaa ttgagcaaat aaatgatctc aaaatgtggg tcaataagta
       61 tattgacgat tgcactgatg aggatttaaa cgatcgtgac tttattgcat cagttgttga
      121 ccgggctatt tttcatttcg cgattaatag tatatgtaat cctggggata ataaagatgc
      181 gactcccatt gaacaatgta cttttgatgt agaaactaag aatggccttc cctccacggt
      241 tcagctattt tatgaggaat ctaaggataa tgaaccttta gcgaatatac attttcaagc
      301 aataggttct ggttttttaa cgtttgttaa tgcctgccag gaacatgatg acaacagctt
      361 aaaattattt gcttcactgt taatttcact ttcatattct agtgcctacg cagatttatc
      421 agaaacagtg tatattaatg aaaataatga gagctacctg aaagctcagt ttgaaaaatt
      481 atctcaacgt gatatgaaga agtacctggg agagatgaag cgtctggctg atgggggaga
      541 aatgaatttt gatggctatc tggataagat gtcacatctg gtgaatgaag gaacgctcga
      601 tcctgatatt ttaagcaaaa tgcgagatgc tgcaccacaa ttaattagct tcgcgaagtc
      661 gtttgaccca acctcaaagg aagagattaa aatacttaca gacacttcta aattaattta
      721 tgatttgttc ggggttaaat cggagaaa
//
LOCUS       DAZOYL010000603          741 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZOYL010000603 DAZOYL010000000
VERSION     DAZOYL010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.608"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            54..>741
                     /locus_tag="ACHUOY_004359"
     CDS             54..>741
                     /locus_tag="ACHUOY_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0684909.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQ"
BASE COUNT          244 a          129 c          164 g          204 t
ORIGIN      
        1 acatgttaac gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac
       61 gtaaatgggc caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt
      121 atgcaaaatt cggtgtagaa atctatgctg ctgctaaaca aggtgaaccc gatccagaat
      181 taaacacatc tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg
      241 ttattgataa agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtgcagggac
      301 gttatgaagg ctttggtcct aatggctcaa tgattatcgc cgagacattg acttcaaatg
      361 ttaaccgtac gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag
      421 cggcaggttc tgtcagctat atgtttgaca atgctggtgt gattgtattt aaaggatcag
      481 accctgacca tatttttgaa attttacttg aagctgaagt tgatgttcgt gatgtgactg
      541 aagaagaagg taacattgtt atttataccg aacctactga ccttcataaa ggaatcgcgg
      601 ctctaaaagc agctggaatc actgagttct caacaacaga attagaaatg attgctcaat
      661 ctgaagttga gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg
      721 aagatgacga cgatgtacaa a
//
LOCUS       DAZOYL010000604          737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZOYL010000604 DAZOYL010000000
VERSION     DAZOYL010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.609"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..643
                     /gene="ydeO"
                     /locus_tag="ACHUOY_004360"
     CDS             <1..643
                     /gene="ydeO"
                     /locus_tag="ACHUOY_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR0684910.1"
                     /translation="FEPYERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNV
                     PTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNI
                     VNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSV
                     NKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
BASE COUNT          225 a          144 c          150 g          218 t
ORIGIN      
        1 ttttgaacct tatgagcggc attttaaaga gatcggattt actgaaaata ccattaaaaa
       61 atatctacaa tgcactaaca tccagacagt gacggtgcct gttcctgcga agtttttacg
      121 tgcttcaaat gtaccgactg gattgcttaa tgaaatgatt gcttatctta actcggaaga
      181 acgcaatcat cataattttt cagaactttt gcttttttct tgcctgtcta tttttgccgc
      241 atgcaaaggt ttcattacac tattaactaa cggtgtgcta tccgtttccg gaaaagtgag
      301 aaatattgtc aacatgaagc tggcgcaccc atggaagctg aaagatattt gtgactgcct
      361 gtacatcagt gaaagcctgt tgaagaaaaa actaaagcaa gagcaaacga cattctcaca
      421 gattctatta gatgccagaa tgcagcacgc aaaaaatttg atacgcgtag aaggttcagt
      481 caataaaatt gccgaacaat gtggttatgc cagtacatct tattttattt atgcgttccg
      541 caaacatttc ggcaacagtc cgaagagagt ttctaaggag taccgttgtc aaagtcacat
      601 gggtatgaat acgggcaaca cgatgagtgc tttagctatt tgattatttg ctaacgagta
      661 gtcaaccacg cacgctgcgt aagaattaaa tggggcagcc attccctgcc ccgcgttgtt
      721 tttaggcgat atattta
//
LOCUS       DAZOYL010000605          731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZOYL010000605 DAZOYL010000000
VERSION     DAZOYL010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.610"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>731)
                     /locus_tag="ACHUOY_004361"
     CDS             complement(<1..>731)
                     /locus_tag="ACHUOY_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR0684911.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAFL"
BASE COUNT          194 a          195 c          155 g          187 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
      661 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
      721 gattgctttc g
//
LOCUS       DAZOYL010000606          731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZOYL010000606 DAZOYL010000000
VERSION     DAZOYL010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.611"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHUOY_004362"
     CDS             <1..239
                     /locus_tag="ACHUOY_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR0684912.1"
                     /translation="ADRNRSHWSTSKTPSYTKSVSWQHHPLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            344..535
                     /locus_tag="ACHUOY_004363"
     CDS             344..535
                     /locus_tag="ACHUOY_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR0684913.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(536..>731)
                     /locus_tag="ACHUOY_004364"
     CDS             complement(536..>731)
                     /locus_tag="ACHUOY_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0684914.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
BASE COUNT          189 a          185 c          161 g          196 t
ORIGIN      
        1 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
       61 taagttggca gcatcacccg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc
      121 aactcgatgc ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg
      181 tcctttaccg tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat
      241 ccaaaaagaa cccgtcggga tggcgggtta tttgtcctgg ttattccccc gttgtaaaat
      301 ctctcctaaa cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact
      361 ttttgtttgc tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga
      421 acagcagtgt ctttattcta tgagcgatca acggatccgc catggcggtt gttttccgat
      481 tgaggatttt atagatggtt tctggcgacc tgcacaggag tacggtgatt tttaattatt
      541 gcaattgcac aagagtcagt tcgcccccaa agacagcacc ggtatcaata taatgcaggt
      601 tgccaatatc cacgcgatgt cgcaacggtg tatgaccaaa ccagaaatga tcagcacctg
      661 taattccctg ccctttttgg cgttcaccta atcgcgagcg gctccacaag acctgatgca
      721 aatcaacgtc c
//
LOCUS       DAZOYL010000607          730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZOYL010000607 DAZOYL010000000
VERSION     DAZOYL010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.612"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..311
                     /gene="ipaC"
                     /locus_tag="ACHUOY_004365"
     CDS             <1..311
                     /gene="ipaC"
                     /locus_tag="ACHUOY_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR0684915.1"
                     /translation="RRTYELNTLSAQQKQNIGRATMDTSAVAGNISTSGGRYASALEE
                     EEQLISQASSKQAEEASQVSKEASQATNQLIQKLLNIIDSITQSRNSTASQIAGNIRA
                     "
     gene            362..>730
                     /gene="ipaD"
                     /locus_tag="ACHUOY_004366"
     CDS             362..>730
                     /gene="ipaD"
                     /locus_tag="ACHUOY_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR0684916.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAEL"
BASE COUNT          281 a          163 c          115 g          171 t
ORIGIN      
        1 agagaagaac ttacgagctc aatacccttt ctgcgcagca aaaacaaaat attggccgtg
       61 caacaatgga cacatcagcc gttgctggta atatatccac atcaggaggg cgttatgcat
      121 ctgctcttga agaagaagaa caactaatca gtcaggccag cagtaaacaa gcagaggaag
      181 catcccaagt atctaaagaa gcatcccaag cgacaaatca attaatacaa aaattattga
      241 atataattga cagcatcacc caatcaagga attcgacagc cagtcagatt gctggtaaca
      301 ttcgagctta atatatccaa gagccataat aatatatggc tcttcctgta aggaaataac
      361 catgaatata acaactctga ctaatagtat ttccacctca tcattcagtc caaacaatac
      421 caacggttca tcaaccgaaa cagttaattc tgatataaaa acaacgacca gttctcatcc
      481 tgtaagttcc cttactatgc tcaacgacac ccttcataat atcagaacaa caaatcaggc
      541 attaaagaaa gagctttcac aaaaaacgtt gactaaaaca tcgctagaag aaatagcatt
      601 acattcatct cagattagca tggatgtaaa taaatccgct caactattgg atattctttc
      661 caggaacgaa tatccaatta ataaagacgc aagagaatta ttacattcag ccccgaaaga
      721 agccgagctt
//
LOCUS       DAZOYL010000608          728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZOYL010000608 DAZOYL010000000
VERSION     DAZOYL010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.613"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(55..351)
                     /locus_tag="ACHUOY_004367"
     CDS             complement(55..351)
                     /locus_tag="ACHUOY_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR0684917.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          213 a          159 c          148 g          208 t
ORIGIN      
        1 ggccaaacaa gaacacggtt gcaaaaaccg tgcccttaaa tattgaatct ctattcagaa
       61 cactttctta aatggtttca ctgaaacgtg ttcatagaca cctgccgcta cgtacgggtc
      121 agcatcggcc caggcctgag ctgcttccag cgattcaaat tcagcaataa cggttgagcc
      181 agtaaatccc gcagcccctg gatcgttact gtctaccgct ggcattggac cagctgtcaa
      241 caaacgacct tcatcatgca gtaactgtaa acgtgctaaa tgtgccggac gaacggaaag
      301 gcgtttttcg aggctatcag ctttgtcttg agcgtaaata acatacaaca cgggcacaac
      361 tccttgttcg ggaaagttgt aaagtacgtt atttgaaagg gcgaagatct gcaacggaaa
      421 gatgatgtct ttgttaagac catgcataaa gtaaggtcaa ttacgccaaa aatgacattt
      481 tcactgatcc tgatcgtctt gccttattga atatgattgc tatttgcatt taaaatcgag
      541 acttggtttt tctactgaaa tgattatgac ttcaatgacc cttgaatgaa gtctattcgg
      601 ttttctagga gacctgttaa aaacaggaca atatcaggtg agtccctatg caatttagta
      661 gtagatttcc aagtatctat gcaattctac acagattcta tacagtctcg gtatagtccc
      721 tttttacc
//
LOCUS       DAZOYL010000609          727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZOYL010000609 DAZOYL010000000
VERSION     DAZOYL010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.614"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..644)
                     /locus_tag="ACHUOY_004368"
     CDS             complement(<1..644)
                     /locus_tag="ACHUOY_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0684918.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIAS"
BASE COUNT          208 a          170 c          157 g          192 t
ORIGIN      
        1 tttgatgcaa taaaggcgtc gtcaatagaa gccggcataa cataaacgcc agggataact
       61 gacggaacga taaaatcttc cagtagttcc tcacgactca cgttctgcaa catagcctgc
      121 ggtgcggttg tatcaaccag ccccaccgca tgcaagtagt tcagaaacat agtggctgat
      181 gactggggat ctaaatcaag gagcagaata cgtaaatctt ctgacagcag gtgaggatga
      241 gcacgcaggg cgtgagctac ggaaacacta ctcacggttt tagacacacc gccttttaga
      301 ctaccgatga atatggaata tgcctcacta taacgatcgc gatatttagg aatgccacga
      361 tgtgcataga tatcgataat gttctggatg gttaatgcat attttgtcgc ggttcctgct
      421 gggcgttttt caaactgata gccctgagcc tccatctcac cgactgcacc atccacgctt
      481 gcgcgcgtta attttggaag cttagctaca gcagactttg aatagacttg ataaaaaaca
      541 tcagcctgaa actcctgctt ttgctcctga atctgttttg ttagggcgag caacatttta
      601 tctgcacgct gcgcaacctt acttaactgc tcaaaactag tcattccccc tccgtgctat
      661 gtaggatttt aacttattac aaaaaaaact gcattagcag ggtaatgcat gcaagtacat
      721 attgtac
//
LOCUS       DAZOYL010000610          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZOYL010000610 DAZOYL010000000
VERSION     DAZOYL010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.615"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHUOY_004369"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHUOY_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR0684919.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZOYL010000611          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZOYL010000611 DAZOYL010000000
VERSION     DAZOYL010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.616"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..399)
                     /locus_tag="ACHUOY_004370"
     CDS             complement(<1..399)
                     /locus_tag="ACHUOY_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR0684920.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEI"
     gene            complement(399..>726)
                     /locus_tag="ACHUOY_004371"
     CDS             complement(399..>726)
                     /locus_tag="ACHUOY_004371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684921.1"
                     /translation="SQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCADLNI
                     PFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVHWPD
                     GVAPPF"
BASE COUNT          176 a          212 c          183 g          155 t
ORIGIN      
        1 gatttccagt atcacatccg ctgcaatgcg gctcatctct gtcagtaacc accggttgtg
       61 gaacaccagg gaccagtact ggcagggaag tgtgaacaca atatgctgcc acgggcagtc
      121 ggggaccagg ctcagcagat actgtatcca ctgtgcgcca gccttcaccc cgcagtgcgg
      181 gcaggagcgg cttttacacc ggaagcagac cttttttgta tggcaacagt ccggtgatga
      241 acagcaccac tgtgtatacc ccatcagtgt ggtcccgcac gccatgattt tggtcaccga
      301 ctcaatcacc accggacgta ctgccctttc cggctgcttc tccagccagt taagccagtg
      361 gtttccctgc tgaaagatat cggcaaaacg gggaagcatc agaagggcgg ggcgactccg
      421 tccggccagt gaaccgtgcc acactccggg cagtacatac cgccggcgct gataccggaa
      481 agaatggtcg caaattcccg ctccgtgcag cgggcgattt ccggataccc ttcgtcatca
      541 acacgtacaa accagaagac cagctttttg tttcccgcat ccacaaagaa cggaatattc
      601 aggtctgcgc agcattcaac ggcatcgtca aaactatcaa agcgcagaac ttctgcgtct
      661 tcttcgtcaa aaaaatcatc ttcgtgaagc ttcacgacat agcggggaag tttgcttctt
      721 tgagag
//
LOCUS       DAZOYL010000612          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZOYL010000612 DAZOYL010000000
VERSION     DAZOYL010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.617"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(158..>726)
                     /locus_tag="ACHUOY_004372"
     CDS             complement(158..>726)
                     /locus_tag="ACHUOY_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0684922.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQI
                     HLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          225 a          179 c          157 g          165 t
ORIGIN      
        1 tggcttgata accggataac aacgtgcgct ttgttgatgc cggatgcggc gtgaattcct
       61 tatccggtct acaaaatttg caaatccaaa gcattacatt aagacgtggc ctgataaaca
      121 tatcgcatca ggccattttc gttagctatt gtccacgtca ggctttcaca tcactcactg
      181 caccgaccgg aatatcagca tgaacaggct cgggcggtac tttgcgatat ttatccagca
      241 gatgaatctg gatcctgcgc agcgtgtcgc cattgaggcg atagaaacgg aagtagagaa
      301 tcagcgttac catgaaaaac agagttggta gagcaatcat aataaactgc atacctaata
      361 gggcttgcgt agactgttca acattcggta catagccaat catccctaac acaaccgcaa
      421 tgaaaaaagc cgcaaaggct gaaccgccct tcaccaccat agtctgcacg gagtaagcga
      481 tactttcaca gcgtacgtgc agtttatatt caccgtaatc aacggtatct gccaccatga
      541 tgacctgtaa tacccagaaa agcgccgttc ccacattcag caaaataccc gcaatcacaa
      601 tgaggacgac gttgtgatag ctcattaatg ccattaacag gagaacacca cagcttaaca
      661 ccggaagaat agatgctccg gcccataaaa tgcgtcggga taatgattta actaagcgtg
      721 ggaaga
//
LOCUS       DAZOYL010000613          708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZOYL010000613 DAZOYL010000000
VERSION     DAZOYL010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.618"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..535
                     /locus_tag="ACHUOY_004373"
     CDS             <1..535
                     /locus_tag="ACHUOY_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0684923.1"
                     /translation="SLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDEDL
                     TVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTDL
                     LKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPGKD
                     ATPGNVYAQADYKIAYE"
BASE COUNT          217 a          148 c          154 g          189 t
ORIGIN      
        1 gtcacttctt gttgctggcg tagcaatgat tctttccaat aacgtatttg ctgacgaagg
       61 tcacggcatt gttaaattta aaggtgaagt tatctctgct ccatgttcaa ttaaaccagg
      121 tgacgaagac ttaactgtaa atctgggtga agtagcagat accgttctga aaagcgacca
      181 gaaatctttg gctgagcctt tcactattca cctgcaagac tgcatgttga gccagggtgg
      241 aaccacttat tcaaaagcga aggttacttt caccacagcg aatactatga cgggtcagac
      301 tgacctgctg aaaaacacca aagaaactga aatcggtggt gccacaggcg ttggcgtacg
      361 tattctggat agccagagcg gcgaagtgac tttaggtact ccggtagtaa ttacattcaa
      421 caatactaac agctatcagg aactgaactt caaagctcgt atggaatccc cgggcaaaga
      481 tgctaccccg ggcaacgttt acgctcaggc tgactataag attgcttacg agtaatcaca
      541 ttaggccggg ttcgcccggc cacatcatta acggattaat gataagtgga acagatgtat
      601 aaaaaattaa aattaaccac aataagcgaa ttgattaaaa atatttattg ttcattatcc
      661 gttctcatca ttggttgtgc gtcagcttat gccgttgaat tcaacaaa
//
LOCUS       DAZOYL010000614          705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZOYL010000614 DAZOYL010000000
VERSION     DAZOYL010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.619"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..217
                     /gene="vapB"
                     /locus_tag="ACHUOY_004374"
     CDS             <1..217
                     /gene="vapB"
                     /locus_tag="ACHUOY_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR0684924.1"
                     /translation="VFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWD
                     EWFDGHSVSADFMDNREQPGMQERESF"
     gene            217..615
                     /locus_tag="ACHUOY_004375"
     CDS             217..615
                     /locus_tag="ACHUOY_004375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR0684925.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
BASE COUNT          181 a          180 c          190 g          154 t
ORIGIN      
        1 cgtatttctc agcaaccgca gccaggcggt cagactgcca aaagcggttg cattgccgga
       61 aaacgtaaaa cgcgttgaag tgattgctgt cggacgaacc agaatcatta cgccagccgg
      121 agagacatgg gacgaatggt tcgacggaca cagcgtcagc gccgatttta tggataacag
      181 ggaacagccc ggcatgcagg aaagggagtc attctgatgc tgaagtttat gctcgatacc
      241 aacatctgca tttttacgat aaagaacaaa cccgccagcg tcagggagcg ttttaacctg
      301 aaccagggga gaatgtgtat cagttcggtc accctgatgg agctgatata tggtgcagaa
      361 aaaagccaga tgcctgaacg taatctcgct gtgatcgagg gatttgtttc ccgcattgat
      421 gttctggatt acgacgctgc tgcagccaca cacaccggcc agataagagc agaacttgcc
      481 cgtcagggac gccctgtcgg gccatttgat caaatgatcg caggtcatgc ccgcagtcgg
      541 gggctgatta ttgtgactaa taacacccgg gaatttgaac gtgtgggcgg cctgagaact
      601 gaagactgga gctgacctgt tcatcagaaa tcatctcccg gctcaacatc ctccccgcgt
      661 tcccggtgca cattgatgtt cacctcttca cgacgctgca tctgc
//
LOCUS       DAZOYL010000615          693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZOYL010000615 DAZOYL010000000
VERSION     DAZOYL010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.620"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..389
                     /gene="ratA"
                     /locus_tag="ACHUOY_004376"
     CDS             <1..389
                     /gene="ratA"
                     /locus_tag="ACHUOY_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR0684926.1"
                     /translation="YQLVNDVQSYPQFLPGCTGSRILESTPGQMTAAVDVSKAGISKT
                     FTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRIEFHLDFEFTNKLIELAF
                     GRVFKELASNMVQAFTVRAKEVYSAR"
     gene            379..669
                     /locus_tag="ACHUOY_004377"
     CDS             379..669
                     /locus_tag="ACHUOY_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR0684927.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
BASE COUNT          177 a          143 c          195 g          178 t
ORIGIN      
        1 tgtatcagtt agtgaatgac gttcagtctt atcctcagtt tttgccgggt tgtaccggaa
       61 gtcggattct ggagtccact cctgggcaga tgactgcggc ggtagatgtc tctaaggctg
      121 ggatcagcaa aacgtttact acccgcaacc agttgaccag taaccaaagt attcttatga
      181 atctggtgga tgggccgttc aagaaattga ttggtggatg gaagtttacg ccgctgagcc
      241 aggaggcgtg tcgtatcgag tttcatctcg actttgagtt taccaataag ttgattgaac
      301 tcgcctttgg tcgcgtgttt aaagagctgg cgtctaatat ggtccaggct tttacggttc
      361 gtgcgaaaga ggtttacagt gctaggtaaa attgccgttg aggtggctta tgcgctacct
      421 gagaagcagt acctgcagcg cgtgacgctg caggagggcg cgacggttga agaagctatt
      481 cgcgccagtg gcttgctgga attgcgtacc gatatcgatt taactaaaaa caaagtcggc
      541 atttacagcc gtccggcaaa actaagcgat agtgtgcatg atggcgatcg ggtggagatt
      601 tatcgccctc tcattgccga tccgaaagag cttcgcaggc aacgagcaga aaaatcagcg
      661 aataaataac agacagaaaa aaggtgctca ttg
//
LOCUS       DAZOYL010000616          690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZOYL010000616 DAZOYL010000000
VERSION     DAZOYL010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.621"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..565)
                     /gene="ypdA"
                     /locus_tag="ACHUOY_004378"
     CDS             complement(<1..565)
                     /gene="ypdA"
                     /locus_tag="ACHUOY_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR0684928.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT          207 a          167 c          181 g          135 t
ORIGIN      
        1 gcacaataaa gccgatacag acgctaccga ggatcattgg aatgccgatt ttagagacga
       61 tatcgatccc caacgcggta gttggtgccc agacaattac cagaatcatg gtcaacgtct
      121 cacacaacat gccgccgaga atactgacgc gccagcgctg tgctttgggg attttcaggt
      181 ttatccagcc tgatatacaa cccgccagaa tgctggtgat aaagcagggg atcgccgtca
      241 cgccgccgat atcaattaaa taccggtgaa tacccgcaat cacgccggta atgatgccta
      301 cccacgggcc aaacagaatc ccgtcggaca tcaccgcgat aatacgtacg ttcaccagcg
      361 agccttctac gggaacgccg gaccaggtgc tgaacagggc gaacagcgag aaaatggcgg
      421 taacggcgag caattctttt ggggagtgcg ccgacttgtg caacagttcg cgaaacaggc
      481 ggatacggat gagaaagaac aggcagataa gcattaacgc tgcccgatcg aagaccgcca
      541 gcagcatgtt gaatatttcg tgcacgggtg actctgaata aggattaaac ctctatgata
      601 ggtaacctga aggctgatga ccagcaggcc gtttttgagg aaaaaccata aatttaaaag
      661 gggattgaaa gataaatggt gtattagcaa
//
LOCUS       DAZOYL010000617          688 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZOYL010000617 DAZOYL010000000
VERSION     DAZOYL010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.622"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>688
                     /locus_tag="ACHUOY_004379"
     rRNA            <1..>688
                     /locus_tag="ACHUOY_004379"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          196 a          146 c          212 g          134 t
ORIGIN      
        1 aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc
       61 gagcgaacgg ggaggagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga
      121 aaggcgcgcg atacagggtg acagccccgt acacaaaaat acacatattg tgagctcgat
      181 gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa
      241 atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg
      301 cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg
      361 cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa
      421 ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag
      481 acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg
      541 accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa
      601 tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca
      661 tctccggggg tagagcactg tttcggca
//
LOCUS       DAZOYL010000618          688 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZOYL010000618 DAZOYL010000000
VERSION     DAZOYL010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.623"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            167..673
                     /gene="mug"
                     /locus_tag="ACHUOY_004380"
     CDS             167..673
                     /gene="mug"
                     /locus_tag="ACHUOY_004380"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR0684929.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVTYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT          171 a          156 c          193 g          168 t
ORIGIN      
        1 gcgattatga gtcgcctgct ctaaccactg agctaagggg ccatggtagc ggattataaa
       61 gtaactccgt gtcgcaatcc agccattacc gtgcgcctgc tgtttttata aacaatgtat
      121 tttcaatccg ttatactttt cctggtgatg ttagaaagga gtaaatatgg ttgaggatat
      181 tttggctcca gggttacggg tcgtattttg cggtatcaac cctgggcttt catccgccgg
      241 gactggtttt ccctttgctc atccagcaaa tcgcttctgg aaggtgacat atcaggccgg
      301 gtttaccgac cgtcagttga agccgcagga ggcacagcat ctgctggatt atcgttgtgg
      361 cgtcaccaaa ctggtagacc gtccaacggt gcaagccaat gaagtttcaa agcaggagct
      421 gcacgcaggt gggcgtaagc tgattgaaaa aattgaagat tatcagccgc aggcgttggc
      481 gattctgggc aaacaagcat atgaacaggg attcagccag cgcggtgcac agtgggggaa
      541 acaaacgctc accattggtt cgacgcagat ttgggtgctg ccaaatccca gcggtttaag
      601 tcgcgtttca ctagagaaac tggttgaagc gtatcgcgag ctggaccagg cgctggtagt
      661 gcgtgggcga taaaaaacgc caccgaaa
//
LOCUS       DAZOYL010000619          687 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZOYL010000619 DAZOYL010000000
VERSION     DAZOYL010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.624"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>687)
                     /locus_tag="ACHUOY_004381"
     CDS             complement(<1..>687)
                     /locus_tag="ACHUOY_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR0684930.1"
                     /translation="AEGWEDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPA
                     KFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQ
                     IKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQG
                     RLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLK
                     KLCVAVSFRS"
BASE COUNT          186 a          165 c          121 g          215 t
ORIGIN      
        1 tggaccgaaa ggagactgcc acgcataatt tcttcagccc tttccattca aacgtgaaat
       61 caataagttc atcagggaca tcgcaaacaa tatgaagacg gatttcttct ctgccgtgac
      121 tcttttcact gattgcgtaa ctgtcatgct ctggattatt taattctttc agcggaaatt
      181 tttcctcaaa ggctttatta agccgcccct ggtttccttt taccgcgaat aaataatcac
      241 cgccctgttt ttgtatcttc tctgcaatat ctttctggca tcccatcgca tcagttgtga
      301 tgatttttcc tttaatatcc agcatgttaa gaagttcagg aatggctgta atctcattgg
      361 atttctcatc cgtcttaatc tgcccgagaa ccagactgtg cattgttgag aacgcactaa
      421 tgacatgaat cgctcccttg cggcgactct tgtcataaga gtgccggagc gtttttccat
      481 caattgcaat gacgtcttta tcatctgaag aatggcagtc acgcatccag ttaataaagc
      541 actcgtgaaa ttttgcagga ctgatacagg atacaactct ggcaatagta tcgtgaacag
      601 gaataccatt ttcaaaatca ccatattgct tcaaaaaatc gagatgtgtt tcaccaaaat
      661 cctctatatc ttcccaaccc tctgcac
//
LOCUS       DAZOYL010000620          686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZOYL010000620 DAZOYL010000000
VERSION     DAZOYL010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.625"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(446..>686)
                     /locus_tag="ACHUOY_004382"
     CDS             complement(446..>686)
                     /locus_tag="ACHUOY_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684931.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          234 a          134 c          110 g          208 t
ORIGIN      
        1 taacgattta ttaaagatgc tctattaaga aaagccctaa acataatctg tgaagggctt
       61 tggaggtttg cttataacgt taacgaagtt tcaggaaaac tttctttaaa agctctgttt
      121 aaaaactctt aaagaaagcc ctaactcatg attagggcaa agctgtaaag gattcaataa
      181 tgaatgaaca aagacttttt gagatagcct ctcagaccaa cctgaaaggc taggacgaaa
      241 agcattgcac ctttccaact cagaatatcc attcatcgga ttattgaaag aaaaaataag
      301 agagaattgc tctcccccat atatacaatt atgtcatgct caaggttgtt tgtcaacagc
      361 ctttaacaga ttggtaacat ttatacttta atagatgcac ctgcaatcat accaatcaat
      421 acaaaacaga taatcaccaa ttccattaat gcaccacctt cgaatccgtc accagaatat
      481 tatcgctata caaaattgct atagccgcct tgagatattt ttcagggcta acgctgtatt
      541 tgttcgtttc ttcgataata agacgcttca tgtttgagtc ggtaacagga aacataaaac
      601 gcggttcacc agtttcaatt tcctgaataa agtcttcaat ttcttctgag ttttcagcca
      661 ttagctttag taatttatca aggtca
//
LOCUS       DAZOYL010000621          685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZOYL010000621 DAZOYL010000000
VERSION     DAZOYL010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.626"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..561
                     /locus_tag="ACHUOY_004383"
     CDS             <1..561
                     /locus_tag="ACHUOY_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0684932.1"
                     /translation="YRFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGK
                     DANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEV
                     PIQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILM
                     KIDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(617..>685)
                     /locus_tag="ACHUOY_004384"
     CDS             complement(617..>685)
                     /locus_tag="ACHUOY_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684933.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          154 t
ORIGIN      
        1 tatcgcttta gtcagcttga atctgcgttg aatacccaga agaactcgat tcctgcgctg
       61 gaaaaggaag tgaaagcgct tgataagcaa atggtggcgg cgcagaaagc agcggatgca
      121 tattggggaa aagatgcaaa cggtaagcaa atgacccgcg aagaagcctt taaaaaaatt
      181 caccaacagc gtgatgagtt taataaacag aacgatagcg aggcgttcgc ggttaaatat
      241 gacaaagagg tttatcaacc ggcgattgcg gcatgtcata aacagagtga agagtgttat
      301 gaagtgccga ttcagcagaa acgagatttc gatatcaacg aacaacggcg acagactttt
      361 ctgcaatcac aaaaactcag ccgtaaattg caggatgact gggtaacgct cgaaaaaggg
      421 caatatccgt taacgatgaa agtttcagaa ataaacagta aaaaagtcgc gattttgatg
      481 aaaattgacg atatcaacca agctaatgaa cgctggaaaa aagataccga acaactgcgg
      541 cgcaatgggg tgattaagta actcatgtta agtgcctttg ccggataata taaaaagtta
      601 tccggcaaat tattttttac attagcccta gtagctgtgg aatgaacaag gatatttgcg
      661 gaatgtaagt gatcatcatc aacgc
//
LOCUS       DAZOYL010000622          679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZOYL010000622 DAZOYL010000000
VERSION     DAZOYL010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.627"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..679)
                     /locus_tag="ACHUOY_004385"
                     /pseudo
     CDS             complement(1..679)
                     /locus_tag="ACHUOY_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like adhesin"
BASE COUNT          203 a          147 c          121 g          208 t
ORIGIN      
        1 tggcctgcgc cataggcttt aaatgaatac agataatttg atgagctgga taatttgatc
       61 tgttgagtta ctgttggatc aaagtttgta tctgtataga attcaattgt aagggtctga
      121 taaataccac ctacagccca gtatttacgg gtgctattcg cattattgca actccaatat
      181 aacacgtctt cgcttggatt ataccacgaa aaatattgtt ctgcagagtc accaatgtat
      241 atcccaggtg aactaacgtt agttacggta ccgtaagctg accacatgtt tgaaattaat
      301 aatgtgtaat acaaaccagg aactgatgta ttaaataatt tatgggagcc atacatttta
      361 cctgagtcaa ccattgcatt ttcaatacgc attgcgctac cacttgtgct atcacttctg
      421 ttgcaataaa gaaaaccacc accagaagcg gtatctaact gactctactg taattttaca
      481 cctgtaggtc caagctgcgt cggtttgtag aaaactaaat tatttgtact tgttgcatat
      541 ctgaatatga cagtagcatc gccattcggc cccgtttctc tccatttttg atctgacgga
      601 ccaacgccgt aactattccc aacaatatat cctacgttat catcagtgcc agcactcgcg
      661 gtataacaac tcaagcaca
//
LOCUS       DAZOYL010000623          677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZOYL010000623 DAZOYL010000000
VERSION     DAZOYL010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.628"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>677
                     /locus_tag="ACHUOY_004386"
     CDS             <1..>677
                     /locus_tag="ACHUOY_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0684934.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQ"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAZOYL010000624          674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZOYL010000624 DAZOYL010000000
VERSION     DAZOYL010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.629"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            303..638
                     /locus_tag="ACHUOY_004387"
     CDS             303..638
                     /locus_tag="ACHUOY_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR0684935.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          217 a          132 c          144 g          181 t
ORIGIN      
        1 atatttagtg tgttgttttt tatcagaata aataacgtct tctgatgcgt ttaaaacgtc
       61 agaaagataa aaatatcatg tgaataaaaa aaagaacaag tagagcatta acattatctt
      121 aaataataaa tagaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg
      181 atcgtcgtct aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg
      241 cctttttcgg caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc
      301 acatgtcatt accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca
      361 tgtacatctg cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg
      421 acgagctgat cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca
      481 tcattaaaga tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga
      541 aaaacatccg cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc
      601 cgatgaaact gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg
      661 gataaggcgc tcgc
//
LOCUS       DAZOYL010000625          670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZOYL010000625 DAZOYL010000000
VERSION     DAZOYL010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.630"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>670
                     /locus_tag="ACHUOY_004388"
     CDS             <1..>670
                     /locus_tag="ACHUOY_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR0684936.1"
                     /translation="TETPPPANSQPAAPAPEAPVAKAEAKPETPAQPVVDEQAVFDEK
                     MDVYIKCYNKLQIPVQNSLARYADWLKDFKQGPTGKESTVYGIYGISESNLAECEKGV
                     KSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYKDDAFAKGKTLHQTF
                     LKNLESFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYYSLAVMISAKQINNL
                     ISQE"
BASE COUNT          218 a          144 c          157 g          151 t
ORIGIN      
        1 aaacagaaac gcccccgcct gccaatagtc aacctgccgc accagctcct gaagctcccg
       61 ttgcaaaagc agaagctaaa cctgaaacac ctgcgcaacc ggtggtcgat gaacaagcgg
      121 ttttcgacga aaaaatggat gtctatatca agtgctacaa caagttacag atccctgtac
      181 agaacagtct ggcgcgttat gctgactggc tgaaagattt taaacagggg cctaccggta
      241 aagagagtac tgtttatggc atctacggca ttagtgaatc caacctcgct gagtgtgaaa
      301 aaggcgtaaa aagtgctgtg gcgttaacgc ctgcgctgca acctattgat ggcgtggcgg
      361 tgagttatat tgatgctgcc gtggcgttgg gtaataccat taacgaaatg gataaatatt
      421 acacccagga aaattataaa gacgatgcgt ttgcgaaagg gaaaacgctg caccagacat
      481 tcttaaaaaa tctggaatcc tttgaacctg tagcagaatc ttatcatgcg gcgattcagg
      541 aaattaatga taagcgccag cttgcggaac tgaaaaatat tgaagaaaga gaagggaaaa
      601 cattccacta ctactctctg gcagtcatga tttcagcgaa acaaattaat aacctgattt
      661 cgcaagagaa
//
LOCUS       DAZOYL010000626          669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZOYL010000626 DAZOYL010000000
VERSION     DAZOYL010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.631"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..595)
                     /locus_tag="ACHUOY_004389"
     CDS             complement(<1..595)
                     /locus_tag="ACHUOY_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684937.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLV"
BASE COUNT          195 a          155 c          160 g          159 t
ORIGIN      
        1 ccaccagaaa tgaggaaagg ctgagataca gtatatcacc agcagcctgc ttcatttttt
       61 ccagtaccag cgccgtctta ccacttcccg ccgagccaac aatcaccagc ggtagcgggt
      121 gttcgtacag tgcctgctga tcatcatcaa aggacagcat tttatccagg cggtggaaac
      181 gcccatgaga cggattgata tacgtcagcg cttcagtcgc gatatcaaca ggatccggca
      241 ctggttgttg ttgtaagcgc ccttcatcaa tcaccacgtt tctgcgaaga aaacgtgaag
      301 tgttataggc atgattgcga atatattcca gaacaaggca gatagtttca ccgtggtagc
      361 gatacagcga aaacagtaat cggtcccgga tactgagccg ggcgcggtaa agattggtgt
      421 caattttacg aacatcagcc tgaacaaagt tatcggtggc aatagcctgt gcaaattttt
      481 tataacccgg gatcgtgtca ggattcagag tatgataaag acagattttc atggctgccg
      541 gtttccgtcg tgcaaaaaac atgctgattt tacgaaggaa tgccttactg aacaaccttg
      601 cccggaatac cccccgaaat cagatggaac aatcagaaaa atgctctgca gttatggaat
      661 aaagaaggt
//
LOCUS       DAZOYL010000627          662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZOYL010000627 DAZOYL010000000
VERSION     DAZOYL010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.632"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..319
                     /gene="gatA"
                     /locus_tag="ACHUOY_004390"
     CDS             <1..319
                     /gene="gatA"
                     /locus_tag="ACHUOY_004390"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0684938.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
     gene            350..634
                     /gene="gatB"
                     /locus_tag="ACHUOY_004391"
     CDS             350..634
                     /gene="gatB"
                     /locus_tag="ACHUOY_004391"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR0684939.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
BASE COUNT          193 a          124 c          150 g          195 t
ORIGIN      
        1 agaattccct accgggataa tgcttgagca gcacgccatt gcaataccgc attgtgaggc
       61 gattcatgct aagtcgtcag ccatttatct gttaaggcca acaaataaag ttcattttca
      121 gcaagcggat gatgataacg acgtggaggt atcgttggtt attgcgttga ttgtggagaa
      181 tccgcagcag caattgaaac ttttacgctg tttatttggc aagttacaac agcccgatat
      241 cgtcgagaca ctaatcactc ttcctgaaac ccagttaaag gaatacttca caaagtatgt
      301 tttagattca gacgaataaa tccctctgta acaataaaaa ggactattta tgaaacgcaa
      361 gattattgtc gcttgcggag gcgcggttgc gacctctacg atggcggcgg aagaaattaa
      421 agagttgtgt cagaatcata gtattcctgt tgaattaatc cagtgtcggg ttaatgaaat
      481 agaaacctat atggatggtg tgcatttgat atgcaccact gccaaagtgg atcgtagttt
      541 tggcgatatt ccgttagttc acggcatgcc ttttatttct ggtgtcggta tcgaagcatt
      601 acaaaataaa attctgacta tcttacaggg gtgacctatg ttttcagaag tcatgcgtta
      661 ta
//
LOCUS       DAZOYL010000628          653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZOYL010000628 DAZOYL010000000
VERSION     DAZOYL010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.633"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>653)
                     /gene="tssI"
                     /locus_tag="ACHUOY_004392"
     CDS             complement(<1..>653)
                     /gene="tssI"
                     /locus_tag="ACHUOY_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR0684940.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPR"
BASE COUNT          112 a          213 c          180 g          148 t
ORIGIN      
        1 gcgcggtatc gcaaggttac cgaatccggt gcctgcccac gcctgtgcca cacggatcca
       61 gcatgaactg tcctggtttg acgggttata gcggtcccag ttaaatttca cccgcacgcg
      121 gccatgttca tcacagaaga tttcctcgcc tgccggtccc gtcacgacgg cgctctgcgg
      181 gccatccacc agcggtttca gcaacggctg tggccgccat gtccggtctg ccggtattac
      241 cgcaaagtgg ttattcagcg tggttcctga accactgcgt cccggcaccg cctgcggctg
      301 ctcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg cctgcggatg
      361 ccccgtcagc acaattcgcc gtcccggcca tatctccgga gaacggcttg ttccgcgcgc
      421 cacttctgcg ttgttgcgcc agccatccat ctgccagcgg gcaaagttct gcccgtgagc
      481 acccttgaaa cgtccggggt agtcatacac ttcatactgt gtgcgctggt aatcctggta
      541 ctggccttcc tgatcaaaac gccctgccca gccgggtcgt ttaaaggtgt agtctttggt
      601 caccacggaa gaaggacgga tttgtgcgct gtagcggaac tggctgatgc aga
//
LOCUS       DAZOYL010000629          652 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZOYL010000629 DAZOYL010000000
VERSION     DAZOYL010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.634"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(227..>652)
                     /gene="ospI"
                     /locus_tag="ACHUOY_004393"
     CDS             complement(227..>652)
                     /gene="ospI"
                     /locus_tag="ACHUOY_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR0684941.1"
                     /translation="AMLGYGVREAPVPNEISEYMTGFFHRHLEQIDSEGIVSHPNETY
                     SKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLDVQTGKGFNLYDPV
                     EKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          191 a          155 c           99 g          207 t
ORIGIN      
        1 gcgccacatg ggacaccgtc atgccaggct ccattgtctg ctgaataatg gcaatttttt
       61 cctgaggagt tttacgttta cgaagctcca gccccagcaa agttccagtc atctcaaaat
      121 tagaactagt gttagacata tatccaagcc tatctcttat tttaagatac atccactgtc
      181 tggcgtttca aggggccggc tcaaatctat gtacaggctc tcagcatcag caaagcctct
      241 tactcttcca actactactg ttagcatagt gatcaaattc ctgatcagat acatgaatta
      301 cctgaaccga actattttca tctacaaatg catctggcga tttttctaca ggatcataaa
      361 gattaaatcc ctttcctgtt tgaacatcca aaaacataat tttttcacca tcgttaaatc
      421 cagctatcca atgagtggcc tgttcaatag aaatcattac tacacttcct tttgaagtgt
      481 tttgtaaaat attttctgca atacgctcac gaaatttact ataagtttca ttaggatgtg
      541 acactatgcc ttcactatca atttgctcta aatgcctatg aaaaaatccg gtcatatact
      601 ctgatatttc attaggtacg ggagcctctc ttactccata ccctaacata gc
//
LOCUS       DAZOYL010000630          651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZOYL010000630 DAZOYL010000000
VERSION     DAZOYL010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.635"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..533)
                     /locus_tag="ACHUOY_004394"
     CDS             complement(<1..533)
                     /locus_tag="ACHUOY_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0684942.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRAT"
BASE COUNT          172 a          171 c          109 g          199 t
ORIGIN      
        1 taagttgctc tagagattcc tagctcttcc tgaatctgca acctcgtttt accttgtgaa
       61 ataagcatct gtacagcctc tgaagaagct tttacaggtc ttcccatatg taccccttca
      121 gctttagcac gttcaatacc agcctttcta cgctctgcta tcaagcttct ttcaagctgt
      181 gccaacgttg acataatgcc taacattgct tcacccatag cgcttgataa gtcaaatcct
      241 tctctaaggc tgataactgt cacaccttta gcctgtagcg ctttaaccgt tgataacaca
      301 tcaattgtgt cacgtccaag acggtctaca gctaccacaa ctacggtgtc accttctcgt
      361 gcataagcta acaaactgct gaaaccgttt cttaaagaag ctttgacaga accactgaca
      421 gcttcatcag cgaaccattt agcaatgttg taccctgcct cttcaatttg catttgctgg
      481 ttagtgatgg attggtcatc tgtactcacc ctgcaatatc caagtatcgc cataatgttc
      541 cccttagaag tctcaaaagt tagtctcata agcttagctg tctcataatc caaaaacaac
      601 cttttgagac tacttgaggg tgcattctac cgttacgttg aaagtgtctc a
//
LOCUS       DAZOYL010000631          647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZOYL010000631 DAZOYL010000000
VERSION     DAZOYL010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.636"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..497
                     /locus_tag="ACHUOY_004395"
                     /pseudo
     CDS             1..497
                     /locus_tag="ACHUOY_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            511..647
                     /locus_tag="ACHUOY_004396"
                     /pseudo
     CDS             511..647
                     /locus_tag="ACHUOY_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          162 a          177 c          170 g          138 t
ORIGIN      
        1 cagaacactg aaaatcggca tcaataccgt catacggaca ttaaaaagct ctcgccccgg
       61 cggataacat ctgcgccagt cgctcacgct gatgttgccc ttatctgtga acttgatgag
      121 cagtggcgct tcgttggcag caaggcgcaa caacactggc cctggtatgc gtacaacact
      181 aaaactggcg gtgtgttggc ttacacttgc gggccacgga atgatgagac ctgtcgggaa
      241 ctgttagccc tgttgactcc gttctgtatc ggcatggtgg catggtgacc agcgatgact
      301 ggggcagcta cgccagagaa gtgccgaagg agaaacacct gaccggcaaa atcttcacgc
      361 aacgcattga acgtaacaac cggaccctcc gcacccgcat caagcggctg gcacgtaaaa
      421 caatctgctt ctcgcgttcc gtggaaatcc acgaaaaagt catcggctcc ttcattgaaa
      481 agcacatgtt ctactgattg gaagcactac ccccgcctgt tgcgacagtc acgtcatcgc
      541 cgtccgttac cggaacatgt ttcccgcgaa atacaccatc tggagcctga agaaagctgt
      601 tgtccggagt gtggtggtga actggattat tcgggggaaa tcagcgt
//
LOCUS       DAZOYL010000632          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZOYL010000632 DAZOYL010000000
VERSION     DAZOYL010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.637"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="ACHUOY_004397"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="ACHUOY_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0684943.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAZOYL010000633          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZOYL010000633 DAZOYL010000000
VERSION     DAZOYL010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.638"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..568)
                     /gene="ppiA"
                     /locus_tag="ACHUOY_004398"
                     /gene_synonym="rotA"
     CDS             complement(<1..568)
                     /gene="ppiA"
                     /locus_tag="ACHUOY_004398"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR0684944.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVL"
BASE COUNT          169 a          153 c          165 g          153 t
ORIGIN      
        1 gcaggacttt agcggaaagg ataactaccg gttttgacgg cacattctgg tacggaccaa
       61 catcatgagt cggcacctgg gaaatcttat cggcaacgtc catgcctttc accactttac
      121 caaataccgc gtaaccgaaa tcacgctgac catggtcaag gaaggcgtta tcggcaacgt
      181 tgataaagaa ctggctggtg gcgctgtctt tgtcagcggt acgcgccatc gcgatggtgc
      241 cacgcgtgtt gcgcaggccg ttatcggctt catttttgat tggcgggttt ggttttttct
      301 gctgcatctg ctcggtgaaa ccgccgccct gaatcataaa gccaggaatg acgcggtgaa
      361 aggtagtgtt gttataaaaa ccgctgttca cataatcgac aaagttttgc acagacactg
      421 gcgctttttg tttatccagc tccagttcga tgttaccagc tgaggttgtc aacaatacgt
      481 gcgggtcccc tttcgctgcc attgctgcgg gagaaagagc agaaagagcg aaaacagcag
      541 ccatcgccgc cagggtcgat ttgaacatga gatttcctta acaaacagag cagaaaaaaa
      601 gcaagtgaag agattctaaa tagccttcaa gaacaaggcc
//
LOCUS       DAZOYL010000634          636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZOYL010000634 DAZOYL010000000
VERSION     DAZOYL010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.639"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..348
                     /locus_tag="ACHUOY_004399"
     CDS             <1..348
                     /locus_tag="ACHUOY_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain-length determining protein"
                     /protein_id="HFR0684945.1"
                     /translation="DYRSSKGGFDNAQSSYKFLLGEKLLSAELKATKDAPIIYPFRYY
                     EVKRQIDELEGMLRDNIQAQAYRYQMKPSEPVIKDKPNKALILILGALPGAMFAIVGT
                     LVYATLKDKTKLD"
     gene            417..>636
                     /locus_tag="ACHUOY_004400"
     CDS             417..>636
                     /locus_tag="ACHUOY_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HFR0684946.1"
                     /translation="MKFDTLNAKIGIIGLGYVGLPLAVEFGKKVTTIGFDINKSRIDE
                     LRNGHDSTLECSNLELLEATKLTYACSLD"
BASE COUNT          213 a           93 c          139 g          191 t
ORIGIN      
        1 gattatcgct ctagcaaagg cggcttcgat aatgcgcaaa gtagctataa gttcttgctc
       61 ggcgaaaaac tgttatcagc agagctaaaa gcaactaaag atgcgccaat tatttaccca
      121 tttagatatt acgaagtgaa acgtcaaatt gatgagttag aaggaatgtt acgcgataac
      181 attcaggcgc aagcatatcg atatcaaatg aagccatctg agccagttat aaaagacaaa
      241 cccaacaaag cattaatttt gattcttggt gcattaccag gggcaatgtt tgctatagtt
      301 ggtacattag tttatgcgac attaaaagat aaaaccaagt tagattaaac tgggttacgt
      361 attgttgtgt caatgcgaaa tagatgttct atgtgcactt tatgatggat aagaaaatga
      421 aattcgatac tttgaatgcg aaaattggga ttataggcct tggttatgtt ggattgcctc
      481 ttgctgttga gtttggaaag aaagtaacga cgattggatt tgatattaat aagtctcgta
      541 ttgatgaatt acgaaatggt cacgatagta cattagagtg ctcaaattta gagttgttag
      601 aagcaactaa attgacgtac gcctgttcat tagatg
//
LOCUS       DAZOYL010000635          622 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZOYL010000635 DAZOYL010000000
VERSION     DAZOYL010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.640"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..427)
                     /locus_tag="ACHUOY_004401"
     CDS             complement(<1..427)
                     /locus_tag="ACHUOY_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0684947.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRG"
BASE COUNT          165 a          130 c          142 g          185 t
ORIGIN      
        1 gtccacgaat gtgatttatc agttgtgtcc tttccattga aaccaaccga cgcatattca
       61 aaaaagtctg gacgtcctgt tgctccagag tttttattgg tacgggtcgt atagttccat
      121 gttggcaggc ctcagctatt gcctgtgcgt cgttataatc atttttctgg caccgctgat
      181 aagctttaac atgttgtgcc ggaagcaata ttacatcatg gtcgagtttc ctgatttccc
      241 gggcccagta atgagatgcc ccgccagcct ccatcgcaac agtcatttta ggctgttgca
      301 ccagccagtt aagcagttga ttgcgagaaa gtttggtctt tcctgcgggt ttgccatgag
      361 cattgatact gaaaagataa aagttcgttt tagccagatc aatgccggtg attttctgtt
      421 cagacatggt attccgctta cttttgtggt taaagtttat ccatcatggc gcaattgaag
      481 gctgccgtga aagaggggga agactatctc atcggttact gttaaacggc taagagaaac
      541 tatctttttc aagcaaagaa gatacggtga aacaggcgat ttcaccgtga aatactgttc
      601 aaaaaaaacg ctaataaaag ca
//
LOCUS       DAZOYL010000636          616 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZOYL010000636 DAZOYL010000000
VERSION     DAZOYL010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.641"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..271)
                     /locus_tag="ACHUOY_004402"
     CDS             complement(<1..271)
                     /locus_tag="ACHUOY_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFR0684948.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHD"
     gene            complement(274..>616)
                     /locus_tag="ACHUOY_004403"
     CDS             complement(274..>616)
                     /locus_tag="ACHUOY_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0684949.1"
                     /translation="SSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYI
                     QVAEELGFYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLL
                     AFHKGPRLPDD"
BASE COUNT          168 a          142 c          104 g          202 t
ORIGIN      
        1 tatcatgccc actgtgacaa acgattgcat tgggctgaaa accatgaact attccgagta
       61 atctccatgc agtagggatg tgaagactgt ttcgaaataa cgcgaatgtg atatcaattc
      121 ccaatttact ggcttcaaaa gcaattttgc tgttttccct gcagacaagc agaactgaat
      181 gccccatttt cttcagggcc ttcatttgag caacagcctg taattcctga ccacctatat
      241 tcggtgatga ttccgtaaat agtatattca ttattaatca tccggtaatc ttggcccctt
      301 atgaaaggcc agcaacgatg aaaaaaacgg tgttgtataa taaaaaagtc gtcttttcag
      361 ccatgcgcaa ctttcccgtt ctttggtgct tattcttcct atccgtgtag ttcctttagc
      421 aggagcattc attcttcttt ctgttgtata taaataatag aatccaagtt cttcagcgac
      481 ctgaatataa tccttgttat aataaccttc cggccaacac aaatgtttac tgcattttcc
      541 tgtcatttct ttcaaatatt ctctcccgct caacaagtcc tgccgaagat gtttacactg
      601 ttcttctcgt gaactg
//
LOCUS       DAZOYL010000637          603 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZOYL010000637 DAZOYL010000000
VERSION     DAZOYL010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.642"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..303
                     /gene="phoN2"
                     /locus_tag="ACHUOY_004404"
     CDS             <1..303
                     /gene="phoN2"
                     /locus_tag="ACHUOY_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR0684950.1"
                     /translation="ENMAITGSYPSGHASFGWAVALILAEINPQRKAEILRRGYEFGE
                     SRVICGAHWQSDVEAGRLMGASVVAVLHNTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            376..498
                     /locus_tag="ACHUOY_004405"
     CDS             376..498
                     /locus_tag="ACHUOY_004405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684951.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
BASE COUNT          172 a          134 c          151 g          146 t
ORIGIN      
        1 gagaacatgg ctatcactgg ctcttatccc tctggtcatg catcctttgg ttgggcagta
       61 gcactgatac ttgcggagat taatcctcaa cgtaaagcgg aaatacttcg acgtggatat
      121 gagtttggag aaagtcgggt catctgcggt gcgcattggc aaagcgatgt agaggctggg
      181 cgtttaatgg gagcatcggt tgttgcagta cttcataata cacctgaatt taccaaaagc
      241 cttagcgaag ccaaaaaaga gtttgaagaa ttaaatactc ctaccaatga actgacccca
      301 taaagctgga cagcctgtat caggctatgg agggcccata gacaaatcta ccctatatga
      361 gcataggagg agtctatggg cacaccacgt tttacccctg aatttaaggg attactggaa
      421 aggctgggac atatccctcc ggcagaagca gaaaaagctt attatgctgc catcggaaac
      481 gatgatctgg caacctgagt tcacagataa aacattctct aggaaactcg gggcggttcc
      541 gttcaccaca tgcaatgtgg tgttgcaggg gaacggtctg caccatcccc tatgtcgatc
      601 aat
//
LOCUS       DAZOYL010000638          601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZOYL010000638 DAZOYL010000000
VERSION     DAZOYL010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.643"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..516)
                     /locus_tag="ACHUOY_004406"
     CDS             complement(<1..516)
                     /locus_tag="ACHUOY_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR0684952.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDF"
BASE COUNT          180 a          117 c           76 g          228 t
ORIGIN      
        1 aaaatcactt tttcttatat tatcgaatag ggtctgttca tttctcttag cgcattcaat
       61 aaactgatca acattatttt tattcgatga caggtcagtt tcagaaaccg cattttcttt
      121 gttctccagc tttagtttaa tgagaagatc ttcccagacc tgcttgctta aacatactac
      181 gtcaggctca ccatcagtaa ctaactcttc attattatca aagtatacgt tgaatacttt
      241 taagttattt tcatcaacat catatttaat acgttttaat tgctctccag atcccataat
      301 gacaaaggcg ttgccttcat gatatataca ttcagacatc attttttgta aagtttcaag
      361 agcaccgcga tacgtccctg aggcagcttc cttacaaatt aaatttataa tactataagc
      421 tagtagctcc atggattcag aagattttct ttcattacaa tttccactat ctataaatga
      481 gctagtgtta aattcgttgc ttttactaac taacattgtc tattcctcag ttaatgtcta
      541 catggctatt tttaatttta ttattgtttg tcactataaa aaatcgctca tttgagacaa
      601 t
//
LOCUS       DAZOYL010000639          597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZOYL010000639 DAZOYL010000000
VERSION     DAZOYL010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.644"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHUOY_004407"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHUOY_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0684953.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          130 a          162 c          171 g          134 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc cgtgccggaa cgttacctca cagctgatac tgagggtttt cgggtcctct
      541 gcgagcgatt ctggctgttc ggcagctgca tcgagagtca caggaagtag ctggggg
//
LOCUS       DAZOYL010000640          594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZOYL010000640 DAZOYL010000000
VERSION     DAZOYL010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.645"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..311
                     /gene="tnpA"
                     /locus_tag="ACHUOY_004408"
                     /pseudo
     CDS             1..311
                     /gene="tnpA"
                     /locus_tag="ACHUOY_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          182 a          140 c          120 g          152 t
ORIGIN      
        1 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
       61 aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      121 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg tgactgcgac
      181 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtgttggct
      241 attttgccag tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      301 agcaaacacc tggttaggtg gaaaaccacg ccttatatcc ctgccctgaa ggacggggtt
      361 ttacggcgca ccggataagc ctggagatta tctacaccag catggacatc attgtactat
      421 gccccgtaca tgccccatca catcctcaac atcaaagtat tagagccttg caaaccatta
      481 tgcgtctcag tttgtctcat ctatcttacg caaaaaaaaa tagaactaat atcaagcggc
      541 gctatattgc gtattaaata aaaccacctc aattacatta ataaatctaa acaa
//
LOCUS       DAZOYL010000641          594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZOYL010000641 DAZOYL010000000
VERSION     DAZOYL010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.646"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..521)
                     /gene="tnpA"
                     /locus_tag="ACHUOY_004409"
     CDS             complement(<1..521)
                     /gene="tnpA"
                     /locus_tag="ACHUOY_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR0684954.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FKVRLAKQALQPGAVVARIAREHDINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPF"
BASE COUNT          145 a          134 c          155 g          160 t
ORIGIN      
        1 cagaagggat ttgtaactgg tctggtcggc tccggcgtat cagtcagtgc caccgggaca
       61 ggcatgcatt cctgtatgtc atcatcgctc agtaagccgt cctcgtactg gcttttccat
      121 ttaaacagca ggttatcatt gatatcgtgt tctctggcga tccgggcaac aacagcacca
      181 ggctgtaatg cctgcttagc cagacggacc ttaaattcac ggctatagct ggttcgccgt
      241 tcttttcgcc atgagccttc tctgatttga ggctctgtta attccttctt tctgttggca
      301 taaaggatgg cgtcaagttg agcgaatgaa actgaatcgg gcaatggcca tgcgataccg
      361 gatgcaagaa atcgctgaaa aagcgtatgt attgtggaat gactgagacc cagacgctga
      421 gcgatggccc ggatggtcag tttatcttca aatcttaaac gcagggcatc aggcaaataa
      481 gaacggaagc agggaatatc ttttgttgtc tgggaattca tcgttcgtgt ccatcaatat
      541 agatgggcgc gattgttgcc agacaggaca attttcacaa gacgtcgctg atgg
//
LOCUS       DAZOYL010000642          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZOYL010000642 DAZOYL010000000
VERSION     DAZOYL010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.647"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHUOY_004410"
     CDS             complement(<1..396)
                     /locus_tag="ACHUOY_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0684955.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          176 a          148 c           97 g          172 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggc
//
LOCUS       DAZOYL010000643          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZOYL010000643 DAZOYL010000000
VERSION     DAZOYL010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.648"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHUOY_004411"
     CDS             <1..91
                     /locus_tag="ACHUOY_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684956.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUOY_004412"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUOY_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0684957.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZOYL010000644          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZOYL010000644 DAZOYL010000000
VERSION     DAZOYL010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.649"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..60)
                     /locus_tag="ACHUOY_004413"
     CDS             complement(<1..60)
                     /locus_tag="ACHUOY_004413"
                     /inference="COORDINATES: protein motif:HMM:NF015750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0684958.1"
                     /translation="MASVSISYPSCSATDGVVRN"
     gene            116..593
                     /locus_tag="ACHUOY_004414"
                     /pseudo
     CDS             116..593
                     /locus_tag="ACHUOY_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
BASE COUNT          146 a          132 c          134 g          181 t
ORIGIN      
        1 gttacgcacc accccgtcag tagctgaaca ggagggatag ctgatagaaa cagaagccac
       61 tggagcgcct caaaaacacc atcatacact aaatcagtaa gttggcagca tcaccgccgt
      121 tgtctacaca tttattctct cggtgctgat ttaccgggaa gtaaaatggc gccatttacc
      181 caaactgatt ctggagtcgg ttgttaccac ttcgattgta ttactgttga tcggtttttc
      241 cgtgggcatg tcctgggcaa tgactaatgc tgatattcct tatatgatca gcgacgcatt
      301 gatggggatt tctgataacc cactgatcat attgctgctg attaatatcg tcttattaat
      361 tgtcggcatc tttatggaca tgacaccggc tgtactaatc tttacaccga tcttcttgcc
      421 gattgcacaa gagctgggaa tggacccagt acatttcggt attatgatgg tggccaacct
      481 ttgtattggg ctgttaacac cgccggtggg tagtgcgcta tttgttggtt gttctatctc
      541 gggtgtgaag attcaacatt taatcaaacc attgctgcca ttttatgcta gca
//
LOCUS       DAZOYL010000645          591 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZOYL010000645 DAZOYL010000000
VERSION     DAZOYL010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.650"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..395
                     /locus_tag="ACHUOY_004415"
     CDS             <1..395
                     /locus_tag="ACHUOY_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR0684959.1"
                     /translation="INAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSLAA
                     VLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVLD
                     GKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT          214 a          109 c          108 g          160 t
ORIGIN      
        1 tgattaatgc cattgtcaaa ccgttctatg aacgtcagat gagtaagtca gataatccaa
       61 ttgataaagt tgtaaggtca aaggtggaac ccacagacca agcattgact ggtgacatga
      121 actctttagc cgctgtttta tgggctatgg ttcagggcaa atcaaaagat gttccacagt
      181 tttcacctga agcattaaat aatctgaaag gtcgagtgga aacattcaca caagatttaa
      241 aggttatcaa accgtcagta aatattcagc ctcagacaat caatatcaca actcagacgg
      301 tacttgatgg aaaggttatt gatgaaagaa ccacatcgca tattaatcgt atgcaggaag
      361 ataccttgat tagctcagct tatccagagg aataaacaat gttagagcta ctaatattta
      421 ttgccacaca tggcaatcta ttaatcttta tctagtatga cgattcaact atgaatagtc
      481 gaatgctacg tgaaatcttc tgaacagata ccaacatcaa aaattagatt tcacgtagct
      541 cacgcttaac agaataggaa taacaacaat gtttgacaca aagcaaatta g
//
LOCUS       DAZOYL010000646          586 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZOYL010000646 DAZOYL010000000
VERSION     DAZOYL010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.651"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          130 a          141 c          161 g          154 t
ORIGIN      
        1 aatcatattt aatcatagtg attactttca gacggaacaa ggcaaataat gcgagtcagc
       61 gcatataagg cggcatacgg tggttacatg tagcacaggt gtaaaaccgc cgaagcccgg
      121 cgcaagccgg tacggtcgtc cgaatatcac cagtccccct ccggctctgc gaggccatgc
      181 ggcctgtcag cgatgaaaat gcctctgtgc gcggtcggag tggtcccgac aagggtttac
      241 ccgcagtcgg ggcgtatctc cgcgttagcg ggccgtgagg gccgcttacg agcgtgtatg
      301 agagacgtac agtagttttg tccgctcaca ccagtacaaa ccagtcgaaa taccggtaac
      361 gacgattact cttgtctctg atgccggtgg actacgagat tttatttact ggcgtcctga
      421 tgcggtcagg actggtatgg aacctgttta tgtgatactt aatgctcttc tggattccgg
      481 gcgatttacg cgtaaactta ttctgttagg gctgtcaggc tcattttcct atatcttcgg
      541 ctcaattgtt gcgacactgg gaatgggact tgttgtcgat tatctg
//
LOCUS       DAZOYL010000647          585 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZOYL010000647 DAZOYL010000000
VERSION     DAZOYL010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.652"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>585)
                     /locus_tag="ACHUOY_004416"
     CDS             complement(<1..>585)
                     /locus_tag="ACHUOY_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0684960.1"
                     /translation="GNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYG
                     YDGYGGNAITGDNLSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQN
                     VVGNAIHITGGNNSLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDC
                     TTVSLSGNKFTVSGDVSFGENSSLNLAGISSLEAS"
BASE COUNT          171 a          147 c           92 g          175 t
ORIGIN      
        1 agaagcttcc agactactga ttccagctaa atttaaagaa ctgttctcac caaaagaaac
       61 gtcacctgaa acagtgaatt tgttacctga tagtgaaaca gttgtacaat caccagcaca
      121 taaatcacct tcaatagttg gtgtggtccc agtgtaatca ttgttgataa ttttcagaat
      181 ggatgaattg ttaacctgta catcaccagt aatgacagaa ccttcatgga gtattaatga
      241 attgtttcca ccagtgatgt ggatagcatt acctactaca ttttgtgttc catcatcttc
      301 tttacctgaa attatatatc cgctattagc aatggtcata ttgctaccat ttatagcatc
      361 cccccaatgt ccaccattac cgcctaaaat agctccattg ttgattatag acaggttatc
      421 tcctgtgata gcattaccac cgtagccatc ataaccataa ccgtaagcag cacctccgtt
      481 gcctccagcg aacgtgccct tattgattac agagaggctg cttcctgtga tggcatcacc
      541 tccattaccg ccatcaccct caccataatt gttacctcca ttacc
//
LOCUS       DAZOYL010000648          581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZOYL010000648 DAZOYL010000000
VERSION     DAZOYL010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.653"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          158 a          113 c           93 g          217 t
ORIGIN      
        1 tgttatttat acttatcggg tatcagtttt gataggggta tcagacttga taggggtaac
       61 agaattctgt gggggtagtt cactatgacc tacccctagt tcactgtgag ctaccgttac
      121 atccaatcat cttccgatgt aacgtatcgt tactaccgat gtgtaatatc gttacattca
      181 ataattagtc tatgtaatat atcgttacta ccaactttct tacagagagc atttcagata
      241 aatgaatgta acctctcgtt acatccattt gtcacatagc actctgtgta atgtctttct
      301 atgtgtgtgc cataatcccc ctatattccc tgtgtaaatc atagaataat gctctctatt
      361 ccctgtgtaa ttccctacta aaaaatccaa tgaaaagtag cttaaaagtt caattcttac
      421 tttcttcact tttacatcac tttcgcaaag ccttgtgtaa tgcggcttac agagcaccat
      481 cagaaattgt tggctaaaaa ttgttctgtt tgtttctgaa cgagtttctc gtttgtgcaa
      541 attttaaaat ttattttgtt tttatagcct tatttcgtgt a
//
LOCUS       DAZOYL010000649          576 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZOYL010000649 DAZOYL010000000
VERSION     DAZOYL010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.654"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..576
                     /locus_tag="ACHUOY_004417"
                     /pseudo
     CDS             1..576
                     /locus_tag="ACHUOY_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
BASE COUNT          214 a           97 c           81 g          184 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttca
//
LOCUS       DAZOYL010000650          576 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZOYL010000650 DAZOYL010000000
VERSION     DAZOYL010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.655"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>576
                     /locus_tag="ACHUOY_004418"
     CDS             <1..>576
                     /locus_tag="ACHUOY_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684961.1"
                     /translation="EQQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGS
                     TISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDG
                     QVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFAT
                     KNVLAFSRGDSVNINHSPHDGLVIINKGNEEV"
BASE COUNT          185 a          114 c          113 g          164 t
ORIGIN      
        1 tgaacaacaa atgtcatcac tgctttatga aagagatgat ttaagcgcca ttgatttttc
       61 attgctcatg aagacgataa aagccttttc attcggggga gatctcctaa cccttgcatc
      121 aaaaccgggt tccacaatct caagcatccc atcagaaaga cggatattga ttaacattaa
      181 ccacgatttt cctaacaatg gcaatctttt caatgacttt ctatttaacc atcaacaaga
      241 tgaacaatta gcaatggcat atatggccgc tctcccgttc agcagacctt tagtttactg
      301 ggatggccag gtattaaaat caacgactga aattaaaaat tatgatgggt ccacgcgtgt
      361 cggtggtgag gcgtggctta ataaaggttg ctctacctat cagcagctct acaatgaatt
      421 ccacgcatta tatatagata aagcaggaat atggagcgca tttgagggtg tatttgcaac
      481 taaaaacgtt ctagccttta gtcgtgggga ttctgtgaac attaatcact ctcctcatga
      541 tggactagtt ataataaata aaggaaacga agaagt
//
LOCUS       DAZOYL010000651          575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZOYL010000651 DAZOYL010000000
VERSION     DAZOYL010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.656"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..517)
                     /locus_tag="ACHUOY_004419"
     CDS             complement(<1..517)
                     /locus_tag="ACHUOY_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0684962.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          161 a          137 c          115 g          162 t
ORIGIN      
        1 atgggttgct ggcatcattg cctttcgcat cacccggaca gttagaagag gctacccacc
       61 aagcattttt atataccgca ccattaaaga cgacatacca ggtctgaccg ccctgctgtt
      121 tattccaggc aactacagaa gaattagacg gaacagtgcc ctgtgctggt gtagccgaac
      181 cgccattagc cggcgtattg gatgcaggcg tatttgaatt tgaagaagat gagctgccgc
      241 tcttttcgca ggaaagtgta ttgccaaact gactaatttc agcagctgtt gcggtacgct
      301 ttaaacgcca cgggttagtt gcatcatttg ctttcgcttt tcccgggcaa tttgtagaaa
      361 aaccccacca ggcattttca taaattttgc tatcgaaaat aacctgatat ttattaccac
      421 cttgttggtt attccatgct tccattgcca atgctggcag agcggaacac accagcccca
      481 taccaatcat agatttagta aatatattta atttcattat aatcccttgt gacgtaaaaa
      541 ctgcaaaaca aaagtcctac gcaggaatta tttac
//
LOCUS       DAZOYL010000652          575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZOYL010000652 DAZOYL010000000
VERSION     DAZOYL010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.657"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>575)
                     /gene="ipaA"
                     /locus_tag="ACHUOY_004420"
     CDS             complement(<1..>575)
                     /gene="ipaA"
                     /locus_tag="ACHUOY_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR0684963.1"
                     /translation="YDNRVFDNRVYDNSYHENPENDAQSPTSQTNDLLSRNGNSLLNP
                     QRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDNSDGIGSDLLTTSSQERSAN
                     NSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKSSASLSHRVASQINKFNSNT
                     DSKVLQTDFFSRNGDTYLTRETIFEASKKVT"
BASE COUNT          145 a           88 c          133 g          209 t
ORIGIN      
        1 tgttactttt tttgacgctt caaatatcgt ttcccgtgtt aaatatgtgt ctccatttct
       61 tgagaaaaaa tcagtctgaa gtacttttga atcagtgttt gagttgaatt tattgatttg
      121 cgaagctact ctatgagaaa gagaagcgct acttttagtt gtatctgatg agttatcatt
      181 actgcttgtc actccttccg ggtggagagg gggggtggtt gaagagttct gtatgttcag
      241 aggcctgtgt ccccgagaaa gagagttatt tgcagatctt tcttgactac tcgtagttaa
      301 caggtctgaa cctattccat ccgaattatc tggagtatgg ttgaaaacct tttctaaagc
      361 tgaattattt gcaaatgtct ggacagtatc tgatatagag tgtggtagaa cggaagttac
      421 tttttgaact agtgctcttt gtggatttag taatgagttt ccgttacggg ataatagatc
      481 gtttgtctga gaagtaggag actgtgcatc attttctggg ttctcgtgat agctattgtc
      541 atatactctg ttgtcaaaaa ctctattatc gtaag
//
LOCUS       DAZOYL010000653          574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZOYL010000653 DAZOYL010000000
VERSION     DAZOYL010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.658"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..172
                     /locus_tag="ACHUOY_004421"
     CDS             <1..172
                     /locus_tag="ACHUOY_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684964.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..>574
                     /locus_tag="ACHUOY_004422"
     CDS             185..>574
                     /locus_tag="ACHUOY_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR0684965.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAA"
BASE COUNT          147 a          113 c          162 g          152 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcg
//
LOCUS       DAZOYL010000654          561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZOYL010000654 DAZOYL010000000
VERSION     DAZOYL010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.659"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            38..>561
                     /locus_tag="ACHUOY_004423"
     CDS             38..>561
                     /locus_tag="ACHUOY_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0684966.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
BASE COUNT          174 a          104 c          114 g          169 t
ORIGIN      
        1 caactttttg gaaacaatat tgtaagatca aatttatatg gtggcgaaaa ttaatctctt
       61 atcacggctt acgccgcttt tattcgtgtt cgctcctttt ttggcacagt caaatatgac
      121 tgtttatcct atggctgttt ccattaatag tcagggtgaa ggcaacgttc gcgtaatatc
      181 taaaagtaat gaagttcagt acatcaaggc gacagtattc cgtatcgata atccatcgac
      241 gcctcaggaa aatgaagttg agattaaatc cggtgatgca aatcatctgg ttgttatgcc
      301 acctaaattc gctttaccag ccggtagtag caaaactgtg cgctttgttg caatggaacc
      361 agagcaaaaa gaaaaaaatt atcgcgttaa atttgaagcg gttcccagta ttgatgacgt
      421 tgccacagat aaaaaagatc tctctatgca gttaacagtt aacttaattt gggggattgt
      481 tgttagtgtt ccacctcagc aacctattgc taagttagaa gtaaatgctg cccaaaaatt
      541 agttaatgca gggaatcaac g
//
LOCUS       DAZOYL010000655          559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZOYL010000655 DAZOYL010000000
VERSION     DAZOYL010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.660"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            33..494
                     /locus_tag="ACHUOY_004424"
     CDS             33..494
                     /locus_tag="ACHUOY_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0684967.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWMHRK"
BASE COUNT          160 a          114 c          145 g          140 t
ORIGIN      
        1 acaggtgcca tttatctgat atggagaata acatgaggaa atatattcca ctggtattat
       61 ttatcttttc atggccggta ttgtctgcag atattcatgg acgggttgtt cgtgtactgg
      121 acggtgatac catcgaggtc atggactcac tgaaggcggt gcggatcagg ctggtaaata
      181 ttgatgcacc ggaaaagaaa caggattatg gccggtggtc aacagacatg atgaaatcac
      241 tggtcgcagg aaaaacggtt accgtcacat attttcagcg agatcgttat ggtcgtattc
      301 tcgggcaggt ttatgcacca gacggaatga atattaatca gtttatggtc cgggccgggg
      361 ctgcctgggt atatgagcag tacaacacag atcccgtgct acctgtcctt cagaacgaag
      421 ccagacaaca aaaacgtggg ctctggtcag atgctgatcc ggttcccccc tggatatgga
      481 tgcaccgtaa ataaagcgat ggcaatccta tcattcatct gttctcagcg ggaataaaaa
      541 tggcaaaaac aaaacagga
//
LOCUS       DAZOYL010000656          559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZOYL010000656 DAZOYL010000000
VERSION     DAZOYL010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.661"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>559
                     /locus_tag="ACHUOY_004425"
     CDS             <1..>559
                     /locus_tag="ACHUOY_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684968.1"
                     /translation="SSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVNMLNDFSQIDND
                     LGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNTTNTFIFTIIPD
                     NNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLSIIPENFFTLLS
                     GNFKADWRWDVSKETFPKDKFDSYVK"
BASE COUNT          199 a           89 c           91 g          180 t
ORIGIN      
        1 ctcttcaaac catcaagata agcattcaga taaagttaac agtcatcaac accatggcaa
       61 ggttgataaa aaatatcgat caaacacagt tgaagttaat atgctgaatg acttttcgca
      121 aatagataac gatttaggcc tgaatatcat ctatttcctg caacatggtc actggagggt
      181 aaatgaacat agtcttcaga tggagaaaat atggttttac aatagtgagc catctataga
      241 tattcaagaa tataatagtt tcgccgataa tactactaac acatttatct ttacaattat
      301 accagacaat aaccatgtga taaaattatc gtctcccatt acagttaaga ttgagtgtaa
      361 ggatggttat tattttatta atttctccgg agataaatca gacataattt ataaatcaga
      421 cataacttat aaagttaatg ggctttctat catacctgaa aatttcttta ctcttcttag
      481 cggtaatttt aaagctgact ggcgttggga tgtctctaaa gagacgtttc ctaaggataa
      541 gtttgatagc tatgttaaa
//
LOCUS       DAZOYL010000657          558 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZOYL010000657 DAZOYL010000000
VERSION     DAZOYL010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.662"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            33..395
                     /locus_tag="ACHUOY_004426"
     CDS             33..395
                     /locus_tag="ACHUOY_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR0684969.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT          164 a          134 c          136 g          124 t
ORIGIN      
        1 gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca
       61 aacgtctcag cgatcgtctg gacgccatcc gccaccagca ggccgatctg tcgctggttg
      121 aagccgcaga caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg
      181 ctcgcctgcg tgaagttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga
      241 tgccgtttca gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa
      301 gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct gggccgtgaa atgggcctgc
      361 aggagatgac tggattctct aagactgcgt tttaagcgcc tcagacgact ggttcgatca
      421 tatcaacccg catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc
      481 ctaattacca aacaattcac tccctttacg tcgatttacc gctggttgag attctgaaaa
      541 tgcattttta ccgaaata
//
LOCUS       DAZOYL010000658          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZOYL010000658 DAZOYL010000000
VERSION     DAZOYL010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.663"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>557)
                     /locus_tag="ACHUOY_004427"
     CDS             complement(<1..>557)
                     /locus_tag="ACHUOY_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0684970.1"
                     /translation="FGFGCAFLPEDVIRFDTKSDFLAWVRNALPGEYSVAGPYDIIIP
                     DTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGINGPIYHTRYCYWPISRLTGWVKIN
                     ITTEDIIYRIVASSVCNRWGDPDIGGLIIAAYQGEADGDKVIRLVRGQSYRGSRLGPV
                     GISVPSTPTGTYIASPQFFITGCSE"
BASE COUNT          136 a          145 c          138 g          138 t
ORIGIN      
        1 ctctgaacat cccgtaatga aaaattgtgg ggatgctata tacgttccgg tgggagtact
       61 gggcactgaa atccccaccg gtcccagtcg tgagcctctg tatgactgcc ccctgacaag
      121 tctgatgact ttatcaccgt cagcttctcc ctggtacgca gcaataatca gcccgccaat
      181 gtcagggtct ccccatctgt tgcagacaga gctcgccacg attctgtaaa taatatcttc
      241 tgtggttata tttattttca cccagccagt cagtctggat atgggccagt agcagtagcg
      301 ggtgtgataa atggggccgt taatgccgta aaaagtaagg gatttggctc tgtacctcgg
      361 ttctgttgtc tcagggcgtg catcagtcca ccggatgctg agcacccctt caaaccgtgt
      421 gtcgggtatg atgatgtcgt aggggccagc aacggaatat tcacctggca gcgcattcct
      481 tacccaggcc aggaaatcac tcttagtgtc aaaacggata acatcttcag gcagaaaagc
      541 acacccaaag ccgaatg
//
LOCUS       DAZOYL010000659          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZOYL010000659 DAZOYL010000000
VERSION     DAZOYL010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.664"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUOY_004428"
     CDS             <1..>557
                     /locus_tag="ACHUOY_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0684971.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLK"
BASE COUNT          115 a          160 c          157 g          125 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAZOYL010000660          555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZOYL010000660 DAZOYL010000000
VERSION     DAZOYL010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.665"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..525)
                     /gene="fes"
                     /locus_tag="ACHUOY_004429"
     CDS             complement(<1..525)
                     /gene="fes"
                     /locus_tag="ACHUOY_004429"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR0684972.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERL"
BASE COUNT          115 a          153 c          135 g          152 t
ORIGIN      
        1 caaccgttca cttttccaga taatttcttt ggctgctatt tctggcactt gcggacaatc
       61 ccatcccggt tgcagaggcg cttgcggcat ttcgagtgca gaaacagcgt gccctcgccc
      121 gcctttccag ctttgtggat tcagcggatc ggctattgcc tggggtaata gttttcgcca
      181 gccttcacgt aattcgaggc gatcggggga tggtgcagaa aaaatgtcat cgcgttcggt
      241 gggaataaag cagtagctgc cgcgccagtt ggcattgagt tgtgtcgtcc actgccagac
      301 atcagtacct gcaattcgct gcatcgactg gggctggctg ttctgatgat gatcggtcac
      361 accagtgatg tagatccata cgcgctttat cgtcgagtat tcttcagaac cttggggatc
      421 acgccaccag aaagtgacct caaacatttc gtcattcaga cgctgccatt ccgggccatg
      481 tttcgactgc caccagctct cacttcctac ttttaacgcc gtcacaccat aaccccatgt
      541 ttactgtgca atttt
//
LOCUS       DAZOYL010000661          555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZOYL010000661 DAZOYL010000000
VERSION     DAZOYL010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.666"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          165 a          106 c          105 g          179 t
ORIGIN      
        1 ctgagtataa aatctcttct gatgtttaat tgatttgaat gttcgtaagc tatatcactt
       61 actcaatcca ttttacccag agtcataaaa tagagtgtct gttgatctca ttttcatatt
      121 cctttcagac tgaagtgcgt tttcatcacc cgatgagaaa gaactgaagt gatgtttgcc
      181 atagattgcg agatgccctg tctcattgac tatcgcggtg cacctttata cctgttatac
      241 cagatcaaaa atcacgcaag ccatacaaca aaacgagatt tgcaattcgt gtcacaaaat
      301 atgtcgatct ttttctaaga ggaagatgcc atgtgaagcc agacgaacac ttgaggtggt
      361 cttcaaaaac taaaggattt cttttaactg tttgttttat aaaaaattta tttagggtct
      421 aaacaaggga agctttgcaa gctaactcag tgagcgtggt gaaaacaagt gtttacccgc
      481 catcaggctg atcataattc tcaccatgaa atatgtttcc tgatttgtgg cttgtaactg
      541 gtcgcttctg aagtc
//
LOCUS       DAZOYL010000662          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZOYL010000662 DAZOYL010000000
VERSION     DAZOYL010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.667"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..111)
                     /locus_tag="ACHUOY_004430"
     CDS             complement(<1..111)
                     /locus_tag="ACHUOY_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684973.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFA"
     gene            complement(234..>553)
                     /gene="tabA"
                     /locus_tag="ACHUOY_004431"
     CDS             complement(234..>553)
                     /gene="tabA"
                     /locus_tag="ACHUOY_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0684974.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTFILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          161 a          132 c          104 g          156 t
ORIGIN      
        1 ggcaaaatta ttcattccaa cacgccaggt atttaaaact tcactttttt gtttaaaatt
       61 gtcggctaat gtaatgtgtt gagaataatt caaatctgat attttgctca tgacgaactc
      121 cttgagttaa taagaacgtc taagcattac aaattttact cacaaaaaaa tatcaaaaaa
      181 cgctcttttc acaaactcgc cgtaaacctg gagttattta cggcgaaaag tcatcacgcc
      241 atcagcatct tcactactgc tttgcgaacc tgggctggtg cacccactgc gcacagcggt
      301 ttatgcactt cccccggata aaacacaaca aaatcacctt cattaaggat aaatgttttc
      361 tcatcaacgc cttccggcaa aaatgcgatg tctttatcag ctaaccagtc ggtatccggc
      421 gtgcctgcag gttgcgtgct gaaggtcatg ccttcctgac cttttaacac aatctgaatg
      481 tcgagatagc gggcatggta ctccgtacgg cgagcttcgt gcggctcggt catatcttcc
      541 gagataagat aaa
//
LOCUS       DAZOYL010000663          550 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZOYL010000663 DAZOYL010000000
VERSION     DAZOYL010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.668"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>550
                     /gene="feaR"
                     /locus_tag="ACHUOY_004432"
     CDS             <1..>550
                     /gene="feaR"
                     /locus_tag="ACHUOY_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR0684975.1"
                     /translation="QEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDASR
                     PCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNNP
                     ALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIA
                     GETGMSVRSLYRMFADKGLVVA"
BASE COUNT          139 a          125 c          146 g          140 t
ORIGIN      
        1 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
       61 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
      121 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
      181 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
      241 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
      301 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
      361 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg ccgtgaacgt cagtttcaaa
      421 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
      481 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
      541 tagtcgcgca
//
LOCUS       DAZOYL010000664          548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZOYL010000664 DAZOYL010000000
VERSION     DAZOYL010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.669"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(86..162)
                     /locus_tag="ACHUOY_004433"
     tRNA            complement(86..162)
                     /locus_tag="ACHUOY_004433"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Arg,seq:tct)
     gene            complement(263..338)
                     /locus_tag="ACHUOY_004434"
     tRNA            complement(263..338)
                     /locus_tag="ACHUOY_004434"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(303..305),aa:Ile,seq:cat)
     gene            complement(441..>548)
                     /locus_tag="ACHUOY_004435"
     CDS             complement(441..>548)
                     /locus_tag="ACHUOY_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0684976.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          152 a          141 c          122 g          133 t
ORIGIN      
        1 caaacataaa atattttcaa caatatcctg agccaggtat atttcagtat aaggctctgc
       61 cggaaggaat ctggaagaat gaatatggcg cgctgtactg gattcgaacc agtgaccgat
      121 tgcttagaag gcaattgctc tgtccggctg agctaacaac gctgaatacc gataatggac
      181 cgccatcggg gacccgcccc cgcaccaaca accctgttat cgtgtcgtct gctcttcctg
      241 ataagctaat ggcggtttgt gatggtggcc cttgctggat ttgaaccagc gacttggcga
      301 ttatgagtcg ctcgctctca ccactgagct aaagggccgg gagcagaata ataacggtcc
      361 gtaattaatt ccgcaataaa aaacccgctc ggcggcgggt tgtagaaact cttctaacgt
      421 caggcataaa acgcccatag ttatgacgaa tttaccacag attccggaaa aatcaaccta
      481 gttacctttt ttaactgccg ctcagcccat gcttcttcaa tatcaaaccg agtcaccagc
      541 gcatcata
//
LOCUS       DAZOYL010000665          547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZOYL010000665 DAZOYL010000000
VERSION     DAZOYL010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.670"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            31..105
                     /locus_tag="ACHUOY_004436"
     CDS             31..105
                     /locus_tag="ACHUOY_004436"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684977.1"
                     /translation="MLRERTKSGLAAARQDGLVDGDQS"
     gene            277..>547
                     /locus_tag="ACHUOY_004437"
     CDS             277..>547
                     /locus_tag="ACHUOY_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0684978.1"
                     /translation="MVHKSDSDELSALRAENARIIKPLLLPEPATPRAGRPWAEHRKI
                     INGMFWVLCSGAPWRDLPERYGSWKTVYNRFNRWSKSGVINIIFNR"
BASE COUNT          139 a          139 c          143 g          126 t
ORIGIN      
        1 gttggttcct ttgctgaatt tgaacgcgcc atgcttcgtg aacggacaaa gagtggactt
       61 gcagcggcac gccaggacgg gttggtggat ggcgaccaaa gttaacaccc cagcaacaaa
      121 aagagattgt ctcgctcgtc acatcggggc agaaaacagg tgctgatgcc gcccgtctgt
      181 ttcgggttca cccttccacc gtggtgcgtc ttctcgccag acaccggatg actgagatca
      241 aacagacata atctatgcgg agatttacag cagggcatgg ttcacaaatc tgacagcgat
      301 gaattatcgg cactgcgggc agaaaatgct cgaatcatca agcccctgtt acttcctgaa
      361 cccgccacac cacgggccgg acgcccatgg gctgagcacc gtaaaatcat caatggcatg
      421 ttctgggtgt tatgttccgg tgcaccatgg cgcgatttac ccgaacgata tgggtcatgg
      481 aaaactgttt ataaccgctt taaccgatgg tcaaagtcag gcgtgattaa tattattttc
      541 aacaggt
//
LOCUS       DAZOYL010000666          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZOYL010000666 DAZOYL010000000
VERSION     DAZOYL010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.671"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHUOY_004438"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHUOY_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZOYL010000667          545 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZOYL010000667 DAZOYL010000000
VERSION     DAZOYL010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.672"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          192 a           81 c          112 g          160 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttg
//
LOCUS       DAZOYL010000668          544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZOYL010000668 DAZOYL010000000
VERSION     DAZOYL010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.673"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            241..>544
                     /locus_tag="ACHUOY_004439"
     CDS             241..>544
                     /locus_tag="ACHUOY_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR0684979.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          124 a          136 c          130 g          154 t
ORIGIN      
        1 tgtaatgtac gagcctgtcg tgaataacgt cgagaaccca tccgttgcgt ttgtgattat
       61 tttgttgact aaacagacac ccgtttctct gaagtaaaat cccagactaa atcatcacat
      121 aaccatgaca tttttctgat attccccggt aacgccagat gtcgactcgc ttatctcatc
      181 cgcccgacat gttgtacatg caatatgtgc ggattattat tcattttcac ggaggttgct
      241 atgtcaaccg gattacgttt cacactggaa gtggacggcc tgccaccgga tgcttttgcg
      301 gtggtttcct ttcatttgaa ccagtcactc tcttcgctct tttccctcga tctctctctg
      361 gtcagccagc agtttctctc ccttgaattt gcgcaggtgc tggacaaaat ggcctacctg
      421 acggtatggc agggcgatga cgtacagcgc cgggtgaaag gtgtggtgac ctggtttgaa
      481 ctgggggaga acgacaaaaa ccagatgctg tacagcatga aggtatgccc accgctgtgg
      541 cgca
//
LOCUS       DAZOYL010000669          542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZOYL010000669 DAZOYL010000000
VERSION     DAZOYL010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.674"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            39..188
                     /locus_tag="ACHUOY_004440"
                     /pseudo
     CDS             39..188
                     /locus_tag="ACHUOY_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            215..319
                     /locus_tag="ACHUOY_004441"
                     /pseudo
     CDS             215..319
                     /locus_tag="ACHUOY_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          183 a          106 c          110 g          143 t
ORIGIN      
        1 gtggacacgc ggctaagtga gtaaactctc agtcagaggt gactcacaag acaaaacagt
       61 ttcaaccagt aaaaaaacgt aaacaccatt cgcatgaatt tcgtagttaa gatctgaagc
      121 ttgcagaatg catcggtgtt actgccggag tccgtgaact tagtctgcat gaatcaccac
      181 agctttacat ctgtcgcagt aaacagcaaa atcagcaaac ttacttaatt atattgagtg
      241 cgattacaat cgctggcgat ggtacagttg gtgtggcggt ctcagtccgg aacaatttga
      301 aaatcagaat ctcacttaaa gcggcatcca ttttacaaaa gatggccgtc tgtgtatttg
      361 taatgaatac cttctacatg tttagagtgc ataatccacg acaaggcgat atcctaaaaa
      421 ttagctagca ggcaactttg atggcataga taatgttttt agtaaaaaag agttgactga
      481 atataataca gcctcttcct acataagaag gaatgtgctc acaaaaaaaa accattagtc
      541 tg
//
LOCUS       DAZOYL010000670          540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZOYL010000670 DAZOYL010000000
VERSION     DAZOYL010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.675"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>540
                     /locus_tag="ACHUOY_004442"
     CDS             <1..>540
                     /locus_tag="ACHUOY_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0684980.1"
                     /translation="PVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGS
                     LGPGWKMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKL
                     DEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYR
                     VLTGLVDRFGRTQTFHREA"
BASE COUNT           96 a          176 c          173 g           95 t
ORIGIN      
        1 atccggtcaa tcccctgctc ggtgcaaagg tccttcccgg tgaaaccgac atcgccctgc
       61 ccgccccgct gccgttcatc ctctcccgca cctacagcag ttaccggaca aaaacgcccg
      121 cgccggtggg gagcctcggc cccggctgga aaatgcctgc ggatatccgc ttacagctgc
      181 gcgataacac actgatactc agtgataacg gcggcagaag cctgtatttt gagcacctgt
      241 ttcccggtga ggacggttac agccgcagcg aatcactctg gctggtgcgc ggcggcgtgg
      301 cgaaactgga tgaaggtcac cggctggccg cactctggca ggcgctgccg gaagaactcc
      361 gcttaagtcc gcatcgttat ctggcgacaa acagtccgca ggggccgtgg tggctgctcg
      421 gctggtgtga gcgggtgccg gaagcggatg aggtgctgcc tgcgccgctg ccgccgtacc
      481 gggtactgac cgggctggtg gaccgcttcg ggcgcacaca gacgttccac cgcgaagccg
//
LOCUS       DAZOYL010000671          540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZOYL010000671 DAZOYL010000000
VERSION     DAZOYL010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.676"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>540
                     /locus_tag="ACHUOY_004443"
     CDS             <1..>540
                     /locus_tag="ACHUOY_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0684981.1"
                     /translation="ITTHPSGIKDTLAWLVNASKLMTEFDDNGKIIFNINALKYTKAS
                     YFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALSESIKGVKFCIW
                     FSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFACVSFGFPVI
                     LVGGAILLTGIVCTVVLNE"
BASE COUNT          159 a           90 c          117 g          174 t
ORIGIN      
        1 tatcactaca catccttcag gtataaaaga tacactcgcc tggttagtta acgcgagtaa
       61 attaatgact gagtttgatg acaatgggaa aattatattt aacattaatg ccttaaagta
      121 tacaaaagca agttactttg aaatacttgg agagaaatat ataaaaatca caacatccag
      181 cccctggctt ttagaaaagc tcggtaaata tattttctct tcacgagcgc ctcaagttct
      241 ggaactcgct attggctggc gaggagcttt atctgaatca ataaaaggag ttaagttctg
      301 tatctggttt tcagtagcct ggagaaccat agaatttatt atgagttcag agcgagatct
      361 ggttaatttt cttggcgatt tttcaatgga tgtggccaaa gcagttatag ccggtggtgt
      421 tgcaaccgct attggaagcc tggcttcttt tgcctgtgtt agctttggct ttccagtaat
      481 tcttgtcgga ggagcaattt tactgacagg gatagtgtgt acagttgttt taaatgaaat
//
LOCUS       DAZOYL010000672          539 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZOYL010000672 DAZOYL010000000
VERSION     DAZOYL010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.677"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>539
                     /locus_tag="ACHUOY_004444"
     CDS             <1..>539
                     /locus_tag="ACHUOY_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0684982.1"
                     /translation="KYTDHLPLYRQQHIFSRMGVELPVSTMADMVGVAGAALAPLVKL
                     LRHELLTRDVIHADETSLRLLDTRKGGKSCSGWLCAYVSGERSGPPVVCFDSQTGRAL
                     RYPETWLQCWCGGTLVSDGYSVYKSLADNHPGITSACCWSHAGRGFANLYKASREPRA
                     GVELRKIAGLYRIEKLIRE"
BASE COUNT          111 a          148 c          164 g          116 t
ORIGIN      
        1 gcaaatatac cgaccatctg cccctgtatc gccagcagca cattttctcc cgaatgggtg
       61 tggagctccc cgtcagtaca atggcggata tggtcggtgt tgccggagct gcactggccc
      121 cgctggtgaa actgctacgc catgagcttc tgacacggga tgttattcat gcagatgaaa
      181 cgtccctgag gctccttgat acccggaaag gcggtaaatc ctgctccggc tggctgtgtg
      241 cttacgtcag cggtgaaaga agcggtccgc cggtcgtctg ttttgactcg cagaccggac
      301 gggccctcag ataccctgag acctggctgc agtgctggtg tggcggtacg ctggtgagtg
      361 acggatacag cgtgtacaaa tctctggcgg ataatcaccc cggcatcaca tcagcctgtt
      421 gctggagtca cgcagggcgc ggcttcgcaa atctgtataa ggccagcagg gagccccgtg
      481 caggcgtgga gctgagaaaa atcgcgggtc tttaccgcat tgagaaactc atccgtgag
//
LOCUS       DAZOYL010000673          533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZOYL010000673 DAZOYL010000000
VERSION     DAZOYL010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.678"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>533)
                     /locus_tag="ACHUOY_004445"
     CDS             complement(<1..>533)
                     /locus_tag="ACHUOY_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0684983.1"
                     /translation="WFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDN
                     ALYLNPQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLR
                     KFEPFLEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQ
                     LGNNSYRDTQGILGVKY"
BASE COUNT          139 a          125 c           91 g          178 t
ORIGIN      
        1 atatttcaca cccaaaatac cttgggtgtc tctgtaactg ttattaccca attgctgaga
       61 tacgtttccc cagatgctta ggttatctgc aagacgcccc cccacaccag ttttgagttc
      121 aatcacatta cggttgccta tctggtgatt tgacatgcca ttcataatta caccatattg
      181 ctttgagctc catttccaat tcacttccaa aaatggttcg aacttacgaa gagtctcctt
      241 atcgatacaa ctctgactaa ttaaggatgt acgcaatccc aattttgctt gaatattatt
      301 gaccccagag gatgtgacca tagtgccatt gtgttcaata tagtcgtttg cttttacccc
      361 atgcctagtt atctggactt gtggattgag atacaatgcg ttgtcaatat tattatgagc
      421 agtccagcgt atggttgggt agatatagcc agcttccaaa gctcctgtta ttcctttgct
      481 ggaatatttc tcaccagtta acccatctcc tttgactgta ttattaaacc aac
//
LOCUS       DAZOYL010000674          532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZOYL010000674 DAZOYL010000000
VERSION     DAZOYL010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.679"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            66..>532
                     /gene="dosC"
                     /locus_tag="ACHUOY_004446"
     CDS             66..>532
                     /gene="dosC"
                     /locus_tag="ACHUOY_004446"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR0684984.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFT"
BASE COUNT          140 a          111 c          132 g          149 t
ORIGIN      
        1 ttagtcaatc tcgcacgttg cgttttgatt ccgtctgatt tcccagcctt ataagggtgt
       61 gaattatgga gatgtatttt aaaagaatga aagatgagtg gaccggactt gtcgaacagg
      121 cagatccact cattcgtgct aaagccgcgg aaattgccgt tgcgcatgct cattatctga
      181 gtatcgagtt ttatcgaatt gtccgcatcg acccgcatgc cgaagaattc ttgagtaatg
      241 aacaagttga gcggcagttg aaaagtgcga tggaacgctg gattattaac gtgctttctg
      301 cccaggttga cgatgtcgaa aggctaatac aaatccagca taccgtcgcg gaagtgcatg
      361 cccgcatagg aattccggta gaaattgtcg agatggggtt tcgggtgctg aaaaaaatcc
      421 tctatccggt aatcttctct tcggattatt ccgccgcaga aaaacttcag gtctatcatt
      481 tctcgattaa cagtattgat atcgcgatgg aagtgatgac ccgcgcgttt ac
//
LOCUS       DAZOYL010000675          531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZOYL010000675 DAZOYL010000000
VERSION     DAZOYL010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.680"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            84..>531
                     /locus_tag="ACHUOY_004447"
     CDS             84..>531
                     /locus_tag="ACHUOY_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR0684985.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT          117 a          117 c          134 g          163 t
ORIGIN      
        1 tatgcgaaaa tgcagatggc aatgagatcc actgctttca tctccattaa catcccatta
       61 cgcttttatt aaggagcatt agcatgttct caccgcagtc acgcttgcgt catgcagttg
      121 cagatacgtt cgcgatggtt gtttactgtt ctgtcgtgaa catgtgtatt gaagttttcc
      181 tctccggaat gagcttcgaa cagtcttttt attccagatt ggtagcgatt ccggtgaaca
      241 tcttaattgc atggccatac ggtatgtacc gcgatctatt tatgcgcgcg gcacgcaaag
      301 ttagcccgtc gggctggata aaaaatctgg ctgatatcct ggcttatgtg acgttccagt
      361 caccggtgta tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag attatggcgg
      421 cggtcagttc aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc tacttcctcg
      481 attattgccg ccgactgttt aaagtcagcc attaccagca ggtaaaagcc t
//
LOCUS       DAZOYL010000676          520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZOYL010000676 DAZOYL010000000
VERSION     DAZOYL010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.681"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>520
                     /locus_tag="ACHUOY_004448"
     CDS             <1..>520
                     /locus_tag="ACHUOY_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0684986.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAZOYL010000677          515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZOYL010000677 DAZOYL010000000
VERSION     DAZOYL010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.682"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>515
                     /locus_tag="ACHUOY_004449"
     CDS             <1..>515
                     /locus_tag="ACHUOY_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0684987.1"
                     /translation="RMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNLLPTNDIDQLIS
                     SKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTTQPPGLQNFTSI
                     NLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMNNNALRNIDIDR
                     LSSVTYFSAAH"
BASE COUNT          199 a          103 c           69 g          144 t
ORIGIN      
        1 caggatgaaa acaatcactt taaacgacaa ccatattgca catttaaacg ccaaaaacac
       61 tacaaaactg gaatatttaa acttaagcaa taacaattta ctgccaacca atgacattga
      121 tcaactaata tcatcaaaac atctttggca tgtattagtt aacggcatca acaatgatcc
      181 acttgcacaa atgcagtact ggactgcagt aagaaatata attgatgaca ctaatgaagt
      241 gaccattgat ttatcaggac ttaatttaac cactcaacca ccagggctgc aaaacttcac
      301 atctatcaat cttgataata accaactcac acattttgat gcaaccaact acgatagact
      361 cgtaaagcta agtctgaata gtaatgctct tgagtcaata aattttcctc aaggcagaaa
      421 tgtaagtatt acacatatat ctatgaataa taatgctctc agaaatattg atatagatag
      481 gctttcatca gttacttatt ttagtgcggc acata
//
LOCUS       DAZOYL010000678          507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZOYL010000678 DAZOYL010000000
VERSION     DAZOYL010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.683"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..188)
                     /locus_tag="ACHUOY_004450"
     CDS             complement(<1..188)
                     /locus_tag="ACHUOY_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0684988.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="ACHUOY_004451"
                     /pseudo
     CDS             242..445
                     /locus_tag="ACHUOY_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          126 a          136 c          117 g          128 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggattcc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaac
//
LOCUS       DAZOYL010000679          506 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZOYL010000679 DAZOYL010000000
VERSION     DAZOYL010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.684"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            32..>506
                     /gene="icsP"
                     /locus_tag="ACHUOY_004452"
     CDS             32..>506
                     /gene="icsP"
                     /locus_tag="ACHUOY_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR0684989.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESR"
BASE COUNT          162 a          100 c          109 g          135 t
ORIGIN      
        1 ggacatttca accaaaaaag tagagttctc gatgaaatta aaattccttg tacttgcact
       61 ttgtgtacca gcgatcttta ctacacatgc taccactaac tatccacttt tcataccgga
      121 caacatcagt acggatatta gtctaggatc tctgagtggc aaaacaaaag aacgcgttta
      181 tcatcccaag gaagggggga gaaaaattag tcaactggac tggaaataca gtaatgcgac
      241 tattgttaga ggtggcatcg attggaagct aattccaaaa gtgtctttcg gagtttccgg
      301 ttggactact ttaggtaacc agaaagcaag catggttgat aaagactgga acaattccaa
      361 tacccctcag gtatggaccg accagagctg gcatcccaat acgcatctcc gtgatgctaa
      421 cgaattcgag ctgaatctta aaggttggtt attaaataat ttggattatc gacttggact
      481 aatagcaggt taccaagaga gtcgtt
//
LOCUS       DAZOYL010000680          501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZOYL010000680 DAZOYL010000000
VERSION     DAZOYL010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.685"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>501
                     /locus_tag="ACHUOY_004453"
     CDS             <1..>501
                     /locus_tag="ACHUOY_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR0684990.1"
                     /translation="SFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSA
                     LTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQ
                     EQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRI
                     NGKDHDL"
BASE COUNT          144 a           95 c          110 g          152 t
ORIGIN      
        1 tcatttattg gtcttttttt catcattaac tatcaactgg tatcggaacg cgcggtaaaa
       61 cgtgccgata gccgctttga acttattcag aaaaacgttg gctatttctt taaagatatt
      121 gaacgttcgg ccctgacatt aaaggactca ctgtatttat taaaaaatac agaggagatt
      181 caacgcgccg tgattctgaa aatggaaatg atgccatttt tagactcggt gggactggta
      241 cttgatgata ataaatatta tctcttttcg cggagggcga atgataaaat cgttgtttat
      301 catcaggaac aagtaaatgg accgcttgtc gacgagtcag ggcgggttat ttttgccgat
      361 tttaacccat cgaaacgacc gtggtcggtg gcttcagatg actctaacaa cagctggaat
      421 ccggcataca attgctttga tcgtccgggt aaaaaatgta tctcttttac gctacgtatc
      481 aacggcaaag atcacgattt g
//
LOCUS       DAZOYL010000681          499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZOYL010000681 DAZOYL010000000
VERSION     DAZOYL010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.686"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>499)
                     /locus_tag="ACHUOY_004454"
     CDS             complement(<1..>499)
                     /locus_tag="ACHUOY_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
                     /protein_id="HFR0684991.1"
                     /translation="VSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSR
                     AVIGWSMSPRLTAQLACDALQMALWRRKRPRNVIVHSDRGSQYCSADYQALLKWHNLR
                     GSMSAKGCCYDNACVESFFHSLKVECLHGEHFISREIMRATVFNYIECDYNRWRRHSW
                     CGGLSP"
BASE COUNT          118 a          153 c          116 g          112 t
ORIGIN      
        1 ccggactgag gccgccacac caactgtgcc gtcgccagcg attgtaatca cattcgatat
       61 aattaaacac tgttgcccgc attatttccc ggctgataaa gtgttctccg tggagacatt
      121 ccactttcag cgaatgaaag aagctttcca cgcaggcatt atcgtagcag cagccctttg
      181 cgctcatact gccacgcaga ttatgccact tcagcagcgc ctgataatct gctgaacagt
      241 actgacttcc tcggtccgag tgaacgataa cgttccgggg tctcttacgc cgccacagcg
      301 ccatctgcag ggcatcgcag gccagttgcg ccgtcaggcg tggtgacatg gaccagccaa
      361 taacggcacg tgaccacagg tcaatgacca cagccagata cagccagcct tcatctgtac
      421 gtaagtacgt gatgtctccc gcccacttct ggttcggacc actggcgtaa aaatcctgct
      481 ccagcagatt ttctgacac
//
LOCUS       DAZOYL010000682          497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZOYL010000682 DAZOYL010000000
VERSION     DAZOYL010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.687"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>497
                     /gene="virK"
                     /locus_tag="ACHUOY_004455"
     CDS             <1..>497
                     /gene="virK"
                     /locus_tag="ACHUOY_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR0684992.1"
                     /translation="DIQCSPSGFDREGELMLSLFFNKIVIARLTFSVILTQNGHTAFI
                     GGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVSECLAVSEHSHVFR
                     QLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSNIASKKRSEYRKRY
                     ALLDY"
BASE COUNT          131 a           95 c          121 g          150 t
ORIGIN      
        1 ttgatatcca gtgttctccc agtggttttg accgggaagg agagttgatg ttgtcattgt
       61 ttttcaataa aatagtgatt gcccggttaa cattttcagt cattctgact caaaatggac
      121 acaccgcttt tattggcgga ttacagggag cccccaaaaa taccggacct gatgttattc
      181 ggtgcgcaac ccgggcctgt tatgggcttt tcccaaaacg gattattttt gaagcatttt
      241 gtgcgttgat gaaagcttgt aatgtctctg agtgtctggc agtcagtgag cacagtcatg
      301 ttttccggca attgagatac tggtatcaga aacgcaaaac ctttgttgct gtctacagtg
      361 atttctggga gtctgtagca gggaaaacct gtggtgactg gtacaaatta ccaacacagg
      421 tggttcgaaa gccgctaagc aatatagcta gtaaaaaacg ctctgagtat cgaaaacgat
      481 acgctttgtt ggattat
//
LOCUS       DAZOYL010000683          494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZOYL010000683 DAZOYL010000000
VERSION     DAZOYL010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.688"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            31..198
                     /locus_tag="ACHUOY_004456"
     CDS             31..198
                     /locus_tag="ACHUOY_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR0684993.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT          140 a          111 c           85 g          158 t
ORIGIN      
        1 taattaatat ataatacata gggaatgtaa atgaacaaat ttattaaagt tgcactggta
       61 ggtgcagtac tggctacgtt aactgcatgt actggtcata ttgaaaaccg tgataagaac
      121 tgctcttacg actacctgct gcacccggca atttctattt ctaaaatcat tggcggttgc
      181 ggtcctactg cacagtaagt ctctgacaga aatcggctaa caccggttta attgcccgac
      241 gccctctgga ttttccgagg gcgtatttgt ttctatgtcc ttcttgcccc cgcccgtaaa
      301 aatattttca ctttaaattc aatttgataa ctacatccat tagccacagc gcgatcctgt
      361 gcgaaatttt gtgatcttcc tccacattac ataacatcat atgttgttat attcatcatg
      421 cattgtcatg ttacctttta aatgactgca aactctcccc tacaacgtat tggacaagaa
      481 aaaggtatcg ctat
//
LOCUS       DAZOYL010000684          494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZOYL010000684 DAZOYL010000000
VERSION     DAZOYL010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.689"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          159 a          107 c          120 g          108 t
ORIGIN      
        1 tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat tatgcttcta
       61 tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag gaaacttggg
      121 gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat caaaccttat
      181 agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg acaggtaaga
      241 ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg gaataaggct
      301 caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg gatacagagc
      361 agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg cgttcataca
      421 ggtacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct gtcattactg
      481 acaggaaatc ctgc
//
LOCUS       DAZOYL010000685          490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZOYL010000685 DAZOYL010000000
VERSION     DAZOYL010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.690"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>490)
                     /gene="fimZ"
                     /locus_tag="ACHUOY_004457"
     CDS             complement(<1..>490)
                     /gene="fimZ"
                     /locus_tag="ACHUOY_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR0684994.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDY"
BASE COUNT          151 a           91 c           92 g          156 t
ORIGIN      
        1 cgtagtcgat aagctctaca attgaatgca aacctagttt gccataaata ttagatttat
       61 gcgcactaac tgttttattg ctaagtaata acttatcggc aatttcttta ttagataatc
      121 cgctaaccag ataacgtaat atggtcactt cacgattaga tagcacagtg accgttgaac
      181 tattcgtact acatttattg ctttttatat agttgagcgt ttcgctagga aaaaacgtgt
      241 atccggagag gatcatctga acgggatgaa aaatatcatt ctgatcattg catttactga
      301 caaaaccgtt agcaccagct tgtatcgctc tgccagcata aaagcattct gatttcgatg
      361 ataaaaataa cactttcact gtgctctgga tttgtttgat ccttttcagg aaggtaaaac
      421 cgtctgttcc gggcaagtct atatccataa tgattaaatc aacaggacgg gttcggagat
      481 aatcgatggt
//
LOCUS       DAZOYL010000686          483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZOYL010000686 DAZOYL010000000
VERSION     DAZOYL010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.691"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>483)
                     /locus_tag="ACHUOY_004458"
     CDS             complement(<1..>483)
                     /locus_tag="ACHUOY_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0684995.1"
                     /translation="YFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLFFN
                     LDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSILV
                     NDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGYV
                     "
BASE COUNT          168 a           90 c           58 g          167 t
ORIGIN      
        1 cgacataacc cggaagttta tttacaattt cttttgtttt ttcttcatca ccataaacat
       61 atcctaactc attaagatac agctgttcgc cctcataaca cttaagtaat tgatagaagt
      121 accacttagc aaaatcaggt tgcttcatga aaaaatcctt caaaaaaata tcatttacta
      181 aaatgctgac aggcacttca ttaaatagta acttaacatc atcaaataac cttagtatta
      241 agcgtgattt ttcttgattg cttaagctaa aatcattgac atatgcaacc aacttatccc
      301 aaatcaattc ctgtaattta cggctatttt tatagggttc tttatcgaga ttgaaaaata
      361 aggtctcagc tattttaatt ttagattgat cgcagctaaa atctttagat tgatcgcagc
      421 taaaatcaat atgatttatc acggagcatg catactttat cttttcagaa tcaccaaaat
      481 att
//
LOCUS       DAZOYL010000687          481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZOYL010000687 DAZOYL010000000
VERSION     DAZOYL010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.692"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..270
                     /gene="yacA"
                     /locus_tag="ACHUOY_004459"
     CDS             1..270
                     /gene="yacA"
                     /locus_tag="ACHUOY_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFR0684996.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            270..481
                     /locus_tag="ACHUOY_004460"
                     /pseudo
     CDS             270..481
                     /locus_tag="ACHUOY_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          124 a          106 c          144 g          107 t
ORIGIN      
        1 atggcacagg ttaatatgag tgtaagaatc gacgcagaac tgaaggatgc ttttatggcc
       61 gctgcaaaaa gcatggaccg taatggctct cagttaatcc gggattttat gcgccagacc
      121 gttgaacggc agcacaattc ttggttccgt gaccaggttg cggcaggccg tcagcaactc
      181 gaatgcggtg atgtgcttcc ccatgacatg gtcgaatcct ctgctgctgc atggcgggat
      241 gaaatgagca ggaagattgc tgataaatga tggagatgtt ctggaccatg ctggccatcc
      301 aggacaggaa gcgcattcgt agtacattgc ggagcagaac ctgatggccg ccattgaacc
      361 ggacgaacgg attggttatt cagccagcag cctggcaggg cagccgtata aaggccgcaa
      421 cggtcgtgtg gaaggaacat ctggaccaca caaggtagcg tgtaacgtta ttttgtgtga
      481 a
//
LOCUS       DAZOYL010000688          481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZOYL010000688 DAZOYL010000000
VERSION     DAZOYL010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.693"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>481
                     /locus_tag="ACHUOY_004461"
     CDS             <1..>481
                     /locus_tag="ACHUOY_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0684997.1"
                     /translation="LEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEI
                     MKFVMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFER
                     KDESSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITD
                     "
BASE COUNT          161 a           76 c          102 g          142 t
ORIGIN      
        1 tttggaattt ttggtaaaaa tatttactct aggttatgtg gattttagca aacgctccaa
       61 cgaagcggga agaaatatga tggctcatat tgagtcctca tcttatatca aaaataatga
      121 tggcagtgag ataatgaagt ttgttatgaa taatcctgaa ggggaacgag cggatttatc
      181 taaggtggaa atagaaatca cactttccgc tttcactact atggggactc gtcaaggaca
      241 tacggctatt atatttcaac aacctgatgg ttcgactaat cgttatgaag gaaagtcctt
      301 tgaaagaaaa gatgagagtt cattacacct gattactaac aagattctgg catgttacca
      361 acgcgaagct aacaaggaaa tagctcgtct attaaatatt ccgcaggagt taaataattc
      421 tcaggacttg aataactcgc aggtgagttg taaagattca gttgattcta cgattacgga
      481 t
//
LOCUS       DAZOYL010000689          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZOYL010000689 DAZOYL010000000
VERSION     DAZOYL010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.694"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHUOY_004462"
     rRNA            <1..>476
                     /locus_tag="ACHUOY_004462"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZOYL010000690          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZOYL010000690 DAZOYL010000000
VERSION     DAZOYL010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.695"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..421)
                     /gene="mxiC"
                     /locus_tag="ACHUOY_004463"
     CDS             complement(<1..421)
                     /gene="mxiC"
                     /locus_tag="ACHUOY_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR0684998.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVE"
BASE COUNT          142 a           99 c           61 g          170 t
ORIGIN      
        1 attcgacttg ttcagcagta agatcttttt cattcaaaag ttcccttaat gctaagactt
       61 gatcacttgg atctttaaaa tatctcttta gtctgtctat aaaatcccga tctagaggaa
      121 ggttatcttt taacgttctc tttaaatcaa aaatcttgtg attaatttca tcttcttccc
      181 catctaaaat acgttcctga ctatcactat ttgttccttt cagtttctca aggtctcttc
      241 tgtttataaa tgacgaaaga gcggaagcca tctcatcaga tgagtcaata tacttgctat
      301 tagccaagcc agaatcaagc tctgcatcag cagtctcatc tccatcatct gaatttgtca
      361 ttgcattcaa cttatcaatg aatgcagagc taaaaactcc tgtattttta acatcaagca
      421 tacatcacct actttatagt tagtaattta agtatgaaac caatgatttt tc
//
LOCUS       DAZOYL010000691          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZOYL010000691 DAZOYL010000000
VERSION     DAZOYL010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.696"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            89..448
                     /locus_tag="ACHUOY_004464"
                     /pseudo
     CDS             89..448
                     /locus_tag="ACHUOY_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAZOYL010000692          469 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZOYL010000692 DAZOYL010000000
VERSION     DAZOYL010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.697"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            17..>469
                     /gene="spy"
                     /locus_tag="ACHUOY_004465"
     CDS             17..>469
                     /gene="spy"
                     /locus_tag="ACHUOY_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR0684999.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
BASE COUNT          152 a          126 c          114 g           77 t
ORIGIN      
        1 aggaaggata tagaatatgc gtaaattaac tgcactgttt gttgcctcta ccctggctct
       61 tggcgcggct aacctggccc atgccgcaga caccactacc gcagcaccgg ctgacgcgaa
      121 gccgatgatg caccacaaag gcaagttcgg tccgcatcag gacatgatgt tcaaagacct
      181 gaacctgacc gacgcgcaga aacagcagat ccgcgaaatc atgaaaggcc agcgtgacca
      241 gatgaaacgt ccgccgctgg aagaacgccg cgcaatgcat gacatcattg ccagcgatac
      301 cttcgataaa gcaaaagctg aagcgcagat cgcaaaaatg gaagaacagc gcaaagctaa
      361 catgctggcg cacatggaaa cccagaacaa aatttacaac atcctgacgc cggaacagaa
      421 aaagcaattt aatgctaatt ttgagaagcg tctgacagaa cgtccagcg
//
LOCUS       DAZOYL010000693          461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZOYL010000693 DAZOYL010000000
VERSION     DAZOYL010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.698"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>461
                     /locus_tag="ACHUOY_004466"
     CDS             <1..>461
                     /locus_tag="ACHUOY_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0685000.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAZOYL010000694          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZOYL010000694 DAZOYL010000000
VERSION     DAZOYL010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.699"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(29..298)
                     /locus_tag="ACHUOY_004467"
     CDS             complement(29..298)
                     /locus_tag="ACHUOY_004467"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0685001.1"
                     /translation="MWCFFNLFGVLIPIDERNLTRERTQVGLQAARARGRKGGRPKTL
                     SKDKQALAVQLYNEKKHTVAQICVLMGISRPTLYKYIESARLFKK"
BASE COUNT          112 a          127 c           89 g          131 t
ORIGIN      
        1 cccaaccgta cgaaaacgta cgctaagttc atttcttgaa caacctggct gactctatgt
       61 atttgtacag cgttggcctc gatatcccca tcaacacaca aatctgcgca actgtatgtt
      121 ttttctcgtt atagagttga acagcaaggg cctgtttatc cttactcagt gttttcggcc
      181 tgccgccctt acgtcctctg gctcgtgctg cttgaagccc gacctgagtt ctctctcttg
      241 tcaggttgcg ttcatcgata ggaattaaaa ccccaaaaag attaaaaaaa caccacaaaa
      301 cggatgtttc ttcaacacca cttttgctcc atatgaacgg aaccgacgat taaactggat
      361 ggctctgatt gattcagggt atgaatggcg gttttttgct ccgtttccct caaaatggac
      421 gcaacttccc ctctgcggct ctcagccgca ccaccgcat
//
LOCUS       DAZOYL010000695          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZOYL010000695 DAZOYL010000000
VERSION     DAZOYL010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.700"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>459
                     /locus_tag="ACHUOY_004468"
     CDS             <1..>459
                     /locus_tag="ACHUOY_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR0685002.1"
                     /translation="YSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKA
                     YFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMS
                     ESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLILKGS"
BASE COUNT          149 a           84 c           94 g          132 t
ORIGIN      
        1 ttattcttca gctgaaaaat ttctcactca cttcaacaaa aaatgcaaaa accagacttt
       61 ggcgttgatt tccagccgtc ctgaggggcg ttgcgttgct gcctgcggtg atttcgggct
      121 agttatgaaa gcatattttg acaagatgga atcaaatggc atcagtgtta tggcagccat
      181 attactggtg gataaccatg ctttgacggt ccggctaaga ataaagaaca caactgaagg
      241 atgtacccat tacgtggttt cggtttatga tcctaatgta actaacgaca aaataagaat
      301 catgagcgaa agcaaagagg atattaaaca ctattctctg atggatttta tgaatgtaga
      361 ttatagcctc ctgaaatggt caaatgatca tgttattaac caatctgttg caataattcc
      421 agcacttccg aaagaacagc tattgatatt aaaaggatc
//
LOCUS       DAZOYL010000696          455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZOYL010000696 DAZOYL010000000
VERSION     DAZOYL010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.701"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..386
                     /locus_tag="ACHUOY_004469"
     CDS             <1..386
                     /locus_tag="ACHUOY_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0685003.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           96 c          122 g          104 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agcac
//
LOCUS       DAZOYL010000697          444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZOYL010000697 DAZOYL010000000
VERSION     DAZOYL010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.702"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>444
                     /locus_tag="ACHUOY_004470"
     CDS             <1..>444
                     /locus_tag="ACHUOY_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0685004.1"
                     /translation="NQGRLNKAFEKKFPLTELNNPAHDSSAMSEKSHGREEIRLHIVC
                     DVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSADLTAEKFATAI
                     RNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVL"
BASE COUNT          148 a           77 c          108 g          111 t
ORIGIN      
        1 gaaaccaggg gcggctaaat aaagcctttg agaaaaaatt tccgctgaca gaattaaata
       61 atccagcgca tgacagttcc gcaatgagtg aaaagagtca cggcagagaa gaaatccgtc
      121 ttcatattgt ttgcgatgtc cctgatgaac ttattgattt cacgtttgaa tggaaagggc
      181 tgaaaaaatt atgcgtggca gtctcctttc gctcaataat agcagagcaa aagaaagagc
      241 ccgaaatgat ggtcagatat tatatcagtt ctgctgattt aaccgctgag aagtttgcga
      301 cagcgattcg aaatcactgg cacgtggaga ataatctgca ctggcgtctg gacgtggtaa
      361 tgaatgaaga cgactgcaaa ataagaagag gaaatgcagc agaattattt tcagggatac
      421 ggcacattgc tattaatgtt ttga
//
LOCUS       DAZOYL010000698          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZOYL010000698 DAZOYL010000000
VERSION     DAZOYL010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.703"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>442
                     /locus_tag="ACHUOY_004471"
     CDS             <1..>442
                     /locus_tag="ACHUOY_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0685005.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAZOYL010000699          441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZOYL010000699 DAZOYL010000000
VERSION     DAZOYL010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.704"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            109..>441
                     /locus_tag="ACHUOY_004472"
     CDS             109..>441
                     /locus_tag="ACHUOY_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0685006.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLR"
BASE COUNT          110 a          103 c          113 g          115 t
ORIGIN      
        1 gagacagatg ctggccaacg tgggatattt aacgcgggtt aacatcgttg tcagagccgc
       61 atgggtgagc tgttgctaca ccccggtatt tccaccacgg aggccagcat gaacaacaat
      121 aacacattgt acgtcgggtt agatgttcac aaagaatcga ttactgtcgc ttatgctatc
      181 aattcagaac ctgttgaact gatgggtaaa attggcacat cacctactga tattcagaat
      241 ctttgtaaac gtctcaggtc aaagtcatcg caggtcagta tcgtttatga agcggggccc
      301 tgtggttatg gactttatcg tcggctggtg aaatccggtt ttgactgcat ggtctgcgcc
      361 ccctcactta tcccgaaaaa accgggggag cgggttaaaa ccgatcgccg tgatgccatc
      421 agacttgtgc gttcactgcg t
//
LOCUS       DAZOYL010000700          439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZOYL010000700 DAZOYL010000000
VERSION     DAZOYL010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.705"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          130 a           64 c           77 g          168 t
ORIGIN      
        1 cccggtgatc atataatttc aaatgtaatt ataagaaaat agtgtactga ttctccgcat
       61 taaacaagtg gttaatgtat gtaaaacatt tcattaacat taagttgatt gttgcctttc
      121 tttgtattta attagtttgt tgtgtggtaa gtgtctgttt aaaaatagct tatctggtat
      181 tgcatgaaag tgattattta tagcagatga ttatttacgg tgatttattt taactgtgcg
      241 caacatccca tttcgattat tcctgtttca tttttgcttg ctagcgtagc gaaaaacttt
      301 ttaacatatt gaaatacacc caaaacaaaa gtatgactta tacatttatg ttaagtaatt
      361 gagtgttttg tgtgatctgc atcacacatt attgaaaatc gcagcccccc tccgttgtat
      421 gtgcgtgtag tgacgagta
//
LOCUS       DAZOYL010000701          439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZOYL010000701 DAZOYL010000000
VERSION     DAZOYL010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.706"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..93
                     /locus_tag="ACHUOY_004473"
     CDS             <1..93
                     /locus_tag="ACHUOY_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="SpvC; OpsF from Shigella was shown to remove
                     phosphate from threonine groups; may inactivate mitogen
                     activated kinases during infection; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector phosphothreonine lyase"
                     /protein_id="HFR0685007.1"
                     /translation="YRNELRSDRDGSERQEQMLREEPFYRLMIE"
BASE COUNT          114 a           97 c           93 g          135 t
ORIGIN      
        1 tatcgtaatg aactacgaag cgatcgagat ggaagtgaaa ggcaagagca aatgttacga
       61 gaggaaccat tttatcgttt gatgatagag tagagaagat gccagctaca ccaccttgta
      121 gcttgaaaac tggttagaat aactacgatt agatttggca ctgcgtatgc agcctgattc
      181 ctgcccgtga cagagcattc ggctcataaa ctgctgagct actcatctga aatccgaaca
      241 gttgtccacc acttcagcgt gcatctcctg tttgagtcgt ttgattgctt ttcgggatat
      301 taacctgtcg gattttgcat tagttaatgc tcccatcagg ccttcatctt ttccagtgcc
      361 tattttcctg ttctcctttt gcacaatata atcagatcat ctgtatcgtt gacgagctcg
      421 ctatcttata ggagcattc
//
LOCUS       DAZOYL010000702          437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZOYL010000702 DAZOYL010000000
VERSION     DAZOYL010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.707"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..112)
                     /locus_tag="ACHUOY_004474"
     CDS             complement(<1..112)
                     /locus_tag="ACHUOY_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685008.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKF"
     gene            complement(368..>437)
                     /locus_tag="ACHUOY_004475"
     CDS             complement(368..>437)
                     /locus_tag="ACHUOY_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR0685009.1"
                     /translation="QLINVNFFSSDRTSFCECNRFP"
BASE COUNT          126 a           89 c           87 g          135 t
ORIGIN      
        1 caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta accactgcgt
       61 gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc attgatgcct
      121 gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcagggt tctttcgcac
      181 tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc tgtggcctac
      241 gacgatcaaa agctctgtga aaatttccct tgttgtcttt gtctgacctt atcgcggcga
      301 tcagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc tatgagggga
      361 gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt
      421 tgacgttaat caattgt
//
LOCUS       DAZOYL010000703          436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZOYL010000703 DAZOYL010000000
VERSION     DAZOYL010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.708"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>436
                     /locus_tag="ACHUOY_004476"
     CDS             <1..>436
                     /locus_tag="ACHUOY_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0685010.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEV"
BASE COUNT          127 a          113 c          122 g           74 t
ORIGIN      
        1 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
       61 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      121 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      181 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      241 atggataaag gttattactc actgggactg ttaaatgcct ggagcctggc gggagaacac
      301 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      361 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      421 ctgggaaatg aagtga
//
LOCUS       DAZOYL010000704          427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZOYL010000704 DAZOYL010000000
VERSION     DAZOYL010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.709"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..124)
                     /locus_tag="ACHUOY_004477"
     CDS             complement(<1..124)
                     /locus_tag="ACHUOY_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFR0685011.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
BASE COUNT          158 a           93 c           66 g          110 t
ORIGIN      
        1 tataaggggt tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg
       61 tcagcttttc cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg
      121 ccatggttct ttccaccatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga
      181 aaaaacacaa aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa
      241 actacaagac gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa
      301 ataattctgc tctatggttt acattcaaaa atatcattta tacttttcag aacatcacca
      361 gcaaggcata aacaaggaaa ctaaatgaag tggattgtga ttgatacagt tatccagcca
      421 tcatgcg
//
LOCUS       DAZOYL010000705          424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZOYL010000705 DAZOYL010000000
VERSION     DAZOYL010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.710"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          121 a           90 c           90 g          123 t
ORIGIN      
        1 cgtgtatttt tagtcattct gtttacctct ttctcagggc gtttagtctc caggatttcc
       61 ggggcggttc aaaaaactct tcgttgatta ctgtggactt actgttggca ttaccgaccc
      121 tgagaccgga gaaataagaa ccgcttagat catcgtggct gttctcgggg tatcaagtta
      181 cacatgggca gaggctaccc aaggtctact attatcgcct gaaagcgctg atggaacagt
      241 gctatcaggg acatgctgat gaaaatacct aaataatcag ccagcactct atctttccaa
      301 atccacagca tagcaaagag agcaaaagag cctgtaaatt cagaaattaa ggaaacgcct
      361 cttggtgcac ataatctatc ttatgttaaa tgaaatctaa tttcttgatt atatgctgga
      421 tcgt
//
LOCUS       DAZOYL010000706          422 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZOYL010000706 DAZOYL010000000
VERSION     DAZOYL010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.711"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>422
                     /locus_tag="ACHUOY_004478"
     CDS             <1..>422
                     /locus_tag="ACHUOY_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0685012.1"
                     /translation="HNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNN
                     KILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTID
                     GLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIV"
BASE COUNT          154 a           78 c           77 g          113 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 tt
//
LOCUS       DAZOYL010000707          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZOYL010000707 DAZOYL010000000
VERSION     DAZOYL010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.712"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHUOY_004479"
     tRNA            41..116
                     /locus_tag="ACHUOY_004479"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZOYL010000708          419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZOYL010000708 DAZOYL010000000
VERSION     DAZOYL010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.713"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            101..376
                     /locus_tag="ACHUOY_004480"
     CDS             101..376
                     /locus_tag="ACHUOY_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR0685013.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT          103 a           99 c           84 g          133 t
ORIGIN      
        1 aagctatttt tattcatcga gaaaatagcc atactgacgc cttcaatgga tattgatatt
       61 cactacagat attgttcgca gcagacagga tgtatttaat atgccaacag ttctctctcg
      121 catggcgatg caactcaaaa aaacagcctg gataattccc gtcttcatgg tttcgggatg
      181 ctcattatct ccggcaatcc cggtgatcgg tgcttattat cccagctggt ttttctgcgc
      241 cattgccagc cttattttga tgctcatcac gaggcgagtt attcagcggg caaatatcaa
      301 tctggcattt gtcggaatta tttataccgc cctttttgct ctctacgcca tgctgttctg
      361 gctggcattt ttctaatcta tttgagacgc gctcatggaa agtacgccga aaaaagctc
//
LOCUS       DAZOYL010000709          419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZOYL010000709 DAZOYL010000000
VERSION     DAZOYL010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.714"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>419)
                     /locus_tag="ACHUOY_004481"
     CDS             complement(<1..>419)
                     /locus_tag="ACHUOY_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
                     /protein_id="HFR0685014.1"
                     /translation="TNLVASSGAHMVQRLLFFVLTILVVKRISSLPLRLLVAAPFVLL
                     TAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKMLGMYIPYLCAFAFLSLLFLA
                     VIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKD"
BASE COUNT          155 a           88 c           85 g           91 t
ORIGIN      
        1 atctttataa gcaaattgac aagcagaaaa taaactgccc gaaatgacaa tcagcaataa
       61 tattcctgtc actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa
      121 aagaagggat aaaaaagcaa aagcacatag ataagggata tacatcccca gcattttgac
      181 aacttcatcc ggatcactct gcagcacact aatcgcaaat ccatcattga aagttgtacc
      241 aaaggtacac catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg
      301 tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt tttacaacca ggatggtcag
      361 gacgaagaac agcaatcgct gaaccatatg cgcgccggaa gaggcgacta agttcgtaa
//
LOCUS       DAZOYL010000710          416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZOYL010000710 DAZOYL010000000
VERSION     DAZOYL010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.715"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>416)
                     /locus_tag="ACHUOY_004482"
     CDS             complement(<1..>416)
                     /locus_tag="ACHUOY_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0685015.1"
                     /translation="PQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLK
                     HENMASSAYIHKNNKLLYNLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFST
                     IFGDKYILTLLNSMRNMDGNKDSRINKNVIVLAGNH"
BASE COUNT          131 a           82 c           64 g          139 t
ORIGIN      
        1 atgatttcca gccagaacga tgacattttt attaatacgg ctatctttat taccatccat
       61 atttcgcata gaatttagca aagtaaggat atatttatca ccaaaaatag tactaaatct
      121 atcgcctgta tgatccccaa ggaagataca aggggttatc gcatcgtcct ttaaaaaaac
      181 atgttgatgc aactcattca atatcatgtc tctcgtattt aataaattgt agagcaattt
      241 attattttta tgtatgtatg cagatgaagc catgttttcg tgttttaata attcacataa
      301 taaactccaa cccttttccc tgatgcctat atgccctgaa acgagcaatg cataaagtac
      361 agcgcctact cttccatctg tatcgccaaa ataagccgtg ttggcatttt gtgggt
//
LOCUS       DAZOYL010000711          413 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZOYL010000711 DAZOYL010000000
VERSION     DAZOYL010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.716"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(3..>413)
                     /locus_tag="ACHUOY_004483"
     CDS             complement(3..>413)
                     /locus_tag="ACHUOY_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0685016.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT          135 a          116 c           91 g           71 t
ORIGIN      
        1 tcctaacgcc gatcgagaga aattgccccc ggcccggtaa ttgccaatag caagaacgcg
       61 ccagcgatac tgacattctt ccagaaatta atcatatttg gcccaaccgc atcgccggtc
      121 atatcccagt aatggtgacc aatcaccgcc gtacccagcg tgtagaaaat aaacagcacc
      181 gccagcggac gggtgaaaaa gccaagcacg attaatatcg cggcgggcac ttccataact
      241 accgcaataa tcgctgccag catcggcatt ggcgcgccca acgaggccat atattggacc
      301 gtaccgtcaa agcccatcat tttgggaaaa ccaaaaataa tgaaaattaa gaccacggcg
      361 atacgggcaa ttaataacaa aacggggcgg gcagctccaa aatcaaaata acg
//
LOCUS       DAZOYL010000712          411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZOYL010000712 DAZOYL010000000
VERSION     DAZOYL010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.717"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(116..>411)
                     /locus_tag="ACHUOY_004484"
     CDS             complement(116..>411)
                     /locus_tag="ACHUOY_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR0685017.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT          123 a          110 c           98 g           80 t
ORIGIN      
        1 tgcgccagtg gcaatatgtg gcttagctca tgaaaggagc gcaacaagat gatgaatcaa
       61 catataacaa catcttaaaa aaaggcctga cattacgcca ggccttctgc gttaattaat
      121 cacgcttttc cagcagggtc cggtaaatca gaccaccgat aatgccgccg acaattggca
      181 ccacccagaa gaaccacagt tgttctaatg cccagccgcc ctggaagata gcaaccgcgg
      241 tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat actaattaag tgaatcaggg
      301 ttaaggccag accaatagcg atcggcgcaa aacctgccgg cgcgaatttg tcggttgcgc
      361 cgtggatcac caacaggaaa cctgcactca ataccagttc aactaccagc g
//
LOCUS       DAZOYL010000713          408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZOYL010000713 DAZOYL010000000
VERSION     DAZOYL010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.718"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHUOY_004485"
     tRNA            54..130
                     /locus_tag="ACHUOY_004485"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHUOY_004486"
     tRNA            173..248
                     /locus_tag="ACHUOY_004486"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAZOYL010000714          406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZOYL010000714 DAZOYL010000000
VERSION     DAZOYL010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.719"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>406)
                     /locus_tag="ACHUOY_004487"
     CDS             complement(<1..>406)
                     /locus_tag="ACHUOY_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0685018.1"
                     /translation="TNATNITGFLNILHAAKNAQVQSFTYAASSSTYGDHPALPKVEE
                     NIGNPLSPYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRRQDPNGAYAAVIPKWT
                     AAMLKGDDVYINGDGETSRDFCYIDNVIQMNI"
BASE COUNT          133 a           74 c           64 g          135 t
ORIGIN      
        1 aatatattca tttgtataac attatctata taacaaaaat cacgactcgt ttcaccatcg
       61 ccattaatat atacgtcatc acctttaagc attgctgctg tccattttgg aattactgca
      121 gcataagctc cattaggatc ttgacgacga ccaaatacat taaaataacg taatccaata
      181 gttttaaaac catatgttcg agcatatacc tgagcataaa tctcgttaac atatttagta
      241 actgcataag gagaaagtgg attaccaatg ttttcctcta cttttggtag tgcgggatga
      301 tctccataag ttgagcttga tgcagcataa gtaaaacttt gtacttgtgc atttttagcc
      361 gcatgtaaga tattcaaaaa tccagtaata ttagttgcat tggttg
//
LOCUS       DAZOYL010000715          406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZOYL010000715 DAZOYL010000000
VERSION     DAZOYL010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.720"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(4..>406)
                     /locus_tag="ACHUOY_004488"
     CDS             complement(4..>406)
                     /locus_tag="ACHUOY_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0685019.1"
                     /translation="EQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAA
                     YAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQGKR
                     HNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT           85 a          110 c          136 g           75 t
ORIGIN      
        1 atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga acagaacgtc
       61 gcagcgtcgt ctcgccaggg cgataagcgc ctgattgtgt cgttttccct gactcatttt
      121 gcgggtgtag taagccctgg agagcggatc cctgagcgcg gcgaaggccg acaggaacaa
      181 cgcccgtttg agagctttat taccccgtcg cgagggatgc tcaccgcgta ttgacgagcc
      241 ggatcgccga gttaccggcg caaggccagc ataagcagcg agatgtgcgg cagaggcgaa
      301 ggcgcggcag gcgacctcgg tgaggagtct ggctgcggtc ctgacaccga ctccgggcat
      361 actggtcagg accgggtaaa gagggtgagc aagaactcgc tgttct
//
LOCUS       DAZOYL010000716          406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZOYL010000716 DAZOYL010000000
VERSION     DAZOYL010000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.721"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            12..83
                     /locus_tag="ACHUOY_004489"
     CDS             12..83
                     /locus_tag="ACHUOY_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685020.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(60..212)
                     /gene="hokA"
                     /locus_tag="ACHUOY_004490"
     CDS             complement(60..212)
                     /gene="hokA"
                     /locus_tag="ACHUOY_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR0685021.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
BASE COUNT          117 a           91 c           79 g          119 t
ORIGIN      
        1 gtgcaatgag aatgcgcaac gccgtaagaa aggcgggaat aacttcccgc caaagactct
       61 tactctttca aattgcaggc taaaaatgcc gccagctcat aactctcctg tttaatatgc
      121 aattcacaca gtgaatctct tatcatccag gtgaaaaata aaagcgtgaa acaaatcact
      181 attaaagaaa gtaatctata tttctgcggc attcccagcc tcctgtgttg atttccaacg
      241 agtaagaggc taaccttatg gtgttcaggc atagggtagc ctcactttga tttatagtca
      301 ggtggggctt ttctctgtct gcctttcggt gaatacctga gacaaacagt ctcaagcacc
      361 cgtggctatt ctagcttaat aagtttgttt cttctccttg atataa
//
LOCUS       DAZOYL010000717          404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZOYL010000717 DAZOYL010000000
VERSION     DAZOYL010000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.722"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>404)
                     /locus_tag="ACHUOY_004491"
     CDS             complement(<1..>404)
                     /locus_tag="ACHUOY_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NEL-type E3 ubiquitin ligase domain-containing
                     protein"
                     /protein_id="HFR0685022.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGL"
BASE COUNT           85 a          120 c          100 g           99 t
ORIGIN      
        1 agacctgatg ctttcagccg gtcagccacc ctctgagggt actcattctc cagcatctca
       61 tatttctgct cttctgccag cgcccagcgg tcagcttccg tacgcttcag tacagcatgc
      121 catggtcccc agagggagaa ccagtccgta aattcattct cttcacggct tctgaccatg
      181 gcttcggcag tgcggaggtc atttgctgtc actcccgaca cgccatagaa acgcatttcc
      241 ttcacggcag tggagagctg aagtttctct gcgagcatgg tctggaaggc caggtagact
      301 tctatctcat ccacaaaatg gagagttctg actttatccc gggcaatgtc ctccagaatt
      361 tcgaggcgga acatttccct gcccagggag agcagagcgc cggt
//
LOCUS       DAZOYL010000718          404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZOYL010000718 DAZOYL010000000
VERSION     DAZOYL010000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.723"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..246)
                     /locus_tag="ACHUOY_004492"
     CDS             complement(<1..246)
                     /locus_tag="ACHUOY_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0685023.1"
                     /translation="MTTVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRT"
     gene            325..404
                     /locus_tag="ACHUOY_004493"
                     /pseudo
     CDS             325..404
                     /locus_tag="ACHUOY_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          112 a           89 c          101 g          102 t
ORIGIN      
        1 tgtccgtatg acggtattga tgccgatttt cagtgtcctg gcggtatcac ggccccctgc
       61 gccgttatgt gccatttcaa cgatctgctc cttaacgccg ggttttctgg cttcataaga
      121 gtaagtgagc tggaaaacac gtttacatga gcgacaacgg aaacgctcat gccgggaaca
      181 actacggcca tgtcgataaa cttcatcgga atgacagcgc ggacaatgaa cagtaacggt
      241 agtcatggaa aacctcaaat accagtagat taccagaaac ttctatcgat tgaagacatt
      301 accttttgcc gcaataggtg gggtaacatt ggtgaaaacg cattacgccg ggtggcagtt
      361 ggaaaaaaat tatctttttt cggctctgat cgtggtggag aaaa
//
LOCUS       DAZOYL010000719          398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZOYL010000719 DAZOYL010000000
VERSION     DAZOYL010000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.724"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>398)
                     /locus_tag="ACHUOY_004494"
     CDS             complement(<1..>398)
                     /locus_tag="ACHUOY_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0685024.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           84 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatactt
//
LOCUS       DAZOYL010000720          397 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZOYL010000720 DAZOYL010000000
VERSION     DAZOYL010000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.725"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>397
                     /locus_tag="ACHUOY_004495"
     CDS             <1..>397
                     /locus_tag="ACHUOY_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR0685025.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGM"
BASE COUNT          104 a           64 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatg
//
LOCUS       DAZOYL010000721          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZOYL010000721 DAZOYL010000000
VERSION     DAZOYL010000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.726"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(14..202)
                     /gene="bcsR"
                     /locus_tag="ACHUOY_004496"
     CDS             complement(14..202)
                     /gene="bcsR"
                     /locus_tag="ACHUOY_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR0685026.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT          114 a           91 c           76 g          114 t
ORIGIN      
        1 attcaccaat cccctacttt tgttgcgcaa actctgccag caacggccag cgttttaatg
       61 ccgcggccaa ctgttcgcgt tgggaaatat cggcataatc aatatcaggc agtgaaaatg
      121 cctgctttaa cgccacaata tcattctgga agatatagcc tatcgcggga tcaggcagag
      181 tatctggttc attgttattc attttgcttg atccctatga aataaccgta ccgttcatga
      241 aacaattcta accgtgagca tttcgccggt ttattgttta cggcgagacg ggaatttgct
      301 cactttttga ctttccgtta cgcaacagaa aaagttaaaa acgaaaagac taaaatcaca
      361 cgtcttgtga ccttctaaat tgattcacaa gctaa
//
LOCUS       DAZOYL010000722          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZOYL010000722 DAZOYL010000000
VERSION     DAZOYL010000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.727"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..356)
                     /locus_tag="ACHUOY_004497"
     CDS             complement(<1..356)
                     /locus_tag="ACHUOY_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0685027.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTA"
BASE COUNT          103 a           99 c          117 g           76 t
ORIGIN      
        1 tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga
       61 ataacggcac tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
      121 acaatctggt gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc
      181 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
      241 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      301 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      361 gcgtttccgg attcttacac agccactgga tcggt
//
LOCUS       DAZOYL010000723          394 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZOYL010000723 DAZOYL010000000
VERSION     DAZOYL010000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.728"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            63..>394
                     /gene="yhaV"
                     /locus_tag="ACHUOY_004498"
     CDS             63..>394
                     /gene="yhaV"
                     /locus_tag="ACHUOY_004498"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR0685028.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          120 a           72 c          100 g          102 t
ORIGIN      
        1 ggaaagaaac ttgtcactgg catggacgtc aacattgatg atgagattgg cgacgacgaa
       61 taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg
      121 aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt
      181 atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata
      241 tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa
      301 ataaagactg gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata
      361 gtgaaaaaga gaaagtcatc attctgggat ggat
//
LOCUS       DAZOYL010000724          394 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZOYL010000724 DAZOYL010000000
VERSION     DAZOYL010000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.729"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>394
                     /locus_tag="ACHUOY_004499"
     CDS             <1..>394
                     /locus_tag="ACHUOY_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0685029.1"
                     /translation="FATALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIRHNNRNV
                     IFLVPHGWSVDIPAMLLAAQGEKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHSREDGI
                     KPFIQSVRQGYWGYYLPDQDHGPEYSEFA"
BASE COUNT          105 a           79 c          107 g          103 t
ORIGIN      
        1 gtttgccaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
       61 aaagttccag aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa tccggcacaa
      121 taacagaaat gtgatttttc tggttcccca tggctggagc gtggatattc ctgcaatgtt
      181 gctggcagct cagggggaaa aaatggccgc catgtttcat cagcaacgaa atccagtgat
      241 tgattatgtc tggaattctg tacggcgtaa attcgggggg cgcttacatt cccgggagga
      301 tgggataaaa ccatttattc agtcagtacg ccagggatac tgggggtatt accttccaga
      361 tcaggatcat ggtcctgaat acagtgaatt tgct
//
LOCUS       DAZOYL010000725          393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZOYL010000725 DAZOYL010000000
VERSION     DAZOYL010000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.730"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          137 a           58 c           75 g          123 t
ORIGIN      
        1 gctttgtatt ttcatttcgg aatactgtta agtaactttg atgaccctaa caatagatcg
       61 gaattttctt agagtcaatg gtcactgttt ttgcttgcaa agcaaatgtt ttggtggtta
      121 taagtcgtct taatatagtc acgcatgcaa cttgttccat gcggagatta aagattggaa
      181 tataacgcga agatggaatt aaattacatt tgcaataatt aatttattat tactcaataa
      241 ttaccgatat tattattgta aattaatcat tagaatgcaa aaagaaatgt aacggcaaaa
      301 taagaggacg atagctgaga aaaaagcgtg ctttcccaat ctggggcaaa tcacgtctga
      361 atgacacctg aaaacaggtg gtagcctcaa gaa
//
LOCUS       DAZOYL010000726          391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.731, whole genome shotgun sequence.
ACCESSION   DAZOYL010000726 DAZOYL010000000
VERSION     DAZOYL010000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.731"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>391)
                     /locus_tag="ACHUOY_004500"
     CDS             complement(<1..>391)
                     /locus_tag="ACHUOY_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685030.1"
                     /translation="YTNAYRGPMETGKIALCSAFIFLVLFAKNELRTKIKFGKLILFA
                     SLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILILKSDFRHKT
                     TLYTINFMLSLCAVIVTETRAAILVFPF"
BASE COUNT          136 a           63 c           83 g          109 t
ORIGIN      
        1 agaatggaaa cactaatatg gctgcacgcg tttcagttac tatgacagca cataagctaa
       61 gcatgaagtt aattgtatat aatgttgttt tatgtctaaa gtcggatttt aaaatgagaa
      121 ttgatgccag taaagaaggg aaaaggatga tgtagcctgc tgttgtagcg tgggaagctg
      181 ataatgcaac acggtcaacg tttaaataga aatgttgcca catggcatgc gcaaaaaaaa
      241 gtaactgcgt tgccagggat gcgaacagaa ttagtttccc aaattttatt tttgttctca
      301 actcattttt agcaaaaaga accaagaaaa taaaagcact acacaaggca attttcccag
      361 tttccattgg gccacgatat gcattggtat a
//
LOCUS       DAZOYL010000727          388 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZOYL010000727 DAZOYL010000000
VERSION     DAZOYL010000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.732"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>388
                     /locus_tag="ACHUOY_004501"
     CDS             <1..>388
                     /locus_tag="ACHUOY_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0685031.1"
                     /translation="TGNMTLEKNGHVILNNSSSNVGQTYVQKGNWHGKGGILSLGAVL
                     GNDNSKTDRLEIAGHASGITYVAVTNEGGSGDKTLEGVQIISTDSSDKNAFIQKGRIV
                     AGSYDYRLKQGTVSGLNTNKWYLTSQ"
BASE COUNT          115 a           67 c           96 g          110 t
ORIGIN      
        1 tcacaggtaa catgactctg gagaaaaacg gtcatgttat tctcaataat agttcgtcaa
       61 atgtcggtca gacctatgtt cagaaaggta attggcatgg aaagggcgga atattatctt
      121 tgggcgcggt tctcggcaat gacaacagta aaactgaccg gctggaaatt gcaggccatg
      181 cgtctggtat tacctatgtt gcagtgacaa atgagggagg ctctggagat aaaactcttg
      241 aaggtgttca aattatttcg acagattctt ctgataagaa tgcttttatt cagaaaggcc
      301 gtattgttgc tggtagttat gactatcgcc tgaaacaggg cactgtatct ggactgaata
      361 ccaataagtg gtatctaact agtcagat
//
LOCUS       DAZOYL010000728          386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZOYL010000728 DAZOYL010000000
VERSION     DAZOYL010000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.733"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>386
                     /locus_tag="ACHUOY_004502"
     CDS             <1..>386
                     /locus_tag="ACHUOY_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685032.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           81 a           98 c          102 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccg
//
LOCUS       DAZOYL010000729          386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.734, whole genome shotgun sequence.
ACCESSION   DAZOYL010000729 DAZOYL010000000
VERSION     DAZOYL010000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.734"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUOY_004503"
     CDS             complement(<1..215)
                     /locus_tag="ACHUOY_004503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685033.1"
                     /translation="MNHYPSVTSLETPEARCRSGVPPLPACRQRESIYGLIELFIQIV
                     HRLSVRSERRLVKTLLADFQRVHGKTAL"
     gene            137..386
                     /locus_tag="ACHUOY_004504"
                     /pseudo
     CDS             137..386
                     /locus_tag="ACHUOY_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611261.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          108 a           91 c          106 g           81 t
ORIGIN      
        1 agtgctgttt taccgtgaac cctctgaaaa tcagcgagca gtgttttaac cagccgacgt
       61 tcagagcgta ctgacagacg gtggacaatt tgaataaaca gttcaatcag accgtagatg
      121 ctctcccgtt ggcgacatgc tgggagcggt ggaacaccgc ttcggcaacg agcttccggc
      181 gtctccagtg aagtgactga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      241 gttcacccgg atgttgggac tttgttgaac cgaagaacac ggcggtgcgg agtccggaga
      301 gtaacagaat agcaaagagc ttcgtgaaaa caataaagtg tgactacatc agtatcatgc
      361 ccaaacctga cgggttaacg gcagca
//
LOCUS       DAZOYL010000730          386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.735, whole genome shotgun sequence.
ACCESSION   DAZOYL010000730 DAZOYL010000000
VERSION     DAZOYL010000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.735"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..379)
                     /locus_tag="ACHUOY_004505"
     CDS             complement(<1..379)
                     /locus_tag="ACHUOY_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0685034.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT           94 a           96 c           87 g          109 t
ORIGIN      
        1 ctaatcccag caccaccaaa tccaggccgc cttcacgcgc cagtttctga tcatgctcgc
       61 ggtagttatc aatagtgagc ttctggatat tctcttcttt gatccccgca ggggtgaaaa
      121 acagattacg cagattggta atcgttacgc cttctccctc tttgccgcga aagggaattt
      181 catcgaaatt atagaaatag cagttatcgt accagggctt acctttaacc agggtggtga
      241 ggtattcata catgcctttg ggcgtgctac cggcggtaat tgccaggtta acacgacgcg
      301 tcttcgacat ataaccaagc agatggtgtg ccgcgacacg gctcatttcc tggtaatctt
      361 cggtaatgat taatttcatc ttaatg
//
LOCUS       DAZOYL010000731          384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.736, whole genome shotgun sequence.
ACCESSION   DAZOYL010000731 DAZOYL010000000
VERSION     DAZOYL010000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.736"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>384
                     /locus_tag="ACHUOY_004506"
     CDS             <1..>384
                     /locus_tag="ACHUOY_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFR0685035.1"
                     /translation="ELIARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSA
                     EYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLL
                     FAYSNRLSNLPNIQENIIMRRFFYF"
BASE COUNT          131 a           83 c           52 g          118 t
ORIGIN      
        1 agaacttatt gcaagagata atttcttaac acatatatct gcattaccac attatctaat
       61 aactttggat gtgtccgaaa atcaattaga gaatctgccg ttattaccag acaccatcaa
      121 atcactaagc gcagagtata ataggttatc cacactgcct tcattaccct tgaatttaaa
      181 aaaacttgag gttaggaaca acgaactgca aactcttcca tctctgcctt ctaatcttaa
      241 gatacttaag gttgcgcaca accatcttac tgaactgccc cctttaccta ggagactgca
      301 acttcttttt gcatatagca atagattaag caacttgcca aacatccaag aaaatattat
      361 catgagaaga tttttttatt ttga
//
LOCUS       DAZOYL010000732          378 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.737, whole genome shotgun sequence.
ACCESSION   DAZOYL010000732 DAZOYL010000000
VERSION     DAZOYL010000732.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.737"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(76..267)
                     /locus_tag="ACHUOY_004507"
     CDS             complement(76..267)
                     /locus_tag="ACHUOY_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685036.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTAACRLT
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT           86 a          106 c           93 g           93 t
ORIGIN      
        1 cataacgcac ctttgagact gtttaacaag tcttaacgat atccctgaaa cgaccactta
       61 acaagtcttt attactcaca cttttgcaaa tgcattaaat gctgcttgta ttattctgat
      121 gccgcgccag cggcatgtaa ggcggcacgc tgcggttaca tgtgataccg gagtaaaacc
      181 gccgaagccc ggcgttagcc ggtactgatt gacagatttc accttaccca tccccagccc
      241 tgctaagcca tacccgtttt cagccatgac agagcttctg tgcgcggtcg gagtggtccc
      301 gacgagggtt tacccgaagt cggggcgtat ctccgcgtta gcgggccgtc agggccgctt
      361 acgagcgtgt actcgata
//
LOCUS       DAZOYL010000733          376 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.738, whole genome shotgun sequence.
ACCESSION   DAZOYL010000733 DAZOYL010000000
VERSION     DAZOYL010000733.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.738"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            200..307
                     /locus_tag="ACHUOY_004508"
     CDS             200..307
                     /locus_tag="ACHUOY_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0685037.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT           97 a           93 c          112 g           74 t
ORIGIN      
        1 cgtagtatcc acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggcttaatc
       61 cctggtggca ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac
      121 aacagaacgg gggctaatct tgaaccatca acaattcaag aatagccgca aaatgttgtc
      181 attacaacaa ggcggctata tgacgctcgc gaagtttgcc atgattttct ggcacgacct
      241 ggcagcaccg atcctggcgg ggattattac cgcagcgatt gtcggctggt ggcgtaaccg
      301 gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca atatgcgccc gaaaccggcc
      361 gcaggggtaa cagcct
//
LOCUS       DAZOYL010000734          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.739, whole genome shotgun sequence.
ACCESSION   DAZOYL010000734 DAZOYL010000000
VERSION     DAZOYL010000734.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.739"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..296
                     /gene="istB"
                     /locus_tag="ACHUOY_004509"
     CDS             <1..296
                     /gene="istB"
                     /locus_tag="ACHUOY_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR0685038.1"
                     /translation="LKQLQKIQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVV
                     SQFPVDKWHGLMENPTTADAILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
BASE COUNT          123 a           80 c           98 g           73 t
ORIGIN      
        1 atctgaaaca gttgcaaaaa atacaggttc taatcctgga cgatctgggg ctggaacagt
       61 taagtaacgc ccagtgtaat gaccttctgg agatcacaga ggatcgttac ggacaaagca
      121 gcacgatagt ggtaagccag ttcccggtag ataaatggca tggtctgatg gagaacccaa
      181 caacagcaga tgccatcctg gacagactgg tacataactc acacagagtg gtccttcagg
      241 gagagtccct gaggaaaaat cccccgacag tggaaagcag cgaaaaaacg agttaaaaag
      301 cggtagaaaa agttaagttc ccggttgatc gaatggatgc gaatcgctga tcgaacttcg
      361 atgccaatcc gtga
//
LOCUS       DAZOYL010000735          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.740, whole genome shotgun sequence.
ACCESSION   DAZOYL010000735 DAZOYL010000000
VERSION     DAZOYL010000735.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.740"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHUOY_004510"
     CDS             <1..>374
                     /locus_tag="ACHUOY_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR0685039.1"
                     /translation="GKEIFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSR
                     PELLSQALSGKTVDLKIVSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLI
                     RNSDGLLKEVSVNLKVVTFNFG"
BASE COUNT          123 a           52 c           96 g          103 t
ORIGIN      
        1 ggggaaggaa attttttcgg gtatcagaca tggagttata tcagcctatg gattgaaaaa
       61 aaactcatct gaaagagctg ttgctgctcg taataaagct gaggagttag taagcgcagc
      121 attatatagc agaccagagt tattatcaca ggctttgtct ggtaaaacag tagatttaaa
      181 gattgtttcg acttctcttc tgacgccgac cagtttaacc gggggagagg agagtatgtt
      241 aaaagatcag gtgaacgcat taaaagggtt aaactctaaa aggggggagc caacgaaatt
      301 attaattcgg aatagtgatg gtcttctgaa agaagtaagc gttaatctaa aagtggtgac
      361 atttaacttt ggtg
//
LOCUS       DAZOYL010000736          373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.741, whole genome shotgun sequence.
ACCESSION   DAZOYL010000736 DAZOYL010000000
VERSION     DAZOYL010000736.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.741"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>373)
                     /locus_tag="ACHUOY_004511"
     CDS             complement(<1..>373)
                     /locus_tag="ACHUOY_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0685040.1"
                     /translation="LGAGEDGMDAWYITSSNPSHASRTKLRINSDIMISAGHGGAGDN
                     NDGNSCGGNGGDSITGSDLSIINQGMILGGNGGSGADHNGDGGEAVTGDNLFIINGEI
                     ISGGHGGDSYSDSDGGNGGDAV"
BASE COUNT          110 a          114 c           52 g           97 t
ORIGIN      
        1 tgacggcatc acctccattc cccccatcac tatcactata actatcgcca ccatgtccac
       61 ctgaaataat ttctccattt attataaaca gattgtctcc tgtaacagcc tcaccaccat
      121 caccgttatg gtcagcaccg ctaccgccgt taccaccaag aatcatgcct tgattgatta
      181 tagacaagtc agatccggta atagagtcac caccattacc gccacaacta ttaccatcat
      241 tattatcacc agcaccacca tgacctgcgc taatcataat atcagagtta atccgtagct
      301 tagttctaga tgcatgagag gggttggaag aagttatata ccacgcatcc attccatctt
      361 caccagctcc caa
//
LOCUS       DAZOYL010000737          371 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.742, whole genome shotgun sequence.
ACCESSION   DAZOYL010000737 DAZOYL010000000
VERSION     DAZOYL010000737.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.742"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(34..306)
                     /locus_tag="ACHUOY_004512"
     CDS             complement(34..306)
                     /locus_tag="ACHUOY_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685041.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           84 a           89 c          100 g           98 t
ORIGIN      
        1 gtgaacaggc gtttcagcgg tcggggctga aaatcaccac cacttcgggt catccgcctt
       61 catctccgct tctgtttcgt ataaatcaaa acgacggcag gtatggcaga acgtgacgta
      121 ttcgtacggt gaatccgggt acattttgtg gaaggtttcc cagaaacaga ccgtgcagga
      181 gggatgaccg gcgataaagt ctgtaaatac ggactgataa gggtgattat tggctctggc
      241 gacggctttc agaacctctt tcaccattct ggtgtggact ttctggtgct ccaggttgtg
      301 tgacatggga actcattctg gatggttact ctgaaagctc atattctgcc acaccccgat
      361 ttgcagccgc c
//
LOCUS       DAZOYL010000738          368 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.743, whole genome shotgun sequence.
ACCESSION   DAZOYL010000738 DAZOYL010000000
VERSION     DAZOYL010000738.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.743"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>368
                     /locus_tag="ACHUOY_004513"
     CDS             <1..>368
                     /locus_tag="ACHUOY_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0685042.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENM"
BASE COUNT          132 a           49 c           63 g          124 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatga
//
LOCUS       DAZOYL010000739          361 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.745, whole genome shotgun sequence.
ACCESSION   DAZOYL010000739 DAZOYL010000000
VERSION     DAZOYL010000739.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.745"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>361
                     /locus_tag="ACHUOY_004514"
     CDS             <1..>361
                     /locus_tag="ACHUOY_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0685043.1"
                     /translation="QRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFNSE
                     TVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPE
                     SNGIAESFVKTIKRDYIS"
BASE COUNT           84 a           85 c          122 g           70 t
ORIGIN      
        1 tcagcgatgg tgctctgacg ggttcgagtt ccgctgtgat aacggagaaa aactgcgagt
       61 cacgttcgcg ctggactgct gtgaccgtga ggcactgcac tgggcggtca ctacgggcgg
      121 cttcaacagt gaaacagtac aggacgtcat gctgggagcg gtggaacgcc gcttcggcaa
      181 cgagcttccg gcgtctccag tagagtggct gacggataat ggttcatgct accgggctaa
      241 tgaaacacgg cagtttgccc ggatgttggg gcttgaaccg aagagcacgg cggtgcggag
      301 tccggagagt aacggcatag cagagagctt cgtgaaaacg ataaagcgtg actacatcag
      361 t
//
LOCUS       DAZOYL010000740          360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.746, whole genome shotgun sequence.
ACCESSION   DAZOYL010000740 DAZOYL010000000
VERSION     DAZOYL010000740.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.746"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            25..>360
                     /locus_tag="ACHUOY_004515"
     CDS             25..>360
                     /locus_tag="ACHUOY_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0685044.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGL"
BASE COUNT           78 a           89 c          103 g           90 t
ORIGIN      
        1 atgtgaacaa cggaggaaaa catcatgcaa tatgcgaaac ctgtcactct gaatgttgaa
       61 gagtgcgacc gtctctcctt tctgccttac ctgtttggca acgattttct gtatgccgag
      121 gcgtatgtgt acgcgctggc gcaaaaaatg atgccggaat atcagggcgg cttctggcac
      181 ttcatccgcc tgccggacgg tggcggttac atgatgccgg atggcgaccg tttccacatg
      241 gtgaacggtg caaactggtt tgaccgtacc gtgagtgctg atgccgcagg catcattctg
      301 acttctcttg tgattaaccg ccagttgtgg ctgtaccacg acagcgggga tgcaggactg
//
LOCUS       DAZOYL010000741          358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.747, whole genome shotgun sequence.
ACCESSION   DAZOYL010000741 DAZOYL010000000
VERSION     DAZOYL010000741.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.747"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            3..>358
                     /locus_tag="ACHUOY_004516"
     CDS             3..>358
                     /locus_tag="ACHUOY_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685045.1"
                     /translation="MKNEYEKNQTYSITLFENTLVCFMRLYNELRRKVNEEDTPCLEC
                     ESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSGGITYTIQYVQNVDIVRVSLPGR
                     GSESITDFKGYYWYGF"
BASE COUNT          130 a           45 c           70 g          113 t
ORIGIN      
        1 atataaaaaa tgagtacgaa aaaaatcaaa catattcaat cacgcttttt gaaaacacac
       61 tggtgtgttt tatgcggtta tacaatgaac tcagacgtaa agtaaatgaa gaggatactc
      121 catgtctgga atgtgaatca ctagaaaaag aatttgagga aatgcagaat gataatgatc
      181 tatcattatt tatgagaaca ttgcgtacta atgatacaca aatttattca ggggtttcag
      241 gaggtattac atatactata caatatgttc aaaatgttga tattgttaga gtgtccttgc
      301 cgggcagagg ttcagagtct atcacagatt ttaaaggtta ttattggtat ggctttat
//
LOCUS       DAZOYL010000742          355 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.748, whole genome shotgun sequence.
ACCESSION   DAZOYL010000742 DAZOYL010000000
VERSION     DAZOYL010000742.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.748"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..205
                     /locus_tag="ACHUOY_004517"
     CDS             <1..205
                     /locus_tag="ACHUOY_004517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685046.1"
                     /translation="ELHIIGGAGENIILGTMNQVLLPLKQDHPGTRWWCSALEMVLSG
                     GVAIIFYAICLLYSSGEFNKFLI"
BASE COUNT          101 a           58 c           81 g          115 t
ORIGIN      
        1 agagctgcac attattggcg gggccgggga aaacatcatt ttgggaacaa tgaatcaggt
       61 actcttacca ctgaagcaag atcatccggg caccaggtgg tggtgcagtg ctttggaaat
      121 ggtcctctcg gggggagttg ccattatttt ctatgctatt tgcttattat attcatcagg
      181 tgaatttaat aaattcctaa tttgaaatat ccactattaa gccagtgttt aacgacgtta
      241 agatggcaat gtgcagatca tgttatcagg gtgggcggta gcgtatattc cttatacaaa
      301 ccatcattca tttcaatgat tgttattgaa ttatttattg ttgtgaataa aaaga
//
LOCUS       DAZOYL010000743          353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.750, whole genome shotgun sequence.
ACCESSION   DAZOYL010000743 DAZOYL010000000
VERSION     DAZOYL010000743.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.750"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>353
                     /locus_tag="ACHUOY_004518"
     CDS             <1..>353
                     /locus_tag="ACHUOY_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HFR0685047.1"
                     /translation="ILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPAL
                     VELVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVER
                     LLNNGKALSNRQAQN"
BASE COUNT          118 a           48 c           73 g          114 t
ORIGIN      
        1 attttgctgg agaatatatc acttaattat cttattcagt atctgaagga tgctaacctt
       61 tatgatcaca gatatcctat aagaggaaat attagtgata aaacattcta tatatctggt
      121 cctccggcat tggttgaact ggtagctaat acagcaactt tgcttgataa gcaggtttct
      181 tctattggga cagataaagt taattttggg gttataaaat taaaaaatac atttgttagt
      241 gacagaactt ataatatgag aggggaggat attgtcatcc caggggtggc tactgttgta
      301 gaacgtttat taaataatgg taaggctctg agtaacagac aagcccaaaa tga
//
LOCUS       DAZOYL010000744          351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.751, whole genome shotgun sequence.
ACCESSION   DAZOYL010000744 DAZOYL010000000
VERSION     DAZOYL010000744.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.751"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            213..>351
                     /locus_tag="ACHUOY_004519"
     CDS             213..>351
                     /locus_tag="ACHUOY_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFR0685048.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT          103 a           64 c           64 g          120 t
ORIGIN      
        1 aacgagttgc atttagttac atgtttaaca cttgatttaa gatttgtaat ggctagattg
       61 aaatcagatg taatccatta gttttatatt ttacccattt agggctgatt tattactaca
      121 cacagcagtg caacatctgt cagtacttct ggtgcttcta ttttagaggc agctgtcagg
      181 tgtgcgatca ataaaaaaag cggggtttca tcatgtttaa tgaagtccat agtattcatg
      241 gtcatacatt attgttgatc actaaacctt ctttgcaggc gacggctctc ttgcagcacc
      301 ttaaacaatc gctggcaatt acaggaaaat tacataatat tcaacgttct c
//
LOCUS       DAZOYL010000745          351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.752, whole genome shotgun sequence.
ACCESSION   DAZOYL010000745 DAZOYL010000000
VERSION     DAZOYL010000745.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.752"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>351
                     /locus_tag="ACHUOY_004520"
     CDS             <1..>351
                     /locus_tag="ACHUOY_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFR0685049.1"
                     /translation="ENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPPAQ
                     VDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIFGG
                     DMLDVSMPRLDGFE"
BASE COUNT           95 a           70 c           77 g          109 t
ORIGIN      
        1 gtgagaatat tattgataag ctgaagcatg atttcgactt catccttatt gatacaggcc
       61 cgcatctgga cgcatttttg aaaaatgcca ttgctgccgc agatatcatg tttacaccgg
      121 tgccgccagc acaagttgat ttccattcca cgcttaaata tttggctcgt ttacctgaac
      181 ttgtacagat tattgaacag gatggatgtt catgccgttt gcaggcaaat attggcttca
      241 tgtcgaaact ggcgaataag tcagatcata aatactgcca tagcctgaca aaagagattt
      301 tcggtggtga catgcttgat gtttcaatgc cacgtcttga tggttttgag a
//
LOCUS       DAZOYL010000746          349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.753, whole genome shotgun sequence.
ACCESSION   DAZOYL010000746 DAZOYL010000000
VERSION     DAZOYL010000746.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.753"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>349
                     /locus_tag="ACHUOY_004521"
     CDS             <1..>349
                     /locus_tag="ACHUOY_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685050.1"
                     /translation="PHNLYDNQIDKVSPVTKNSQQVKGHYGDKFKEMQMFLNQMSNAL
                     QQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNGNCSERLFNFYECCYKFLPRAQP
                     QDKIESYNSALQAF"
BASE COUNT          130 a           62 c           60 g           97 t
ORIGIN      
        1 ccacataatt tatatgacaa ccaaatagat aaggtttcac ctgtaactaa aaactctcag
       61 caagttaaag gccactatgg agataaattt aaagaaatgc agatgttcct taaccagatg
      121 agcaatgcac ttcaacagac tccatctttg tcagagtcaa aggaacacac catagatatt
      181 caaaaaataa cggatgcctt tgtgaaagaa ttccgggggg tattaattga taaaaatggg
      241 aattgttcag aacgtttatt caatttttat gagtgctgct ataagttttt accaagagcg
      301 cagcctcaag ataaaatcga aagctataat tcagcactac aagcttttt
//
LOCUS       DAZOYL010000747          349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.754, whole genome shotgun sequence.
ACCESSION   DAZOYL010000747 DAZOYL010000000
VERSION     DAZOYL010000747.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.754"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..331)
                     /locus_tag="ACHUOY_004522"
     CDS             complement(<1..331)
                     /locus_tag="ACHUOY_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0685051.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYG"
BASE COUNT          130 a           77 c           77 g           65 t
ORIGIN      
        1 ttccgtaacg attgctgcca gaagtacctt ctgtgcagaa aaaaaccaga agcacgagcc
       61 agccaataaa cggaacaaaa aagagcaagg cccaggcacc tgaacggtcg gtatcgtgta
      121 agcgacggat agcaagcgca aggacgggaa ggaaggttgc cagcaaataa agcatagaca
      181 gatatggaag ctccagacct aaaatcaatt gaatgacatt gataatagcg ccgacgatgg
      241 cgttaatcag agtaaacatc cagtactctt tacgacgtgc gcgaccagag aaaccaacat
      301 aattttttaa tacggataaa taccactgca taaaaacatc cctttaatt
//
LOCUS       DAZOYL010000748          348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.755, whole genome shotgun sequence.
ACCESSION   DAZOYL010000748 DAZOYL010000000
VERSION     DAZOYL010000748.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.755"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          104 a           80 c           66 g           98 t
ORIGIN      
        1 tgctatcgga aagaaaaaag gccatcagca aaactctgat ggcctgaatc acctttacca
       61 atattgtatg aaaaaacacg cacgactaat tgacaataat tttcatttcc attaaaaaag
      121 atcgcgtagc attcttaatt catgaggaac tttacccgcc agcaatctga gtagcgaaag
      181 ctgttcatcc ccaatgtttt tgctggcggg tccttttttc tacggtcctc ctccccggag
      241 gggggcattt cattattttt ctaatcgtta ctggtgagaa ggcggaagaa aaagccagtt
      301 cttcgcccct aaataacagc atgattccag tccatgcaaa atctgatt
//
LOCUS       DAZOYL010000749          346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.756, whole genome shotgun sequence.
ACCESSION   DAZOYL010000749 DAZOYL010000000
VERSION     DAZOYL010000749.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.756"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            190..270
                     /gene="shoB"
                     /locus_tag="ACHUOY_004523"
     CDS             190..270
                     /gene="shoB"
                     /locus_tag="ACHUOY_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR0685052.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT          106 a           71 c           67 g          102 t
ORIGIN      
        1 ccgaactggt aaggaacggg ggttttactt tttaacttta attttgtgca gcatgcaccc
       61 tcaacaacag cgattatgga tcagtgtact tatcttttac aagcaacttg caatctttag
      121 cataaaaact cgagccttta cgaagaaagc aatactgaac ggctgcattc cttgtttaag
      181 gaaaaacgaa tgactgattg ccgatacctg attaaacggg tcatcaaaat catcattgct
      241 gttttacagc tgatccttct gttcttataa cacaaggaaa cgtacttaag gtgcgtccgg
      301 tgaaccaatc ggacgcgcct ttaataacta taaataagtg tcttgg
//
LOCUS       DAZOYL010000750          346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.757, whole genome shotgun sequence.
ACCESSION   DAZOYL010000750 DAZOYL010000000
VERSION     DAZOYL010000750.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.757"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(61..>346)
                     /locus_tag="ACHUOY_004524"
     CDS             complement(61..>346)
                     /locus_tag="ACHUOY_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR0685053.1"
                     /translation="LLTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVIEEA
                     VNALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          109 a           66 c           48 g          123 t
ORIGIN      
        1 gggactatat atttcgtttt cagataagac tccttgagag attatctttt atgtaaaatc
       61 tcaaatatca cctgatgtaa tgtcaatgat aatattaagt gcgctctgaa caacatcttt
      121 cttggtgtga ttaacatctg acaaaaagaa catcagcttc tttattatgt tcttgttgtt
      181 tattatttca cgctcacaga ataaagcgtt gactgcctct tctatgacat ttctctcgta
      241 aaaaaattga gagttagcct catataaaat attggccaat ttttcaccaa aaaccatatt
      301 ataataatca ctgtgcatct tcctccccca gggttcagtc aataat
//
LOCUS       DAZOYL010000751          344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.758, whole genome shotgun sequence.
ACCESSION   DAZOYL010000751 DAZOYL010000000
VERSION     DAZOYL010000751.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.758"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..121
                     /locus_tag="ACHUOY_004525"
                     /pseudo
     CDS             1..121
                     /locus_tag="ACHUOY_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            151..>344
                     /locus_tag="ACHUOY_004526"
     CDS             151..>344
                     /locus_tag="ACHUOY_004526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685054.1"
                     /translation="MYICTLCCFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKEFS
                     LTLEFNDTIIESQKVTGNKA"
BASE COUNT          126 a           45 c           61 g          112 t
ORIGIN      
        1 gctaaagatt tctttttata catagaaaca cagttaaaaa ttgcaaaaga tttttgtaga
       61 cctggagaag aagtatcaag ttctattgca agtaaagttt ttcacgcatt tatcgattta
      121 gttaacaaaa tcaggggtaa gaaagacttt atgtatattt gcacgctttg ctgttttgcc
      181 gaggaggtta aaggtgatta ttctcattac aggacctttt tatttgatat tggtaatcaa
      241 tataaggtta aacttacaca aagcggaaaa aaagagttct ctttaacatt agaatttaac
      301 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaa
//
LOCUS       DAZOYL010000752          342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.759, whole genome shotgun sequence.
ACCESSION   DAZOYL010000752 DAZOYL010000000
VERSION     DAZOYL010000752.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.759"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>342
                     /locus_tag="ACHUOY_004527"
     CDS             <1..>342
                     /locus_tag="ACHUOY_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685055.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEGV"
BASE COUNT           82 a          124 c           89 g           47 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg gt
//
LOCUS       DAZOYL010000753          341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.760, whole genome shotgun sequence.
ACCESSION   DAZOYL010000753 DAZOYL010000000
VERSION     DAZOYL010000753.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.760"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>341
                     /locus_tag="ACHUOY_004528"
     CDS             <1..>341
                     /locus_tag="ACHUOY_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0685056.1"
                     /translation="FYRNEKIECACNFLMDKDAQGYIDLSDFDLINCHFKGDVISKVS
                     FLSSNLQHVTFECKEIENCNFTKATVDNVIFKCRRLHNVIFLKTSGECVDFSQNILDT
                     VDFSQSQLGHS"
BASE COUNT          111 a           46 c           63 g          121 t
ORIGIN      
        1 aattctacag aaatgaaaaa atagaatgcg cttgtaattt tctgatggat aaagatgcgc
       61 aggggtatat cgacctgtct gattttgatt taataaattg tcattttaaa ggtgacgtta
      121 tttctaaggt gtctttttta tcatcaaatc tacaacatgt aactttcgaa tgtaaagaaa
      181 ttgagaattg caattttact aaggcaacag ttgataatgt catatttaaa tgtcgacgtt
      241 tacacaatgt gatttttctc aaaacgagtg gcgaatgtgt tgattttagc caaaatattc
      301 ttgatacagt tgacttctcg cagagtcaac ttggtcatag t
//
LOCUS       DAZOYL010000754          340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.761, whole genome shotgun sequence.
ACCESSION   DAZOYL010000754 DAZOYL010000000
VERSION     DAZOYL010000754.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.761"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>340)
                     /locus_tag="ACHUOY_004529"
     CDS             complement(<1..>340)
                     /locus_tag="ACHUOY_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR0685057.1"
                     /translation="SAPVHVFTLNTGRKAKFTEIKVDHDKVDTQTFVVEEVNGREQTA
                     LTPDSLKDITRTIHLQQFYPCIGIRTGDLIEILDGSRRRAAALLCKVGLRVLVTDDEL
                     TVSEAQHLAKD"
BASE COUNT           93 a           82 c           71 g           94 t
ORIGIN      
        1 atctttcgcc agatgctgag cttcagaaac agtaagctca tcatccgtta caagtacacg
       61 taatccaacc ttgcatagca aagctgctgc ccgacgacgg gaaccatcca aaatctcaat
      121 aagatcgcca gtccgaatcc caatacatgg atagaattgt tgtaaatgta tagttctggt
      181 gatgtctttt agtgagtccg gcgtcagggc tgtctgttcg cggccattca cctcttcaac
      241 cacaaaagtt tgagtgtcca ctttgtcatg atcaaccttg atctcagtaa attttgcctt
      301 tcggccagta ttgagggtaa atacatgaac tggagccgat
//
LOCUS       DAZOYL010000755          340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.762, whole genome shotgun sequence.
ACCESSION   DAZOYL010000755 DAZOYL010000000
VERSION     DAZOYL010000755.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.762"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          104 a           72 c           60 g          104 t
ORIGIN      
        1 cagttatctt tttattcata tcatctcctt tccattggtt aatttatcgg aaattcatcc
       61 ccactaacta aaacgatcca cacaataaaa ctaaatctaa ttgcaggcaa tcaattccag
      121 tcgatatatc atctgtatgg aaatcatcgc tcatcactca tcgcagcctg aaaacccaga
      181 gcaacggggc aggtctgcat ataagtggtt acacggcaga attccagagg gttctgtcac
      241 taaattcagt cactgacaaa tttgcatgat gttgaataga cgagattttg tgtcgaaata
      301 gttgtttcag taactgtcac tcaatcggtg gtttatgtga
//
LOCUS       DAZOYL010000756          336 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.763, whole genome shotgun sequence.
ACCESSION   DAZOYL010000756 DAZOYL010000000
VERSION     DAZOYL010000756.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.763"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>336
                     /locus_tag="ACHUOY_004530"
     CDS             <1..>336
                     /locus_tag="ACHUOY_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR0685058.1"
                     /translation="SKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVVDH
                     LPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYC
                     FPTIKSNDNC"
BASE COUNT          106 a           62 c           61 g          107 t
ORIGIN      
        1 agcaaactcc agaaaattat attatgttta tttcttctat gctgcatcta taatttatgg
       61 acattgcgtc ctgtacagat tttgtataca tactctgatg ctggcaattc agtctttctg
      121 gtcgttgatc atctgccatg gactgacagc gataaaataa attggtatct gaagcatcag
      181 aatgaaatta aaaatcaaca tcccctacca gaaggtagtt ggcatacctg gtatgtcatt
      241 gatatcggta atggcttcac tgactataaa aaatatatcg aagggccgta tgaagatctc
      301 tactgtttcc cgacaattaa gagcaatgat aattgc
//
LOCUS       DAZOYL010000757          336 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.764, whole genome shotgun sequence.
ACCESSION   DAZOYL010000757 DAZOYL010000000
VERSION     DAZOYL010000757.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.764"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..156)
                     /locus_tag="ACHUOY_004531"
     CDS             complement(<1..156)
                     /locus_tag="ACHUOY_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR0685059.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNC"
     gene            232..>336
                     /locus_tag="ACHUOY_004532"
     CDS             232..>336
                     /locus_tag="ACHUOY_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0685060.1"
                     /translation="MAKIDVVCPRYSETQGGIRNGHSSSGGLLYRCKQC"
BASE COUNT           85 a           56 c           77 g          118 t
ORIGIN      
        1 gcaatttaga tttaataaaa aactcctctt gtcagaaaaa tatggcaatt gttttgagtg
       61 caatagagac tggattgtct caacagagac gttgtcttta tttatcatat tttgcaaaca
      121 tattttcctt gatggaatta aatttactga tggcatatgt atatattcct ttcattgatg
      181 ggttgtgacg cagacttagt gttgttgtac tctttgccct ttggatgatt gatggcaaag
      241 attgatgtgg tttgtccccg ttactctgaa actcaggggg gtatccggaa cggccattcc
      301 agttcagggg ggctgctcta tcgctgtaag cagtgc
//
LOCUS       DAZOYL010000758          335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.765, whole genome shotgun sequence.
ACCESSION   DAZOYL010000758 DAZOYL010000000
VERSION     DAZOYL010000758.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.765"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(48..260)
                     /gene="cspH"
                     /locus_tag="ACHUOY_004533"
     CDS             complement(48..260)
                     /gene="cspH"
                     /locus_tag="ACHUOY_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR0685061.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT          100 a           75 c           72 g           88 t
ORIGIN      
        1 tgcactattc caataaaacc agtcagcttt aaacaagcag cgtcatatta agagagataa
       61 acatttgccg ctgttggtcc tcgcaggcca tttacgcggc aaaattccac acgtaatcct
      121 ggtataagca cttctgcgtc gcggggagtg aatgcggaaa tatggacctg aacttcttta
      181 cgaccgtcgg aggggataat gaatcctttg ccgcttttgc gatcaaaggt tttgacaatt
      241 cctgtcattt tacgggacaa acaaattcct tactgaaaat actgcgctgc actatacggg
      301 gttaataaaa taaagccagc gatatttaag accgc
//
LOCUS       DAZOYL010000759          334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.766, whole genome shotgun sequence.
ACCESSION   DAZOYL010000759 DAZOYL010000000
VERSION     DAZOYL010000759.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.766"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          104 a           83 c           69 g           78 t
ORIGIN      
        1 gatgctataa gccagaggct cttctatgag aatatgggga ccattccctc gcgatattaa
       61 cgtaatatct taattgtatg caccgccaga atgcgttcag tgcgatcacg gcagcagaca
      121 ggtaaaaata gcaacaaacc acccgaaaaa ccgccgcgat cgcgtatgat aaattttaac
      181 cacatgcata cctgtgcagc aaggcgaatc acgcacggat ttccagccag agacacctga
      241 aacgtaaccg gctcatttaa accgtctggt ttgttacctc cggttccaca aaaataatgt
      301 ccatcatttt gaagtggaca ctatcgtatg gaat
//
LOCUS       DAZOYL010000760          330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.767, whole genome shotgun sequence.
ACCESSION   DAZOYL010000760 DAZOYL010000000
VERSION     DAZOYL010000760.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.767"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(1..330)
                     /locus_tag="ACHUOY_004534"
                     /pseudo
     CDS             complement(1..330)
                     /locus_tag="ACHUOY_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          100 a           78 c           55 g           97 t
ORIGIN      
        1 ctaatttata cctactctga ccaagtctgg ataagaaagg cacacctgca catttctact
       61 tggaaatgtt ttcttttacc attttgctca aaaatttcaa catccaatat tcctcgtaca
      121 gaactactca agtcctgaat agaaaaagtt ccagctggta cagtagagtc atatattact
      181 cgaccttgtt gagatacaac aacacgtgca ttagtttctg caataccagt aatctgaggc
      241 gcataaccac gaatatttgg cggtagcatt cgttcatcgc tttctaatgc aattcctgtg
      301 taacgccaag caacagcgca ttctggcgca
//
LOCUS       DAZOYL010000761          328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.768, whole genome shotgun sequence.
ACCESSION   DAZOYL010000761 DAZOYL010000000
VERSION     DAZOYL010000761.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.768"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            89..289
                     /gene="glgS"
                     /locus_tag="ACHUOY_004535"
     CDS             89..289
                     /gene="glgS"
                     /locus_tag="ACHUOY_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR0685062.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT           91 a           58 c           86 g           93 t
ORIGIN      
        1 gtttaaaggc actacactga ttggggaata ctgaaatcag aaaaagcata tggccttaaa
       61 aggtatgcga taaccggagg atgtgcttat ggatcatagt cttaattctt taaataattt
      121 cgatttcctg gcgcgtagtt ttgccagaat gcacgcagaa ggtcgcccgg tcgatattct
      181 tgccgttact ggtaacatgg atgaagaaca tagaacctgg ttttgcgcac gttatgcctg
      241 gtattgccaa cagatgatgc aggcaagaga gctggagtta gagcactgat ataacgggcc
      301 tgatggcccg ttttagtgtt tgtattag
//
LOCUS       DAZOYL010000762          327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.769, whole genome shotgun sequence.
ACCESSION   DAZOYL010000762 DAZOYL010000000
VERSION     DAZOYL010000762.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.769"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>327)
                     /locus_tag="ACHUOY_004536"
     CDS             complement(<1..>327)
                     /locus_tag="ACHUOY_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0685063.1"
                     /translation="NVESFLALTATEQMDYDSPMMVTTQAMQSGQTITDNVQRAPKTI
                     SISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQKQIITIVCRDGIAITDSIITQF
                     KASKEHT"
BASE COUNT           92 a           73 c           62 g          100 t
ORIGIN      
        1 gtatgctctt tactcgcttt aaactgagta atgatactat cagtgatagc tattccatct
       61 ctacaaacaa tagtgatgat ttgcttctgg tctcgccatt gttcaaccgt agaaataaag
      121 gtttctacaa gttgcccctg tctggttaat agaactgaac ccgtcgaatc aaccactaca
      181 acaccgctaa tagagatggt cttaggtgct ctctgaacat tatcagtgat agtttgcccc
      241 gattgcatag cctgagttgt caccatcatc ggtgagtcat agtccatctg ttcagtagct
      301 gttaaggcaa gaaaagattc cacatta
//
LOCUS       DAZOYL010000763          326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.770, whole genome shotgun sequence.
ACCESSION   DAZOYL010000763 DAZOYL010000000
VERSION     DAZOYL010000763.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.770"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           97 a           59 c           51 g          119 t
ORIGIN      
        1 tttaattcag cgagacgatc tgctttggta ttcccattgt gttgtttgat gcccatacca
       61 ctcctatata gtacccagac tatgggtcta tggattttgt cattttgttt aaatcaatga
      121 ataatatcct cttatcatca tgatatttgt atggtgcaat catgatgagc ttctcagaga
      181 ataattctct attaccgggc aaaatcagga acacaggaat taatatatat ttaacatccc
      241 ccttacaaag gtgagtttat atcgagttat tccctaagtt gtcgttactt attccaattc
      301 gttactataa atatggcaaa aatatt
//
LOCUS       DAZOYL010000764          324 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.772, whole genome shotgun sequence.
ACCESSION   DAZOYL010000764 DAZOYL010000000
VERSION     DAZOYL010000764.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.772"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            163..>324
                     /locus_tag="ACHUOY_004537"
     CDS             163..>324
                     /locus_tag="ACHUOY_004537"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0685064.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGI"
BASE COUNT          112 a           71 c           58 g           83 t
ORIGIN      
        1 tcacgctgca atactcctta gaatgtatag cgaaaggaga aaaagatata cctcgatcac
       61 ccccctttct cccaagtgaa aataaaaggt tatcaacttg caacattgaa caacattcgt
      121 tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg caatgatatt
      181 ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca attcctgcat
      241 gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac ccgcgatgaa
      301 aagcttatcg cgtgcggtgg aatt
//
LOCUS       DAZOYL010000765          321 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.773, whole genome shotgun sequence.
ACCESSION   DAZOYL010000765 DAZOYL010000000
VERSION     DAZOYL010000765.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.773"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>321)
                     /locus_tag="ACHUOY_004538"
     CDS             complement(<1..>321)
                     /locus_tag="ACHUOY_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFR0685065.1"
                     /translation="VVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVLMN
                     NNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSATI
                     SAPEH"
BASE COUNT           91 a           60 c           75 g           95 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattc gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc aagcttatct ggaaaggggg gaatgctgtc
      301 taatctgttt ccagaaacta c
//
LOCUS       DAZOYL010000766          320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.774, whole genome shotgun sequence.
ACCESSION   DAZOYL010000766 DAZOYL010000000
VERSION     DAZOYL010000766.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.774"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>320)
                     /locus_tag="ACHUOY_004539"
     CDS             complement(<1..>320)
                     /locus_tag="ACHUOY_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFR0685066.1"
                     /translation="DPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNR
                     LFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEE
                     NSRA"
BASE COUNT           91 a           61 c           84 g           84 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc
//
LOCUS       DAZOYL010000767          320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.775, whole genome shotgun sequence.
ACCESSION   DAZOYL010000767 DAZOYL010000000
VERSION     DAZOYL010000767.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.775"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>320
                     /locus_tag="ACHUOY_004540"
     CDS             <1..>320
                     /locus_tag="ACHUOY_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFR0685067.1"
                     /translation="QGVYPTYMPKAEDEIDLFELLGTLWKKKWVILCVTLLTTGLAAV
                     YAFTAKEQWTAKTYIQAPRIAELGSYLKFHQAYARILNQPLDTNALANGLFSDLILIA
                     ESPD"
BASE COUNT           92 a           62 c           73 g           93 t
ORIGIN      
        1 cgcaaggggt ctatccaact tatatgccaa aagcagagga tgaaatcgat cttttcgagc
       61 ttttaggcac cttgtggaag aaaaaatggg ttattttatg tgtcacgttg ctgactacgg
      121 ggttagcagc agtgtatgcc tttaccgcaa aagagcagtg gacggctaaa acctatattc
      181 aagcaccacg tattgctgaa ttaggcagct atcttaaatt tcaccaagcg tatgcccgaa
      241 tattaaatca accgttagat acgaatgcgt tggctaatgg attgttttcc gatttgattt
      301 tgattgctga atcgccagac
//
LOCUS       DAZOYL010000768          318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.776, whole genome shotgun sequence.
ACCESSION   DAZOYL010000768 DAZOYL010000000
VERSION     DAZOYL010000768.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.776"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>318
                     /locus_tag="ACHUOY_004541"
     CDS             <1..>318
                     /locus_tag="ACHUOY_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0685068.1"
                     /translation="TQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMF
                     SPSLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDI
                     LKT"
BASE COUNT           94 a           63 c           70 g           91 t
ORIGIN      
        1 tactcaaatg agtagtcgcc gagctagttc acagcttgta tcttcactta atttgggtga
       61 aggtagtatt cacacatggc gccctgaagc tggcagttat attgctaacc tgatagcaat
      121 gaacacgatg tttagtcctt ctctctatga ccgacacggt agcactattg ttgatcctac
      181 tacaggtcag ctcagcgaaa ccaccatgtg gattcgtact gttggtggac ataatgagca
      241 taatttagct gatagacaat taaaaaccac agctaacagg atggtttatc agattggtgg
      301 agatattttg aagacaaa
//
LOCUS       DAZOYL010000769          317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.777, whole genome shotgun sequence.
ACCESSION   DAZOYL010000769 DAZOYL010000000
VERSION     DAZOYL010000769.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.777"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..210
                     /locus_tag="ACHUOY_004542"
     CDS             <1..210
                     /locus_tag="ACHUOY_004542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685069.1"
                     /translation="TKSIINTVDIHSSYPVHQKQKLTDAGLKLMATAKMPDALRLSGL
                     RHLYNILNLRAFVGRIRRSRSIRHE"
BASE COUNT           94 a           76 c           65 g           82 t
ORIGIN      
        1 acaaaatcta taattaatac cgtagatatt cattcatctt accctgtgca ccaaaagcaa
       61 aaactcactg acgcgggttt gaaactgatg gcaaccgcaa aaatgcctga tgcgctacgc
      121 ttatcaggcc tacgccatct ctacaatata ttgaatttgc gtgcttttgt aggcaggata
      181 aggcgttcac gcagcatccg gcatgaataa agcgcactca acaatctgaa acccgccgga
      241 acggtttatt acgtacatca ggtgaaactg accgataagc cgctttcttt tgggtatagt
      301 gtcgtggaca gtcattc
//
LOCUS       DAZOYL010000770          316 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.778, whole genome shotgun sequence.
ACCESSION   DAZOYL010000770 DAZOYL010000000
VERSION     DAZOYL010000770.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.778"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            71..146
                     /locus_tag="ACHUOY_004543"
     tRNA            71..146
                     /locus_tag="ACHUOY_004543"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
BASE COUNT           86 a           72 c           75 g           83 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt gagtctctcg aattttcgca
      301 acacgatgat gaatcg
//
LOCUS       DAZOYL010000771          315 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.779, whole genome shotgun sequence.
ACCESSION   DAZOYL010000771 DAZOYL010000000
VERSION     DAZOYL010000771.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.779"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          101 a           63 c           47 g          104 t
ORIGIN      
        1 gctttaagct tcttttcgtc cataatttac ctgtctccgt tgttggagta aacatatcaa
       61 aaacaggcaa ttacacaatt taaattacag tctcaaaaca aacccaacgg tcaatatagt
      121 ttaccagcaa gcttactagc aaagtgctaa tcatttggta tctttcgtgc tattctcctt
      181 ttacgtaagt acttaagggg aacgtattta ctacttttaa agaaagctcc gttactatct
      241 gaaatgcaat gagataataa acgattactc tcggttagta tctcatgatc atttacagac
      301 tttgcataat cgcga
//
LOCUS       DAZOYL010000772          315 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.780, whole genome shotgun sequence.
ACCESSION   DAZOYL010000772 DAZOYL010000000
VERSION     DAZOYL010000772.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.780"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>315
                     /locus_tag="ACHUOY_004544"
     CDS             <1..>315
                     /locus_tag="ACHUOY_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0685070.1"
                     /translation="DLYGKVTALRYFTDDKRDDGDKTYARLGFKGETQINDQMIGFGH
                     WEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGSLDYGRNYGVGYDIGSWTDMLPEFG
                     GD"
BASE COUNT           84 a           60 c           80 g           91 t
ORIGIN      
        1 tcgaccttta tggcaaggtt accgctctac gttattttac tgatgataag cgtgacgatg
       61 gtgataaaac ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat gatcaaatga
      121 ttggttttgg tcactgggaa tatgatttta aaggctataa cgatgaagcc aacggctcgc
      181 gcggcaacaa gacccgtctg gcctatgctg gtttaaaaat tagtgaattt ggctctctgg
      241 actatggtcg taactacggt gtcggctacg acattggttc atggaccgat atgttgccag
      301 aatttggtgg cgata
//
LOCUS       DAZOYL010000773          310 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.781, whole genome shotgun sequence.
ACCESSION   DAZOYL010000773 DAZOYL010000000
VERSION     DAZOYL010000773.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.781"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>310
                     /locus_tag="ACHUOY_004545"
     CDS             <1..>310
                     /locus_tag="ACHUOY_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0685071.1"
                     /translation="WYLCPQVRVADIVQLNGNIRPRSRQWWQLFRMVSQWHVDVVIVE
                     LRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLN
                     T"
BASE COUNT           69 a           67 c           98 g           76 t
ORIGIN      
        1 tggtatctgt gtccgcaggt acgggtcgcg gatatcgtcc agctgaacgg gaatatccgg
       61 ccacgatcac gccagtggtg gcagttattc aggatggtgt ctcagtggca tgttgatgtg
      121 gttatcgttg agctgcgttc gttcagtatt gttgcagcag tagagctgga tgatgccagc
      181 catttacgac cggaacgcag acgccgggat attcttctgg aagaggttct gaggcaggct
      241 ggtattccgt tgctcagaag ccacgatgcc agaaaactgc tgcagatgac cggagaatgg
      301 ctgaatacaa
//
LOCUS       DAZOYL010000774          309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.782, whole genome shotgun sequence.
ACCESSION   DAZOYL010000774 DAZOYL010000000
VERSION     DAZOYL010000774.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.782"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>309)
                     /locus_tag="ACHUOY_004546"
     CDS             complement(<1..>309)
                     /locus_tag="ACHUOY_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFR0685072.1"
                     /translation="LSESDSSGYTGTFDIDNSHDSSLAMIENLDAISSETVPLILLFA
                     ENKINANDMEGLIERIRSQFFIDYGVRLPTILYRTSNELKVDDIVLLINEVRADSFNI
                     "
BASE COUNT          112 a           62 c           38 g           97 t
ORIGIN      
        1 tatatattaa aactatcggc acgcacctca tttattaaca aaacaatatc atcgacctta
       61 agctcattgc ttgttctata taaaatagtt ggaagcctga caccataatc aataaagaac
      121 tgacttctta tcctttcaat aagaccttcc atatcatttg catttatctt attttcggca
      181 aataataaaa ttaaaggaac ggtttctgaa ctaatagcat ccagattttc tatcattgcc
      241 aaagatgagt catggctatt atcaatatca aatgtaccag tatagccact agaatcagac
      301 tcagacaga
//
LOCUS       DAZOYL010000775          308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.783, whole genome shotgun sequence.
ACCESSION   DAZOYL010000775 DAZOYL010000000
VERSION     DAZOYL010000775.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.783"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           96 a           58 c           66 g           88 t
ORIGIN      
        1 ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga acgttctgcc
       61 ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa accgtagcac
      121 gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg agtttgaatg
      181 aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac aggaattgct
      241 tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa ctcgaaaaaa
      301 atggtaac
//
LOCUS       DAZOYL010000776          308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.784, whole genome shotgun sequence.
ACCESSION   DAZOYL010000776 DAZOYL010000000
VERSION     DAZOYL010000776.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.784"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>308
                     /locus_tag="ACHUOY_004547"
     CDS             <1..>308
                     /locus_tag="ACHUOY_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR0685073.1"
                     /translation="AVTARQFQRELDLADKAILWALISASTKEGRKACSLSYFACKAA
                     EAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPL
                     "
BASE COUNT          109 a           57 c           63 g           79 t
ORIGIN      
        1 gcagttactg caagacaatt tcagagagag ttagatttgg cagacaaagc aatattatgg
       61 gcactaatat cagccagtac taaagaggga cgaaaagcgt gttcactaag ctattttgcc
      121 tgtaaggccg cagaggctga actcggactg gcatatatgg ctgcgaatga taacaaggaa
      181 tttctcacat ccttatctaa tataatgagg tacaaaatag atgctggact ttccgaatcc
      241 tatacatgct atttattaag taagggaaag atcataagac catatctgaa aaatctaaat
      301 ccactcca
//
LOCUS       DAZOYL010000777          306 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.785, whole genome shotgun sequence.
ACCESSION   DAZOYL010000777 DAZOYL010000000
VERSION     DAZOYL010000777.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.785"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            77..>306
                     /locus_tag="ACHUOY_004548"
     CDS             77..>306
                     /locus_tag="ACHUOY_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR0685074.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSP"
BASE COUNT           98 a           68 c           71 g           69 t
ORIGIN      
        1 gctgattatt aaaaatcaac cacacccgcc agtattctgt atatttactg gcggtcatat
       61 cgtgagaggt atggcaatga atcttgtgac actcaaaacg tggggaaaac tcagatatcc
      121 ggataaccca ccatcaatat caacgctgag acgatgggca aggaatggaa acatttatcc
      181 tgcacctgaa ctacacggga ggagttacag ggtggttccg gaggctttct atatcaaccc
      241 aaataaggtt gataccgata taacacacca tcagcctaat gggcgacaag ggagagacag
      301 tccgtt
//
LOCUS       DAZOYL010000778          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.786, whole genome shotgun sequence.
ACCESSION   DAZOYL010000778 DAZOYL010000000
VERSION     DAZOYL010000778.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.786"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..219)
                     /locus_tag="ACHUOY_004549"
     CDS             complement(<1..219)
                     /locus_tag="ACHUOY_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0685075.1"
                     /translation="MTNGISTSPHCLYKSNIVDDVIINKTRQNELVKVFCEYKTEFLI
                     LFDDFFRSHNLPKPSPVLHHFFQYTHLRD"
BASE COUNT          104 a           37 c           45 g          115 t
ORIGIN      
        1 atctctcaga tgagtatatt gaaaaaaatg atgaaggact ggagatggtt taggcaagtt
       61 atggcttcta aaaaagtcat caaacaaaat taagaactct gttttatact cgcaaaatac
      121 ttttactaac tcattttgtc ttgttttatt tattattaca tcatctacta tatttgattt
      181 ataaagacaa tgtggcgaag ttgagatacc atttgtcatt atcagtcctt gaagttgatg
      241 aatttttatt aatttaattt atgagtgaat gtgactacta tcaaaaatag ctcattaatt
      301 t
//
LOCUS       DAZOYL010000779          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.787, whole genome shotgun sequence.
ACCESSION   DAZOYL010000779 DAZOYL010000000
VERSION     DAZOYL010000779.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.787"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHUOY_004550"
     CDS             <1..>301
                     /locus_tag="ACHUOY_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0685076.1"
                     /translation="PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKD
                     CRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRAS"
BASE COUNT           76 a           56 c          100 g           69 t
ORIGIN      
        1 cgccggtatg ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa
       61 aagtcaggga ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca
      121 ccgcctgtaa ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac
      181 gtggtatgcc tgttatcaga gactgccagc gttgtggtgg tcgtggctat gaaagactac
      241 catcaacgga ggcatttaat gctatatgcg aggtgacaaa ccagataaca cgcgcgtcat
      301 g
//
LOCUS       DAZOYL010000780          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.788, whole genome shotgun sequence.
ACCESSION   DAZOYL010000780 DAZOYL010000000
VERSION     DAZOYL010000780.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.788"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHUOY_004551"
     CDS             <1..>301
                     /locus_tag="ACHUOY_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685077.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAZOYL010000781          299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.789, whole genome shotgun sequence.
ACCESSION   DAZOYL010000781 DAZOYL010000000
VERSION     DAZOYL010000781.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.789"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..70)
                     /locus_tag="ACHUOY_004552"
     CDS             complement(<1..70)
                     /locus_tag="ACHUOY_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685078.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLK"
BASE COUNT           96 a           65 c           40 g           98 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagtt
//
LOCUS       DAZOYL010000782          298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.790, whole genome shotgun sequence.
ACCESSION   DAZOYL010000782 DAZOYL010000000
VERSION     DAZOYL010000782.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.790"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..298
                     /locus_tag="ACHUOY_004553"
                     /pseudo
     CDS             1..298
                     /locus_tag="ACHUOY_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           73 a           80 c           89 g           56 t
ORIGIN      
        1 gactggtctg ccaccgcgct ggatggcagt aatatccggg cgctgaaatg tgccgccggt
       61 gctcaaaaaa acatcccgat atcgacggag ataatgggcc gggtcgctct cgcggcggtt
      121 ttggcaccaa aatccatctg gcaacagacg gaagtggcct cccgttaaat atcgtgctaa
      181 gccccggaca agctcatgaa agccagttcg cgcaacgact tctggacggg attggcgttc
      241 agcgtcagaa cggcagcatg aaacgccgtg gacatgcggt actggctgat aaagcgta
//
LOCUS       DAZOYL010000783          298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.791, whole genome shotgun sequence.
ACCESSION   DAZOYL010000783 DAZOYL010000000
VERSION     DAZOYL010000783.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.791"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..138
                     /locus_tag="ACHUOY_004554"
     CDS             <1..138
                     /locus_tag="ACHUOY_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685079.1"
                     /translation="LLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYT
                     L"
BASE COUNT           91 a           62 c           59 g           86 t
ORIGIN      
        1 ttattgctct ctgttctgga cgtaaaaaca tacagagatg atgcacgatt aaatgaatgg
       61 atatctaata gagtgcagga attaggtgac agatattatc ctgtaaataa gcacgttaaa
      121 atccgctaca ctctttagtt cagaaagggt tccacctgca agttgttatt ccaccagaga
      181 gtgaatcgca aagcaggtaa tcatttcatc tgtatatccg tatgccaaat ggcattccac
      241 cgccttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagc
//
LOCUS       DAZOYL010000784          297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.792, whole genome shotgun sequence.
ACCESSION   DAZOYL010000784 DAZOYL010000000
VERSION     DAZOYL010000784.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.792"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            69..>297
                     /locus_tag="ACHUOY_004555"
     CDS             69..>297
                     /locus_tag="ACHUOY_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR0685080.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKS"
BASE COUNT           77 a           49 c           78 g           93 t
ORIGIN      
        1 gcggttaatt cgttgtgtat ttaattatac aatgattccg gtgttctgga atttatttag
       61 aggaatctat gcgagggaaa aaacgcattg ggttattgtt tttgctgata gcggttgtgg
      121 ttggtggcgg cgggttattg ctggcgcaaa aagccttaca taaaacgtcg gatacagcat
      181 tttgcctttc ctgccactcg atgagtaaat cttttgagga atatcaggga actgtccact
      241 tttcgaacca gaaagggata cgtgcggaat gtgcagattg ccatattcca aagtcag
//
LOCUS       DAZOYL010000785          296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.793, whole genome shotgun sequence.
ACCESSION   DAZOYL010000785 DAZOYL010000000
VERSION     DAZOYL010000785.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.793"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           84 a           68 c           43 g          101 t
ORIGIN      
        1 catggtttat tcctcttcat ctgaattgat ttacattagg ttggttattt tctcataccc
       61 acaactatat tttctctctg aatttattac gtgcaagaca atcgcagcac tctaactaac
      121 ttcgatgcaa tttgatgttc agaaaaatcc ctcattagat tattatcatg aaatacagct
      181 aatcatcttt agcttatata tttctcaatg tattcttttg aaaggatccg tgccctttca
      241 aactcttcag cagggcaaga ttacgaaagc ccgctccccg caaggactga cgccag
//
LOCUS       DAZOYL010000786          296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.794, whole genome shotgun sequence.
ACCESSION   DAZOYL010000786 DAZOYL010000000
VERSION     DAZOYL010000786.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.794"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            35..>296
                     /locus_tag="ACHUOY_004556"
     CDS             35..>296
                     /locus_tag="ACHUOY_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0685081.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT           68 a           59 c           86 g           83 t
ORIGIN      
        1 tctggaaata tagatttatc caatgaggtt acgtatgagt agttggcttg cgatgctgca
       61 agatgccgca gagatgtttg tgtttctcgc cgtcgagctt tcgttgctgt ttatagtgat
      121 tagtgccggt gtcagcctga taagacaaaa ggtgccagac cataaaatcc agcagatgat
      181 gggggccaga aaagggagag gctatctcct ggctgctctg ttgggagccg ttaccccgtt
      241 ctgtagttgc tcgacaatac ccatgttacg tggattgttg tcagcgaaag ccgggt
//
LOCUS       DAZOYL010000787          295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.795, whole genome shotgun sequence.
ACCESSION   DAZOYL010000787 DAZOYL010000000
VERSION     DAZOYL010000787.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.795"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>295
                     /locus_tag="ACHUOY_004557"
     CDS             <1..>295
                     /locus_tag="ACHUOY_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFR0685082.1"
                     /translation="RIHKFRTMFIDSEKKGRITVGQDARVTRVGWYLRKYKIDELPQL
                     IDVLSGTMSLVGPRPEVREFIDEYPDDIREKVLSVRPGITDLASIEMVDENEIL"
BASE COUNT           99 a           35 c           77 g           84 t
ORIGIN      
        1 aggatacata aatttagaac gatgtttatc gattcagaaa aaaaaggacg gataacagtt
       61 ggtcaagatg ctcgggtaac cagagttgga tggtatttac ggaagtacaa aatcgatgag
      121 cttcctcaat tgatagatgt tctttctgga acaatgagtt tggttggccc aagaccggaa
      181 gtgagggagt ttattgatga gtatcctgat gatataaggg aaaaagtttt atcggttagg
      241 ccagggataa ctgacttagc atctatagaa atggtagatg aaaatgagat tttgt
//
LOCUS       DAZOYL010000788          292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.796, whole genome shotgun sequence.
ACCESSION   DAZOYL010000788 DAZOYL010000000
VERSION     DAZOYL010000788.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.796"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>292
                     /locus_tag="ACHUOY_004558"
     CDS             <1..>292
                     /locus_tag="ACHUOY_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0685083.1"
                     /translation="RGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNG
                     GDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEW"
BASE COUNT           78 a           56 c           80 g           78 t
ORIGIN      
        1 atcgtgggcc gcaaagttta cgttgtctgc agttactgga acaacattgg gtttgtgcgg
       61 taagaggcaa tcatgaacag atggcgatgg atgcgctggc atcccagcag atgtctttgt
      121 ggttgatgaa tggcggcgac tggtttattg cgctggcaga taatcaacag aaacaagcga
      181 aaacggcgct ggaaaaatgt cagcatttgc cctttattct tgaagtacac agtcgtaccg
      241 gcaaacatgt tattgctcat gccgattatc cagatgatgt ttatgaatgg ca
//
LOCUS       DAZOYL010000789          290 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.797, whole genome shotgun sequence.
ACCESSION   DAZOYL010000789 DAZOYL010000000
VERSION     DAZOYL010000789.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.797"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           72 a           75 c           52 g           91 t
ORIGIN      
        1 cccaggtgat taccctgggg ttattttctg aaagtttcct ttgcaaaagg atagcgttgt
       61 aaattacttt aacgaattac tccatgaact cattaaagtt ctttcgtaat actttatcgt
      121 caaacacgtt atagctggca tcaaaaacca cagatttacc ctgccagcgg gtaatcccct
      181 cctctggcgt tttcggttga actggtatcc ccgtggattt gcccctatat ttccagacat
      241 ctgttatcac ttaacccatt acaagcccgc tgccgcagat attcccgtgg
//
LOCUS       DAZOYL010000790          289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.798, whole genome shotgun sequence.
ACCESSION   DAZOYL010000790 DAZOYL010000000
VERSION     DAZOYL010000790.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.798"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..241
                     /locus_tag="ACHUOY_004559"
     CDS             <1..241
                     /locus_tag="ACHUOY_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0685084.1"
                     /translation="NISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMIARQ
                     MNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT           95 a           58 c           59 g           77 t
ORIGIN      
        1 gaacatcagt caggctatga ttataccgct tttcccggtt tatgatgaaa aaaaacattt
       61 cctgactatt gaagttcggc caccaatgga tgcatgcatt gccagcgcgg acaataaaat
      121 gattgcccga caaatgaaca aaacagtgga aattttggtg gggtcacatc cggaacagta
      181 tatctgggtt ttaaaattgt taaaaacgcg caaatcaaac gaagcggacc cgtacccttg
      241 aaattataaa gtacaggtat ttccactagt tgtttcttac aggttacca
//
LOCUS       DAZOYL010000791          289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.799, whole genome shotgun sequence.
ACCESSION   DAZOYL010000791 DAZOYL010000000
VERSION     DAZOYL010000791.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.799"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..202)
                     /locus_tag="ACHUOY_004560"
     CDS             complement(<1..202)
                     /locus_tag="ACHUOY_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR0685085.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCN"
BASE COUNT           97 a           58 c           49 g           85 t
ORIGIN      
        1 ggttacagaa gaccttttca ctaacccatg attgacggct tatatcattg gtgaatcgtg
       61 gtaacagcgt gtcatgctca gttgcagcac catctagcgt aaaaccaaat tcacggtgta
      121 agttcttaaa taacaacaat ggagttctcc agtaatcaga acccccatga tgtttcccga
      181 ttaaaatatg ctcaattgac ataagaaata aaaaatttta ttgcaatcac tcttgacaag
      241 gtttactctc ttttaagata agagatactt aacaggcttt ctaaacgga
//
LOCUS       DAZOYL010000792          289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.800, whole genome shotgun sequence.
ACCESSION   DAZOYL010000792 DAZOYL010000000
VERSION     DAZOYL010000792.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.800"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..287
                     /locus_tag="ACHUOY_004561"
     CDS             <1..287
                     /locus_tag="ACHUOY_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HFR0685086.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT           92 a           58 c           73 g           66 t
ORIGIN      
        1 ttgatgttga tgcggcaatg aagaaagtct ctgaactgga aacgctggta gcgcaggcca
       61 aagaagcgga taaaggcggt atgaatttct cgttcattaa ttcggcagac cagtatcaac
      121 tcgagactaa aaaatatgtt cgccgcgtca gagataaagt cccgtactct gactgggata
      181 aagagcatct tcaggatgca aacacaagct ggatggtcga agactcattt ccacgagcgt
      241 tacgcgagta caacgaaatg gttgatgact ataatagcct gcgttaaac
//
LOCUS       DAZOYL010000793          288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.801, whole genome shotgun sequence.
ACCESSION   DAZOYL010000793 DAZOYL010000000
VERSION     DAZOYL010000793.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.801"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           80 a           65 c           63 g           80 t
ORIGIN      
        1 gcccgaggta tattctgaaa tatcattcat catgaccggc gtttatacaa acgccggtca
       61 ttcgctaaca gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg
      121 ttcataagcg tattatcagg attgtctgga aaattcccga tactttggac tattactcct
      181 gaacaattcg aaattcagca gattaaaggt aagcgttaac ggaggtaagc gttaacggag
      241 gtaagcgtaa accgactgcc gtatgtagcc attaagccag tattggta
//
LOCUS       DAZOYL010000794          288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.802, whole genome shotgun sequence.
ACCESSION   DAZOYL010000794 DAZOYL010000000
VERSION     DAZOYL010000794.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.802"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           98 a           47 c           63 g           80 t
ORIGIN      
        1 tatgtcgata tattacccaa tggaaatagt tacttttttc taccctaaca tactgagaat
       61 ctgaggcaac ccctgatggc aaatgtggca tgaatattga tgcgagcaaa tcgccggaat
      121 ttcccgtgat ataagggctg agagcaaatc gagaaaagtt gatatttttt gtcaaagata
      181 gcgaaaaaac gcctgaatat tgtggtttca ggcgaataat gcaacagagt atcagtgtag
      241 atcaccacaa aatatttgcg tgataaaaag ctatttggcg ggaattat
//
LOCUS       DAZOYL010000795          287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.803, whole genome shotgun sequence.
ACCESSION   DAZOYL010000795 DAZOYL010000000
VERSION     DAZOYL010000795.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.803"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>287
                     /locus_tag="ACHUOY_004562"
     CDS             <1..>287
                     /locus_tag="ACHUOY_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0685087.1"
                     /translation="GIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKV
                     MSMWRYRITRLLSRKYPDLVMPDALAAEGSSKREWNRFLDTHYRRGWNVNV"
BASE COUNT           69 a           72 c           86 g           60 t
ORIGIN      
        1 cggggatatt cacggtgatc cacacatggg ggcgtgacca gcagtggcat ccgcatatcc
       61 atttatcgac aactgccggt ggtgtgacgt cgggccacac ctggaaaaat cttcattttt
      121 acgcccgtaa ggtgatgagc atgtggcgct accggataac acggttgctg tcacggaaat
      181 accctgacct ggtgatgccg gatgcgctgg cagcagaagg aagcagtaaa cgggaatgga
      241 atcgcttcct ggacacgcat taccgccgtg gctggaatgt caacgta
//
LOCUS       DAZOYL010000796          286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.804, whole genome shotgun sequence.
ACCESSION   DAZOYL010000796 DAZOYL010000000
VERSION     DAZOYL010000796.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.804"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           97 a           67 c           58 g           64 t
ORIGIN      
        1 ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt tcccggggtt tgcttagaat
       61 attttgtaag ttgtccgaag agatatttac aacaccagaa tgatgaatca tcagtcccct
      121 gccagaggtt cactacgtaa cctgaccgac aatgcaccat tctggtgctg aaaaaagagc
      181 actggaactg caacaaaaca acatcacaga acaggagatg gtaaggagtc agacattccc
      241 acacaatatt gtgtcagtgc aaataacaac cttcgtctca aatctc
//
LOCUS       DAZOYL010000797          285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.805, whole genome shotgun sequence.
ACCESSION   DAZOYL010000797 DAZOYL010000000
VERSION     DAZOYL010000797.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.805"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           70 a           69 c           66 g           80 t
ORIGIN      
        1 aacgatttga gatatgacct aaaagctcgg ccaaataata atgtcgtcga ttatcgaagt
       61 catcatgagt gattctgtcc ccacagtgca gcatcagcat gacagtgaat tgtctggcgt
      121 ggtccattta atgagatgga gatggtccct ctttcctaaa tagctgtgcc taataccgct
      181 acacttttgc cagcccatgt ttgcctccgg ggaatgggct gacggttcct tcaaaatcca
      241 ttgcacgcag ttatgtcgga gcgggaactt gactcccgtc tttac
//
LOCUS       DAZOYL010000798          284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.806, whole genome shotgun sequence.
ACCESSION   DAZOYL010000798 DAZOYL010000000
VERSION     DAZOYL010000798.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.806"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..284
                     /locus_tag="ACHUOY_004563"
                     /pseudo
     CDS             1..284
                     /locus_tag="ACHUOY_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170527.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          101 a           56 c           62 g           65 t
ORIGIN      
        1 gtgacttcta tgaagagaaa tacaaatcat attcaccaag tattgaccat ctcgcttata
       61 tggctggtgt atcaaagaga acagcaatca gagccattga gacacttgag aaacacggtt
      121 taattcaggt gcaaaagaat caggggcgtc aaagcactta tcgtgtattc acactggatg
      181 ataagccgga attacttgta agacctacat cacaggaagt tgcagcctca gtaagacaaa
      241 atgacaccac taccagtgac aaaatgtcat gggggtagtg acaa
//
LOCUS       DAZOYL010000799          283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.807, whole genome shotgun sequence.
ACCESSION   DAZOYL010000799 DAZOYL010000000
VERSION     DAZOYL010000799.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.807"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>283
                     /locus_tag="ACHUOY_004564"
     CDS             <1..>283
                     /locus_tag="ACHUOY_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0685088.1"
                     /translation="RLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQVHH
                     FAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGA"
BASE COUNT           72 a           55 c           87 g           69 t
ORIGIN      
        1 cgacttatca gtggaaaaac acagaacgat caggatatat gcgccctggc aaaagagtat
       61 gagatcgcca tgctgttggt gactaaaggt gcagaagggg tggtggtctg ttatcgagga
      121 caagttcacc attttgctgg aatgtctgtg aattgtgtcg atagcacggg ggcgggagat
      181 gcgttcgttg ccgggttact cacaggtctg tcctctacgg gattatctac agatgagaga
      241 gaaatgcgac gaattatcga tctcgctcaa cgttgcggag cgc
//
LOCUS       DAZOYL010000800          283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.808, whole genome shotgun sequence.
ACCESSION   DAZOYL010000800 DAZOYL010000000
VERSION     DAZOYL010000800.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.808"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>283)
                     /locus_tag="ACHUOY_004565"
     CDS             complement(<1..>283)
                     /locus_tag="ACHUOY_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFR0685089.1"
                     /translation="LPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKV"
BASE COUNT           87 a           42 c           71 g           83 t
ORIGIN      
        1 ctaccttcaa atattttaag cgctgaggaa attctggtag ttcctgtagt ctgttgccgg
       61 aaacaaagag ttcttgtagt ctatgaggca attcaggcaa gacagtaagg cgattacctt
      121 caatacgtaa tattgttagc gttgatggaa gctctggtaa cgtctccaga ctattaaaac
      181 gtgccgagag tgattgtaaa tatggtggta atgatggcag acatgacaaa tttcgattga
      241 atgaaatatt aagcttaatc agttgagatg gaagtgtcgg gag
//
LOCUS       DAZOYL010000801          282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.809, whole genome shotgun sequence.
ACCESSION   DAZOYL010000801 DAZOYL010000000
VERSION     DAZOYL010000801.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.809"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..233
                     /locus_tag="ACHUOY_004566"
     CDS             <1..233
                     /locus_tag="ACHUOY_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0685090.1"
                     /translation="NGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEY
                     RRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
BASE COUNT           79 a           67 c           74 g           62 t
ORIGIN      
        1 ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca tcggatggac
       61 gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg tgctttgggc
      121 ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga aattacctgg
      181 cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat taagggacac
      241 agcctagtct ccaggattcc cggggcgttc accacatgca at
//
LOCUS       DAZOYL010000802          282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.810, whole genome shotgun sequence.
ACCESSION   DAZOYL010000802 DAZOYL010000000
VERSION     DAZOYL010000802.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.810"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..83
                     /locus_tag="ACHUOY_004567"
     CDS             <1..83
                     /locus_tag="ACHUOY_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0685091.1"
                     /translation="FWHDLAALVIAGILASMIVNWLNKRK"
     gene            complement(88..>282)
                     /locus_tag="ACHUOY_004568"
     CDS             complement(88..>282)
                     /locus_tag="ACHUOY_004568"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685092.1"
                     /translation="FRLAADSTTVFLFRNPSRLHSVLRIPQNIIRVQSLHFSYEKGRR
                     VSPLRPGPGANCHYGSLTPA"
BASE COUNT           73 a           65 c           85 g           59 t
ORIGIN      
        1 ccttctggca tgatttagcg gctctggtca ttgctggcat tcttgccagt atgatcgtga
       61 actggctgaa caagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
      121 tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa
      181 cgattgcacg cggataatat tctgcggaat gcgcagcaca gagtgaagac gtgacggatt
      241 ccggaacaga aaaacagtcg tagaatcagc ggcaagccgg aa
//
LOCUS       DAZOYL010000803          281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.811, whole genome shotgun sequence.
ACCESSION   DAZOYL010000803 DAZOYL010000000
VERSION     DAZOYL010000803.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.811"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..>281
                     /locus_tag="ACHUOY_004569"
     CDS             1..>281
                     /locus_tag="ACHUOY_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR0685093.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT           81 a           64 c           67 g           69 t
ORIGIN      
        1 atgaaaaagg tatttgcaaa atctcttctg gtcgcagcga tgttttctgt tgcaggctcc
       61 gcattggcag tacaaaagga tattactgta acggccaacg ttgatgccgc tctggatatg
      121 acacagaccg ataacaccgc gttgccgaaa gcagtagaaa tgcaatatct gccggggcag
      181 ggtctgcaat cttaccagtt gatgaccaaa atctggtcta acgacgttac caaagatgta
      241 aaaatgcagt tagtttcccc ggcgcaactg gttcaaagcc t
//
LOCUS       DAZOYL010000804          281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.812, whole genome shotgun sequence.
ACCESSION   DAZOYL010000804 DAZOYL010000000
VERSION     DAZOYL010000804.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.812"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>281)
                     /locus_tag="ACHUOY_004570"
     CDS             complement(<1..>281)
                     /locus_tag="ACHUOY_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0685094.1"
                     /translation="NKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMS
                     HQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKIN"
BASE COUNT           66 a           87 c           52 g           76 t
ORIGIN      
        1 ttgttaatct ttgcatattc aaacgggctg atacccttat ctctgttcgc atccagaaaa
       61 tctgcccacc actgtagcat cagtcgacgt tcttccagat gttctgcttt atggatatac
      121 gcagcacgta caccattacg ctcctgatgg ctcatctgac gttccacagc atcgcgggac
      181 cacaaacctg attcaatcaa tgcactgcac gccatcgccc ggaatccatg gccacagaca
      241 tcctgggttg tgtcataccc catcaccctc agggctttat t
//
LOCUS       DAZOYL010000805          281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.813, whole genome shotgun sequence.
ACCESSION   DAZOYL010000805 DAZOYL010000000
VERSION     DAZOYL010000805.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.813"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT          115 a           47 c           36 g           83 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc a
//
LOCUS       DAZOYL010000806          276 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.814, whole genome shotgun sequence.
ACCESSION   DAZOYL010000806 DAZOYL010000000
VERSION     DAZOYL010000806.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.814"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..255)
                     /gene="ydjO"
                     /locus_tag="ACHUOY_004571"
     CDS             complement(<1..255)
                     /gene="ydjO"
                     /locus_tag="ACHUOY_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFR0685095.1"
                     /translation="MGREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTY"
BASE COUNT           88 a           55 c           48 g           85 t
ORIGIN      
        1 atacgtatca aaactaagtg ctggagctcc tggctttgag gcatcatagt taatgttaga
       61 catataggca atcatacgat tgaagttcga ctctgctatt tcatcgcagt cacattgacc
      121 cgttgatata aagaaagtga gtgccgccat ggtagactca agcctttctt taaaagagtc
      181 gaaagccagg cttttaatat ttaaatcacc ataattactc tgtattaagt ttgtagaaaa
      241 catctcccgc cccatattgt taacaaaatt attatc
//
LOCUS       DAZOYL010000807          275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.815, whole genome shotgun sequence.
ACCESSION   DAZOYL010000807 DAZOYL010000000
VERSION     DAZOYL010000807.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.815"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>275
                     /locus_tag="ACHUOY_004572"
     CDS             <1..>275
                     /locus_tag="ACHUOY_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0685096.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDS"
BASE COUNT          105 a           42 c           42 g           86 t
ORIGIN      
        1 gatttattag aaaaaccatt gaacaatgca ttattagcaa taaggaaaga acatctgcta
       61 ttaatgcctt atgtatgtaa tgaatcgatt tcatacttgc ttggtgaaaa agggatactg
      121 aaagaaatag atgaccttaa cgcagtaaat aattatcttc ttaataataa gaaagctact
      181 gacaatgaaa ttaatgacat caaagttaac ctgtctcata ttcttattga ttccttagat
      241 gatgcaaaag ttaaccttac accggtcatc gattc
//
LOCUS       DAZOYL010000808          268 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.817, whole genome shotgun sequence.
ACCESSION   DAZOYL010000808 DAZOYL010000000
VERSION     DAZOYL010000808.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.817"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>268
                     /locus_tag="ACHUOY_004573"
     CDS             <1..>268
                     /locus_tag="ACHUOY_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685097.1"
                     /translation="TEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRL
                     QDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
BASE COUNT           90 a           69 c           52 g           57 t
ORIGIN      
        1 aacagaggcc accctgtacg aaaacgggct gtatacgcgc cacatcagac tcagtcgccc
       61 agtatctgac agcaaatacc ccttgccacc cggatctcag ctcattaaaa atatgacctt
      121 acgactgcgc ctgcaggatc agcaggaaga aacatccgtg acattatttc agggaaaaat
      181 gagtaccgac ggaaataact attacatcat caaaggtaaa gtaagctcag tacttctgtt
      241 attaaaaatg ctgcagatta atcacgcc
//
LOCUS       DAZOYL010000809          268 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.818, whole genome shotgun sequence.
ACCESSION   DAZOYL010000809 DAZOYL010000000
VERSION     DAZOYL010000809.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.818"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>268
                     /locus_tag="ACHUOY_004574"
     CDS             <1..>268
                     /locus_tag="ACHUOY_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR0685098.1"
                     /translation="RQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVS
                     FSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVA"
BASE COUNT           78 a           62 c           75 g           53 t
ORIGIN      
        1 tccggcagtt attgtggcga taccagatca gacagagtat gagccggcgc ggaaactgct
       61 gggataacag cccaatggaa cgcttcttca ggagtctgaa gaacgaatgg atgccggtgg
      121 tgggttacgt aagcttcagc gaggcagctc acgccataac ggactatatc gttggatatt
      181 acagcgcact aagaccgcac gaatataacg gtgggttacc cccaaacgaa tcggaaaatc
      241 gatactggaa aaactctaac tcggtggc
//
LOCUS       DAZOYL010000810          264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.819, whole genome shotgun sequence.
ACCESSION   DAZOYL010000810 DAZOYL010000000
VERSION     DAZOYL010000810.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.819"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>264
                     /locus_tag="ACHUOY_004575"
     CDS             <1..>264
                     /locus_tag="ACHUOY_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0685099.1"
                     /translation="DVFDDRTSECLSEEIKIYQECHEKFVNFLKANFNQEIYPQLYTP
                     EIFYEACRNLQSFYDHQETSQNAKYSAIDKKKSHLNQEIKKLIK"
BASE COUNT          110 a           46 c           40 g           68 t
ORIGIN      
        1 gatgtttttg atgacagaac aagcgaatgt ctttctgaag agattaagat ataccaagaa
       61 tgccatgaaa aattcgttaa ttttttaaag gcaaacttca accaagaaat atacccacag
      121 ctatatactc ctgaaatatt ttatgaggcc tgcagaaatc tccagtcatt ttatgatcat
      181 caggaaacgt cacaaaacgc aaaatacagt gcaatagaca agaaaaaaag tcatcttaac
      241 caggaaatca aaaagcttat taaa
//
LOCUS       DAZOYL010000811          263 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.820, whole genome shotgun sequence.
ACCESSION   DAZOYL010000811 DAZOYL010000000
VERSION     DAZOYL010000811.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.820"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           83 a           47 c           42 g           91 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cat
//
LOCUS       DAZOYL010000812          262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.821, whole genome shotgun sequence.
ACCESSION   DAZOYL010000812 DAZOYL010000000
VERSION     DAZOYL010000812.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.821"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>262
                     /gene="hlyE"
                     /locus_tag="ACHUOY_004576"
     CDS             <1..>262
                     /gene="hlyE"
                     /locus_tag="ACHUOY_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR0685100.1"
                     /translation="LDQVIPWQTFDETIKELSRFKQEYSQAASVLVGDIKTLLMDSQD
                     KYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILI"
BASE COUNT           78 a           53 c           58 g           73 t
ORIGIN      
        1 tctcgatcag gtcatcccct ggcagacctt tgatgaaacc ataaaagagt taagtcgctt
       61 taaacaggag tattcacagg cagcctccgt tttagtcggc gatattaaaa ccttacttat
      121 ggatagccag gataagtatt ttgaagcaac ccaaacagtg tatgaatggt gtggtgttgc
      181 gacgcaattg ctcgctgcgt atattttgct atttgatgag tacaatgaga agaaagcatc
      241 cgcccagaaa gacattctca tt
//
LOCUS       DAZOYL010000813          262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.822, whole genome shotgun sequence.
ACCESSION   DAZOYL010000813 DAZOYL010000000
VERSION     DAZOYL010000813.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.822"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            3..236
                     /locus_tag="ACHUOY_004577"
     CDS             3..236
                     /locus_tag="ACHUOY_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR0685101.1"
                     /translation="MQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTPV
                     MLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
BASE COUNT           64 a           46 c           80 g           72 t
ORIGIN      
        1 tgttgcaacg tacgctgggc agtggctggg gagtgttgct gccgggattg ctgattgcag
       61 ggctgatgta tgcggattta tcgccggatc agtggcggat tgtcattctg atgggattag
      121 tattgacgcc ggtgatgctg tatcacaaac agttacggca ttacgttttg ctaccatcgt
      181 gcctggcgct tattgctgcc atcatgctga tgataatgaa tttgaatcag ggatgaaaaa
      241 tcaaggaaga aacaagaaag ga
//
LOCUS       DAZOYL010000814          261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.823, whole genome shotgun sequence.
ACCESSION   DAZOYL010000814 DAZOYL010000000
VERSION     DAZOYL010000814.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.823"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>261
                     /locus_tag="ACHUOY_004578"
     CDS             <1..>261
                     /locus_tag="ACHUOY_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IpaC/SipC family type III secretion system
                     effector"
                     /protein_id="HFR0685102.1"
                     /translation="KLGSQLSLIAFDATKSAAENIVRQGLAALSSSITGAVTQVGITG
                     IGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQI"
BASE COUNT           88 a           54 c           58 g           61 t
ORIGIN      
        1 ctaaattggg ctctcaattg tcattgattg cgttcgatgc tacaaaatca gctgcagaga
       61 acattgttcg gcaaggcctg gcagccctat catcaagcat tactggagca gtcacacaag
      121 taggtataac gggtatcggt gccaaaaaaa cgcattcagg gattagcgac caaaaaggag
      181 ccttaagaaa gaaccttgcc actgctcaat ctcttgaaaa agagcttgca ggttctaaat
      241 tagggttaaa taaacaaata g
//
LOCUS       DAZOYL010000815          261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.824, whole genome shotgun sequence.
ACCESSION   DAZOYL010000815 DAZOYL010000000
VERSION     DAZOYL010000815.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.824"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..72)
                     /locus_tag="ACHUOY_004579"
     CDS             complement(<1..72)
                     /locus_tag="ACHUOY_004579"
                     /inference="COORDINATES: protein motif:HMM:NF017712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR0685103.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEE"
BASE COUNT           76 a           53 c           43 g           89 t
ORIGIN      
        1 ttcttctgcg cctttatagg aatctccgct ttgaaatgaa acctgatgca atcccaaatt
       61 agttatgtgc atttaattat cctcagtcat aaatggggca ttgattgcag ttttgtacct
      121 cgttttacaa caaaaaagat acttattcaa cagggttctc tctttttcta taacactccg
      181 agcctgatat atcagacact ttaacttgtg ggatttcatg atgaaacgag cactacatat
      241 gctcacgagg tacatattat g
//
LOCUS       DAZOYL010000816          260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.825, whole genome shotgun sequence.
ACCESSION   DAZOYL010000816 DAZOYL010000000
VERSION     DAZOYL010000816.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.825"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>260)
                     /locus_tag="ACHUOY_004580"
     CDS             complement(<1..>260)
                     /locus_tag="ACHUOY_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685104.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKY"
BASE COUNT           86 a           58 c           66 g           50 t
ORIGIN      
        1 atatttactg tatttccacc acgcaaagcc aaggaagata acaataccgc cgacgttata
       61 aaaaatgatg gtcagaatat tcgcgccagt cgggaatgtc gaagcgacaa accctacggc
      121 aaatatcgca atcagcattg aaacaacgat aataccggtg cggcgggagc ccatacggaa
      181 atcgcgtggc aggtgatcta atttggcgcg taaattcagg taagccagca tgatgaataa
      241 cggcggaagc atggacgctg
//
LOCUS       DAZOYL010000817          259 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.826, whole genome shotgun sequence.
ACCESSION   DAZOYL010000817 DAZOYL010000000
VERSION     DAZOYL010000817.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.826"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            2..>259
                     /gene="istB"
                     /locus_tag="ACHUOY_004581"
     CDS             2..>259
                     /gene="istB"
                     /locus_tag="ACHUOY_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR0685105.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQ"
BASE COUNT           74 a           61 c           76 g           48 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacag
//
LOCUS       DAZOYL010000818          257 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.827, whole genome shotgun sequence.
ACCESSION   DAZOYL010000818 DAZOYL010000000
VERSION     DAZOYL010000818.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.827"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            15..>257
                     /locus_tag="ACHUOY_004582"
     CDS             15..>257
                     /locus_tag="ACHUOY_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFR0685106.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIE"
BASE COUNT          102 a           48 c           49 g           58 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaa
//
LOCUS       DAZOYL010000819          256 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.828, whole genome shotgun sequence.
ACCESSION   DAZOYL010000819 DAZOYL010000000
VERSION     DAZOYL010000819.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.828"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..242
                     /locus_tag="ACHUOY_004583"
                     /pseudo
     CDS             1..242
                     /locus_tag="ACHUOY_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           75 a           70 c           48 g           63 t
ORIGIN      
        1 tttgctgagc cagtttagca ttgccttcta catgaccgat gcctgagccg gtataccgaa
       61 ctttattgtc ctcaaccagt catgttataa gcaagaaatg cactcagcgg atagagcgac
      121 ataatctgaa cctgcggaca catcttaagc ggcttacccg caaaaccata tgcttctcaa
      181 agtctgatga tatgcactac aagatcatcg gctggtatct gacaatcaac caccaccact
      241 aaatctgcgt cacgac
//
LOCUS       DAZOYL010000820          254 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.829, whole genome shotgun sequence.
ACCESSION   DAZOYL010000820 DAZOYL010000000
VERSION     DAZOYL010000820.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.829"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>254
                     /locus_tag="ACHUOY_004584"
     CDS             <1..>254
                     /locus_tag="ACHUOY_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0685107.1"
                     /translation="YSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQWVTAARKG
                     LNTPGSRTVAELESEVMQLRKALNEARLERDILKKATAYFA"
BASE COUNT           77 a           57 c           61 g           59 t
ORIGIN      
        1 tactctacgg agtttaaagc cgaagccgtc aaaacggttc ctgaaaatca gctttcgatc
       61 agtgaaggcg cttcccgatt atctgttcct gaaggcactt taggacaatg ggttaccgcc
      121 gcaagaaaag ggctcaatac accaggctcc cgcacagtgg ctgagctgga atctgaagtt
      181 atgcaactgc gtaaggcttt aaatgaagca cgacttgagc gagatatatt aaaaaaagca
      241 acagcgtatt ttgc
//
LOCUS       DAZOYL010000821          252 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.830, whole genome shotgun sequence.
ACCESSION   DAZOYL010000821 DAZOYL010000000
VERSION     DAZOYL010000821.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.830"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>252
                     /locus_tag="ACHUOY_004585"
     CDS             <1..>252
                     /locus_tag="ACHUOY_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HFR0685108.1"
                     /translation="SETVLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKT
                     LSSDKVGKTGFIENVYANQIAFGRLYREKRKEQVEALGYE"
BASE COUNT           74 a           52 c           77 g           49 t
ORIGIN      
        1 tcagaaacgg tgctgaccgg taatctggtg atggcactgt ttaaccacga caccagtcgc
       61 gatcaggaac cacagttaca cacgcatgcg gtggtgacta atgttacgca gcataacggc
      121 gaatggaaga cactgagcag tgacaaagtg gggaaaacgg ggttcattga gaatgtgtac
      181 gctaatcaga ttgcctttgg caggctctac cgggaaaaac ggaaagagca ggttgaggcg
      241 ctgggctatg aa
//
LOCUS       DAZOYL010000822          249 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.831, whole genome shotgun sequence.
ACCESSION   DAZOYL010000822 DAZOYL010000000
VERSION     DAZOYL010000822.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.831"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..168)
                     /locus_tag="ACHUOY_004586"
     CDS             complement(<1..168)
                     /locus_tag="ACHUOY_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR0685109.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT           80 a           60 c           49 g           60 t
ORIGIN      
        1 atcgataacg tctcccactg agataagtaa atctcgccac ggatcaaaac gacaatgcca
       61 taatttgcgg cgcaactgct caagacaacc gtgtatatcg ccagaaagcc agatatgtcg
      121 ccattgatga cccgcaattc tctgataaac gggcgcaggc tgtttcatca atattttcct
      181 cccgcgctaa agatcacata attttaacaa gaatgttaaa aaacgttgga ctcagacagt
      241 agagtgtgt
//
LOCUS       DAZOYL010000823          249 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.832, whole genome shotgun sequence.
ACCESSION   DAZOYL010000823 DAZOYL010000000
VERSION     DAZOYL010000823.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.832"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>249)
                     /locus_tag="ACHUOY_004587"
     CDS             complement(<1..>249)
                     /locus_tag="ACHUOY_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0685110.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARK"
BASE COUNT           58 a           70 c           55 g           66 t
ORIGIN      
        1 ctttctggca tagctgtttc cggctttggt aatgctgccc tgatgtctgc ttccacccga
       61 tgaatattct gatggcgtga taccaaacca tgacatcagt tgtcgtggat gttcaaaacg
      121 ggtcagatca cccagttcag aaattaatcc aaccgcagta ataaactgaa tccctcgcat
      181 ggcttgcagc gcctctacta ccggatacag cctccattcg gtaacggcct ccttcagggc
      241 ggattccag
//
LOCUS       DAZOYL010000824          248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.833, whole genome shotgun sequence.
ACCESSION   DAZOYL010000824 DAZOYL010000000
VERSION     DAZOYL010000824.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.833"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           81 a           56 c           54 g           57 t
ORIGIN      
        1 ccttgagttt cagctcccaa agcgctgcgc agaaacagga tatagccaac tgcgatacaa
       61 caagtgatat ctgggattag aggacataat cgagaacact ccgtgtgaag agacatatac
      121 agtcccacac ttccctgctg caaaccggcg gatagcccgc agaatttgga gtgcagatta
      181 taaagcaatg cctgtataag accaaatctg ccttcaaaat gataacagta tgtttgtaaa
      241 ttctgtga
//
LOCUS       DAZOYL010000825          245 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.834, whole genome shotgun sequence.
ACCESSION   DAZOYL010000825 DAZOYL010000000
VERSION     DAZOYL010000825.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.834"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           73 a           48 c           40 g           84 t
ORIGIN      
        1 ttttccataa gcataaatct gccactctat ccatatgcag cttagtgttc ttagtgttaa
       61 ttgtaggtgc taacgtaggg tttatcagtt acagcgacct aacacaaaaa gcgacgcaaa
      121 aattagatag tttgtctaaa taattatgtt gccatgcgaa gtatgcatgg ctgcatgtct
      181 gccttccatt taaaatgggc taagacctat aaccctaaat attattcttt attatcttct
      241 ttacc
//
LOCUS       DAZOYL010000826          243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.835, whole genome shotgun sequence.
ACCESSION   DAZOYL010000826 DAZOYL010000000
VERSION     DAZOYL010000826.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.835"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>243)
                     /locus_tag="ACHUOY_004588"
     CDS             complement(<1..>243)
                     /locus_tag="ACHUOY_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="invasion protein InvC; necessary for efficient
                     entry of S.typhimurium into cultured epithelial cells;
                     probable catalytic subunit of a protein translocase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HFR0685111.1"
                     /translation="QFLHTQVGRGLLGAVVNPLGEVTDKFAVTDNSEILYRPVDNAPP
                     LYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS"
BASE COUNT           78 a           63 c           41 g           61 t
ORIGIN      
        1 ctgacgcaaa aatccccatt cgctgtcctt caccacacgt gagtaaagaa tcaataacct
       61 taatacctgt taaaaaaggc ttctcaattg cagccctttc actatatagc ggaggagcat
      121 tatctacagg tcgataaaga atttcactgt tatccgtaac agcaaactta tcagtaacct
      181 cccctaaagg attgactact gctcccaata atccacggcc gacttgcgta tgaagaaact
      241 ggg
//
LOCUS       DAZOYL010000827          242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.836, whole genome shotgun sequence.
ACCESSION   DAZOYL010000827 DAZOYL010000000
VERSION     DAZOYL010000827.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.836"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>242
                     /locus_tag="ACHUOY_004589"
     CDS             <1..>242
                     /locus_tag="ACHUOY_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR0685112.1"
                     /translation="GSVLGMGRVNELCGIDSVKTIEIINLPSYVTTLVPLSKEGLNEI
                     YRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIR"
BASE COUNT           88 a           22 c           60 g           72 t
ORIGIN      
        1 ggttcggtat tggggatggg gcgagtgaat gaattgtgtg gaatagatag cgtgaaaacg
       61 atagagataa ttaatcttcc atcgtatgtt accacgttag ttccgcttag caaggaaggt
      121 cttaatgaaa tatacagata taaagtggtg gtgaatgaaa ttagagatct ttatgctggt
      181 aagataattg atttattgca aatgaagtat ttcagaaaag aaaaatataa caatataaga
      241 tg
//
LOCUS       DAZOYL010000828          242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.837, whole genome shotgun sequence.
ACCESSION   DAZOYL010000828 DAZOYL010000000
VERSION     DAZOYL010000828.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.837"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>242
                     /locus_tag="ACHUOY_004590"
     CDS             <1..>242
                     /locus_tag="ACHUOY_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0685113.1"
                     /translation="LFLDELPEFERRTLDALREPIESGQIHLSRTRAKITYPARFQLV
                     AAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLD"
BASE COUNT           59 a           73 c           53 g           57 t
ORIGIN      
        1 tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc ttgcgagagc
       61 cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc tatccagccc
      121 gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag ggaaaccata
      181 accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg ccctttctcg
      241 ac
//
LOCUS       DAZOYL010000829          241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.838, whole genome shotgun sequence.
ACCESSION   DAZOYL010000829 DAZOYL010000000
VERSION     DAZOYL010000829.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.838"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>241
                     /locus_tag="ACHUOY_004591"
     CDS             <1..>241
                     /locus_tag="ACHUOY_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFR0685114.1"
                     /translation="QETKNMLDQFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISV
                     RNLKLIMESLALWAPREKDVITLVEHVRASLSRYI"
BASE COUNT           80 a           36 c           47 g           78 t
ORIGIN      
        1 tacaagaaac aaaaaatatg ttagatcagt ttgaaaatcg gtatcctgat ctattaaagg
       61 aagtcttccg acatgtgact atacagagaa tttctgaggt attacaaaga ttgcttggag
      121 aaaatatttc tgttcgcaat ttaaaactta ttatggagtc tttggcgctt tgggctccca
      181 gagaaaaaga tgtcataaca ttagttgaac atgtccgtgc atcactttct aggtatattt
      241 g
//
LOCUS       DAZOYL010000830          240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.839, whole genome shotgun sequence.
ACCESSION   DAZOYL010000830 DAZOYL010000000
VERSION     DAZOYL010000830.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.839"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           78 a           55 c           56 g           51 t
ORIGIN      
        1 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
       61 cggtcaaaat gcttctgccg tgaaggaggg agaatgccat ctcatcactt atatctggcg
      121 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      181 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
//
LOCUS       DAZOYL010000831          240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.840, whole genome shotgun sequence.
ACCESSION   DAZOYL010000831 DAZOYL010000000
VERSION     DAZOYL010000831.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.840"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>240
                     /locus_tag="ACHUOY_004592"
     rRNA            <1..>240
                     /locus_tag="ACHUOY_004592"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           61 a           55 c           80 g           44 t
ORIGIN      
        1 ggacgaagac tgacgctcag gtgcgaaagc gtggggagca aacaggatta gataccctgg
       61 tagtccacgc cgtaaacgat gtcgacttgg aggttgtgcc cttgaggcgt ggcttccgga
      121 gctaacgcgt taagtcgacc gcctggggag tacggccgca aggttaaaac tcaaatgaat
      181 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgatgcaac gcgaagaacc
//
LOCUS       DAZOYL010000832          239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.841, whole genome shotgun sequence.
ACCESSION   DAZOYL010000832 DAZOYL010000000
VERSION     DAZOYL010000832.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.841"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            69..>239
                     /locus_tag="ACHUOY_004593"
     CDS             69..>239
                     /locus_tag="ACHUOY_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0685115.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWR"
BASE COUNT           74 a           57 c           54 g           54 t
ORIGIN      
        1 ggtcagaatc tgacgaatta gacaaagtgg tgtccaccaa ataagtagtg ggaaccaaag
       61 tgtcagatat gcagaaaaat gtgactcccg gcaggcgaaa aggctgccct aattatcctc
      121 ccgaatttaa acagcagctc gttgctgcct cctgtgaacc cgggatatcc atctcaaaac
      181 ttgctcttga aaatggcatt aacgccaatc tgttgttcaa atggcgacaa caatggcgc
//
LOCUS       DAZOYL010000833          237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.842, whole genome shotgun sequence.
ACCESSION   DAZOYL010000833 DAZOYL010000000
VERSION     DAZOYL010000833.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.842"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>237
                     /gene="phoN2"
                     /locus_tag="ACHUOY_004594"
     CDS             <1..>237
                     /gene="phoN2"
                     /locus_tag="ACHUOY_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR0685116.1"
                     /translation="VFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMD
                     ISRENTPILYQLLTQVLQDSHDYAVRNAKEYYKRV"
BASE COUNT           71 a           48 c           51 g           67 t
ORIGIN      
        1 agtatttcag gctgacaaag ctcattatga attcggccgc tcgctccggg atgctaatcg
       61 tgtacgtctc gctagcgaag atgcatacta cgagaatttt ggtcttgcat tttcagatgc
      121 ttatggcatg gatatttcaa gggaaaatac cccaatctta tatcagttgt taacacaagt
      181 actacaggat agccatgatt acgccgtgcg taacgccaaa gaatattata aaagagt
//
LOCUS       DAZOYL010000834          235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.843, whole genome shotgun sequence.
ACCESSION   DAZOYL010000834 DAZOYL010000000
VERSION     DAZOYL010000834.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.843"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..224
                     /locus_tag="ACHUOY_004595"
     CDS             <1..224
                     /locus_tag="ACHUOY_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFR0685117.1"
                     /translation="ASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERML
                     DALDDQELVKLVTERSNQPLHDVDLDSYL"
BASE COUNT           63 a           50 c           61 g           61 t
ORIGIN      
        1 gtgcaagtgt cagtgaactg aaaaaaaatc ctatggctac agtcagtgcc ggagatggtt
       61 accctgtcgc cattcttaac cggaatcagc cggcattcta ctgtgtgcct gctgaactgt
      121 atgaaaggat gcttgatgct ctggatgacc aggaactggt gaaactggtt actgaacgca
      181 gcaatcagcc actgcatgat gtggacctgg acagttattt atgacttata cggta
//
LOCUS       DAZOYL010000835          233 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.844, whole genome shotgun sequence.
ACCESSION   DAZOYL010000835 DAZOYL010000000
VERSION     DAZOYL010000835.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.844"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            155..>233
                     /locus_tag="ACHUOY_004596"
     CDS             155..>233
                     /locus_tag="ACHUOY_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685118.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHG"
BASE COUNT           86 a           35 c           40 g           72 t
ORIGIN      
        1 atcaatttga ttattattta tcataaagaa acgtcgcgag attattaaag aatatccatt
       61 aatgtgcaat tgaaatgtga taattatcac tgaaccgttg ttgtcatttg ttttacaaaa
      121 gcaagggatt aaatctcctc caatggacaa aaaaatgaat aatgacaata ccgattacgt
      181 gagtaatgaa tcagggacgc tttcgcgatt atttaaacta cctcagcatg gga
//
LOCUS       DAZOYL010000836          233 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.845, whole genome shotgun sequence.
ACCESSION   DAZOYL010000836 DAZOYL010000000
VERSION     DAZOYL010000836.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.845"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>233
                     /locus_tag="ACHUOY_004597"
     CDS             <1..>233
                     /locus_tag="ACHUOY_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0685119.1"
                     /translation="AWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQDAVDLEIATEE
                     ENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
BASE COUNT           65 a           53 c           66 g           49 t
ORIGIN      
        1 gcctggtatg cttcgataca gcaggagccg aatggctgga agaccgtcac cacacagttc
       61 tacatccagg aattcagtga gcatattgcg ccacttcagg atgctgtaga tctggagatc
      121 gcaacggagg aagaaaactc gttgctggaa gcctggaaga agtatcgggt gttgctgaac
      181 cgtgttgata catcaactgc acctgatatt gagtggccgg aagaaccaga cac
//
LOCUS       DAZOYL010000837          232 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.846, whole genome shotgun sequence.
ACCESSION   DAZOYL010000837 DAZOYL010000000
VERSION     DAZOYL010000837.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.846"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>232)
                     /locus_tag="ACHUOY_004598"
     CDS             complement(<1..>232)
                     /locus_tag="ACHUOY_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR0685120.1"
                     /translation="QKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYR
                     QLAELSERLRLGDISIDEVVKNISPSIELITAD"
BASE COUNT           65 a           61 c           46 g           60 t
ORIGIN      
        1 atcagcagta ataagctcaa tactcggcga gatattcttc acaacttcat caatagatat
       61 atcacccagc ctcagtcgtt cacttaattc agccagttgc ctgtaatcgg cgtatgtcag
      121 ctcagactga acaggaaaaa gagacacaaa atctttaggt acggacgctg cttggagggc
      181 acgagtcacc ttagctgccg acaatccctc tctttccgca atttgcttct gg
//
LOCUS       DAZOYL010000838          232 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.847, whole genome shotgun sequence.
ACCESSION   DAZOYL010000838 DAZOYL010000000
VERSION     DAZOYL010000838.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.847"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           75 a           56 c           32 g           69 t
ORIGIN      
        1 ttaacccgcg ttaaatatcc cacgttggcc agcatctgtc tcacggaaag tcatactgcc
       61 ttactatttc ctcatgttat tctccatatc agataaatag catctgtatt tcaggcatcc
      121 tgaatattgc agcacacact gaataacacc ttattttgtg aaattaatca caaaatataa
      181 aatcacgact taatacggaa aaaactctgt gctactcaga actgtccggt ca
//
LOCUS       DAZOYL010000839          231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.848, whole genome shotgun sequence.
ACCESSION   DAZOYL010000839 DAZOYL010000000
VERSION     DAZOYL010000839.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.848"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(83..>231)
                     /gene="ymgC"
                     /locus_tag="ACHUOY_004599"
     CDS             complement(83..>231)
                     /gene="ymgC"
                     /locus_tag="ACHUOY_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFR0685121.1"
                     /translation="IDQALRLRLKDETSVILSDLYLQILQYIEMHKTTLTDIIINDRE
                     SVLS"
BASE COUNT           76 a           39 c           44 g           72 t
ORIGIN      
        1 actagtctaa aaactagtat taagactatc acttatttaa gtgatactgg ttatctggag
       61 attcaggggg ccagtctaag ttctaagaga gcacggattc cctgtcatta ataatgatat
      121 ccgttagtgt agtcttatgc atttcaatgt actgcaatat ttgcagatag agatcagaaa
      181 gaatcacact ggtttcatct tttaaacgca agcgtaaagc ctgatcaata a
//
LOCUS       DAZOYL010000840          231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.849, whole genome shotgun sequence.
ACCESSION   DAZOYL010000840 DAZOYL010000000
VERSION     DAZOYL010000840.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.849"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            1..231
                     /locus_tag="ACHUOY_004600"
                     /pseudo
     CDS             1..231
                     /locus_tag="ACHUOY_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    34..150
                     /locus_tag="ACHUOY_004600"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT           54 a           65 c           65 g           47 t
ORIGIN      
        1 gcgccgcagt aacgatatcc ttcgccaggc ttccgcttat tttgcgaagg cggagtttga
       61 ccgcctctgg aaaaaatgat gccactgctg gataagctgc gtgagcagta cggggtcgga
      121 ccgctatgca gcgaactgca tattgccccg tcaacgtatt accactgtca gcaacagcga
      181 catcatccgg ataaacgcag tgcccgtgcg cagcgcgatg actggctgaa g
//
LOCUS       DAZOYL010000841          231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.850, whole genome shotgun sequence.
ACCESSION   DAZOYL010000841 DAZOYL010000000
VERSION     DAZOYL010000841.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.850"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..161)
                     /locus_tag="ACHUOY_004601"
     CDS             complement(<1..161)
                     /locus_tag="ACHUOY_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0685122.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQY"
BASE COUNT           56 a           57 c           51 g           67 t
ORIGIN      
        1 tggtattgct tacgccagag aaataactgg ctggctgcta caccatgttg ccgggcaacg
       61 agggagaccg tcatccccgg ttcaaagctc tgctgaacaa ttgcgatctt ttcctgtgtg
      121 gtacgccgtc tgcgtttctc cggccctaag acatcaatca tctgtactcc aatgactagt
      181 ctaaaaacta gtattaagac tatcacttat ttaagtgata ctggttgtct g
//
LOCUS       DAZOYL010000842          229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.852, whole genome shotgun sequence.
ACCESSION   DAZOYL010000842 DAZOYL010000000
VERSION     DAZOYL010000842.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.852"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>229
                     /gene="ospZ"
                     /locus_tag="ACHUOY_004602"
     CDS             <1..>229
                     /gene="ospZ"
                     /locus_tag="ACHUOY_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HFR0685123.1"
                     /translation="TRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEAST
                     PEGSWLTVISGKRPMGQFSVDSLYHPDLHAL"
BASE COUNT           75 a           40 c           49 g           65 t
ORIGIN      
        1 taaccagaag tatggcacaa ttaaaaatag aggagtttat taatgagaaa tccagacgac
       61 ttaattatat gaagacaatg tattctccat gtccggaaga ttttcagcct atttccagag
      121 acgaagccag tacaccggaa ggaagctggt tgacagttat atctggaaaa cgtcctatgg
      181 ggcaattttc tgtggatagt ttgtaccatc ctgacttaca tgcattatg
//
LOCUS       DAZOYL010000843          228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.853, whole genome shotgun sequence.
ACCESSION   DAZOYL010000843 DAZOYL010000000
VERSION     DAZOYL010000843.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.853"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>228
                     /locus_tag="ACHUOY_004603"
     rRNA            <1..>228
                     /locus_tag="ACHUOY_004603"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           67 a           52 c           71 g           38 t
ORIGIN      
        1 ccggagaatg ttatcacggg agacacacgg cgggtgctaa cgtccgtcgt gaagagggaa
       61 acaacccaga ccgccagcta aggtcccaaa gtcatggtta agtgggaaac gatgtgggaa
      121 ggcccagaca gccaggatgt tggcttagaa gcagccatca tttaaagaaa gcgtaatagc
      181 tcactggtcg agtcggcctg cgcggaagat gtaacggggc taaaccat
//
LOCUS       DAZOYL010000844          227 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.855, whole genome shotgun sequence.
ACCESSION   DAZOYL010000844 DAZOYL010000000
VERSION     DAZOYL010000844.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.855"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>227)
                     /locus_tag="ACHUOY_004604"
     CDS             complement(<1..>227)
                     /locus_tag="ACHUOY_004604"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0685124.1"
                     /translation="LNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHDDVF
                     IADNVFPAAPVYRVASLVVLPSENESFGMVL"
BASE COUNT           79 a           61 c           40 g           47 t
ORIGIN      
        1 cagtaccata ccaaacgatt cgttttctga aggcagaacc accagactgg caacccgata
       61 tacgggggcg gcaggaaaaa cattgtcagc aataaaaaca tcatcatgca tgcccatact
      121 gtcaatctga tactgtaaat gctcccgcag ttcaggcgaa ccagacccca caatcaacca
      181 acagaactgt cgtccattca tttttaaatg gaacagtaaa ttcaaca
//
LOCUS       DAZOYL010000845          227 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.856, whole genome shotgun sequence.
ACCESSION   DAZOYL010000845 DAZOYL010000000
VERSION     DAZOYL010000845.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.856"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           64 a           58 c           29 g           76 t
ORIGIN      
        1 cgctggcctt tttcttttac ctcaccgcgg tggataggca ttcacgccgc atccggcaaa
       61 aagctgcccg cacaataaca tcattcttcc tcaacacgtt ttatcgcaaa ttatcatcac
      121 tactgaaacc gattacacca ataacaccag ccaaatattt gtaatatttt catattatta
      181 ttcagttttc acagttgtta catttctttt cagtaaagtc ttaattg
//
LOCUS       DAZOYL010000846          225 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.858, whole genome shotgun sequence.
ACCESSION   DAZOYL010000846 DAZOYL010000000
VERSION     DAZOYL010000846.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.858"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>225
                     /locus_tag="ACHUOY_004605"
     CDS             <1..>225
                     /locus_tag="ACHUOY_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0685125.1"
                     /translation="CTRDYHRIWDDWAREGTTTENRIQAVRLLKICLDTREPVLNLSL
                     LKLRSLPPLPLHIRELNISNNELISLPENSP"
BASE COUNT           67 a           55 c           42 g           61 t
ORIGIN      
        1 gtgtaccaga gattaccacc gcatctggga tgactgggct agggaaggaa caacaacaga
       61 aaatcgcatc caggcggttc gattattgaa aatatgtctg gatacccggg agcctgttct
      121 caatttaagc ttactgaaac tacgttcttt accaccactc cctttgcata tacgtgaact
      181 taatatttcc aacaatgagt taatctccct acctgaaaat tctcc
//
LOCUS       DAZOYL010000847          224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.859, whole genome shotgun sequence.
ACCESSION   DAZOYL010000847 DAZOYL010000000
VERSION     DAZOYL010000847.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.859"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..222)
                     /locus_tag="ACHUOY_004606"
     CDS             complement(<1..222)
                     /locus_tag="ACHUOY_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685126.1"
                     /translation="MIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGDAERILSSGEY
                     WQRQKTLLTEREVSFMKGLFRIVDMKRWVM"
BASE COUNT           59 a           63 c           41 g           61 t
ORIGIN      
        1 cattacccac cgcttcatat ccacaattct gaacagtcct ttcataaaac tgacttccct
       61 ttctgtcagc agcgtcttct gacgttgcca gtattcaccg ctggacagta ttcgctctgc
      121 atcaccggtc cgtatacctg ccgcatgcaa ttgcatcagc aaaggagaat gctgatttac
      181 acttctgagg ataatccgga taagtagcgc tgcaataatc agaa
//
LOCUS       DAZOYL010000848          223 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.860, whole genome shotgun sequence.
ACCESSION   DAZOYL010000848 DAZOYL010000000
VERSION     DAZOYL010000848.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.860"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>223
                     /gene="ipgB1"
                     /locus_tag="ACHUOY_004607"
     CDS             <1..>223
                     /gene="ipgB1"
                     /locus_tag="ACHUOY_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR0685127.1"
                     /translation="IKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSNMDLFNTQM
                     KDLTNLVSSSVYDKIFNESTKVLQIEISAE"
BASE COUNT           92 a           41 c           34 g           56 t
ORIGIN      
        1 ataaaacttg atgaaaaatc agcacaaaca tctatcagtc atacagcact aaacgagatt
       61 gcctcatctg gcctcagagc aaagatttta aaacgctata gcagtaacat ggatttgttt
      121 aacacacaaa tgaaggatct tacaaatcta gtatccagtt ccgtctacga taaaatattt
      181 aatgaatcaa caaaagtttt acaaatagaa ataagcgcag agg
//
LOCUS       DAZOYL010000849          222 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.861, whole genome shotgun sequence.
ACCESSION   DAZOYL010000849 DAZOYL010000000
VERSION     DAZOYL010000849.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.861"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(28..>222)
                     /locus_tag="ACHUOY_004608"
     CDS             complement(28..>222)
                     /locus_tag="ACHUOY_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685128.1"
                     /translation="QGDVWRAVYELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYE
                     RSEKSVGYWVGGIRRAAQKA"
BASE COUNT           54 a           80 c           37 g           51 t
ORIGIN      
        1 tcggttacat cctgtcagca cagaacctca cgctttttga gcggcacgac ggatcccccc
       61 cacccagtat ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat
      121 atccactaat tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc
      181 cggtgactgc aactcataaa ccgcgcgcca cacatcaccc tg
//
LOCUS       DAZOYL010000850          221 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.862, whole genome shotgun sequence.
ACCESSION   DAZOYL010000850 DAZOYL010000000
VERSION     DAZOYL010000850.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.862"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..169)
                     /gene="mxiH"
                     /locus_tag="ACHUOY_004609"
     CDS             complement(<1..169)
                     /gene="mxiH"
                     /locus_tag="ACHUOY_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR0685129.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSE"
BASE COUNT           67 a           41 c           30 g           83 t
ORIGIN      
        1 attcagataa tttactttgg tattcagcca gcaactgtgg attcgaagga tttttagcta
       61 atttatctag tgccaatgtt agttcacctt gtaatgtttg agttccatca tcaaaagttt
      121 cagataatga actcagtgtc caatcatcat tcggtactgt aacactcatt ttatcctcac
      181 ttatttttat ctaaaaaaaa ccagtgttta tcattcaaca t
//
LOCUS       DAZOYL010000851          221 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.863, whole genome shotgun sequence.
ACCESSION   DAZOYL010000851 DAZOYL010000000
VERSION     DAZOYL010000851.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.863"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            33..>221
                     /locus_tag="ACHUOY_004610"
     CDS             33..>221
                     /locus_tag="ACHUOY_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685130.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKESFATSFNQKCLD"
BASE COUNT           81 a           48 c           31 g           61 t
ORIGIN      
        1 gataaacttt ttctccattt ctgaggtgcg ccatgcataa tgtaaataat actcaagcgc
       61 caacattctt atataaggca acttcaccat catcaacaga atacagcgag ttaaaaagca
      121 aaatatccga tatccatagt tcgcaaactt ctctaaaaac accagcatca gtgtctgaaa
      181 aagaaagctt tgcaacgtct tttaatcaga aatgtcttga t
//
LOCUS       DAZOYL010000852          219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.865, whole genome shotgun sequence.
ACCESSION   DAZOYL010000852 DAZOYL010000000
VERSION     DAZOYL010000852.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.865"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHUOY_004611"
     CDS             <1..187
                     /locus_tag="ACHUOY_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0685131.1"
                     /translation="RPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIW
                     DMGRIAMSVAQQPQCHK"
BASE COUNT           69 a           47 c           53 g           50 t
ORIGIN      
        1 ccgccccgtc attgacagag catgggatgc tcaactcagg ctttgtaaga ggtatcgaaa
       61 acttcaggcc aaaggaaaga atgtcaatat tacaattgtt gctgttgcac gtgagctggc
      121 gggttttatc tgggatatgg gccgaatagc aatgtctgtc gcacaacaac cacaatgtca
      181 caaataaaaa atgagaataa ccagttccct gaaggcgac
//
LOCUS       DAZOYL010000853          218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.866, whole genome shotgun sequence.
ACCESSION   DAZOYL010000853 DAZOYL010000000
VERSION     DAZOYL010000853.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.866"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..209
                     /locus_tag="ACHUOY_004612"
     CDS             <1..209
                     /locus_tag="ACHUOY_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR0685132.1"
                     /translation="AVSSPQSNGIAERFVKTMKEDCIAFMPKPRTALHNLAVAIEHYN
                     ENHPHSALGYLSPREYRRQRVMST"
BASE COUNT           69 a           49 c           56 g           44 t
ORIGIN      
        1 cggcagtcag tagccctcag agtaatggaa tagcagaacg gttcgtgaaa acgatgaagg
       61 aagactgcat cgcgttcatg ccgaaaccaa gaacggcatt gcataacctt gcagtggcga
      121 tcgagcatta caatgaaaac catccgcata gtgcgttggg ttatctctca ccaagggaat
      181 atcgacgtca gcgggtaatg tcaacttaag atacaacg
//
LOCUS       DAZOYL010000854          216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.867, whole genome shotgun sequence.
ACCESSION   DAZOYL010000854 DAZOYL010000000
VERSION     DAZOYL010000854.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.867"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           79 a           47 c           20 g           70 t
ORIGIN      
        1 ccccacataa aaacacatac tctcattaac acgggaagaa actatgtttt ccaattctca
       61 ctacatgtac ataatcgttc ttgtactatg gtttagtatt ctcaagtact tacagagaaa
      121 gaatagcata ttacatcctt tattttcact aatcctgaca ccactaacca ttttaatcat
      181 tagacttatg atagatttaa catacaacca ataaat
//
LOCUS       DAZOYL010000855          214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.868, whole genome shotgun sequence.
ACCESSION   DAZOYL010000855 DAZOYL010000000
VERSION     DAZOYL010000855.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.868"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>214)
                     /locus_tag="ACHUOY_004613"
     CDS             complement(<1..>214)
                     /locus_tag="ACHUOY_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HFR0685133.1"
                     /translation="AGESGRQLLPALMQAKEYFPVAFVDDNPRLHKAVIHGVTVYPSD
                     KLSYLVDRYGIKKILLAMPSVSKSQR"
BASE COUNT           63 a           51 c           41 g           59 t
ORIGIN      
        1 tgcctttgtg acttactgac gctcggcatc gccaaaagaa ttttctttat accatagcga
       61 tctacaaggt aactcagttt atccgaggga taaactgtta caccatgaat gacagcctta
      121 tgcaagcgag gattatcatc cacaaatgcc acaggaaaat attcttttgc ctgcattaat
      181 gctggcaata attgtcggcc agattcacca gcgc
//
LOCUS       DAZOYL010000856          213 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.869, whole genome shotgun sequence.
ACCESSION   DAZOYL010000856 DAZOYL010000000
VERSION     DAZOYL010000856.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.869"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
BASE COUNT           69 a           41 c           36 g           67 t
ORIGIN      
        1 taaagttggt agattgtgac tggcttaaaa aatcattaat taataatagg ttatgtttag
       61 agtgttcccc gcgccagcgg ggataaacca ccatataacc cgttatctct ttctcaagtt
      121 tttatattag cagtacttgt aataagcaaa atatccacgt aacacctcat gttcaaaata
      181 gttctccatg ccagagaggt tcacaattat cga
//
LOCUS       DAZOYL010000857          212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.870, whole genome shotgun sequence.
ACCESSION   DAZOYL010000857 DAZOYL010000000
VERSION     DAZOYL010000857.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.870"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..>212
                     /gene="sctE"
                     /locus_tag="ACHUOY_004614"
     CDS             <1..>212
                     /gene="sctE"
                     /locus_tag="ACHUOY_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFR0685134.1"
                     /translation="MVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLE
                     GLGVDSKKAKMIGSILGAIAGALVLV"
BASE COUNT           60 a           50 c           48 g           54 t
ORIGIN      
        1 ttatggttac ggatgctata gtacaagcag cgaccggcaa ttccttcatg gaacaagccc
       61 tgaatccgat catgaaagca gtcattgaac ccttaatcaa actcctttca gatgcattta
      121 caaaaatgct cgaaggcttg ggcgtcgact cgaaaaaagc caaaatgatt ggctctattc
      181 tgggggcaat cgcaggcgct cttgtcctgg tt
//
LOCUS       DAZOYL010000858          211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.denovo.871, whole genome shotgun sequence.
ACCESSION   DAZOYL010000858 DAZOYL010000000
VERSION     DAZOYL010000858.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.denovo.871"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..>211)
                     /locus_tag="ACHUOY_004615"
     CDS             complement(<1..>211)
                     /locus_tag="ACHUOY_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0685135.1"
                     /translation="ITRRVYLSKEKEVSIKVVYFINNVAVHNNTIEIPQTVNGGYDFS
                     HLSLKGIVIKDEDLSNSNFAGCRLQ"
BASE COUNT           63 a           40 c           31 g           77 t
ORIGIN      
        1 ttttgtagtc tgcaacctgc aaaattcgaa ttggataaat cttcatcttt aatcacgata
       61 cctttcaggc taaggtgtga aaaatcgtaa ccaccattta ctgtctgtgg aatttcgata
      121 gtattattat ggacggcgac attatttata aaatatacca ccttaatgga tacttccttt
      181 tctttactaa ggtaaactct tctcgtaatt c
//
LOCUS       DAZOYL010000859         8417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.guided.170, whole genome shotgun sequence.
ACCESSION   DAZOYL010000859 DAZOYL010000000
VERSION     DAZOYL010000859.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.guided.170"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            378..536
                     /locus_tag="ACHUOY_004616"
     CDS             378..536
                     /locus_tag="ACHUOY_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0685136.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="ACHUOY_004617"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="ACHUOY_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR0685137.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1872..2675
                     /locus_tag="ACHUOY_004618"
                     /note="aph(3'')-Ib"
     CDS             1872..2675
                     /locus_tag="ACHUOY_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR0685138.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2675..3511
                     /locus_tag="ACHUOY_004619"
     CDS             2675..3511
                     /locus_tag="ACHUOY_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR0685139.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            3817..4059
                     /locus_tag="ACHUOY_004620"
     CDS             3817..4059
                     /locus_tag="ACHUOY_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFR0685140.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(4091..4741)
                     /gene="tetR(A)"
                     /locus_tag="ACHUOY_004621"
     CDS             complement(4091..4741)
                     /gene="tetR(A)"
                     /locus_tag="ACHUOY_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR0685141.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            4847..6046
                     /gene="tet(A)"
                     /locus_tag="ACHUOY_004622"
     CDS             4847..6046
                     /gene="tet(A)"
                     /locus_tag="ACHUOY_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR0685142.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(6013..6201)
                     /locus_tag="ACHUOY_004623"
                     /pseudo
     CDS             complement(6013..6201)
                     /locus_tag="ACHUOY_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6245..6634)
                     /locus_tag="ACHUOY_004624"
     CDS             complement(6245..6634)
                     /locus_tag="ACHUOY_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685143.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6852..7292
                     /locus_tag="ACHUOY_004625"
     CDS             6852..7292
                     /locus_tag="ACHUOY_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR0685144.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(8037..8186)
                     /locus_tag="ACHUOY_004626"
     CDS             complement(8037..8186)
                     /locus_tag="ACHUOY_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685145.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1852 a         2348 c         2324 g         1893 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     1921 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1981 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     2041 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     2101 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     2161 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     2221 ttgatcaatg tcagtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     2281 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
     2341 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     2401 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     2461 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     2521 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     2581 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     2641 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     2701 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     2761 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     2821 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     2881 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     2941 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     3001 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     3061 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     3121 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     3181 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     3241 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     3301 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     3361 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     3421 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     3481 gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg
     3541 aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc
     3601 ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag
     3661 ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg
     3721 cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt
     3781 ccacgctaga tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc
     3841 gcccgcgagc gccaggcgca cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg
     3901 acgccgcagc aggagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg
     3961 acgctagggc agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg
     4021 aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca
     4081 gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc
     4141 ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg
     4201 cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg
     4261 tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag
     4321 tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc
     4381 agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg
     4441 catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc
     4501 cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg
     4561 ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa
     4621 gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc
     4681 cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca
     4741 tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa
     4801 tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacag
     4861 acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat
     4921 gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta
     4981 tggcattctg ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc
     5041 gctgtcggat cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt
     5101 cgactacgcc atcatggcga cggcgccttt cctttgggtt ctctatatcg ggcggatcgt
     5161 ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg atatcactga
     5221 tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt tcgggatggt
     5281 cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc cgttcttcgc
     5341 cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc cggagtcgca
     5401 caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt cgttccggtg
     5461 ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca tgcaacttgt
     5521 cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc actgggacgc
     5581 gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg cccaggcaat
     5641 gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat
     5701 tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga tggcgttccc
     5761 gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag caatgttgtc
     5821 caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg cgctcaccag
     5881 cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt ctataacaac
     5941 gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct
     6001 gcgtcgcggg ctttggagaa attcttcaaa ttcccgttgc acatagcccg gcaattcctt
     6061 tccctgctct gccataagcg cagcgaatgc cgggtaatac tcgtcaacga tctgatagag
     6121 aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc ggctggccgt
     6181 cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac atacagtcta
     6241 tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct agacattgcc
     6301 agccagtgcc cgtcactccc actcttacat tgttcgcagt atctaggtac atgttattgc
     6361 gtgcaccttc atgatgtata atgttattaa tgacagcaga aagatcgaca gaattaatat
     6421 tcttcgtacc tgggacagtt atcgggtgtc ggccagtgat gagaccaaag gatgcagtat
     6481 aagatgaacc actactatta tcatggccac cattaccacc accgttaaaa ctacctcccg
     6541 aacttccaga gccgctatga ccatcattgt aggggtcatt tggtcccatg tgaccaaaac
     6601 caccgacagt accgctattt aactctgtac tcataaaatt atcctctttg acttttaaaa
     6661 acagcaaaaa ctgtatttat aacaatgtgt ttatatacag tattcgaaat actacgcccg
     6721 tcaaaaccac accgtcaaca ggttttttgt gtatttttca cgcctcacgg gcagcctcag
     6781 accttttgtg ttaaaacagt aatatgttct gctgttacaa tacggtacac acaggagaca
     6841 ctgtcacttt tatggcatgg cacacagttc aagagacact gaaattaaca ggaaagtcgc
     6901 gctcgcaatt atatcgtgac atggctaaag gccttgtcag ctatcgcaca ggaaaaaatg
     6961 atcgccggga gttcgagacc agtgaactga ttcgggccta tggcgagctg cgcacttctg
     7021 agacaccaaa aaggcacaag gagggacact ccaaatctgt cgttagccag gcggaaaatg
     7081 ctcagctaga cgtcattcag cagaaactgg acgctctgca gcagactgtt accctaatgc
     7141 ttgaagacaa gatggcgcgg gaagcagaaa accgcctgcg aaatgaggag agggaacaac
     7201 tacaggccga aattagccgt ctggctgacg cgctggcaca ggaaaaaaag cggggattct
     7261 ggtcgcggct gtttcgctcc ggttcagagt aacagtgcag gtgactctga tttaccataa
     7321 catctgttat cagcaccgcc tgagaggccc tcagggcgtt tctgacggga aaccggtaaa
     7381 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg
     7441 catgagtatg cgcacaagtg catagcgcgg aaattcacac cattcagccg gggtaagtcc
     7501 tgtttccggc gttcttccca tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
     7561 gtgaagcgtt gccggcgcag ccggaaaacc cgctgtcagc acgttcagga agggggtgat
     7621 gtacaaatgg cacagaggat gaaagtgacg tatgagctga aggaatgatg taaaagtgat
     7681 gtataagtga tgtaaaagtg atgtatgaac tgaaggagcg atgtaattct gtagtcagga
     7741 agaataaccc gttcatgcag gcatgaacga tacagtgagg aacgcttttt ttgtcacgat
     7801 ctccccttaa cctgcgtaaa tcacccgcca tggttgatta tttttcaacc tcaaaatggt
     7861 catacaccta acgcagccgc gattttcatc gcaactgcat gaattaaaag aaaaaaaagg
     7921 aatatttcgt tttcacgagt aatacccggg tattaaaaat ccactgtaat tacatgaaaa
     7981 taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg aaaaagctat
     8041 aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt ctcttcagta
     8101 acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc agtcgaatcc
     8161 agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag gttgttagcg
     8221 ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat actggcttac
     8281 tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga aaaaaggcag
     8341 caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg ctcactgact
     8401 cgctacgctc ggtcgtt
//
LOCUS       DAZOYL010000860         3138 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.guided.358, whole genome shotgun sequence.
ACCESSION   DAZOYL010000860 DAZOYL010000000
VERSION     DAZOYL010000860.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.guided.358"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            26..1159
                     /locus_tag="ACHUOY_004627"
     CDS             26..1159
                     /locus_tag="ACHUOY_004627"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0685146.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1248..1781
                     /gene="blc"
                     /locus_tag="ACHUOY_004628"
     CDS             1248..1781
                     /gene="blc"
                     /locus_tag="ACHUOY_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0685147.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1778..2095)
                     /gene="sugE"
                     /locus_tag="ACHUOY_004629"
     CDS             complement(1778..2095)
                     /gene="sugE"
                     /locus_tag="ACHUOY_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0685148.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2270..2416)
                     /gene="ecnB"
                     /locus_tag="ACHUOY_004630"
     CDS             complement(2270..2416)
                     /gene="ecnB"
                     /locus_tag="ACHUOY_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0685149.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2527..2652)
                     /gene="ecnA"
                     /locus_tag="ACHUOY_004631"
     CDS             complement(2527..2652)
                     /gene="ecnA"
                     /locus_tag="ACHUOY_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0685150.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2704..>3138)
                     /gene="efp"
                     /locus_tag="ACHUOY_004632"
     CDS             complement(2704..>3138)
                     /gene="efp"
                     /locus_tag="ACHUOY_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0685151.1"
                     /translation="RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNE
                     TFEQLSADAKAIGDNAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDT
                     AGTGGKPATLSTGAVVKVPLFVQIGEVIKVDTRSGEYVSRVK"
BASE COUNT          817 a          803 c          777 g          741 t
ORIGIN      
        1 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
       61 cgcctcttgc tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      121 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      181 taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
      241 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      301 cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
      361 tactgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg
      421 cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
      481 aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg
      541 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
      601 tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga
      661 aaagaattac gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt
      721 agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag
      781 caatttaacc ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca
      841 atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg
      901 gccggtcaat cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg
      961 cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac
     1021 gggggcgacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     1081 cgtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctgtcagat
     1141 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     1201 tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc
     1261 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct cctacgccgc
     1321 cgcgtggcgt gacagtagtg aataatttcg acgctaaacg ctatctcggt acctggtatg
     1381 agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca
     1441 gtctgcgtga tgacggcggc ctgaatgtca tcaataaagg ctataaccct gacagagaaa
     1501 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaacacgc gctgcgctga
     1561 aagtgtcatt ctttggtcct ttctatggcg gttataacgt cattgcactt gatcgggaat
     1621 accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc
     1681 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     1741 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     1801 caataatccc caatacgatt aacgccagac tcgccaggcg catcgggtta gcggactcac
     1861 cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat
     1921 aagccgtccc taccggtaac gatttcatcg cccaggcaag tagcgccata ctgactatca
     1981 tcgccgtcac agtaataaca cttggcgtca aacgactaaa gccgtgggta tatttcaggc
     2041 caacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     2101 gctccggaga attggggcca tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag
     2161 ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt acctttgtga
     2221 ctatcgcaaa aaaacaaacg gcacgacata actgtcgtac cgttattgct tattgctgcg
     2281 ctttcgttgc tgcaccagaa atcgcgttac cgccatcaga aatgtcttca ccaacgccac
     2341 gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag aacagaaaag atcgctgcaa
     2401 ttgtcttctt caccataacg tcttcctttt aggtaatgtt atttatgttt gcctatagca
     2461 aattaataat agacaacatc gccaaaaatg atggaaatct gatgattttt cggaagagaa
     2521 gaaaaattag ctggcagcgc gggagatgga gttgccgaga tgtttgatgt cttcgccgaa
     2581 accgcgagcg gtgttacagc ccgtgagcag cgtgctggca agcaaaacaa gaacgataag
     2641 gcgtttcatc atctctgacc tgaataagtg atggtgcagc ctgcaggccg caccacaacc
     2701 gcattacttc acgcgagaga cgtattcacc agagcgggta tccactttga tgacttcgcc
     2761 gatttgtacg aacagcggaa ctttaaccac agcgccagta gacagggtag ccggtttgcc
     2821 gccagtacct gcggtatcac ctttcaggcc cggatcggta tcaacgattt ccagttcaac
     2881 gaagttcggc ggagtaacgg agatcggctg accattccac agagttacga tacactctgc
     2941 ctgatccagc agccatttag cgttgtcgcc aattgctttt gcatcagcag acagctgctc
     3001 gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg ttgtacaggt aagtcaggtt
     3061 catatcgaca acatcagcgc cttcagcgga atcagtagat ttgaaggttt tttctacgcg
     3121 agtaccggtc agcagacg
//
LOCUS       DAZOYL010000861         2844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.guided.372, whole genome shotgun sequence.
ACCESSION   DAZOYL010000861 DAZOYL010000000
VERSION     DAZOYL010000861.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.guided.372"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            79..603
                     /gene="sat2"
                     /locus_tag="ACHUOY_004633"
     CDS             79..603
                     /gene="sat2"
                     /locus_tag="ACHUOY_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0685152.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            672..1169
                     /locus_tag="ACHUOY_004634"
     CDS             672..1169
                     /locus_tag="ACHUOY_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685153.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1230..1601
                     /locus_tag="ACHUOY_004635"
     CDS             1230..1601
                     /locus_tag="ACHUOY_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685154.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            2011..2388
                     /locus_tag="ACHUOY_004636"
     CDS             2011..2388
                     /locus_tag="ACHUOY_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0685155.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            2411..2563
                     /locus_tag="ACHUOY_004637"
                     /pseudo
     CDS             2411..2563
                     /locus_tag="ACHUOY_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(2619..>2844)
                     /locus_tag="ACHUOY_004638"
     CDS             complement(2619..>2844)
                     /locus_tag="ACHUOY_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0685156.1"
                     /translation="LSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
BASE COUNT          752 a          709 c          677 g          706 t
ORIGIN      
        1 attcgcaaac ctgtcacgcc ttttgtacca aaagccgcgc caggtttgcg atccgctgtg
       61 ccaggcgtta ggcgtcatat gaagatttcg gtgatccctg agcaggtggc ggaaacattg
      121 gatgctgaga accatttcat tgttcgtgaa gtgttcgatg tgcacctatc cgaccaaggc
      181 tttgaactat ctaccagaag tgtgagcccc taccggaagg attacatctc ggatgatgac
      241 tctgatgaag actctgcttg ctatggcgca ttcatcgacc aagagcttgt cgggaagatt
      301 gaactcaact caacatggaa cgatctagcc tctatcgaac acattgttgt gtcgcacacg
      361 caccgaggca aaggagtcgc gcacagtctc atcgaatttg cgaaaaagtg ggcactaagc
      421 agacagctcc ttggcatacg attagagaca caaacgaaca atgtacctgc ctgcaatttg
      481 tacgcaaaat gtggctttac tctcggcggc attgacctgt tcacgtataa aactagacct
      541 caagtctcga acgaaacagc gatgtactgg tactggttct cgggagcaca ggatgacgcc
      601 taacaattca ttcaagccga caccgcttcg cggcgcggct taattcagga gttagagagc
      661 tggggaagac tatgcgcgat ctgttgaagg tggttctaag cctcgtactt gcgatggcat
      721 cggggcaggc acttgctgac ctgccaattg ttttagtgga tgaagctcgt cttccctatg
      781 actactcccc atccaactac gacatttctc caagcaacta cgacaactcc ataagcaatt
      841 acgacaatag tccatcaaat tacgacaact ctgagagcaa ctacgataat agttcatcca
      901 attacgacaa tagtcgcaac ggaaatcgta ggcttatata tagcgcaaat gggtctcgca
      961 ctttcgccgg ctactacgtc attgccaaca atgggacaac gaacttcttt tccacatctg
     1021 gcaaaaggat gttctacacc ccaaaagggg ggcgcggcgt ctatggcggc aaagatggga
     1081 gcttctgcgg ggcattggtc gtcataaatg gccaattttc gcttgccctg acagataacg
     1141 gcctgaagat catgtatcta agcaactagc ctgctctcta ataaaatgtt aggcctcaac
     1201 atctagtcgc aagctgaggg gaaccactag tgtcatacga acctccaaga gacggttaca
     1261 caaacgggta cattgttgat gtcatgtatg acaatcgccc aagtaagtat ccagctgtgt
     1321 tcagaacgta cgtccgaatt ccagactatg tcgaactaga taacgcggag gagctggccg
     1381 acgcaatcat tcgagaggtt gagttaaagg aggcatggct cacaagggtc ttggggaact
     1441 cagccgatcc tgacgcttct gtgggcaagt gggaccggcg cctagcgcag tgcggtggta
     1501 ttgatgagat gtcaagtggg gtgcaggcaa catacttcag tccaaacggt cagctggtcg
     1561 gcttagtggc atacattgat gccgaatgga tggatgaata gctctgacgt tacgattcag
     1621 gtgagacaac cagatgggca ttgcgtacaa gctcagttgc caccgatgtg gctacgagtc
     1681 tgatcttctt tacctaggcc aaggtatggc gatgcttcct gagcaaatag tcggaacgtg
     1741 cacatgctgc gacaatctaa cgacgatttc tataaatgaa agctacggag tatgctcact
     1801 ttgtggggct aaggatcgtg tgatatttac aagctatcag catgaggcaa ggccaagaaa
     1861 tccctgctat ctagagagaa gtttcaaata tcaatgccca agatgccatg ccttcagtat
     1921 ggagcctccg gatctaccgg agatgctttg ggactaatgc tccgctttgc ctcgggtatc
     1981 cgttcgacct atgtttaaga ggaaacgcac gtgtctgccg ccaaagctct tgcctatgca
     2041 ctttgtgaca agttggaaag caaaaaagtt gatgaagtcg tacgaaatct tgtttctagc
     2101 ggctggaata ttagggaagt ggcctgggaa gcttcgtcct ttgatcatga aatgccctac
     2161 tttgacacgg ggcgggactt cgatattgga tttcctgaca cgctgtcatc caagattgag
     2221 tttttgaccg cgaatggatc aatcgtccaa gcacgtgctc gcctgcactt caagcaggct
     2281 ctaatttttg ccaaggcgtc caaacatgtc gccgcgctca agaactcatt ggattactac
     2341 tatggatcgt ctgtaatccc ccctcaaaac cacggaggct atttgtagaa aattccatta
     2401 atatgaactc aaggtgttcc ctacggacga atcagcgatg aaggtggtgt acctggctat
     2461 ccaggcggcg accaagaaat ggacgatgcc gatccgcaac tggaagccgg ccatgaaccg
     2521 ttttatgatc gaatttggtg accgtttaaa tggccacctt taactgaccg gcaaaagcac
     2581 ttacacaaaa gggtttacag gctctttcag tcattccttt aatgcgtaaa ttgctctcta
     2641 accactctct cagcccaccc ttccatatcc tctctcgata cttgcagctc ccccaacccc
     2701 tttggatgat tcacccctct atgcccgtct tgtccatata attgatcaaa caaactattt
     2761 ggccaattca atgagctctt caccactcca cgccgtatct tttcaaaatc attccgccat
     2821 gtttcgctat caacgccact caac
//
LOCUS       DAZOYL010000862         2769 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.guided.375, whole genome shotgun sequence.
ACCESSION   DAZOYL010000862 DAZOYL010000000
VERSION     DAZOYL010000862.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.guided.375"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            <1..121
                     /gene="prfH"
                     /locus_tag="ACHUOY_004639"
     CDS             <1..121
                     /gene="prfH"
                     /locus_tag="ACHUOY_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0685157.1"
                     /translation="EQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(178..1635)
                     /gene="pepD"
                     /locus_tag="ACHUOY_004640"
     CDS             complement(178..1635)
                     /gene="pepD"
                     /locus_tag="ACHUOY_004640"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0685158.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            1896..2354
                     /gene="gpt"
                     /locus_tag="ACHUOY_004641"
     CDS             1896..2354
                     /gene="gpt"
                     /locus_tag="ACHUOY_004641"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0685159.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2446..>2769
                     /locus_tag="ACHUOY_004642"
     CDS             2446..>2769
                     /locus_tag="ACHUOY_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0685160.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVW"
BASE COUNT          673 a          708 c          700 g          688 t
ORIGIN      
        1 ggagcagcag caacaggaaa atagcgcggt gctgaaatcg cagcggcgaa tgttccatca
       61 ccagattgaa cgtggcaacc cgagacggac atttacaggg atggctttta tcgaaggata
      121 atgaaggatg aaactgccgg aaggcgatta aacgccatcc ggcagcaaat caaataatta
      181 cttcgccgga atttctttca gcagttcagt cagcagtgtc cagtaatgac ctacgctttc
      241 gatgtgaact tgctcatccg gagagtgtgg accggtgata gttggcccga tagaaaccat
      301 gtccatttcc ggatacggtt ttttgaacag accacattcc aggcccgcgt ggataatctg
      361 gatgttcggc gttttgttga acagacgctg ataggtttca cgtaccagat gcatcaccgg
      421 agaattagcg tccggctgcc agccaggata tgcgcctttc gcttcggttt tcgcgccagc
      481 cagtttaccc agcgaatcca gcatgctcac cacgtagtct ttaccgctgt cgatcagaga
      541 acggatcaga cagtgaattt ctacgttatt atcagtcatg gtcaccacac cgacgttcag
      601 ggaggtttcg accacacctt ttgccacgtc tgagttgcgg atcacaccgt tcggggtggc
      661 gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc
      721 tacagagtcc agcaacaagg ccagattttt ctctttctct gccagctcgt ttttcaggat
      781 ctcctgatag gtattcacca gagatttcag gacgtcgact ttatcagctg cgacagcaat
      841 ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg ccgccgttga aatcgataag
      901 gcgcagatcc agttcttctg catgacccgc taggaagcgc accagcagtt tgttggcatt
      961 acccaggcca acgtggattt ccccgccgga gtgaccccct ttcagacctt ttaaggttaa
     1021 cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa
     1081 gtcgataccc cccgcacaac ccatgtagat ttcaccttct tcttcggagt cggtgttaat
     1141 cagaatatca gcctgcaacc agttgctctg taagccgaac gcaccgtcca taccggcttc
     1201 ttcggtcatg gtcagcagca cttccagcgg gccgtgaacc acgttttcgt cagccagaac
     1261 cgccagcgca gacgccatac caatgccgtt atctgcaccc agcgtggtgc cgcgcgcttt
     1321 aacccattcg ccatcaatat aaggctggat aggatctttc gtgaagtcat gcacggtgtc
     1381 gttatttttc tgcggcacca tatcgaggtg ggcctgtaag acgaccgatt tacgattttc
     1441 catacctgcg gtagcaggtt tacgaatcag gatattacct acctgatcgc gttcgacatg
     1501 gaaacctttc tcttttgccc aaccaacaat gtattcagcg agttgctctt catgatagga
     1561 cgggtgagga atagaacaga ttttggcaaa aatatcccac agcggctgtg gagataattg
     1621 agacagttca gacacgttaa gtctccttgt cgatcacccg caaaacagta ttgcaggtca
     1681 cagggttagc agaaaatgtt gtcaacacaa gacaggcttg cgagatatgt ttgagaatac
     1741 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     1801 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     1861 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     1921 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     1981 ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg
     2041 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     2101 gcgagcttaa agtgctaaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     2161 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
     2221 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
     2281 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     2341 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
     2401 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     2461 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     2521 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
     2581 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgtgaa
     2641 atcctcgacg tccaggcgcg tattgtgatg agcgatgccg aacgtaccga cgatgattta
     2701 tacgatacgg tgattggcta ccgtggtggc aactggattt atgagtgggc catccaggcg
     2761 atggtgtgg
//
LOCUS       DAZOYL010000863          998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2202643
            SAMN43277636-rid23489503.guided.551, whole genome shotgun sequence.
ACCESSION   DAZOYL010000863 DAZOYL010000000
VERSION     DAZOYL010000863.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277636
            Sequence Read Archive: SRR30464485
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396836.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:22:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,644
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,255
            CDSs (with protein)               :: 4,255
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 142 of 311
            Pseudo Genes (incomplete)         :: 113 of 311
            Pseudo Genes (internal stop)      :: 98 of 311
            Pseudo Genes (multiple problems)  :: 39 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277636-rid23489503.guided.551"
                     /strain="M2202643"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-03-31"
                     /collected_by="FSS"
     gene            complement(<1..166)
                     /locus_tag="ACHUOY_004643"
     CDS             complement(<1..166)
                     /locus_tag="ACHUOY_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HFR0685161.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFL"
     gene            498..971
                     /gene="dfrA1"
                     /locus_tag="ACHUOY_004644"
     CDS             498..971
                     /gene="dfrA1"
                     /locus_tag="ACHUOY_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0685162.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
BASE COUNT          319 a          180 c          204 g          295 t
ORIGIN      
        1 ataaaaacag cctgacctct tcactgccca tggtctgagg atgacgtttt ttgtgaaaca
       61 gaataaaacg cttaatccag tgcaggtaag ttttttcagt tttcagcgca taaccttttt
      121 gtcgcatatc cgtgcgtata gaatttaaaa atggactgtt agacataaaa cgctccttgt
      181 cttgcaactg tctgcctata cagcctattc tagctgggat ttaaaaaagt gcctgttttt
      241 tacgcctaga gatgcttgtt taccggtaga gttttaattt aatgctaaat aaattaaaat
      301 gttatgagtt ctttgggtga gataatgtgc atcgtgcaag caggatagac ggcatgcacg
      361 atttgtaata acagagtgtc ttgtattttt aaagaaagtc tatttaatac aagtgattat
      421 attaattaac ggtaagcatc agcgggtgac aaaacgagca tgcttactaa taaaatgtta
      481 acctctgagg aagaattgtg aaactatcac taatggtagc tatatcgaag aatggagtta
      541 tcgggaatgg ccctgatatt ccatggagtg ccaaaggtga acagctcctg tttaaagcta
      601 ttacctataa ccaatggctg ttggttggac gcaagacttt tgaatcaatg ggagcattac
      661 ccaaccgaaa gtatgcggtc gtaacacgtt caagttttac atctgacaat gagaacgtat
      721 tgatctttcc atcaattaaa gatgctttaa ccaacctaaa gaaaataacg gatcatgtca
      781 ttgtttcagg tggtggggag atatacaaaa gcctgatcga tcaagtagat acactacata
      841 tatctagaat agacatcgag ccggaaggtg atgtttactt tcctgaaatc cccagcaatt
      901 ttaggccagt ttttacccaa gacttcgcct ctaacataaa ttatagttac caaatctggc
      961 aaaagggtta acaagtggca gcaacggatt cgcaaacc
//
